LOCUS       DBNENW010000001        71125 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNENW010000001 DBNENW010000000
VERSION     DBNENW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 71125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.001"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            99..2171
                     /gene="lip2"
                     /locus_tag="AC1LW8_000001"
     CDS             99..2171
                     /gene="lip2"
                     /locus_tag="AC1LW8_000001"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HIP4771873.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTN
                     AAVQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQLSKYQSTQ
                     NSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAVADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(2413..3240)
                     /locus_tag="AC1LW8_000002"
     CDS             complement(2413..3240)
                     /locus_tag="AC1LW8_000002"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4771874.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(3313..4512)
                     /locus_tag="AC1LW8_000003"
     CDS             complement(3313..4512)
                     /locus_tag="AC1LW8_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HIP4771875.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4608..4700
                     /locus_tag="AC1LW8_000004"
     CDS             4608..4700
                     /locus_tag="AC1LW8_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771876.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            4814..5815
                     /locus_tag="AC1LW8_000005"
     CDS             4814..5815
                     /locus_tag="AC1LW8_000005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIP4771877.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5849..6181
                     /locus_tag="AC1LW8_000006"
     CDS             5849..6181
                     /locus_tag="AC1LW8_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIP4771878.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6182..6982
                     /locus_tag="AC1LW8_000007"
     CDS             6182..6982
                     /locus_tag="AC1LW8_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HIP4771879.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            6972..7916
                     /locus_tag="AC1LW8_000008"
     CDS             6972..7916
                     /locus_tag="AC1LW8_000008"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIP4771880.1"
                     /translation="MRSSKWNAMSLLRDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            7894..8916
                     /locus_tag="AC1LW8_000009"
     CDS             7894..8916
                     /locus_tag="AC1LW8_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIP4771881.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRGITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELVDKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVGSL
                     VEMILS"
     gene            9232..10257
                     /locus_tag="AC1LW8_000010"
     CDS             9232..10257
                     /locus_tag="AC1LW8_000010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4771882.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFVLSLGAKFNHQAKEGKLLLDMNRNVSNERAKILLGWEPIATQKEAILAAVDS
                     MAKYHLI"
     gene            complement(10350..11696)
                     /locus_tag="AC1LW8_000011"
     CDS             complement(10350..11696)
                     /locus_tag="AC1LW8_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIP4771883.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISNGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(11711..11995)
                     /locus_tag="AC1LW8_000012"
     CDS             complement(11711..11995)
                     /locus_tag="AC1LW8_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP4771884.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(11997..12440)
                     /locus_tag="AC1LW8_000013"
     CDS             complement(11997..12440)
                     /locus_tag="AC1LW8_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP4771885.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(12445..14400)
                     /locus_tag="AC1LW8_000014"
     CDS             complement(12445..14400)
                     /locus_tag="AC1LW8_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP4771886.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            14612..15031
                     /gene="mepR"
                     /locus_tag="AC1LW8_000015"
     CDS             14612..15031
                     /gene="mepR"
                     /locus_tag="AC1LW8_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HIP4771887.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15138..16493
                     /gene="mepA"
                     /locus_tag="AC1LW8_000016"
     CDS             15138..16493
                     /gene="mepA"
                     /locus_tag="AC1LW8_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HIP4771888.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQDIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16600..17040
                     /locus_tag="AC1LW8_000017"
     CDS             16600..17040
                     /locus_tag="AC1LW8_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HIP4771889.1"
                     /translation="MYKSKILLKYIFSEEPEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17123..18481)
                     /gene="glpT"
                     /locus_tag="AC1LW8_000018"
     CDS             complement(17123..18481)
                     /gene="glpT"
                     /locus_tag="AC1LW8_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HIP4771890.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18793..19719
                     /locus_tag="AC1LW8_000019"
     CDS             18793..19719
                     /locus_tag="AC1LW8_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4771891.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNVYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19733..20794
                     /locus_tag="AC1LW8_000020"
     CDS             19733..20794
                     /locus_tag="AC1LW8_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIP4771892.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAPTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSIASLFYTAETTQGAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSPDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            20808..21374
                     /locus_tag="AC1LW8_000021"
     CDS             20808..21374
                     /locus_tag="AC1LW8_000021"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIP4771893.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21434..22429)
                     /locus_tag="AC1LW8_000022"
     CDS             complement(21434..22429)
                     /locus_tag="AC1LW8_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIP4771894.1"
                     /translation="MASLKNKHFMIGLTLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSNGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22734..23279
                     /locus_tag="AC1LW8_000023"
     CDS             22734..23279
                     /locus_tag="AC1LW8_000023"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4771895.1"
                     /translation="MFGMKVNEQIILKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFSDGNGFQCGIWYEGTLVGVIGLHEINRMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            23546..24400
                     /gene="efeO"
                     /locus_tag="AC1LW8_000024"
     CDS             23546..24400
                     /gene="efeO"
                     /locus_tag="AC1LW8_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HIP4771896.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANIEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            24397..25626
                     /gene="efeB"
                     /locus_tag="AC1LW8_000025"
     CDS             24397..25626
                     /gene="efeB"
                     /locus_tag="AC1LW8_000025"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HIP4771897.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDKVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            25607..27319
                     /locus_tag="AC1LW8_000026"
     CDS             25607..27319
                     /locus_tag="AC1LW8_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIP4771898.1"
                     /translation="MKHYLIKFVAMLITAAMVCSFGLLKSQAAKQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAVMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLYTAKVSHAWTTFKSNIDHVDK
                     KSDTSAKDQYHVSQLNDELEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPTYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(27411..28112)
                     /gene="tatC"
                     /locus_tag="AC1LW8_000027"
     CDS             complement(27411..28112)
                     /gene="tatC"
                     /locus_tag="AC1LW8_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIP4771899.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCLISPVMFYQLWAFVAPGLHNNERQFIYKYSFFS
                     VLLFIFGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFIGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     TFIVKFTYRGKPPTH"
     gene            complement(28129..28353)
                     /locus_tag="AC1LW8_000028"
     CDS             complement(28129..28353)
                     /locus_tag="AC1LW8_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIP4771900.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(28452..28841)
                     /locus_tag="AC1LW8_000029"
     CDS             complement(28452..28841)
                     /locus_tag="AC1LW8_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HIP4771901.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSTIPVAQKSNQSIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            29081..29284
                     /locus_tag="AC1LW8_000030"
     CDS             29081..29284
                     /locus_tag="AC1LW8_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4771902.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEV"
     gene            29281..29994
                     /locus_tag="AC1LW8_000031"
     CDS             29281..29994
                     /locus_tag="AC1LW8_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIP4771903.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYTNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            30019..30861
                     /locus_tag="AC1LW8_000032"
     CDS             30019..30861
                     /locus_tag="AC1LW8_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4771904.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPRQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEITKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            30861..31490
                     /locus_tag="AC1LW8_000033"
     CDS             30861..31490
                     /locus_tag="AC1LW8_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIP4771905.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(31880..33013)
                     /locus_tag="AC1LW8_000034"
     CDS             complement(31880..33013)
                     /locus_tag="AC1LW8_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIP4771906.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            33552..34733
                     /locus_tag="AC1LW8_000035"
     CDS             33552..34733
                     /locus_tag="AC1LW8_000035"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIP4771907.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(34816..35568)
                     /locus_tag="AC1LW8_000036"
     CDS             complement(34816..35568)
                     /locus_tag="AC1LW8_000036"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIP4771908.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(35611..37839)
                     /gene="metE"
                     /locus_tag="AC1LW8_000037"
     CDS             complement(35611..37839)
                     /gene="metE"
                     /locus_tag="AC1LW8_000037"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIP4771909.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(37836..39677)
                     /locus_tag="AC1LW8_000038"
     CDS             complement(37836..39677)
                     /locus_tag="AC1LW8_000038"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIP4771910.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIHEGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDNAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(39646..40806)
                     /gene="metC"
                     /locus_tag="AC1LW8_000039"
     CDS             complement(39646..40806)
                     /gene="metC"
                     /locus_tag="AC1LW8_000039"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase MetC"
                     /protein_id="HIP4771911.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(40803..41906)
                     /locus_tag="AC1LW8_000040"
     CDS             complement(40803..41906)
                     /locus_tag="AC1LW8_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4771912.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYNIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(41972..42341)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42570..43415
                     /locus_tag="AC1LW8_000041"
     CDS             42570..43415
                     /locus_tag="AC1LW8_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIP4771913.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFLRIIEQLN
                     RRYGK"
     gene            43572..44453
                     /locus_tag="AC1LW8_000042"
     CDS             43572..44453
                     /locus_tag="AC1LW8_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIP4771914.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            44483..44686
                     /locus_tag="AC1LW8_000043"
     CDS             44483..44686
                     /locus_tag="AC1LW8_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIP4771915.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            44698..45795
                     /gene="ychF"
                     /locus_tag="AC1LW8_000044"
     CDS             44698..45795
                     /gene="ychF"
                     /locus_tag="AC1LW8_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIP4771916.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(45881..46072)
                     /locus_tag="AC1LW8_000045"
     CDS             complement(45881..46072)
                     /locus_tag="AC1LW8_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771917.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            46316..46612
                     /gene="rpsF"
                     /locus_tag="AC1LW8_000046"
     CDS             46316..46612
                     /gene="rpsF"
                     /locus_tag="AC1LW8_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIP4771918.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            46633..47136
                     /gene="ssb"
                     /locus_tag="AC1LW8_000047"
     CDS             46633..47136
                     /gene="ssb"
                     /locus_tag="AC1LW8_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP4771919.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            47188..47430
                     /gene="rpsR"
                     /locus_tag="AC1LW8_000048"
     CDS             47188..47430
                     /gene="rpsR"
                     /locus_tag="AC1LW8_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIP4771920.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(47696..48634)
                     /locus_tag="AC1LW8_000049"
     CDS             complement(47696..48634)
                     /locus_tag="AC1LW8_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIP4771921.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNHFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(48685..49387)
                     /locus_tag="AC1LW8_000050"
                     /pseudo
     CDS             complement(48685..49387)
                     /locus_tag="AC1LW8_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466541.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            49593..50204
                     /locus_tag="AC1LW8_000051"
     CDS             49593..50204
                     /locus_tag="AC1LW8_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin beta-grasp domain-containing protein"
                     /protein_id="HIP4771922.1"
                     /translation="MFKKNDSKNSILLKSILSLGIIYGGAFGIYPKADASTQNSPSVQ
                     DKQFQKVEEVPNNSEKALVKKLYDRYSQNTINGKSNKSRNWVYSERPLNENQVRINLE
                     GTYRVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            50573..50945
                     /locus_tag="AC1LW8_000052"
                     /pseudo
     CDS             50573..50945
                     /locus_tag="AC1LW8_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
     gene            51122..51694
                     /locus_tag="AC1LW8_000053"
     CDS             51122..51694
                     /locus_tag="AC1LW8_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIP4771923.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(51832..52095)
                     /locus_tag="AC1LW8_000054"
     CDS             complement(51832..52095)
                     /locus_tag="AC1LW8_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4771924.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            52389..52640
                     /locus_tag="AC1LW8_000055"
     CDS             52389..52640
                     /locus_tag="AC1LW8_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIP4771925.1"
                     /translation="MFGFIGMLIIGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            complement(52679..52888)
                     /locus_tag="AC1LW8_000056"
     CDS             complement(52679..52888)
                     /locus_tag="AC1LW8_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771926.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            53066..53647
                     /locus_tag="AC1LW8_000057"
     CDS             53066..53647
                     /locus_tag="AC1LW8_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP4771927.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(53713..54096)
                     /locus_tag="AC1LW8_000058"
     CDS             complement(53713..54096)
                     /locus_tag="AC1LW8_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771928.1"
                     /translation="MKVRITFIILAILSTIVCLFLTAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFVSAIITLAIGGKSVDKNDFH"
     gene            complement(54351..54977)
                     /locus_tag="AC1LW8_000059"
     CDS             complement(54351..54977)
                     /locus_tag="AC1LW8_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HIP4771929.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILENKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(55050..56660)
                     /locus_tag="AC1LW8_000060"
     CDS             complement(55050..56660)
                     /locus_tag="AC1LW8_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771930.1"
                     /translation="MLDVHFIYPRKLQNACEYFNLPTQKEGFEVTMHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQLNEDEFFVNNFFLRY
                     INSEVLDNAYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNATLFEFDILSSILESDDNYRL
                     NNELTKFEKLQLINFDNFLTIHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVNEQTIENSLAEFKNALELSFMEDEVDLDMLLEDEDFGTTDSLRSDAITQLIS
                     EIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLLQRFYDATFSKEELQYSLDTLA
                     GTELYYVEDDFVFHPVLFDVVESEASGIDISDDGYYVPASVEELIYYDRHHYLKSDQA
                     IKEITSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLIQPIMQLFIDNAILNNVQN
                     DALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            complement(56775..58298)
                     /gene="ahpF"
                     /locus_tag="AC1LW8_000061"
     CDS             complement(56775..58298)
                     /gene="ahpF"
                     /locus_tag="AC1LW8_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HIP4771931.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(58314..58883)
                     /gene="ahpC"
                     /locus_tag="AC1LW8_000062"
     CDS             complement(58314..58883)
                     /gene="ahpC"
                     /locus_tag="AC1LW8_000062"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HIP4771932.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            59375..60130
                     /gene="nfsA"
                     /locus_tag="AC1LW8_000063"
     CDS             59375..60130
                     /gene="nfsA"
                     /locus_tag="AC1LW8_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HIP4771933.1"
                     /translation="MSEYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYNQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(60211..61599)
                     /locus_tag="AC1LW8_000064"
     CDS             complement(60211..61599)
                     /locus_tag="AC1LW8_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HIP4771934.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            61684..61782
                     /locus_tag="AC1LW8_000065"
     CDS             61684..61782
                     /locus_tag="AC1LW8_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771935.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
     gene            complement(62773..63729)
                     /locus_tag="AC1LW8_000066"
     CDS             complement(62773..63729)
                     /locus_tag="AC1LW8_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771936.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKADNAGDGTTT
                     KVDKEDQISATEAIEKASVEQSKNGNAAETSNKEATIDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(63847..64509)
                     /locus_tag="AC1LW8_000067"
     CDS             complement(63847..64509)
                     /locus_tag="AC1LW8_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771937.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            complement(64652..65059)
                     /locus_tag="AC1LW8_000068"
     CDS             complement(64652..65059)
                     /locus_tag="AC1LW8_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HIP4771938.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     regulatory      65239..65341
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            65572..66150
                     /gene="xpt"
                     /locus_tag="AC1LW8_000069"
     CDS             65572..66150
                     /gene="xpt"
                     /locus_tag="AC1LW8_000069"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIP4771939.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     gene            66150..67418
                     /gene="pbuX"
                     /locus_tag="AC1LW8_000070"
     CDS             66150..67418
                     /gene="pbuX"
                     /locus_tag="AC1LW8_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease PbuX"
                     /protein_id="HIP4771940.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            67456..68922
                     /gene="guaB"
                     /locus_tag="AC1LW8_000071"
     CDS             67456..68922
                     /gene="guaB"
                     /locus_tag="AC1LW8_000071"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIP4771941.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            68947..70488
                     /gene="guaA"
                     /locus_tag="AC1LW8_000072"
     CDS             68947..70488
                     /gene="guaA"
                     /locus_tag="AC1LW8_000072"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIP4771942.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCECKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
BASE COUNT        24024 a        10834 c        13440 g        22827 t
ORIGIN      
        1 agtcttaatc aaaaaataat atatgtcatg ttatattgaa ggtgcagttg tttttcattc
       61 tcaagagggg gtcaaaaaaa tacttttgag gtgattatat gttaagagga caagaagaaa
      121 gaaagtatag tattagaaag tattcaatag gcgtggtgtc agtgttagcg gctacaatgt
      181 ttgttgtgtc atcacatgaa gcacaagcct cggaaaaaac accaactaat gcagcggtac
      241 aaaaagaaac actaaatcaa ccgggagaac aagggaatgc gataacgtca catcaaatgc
      301 agtcaggaaa gcaattagac gatatgcata aagagaatgg taaaagtgga acagtgacag
      361 aaggtaaaga tacgcttcaa ttatcgaagt atcaatcaac acaaaatagt aaaacaatca
      421 gaacgcaaaa tgataatcaa gtaaagcaag attctgaacg acaaggttct aaacagtcac
      481 accaaaataa tgcgactaat aatactgaac gtcaaaatga tcaggttcaa aatacccatc
      541 atgctgaacg taatggatca caatcgacaa cgtcacaatc gaatgatgtt gataaatcac
      601 aaccatccat tccggcacaa aaggtattac ccaatcatga taaagcagca ccaacttcaa
      661 ctacaccccc gtctaatgat aaaactgcac ctaaatcaac aaaagcacaa gatgcaacca
      721 cggacaaaca tccaaatcaa caagatacac atcaacccgc gcatcaaatc atagatgcaa
      781 agcaagatga tactgttcgc caaagtgaac agaaaccaca agttggcgat ttaagtaaac
      841 atatcgatgg tcaaaattcc ccagagaaac cgacagataa aaatactgat aataaacaac
      901 taatcaaaga tgcgcttcaa gcgcctaaaa cacgttcgac tacaaatgca gtagcagatg
      961 ctaaaaaggt tcgaccactt aaagcgaatc aagtacaacc acttaacaaa tatccagttg
     1021 tttttgtaca tggattttta ggattagtag gcgataatgc acctgcttta tatccaaatt
     1081 attggggtgg aaataaattt aaagttatcg aagaattgag aaagcaaggc tataatgtac
     1141 atcaagcaag tgtaagtgca tttggtagta actatgatcg cgctgtagaa ctttattatt
     1201 acattaaagg tggtcgcgta gattatggcg cagcacatgc agctaaatac ggacatgagc
     1261 gctatggtaa gacttataaa ggaatcatgc ctaattggga acctggtaaa aaggtacatc
     1321 ttgtagggca tagtatgggt ggtcaaacaa ttcgtttaat ggaagagttt ttaagaaatg
     1381 gtaacaaaga agaaattgcc tatcataaag cgcatggtgg agaaatatca ccattattca
     1441 ctggtggtca taacaatatg gttgcatcaa tcacaacatt agcaacacca cataatggtt
     1501 cacaagcagc tgataagttt ggaaatacag aagctgttag aaaaatcatg ttcgctttaa
     1561 atcgatttat gggtaacaag tattcgaata tcgatttagg attaacgcaa tggggcttta
     1621 aacaattacc aaatgagagt tacattgact atataaaacg cgttagtaaa agcaaaattt
     1681 ggacatcaga cgacaatgct gcctatgatt taacgttaga tggctctgca aaattgaaca
     1741 acatgacaag tatgaatcct aatattacgt atacgactta tacaggtgta tcatctcata
     1801 ctggtccatt aggttatgaa aatcctgatt taggtacatt tttcttaatg gatacaacga
     1861 gtagaattat tggtcatgat gcaagagaag aatggcgtaa aaatgatggt gtcgtaccag
     1921 tgatttcgtc attacatccg tccaatcaac catttgttaa tgttacgaat gatgaacctg
     1981 ccacacgcag aggtatctgg caagttaaac caatcataca aggatgggat catgtcgatt
     2041 ttatcggtgt ggacttcctg gatttcaaac gtaaaggtgc agaacttgcc aacttctata
     2101 caggtattat aaatgacttg ttgcgtgttg aagcgactga aagtaaagga acacaattga
     2161 aagcaagtta aattcatctt ctgaatttaa tatgctatgt aaatcgtgct gttatcatgg
     2221 cacatcagat ataagtagca tcacagtgtt gaatttaaaa atagtaaagt gaaataaagc
     2281 gcctgtctca ttagcgaaaa ctaaagggac aggcgtatct gtttatgagc ttaataaatt
     2341 gtatgaataa tatggttgat cgaataactg tttatcatga tgataaattg agttttttaa
     2401 aataatgata tattacatca ttgttatagc gtttaagaaa tcaacaactt tacgataaat
     2461 agtgattgct tcgtcattag gtctacgatc aaaatcatgc tcgtttttat tcacgcgttc
     2521 aaatgttgaa tgtggaacat gattcatgat atgttcgctt tcctcaacgg gaacatcata
     2581 atcgccatta caatgcgcaa tgaaaacagg tggaagtgtt ttaagttcat ctggtgcaat
     2641 attatatttt gaatcagtat aatcagcaat gttaatcata tttatccatt tacctgtgcc
     2701 acgtgcataa acgtagatta aaaaacgttg tgcgatttga tcttgaacaa ccggtgttgg
     2761 tgaagtgagt tgtgcaatca ttgtttcgtt tacgctttga gctatttttg cgtaataact
     2821 attagttgtt ttaaaaggtt cagtgttgat gcgactataa ccataaaaat caataacacc
     2881 atcaatatct ctgtctcgtg caattaatag acttaaatat gcacctgatg atctgccaaa
     2941 ggtaaaaata gggcaattag aatattgtga ttgaatcgca tcgaatgatg cgtagacatc
     3001 ctcaataatg caatcgagac ttacttctgg taataaacga taacttagtt gaattaaatc
     3061 gtaatgttcc gtaaggatat cgatatactg tggggataaa tcgttagctt taccgaacat
     3121 taatccacca ccgtggatgt agacaatagc gccttttgtt ggttgatttt ttgctttaat
     3181 aattgtgtaa ggtaatgcaa atgcatcttt agtaattact ttatctttaa tttcagtcac
     3241 gatttaatag gctccttatt tttgatattg atgtcattat aacactgtct taaatttcca
     3301 tgaaaaatag tcttaagacg atgagtcatg ataattctgt tccaattgac gtaaagcgtc
     3361 acgggtatgc ttctttagac cttccccata atccatcatt ttaacaatat ctttaaaagc
     3421 agcatgtgga atggctaaat cttctaaatc tgccatagaa aattcaagat tgatatcatg
     3481 tggtcgctgt tcagcaagtt tatgcacaaa gtcaggttct gtgacaaaag gcgaagacat
     3541 gccgaccata tctgcatgtt gtaaagcatc taaagcagac tctggagaat taatcccgcc
     3601 acttgcaatt aaagggatac gacctgctaa atgttcatag acaatttggt taactggtcg
     3661 accgaaatga tcacctggtg tacgagacgt attttgataa atatgtcgac cccagctagc
     3721 gattgctaag tattggatgt ttgaaacgtc catgacccaa tcgattaatt ggttgaactc
     3781 gtcaatggta tatcctaaat cactgcctct ggtttcttct ggcgttgctc gaaatcctaa
     3841 aataaaattg tcaggtgctt ctttatcaat cacttcttgt accgcacgca taacttctaa
     3901 acataatctt gcacgatttt ttaatgagtc ggcaccgtaa tggtctgtac gtttattcga
     3961 aaaagttgag aaaaatgttt gaatcagcaa acgttgtgca atcgaaattt ccacaccatc
     4021 aaaacctgct ttaatcgcgc gtaatgtagc atcgcgatac tgctgaatga tgctattgat
     4081 tttctcatga gacatggcga taacatcgtg ttcaatcggt gaatgcaatg tcatagggct
     4141 tggtccatac acctttccaa aatttaaaat ggcttgattt gaaaaacgac cagcatgcgc
     4201 tagctggata atagcgaggc taccatgttg tttcatcgta gatgccatgt tagttaatcc
     4261 agggatacaa gcatcatgat caatattaaa gccatattca aacaattgac cataaggttc
     4321 aatgtaagca gcgccggtga cttgcattcc agctgaatta gagcgacgtg cagcataagc
     4381 caagtcttct tttgtaatat agccttcttt tgttgatgtg tttacggtca ttggtgataa
     4441 tacaaagcga ttcgaaattt tgatgccatt aggtaagtgg attgattgta aaagtggttt
     4501 gtatcggtac atactatgat tccttttcta ttcaatattg ttttcaaagt accatggaaa
     4561 gaatgaataa tcaatgatga acagtcttga tagaatagaa ttggtacatg gaaagtattt
     4621 ttaaaattaa actaatgaat ggcatttgta ggtctgaaaa tatgaatatg aaaaagaaaa
     4681 ataaaggcga aaagatataa aagttaattg aaaaacgtta tcatatacgt gggtatatga
     4741 agagggaatg gtattaagaa cgctaaaatg ttatgtcggt ttgacatgac aggataagtt
     4801 tggagatgac ggattggtta aattaagcgt attagactat gccttaatag atgaaggtaa
     4861 ggatgcacaa aaggcattgc aagattcagt gacacttgca aaattagcag atcgacttgg
     4921 ctttaagcga atttggttta cggaacatca taatgtacca gcgtttgcgt gtagtagtcc
     4981 agaacttttg atgatgcata cattggcgca gacaaatcac atacgagttg gctctggtgg
     5041 tgtgatgctg ccgcactatc gaccttataa aattgctgag cattttagaa tgatggcagc
     5101 gttatatcca aatcgtattg atttaggtat tggtaataat ccaggtacta ctatggtaaa
     5161 gcaagcttta gatggaataa atcctacata tgatagttac gatgaatcga tttcgttatt
     5221 acgtgattat cttacaataa aggataaacc aagtgcgcat acgttaggtg tccaaccaca
     5281 cattgatcat tttccagaaa tgtggttatt aagtagtagc gcaacatctg ccaaaatagc
     5341 tgccgaacta ggtatagggc tttctgttgg aacatttttg ctaccagata taaatgcgat
     5401 acatgcagcg aaggataaca ttgatattta caaaaaacat ttccaagcat caacgattaa
     5461 aatggacgca aaggtgatgg catctgtatt tgtcattgta gctgataacg aagcggaagt
     5521 agcagcatta caacatgcct tagatgtttg gttattaggt aaattacaat ttgcagaatt
     5581 tgaagatttt ccttcagtag acacagcaca aaagtataag cttaatgatc gagacaaaga
     5641 gatgattcaa gcacatcaag cacgcatcat tgcaggtaca caagaaaagg ttaaagcaca
     5701 attagatgat ttcattgcta cgtttgaagt tgatgaggtg ttagtagcac cgcttattcc
     5761 aggtattgaa cagcgttgta aaacattaaa attactcgcg gaaatttatt tgtagcattt
     5821 taaatagaag agaaaggatg aagataagat gaaaaagtta gccaattatt tatgggtaga
     5881 aaaagtagga gatttgtatg tgtttagtat gacacctgaa ttgcaagatg atattgggac
     5941 agtaggttat gttgaattcg taagtccaga tgaagttaaa gtggatgatg aaattgtgag
     6001 tatcgaagca tcgaaaacgg tcattgatgt gcaaacgcca ttgtcaggaa cgattattga
     6061 gcgaaataca aaagcggaag aagaaccgac aattttaaac tctgaaaaac cagaagaaaa
     6121 ttggttgttc aaattggatg atgtcgataa agaagcattc ctagcattac cggaggctta
     6181 aatggaaacg ttaaaatcaa ataaagcgag acttgaatat ttaatcaatg atatgcatcg
     6241 agagagaaat gacaatgacg tattggtaat gccatcttca tttgaagatt tgtgggaatt
     6301 atatcgaggc ttagcaaatg tcagaccggc attacctgta agtgatgaat atttagctgt
     6361 acaagatgct atgttaagtg atttgaatcg tcaacatgtt acggatttga aggatttgaa
     6421 gccgataaaa ggtgacaata tctttgtttg gcaaggtgat atcacgacgt taaaaatcga
     6481 tgctattgtt aatgctgcaa atagtcgttt tctaggatgt atgcaagcta atcatgactg
     6541 cattgataat attattcata caaaagcggg tgttcaagtt cgacttgatt gtgcagagat
     6601 cattcgacaa caagggcgca atgaaggtgt aggtaaagcc aaaataacac gtggatataa
     6661 tttgccagca aagtatataa ttcatacggt tggtccgcaa atacgtcgat tgcctgtttc
     6721 aaagatgaat caggacttgt tagctaaatg ttatcttagc tgtcttaaat tggctgatca
     6781 acatagttta aatcatgtcg ctttttgctg tatatctaca ggtgtgtttg cttttcctca
     6841 agatgaagca gcagaaattg ctgttcgaac agtagaaagc tatctcaaag aaacaaattc
     6901 aacattgaaa gtcgtgttca atgtatttac agataaggat ttacaactgt ataaggaggc
     6961 atttaaccgt gatgcggagt agtaagtgga atgcaatgtc tctgttaagg gatgacaaga
     7021 caaagcaggc tgaagtattg cgtactgcga ttgatgaagc agatgcgata gtgattggaa
     7081 ttggtgcagg catgtctgca tctgacggat ttacatatgt aggagagcgt tttacggaaa
     7141 atttcccaga ttttattgaa aaatatcgct tctttgatat gttgcaagcg agtttacatc
     7201 cttatggcag ttggcaagag tattgggcat ttgagagtcg ttttattaca ttaaactatt
     7261 tagatcaacc tgtaggtcag tcttacctcg ctttaaaatc cttggtggaa ggtaaacagt
     7321 accacattat aactacgaat gcagataatg ctttcgatgt agctgattat gatatgactc
     7381 atgtatttca tatacaaggg gagtatatac tgcaacagtg tagtcagcat tgtcatgctc
     7441 aaacgtatcg caatgatgat ttaattcgta aaatggttgt tgcgcaacaa gatatgctta
     7501 taccttggga gatgattcca agatgtccaa aatgtgatgc cccaatggaa gtgaataaac
     7561 gtaaagcgga agttgggatg gttgaagatg ctgaatttca tgcgcaacta catcgttata
     7621 atgcttttct agagcaacat caagatgata aagtgttgta tttggaaatt ggaattggtt
     7681 atactacacc acaatttgtg aagcatcctt ttcagcgtat gacacgtaaa aatgaaaatg
     7741 cactttatat gacgatgaat aaaaaggcat atcgcattcc gaattcaatt caagaacgta
     7801 ccatacattt aactgaggat atctcaacat tgattacaac agcactccgg aacgatagca
     7861 caacgcaaaa taacaacatt ggagagacaa aagatgtact taatagaacc gattaggaat
     7921 ggagaatata ttactgatgg tgcgattgca ctcgctatgc aagtttatgt taatcagcat
     7981 atctttttag atgaagatat tttattccct tattattgtg atccaaaagt ggaaattgga
     8041 cgttttcaaa atactgctat agaagtgaat caagattata tagataaaca cagtattcaa
     8101 gtagttcgcc gagatactgg tggtggcgct gtgtatgttg ataaaggtgc cgttaatatg
     8161 tgttgtattt tagaacaaga cacttcaatt tatggtgatt ttcaacgatt ttatcagcca
     8221 gctataaagg cgttgcatac attaggtgca acagatgtga tacaaagcgg tagaaatgat
     8281 ttaacattga atggtaaaaa agtgtcaggc gccgcaatga cattaatgaa caatcgtatt
     8341 tatggcggtt attcgctatt acttgatgtt aattatgaag caatggataa agtgttaaag
     8401 cctaatcgca aaaagattgc atcgaaaggg attaaatctg tgcgcgcacg tgttggtcat
     8461 cttagagaag cactggatga aaagtatcgt ggtataacca ttgaagaatt taaaaattta
     8521 atggtgacac agattttggg aatcgatgac attaaagagg caaaacgata cgaattaacg
     8581 gatgcagatt gggaagcgat tgatgaatta gttgataaaa agtataaaaa ttgggattgg
     8641 aattatggca agtcacccaa atatgaatat aatcgaagtg aaagattatc atcaggtact
     8701 gtagatatta ccatttctgt tgaacaaaat cgtattgcag attgtagaat ttttggtgat
     8761 ttctttggac aaggtgatat aaaagatgtg gaagaagcat tacaaggaac aaaaatgaca
     8821 agagaagatt taacgcatca gttaaagcaa ttagacatcg tttattattt tggtaatgtt
     8881 acggttggat cattggttga gatgatttta agttaatatt gtttaggcgt taatgtcgca
     8941 ccctcaatga atatgtgtat ttgcttttaa aatggtgact atgtagttat gtaatttgaa
     9001 catacgattg aaaatgaatt tagttaatta gcaaagacat catatttata gcgtttgtgt
     9061 ttctaaatga ttgggtattt gaaaatggtt aatgatagtt tttaaaggaa taaattcatt
     9121 ttcatttata taagtaagat taaatactag ctactaaagt ctatttatat acgtcaaaat
     9181 ataatgactg ctataatgag taatcaatag acacaaagag gagattatgt gatgaataat
     9241 aaagtattag taaccggtgg tacagggttt gttggcatgc gaattatttc acgattatta
     9301 gaacaaggtt atgaggtaca aacaacgata cgtgatttaa gtaaagctga taaagtaatt
     9361 aaaacaatgc aagacaatgg catttccaca gagcgattaa tgtttgtcga agcggattta
     9421 tcacaagatg aacattggga tgaagcaatg aaagattgta aatatgtctt gagtgtagca
     9481 tctccggtgt ttttcggtaa aacagacgat gcagaagtga tggcgaagcc tgccattgaa
     9541 ggcatacaac gtattttaag agctgcagaa catgctggcg tgaagcgtgt ggtgatgact
     9601 gcaaactttg gtgcagttgg ttttagcaat aaagataaaa attcaatcac aaatgaaagt
     9661 cattggacaa atgaagatga accaggctta tcagtatatg aaaaatcaaa attgttagct
     9721 gaaaaggcag cgtgggattt tgttgagaat gaaaatacaa cagtagaatt tgccacaatc
     9781 aatccagttg caatttttgg gccatcatta gatgcacacg tttcaggaag ctttcattta
     9841 ttagaaaatt tattgaatgg ttcaatgaaa cgtgtaccgc aaattccatt gaatgttgtt
     9901 gatgtgagag acgtagctga actacacatt ttggcaatga caaatgaaca ggctaatggc
     9961 aagcgattta ttgctacagc tgatggacaa attaatttgt tggaaattgc aaaattaatt
    10021 aaagaaaaga gacctgaaat agctcaaaaa gtttctacta aaaaattacc agactttgtt
    10081 ttgagtctag gtgctaaatt taatcatcaa gctaaagaag gtaaactttt attagatatg
    10141 aatcgaaatg taagtaacga acgtgcaaaa atacttcttg gttgggaacc gattgcgaca
    10201 caaaaagaag caattttagc agctgtcgat agtatggcta agtatcattt aatataatac
    10261 aaaacaccgt ccataagtaa gtggctttcc tcttgataaa agaggcattc actcaatatg
    10321 tgacggtgtt ttatatattt acgttaacac tattgctgtg gcttttgacg gccttttaat
    10381 aaaattgctg tcgcacctac gataataata aatagaatcg caccaaataa tcccatatat
    10441 tttactgcgt taccgaacac gataccgaca gttaaaaagt ctgtatctga gaatgttgtt
    10501 gcagcaccac ctaattcgcc taaaaatggc aagaataata atggtaaaaa cgttattagg
    10561 ataccattta gagcggcgcc agcaacagca cctttaatac cgcctcttgc attaccgaat
    10621 acagcagccg ttgcacctaa gaagaagtgt gcaactacgc caggtaaaat gacgacgcca
    10681 ccaaataaga ataagataaa cataccgatg acacctgtaa taaagctgac aaagaatcca
    10741 attaatactg cattttgtgc ataagggaac acaatagggc agtctaatgc aggtttagaa
    10801 tttggtacaa gcttttcaga aattccttta aatgctggga cgatttcagc taagattaag
    10861 cgaacgcccg ttaaaataat aaatacacca gcagcaaatg tcacaccttg aattaatgaa
    10921 aagacaataa agttttgacc attactaata gactcgtgta cataactaac gcccgcaaat
    10981 aagcatgcga tgaagtaaag taatgccatc gtaatcgaga tactaattgt actttctcgt
    11041 aagaaactta agccttttgg aaatttaatc tcttccgttg atttagactt acctttgaat
    11101 aattgaccta cagcacctgc ggcaaagtaa ctgattgagc caaaatgacc taaagctact
    11161 tggtcattcc ctgtaatttt tcgcatcgta ggttggagta atgcaggtaa tactgccatg
    11221 attaatccta atacgagtgc gccgataaca atcgttagcc agcctttaat atgactgact
    11281 gttaaaatga ttgctaaaaa cgcagccatg taaaatgtat gatgacctgt taaaaagata
    11341 tattttaaat tagtgaagcg ggcaattaaa atattaacaa tcatgccaca gaccatgatg
    11401 agtgcagctg ttgttccaaa atcttttaag gctagtgaga cgatagcttc gttgttaggt
    11461 acgatacctt gcacaccaaa tgcgtgttgg aatattttgc cgaatggttc aagagatcga
    11521 acgacgacat cagcacctgc acttaaaatt aagaagccta atatcgtttt aatggttcct
    11581 gaagtgatcg ttgcggcagg ttttttctga acgattaaac ctataaaggc aatcagtgca
    11641 acaagaatgg ctggttgact taaaatatcg actataaaat taaggattgc ttgcataggt
    11701 cgtacctcct ttaaatcatg ttaagttgtt gtaatttttc tgagagcttt tgttgtaatt
    11761 cagctttatc taaaatatta tcaagaacta agacatcccc tagacgttcg gcattttcag
    11821 ctaaatctct accacaaata aacaagtcag ccatctctgg acttgctgtc ataatgtcac
    11881 tatgttcaac ttcgatatca gatggtgcat taagttgcct aagtgcttct tgtgcgttca
    11941 tttctaccat aaaactactt cctaaaccgt ggccacatac tactaaaatt ttcatattaa
    12001 tcatgctcct ttaaaatgtt tttaatgtct tgtgcatttg ttgcagttaa tagttgctgg
    12061 actgtttggt tatcgcccag tacggttgct aaattttgta atacagataa gtgtgaatga
    12121 ttgtcgatgg cactcaatac aaaaatgaga gatgcgtagt gatcttcatc acaaaatgcc
    12181 acatgttgat tcaactttaa tagacttaaa ccaacttgat gtacgtcatt gttcggtctt
    12241 gcatgtgcaa ttgcaatttc aggtgcgata acgatataag gtccaagttc attaacgcta
    12301 tcaatcattg cttgaacata gccttgttca ataatttgtt cttgtagtaa tggctgagaa
    12361 gctatagtta tagcttcagt ccaatcattt acttgttctt ttacaatgat gcgtgttgtt
    12421 gacaaaatgt ctaatgacac gttattaagc ctcctttgtc atagttaaag caatgtgttg
    12481 tttaatttta aaaatattcc catctaagaa atcttgtcga tataagtcgt tgcttaagca
    12541 ttcgcttaac tgtcccaatg cctttaaatg tgcattgggg tggtccgttg ctaatgtaat
    12601 tacaaggtga acgggatcgt tagctttact accaaagata atcccttcag tgaaatatgt
    12661 tagtgcgaaa cctacaccat tctgtacata atcagtacca gcgtgaataa gtgcaatatg
    12721 tggactaatg accatatatg acccgaattg ttcaaattgt tttaaaattg cagctgtata
    12781 atttgaatag acaatgccat cattgattaa aggttgcaca gccactgcaa ttgcggattc
    12841 aattgataat ggttgtttat ttataatgat gcgatgttca ggcaataaat ctgcgagtga
    12901 cttgccatca gttgccattt tcatgactcg ttgttctctt gagtcattga taatttgatt
    12961 caatttttga cgagattgtt gattgataaa tggatcgaca tgaataactg gtacagctga
    13021 tatttcacaa ggtactgttg aaatgacata atcaatgtta tcttgcaata atcgactttc
    13081 ttccaattga taaatggaat aggcatccca aatgtgaaac tcaggataca ggtgatttag
    13141 ttttgatttt aaaagttgtg acgtgcctat accagaacca catagtaaga caaccttaat
    13201 cattgattgt ttatgtgttg caacacgctc tatacttgat gcgaagtgaa ttgtaatgta
    13261 tgttaattca tcttcgttga agcgaatagc agcatcttgt tcaattggac taatatgctt
    13321 gctaacggct tcaatgattt gaggatagcg acgcataact tcttgcctca aaggattagg
    13381 ttgtagcata tcgtatttaa tacgatgtat agctggtttg atatgtgtga tcagactggt
    13441 atgtaacttg ttgtcttttg acatatcaat gcctaattct tggctaacac aagtgatcaa
    13501 ttcatgtata ttttgcgata aatcatggta ttcaaaggta attgaagatg ctgtatgttc
    13561 agtcatttta gagcctagta aatgtaacgt gataaagata atttcagact ctggaaatgt
    13621 gacattacaa ctgcgttcta agttttctat catttttgaa gcaatagcat actgattagt
    13681 atgtcgccat ttatcaattt cattgatagg tatatcgaac gaaaaatttt catttaaacg
    13741 ctgaatggca atgagtatat gatagattaa gccatcgata gccgactgaa ctaaatgata
    13801 attttcacta tttaatgtct taataatggc acggcgaacc aatgcgattg attctgaatt
    13861 aaagatatcc gcctctataa aaggtgcagc ttgtttcata tattgatgta taaagtgtgc
    13921 atacgcttta cgataatgat cttcctcacc aataatattg aatcctttat tgtggacata
    13981 atttaacttt aaatggtatt gatctagttg ggcttgaatc attttaatat catctgcaat
    14041 tgtccgacgc gaaacattaa catcttgcgc aagttgcttt gttgaaacag gatcggttgt
    14101 ttcgaataac tttaaagcga tatgtgtgag tcgttcatct tttgaaaaat gaatttgatt
    14161 tgttagattg tgctctaatt catttaatag tgtcgcgtga gctgttgtta ctttgatgcc
    14221 tgcagcttta ttacggctga cttggtaatg ataagtttca gcatattgct caatatatgc
    14281 tatatcatat tgaatggtac gaggtgatac accaagttga ttagcaatgg tattgattgg
    14341 aataaacgtt tgctcatgaa ttaaaagata caaaatttcg atttgtctat aacttaacaa
    14401 cgtaatatcc tcctatttgt aattgtaagc gatttcttaa aaacgtagat atgcaatctc
    14461 tttcatattt taatccgaaa aattgcatat caaaatgttt atggcgcaag attttatagg
    14521 aacttttaaa ataaattaga tattcatgtt gacaatttaa aaatgtcgca gtatatttag
    14581 ttagacatct aacgaaatgg tggtgcaata aatggaattc acttattcgt atttatttag
    14641 aatgattagt catgagatga aacaaaaggc tgatcaaaag ttagagcaat ttgatattac
    14701 aaatgagcaa ggtcatacgt taggttatct ttatgcacat caacaagatg gactgacaca
    14761 aaatgatatt gctaaagcat tacaacgaac aggtccaact gtcagtaatt tattaaggaa
    14821 ccttgaacgt aaaaagctga tctatcgcta tgtcgatgca caagatacga gaagaaagaa
    14881 tatagggctg actacctctg ggattaaact cgtagaagca ttcacttcga tatttgatga
    14941 aatggaacaa acactcgtat cgcagttatc tgaagaagaa aatgaacaaa tgaaagcaaa
    15001 cttaactaaa atgttatcta gtttacaata aatgataagt gtgactggta gaaatcagtc
    15061 actttgtctt taatattata gttagatatc taattgttag taagctaatt attggaaaag
    15121 acaaggagta ttgaacaatg aaagacgaac aattatatta ttttgagaaa tcgccagtat
    15181 ttaaagcgat gatgcatttc tcattgccaa tgatgatagg gactttatta agcgttattt
    15241 atggcatatt aaatatttac tttataggat ttttagaaga tagccacatg atttctgcta
    15301 tctctctaac actgccagta tttgctatct taatggggtt aggtaattta tttggcgttg
    15361 gtgcaggaac ttatatttca cgtttattag gtgcgaaaga ctatagtaag agtaaatttg
    15421 taagtagttt ctctatttat ggtggtattg cactaggact tatcgtgatt ttagttactt
    15481 taccattcag tgatcaaatc gcagcaattt taggggcgag aggtgaaacg ttagctttaa
    15541 caagtaatta tttgaaagta atgtttttaa gtgcaccttt tgtaattttg ttcttcatat
    15601 tagaacaatt tgcacgtgca attggagcac caatgatttc tatgattggt atgttagcta
    15661 gtgtaggctt aaatattatt ttagatccaa ttttaatttt tggttttgat ttaaacgttg
    15721 ttggtgcagc tttgggtact gcaatcagta atgttgctgc tgctctgttc tttatcgttt
    15781 attttatgaa aaatagtgac gttgtgtcag ttaatattaa acttgcgaaa cctaataaag
    15841 aaatgctttc tgaaatcttt aaaatcggta ttcctgcatt tttaatgagt atcttaatgg
    15901 gattcacagg attagtttta aatttatttt tagcacatta tggaaacttc gcgattgcaa
    15961 gttatggtat ctcatttaga cttgtgcaat ttccagaact tattatcatg ggattatgtg
    16021 aaggtgttgt accactaatt gcatataact ttatggcaaa taaaggtcgt atgaaagacg
    16081 ttatcaaagc agttatcatg tctatcggtg ttatctttgt tgtatgtatg attgctgtat
    16141 ttacaattgg acatcatatg gtcggactat ttactactga tcaagacatc gttgagatgg
    16201 cgacatttat tttgaaagta acaatgacat cattgttatt aaatggtata ggtttcttgt
    16261 ttactggtat gcttcaagcg actgggcaag gtcgtggtgc tacaattatg gccattttac
    16321 aaggtgcgat tatcattcct gtattattta ttatgaatgc tttgttcggc ctcacaggtg
    16381 tcatatggtc attattaatt gctgagtcac tttgtgcttt ggcagcaatg ttaatcgtct
    16441 atttattacg tgatcgtttg acagttgata catctgaatt aatagaaggt taaatatttc
    16501 gtacacttct ggctgagtat atttcggtcg gaagtgtatt tttcgaataa aaaataaata
    16561 tatgatacga ttatgaaaaa aataaagtga gaatgacata tgtataaatc taaaatactg
    16621 ttgaaatata tttttagtga agaaccagaa gttaaagatt taactgaaga aaaatataat
    16681 caagattacg aagcattaac atttagcttt aaagaggaaa catatcaaag taggttagct
    16741 aagaaaacac cgactaaagc agggtatttt gttacatgtt ggacaaaaga cgagaacaat
    16801 tgtaatcaac catactcaaa agaggcattt gcagattact taatgattat agttattgat
    16861 gaagaattaa gcggttattt tttatttcct agggaattat tggtagaaaa aggtatctta
    16921 actacatttg aacataaagg taagatggct tttagagttt atcctaagtg gtgtaatcaa
    16981 ttgaataaaa cagcagggca aacacaaaag tggcaatgta aatatttttt tgaatactaa
    17041 taaaaagtca tatggttgaa cttataagag atgaatatat catctttcga taagataacc
    17101 atatgacttt attttttatc atttaatgat gaacggtttc ttgtcctact ttattccaag
    17161 tgaggataaa gctcaacatt gcaaacacac tgattgctgt taataaaata aaaccgacat
    17221 cccatccgaa tttatcaact acagcaccta agacgatgtt ggccattaca gcaccaaaca
    17281 gataaccaaa taatcctgtt aatccagctg ctgtgccagc tgctttttta ggtacataat
    17341 ctaatgcttg taaaccaatt aacataactg gtccatatat taagaaacca atggcaatta
    17401 atgagacatt gtctaaccaa gcattgcctg gaggatttaa ccaataaatt aatacaaata
    17461 ctgtgacacc taacataaag aagaaacctg caggtcctcg acgacctttg aataatttat
    17521 cagaaatgta accacataat aatgtaccag gaattccagc ccattcgtat aagaagtatg
    17581 cccaacctga tgcttttaag tcgaaatgct tttcttcact taagtagact ggcgcccaat
    17641 caagtacacc ataacgcacg aaataaacaa atatatttgc aaaggcaatt gcccataccc
    17701 atttattgtt tagtacatat ttaaataaaa tttcttttgt agttaattct gtttctaatg
    17761 ttttcttatc gcttgtagca aagtcgtttt tataaatttc gattggaggt aaaccttgag
    17821 attgaggtgt gtctctaatc aatacgtatg aaattgcggc aatgataagt gctaagagtg
    17881 cagggtaaat gaatacacct tcgaaccctt ttaaataacc aaagttgata aatgctgttg
    17941 ttgtaatacc ccaagcagca ataggtgcca taatacctcc accaacatta tgcgcaacgt
    18001 tccaaagggc agtcttactt ccgcgttcac ttacactaaa ccagtgaacg agaacacggc
    18061 ctgaaggtgg ccagcccata ccttgaaacc atccatttaa gaataatagg acaaacataa
    18121 taccgatacc tgatgtaaag aacggtacaa atcccattaa caaattgacg atagcagtga
    18181 gtgctaatcc aagaactaag aatatccgag cattgctccg atcacttaca gtacccataa
    18241 agaacttact aaatccatat gcgatggaaa cagcagaaag tgcaaaacct agttccgctt
    18301 ttgtaaaacc ttgctcttgc aatgctggca tcgctaatga aaagttttta cgtaataaat
    18361 agtacccagc gtaaccgatg aaaataccaa gaaatacttg gagacgtaat cgtttatagg
    18421 tatcatctat ctgattttct ggcaaaggct taatatgctt tgcaggttta agaaaattca
    18481 taaaatcctc cttaatatgt atttatatgc attttgtgcg ataaacttat attagacatg
    18541 tatgaaagcg ttgtcaatat acttttgtga aatcttgcat atttagttat tgaagtattt
    18601 tataaacatt tagaaaaaat aaattgctac acataaattt ataattatgc aattattatg
    18661 tcgttataca tactagctgt gtatctacga atgagtacaa acatattttt atttgcagaa
    18721 aaggggtaaa tggcatataa ctatcttttt tatgtaagtt ggtataaaat ttatactaat
    18781 aggagggata gtatgaatat agtagggcat catcacatat ccatgtatac aaaagatgca
    18841 aaacgtaata aggattttta cacaaatgtc cttggattac gattagttga aaagtcggtt
    18901 aatcaagaca atccttcaat gtatcatttg ttttatgggg acgaagtagg tacagccgga
    18961 acaattttaa gcttttttga aattcccaat gcgggtcata agcagccagg tactgaaacg
    19021 atttatcgat tttctttatt agtaccaaat caagcggcac ttcattattt tgaaaaacgc
    19081 cttgataata atggtattac gtctgaacgt ttgtactatc ttggacaaga aggtgttgtc
    19141 tttaaagatg aagacgactt agaaatcata ttgcttgtta atgatagttt tgaagtacca
    19201 catcaatggc aacataacgt ttatagtgaa atacctcaag catatcaaat tttaggaata
    19261 gggccagtcg aattaagagt tagaaatgca gcgcgtacgg tagaattttt ggaaaatgtc
    19321 ttaggttatc gcaaaagaga taataaatca ttcgatgtgc tgacattagc accacaaggt
    19381 ttatattcgg attttgtagt tattgagcaa cagggacaac gtgaaagacc tggacgaggt
    19441 tatatccatc atattgcaat taatacacca caaatgagtg acttagatgc aatttacaag
    19501 aaattacaac aacaaacaca aagtaattca ggtataattg atcgctattt ctttaaatca
    19561 ttatactatc gccataattc aattatgtat gaatttgcga ctgaagcgcc tggatttact
    19621 attgatacac ctcttgaaca attaggaagt caattgaact tgcctgactt tttagaagca
    19681 gaacgtgaac aaattgaaag taagttacac gaaatataaa ggagaatgtt taatggcaaa
    19741 attagaaatg aataaaaata cacctcttga gtttggtttg tattccttag gtgatcattt
    19801 attgaatcca ttgaaaggtg aaaaagttag ttatgagcaa cgtattaatg aaattattga
    19861 agcaagtaaa ttagcagatg aagcaggtat tgatgttttt gcagttggtg aaagtcatca
    19921 ggagcatttt acaacacagg cacctacggt tgtgttaggt gcaattgccc aagcgacaaa
    19981 gcatatcaaa gtttcaagtt cttcaacgat tattagtgca acagatcctg taagagtatt
    20041 tgaagacttc gcgacattag atttgatttc tcatggtaga gccgaaattg tagctggcag
    20101 agcatcaaga acaggtattt ttgacttgtt tggctatgat ttaaaagact atgatgaatt
    20161 gtttgaagaa aaattaggtt tacttttaga gttaaataaa actgagcgta ttacttggtc
    20221 tggaaaatat cgtccagaac ttagaaatat gaaaatattc ccaagaccaa tcgataatac
    20281 attgccaata tggcgtgctg ttggtggtcc acctgcaagt gctattaaag cgggaaaaca
    20341 aggtgtgcca atgatgatta caacccttgg tggcccagca atgaacttta aaggttctat
    20401 agatgcttat cgtcaagcag caactgaagc aggtttcgat gcttcgccta agtctttacc
    20461 agtaagtata gcgagtctgt tttatacagc tgaaacaact cagggtgcta tgagagaatt
    20521 ttatccacat ttgaatacag ggatgtcatt tattcgtggc gttggttatc cgaaacagca
    20581 atttgctaat tcaccagatt atagagaagc gctaatggtt ggaagcccgc aacaaattat
    20641 tgaaaagata ttgtatcaac acgagttgta tggtcatcaa cgttttatgg cacagcttga
    20701 ttttggcggt gtgccatttg aaaatgttat gaagaatata gagttaattg gcaacgacat
    20761 tataccggcg attaaaaagc atttatcaaa ataggagggg cgtcatcatg aatattgtat
    20821 tattgtcagg ttccacagta ggttctaaaa cgagaattgc tatggatgat ttaaaaaatg
    20881 aactagaagt catcaatgag ggacatcaaa tagagttgat ggatttacga gaacttgaat
    20941 tagaatttag cgttggaaag aattatctag atactacagg agatgtatat aaattaacga
    21001 cgtcgttaat gcaggctgat gtgattttta ttggttttcc aatttttcaa gcttccatcc
    21061 ctggtgcttt gaaaaatgtg tttgatctac ttccagtcaa tgcgtttcgt gacaaggtaa
    21121 taggacttgt agcgacagca ggttctagta aacattattt aattcctgaa atgcatttaa
    21181 aaccaatatt gagttacatg aaagcacata cgatgcaaac gtatgtattt attgaagaga
    21241 aagatttttc aaatcaacaa attgtcaatg atgatgttgt atttcggtta aaagcgttgg
    21301 cacaatccac aatgcgaact gccaaagtac aacaacaagt gtttgaagaa gaaaacaacc
    21361 aatacgactt ttaaagtata aaaataagac gctcggcaca ctaaatttgc aagtgtttga
    21421 gcgtcttttc atattaacta tatagccaat gaacgacgat aaaggcaagt gatgacaagc
    21481 atattgaggt aataatgatt gtcataagcg gtttaagtgc gcgattttta agatctttaa
    21541 atgcaacatt taaccctaaa gcaaccatgg ccattaataa gcaaattgtt gatacagtat
    21601 ttaaaatatt tagcaatgct gacggaatag ttacatatgt attcactaag gccataatga
    21661 caaatccaat taaaaagtat ggaatgctta ttcgaccatt gctagatgat tctgatgaac
    21721 ggaaacgcat aattaaaata agtacgatgg ttaatggaat cagtaagaat actctgccaa
    21781 gtttaccaag aagtgcaatt ttaagtgcat cactaccacc aaagccacca gctaggacaa
    21841 cgtgtgcaat ttcatgaaga ctaacgccag accaagcgcc ataaacattt gtcgtcattg
    21901 aaaagatagc atagatagct gtatatataa gtgaaaatat cgtaccaatc aatgcgatga
    21961 taccgatact aatagctgta tccttttcac gtgatttgaa tattggagcg actgcagcaa
    22021 tagcggcagc accacaaacg cctgtaccta cacctagtaa taatgcgatg tttttgtcac
    22081 catgcaacag tttgttgaca aagagcatca ttacaatact aaaaataacg acacctacat
    22141 cgatggctaa tagtctacta ccttgaccga taatatcgaa tatattgagt ttaagtccat
    22201 ataggatgat tgcaaatctt aataaatatt tagatgaaaa cgtaatacct gagctatatt
    22261 gttcaggata tcctctaaag tgacgatata gaatagcgat taatatcgcg atagttaatg
    22321 cgccaacctt atctaagatt ggcaatttag cagctaaaaa gctaaataat gcgactataa
    22381 atgttaatgt tagtccaatc ataaaatgct tatttttcaa tgatgccatg agcagtgcct
    22441 cctttaatag cattttagca ctgttttgtc gtatttttaa atataaattt ggaatggtta
    22501 ataaagtaga gattaaatta agttgtgtga taggaaactt ggacatcaat caaagtaata
    22561 ggcactacaa cgcttattgg cggggcccca acaaagagaa tttcgaaaag aaattctaca
    22621 agcaatgcaa gttggggaag gacaacaaat ttaagataca aggcgtaata ttaatatgtt
    22681 attattatga taatttacaa aattatatga aaaataaatg agggtgtgat ggtatgtttg
    22741 gaatgaaagt gaatgaacaa ataatattaa aaattttaga agcccatgac acagaagcgc
    22801 ttttcaattt agtcaatcgt tcaagaaatt cacttaggga atggttacct tgggtagatg
    22861 caactgagca accatcagat acgcgtgcgt ttattaaaag aggacttttg caattttctg
    22921 atggtaatgg atttcagtgt ggcatttggt atgaaggaac gctagttggt gtcatcggtt
    22981 tacatgaaat taatcgcatg cacagaaaaa cttcattagg atattattta gataaacaat
    23041 ttgagggtca tgggattatg acacaagcag ttgaggcatt gataaagtat tgtttcgaag
    23101 agcttgactt aaaccgaatt gagattagtg ccgcagttaa taatgaaaaa agccgagcta
    23161 ttcctgaaag gctgggattt actagagaag gtatgttacg tgacaatgaa ttactaaatg
    23221 gtatttattc atcgagttac atctatagtt tattaaaatc agaatacgac caaaaatgac
    23281 aaattagact tacaaaagag tgatgacatt taaaatggca tcgctctttc atttaatttt
    23341 ttaaaataaa aggttgttga cagtattatt ttataacaat ataatgattt tgataattat
    23401 tatcaactag ataatgttca tgggaggatg ccttaaaatc atcattttaa gagccacgta
    23461 ttatcttggc atttagggag tgtgagatag tacgaatgag ggcatgtcta tggggataat
    23521 agaatttcta taatgaggtg tcaaaatgaa aaagttaaca acgctattat tagcatcaac
    23581 gttattaatt gctgcatgtg ggaacgacga tagtaagaag gatgattcaa agacatcgaa
    23641 aaaagatgat ggtgttaaag cagaattaaa acaagcaaca aaagcatatg ataaatatac
    23701 tgatgaacag ttaaatgaat ttttaaaagg tacagaaaaa tttgttaaag cgattgaaaa
    23761 taatgatatg gcccaagcaa aagcgttata tccaaaagtt cgtatgtatt atgaacgctc
    23821 tgaaccagtt gcagaagcat ttggagattt agatcctaaa attgatgcac gtcttgcaga
    23881 tatgaaagaa gagaaaaaag aaaaagaatg gtcaggatat cataagattg aaaaagcatt
    23941 atacgaagat aagaaaattg atgatgtgac taaaaaagat gcacaacaat tattgaaaga
    24001 tgcaaaagaa ttgcatgcca aagctgatac attagatatc acaccaaaat taatgttaca
    24061 aggttctgtt gacctattaa atgaagttgc aacttctaaa atcacaggtg aagaagaaat
    24121 ttattcacat acagatttat atgattttaa agcgaacatt gaaggtgcac aaaaaattta
    24181 tgacttattt aaacctattt tagagaaaaa agataaaaaa ttaagtgatg atattcaaat
    24241 gaacttcgat aaagtgaatc aattattgga taaatataaa gataacaacg gcggttatga
    24301 gtcatttgaa aaagtatcta agaaagaccg taaagcattt gcggatgctg ttaatgcatt
    24361 aggagagcca ctaagtaaaa tggctgtgat tactgaatga caaattatga acaagttaac
    24421 gatagtacgc aattttcaag acgtacattt ttgaaaatgt taggtattgg cggtgccggt
    24481 gttgcaattg gcgcaagtgg tgttggtagc atgtggtctt tcaaatcaat gttcaataca
    24541 ccagaagatc cggaaaaaga tgcgtatgaa ttttatggta aagtgcaacc aggcattacc
    24601 acacccacgc aaaaaacatg caatttcgtt gcgttagatt tgaagtcaaa agatagagat
    24661 gcaattaagg caatgtttaa aaagtggacg gttatggctg atcgtatgat ggatggtgat
    24721 aaagttggca agccgagtaa caatccttta atgccaccag tagataccgg tgaatcgata
    24781 ggattaggtg caagcaagtt aacgattacc tttgggatta gtaagtcttt gatgaagaaa
    24841 attgggttat ctagtaaaat tcccgatgcc tttaaagatt taccgcattt tccgaatgat
    24901 cagttaatag acgattacag cgatggtgat attatgattc aagcatgctc aaatgattcg
    24961 caagtatcct ttcatgcggt tcataattta gttcgtccat ttcgagatat tgttaaggta
    25021 cgttgggcgc aatctggttt tatctctgct aaaggtaagg aaacacctag aaatttaatg
    25081 gcatttaaag atggaacaat taatcctaga aagagtaatc aacttaaaga ttatgtgttt
    25141 attgatgacg gatgggcgaa acatggaact tattgtgttg tcagacgtat tcaaatacac
    25201 attgaaacgt gggatcgtac tgcactggaa gaacaagagg ctacatttgg tcggaaacga
    25261 catagtggtg cgccgttaac aggtgggaaa gagtttgatg aaattgactt aaaagcgaaa
    25321 gatagtcatg gcgagtatat tattgataaa gatgcccata cgagactagc gaaagaagca
    25381 aatacgtcaa ttttacgtag agcctttaat tatgttgatg gtacggatga ccgcacaggt
    25441 aacttcgaaa caggcttgtt gttcattgct tttcaaaaag cgacaaaaca atttatcgat
    25501 atacaaaata atttaggtag taatgataaa ttaaatgaat atattacaca tagaggttct
    25561 gcttcatttt tagtattacc aggtgttagt aagggaggat accttggtga aacattattt
    25621 gattaaattt gtagcaatgc taataactgc tgctatggtg tgtagctttg ggttactgaa
    25681 aagtcaggca gcaaaacaac aaagtattag tgatgtatat agtgtgataa cggatgcgaa
    25741 atctgcactt tctaataatt cgatatcgaa tgacaataag cagaaagcaa ttgagcaagt
    25801 ggtaagtgca gttaagaaat tatcgcttga agataatagc gaaggtaatg ctgtcaaatc
    25861 agatgtgaga aagcttgaag atgcaaaagc gaatgataat caaaaagata cactttcgca
    25921 attaacgaag tcattaattg cttatgaaga gaaattggct agtaaagatg cgggttctaa
    25981 aattaaacta ttgcaacagc aagtcgatgc caaagatgca gtaatgacaa aagcgattaa
    26041 agataaaaat aaagcagagc tagaatcgct gaacaatagt ttgaatcaga tttggacaag
    26101 taatgaaacg gtaattcgca attatgacgc aaatcaatat ggacaaattg aagtcgcatt
    26161 actgcaactt agaatcgcaa ttcataagtc gccattatat acagcgaaag tgtcacatgc
    26221 ttggacaact tttaaatcaa atattgatca tgtcgataag aaaagtgata cgtctgcaaa
    26281 agatcaatac catgtatcac aattaaatga tgagttagag aaggcgatta aagctatcga
    26341 cgacaatcaa ttgtcggatg ctgatgctgc gcttacacat tttatagaaa tttggccgta
    26401 tgttgaaggt caaattcaaa ctaaagacgg tgctttgtat acgaaaattg aagataaaat
    26461 accatattat caaagtgtat tagacgaaca taataaagca catgtgaaag atggtttagt
    26521 agatttaaat aaccaaatta aagaggttgt tggccatagt tatagctttg ttgatgtgat
    26581 gattatcttt ttacgtgaag gtctagaagt gttattaatt gtaatgacat tgactactat
    26641 gacgcgtaat gtcaaagata agaagggtac tgcaagtgtg attggtggtg caattgccgg
    26701 acttgtactg agtattatct tagcaattac gtttgtagaa actttaggta atagtggcat
    26761 tcttcgtgaa agtatggaag cgggattagg tattgttgcg gtcatattaa tgtttatcgt
    26821 tggtgtttgg atgcacaaac gttcaaatgc gaaacgctgg aatgatatga ttaaaaatat
    26881 gtatgccaat gcgattagta atggtaattt ggtattgtta gcgacgattg gtttaatatc
    26941 tgtgttgcgt gaaggtgttg aggttatcat tttctatatg gggatgatag gtgagctagc
    27001 gacaaaagat tttattattg gtattgcttt agctatcgtt atattaatta tctttgcgtt
    27061 attatttaga tttatcgtca gattgatacc tatattctat atatttagag tgttgtcgat
    27121 ttttattttt attatgggat tcaaaatgct tggcgtaagt attcaaaagt tacaattatt
    27181 aggtgctatg ccgagacatg tcattgaagg attcccaacg attaactggt taggattcta
    27241 tccaacttat gaaccattga tagcacaagc tgcctatatt atggtagttg ctatcttaat
    27301 ctttaaattt aaaaaataaa aaacaggccg agtgcctgtt tttttgttgc gatgttggaa
    27361 atattcggta ttgcagtata acgataatca cagcattaat tcttataaga ttaatgtgtt
    27421 ggcggtttgc ctcggtatgt aaatttaacg atgaacgtac tgaactcaaa gagcaatatg
    27481 agtggcaatg tgagtaatat atttaatgtt aaatcgggtg gtgcaatgat acttgctaat
    27541 acaaagcaag cgaaataaat atattttcga taatgcttca atgatgtggt atctataaga
    27601 ccgaattttg caagtcctat gaataatatt ggtaattgaa atagaatgcc aaatgtgaat
    27661 aaccaacgta taagttcaat caaatatgct ttaaagccaa tgacaggcga aatgtttaaa
    27721 gttaatgata attttaacgc gaattgaatg atcattggaa agccaacata aaatgcaaaa
    27781 gcgacaccaa aaataaataa aagtacgcta aaaaagctat atttataaat aaattggcgc
    27841 tcattattat gcaaaccagg cgcaacaaac gcccacaatt gataaaacat aaccggtgaa
    27901 atgagacaaa acgcgatgaa aaatataatc atcacgtata tttggatcat ttctgtgaat
    27961 gaaaatgcat gtaaggacac atgtgcacgg gtaatatacg ttatgaatgg tgtcatccac
    28021 caaaatgatg aaacatatac gacgatgacc gtaatgacga acgacaataa aatttttact
    28081 aaccgatggc gtagttcgct aaagtgaacc attaatgtat catcagtact attgctctcg
    28141 ctgttgcttc gattccttac tgggtgtatc gtgagactct ttatctaaat cttctgttgc
    28201 agatttaaat tcttttaaag tagaaccgat agcacgacca aattgtggta attttttcgg
    28261 accaaaaata attaaagcga taatgctaat gacgacaaga cttgttggac ctgtgatgcc
    28321 taaaataaaa gtgttagtta tcatgataat caacctcact cataagtagt atatagtatt
    28381 tactttatac cgaacgataa tgattatcaa caaattttat ataataatta aaatattgct
    28441 ataaacacaa cttattgagg gattaattct gaaataacag cttggtcttg caagtcgata
    28501 taagtacaag tgcccgcttg aatatcgata tgccatttat aaatgccagc ttcagccatt
    28561 tcatcacaaa atgtttcaaa atctgtttgt ccttgttgat gtctagttaa tacatcttga
    28621 acgatagatt gatttgattt ttgagcaaca ggaatcgtac ttttcacaga tgacgtaacg
    28681 atatcatctt ctgattgatg tacgtatgtt gcagtgccat cttgaatgtt gacgatattg
    28741 taagtcatcc ccatatcttt aaaagctttg aatagttttg gaaaatcaac accagtaaat
    28801 tgttgatgtg cttgttgaat tgcagataat gtaaatgcca taatagtctc ctttgaatcg
    28861 tttgatttag tgaaaatgcg tgcatcatac taaaattaga atgctgttaa ttatgcggga
    28921 aggtgtaacg aaagtaaaca attatgactt gtaggcgttg aggtgtttgt ttcaaaatgt
    28981 catacatagc tggataatca tattttttgt acatatttga caaatatagt tgtcaaaaag
    29041 acaaatatca tttacagtgt aattaaggag ttgatgcgat gtgcgtaatc gattgaaaga
    29101 attacgagca cgagatggct taaaccaaac gcagcttgcc aaactagcgg gtgtttcaag
    29161 acaaaccatt tcgctaattg agcgaaataa ctttatgcca tcggtattaa ctgcaataaa
    29221 gattgctcgt atcttcaatg aaacggtgga aactgttttt attattgagg aggatgaggt
    29281 atgaaaatac taagatatat cggatatctt ttactaggtg gacttgtagg gggtatcatt
    29341 ggtggaattt taggtaattt tgatggattg ggtattgaga acttgacgtt tgcgacatat
    29401 accaatgtcg ttgtaatatc gattgttgcg acgattatta tcatattggt agaagccatt
    29461 gttttgatga atcaaagacg tgcattgaag tataagcgac ttgtagatga agaggtagat
    29521 atcgatgcaa cagatcaata tgaattgctt gcgaatcgtt atgttttaaa tggaagtata
    29581 ttaagcgtta tacagacaat tattgccttt gtagtgttac taatttttgt ggtagggcaa
    29641 gctgaagcaa atgcaatgct attcttttta ataccatttt ttgctagtgc tattttcaat
    29701 acacaattta cactgtttaa tagaaaattt gatgacagaa tgccaaaaat tgcagataag
    29761 aattacactg aaaagcgatt ggaaatattg gatgaaggtg aacgccatat agaattaatt
    29821 gcattattta aaacatatgc gatcaactta tcaatattga tactagccat tatttttata
    29881 gggtcttatt caattgctac aggaattaat caaagcttta gtttgctact tatcattgct
    29941 attttcatat ataacgcctt tagctattta ttgaagagaa gacgttttta ttaaaattaa
    30001 acaaagagga gcatggatat gacaacattg ttaaacgtag atcgtgtgaa caaacaatac
    30061 aaagattcgg attttaaatt gcaagatgca tctttaacga tttctactaa tgagacagtt
    30121 ggattaattg ggaaaaatgg ctcaggtaaa tcgacattaa ttaatattct agtaggcaat
    30181 cgacataaag ataacggtag tattgcattt tttggagaag aacatgctgc ggatgatgtc
    30241 gaatataaag aacacatagg tgttgtgttt gatgatctga gagtacctaa taaattgact
    30301 attaaagata ttgataaagt atttcaatct atttatacga cttggaatag tcaaaaattc
    30361 tttgatttaa tcaaatattt cgagttacca cgacaaacta aaattaaaac tttttcaaga
    30421 gggatgcgaa tgaagatagc tttaacgatt gcgctttctc atgatgtgaa gctattaatc
    30481 ttagatgaag caactgcagg tatggatgtt tctggacgag aagaagtaat ggaactatta
    30541 gaagattttg tcgctcaagg tggaggcatc ttaatatcat cgcatatttc tgaagatata
    30601 gaacaattag cggataaatt agtgtttatg aaagatggac gaatgatttt aactgaacag
    30661 aaagatatac ttttagcaca atatggaatt gttacgacag gagataaaga tgttgaaatt
    30721 actaagcatt taatcattgc ttctagattg tcaaagggga aatatcaaat tttagttaaa
    30781 gattatgcag aaattgaaaa tgcagagcct ttaaaacaca ttgatgacgc tacgaaaatc
    30841 ataatgcgag gtgaagtata atgaaaggta tgttcctaag tagtttttat gcaacgagaa
    30901 agcaaacata tatttatttt atagtcgcta tcatagctgc gggatacttt gcagtattta
    30961 atccgttgat gagttcggca atggctgggg ttatgttaat cacacccatt actgataata
    31021 ttaaacatga aaaagactca agatggatgt attatgtatc tactttaccg gttaaacgta
    31081 gtgattatat taagtcatac tttgcctttt atttaatctt attcggtgca agtttaatga
    31141 ttggattagt tgtgactacg atcgtgaccc aaagtgtgat gattggtatt atgtcaggtt
    31201 taatgagttt tggtatcata ggggcatact ctatcatttt cccattgaca tttaaatttg
    31261 gcgctgaaaa ctctaatgtc attatgatag ttgcatctat cctactactt ttttctttcg
    31321 tggtcttttt ctttatatat ggtatggtta gcggtgcatc ggcattagaa tttgaaaaaa
    31381 ttagcactga aggatggtta gttgtcatag tatatgcggt cattggtatc gttataacaa
    31441 gtgtttctta tatattgtct attaaaattt ttaacaaaca agaactataa tcaatattct
    31501 tggtgtgaag ctagtcattg aaataatatt tagagcaggt gataaatcaa tacgattatc
    31561 atctgctctt tttggtgttg tggaatgaaa tgcgggatga taggttgagg tgacatctct
    31621 atatcatatc atttgttttt agaagattag gttgtgtggg aattgcatcc ttttatagtg
    31681 aggattaacg tgtatatatc attgagtatg ttgctgaaat ttatttgcgt gtatcaattg
    31741 atgatggtat atgccaatga tttcctcgga aatataaaaa ctcgatcatg tatcataact
    31801 gatgattgtc acaaaagtat aatgtgaagt tcgacacttt tggattcagt tcaaatactt
    31861 tgaccgaggt gaatactatt tattcatgtt tattacgtag acgttgattt ttaaaataaa
    31921 tcagcatgat gcagaaagtc gcaacggata ccgagataat cattacttgg tcatgcccct
    31981 taaagataag gctgatgata tatgatacta caagtatgcc tagtgaagaa gaaatatatt
    32041 ttgcgtttgt cagttcatta tggaaataag gcgtgattaa ccataatcca atatagaata
    32101 ttaaaacact gatatacatc atattaattt caaacaagtc atttagttta ttgttattac
    32161 taaaaacaat tgcagcatta atcacaccta aagcgatatt gattaataga tgcgtatacg
    32221 ataaacggaa accgatagat gacaatttat gattaatgta attttcagta atgatccaat
    32281 atacaccgaa aagactaatt aaaatcataa attggaatat ataaatgtaa ctaaaatgat
    32341 caatgctaaa tgatgacgaa gctaaaccaa ccagtacctc gccaaagata ataattgtta
    32401 gtaacgaaaa acgttctact aaatgcatca tattaacagg tgataataca agatatttct
    32461 gaaatggaat aagtcctgtc gctgcaatga atacgcctaa aaatccaggg atgtaatgga
    32521 tactttgtgg tagtactaat gatagaaatg ataaaaatga aatcacaaag gctacgctcg
    32581 caaaagcttg acatgtacgc ttatcgccat aatctaaccc tgtacgtata tgtaataaat
    32641 actgtaatcc gatacttaaa tacataattg ccacgcataa gaagaatggg aagaatgtct
    32701 tttcaaagtc cggatatagg ctgttagata ggaagaccat gatgaacata ttaaacatca
    32761 taaacgagac gtctttgaat gtaacttgac caaatcgatt tgtaaaaaat gtttgatgag
    32821 accacattaa ccataagaac aaactcatga cgatgtattt gaaaaataaa tcagctgaaa
    32881 tggaaccgtt ttgtgttgtt aaaatcacat gtgcaatttt ttgaatggca tagacgaaaa
    32941 ttaaatcaaa gaacaactca tggaatcctg cacgcttttc agctaaatgt tttggtgtta
    33001 atgcattaac cataaaattt taactccttt aagatgtgta attaatttac taagtatact
    33061 atttattttt tctagtgaat aggggcagat ttggcgatga agtggaagga gaggtgactg
    33121 catggtaatt gcggaattaa caatcatcag cgatttaata tttgactaga gacgtaatgg
    33181 taataaaaaa ttgatgagaa attgatggtg aaaccagctg tgaatagcga tgcaatgata
    33241 gatagaattt aattagagtc attacgcgaa atgattaatg ataatttgtg gtaaatcaaa
    33301 gcataatttt gtactataga tgaggatgat agagcatatt taagagtgtg aaatgttaaa
    33361 gtgaaaccgt ttgcgtttcc gattgcccaa acaaattaca tcattgtata atatgatttg
    33421 ttaaatgcat aacaagaatg aaaatgtaac atacgtagca attggtttca taaattggat
    33481 gttagtggcg tattggttca ttagacgtat tagtaataaa attgtatata tcataaggag
    33541 atgaatatga catgacgaga gtcgtattag cagcagcata caggacacct attggcgttt
    33601 ttggaggtgc gtttaaagac gtgccagcct atgatttagg tgcgacttta atagaacata
    33661 ttattaaaga gacgggtttg aatccaagtg agattgatga agttatcatc ggtaacgtac
    33721 tacaagcagg acaaggacaa aatccagcac gaattgctgc tatgaaaggt ggcttgccag
    33781 aaacagtacc tgcatttaca gtgaataaag tatgtggttc tgggttaaag tcgattcaat
    33841 tagcatatca atctattgtg actggtgaaa atgacatcgt gctagctggc ggtatggaga
    33901 atatgtctca gtcaccaatg cttgtcaaca acagtcgctt cggttttaaa atgggacatc
    33961 aatcaatggt tgatagcatg gtatatgatg gtttaacaga tgtatttaat caatatcata
    34021 tgggtattac tgctgaaaat ttagtggagc aatatggtat ttcaagagaa gaacaagata
    34081 catttgctgt aaactcacaa caaaaagcag tacgtgcaca gcaaaatggt gaatttgata
    34141 gtgaaatagt tccagtatcg attcctcaac gtaaaggtga accaatctta gtcactaagg
    34201 atgaaggtgt acgtgaaaat gtatcagtcg aaaaattaag tcgcttaaga ccagctttca
    34261 aaaaagacgg gacagttaca gcaggtaatg catcaggaat caatgatggt gctgcgatga
    34321 tgttagtcat gtcagaagac aaagctaaag aattaaatat cgaaccattg gcagtgcttg
    34381 atggctttgg aagtcatggt gtagatcctt ctattatggg tattgcacca gttggcgctg
    34441 tagaaaaggc tttgaaacgt agtaaaaaag aattaagcga tattgatgta tttgaattaa
    34501 atgaagcatt tgcagcacaa tcattagctg ttgatcgtga attaaaatta cctcctgaaa
    34561 aggtgaatgt taaaggtggc gctattgcat taggacaccc tattggtgca tctggtgcta
    34621 gagttttagt gacattattg catcaactga atgatgaagt tgaaactggt ttaacatcat
    34681 tgtgtattgg tggcggtcaa gctatcgctg cagttgtatc aaagtataaa taataagaaa
    34741 acaggttatc acaacagtat taattacatg ttgtcataac ctgtttttat ttgtttatgg
    34801 atttattggg taatattagt catttgatgg tttaattgca aatgctctaa cagggaaccc
    34861 aggtgcatct tttggtttag ggctgatagc gtaaatgatg gcgccacgag ttggtaattg
    34921 atctaaatta gttaataact cgacttggta tttatcctga ccaagaatat aacgttcgcc
    34981 aactaaatca ccatttttta caacgtccac agatgcatcg gtatcgaatg tttcatgacc
    35041 aacagcttca acacgacgtt cttcaattaa gtacttcaaa gcatctaatc cccaacccgg
    35101 tgcatgttgt tgtccgttcg catctttgtt ttcaaacttt tcaatattag gccaacgttt
    35161 tgaccaatcg gtacgaagtg caacaaaagt gccaggttca atagtaccat gctctttttc
    35221 ccatgcttct atatgcgcac gtgttacaat gaaatcattg ttgttcgcta cttctgttga
    35281 aaagtctaat acaattaacg gcaataccaa ttcttttaaa tcaatgtctt ctaaataacg
    35341 tttattctcg acaaagtgaa ttggtgcatc aatgtgagta ccatattgcg ttacaatatt
    35401 ccaacgttgc acatagaaac catgatcttt aaccgtgaat aaagttgaaa cttcgccttt
    35461 ttcaaactca ctaaaacgtg gtatttccgg atcaaatgta tgcgttaaat caacccaagt
    35521 tgcttgtttt aaagtattta attgttgcca taaaggatat tgtgtcataa aatcacccgt
    35581 ttttagttta ttatatgata aatgctgcga ttattcttgg cgtttagctt taacagcatt
    35641 cacaagcaca gtcaatgcat ctttaacttc ttcttctttt cgcgttttca aaccacagtc
    35701 agggtttacc cagaataatg agcggtcgat ttgttgtagt gaacgattga ttgctgtagt
    35761 aatttcttct tttgttggaa tacgtggact atgaatatca tacacaccta gaccaatacc
    35821 taaatcataa ttaatatctt caaagtcttt aattaaatca ccatggctac gagatgtttc
    35881 aattgaaata acatcagcat ctaagtcatg aatagcatga atgatttgac cgaattgaga
    35941 ataacacata tgtgtatgga tttgagtttc atcacgaact gaagacgttg caagtttaaa
    36001 tgataaaaca gcatctttaa gatattgttc gtgatattca gagcgtaatg gtaagccttc
    36061 acgtaatgca ggttcgtcaa cttggataac tttgattcct gcagcttcaa gtgctaatac
    36121 ttcttcgttg attgctaaag caatttgatc ttgaacgact ttacgtggta aatcaacacg
    36181 ttcaaatgac cagtttagaa ttgttacagg tccagttaac atacctttaa ctggtttatc
    36241 tgttaagctt tgtgcataaa ctgtttcatc aacagttaaa ggcgctgtcc attttacatc
    36301 accataaatg attggtggtt ttacggcacg tgaaccatat gattgcaccc aaccgaattt
    36361 agttactaag aaaccttgta atttttctcc gaagaattca accatgtcat tacgttcaaa
    36421 ttcaccgtga actaatacat ctaagccaat gtcttcttga attttaatcc atcgagcaat
    36481 ttcatttttt aagaatgttt catatgcttc gtctgtaatg cgtttgttct tccaatctgc
    36541 acggtatttt cgaacttctc ggctttgtgg gaatgatcca atagttgttg ttggtaaatc
    36601 cggtaagttc aaacgttttt gttgttgttc aatacgttgc gcgaatggtg attgtcttga
    36661 agtacgcacg ctttcgaaat cataatctaa gtttttgaat gattgatttt ggaaacgctc
    36721 ataacgtgct tttaatttat catatttaac actatcgttt tgattaaata ggcgacgcaa
    36781 tgcatctaat tcgtctaatt tttcagttgc aaagcttaag ccttcgccaa cacttgtatc
    36841 taatgtttca tcatctaaag atactggaac atgtaataat gaagatgatg gttgaatgac
    36901 aagttcatta gtgtgtgcta acaatttatc gattaagact tttttagctt caatgtcact
    36961 tgcccacaca ttacgaccat caataattcc agcgtataat gtttttgatt tatcaaaatc
    37021 tccagcttca atttgtttaa ggttatagcc attatcatgg acaaagtcta aacctaaacc
    37081 accaacaggt aaagaactta agaatttaag atgtgcacgt tcaaagtatg tttgaatgac
    37141 taatttttta gcaacaccag ctttttcgaa atagtcataa gcttcacgtg taatattttc
    37201 atagctttcg ctgtcgtctg taactaagat tggctcatca acttgaatgt actcagcacc
    37261 tgcatcaatt aatgattcaa acacttcttt ataaagtggt aataacgttt taactttttc
    37321 ttcaaaagtt tggtgaccgc cttttgataa tttaacaaaa gtaatcggac caacaatgac
    37381 agggtgagcg ttaacgttta aagattgggc gtatttaaag cgatctaata atacattgcg
    37441 actcacttta ggctcaacat tgtcccattc aggtacgatg taatgatagt tagtgttaaa
    37501 ccattttata agtgcacttg caacatggtc tttattaccg cgagcaatat caaataataa
    37561 gtcatcatcg atagttctac cttggaaacg ttctgggatg atgttgaata ataatgacgt
    37621 atctaaaata tggtcatata aagagaaatc accaactggg atgctatcta agtgatagta
    37681 cttttgtaat aataaatttt ctttatgtag atcagttaat gtttgatcta attcttcttt
    37741 agaaatcttt tttgcccaat aactttcgat ggcttttttc cattctcttt ttctacctaa
    37801 tcttgggaat cctaagtttg atgttttaat tgttgtcata atattgcctc cttgtgagca
    37861 gtaatagatt ttgagtatgc tgcaagttct aatgaatctt cgacattttg aaacggtgtg
    37921 ataatgtata aaccattaaa atattcatga acagtatcga ttaaatcctt tgaaagctta
    37981 agacttagct ctcgtgtttt ggcgttatca tctttaactg cttcaaattg ttgtaaaatt
    38041 tcatctgaca tcttgattcc tggcacttca ttatgcaaaa agagtgcgtt tttgtaactt
    38101 gcgataggca taatgcctat gaaaaatggt ttgttcaagt gcttagtggc atggtaaatt
    38161 tcaatgattt tctctttgct gtacacgggt tgtgttataa aataagacat tccgctttct
    38221 atctttttct ctaatctttt gacggcacca tctaattttc gaacattagg attaaaggcg
    38281 ccagcgatgt tgaagtgtgt acgtttcttc agcgcatcac cgtcagtgtt aataccttga
    38341 ttaaatctta gagcgagttc agttaatcct ttagaattaa catcatagac attggttgca
    38401 cctggtaagt gaccaacttt tgaaggatca ccagttatgg ctaatatttc gttaacgcca
    38461 atgagcgata atccaagtaa atgggactgc aagccgatta agtttcggtc tcgacatgta
    38521 atatgtacga gtggttcaat attgtaatat tgtttaatta agcttgcagc agcaatgttg
    38581 ctaattctga cagttgccaa tgaattatct gcgagtgtta ccgcatctac attagctttg
    38641 tcaagtttag cgatattttc aaaaaatcta tccgtgtcta aatgtttcgg tgtatccaat
    38701 tcgataataa cggttggacg ttcttgaacc ttagatgtta atgattgtct aactttattt
    38761 tgagatggat tgaaaagtgc tttcgttggt atcggaatca cttttttgtc attaacaggt
    38821 ttaagtgtct gaatagattc tttaataaat ttgatgtgct ctggcgttgt accacagcaa
    38881 ccaccaatta aacgaacacc ttcgtgaatt aaattttgag caacttggcc gaaatattgt
    38941 gcattgtcac tatacttaaa ttcactattt tcaatatcta ataagctggc atttggatag
    39001 caagataagt atgcgtgctc tggtaattca atatgtgtga aagactcttg catatggtgc
    39061 gggccatgat gacaattgag tcccacgatg ttggcaccac attgaacgag ttgttttaat
    39121 ccttcattga ttgcctgacc attaactaag taatttgtgt ttgaagcggt taattgagca
    39181 atgattggaa tgtcgtattt ctttctcgtt cgtgaaatga catttgttaa ctcttctagg
    39241 tcgtaatacg tttcgaaaag tagcgcgtca acgccttctt caattaaggt gtctatttga
    39301 atttcagtat gataaagaat agtttgtaag ctgatatcct cttgtttgat acctctaaac
    39361 ccaccaactg tgcctaatat atacgtatct ttatttgctg ctttttttgc gatgcgtacg
    39421 gcggcttgat gtatagcttt aactttatct tcaagaccga atcgttttaa cttttcaaaa
    39481 tttgcaccat aagtattggt ttgaatgaca tcagcaccag cttcgatata tgaacgatgg
    39541 atacgttcaa ctttatctgg atggctaaga ttatatgctt ctggacaggt gtctaatcct
    39601 tcagagtata aaatggttcc aatagcgcca tcagctacta aaacattatc tttcaattgt
    39661 gtgaggaatt gactcattga atgcctcctt taatgcgtat ttgatgtctg caatgagttc
    39721 atcaggatct tcgagaccaa cacttaatcg gaatagaccg aaagtgatac cacgttcttg
    39781 tctcacttct tcaggtagtg cagcgtgaga cattgttgct ggatgtgaaa ggatcgtttc
    39841 aacaccgccc agactcactg aaacgagtgg taatgtcagt gcatcgacaa attgttgtgc
    39901 tttagactca tcagctaaac gaaagccaat aacggcaccg ccatttttag cttgttctaa
    39961 atgagcagta gtgagtcccg gataataaac ttctgaaatt tcatcttgct ttattaaaaa
    40021 tgacacgatt ttttgcgcgt tttcgacaga ttgtttaaat ctgattggaa atgtttttaa
    40081 atgtttagca agtgtccagc tatcttgagc agataacata ttgcctgtac cattttgtat
    40141 taaataaaga gcttcactaa ttgcctcatt attagtaatg acagcaccag caattaaatc
    40201 gctatgtcca cttaaaaatt ttgtagcact atgaatgaca atatcagcgc caagtaataa
    40261 aggtgattgg cctaacggtg tcataaatgt attgtccaca gctaccagta gttcatgctt
    40321 ttcggctatt ttagaaacag ctttgatatc agtaatttta aaacagggat tcgatggtgt
    40381 ttcgatataa attaattttg tgtttgattg aatggcaccc tcgatttgtt cgagcttggt
    40441 agtatctacg gttgtaaatt caatattaaa tcgattcaaa atttgctcag tgaggcgaaa
    40501 agtaccgcca tatacatcat cgggtaagat gacatgatca ccagatttga aagtcaaaag
    40561 tactgctgaa atagcagcaa tacctgatgc aaaagcaaaa gcgaattttc cctgttctaa
    40621 tcgtgctaac ttctcttcta aaagttcacg gttagggttg ccacttcgtg cataatcata
    40681 tttaacatcg ccaccaagac ttgtttgatg gaatgttgaa gaatcataga gtggtgggtt
    40741 agctgaatga tattccacac ctctacgcca atcgaatatc acttctgtct cttttgaaag
    40801 tgtcatacaa tctctccaat ctgagcttta tctaatgctt ggatgatatc gcgttcgatg
    40861 tcttcataat tttcaacacc tagtgataag cggattaaat actcatcaat gccacgttta
    40921 tctttttcag catctggcat atcaacatgt gtttgggtgt aagggaaggt cactaatgtt
    40981 tcagtacctc ctaaactttc tgcaaaaatg caaatgtcta aattttctaa taatttagcg
    41041 acgctatagg ccttgttaag tcttaaacta agcatgccag tttgcccgct atatagtact
    41101 tcgtcaattg cttgaagtga ctgacatttt ttagcaagtt ttctagcgtt tgattgcgca
    41161 cgctcaatgc gtaaatgcaa agttttaagt ccacgtaaca acaaataact atctattggt
    41221 gaaagtgttg cgccagtcat gttgtgaaaa tcaaacaact gttgcgcgag tgattcatct
    41281 ttgacggtta cgacacctgc tagtacatcg ttatgtccgc caatatattt cgtggctgaa
    41341 tgtaagacta tatcagcacc ttctgctagt ggtgttgaaa gataaggtgt taaaaaagta
    41401 ttgtcgataa ttgacaataa gcctttagct ttacaaagtt gatagtatgg ctttacatca
    41461 atagcaatca tttgtgggtt agatattggt tcaatgaata atgcaactgt tttatcagtg
    41521 atttcttttt caacttgttc ataatctgta aaatcaacgt acttaaattt gatattgtat
    41581 tgttgctcat aaaattcaaa taatctaaat gtgccgccgt ataaatcgaa tgaaactaaa
    41641 atttcatcgt gtggtttaaa taaattacat attaattgaa tggctgacat tccacttgat
    41701 gtagcgaatg atgcaatacc atgttcgagt ttggcaaaac aggtttcaaa tgttgagcgt
    41761 gtaggatttt tagtacgtgt ataatcaaaa cctgtcgatt gtcctagttt tggatgcttg
    41821 taggcagtag ataaatggat tggattcgct atagcaccgg ttgaatcatc ggttaatgtg
    41881 atttgggcta actgtgtatc cttcatatta agactctcct ataagaaaaa ataaaaaaag
    41941 cttccgtcct tcgtacccga atgaatcgga taaaaaggac gaaagcttat gtttcgcggt
    42001 accaccttta tttgttattc catcgctgaa ataaccttat tcagtacgca ttaaaagtaa
    42061 atatgcttac tgaacaatta tcacaattaa agtcagtaag taaggatata gtaatgtgct
    42121 atcccatact tattaacaaa aaatcgtgcg taaagaatcc agtacgccat ttaacatcaa
    42181 tgttaatact gtatcgctat aacgggcgaa cccgtagaca cctcatattg gcatcaacac
    42241 tccaaggcca ttttcaaaca cgctttcaaa atcttctctc agctactaaa gactctctgt
    42301 ataagcaggg tgtgttttac tttcctcttt attgtgttta cgtttcatta aactgttata
    42361 agatattaat tagcttacag agtaaaaaaa gatttgtcaa caattattca gaaaattttg
    42421 atttaaaagt taatttgttt gtgaaattgt aattggtatc ttgaagttga aaaatgaatt
    42481 attttttaaa taaagtgtgg ttaatggttg tctgactcat ttagaataca taaaatatat
    42541 ttaactgttg ttatcaaata aaaagtgatg tgagtgaatt gtcaaaaagt gaagatcaac
    42601 gtattactaa aacaaaagat gaacaaatta agcaaataga tatatcggat atcaaaccga
    42661 atccgtatca gccccgaaaa actttcgatg aaaatcattt aaatgatttg gcagattcaa
    42721 ttaagcaata tggaattttg caaccaattg tgcttagaaa aacagttcaa ggttattaca
    42781 ttgtagttgg tgaaagaagg tttagagctt cgaaaattgc tggtctaaaa tacgtatcag
    42841 cgattatcaa agatttaaca gatgaagata tgatggaact ggcggtcatc gaaaatttac
    42901 aacgagaaga cttaaatgcg attgaagaag ctgaaagtta tcaacgtttg atgacagatt
    42961 tgaaaattac acaacaagaa gtagcgaagc gattgagtaa gtcgcgcccg tatatagcga
    43021 atatgttgag gttattacat ttgccgaaaa agattgctga catggtaaaa gatgggcgac
    43081 tgacaagtgc acatggacga acgttattgg caattaaaga tgaacaacaa atgcttaggt
    43141 tagcgaaacg ggttgttaaa gaaaagtgga gtgttagata tttagaaaac catgttaatg
    43201 aattaaaaaa tgtttcgtca aagtcggaaa cagacaaagt agatataact aagcctaaat
    43261 ttataaagca acaagaacga cagttgcgag aacagtatgg taccaaagta gatatatcaa
    43321 taaaaaaatc ggttggtaaa atctcatttg agtttgattc acaagaagat tttctaagaa
    43381 taattgaaca attaaatcgt aggtatggta aatagttaca caattttata taataactct
    43441 ttgtgcaagt gtaaataaat tgtaatcagt gaacatttga ttctagatat attgagactt
    43501 tcgtaggttg gaagtatata aaaatatgaa tacaatattg atatatcata tagaagggag
    43561 taacgcttat catgaatcaa gtcatgaata ttatttcatc tctatttgag ccattaacaa
    43621 aaatagaaac atatgaaaac attgcaacta aaatcgctat gattgttatt tatattatcg
    43681 tagccctcat agttattaaa atactgaata aaatgattga acagggattt aagattcaaa
    43741 ataagagtaa aaagagtaac aaaaagcgct ctaaaacttt aatatctctt gttcaaaatg
    43801 tagtgaagta tatcgtttgg tttatagtta ttacgacgat tttaagtaaa tttggcatta
    43861 gtgttgaagg tgttattgca agtgctggtg tcgtaggttt agcagtaggt tttggtgctc
    43921 aaaccattgt taaagacgtt attactggat ttttcattat atttgaaagt caatttgatg
    43981 taggtgatta tgttaaaata aacaatggtg gtacgactgt ggcagaggga acggttaaat
    44041 caataggact tcgttcaaca cgaatcaata caatttcagg agaattaaca attttaccaa
    44101 atagtagtat gggtgaaata acgaactact caattacaaa tggtacagct atcgttaaaa
    44161 ttccagtgtc tgtcgaagaa aacattgata atgttgataa aaaactaaac aaactattta
    44221 cttctttacg tagtaaatat tacttatttg ttagtgatcc ggttgttatt ggtattgatg
    44281 ctattgaaga tacaagagta atattgagaa tatctgcaga aacaattcca ggtgaaggat
    44341 ttgctggagc tcgaattatt cgcaaagaag tacaaaaaat gtttttacaa gaaggtatta
    44401 aaacacctca accaattatg actgcttata atcatagtga aaacggtgtt tagtagttta
    44461 taatacatgg aggtcatatt taatggcgtc aaaatatgga ataaatgata tagtagaaat
    44521 gaaaaaacaa catgcgtgtg gaacaaaccg ttttaagatt attagaatgg gtgcagacat
    44581 aagaattaaa tgtgaaaatt gtcaaagaag tattatgatt ccacgtcaaa cgtttgataa
    44641 aaaacttaaa aaaatcatcg aatctcatga tgatacacaa agataggaga atgattaatg
    44701 gctttaacag caggtatcgt tggattgcca aacgttggta aatcaacatt atttaatgca
    44761 ataacaaaag caggtgcttt agcagcgaac tatccattcg ctacgattga tcctaatgta
    44821 gggatagtag aagtgccaga tgctagatta cttaaattag aagaaatggt tcaacctaaa
    44881 aagacattgc cgactacatt tgaatttaca gatatcgctg gtattgtgaa aggtgcttca
    44941 aagggagaag ggttaggtaa taaattctta tcacatatta gagaagtaga tgcgatttgt
    45001 caggtcgttc gtgcatttga tgatgataac gtaactcatg ttgctggtcg agtagaccct
    45061 attgatgata ttgaagttat taatatggaa ttagtactag cggacttaga atctgttgag
    45121 aaacgtttgc ctagaattga aaaattagca cgtcaaaaag ataagactgc tgaaatggaa
    45181 gtacgtattt taacaactat taaagaagct ttagaaaatg gtaaacccgc tcgtagtatt
    45241 gactttaatg aagaagatca aaaatgggtg aatcaagcgc aattactgac ttctaaaaaa
    45301 atgctttata tcgctaatgt tggtgaagat gaaattggtg atgatgataa tgataaagta
    45361 aaagcgattc gtgaatatgc agcgcaagaa gactctgaag tgattgttat tagtgcaaaa
    45421 attgaagaag aaattgctac attagatgat gaagataaag aaatgttctt agaagattta
    45481 ggtatcgaag aaccaggatt agatcgatta attagaacaa cttatgaatt attaggatta
    45541 tcaacatatt ttactgctgg tgtgcaagaa gtacgtgctt ggacatttaa acaaggtatg
    45601 actgcacctc aatgtgctgg tatcattcat actgattttg aacgtggatt tatccgtgcc
    45661 gaagtaacaa gttatgacga ctatgtacaa tatggtggcg aaagtggcgc taaagaagcg
    45721 ggcagacaac gattagaagg taaagaatat attatgcaag atggtgatat cgttcatttc
    45781 agatttaatg tataaacgat agagtgaagt taattaaata gtatatatgg agaagaggcg
    45841 gaatcaattg ttcgcctctt ttaattatgc gtataattta ttaaaagaat ggaataattt
    45901 tactcgcgtt aataatatct tgagtgctga aaaattgttt gccttcgcca gtataagcag
    45961 gctctaaaac aagattagcc tttgcacaat aaagccattc aggatgaatg ccactattaa
    46021 gtatctcttg gaattcttga aaatctttag accaatcaat atttaaattc attccttacc
    46081 acctcacaac tattatagaa cacgtgttcg cttttgtgaa gtgtattttt aaaaatatca
    46141 tagaaaattt caaatgaatt aatgtcaaaa atgatatgaa gagtagtttg aattattatt
    46201 gtaaaaagta atggcgcatg atataattct ttattgtgag taatgaaaat tattccttgc
    46261 ttatctgttt taagattgat aagccgtata gaccacaagg aggtgcaaat ataaaatgag
    46321 aacatatgaa gttatgtaca tcgtacgccc aaacattgag gaagatgcta aaaaagcgtt
    46381 agttgaacgt ttcaacggca tcttagctac tgaaggtgca gaagttttag aagcaaaaga
    46441 ctggggtaaa cgtcgcctag cttatgaaat caatgatttc aaagatggct tctacaacat
    46501 cgtacgtgtt aaatctgata acaacaaagc tactgacgaa ttccaacgtc tagctaaaat
    46561 cagtgacgat atcattcgtt acatggttat tcgtgaagac gaagacaagt aataattaga
    46621 gggggcgttt aaatgctaaa tagagttgta ttagtaggtc gtttaacgaa agatccggaa
    46681 tacagaacca ctccctcagg tgtgagtgta gcgacattca ctcttgcagt aaatcgtacg
    46741 ttcacgaatg ctcaagggga gcgcgaagca gattttatta actgtgttgt ttttagaaga
    46801 caagcagata atgtaaataa ctatttatct aaaggtagtt tagctggtgt agatggtcgc
    46861 ttacaatccc gtaattatga aaatcaagaa ggtcgtcgtg tgtttgttac tgaagttgtg
    46921 tgtgatagtg ttcaattcct tgaacctaaa aatgcgcaac aaaatggtgg ccaacgtcaa
    46981 caaaatgaat tccaagatta cggtcaagga ttcggtggtc aacaatcagg acaaaacaat
    47041 tcgtacaata attcatcaaa cacgaaacaa tctgataatc catttgcaaa tgcaaacgga
    47101 ccgattgata taagtgatga tgacttacca ttctaataaa aattaacgaa attaaagcga
    47161 aaaaattatc aaaggaggca cacaatcatg gcaggtggac caagaagagg cggacgtcgt
    47221 cgtaaaaaag tatgctattt cacagcaaat ggtattacac atatcgacta caaagacact
    47281 gaattattaa aacgttttat ctcagaacgc ggtaaaattt taccacgtcg tgtaactggt
    47341 acttcagcta aatatcaacg tatgttgact acagctatca aacgttctcg tcatatggca
    47401 ttattaccat atgttaaaga agaacaataa tatataattt attgtcaaac cccgtaggca
    47461 taggcttacg gggctttttg tgttttgggg tatagaaaaa gggcaaaaaa ggatgatgtg
    47521 aatgttttgt gttcggaatt tgcacaaaga tatgtttata ttgcaaaaat aatatgaatt
    47581 tagatgcata aaaaaagaac tacgcatttt aaataaaatg catagctctt ctttttcttg
    47641 catacgaatt aaaataactc gcgagaccta taagtctctt tcctcactag atagtttata
    47701 cttttggtct gttgaagtca ataattttat ctaaagctat aaaaaatctt ttgatagcta
    47761 atgcattatt ataatagctt tcgtttcttt tatatcgctt ttgaagttgg tccaaatcgt
    47821 gatatcttgc ttggataatt gcattactac aaacttgatt atgtaattct agcgtagcga
    47881 aagtatctat gaaatttttt attccgaaca tgtttctaga tatgcctata ttattccctt
    47941 tttcaaataa atattgagga gatctactgt cataatttag atttgctatg atgggttggt
    48001 tatgagctga tttgtttctt atatttttaa ctaaaggcat taaaatatta gcaactctca
    48061 attcttcgtc attgtacttc ttgtaataga agttgagaaa cgaaacgaat tgacctagtt
    48121 gcatgaattc aatgcaaacc catgcgggtg gattttgata gtatttattc aacttctcgg
    48181 gtagttgtcc tcgtttattc atatgcttga atatttcgtt tttatttttg attttggttt
    48241 ccataacttc ttctggtgtt cttgaatttg tgtcaaaatt tgaattgcta tatgatttat
    48301 caatacataa gaactcatct attattttat aaccatcttc ttggttattt tctgttatta
    48361 gttttaagac tagacacttt aaactatgtt caatatctaa agttaaatgc aacattgtgt
    48421 atcttaattt catatctata gttgctaaat ctgataaata agcaaattct atgaaatagc
    48481 cgccattctt tttttcgaaa ttttttcgga aataagctag tttgaagaag tgattatttt
    48541 ttctaagaat ttcatttgct ttttcggtgt caataatatt aaaaaatata ttcatctgtt
    48601 ttaatttcgc tatttgctca tcaaaattga gcataggctt aatttctgct agtgtatcat
    48661 ctttttccaa ttttaactcc ccaatcattc aaatttattc atcatatctt tcgccatctg
    48721 attagtaaca tgtgtgtata tctctagatt tttttataat ctgaatgacc tacatgctct
    48781 tgcattgctt ttaagttaat tcctaattga gcaagtgtag atatatgcga atgatgtaat
    48841 gtatgcgtcg ttacaggttt cttaatagaa ctaatctcag ttgtctcttt gatagtgccc
    48901 cctttaataa tgttgctaat tttgttcgag tcgatagggc caccagctgc atttgtgaat
    48961 atgtacccta tattaataaa cttatcatta caagcataag tgttcttagt aagtcgatgc
    49021 tatggatagt gaggcctata gccctatagc tgttacttgt tctagttgtc tcttttactc
    49081 cggatgctcc cgtcattttt cagttatcca attaacttta ccgtcgatat ctagcgtttt
    49141 atcttcatag tttatattta ctctctttat tgcaagtagc tcaccgatac gcatgtcatt
    49201 agcaatttga aactgtacca tagcttttac cattttataa ttacgttttg tcgttggata
    49261 tttttatact taattacata gtcgaaacaa tccagtaact ccttatcttc attatcttct
    49321 aaagtgttat tacgtttagc tagtaacgca tcactatagg gatatctatt ttatctatta
    49381 cacatataac tttgaattgc ttactatttt aaattaacaa attctattct cttagattta
    49441 gtccaattat gtgtagacga tttatagtta ttaaattcag agtggtagca aattaaagtt
    49501 aatcaagagt taagatgaat ttaattcatg agcacgtcta ttatttttat aattgaagaa
    49561 aataaagttt tacttcaagg aggtaattaa atatgttcaa aaaaaatgac tcgaaaaatt
    49621 caattctatt aaaatctatt ctatcgctag gtatcatcta tgggggagca tttggaatat
    49681 atccaaaagc agacgcgtca acacaaaatt ccccaagtgt acaagataaa caattccaaa
    49741 aagttgaaga agtaccaaat aattcagaaa aagctttggt taaaaaactt tacgatagat
    49801 acagccaaaa tacaataaac ggaaaatcta ataaatctag gaattgggtt tattcagaga
    49861 gacctttaaa tgaaaaccaa gttcgtataa atttagaagg aacatacaga gttgctgata
    49921 gagtatatac acctaagaga aatattactc ttaataaaga agttgtcact ttaaaggaat
    49981 tggatcatat cataagattt gctcatattt cttatggctt atatatggga gaacatttgc
    50041 ctaaaggtaa catcgtcata aatacaaaag atggcggtaa atatacatta gagtcgcata
    50101 aagagctaca aaaagatagg gaaaatgtaa aaattaatac agccgatata aaaaatgtaa
    50161 ctttcaaact tgtgaaaagt gttaatgaca ttgaacaagt ttgaaattaa gttgaattag
    50221 tatatacagt gttttattgc taatactttg aaagttaggt atctaaaggt gcctaacttt
    50281 ctttgttatg attagcacca tcatatagaa aattctttaa tgacgtttat gccgaaatct
    50341 acaaaaataa tttctctttt atggttaatc aatatgtttt tacattcgac aaccttaaaa
    50401 ttaacttctt tcaattccat aataaagtct ctataaaata acttagttta aaaacgattc
    50461 atatctttca gattcaaata ccatcatttt ctactaatac ttacacttta attacaatta
    50521 tgtaagttgt tttcagagac atcctatttt atattttgtc agaggtgatc ttttgaaaac
    50581 gattttaaaa acaataacat atctagcact tactatcatt ggcgcttatg ctgctttatt
    50641 cattttaaaa acaatagact ctcatggtat aacagatcaa tttaacccat tagtaaagga
    50701 agatgtttct tatgttaaaa cgactgaggt gtctactaga atggatgatc aactccgaag
    50761 ttatagtcaa agtgctttta ataaagaagg gaaagagacg caattaatgt atactgctac
    50821 atttgatgtt aaaccgcata gatacttgaa aattacacat aaaggtcatc acgtagaaac
    50881 ttttgaagaa gttgaaaagg ggcaagtacc taaaaaaagc attagacaaa ttgagtcgat
    50941 tatagcgtgc ttatattaca tggttcaatt ataaaatgag tacgaacgaa agtaacacat
    51001 gatgattaat ttaaaaatat ttgtaataat tatgaataaa attaaaaaca aggggtaata
    51061 caatctatat agcatataag cttttgttat gagtttcaaa aaaggaagag agagtgatat
    51121 tatgaaatta aaatcattag cagtgttatc aatgtcagcg gtggtgctta ctgcatgtgg
    51181 caatgatact ccaaaagatg aaacaaaatc aacagagtca aatactaatc aagacactaa
    51241 tacaacaaaa gatgttattg cattaaaaga tgttaaaaca agcccagaag atgctgtgaa
    51301 aaaagctgaa gaaacctaca aaggccaaaa gttgaaagga atttcatttg aaaattctaa
    51361 tggtgaatgg gcttataaag tgacgcaaca aaaatctggt gaagagtcag aagtacttgt
    51421 tgctgataaa aataaaaaag tgattaacaa aaagactgaa aaagaagata cagtgaatga
    51481 aaatgataac tttaaatata gcgatgctat agattacaaa aaagccatta aagaaggaca
    51541 aaaagaattt gatggtgata ttaaagaatg gtcacttgaa aaagatgatg gcaaacttgt
    51601 ttacaatatc gatttgaaaa aaggtaataa aaaacaagaa gttactgttg atgctaagaa
    51661 cggtaaagta ttaaagagtg agcaagatca ctaaaaagat tcgttgcatt aacgcttgta
    51721 aaatgtctta attcatatta attattaaaa taccctcaca acgcaatgta atatatccag
    51781 tatagatttg aaaggatata tgcctaacgt gttgtgaggg tttatttgca tctatttatc
    51841 catgcgaata tcgacttctt ctaaatgttt ctgatattct ttaaccttac tttctaaaaa
    51901 catttcatat ggtgcatcaa agaaatcagc taaatgcatg gcgtctttaa atttaggttc
    51961 atggtgatga ttctcccatt cccaaatttg atgtgcttca tatttagtac catatttctc
    52021 atttaattgt tgtgctaatt cgtcaatttc taaattatgt ttagttcgta agttatataa
    52081 aatatgcata ttcattgttc attaacctcg cttcttgatt taaaattcaa agatgctcta
    52141 ataatatgtg tatattctag tcctataaca taaaataatc aagctatgaa ttcggcagaa
    52201 aaaataatgt atatgatatt gccggtacag agactttaaa taacgatagc tacattgaat
    52261 aaaattgata ttcaattact acttttaaaa atatttggat aaaaataatt tgaatttgtt
    52321 ttagaattgt aaataagggg tactacttag aataacgcaa aataaataga aaaaggagac
    52381 tgaaaattat gtttggattt attggaatgt taattatcgg tggcttaatt ggatgggctg
    52441 ctggtgctat tatgggtaaa gatatcccag gtggtatttt aggcaatatt atcgcaggta
    52501 ttattggatc atgggtaggt ggcaaactat tcggacaatg gggtcctgaa ttaggaagta
    52561 tttacatctt gccagcatta attggttcaa ttatcttaat tgcaatcgta acgttaattt
    52621 taagagctat gcgtaaataa taaaaaaatt caattggaaa ttgaaataaa gatggcattc
    52681 agttgtcatc ttttttattt gcgtgaaaat ggtcaatatg tattataata tttgaaaatg
    52741 tagataagat aggattaaaa agtaataaat taatatgagg aaattttgga aataaaaaag
    52801 cgagtatttg acaaataaaa aaaggatgac gttgggttgc gccatccagg agtattatgt
    52861 tcatgaatat aaaagactgt gtagtcattt aagaactaca cccatatagt aacacatcac
    52921 ttgattaaag acaatactat ttttagagtc attaatttaa aatatgaatt aattcttttt
    52981 ttagaataga atattaagat tgatatcgaa ttagtagtca aagtgttatg gtagatatga
    53041 aatacataag gtaaggagta atattatgac gatttattta gttagacatg gcgaatcaaa
    53101 atcgaattat gataataaac attttcgatc ttatttttgt ggacaattag atgtgccgtt
    53161 aacggatact ggcacaaaaa gtgcagacga tttatgtgat tattttaaag agaaacagat
    53221 taaacatgta tatgtttcag acttattaag aacacagcaa acgtttgaac atatttttcc
    53281 atatgacatt gcatcaacga ctacgcctct attaagagaa cgttcacttg gcgtatttga
    53341 gggtgaatat aaagatgaaa tcagtgcgaa tccgaaatat gaaaaatatt tcaatgatcc
    53401 aaactttaaa gactttcgtc atagtttttc acaaaaagcg cctgaaggag aaagttatga
    53461 agatgtatat caacgcgtcg aacattttat gaatcatgtt gtcaatgaag atacacaaaa
    53521 agatgatatc gtcattgttg cacatcaagt tgtcattcgt tgtttgatgg tttattttaa
    53581 caaagtttca agggaagaag ctgtggattt aaaagttgaa aattgcaaac catatatcat
    53641 tgaatagaag tatagaggtt ctgaaaagct gtgttttata gctgccttca gaaccttttt
    53701 tgtatttgga atttaatgga agtcattctt atcaacagac ttaccaccaa tagctaatgt
    53761 gataattgcg cttacgaaca aaataattga agttataaat gacgctaatg taaaaggtgt
    53821 aaattgtgtg agcgataata tcaatgataa aaagaaccaa gagatagccc ctagaaattg
    53881 taaaatagca gaagaaattc taaaactcat ataatcatat atagctaaaa caagtgaagg
    53941 taatgcgact aataccatta taatgatgtt gagggtgaat aaatatggct gttcaaaagt
    54001 tactgtgttt ggtcctgttg gatgcattgc tgttaagaac aagcaaacaa tcgtcgataa
    54061 aattgctaaa ataataaaag taattcgaac tttcatcatg atcatccttt gtttatagag
    54121 tcaatataag tatggaatat gttaggtata tagtcaaatg cgtcaactaa tgggaatttt
    54181 aggatggata gtgaatttaa ggcaattaaa aaagcatcaa acagtaatat gctgcttgat
    54241 gcccaaatga tgactttagc taaattgatt agtcactttt aaagataaag aattgtcatg
    54301 aattaaagct catgtaatga tgtgttacat gtcgcaatga tggctttcag ttatttatcg
    54361 ataacatcac tcttgatacc tttagatttt aagaaatctt taattttatc ttgttgcttt
    54421 ttattaacat caccggcata ttttgttggc acgtcgacta cattgatttt attttgcggt
    54481 tgatagctaa gcttttcaat atcttcatca acattggcga ttgtactatt taaagctttg
    54541 aagtaattca tcattaattc aacgggtttc ttatattctt taggaatatt gttttcagtg
    54601 acaaatttct tgaaatgcaa atcgttttta acagctaagt tagataagtg gctaagtgtt
    54661 tctgcttgtt tttcagtcac ttttgtttga ctgtcaattt gtttatctag tttatgttgc
    54721 ataatatatt tgttctcaag tatatcgcta tttacagaca aatacttttc tatagcttgc
    54781 ttcatctctg catcactaat atcactattt ttcttatctg agttaaagat atcttttgtt
    54841 tctaattttt tagcgctttt aggtgcatgg atgccagtac ttgtgtgatg atcttcgtta
    54901 tcagattgat cggacgcgca acctgtaaga attaatgcag atgctaaaaa tgtacttagt
    54961 agtaatctct ttttcataat gtaatataac tccttagttt atctttaatt gaaaaaatgt
    55021 gtattcatgt ttaatagagt aacattgaat tagtttggaa tgtcacgatg accttgcaat
    55081 gaccatagac gtaaatgatt acgtgcatga gttgcttttt caatcaataa tgcgtcgttt
    55141 tgaacgttgt taaggatagc attatctata aataactgca taattggttg tatcaattta
    55201 gacgtaggta tcgtacgtaa aagcataatg atttcgttca catacttttc cttctcaata
    55261 tcatttttca tattgatttg tttgcgtaga taactggtga tctctttaat tgcttgatca
    55321 gatttcaaat agtgatgtct atcataatag attaattctt caaccgaagc tggtacataa
    55381 tatccatcat cagaaatatc aatgccagac gcctcagact cgacaacatc aaataacaca
    55441 ggatgaaaca cgaagtcatc ttcaacataa tatagctcag taccagctaa agtatctaag
    55501 ctatattgta actcttcttt actaaaagta gcgtcataaa atctttgtaa aagatatagt
    55561 agatgattag ttgataaaat accatacaag tttacagaac caagtataat acgaagaggt
    55621 aaaatatctt ttttatctat taatcctttc ttaatctcag aaatcaattg agtgattgca
    55681 tcacttctta agctatcggt tgtaccaaaa tcttcatctt caagtaacat atctaagtca
    55741 acttcgtctt ccataaatga taattctaac gcgtttttaa actctgctaa actattctca
    55801 attgtttgct cattaacaaa ttgatttaca gctttctcaa aatcaccatt gccgacttga
    55861 ttcacgaaat tttcaatatg tttacgcaca tcatttggca aaataagaca atcatattca
    55921 aagtcgtaat aataataaaa atgaatagtt aaaaagttat caaaatttat taattgcagc
    55981 ttttcaaatt tagttagttc attgtttaat cggtagttat catcactttc aagtatgcta
    56041 gaaagaatat caaactcaaa tagagtagca ttagaaaaca cttgttcaat gatgtgatta
    56101 ttattgaaaa atgctgtcat aattaattca acaagatttt gtttgttttt atttgagaat
    56161 cctttgatac catactgcct gcatattgtt tttagctcgt cattagtttt taacagtaaa
    56221 gcatgttcta atgtgacatc gtttaagcgc gtatgttgtc ttacacgtaa cacttcttca
    56281 tttaaattca tatggtttat tgtatctaac gattcgaatt caccatatgc attatctagt
    56341 acctctgaat tgatatatct taaaaagaaa ttatttacaa agaactcatc ttcgttaagt
    56401 tgataaacaa aacctagatg atttgttagc tttaaaatat taacatcgcg tttatttata
    56461 attgtgacag gattaccatt agatataagt gtttctataa atttttgttc tgtcttatcc
    56521 atatcatgat aaattttatg tatattttca gggcgtaaag catgcatcgt gacttcaaat
    56581 ccttcttttt gggtcggtaa attaaaatat tcgcatgcgt tttgtaattt tcttgggtat
    56641 ataaaatgaa catcaagcat ttttgcacac ctcgttgtat atattaagtt tatcataaca
    56701 tgattttatg tcgggataaa aaaataacag catcttaaca aatgtaagat actgtcagtg
    56761 aaatgaatga aactttagtt tctgataata tagtcaaagg catttaatgc tgcatttgca
    56821 ccagcgccca ttgaaataat aatttgtttg ttcttctgat ctgtgacatc gccagcagca
    56881 aatattccag gtacgttagt attattgtta cgatcaataa caatttcacc acgttcgttt
    56941 aattcaacag catcttttaa ccatgatgtg tttggaagta aaccaatttg aacaaagata
    57001 ccatctaagt taagtagatg ttcttcgccg gtgctcatgt cttcgtaacg tatacctgta
    57061 acatggtctt ctccgacaac ttcagtagtt ttggcatttg ttttgatatc aacatttgat
    57121 aaagaacgta aacgatcttg taacacgttg tctgctttta attcgctagc gaattcgaat
    57181 aatgtaacat gattaacgat accagcaagg tcaattgctg cttcaacccc agagttaccg
    57241 ccaccgataa ctgctacgtc tttattttca aatagaggtc cgtcacagtg agggcagaat
    57301 gcaacacctt tattaatcaa ttgctcttca cctggaatgt ttagcttacg ccaacctgca
    57361 ccagtagcaa taatgactgt tttactttct aagacagcac cgttttctaa cgtaacttta
    57421 attgcttcgt cagtcttttc gatatctgta gcacgtatac ctgtcattgc atcaatgtca
    57481 tattgatcaa tgtgcgctgc taagttagaa gaaaattcag aaccagttgt ttctttaaca
    57541 gtaatgaagt tctcaatacc agcagtatca ttaacttggc caccgatacg atcagcaact
    57601 ataccagtac gtaaaccttt acgtgctgtg taaatcgctg cactaccact agcaggacca
    57661 ccaccaacga ttaagacatc ataaggttct ttattttcaa actcagatgc atctgccgta
    57721 ctgcctagtt tcgaaagaat atcttggatt gtcatacgac cattgccaaa ttcttcgcca
    57781 tttaaaaaga cagcagggac tgccatgatg ttttcagatt cttcacggaa cactgcacca
    57841 tcaatcatag aatgcgtgat gttagggttg atcacactca ttaagttaag tgcttgaacg
    57901 acatcaggac atttttgaca cgttaaacta atgaatgttt caaaatggaa tgaaccttct
    57961 aagtttttaa tttggtcaat gattgactgt ttttctttag gtgcacgacc actaacctgt
    58021 aaaattgcta aaacaagtga gttaaactcg tgacctaatg gaatacctgc aaatgttaca
    58081 cctgtttctt cgccaggacg attgactgag aaacttggtg tacgctttaa agacttttca
    58141 gaaagtgaaa gtctaggtga catatcagaa atttctgtta ataattcttt gagttcctta
    58201 gatttttcat ctgaaccaag gctggcaacg aattcaacgt tgccctccat tagttctaat
    58261 aattgtttaa gttgttgttt taaatcagca ttaagcatgg ttgtaatgcc tccttagatt
    58321 ttacctacta aatctaaacc aggttgcaat gttttagcgc cttcttccca tttagctggg
    58381 catacttcgc cagggttttt acgaacatat tgagctgctt tgattttgtg agctaatgta
    58441 ctagcgtcac ggccaattcc gtcagcgtta atttcagatg cttgtacaac accgtctggg
    58501 tcgataatga atgtaccacg ttgagctaaa ccagtagctt catctaatac atcaaaatta
    58561 cgagtgattg tttgtgatgg gtcaccaatc atagtgtaag tgattttgct aattgcatct
    58621 gaatggtcat gccatgcttt gtgtacgaag tgagtatcag ttgatactga gaatacattt
    58681 acgcctaatt tttgtaattc ttcatattgg ttttgtaagt cttctaattc agttggacaa
    58741 acgaatgaga agtcagcagg atagaagcat actacgctcc aagaaccttt taaatcttct
    58801 tgtgtaactt ctttaaattg atcttttttt ggatcgaaag cttgcgctgt aaatggtaag
    58861 atttctttgt taattaatga cataaatatc ttcctcctaa gaatttaagt atgaattaga
    58921 actatcaatt gattgtgctt aattataata attctaatct cttagttagc attattacat
    58981 tttgatccag aatagtcaac tggataactt tgtaaagtga atgattactt ttaaaataaa
    59041 gaaagataat ataaagtgct ttgataatgg attttgtagt tgatgattta aaaggttgtg
    59101 tctatattta atatcttgat tttaatgtaa aaaatgtaaa aaaagaagat ttgtattctc
    59161 aactaagtca accttattga taatggtgtg agaatatttg ttcgagatgg atgaaggtaa
    59221 tgagtgagaa actggatttt taaagtatga gacaatattt ttaaaagttc aattattaac
    59281 ttataagcaa ataattgcta taaaaaagtt tggacgtgta caattgcaat atgaagattt
    59341 taaattaatt gtaaagtatc gaggagtggg taacgtgtca gaatatgtat ataatcttgt
    59401 gaaaaagcat cattctgtta gaaaatttaa gaataaacct ttaagtgaag acgttgttaa
    59461 gaaattggta gaagctggac aaagcgcttc gacgtcaagt ttcctgcaag catactcaat
    59521 tattggtatc gacgatgaga agattaaaga aaatttacga gaagtttctg gacaacctta
    59581 tgttgtagaa aatggctatt tattcgtctt tgttattgat tattatcgtc atcatttagt
    59641 tgatcaacat gctgaaactg atatggaaaa tgcatatggt tcaacggaag gtttgctagt
    59701 aggtgcaatc gatgcagcat tagttgccga aaatattgcg gtaactgctg aagatatggg
    59761 gtatggcatt gtctttttag gatcattaag aaatgatgtt gaacgagttc gagaaatttt
    59821 agacttacct gactatgtct tcccggtatt tggtatggca gtaggggaac ccgcagatga
    59881 cgaaaatggt gcagccaagc cacgcttacc atttgaccat gtcttccatc ataataagta
    59941 tcatgctgat aaggaaacac agtatgcaca aatggcagat tacgaccaga caatcagcga
    60001 gtactataat caacgtacaa acgggaatcg caaagaaaca tggtcgcagc aaattgagat
    60061 gttcctagga aacaaagcaa gattagatat gttagaacaa ttgcaaaaat caggcttaat
    60121 acagcgatag caagatacca aaataacccg cccccctcta gcttaaaatg ataagtatag
    60181 ctagaggggg cgggtatttc ttgcaatgaa ttagtgtgaa gttaatgcag cattatcatt
    60241 tgaatcgaaa gtatctttat cccaatgttt agttaacttg gcggtacctg taccagctag
    60301 cattgaatcg ttcacgttta atgctgttct acccatgtca atcaatggtt caacggagat
    60361 gagcacgccg gctaaagcga ctggcaagtt taacgttgac aacaccaata tggatgcaaa
    60421 tgtagccccg ccaccgacgc cagcaacgcc gaatgaacta ataatcacga cagcgattaa
    60481 cgttacaata aattgtaaat caatttctac attagcgacg ggtgcgacca taattgcaag
    60541 catggcaggg taaatgcctg cacaaccatt ttgtccaatc gacaatccaa atgtcgcagc
    60601 gaaattggca ataccttctg gcacgcctag acgtcttgtt tgtgtttgta cattcaatgg
    60661 taaggcaccc gcgcttgagc gtgatgtgaa tgcaaagatt aatacttcca aagtcttttt
    60721 aacatagcga attgggctaa tacctaacag gcttaaaata attaagtgaa tgatatacat
    60781 cgtaattaat gcagcgtacg atgcgattaa gaattttcct aaagtccaaa tggcgccaaa
    60841 gtcacttgtc gataatgtgt tggccataat tgctaataca ccgtatggcg ttaaacgtaa
    60901 gacgaacgtc acaatcgcca ttactagtga atagatagcg tcaatcgcac gcttaagcaa
    60961 ttcaccatga tcaggttgtt tgcgtgctac gcgtaaataa gcaaatccta taaacgaagc
    61021 aaatatcacg acagcaatcg tggaagttgc acgttgtcca gtgaaatcta agaatggatt
    61081 tttaggcaat aattccaaaa tttgttgtgg taacgtatgt gctgttaaat ctttcgcttg
    61141 tttagcaatt tcgcttccac gcgcttgttc agcgttacca aggttaattg ttgatgcatc
    61201 taaaccaaac accaaggcat aaacaacacc aacaattgca gcaatggtga cagtgccaat
    61261 taaaaagata aaaatgagac taccaatttt agcaaacttt tctccgattt gaattttagt
    61321 gaatgcagcg acaatagaaa tgaaaattaa aggcatcaca atcatttgca acaatgcaac
    61381 gtaaccttgt ccgacaatgt tgaaccagtc acttgttgat gtaataacat tcgaatgtgt
    61441 tccatagaca agatgtaata acacgccaaa tacaatgccg atacctaaag ctgtaaacac
    61501 acgtttcgca aaagatatat gtttgcgagc catcatgtgc aatattacga tgaaaatcac
    61561 caatacaata atattaatca gtgtaagaaa agcattcatg aacgtcactc ctcaaatttt
    61621 tgaatataat tccgactagt atgctaagaa taatattaat tttaaaatat ttcaagaggg
    61681 catttgcaat taaatgtaaa catgatgaaa ttcgaccgtt ggcataaggg aatttgtaaa
    61741 caaatcgttt tgaatttagt agagaaaggt gaaaagggtt aatagtgtta tgacctagtc
    61801 gattaaagtt gatatttgaa aatgaagcag ggaattaaac gcaataaatt aaataagttg
    61861 tcattacagc gttaataata gaaatgattt taacgggaaa aaagtgatga atatttggaa
    61921 aagatatata tcgtgtactg tcatgagaga taagatatga gaaaacaaat gtatccttga
    61981 atcgagatgt ggctatggcg gtttaataaa aattgctaac ctcatattta ttgaataaaa
    62041 agataataaa tgatcaacct tgaaaagaca cttcgaacaa acacgttgta aaaaattctg
    62101 tcgaatcaaa attagcatct tttgagggtt cgtgtgtgca ggtggtagtt caaaagctac
    62161 cgtaaaacaa atatttttgt ttgagcaaaa aggcgtttgt agaaaatgtg aatatgagtg
    62221 tgcccttgtg cgattcgtca gttactaaca cagccaaaca tttaaattga atgggcttat
    62281 aagatgaatt aatttaggaa tacacagaag ccatcccatt taaaagtaac atactacaaa
    62341 aatttattca aaattcaata ttaatcataa tgaataatta aaaaacctga aacaaattag
    62401 tttgatatgc tctcttcaaa gtagatattg aaaggagtat atcagcttgt ttcaggtttt
    62461 atgaattgag tattaaattt gaaagtaaat tacagttcta tagttttata aaagatcagt
    62521 ttttaaatca aagctatata ttattgagta ttacaggctg ggacataaat caatgttcta
    62581 tgctctacga agttatattg gcagtagttg actgaacgaa aatgcgcttg taacaagctt
    62641 ttttcaattc tagtcaacct tgccggcggg gccccaacaa agagaaattg gattcccgat
    62701 ttctacagac aatgcaagtt ggggtgggac gacgaaataa attttgcgaa aatatcattt
    62761 ctgtcccact ccctacaagt tgaataaacg tgcgaagaaa ccttttttct cttcttgagg
    62821 ttttggttct gacatttcag actgtgaagc tgtgctgtca ttttctgctt tcgcttccaa
    62881 tgaatcgttc gaagtagctt gtttagaagc ttctgcatgg gcttctgcta cttgttgttc
    62941 agcatcatgt tgcgcttctg catctattgt agcttcttta tttgaagtct cagctgcatt
    63001 gccattttta gactgctcaa cgctggcttt ttcaattgct tcagttgcag aaatttgatc
    63061 ctctttatct actttagtag tagtgccatc gcctgcattg tctgctttag tatccgtctt
    63121 agcgtctgtt gacgtattca ctccagcaac cttcgaatct ttactgtcag ctttatcttc
    63181 agtaacagca ttcttttcaa ctttatttgt tgattcagcc aatactttag cttgagatgc
    63241 ttgtgcttct tggatagcct cttgtgatgt ttggatagct tttaaggatt cgctatttga
    63301 atcgatttta gatgtaagtt gattttgaag ttcttttaat tcttgttgtt gctgatgtac
    63361 ttgattcatc atttgaccaa gtaggtgacg ttcttcgcgc attttctgga tttctaggcg
    63421 taaatcttct actaattgag cgacattttg attagtaggt aagtttttgt catcgttttt
    63481 gacaatgact tgcaggaagt ctttttcttt ttctaattca tcaaacgcta gatcataact
    63541 atttgtttgt tttactttgt cggcaatgtc tttgaaaagc tcgatatctt cttctttgaa
    63601 atcagttgct tcacgaccac gatattcagt cttgcttagt tggtaaccac gttcttctaa
    63661 atgttgaaca attttacgga cttgcttctc acttagttct acgcgttgtg caaattcttt
    63721 agttaacata ctttaaagtc ctttctgcat atgctttatg tatactgacg tttgtctacg
    63781 ggcagtgaaa cgtcagtctt gcttcataca tagtttaaac gttattgtta gtgatttcaa
    63841 gtttgtttaa aatggtttac gtaaatccat ttcttctaat aaagcataaa taatttcacg
    63901 tgcgatacca tttgcaaata catacttcaa aatataattt gatttaacac ttgtaccgta
    63961 gatatccttt gtcagtttaa tatttttctt taaaaataag tccacttttt tagtgtgacc
    64021 atcattagtt ggatgaatgt aatcaatttc agtttgtctt tcaaattgat gttcgtataa
    64081 aaactgtcta cggtgttcaa taatgagtcc attatcttgt gttaaatctg caggttcttt
    64141 agggacttcc atcattaaaa agttaatact tctagaatga acaaggtttg ataataagtt
    64201 tagttcatgt tcaatttgat gtaatgtttg cgtcattaac tcatcatggt tgggtaggtc
    64261 ttttgcgaat aaataaatta aaaatgctga attggttgtg gcttcataat gtgcagttgc
    64321 tagactgaca acaacatcgt tttctaagcc tacgataaag acataatcgt tttcggtttt
    64381 gttgttttct aatgagcgtt tgatgatacg tttatcttca gttaaagata aatttaactt
    64441 agtatcgtaa agtttaagtg cttcgttgta atgtggatct ttgacagatt gaatggtttt
    64501 aaattccata agaacacctc cccaatttaa ataatattat agcataatcg cctgctgtaa
    64561 aagactgttc ataaactttt aaatggtata aaaaactgta ctatcttaaa ttagacagta
    64621 cagtaatctc attttgaatt cagtgtgata actaagcttt gggaccttta gatgcttcag
    64681 caaaatgtgt aatatcaatc tcttcataag ctgaattatt ttcatgcact tcttgatgtg
    64741 atgatttgtc acgaaccgct acaactaaca ttttatcgtc taaaataagt tgtttatatt
    64801 tttctaattc atcaggcgct aagttgtaac gtgataaaac tgcatgttca ccatcttctc
    64861 ctgttaacag tttagtcatt ctatcactaa atgttccact tgttgagata agggagattt
    64921 cagagtcgtg taagtcattt aggtgtaatt tacttttact aataattgtt agctctgatt
    64981 ctaaataacc ttcagatttc ttttgattga ttacgttgta taattcgcca gtgtcattta
    65041 ctacagtaat atctgccata gttgtcgccc ctttaaaaat ttgtttattt aatcttttac
    65101 ccttcttatt ataaagtaaa acccttacat tattaagtta taagtcttca ttcgcattaa
    65161 acgtctctgt acatttataa aactattaaa agattaccta agcagttatt gaaaaaatgc
    65221 cgaaaatttg ctattatcgt taaataattt acataaactc atataatcta aagaatatgg
    65281 ctttagaagt ttctaccatg ttgccttgaa cgacatgact atgagtaaca acacaatact
    65341 aggagtagct tcagccatta aattgtaacc atggtgggtg atttatatca ttttatatga
    65401 tggtcacagt ttatttgatg aaacttcttt tacattgatt gcatgacaaa ttcgttatgc
    65461 atgttcgttc actcataaac cctgaaacta ttatttagtt tggggatttt tttgtatcta
    65521 gcaccaattt aagagcaaaa tgtttcacac aaatctgagg aggttttaag agtggagtta
    65581 ctaggacaaa aagtaaagga agacggcgtt gtcattgatg agaagatttt aaaagtcgat
    65641 ggatttttaa atcatcaaat tgatgcaaag ttaatgaatg aagttggtcg cactttttac
    65701 gagcaattta aagataaagg gattactaaa atcttaacca ttgaagcttc cggtatcgca
    65761 cctgcaatca tggctgcact gcattttgat gtgccatgtt tatttgcgaa aaaagcaaaa
    65821 cctagcactt tgacggatgg ttattatgaa acatctattc attcatttac taaaaataaa
    65881 acaagtacgg tcattgtttc aaaagaattt ttatcagaag aagatactgt actcatcatc
    65941 gatgactttt tagcaaatgg tgatgcttca ttaggattat acgatatcgc acagcaagcg
    66001 aatgctaaga cagctggtat tggtattgtt gttgaaaaga gtttccaaaa tgggcatcaa
    66061 cgtttagaag aagcaggttt aacagtttct tctctctgca aggttgcttc actagaagga
    66121 aacaaagtga cattggtggg agaagaataa tgaaaaattt aatcctaagt gttcaacatc
    66181 ttttagctat gtacgcaggt gccatcttag ttccaatcat tgttggtaca agtttgaagt
    66241 ttacacctga acaaatcgct tacttagtta cagtagatat atttatgtgt ggggttgcca
    66301 catttttaca agccaataaa gtaacaggaa caggattacc aatcgttctt ggatgtacat
    66361 tcacggctgt tgcgcccatg attttaattg gtcaaacgaa aggaatagat gtactttatg
    66421 gttcgctatt tttatcaggg atattagtta ttatcatcgc gcctttcttt tcacatcttg
    66481 taaaattctt cccaccagta gtaacgggta gtgttgttac tatcattggt atcaatttaa
    66541 tgccagtagc aatgaattac ttagctggag gtcaaggtgc aaaggactat ggagatgtta
    66601 agaacatttt gttaggttta atgacattaa tcattattct tgttttacaa agattcacaa
    66661 ctggatttat taagagtatt gccatattaa ttggactcgt tttaggaacg ataggtgctg
    66721 gcttacttgg gatggtcgat attaatcaag tcaatcatgc cggttggtta ggcatcccag
    66781 tgccgtttag attctctgga tttagctttg atgtgacatc gacgttagtg ttctttattg
    66841 tagctatcgt tagtttaatt gagtcgacag gtgtctatca tgcgttaagt gaaattaccg
    66901 gtaagaagtt agaaagaaaa gattttcgta aaggttatac tgcggaaggt ctagcgatag
    66961 tgttaggttc tatattcaat tcatttccgt atacagccta ttcgcaaaat gtaggacttg
    67021 tttctttatc cggcgctaag aaaaataatg ttatatacgg catggtcgtg ttattactta
    67081 tatgtggttg tatacctaag cttggtgcat tagcaaatat cataccgcta cctgtgttag
    67141 gtggtgcgat gatagctatg tttggcatgg taatggcata tggtgttagt atattaggtc
    67201 atatcgattt taaaaatcaa aacaatttat taattatcgc tgtatcagta ggattaggta
    67261 ctggtataag cgctgtacca caagcattta aaggtttagg tgaacaattt gcatggttga
    67321 ctcaaaacgg aattgtttta ggcgcaatct ctgcaattat tcttaatttc ttttttaatg
    67381 gaataaagta taaacaaacg gaagaaaatg tgaaataata taactaatta atttgaaaaa
    67441 tggaggctgt ttttaatgtg ggaaagtaaa tttgcaaaag aatcattaac gtttgatgat
    67501 gtgttattaa ttccagcaca atctgatatt ttaccgaaag acgttgattt aagcgtacaa
    67561 ttatcagaca aagttaaatt aaatattcca gttatttctg ctggtatgga tactgtaact
    67621 gaatctaaaa tggcgattgc tatggctcgt caaggtggtt taggtgttat tcataaaaat
    67681 atgggcgttg aagaacaagc ggacgaagtt caaaaagtaa aacgctcaga aaatggtgtc
    67741 atttcaaacc catttttctt aacgccagaa gaaagcgttt atgaagcaga agcattaatg
    67801 ggtaaatacc gtatttcagg tgtaccaatt gttgataata aagaagatcg caacttagta
    67861 ggtattttaa caaaccgtga cttacgtttt attgaagact tctcgattaa aattgtagat
    67921 gtaatgacgc aagaaaattt aattacagct ccagtgaata caacacttga agaagcagaa
    67981 aaaattctcc aaaaacataa gattgaaaag ttaccattag ttaaagacgg acgtctagaa
    68041 ggtcttatta ctattaaaga tattgaaaaa gtaatcgaat tccctaatgc agcaaaagat
    68101 gaacatggtc gtctacttgt agccgcagca atcggtattt caaaagacac tgatattcgt
    68161 gctcaaaaat tagtcgaagc aggtgtggat gtcttagtta tcgatacagc acatggtcac
    68221 tctaaaggcg ttatcgatca agtgaaacat attaagaaga cttacccaga aatcacatta
    68281 gtagctggta acgtagcaac tgcagaagca acaaaagatt tatttgaagc gggtgcagat
    68341 attgttaaag ttggtattgg cccaggttca atttgtacga cgcgtgttgt agcaggtgtt
    68401 ggtgtaccac aaattacagc aatttatgat tgtgcaactg aagcacgcaa acatggtaaa
    68461 gctatcattg ctgatggtgg tattaaattc tcaggagata tcattaaagc attagctgct
    68521 ggtggacatg cggttatgtt aggtagctta ttagcaggta ctgaagaaag cccaggcgca
    68581 acagaaattt tccaaggtag acaatataaa gtataccgcg gtatgggctc tttaggtgcg
    68641 atggaaaaag gttcaaacga ccgttacttc caagaagaca aagcgcctaa gaaatttgtt
    68701 cctgaaggta tcgaaggacg tacggcatat aaaggtgcgt tacaagatac aatttaccaa
    68761 ttaatgggcg gtgtgcgtgc tggtatgggt tatactggtt cacacgattt aagagaatta
    68821 cgcgaagaag cacaattcac acgtatgggc cctgctggtt tagcagaaag tcacccacat
    68881 aatattcaaa ttacgaaaga atcaccgaac tactcattct aattaagata aaggagaacg
    68941 acaaatatgg aaatggcaaa agaacaagag ttaatccttg tcttagactt tggtagccaa
    69001 tacaaccaat taattacacg ccgaattcgt gaaatgggcg tttatagtga attacacgat
    69061 catgaaattt caattgaaga aattaagaaa atgaatccaa aaggtattat cttatcaggt
    69121 ggtccaaatt cagtttatga agaaggttca tttacaattg atccggaaat atataattta
    69181 ggaattccag tacttggtat ttgttacggc atgcaattaa ctactaaatt actaggtggt
    69241 aaagttgagc gtgccaatga acgtgaatac ggtaaagcaa tcattaatgc gaagtcagat
    69301 gagttattcg ctggcttacc agcagaacaa actgtttgga tgagtcattc tgataaagtt
    69361 attgaaattc cagaaggctt tgaagttatc gctgatagcc caagtacaga ctatgcagca
    69421 atcgaagata agaaacgtcg catttatggt gttcaattcc atccagaagt acgtcataca
    69481 gaatatggta atgatttgtt aaacaacttc gtacgtcgtg tttgtgagtg taaaggtcaa
    69541 tggacaatgg aaaactttat cgaaattgaa attgaaaaga ttcgtcaacg cgtaggcgac
    69601 cgtcgtgtat tatgtgcgat gagtggtggc gtagattcat ctgttgttgc tgtattattg
    69661 cataaagcaa ttggggatca actaacatgt atctttgtag accatggctt acttcgtaaa
    69721 ggtgaaggcg acatggttat ggaacaattc ggtgaagggt tcaacatgaa tattattcgt
    69781 gttaatgcga aagatcgttt tatgaataaa ttaaaaggtg tttcagatcc tgaacaaaaa
    69841 cgtaaaatca ttggtaatga atttgtatat gtatttgatg atgaagcatc aaaactgaaa
    69901 ggtgtagact tccttgcgca aggaacactt tatacagacg ttatcgaatc aggtaccaag
    69961 acagcacaaa caatcaaatc acatcacaat gttggtggat taccagaaga catggaattc
    70021 gaattaatcg aaccaatcaa tacattgttt aaagatgaag tacgtaaatt aggtattgaa
    70081 ttaggtattc cagaacattt agtgtggaga caaccattcc caggacctgg tcttggtatt
    70141 cgtgtacttg gagaaattac tgaagataaa ctagaaatcg ttagagaatc agacgcgatt
    70201 ttacgccaag tgattagaga agaaggtctt gaaagagaaa tttggcaata cttcacagtg
    70261 ttaccaaaca ttcaatcagt aggtgttatg ggagactacc gtacgtatga tcacacagta
    70321 ggtattcgtg cagtaacatc tatcgacggt atgacaagtg acttcgcacg catcgattgg
    70381 gaagtcttac aaaagatttc tagtcgtatc gtaaacgaag tagatcacgt caaccgcgta
    70441 gtctatgaca ttacatcaaa accaccaagc acaattgagt gggaataatt atatatagca
    70501 aatgataggc tggagttacc gttactatgc ggtttctagc ctttttggtg tgcttggcat
    70561 gggcaacatc atgacgtgaa ataatgggaa tcatctcttt cttatatttt gagacggaga
    70621 tgatgagaag ttagacaaaa atgtagaaga agtatttaac gacaatccta gtcatacaat
    70681 gattgtagat gatatccgtt agaggagtga gagaaaattt atttgttact aagagacaag
    70741 agttttaaat aaatatcttt tgtactacat aaaagaaagt ggtcgttatc atagaatatt
    70801 tggcgataag gtaaatctga ttatgttaga acctatattt attgatgatt tagtgaggtt
    70861 tgagaagaca agaatgattg aattacaatt gacaattgac accccttaga ttaatattaa
    70921 tttattagaa gtcaactaac gcctcttagc aatgcgaaac acagttggcc ttttttttgt
    70981 aataggagaa tttatagtta tggataaatc acttgaaatt aacttaaaag aattagaagg
    71041 cataatgtat aaagaaaaag taatcgacgt taaagagtat gataatttaa aagtaacttt
    71101 tgagacacta aacgatgctc ctaaa
//
LOCUS       DBNENW010000002        53473 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNENW010000002 DBNENW010000000
VERSION     DBNENW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 53473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.002"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            33..476
                     /locus_tag="AC1LW8_000073"
     CDS             33..476
                     /locus_tag="AC1LW8_000073"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIP4771943.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIVMQTSVDKHNWMFKSYLS"
     gene            complement(597..1307)
                     /gene="deoD"
                     /locus_tag="AC1LW8_000074"
     CDS             complement(597..1307)
                     /gene="deoD"
                     /locus_tag="AC1LW8_000074"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP4771944.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            1622..2284
                     /gene="deoC"
                     /locus_tag="AC1LW8_000075"
     CDS             1622..2284
                     /gene="deoC"
                     /locus_tag="AC1LW8_000075"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIP4771945.1"
                     /translation="MHSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQEIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            2565..3866
                     /locus_tag="AC1LW8_000076"
     CDS             2565..3866
                     /locus_tag="AC1LW8_000076"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIP4771946.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIIIE"
     gene            3880..4230
                     /locus_tag="AC1LW8_000077"
     CDS             3880..4230
                     /locus_tag="AC1LW8_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIP4771947.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCFPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(4382..4852)
                     /locus_tag="AC1LW8_000078"
     CDS             complement(4382..4852)
                     /locus_tag="AC1LW8_000078"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIP4771948.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            5163..6347
                     /locus_tag="AC1LW8_000079"
     CDS             5163..6347
                     /locus_tag="AC1LW8_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIP4771949.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            6347..7540
                     /gene="ldmS"
                     /locus_tag="AC1LW8_000080"
     CDS             6347..7540
                     /gene="ldmS"
                     /locus_tag="AC1LW8_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0034026 - L-amino-acid alpha-ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate--L-methionine ligase LdmS"
                     /protein_id="HIP4771950.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            7939..8610
                     /locus_tag="AC1LW8_000081"
     CDS             7939..8610
                     /locus_tag="AC1LW8_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HIP4771951.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(8740..9543)
                     /gene="coaW"
                     /locus_tag="AC1LW8_000082"
     CDS             complement(8740..9543)
                     /gene="coaW"
                     /locus_tag="AC1LW8_000082"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HIP4771952.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            9967..10827
                     /locus_tag="AC1LW8_000083"
     CDS             9967..10827
                     /locus_tag="AC1LW8_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4771953.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            10939..11469
                     /gene="rpoE"
                     /locus_tag="AC1LW8_000084"
     CDS             10939..11469
                     /gene="rpoE"
                     /locus_tag="AC1LW8_000084"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIP4771954.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            11805..13415
                     /locus_tag="AC1LW8_000085"
     CDS             11805..13415
                     /locus_tag="AC1LW8_000085"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIP4771955.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(13524..14045)
                     /locus_tag="AC1LW8_000086"
     CDS             complement(13524..14045)
                     /locus_tag="AC1LW8_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HIP4771956.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            14263..15123
                     /gene="fdaB"
                     /locus_tag="AC1LW8_000087"
     CDS             14263..15123
                     /gene="fdaB"
                     /locus_tag="AC1LW8_000087"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb fructose-bisphosphate aldolase FdaB"
                     /protein_id="HIP4771957.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            15589..16848
                     /locus_tag="AC1LW8_000088"
     CDS             15589..16848
                     /locus_tag="AC1LW8_000088"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP4771958.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGMELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVVLNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            16937..17272
                     /locus_tag="AC1LW8_000089"
     CDS             16937..17272
                     /locus_tag="AC1LW8_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIP4771959.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            17510..18937
                     /locus_tag="AC1LW8_000090"
     CDS             17510..18937
                     /locus_tag="AC1LW8_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIP4771960.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPKKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLELKSIAGYFK"
     gene            19184..20500
                     /gene="rho"
                     /locus_tag="AC1LW8_000091"
     CDS             19184..20500
                     /gene="rho"
                     /locus_tag="AC1LW8_000091"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIP4771961.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            20618..20872
                     /locus_tag="AC1LW8_000092"
     CDS             20618..20872
                     /locus_tag="AC1LW8_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIP4771962.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            21219..21818
                     /locus_tag="AC1LW8_000093"
     CDS             21219..21818
                     /locus_tag="AC1LW8_000093"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIP4771963.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            21819..22895
                     /gene="prfA"
                     /locus_tag="AC1LW8_000094"
     CDS             21819..22895
                     /gene="prfA"
                     /locus_tag="AC1LW8_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIP4771964.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            22882..23718
                     /gene="prmC"
                     /locus_tag="AC1LW8_000095"
     CDS             22882..23718
                     /gene="prmC"
                     /locus_tag="AC1LW8_000095"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIP4771965.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEDIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            23802..24848
                     /locus_tag="AC1LW8_000096"
     CDS             23802..24848
                     /locus_tag="AC1LW8_000096"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP4771966.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAASNHIIKGCEL"
     gene            24845..25264
                     /locus_tag="AC1LW8_000097"
     CDS             24845..25264
                     /locus_tag="AC1LW8_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HIP4771967.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            25371..25895
                     /locus_tag="AC1LW8_000098"
     CDS             25371..25895
                     /locus_tag="AC1LW8_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIP4771968.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            25922..27160
                     /gene="glyA"
                     /locus_tag="AC1LW8_000099"
     CDS             25922..27160
                     /gene="glyA"
                     /locus_tag="AC1LW8_000099"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIP4771969.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            27188..27817
                     /gene="upp"
                     /locus_tag="AC1LW8_000100"
     CDS             27188..27817
                     /gene="upp"
                     /locus_tag="AC1LW8_000100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIP4771970.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            27841..28968
                     /gene="wecB"
                     /locus_tag="AC1LW8_000101"
     CDS             27841..28968
                     /gene="wecB"
                     /locus_tag="AC1LW8_000101"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIP4771971.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            29036..29488
                     /locus_tag="AC1LW8_000102"
     CDS             29036..29488
                     /locus_tag="AC1LW8_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIP4771972.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            29509..30237
                     /gene="atpB"
                     /locus_tag="AC1LW8_000103"
     CDS             29509..30237
                     /gene="atpB"
                     /locus_tag="AC1LW8_000103"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIP4771973.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            30280..30492
                     /gene="atpE"
                     /locus_tag="AC1LW8_000104"
     CDS             30280..30492
                     /gene="atpE"
                     /locus_tag="AC1LW8_000104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIP4771974.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            30689..31210
                     /locus_tag="AC1LW8_000105"
     CDS             30689..31210
                     /locus_tag="AC1LW8_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIP4771975.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            31210..31749
                     /locus_tag="AC1LW8_000106"
     CDS             31210..31749
                     /locus_tag="AC1LW8_000106"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIP4771976.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            31771..33279
                     /gene="atpA"
                     /locus_tag="AC1LW8_000107"
     CDS             31771..33279
                     /gene="atpA"
                     /locus_tag="AC1LW8_000107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIP4771977.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            33310..34176
                     /gene="atpG"
                     /locus_tag="AC1LW8_000108"
     CDS             33310..34176
                     /gene="atpG"
                     /locus_tag="AC1LW8_000108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIP4771978.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            34198..35610
                     /gene="atpD"
                     /locus_tag="AC1LW8_000109"
     CDS             34198..35610
                     /gene="atpD"
                     /locus_tag="AC1LW8_000109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIP4771979.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            35630..36034
                     /locus_tag="AC1LW8_000110"
     CDS             35630..36034
                     /locus_tag="AC1LW8_000110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIP4771980.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            36372..36464
                     /locus_tag="AC1LW8_000111"
     CDS             36372..36464
                     /locus_tag="AC1LW8_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4771981.1"
                     /translation="MLKSKFHSPLSTVDISFFESSYIFSYQDTL"
     gene            36596..36829
                     /locus_tag="AC1LW8_000112"
     CDS             36596..36829
                     /locus_tag="AC1LW8_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HIP4771982.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            36940..38205
                     /gene="murA"
                     /locus_tag="AC1LW8_000113"
     CDS             36940..38205
                     /gene="murA"
                     /locus_tag="AC1LW8_000113"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP4771983.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            38239..38679
                     /gene="fabZ"
                     /locus_tag="AC1LW8_000114"
     CDS             38239..38679
                     /gene="fabZ"
                     /locus_tag="AC1LW8_000114"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIP4771984.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(38737..39177)
                     /locus_tag="AC1LW8_000115"
     CDS             complement(38737..39177)
                     /locus_tag="AC1LW8_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HIP4771985.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            39371..39766
                     /locus_tag="AC1LW8_000116"
     CDS             39371..39766
                     /locus_tag="AC1LW8_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP4771986.1"
                     /translation="MLNKIVIVERLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            40155..40850
                     /gene="sceD"
                     /locus_tag="AC1LW8_000117"
     CDS             40155..40850
                     /gene="sceD"
                     /locus_tag="AC1LW8_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HIP4771987.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      41192..41294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41374..42063
                     /gene="tenA"
                     /locus_tag="AC1LW8_000118"
     CDS             41374..42063
                     /gene="tenA"
                     /locus_tag="AC1LW8_000118"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HIP4771988.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            42056..42886
                     /gene="thiD"
                     /locus_tag="AC1LW8_000119"
     CDS             42056..42886
                     /gene="thiD"
                     /locus_tag="AC1LW8_000119"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIP4771989.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRRLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            42870..43670
                     /gene="thiM"
                     /locus_tag="AC1LW8_000120"
     CDS             42870..43670
                     /gene="thiM"
                     /locus_tag="AC1LW8_000120"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIP4771990.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQGLLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVEKYV"
     gene            43663..44304
                     /gene="thiE"
                     /locus_tag="AC1LW8_000121"
     CDS             43663..44304
                     /gene="thiE"
                     /locus_tag="AC1LW8_000121"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIP4771991.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIENTVNRFKDFFNN"
     gene            44390..45262
                     /gene="yidC"
                     /locus_tag="AC1LW8_000122"
     CDS             44390..45262
                     /gene="yidC"
                     /locus_tag="AC1LW8_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIP4771992.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(45876..46523)
                     /locus_tag="AC1LW8_000123"
     CDS             complement(45876..46523)
                     /locus_tag="AC1LW8_000123"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP4771993.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKLYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(46551..48035)
                     /gene="cls"
                     /locus_tag="AC1LW8_000124"
     CDS             complement(46551..48035)
                     /gene="cls"
                     /locus_tag="AC1LW8_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIP4771994.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            48201..48494
                     /gene="csoR"
                     /locus_tag="AC1LW8_000125"
     CDS             48201..48494
                     /gene="csoR"
                     /locus_tag="AC1LW8_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HIP4771995.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            48506..48715
                     /gene="csoZ"
                     /locus_tag="AC1LW8_000126"
     CDS             48506..48715
                     /gene="csoZ"
                     /locus_tag="AC1LW8_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative copper chaperone CsoZ"
                     /protein_id="HIP4771996.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVI"
     gene            48843..48980
                     /locus_tag="AC1LW8_000127"
     CDS             48843..48980
                     /locus_tag="AC1LW8_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lmo0850 family protein"
                     /protein_id="HIP4771997.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(49243..50445)
                     /locus_tag="AC1LW8_000128"
     CDS             complement(49243..50445)
                     /locus_tag="AC1LW8_000128"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIP4771998.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            50763..51833
                     /locus_tag="AC1LW8_000129"
     CDS             50763..51833
                     /locus_tag="AC1LW8_000129"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIP4771999.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIISTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            51848..53206
                     /locus_tag="AC1LW8_000130"
     CDS             51848..53206
                     /locus_tag="AC1LW8_000130"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIP4772000.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQVINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT        19010 a         7834 c        10258 g        16371 t
ORIGIN      
        1 agacgtagac aattttaagg agtgtattaa atatgagtaa tcaacaagat gttgtaaaag
       61 aattgaatca acaagtagca aactggacag tagcttacac aaagctacat aatttccact
      121 ggtatgtgaa aggacctaac ttcttctcat tacacgttaa atttgaagaa ttatataatg
      181 aagcaagcca atatgtagac gaattagctg aaagaatttt agcggtagga ggaaaccctg
      241 taggtacatt aactgaatgc ttagagcaat caattgttaa agaagctgcg aaaggttact
      301 ctgcagaaca aatggttgaa gaattatcac aagacttcac aaatatctca aaacaattag
      361 aaaacgcaat cgaaattgct ggtaatgctg gcgatgatgt atcagaagat atgtttatag
      421 ttatgcaaac atcagtagat aaacataatt ggatgtttaa atcttactta agctaatcta
      481 cagataagta ataatataaa taaaagccaa cacataaatg gggatacgcc cagaatgtgt
      541 tggctttttg agtttaatcg cattgttaaa gagcaattaa agtcaattta aataagttac
      601 tctgcgattt ctaaagcgat ttccatcatt tgtgtaaatg aattttgacg ttcttcaggt
      661 gtagtagctt cgtcacgtaa aatatgatca cttactgtga aaataccaag tgctttttta
      721 ccagcatgaa tcgcatttaa atataaacca gctgattcca tttcgatacc taaaataccc
      781 atttttttcc aagcatcatt gaatgttgga tcggcattgt aaaatgtatc agaagataat
      841 acgttaccta cgtgtgtagt agcaccgatt tggtcagcga catttttagc tttagttact
      901 aactcgaagt cagcgatagg cgcgaaatga cctggaatat tgtattgatc tacataattt
      961 gaattagttg atgcagcttg tgcaataata acatcgtata agttaacatt ttcttgtaat
     1021 gcgccacaag aaccgatacg aatgattgta tctacatcaa agaagttgta taactcatat
     1081 gagtaaatac caatacttgg aatacccata ccagaaccca ttacagaaac ttctttacct
     1141 ttatatgtac cagtgtaacc aaacatgtta cgtacatcgt taaattgttc aacattttct
     1201 aaaaaattat cagcaatata ttttgcgcgt agcggatcgc caggcattaa tactgtttta
     1261 gcaattttta ctccatttgg ttgaatatgt ggtgtacctt ttgtcatttg ttatcttctc
     1321 cttaatattc attaataatt ttaagataac atttgtttcc cttttttgcg aatttttgta
     1381 ctatagaatt aaagaattat aaaaatataa tcctgattag cttgttgtca agtcatcgtt
     1441 cataatgaag atatcacgtt caattgtatt gtttgtttat gggaaatgaa ttaatgtaat
     1501 agtatatgta tgcggttaca taaaaagcga acatctaacc tgatatttaa atgaacctga
     1561 cgctcaatca actaatttac aaccgtattt ttataatcaa ccataaagga ggagatagaa
     1621 aatgcatagt gcaaaattga ttgatcacac tttattgaag cctgagtcaa cacgtacgca
     1681 aatcgatcaa atcatcgatg aagcgaaagc ataccatttt aaatctgtat gtgtgaatcc
     1741 aacgcatgtt aaatatgcag cagaacgact agctgattca gaggtgttag tttgtacggt
     1801 aataggattc ccattaggtg catcgacaac tgcgacgaaa gcatttgaaa cagaagatgc
     1861 gattcaaaat ggtgcagatg aaattgacat ggtcatcaac atcggcgcat taaaagatgg
     1921 acgttttgat gatgtacaac aagaaattga agcagtggtt aaagctgcga aaggtcacac
     1981 agtaaaagtg attattgaga cggtattgtt ggaccatgac gaaatcgtaa aagcgagtga
     2041 attaacaaaa gcggctggtg cggacttcgt taaaacttca acaggttttg caggtggcgg
     2101 tgcgactgca gaagacgtta aattaatgaa agatacagta ggtgctgatg tagaagtaaa
     2161 agcatcaggt ggcgtacgta atttagaaga tttcaataaa atggttgaag caggtgcgac
     2221 acgtattggt gcgagcgcag gcgttcaaat tatgcaaggt ttagaagcag attcagatta
     2281 ctaattttat aaaaaaatat gtaagcatgg gacatcgtaa ccatatataa gtatgtcgat
     2341 atcagcttta agacatcata aatgccatga tgattctaag ttaatttaat agggacttta
     2401 acgttataac caatctattt aagaataata acatcgcatt gaaattgtga tattaaaaat
     2461 gaaatcgata gcttgctata ataattttcg cttgtgcttt taaaaatgga ctagagaaag
     2521 cgcagatgct ttgtcctaag ctttttaaga aaaggatgat aaaaatgaga atgatagaca
     2581 ttattgagaa aaagcgtgac ggtcatacac taacaacgga agaaattaat ttctttattg
     2641 acggctatgt taaaggggat attcctgatt accaagcatc aagtttagca atggcgattt
     2701 atttccaaga tatgaatgat gatgagcgtg cagcattaac gatggctatg gttaattctg
     2761 gtgatatgat agatttgagt gatattaaag gtgtcaaagt agataagcac tcaacaggtg
     2821 gtgtaggaga tacaactaca ttggttttag caccattagt agcagctgta gatgttcctg
     2881 ttgcaaaaat gagtgggcgt ggattaggtc atacaggtgg tacgattgat aaattagaag
     2941 caattgatgg ttttcatgtt gaaatagatg aagcaacatt tgtgaaattg gttaatgaaa
     3001 ataaagtggc agttgtagga caatcaggaa atttaactcc tgcagacaaa aaattatatg
     3061 ccttaaggga tgttactggt actgtcaatt caataccatt aattgcctct tcaattatga
     3121 gtaaaaagat tgctgctggt gcagatgcaa ttgtattaga tgtaaaaact ggtagcggtg
     3181 catttatgaa aacattagaa gatgctgaag cattagcgca cgcaatggta cgtattggta
     3241 ataatgtggg acgtaatacg atggcgatta tttctgatat gaatcagcca cttggacgtg
     3301 cgattggcaa tgcgcttgag ttacaagaag caattgatac gttgaaagga caaggtccga
     3361 aagatttaac agaacttgta ttaacattag gttctcaaat ggttgtactt gcaaacaaag
     3421 ctgaaacgct tgaagaagcg agagcgttgc taattgaggc aattaattca ggtgcagcat
     3481 tagaaaaatt caaaacattt attaaaaacc aaggtggcga cgaaactgtt attgaccatc
     3541 cagagcgttt gccacaagct caatatcaaa ttgaatataa agctaaaaaa tcaggttatg
     3601 tgactgaatt agtctctaac gatataggtg tcgcttcgat gatgttagga gcgggacgtt
     3661 taacaaaaga ggatgatatt gatttagcgg ttggtattgt tttaaataaa aaaattggtg
     3721 ataaagtaga agaaggagaa tcattattaa cgattcacag taatcgccaa gatgtagacg
     3781 atgttgttaa aaaattagat tcaagcataa caattgctga tcatgttgta tcgcctacat
     3841 taattcataa aatcattata gaataggagt tgaagctgat tgaacatact aagatattta
     3901 ttaggaatag gttttagtgt aataggtgta ttgcatttta cacgagaacg acaatttaga
     3961 aatatcatac cgaaatgttt cccacttcga aaaacagcgg ttcttgttac aggaatcttt
     4021 gagatattat ttggacttgc acttttaatt aaaagaccat ctcaatgctt gaaaaataca
     4081 atcaatctat tcttgctagc tgtattccca gctaatatct atatggcagt taaaaagtta
     4141 cctttaggag ataaacaatt gccaacatgg caactatatt taaggttacc tatgcagttc
     4201 gtgttaatgg gactaattaa gaaattatag tggttctcaa ataaatcgga caaatgttaa
     4261 agattttact ttttcatttg ttcgattttt tgactttgaa gcataataaa aagcagttgg
     4321 acaatggcga ttgtaacatc attgttcgac tgcttttctg attaacttga ttgactaaga
     4381 tttattttcc tgtaccgaaa acatcatgcc attcgtttcg tttgtctaga aatgcttgag
     4441 cgatagtttt agcaccttct aatgaatgac ttgctgccca gccacattgt acttcattac
     4501 aagcaggcac ttcagtagca tttagcacat catttaaagt tgcttcaaca atatttaata
     4561 catcatcata attatcatga ttaataaatg atacatagaa accagtttgg caacccatag
     4621 gacttaaatc aacaactttg tcactatgat ttctaatatt ttcagccatt aaatgttcta
     4681 atgaatgtag tccgggcata tccatatgtt ctttgtttgg ttgtttgaaa cgaatgtcgt
     4741 atttgtgaat gacatctccg tttaatcctt ccatcgttcc cgctaatcta ataaatgggg
     4801 caaccacttt agtatgatct aaattgaaac tttcaacatt catttttgtc attttgaatt
     4861 tcctcctatt aactactcaa aactataaaa cgcttatgat ttgttgcatt ataaaactta
     4921 agtttgttgt aagttgtgtt tctaacaata cttaaattgt aacaataggg cttatgtttt
     4981 acaatttgaa actgttttat aaatttatga aaggctttta gttattagaa aattttgtaa
     5041 ataaagaaaa actagatgac atcatgagat aaaaccgata gaataagtaa taattcattt
     5101 tgaaagaaca aaatattgtg gatgattaaa atgagtgtag ggtaaaaggg agatagtaaa
     5161 aaatgagtga aaaacaacaa attctcgatt atatagaaac aaataaatat agttatatcg
     5221 aaatcagtca tagaattcat gaacgtcctg aacttggtaa tgaagaaata tttgcgtctc
     5281 gaactttaat agatcgtttg aaagagcatg attttgaaat tgaaaccgag attgctgggc
     5341 atgcaactgg gtttatagcg acatatgatt caggtcttga cgggccagct ataggttttt
     5401 tagcagaata cgatgctttg ccagggttgg gtcatgcttg tggtcataat atcattggaa
     5461 ctgcaagtgt gcttggtgca attggtttga agcaagtgat tgaccaaatt ggtggtaaag
     5521 tagtcgttct tggatgtcca gctgaagaag gtggggaaaa tggtagcgct aaagcttctt
     5581 atgtcaaggc tggtgtgatt gatcaaatag acattgcctt aatgattcat ccgggaaatg
     5641 aaacttataa aacgattgat actttggcag tggatgtttt agatgttaaa ttttacggaa
     5701 aaagtgctca tgcctctgaa aatgcagatg aagcgttaaa tgcattagac gctatgatta
     5761 gttattttaa tggtgtagca caactacgac aacatattaa aaaagatcaa cgtgtgcatg
     5821 gtgtgatttt agatggcggg aaagcagcta atattattcc agactataca catgctcgtt
     5881 tttatactag agcaatgacg cgtaaagaat tggatatatt aacagaaaaa gtaaatcaaa
     5941 tcgcacgtgg agctgcgata cagactggtt gtgattatga atttggtcga attcaaaacg
     6001 gtgtgaatga attcattaaa acgccgaaat tagatgattt atttgctaaa tatgctgaag
     6061 aagttggtga agcagttatt gatgatgatt ttggttatgg ctctacggat acagggaacg
     6121 taagtcatgt tgtgccaaca atacatcctc atattaaaat aggatcacgt aatttagtag
     6181 gacatacgca tagatttaga gaagcggctg cgagtgtaca tggtgatgaa gcattaatta
     6241 aaggcgctaa aataatggcg ttgatggggt tagagttaat tacaaatcaa gacgtttatc
     6301 aagacattat tgaagagcat gcgcatttga aagggaatgg gaagtaatga caaataatgg
     6361 taatgagccg aacttaacgt taagtgattt atatgacaaa gatgttgtct atacgtcaag
     6421 accttcgtat atatcgaatc catggttgaa accggacgaa catcaatcta attttttaac
     6481 tggtagggag ttattaatag caaatcaatt acctgttatt gttcacgaag ctagtgcgac
     6541 agacaaattg catcagctat ttcaagttat tggtaaggaa gtaccgaact caatttatac
     6601 atttaacaat cagcaatctt acgaaaactt gatcaaacaa ttggcacata aggaaaataa
     6661 aaagatttat tttcaatata ttcatgatga aacaatcttg aatcagcaat attatgcatt
     6721 agataaaacg ttatttgttg ctttaaataa taaagcaaga atacctgaat ggacaaatgg
     6781 gaagttttta ccaaaaagaa aagttgtaaa aattgagcaa ttcgaaaacg agattaagaa
     6841 ttgggaattc ccgttagtaa ttaagccggg agatgatctt ccgacagcag gtggctatgg
     6901 cgttatgatt tgttatcatg atgcagattt acaaaaagcg ataacgcgta ttaaagaggc
     6961 aactgctgaa acgaattcgc ttattatcga acagaagatt gaagaaaaag cgaattattg
     7021 cgttcagttt gcatactcgg aatcgctagg cattcaatat cttggtgcgg caacgcaatt
     7081 aacggataag tacggatttt ataacggaaa tgaaaatact actaatgtac cagagcacgt
     7141 tatagaagca ggtagacaaa ttatggaaaa cggtgtgaat caaggattct ttggtgtagc
     7201 tggttttgac ctactcgtcg atgaggatga taacgtttat gcgattgatt taaactttag
     7261 acaaaatggt tcaacgagca tgttattact tgctaacgag ttgaattcag gatatcaaaa
     7321 gttttatagt tatcattcaa aaggtgataa cacacatttc ttcaatacga ttttgaaata
     7381 tgtcaaagaa ggtagtttat acccgttatc ttattatgat ggtgattggt acggtgaaga
     7441 taaagttaaa tcaaggtttg gctgtatttg gcatggtgat tcaaaagaaa cagtactgga
     7501 gaatgaacgc gcatttttag ctgaacttga acactattag agttcggaac ataaggcgct
     7561 acaatgttgt gttgccagta gttgactgaa tatgcgtttg taacaagctt ttttcgattc
     7621 tagtcaacag taattaaatt tatgatatgg caatactttg taatactaat attaaatggc
     7681 gacttttatt tcactatgtt ataagagttg ccattttgtt gataaaggta tactaaaggt
     7741 tatcgttttg aaatttttag taactagata tgtttcgtgt tatagaccga atttgtgtat
     7801 acgtaaaatt taatgctatt gaatttttaa aatgaaaaac atgacattaa attgaattca
     7861 taatatgtct aattgactaa cttgttggag tcatttacta ttttatgtat gacatatttt
     7921 aaaaagtgag ggtcaagcat gtcttataaa gcatatccat tctttagaga tatattaata
     7981 aatgaatgta tttatttcgc ctctaaaaat aaaaaactag tacgcctaaa ttataaaagt
     8041 gaagcgtatg taggcgtttg gacagaagaa agtgtggccg tatcattttt aacaagtcgt
     8101 gatattccat ttgataaagt tgtaaaaatg gacgttgatc gttttgctac ttatgaatta
     8161 gatgaattgt ttgatgaaca agaccatatt attatgaatc aaacaatgga agaagaaggg
     8221 catctactaa acgttgtagc tgttacacaa gaagtgatga cggaattaga taaaattaga
     8281 atcaaagaat ttgtccaaga tgtagcgaaa tatgatgaag tatacggctt aactaaaaaa
     8341 ggtagtaagc agtttattct cattagtgaa aatgatagcg acgaaaaaaa gccgcatatt
     8401 atgcctgtat ggagtattaa aaacagagcg ttaaaagttc gagatgaaga ttttgaagag
     8461 tgtgatttaa ttacgattga aggttctgtt ttcggagaat ggctagatga acttagagat
     8521 gatcataaag ccgttgcgat agatttaaaa actggcgtgg ttggtacaat tgtttcagcg
     8581 caaaaattgt ctaatgaact tacattttaa ttgaattgag ttgtaagtaa ttaagtgata
     8641 ataaatggaa caatacgtat tcaaaacact tagaccataa aataaaaggc catttatata
     8701 gcgtttattt aaaacaacgc gcatataaat ggtctttttc tatttttcta aatataatgc
     8761 accaatagca cctgaaaatg cgccgttttc aacatagtac ggtttgcaac cgcgtaacac
     8821 agtataatct tccacaactt tgcgtaataa agcgttatta tgaaatgaag aaccgatata
     8881 aacgatattt tcagttttaa attcacgtgc aacagtaatg gccattgtcg taacaacttc
     8941 gccaacgaca ccaataacgg ctgctaattt attgctaggt gtaaaatcag catctaaatg
     9001 atgtagtaca tgaccaaaat tagctgctgt taaatcaccg ggaatgggtg gttcggtatc
     9061 tttataaata tgtctaacct ttaaatcgat agtgttacga tcaccgtgtt gtgccatgtc
     9121 agttaactgt ttataatcag tgatttgact tagtaaataa ccgagtcctt gaatcatgcc
     9181 tccacctgta ccgataccgc ctacacgacg ttgtgattgg ccgtcgaaat aatgtagtga
     9241 cgtaccggta ccaacatttg caaaaatata atctgctaag tcatggcctt gctcttttaa
     9301 caaaatacct agtccttgag atgcagcatc aaactctaca aaaatttgtg caggaatgtt
     9361 gatgttttca gcaatgacac ctgcattacc tccagttaag cataattttt caatttgctg
     9421 ttggtttaac cattccacaa cttgatcaat atttttagtt aattcagttt taaaagtacg
     9481 ttggttatct tgctcttgaa cgattttaat tagtgtaccg ccagcgtcaa tgccaacttt
     9541 cataagattc ccacctcatt attaatgtct atccttaaat aatagtatag taaaatgact
     9601 aaaaaacaag taataatagt aattattaac aaatttgatg ccattgtatt tcaacattgt
     9661 aagcgtatcg caattaatgt attacaaacg tagacgttaa gttatatata ttattttcta
     9721 ggaattttga agttgtatag gattgttagt tagtgacgca atattaaaag tagttcgtac
     9781 gcagtgtatt tgtaagtctc tgattaaaat gataagtaat gaggagtagc acattaattt
     9841 taaaattcaa aaaatataaa taagtaattt atttaactta gagcaaataa tggtatcgta
     9901 gtgaaataat aggtaaaata atatggggat tcatgcttca tatataaaaa gataggggtt
     9961 aaatatatgg ctaaagaact ttgttttgaa ggtatcactt taaaagcatt tgatgaacaa
    10021 tatcgttcag caattaatga ttttgacttg aatgaaagac aacaaatata ttcatcttta
    10081 cctaaagaag ttattgatga tgcaattaat gatgctgata ggattgctaa cgtagcaata
    10141 aacgataaaa atgaagtggt gggctttttt gtattacatc gttactatca gcatgaaggt
    10201 tatgatacac ctgaaaatgt cgtttatatt cgttcattat cgattaatga aaaatatcaa
    10261 ggttttggat atggcacgaa aataatgatg tcattgccgc aatatgttca aggtgtattt
    10321 cctgatttta atcatctata tctagtagta gatgcggaaa atgacaatgc ttggaacctt
    10381 tacgaacgtg ctggattcat gcatacagcg actaaagaag aaggaccaat tggcaaagaa
    10441 cgactatatt acttggactt agattcaaaa catgtttcgt cattaaagct tgaagaagaa
    10501 agtcgttcag aagtgaccaa tgtacatatc attaatttaa tgattgatgg ccaaaaggtt
    10561 ggctttatcg cattggagca gattggtgaa cgcatgaaca ttgctgctat tgaagtggat
    10621 aaatcatatc gctttaatgg tattggttca agtgcgctgc gacaattgcc aacttactta
    10681 agaaaaaact atgacaacct taatgtgatt acgatgattc tgtttggaga gaataatgat
    10741 tttaaaccat tatgtctaaa tagtaatttc gttgaaatcg aacaaactga tgattatgtc
    10801 gtttttgaaa aatatttaaa ttactaacag tgattgcgaa atatgatatt gtcatttata
    10861 atttagtttt gttactatat ataaatgaat tcagacgtat aaatttagat tatatccttc
    10921 gaaaggaagt attgggcaat gaaaattcaa gattatacaa aacaaatggt tgatgaaaaa
    10981 tcatttattg atatggctta tacattattg aatgataaag gcgaaacaat gaacttatat
    11041 gatatcatcg atgaatttag agcgttaggt gattatgagt acgaagaaat tgaaaatcgt
    11101 gttgtacaat tttacacgga tttaaacaca gatggtcgtt ttttaaatgt tggagaaaat
    11161 ttatggggat tacgtgattg gtattcggta gatgatattg aagagaaaat cgcaccaact
    11221 attcaaaaat tcgatattct ggatgcagat gatgaagaag atcaaaactt aaaattattg
    11281 ggcgaagatg aaatggatga cgacgatgat attccagctc aaacagatga tcaagaagaa
    11341 ctaaatgatc cagaagatga gcaggttgaa gaagaaatca atcattcgga tatagtcatt
    11401 gaagaagatg aagatgaact agacgaagac gaagaagtgt ttgaagacga agaagacttc
    11461 aacgattaat tttttgtttg acttttagtt gaaagatgat aaaattttat tcgggctcct
    11521 ttaaatagga cacgtgtata aaatttatac gctcccctta cagaatttgt gagagggagc
    11581 gtttttttat ttaattgagt aaatcaagaa atgataacgc aaaaatcaaa gttgtaaatg
    11641 atatacatag tgacatagca gtatggaaac ggtaagtaaa cagaatttaa ttttgtcgaa
    11701 ttcgacaaat aaacaacttg aatgagcttg ctttaatgtt atgttttacg taatttttac
    11761 aattgatgag gaagcattcc ctttaataat taggaggtca agacatgaca aaatttattt
    11821 ttgtaacagg tggcgtagtt tcatcattag ggaagggtat tacagcatct tctctaggta
    11881 gattattaaa agatagaggt ctaaatgtaa caattcaaaa attcgatcca tacttaaatg
    11941 ttgacccagg tacaatgagt ccttatcaac atggtgaagt attcgtaacg gatgatggtg
    12001 cagaaactga cctagactta ggacattacg aaagatttat cgatattaat ttaaacaagt
    12061 tttcaaatgt gacagccggt aaagtgtatt cacacgtatt gaaaaaagaa cgtcgtggtg
    12121 attacttagg tggaacagtt caagttattc cgcatattac aaatgaaatt aaagaacgtt
    12181 tattacttgc aggggaaagt acgaatgcag acgttgttat cactgaaatt ggcggtacaa
    12241 caggtgatat tgagtcatta ccgtttattg aagcgattcg tcaaattcgt agcgatttag
    12301 gtagagaaaa tgttatgtat gttcactgta cattactgcc ttatattaaa gctgctggag
    12361 aaatgaaaac gaagccaaca caacatagtg ttaaagaatt acgaggctta ggtattcaac
    12421 cagacttaat cgttgtaaga actgaatatg aaatgacaca agatttaaaa gataaaattg
    12481 cattattctg tgacattaat aaagaaagtg ttattgaatg tcgtgatgca gactctttat
    12541 acgaaattcc attacaatta agccaacaaa atatggatga tatcgttatt aaacgtttac
    12601 aattaaacgc gaaatatgaa acacagcttg atgaatggaa acagttgtta gatatcgtta
    12661 ataatttaga tggtaaaatt acaattggtt tagtaggtaa atatgttagc ttacaagatg
    12721 catatttatc agttgttgaa tcattgaaac atgctggata tccttttgcc aaagatattg
    12781 acattagatg gattgattca agtgaagtaa cagatgaaaa tgcagccgaa taccttgcag
    12841 atgtcgacgg tattttagta ccaggtggat ttggtttccg tgcaagtgaa ggtaaaatta
    12901 gtgcaattaa gtatgctaga gaaaacaatg taccattctt tggtatttgt ttaggaatgc
    12961 aacttgcaac agttgaattt tcaagaaacg tattaggcct tgaaggcgca cattcagctg
    13021 aattagaccc agcaacacca tacccaatta tagatttatt accagaacaa aaagatatcg
    13081 aagatttagg tggtacatta cgcttaggct tatatccatg ttcaattaaa gaaggcacat
    13141 tggcacaaga tgtttatggt aaagcggaaa ttgaagaaag acatcgtcat cgttatgaat
    13201 ttaataatga ctatagagaa caattagaag caaatggtat ggtaatttct ggtacaagtc
    13261 cagatggacg tttagtagaa atggtagaga ttccgacaaa tgatttcttt attgcttgtc
    13321 aattccaccc agaattctta tctagaccaa atcgtccgca cccgattttt aaatcattta
    13381 ttgaagcttc gttaaaatat caacaaaata aataaatttg ctaataaaac cggtactttc
    13441 attgttaaac attgaaagta ccggtttttc atataatttt aatattatgt tagtgacaag
    13501 gtatgaaata acaatagtga cttttataat tctaagtctc ttgtcatttc aatcatttgt
    13561 gtataaatgt catagtatac ataattcaat gccatcgcat gtggttggac aatcttatcg
    13621 taatcttcag tgtagactat aggtcttggt gtagataaat cgataaaatg tacgagatga
    13681 tcagggaaat catctgtttt aggtttgttg cttattaaga ccacatcgat atctaagtcg
    13741 ataagttttt gaatatctaa tgcaacttga tcattataaa atggtgcgaa taataataca
    13801 cgatcagttg agtcaatttc tttaaagtct ttaatagcgt caagttttcg gctagatttt
    13861 aaacgctcat cactatgtag aatgaaactt tcgaaaaact gtaaatcatc ataacctttt
    13921 acataaacat aaccttcgcc accaattgct tgaattaaac attgggcggc catttgaatt
    13981 tctaaagatt gtttttctag cctattaaag atacctatta gttgtgtgtt taagattttt
    14041 gacatcttta tcctccaatc tacttataaa atattgtaat taatgactac atattatgca
    14101 acggcttaaa ttgtataaaa atgtatacgt ttgcatttag tataactatc gcatttttca
    14161 aaaaatacac atttaatctg cagtatttca atgcattgac gctatttttt tgatataatt
    14221 actttgaaaa atacgtgcgt aagcactcaa ggaggaactt tcatgccttt agtttcaatg
    14281 aaagaaatgt taattgatgc aaaagaaaat ggttatgcgg taggtcaata caatattaat
    14341 aacctagaat tcactcaagc aattttagaa gcgtcacaag aagaaaacgc acctgtaatt
    14401 ttaggtgttt ctgaaggtgc tgctcgttac atgagcggtt tctacacaat tgttaaaatg
    14461 gttgaagggt taatgcatga cttaaacatc actattcctg tagcaatcca tttagaccat
    14521 ggttcaagct ttgaaaaatg taaagaagct atcgatgctg gtttcacatc agtaatgatc
    14581 gatgcttcac acagcccatt cgaagaaaac gtagcaacaa ctaaaaaagt tgttgaatac
    14641 gctcatgaaa aaggtgtttc tgtagaagct gaattaggta ctgttggtgg acaagaagat
    14701 gatgttgtag cagacggcat catttatgct gatcctaaag aatgtcaaga actagttgaa
    14761 aaaactggta tcgatgcatt agcgccagca ttaggttcag ttcatggtcc atacaaaggt
    14821 gaaccaaaat taggattcaa agaaatggaa gaaatcggtt tatcaactgg tttaccatta
    14881 gtattacacg gtggtactgg tatcccaact aaagatatcc aaaaagcaat tccatttggt
    14941 acagctaaaa ttaacgtaaa cactgaaaac caaatcgctt cagcaaaagc agttcgtgac
    15001 gttttaaata acgacaaaga agtttacgat cctcgtaaat acttaggacc tgcacgtgaa
    15061 gctatcaaag aaacagttaa aggtaaaatt aaagagttcg gtacttcaaa ccgcgctaaa
    15121 taattaatat ttagtcttta agttattaat aacgtaggga tattaatttt aaaagaagca
    15181 gacaaaatgg tgtttgcttc ttttttatgt tgtataagta ataaataaaa cagtttgatt
    15241 ttaaaatgaa agcgtaaaaa tggtaaaata tatcaaaatt gattgtgata taattataag
    15301 gaaaatgagc aatttatgaa aaaagtttac gtgaaaaccg tagaattaaa actaaataat
    15361 tgtcaaaaca acgtcaatat ttagttgaat aatcagactt tagaccatgg tcaagtgggg
    15421 aagaacagca tatattagta aaggtgaatg atttgttatt aatcaatcga aaatagaaag
    15481 acaagatttt aacgattaaa ataaactatt ttacaaataa agtaaaatta atttataatg
    15541 ctaataatgc aaaaaattaa aaagtaatgg acaaaggaga taaatgatat ggctcaagag
    15601 gtaataaaaa taagaggtgg acgcacacta aatggggaag ttaatatcag tggcgcgaaa
    15661 aacagtgctg tagctatcat tcctgcaaca ttattagctc aaggacatgt gaaattagaa
    15721 gggttaccgc aaatctctga tgttaaaact ttagtgagtt tattagaaga tttaaatatt
    15781 aaggcatcac taaatggtat ggaattagaa gttgatacaa ctgaaataca aaatgctgca
    15841 ttaccaaata ataaagttga atcattacgt gcttcatact atatgatggg tgccatgtta
    15901 ggtagattta aaaaatgtgt gattggttta ccgggaggat gtccacttgg gccacgtcca
    15961 attgatcaac atattaaagg atttaaagct ttaggtgctg aaattgatga atcaagtacg
    16021 acatcaatga aaatcgaagc taaagagtta aaaggtgcac atatttttct agatatggtt
    16081 agcgtaggtg caacaattaa cattatgcta gctgcagttt atgcaacagg acaaactgta
    16141 attgaaaatg ctgctaaaga accggaagta gttgatgttg ctaacttctt aacaagtatg
    16201 ggagctaata ttaagggagc gggtacatca acaattaaaa tcaatggcgt caaagaatta
    16261 catggttctg agtatcaagt tatacctgat agaattgaag caggcacata tatgtgtatc
    16321 gctgcagcat gtggagaaaa tgttgtatta aataatattg ttccgaaaca tgtagaaaca
    16381 ttaactgcta aatttagtga attaggtgtg aatgttgatg taagagacga aagaattcgc
    16441 atcaataata atgcgccata tcaatttgtt gatattaaaa cactagtata tccaggtttt
    16501 gctactgatt tgcaacagcc tattacacca ttattattta tggcaaatgg tccttcattt
    16561 gtaactgata cgatttatcc ggagcgtttt aaacatgttg aagaattaaa gcgcatgggt
    16621 gcaaatattg aagttgacga aggcacagca acgataaagc catcaacatt acatggtgct
    16681 gaagtttatg ctagcgattt aagagcagga gcttgtttaa ttattgctgg tttaattgct
    16741 gagggtgtaa ctacaattta caatgtaaaa catatttata gaggttatac agatattgta
    16801 gaacacttaa aagctttagg tgcagatatt tggacggaaa ctgtatagat ttatcttgtt
    16861 ttgtggtata ataattatac tgattaaaaa agggtgttcg tttacattct aaatttattg
    16921 atgagaggtg aagatgatgg aagtatgtcc gtatctcgaa gaaactttta aaatacttgg
    16981 tagaagttgg aatggattaa tcattaatta tctctcaaga tgtaatgact gttcagcaca
    17041 cttttccgat atgaaaagag atttgaaaac aataacacca cgtgctttaa gtcttaagtt
    17101 gtcagagctt gcacaatggg agttagttga gaagcaaatc atttctacga gtccagtaca
    17161 aattatttat gtgctaactg aaaaaggtaa agcgttagca gaggctttac atccaattga
    17221 agcatgggcg caatcatatg tcgatttaac agatcaacgt actgctaaat aattacatga
    17281 cgtttaaaca tagcattgat tataactatt tctaagtctt cgcattattt gcgatgatgt
    17341 gggaatagtt atttttattt aaaaatataa aaaaatagat gcagcaaaat tttaaagcat
    17401 tttattttga acatattaaa agggagcgta tcataatgga atgtaatgtt tatatcgtat
    17461 gcattacgga taaataatat ataaatcatt cttgaggagt gaaagaataa tgagagacta
    17521 cacaaagcaa tacattaatg gcgaatgggt agaaagtaat agtaatgaaa cgatagaagt
    17581 tataaatcca gcaaccgaag aagtaatcgg gaaagttgct aaaggcaata aagctgatgt
    17641 tgataaagct gtcgaggcgg cagacaatgt ttatttagag ttccgtcata catctgtgaa
    17701 agaaagacaa gcgttattag ataaaattgt aaaagaatat gaaaacagaa aagacgatat
    17761 tgtacaagct attacggatg aattaggtgc tcctttatca ttatctgagc gtgtccatta
    17821 tcaaatggga ctaaaccatt ttgttgcagc gagagacgca ttagataact acgaatttga
    17881 agaacgccgc ggagatgatt tagttgttaa agaagcaatc ggtgtatctg gcttaattac
    17941 accgtggaac ttccctacaa accaaacatc attaaaatta gcagcagcat ttgcggctgg
    18001 tagtccagtt gtacttaaac catctgaaga aacaccattt gcagctgtta ttttagctga
    18061 gatttttgat aaagtcggtg ttcctaaagg tgtatttaac cttgttaatg gtgatggtgc
    18121 tggcgttggg aatcctttat ctgagcatcc taaagtacgc atgatgtcat ttacaggatc
    18181 aggccctact ggttctaaaa ttatggaaaa agctgctaaa gattttaaaa aggtatcatt
    18241 agagcttggt ggcaaatcac catacatcgt cctagatgac gtagatatta aagaagcggc
    18301 taaagcaaca acaggcaaag ttgttaataa tactggtcaa gtatgtacag ctggtacacg
    18361 agttttagtg cctaaaaaaa ttaaagatgc attcttagct gaattaaaag aacaatttag
    18421 ccaagtgcgt gtcggtaatc caagagaaga tggtacacaa gtaggcccta tcattagtaa
    18481 aaaacaattt gatcaagtac aaaattatat taataaaggt attgaagaag gtgctgaatt
    18541 attttatggt ggtcctggta aaccagaagg acttgaaaaa ggatactttg cacgtccgac
    18601 aatttttatt aatgtggata atcaaatgac gatagcacaa gaagaaattt ttgggccagt
    18661 aatgtcagtt atcacttata acgatttaga tgaagcgatt caaattgcaa atgatacaaa
    18721 atatggtttg gcaggatatg ttattggtaa ggacaaagaa acattgcata aagtagctcg
    18781 ttctattgaa gcaggtacag tagaaataaa cgaagcaggt agaaagccag atttaccatt
    18841 tggtggctat aaacaatctg gtttaggtcg tgaatggggc gattatggta ttgaagagtt
    18901 cttagaattg aaatctatag ctggatattt taaataaatt tgatttttga attaaaaatc
    18961 gcaataaaac agtgcacatg actaattaag ttttgtgtac tgttttaatt ttgcaatttt
    19021 tataaataga ttttgtaatt aaaataaaaa tttgctatag ttattcatgt atttaaaagg
    19081 ttggggatta gcataatggg attgtgctag cacagttatt tatgcattgt catgcctatc
    19141 tattacttac taactaaaaa ataatgaaat gggtgtaaac tatatgcctg aaagagaacg
    19201 tacatctcct cagtatgaat cattccacga attgtacaag aactatacta ccaaggaact
    19261 cactcaaaaa gctaaaactc ttaagttgac gaactatagt aaattaaata aaaaagaact
    19321 tgttctagct attatggaag cacaaatgga aaaagatggt aactattata tggaaggtat
    19381 cttagatgat atacaaccag atggttatgg ttttttaaga acagtgaact attctaaagg
    19441 ggaaaaagat atttatatat ctgctagcca aattcgtcgt tttgaaatta aacgtgggga
    19501 taaagtaact gggaaagtta gaaaacctaa agataacgaa aaatattatg gcttattaca
    19561 agttgacttt gtcaatgacc ataacgcaga agaagtgaag aaacgtccgc atttccaagc
    19621 tttgacacca ctttatccag atgagcgtat taaattagag acagaaacac aaaattattc
    19681 aacgcgcatc atggatttag taacaccgat tggtttaggt caacgtggtt taatagtggc
    19741 gccacctaaa gcaggtaaaa catcgttatt aaaagaaata gcgaatgcaa tcagtacgaa
    19801 caaaccagat gcaaagctat ttattttgtt agttggcgag cgtcctgaag aggtaacaga
    19861 tttagaacgc tcagtagaag ctgctgaagt cgttcattca acgtttgacg aaccaccaga
    19921 acaccatgtt aaagtagctg aattattact tgaacgtgca aagcgtttag tagaaattgg
    19981 ggaagatgtc attattttaa tggattctat aacgagatta gcacgcgctt ataacttagt
    20041 tattccacca agtggtcgta cattatcagg tggtttagat cctgcatctt tacacaaacc
    20101 aaaagcattc ttcggtgcag cgagaaatat tgaagcgggt ggaagtttaa caatacttgc
    20161 aactgcatta gttgatacgg gttcacgtat ggacgatatg atttacgaag aatttaaagg
    20221 aacaggtaac atggagttac atttagatcg taaattgtct gaacgtcgta tcttccctgc
    20281 aattgatatt ggcagaagtt caacgcgtaa agaagaattg ttgataagta aatctgaatt
    20341 agacacatta tggcaattaa gaaatctatt cactgactca actgacttta ctgaaagatt
    20401 tattcgcaaa cttaaaaggt ctaagaataa tgaagatttc ttcaagcagc tacaaaagtc
    20461 tgcagaagaa agtactaaaa cgggtcgacc tataatttaa taaacattat ataggggctt
    20521 gcgttttgaa ttaattacct ttataattac acagtattgg gtaaaaactc acaaataact
    20581 ctgttccaga tggttcaggg caaaggagct gaaaataatg aaacaaggaa ttcatcctga
    20641 gtatcaccaa gtaatctttt tagatactac tacaaacttc aaattcttaa gcggttctac
    20701 aaaaacatct tcagaaatga tggagtggga agatggaaaa gaatacccag ttattcgttt
    20761 agatatttca tctgattcac atccattcta cactggacgt caaaaattcg ctgctgcgga
    20821 tggacgtgtg gaacgtttca acaaaaagtt tggtcttaaa tcaaacaact aatatagtaa
    20881 tcgttaagct gtctgcatcc tataatggaa ttgtagacag cttttttatt tataagcgta
    20941 gtaagggtat atacttatga ctgtgttcaa agaagtgact caataccatg ttgttaatac
    21001 ataatgataa atttccccct ttattaaagt ttgtattgag tagaaacacc gcacgaataa
    21061 gaagcaacaa attagaatag ctgtatataa atatgcaaga caaatttata aaataattgt
    21121 tcatgtacaa tatcgatata attatgaaaa agacatttat gaatatttga aatatgatat
    21181 aataaagcga ttatgttttg aaaaaggtgg aactctcaat gtatgaaact taccattccg
    21241 gttggataga atgtattaca ggtagtatgt ttagcggtaa atccgaagaa ctaataagac
    21301 gtctaagaag gggtatatac gctaaacaaa aagtcgttgt atttaaaccg gcgattgatg
    21361 atcgatatca taaagaaaaa gtggtctctc ataatggtaa tgccattgaa gcgattaata
    21421 tttctaaagc aagtgaaatt atgacgcacg acttaactaa tgttgatgtt ataggaatag
    21481 acgaagtgca attttttgac gatgaaattg taagtatagt agaaaaacta tcagcagatg
    21541 gacaccgtgt tattgttgct ggattagata tggattttag gggcgaaccg ttcgaaccaa
    21601 tgcctaaatt gatggctgtc agtgaacaag taacgaaact tcaagcagtt tgtgctgttt
    21661 gtggttcttc atctagtaga acgcaacgat taataaatgg taaaccagct aaaatcgatg
    21721 atccgattat cttagtaggt gccaatgaaa gttatgagcc acgttgtcgc gcgcaccata
    21781 tcgtagcacc tagtgataat aataaggagg aattataagt gtttgatcaa ttagatattg
    21841 tagaagaaag atacgaacag ttaaatgaac tgttaagtga cccagatgtt gtaaatgatt
    21901 cagataaatt acgtaaatat tctaaagagc aagctgattt acaaaaaact gtagatgttt
    21961 atcgtaacta taaagctaaa aaagaagaat tagctgatat tgaagaaatg ttaagtgaga
    22021 ctgatgataa agaagaagta gaaatgttaa aagaggagag taatggtatt aaagctgaac
    22081 ttccaaatct tgaagaagag cttaaaatat tattgattcc taaagatcct aatgatgaca
    22141 aagacgttat tgtagaaata agagcagcag caggtggtga tgaggctgcg atttttgctg
    22201 gtgatttaat gcgtatgtat tcaaagtatg ctgaatcaca aggattcaaa actgaaatag
    22261 tagaagcgtc tgaaagtgac catggtggtt acaaagaaat tagtttctca gtttctggta
    22321 atggcgcgta tagtaaattg aaatttgaaa atggtgcgca ccgcgttcaa cgtgtgcctg
    22381 aaacagaatc aggtggacgt attcatactt caacagctac agtggcagtt ttaccagaag
    22441 ttgaagatgt agaaattgaa attagaaatg aagatttaaa aatcgacacg tatcgttcaa
    22501 gtggtgcagg tggtcagcac gtaaacacaa ctgactctgc agtacgtatt acccatttac
    22561 caactggtgt cattgcaaca tcttctgaga agtctcaaat tcaaaaccgt gaaaaagcaa
    22621 tgaaagtgtt aaaagcgcgt ttatacgata tgaaagttca agaagaacaa caaaagtatg
    22681 cgtcacaacg taaatcagca gtcggtactg gtgatcgttc agaacgtatt cgaacttata
    22741 attatccaca aagccgtgta acagaccatc gtataggtct aacgcttcaa aaattagggc
    22801 aaattatgga aggccattta gaagaaatta tagatgcact gactttatca gagcagacag
    22861 ataaattgaa agaacttaat aatggtgaat tataaagaaa agttagatga agcaattcat
    22921 ttaacacaac aaaaagggtt tgaacaaaca cgagctgaat ggttaatgtt agatgtattt
    22981 caatggacgc gtacggactt tgtagtccac atgcatgatg atatgccgaa agcgatgatt
    23041 atgaagttcg acttagcatt acaacgtatg ttattaggag agcctataca gtatatagtt
    23101 ggctttgcct cattttatgg tagaacgttt gatgtaaact caaattgttt gataccaaga
    23161 cctgaaactg aagaagtaat gttgcatttc ttacaacagt tagaagatga tgcaacaatc
    23221 gtagatatcg gaacgggtag tggtgtactt gcaattactt tgaaatgtga aaagccggat
    23281 ttaaatgtta ttgctactga tatttcactt gaagcaatga atatggctcg taataatgct
    23341 gagaagcatc aatcacaaat acaattttta acaggggatg cattaaagcc cttaattaat
    23401 gaagatatca agttgaacgg cttgatatct aatccaccat atatagatga aaaagatatg
    23461 gttacgatgt ctccaacggt tacgagattc gaaccacatc aggcattgtt tgcagataac
    23521 catggatatg ctatttatga atcaatcatt gaagatttac ctcacgttat ggaaaaaggc
    23581 agcccagttg tttttgaaat tggttacaat caaggtgagg cacttaaatc aataatttta
    23641 aataaatttc ctgacaaaaa aatcgacatt attaaagata taaatggcca cgatcgaatc
    23701 gtctcattta aatggtaatt agaagttatg cctttgctat gattagttaa gtgcatagct
    23761 ttttgcttta tattatgata aataagaaag gcgtgattaa gttggatact aaaatttggg
    23821 atgttagaga atataatgaa gatttacagc aatatcctaa aattaatgaa ataaaagaca
    23881 ttgttttaaa cggtggttta ataggtttac caactgaaac agtttatgga cttgcagcaa
    23941 atgcgacaga tgaagaagct gtagctaaaa tatatgaagc taaaggccgt ccatctgaca
    24001 atccgcttat tgttcatata cacagtaaag gtcaattaaa agattttaca tatactttgg
    24061 atccacgcgt agaaaagtta atgcaggcat tctggccggg ccctatttcg tttatattgc
    24121 cgttaaagcc aggctatcta tgtcgaaaag tttctggagg tttatcatca gttgctgtta
    24181 gaatgccaag ccattctgta ggtagacaat tattacaaat cataaatgaa cctctagctg
    24241 ctccaagtgc taatttaagt ggtagacctt caccaacaac tttcaatcat gtatatcaag
    24301 atttgaatgg ccgtatcgat ggtattgttc aagctgaaca aagtgaagaa ggattagaaa
    24361 gtacggtttt agattgcaca tcttttcctt ataaaattgc aagacctggt tctataacag
    24421 cagcaatgat tacagaaata cttccgaata gtatcgccca tgctgattat aatgatactg
    24481 aacagccaat tgcaccaggt atgaagtata agcattactc acccaataca ccacttacaa
    24541 ttattacaga tattgagagc aaaattggaa atgacggtaa agattggtct tctatagctt
    24601 ttattgtgcc gagtaataag gtggcgttta taccaagtga agcgcaattc attcaattat
    24661 gtcaggatga caatgatgtt aaacaagcaa gtcataatct ttatgatgtg ttacattcac
    24721 ttgatgaaaa tgaaaatatt tcagcggcgt atatatacgg ctttgagctg aatgataata
    24781 cagaagcaat tatgaatcgc atgttaaaag ctgcaagtaa tcacattatt aaaggatgtg
    24841 aactatgaag attttattcg tttgtacagg taacacatgt cgtagcccat tagcggaaag
    24901 tattgcaaaa gaggttatgc caaatcatca atttgaatca agaggtatat tcgctgtgaa
    24961 caatcaaggt gtttcgaatt atgttgaaga cttagttgaa gaacatcatt tagctgaaac
    25021 gaccttatcg caacaattta ctgaagcaga tttgaaagca gatattattt tgacgatgtc
    25081 gtattcgcac aaagaattaa tagaggcaca ctttggtttg caaaatcatg ttttcacatt
    25141 gcatgaatat gtaaaagaag caggagaagt tatagatcca tacggtggaa caaaagaaat
    25201 gtatgtacat acctatgaag aacttgtaag tttaatttta aaattaaaag atattatttg
    25261 ctagtaggaa ttctgtataa ttagtatatg ttaacagtta aataagttat aatagaggag
    25321 tgtagcactt tgctatattc cttttttatt tttaggaagg gtgggctttt atgaaagatt
    25381 tgacaatgtt attagacgaa ttaaaagata tgtccttttt taataaagga gatatatgtt
    25441 taattggatg ttcaacatcg gaagtcattg gcgaaaaaat agggactgtc ggttcgatgg
    25501 aagttgcaga aactattttt aatgcattag atgttgtgag taaagagaca ggcgttactt
    25561 ttgcttttca aggatgtgaa catatcaaca gagcgataac aatagaaaaa tctcagtata
    25621 atccattaac aatggaagaa gtttccgtcg ttccagatgt acatgctgga gggagtttgg
    25681 caacatacgc ttttcagcat atgaaggatc cgatcgtggt cgaacatatt actgttcctt
    25741 gtggaattga tattggtcaa acgttaatag gcatgcatat taaacatgta tgtgtacctg
    25801 taaggacctc agtaaagcaa gtaggacagg caattgtaac tattgccaca tctaggccga
    25861 aaaagattgg tggcgaacgt gctaaatacc aataagtttt tttgaaagaa ggggagattt
    25921 tatgtcttat atcaccaagc aagataaagt tatcgcagaa gcaatcgaga gagaatttca
    25981 gagacaaaat agcaacatag agttaatcgc atcggaaaat tttgtatcgg aagcggttat
    26041 ggaagcacaa ggttcagtgt tgactaataa gtatgctgaa ggctatccag gacgccgata
    26101 ttatggtggc tgtgagtttg tagatgttac tgaaagcatc gcaattgatc gtgctaaagc
    26161 attgtttgga gctgaacatg tcaatgttca accacattca ggttcacaag cgaacatggc
    26221 tgtttactta gttgcattag aaatgggcga cacagtttta ggtatgaatt tgagtcatgg
    26281 tggtcacttg acacatggag cgcctgttaa ttttagtggt aaattctaca atttcgttga
    26341 atatggagta gataaagaca cagaacgaat caattatgat gaagttcgta aattagcgtt
    26401 agagcataag cctaagctta ttgtggcagg agcatcagca tattcaagaa caattgactt
    26461 caaaaagttt aaagaaatcg cagatgaagt aaacgctaag ttaatggtag acatggcaca
    26521 tattgcagga ttagtagcgg caggtttaca tccaaatcca gtagaatatg ctgattttgt
    26581 aacaactaca acacacaaaa cattacgcgg accacgtggt ggtatgattt tatgtaagga
    26641 agaatataaa aaagacatag ataaaacaat tttccctggt attcaaggtg gacctcttga
    26701 gcatgttatt gcagcaaaag cagttgcttt tggagaagcg ttagaaaata atttcaaaac
    26761 gtatcaacaa caagtggtta aaaacgcaaa agttcttgca gaagcattaa ttaatgaagg
    26821 atttagaatt gtttctggcg gtacagataa tcacttagta gctgttgatg taaaagggtc
    26881 tataggactt actggtaaag aagctgaaga gactttagat tcagttggta tcacatgtaa
    26941 caaaaatacc attccgttcg atcaagaaaa accttttgta acgagtggta tacgtttagg
    27001 tacacctgct gcaacaacgc gtggatttga tgaaaaagct tttgaggaag ttgcaaaaat
    27061 catcagttta gcattgaaaa atagtaaaga tgaagaaaaa ttacaacaag ctaaagaacg
    27121 cgttgcgaaa ttaacagctg aatatcctct atatcaataa acatacacaa agataggagt
    27181 aatttttatg agtaaagtac acgttttcga tcatccatta attcaacaca aactaagcta
    27241 tattagagat gtaaatacag gtactaaaga atttagagaa cttgtagacg aagttggtat
    27301 gttgatggca tatgaagtaa ctagagactt agagcttcag gatgttgata ttgaaacgcc
    27361 tgtaactaaa atgacggcta aacgattagc tggtaaaaaa ttagctatcg ttccaatttt
    27421 aagagctggt ttaggtatga ctgatggtat tttaagttta gtgcctgcag ccagagttgg
    27481 acatatcggt ttgtatcgag atcccgaaac attaaaggct gtagagtatt ttgctaagtt
    27541 acctcaagat attactgaaa gacaaatcat tgttgttgat cctatgttag caacaggcgc
    27601 atcagcaatt gaagctatta cttcattgaa gaaacgtggc gctaaaaata ttcgcttcat
    27661 gtgtttaatt gcagcaccag aaggcgtaga aaagatgcat gaagcacatc cagatgtaga
    27721 tatttatatt gctgcacttg atgaaaagct aaatgataaa gcatatatca caccagggtt
    27781 aggcgacgct ggagatagat tgtttggtac taaatagaag aatcggagat aagtagtttg
    27841 atgaaaaaga ttatgaccat atttggaaca agacccgaag ccataaagat ggctccttta
    27901 gttaaagctt tggaacaaga aaaaatgctc gagccaattg ttgttgttac agcacagcat
    27961 agagaaatgc ttgattcagt attaagtacc tttgaaatca aaccaaaata cgatttgaat
    28021 attatgaaat caggacagac actttctgag ataacttcta aatcaatcac tcaattagaa
    28081 caagtgattc aattagaaaa accagacatg gtacttgtac atggtgatac gatgacgaca
    28141 tttgcaggtg gtttagcggc attttataac caagtgccga ttggacacgt tgaagcaggg
    28201 cttagaagtt atgataaata ttcacctttt ccggaagagg tgaatagaca attagttggt
    28261 gtattagctg acttacattt tgcacctact aaaaatgctg catcgcattt gttaagtgaa
    28321 gggaaatatt cagaaagtgt tgttgtcact ggtaatactg cgattgatgc tatgaaatat
    28381 acagttgatg acaattataa atctaacatc atggataaat accatgacaa aaaattcatt
    28441 cttatgacag cacaccgacg agaaaatatc gggaaaccca tggaaaatat atttaaagcg
    28501 gttagacgct taatagatga gtatactgat ttagcactgg tatatccaat gcataaaaac
    28561 ccgaaagttc gagaggtggc tcaaaagatt ttaggtagtc atgacagaat tgaattgatt
    28621 gaacctttgg acgtagtaga tttccataat tttgctaaaa aatcttattt tattttgaca
    28681 gattcaggtg gaattcagga agaagcgcca tcatttaata agccagtttt agttttgcga
    28741 agcgttacag aacgtcctga aggtgttgaa gctggcacat taaaagtcat tggaacaaat
    28801 aagcaaaatg tgtatcaagc tgcaaaagaa ttaatcgatg atgagagatt atatcatcaa
    28861 atgtctgaag catctaatcc ttacggtgat ggatttgctt cagaaagaat agttaatcat
    28921 attaagtatt atttgaattt aatcactgaa aaaccaagcg atttctgagg aaaatagact
    28981 ttttgtctaa ttgcgtctaa attgtgtcta actatgctta tttttttaca acaatttgaa
    29041 tagaattgac accacaaatc gcctatatta tcatttagtt tgtatgagtg gaacggtttt
    29101 catgattgat atcagtcaca cggaggcata taaaatgagt cgtttctaca acatttttaa
    29161 acagttcatt caatattatt tttatctaat aataatattg ggaggattat acctttatac
    29221 acaccatgca tttatcttag gattaatcat tggtgttact ggttctgcaa tcaacacatg
    29281 tatttttgaa tgctatttag ctaaagctaa aagaccagac actatgcata tttcaactgg
    29341 aaatatgtgg cgatacttag ttgcaattat tgcctgtatg atttggtacc ttaataaagc
    29401 gcatgtaagt atcatcggta taattattgg tttaatgatt tcatatgttg tagttatcat
    29461 acgtccttta ctaaaggtga gcaaataaat taagaaagag gtgagattat ggatcacaaa
    29521 tccccgctcg tgagttggaa tttattcggt tttgatatcg ttttcaattt atcaagtata
    29581 ttgatgatac ttgttacggc gtttcttgtt tttctacttg ctatcatttg tacgcgtaat
    29641 ttgaaaaaaa gaccaactgg caaacaaaat ttcgttgaat ggatttttga tttcgtgagg
    29701 ggaatcattg aaggtaacat ggcttggaaa aaaggtggtc aattccactt cttagcagta
    29761 acgctgattc tgtacatttt tatagctaat atgttaggtc ttccgttttc tatagtaacg
    29821 aaagatcaca cattgtggtg gaaatcaccg acagctgatg caacagtgac tttaacgttg
    29881 tctacaacga taatactgtt aactcacttt tatggaatta aaatgcgtgg tacgaaacaa
    29941 tatcttaaag gttatgtaca gccgttttgg ccattggcaa ttattaatgt tttcgaagag
    30001 ttcacttcaa cattaacgct tggtctgcgt ttgtacggta acatatttgc aggtgagata
    30061 ctattaacat tacttgctgg cttattcttt aacgaaccag catggggttg gattattagt
    30121 atcccaggat taattgtttg gcaagcattt tcaatatttg taggaacaat ccaagcatat
    30181 atctttatta tgctttcgat ggtttatatg tcacataaag tggcagatga acactaaaaa
    30241 tttcaataat tatatacaat cacaggagga aattaaatta tgaatttaat cgcagcagca
    30301 atcgcaattg gtttatcagc attaggagca ggtatcggta acggtttaat cgtttcaaga
    30361 acagttgaag gtgtagcacg tcaaccagaa gcacgtggtc aattaatggg tatcatgttc
    30421 attggtgtag gtttagttga ggcattacct atcatcggtg tagtaattgc attcatgaca
    30481 tttgctggat aattaacaga taaaagaggt cgggacaaag cgcataggac ataattcatg
    30541 atgcatatat agtaatatct ttgaacttta ttaaatagtt gagatatgaa tgcaccatgc
    30601 ctatcgcata aattcagtag gtcctaacct cgtcgttttt tctatataac actagcgatt
    30661 attttaatga aaggagtgtc atgaacccgt gactgaaaca gctaacttat tcgttcttgg
    30721 tgcagctgga ggcgttgagt ggggtactgt gattgtacag gtcctaactt tcatcgtgtt
    30781 acttgcgtta cttaaaaagt tcgcatgggg tccattgaaa gatgtaatgg ataaacgtga
    30841 aagagatatt aacagagata tcgatgacgc agaacaagct aagttaaatg cacagaaact
    30901 tgaagaagaa aataaacaaa aacttaaaga aacacaagaa gaagttcaaa agattttaga
    30961 agatgctaag gttcaagcac gtcaacagca agaacaaatt attcatgaag caaacgtacg
    31021 tgcaaacggt atgattgaaa cagcacaaag tgaaatcaat agccaaaaag aacgtgccat
    31081 tgcagatatt aataatcaag tatctgaact atcagtgtta attgcttcta aagttcttag
    31141 aaaagaaatt tctgaacaag accaaaaagc attggttgac aagtatctaa aagaggcagg
    31201 cgataaataa tggtaaaagt agctaacaag tatgctaaag cattatttga cgtgtcatta
    31261 gatacaaata atttagagac tattaatgaa gaattaacag ttataaatga agcagtaaaa
    31321 gataaaattg agcaattgaa aatggttgac agtaatccaa ctcaaacagc agaacaacgt
    31381 cgcgagttga ttaatggtgt atttactgac attaatccat acattaaaaa tatgatgtac
    31441 gtgttagcag ataatagaca tatctcatta atagctgacg tattcaaggc gttccaaagc
    31501 ttatataacg gacactacaa tcaagatttt gcaacaattg agtcaacata tgaattgagt
    31561 caagaagagt tagataagat tgtcaaacta gtaactcaac aaacgaagtt atctaaagtt
    31621 attgtagata caaaaattaa tccagattta attggtggat ttagagttaa agtcggcaca
    31681 actgtattag atggtagtgt tagaaatgat cttgtccaat tacaaagaaa atttagaaga
    31741 gttaattaat tataaagagg agtgacatag atggccataa aagctgaaga aatcagtgca
    31801 ttacttcgct cacaaattga aaattatgag tcagaaatgt ccgtaactga tgtaggtact
    31861 gtattacaaa ttggtgatgg tattgcatta attcacggat taaatgacgt tatggctggt
    31921 gagctagtag aattccataa cggcgtactt ggtttagccc aaaaccttga agagtcaaac
    31981 gtgggtgtgg ttattttagg accatacaca ggtattactg aaggtgacga agttaaacgt
    32041 actggtcgta tcatggaagt accagtaggt gaagaactaa tcggaagagt tgttaatcca
    32101 ttaggacaac ctattgatgg acaaggaccg attaacacaa ctaaaacacg tccagtagag
    32161 aaaaaagcta ctggtgtaat ggatcgtaaa tcagtagatg agccattaca aacaggtatc
    32221 aaagcaattg atgctttagt accaattggt agaggtcaac gtgagttaat catcggtgac
    32281 cgtcaaacag gtaaaacaac aattgcaatt gacacaattt tgaaccaaaa agatcaaggt
    32341 acgatttgta tctatgttgc tattggtcaa aaagattcaa cagtaagagc aaatgttgaa
    32401 aagttaagac aagcaggcgc tttagactac actattgttg tagcagcatc agcttctgaa
    32461 ccttctccat tattatatat tgcaccatat tcaggtgtaa caatgggtga agaattcatg
    32521 tttaacggta aacatgtttt aatcgtttat gatgatttaa ctaaacaagc agcagcttat
    32581 cgtgagttat cattattatt acgtagacct ccaggtcgtg aagcataccc aggtgacgta
    32641 ttctacttac atagtagatt attagaaaga gcagcaaaat taaacgatga cttaggtggc
    32701 ggttcaatta ctgcattacc aattatcgaa acacaagctg gtgatatttc agcttatgta
    32761 ccaacaaacg ttatttcaat tacagatgga caaatcttct tacaatctga tttattcttc
    32821 tcaggtgtaa gaccagcgat taatgctgga caatctgtat ctcgtgttgg tggatctgca
    32881 caaattaaag caatgaagaa agttgctggt acgttacgtc ttgacttagc gtcatacaga
    32941 gaacttgaat catttgcaca attcggttca gaccttgatg aatttactgc aagtaaatta
    33001 gaacgtggta aacgtactgt tgaagtctta aaacaagatc aaaacaaacc attaccagtc
    33061 gaacaccaag tgttgattat ttatgcatta acaaaaggat atttagatga tattccagtt
    33121 gtagatatca cacgttttga agatgagtta aaccactggg cagaatcaaa tgctactgaa
    33181 ctgttaaatg aaatcagaga aactggtggc ttaccagatg ctgagaagtt tgacacagca
    33241 attaacgaat tcaaaaaaag ctttagcaaa tctgaataat aaacaagttt agtataaggt
    33301 ggtgagatag tggcttctct taaagaaata gatactcgaa taaaatcaac caaaaaaatg
    33361 aagcagatta cgaaagcgat gaacatggta tcaagttcaa aacttcgtag agctgaaaaa
    33421 aatacaaaac aattcacacc atatatggat aaaatgcaag atgcaattac tgcagttgca
    33481 ggggcaagta gcaatacaaa ccatccaatg ctaagacctc gtaaaattac tagaagtgga
    33541 tatttagtta tcacgagtga taaaggttta gcaggtgcat atagtgcaaa cgtgcttaaa
    33601 aaattgatta ctgatattga agcgaaacat caagatagta gcgaatacag tattgtagtt
    33661 ttagggcaac aaggtgttga tttcctaaaa aatagaggtt atgacattga gtattctcaa
    33721 gtagacgtac ctgatcaacc ttctttcaaa tctgttcaag cactagctaa ccatgctata
    33781 gacttataca gtgaagaaga aattgatgaa ttaaatatat actatagtca ttatgtcagc
    33841 gttcttgaaa acaagcctac atctagacaa gtattaccat tatctcaaga ggattctagt
    33901 aaggggcatg gtcatttgtc ttcttatgaa tttgagccag ataaagaatc tatcttaagt
    33961 gtaatcttgc ctcaatatgt tgagagtttg atttacggaa caatattaga cgcaaaagca
    34021 agtgagcatg caacacgtat gactgcgatg aaaaatgcca ctgataatgc aactgaactt
    34081 attgatgact tatcattaga atataacaga gcgagacaag cagaaattac gcaacaaatt
    34141 actgaaattg ttggtggttc cgcagcgctt gaataatatt taaaggagga aaatagcatg
    34201 ggaattggcc gtgtaactca agttatgggt cctgtaattg atgttcgatt tgaacataac
    34261 gaagttccta aaattaataa cgccttggtt attgatgtgc ctaaagaaga aggtacaata
    34321 caactaacat tagaagttgc gctgcaatta ggtgacgacg ttgttcgtac aattgcgatg
    34381 gattcaactg atggtgtcca aagaggcatg gatgtaaaag atacaggcaa agaaattagt
    34441 gtacctgttg gtgacgaaac attaggtcgt gtatttaatg tactaggtga aacaattgac
    34501 cttaaagaag aaattagtga ttctgttcgc cgcgatccta tccatcgtca agcaccagca
    34561 ttcgatgaac tttcaacaga agttcaaatt ttagaaacag gtattaaagt agtagattta
    34621 ctagcacctt atattaaagg tggtaaaatc ggattgttcg gtggtgccgg tgtaggtaaa
    34681 acagtattaa tccaagaatt aattaacaac atcgctcaag agcacggtgg tatttctgta
    34741 ttcgccggtg taggtgaacg tactcgtgaa ggtaacgatt tatacttcga aatgagtgac
    34801 agtggtgtaa ttaagaaaac agccatggta ttcgggcaaa tgaatgagcc acctggtgca
    34861 cgtatgcgtg ttgcattatc tggtttaaca atggctgaat atttccgtga cgaacaaggt
    34921 caagacgtat tattattcat cgataacatt ttcagattta cacaagctgg ttctgaggta
    34981 tctgcattat taggtcgtat gccttctgca gtaggttacc aaccaacact tgctactgaa
    35041 atgggacaat tacaagaacg tattacgtct acaacaaaag gatcagttac ttctattcaa
    35101 gcggtattcg tacctgccga tgactatact gacccagcgc ctgcgacagc gtttgcccat
    35161 ttagatgcaa ctacaaactt agaacgtaaa ttaactgaaa tgggtattta tccagccgtg
    35221 gatccattag cgtctacatc aagagcattg gaaccatcaa ttgtaggtca agaacattat
    35281 gaagtagctc gtgatgtaca atcaacactt caaaaatacc gtgaattaca agatatcatt
    35341 gctatcttag gtatggacga attatctgat gaagataaac aaacagttga acgcgcacgt
    35401 agaattcaat tcttcttatc tcaaaacttc cacgtagcgg aacaatttac tggtcaaaaa
    35461 ggttcttatg tacctgttaa gacaacagtt gcaaacttta aagatatctt agatggtaaa
    35521 tatgaccata ttccagaaga tgctttccgt ttagttggta gcatggatga tgttattgca
    35581 aaagctaaag atatgggtgt tgaagtataa caattaggag gaatggataa tgaatacatt
    35641 aaacctagat attgtcactc ctaatggttc tgtttacaat cgtgataatg ttgaactcgt
    35701 tgttatgcaa acaacagctg gtgagatagg tgtcatgagt ggacatattc caactgtagc
    35761 tgctttaaaa acaggctttg taaaagtgaa atttcacgat ggaactgaat atattgctgt
    35821 aagcgatggc tttgttgaag ttagaaaaga taaagtttca atcattgttc agactgcaga
    35881 aactgcaaga gaaattgatg ttgaaagagc taaattagcc aaagcaagag cagagtctca
    35941 cttggaaaat gatgacgaca atactgatat tcatagagcc gaaagagctt tagagagagc
    36001 aaataaccgt ttgcgtgtgg ctgaattaaa atagtaaata aagggtcgaa gatgtgattt
    36061 catatcttcg accctttttt gaattatatt gatttaaaga tacaaaacat gagagggggg
    36121 aaggaattga taaagaacta ttaaagattt atgatgtagt ggttctttat cattaaacac
    36181 agctaatgtg tatttaaaaa taggaataca tgagtaaaac tcatgtataa gaaatactaa
    36241 tttctaaaga aaaagtattt ctttatgttg gggccccgtc aactactgcc aaatacaaca
    36301 ctatagagtc tagacattga tttatgtccg actcccaaga atagttttac ttttttacaa
    36361 tcactaatag attgctaaaa tcaaaatttc attcaccact atctacagtc gacatttcat
    36421 tttttgaaag tagctacatt ttttcatacc aagatacttt atagttatga tatttatgta
    36481 aaaagaatta tatagtaagt tagcttaaac tttactaaaa acgggtatta aactttgtat
    36541 cattatttaa atttttcatg tacaatgtaa tacagtaatc ttatgaggtg ataaaatgga
    36601 ttatatcgga caatatgcag ttatccattt agtgttacat gttgtatgta tttgtattgc
    36661 ctattgggct ttacaatcaa ttagattaga tcaatttttt aaaaaaggat acgccactca
    36721 attacaagtg tgtatgatat ttgttgctat tttattaggc actgcagtaa gcaattttat
    36781 tgtagatttg ttacaatact cgacgcaggt aaaatattta ataaaataag tctaactcta
    36841 tgatttgtaa tcaaaactag atataattaa ataatgactt aaaataattt taaaataggg
    36901 aaatgtaaag taataggagt tctaagtgga ggatttacga tggataaaat agtaatcaaa
    36961 ggtggaaata aattaacggg tgaagttaaa gtagaaggtg ctaaaaatgc agtattacca
    37021 atattgacag catctttatt agcttctgat aaaccgagca aattagttaa tgttccagct
    37081 ttaagtgatg tagaaacaat aaataatgta ttaacaactt taaatgctga cgttacatac
    37141 aaaaaggacg aaaatgctgt tgtcgttgat gcaacaaaga ctctaaatga agaggcacca
    37201 tatgaatatg ttagtaaaat gcgtgcaagt attttagtta tgggacctct tttagcaaga
    37261 ctaggacatg ctattgttgc attgcctggt ggttgtgcaa ttggaagtag accgattgag
    37321 caacacatta aaggttttga agctttaggc gcagaaattc atcttgaaaa tggtaatatt
    37381 tatgctaatg ctaaagatgg attaaaaggt acatcaattc atttagattt tccaagtgta
    37441 ggagcaacac aaaatattat tatggcagca tcattagcta agggtaagac tttaattgaa
    37501 aatgcagcta aagaacctga aattgtcgat ttagcaaact acattaatga aatgggtggt
    37561 agaattactg gtgctggtac agacacaatt acaatcaatg gtgtagaatc attacatggt
    37621 gtagaacatg ctatcattcc agatagaatt gaagcaggca cattactaat cgctggtgct
    37681 ataacgcgtg gtgatatttt tgtacgtggt gcaatcaaag aacatatggc gagtttagtc
    37741 tataaactag aagaaatggg cgttgaattg gactatcaag aagatggtat tcgtgtacgt
    37801 gctgaagggg aattacaacc tgtagacatc aaaactctac cacatcctgg attcccgact
    37861 gatatgcaat cacaaatgat ggcattgtta ttaacggcaa atggtcataa agtcgtaacc
    37921 gaaacagttt ttgaaaaccg ttttatgcat gttgcagagt tcaaacgtat gaatgctaat
    37981 atcaatgtag aaggtcgtag tgctaaactt gaaggtaaaa gtcaattgca aggtgcacaa
    38041 gttaaagcga ctgatttaag agcagcagcc gccttaattt tagctggatt agttgctgat
    38101 ggtaaaacaa gcgttactga attaacgcac ctagatagag gctatgttga cttacacggt
    38161 aaattgaagc aattaggtgc agacattgaa cgtattaacg attaattcag taaattaata
    38221 taatggagga tttcaaccat ggaaacaatt tttgattata accaaattaa acaaattata
    38281 cctcacagac agccattttt attaattgat aaagtagttg aatatgaaga aggtcaacgt
    38341 tgtgtggcta ttaaacaagt atcaggaaac gaaccattct ttcaagggca ttttcctgag
    38401 tatgcggtaa tgccaggcgt attaattact gaagcgttag ctcaaacagg tgcggtagct
    38461 attttaaata gtgaagaaaa taaaggtaaa atcgctttat ttgctggtat tgataaatgt
    38521 cgttttaaac gtcaagtagt acctggtgat actttaacgt tggaagtaga aatcactaaa
    38581 attaaaggac caatcggtaa aggtaatgct aaagctactg tcgatggtca acttgcttgt
    38641 agttgtgaac ttacatttgc aattcaagat gtaaaataaa acaaaaaaac attcaaagat
    38701 ttaatgtgtt ggcaaaatct ttgaatgttt tttattttac tcttctaatt tttcatcctt
    38761 taactttggt ttagactgca tcattcgatt aaatgatttt tttaattctt caccagataa
    38821 tccatcatca ataagttgtt ctaataaact ttcagcatac tgttgacgta aagtgtaaat
    38881 atgacattca aatacgatag acattgttgt tttaaaattt ttaatacttt tcacagtaga
    38941 ttcaaataaa gctggatcat tggctggacg cgtgatgacg acacgaatat ccaataactg
    39001 ttcattgtct tgatattcct taaatgtttc ataatgctca ttagaaatga caatttcaag
    39061 caaccaaccc gtgccacttt cctctttatt aattattacg ccatcttcta attcatattc
    39121 tataattctg ccgtgttcat tcacaatttg aaaccttact gctttaaaag ttttcatatt
    39181 cccacatccc attagtttaa tatttatgat ttttgaatta tttagaagat ttaaggtcat
    39241 ttcatgacta atttctttaa atataacaaa taacgagatg ctatagctat taattatata
    39301 aaatttgcca ttttgaccat ttttaagcta gtaattaaga tataggcata ggaggtgaat
    39361 cacacactac atgctaaata aaatcgtaat tgtcgagaga ctgacgaaag acgcacaaat
    39421 atttgaaaag gaggatagaa aaattgcaac gttttgtgtt gcaacgcacc gaaattataa
    39481 agatgaaaat ggagaaatcg tctgtgatta cttattctgt aaagcatttg gcaagttagc
    39541 ttctaatata gaaaaatata ctaatcaagg tacattggtt ggtataactg gtcaaatgag
    39601 atcaagaaag tatgataaag acggacaaac acactttgtc actgaattat atgttgaaac
    39661 aataaaattt atgtccccta aatcccaaaa taatgaaatt ctctcagata gtattttaga
    39721 tattgactct caaaatatag ataatcatga cttattagaa atttaaatcc aattatccta
    39781 aacatcctta atatacattt aatccatcct aaatttttta tagatctaat taacttgaaa
    39841 tatactcact taaaaacgac ttacatgact agtactgttg tgtaggtcgt ctttttgtgt
    39901 agtttttcta atagaaaata gacatagatt gcaaaacgat tacataaaaa attgcaaata
    39961 aaaaacattt ttactgtcat gacaattctc gatagaatca tatctgaaat tatgggggat
    40021 ttcgttgtaa tcactgtgta aaaaacaagg ctattttttt attttgttgt aacctgcacg
    40081 gattttatag atgttacagt agtctctgta aaacttttag agaaacaaat tacttgtagg
    40141 aggattttaa gattatgaag aaaacattac tcgcatcatc attagcagta ggtttaggaa
    40201 tcgtagcagg aaatgcaggt cacgaagccc atgcaagtga agcggactta aataaagcat
    40261 ctttagcgca aatggcgcaa tcaaatgatc aaacattaaa tcaaaaacca attgaagctg
    40321 gggcttataa ttatacattt gactatgaag ggtttactta tcactttgaa tcagatggta
    40381 cacactttgc ttggaattac catgcaacag gtactaatgg agcagacatg agtgcacaag
    40441 cacctgcaac taataatgtt gcaccatcag ctgttcaagc taatcaagta caatcacaag
    40501 aagttgaagc accacaaaat gctcaaactc aacaaccaca agcatcaaca tcaaacaatt
    40561 cacaagttac tgcaacacca actgaatcaa aatcatcaga aggttcatca gtaaatgtga
    40621 atgctcatct aaaacaaatt gctcaacgtg aatcaggtgg caatattcat gctgtaaatc
    40681 caacatcagg tgcagctggt aagtatcaat tcttacaatc aacttgggat tcagtagcac
    40741 ctgctaaata taaaggtgta tcaccagcaa atgctcctga aagtgttcaa gatgccgcag
    40801 cagtaaaatt atataacact ggtggcgctg gacattgggt tactgcataa gccatttatg
    40861 catagctaat taatagttat ataagtaact tttagattgg aatatatcgg ggaattctcg
    40921 atatgttccg gtttttttat ttcgcaaata tatgtcatta tacaggctgg gacataaatc
    40981 aatgttctat gctctacgaa gttatattgg cagtagttga ctgaacgaaa atgcgcttgt
    41041 aacaagcttt tttcaattct agtcaacctt gccggggtgg gacgacgaaa taaattttgc
    41101 gaaaatatca tttctgtccc actcccaact gagcaacaat aattgaaaat gttgaaaccg
    41161 tttgctaaga ttaatttgca attgaatact aaggaactac taggggagcc taatgatatg
    41221 gctgagatga attgtttaga cccttatgac ctgatttggt tagtaccaac gtaggaaagt
    41281 agttattgaa gtgatggggc tagtatagca acattgccat caaactactt tcatgttgga
    41341 agtagttttt ttatttaggg aggatttttt acaatggaat tttcacaaaa attgtaccaa
    41401 gctgcaaagc caatcattaa tgatatttat gaggatgact ttattcagaa gatgttatcc
    41461 ggagatatag gagctgacgc attacgccat tacttaaaag ctgatgctgc gtatttaaaa
    41521 gaatttacaa acttatatgc tttgttaatt ccaaaaatga atagcatgaa tgacgtaaaa
    41581 tttttagtag agcaaataga atttatggtt gaaggtgaag tacttgcgca tgatatttta
    41641 gcgcaaattg ttggtgaatc atacgaagaa atcattaaaa caaaagtttg gcctccaagt
    41701 ggcgaccatt acataaaaca tatgtatttc caagcgcata gtcgtgaaaa tgctatttat
    41761 acaattgctg caatggcacc ttgtccatat atttatgcag aattagctaa gcgctctcaa
    41821 agtgatcata agcttaatag agaaaaagat acggcaaaat ggtttgattt ttatagtact
    41881 gaaatggatg acataattaa cgtatttgag gcattaatga ataaattagc cgaatcaatg
    41941 tctgacaagg aattggaaca agtaaagcaa gtatttttag aaagttgcat acatgagcgt
    42001 cgatttttca atatggctat gacactagaa caatgggaat ttggaggaaa agtaaatgat
    42061 taaacctaaa atagcattaa ccattgcagg tactgatcca acaggtggtg ccggcgtaat
    42121 ggctgattta aaatcatttc attcatgtgg tgtatatggt atgggcgtcg ttacaagtat
    42181 tgttgctcaa aatacattgg gcgtacaaca tattcataat ttaaatcatc aatgggtaga
    42241 tgaacaactt gatagtgtct tcaatgatac cttacctcat gctattaaaa cggggatgat
    42301 tgctacagca gatactatgg aaacgattcg tcattattta atgcaacatg aatctattcc
    42361 atatgtaatt gatcctgtta tgttggcgaa aagcggtgat tcattaatgg ataatgacac
    42421 aaagcaaaac ttgcagcata cgttattgcc attagctgac gtagtaacac cgaatttacc
    42481 agaagctgaa gaaataacgg gactaaccat tgatagtgaa gaaaaaatta tgcaggctgg
    42541 ccgcatcttt attaatgaga ttggtagtaa aggtgtcatc attaaaggcg gtcattcaaa
    42601 tgatactgat atagcaaaag attatttatt tactaacgaa ggtgttcaaa catttgaaaa
    42661 tgaacgattt aaaacaaaac atacgcatgg aacagggtgt acattttcag cagttataac
    42721 ggcagaactt gcaaaaggta gacgattatt tgaggctgta cacaaggcta aaaagtttat
    42781 ttcaatgagt atacaatata cgcctgaaat cggccgtggt agaggtccag tgaatcattt
    42841 tgcatattta aagaaagagg gattagacga tgaattatct aaataaaata cgtattgaaa
    42901 acccattaac aatttgttat acaaacgatg tagttaaaaa ttttacagcg aatggtttat
    42961 taagtattgg tgctagccct gcaatgagtg aagctcccga agaagctgaa gaattttaca
    43021 aagttgcaca agggctatta atcaatatcg gtactttaac agcagaaaat gaacaagata
    43081 ttattgcgat tgctcaaacg gcaaatgagg caggcttacc tattgtattt gaccctgtag
    43141 ctgttggtgc ttctacatat cgaaagcaat tttgtaaatt attattgaaa tcagcgaaag
    43201 tatcagtaat taaaggcaat gcatctgaaa tattagcgtt gattgatgat acagcaacta
    43261 tgaaaggtac agatagtgat gctaatcttg atgcggttac aatagcgaaa aaggcttacg
    43321 caacatataa aactgcaata gtaatcacag gtaaagagga cgttattgtt caagataata
    43381 aagccatcgt attagctaat ggatctccat tattagcacg agtaactgga gctggttgtt
    43441 tattaggagg cgttattgct ggatttttat ttagagaaac agaaccagac atagaagcgt
    43501 taattgaagc ggtaagcgta tttaatattg ctgctgaggt agctgctgaa aatgaaaatt
    43561 gtggtggtcc tggtacgttt tcaccattgt tgcttgatac gttatatcat ttaaatgaaa
    43621 caacctatca acaacggatt cgtattcaag aggtggaaaa atatgtttaa ccaatcgtat
    43681 ctaaatgtgt attttatttg tgggacctcc gatgttccga gtcatcgaac tattcatgaa
    43741 gtattagaag cagcattaaa ggcagggatt acacttttcc aatttcgaga aaagggtgaa
    43801 tcagctttaa aaggaaacga taaacttgta ttagctaaag aattacagca tctctgtcat
    43861 caatatgatg tgccttttat cgttaatgat gatgtatcct tggcaaaaga aattaacgcg
    43921 gatggtatac atgtcggtca agatgatgcg aaagtcaaag agatagcaca atatttcact
    43981 gataaaatta ttggacttag tattagtgat ttagatgagt atgcaaaatc tgatttaact
    44041 catgttgatt atataggtgt tggaccaatc tatcctacgc cgtcgaagca tgacgcgcat
    44101 ataccagtag gtccagaaat gattgcaacg tttaaagaaa tgaatccgca actaccgatt
    44161 gtagctattg gcggtatcaa tactaataat gtggcgccga ttgtagaagc aggtgccaat
    44221 ggaatttcag tcatttcagc catttctaaa agtgaaaata ttgaaaatac tgttaatcga
    44281 ttcaaagatt tttttaataa ttagttaaat ttttccactt attgtttcat acgtactcat
    44341 taatgtgata gaataaatcc tatgtgaata taatgataga ggtggaaaaa tgaagaaaaa
    44401 agcgttacta ccattatttt taggtattat ggtctttttg gctggttgtg actattctaa
    44461 acctgaaaaa cgtagtgggt ttttctacaa tacattcgta gatccaatga aaaatgtatt
    44521 ggattggttg ggaaataact tattaaacga caattatggt ttagctatta ttatccttgt
    44581 attggtaatt cgtattattt tattaccatt catgttgtca aactataaaa atagtcatat
    44641 gatgcgtcaa aaaatgaaag ttgcaaagcc agaagttgaa aaaattcaag aaaaagtgaa
    44701 acgtgcgcgt acacaagaag aaaaaatggc tgcaaaccaa gaattaatgc aagtatataa
    44761 aaagtatgac atgaacccga ttaagagtat gttgggttgt ttaccaatgc taatccaatt
    44821 gccaatcatc atgggattat actttgtact taaagaccaa cttgtagatg gtttgtttaa
    44881 atatccacac ttcttatggt tcgatttagg acgtcctgat atttggatta caattattgc
    44941 cggtgtttta tactttatcc aagcatatgt atcaagtaaa acgatgccag acgaacaacg
    45001 tcaaatgggt tacatgatga tggtcatttc accaattatg attatctgga tttcattaag
    45061 ctcagcatca gcacttggtt tgtactggtc agtcagtgcg gcgttccttg tagttcaaac
    45121 acactttgcg aacatttatt atgaaaaagt cgctaaaaaa gaagtacaac ctttcattga
    45181 agcgtatgaa agagagcaca acggcggcag caataaaaaa ggcaaaaaca cacaagtagt
    45241 gtctaaaaag aaaaagaaat aaccttagca agtccaaata atgtgcaccc taaaagttga
    45301 atataatttt aactttatag ggcgcacatt atttttcgct tcaaaagcga aacttagtgt
    45361 cacattatac aatatgtgat aaatagaaac agcagtaaga tgatttcaaa tgtgaaagtc
    45421 atcctactgc tgtttttatt tgaaataata ttaacgagtt ttcagtatgt aaaaagtggt
    45481 taataacaga gttgtcattc ttcatcgcaa tgtcatcttg acacaatgat tcatcagtgt
    45541 agcagtatat gtcgcaaatt tccacagcaa ttcacttata catgtaacag tgtgaaataa
    45601 taacgtcaca acggcataat acacattgca acgtcccaac aacatatcat agaatcgtaa
    45661 cggcatggca tcgacgccaa accggtctat aacaccataa caccgcaatg ccacatcata
    45721 gcactacatc aagtcgcatc gtagcaccag cacatcaaag taacatggca tcaacaccaa
    45781 gcttccacaa caacacatca aagtaacatg gcatgacacc aagcttccac aacaacacgc
    45841 cattacatcg catcatcata ctacaacttc aatgtctagt cgtgataatt ccattccgta
    45901 aaaaactgtt tcaagtacat catcataaag tcgtgacgtt ctttagcaat catcttcgcc
    45961 gtatctgtat gcattaaaga ttctaactta agtaattttt caaagaaatg tttaattgca
    46021 gatggtggca actgttcaac taaatcatca ttaatcttat ctagtgacat atgttctgtc
    46081 cacattggtt caccaaagtg tcctgcaaat tgaaatgttc gtgcaacacc tatagcgcct
    46141 atagcatcaa gacgatctgc atccctgaca atttgacctt ctaaagataa agtgacatga
    46201 tcatttttgc cattgcgata gctcatatta ttaataataa ataaaatgtg ctcttgatct
    46261 tcggttgata gtgataaaga agataaaaat gacttcaatt caacatatag tttgttagca
    46321 tctacaactt tttcgtcaac ggtatcatga agcaaacatg cgagttcaat gactaaagta
    46381 tcattaacac cttcattttc agcgattgat ttagctaaag cagtgacacg atatacatgc
    46441 gcaatatcat ggcctgtata atcattttga tgaatagatt ccatatattt tttggcagca
    46501 ctaatgacgt ctgattgttg cattaaagtt acactcctca tatttctatt ttataagata
    46561 ggtgacaata attgtgaaat accttcttta aatttaatcc aaagacttcg cttagcataa
    46621 cgtgcttttg ttaattcaga agatactgct aaatcatcta taaaagcttg ttttaatttt
    46681 ttggcaattt gttggtcata aataaaggcg ttgacttcga aattcaatgt gaaactgcga
    46741 tggtccatat tagctgttcc aacacttgca atttcatcat ctataacaag tgtttttgag
    46801 tgtaaaaagc cattgtcgta gtgaaatact ttaacaccgg catctaataa ggatgctgca
    46861 ttttttaaag tagcccaaaa aacaaacgga tggtcaggtt tattaggaat catgatattg
    46921 acatcaacac cacctaatgc cgcaatttta atagaatcta aaaaggcttg atcaggtata
    46981 aaatagggag attgaatata aatcgatttt ttagcagatg aaatcatttt caaataaccg
    47041 tatttaatct gttcccattc ttcgtcagga ccactagaag ctatttgaac gccaattgtt
    47101 ccaccagaat ttacatctgg gaaataacga tcatcatagg agatgtggtc acgtgtggct
    47161 tgtgaattcc aatctagaat aaatcgtaat tgcaatgcat tcactgcgtc cccgacaatt
    47221 cgtaaatgcg tatctcgcca atagccgaat ttttttgatt tacctaagta ctcatcacca
    47281 acattaaaac caccaacata tccaatttgc ccatctatta caacaatttt tcgatggttt
    47341 cgattgttca tacgcaagtt aattaaaggt aattttgatg ggaaaaatgc ttcagcatgt
    47401 ccacctttat tgcgaaacgg gcgtaagcct tttttacgca gtccacgaga acccatgtca
    47461 tcataaagaa ttttaacttc tacaccttgt tccgcttttt taccaagttc atttaaaatg
    47521 gtacgaccta attcatcatt ttgaataata tagtactgaa aatgaatata atcagtagca
    47581 ttacggatgt cttgtattag gtcatcaaat ttttcttggc cgtctgtgta tatttttaaa
    47641 tcattgtctg ttgttaaaaa tgctgcgtta ttatataaca acatttgaat catttcttta
    47701 aattttacaa tttgataatt ggaatttgaa aagttttcat tttttaaagc agctaattgc
    47761 tcatcaacga ttaactctaa tcctttttta tcttccttat caattttgaa aatttggtca
    47821 cgttgaattt gtcgtcctaa tagtaagtat aaaatgaagc cgaataaagg caagaaaact
    47881 aagactagta accaagccca gatagaattg gcagaacgtc tttccatgaa aataatggta
    47941 aaggcgaata ataagtttaa aataaatgca ccaataaata ttgaatttaa aataatatta
    48001 gaatgtttga gtgcaatgga taataactct atcatttgaa acctcccatc gaaaatctta
    48061 aacgagtagt atatcttatt aattgtatca tgaagcaatt taaatatcat gacaatttag
    48121 tttgaaaaac acactttgtg ttgacgttgt gttttatggg atatagtata gtaaagaatg
    48181 taaatgaagg agtgaatgct atgactgaac aagataatgc acatcattct gaacaaataa
    48241 aaacgaatct taaatcacgt ttaaatcgaa ttgaaggaca agtgagagcg attaatcgca
    48301 tgattgaaga agatgtctat tgtgatgatg tccttacgca aataagagcg acacgttcgg
    48361 cgttaaacag tgttgcgata aagttattag aacaacatat gaaaagttgt attatgaata
    48421 aagttaatca aggtgctcag gaagaggcga tggaagagtt attagtgact tttcaaaaat
    48481 tgattaaaga ctaaaggagt ttaagatgat acatcaaaat acgatttaca cagcgggaat
    48541 tgaaacagaa gaacaagtaa gtcaattgac agaacgcatt tcaaatatga taggtgttca
    48601 tcaagtgaat attaatataa tagatggtca agtaactgta tcgtatgaga caccagcaaa
    48661 tttgaatagt attgaaaaag aaatctatga tgaaggatac aaaattgtaa tttagggtat
    48721 aatgtagagt gcgctataga ttttaatttt gaaaataaat taaaaatttt gtaaatgatg
    48781 tagtaaaggt atgtcgaata gggtaagata atattgaaag cgatccaaac gaggtgataa
    48841 taatgaaacg tcctgaaaag attcaaaatg tagtcaaact attgtcatca ttaggtgtga
    48901 atattaaaaa aactaaatct cgtttagaca ttattaatac tttgccagca tctaataaag
    48961 taagtcacga attaaaataa ttgactgtga ttgtgaatca cacaccgtta aagctatcgt
    49021 tggactattc gatgatagct tttttgactg gatttactaa aatgaaagca agtagtatat
    49081 aactaaatcc ctctttagag tatacatttt acatgtgtac ctcaaagagg gattgttatt
    49141 atcgagtaat atctatatat gacattttaa atcgacgata taaatagact atataatgtc
    49201 atcttgaaaa gttatgatat ttgaaactca aaatagttta aattaattac tttttggatg
    49261 gtataaatcg acatatcgtt ttggttcatg ataataaatt gataagacta tacctattcc
    49321 agtcatcata ctccatagcg cactaccacc ataactaata aatggtaatg gaataccagt
    49381 gattggtaac aactgaattg tcataccaat attttgtaaa atatggaaca caagtaaagt
    49441 aacgaaacca acgataaaga ttttgttaaa ttgatcttca attttcgcag ctaatcttat
    49501 tagatggaag attaaaaata aaaatattaa gatcaatatg acagaaccga taaaacctag
    49561 ttcctctcca atcactgaaa agataaagtc agtatgattt tcaggtatat aaacttcacc
    49621 gtgattgtat cctttaccta gtaactgtcc agaaccgata gctttaagtg attcagttaa
    49681 atgatagcca tcgccactac tatatgtata ggggtcaagc catgaattga ttcgtcccat
    49741 ttgatacagt tggacaccta ataaattttc aattaatgcg ggtgcatata gaatacctaa
    49801 aatgactgtc attgcaccaa caatacctgt aataaagata ggtgctaaga tacgccatgt
    49861 tataccactt actaacatca cacctgcaat aattgcagct aatactaatg tagttcctag
    49921 gtcattttgc agtaatatta aaatacttgg tactaacgag acaccaataa ttttgaaaaa
    49981 taataacaaa tcactttgga atgatttatt gaatgtgaat tgattatgtc tagaaacgac
    50041 acgcgctaat gctaaaatta aaataatttt catgaattca gatggctgaa tactgatagg
    50101 gccaaacgtg taccaacttt tggcaccatt gataataggt gtaataggtg actcaggaat
    50161 aacgagcaag cctattaata atagacagat taagaaatac aataaatatg tataatgttt
    50221 aatcttttta ggtgaaataa acatgatgat acctgcaaaa attgcaccta aaatgtaata
    50281 aaaaatttgt ctgataccga aattagcact gtattgacca ccgcccattg ccgagttaat
    50341 aagcagaaca ctgaaaattg ctaaaacagc tatagtggct actaataccc agtctacttt
    50401 gcgaagccaa tgcttatccg gctgttgacg agatgaataa ttcattgcaa actcctttta
    50461 tactcactaa tgtttatatc aattttacat gactttttaa aaattagcta gaatatcaca
    50521 gtgatatcag ctatagattt caatttgaat taggaataaa atagagggga atattgttct
    50581 gattataaat gaatcaacat agatacagac acataacgcc tcgtttttaa aatgcaaaat
    50641 agcattaaaa tgtgatacta ttaagattca aagatgcgaa taaatcaatt aacaatagga
    50701 ctaaatcaat attaatttat attaaggtag caaaccctga tatatcattg gaggaaaacg
    50761 aaatgacaaa agaaaatatt tgtatcgttt ttggagggaa aagtgcagaa cacgaagtat
    50821 cgattctgac agcacaaaat gtattaaatg caatagataa agacaaatat catgttgata
    50881 tcatttatat taccaatgat ggtgattgga gaaagcaaaa taatattaca gctgaaatta
    50941 tatctactga tgagcttcat ttagaaaatg gagaggcgct tgagatttca cagctattga
    51001 aagaaagtag ttcaggacaa ccatacgatg cagtattccc attattacat ggtcctaatg
    51061 gtgaagatgg aacgattcaa ggactttttg aagttttgga tgtaccatat gtaggaaatg
    51121 gtgtattgtc agctgcaagt tctatggaca aacttgtaat gaaacaatta tttgaacatc
    51181 gagggttacc acagttacct tatattagtt tcttacgttc tgaatatgaa aaatatgaac
    51241 ataacatttt aaaattagta aatgataaat taaattaccc agtctttgtt aaacctgcta
    51301 acttagggtc aagtgtaggt atcagtaaat gtaataatga agcggaactt aaagaaggta
    51361 ttaaagaagc attccaattt gaccgtaagc ttgttataga acaaggcgtt aacgcacgtg
    51421 aaattgaagt agcagtttta ggaaatgact atcctgaagc gacatggcca ggtgaagtcg
    51481 taaaagatgt cgcgttttac gattacaaat caaaatataa agatggtaag gttcaattac
    51541 aaattccagc tgacttagac gaagatgttc aattaacgct tagaaatatg gcattagagg
    51601 cattcaaagc gacagattgt tctggtttag tccgtgcaga tttctttgta acagaagaca
    51661 accaaatata tattaatgaa acaaatgcaa tgcctggatt tacggctttc agtatgtatc
    51721 caaagttatg ggaaaatatg ggcttatctt atccagaact gattacaaaa cttatcgagc
    51781 ttgctaaaga acgtcaccag gataaacaga aaaataaata caaaattgac taactgaggt
    51841 tgttattatg attaatgtta cattaaagca aattcaatca tggattcctt gtgaaattga
    51901 agatcaattt ttaaatcaag tgataaatgg agtcacaatt gattcacgag caatttctaa
    51961 aaatatgtta tttataccat ttaaaggtga aaatgttgac ggtcatcgct ttgtctctaa
    52021 agcattacaa gatggtgctg gagctgcttt ttatcaaaga gggacaccta tagatgaaaa
    52081 tgtaagcggg cctattatat gggttgaaga cacattaacg gcattacaac aattggcaca
    52141 agcttacttg agacatgtaa accctaaagt aattgccgtc acagggtcta atggtaaaac
    52201 aacgactaaa gatatgattg aaagtgtatt gcataccgaa tttaaagtta agaaaacgca
    52261 aggtaattac aataatgaaa ttggtttacc tttaactatt ttggaattag ataatgatac
    52321 tgaaatatca atattggaga tggggatgtc aggtttccat gaaattgaat ttctgtcaaa
    52381 cctcgctcaa ccagatattg cagttataac taatattggt gagtcacata tgcaagattt
    52441 aggttcgcgc gaggggattg ctaaagctaa atctgaaatt acaataggtt taaaagataa
    52501 tggtacgttt atatatgatg gcgatgagcc attattgaaa ccgcatgtta aagaagttga
    52561 aaatgcaaaa tgtattagta tcggtgttgc tactgataat gcattagttt gttctgttga
    52621 tgatagagat actacaggta tttcatttac gattaataat aaagaacatt atgatctacc
    52681 aatattagga aagcataata tgaaaaatgc gacgattgcc attgcggttg gtcatgaatt
    52741 aggtttgaca tataacacaa tctatcaaaa tttaaaaaat gtcagcttaa ctggtatgcg
    52801 tatggagcaa catacattag aaaatgatat tactgtgata aatgatgcct ataatgcaag
    52861 tcctacaagt atgagagcag ctattgatac actgagtact ttgacagggc gtcgcattct
    52921 aattttagga gatgttttag aattaggtga aaatagcaaa gaaatgcata tcggtgtagg
    52981 taattattta gaagaaaagc atatagatgt gttgtatacg tttggtaatg aagcgaagta
    53041 tatttatgat tcgggccagc aacatgtcga aaaagcacaa cacttcaatt ctaaagacga
    53101 tatgatagaa gttttaacaa gcgatttaaa agcgcatgac cgtgtattag ttaaaggatc
    53161 acgtggtatg aaattagaag aagtggtaaa tgctttaatt tcatagagat tagtcgaggg
    53221 accttttact tataaaaatg atttgaatta atactaaaag attacaaaga agaggtggtt
    53281 ttgtgtgtaa atacaaaatt gcctttttct ttttatgtta aatctataaa tttgaaacta
    53341 aatcaaggtt aattctatgt acacacttta tataggaagt agtttgaatg tttatataat
    53401 gttttacaaa aagatgtagt attataatgt ctaatttcac atgtgtttca gtaaaatttg
    53461 ttgtggaatg tta
//
LOCUS       DBNENW010000003        42104 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNENW010000003 DBNENW010000000
VERSION     DBNENW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.003"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..821)
                     /gene="fakB1"
                     /locus_tag="AC1LW8_000131"
     CDS             complement(<1..821)
                     /gene="fakB1"
                     /locus_tag="AC1LW8_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HIP4772001.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSG"
     gene            965..1606
                     /locus_tag="AC1LW8_000132"
     CDS             965..1606
                     /locus_tag="AC1LW8_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIP4772002.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(1778..2833)
                     /locus_tag="AC1LW8_000133"
     CDS             complement(1778..2833)
                     /locus_tag="AC1LW8_000133"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP4772003.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            3151..4221
                     /gene="gdpS"
                     /locus_tag="AC1LW8_000134"
     CDS             3151..4221
                     /gene="gdpS"
                     /locus_tag="AC1LW8_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein GdpS"
                     /protein_id="HIP4772004.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            4417..5178
                     /locus_tag="AC1LW8_000135"
     CDS             4417..5178
                     /locus_tag="AC1LW8_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP4772005.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            5196..5690
                     /locus_tag="AC1LW8_000136"
     CDS             5196..5690
                     /locus_tag="AC1LW8_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP4772006.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            5704..6930
                     /gene="pepT"
                     /locus_tag="AC1LW8_000137"
     CDS             5704..6930
                     /gene="pepT"
                     /locus_tag="AC1LW8_000137"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIP4772007.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(7112..8236)
                     /locus_tag="AC1LW8_000138"
     CDS             complement(7112..8236)
                     /locus_tag="AC1LW8_000138"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIP4772008.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(8661..8981)
                     /gene="ytxJ"
                     /locus_tag="AC1LW8_000139"
     CDS             complement(8661..8981)
                     /gene="ytxJ"
                     /locus_tag="AC1LW8_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HIP4772009.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(9135..10013)
                     /locus_tag="AC1LW8_000140"
     CDS             complement(9135..10013)
                     /locus_tag="AC1LW8_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HIP4772010.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            10133..10651
                     /locus_tag="AC1LW8_000141"
     CDS             10133..10651
                     /locus_tag="AC1LW8_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HIP4772011.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFHTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            10778..11701
                     /gene="murB"
                     /locus_tag="AC1LW8_000142"
     CDS             10778..11701
                     /gene="murB"
                     /locus_tag="AC1LW8_000142"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIP4772012.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            11719..12033
                     /locus_tag="AC1LW8_000143"
     CDS             11719..12033
                     /locus_tag="AC1LW8_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HIP4772013.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(12350..13378)
                     /locus_tag="AC1LW8_000144"
     CDS             complement(12350..13378)
                     /locus_tag="AC1LW8_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrated catecholamine ABC transporter
                     substrate-binding lipoprotein SstD"
                     /protein_id="HIP4772014.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(13497..14258)
                     /locus_tag="AC1LW8_000145"
     CDS             complement(13497..14258)
                     /locus_tag="AC1LW8_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772015.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(14255..15211)
                     /locus_tag="AC1LW8_000146"
     CDS             complement(14255..15211)
                     /locus_tag="AC1LW8_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIP4772016.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVIFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(15198..16169)
                     /locus_tag="AC1LW8_000147"
     CDS             complement(15198..16169)
                     /locus_tag="AC1LW8_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772017.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(16208..16363)
                     /locus_tag="AC1LW8_000148"
     CDS             complement(16208..16363)
                     /locus_tag="AC1LW8_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772018.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(16877..17848)
                     /gene="nrdF"
                     /locus_tag="AC1LW8_000149"
     CDS             complement(16877..17848)
                     /gene="nrdF"
                     /locus_tag="AC1LW8_000149"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HIP4772019.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(17966..20071)
                     /gene="nrdE"
                     /locus_tag="AC1LW8_000150"
     CDS             complement(17966..20071)
                     /gene="nrdE"
                     /locus_tag="AC1LW8_000150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HIP4772020.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(20034..20432)
                     /gene="nrdI"
                     /locus_tag="AC1LW8_000151"
     CDS             complement(20034..20432)
                     /gene="nrdI"
                     /locus_tag="AC1LW8_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIP4772021.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            21233..22099
                     /locus_tag="AC1LW8_000152"
     CDS             21233..22099
                     /locus_tag="AC1LW8_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIP4772022.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            22119..22619
                     /gene="queF"
                     /locus_tag="AC1LW8_000153"
     CDS             22119..22619
                     /gene="queF"
                     /locus_tag="AC1LW8_000153"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIP4772023.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            22959..24464
                     /locus_tag="AC1LW8_000154"
     CDS             22959..24464
                     /locus_tag="AC1LW8_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIP4772024.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(24542..24643)
                     /locus_tag="AC1LW8_000155"
     CDS             complement(24542..24643)
                     /locus_tag="AC1LW8_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772025.1"
                     /translation="MVITDIDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            24733..25650
                     /locus_tag="AC1LW8_000156"
     CDS             24733..25650
                     /locus_tag="AC1LW8_000156"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIP4772026.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGKYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(26275..26817)
                     /locus_tag="AC1LW8_000157"
     CDS             complement(26275..26817)
                     /locus_tag="AC1LW8_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5' nucleotidase, NT5C type"
                     /protein_id="HIP4772027.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(27112..28170)
                     /gene="hisC"
                     /locus_tag="AC1LW8_000158"
     CDS             complement(27112..28170)
                     /gene="hisC"
                     /locus_tag="AC1LW8_000158"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIP4772028.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTEEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(28410..29924)
                     /locus_tag="AC1LW8_000159"
     CDS             complement(28410..29924)
                     /locus_tag="AC1LW8_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIP4772029.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(29917..30894)
                     /locus_tag="AC1LW8_000160"
     CDS             complement(29917..30894)
                     /locus_tag="AC1LW8_000160"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772030.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDVAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(31116..32897)
                     /gene="recQ"
                     /locus_tag="AC1LW8_000161"
     CDS             complement(31116..32897)
                     /gene="recQ"
                     /locus_tag="AC1LW8_000161"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIP4772031.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(32909..34792)
                     /locus_tag="AC1LW8_000162"
     CDS             complement(32909..34792)
                     /locus_tag="AC1LW8_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP4772032.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVSLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(35063..37003)
                     /gene="ltaS"
                     /locus_tag="AC1LW8_000163"
     CDS             complement(35063..37003)
                     /gene="ltaS"
                     /locus_tag="AC1LW8_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HIP4772033.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(37477..38481)
                     /locus_tag="AC1LW8_000164"
     CDS             complement(37477..38481)
                     /locus_tag="AC1LW8_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HIP4772034.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(38465..39175)
                     /locus_tag="AC1LW8_000165"
     CDS             complement(38465..39175)
                     /locus_tag="AC1LW8_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HIP4772035.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(39263..39469)
                     /locus_tag="AC1LW8_000166"
     CDS             complement(39263..39469)
                     /locus_tag="AC1LW8_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772036.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(39535..40143)
                     /locus_tag="AC1LW8_000167"
     CDS             complement(39535..40143)
                     /locus_tag="AC1LW8_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIP4772037.1"
                     /translation="MQLFETMKIENGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESEFETIVSPLPEKSSFTAKFQVLPKVVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(40143..41294)
                     /locus_tag="AC1LW8_000168"
     CDS             complement(40143..41294)
                     /locus_tag="AC1LW8_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIP4772038.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(41278..41871)
                     /locus_tag="AC1LW8_000169"
     CDS             complement(41278..41871)
                     /locus_tag="AC1LW8_000169"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIP4772039.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT        13657 a         7352 c         6038 g        15057 t
ORIGIN      
        1 ccagaaccta aatgtgctgc aacaactgga ccaaactcag agtatgctac ttgataagct
       61 gaaggacaat catcttgtaa ctttttgtat aacgcttgac catcttcgaa atgatctcca
      121 tttatgacaa ataaagttac ttcttcaaag tcttttacaa tatctaatac tttcttttct
      181 aatgtttgaa tggcacgctt tttagtacga actttttctt ctggtataat cttgccatct
      241 tcaaacttaa gaactggctt cattttcaat aatgttccaa cccatgcttg agcaccagta
      301 attcgaccac ttttttgtaa attctttaag tcatcaacaa ttaaatatgc acctgtgtgt
      361 tcacgcatat tagttaaatc atcaataatt tgctgtggtt cgtatccttc ttcaaccatt
      421 tcaatagcac gtaatacata acatccttca atcattgctg caagcttact atcaaatgca
      481 tgtacattaa caccttcaac catttcccct gcttgataac tagattgata acttccacta
      541 atcccactgg ataagcaaat gacaatgata tctgtataac cctgatctct tagcatttca
      601 tatttagtaa tccattcgcc aattgctggt tggcttgttg ttggaatcgt ttgagacgat
      661 gccattttat tataaaattc ttcaattgct atttcattac tttctgtaaa gttcttccca
      721 tcatcaaaag tcacacttaa tggcgctatt tgaatattat atttatcgat taagtcctgc
      781 gacagataac ttgtagaatc ggtcatcaca gcaattttca tagtcgtcct cctaaaatat
      841 actcatagca atgatacaat atttcagtta aaaagaaaat ggcaatcaaa cgatttatag
      901 tcatgtcatt ttcagtgtat aatgaagatt aaataatctt aagcttatga attaaggagt
      961 tattatgaca caaaatatca ttactgttaa aaaagaacac attattgaaa atgtgattag
     1021 taaatcaaga ttcattgctc atatcaaacc tgttcaaaat gaagatgaag ctaaagcatt
     1081 tattgctgca ataaaaaaag aacataaaga tgccactcat aattgttctg catatactat
     1141 tggacctgaa atgaatattc aaaaagcgaa tgatgacggt gaaccgacag gaactgccgg
     1201 tgtcccaatg ttagacatat taaaaaagct cgatgttcat aatgcgtgtg tcgtagtaac
     1261 aagatatttt ggtggtatta aattaggtgg tggcggttta attcgcgcct atagcggtgc
     1321 tgttagagac gtcatttatg atgtcggacg tgtcgaacta cgagaagcag taccatgtat
     1381 agtaacactg aattatgatc aaactggtaa atttgaatat gaattagctt caactcattt
     1441 tatgttaaga aaccaaactt acactgataa ggtaagctac tatatcgatg tcgttaaaag
     1501 tgactatgaa gcgtttatta actttttaaa tcaaatgact gcaggaaatt tcgatttgac
     1561 tgaagaaact ccaaagcagt tacctttcga tataccaact gaataaaaat aaaaagcact
     1621 atcataagac ggtaaatatc aattgcgtat tattaaacga caattgaagt acacgtttat
     1681 ggcagtgctt ttttatattt gaaatttaat acataaaaaa ttgcaaagag taaagccata
     1741 taatacaaat tatcccatac agctatgctt tcattcccta ttcctctttg tgtgacgatt
     1801 tccgactaat taaatttaat attggtcggt agttgttatc tattaatcct gtaaattcaa
     1861 caattaattc aatactaaat atgattaata caaacattaa tacaacacct aatggtggcg
     1921 atacatacaa aataatgctc gaaagactaa ataaaataga gattgaatag attaataata
     1981 ctgtttgtct atgtgtgtag cctaaagcta atagtttatg atgcaaatgc gatttatcag
     2041 cttgcattat atgctgccct tttttcacac gtcgaatcat tgcgaacaaa gtatcaatga
     2101 atggaactgc taagataaca attgggaaga acaatgcaat aattgtaata tttttgaatc
     2161 cgagtaaaga aaggaatccg atgataaatc caatcattaa agccccacta tcacctaaaa
     2221 atattttggc aggatggaaa ttgtaaaata aaaacccaat taaagagcct aataaaacac
     2281 aacaaatcat cgttatgaaa atattagctt gtaaaattgc aatgaatcct attgtaatga
     2341 gtccgattgc agaaacaccc gacgccaaac catcgagtcc atcaattaag ttaattgcat
     2401 ttgtaatagc aacaatccaa atcacagtaa ttggaatact aagaaatcca aaatgaatcg
     2461 ttgtacccat tggcaacgaa ataaaatcaa tagtcacacc ataaaaagca actactaagg
     2521 cagcggcaat ttgaccagcc aattttatat acggtttcaa atcgtagata tcatctacaa
     2581 gcccaagtac gtacataata atcgcaccaa taataagtgg tttgatttct gtttcaatag
     2641 gatgaccaat ccaaatacct attaaaaatg aaaagagaat cactgtacca cccataactg
     2701 aaataggttt agtgtgtact tttctaaaat ttggtttatc aactaaattt aatcttttcg
     2761 atattgcaat aacaattggt gttatcgtca aactgacaat cattgttact gcaactagta
     2821 ataatgtaac catttattca ccttcatcga tattaattgt ttttaacgtt atatttattt
     2881 aatattgtct ttatagaatg atatatctat tttctacgta tatcttatag ctttattata
     2941 aattaattcg ctcaatcaat gatatcatat ttacaactat acgattcatt tcttatatat
     3001 cgcctattat tcattttttc atcatatcat ttaaggcaac attttaacat caaagtctat
     3061 atcatacatt aattttatat gtaaactaat gacaaattgt aagttttcac ttacaatagc
     3121 tagtggtcaa ttaaaggagt cgattcagac atgttcgaag catttatata caatatatct
     3181 gttatagtcg ctggaatcta tttattccat cgcttacagt attcagaaaa caaacgtatg
     3241 gttttttcta aggcttatgt cactgtttta atgacaatcg tatcattatt attatctgtc
     3301 taccctatcc cttatcgtga agattattta attcacttaa cattcgttcc tttattgttt
     3361 ttaggtagat ttacaaatat ggtctataca ttatctgcca cagtaattgt agcaattgtt
     3421 gaaattgtag tatttaacaa ctcgattatg tacggtgtta cgttgattgt tattgctgca
     3481 gtaacaagtg ctattggacc atttttaaaa caaaatgatg tgctatcatt attaatactt
     3541 aatgttgtta caataatcat tttatttggt gtcgcgttag tgagtcctat ttacacacta
     3601 tcggaagtta tcattttaat accaatttca ttaattatca ctttagcttc agcgataaca
     3661 tttgtcgata tttggcattt cttctcactc gtaaatcgtt atgaaaacga agataaatat
     3721 gattacttaa caggcttagg taatgtaaaa gaatttgata gacatttaaa tgaaatttca
     3781 cgaaaagctg aaaaagaaca tcaaagtatc gctttattat taatcgatat cgatggattt
     3841 aaagatgtca atgataccta ctcacacaaa tcaggtgatg ctgtattaaa acaaatgtct
     3901 caattactta aaaactatgt gccgaatcaa tttaaaattt ttagaaatgg tggcgaagag
     3961 ttctcagttg tcattcacaa ttattcactt gatcaaagtg ttaaattggc tgaaaatatt
     4021 cgttcgggtg ttgaaaaatc atcattccat ttaccaaaca aagaagttat taaattgtca
     4081 gtgtctatcg gtgttggata tttaacagat gacgatccaa aatcacagcg taaagtgttt
     4141 aaagatgcag acgatatggt gcatgtagcc aaaaatcaag ggcgaaacaa agtaatgttt
     4201 aaccctatta tcaatttata acaacgacaa aactgttaat cctttgctat tattaaatca
     4261 tagtataata gcaaagatta acagtttttc ttatttaaat tcgaaccata caagttcatc
     4321 atactaacac aaagtctcac tattgaaagt tgcaacaaca agttgtaaac ttcaattgtt
     4381 caaataaata acacatatca ttggaggtga attgatatgc cagattcaat cacaattata
     4441 gatgaaaaca aagtgattga tgttgtatta attgcaggta gaattttact tgaatcaggt
     4501 gctgaaacat atcgagttga agatacaatg aaccgtatcg cacatagtta tggtcttcat
     4561 aatacatata gttttgtcag ttcaactgca attatttttt cattaaacga tcgaacaagt
     4621 acaagattaa ttcgtgtaca agagcgtaca acagatttag agaaaatcgc tttaacgaat
     4681 agtctttcac gtaaaatatc aaataaagaa ctcacaattg atgaagctaa atctgaattt
     4741 attcatttac agcatgcatc attacaatat tcttttttaa cgaatttctt tgcagctgcc
     4801 attgcatgtg gcttcttcct atttatgttt ggtggtgtcg catcagattg ttggattgca
     4861 gtcattgctg gcggatccgc atttttaaca ttcagctttg tgcaacgcta tatacaaatt
     4921 aaatttttct cagagtttgt tgcagccgct gtggtcatat ccattgcagc cacatttact
     4981 aaattaggca ttgcaaccaa tcaagatatc attactatag ccagtgtcat gccacttgtc
     5041 cctggtatat taattaccaa tgccattcga gatttattag cgggtgaatt acttgcaggt
     5101 atgtctcgtg gtgttgaagc tgcattaaca gcattcgcaa tcggtgctgg tgtcgcaatc
     5161 gttttattaa tcatttaagg aaggactgtt aaagcatgct attttattta tttcatttta
     5221 caatcagctt tatatcaaca gtacttttct ctatcatttt caatgcaccc aaacgcctct
     5281 tagtagcatg tggatttgtg ggtgccattg catggacgat ttaccaatta acggtagatt
     5341 tagagtttgg aaaagttggc gcttcatttt tgggaagctt aattttaggc ttaatgagtc
     5401 atactatgag ccgcagatat aaacgaccgg taattatatt catagtgcca ggcattatac
     5461 cattagtacc tggtggtgca gcttatcaag cgactcgttt tttagtatca aatgattata
     5521 caagtgctgt aaatacattt ttagaagtta cactgatttc aggtgcgatt gctttcggta
     5581 tattagtttc tgaaattcta tattacctat acacacgtat caaacaactg tatggtaaaa
     5641 tcaaaggtaa gacatataaa aaatcttaca acatgaataa tagagtttaa ggggtcgaat
     5701 ataatgaaga accaattgat agatagatta acaagatata cgacaattga tacgcaatct
     5761 gatccaaaat ctacaacaac accttcaact gaaaagcaat gggatttgtt acatttatta
     5821 gaaaaagaat tacaacaatt aggattgcca actgatttag atgaaaatgg ctacttattt
     5881 gctacattag aaagtaacat cgatgtcgat gtcccaacag ttggattttt ggcgcatgtt
     5941 gatacatcac ctgatttcaa tgcttcaaat gttaaaccgc aaattattga aaactatgat
     6001 ggtaagccat acaaactagg caatactaaa cgtgtcttag atcctaaagt atttccagaa
     6061 cttaacagtt tagttggtca tacattaatg gtaactgatg gtacatcgtt actaggtgca
     6121 gacgataaag cgggtattgt ggaaattatg gaagctattt gttatttaca agaacatcca
     6181 gaaattaaac atggtaccat tcgcattgga tttacaccag acgaagaaat cggtcgtggt
     6241 ccacataaat ttgatgttga ccgcttcaat gctgattttg cttatactat ggatggtagt
     6301 caatatggag aattacaata tgaaagcttt aacgctgctg aagcggttat tacatgccac
     6361 ggtgtaaatg ttcatcctgg ttcagctaaa aatgcaatgg taaacgcaat acgtttaggt
     6421 gaacaattcg atagtttgct acctgatagt gaagttccgg agcgaacaga aggatacgaa
     6481 ggcttttatc acttaatgaa ctttgaagga actgttgaaa aagcaacttt gcaatacatt
     6541 attcgtgatc atgataaaaa acaattcgaa ttgcgtaaga aacgtatttt agaaatacgt
     6601 gacgatatca atgcccattt tgaaaattat ccagttaaag ttgatatatc ggatcaatat
     6661 ttcaatatgg cagaaaaaat attaccattg cctcatatta ttgatatacc taaacgtgtc
     6721 tttgccaaat tagatattcc agcaaacact gaacctattc gcggtggtac agatggttca
     6781 caattgtcat ttatggggtt accaactcca aacatcttta caggttgcgg taacttccac
     6841 ggcccatatg aatatgcatc cattgatgtt atggaaaaag cagtacaagt aatcattgga
     6901 attgtagaag atatcgctga aaatcactaa aaaccaacga ttatatacca actcaggctt
     6961 aattaataaa aaaatcacgc taacattcag tgcaatgata gcgtgatttt tcatattttt
     7021 tacgtatact ttcgatcttc taattcgttt tgctaaccat cataaatgtt attaaccaac
     7081 atgacagcat caccaattta gtccttcttt actaaacctc aacattaaaa tgtgttttca
     7141 acagttttaa agactgaacc gtataatgcc taatttgtaa ccccatttta aagtcagtat
     7201 aagtttctgg catatcgata aatgtattaa acattgctgt aaccccttgt gattcaaata
     7261 aatcaaactt ttcagcagtt gcacaaattg caatggcaac cttttgatgt ttatgacaaa
     7321 gttctgcaat acgcaatgtt gtcgtttcta gcaactgatc attttcattt aacccttctc
     7381 caaaaataat taaatccgct tgttcaacta aattttctaa atgtgttagt tggtctacta
     7441 atgcatgact ggttaatatt tcagcttgat acagtccatt caagactgct gcaattccac
     7501 caccagcacc accacgttca actggaccaa ttgcaatttt caattcactt ttaaataact
     7561 cactaaaata ccaaattaaa ttatcgattt ctgctgcttg attatgattc aactgatgcg
     7621 catcataagt ttgcatgatt tcactttgct taccatataa tcgacttgaa aaatccgaca
     7681 ttacttgaat tcttgctgtt tccattttag ggtgtaagtt cgacatatcc atacgacgaa
     7741 tatatttaat tacaccagca ccttgtctca tatctacgac acgcccttcg tcatcataga
     7801 attgagcacc taatgcttgt aacatacctg caccagcatc aaaactatca atcccaccta
     7861 gtgaaattac aacatgtttt gcgtcgttat ctaatgcatg tttaatcatt tctcctaaac
     7921 cataacttgt tcgttcaaca attggttttt taccttttaa aaataaattc ccctcgataa
     7981 cggtcatccc tgtatcagtt tgtccgtaaa caccttcaac ttcattcata tctgcatcat
     8041 gtactggtat acgatacttt tgcccagatt gccataaaaa tacagaatct aataattcat
     8101 gtcttccatt aaacaatggt acttgaacta catctgcagt ttcaatttgg cttgcaactg
     8161 cctcttcaac atatctatta gcttgataac tcgaaataat tccatgaaac tcatccatgg
     8221 ctactaatac tttcataacc ttgaccacct tactttatta tttgttgtga tacgtttttt
     8281 attcataaaa gattaatctt aaattttaga ttttatctcg tgattatatg cgaacattta
     8341 acatgacatg attattaatt attccagtaa ataacaatat gcttcttttg gtttagcata
     8401 tatttcctat taaactaact tacaaatcat gtttaaattt tcttattgga ttcgtcgttt
     8461 tccaaaatat tttcaataaa aatactcgaa tacattatac atatgtttgt aaaaaagtac
     8521 taacttaact gcttctaaag gtgacataaa ataaaaggtc agaacatcat tatagccaaa
     8581 ctactctgat tcacacgaat ttaagttgtg ctattcacgt ctagtagtgt aaggcaaaat
     8641 gttccaaccc tatagtttca ttattcttct gcttgtgcta atgacgacac attgatatca
     8701 ccgtggtctc gattccaaac catttcacca tttacaaaat aaaatgcttg aggtgattca
     8761 tgtttaacgt tcgttttttt agcaatataa tctgacaaat cgcgttcttg ttggacaatc
     8821 aaataataac cgtccatatc gcgttcatat aaaaatttat taaattgatc gtacgcattt
     8881 gccgatattg gacaagtttc actatgtttt aatacaaaaa catatttatt ttcctcaata
     8941 acctgttcaa attggtcaat tgaacttagc tttatagcca ctctattcac ctctaaaaat
     9001 atttatcaca tttaaatagc atctgcgtga catttttata catatagtta atatcaatgt
     9061 ttaccattat acacatagta agttgacatg ttaaccataa attcaaaatt tttaactttg
     9121 aaaaatgatt atagttattt ttcttctaat ttttcaagct ttttttcaaa ggctttatcg
     9181 ccgtgagtta tatctatacc ctttttcggc aacttatcta catcgacttt tgataacttc
     9241 tcactaacct ttattgtttc aatacgtgta ttataatagt tctgcagact gtaatacatt
     9301 tctattgcgt ttttccttgc attattaaca tgcttatcac taatattagt ttggttaata
     9361 tcggtaattt gcttttcaat cattggcata atgtgatttt taatcgcgcc ttttacttca
     9421 ttttctttac tatcatctag gttcttcttc gcagtatctc ttaacgcttt attattatgt
     9481 tctaattttg tcgtaagatt agttgcttca cttttattat cagctaattt aatttctgat
     9541 tcaactttgt atctattttt ttcaaattgt ttcgtataat ctaaaatgtc ttcgttatac
     9601 ttgatagatt gattacataa acctatgaat ttttttaatt gatatatcgc atccttcttc
     9661 tcatttgcaa gcgtcatata ttcttttttt aatttcttaa ctttcattgt atcatcaggc
     9721 aaattttttg ctgacttata atatgcttca aatttaggta tgagatggtt tttaacatct
     9781 tcttgtagtg ccttaaattc tttactattt ttatcagttg tatcagtttt acttagatga
     9841 tcaatttctt tcaaatgtat attatccatg acatgttgta attctttttg tttttgctca
     9901 acatctttaa attgcttagt aaattgatgt tgtgcctctt tttctttatt accacaagca
     9961 cttattacta caaataaaat cgctaaaaca gcgataataa caattttttt catgattgca
    10021 ctccttaaca gattcattat acaaaaacat gtttattatg aaaatgaatt atgttatagt
    10081 attccagact ttatcgtgat taaattaaat ttcaatatca aaggagttta tcatgtattc
    10141 aaatgttcaa ccattcatta atagtgattt aactaattgt tttcaatcac aatacactga
    10201 aattgaatca ttattattca acctcttaga cagcccagta aaatatacaa aacatattgg
    10261 cggtacgcat cattttaaat attcaacaga gccaatttta gatattttag taggtgtaga
    10321 aaatttgcat gatattacat ccttagatga aaaacgctta aattatgtag ggttttatcg
    10381 cttgcatcat aaatataata agaaagttat gatggcgaag tttaacaatt ttcatgaact
    10441 aaaacaacag gtgcgtctac atatcattca aatgaatacc caaacatttg aatcttatct
    10501 tgcacttgat tacctcttat caactaatga tgatattgct ttgcaattcc atacaaaaaa
    10561 gcaccaaata ttgcaacata gcttaacaat tagacaatat gagatgcaaa agcaacaata
    10621 ctttgaaaaa ctatgtaaaa aatacttata acgacaataa ttccgtattt tataattata
    10681 ctaattctaa tactttcttt tcaattttcg caaatgaatt ttaaaattgg tataatacta
    10741 tatgatatta aagacatgag aaaggatgta ctgagaagtg ataaataaag acatctatca
    10801 agctttacaa caacttatcc caaatgaaaa aattaaagtt gatgaacctt taaaacgata
    10861 cacttatact aaaacaggtg gtaatgccga cttttatatt acccctacta aaaatgaaga
    10921 agtacaagca gttgttaaat atgcctatca aaatgagatt cctgttacat atttaggaaa
    10981 tggctcaaat attattatcc gtgaaggtgg tattcgtggc attgtaatta gtttattatc
    11041 actagatcat atcgaagtat ctgatgatgc gataatagcc ggtagcggcg ctgcaattat
    11101 tgatgtctca cgtgttgctc gtgattacgc acttactggc cttgaatttg catgtggtat
    11161 tccaggttca atcggtggtg cagtgtatat gaatgctggc gcttatggtg gcgaagttaa
    11221 agattgtata gactatgcgc tttgcgtaaa cgaacaaggc tcgttaatta aacttacaac
    11281 aaaagaatta gagttagatt atcgtaatag cattattcaa aaagaacact tagttgtatt
    11341 agaagctgca tttactttag ctcctggtaa aatgactgaa atacaagcta aaatggatga
    11401 tttaacagaa cgtagagaat ctaaacaacc tttagagtat ccttcatgtg gtagtgtatt
    11461 ccaaagaccg cctggtcatt ttgcaggtaa attgatacaa gattctaatt tgcaaggtca
    11521 ccgtattggc ggcgttgaag tttcaactaa acacgctggt tttatggtaa atgtagacaa
    11581 tggaactgct acagattatg aaaaccttat tcattatgta caaaagaccg tcaaagaaaa
    11641 atttggcatt gaattaaatc gtgaagttcg cattattggt gaacatccaa aggaatcgta
    11701 agttaaggag ctttgtctat gcctaaagtt tatggttcat taatcgatac tgaaacacgc
    11761 tgtcgccatt attttaccga agaagatatt attgctatta aatttaaatg ttgtaataaa
    11821 tactatccat gctataagtg ccataatgag tttgaaaagc acgccattaa gcgttggtct
    11881 gagccttcat ttaacgaaaa agcaatctta tgcggtgtat gcaaacacga attaacaatc
    11941 aatgaatata tgatggtaga gcgttgtcca aattgccaat ctcggtttaa caatcgctgt
    12001 aaatatcact atcatattta ttttgaaatt taaacaacat agtcaaaaca ttatagggag
    12061 tgggtcagac atgataaagc accacttctg tagaattcct ttttgaaatt ctctatgttt
    12121 gggccccgcc aacttgcatt gtcagtagaa attgaaaatc aaatttctct atgtttggag
    12181 cccactggta ttgaaatgaa cttgttacaa gtacatgttc attcaaccaa cgactgacaa
    12241 cagaacattt tagagtctag gacattgatt gacgtcccag atttcctttt ttaaagaaaa
    12301 gaggggagca atgattaaga cctttaacca ttgttcccct cttttaaaat tattttacaa
    12361 ctttatcaag ttcctcaatt tgtttaattg tagttgtagt tgatccagct gcaaagtacc
    12421 ataatttagg atctaaatta tatactttgt cttctttaat tgctttaaca ttttttaata
    12481 caggattatt taatgcttgt ttcgcagttg atttaccact tatcgcttga cctctatcca
    12541 tcgctaaaat aacatctgga ttttctttat taacatattc gttagaaaca ttttgtccat
    12601 gattgctatt acttactttt ttatcaactg cattgaatcc taatgtatcg taaactaatc
    12661 caccaaaacg acctttaggt ccaaatgttg ataattcacc ttcgttaact agtaaataca
    12721 taacagtttt attgaagttt ttcgttttat ctttcattga agcaattttg ttatctaaat
    12781 ctttatttaa ttctttagct ttatcttctt tatcgtaaat tttaccgata ttttcagtgt
    12841 tttgtttcat tgaaccaatt aagttctttt catctgcacc aacataaaca attttcgctt
    12901 taggtgcagc ttttttgaat tcatctaaat tcttttgatt agctgtacgt ccagagataa
    12961 agattacttc gggtttcgtc gcagcaattt tatcaaaatt cacttctttt aaattaccaa
    13021 cgtttgtata tttatcatct ttaaatgatt ctaagaaatt cggtaatgac ttaccgcctt
    13081 cccctttagg taatgctttt actttatctg ataagcccat ttctttcatt acatctaatg
    13141 cgccatagtc taacacaact gcatttttag gattttttgg tacttcaaca gtttctttaa
    13201 cttttttagc gtcactacca tcttttttct cgccacgcat tttatagtta ttttcaattt
    13261 ttactgtatc tttagaacct ttagtttctg attttgattg ttctttatca gaattgttac
    13321 cgcatgccgc taataaaaac attactgcta atactaaata taagactgtt ttcttcataa
    13381 tataatattc tcctcttttg gcttaatctg tgtgcttgat gtgaaatata aataaatgct
    13441 ttacgtttaa cccttcttta attggcgcat caccccttta aagtgtctcc aaattatcaa
    13501 tccttaaaat ataaacaaat cctttgattt cttatatgtt caatttgaat attcatgtca
    13561 tataaatcac ttaaaacact actttgaatg acattatctt tcaaatctga cttaactagt
    13621 tgtccgtttt tcaatgctac aatctgatct gaataacagg acgcaaagtt aatatcatgt
    13681 aacacaatga caatcgcttt attcatttta tgcgctaaca tttttaacgt ttgcataatt
    13741 tgaacagcat gcttcatatc taaattattt aatggttcat ctagcaaaat atattcagta
    13801 tcttgtgcta ttgtcattgc aatgtatgca cgctgacgtt gtccaccaga taatgactta
    13861 atattacgat ttctgatatc ttgtagttgc aacaaatcta gcgcatcatt gacaatatca
    13921 tgatcctctt tcgtcaaacg acctttagaa taagggaatc gtccaaagtt taccaactgc
    13981 tctaccgtaa tattcatttc agtatggttt gtttgtttta atatagatat ttttttcgac
    14041 aagtcattat ttttataatc agacatgagc tgtccatcta ttttcacttc accgttatca
    14101 aaacgaatta acctacatat cgctgaaagt aaagtactct tacccgcacc attaggtcca
    14161 attaaagaag tcaatttacc tttttcgata tctatgctaa tatcttccaa tatcatttga
    14221 ttatttatag ttttagttaa attttcaact tgaatcattg cgcatttctc cttctaacta
    14281 atagataaat gaaataactt ccaccaatca aatcaataag tatactcatt tctgtcgtag
    14341 cttcaaacac attttcaact acccattgcg cactaaataa actaatccaa ctcaaacaaa
    14401 ttgtcgcaat taaaatatac ttatgttcat acgtcttcat tagttcatgc gctagattta
    14461 cagttaataa acctaaaaat gtaattggtc ctactaatgc agttgacaca gaaactaaaa
    14521 ctgagactat tacaagtaga attcgcgtta atttttcata cgatatccca agattaattg
    14581 cttcagcacg acctaaaagc aatacatcta aataaggcaa tagtaaaatt gtaatgacta
    14641 ataagattac taatagcact gctgagaaaa taactaaatt cgaattagaa gcattaaaat
    14701 tagcaaacat actactttgt attgctaaaa atgactcagg atccataatc agttgaataa
    14761 aacctgttat gcttctaaaa aatgttccta aaaggacacc aataagtaag ataaaataaa
    14821 ttgaaaaatg tccaatttta aagatacctt ggaataggat tagtgcgaat aacaccatcg
    14881 ttataagtgt taatataaag ttcaaatata cattagtaac ccataccgat tgaattccaa
    14941 ataaaaagac tggcaatact ttgataaata aataaactgc atctaacccc attattgatg
    15001 gtgtcaatag acggttattt gtaatcgctt gaaaaatcac cactgaagtt gcaatggcag
    15061 cacctactaa aataattaat atgaactttc ttaaacgact tgaaaattga tattcaaata
    15121 tttcaaaatc aatacctaca aataagtaca gcatactaat taataacgtt actgcaatta
    15181 aaagcattat ttttttatta ttgttgcgca taatttttcc tacctttcat aagcaagata
    15241 aggaaaatga ttgttccaaa tacacctatt gttaaaccaa tatttatttc atatggataa
    15301 acaacaattc tgccaactat atcagaaaat aatacaaaga tggcacctaa catcatcgta
    15361 tgagggatag catttttcaa atgatcacct cgataaattg aaataatatt tggtattact
    15421 agtcctaaga acggtaatgt tccaacagtc accactacca atgctgtaat agttgcagta
    15481 ataaacaatg cgatgttaat taatttttcg taactcaaac ctaaattatt agtaaagtct
    15541 ttacccattc ctacaatcgt gaaatgatta gcaaaaagat atgtcaatgc taaaagagga
    15601 atacttaaat ataaaatttc atagcgtcca cttgtgataa tggcaaagtt cccgttaagc
    15661 cagttaccaa tgctttgaac agcattcgtg cgcaatgaga tgaaggttgt gaaacttgca
    15721 acaatcccac ccatcataat tcctagaagc ggtacaaaaa tgacatcttt cacttttata
    15781 aaatcaatga ttttaacaaa taaaaacgta ccgcaaatac tgcaaataac agcaaatact
    15841 agttttaata aaatatgacc ggttggaaag aacaataaag caattaaaat acctagttta
    15901 gcccattcca tcgttccagc tgtagtcgga ctaacaaact tattttgcat catttgttgc
    15961 attatcaagc ctgctaaagc caacgaactt ccagcaatta aaatactcat cgtccttggt
    16021 attcggcttg agaataaaat attccgctgt gactcagtta aatgtagtaa atctttaatt
    16081 gatagttcac tcacacctat gaacaacgaa ataattgtca acaatattag taatagaagc
    16141 aaaatatttc cctttaataa atacttcatt acaaattttt ctccctttaa attaatgata
    16201 atcattatca ttgataatat ttctcattaa tagtagcgtt ttttgaccat aatttcaata
    16261 gcctttcaaa taatttagtg tcaattttat tgatatttgc aattaaactg aaatttaatt
    16321 tttcggaatg tgtattttgt ttgcttaaag taaaatagaa cacttaagat tttgatgtct
    16381 gggaatagtg gaaatgataa aaactactaa tgattgatta tgtagtggtt cttaacatta
    16441 gcctcagcta atgtgtactt aaaaatagga atacatgagt aaaactcatg tggaagtgat
    16501 aatttctaaa ggaaaaagtt tttctttatg ttggggcccc gcccgaactt gcacaatatt
    16561 gttagctgac tttccgcctg cttttatgtt ggggccccgc caacttgcac attattgtta
    16621 gctgactttc cgtctgcttc tatgttgggg ccccgccaac ttgcacaata ttgttagctg
    16681 actttccgcc tgcttctgtg ttggggcccc gccaactact gccaatataa ataacattgt
    16741 acatcttaga gcattgattt cttactcaga ctcataatga gttagataca cataaaaaaa
    16801 gaccttcaac atgtaggaga caagacgaaa cgaatcgcta tttcccttta catgtgaagg
    16861 tcttttaatt taattgttat ttgttgtcaa atacaaagtc atcatcttgt aatgcttcta
    16921 cgtttaatgc taatacataa ccatcacctt ttactgagaa gaagtcatgg tttttagttg
    16981 ttgtatctaa ggcattctca atgattgggt taaattcacg ttcctcaaaa taaggttcaa
    17041 agcctaagtt tgaaagtgct ttgtttccat tatatttaac atagtttagc acatcttcag
    17101 tgattccaag atcatcgtat aacatttttg tgtatgactc ttcatttaaa tacaagtcat
    17161 ttagcaattt atacatttct tgatctgctt tttgtttctc actttcagat agttcatttc
    17221 gtaaatgctg tgcatctaaa ccggtaaata caccatgaat agattcatct aaaagaattt
    17281 tacgaatgat ttcacctgat gtcgtcattt tcccttgacc agcaagatat agtggataat
    17341 agaaacctga gaagaataag aatgtttcta aaaatacact cgtaactctg gccatatatt
    17401 ggtcgtatat cgaagcttct ttaccccaaa gtttgtgata attagcaaca attttatcag
    17461 atttatattt taaatggggt tcctctaaaa cccattcatc taataggtag tttgtttcac
    17521 tagatggtaa tagtgttgtg aaaatatgtg aatagctttt cgcgtgtatt tgctccatca
    17581 tcgccataaa tgaataaact gcttttttcc ttaagtcagt cgtatgtagc ataactaaag
    17641 gcatgccatc atctgcttga tgtgtatcta agcctgttaa accagctaat gcttttttaa
    17701 atgtgtcttg ttcagcttca gataaagtct tccaacttgc aatgtctttt gatactttaa
    17761 attctgtttc aacccacatt tgagatatat tttgtctcca aaacatattc gtcatatctt
    17821 cttgtgtgtt ccaattaaca gctatcatga atataatttc ctcctgtaat gttactcaat
    17881 aattgacaaa tacagacata gcagtctaat catctgctaa ttcagaccag atgattagct
    17941 gtttgtcatt ttcaacattt aattgttaga tagaacaact tgtacattct tctacactta
    18001 ataatttatt tctagtatag taaagtgatt ttaatccttt atagtgcgca tatacatata
    18061 atcttgctaa ctcacgtgta gaaatttcag aattaacata aaggatcgtt gagatacctt
    18121 ggtcaatatg cgtttgaatt gtcgcaatta aatcgattaa tttcatctga tcagtattga
    18181 atgctgattt gtagtaccac attgtttgtg gtgataagaa tggcataggg taaaatgttt
    18241 ccgcattacc ataagtacga cgttcaattt ggtcaacgat tggcattaca gaacttgttg
    18301 cattttgaac ataagaaata ctttgtgttg gagcaattgc taatctatat gcatgatata
    18361 aaccatattg ttcaacatct tgttgtagtt tcttccaatc ctcagaagta ggaatagcca
    18421 taccatcgaa taattcacgt actttttcga attgaggttc aaattcttga gttgtataga
    18481 actcgaaata tttgccatta gcataatcag acttttcaaa gtcttgatat ttgataccac
    18541 gctctttagc gatttccatt gaacgttcga ttgagtagaa attcatcatc ataaagaaga
    18601 tatttgcaaa atcttttgct tcttctgact cataaccaat tttatttttt gctaggtaac
    18661 cgtgtaaatt catgacacca agaccaactg aatgtaattc actgttagct tttctaactc
    18721 ctggtgcatt ttgaatattt gctacatcac tcacaacagt taatgcgtcc ataccagagt
    18781 gaactgaatc tctgaatttt ccgctttcca ttacattaac aatatttaat gagcccaagt
    18841 tacaagaaat atcacgttta atttcgtctt caataccata gtcattaata attgaagttt
    18901 cttgtagttg gaaaatttcc gtacataagt tactcatttt aatttgtcca atgtttgaat
    18961 tcggatgcac tctgttagca ttatctttaa acattaaata tggataacct gattgtaatt
    19021 gtgtttgcgc aattaaattc aacatttcac gcgcattctt tttctttttc tcaacatttg
    19081 ggtttgcaac catgtcatca taatattttt ctaaatcgat atcgtctaat gtcacaccat
    19141 attcttcttt aactgtatga ggtgcaaaca tataaaagtc cttaccttct ttagctaaat
    19201 cgaagaattt agatggaaca attaaaccag ttgatattgt agataaacgt aaatcttcat
    19261 ccgcatttac ttttttagta tctaaaaatt cttctacatc ataatggaag atatttaagt
    19321 acacagcacc agcaccaggg cgttgaccaa gttgatctgc atagctaaag ccaccttcaa
    19381 gtgacttagc aataggtaaa acgcctttcg ctacgccttt aattccttta attgcttcac
    19441 cacgtgcacg caatttagat aagttaattg caacgccgcc cccaatttta cttaattgtt
    19501 ttgcagttga atcaataaag ttaattgaat ttaagctgtc atccacttct aataagaaac
    19561 atgacactag ctcaccacga cgcgcacggc ctgcgtttaa aaatgttggt gtcgctggtt
    19621 gatatctttg ttcaaccata gcagaaataa attgtttagc ttgtgcttta ttaccatttg
    19681 ctaggtataa agcaacaatg gcaacgtgtt gattatagtc ttctaagtat tgacttttat
    19741 catttgtttt caaagcgtaa tctttgaaaa atttactagc tgacatataa cttgcaaaat
    19801 taaacgggat tgattttgca taatcagtga tttcaattag atccgcttca ctataaatat
    19861 caaacacatt gaaataaaaa tcgttgtcta ctaaataacg taaacgctcg atttcagtgt
    19921 cgaagaagat tgttttgtct tttacttctt ctaaataagc tactaaagct tcttggtctt
    19981 tttctaaact aaagaatcca tcttctcttc gtttagtgac ctcattattt aattcaatat
    20041 gattgtactt tttctcttcc atggttttca ttaaaattac ccaccttgtt cttaaattca
    20101 ataacgtctt tgttttttcc atgtaactca aacttcatta ataaagggac attatactct
    20161 tctgatatcg tgcgacccgc ttttgcgaaa tttagtcccc aatttcgatt accgctagct
    20221 gccacacctc tgatgtattg atgattaact tctaaaaaag attgaacggg ttctggtact
    20281 tctccaaatc caatagtgcc agtaacgata ataaacggtt catgaactgg ttccatacaa
    20341 ttttctgctg taatctcaag cgtattttca agttctgttc tcttaataaa acgacggaca
    20401 tttccagtaa atgaaaaata tattattttc attggatcac cttctttctt caaccctttt
    20461 aaataaagta gtcattccaa ttaaaaaatg ctttaacaac atggttacta tggttatctc
    20521 agcattgtat taactataat gaagctactt attttctcaa atgtaaacca attgcataga
    20581 tttatatttg agaaaatagt agcttatatg ggttgaatgt ataaaaatca ctatatgtag
    20641 tatttacatc attggatatc ttccatttgc tcagtacatt ttctaaaaat atgactatta
    20701 attgataata actattaaat ttctgtgtta aattactttt aaatgtaatt tcttcataaa
    20761 taaagcattt gcatacaaaa cacaagatat tgtgtctttt tcgtttcacc aacactatat
    20821 tatgtgtttc attatacata agacacacta tgatttaaag tgccattttc taatcgaatg
    20881 ttcgtaaaac gttccaacta ggcaccaata ctaaattaga aaaacttgaa caaatttaaa
    20941 aaaatttttt agtagatatc acttgatttt tttaaatctg tgtcaatctc aattgtatca
    21001 acagaaatca agaaaaagct tattagcttg atactgaatt gatatttgtt ttatgtaata
    21061 acacaaacat tcataaaagt gtaatgccct attattccga caaataaaca cagaacattt
    21121 gttcggtaca gtctattata gtctttttat agtaaatttc aactatttat gcacaacatg
    21181 ttgttttatg ctaatataaa tacgttaatt ttatataaaa gaggcgataa aattgaatcc
    21241 taaagtaaaa ggtatcattg ccattttaat ttcggctatt ggtttcagtt ttatgtcagt
    21301 gtttttcaga cttgcaggtg accttcctgt ttttcaaaaa tcattagcac gaaacttagt
    21361 agcaatgttt attccattat tttttatata taaataccat cagccaatgt ttggcagact
    21421 ttcaagtcaa cccttattaa taacacgatc aacacttgga cttattggtg tattgttaaa
    21481 tatttatgcc attgatcata tggtacttag tgatgccgat tcattaatga aactcaatcc
    21541 tttctggacg attttattaa gcattgtttt cttacatgaa aaggtaagga agtatcaaat
    21601 tacagcaatg ataattgcta ttttaggtat gcttttaata gtgaaacctg aattttcatc
    21661 atctatgatt ccatcattag ctggcttatt ctcaggtatt tttgcagcat cagcatatac
    21721 atgtgttcga gcacttagta caagagaagc accatatact atagtatttt acttctcctt
    21781 attttctgtc atcgttttaa tacctttcac tgcatatacc tatgaaccaa tgagtcagat
    21841 gcaaatactt tatctattag gggctggctt agctgccgct gtgggtcaaa ttggtgttac
    21901 attagcatat agttttgcag cagcaaaaga tatttctatt ttcacgtatg catctataat
    21961 tttcacagca atacttggat ttattctatt cggagaatct ccagatttct atgcaacatt
    22021 aggttacgtc gttattatcg gtgcaagtta ttacatgttt gaaaaagcac gccgagacgc
    22081 caaaataata aaaaaataaa tttaaaggag cgctttatat ggcacatggc cgtcaacaag
    22141 atgaattaca agatataact ttattaggaa atcaagacaa tacttacaat tttgactatc
    22201 gtcctgatgt attagaatcg tttgataata agcatcaagg tcgtgactat ttcgtaaaat
    22261 tcaactgtcc agaatttaca tcattatgtc caattactgg gcaaccagat tttgcaacta
    22321 tctatatttc atatattcca aatgttaaaa tggttgaatc aaaatctttg aaattatact
    22381 tatttagttt cagaaatcac ggtgattttc acgaagattg tatgaatatt attatgaatg
    22441 atttgataga gcttatggac ccacattata ttgaagtctg gggtaagttc acaccacgtg
    22501 gtggaatttc tattgatcct tatacaaact atggacgtcc aaattctaaa tatgaaaaaa
    22561 tggctgagca tcgtttgatg aatcatgatt tatatcccga aaaaatagat aatcgttaaa
    22621 tgtatcattt aataaacaca ccaataagtt gattttccta acttattggt gtgtttttca
    22681 tttagcatac ataataggtt acattaaaat aacattttat accaaagtac accaaaagaa
    22741 tattagtaca cgaattaaac aacattttta tagaaaccta ttgcacttta acgtcaataa
    22801 gtatattttt atattatctc taattaattg tgcgcgctta ataacagaat attctcaata
    22861 tttttatttt ttgtgatttg ttggaatatt tagttgataa ggcacaatca aatttactta
    22921 aactattgta ttaggggaag aaaggatggg atgtatacat gacacaacaa aactcccatg
    22981 gaaatcaaat tcaagacata cctcaaacag gatttttcgg gcatcctcga ggactaggcg
    23041 tactcttctt tgtagagttc tgggaaaggt ttagttatta tggcatgcgt gccctactca
    23101 ttttctacat gtactttgcc gtaacagata atggccttgg aattgataaa acaacagcta
    23161 tgtcaattat gtcagtttat ggttcattaa tctatatgac atcaatacca ggcggatgga
    23221 ttgctgacag aattacaggt actagaggcg ctactttatt aggtgcagtc tttattatta
    23281 tcggacatat ttgtttaagc ttaccatttg cattaatcgg cttattcaca tcaatgttct
    23341 tcatcattat tggttcaggt ttaatgaagc caaacatttc aaatatcgtt ggccgtttat
    23401 atcctgaaaa tgatagacgt atggatgcag gttttgttat tttctatatg tcagttaata
    23461 tgggtgcatt attatcacct attattttgc aacactttgt taatgttaaa aacttccacg
    23521 gcggattctt gattgcagca gttggtatgg cattaggttt agtatggtat gtacttttca
    23581 accgcaaaaa cttaggtagc gttggtatga aacctactaa cccattgaca ccagctgaaa
    23641 agaaaaagta tggtcttatt atcggaagtg ttgttttagc aattgtatta attatcgtta
    23701 ttggtgcatt aactaactca ttatcattta acttagttag taatactgtt ttagtattag
    23761 gtattgcatt accaatcatt tacttcactt taattattag aagtaaagat gtcactgata
    23821 ctgaacgttc tcgtgttaaa gcatttattc cattatttat tcttggaatg gtgttctggg
    23881 ctattcaaga acaagggtct aatgtattaa acatatatgg aattgaacat tcagatatga
    23941 aattaaactt atttggttgg aaaacaaact ttggtgaagc gatcttccaa tcaattaacc
    24001 cattatttat tttattacta gctccaatta tttcactttt atggcaaaag cttggaacta
    24061 aacaacctag cctgccagta aaatttgcaa ttggtacgtt cttagcaggt gcgtcataca
    24121 tactaattgg tattgtaggt tatgcatcag gttcatcaaa cttttcagtt aactgggtta
    24181 ttttatcgta cattatttgt gttatcggtg agctatgctt atcaccaacg ggtaatagtg
    24241 ctgctgttaa gttagcacct aaagcattta atgcccaaat gatgagtatt tggtacttaa
    24301 ctaacgcttc tgcacaagca atcaatggta ctttagttaa gttaatagaa ccactaggtc
    24361 aaacaaatta ctttattttc ctaggtgttg ttgcaattat tgttacaaca attgtattag
    24421 cattctcacc tttaatcatc aaagcgatga aaggtatacg ttaatattgt tggcctaatt
    24481 caaaaaacag taagtcattt aaatggctta ctgttttttt ataggtttct attaattaaa
    24541 ttcaagatat cagtataaat aaaagcttaa tatgctcgtt atagacagct ataactatat
    24601 tttctcgtcc cactctatag acatcaatgt cagttattac cattttctct atttaaaaca
    24661 tatttttggt attaacaatt aaaagtgggt atatatattt aatgaaccaa tttttagaaa
    24721 gtaggctaaa ctatggaaaa taaatataca catggtgtcc tcttttacca tgaacacagt
    24781 ggattaaaaa atattaatca aggtattggg gaagttacaa cagcattaag ttcaatttgt
    24841 aagcatcttt ctattcaatt gagtgaaaac gaaggtgaca ttattaaata ttgccaagaa
    24901 atcaaaacta aaaactatgc aaaagatgta gatatattat ttattcttgg cggtgatggc
    24961 acggttaacg aacttataaa cggtgttatg acacatgacc ttcaactccc tattggcatt
    25021 ttaccaggcg gtacttttaa tgattttaca aaaacgctaa atatagcacc taatcataaa
    25081 caagctagtg aacaaatgat ttctgcacaa gttggtaaat acgatgtaat taaaataaat
    25141 aatcaatatg cactcaactt tgttggctta ggtctcattg ttcaaaacgc tgaaaacgta
    25201 caagatggtt caaaagatat atttggtaaa ttgagttata ttggatcaac tgtcaaaaca
    25261 ctgctaaatc caacacaatt taattatcaa ttatcaatag atgataagac atattctggc
    25321 gaaacgacca tgatactaac agcaaatggt ccattcattg gtggtagtcg tataccattg
    25381 actgatttgt ctcctcaaga tggtgaactg aacactttca tttttaatga acaaagtttc
    25441 agtattttaa atgatatttt taaaaagcgt gatagcatga attggaatga aattactcaa
    25501 ggtattgagc atatacctgg taaaaagatt tcacttacaa cagatccagc aatgaaagtt
    25561 gatattgatg gcgaaatttc attagaaaca cctattgata ttgaggttat accaaatgcc
    25621 attcagttac ttactgtcaa tgatttgtaa gcatccggaa tgatcttaac ccgatattat
    25681 attaggtttg gagtgtaatc ataagattca acgcacctta tattcgtttt tattagcttt
    25741 aagctattac cctgtttcaa aaacagacat ataattttgg cgcatgccac taaaatgatt
    25801 gatgttgtat tttaaatcat agtggtttat gcgtcttttt caaattctat aaaaaatcgg
    25861 atgacgtgta atctgccata gattaacaca ttcatccgat ttataataat aagatagact
    25921 aacatttatt gagagcggga cggaaatgat aaagaacgac taatgattga ttatgtagcg
    25981 attctttatc attagtcaca gctaatgtgt acttaaaaat atgaatgcat gagttacact
    26041 catgcataag aaatactaat ttctaaagaa aaagtatttc tttatgttgg ggcccacccc
    26101 aacttgcatt gtctgtagaa attggcgttc caatttcttt atgttggggc cccgcagtca
    26161 actactgcca atatagtgtt gtagtgtcta agacataaaa gttcaaccaa gtcacaatta
    26221 ctttatagcc tatccatcat ctttactaca tgaaattttt caagtgatat atttttattt
    26281 ctcaatatta tctaaaaaat actgttctac atctttccag ctatttacgc gttcaaatcg
    26341 atcatcatta atattatgca ctgctgtaaa cataatcggt gtaccagtaa aaatttcaag
    26401 ctgtctagga ttgtcatcta ttaaataatc agctttaacg atgttttttc taccacaaaa
    26461 aacaaaatgc tgaggatcta aaaatggaaa gaactctagt aaccattcat atttatcact
    26521 aaatgatgtt ggtacatcca ttgctgctgt agcaatatat acatcataat gttcagttaa
    26581 ttttttcaca acttcttgtg catacggcat cactttaaga tgtctgaaga agcctggttc
    26641 tctcaatact tctgtaatta atccatcatg ttcaggaata acatgtttaa gtttttgacc
    26701 attcaaagct tccattttaa tacctaaatc cgctctaaaa ttgacagcat caatgatttc
    26761 tcctaatgta tctgccaata cttcatccat atcaatcgcg attgatttac gggtcatttt
    26821 aatcttctcc tcaaaatatc agtattgatt cagtatagca aaaaagcaat tgatacaaaa
    26881 caatatcatc tattgttgct ttttacactg aatactctca cctcatcatt cacactgcat
    26941 tgggagtggg acagaaatga tattttcgca aaatttattt cgtcgtccca ccccggcaag
    27001 gttgactaga attgaaaaaa gcttgttaca agcgcatttt cgttcagtca actactgcca
    27061 atataacttc gtagagcaca gaacattgat ttatgtccca gcctgcactt actattcgta
    27121 tttaaagttt gataaaactt ctaacatttt atcattttgt tctttaaaac caattgtaat
    27181 tctaacacca gttggaaatg gtcgcgtaat acaccctaca tttaaaagtg cttcataaag
    27241 ttcatttacc cgctttgttt ttacaaatat aaaattcgtt tgacttggca agaaatactc
    27301 actttgaggt aattgataaa atctttcgcg ttcaacacta tttttatgtg ttacctcttt
    27361 taaatattgt tgatcttcaa gtgctgcaac tgctgcgtat tcagatatac gtgtcacatt
    27421 aaatggtggt ctaatgatgt tccatttttc aatcgcatgt tcacttgcta ccacatatcc
    27481 tacacgtaaa cccgctaatc catacgcctt tgaaaatgta cgtaataaga aagcattgtc
    27541 atatttttgt tgcaaagcaa gtgtatccgg gtagtcctct tctgtcacaa attcaaaata
    27601 agcttcatct ataattactg gtacatgtgg aggtacttga gataaaaacg aatctaagct
    27661 ctcatgatta aaatatgtac ctgtaggatt atttggatta cataaccata ccaatgacgt
    27721 atcttcatta acttctttta aaataccttc taaatcgaag ccaccatctt ttaaaggtac
    27781 ttgtatcaca ttagctgatt caacaatcgc attgtgataa tattgaccga atgtcgcttc
    27841 acttgtaaca atagtatccc ctggcgttaa tacagctcta gaaatcatta atataacttc
    27901 atctaatccc gcaccaaata aaatgcgtga ttgatctaca tttaaatgtt tactaatcgc
    27961 cgcttttaat gtcggtgatc ctgtttcagg ataataatat aactcatcta agtgtgctga
    28021 tatcgcttct ttaactttag gcgatggtcc atacaaattt tcatttgatg caagtttata
    28081 taaatctcct tcaatgccat acttttcttt caatgccctt ggagataaac caggctgata
    28141 tgctgatagt tgattaagtt gttctttcat aataaaaaca acctccttag aattaactga
    28201 aattttaaaa aacttatatt cattatatcg ctctatgttt ctaaaatgca atgctactat
    28261 tttcacatca taatagctta cattgtgttt aacatgtcat attttttaat cattattgca
    28321 tgtttaaatt gaacataatt atagatttaa atataacaac gccacataag atataatttg
    28381 gtgttacgca tcttatgtgg cgttggtcgt tatttgatta acccttttgc ttttaaataa
    28441 tctttcgcaa ctgtatatgg gtcttcattt ttaactgtta ctttatagtt catcatttgc
    28501 atatcttcat cagatatttt gttttctagt ttgtttaacg gtttcttaat ttctggatgt
    28561 ttctttaaaa agctttcttt aaataatggt gctccttgat atggtggaaa tacgtgctta
    28621 tcatctttta acacaaccat atcatattgt tttaattcag cgtcagttga atatgcatct
    28681 attaaattaa tattaccttt attgatcgct tgatatctca acttaggttc cattgtacgt
    28741 atgttatcta aatttaaatt ataagccttt tgaacagctt tgtaaccatc tggacgatca
    28801 ttaaattcca atgtaaatcc tggtttaagt tgatctttaa ccttatttaa atcaccaatt
    28861 gtacgtatat tatgttgttt agcaaaatca cgttttacag ctaaagcata cgtattgtta
    28921 tacttcattg gttttaacat agtcatttga tatttctttt caagactttg cttagcttgt
    28981 tcataaactt ttttctcttc ttttgacttc aatggttctt ttgttaattc gcctaaaact
    29041 gttccagtaa attctaaata cccatctata tcgtcagatt ttaaagcatt aaataaaaat
    29101 gctgttttgc ccataccatc tttcacttct acagtatttt tggtctcttc ttctattaaa
    29161 attttataca tatttgtaat aatcgatggc tcggagccaa gctttccagc taacgtaatt
    29221 ttatcacctt tttgtgcaaa cataggaata gcgatagcca gtataataat catcactata
    29281 acacctaacg tcatcaataa ctttttataa gataatttag ccataaatct taaaattaaa
    29341 tcaaatataa ttgccaataa ggctgctgga attgcaccta ataatatcaa cgatgcattg
    29401 ttacggtcta tacctaataa aattaaatct cctagtccgc ctgcaccaat taatgctgct
    29461 agtgttgctg tacctataat taataccata gccgttctta caccagccat tataacaggc
    29521 attgctatcg gaagttcgac tttagttaaa cgtctaaatg gtttcatacc tataccttta
    29581 gccgcttcaa tgagtgatgg atcaacttct ttaattccag tatacgtatt ccttaaaatt
    29641 ggtaacaacg catacactac aagtgcaata attgctggca cacgaccgat accaaataaa
    29701 ggaatcatta aacctaataa tgccaacgat ggtatggttt gaagaattgc cgcaatattc
    29761 attacgattt cagatatcgt tttagtcttc gttaataaaa tacctaatgg taccgcaata
    29821 gcagttgcaa tcaataatgc gataaatgat atttgaatat gttctatcat tgtcgaaaag
    29881 agttgcccct tacgttcact caatatgtcg aaaaagttag tcatgttgag ctacctcctt
    29941 tttctgggac aaatatttga agatatcttt cctatcaata acatattgac ctacgctatc
    30001 ttcttgcatg acaatgacac gctcgctctc tgataaaagt tgatacaata cttcaattgg
    30061 ttgattgtca taaacaattg gataagcgct catagatgta acctcatcga ttggtttcat
    30121 aatatccaag tcacggataa ttgcgttctc ttcaacacat ggcgcaacat cttctaaatg
    30181 actacccata aattgtttaa caaattcact ttgaggatta tttttaaatc cttctggtgt
    30241 gtcaatttgt tcaatatgcc cttcattcaa aagacaaatc ttatcaccaa gtttcatcgc
    30301 ctcttgaata tcatgtgtaa caaatatgat tgtcttctta attttagttt gtaattcaat
    30361 taaatcatct tgaagttttt ctcggctgat tgggtctaat gcactaaacg gttcatccat
    30421 taaaataact ggtggatcag ctgctaacgc acgtataact cctacacgtt gtcgttgccc
    30481 ccctgacaat tcatcaggtt ttctgttttt atatttttca ggttctaatc caaccatttc
    30541 aagtaattca tctactcttt tatctatatc tttttctttc cactttttca tttgtggcac
    30601 ttgtgcaata ttttctttga ttgtcatatg tgggaataat gcaatctgct gcaatacgta
    30661 tccaatatcc caacgcattt cgtatactgg ataatcactt attggtttat ctttaaaata
    30721 aatataacct tcacttaagt gaatgagtcg attaatcatt tttaatgtcg tagtttttcc
    30781 acaacctgaa ggtccaatta gcacaaaaaa ttcaccctca ttaatattga aactaatgtt
    30841 atcgacagca acatgtttgc cataacgctt agttacattt ttaaacttaa tcactttgcc
    30901 acctcttttt ttctcatagc ataaaaccga gattatatgt atgtattccc tatttaacca
    30961 cgtttattac aattttcaaa tttaaatgat ttatccttga acttttttaa taaaataatg
    31021 aataataggt aatctccagt taagaaatag tgttatttta ccttgaattc aaaaaaacac
    31081 ccagtaaaac aaggaatgct tactaggtgt cttcactata ctttggcttt ataattttga
    31141 atcgtttcta aaaatgctgg acaataatgt tttaatttgt aactacctac gccatcaata
    31201 ttaatcatat cttgtttcga agcaggctta cgtttagcaa attcctccaa cgtgtaatca
    31261 gaaaatatac ttacaggtgc tatcgttaat ttgtcactta actttttacg aacttctacc
    31321 aactgactga ataatactcg gtcaacccct tcaaccgtat ttataaatac tttttcagtc
    31381 gctttttgct taaatggtgt tgtgaatact tctacttcat tactgagtaa ttttttaatt
    31441 gaagtatcac acattaatat ttcatcattt tcatttaaga accctttgaa tcttaattca
    31501 tctattaagt gacttaattc tgatgttgtg taacctttca ttaaaccatg ggttgaaatt
    31561 tgatcataac ctttgtactt aatataatct gttgactctc ctcttagcac ttgaatgata
    31621 acactataac tctcttgttg tttcatacga gcgatgcaac taataatcat cttagcttct
    31681 tgtgtcatat tatatgattt atcttgttga acacaattac tacattgttc acattcttct
    31741 aatttttcat tcggttcaaa ataatggaca attgttgctt ctaaacattt ttttgttttt
    31801 gtatattgaa tcattttagt taacttttcg cccattttat ctttatagtc atcatcagct
    31861 tgagagactg ttataaaata ctcgtgtaaa ttgatatcgc gttcgctaaa taacaaaata
    31921 cattcacttt tcaacccgtc acgacctgca cgaccggctt cttgataata agattctaaa
    31981 tctccaggca tattataatg aataacaaaa cgtacattgg atttatcaat acccatacca
    32041 aaagcatttg tagcaacgac tactttaaca cgatcaaata agaaatcatt ctgcgcttct
    32101 tctctttctt tattgctcaa acctgcatga tatataacac tttcaatttt ctggctttct
    32161 aaggcttctt gaagctcttc aacttgctta cgtgtagaac aataaataat acctgcatct
    32221 tcatcatgtg ttttaatata atccaatata aatttttgac gttgataagt aggatttact
    32281 ttaaaaatta agtttctacg cttagtactc gttttaattt gatcagtttg agcgatattt
    32341 aacttttctc taatatcttg ctgtacttca accgtggcag ttgctgtcaa cgctattatt
    32401 gtaaaatctt gaggtaacgt aaatactttt gaaataacat tttggtaact cggcctgaaa
    32461 tcatgacccc atttagaaat acaatgcgct tcatcaaacg cgactaagtg aatctttata
    32521 cgctgaagca tatttaaaaa atatcggttt tcaaatcgtt ctggtgcaac atacaaaaat
    32581 tgaatttctc catttgataa tgctttttca atacgttgtt gctctttttg agtcaaacta
    32641 ctatttaaaa aagcagcttg aattcccatc gcttttaatt gatccacttg atctttcatt
    32701 aatgatatta gtggacttat tacaattgtt gtaccaccta acaataaacc tggtacttga
    32761 tagcatatag acttacctcc accagttggt aagacaccaa gcacattacg atggtctaat
    32821 actttgctaa taatttcttc ttgtcctggt cgaaacgttt catacccaaa gtaatgcgat
    32881 aatgtttgtt gcatcataaa ttgacccctt attgttgttc tttaatttct tctaactcac
    32941 tccatcttgt gatgtctaaa tcatattgaa tttcaagttg ttctttttct tcgtttaatt
    33001 ctttaatttt cccataatct gcacttgcct caatcatgag cacatctatt tcttccattc
    33061 ttacttccgc ttgttctatg cgtttcatca attgttcata ttctaacttt tctttatatg
    33121 ataaaccatt tttcttacgt acagttgtag aagatttaga ttgttgcttc aatgtggatt
    33181 tatttttatc taatgatttt ttataacttt cataatcttc aaaagttccg ataatctttt
    33241 ccatctgacc atcatgaata aaccaatatg actgtgcaac tttatttaaa aagtagcgat
    33301 catggcttac ggtaatcact gtaccaccga aagtatgaat ataatcttca agtattgtta
    33361 aagtctcagt atctaaatca tttgtcggtt catctaacaa cagaacattt ggctggtgta
    33421 cgagtagacg taataaatac aaacgctttt gctctccacc agataattta taaacttttt
    33481 taccatgagt tgcacttgga aataaaaatc gttcaagaag ttgtgtaata gataccgagg
    33541 ttccatcttt ttcttttgcg atctcacttt cttctcttaa ataatcaatc atacgaatat
    33601 ctctatccag ggtctcatct gtttgcttaa aataagctac tttaaccgtc tgtccagtct
    33661 tcaatttacc ttcgaattgt tggtcttctc cactcaaaat atttaagagt gttgttttac
    33721 cagctccatt tggcccaaca acaccaatac gctcaccttt ttgaataatt tccgtcagat
    33781 gttcaaataa tactttatca ttaatagcct ttgataagtc ttctaattcg aacacttgct
    33841 tacctaatct tgaataagca agattcaatt cacctttatc gtctttatat tgctggttaa
    33901 cttcattttc taggtcatta aatcgattaa ttctagcttg ttgctttgta gtacgagcct
    33961 tagctccagc cctcatccaa gcaagttctt ccttatataa agctcgttgc ttttgttgtt
    34021 gcttttgaag tgatacttct ctttcagcgc gcatttcaat ataagattca tagttaccag
    34081 gatatgacgc taacttacct ctgtttagtt caataattct agtggaaact tcatttaaaa
    34141 aatatcgatc atgggttacg aataaaacag tatgaggata ttgcttcaca taattgatca
    34201 accagctgat tgattcgaag tctaaatggt tcgtaggttc atctaataac aataaatctg
    34261 gttgttctat taatgtttta gcaagtacaa cacgtttttg ttgtccgccg gataattctt
    34321 taatgtattt agtagtatca tgtataccta gttttgagag gattgtttta atttcagcgt
    34381 tatagtccca agcatcatgt tgatccatag catcttgcgc atccatcatt cgcttgaaca
    34441 atttatcact ttggtcatcc gcataagctt gtactgcctg ctcatattgc ttgatgatgc
    34501 gtaaagttgt tgtatcagaa cttaatactg catcaaaaac tgtcatatct tcattaaggt
    34561 cctgtttctg agacgaatat cgaattcgat attgatttgg atgcataaca ttggctgtaa
    34621 aatcatcatc aataccacca attactttta acaacgtact tttccctgta ccatttatgc
    34681 ctactaaacc tattttttca ccttctgaaa ttgataaatc taggttatcg aatatagtct
    34741 tatcggcata agatttattt aaatgttcaa ttttatatgc ttccataccc atcttccttc
    34801 ttctgttgaa atctctacgt acttaaatca gttctataca ttatacacct ttttgaaatc
    34861 aaaagaaatc gataccgtta agtgtcataa tttaatatat tgttgcttca actataaata
    34921 aaacttgtta aggctttgat gactatgcta atgatcttta taatatacct ttttatcaac
    34981 acatataatg taaaacataa tataaaagaa cataataaaa acccggattg atattttatc
    35041 aatccgagtt cgtgtttaaa tattattttt tagagtttgc tttaggtcct gtttcatatt
    35101 tatacttcga aggatttacc tttttgaagt ctggattttt gtagaatcta aacaaatcac
    35161 cattaagcac gttgtcactc atttcgagat ccttttcaac ttgcttttta ttcttttcga
    35221 aatcagctgg ttgagtagtt atgagttcat tatttttatt agaataaatc ttaccattaa
    35281 catatttata atcttttgtt ataaagtcac catttctgaa tggaactact tgattatgac
    35341 ctttagagaa taaatcagta ccgaacatta aataattctt cgtatctata ccagccaaat
    35401 gtaaaattgt tggcattaca tcgacttgac cagcatattc attattgata ccaccagatt
    35461 taccagggat tttaatccag aaaccagttc tgtttaaatc tgtaaattta gctggtgtga
    35521 ttttttcacc taatagtttt tccatggcat tgttatggtt ttcagagata ccatagtggt
    35581 caccataaat cataatcact gaattgtcat ataatccttt tttcttcaag tcattaatat
    35641 attcttctaa tgcttcgtct aaataacgcg ctgtttgaat ataaccatca actgttgcat
    35701 cacctgtgtt tgacttctca atagtcgcat ccttttcatc taatgtgaat ggatagtggt
    35761 tagtcaaagt aattaaatga gaatagaatg gtgatttcat cttagcttga taattagcag
    35821 aatctttaaa gaaaattttg tctttcaagc ctaagtttac aacgttttta tcagacatgt
    35881 catagtatgt tgcatcatag aatttatcga taccaaagtg tttatatact tggtctctgt
    35941 tccagaatgt tttatagtca ccgtgcatga catcagattt gtagccttgc ttttgatcta
    36001 aaattgctgg tagtgactga tacgtattat ctccttttaa tgaaaaggca gaaccttgcg
    36061 gtaaaccgta taaactgtta tccattgtaa attcagagtc agatgtttta ccttgacctg
    36121 tttgatggaa aaagttaggg aagtatgtga attgctcttt cccacttgat aatttgttta
    36181 aaaacggtgt tacttcttta ccattaacct ttttattaat taagaaggtt tggaaacttt
    36241 ctaaatgaat cttaataata tttttcttct ttgccacccc ataatattct gggttaggct
    36301 ctgtttgacg ttgtttcgta taatttaata cttttgttaa gtcatcttca gatgctaacg
    36361 ctttttgttg attattttcg atagttttaa caccatcgta tactgtaaag ttatacggtc
    36421 ctaaatattt cactaaatat ttatggtcaa atgtacgtgt taataattct ggtctgtcag
    36481 tttcagcaaa agctaagttt aagaagaata atgctactga agccgccatt acgacaggaa
    36541 caaatttctt actaaatgct tttgtgtcta accattttgt tttaaatatt aaaatgaata
    36601 agtaaactaa cgtatcaatg aaataaacaa agtcatacca tttgaatgat gcactaaccg
    36661 caccacccat agattctacg ttacctactt ggtttaaagt actaaacgtt aaaaaatccg
    36721 agaagaatct aaagtacaca acattggcat ataataggaa cgtcaataag aagccgccta
    36781 tgaacatgaa ccaaaatgct tttttgcctt taaagaatag gaacacactt aaaaccagtg
    36841 ctactaaact ataaggattc atcaataata ttaagttttg tactaaacct ttaacaccta
    36901 aagaaaaatc aacataataa gaaaaatacg tcttcaaggt aatcgttatt acggttaata
    36961 agaagaacgc aaaaagacta attttctttt tttgtgaact catgattctt tcccccgtta
    37021 tttagataat aaatcttgcg aaagtacttg aataaattag atattaacat tatgtagtta
    37081 attaataagt gcgtttaaaa gttaacattg tcataattat agtaattact ttcagattca
    37141 agtttataat tttcattatt tgtagctttc aactttgatt taacaagtat tttgggctac
    37201 aactcatatt tgcttgtgta agtttcttta tttaatatta tcattcaatt aacaaattta
    37261 aaatttgtaa tcaaattgta ataataagca tagctcaatt aataaatata gagtaatttt
    37321 agtcaaaatt caagtaaaaa cacacttcat cttttcattt tactgtgaaa cgaattagaa
    37381 tatgatacat ttcgcagata aaacacagta ttatttcatt atacattagg aggaaattat
    37441 ttgcatcgga ctcgagtatg agctacgtac caaatattat ttcatcattt ctaattgcat
    37501 tctatattct attccatgga ataattggtt taaccagtta ctatacttaa caaggttctt
    37561 ttcagccgtt aaaatatcta taaattgaaa tttcaaacgt gatccttgcg gtttttgtgc
    37621 taatttcgtt aaatgataac ttgcgattgt accgatttgc gggtagctac ctagcgtata
    37681 atgatcattt aataagataa taggtgtgcc atcacgctta atttgtatcg ttcctttttt
    37741 gacagtctga tacggtggca tatcttcata aaaggctttg attttttcac cttcaagcat
    37801 catccccatg cgatttgctt tgctcgttac tttataatca tgtttcacaa atctttgaat
    37861 ggcatcttct ttaaaatctt ccgtaccttt atttttaacg acatgaaata catcagacat
    37921 ataattaaat gacaaggcgt atccatcaat accccaatct gtttgtttcg tgtgagcaag
    37981 gttttcaaat aacttatgat gacgagctgt ataatctctc ttaagcttta tttcatcgcc
    38041 atcttgtaat gttctacctt taaaaccacc aatttttaca ttaaagtcgg ttgagttaga
    38101 tcctaaccat gcatctaatt caaatccgcc tcccacagct aaatacactc gcgatgtata
    38161 acttgtttct ctaaacttta aaacatcgcc tttatctaac aaatacaatt tatatggaga
    38221 tatagtcata tgctcagtgt aagctttgac attaccccct gctaatgcaa tcagcgtagg
    38281 ttctgtaaaa cgaatcgttg ccattttatt agtcatttcc aaagttgctt cattcttgtc
    38341 atttgcaact aatcgattag caatttcatg tgctaaagta tcaagtgcac cacatggaat
    38401 tacaccatca tgttcatatc cccttctgcc aaagtcctga aagctactga ataagccact
    38461 tttttcaatt atgattgaca tctttaaacg ctcctacttc taattcattt tcattgatag
    38521 gtctaaattt aatattatcg cctaacttta agcgtgcgaa atctgattct ttcggattaa
    38581 aaagtgataa tggtgtataa ccaataacta accaatcatt aattgtatcc gtagttacaa
    38641 taccgcattt tttcccttca agtactacag aaccagctgg aatgaatttt ttctgtttac
    38701 tcgtgtgatt aatatacaat ttcttattta atccagttaa ataaggaaac ccaggtgaat
    38761 atcccatcat cgaaacaaaa tattgcgcct tagaatgtaa ttcaataaaa gtttctagct
    38821 tgattttgta atgttttaaa agtgattcta aatccggacc atatttcgaa ccatacacaa
    38881 ttggtatttc aatcaaatct tgctggttaa aatcatgttt tatgtttaaa tgaatcgatt
    38941 ctattagtgc tttcatgtat aaaaaaggtg attgtatatt atggtgttta atcatatccc
    39001 ttgcgtcata gacaatcatc atgtctgatt ctgatggcac aatttctata ataaatggat
    39061 aattttgttc aattaaatat gagcgaagtg ttaataaatc ttcggttaaa cttttagata
    39121 cttctttttc aattgcgact acaatggctt ggtcaccttg actatatatt ttcatttgta
    39181 cacctcttca atatttggtt actttacttt catttttaat tatagaatcg attgaatagt
    39241 tcattttacc tactcactaa atttactggt gatacattaa atccattgcc cgcacaacaa
    39301 aaggcatgag ttcgtgaatt aatgcgtata caaggataaa gcttataaca gtagtaattg
    39361 ttgctatcaa acgaacaaca tatattctat tttcagatag caatcgattc actaatcgat
    39421 aatttacgta tacaagaatt aacaacagca caatataaac aataatcata tttggcccca
    39481 tttaattttt aaatttgttt tcacatcatt ttctacatta ttatcataat aattttaaag
    39541 gcaaaggtgg acatcggcaa cctctcgtaa actatttatc aaaaataaac gtatctcatt
    39601 gttatgatat ttataaatca attcgttttt attaaagtct ttttcaacaa gtttgtcact
    39661 atctattaaa taatcacgca tgcatccttt taagaaatca tctttataac ttggtgtgta
    39721 ccattttcca tcctcttcaa tgacaatgtt gccaatatca aattcaagga ccttgccgtc
    39781 ctctgaagtt agcaatatta aatctgtttc atgattgtga gctaaatgct ttcgttctgt
    39841 cgttttatta tttataaaag ttggattaac tactttgggc aacacttgaa attttgctgt
    39901 aaaactactt ttctcaggta aaggtgacac tatcgtttca aattcgcttt cagcatttaa
    39961 tacgatttta agtctatatt ggccactgtg atactttgtt gttacatcgt ttaattcatt
    40021 tcgccatgca tgttcatcaa atttaaagtt taatcgctca gaagaacatt ttatgcgatt
    40081 agtatgataa gtaagtctag ggatatgtcc attttcaatt ttcattgttt caaataattg
    40141 cattataaca tctccaaaat cttggttttt gcgtaaaatt cattcacttc atcttttggc
    40201 ttagaatcaa ttgtaatacc tgctccgact ccataaatcg cttgtccata tttatactca
    40261 atagtacgaa tcggaatatt aaaaatcatt ttatcatctt cagttggaag taatagtcca
    40321 attgctccgc agtatatacc acgaggtgaa ctttctaatt gtttaatata tttcattgta
    40381 ttcagtttcg gtgcacctgt aatcgaacca caaggaaata acgatgttaa gatttgagtt
    40441 aggtctgtat tagtttttaa agtaccactt accatcgaag tcatttgaaa tacagtttta
    40501 tatgcctcaa taaaaaatag tttatacacc ttaattgagc cactctgtga tattctccct
    40561 atatcgtttc ttagtaaatc aacaatcatg acattttctg cacgatcttt cgaagaagtt
    40621 tgcaatgttt tataatactg ttgatcttca gcttccgttt tacctctagg cattgtccct
    40681 ttcatcggtt tgcttatgat aacgttatcg acattgttaa actgtccttt ttgaaaaaat
    40741 aattctggtg agatagacgc tacttggatt tcatctgttt gtaataacgc agtataatta
    40801 ccattactaa attgagttaa tcgttcatat aaagtactaa taggataata aatgttatcc
    40861 gttaagcgcg ccgtatagtt cacttgatac gtttcgcctt caacaattgc ttgttggaca
    40921 cgtttaatat tagtcatcat aacctcagaa gattcaacaa atgaaaaatg atgctttgat
    40981 acataagaag tttgatgttc atatgttgaa tttatgcttt ccgctttttc aaaactataa
    41041 gctactgcat aaatatcatc tttagctaat gaatgtgtac acataacatg attaaaatac
    41101 tttgccgctt cgtaacttaa atataacgag acatatctac cttgtcgttg ctgcgcttgt
    41161 gcaaagtgta tcacttctcc cacatcagcc aacttagtag caacatactt ctttataaat
    41221 ccctttaatt gaatatggta ttgcttatat tcattttcag ttaaatagta gcgataatta
    41281 tattctattc tcacagtaat cacctacctt cgataaaaat aattcaactt gtcgataacc
    41341 gtattcactc aaaatagatt caggatgata ttgcacacca aaaaccggaa atgtaatatg
    41401 ctcaaatgcc ataataatcg cttcatcgtt tttggctgta atctttaagc aatttggaaa
    41461 agtcgctccg tcagcaatta atgaatgata acgcattaca ttgaaatttt gaggcagtcc
    41521 ttgaaaaata ccttcattgg tatggcgtaa ctgtgtagta tgtccgtgta caggatgata
    41581 gccgtgaatg atatttccac caaaataaga cacgatacat tgaaatccta agcatacacc
    41641 tagtataggt acacgctgat aaaattgttc taacacttca ttcaatatag gataatcatc
    41701 cggattaccc ggcccaggtg aaataacaat tgcttttggc ttcatattaa tgacgtcttc
    41761 tatcagcaga ttatcaatac caacaacttg aactgttagt ttcgtttgag tcttaatata
    41821 gtctattaaa ttatatgtaa atgaatcatt attatctatg actagaatca ttgtatactc
    41881 cgttctaaat gtgttttatt tttataatat gtattggatg tagctaaaac tttaaaagca
    41941 ttgtcattat cctgaccttg attttaacta atatatagta tattctattc atcgtacata
    42001 aatgaatatc agaggttcct agctgaaacc ctctataaaa aactagacat tgaaatttca
    42061 aacattcgtt ggtaaacctt taatatatat tataggagca accc
//
LOCUS       DBNENW010000004        41008 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNENW010000004 DBNENW010000000
VERSION     DBNENW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41008
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.004"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            203..406
                     /locus_tag="AC1LW8_000170"
     CDS             203..406
                     /locus_tag="AC1LW8_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772040.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            589..1524
                     /locus_tag="AC1LW8_000171"
     CDS             589..1524
                     /locus_tag="AC1LW8_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIP4772041.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(1605..2573)
                     /locus_tag="AC1LW8_000172"
     CDS             complement(1605..2573)
                     /locus_tag="AC1LW8_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIP4772042.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2706..3050)
                     /locus_tag="AC1LW8_000173"
     CDS             complement(2706..3050)
                     /locus_tag="AC1LW8_000173"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP4772043.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(3092..3502)
                     /locus_tag="AC1LW8_000174"
     CDS             complement(3092..3502)
                     /locus_tag="AC1LW8_000174"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIP4772044.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3867..5933)
                     /gene="ptsG"
                     /locus_tag="AC1LW8_000175"
     CDS             complement(3867..5933)
                     /gene="ptsG"
                     /locus_tag="AC1LW8_000175"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0055056 - D-glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - D-glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HIP4772045.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6234..7973)
                     /locus_tag="AC1LW8_000176"
     CDS             complement(6234..7973)
                     /locus_tag="AC1LW8_000176"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HIP4772046.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELRAFVEAAKIPVVHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(8020..8709)
                     /locus_tag="AC1LW8_000177"
     CDS             complement(8020..8709)
                     /locus_tag="AC1LW8_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIP4772047.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8702..9097)
                     /gene="cidA"
                     /locus_tag="AC1LW8_000178"
     CDS             complement(8702..9097)
                     /gene="cidA"
                     /locus_tag="AC1LW8_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HIP4772048.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALFSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9310..10188)
                     /gene="cidR"
                     /locus_tag="AC1LW8_000179"
     CDS             complement(9310..10188)
                     /gene="cidR"
                     /locus_tag="AC1LW8_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cidABC operon transcriptional activator CidR"
                     /protein_id="HIP4772049.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10355..10573
                     /locus_tag="AC1LW8_000180"
     CDS             10355..10573
                     /locus_tag="AC1LW8_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HIP4772050.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            10963..11394
                     /locus_tag="AC1LW8_000181"
     CDS             10963..11394
                     /locus_tag="AC1LW8_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4772051.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(11532..12812)
                     /locus_tag="AC1LW8_000182"
     CDS             complement(11532..12812)
                     /locus_tag="AC1LW8_000182"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIP4772052.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAERILQDLRSQQ"
     gene            13066..14232
                     /locus_tag="AC1LW8_000183"
     CDS             13066..14232
                     /locus_tag="AC1LW8_000183"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIP4772053.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(14402..14923)
                     /locus_tag="AC1LW8_000184"
     CDS             complement(14402..14923)
                     /locus_tag="AC1LW8_000184"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIP4772054.1"
                     /translation="MEYKSYYDSPVGRLELVSDGGSLTAVLFENQQGDSTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            15332..17437
                     /gene="clpL"
                     /locus_tag="AC1LW8_000185"
     CDS             15332..17437
                     /gene="clpL"
                     /locus_tag="AC1LW8_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HIP4772055.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(17506..17676)
                     /locus_tag="AC1LW8_000186"
     CDS             complement(17506..17676)
                     /locus_tag="AC1LW8_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIP4772056.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(17689..19683)
                     /gene="feoB"
                     /locus_tag="AC1LW8_000187"
     CDS             complement(17689..19683)
                     /gene="feoB"
                     /locus_tag="AC1LW8_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIP4772057.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVDKQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVVYPLVTAYVLTFIFYQIGHLFV"
     gene            complement(19695..19922)
                     /locus_tag="AC1LW8_000188"
     CDS             complement(19695..19922)
                     /locus_tag="AC1LW8_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIP4772058.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(20138..22627)
                     /gene="farE"
                     /locus_tag="AC1LW8_000189"
     CDS             complement(20138..22627)
                     /gene="farE"
                     /locus_tag="AC1LW8_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HIP4772059.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKKGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKIY
                     PQGATNVSKHQDVHGQDDVRSIVLDDKTMALYQEVKQQSASSFFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            22772..23320
                     /gene="farR"
                     /locus_tag="AC1LW8_000190"
     CDS             22772..23320
                     /gene="farR"
                     /locus_tag="AC1LW8_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HIP4772060.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLRDIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEEIDKIFHKVINIKIK"
     gene            complement(23396..23563)
                     /locus_tag="AC1LW8_000191"
     CDS             complement(23396..23563)
                     /locus_tag="AC1LW8_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772061.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKR"
     gene            complement(23675..25219)
                     /gene="pruA"
                     /locus_tag="AC1LW8_000192"
     CDS             complement(23675..25219)
                     /gene="pruA"
                     /locus_tag="AC1LW8_000192"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIP4772062.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(25409..26008)
                     /locus_tag="AC1LW8_000193"
     CDS             complement(25409..26008)
                     /locus_tag="AC1LW8_000193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIP4772063.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            26247..26438
                     /gene="cwrA"
                     /locus_tag="AC1LW8_000194"
     CDS             26247..26438
                     /gene="cwrA"
                     /locus_tag="AC1LW8_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HIP4772064.1"
                     /translation="MRILITGTVAILIILGLVKTIQDHEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            26678..29086
                     /locus_tag="AC1LW8_000195"
     CDS             26678..29086
                     /locus_tag="AC1LW8_000195"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIP4772065.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTYYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLKPR
                     RKDA"
     gene            29366..29572
                     /gene="copZ"
                     /locus_tag="AC1LW8_000196"
     CDS             29366..29572
                     /gene="copZ"
                     /locus_tag="AC1LW8_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HIP4772066.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(29662..30660)
                     /locus_tag="AC1LW8_000197"
     CDS             complement(29662..30660)
                     /locus_tag="AC1LW8_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HIP4772067.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(30683..31837)
                     /locus_tag="AC1LW8_000198"
     CDS             complement(30683..31837)
                     /locus_tag="AC1LW8_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4772068.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQQALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(32264..33772)
                     /locus_tag="AC1LW8_000199"
     CDS             complement(32264..33772)
                     /locus_tag="AC1LW8_000199"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIP4772069.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(33784..34647)
                     /gene="crtM"
                     /locus_tag="AC1LW8_000200"
     CDS             complement(33784..34647)
                     /gene="crtM"
                     /locus_tag="AC1LW8_000200"
                     /EC_number="2.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diapophytoene synthase CrtM"
                     /protein_id="HIP4772070.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(34684..35811)
                     /gene="crtQ"
                     /locus_tag="AC1LW8_000201"
     CDS             complement(34684..35811)
                     /gene="crtQ"
                     /locus_tag="AC1LW8_000201"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HIP4772071.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(35817..37310)
                     /locus_tag="AC1LW8_000202"
     CDS             complement(35817..37310)
                     /locus_tag="AC1LW8_000202"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIP4772072.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGIPMVTLSGQQVADKINAREAKNRK"
     gene            complement(37303..37791)
                     /gene="crtO"
                     /locus_tag="AC1LW8_000203"
     CDS             complement(37303..37791)
                     /gene="crtO"
                     /locus_tag="AC1LW8_000203"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HIP4772073.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(37966..38733)
                     /locus_tag="AC1LW8_000204"
     CDS             complement(37966..38733)
                     /locus_tag="AC1LW8_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4772074.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(39094..40905)
                     /locus_tag="AC1LW8_000205"
     CDS             complement(39094..40905)
                     /locus_tag="AC1LW8_000205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP4772075.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT        13065 a         7819 c         6291 g        13833 t
ORIGIN      
        1 ctatattcaa aaataactta ttacacatat atttgcaata agataattaa agtaggatat
       61 tatttttagt tttctgatag gaatgatgat agtctatagg ttgaatcttt acttttttta
      121 aagctaaatt tacatcaact taacaatggc tggttatact gaagatgaaa atatttagta
      181 taacttagtg gaggcgataa aggtgcaatt gagtcattcc gttaaagttg caatttctat
      241 ctatttagca cttatcttta taacgttcac ttcttattta gtcattattt tatatacgag
      301 tatgactgga catgatgtat cacatttcgt gttagatagt cagcattctc atcatggatc
      361 tcttacgcaa aaacatttga gtcttcctga aatctcattt aaatagttta tcctctgtgt
      421 ttcaacattc atttcccata tcgattcatt tatctatcat ctagaccact acatcttaga
      481 tgattttttt attttctcat ttcactcttt cttaaaagtc gatataatga attaaatcat
      541 tatcatacac cgacatattt tatgttgttg gtgttaagtt ttaaaggggt gagatacttg
      601 gcgaataatc attcagcttt gcgtttaaaa ggaattatac ttgccattgt cggtgcttgt
      661 ttatggggat taggtggtac tgtttctgat ttcttgttca aatataagaa tattaatgtc
      721 gattggtacg tcactgctcg acttgtagtc agtggtgttt tcttacttat tatgtacaaa
      781 atgatgcaac ccaaacgttc tatatttagc gtattccaag atcgacgtat gttaggcaaa
      841 ttacttatct tcagtatact gggcatgtta gttgtacaat atgcttatat ggcatctatt
      901 aatacaggta atgctgcgat tgcaacatta ctacaataca ttgcgccagt ttatattatt
      961 atttggtttg tcataagagg cgttgcaaaa ctaacattat ttgatgtgct tgctattatc
     1021 atgacactat taggaacatt tttattatta acgaatggtt cattttctaa tttagtcgtc
     1081 aatcctgcaa gtttattctg gggtatttta gctggtgtag cactcgcttt ttacacaatt
     1141 tatccttcag acctacttaa ccgcttcggt tcgattctaa ttgtcgggtg ggcaatgctt
     1201 atttctggtg ttgcgatgaa tttacgccat ccaatttggc acattgatat cactaaatgg
     1261 gacatatcaa ttatattatt tttaatcttt ggtattatcg gtggtaccgc actcgcattt
     1321 tatttcttta tcgacagttt acaatacata tcagcgaaag aaacaacatt attcggaact
     1381 gttgaacctg tcgtagccgt tatcgcaagc agtctatggt tacatgtggc attcaaacca
     1441 tttcaaatcg taggcatcat tcttattatg attttaattt tattactatc acttaaaaga
     1501 caacctgaag cattagatga ataagaaaac tctgataatc actttagcaa gtaactattt
     1561 ttaacaacgt agctaccttt tatgtgatat cagagttttt tattttaatt aataatattt
     1621 ttcacttggt ataaaaagcg tcggcgctct ggtatcggaa atactggata aatatggaat
     1681 tggtaatatc caggtataaa gtcatgttcg ataccttttg cactcaacaa ttgcgataag
     1741 ttcaaagcat ctgggtacag tacttctttt gtgccaacag ttaatgtaat acgacctaaa
     1801 ccatctaagt caccattaat tggtgatact ttgtagttat ctaaaggtgt gtcccctgcc
     1861 cattgttcag ctaagaacac actgccatcc acacctacca ttgggtcttt ctttaagtag
     1921 tcaggaattt caggatgctg catcgttgca tctaaaactg gtgaaattaa tacaatatgt
     1981 cctggttgca caatatgttt ttcttttaac aattgagcaa atgataaagc aatttgaccg
     2041 cccgcagagt cacccattac aacgatttgt ttagaatctg ctacttgatt caataaatca
     2101 tggtataact tttcaaaaag cacatacgtc gcttgataat cttgatgcgg aatcttcgga
     2161 tatactggca tgatgacttt agcattgagt gtttctgcaa gttcatcaat aaattcgaag
     2221 tgaattttaa gtgggtcttg gaaccatgca cctccatgtg cgtataatac aacacgttga
     2281 tgcttatctt gtttatcatt aacggtataa acggttgaac cgtagtgctt atccactgtt
     2341 actggcatct taaactgata attggtgccc tcatattcac ctgctgtctg tggcgccatt
     2401 ttttctaatg ctttcatcgc ttcttcttta tacataaaac gacgcttagc tctagataat
     2461 cgtatacctt gttctaacat ataacttttc acactgcgtt tttctttaat tctaatatgt
     2521 gcgtatgctg ctactaaaaa tccaaacgca agtgtagacc attttttacg catttgtcgt
     2581 cactcctctt tgatgatttc tactatagtt ctactgtacc atgctgattc ttattactac
     2641 caatatttac tgggaaataa gttatgatgt ctacataatc ttagcaatgc taatcatttg
     2701 cgatattaat gctcatcgac atcgttattt acactatcat tcatttgctt aagatgatgt
     2761 tcaaaacgtt gcatatcgat aaaacgccct tgacgatgca tttcttttcc attcataaaa
     2821 attaaatcca caggtacagt gaaaatattt aattctccag caatagcttc cacctgactt
     2881 tgattaatca cagctaatgg cacattggga tatgattgca tcaagtcttc aatttgtggt
     2941 aaaacggcat gacacacgct acactgatcg cgcatgacat gtacaactgc taacggatat
     3001 tgatgaataa attcacggaa tgcttcgaca ctatttaatt gcttaaccat ttcgaacacc
     3061 ttcttacttt ttatatttta aaatgaagtc attattttgt tgctaaaatg tcttgaaaca
     3121 cttgtggcac agaggttatc ttcttatcgc tcaatctgta aaaaccgcct gtctgtgtac
     3181 caatacaatg cacgccatgt gcggacacaa tttcaaactg aaacacccat ttcatcttgc
     3241 ttgctttaac taagtaaata cgacctactg gtttgtcatg tattgtgaca ggtactttat
     3301 actctgcttc cgttttcata agtataggtg aaatgccttg attgatcatt gttgagtaat
     3361 tcatgtgctg attaataaac gctgttctca aatcttcgaa ccatctaata taaacgatat
     3421 tactaacgac acccatcgca tcaatgtcgt aaccattcac ttcgatttct ttctctgcaa
     3481 ctaatagacc ttctgttgtc ataatgacaa atctccttat cttacaaata gatttaaaat
     3541 catgttaatt tatttttatt actttcatga taaaaataca agcaattgaa ctttattacg
     3601 gaaaatcaaa cttttcaatg acgttattaa tttgactagt cgtgaagatg catttttcta
     3661 aaatgtacaa aaagtacaat ccctactttc tatcccccaa actaacatct atgccattgc
     3721 tatacatcat tgcgcactaa aaaggcagta gcttcatttt aagctactgc cttttgtaac
     3781 gtattctctt tcaactcgct acttcgcata cgcactacga atgactaaaa gttcattcat
     3841 ttgatatgct gtttcatttt taatcattat ttgactgtca taattagctt acctggatca
     3901 gcatcttgta catcttcaat gacaagttct ttattttcaa gatttgtaat aatcattggt
     3961 gtaacgatgc ttggtgcgtg tgctttcaag tatgctaaat tcactttcat taatggttga
     4021 ccttgtgtta ctttttcatc aacgttaatc agactttcga atccttcacc attcagtttc
     4081 actgtatcga taccaatatg aataagtact tcgacgccac tttcagattc taatcctatc
     4141 gcatgtttcg tagggaagat tgttttcact gtaccatcaa acggtgctac aatttcacct
     4201 ttttcaggga taaagccaac accatcaccc atcattttac cagcgaatac ttgatcaggt
     4261 acttctgata atggaatgat ttgaccgata cctggtgcat agatgtctgt tgttccaagt
     4321 gcttctacgt gaactggttc atctgacatt tcttcagtca ctgtcgtttc acttggtttc
     4381 gtaatttcac cactcataat cttggccata tcatgtttaa tttgatctga ttttggacca
     4441 aagatagctt gcatattgtt tccaacttct aatacacctg atgcgcctaa agctttaata
     4501 cctgctacat ctacttttga tttatcaacc acttctacgc gtagacgtgt aatacatgca
     4561 tctaaatgtt taatgttttc ttttccaccc attgcatcta agacatcaaa tggtaatttt
     4621 gcgacactag agttacgaat ttcagtttct tcatcttcac gacctggtgt tttcaattta
     4681 aacttacgaa ttgcaaagtc gaataagaag taatacacga tagcatatac aataccgact
     4741 ggaataacta ataatgcgtg tgaacgatcc cagtttaata aaccatataa aatataatct
     4801 atgaaaccac ctgagaatgt cataccaatt ttaacgccta ataaatgcat tactaagaat
     4861 gatgtaccag ctaataatac gtgaattcca taaagtactg gtgctacaaa taagaatgaa
     4921 aattctaatg gctcagtgat accagttaaa aatgcagtta atcctgctga taacattaaa
     4981 ccacccacga cttttttacg ttctggtcgt gcatttttat aaatagcaaa tgccgccgct
     5041 ggtaaaccaa acatcataaa tggatattta ccagtagtaa atgcaccagc agtaaatggt
     5101 acgccatctt tcaattgtgc catccaaata cgttggtcac cacgaaccaa ttcacctgcg
     5161 tgatttgtat aacttccgaa ttcaaaccag aacggtgaat agaaaatatg atgtaaacca
     5221 aatggaatta atgagcgttc aataataccg aatataaacg ttgttaatgt taaattttta
     5281 tttaataaga aattcgataa actatttaat ccatcttgaa ttggtggcca cgcaaagctt
     5341 aaaagcacac ctgttgcgat tgctacgacc gatgtcacaa tcggtacaaa tcgtttacct
     5401 gcaaagaatc ctaaaaatgg tggtagtgta atattataaa atttgttgta acaccatgcg
     5461 gctaaagcac ccatgataat accaccaaac acacccgttt gtaacgttgg aatacctaat
     5521 actaaagcat gtgctggttc tttcgctgct tgacttaatt cttttgcccc tttggcatat
     5581 gagaaaatgt catcaattgt aatgtgcaac actttcccca ttgttgcatt cataattaag
     5641 taacctacta gcgctgctaa tgctgcaaca ccgtctcctc ctgctaatcc aagtgctgta
     5701 ccaactgcaa ataataatgg caagttatca aatacaactt gtcctgctgc ttccatgacc
     5761 gacgaaatca ttacaatgat atcgtttttt aaccatggtg caatttctac taattgttcg
     5821 ttgtgcattg cgttaccaaa cgctaataaa ataccagctg ctggtaaaat cgcaacaggt
     5881 aacattaatg ctttaccgat acgttgcaat tgtccaaaca atttcttaaa catataaaca
     5941 tcccctatct caatttccgc ttccatgtac aaaaaaagca ggcatgaaca aacaagacta
     6001 ttctcatagt gcggttgttt gctcatgcct gcattaccag taacacgcaa gtgatatcta
     6061 attcatagct attatacata tttgtaaaag cgttttcaac aactttttca caattttatc
     6121 atcaattcag tacgatcatt tgtgataaat aaacaaaacg gcaattccca ttttgttgaa
     6181 atagaaatta ccgtttttat agttcaattc aattgtgata acctttaaat cagttataag
     6241 aaacgttttg ctgccactga aattggtgga atttgatcta aatctaaatg cttatcttca
     6301 gtaattgatc taaatgccca cttaccataa ccaagcgctt cttcatttac aattttacct
     6361 ggtaatggcg cagcattagg atcaacatat acatctacaa tcgttggtac atcttgtgct
     6421 aatgcctctt cgactatagc atctacttcg ctagcactct taattgtata acctttacca
     6481 cctgctgcct cagcaaattt tgcatgatcc atatcagaaa aatcaactgc atattctaat
     6541 tcaccagctg cttgttgttc atatttaata aatgctaact gtttgttatt aagtacaaat
     6601 acagttaaag gtaaatcata ttgtactgct gtagcgaagt cttgcattac catttggaat
     6661 gcaccgtcac cagcaatggc aatcgcttgt ctatttggat atgcaatttt tgatgccata
     6721 gctcctggaa gaccgcaacc cattgtacct aaccaacttg aaatgatgaa cttgttgttc
     6781 acaccaagat ttaaataacg tgtagaccaa actgttgcag taccaacatc tgctgaaatt
     6841 actgcatcat ctttaataaa tttgttaatt gatgccatta aacgttctgg acgtaatggt
     6901 ttgctattat tatttttatc ttgttccatc catttatccc aaaccgcttt acgttctaac
     6961 gttttgttta aaaatggtct ttccgcaaca tgtttaatat tttcagttaa ctgatgcaat
     7021 gcaattttac tgtctccaac aattcctaca ttaatattaa aacgatgtcc gatattttta
     7081 ggatttgtgt cgatttggat tgctttaata tttttcttag gtaagtaatc tacatatgga
     7141 tagtttgtac ctaccataat tagtaaatct gcatcctgca ttgtctgata tgaggttttt
     7201 gtacctattt tacctaagtt accaatactg tatggatggt catccggtaa gattgtctta
     7261 gcaggtaatg aatgaacgac cggaattttt gccgcttcaa caaacgcacg tagctcatct
     7321 ttcgcatgtt ttgcaccaac accaatcaac ataaccggct ttttactctt attgattaat
     7381 ttaaccgctt ttttaatgtc tttatatttt ggtgatacaa cagttggtct tgatgtatct
     7441 actggtttat tcgttgtatc tttaattttt tcagttaata agtcgttagg acaaataaca
     7501 acagctacac ctttttgttc atatgccgta cgaattgctt cgttaacaat ttcaaacaca
     7561 ttgtcacctt tttcaatttg gtgattataa acggctacat cttcacataa tttttgtaaa
     7621 tttgtttctt ggaatgcttt cgttccaagt gctgtactat tcgtttgtcc agataatatt
     7681 aattgcggta cattatccat tttggcatca tacataccat ttaataaatg aattaaacca
     7741 gggccaccga tacttaatgc cacaccgatt ttaccagtta attttgtgta accagcagcc
     7801 gctaagcttg ctacttcttc atgacgtaca tgataaaatt taaattgatc tctcactgta
     7861 cgtaaactat cgactactgc gtcgattgag tctcctggaa taccatacaa gtgatctata
     7921 tcccatgctt gtaatgcttt aactaatgct tcatttgctt ttatttttgc cattattact
     7981 aatagcctcc ctttctgtct atcattttaa atatctaaat taatagaata ataaaattag
     8041 aacaggtatt aacactgaac taataactgc agttaaaatc atcccaattg aactaaatgc
     8101 accggattca atatccattt ctagtgcttt agctgtgcca aatgcatgtg acgcattccc
     8161 atacgttaaa cctttggcga tagaagattc aaatcttcca aatcttaata acatcgaacc
     8221 taaaatacta ccgattaaac ccgttgtgat aataaaaagt acggtcatcg tatctgtacc
     8281 acctagttca tgtgacactt cgatacctac tgcggctgtt atagatcggg gcaataacgt
     8341 tacaatgacg tctttagaat agccaaatgc tttaagtgtt aagaacacta acatgaaatt
     8401 cagcatgacg ccagttaata cacttgcaaa aatgatagag acattgtctt taattttctc
     8461 tctattttta taaagtgggt acgctaaaca tacgaccgtt gcgtttaaaa tatggttgat
     8521 ccaactgcca cctttcatat acccgttata actaattcca aagataagta agacaaaaat
     8581 aattcctaaa gatgcaatta atgctggatt caaaaatggg ttcggatatt tttgttgtaa
     8641 ccttttagcg aaataatata aaacgacagt caacaaaatc attaataagg cttgcacgta
     8701 atcattcata agcgtctaca cctttacgat gtttatgttt aacagacatt ttttcagcaa
     8761 tataacctga aaataatgca acgatacatg ttcctatgat aatgactgcg aaaaagagta
     8821 tataatttag cgtaatttcg gaagcaacat ccataatacc tacaactgac ggtatgaaga
     8881 aaaagaccat cgtctttaat aaaaagtttg caccgtcttc tacccaagtt agcggtacaa
     8941 tcttaaattg taatagtaaa taaaataaaa atagaccaac aatactgccg gctaagggaa
     9001 gatgaaaaat cttttgaatt tctgtgccaa tataagtaat cacaatgatg attcctagtt
     9061 gtagtagtag tttgattatt aattggactt tgtgcatggc gccatccctt tctaaatatg
     9121 tctaaattgt tacaataact attataaaga tggcgtacac aatataaaaa tactcttttt
     9181 ttatagtttg tattactatt ttctatggaa acgctctcta aatttcactt cattttcaca
     9241 aaaaaacact cattatttca atgacccacg taatgatgcg ccttaaataa tgagtgcttt
     9301 attaatatgt tagcctaaac ggtctttcaa aaattctatc catttcgttg tggcatgact
     9361 taattgttta tccttcttcc aaacaacacc taattcccaa tgtacatgag catcttcaat
     9421 gcgtagcagc ttcacatctc catttagttg ctctgaaatt gatgttggta aaatactaat
     9481 acctaattca ttcgtaacta aatcttctat aacatgccat tgtgaaattt gcgctacagt
     9541 attcggaaca aagccaacgt ttttagcatt ttcaataatt ttatcattca agtaaaagtc
     9601 tttattaaat aaaatgaagt cttcacctgc taaatcttcg agtttaacag tttcatattt
     9661 tgcgagtcga tgctctctgc tcacgataag tcgcaaatct tccttatcta gggtagtata
     9721 atcgaaaata tgatgatcga ctggcaaagt ggtcacgcct atatctactt catcattgat
     9781 aatttgctgt tcaattgttt taccgccatt ttctatcaaa ttatatgtaa catttggata
     9841 ttgttgatgg aatgcaccaa gaatattgat aagaatcttc atattcatga ctgccgacat
     9901 gcccatgttt atatgtcctg tttccagtcc attcaagcgt tccatttcag atggtaaata
     9961 atcatacagt gcaacaattt ctttactttt ctcataaaaa atttgacctg catcggtaag
    10021 aattaaatgt cttttacttc tatcaaataa gggcgtgccc atttcatttt caatatcttt
    10081 aattgcttta ctaattgtag gttgtgcaat atataatgat ttggaagcat tagtcatgcc
    10141 tccttgctta acgacttcaa taaaatattt catatgtttg atatccacta acatcatcct
    10201 aactatttca acacgtttat ttcattatac acaacttctt aaacattcaa taaatttaga
    10261 tttataaaat tgctttataa aaagttttat gtaaaataca cattctttta tggtattata
    10321 ttaatggctc aagtatatta atcgaggtga ggtattgagc ttttacgaat ttatgcaaag
    10381 ttttcttggt gatgacacac cattaggtga attggttgat tggattaatc aagatagcaa
    10441 tttccctaga gaagtgaaga gccatagtga aatattgtca tattttcgaa caaatccatg
    10501 tcctgaaagc atctcagtac ctgtaattaa acgggcacta tcagttttca accaattcac
    10561 aaatgtacaa tgatacatga atacatggag tcacatcatc tcatgttagc gctctgattg
    10621 cagggcgcta tttttaatgt tattgctaca tttagtatat tgtgattgct attttctttc
    10681 agttatagaa ccacgtacct tttcttttaa atcattttat attttcccac taatatccgc
    10741 tgttaatcaa tcctgacatc cttgtatcac tatgacaatt aattgttaaa tacatgaatt
    10801 tctacatttt atgaaaaaat ccatttttat tacaattcaa cactttatat gacaacttca
    10861 ttacagttac ttttattgtt gattgcttac attgttttct aaaaaaaatt tgttatcata
    10921 attaacgttg aataaagaaa aaaattaact tgggagataa aaatggaata taaaaagata
    10981 ctaattcgtt tattaattgc ttttgcagta cttttctcag cagatttcac ttatcaatcc
    11041 gtcgaacaaa cgcatcaatc gcatgccgca gttaattatt atagtaaaaa ccaatgtaca
    11101 tggtgggcat ttaaacgtcg cgcacaagtc ggtaaacctg tttctaatag atggggcaat
    11161 gctaaaaatt ggtattacaa tgcacgtaaa tcaaaatatg cgactggtcg tacaccaaga
    11221 aaatttgctg tcatgcaatc aactgcagga tattatggac atgtcgcagt tgttgaacaa
    11281 gtatataaaa acggtagtat taaagtttca gaatacaact tttatcgccc attaaaatac
    11341 aatacacgtg tactaagcaa aaaggcagca cgtaacttta actatattta ctaatcaaaa
    11401 aacttctatc acgaacgctt caatttcctg tatgcgtgtg atagaagttt ttatttttgt
    11461 tcatattaat ttatctaagc gctacgatgg actgacctaa acttttcttt aaaatcgcta
    11521 tttgcaattt actattgttg gcttcttaaa tcttgtaaaa tacgttccgc tgcttgtgta
    11581 tttgcacggg gttctttttt caaagcttca gctactttag caatttcatc accttttgct
    11641 cctacaacaa tagctaatga tttatattgt aaactcatat gaccttgttg aataccttct
    11701 gacacaagcg cgcgacatgc tgcaaagttt tgcgctaaac caacggcagc aactacatga
    11761 cctaattctt gtgctgactc tacatttagt agctctaatg aagctttagc tattggtaat
    11821 actttcgtac cacccccaac aattgccaat gtcataggca cttcaattgt accaatcaat
    11881 cgttgacgat cttgatcgta acgccatgta gcaataccac gatactgtcc gtcacgactt
    11941 gcgtatgcat gcgcacttgc ttctgcacca cgcgtatcat ttcctgttgc taaaacaaca
    12001 gcatgtatgc cattcataac acctttatta tgtgttgctg cacgatgaat atctacttgt
    12061 gccaatacag aagcacgttc cattcgtttg gcaacctctt ctccagttct ctcgcccctt
    12121 gctaaatctt taacatcaat ttcgccttga actttaacaa cggacgctgt tgcatgattg
    12181 gataaaatac tcattaaaat gtcgctttgc ggaaattcat tttttaaaaa tgcagttatg
    12241 gcctctaaaa tcgtattaag catattagcg cccatagcat ctttcgtatc aacaaatact
    12301 tttaaagata gtaactgttg ctcaggaaat gtatcaatcg ctatacgttg gtaaccacca
    12361 ccacgcgctt taatagaagg atatgcctca tccgcaattt tatgaatttg cttttctaaa
    12421 gctttaatgt ctgctgataa tttttcagta tcgtcaacgc catcaaagac gatttgacct
    12481 atcataatac gttcagaaga taccgtttta aatccgccag tctgattcac tagctttgca
    12541 ccataactag ctgcagcgac aactgaaggc tcttccacca tcataggtac aacatatgcc
    12601 ttatcgtcca caatgatatt cggtaataat ccaacgggta atgcaccttg cgcgatgaca
    12661 ttttcaatta aactattggc tacttcttca tcgattaatg gatgattcag taaaatgtcg
    12721 aattgttctt ctgataacca ttgcttatca accaattgtt gtaacttttc tttacgagat
    12781 aaatgtcgaa aattcttatc taaattttgc atggacgtac tccttttact tcacataatt
    12841 tttaacattt caatcactac tatttttacc acaaaataac gtcattcgtc ttaaaattca
    12901 attgaataat tgtcgttttg actttaaaat aaaacaaggt aaattaaaac gcttacaaga
    12961 aacgacaaat catttttaaa tttagtatat ttctttgtat aaaattagca tattctgata
    13021 tgatacaagt gttgctttta taaatttgaa aggatgtaaa accttatgac aataggtatc
    13081 gataaaataa acttttacgt tccaaagtac tatgtagaca tggctaaatt agcagaagca
    13141 cgccaagtag atccaaacaa atttttaatt ggtattggtc aaactgaaat ggctgttagt
    13201 cctgtaaacc aagacatcgt ttcaatgggc gctaatgctg ctaaggacat tataacagac
    13261 gaagacaaaa agaaaattgg tatggtaatt gtggcaactg aatcagcagt tgatgctgct
    13321 aaagcagccg ctgttcaaat tcacaactta ttaggtattc aaccttttgc acgttgcttt
    13381 gaaatgaaag aagcttgtta tgctgcaaca ccagcaattc aattagctaa agattattta
    13441 gcaactagac cgaatgaaaa agtattagtt attgctacag atacagcacg ttatggattg
    13501 aattcaggcg gcgagccaac acaaggtgct ggcgcagttg cgatggttat tgcacataat
    13561 ccaagcattt tggcattaaa tgaagatgct gttgcttaca ctgaagacgt ttatgatttc
    13621 tggcgtccaa ctggacataa atatccatta gttgatggtg cattatctaa agatgcttat
    13681 atccgctcat tccaacaaag ctggaatgaa tacgcaaaac gtcaaggtaa gtcgctagct
    13741 gacttcgcat ctctatgctt ccatgttcca tttacaaaaa tgggtaaaaa ggcattagag
    13801 tcaatcattg ataacgctga tgaaacaact caagagcgtt tacgttcagg atatgaagat
    13861 gctgtagatt ataaccgtta tgtcggtaat atttatactg gatcattata tttaagccta
    13921 atatcattac ttgaaaatcg tgatttacaa gctggtgaaa caatcggttt attcagttat
    13981 ggctcaggtt cagttggtga attttatagt gcgacattag ttgaaggcta caaagatcat
    14041 ttagatcaag ctgcacataa agcattatta aataaccgta ctgaagtatc tgttgatgca
    14101 tatgaaacat tcttcaaacg ttttgatgac gttgaatttg acgaagaaca agatgctgtt
    14161 catgaagatc gtcatatttt ctacttatca aatattgaaa ataacgttcg cgaatatcac
    14221 agaccagagt aatctgtgta tttgaaagca tttaaatata acctaaaaac agcagtaaga
    14281 cagcttctca ttgaaatcgt cttactgctg ttctctattt ataacacttc gtattgaatg
    14341 aattagctaa gcctgtttga cacattattg aagttttcct aatgcctgga tcctttatac
    14401 gttacggctt cgtgctatgt tttggtacat aaagctttga catatcgata ttctccaact
    14461 ctaacagctt aattttatta ttaatcgttc caccgaaccc tgttaagcta cccgttttac
    14521 cgacaacacg atgacatggc acgataatag ataatggatt acttccgact gcacctccaa
    14581 ccgcttgggc tgacattttt ggcttgttaa gcagcttgcc tacttttttg gcaatagcac
    14641 catacgttgt tagagtccca taaggaacct gtcttaattc attccaaaca cactgttgaa
    14701 aatgactacc tgttggcttt aaaggtattg tgatttcagg attgtcacct ttaaaatacg
    14761 cgtctaacca ctgtgtcgcc tctctaaata tcgctaaaga cgtattttct tccctagtac
    14821 tatcaccttg ttgattttca aacaaaacag cggtcagact tcccccatca ctcacaagtt
    14881 ccaatcgtcc tacaggcgaa tcatagtaac tcttatactc cataaaaatg cccccttttt
    14941 ctaatcatac gccttaattg ttaatttaga aaatattcat gttcacattt tttaaaaata
    15001 aataagtcgt caattacgta tataaacacg taataccagc tatcactttg ctgcaatata
    15061 cagttacata tcttactaca cgcgctaacc tcttactttg taaaccaaat cttaaattaa
    15121 aatattgaaa atgcaatgaa tccttaatat tttattaaac ctataattac ttattaaaaa
    15181 taacacacaa tattcataaa gttttaaaaa tattctgttt aatcatctat tattagtgga
    15241 aaaatataag agcaattgtt tatagtttgc ataacgcttc aaaagtattt tcttttttgt
    15301 ttagttcaaa aaaatttaga ggtgatgtta tatgaataac ggttttttca atagcgactt
    15361 tgattcaatt ttccgaagaa tgatgcaaga tatgcaaggt tcaaatcaag tcggtaacaa
    15421 aaagtactat attaatggta aagaagtttc acctgaagaa ctagcgcaac tcacacaaca
    15481 aggtggcaat cactctgctg aacaaagtgc gcaagctttt caacaagcag cacaaagaca
    15541 acaagggcaa caaggtggca acggcaatta tttagaacaa attggtcgta accttacgca
    15601 agaagcacgt gacggtttat tagatccagt cattggtcgt gataaagaaa ttcaagaaac
    15661 tgctgaagtc ttaagtagac gaactaaaaa caatcctata ttagttggag aagctggtgt
    15721 tggtaaaact gcgattgttg aaggtttagc acaggcaatc gttgaaggaa atgtaccagc
    15781 agcaatcaaa gacaaagaaa ttatttctgt agatatttca tcattagaag ctggaacgca
    15841 atatcgtggt gcttttgaag aaaatattca aaaattaatc gaaggtgtta aatcttcaca
    15901 aaatgccgta ttattctttg atgaaatcca tcaaattatc ggttcaggtg ccacaggaag
    15961 tgattcaggt agcaaagggt tatctgatat tttgaaacct gcattaagtc gtggtgaaat
    16021 ttcaattatt ggtgcaacaa cacaagatga atatcgaaac aatattctta aagatgctgc
    16081 attaacgcgc agatttaatg aagtacttgt taatgaacca agcgctaaag atactgttga
    16141 aattttaaaa ggtattcgcg aaaaattcga agaacaccat caagtaaaat taccagatga
    16201 cgtattaaaa gcatgtgttg acttatcaat tcaatatatt ccacaacgat tattaccaga
    16261 taaagcaatc gatgtgttag atattacagc agcacattta tctgcgcaaa gtccagctgt
    16321 cgataaagtt gaaactgaaa aacgaatttc tgaattagaa aatgataaac gtaaagcggt
    16381 aagtgctgaa gaatataaaa aagctgacga cattcaaaat gaaatcaaat cattacaaga
    16441 taaattagaa aatagtaatg gtgaacatac tgctgttgct acagttcatg atatttcaga
    16501 tactattcaa cgattaactg gtattccagt ttctcaaatg gatgataacg atattgaacg
    16561 tttaaaaaat atttctaatc gtttaagaag taaaatcata ggtcaagatc aagctgtaga
    16621 aatggtttca cgtgcaattc gccgtaatcg tgctgggttt gatgacggca accgcccaat
    16681 tggcagtttc ctatttgttg gccctactgg tgttggtaaa acagagcttg ctaaacaatt
    16741 agcaattgat ctatttggta ataaagatgc actgattcga cttgatatga gtgaatatag
    16801 tgacacaaca gctgtttcaa aaatgattgg tacaactgct ggttatgtcg gttatgatga
    16861 caattcaaac acgttaactg aaaaagtacg ccgtaatcca tactcagtca ttctatttga
    16921 tgaaatcgaa aaagcaaatc cacaaatttt aacattgtta ttacaagtaa tggatgatgg
    16981 taatttgact gatggtcaag gtaatgtcat caactttaaa aatacaatta ttatttgtac
    17041 atcaaatgct ggctttggca atggcaatga cgctgaagaa aaagatatta tgcacgaaat
    17101 gaaaaaattc ttccgccctg aattccttaa ccgcttcaac ggcatcgttg aattcttaca
    17161 tttagataaa gatgcattgc aagatatcgt caacttatta ttagacgatg tacaagttac
    17221 attagataaa aaaggtatta cgatggacgt ttctcaagat gcgaaagatt ggttaattga
    17281 agaaggctat gatgaagaat taggtgcacg tccattaaga cgtattgttg aacagcaagt
    17341 acgtgacaaa attacagatt actatttaga tcatacagac gttaaacatg tggatataga
    17401 tgttgaggat aacgaattag tcgtaaaagg taaataacga cactttaaca tatcgcgcat
    17461 caaaaatgag catcaggtcg cccttgcctg tgctcatttt tttaattatt tccctggaaa
    17521 atgattcgct gtgtgctgtt ctgttccaca acaatcacga ttaatgtcac atgtaccaca
    17581 ttttccttgt ttcgaacgtt tgaaaaattt cactagtgta tacaaggcat atccgaaaat
    17641 tgctaaaaaa attaaaatgt taataatgac tgacacttta accactcctt aaacaaataa
    17701 atgtccgatt tgataaaaaa tgaatgttaa gacatatgca gtgactagag gatagacaac
    17761 tgcaagtgcc gtccatttcc atgaataagt ctctttacgg attgctgcca ctgtagaaac
    17821 acaaggaata tacaatagta taaatatcat aaatgcatac gcagatagcg gtgtgaattg
    17881 attttgaatc acattaacaa ggcctgcatc acctgatgaa tagataatcg ccatcgaact
    17941 tacgataact tcttttgcta aaaatcctgg cactaacgta gcacctgctt gccatgttcc
    18001 aaatccgagc ggttgcacta acataccaaa gaaactacca accatatgta aaaaactttg
    18061 attgatattc acattgatac catgtggtcc tacataactt aatagccaaa tgactactga
    18121 gccgccaaaa ataaatgtac ctgctttacg aacaaagccc ttagcctttt cccaagtact
    18181 acgccacaac gttttaatgg aaggcacacg gtatgttggc aattccacaa taaagattgc
    18241 attatcattt tttaaaatcg tcttagtaag tactgtactg actaaaaatg ccataataat
    18301 acctaaaaca tacaagctta atactactaa agattgattc tctttgaaaa agatacctac
    18361 gaacaacgca tacactggca gtctagcaga gcatgacatg aatggtgcaa ttaatatcgt
    18421 tgttaaacgc tctttttcat tttcaatact gcgcgcagcc ataatactcg gtacgttaca
    18481 accaaatccg ataatcatcg gtataaaaga cttcccgctt aaaccgaacg attccataat
    18541 acgatccatt aaaactgcaa tacgcgccat ataacctgaa tcttctaata aagatataaa
    18601 gaaaaatagc acaacaattt gtggtacaaa gactaatact gatcctacac cagcaataat
    18661 gccatctgta attaaatctt gtaaaaatgg tataacgcca agataattca taatcgtctt
    18721 cacactatct gtaaatgtac cacctataaa tgcatcgagt tgatccgaca aaggtgtgcc
    18781 aatccatgta aatgtagttt gaaagatcaa ccacataatc gctagaaaga taggcatccc
    18841 tatatattta tgtgttaata tcttgtctat tctagagctg aaatattgct tatcttcatc
    18901 tggatacgtt accacgtctt gcaataacgt ctcaatataa tgattgcgta tacgctccat
    18961 ctctcgacga acagatacag cctctacttg tttatcaact tgatcacgta aactcgacaa
    19021 tttatttaca acctctgaat taagttcgtt tgcaatttgc atgttattta ataagaattg
    19081 aatcgcaata aatctagctt gatatttatc atgagatgtc tctgtcatta ttatttgaca
    19141 catattttta attgtctctt caatcttctc accataattg attttaaaat gcggttgata
    19201 cccttcccct agatgcttta tttcgccaag taaatgttta gttcctttgc ctgtacgtgc
    19261 cacaactgga aaaataggtg tttttaactt tttcatcaat ttatgataat cgatttttat
    19321 cccgcgcttt gtagctacat caatcatatt taatccgatg tatattggtt gattaagttc
    19381 taacaattgt actgttaatt gcatattcct ttttagttga ctcgcgtcaa caatgttaat
    19441 gattcctgaa aatgaatcgt ttaataaata gtctgtcact acagtttcat ctttagaaat
    19501 cggcgataaa tcatatgtac ctggtaaatc aattaattgt cctacatttt cttttagctt
    19561 ccctactttt ttctctaccg ttacgccact ccagttgcct atatattcat acgaaccagt
    19621 taaagcgtta aacaaagatg ttttaccaac attagggttt cctaaaatac aataattttc
    19681 cattcgtccg gctcctattc ttctaatgca atagaacaag catcgcaatg cctaatactt
    19741 aactgttgtc cgtttacttc aataatacat ggccctttaa ataaacattt ttgtttaatc
    19801 gttatgatag cgtcatctgt taacccaaag gcacttagac gatacaacat attctcatta
    19861 gcaatatcca ttcgctttat tttataagcc ttattcattt caccattttt aatgtttaac
    19921 atactatttc gctctcctat tagaaataat aatcattatc acttaaaaat cataaccctt
    19981 aaaattgtag ctcgcaatac tttatttaaa taattttcat ttttcatgta aaatttgtga
    20041 cattgcaaaa atgttttgta taatcacttt catgcaaaaa acctccgctt caaatgtcat
    20101 tgatagacat ttaaagcgga ggcatgtaca tgttatttta tgatttattt cgtagatttt
    20161 tttctaacaa ttgattgagc ttttcaatat ttttattcaa ctgttctata ttcgttactt
    20221 gttgttttga atttagttga tgcttgtttt gataatctat taaagcatca tataagaaaa
    20281 atgaacttgc tgattgctgt ttcacttctt ggtacaatgc catagtttta tcatctagta
    20341 ctattgaacg tacatcatct tgcccatgta catcttgatg tttcgataca tttgttgcac
    20401 cttgtggata tattttaaag gttgtgtcgt atgtttcctt actatcttta gcttcagatt
    20461 cctgtgattc aaccgtttta tatttttcaa gtgcatgtcc ttcaatatca actcgtggaa
    20521 taatgcgatt caaccatgct ggtaaatacc acgaaccttt tccaaacaat ttcgttaatg
    20581 caggaattaa catcattctg actacgaagg catcaaagag tacaccaaac gctaatgcca
    20641 tacccattga tttaatcatg acatcttctt ggaatacaaa cgcaaagaag acactaaaca
    20701 tgattaatgc agctgctaca ataacaggac cgctttcttt caaccctact ttgatagaat
    20761 aatcattatc ccctgtttta ctatattctt catgaattcg cgacataagg aagacttcat
    20821 aatccatcgc taatccaaat aagataccta tagtaataac cggtaaaaat gctagcattg
    20881 gtcctgtcgt ttcaatacca aacagacctt tcataaaacc atcttgcatt actaatgttg
    20941 taaatcctag cgttgccatt aatgacaaga caaatcctaa aactgctttt aacggtatta
    21001 ggattgaacg gaagacaatc attaataaga aaaatgccaa tacaacaatt actgttgcaa
    21061 ataaaggtat cgcctcattt aactttttag acatatcaat attaatgaca ctttgtcccg
    21121 aaatctccgt tttgaaccca tatttatctt gtgcatcttt atgataatct cgtaaatcat
    21181 gcactaaatc atttgtactc tctgcattag gccctttctt aggtatcacg accatcaaag
    21241 cgtaatcatt atctttactc atttgtggcg gcgtaacgat atctacattt ttcttatctt
    21301 taatatcttt atatacagac tgtaaatctt gttgtaatcc ttgtggatca tcctttttat
    21361 ctttcacatt tatcaacatc ggaatttggc cattaaatcc ttcaccaaat ttatccgaga
    21421 ttatatcgta agcttttttc tgtgtagaat ctgctggttt aacaccgtca tctggaatac
    21481 caagtcgcat atgactaact ggtattgcag ctgctactaa tatgattaaa cctagtaata
    21541 ctgccgcaag tgcatttcct gtaataaatt tagaccaagg cgtatcaata tcttttttaa
    21601 aattagactg caatttgttc actttaatgc gtttatggaa aatactaatt aacgcaggta
    21661 ataaagtcag tgcactgaat acagcaaata taacactaat tgccgaagca aaccccatca
    21721 ctgctaagaa atctatgcca actagcgata aaccgcatac ggcaatcaca actgtcacac
    21781 cagcgaagat gactgcgcta cctgctgttc ctattgcaag accaatgcct ttaatgtaat
    21841 ctgtttcagt tttcataact tgtcgatatc tgaataaaat aaataatgca taatcgatac
    21901 caactgctag tccaatcatt acggctaatg tcagtgtgac atttggtata tcgaatgcat
    21961 aagttaacaa actgataatg ccaaccccag atgctagacc aatcaatgca cttataattg
    22021 gtaatcctgc agcaatgact gaaccaaatg taatcaataa cactacaaat gcaacaatga
    22081 taccaactag ttcagaatta ccgcctactt ctgtagatgt cattcctgta ccagttaact
    22141 ctgtttgtac attatgattg tcacgtaagt ctttcaaatg acttttaaca ttatctctag
    22201 agccatcttt taaagatgtt tgactaacgt catatgtgat atctgcaaat gcagttgttt
    22261 tatctttact aatttgctta ttttcataag gatctgatat tttatcaatg tgcttgtcat
    22321 cttttttaat atcatctaac gttttcttaa tatctttagt aatgttcggt tgcacaatac
    22381 catcatcttt agtcgtctta aagacaacac gtatttgtgc cttttcacta tcttgattaa
    22441 aatgtttttc aatcttttta ttcgtatcca acgactctaa tcctgtcatt ttaatatcat
    22501 tgtcaaattt cggtgcattt gtagcaagtg gtatcaatat tgcagctaca atcactatcc
    22561 atgcaatgac cgcggaccat ttatgttttg cgatgaatgt ccccatctta tataaaaatt
    22621 ttgccaaagt atattgcctc cttttaaaat caacgttata gtttaaatat acagtgtaga
    22681 ttattgttag attatagtat ctatccccga cctcttaaag aatcaattgg aaaattttgt
    22741 atattaaact acacacaaag gagaaatgta gatgaaagag actgatttac gagttataaa
    22801 gacaaaaaaa gcattgtcga gtagcttgct acaattgtta gaacagcaat tattccaaac
    22861 gattactgtc aatcaaattt gcgacaacgc actcgtacac cgcacaacat tttataaaca
    22921 tttttatgat aaatacgatc ttctagagta tttgttcaat caattgacta aagactattt
    22981 tgccagagac attagcgatc gccttaacca cccatttcaa acgattagcg atacaattaa
    23041 caataaagaa gatttgcgag atattgcaga atttcaagaa gaagacgctg aatttaataa
    23101 agtattaaaa aatgtctgca ttaaaattat gcataacgat atcaaaaata atagagaccg
    23161 tatcgatatt gacagcgaca tcccagataa tctcatattt tatatttatg actcgttgat
    23221 tgaaggtttt atgcattgga taaaagatga aaaaattgac tggcctgggg aagaaattga
    23281 taaaattttc cataaggtaa taaatattaa gattaaatag tagatgagaa actcatgagc
    23341 gttaccaaca ttcataataa aaacgatagt gtacacgtta atgaattcgt gtacactatc
    23401 gttttttatt tttatcatgc ttatcgctat taaaataact gatacacaac acataaacta
    23461 tgaagaaaaa aataaatccg ctatctaaat gactttgact cagttgttta aatgaccaaa
    23521 ttgctaatac aattcccatt attattgaaa taacgtatct cacattctta tacctataat
    23581 ccttttctaa aaatatggtt gctattactt aatttttaaa gttataaata aaaagagcca
    23641 accgcaatgg atggcccttg ttcattatga agcattagaa catttctgaa acaacctttt
    23701 gttctaagaa gtgtaataag tagtctggac tacctgtttt agcgtccgta cctgacattt
    23761 tgaaaccacc aaatggatgg tatccaacaa ctgctgaagt acagcctctg ttaaggtata
    23821 aattgcctac atcaaattcg tttaccgctt taatccaatg ctcgcgatta tttgtaatca
    23881 ctgcaccagt taaaccgtaa tctgtatcat ttgcaacctc aattgcttca tcaaaatcgt
    23941 taactttcac aaagccaaca actggaccaa aaatttcttc ttgcatgatt ctatctttag
    24001 atttaagtcc tgaaatgatt gttggttcta caaagtaacc ttttgaatca tcagtgccgc
    24061 caccttgttc taatttacct tcttctttac caatctcaat ataattttta atcttatcaa
    24121 attgtttttt attaataact gggcccatat acgtattgtc tacagtattg cccaacgtta
    24181 attcttttgt taatttgatt gatttctcta atacttcgtc ataaacgtct ttatgcacaa
    24241 ttgcacgtga acatgctgaa catttttgac cagaaaaacc aaatgctgac gttacaatag
    24301 cttctgctgc catatctgta tcaatatttt catcaactac aatggcatct ttaccaccca
    24361 tttcagcgat aacacgtttc aagaagtttt gaccttcttg aacaacggca ctacgttcat
    24421 aaattctagt acctgtcgca cgtgatcctg taaatgtaac gaaatgcgta tctttatgat
    24481 caactaagta atcaccaatt tctttcggat caccaggaac aaagttaact acgccttttg
    24541 gtaatcctgc ttcttctaaa atttccatta atttataagc gatataaggt gtatcctcag
    24601 caggtttcaa taacactgta ttacctgcca caactggtgc taaagttgta ccagccataa
    24661 tcgcaaacgg gaagttccac ggcggaattg taacacctgt accaattgat ttatagaaat
    24721 atttattgtg ttcaccttca cgatcaagta ctggcttacc ttgagccaag tccatcattg
    24781 aacgtgcata gtattcaata aaatcaatac cttcagctgc atcaccaact gcttcatccc
    24841 atggcttacc tgcttcataa accataattg ctgcaatttc cgcttttcga cgacgaataa
    24901 ttgccgaaac acgtaacata agctctgcac gatcatttgc tgaccatgtt ttccaagatt
    24961 tataagcttc gtttgctgct ttaaacgcat cttcaacatc ttgttttgtt gcctttgatg
    25021 catttgcaat cacttgtgat gtgtctgcag gattgattga tttaattttg tcatctttga
    25081 aaatcttctc tccattaatc actaatggta tgtcttgacc taattctttt tccacgtctt
    25141 tcaatgcttt cttaaacata tccacatttt cttggactga aaaatcgtaa ccaggttcat
    25201 ttttaaattc tactaccatg tacacttacc ccctataaat tttgaaagtg gtttaaccct
    25261 ttgatttaat gatataacat catttaaact cattttacta tgattaaggt tagttttgca
    25321 atcgctttca tttttatgct ttatcactta ttctcaagta ttttgaaatt gattggttac
    25381 tttttaaaat ttatatgggt cgcaactgct actttatcgt ttcgtcattt aatgtttcgg
    25441 atggtaggtc attatcaatt ttacgaacga ctttacaagg gtttccaacc gctaagctgt
    25501 gtggcggaat atctttagtg acaacactac cagcaccaat cacactgcct tctccaatcg
    25561 tcacccctgg taacacggct acatgaccgc caaaccaagt attactgcca atatgaatgg
    25621 gtccggcttt ttcaaaacct tcatttctat gatggaaatt aagtggatgt gtcgctgtgt
    25681 agaatccaca attaggtcct ataaaaacat tatcgccaat tgtaatctgt ccaccatcca
    25741 taaagtaaca attggtgttt acatagacat tttttcctag tttaacgttc caaccataat
    25801 ctgtatcaaa aggaatcgaa atacttacat tgtctgttgt tgtttgaaat aattgatcaa
    25861 ttaattcctt tcttttattt gtagcactcg gtcttgtatg atttaattca aagcaaatat
    25921 ctttcgctcg tgcacgttca ttgattaagt attgatcaaa gtttgcatcg taccattttt
    25981 ctgctaacat tttttctttt tcagtcatta cacctttcaa ctcctaataa cttatttact
    26041 tgtttaaaag ttaatcaaat aaacattcgc ctatgcaact aatacgctat aacattatga
    26101 aatcatgacc ttatcaccct tatctataca attctcgcat caaatactgc taaagtagta
    26161 gataaattca atactacaga cgcattcatt ttttaatcta ttaacgtaca atgtgagtaa
    26221 gagaaatata aaggagtatg atagcgatga gaatattaat tacaggcaca gttgctatct
    26281 taatcatttt aggtttggtc aaaacgatac aagatcacga aatgacaaac gacacgagtc
    26341 gtcagttgtc agacaacaaa gatgatgata aagtcatcca tcttaataat tttaaaaatt
    26401 tacatgcgaa agaatttaac ccatctgatt tcttttaagt cacctaagaa ttgcaaatcc
    26461 agaagtcatt taagttttac ctttcattca tacatccttt aatattaatt acgacttctt
    26521 ttatatagat gctaaataga gagattgttg tgcaatgttt gcacggcaat ctctcttttt
    26581 ctttttaaaa ttggtaaaag taaaacgcaa cgattgactt atatacctat agggggtaca
    26641 ttagacgtgt aacaatgaat cacagggagg caataatgtg gctaatacga aaaaaacaac
    26701 attagatatc actggtatga cttgtgccgc atgttcaaat cgtatcgaaa agaaactgaa
    26761 taaacttgat gacgttaatg cccaagtgaa tttaactaca gagaaagcaa ctgttgagta
    26821 taaccctgat caacatgatg tccaagaatt tattaatacg attcaacatt taggttacgg
    26881 tgtcgctgta gaaactgtcg aattagacat tacaggtatg acttgtgctg catgctcaag
    26941 ccgtattgaa aaagtgttaa ataaaatgga cggtgttcaa aatgcaacgg tcaatttaac
    27001 aacagagcaa gctaaagttg actattatcc tgaagaaaca gatgctgata aacttgtcac
    27061 tcgcattcaa aaattaggtt atgacgcgtc tattaaagat aacaataaag atcaaacgtc
    27121 acgcaaagct gaagcgctac aacataaatt gattaagctt atcatatcag cagtattatc
    27181 tttaccacta ttaatgttaa tgtttgtgca tcttttcaat atgcatatac cagcactatt
    27241 tacgaatcca tggttccaat ttattttagc tacacctgta caatttatta ttggatggca
    27301 attttatgta ggtgcttata aaaacttaag aaatggtggc gccaatatgg atgtacttgt
    27361 tgctgttggt acaagtgcag catattttta cagtatttat gaaatggttc gttggctaaa
    27421 tggctcaaca acgcaaccgc atttatactt tgaaacaagc gccgtactaa ttaccttaat
    27481 cttattcggt aagtatttag aagctagagc gaagtctcaa acaaccaatg cgcttggcga
    27541 attattaagt ttgcaagcta aagaagcacg cattttaaaa gatggtaatg aagtgatgat
    27601 tcctctaaat gaagtacatg ttggagatac acttatcgtt aaaccaggtg aaaagatacc
    27661 tgttgatggc aaaattatta aaggtatgac tgccatcgac gaatctatgt taacaggtga
    27721 atctatccct gttgagaaga atgttgatga tactgtaatt ggttcaacga tgaacaaaaa
    27781 cggtactatt actatgacag caacaaaagt tggcggggac actgcgttgg caaatattat
    27841 taaagttgtc gaagaagctc aaagttctaa agcgccgatt caacgattgg cagatattat
    27901 ttctggttat ttcgttccta tcgttgttgg tatcgcacta ttaacattta tcgtgtggat
    27961 tactttagtt acaccaggta catttgaacc tgcacttgtt gcgagtattt ccgttcttgt
    28021 cattgcttgt ccatgcgcat taggacttgc tacaccaact tctattatgg taggtactgg
    28081 tcgcgctgct gaaaatggta ttttatttaa aggtggcgag tttgttgaac gcacacatca
    28141 aattgatacc atcgttttag ataagacggg taccattaca aatggtcgtc cagtcgtgac
    28201 agattatcat ggtgacaatc aaacgctaca actacttgct actgctgaaa aagattctga
    28261 acacccattg gcagaagcca ttgtcaatta tgcaaaagaa aagcaattaa cattaactga
    28321 gacgacaaca tttaaagcag tacctggaca tggtattgaa gcaacgattg atcatcacca
    28381 tatattggtt ggtaaccgta aattaatggc tgacaatgat attagcttgc ctaagcatat
    28441 ttctgatgat ttaacatatt atgaacgaga tggtaaaact gctatgctca ttgctgttaa
    28501 ttattcatta actggtatca tcgcagtggc agatactgta aaagatcatg ccaaagatgc
    28561 aataaaacaa ttgcatgata tgggcattga agttgccatg ttaactggcg ataataaaaa
    28621 cactgctcaa gccattgcaa aacaagtagg catagatact gttattgcag atattttacc
    28681 agaagaaaaa gctgcacaaa ttgcgaaact acagcaacaa ggtaagaagg ttgcgatggt
    28741 tggtgacggt gtaaatgatg cacctgcatt agttaaagct gatatcggta tcgccattgg
    28801 tacaggtaca gaagttgcca ttgaagcagc tgatattact attcttggtg gcgacttgat
    28861 gcttattcct aaagccattt atgcaagtaa agcaaccatt cgtaatattc gtcaaaatct
    28921 attttgggca ttcggctata atattgccgg tatccctata gctgcattgg gcttacttgc
    28981 gccatgggtt gctggtgctg caatggcact aagttcagta agtgttgtca caaacgcact
    29041 tagattgaaa aagatgcgat taaaaccacg ccgtaaagat gcctagattc cttaataatg
    29101 aaggattcgt tggtgattct gagataggct agtgattggc tctataatgt cgcggtttac
    29161 agttggattt tcgctccaac tgcatatata gttacacttt tcgcttggcg aattagtgta
    29221 tcttacctaa tagctccgcc tattaggttc catcattatt ataaataata agtacactac
    29281 ggtttacagt tggatcttcg ctccaactgc ataagagcca ctaattatta taaataataa
    29341 gtggctctaa aaggaggtga atgctatgtc acaagaaatt ttaaatgttg aaggtatgag
    29401 ctgtggtcac tgcaaaagtg ctgttgaatc tgcattaaat aatattgacg gtgtcacttc
    29461 agctgacgtt aaccttgaaa atggtcaagt aagtgttcaa tatgatgaca gtaaagttgc
    29521 tgtatctcaa atgaaagacg caattgaaga tcaaggttac gatgtcgttt aattaggcaa
    29581 tattcaacgt catcaacacc aaattaaaaa atcgaactga tgagaatccc aacaatccaa
    29641 attatctcat cagttcgatt tttaatttac tcgtaaccta gtatctccag tctgcaatac
    29701 atctaatgtt gcatctaatg catcgacaat tagattttta actgcagctt cagtataaaa
    29761 cgcaatatgt ggtgttaata tgacatcttc cctgtcaatc aacgattcta acaatggatc
    29821 gttcagtgtt ttgccccttt gatcacttgg gaaaagtttg cgttcaaatt catacgtatc
    29881 aagtgctgca cctttaatca caccattgtc taatgcgtct aataacgcct tagtatctac
    29941 taaagaacct ctcgcacaat tgacaaatac tgcgcccttt ttaaaatgtt taaataattc
    30001 agcattaaat agataatgat tatatttcgt tgcaggtaca tgtaatgtca cgatatcagc
    30061 accttcaacc gcttcctcaa tcgtatcttt gtaatcgaca tacgttgcaa ttttagcatt
    30121 aggaaacggg tcgtatgcga ccacatcact ttgataacca ttggcaaata tatcggctac
    30181 tacacggcca attcgacctg taccaataac agctactttt aaatctttaa tggatttcga
    30241 taaaatagta ggttcccatc taaaatcatg ctcccgcact ttcgtttgaa tttgattaaa
    30301 atgacgaacc acattaatag cctggttcac agcaaactcc gcaattgaat tcggagagta
    30361 tgacggcaca tttgacacaa tcaagttata cttgtttgct aactccaaat catatgtatc
    30421 aaatccagca ctacgttgtg cgatttgttt aataccaagt tcatttaatc gtttataaac
    30481 atgctctgat aatggtattt gttgtgatag cgataagcca tcataaccag cgacaccttc
    30541 aacattgtca tcagttaatg cttctttagt aatatctacc tcaacatgat gtttctctgc
    30601 ccacgccttg atataaggca tatcttcatc acgtacactc atgattttaa tttttgtcat
    30661 tttaacatca cccttaactt tattattcat ataaatatgc tagttctgtt aatcttattg
    30721 cagcttcgtc taatttctgg tcatctaacg ccaatgaaat tctcacataa cgattaccat
    30781 tctctccaaa tggtttccct ggagcaacaa gtattgactt ctcttgcact aaaaattgct
    30841 caaattgctc gctgtcataa ccaggcggtg tttccaacca tacatatatg ccacctttag
    30901 catgaacaaa tggcaaatca gcttttgcaa gcatggcttc gaatcggtca cgacgtgttt
    30961 taaatacatt gctttgttct tctaaaaaat catcataatg attcaaagca tatattgcgg
    31021 catcttgtaa tgcaccaaac atcccagcat ttgtgtgcgt ttggtacttt ttcaaagctt
    31081 gaatcatatc tttattacca actgcaaaac cgactctaaa acctgacatg ttataacctt
    31141 tagacaagga ataaatttca atcgcaacat ctttcccatt ttccgaagca agtatactag
    31201 gatttttagc gtcgaaaccg aaagcaccat aagcaaaatc atgcacgatt ttagtgtctg
    31261 tatctttaaa tttagctatc gcttcatcaa aaacttcttt cgtagctgtc gatccagttg
    31321 gattatttgg atacgttaaa taaatgagtt ttgttttatc tattatttgt gaatcaactg
    31381 tggaccaatc tggcaaataa tgtggcggtt ctaaattaag cgggacaggc ttgccatcag
    31441 ctaaaagtac acctgctaaa taatccgtgt agcctggatc aggtagtaat acatagtctc
    31501 ctggattgat aacacatgtt ggtactgcca ctaatccatt ttttgtacca tataaaatgc
    31561 atacttcatc ttctttatct aacgtcacat tatattgtct ttgataaaaa tctacaatag
    31621 cttgcttgaa cgcttcttta ccatgaaaag caccatattt ttgattttca ggaatagtta
    31681 gtgcttgttg aaaatgatca ataatacctt gtggcgtagg cccatcaggg attccaactg
    31741 ccatattaat taatggcaat ggtccatgtt cgattttacg tcccatcgtt ttcccgaaat
    31801 aactatcagg gatatttgct aatttgttag agatcatcaa attcctcctc tatcattaaa
    31861 catagcctgg gcgactatca taatcctaac aacttgtatc actctcattt agatggttac
    31921 aatgacatcg ccattcaccg ttatgttcaa cagaacttat gacacacgtt gtattgaatg
    31981 aatttatttt cattttaggt aggtataata ttattgtcaa tattaggaat tttcagatta
    32041 atatacactc aatcattatg attcaactgc catgcatatc cgcatgcgca accagttaga
    32101 tatgcttata taaagtataa cgcccatcaa ggtacgtatt caaacgtgaa ccttaacagg
    32161 cgtcattcat tgttaaataa aacttcttaa gcacatactt atttcactat gccttttacg
    32221 ttccccttat acttttctca catctttctc ttagactact cccttacacg ccttgctcaa
    32281 tatctttaat catttcatct acagttattt tcgcactcgt taagacaata ggaacgcctg
    32341 cacctggatg cgtacttgca cctgcaaaat ataaatcttt ataatctcgc gatacatttt
    32401 gtggacgata ataattactt tgcgctaaag ttggcattaa accgaatgcc gaaccaaatt
    32461 tcgcatgata cgtttgctca aaatcatttg gcgtaaagat tgtttctgaa acaatatgcg
    32521 attttatatc ttcaaatact tcaatcgttg ctaatttacg ataaataatt tcctttattt
    32581 gttgcgtcaa agcttcatct gaccaatcga ttccgctacc tgttttaagt tccggcgtcg
    32641 gcattagcac ataaatacca gttttgcctt ctggcgcaag tgatttatca gcgaccgctg
    32701 gtacatacac ataaatagaa ggatcatatg ataaacgtcc ctcaaatatt tcttcaatat
    32761 tgcctctaaa gtcatctgaa aaaataacat tatgaagtct cacttgatct gtcacatcaa
    32821 tatctatacc gatatacatt aaaaatgctg aacaagagta atctaagtct gcaattttat
    32881 gtggtggata ctttttaata ggtgcaaaat ctggcattaa tgattccgca acactaggga
    32941 aatcagccgt acataaaatt ttatcaaatt ttcttatgtc accattcact tttatcgcat
    33001 cggcccgttt gaatttagga tcaataataa tttgctcaat ttcagcattt agttcaatat
    33061 taacgcctaa gtctttattt aattgcgcta gcccttgagc catgccatac ataccgcctt
    33121 taataaaatg cacaccaaac atcatttcaa tcataggaat aattgaatat agtgacgggc
    33181 ctcgttttgg atcaattcct atgtataacg tttgaaacgc taaaagcttt tgtatctttt
    33241 cgttatcaat ataatgttca attagctgat ctgcatgatt taacgttttt aacttagcac
    33301 cttgcacaag tgacgtcata ttataaaagt cactcggttt gcgatacgtt ctttctaaga
    33361 aatagcgacg tgcaatttca tattttttat aaacatccgt taaaaaggac ataaaaccat
    33421 gcgttgaacc aggttctata ctttctagca tttgctgtaa ttcagctaaa tctgtaggca
    33481 ccgttatacg atcatcgtgg tcaaaataca catcgtaaat ataacgtaat tgtctcaatt
    33541 caatataatc ttcataattt ttaccacacg ctgtaaaaac atctttataa acatctggca
    33601 tcatgacaat tgtgggaccc atatcaaatg taaagccgtc tttctttaat tgattcatac
    33661 gcccgcctac attattattt ttttcaaata tcgtcacttc atgaccttga gaagcaatac
    33721 gggctgccgc tgctaatcct gtgacacctg caccaattac tgcaatcttc attattcaac
    33781 cacctatatt ctatgatatt tactatttat ttcatgaaac aactttgcct ttttccgctt
    33841 atccacaaaa acacgttcat gtaatgtata gttagcctgt ctcacttcgt ccagtatttc
    33901 aatatatata cgtgctgcta attctatgat tggttgtgct tcaatactaa atactttgat
    33961 ttgatccata acatcttgaa aatctttttc tgcgatagct gcataatatt cccataagtc
    34021 aatataatga ttattaacac cattttggta cacttcagca atatcaactt catattgctt
    34081 taatcgttgc ttactaaaat atatccgttc attgtcaaaa tcttcaccga catctcttaa
    34141 tatattaatc aattgcaacg attcaccaag tcttcttgcg acatcgtatg tctgatgtgt
    34201 ttcatgatca cttaaaatcg gcgtcaatac ttcacctact gtaccagcaa caccataaca
    34261 atatccgaat aattcagcgt ccgtttcaaa cattgtaaaa tgttgatctt tatatacagt
    34321 atcaatgaga ttatagaaag attgaaaggc gatattttta tgttgtgcaa catgctgaag
    34381 tgccatcatt attctacgat cactttgaaa gtgatgatgt tcatatgggt atttttcaat
    34441 agattgtata tcttctttta tttgatttaa aaattgaata tcgccataaa catctatact
    34501 gtcatcaatt ttacgacaca cagcataaat tgcccaaacc gcttttcttt gatcttctgg
    34561 taacaagtca aaagcgtaag aaaagctttt tgaatgtttc ttcatgattt tatgacaata
    34621 tttaaaattc atatccatca ttgtcatact agtcctccta tattgaaatg ccattacctt
    34681 gatttattgt tctttagata tagaatattg acgacctttc cattcaacta caccataccg
    34741 atgcgtttgt ttccaagatt ggatgaaaat tttagtaaaa aacataaata acaatggatg
    34801 acataccatt aataaattag aaaattggcc gacccttcga tgcagataaa taaattgtgt
    34861 agtatagctc aagtaaagtg ctaccatttt tcttacagac atttggcgat attttaaact
    34921 aagacatagc cctaaaatac tcgctataga accaaacaac cacaacacaa ttgctgtcat
    34981 gatcttaggc tttgtgccac ctgccccagt tgacaaatgc tttgtccatc cttcttgtaa
    35041 tgactgaaat ccttcttgat acatgcgaaa tgcaacaaat ggaaaccctt cataaactgt
    35101 tacgggcaat gattgtgaag tatatgcact tcctaaagca aatccttcaa taatatgacg
    35161 gtttgcactt ttatgacctc cagttgcata ataatcttct ttatttgtta atgtcaccgg
    35221 tccaaatgca tgctggttag tccgaccgtc gtctaaggta gaaaatacat tcataccaac
    35281 gactgtcatt aaattaaata tcgctgaaaa cccttcgtag aaacgctttg ttatatgata
    35341 aggctgtacg cttaacaatc ctttttcacc ttgtaattga tactgattaa tcaacgtttc
    35401 aacagcatct ttccttaaga aagttacatc agcatctaca aaggcaatac gattcgtaca
    35461 tgcatgcgtc acaccttgat aacaagcatg tgatttccca taccatttgc cgtcagtatc
    35521 atcaacaaca tctactacag tcgcaccata tgaacgtgct acacgtgctg tttcatctgt
    35581 cgatccgtca ttcataacaa tgacatctac tggaacttgc tgttgtatta tcgaatgtag
    35641 taaatgacct attctttttt cttcgtttct agccggaata ataattgtta atgctttatg
    35701 attgaagttc agcgttttcg cctttagctg atgtctacga ttaaatatca atgcaccaca
    35761 cgccatagac atggtcacta ttactgttaa tattcgtgat aaccatttca tagatatcac
    35821 ttcctattct tcgcttctcg cgcgtttatt ttgtctgcga cttgttgccc acttaatgta
    35881 accattggta tgccaccacc aggatttact gatccaccta caaagtacaa gttttcaaaa
    35941 tactgacttt ctttaggaaa tttaaatcct ttgtttttct ttttatctgc tacaacacca
    36001 tatattgcac cacgattaga acgataattt ttttcaatat cctccggtgt ccaaacatct
    36061 tcataaataa tgtgttttct taaatcagta agtcccattt tttctaattt atccaaaatt
    36121 ttatccctaa acttcgcata atcttcagtt gtaaaaggct gatcttgaat atatggaata
    36181 tgtggtaaga ctttgatatt ttcataacct actggcgctt gtgtgtgatc agttttattc
    36241 gtatttacta gataaatggt cggatcatct ggcaatacct tttcatgaaa aacttgttga
    36301 taattgagat aagcattttc cgtaaaaaag aaattatgat gtgctaattg cgggtattgg
    36361 caagcaacac ctaaatgcat cacatatcct gagcttgccg gctcaaattc cctctctaat
    36421 ttgtttaatc gttgagtatc aaggtgaatt aaatatttat aagtaggtat gacttccata
    36481 tttgaaataa tataatctgc ctttacaaac tcacctgtat ctaatctgac acccgtcaca
    36541 cgtctttgat atgttttaat attgtccaca cgtgcacctg tatgaattgt gacaccttct
    36601 tcacgcgcta gcttttccaa ggcattggct aaatgatgga ttccaccttc tacataccaa
    36661 aggccttgct cttgttgcat atggaataac atagataata cagctggcgc atcgtatgat
    36721 gaagaaccta catatttgat aaaatagcct aacatttgtc gcaagtatgg attcgagata
    36781 cgcttgtcta tggcctgttg catagtatgt acataatcat aattaataag agcatttaat
    36841 ggcccatgaa atttaataat ttgagatagt gtatctaaac catagttaaa ataccctttt
    36901 tcagtaatac gatcgattcg tcttgtataa ttcaaataat tttgcagttc ctctatatcc
    36961 tgtttcgaca atatcgcatt atgctgacct gtttctttaa taccttcata caaatcgata
    37021 gtcgttccat ctggaaaaaa gctacgccat tgatgtggca atcgcttgat tgtaacgtag
    37081 tctgacattt gcttcttgct atattcgaat aatttttcaa aaatataagg catcgttaaa
    37141 atagatggac ctaaatcaaa gccaaagcca tctgattcat gacgattcac tttgcctcct
    37201 atatgattat tttgttcata taatgagacc gaatagccac tttgtgccat tcgaattgct
    37261 gcagaaatcc cacctaagcc accaccaata acgatgatat gtttagtcat gacgttcacc
    37321 tcttcgtttt aatatgcgta gtaactgcgt taatctcggt cgattatagc gttgcacaat
    37381 aatgattgga acattagcta tcattgcata gaaaatattt atatacttta ctaaacgagg
    37441 gcctttattg aatatgatga ctggaagtat cgatatccaa tgaatcaact ctgccctctt
    37501 tgtctctata atcatttttt caatttcatc agtattgatt gtcattaaat gacgctgatc
    37561 atatatattt tgattaagct gatgaccatc taaaatctta tgtttccatt ttcttacgta
    37621 aaaccatttg ttccaaaatt ttccatgctt ctcaaagtta aatgatttaa atattatgta
    37681 cttctgacga aaatacttat caggaattct tgtacctaaa tttgctatat ttagttgaac
    37741 aattaaccaa tacatactac aaaaaaatgc tgttttaata tattttttca tggttttcat
    37801 ctaaattgaa tcactctcaa tcatactgac atagtataaa ttatcacttt attacttagt
    37861 tccctaaaga tcagtgaata aaccgctctc atttatattt atataaaaca aaaacgccaa
    37921 tactctcatg ttagagaata ttgacgtcat ttgatttaca tttgattaat gaatgaaatt
    37981 atatgaacct gcttggtttg ctgaaattgt acgagacgta accacaccag caccatgtcc
    38041 atagttcatt tctgaaacac gaacagaacc gttgctgtta acgccttcaa cgtaagcaac
    38101 atgaccgtaa tagccttgtg ttgtttgcat gatagcacca acttttggtg tattgttcac
    38161 tgtatagcca gatgatgcag ctgcgttagc ccaattactt gcgttacccc atgttgaacc
    38221 aattttccca ccaacacgat caaatacata ataagtacat tgtcctgaag tatataagtt
    38281 acttcctgat gcataaccat tagaaattga acgaccattt gaagatggcg ctgcagttgt
    38341 agtcacatga acattattac ttgttgtgct ataacttgca cctgaaccac cagtataata
    38401 gttatttgtt gctgtttgac tattgtttgt atagttattg tattgatatg aatgattata
    38461 gtagttattg taagatgcat tgttataact ataagtattg tagttgttgt agtagtatgt
    38521 gttgttttgc gttaattgac ttggattcca atttcctgtc caagtgtaat gataattacc
    38581 ttgtgcatca attgtatacg tatagctgta tgattgagcg tcattagaat tatatccatt
    38641 gttattttgt tctgcggctt gtgcatcatg tcctgcaaat gcgatagtgg caagtcctgc
    38701 tgtagcgatt gtagctgtaa cgattttttt cataattaaa tatcctccca ataatcaaaa
    38761 cactctatac atatagtcat tagcgactca attattttaa attttatatt ttttattttt
    38821 ttagtatgca aataattata acttttatag ttttgcgaca aaacaaacta taacagagat
    38881 taggtgtctt gcgggctttg ttatcataat gtaatctgtt cctaataaag cttcattgaa
    38941 tgtaatatac caattagaat gtttacaaca ctttttctgc accgttacca tgggatttga
    39001 aaataaaaaa gcactaaact tttaatgaaa tacatcattt ttgataataa atatcagaag
    39061 taatgtaata tttcaaaagt ttagtgcatc aaattatttc ttatttgtag catgtgtttc
    39121 catcgttttt acaatcaaat cagtcagcgc tttactacct gcatattcta agtgaatacc
    39181 gtcatatgca aagtattccg gatgacctgc agaagcttta taccaatcga ctagatgtac
    39241 attagagcgc ttttcagctg cctcataaat taatttattt attctacctt cataatctct
    39301 aggtactcta atagaaacta aataaatgtc tgcttttcca aaactatcca atagttcatt
    39361 taattgatct ttcgtaaatg ccccatttgt accaagctct actacaactt tttgaccttt
    39421 tttagcatag tctttatatt gcgatttcac aattggtgta gcatcaacga gttgccgtcc
    39481 aactttacca tcaatttgtg catttggtat tttcttagta aagacattac caatatccac
    39541 catgaccgag tcaccaatta atagtggtga tgacttttta atatcctctt tgctattcgc
    39601 tgtctgctta tcctcttgct tatctttttt aacgactttt ttcttcgttg ttttaaattc
    39661 cgtcttcttc tctttcttct cggcttcatg ttgtttgcca agtgcatcaa actgtccact
    39721 gagcacaacg atagacggaa ccaataatag gataactaac actgttctag caaattgccc
    39781 cttcttttta ggtaaaaatg caaaagcttt aaatcctttt ttacgtatag gtgtttcaat
    39841 aaagcgatac gaaatttcag ccattaacgc tgttaacaaa atttctataa tataaacgta
    39901 taccggtatt tgtccttgta cgtaataact gttcacaaaa acaatgatag gataatgcca
    39961 taaatataag ctatatgatc gtttacctat aattagtaaa ggtttcatac ttaaaaattt
    40021 agcaaataaa ctagaaggat gtaccgcaat tgcaataatg aataaagttg caaatgatat
    40081 aatgtaaaat cctccattat agatccattg atcttggtcg ccaactataa agaacaaagt
    40141 cattagaacc gcaaaaccag atatccctat aatatctaat gatacgacaa tctttttaga
    40201 aatatctttt ttcaaagcaa acggaggcca aataaatgct aatatacaac caagcaataa
    40261 agtttgcagt cgtgtatctg tcccaaaata cacacgtgaa ttatctccag tgatgaaatg
    40321 aatcactatc ataagtccta aagaaatcaa cgatacaata aatagcgttt gaataatatt
    40381 tctcggttta aatctatgta ataagaacgt gataaccaat gggaaaagca agtaaaattg
    40441 ttcttcaatg gctaaagacc ataaatgttt gagtggttca atagcaaatt ggttaaaata
    40501 atctacattc tgtgagatgt accaccagtt tgaaacatag aatatagctg caatagcatc
    40561 tcgtttcatt tgtataatca attccggttt aaatatcagt gtgaacgtaa gcacgacaca
    40621 aattaaaaac aacactgccg gaatgagtcg tttcaatcgt cgcttccaaa actctagcaa
    40681 atcaattttt tgcgtccgat aatactcact tatcaacaaa cttgttatta aataacctga
    40741 aataacgaag aatgtatcta ctcctaaaaa gcccccactt aaccattgtg cattcaagtg
    40801 ataaataatg attcctataa ctgcgaatgc cctcaatcca tctaatccgg gtaagtatcg
    40861 cggggaatac attttttcta aacgtttaaa gtcttttgta tccatgttaa taaacgcccc
    40921 atttattttt ctttattttg tagcatatca caatattttt gaaaataaaa tattgcactg
    40981 attttcatta attgatttaa cccttaat
//
LOCUS       DBNENW010000005        39193 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNENW010000005 DBNENW010000000
VERSION     DBNENW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.005"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..348
                     /locus_tag="AC1LW8_000206"
     CDS             <1..348
                     /locus_tag="AC1LW8_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772076.1"
                     /translation="FLILCEFFYGIPFLGATFILSFGWQPLIFNALLYLILTIILLVN
                     RQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVLVVLSAPTYIPNKNA
                     RVIYTQYKDGHRE"
     gene            complement(539..1873)
                     /gene="stgP"
                     /locus_tag="AC1LW8_000207"
                     /gene_synonym="pbuG"
     CDS             complement(539..1873)
                     /gene="stgP"
                     /locus_tag="AC1LW8_000207"
                     /gene_synonym="pbuG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-thioguanine permease StgP"
                     /protein_id="HIP4772077.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(1986..4121)
                     /locus_tag="AC1LW8_000208"
     CDS             complement(1986..4121)
                     /locus_tag="AC1LW8_000208"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIP4772078.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            complement(4147..4242)
                     /locus_tag="AC1LW8_000209"
     CDS             complement(4147..4242)
                     /locus_tag="AC1LW8_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772079.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            4308..5195
                     /locus_tag="AC1LW8_000210"
     CDS             4308..5195
                     /locus_tag="AC1LW8_000210"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4772080.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            5376..6239
                     /locus_tag="AC1LW8_000211"
     CDS             5376..6239
                     /locus_tag="AC1LW8_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HIP4772081.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(6356..6487)
                     /locus_tag="AC1LW8_000212"
     CDS             complement(6356..6487)
                     /locus_tag="AC1LW8_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772082.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(6658..7566)
                     /locus_tag="AC1LW8_000213"
     CDS             complement(6658..7566)
                     /locus_tag="AC1LW8_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIP4772083.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            7699..7863
                     /locus_tag="AC1LW8_000214"
     CDS             7699..7863
                     /locus_tag="AC1LW8_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1832 family protein"
                     /protein_id="HIP4772084.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            8108..8425
                     /gene="mspA"
                     /locus_tag="AC1LW8_000215"
     CDS             8108..8425
                     /gene="mspA"
                     /locus_tag="AC1LW8_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HIP4772085.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(8609..11776)
                     /locus_tag="AC1LW8_000216"
     CDS             complement(8609..11776)
                     /locus_tag="AC1LW8_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIP4772086.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTFAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGNIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(11893..13158)
                     /gene="femX"
                     /locus_tag="AC1LW8_000217"
                     /gene_synonym="fmhB"
     CDS             complement(11893..13158)
                     /gene="femX"
                     /locus_tag="AC1LW8_000217"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HIP4772087.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            13547..13798
                     /locus_tag="AC1LW8_000218"
     CDS             13547..13798
                     /locus_tag="AC1LW8_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772088.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLAIIHMGILAHSTYVYLY"
     gene            complement(13790..14554)
                     /locus_tag="AC1LW8_000219"
     CDS             complement(13790..14554)
                     /locus_tag="AC1LW8_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4772089.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNNIEAVKN
                     KLQSERVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            14716..15156
                     /locus_tag="AC1LW8_000220"
     CDS             14716..15156
                     /locus_tag="AC1LW8_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4772090.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHKILLKETHND"
     gene            15149..16360
                     /locus_tag="AC1LW8_000221"
     CDS             15149..16360
                     /locus_tag="AC1LW8_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4772091.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYPIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(16462..16812)
                     /gene="sarV"
                     /locus_tag="AC1LW8_000222"
     CDS             complement(16462..16812)
                     /gene="sarV"
                     /locus_tag="AC1LW8_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HIP4772092.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            16938..17108
                     /locus_tag="AC1LW8_000223"
     CDS             16938..17108
                     /locus_tag="AC1LW8_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772093.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            complement(17390..18412)
                     /gene="moaA"
                     /locus_tag="AC1LW8_000224"
     CDS             complement(17390..18412)
                     /gene="moaA"
                     /locus_tag="AC1LW8_000224"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIP4772094.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(18432..19031)
                     /gene="mobA"
                     /locus_tag="AC1LW8_000225"
     CDS             complement(18432..19031)
                     /gene="mobA"
                     /locus_tag="AC1LW8_000225"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIP4772095.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            complement(19038..19271)
                     /gene="moaD"
                     /locus_tag="AC1LW8_000226"
     CDS             complement(19038..19271)
                     /gene="moaD"
                     /locus_tag="AC1LW8_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIP4772096.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            complement(19277..19723)
                     /locus_tag="AC1LW8_000227"
     CDS             complement(19277..19723)
                     /locus_tag="AC1LW8_000227"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIP4772097.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            complement(19737..20222)
                     /gene="mobB"
                     /locus_tag="AC1LW8_000228"
     CDS             complement(19737..20222)
                     /gene="mobB"
                     /locus_tag="AC1LW8_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIP4772098.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            complement(20219..21478)
                     /locus_tag="AC1LW8_000229"
     CDS             complement(20219..21478)
                     /locus_tag="AC1LW8_000229"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIP4772099.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            21541..22035
                     /gene="moaC"
                     /locus_tag="AC1LW8_000230"
     CDS             21541..22035
                     /gene="moaC"
                     /locus_tag="AC1LW8_000230"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIP4772100.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNKTIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            complement(22037..22543)
                     /locus_tag="AC1LW8_000231"
     CDS             complement(22037..22543)
                     /locus_tag="AC1LW8_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIP4772101.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(22574..23578)
                     /locus_tag="AC1LW8_000232"
     CDS             complement(22574..23578)
                     /locus_tag="AC1LW8_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIP4772102.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWDGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            complement(23748..24353)
                     /locus_tag="AC1LW8_000233"
     CDS             complement(23748..24353)
                     /locus_tag="AC1LW8_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4772103.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            complement(24354..25025)
                     /gene="modB"
                     /locus_tag="AC1LW8_000234"
     CDS             complement(24354..25025)
                     /gene="modB"
                     /locus_tag="AC1LW8_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIP4772104.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYHRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            complement(25039..25821)
                     /gene="modA"
                     /locus_tag="AC1LW8_000235"
     CDS             complement(25039..25821)
                     /gene="modA"
                     /locus_tag="AC1LW8_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772105.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTLVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            26111..26908
                     /gene="fdhD"
                     /locus_tag="AC1LW8_000236"
     CDS             26111..26908
                     /gene="fdhD"
                     /locus_tag="AC1LW8_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIP4772106.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            complement(27080..27850)
                     /locus_tag="AC1LW8_000237"
     CDS             complement(27080..27850)
                     /locus_tag="AC1LW8_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4772107.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIAEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(27927..28481)
                     /locus_tag="AC1LW8_000238"
     CDS             complement(27927..28481)
                     /locus_tag="AC1LW8_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIP4772108.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            complement(28602..29543)
                     /locus_tag="AC1LW8_000239"
     CDS             complement(28602..29543)
                     /locus_tag="AC1LW8_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIP4772109.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            complement(29941..30849)
                     /locus_tag="AC1LW8_000240"
     CDS             complement(29941..30849)
                     /locus_tag="AC1LW8_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4772110.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            complement(31063..32217)
                     /locus_tag="AC1LW8_000241"
     CDS             complement(31063..32217)
                     /locus_tag="AC1LW8_000241"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIP4772111.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELEGVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNSTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(32434..33339)
                     /gene="yut"
                     /locus_tag="AC1LW8_000242"
     CDS             complement(32434..33339)
                     /gene="yut"
                     /locus_tag="AC1LW8_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HIP4772112.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            33599..33901
                     /locus_tag="AC1LW8_000243"
     CDS             33599..33901
                     /locus_tag="AC1LW8_000243"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIP4772113.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            33915..34325
                     /locus_tag="AC1LW8_000244"
     CDS             33915..34325
                     /locus_tag="AC1LW8_000244"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIP4772114.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            34322..36037
                     /gene="ureC"
                     /locus_tag="AC1LW8_000245"
     CDS             34322..36037
                     /gene="ureC"
                     /locus_tag="AC1LW8_000245"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HIP4772115.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            36050..36502
                     /gene="ureE"
                     /locus_tag="AC1LW8_000246"
     CDS             36050..36502
                     /gene="ureE"
                     /locus_tag="AC1LW8_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HIP4772116.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            36495..37184
                     /locus_tag="AC1LW8_000247"
     CDS             36495..37184
                     /locus_tag="AC1LW8_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HIP4772117.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            37197..37811
                     /gene="ureG"
                     /locus_tag="AC1LW8_000248"
     CDS             37197..37811
                     /gene="ureG"
                     /locus_tag="AC1LW8_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIP4772118.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFIINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            37811..38647
                     /locus_tag="AC1LW8_000249"
     CDS             37811..38647
                     /locus_tag="AC1LW8_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HIP4772119.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGIIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(38836..39183)
                     /gene="sarR"
                     /locus_tag="AC1LW8_000250"
     CDS             complement(38836..39183)
                     /gene="sarR"
                     /locus_tag="AC1LW8_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HIP4772120.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT        12460 a         7172 c         5735 g        13826 t
ORIGIN      
        1 tttttaatac tttgtgagtt tttctatggc attccttttt taggtgcaac gtttatttta
       61 agttttggat ggcagccttt aatctttaat gcattacttt atctaattct tactattata
      121 ttattagtga atagacaaaa tgcgattaga cctatagcga ttattccaat atttggtata
      181 gtaggtagtt ttttagctat catccccttc ttaggaattt tgattcattg gattttattt
      241 ttcctaatga ttctatttgt cctagttgta ttatcagcgc caacctatat acctaataaa
      301 aatgctcgtg tcatttacac acaatacaaa gatggacata gagaataatt cttaaaacct
      361 ttttaatcag gtatataata tagaagaaac acgtccaaca attttaaaag aagtatattt
      421 atatcggata tttaaagaca ttcataatat tgctttatgt tatttcgctt cttattttat
      481 ccttacttca aagaaaaaac gactaccaat cattggcagt cgttttttgt gtctggattt
      541 aaccatgaac aaatatgaag tacagaatga atagtaccat taatacatac attatcggat
      601 gtacttcttt atgtttttta gagataagca ttgtaattgg atagaaaata aatccacacg
      661 caataccagt tgcaattgag tatgataaag gcatcataat gattgtaatg aatgctggca
      721 ctgcaacttc aaaacttttc cagtttatct cagcaaagtt tgcagccatt aatacaccta
      781 ccaccactaa agctggtgtc gtaactgcac ttgttacaac agcgattaac ggactaaaga
      841 acagcgctaa taggaaacaa caacctgtta cgatactagc aaatcctgtt ctagcaccta
      901 cagcaacacc tgaagttgat tcgatataag aagttgttgt agttgttcca aaaactgatc
      961 ctacaatcgt agctaaagaa tctgagaaca atgctctacc agctcttggt aatttattat
     1021 ctttcataat acctgcttga gtagcaacag ctactaatgt accagctgta tcaaagaaat
     1081 caataaataa gaacgttaaa ataacaatta agaactgtat cgttaataat tgacttgggt
     1141 ctttaaacgc ttcaaatgca gcaccgaacg ttggttcaat acttggaatc ttacctacaa
     1201 taccagatgg catttgaatc aatccagtga acataccaac gattgctgtg atgatcatac
     1261 cgataaagat agaacccggc aatttaattg catataaaat aactgtaata acgataccga
     1321 atatcgccaa aagtactggt ggatcagtta aatgtcccaa tgttactaat gtagattcat
     1381 ttttaactat aatacccgaa ctttgtaaac ctacaaatgt aatgaataat cctatacctg
     1441 ctgaaacagc catcttcatt tgataaggaa ttgcattaat aataacttcc ctaaaccctg
     1501 tgacggttaa tatcgcaaag aatatacctg agaataaaac gcctgttaaa ccaacttgcc
     1561 aaggaatacc catggttaac acaacagtaa atgcaaagaa tgcatttaat cccatacctg
     1621 gtgctaacgc aattggatat ttagctatta gccccatgaa tagcgagcct acaaatgctg
     1681 ctaatgcagt cgctacaaaa atggcacctt ggtccatttt catatcttct gatacgcctt
     1741 taacacctgc taaacttaaa acttgcgggt taactgctaa aatataggcc atagataaga
     1801 aagttgtgat accgcctaag atttctcttt taaagtttgt tccatattta tcgaactgga
     1861 aatagttttt cacgaatgga tgctcctttt tttaaaatac cttaatagtt taatatcatt
     1921 cttaataaaa aacaatacca aaagcgaact tttcgttttt ctgattcaac tttgttcgat
     1981 tttatctata aattcaagtt ctttaatgca tctttaaacg gattattgtc taaaccttca
     2041 tttttattca tatacttttt catttcttta cgagatactt taccagagga tttagacttc
     2101 atacgctgat ccatatgtgc ttgcgtttca gaatgtccac aaacacaacg atataccgct
     2161 tctttccctt taccaaacaa cgttaatttc tttttacagt ttggacatct tgcatttgtt
     2221 ttgcgctgta cattcttttt cgtcttacaa gatggatctt ggcacacaag catctgacca
     2281 tttttagttt taactttaat catgaattta ccacacgttg ggcattctgt ggttgttaaa
     2341 ttatcgtgtt tatatttacg atcactattt ttaatcccat ttacaacatc tttcgtaaaa
     2401 tctttcattt cattaatgaa tgttttcgcc tgatatttac cacgttcaat taaaagtaat
     2461 ttttcttccc attgtgcagt taaaagtggc gacgttaatt cttctggtgc taattctaat
     2521 atttgtttac cttttgacgt tactttaatt ttaccgtctc ttgattcaat ggcattcata
     2581 ttaaataatt tatcgataat gtcggccctt gttgcaactg tgccgatacc acctgtttgt
     2641 tttaaagttt gcgcatattt tttatccttc aattgaataa agttctgagg gttctccatc
     2701 gcttttaata acgaaccttc attaaaatat tctggaggtg ttgtttcatg ttctctaata
     2761 tttgtttttg aaatcttcac ttcatcgcct tctgaaaaag gctgttgcat ctctgtaata
     2821 gattcacctt gtctaataga tttaaaacct aaaacagttg ttacattctc tttcaaaaca
     2881 aatgtgtgcc ctgcaacctc taaagttaca gttatcgcgt catactcgtg cggaggcatt
     2941 aaagcttcta aaaaacgctc gacaatcata tcgtataact ttaattctct attacttaag
     3001 tctgacatga caggtctcac ttctgtagga ataattgcat ggtgatcaga tactttttga
     3061 ttattaaata tcgacatttt tgatgaaaat gttttagaca ttaatgggcg tgcttggtct
     3121 ttatatgttg ttgccatcgt cgcctgaata cgttctttca tagtatctac catatcagtt
     3181 gttaaatagt ttgaatctgt tcttggatag gttacgactt tatgtctctc atataagctt
     3241 tgaagtgtat tcaatgtttc tttaggtcca attttataac gtctatacat atcttgttgt
     3301 aaatctgtta aattgtacag tgattgcgga tacgacttct tatgtttagt agcaacagat
     3361 ttaatcttac catcgacatt tttcaaatta ttaaccatct gttctaaagt ttctttattg
     3421 gtatatcgct gatttgattc tagctgaaaa tcaaaccctt ttaccgttaa tgataatgta
     3481 aagtattgtt gtggtttgaa ctgattaatc tcttgttgtc gtgtatttac taattgaatc
     3541 gttggtgtct gaacacgtcc cagggatagc tgtgcatcat actttgttgt tagtgcacgc
     3601 gttgcattaa tcccaacaat ccaatctgcc tcacttctcg ctaacgctgc ataatacaaa
     3661 tcgttatatt gacgaccgtc ttttaaattt ttaaaacctt gttgaatagc ttttttagta
     3721 actgagctaa tccataatcg acggattggc tttttgttac caactttatc caaaatcaat
     3781 cttgcaacta gttcaccttc tcgtccagca tctgttgcaa taataatatc tttcacttta
     3841 ttatctaaaa ttaacgcttt tactgtttta aattgtttgc ttgttttacc aataacaaca
     3901 gttttcatat atttaggtat aattggaagg tcttctaatc gccattcctt taaattttta
     3961 tcgtattgtt caggtgtcgc atttgtcact agatgaccta acgcccacgt gacaatatat
     4021 tggttatttt caaagtaacc attacgcttc tgatttattt gtaaagcatc agcaatatct
     4081 cttgcgactg atggtttttc agctaatatt aaagatttca taaattatcc tttctcatac
     4141 gttcttttat ttcgaacgtg cttcatctat tccactaatc tttgatttaa attcaatgat
     4201 tgcaaatgat gtgttaaatg tattgtaaca tgttaatatc actattaact ttcatttcag
     4261 ttgaaatact atataataaa agtaacaaaa agtacggagg taatgacatg agcatagttc
     4321 agttatatga tattacacaa ataaaatcgt tcattgaaca ttcgaattat gaatcagcat
     4381 catacttata taaacttcct caacagtaca atgaaataga tgtattaata accgatgcga
     4441 ttgaatcacc tggtgtattt tcgattaaag aaaacgattc aatcaaagca atcatattgt
     4501 cttttgcata cgataaaaat aaattcaaag tcataggccc tttcgtggct gacaattatg
     4561 tattatctgt cgatacgttt gaaacgctat ttaaagcaat gacttcgaac caacctgacg
     4621 atgccgtctt taacttttct tttgaagaag gcattcaaca atacaaacca ttaatgaaag
     4681 ttattcaagc aagttataac ttcactgact attacataga agcccgtaca agattagaag
     4741 aagatatgca ccaaccaaat atcattcctt atcacaaagg gttttatcgt gctttcagca
     4801 aattacacac aactacattt aaatatcagg cacagtcacc acaagatatc attgatagtt
     4861 tagacgacca tcatcatttg tttttatttg ttagcgaagg tttacttaaa ggttatttat
     4921 accttgaaat tgattcacaa cagtcaatcg ccgagattaa atacttcagt tctcatgtag
     4981 attaccgttt gaaaggtatc gctttcgagt tgcttgcgta tgcattgcaa tatgcttttg
     5041 ataattttga tattagaaaa gtttatttta aaattcgtaa taaaaataat aaactcatcg
     5101 aacgatttaa tggtctaggt ttccatatca actatgagta cattaaattc aaattcgaat
     5161 cacgtaacgt aaaagatcaa acaatccctg aataaaacac caagcaaata ccctacagta
     5221 catcattagc atgtattgtg ggtttttcta ctttttgtaa atattgaaaa ttataagtag
     5281 ttgtttttta ctattagggc agaatgcttt acaataacat gcaagtgtca attaagggga
     5341 gcacttgcat aaatagtata ggagagtgag tagtcttgca atttcttgat ttcttaatcg
     5401 cacttttacc tgctttattc tggggaagtg tcgttcttat taatgtgttc gtcggcggtg
     5461 gaccttacaa ccaaattcgt ggtacgacgc taggcgcact cattgttgga ttaggtttac
     5521 ttattactgg tttcgcaaaa ttcaataatc ctactgtaat tattgtcggt cttatttctg
     5581 gtgcattatg ggcgtttgga caagcgaatc agcttaaatc tattagttta atcggtgtat
     5641 caaatactat gccagtttct acaggtatgc aattagttgg tacaacatta ttcagcgtta
     5701 tctttttagg tgaatggtct tcaatgactc aaattatctt tggtttaatc gccatgatat
     5761 tattagttac tggtgtagca cttacttcac ttaaagctaa aaatgaacgt caatcagata
     5821 atcctgaatt taaaaaagca atgggtattt taattgtatc tacagttgga tatgtaggtt
     5881 tcgttgtact tggtgacatc tttggtgttg gtggaactga tgcattgttc ttccaatctg
     5941 tcggtatggc aattggtggc tttatcctat ccatgaatca taaaacatca cttaaatcaa
     6001 cagcacttaa tctattgcca ggtgtgattt ggggaattgg taacttgttc atgttctatt
     6061 ctcaaccaaa agttggtgta gctacaagtt tctcattatc acagttactt gttatcgttt
     6121 caaccttagg cggtattttc attttaggag aaagaaaaga tcgtcgtcag atgacgggta
     6181 tttgggcagg tattattatt atcgtgatag ctgctataat tctaggtaat ttgaaataga
     6241 aagttaaata ctcatgtaac gtaaaaatgt aatcacttct gaaaataacc attcacttat
     6301 agaatgatta aaattaattt tcgggaattt tacgttgaat gttcctctat atgtcctagg
     6361 aaatacgtgg ctctaaaaac aaaacgcaat aacacatcat gacattaatc atgcgtttta
     6421 agactttaaa attagcgata cttttaaaat cttgatgata ttcatatatc aagtatgcgc
     6481 catacatatg aagtggatag ctgcataacg cactgcatta tcaacttgaa tgtatgagtt
     6541 gaacaactat gtcataaata aaagccccct tttcacaata tacatttaca tattgtggta
     6601 aagggggctc tcattttcta cgaatactaa aatggatttt attttcaaat gtgtaaacta
     6661 gacaaacact gcctgataca cgtacaaaat aatgatacta ataatgattg tcaaattggt
     6721 cgtcatacct ataaatggca gtgttcgata tttaaactga ataccataag aaataattgc
     6781 aacacctacc gggaacatcc aagtgaccaa caatgtcgtc ttaatcatat catctgatac
     6841 tggtaacaac acatatacta acaatcccgc aactaatgct aatccataat gcaaacataa
     6901 atatttaata gtagcaggta tatactttct ttccagagta aaattcaaca tgacacctag
     6961 caaaatcatt gataacggca tatttgcatg ggaaagtatg ctaaagaaat cgattgccac
     7021 atgtggtaaa tggatgtgac ttatattcaa tataaacatt acaatgtatg taacgagtgg
     7081 cactgattgt aataatttct tacctaaata tttaaaatcg aattgatcac taccttcact
     7141 aaagtagcta cctacaaagt aagtaattcc aaacatcaca aaggcaccac ctatatcagc
     7201 cataacaaaa taaataagtc ccgttttagg ccatatcact tcaattagtg gatatgcaaa
     7261 caatccaata ttcatagcac cagtcatcat acctactgat ccacgcatgt gattatcata
     7321 ttttctaaaa aaccagacag caatcatttt cgccacaata ccatatataa tcattaaaat
     7381 tggtaaaatg gagaatgaca attttaattc tgcactgttt aaattcacaa taactaaaga
     7441 tgggagtgtg acattaagaa ctaatgtagc aatgacttga ctatctgttg cttttataaa
     7501 attaatgcgc ttcaaaaagt aaccaagcgc aattaataaa ataatcatag taaattgttc
     7561 tgtcactgtt atcccttctt tcaataatct tcataattta taactttaac atactccaca
     7621 gatattttag aagtctactg tttcatgcta taatctacat taaatgcact taattatatt
     7681 tcaaaggagt gttatagtat gtctttagaa aaccaactag ccgaacttaa atatgattat
     7741 gttcgtcttc aaggtgactt agaaaaacgg gaatctttga atttagatac ttccgcactt
     7801 gttcgtcaac ttaaagatat tgaaaatgaa attagaaacg ttcgcgctca aatgcaagat
     7861 taataatcta tcattcaagc aataaatgct tttcgttaca taaatttgac tagcattgct
     7921 ctgaatacgt tatattgatg aattgcttta tttttcgctc aattacatct agaatcacaa
     7981 gatgttgtcg tgttatgatt tagtgtttca ttaacaacat acacgcatat ctatcccaac
     8041 actgctattt atgttttcta cgctgctgta ctacatgaac cctttgaaac ggagaggaag
     8101 ttatcatatg caattttatc tgattttact agcaatactt tatctaattg ttagttttat
     8161 tagtattttt aaaatggaag ttgtatttac tcgcattttg agaattatta tgggtgtgtt
     8221 gttattattc gtcttagcat taacgacgat gagttttcca aaagagaatt ggtgggtatt
     8281 tatcgtctta ttactcttag tcggtaatgt cgaagtgaca ggatttaaaa tgcttaaaaa
     8341 agatctaaaa ggtgtaaaca tcttgaattt aatgtcatta tttatctttg tcatatattt
     8401 catcttaacc atcgtattat tctaagtaat aaacaaaaca tgcaataaca taattgcaat
     8461 tttattttat tatcgtacat gttgaatgcc agcattgttt tattaacaca tcgattaaat
     8521 cgcacgtgaa ataagatcta aaatttcaaa ttttaaaaag ccataaaagt agtcaatatc
     8581 atgaccgctt ttatggcttt tatattatct atcgtttagt gaatcgtttt ttcaaagtaa
     8641 agagaatttc atatattact ggtacaacta caagtgttaa taaagttgat gaaattaaac
     8701 caccaataac tgtagcggct aatcctttcg aaataagaat cgagctatct tgaccaaata
     8761 acaaaggaac taatgcaccg attgttgcaa ttgccgtcat taatatcggt ctaattctag
     8821 taccgcctgc ttcgattaat gcttctttca tctccatgcc ctgctgctca ttattaatga
     8881 cacggtctat taacacaatg gcatttgtta ctacgattcc aattaacatt agcataccaa
     8941 ttaaacttgg tactgatatt gtttctcctg tgattaatag tgcaataatt acaccgataa
     9001 ctgtaaatgg taaagagaat agaattgtaa atggtgctaa gccaccttta aatgtaataa
     9061 ctaggattaa atatacgata atgatcgcag ctaacattgc aaaggctaat tgtgtcattg
     9121 cattgttaat atcatctgat gcaccaccga tattaacctt tacattattc ggtttgtcca
     9181 aattatttat tttagacatc acttgtcgtg ttgtgccacc cacatcttta tttgttactt
     9241 tagcagatac cgtcgttgca taatctcctt gttcttgcgt caatttactt ggtgtcgttg
     9301 ttttaactaa cgtagcgata tttcccaatt taatcgtacc accagtcggc tttttcaaag
     9361 ttatattatt caacttatct tctgaccagt ctgtttgctt attttgtttg actttaacat
     9421 caacagtttt accattttct ttaacagtcg taactgtttt ttctggtaag ttttcattca
     9481 agtgcattgc aagttgactt gcagaaatac cattttctgc cgctttattt tgatcaactt
     9541 taatttcata ctggtcatat gtttgcgata aatcagattt gacattggct agtcctttaa
     9601 cctgtttcat tttctgttca atatctttta cagttgattt tatggcatcc attgatggac
     9661 cttttacagt aacctctaca gatttattac ctgcacctgt tcctaaatct tgatttttcc
     9721 actctccagg atgtttaaag ccatctgcat gtttaataac cttatccgct tctacatcaa
     9781 aattaggcgt gtcattatca tattcaacca tgattgccat actatttgta ctacccgttg
     9841 gatctactgg actactacca ccaactgagt attgaattgt ttttacatgc tttttctgtt
     9901 ttaaatattt ttcaacatct ttcgcatgat tcaacactgc ttgctccgtt tcaccaggct
     9961 tcggtgtata agtaatagct aaaaatttat cgtcacctgc tgaaataaag ctagtgccta
    10021 gtctcggtcc tccaaataca atagttacaa ccaaaattaa agtacttaat ataattacaa
    10081 tccacttatg atttagtgac caatgcaata cttttttata agttgtacta acaacaccta
    10141 atccttcttg atgttgttta ttacgacgtt taacgccttt tttaaatagt gttgctgcca
    10201 aagctggaac gagtgtaatt gacactaata acgatgctaa taaactaaat gcaatagcca
    10261 atgcaaaagg tctaaacatt tcgcctactg aacctgatac aaacacaagt ggtaagaaga
    10321 cgataatagt aactagtgtc gatgacatta ttggtttaaa tacttcagtt gtcgcactga
    10381 taattaaatt ttcacctttt aattgttctt ctgaatctgt taagcgtcga taaatatttt
    10441 caacaactac aatcgaatcg tctatcacac gtccaatcgc tactgttaat gcacctaacg
    10501 ttagtatatt caatgaaaca tcactcaatt tcagagcaat aagcgccata agaagtgata
    10561 acggaatgga tataatagaa attgccgtcg tacgaatgtt tcttaaaaac agcaaaataa
    10621 ctataattgc cacgattgta cctaatgatg ctttttcaac catcgtataa agtgatttct
    10681 caacaggctt tgcagtatcc attgtttttg tgacattaaa atctttattt tcatcaacga
    10741 atgtatcaat tttacgttgt acatctttgg ctacttgaac tgtattggca tcttgagctt
    10801 tagttatttg tagattaacc gcatcctttc cattcgtttt agaaatagaa gtacgcacat
    10861 caccaactgt aatatcagct aaatctccta gtttcgctgt cggcatacca cttatattat
    10921 ttggtgctga cgcttttgaa ttttgctgtg gtgatgcctg attaacgtct gacatggctg
    10981 aattttgttt attgtcactt tgggattgag attgcccttg tcctcctgca aacgttaatg
    11041 gaatatttat gtttttaaaa gcatcaacag attgatattg accatcaaca acaattgatt
    11101 tatctttatc accaaattgg aacaatccaa gtggtgttgt tcttgttgcc gtttttagat
    11161 agttttctac atcatcagca gtcaacccat atttttcaag ttcattttgc ttaaatttaa
    11221 gggtgatttc acggttcgtc tgcccattta attgcgcatt ttgcacacca tctaccgttt
    11281 gtaattttgg tattaattgt tcattcagta ctttcgttac ttttttcaag tcattctctt
    11341 tatttgaaaa tgaatatgct aaaaccggaa aagcgtccat cgaattacgt cttaattctg
    11401 gttgaccaac ttcatcttta aatttaattt tatcgatttc ttttttaagc tgttcttctg
    11461 ctttatccat atctgtatta ttttcatatt caactgttac aattgaagca ttctgtatgg
    11521 attgcgtttt aacatttttc acatatgcca atgatcttac ttgattgtca attttactac
    11581 ttatttcatc ttgggtactt tgtggcgttg cacccggcat tgttgttgta actgaaataa
    11641 ctggattttg tacatttggt agtaattcta atttcaattt agcactcgca tatacaccgc
    11701 ccaagacaac taaaacaacc attaaaaaga tagcaaactt attccctaaa gaaaattgta
    11761 atagcttttt tatcacagta cttccccctc tttcactata ttcaaaaata ttttactcaa
    11821 ccattctata ttgtgtaaaa aaatctgaac gcaaatgaca gtcttatgag cgttcagatt
    11881 tcagtcgtta atctattttc gttttaattt acgagatatt ttaattttag cttttgttaa
    11941 acgcggttta acttgctcaa ttaattggta caatggctga ttcaatacat aatcaaattc
    12001 accaatcttt tcacttaagt atgttcccca cactttttta aatgcccata atccataatg
    12061 ttctgagtct ttatctggat cattatctgt accaccgaaa tcgtaagttg ttgcaccatg
    12121 ttcacgtgca tacttcatca tcgtatactg catatgatga tttggtaaaa aatctctaaa
    12181 ttcattagaa gacgcaccat ataagtaata tgattttgag ccagcaaaca ttaatagtgc
    12241 accagaaaga taaatacctt caggatgttc cttttctaaa gcttctaggt ctcgttttaa
    12301 atcttcattt ttagcaattt tattttgcgc atcattaatc atattttgcg cttttttagc
    12361 ttgcttttca gatgtttcca tcttctgctg ccatttagca atttcggcat gaagttcatt
    12421 caattcttga tttactttcg ctatattttc ttttggatcc aactttacta aaaatagttc
    12481 agcatctcca tcttcatgca acgcatcata aatattttca aagtaactaa tatcacgcgt
    12541 taagaagcca tcgcgttccc cagtgatttt cattaactca gcaaatgttt ttaaaccttc
    12601 tctatcagat cgttccactg tcgtacctcg ctttaaagcc aagcgcactt ttgaacgatt
    12661 tcggcgttca aaactattta ataactcatc atcattttta tcaattggtg taatcatagt
    12721 catacgtggt tggatgtagt cttttgataa accttcttta aatcctttat gtttaaaacc
    12781 aagcgctttc aaattttgca aagcatctgt acctttatca acttcaacat caggatcgat
    12841 tttaattgca tacgctttct cagctttagc aatttctttt gcactgtcta acaatgcatt
    12901 taacgcttct ttattactat aatcaacaac aaaaccacgt gaaatataac atagcgtata
    12961 cggtaattta ggtacttttt taaaaagtaa ctgcgcaaca ccctgaactt caccgtcacg
    13021 acctacagcg attcttcgcg cgtaccatcc agttaatttc tttgtttctg cccatttcgt
    13081 taattgtaat aaatctccat tggggtggga tttaacaaat gcgtcatgtt cctgattagt
    13141 gatatgcatc ttttccatga tttatgatat ctccttctat ttaacaatac ctttaattat
    13201 acagtttgta tcttatagtg tcgattcaga gcttgtgtaa gatttgaact cttatttttg
    13261 aaatgtccat gctccaatta atagtttagc aagttcaaat ttacccattt taattgtgaa
    13321 tcattttata tctatgtttc gtgttaaatt taatgttatc gtacaattaa tacttttcaa
    13381 ctagttacct atacttcaat atactttcat catctaacac gatattcatt tctaaaatga
    13441 accaacttga cttcaatgaa taaatttttc ctcaagcaac cacattaatg ttcatataca
    13501 attacccctg ttataatgtc aataatctaa caatgaggtg tttgatatga gaacaattat
    13561 tttaagtcta tttataatta tgaacatcgt tgcaatcatt atgacattga gtcaacctct
    13621 caccgtgaat tactttagtt tacgggttat acttatcttt ttcacattta tattatcaat
    13681 ctttttcatt ttaattaagt catcacgatt aaataatata ttaacgattc tttccattgt
    13741 gcttgccatt attcatatgg gcattctcgc tcatagcact tacgtatatt tatactaatg
    13801 gttcaaagcg ataaatagca cctctgataa aaattgaata tggtgaagtt gcttgtgcgt
    13861 cttttatgat aaccgaatga tattttgaaa ctttaccatc ttcaattcta aaataaatat
    13921 catcattttt taaaatcaaa tctgtgtaat ggtcattctc ttctacaatg tccatatcaa
    13981 accatttcaa ccaattcgat actgtttgtg atcggttttt acttttcaca ataacagttt
    14041 caattgaaaa ttgtttttga aaatattttt gcaatttttt agtacgcatg gaatcacttt
    14101 cttcccattg aataaaaaat ggtggcttaa tttcatcatc atcctgattc attatataaa
    14161 gcaattgcca ctttacctta ccatctttat gtgtatctct ttccatttga atcggaccta
    14221 ctacttcaac ccgctcactt tgtagtttat ttttaactgc ctcaatatta tttgtacgca
    14281 aacaaatatt tttaaagcct tgctcatact tctcttgaac aatttgagta gcaaaagcga
    14341 ctccaccttc tatcgttttt gccatctttt tcaacttttc attattttct acatccagta
    14401 gctcaatata attttcattg atataaccta atttattgaa tgttccatat ttatgatgat
    14461 acccacctga atgtaatttt ataacatctc ctggaaaact aaaccgatct aactgatcta
    14521 tataatgaat gatgtgatca aatttcaata tcattagtat ccccctattt acatgtaatt
    14581 acgcttattt taacaaagta taaatatttt tgctcttaat aattatatat tgatttctaa
    14641 ttgctcccgt tttataatta ctattgttgt aaaaaggtta gctaagctaa ctattttgtc
    14701 ttaggagatg tcactatgct atcacaagaa tttttcaata gttttataac aatatatcgc
    14761 ccctatttaa aattagccga gccgatttta gaaaaacaca atatatatta tggccaatgg
    14821 ttaatcttac gcgatatcgc taaacatcag cccactactc ttattgaaat ttcacataga
    14881 cgtgcaattg aaaagcctac tgcaagaaaa actttaaaag ctctaataga aaatgacctt
    14941 ataacagttg aaaacagttt agaggataaa cgacaaaagt ttttaacttt aacacctaaa
    15001 gggcatgaat tatatgagat tgtttgtctt gatgtacaaa agctccaaca agcagtagtt
    15061 gccaaaacaa acatttcgca agatcaaatg caagaaacca taaatgtgat gaatcaaatt
    15121 cataaaatat tattaaagga gacacacaat gactaatcaa ggctcattaa ataaatcacc
    15181 tattttcacc aaaagtttta caatcaactt ttttgtcaac tttatcgtat acctatgcat
    15241 gtatttgtta cttgtagtta ttgctggcta tagtaagcaa gctttcgacg cttccgattc
    15301 attagccgga ttagttgtag gactatttat tgtcgggtct ttaattggtc gatttgctac
    15361 aggtaaattt gtaaatcaaa tcggacctaa acgattatta tttattggtt taattgcttt
    15421 aattatcacg caattacttt attttattga tggatcttta gcttttttaa tctttgtacg
    15481 tctcataaac ggtatcgcaa ctgcagttgt gacaactgca acaggtacta ttgcggcata
    15541 cgttacccct gttaatagaa aaagtgaagg catcagttta ttttccttaa gtttagtatt
    15601 aggtacagcg attggcccat ttctaggcat gttacttatt acaaaatacc caattgattt
    15661 actgtttatt atttgtgtca tattaggcat actcggactc attatctcat tatttataaa
    15721 agttgatttc gaagtaacaa ataataaaac tgaaactaat gtcattgata aaccaagatt
    15781 tagtattcat caatttattg caaaagaggc cattcctgtt gctgtaatta tgcttttaat
    15841 tggcgttacc tatgcgtcca tcctaactta tttacaagca tttgctttag agcgacacct
    15901 agtaactgca gcaagctatt tctttatttg ctatgctata gcatcgttaa ttactagacc
    15961 tattgctggt agattgatgg atgataaaaa tgaaaatatt attgtctacc cagcatttat
    16021 catgcttttc ctttcatttg tatgcttaat ttcaagctat caaagctggt taatattact
    16081 tgctggtgct tgcctaggtt tagggtatgg aaatttatca tctgcaatgc aatctattgc
    16141 gataaaagtc tcacctccga ttaaatatgg tatcgcgaca tctacttttt acgttggtct
    16201 agatgcaggt gtcggcttcg gtccatcatt ccttgggtta tttactcata tgttttcata
    16261 tagtgaaatc ttcggcttta tggccgcttt agcaatcatt actatgcttg tttacttctt
    16321 aattcatgga cgtcatgtaa ctagaaatgc catgaactaa atatttatat tcaaaattaa
    16381 aaagcatgcc aatctctatt catcatttgt ctgtccccaa gacgcaatta tgatgtactg
    16441 attggcatgc tttttaagtt tttagtttgt aacgcttaac acttctattt ctttagtaat
    16501 tttatctaac aatttctttt gagaagtact atagaaatag aatacttgtc tttcatccgt
    16561 ttcagaacgc aatttagaaa taagctcttt tttatagatt ttacggataa tcacatcgat
    16621 tttacttgta tcccatagca tttcaaaatg taatgtatct ctcaattctt taccgctaat
    16681 cttttcagct tcatacactt taaaaagtac tacaaattct tcaattgaaa tcttatgtgt
    16741 tgtttttaac cagtgcttta actgacttaa ctccctttct gcttctataa aacgttgaac
    16801 tttattactc ataatatgat gcctcctatg tttcttctta gatgtttgta attcatatta
    16861 ttatacaatt acttaaattt attatatatc aaacttagca ttttatctat tacaaatatt
    16921 aaattgctgg ctatatcttg tttagattta tatcttttgg acaacttgtt gtttattcga
    16981 caacatttat acctaaaaaa gatatattac aactcaatgg catggagtgt gacatcaatg
    17041 ttaaagaacc actaaagatt tattatgtag tgattcttaa gagtgggata gaaatgatat
    17101 tctcataaaa tttatttcgt tgttccccaa cttgcacatt attgtaagct gacttttcgt
    17161 caccttctat gttggggccc cgccaacttg cacattattg taagctgact ttctgtcagc
    17221 ttctgtgttg gggccccgcc aacttgtaaa aactttaaaa acgatcattt ctattaaatc
    17281 tggcatagat atgaccgttt ttactttaat aaataatatt gtattttagg gagtaagaca
    17341 gaaatattaa aacatctcta atgatttaat atgtagtggt ccctacacat taaccaccaa
    17401 tataattcat gtttatcttt ttacgttgac gattggcaac tgtttgagca gttctctcat
    17461 ctgaatatcg atcatctctt atttgccata aagctttaaa ttgttctttt aattcttcgt
    17521 cggtcacgcc agaacgaata aacgctttaa cgttaaatcc atcgacagtt gcaaataaac
    17581 atccgtaaaa cttcccatct gatgacagcc ttgcgcgtgt acatgtagaa caaaatgatt
    17641 gtgaaacact tgtaatcaaa ccaaattgaa caccattatc cttatggcga taatattttg
    17701 ctacttcccc aaaatatttt ggttctacag gatcgatttc aaagtgctgc tctatcattg
    17761 taagcatttc atctttagtt acaactttac tgaaatccca tccattatca ttaccaacat
    17821 ccataaattc tataaatcga atctctatat gtttatcttt aaaatattca agcattggta
    17881 tgatttgatc atcgttaata cctttttgta taacaacatt tacttttaca ttcaaaccaa
    17941 tagacgtcgc gtaatcaatt tgttctaaaa tcgtagtcgc tttaatatta cgattattga
    18001 ttgattgaaa tagcgtatca tcaatagcat ccaaactgac attaattctg cgcagcccag
    18061 catcatataa cttttgtcca tgctttttta ataacaaacc atttgtagtc aaaccaatat
    18121 cttcaatacc atcgatttga tttaatttag ctataagtac atctaaatcc cgtcgcatca
    18181 atggttctcc acctgtaatg cgtatttttt ttacacctaa ttctgcatat accttagcga
    18241 ttctagccat ttcatcaaac gttaaaagtt catttttagg taaaaatacg aaatcatctc
    18301 caaatacctc tttaggcatg caataatcac acctaaagtt acaccgatct gtcacagata
    18361 accgtaagtc acggatggga cgtcctagtt tatcttttat ttgttctacc atttgtggac
    18421 ctcctaacag cttacaattt ttgaattaaa gcgtccaaat catgctgata atttatattt
    18481 ttgtaccaat atgagggcgc atctacatcc cttacatcca aataatccgt tgataattca
    18541 tgatatacat ttttaaaact gtaattatca gaatgtagtg ctttagttat agcgcctaat
    18601 gcattcggac tataaaatgc aattgttgga ataaaacgtc catcttcttt aaaagctgcg
    18661 acatctaaat gattttcaat aagatgagaa actaaaaact gatacaacgt gcttacagct
    18721 ttaccagtaa tcattggtgt atcaacagaa acgacaaaaa acaattcttc ttcaggatgt
    18781 tgcttcatga ttgtataaat tcctgctaat ggacctttat cattatgatt ctcatcatct
    18841 ataacaacat ttggatattt aaattgcgtt gccaattgcg catttgtact aataataatt
    18901 tcattgaaca tatttgttga ttctaatgtc ttaattactc tactataaaa ggtctcaccg
    18961 ttcacttccg caaaagcttt gggcttacca aatcgcactg aatgaccacc tgcaagaatt
    19021 attgctttca tgctccctta acctccacta accggtggaa ttaatgcaac agtatcatta
    19081 ggttgaatga aatccgattt ttgtacaaat tcctcattta cagcaacttg aaacttttta
    19141 ttattgattt gcggataacg ttcaaacaat aaatcttcaa attgttgtac agtcaatgct
    19201 tgttcaagca caatatcttc ctgtgctttt tgtaatatat ctttaatttc tgcgaagtaa
    19261 agtaccttca tctctcttat tcctccctct tcgcttcttc ataattccct ttttgatgcc
    19321 cttgccattt tgaaccatct tcccaaattt cttttttcca aatcggaaca atttctttta
    19381 tacgctcaat tgcatattca tttgctcgat aggcatcttt acgatgcggt gaagaaaccg
    19441 caattaatac agcgatatct gaaatttgta atggccctat tctatgaaca atactcgtta
    19501 tcgttccagg ccatttttca tttatttcat ctccaatttg tgccaatttc ttttcagcca
    19561 ttggaatata cgcttcatat tctaaatatt ccgttttgac gcctttagtc cattcgcgaa
    19621 catgaccggt aaaaacaact actgcacctt gatattcatt tatagtgaat tcacgatatt
    19681 gttctgtttg tatcggttct gtcacgattt caaattgttt cattcatttc aatcctctat
    19741 gttaattgtg tatcacaatc atttttaatt ttatttaata accattgctc aaatgctgta
    19801 aaatcttcat gctcccttac attaatgcta taacaaacat tcgacaattg ttgtaatact
    19861 tgcaactctt cttcatttcg atagactacg actttttcaa aatcagcatt tttaaagcct
    19921 tcaactaata cgatattggt gtcaattgta acagattttt caataatttg agtaagattt
    19981 tgattatcta cacgtgttac agtttgctga tattgaaaac cttgtacaat actttgatct
    20041 gcccccgctt caaaatgctt catgtgatcg acgtctgaat cctgtaattg aatatcttcc
    20101 ttaccatgcc catgatgttt aatagtagca actgtataac catgtgactt taagaaagag
    20161 acaatatgcc tcatcaatgt tgtcttacca gactttttgt aacctacaat ttgtaaaatc
    20221 ataaaagaag ttcctcttca gcagcgtcag attcagtcaa tataatatct actgtatgcc
    20281 ccgctttaaa accacgtgac cctcctggta acatgaccat acagttagca tgtgcaatcg
    20341 ctacaaccgc acctgattta ttgaatcctg aaggtactac agtagctcca gcacttgtta
    20401 acgttgcttt agcacgtata aatcgtgtga atgggtttgc cttggtaaaa tcttccatta
    20461 atgttgcttt aattatttgc gggaagactt ctagtgcgcc acacatatgt ttaacagctg
    20521 gtttcacaaa tagttcaaat cctgtaaaac aagctgatgg atttccagat aatccaaaca
    20581 aatactttcc atctacaaat gcaaccgttg ttacgctacc aggacgcatt gctactttat
    20641 taaataacac ttccgccttt acagccttat aaatctcagg taaatagtca aaatctccaa
    20701 cagaaactcc gcccgttgta ataacgatat catgtttttc catagcttct ttaacgactt
    20761 ggatgccact atctaaatca tctttttgtg ttttgtaaat accaacttca agacctaatt
    20821 tttctgctaa ggcacgaatc attgggccat tagagttacg aatttttcca tcttctaata
    20881 catcattaac atctaataat tcgcttcctg ttgcaataac agcgacactc ggttgcttaa
    20941 taactttaac ctctgcatag ccatatgttg caaggaccgc gatagcccct ggattaatta
    21001 cttgtccttt ttttagaaca acatcgcctg tcgttgtttc ttcacctttt aaagatatat
    21061 tttcattttt tgaaaatggt ttacgaattg taaatgtatc ttctagttca atcgtttgtt
    21121 caaacataac aacagcatct gcgccattag gtatttgtgc tccagtcata atacgcaccg
    21181 cttcgtgatc cccaactaat ttatcagaaa ctgaacctgc accaatatga tcaatcactt
    21241 taaactcaat gcgattctga ccacttgccc cttgtgaatc aacactgcga attgcaaaac
    21301 catcataagg tgatttatca aaccttggta tatcataagt agctacaata tcttctgcta
    21361 agatatgatt tagacttttt tcaagtgcta ccgtaattgc cggcattgaa ctctgctgat
    21421 taacgatacg ttgaattgct tctttaactg ggattgggtt tcttttttct actaccatta
    21481 aaaatcatct ccaaaacttt tattttttca tgatatacta gatgcactag gaggggtaaa
    21541 atgactgagt ttactcatat taatcaacaa ggccacgcca aaatggtcga tgtatcagac
    21601 aaacaaatta caaaaagaac tgctgtcgct cattcaagca ttactgtaaa taaaactatt
    21661 tttaagcaga tttctaataa caccaatacc aaaggcaatg tattgaacac tgctcaaatc
    21721 gctggtatta tggctgccaa aaatacatca acaattattc cgatgtgcca tccattacca
    21781 ttgactggca tagatgttca cttcagctgg gatgaaacaa atgcacccct ttatacttta
    21841 aacattcaaa caactgtctc tacaactggc aaaaccggtg ttgaaatgga agctttaacc
    21901 gctgcatcag caacggcttt aaccatttac gacatgacga aagcagttga taaaggtatg
    21961 attattggcg aaacctacct tgaaagcaag tctggcggta aatctggaga ttttcaacgc
    22021 caatcaaatc aataaattat tttgtaagct catgaatcag atgatttaat tctggtttaa
    22081 tgagcttttc taatgctaat ttaactgcgc ctgttgatcc tggaatcgaa aatatcaatt
    22141 tattattaac tgtacctgct acagcacgag acaataatgc acgcgtgcca acatcttcaa
    22201 cataactcaa atatctaaac aattccccaa agccttctat ctctttagtt aaaagtggtt
    22261 ttactgcttc aatcgtcaca tcacgttgtg caatacctgt tccaccagtc gtaatgatga
    22321 catcaatatc ttcttctaac cacttcttca cctgcgtcgt gatggctact ttttcatctt
    22381 tcacaattgt ataatgtgcg tcactcactt caacgtcatc tgcttgtaat agttggcgca
    22441 cgcattgacc acctttatct gtatcaaagt ctctagtatc tgataccgtt agtacggctg
    22501 ctttaactgt acgattcaat ttaacgtttt gatgttcgcc cataatatta cactcctttt
    22561 gtcttatctt tttttatcca aacaataaat tcattagatg tgtggcatct gatgtgcgtg
    22621 tcatgccatg tattaaaaac cttccacctt taaaagcaac aatgcggtgt cctttaaatt
    22681 caaacataac catatacgaa ttactgcgat aatttagttg atgttgtttt aaaaattgaa
    22741 caagaatgtc gtgtgtaatt gatgcatttt catactgtac agtgtctcta ccacacaatg
    22801 ttgcataacg ttgttcattc ttgtttaaat acggataact tggtacatct ccacaagttg
    22861 tacagtctga acgttgcatt ttactgaaac caaatgaata atgactacca tcccaaatat
    22921 cgccataagt tatttttgtg tcaattggtt gttccgttaa tactttcatc gcatctctta
    22981 attgtaaact tgttgccatc gtcacggcag gttgaatgac ccctaccgta tcacatgtta
    23041 aatttaatgc tggcaattgt ggtaccaaac agttaaagca aggtgtttta ccaggtataa
    23101 atgcagcttc tgtatatgta ctctgtacaa caccaccata aatccaaggt atacgatgtt
    23161 tatatgcaaa atcattaatc agttgtcgtg tttcaaagtt atcggttgca tcaataataa
    23221 cgtcaacgtc ctgtccatgt gtttccaaaa aataataatc cacatgggca atataatcat
    23281 caatatcaac atcactacgt aacgctagca aatgcttttt agctgcaacc accttaggca
    23341 tcattttcaa agcatcttct tcagtaaaca atgtttgtct ttgtaaatta ctaaattcaa
    23401 tataatctct atcaacaatg attagtttag caatgcctgc tctaacaagt ccttcagcca
    23461 catgtgtacc taatgcgccc ataccaataa tcaacgcaca tttttgattt attttgcttt
    23521 gacctatttc acctatttgt ttaaataaaa tttgccttga ataacgttct tgactcacat
    23581 taagttatcc tttcttttta tcattacttt cattatatga cgcgcgtcaa tgtctctcaa
    23641 aaaaactgcc cctaaaaaag agtttaaaac atcattaaaa tgctgataga agcattcact
    23701 tctacaattt taaatacgtt ctaaactctt taatggcaaa taaatgatta cccaattgta
    23761 ataatttcat gagccatttg ttctgcttca tagtttgaat gtgtcacaaa tatgattggt
    23821 atttgccatt cgttgaaaat acgtttaact aatgtaatac tctcatcttt tgtagtatca
    23881 tccaaactag aaaaaggttc atctaataaa attaaatctg gtttcgtgct aagtgcacgt
    23941 gcaagtgcta cacgttgtgc ctctccacct gacaatgtca taggatattg tttcatcaaa
    24001 tgatcaatgt ttaaagtttg aattaattga tcgatgtgtt cagatggttc agccataaaa
    24061 gtaatatttt tatagaccgt catattagga aacaattggt agtcttgaaa cagatatcca
    24121 atacgtcgtt gttgaatttt aacattcacg ttttttgccg tatcagtaag taattgccca
    24181 ttcacttcga taatagcttc atctgcttta cgtaatccgg caatcatatt taaaacagta
    24241 gttttaccaa tgccagatgg accacgaact gcataaattt ttggttcagt atcatctata
    24301 ttgatgcgaa ttaaagtgtt ctttaattga tatttcacat tgattttaag catctagtcg
    24361 acctccttat atttatcttt attcaataaa ttaattgtag atataaccac aatagagaat
    24421 gcgactagca ctaataccca taaccacgct tcattttctc taccttgttc cactaagaag
    24481 tatatttcta aaggtagtgt attcgtttta tttggaatat atcctgcaac cattaatgta
    24541 gcaccaaact cacctaatgc acgagcaaaa ctcatcatta tacctgctaa aatagagcgt
    24601 ttagctaatg gtaaaattaa tttgaggaaa attttcgttt cacttgctcc catcgttcta
    24661 gctgtattaa tcattttcgt gtctatacct ctgaagcctt gcacagtatg ttgatacatt
    24721 agtggaaaac taacaatgac agatgctatc acagcacctg tcaaagtgaa cactacaggt
    24781 aaatgtagta cattcgcaaa gaattgaccg attggtcctc ttggcgagaa gatgattaat
    24841 agaataaaac ctaataccgt tggcggcaaa acaataggta atatcaataa actttccaat
    24901 actttaaccc acgaaccctt acgatgatac aaccatttag atataaaaat acctaaaacc
    24961 gttacaataa tcgtactgat tacagcaact cgtattgata tccaaaaagg tgttaagtca
    25021 ggcatggatt acaactcctt atgctgcaaa gtggtattct tttaatattt ctttagcttt
    25081 atctgatttt aagaattcca tccactcttt tgctaattta ctatctgatg tagcaccagc
    25141 ttcgtatgtg attggtttct taagttctac ttctttaatt acttttacag tatcaatttt
    25201 tttgttttgt ttatataagt cagttttata cacaaaacct tgtttcgcat tacccttttc
    25261 aacataattt aatacttgtt ttacatcttt agcataaacg attttacttt cgacttcttt
    25321 aaataagtta ttgttatcta aatactgttt cgcatatttt cctgctggta cagttttcac
    25381 ttcacctaat gctaatttat cattgtcttt taagtctttt actaaagtgt aatttgaatc
    25441 tttatcacca attaatacta gactattttt cgcatattta tatgtatcat gcgctttatt
    25501 cttgtctttt aatgcatcta catctttagt atttgcagac ataaatacgt caacaggtgc
    25561 gcctgattca atttgttttc ttaatgcccc tgatccacca tagttaaatt taatatcagc
    25621 atttttatgc tcttttttaa attctgaagc taatttcttg gttacatctg ttaaacttgc
    25681 tgccgctgca acttgaatct cttgtttctt accattgtct gcgtcatctt ttttactttc
    25741 attattatcg ttagaattag agcaaccagc taatactaaa aataatgcca ttacaatagc
    25801 tataaaacgt ttcattttca ttgagtgaac cccctttttt aataatattt acattttcat
    25861 tatacactta ctattatatt tgaaaagaac cgttgcctgt ttatatattg attcaaacaa
    25921 ttgtcaaatt tgcataagat tgatatttat taattgatcc cctagtatgt atactctcga
    25981 attcaattta aatcaaacag attgctcgct attatttttt ataaatcaaa tttcaaactt
    26041 aagcaaatat gttaaaattt taagcaaatt gctttaaaat aaagtttaag ctaattaaag
    26101 gggttagata atgaacaaag atgtatcttt aggtcaacca attgttagat atgaagatgg
    26161 aaaattattc aatacaacag atcaatatgt gacagaattt ccacttacaa ttatggtgaa
    26221 tggtgaagaa tttgcgactg ttatatgcag tccaacaaac cttgaggagt tagtcattgg
    26281 atttctggct tcggaaggtg ccattttaaa gcgagatgaa ttaaaatctg tgctgattga
    26341 tgatagtaaa ggttttgccc atgttgaact taataaagat ttaggcgatc gctttcaata
    26401 ttccacaaaa cgaatgattg catcttgttg tggaaagagt cgtgagtttt attttcaaaa
    26461 tgatgctgct atcgctaaaa catctatgtc taaaatcact ttaactccaa tgcaaattat
    26521 taacatgatg actcgattac aaagtgcaag tcatatttac caagaaacag gtggcttaca
    26581 taatgctgct ataagcgatg gtttgacatt ttttgtacat cgacaagaca ttggtagaca
    26641 taacgctcta gataaattat atggattttg tattcagcga catattactg tccgagataa
    26701 agtattaatt tttagtggac gtatttcatc agaaatttta attaaagccg caaaaattgg
    26761 tgttggcgtc attttatcta aatcagctcc gacaacgctt gctgtaacct tagctaatga
    26821 cctaaatatt acggcagtag gatttattcg aaatggtggc tttaatatat atagtcatcc
    26881 agaacgtatc atcgactctg aacaataaca tattcattat agtgtgtcat aatgaacgat
    26941 attctacatg aatcaacaat ataaaagcac tatttttata agtataagta gctcaaaaca
    27001 atttaaaaag cagtaagttg atttctactc gaaaccagct tactgctttt ttattctaat
    27061 acaaacctat catcaatcgt taatttccaa tatcttcttt taatatttga taacaaaatg
    27121 attggtaaac ataaccttgc tttgcataca tatcttttgc tgtatcttca ccatctgcaa
    27181 ctaatatgat tggtttgtga ttttttgata tggcgtattc agctatcaac gattgaattg
    27241 tagatccaat tccttggtgc cgaaattgtt ctaatacacc aaatccatct aattcaatgt
    27301 aattttcact ttcaatgaca tctacaacgc caataggttc attatttaaa taagctacta
    27361 agcgtttaat cacatctttt tgataatgtt cccttaccat ttcttcatgt gcatctgcat
    27421 agtcttttcc aaatggcaaa ctaaactgat atgcaacttt taaataggca tctatatgcg
    27481 actcgtcaac gatggcaatt tcaacgtcag agttacgcgg caaattggca agcgcatcac
    27541 tttctatggc atacaattcc ataataccta gttcaaaatt catatcttta aacatagcca
    27601 tccaatgatt atcaagttgt tcattttccg ggaaactaaa cgcataatga tttgaacctt
    27661 gatttgtatg taattgatgt tgacgttcta agtctttcag ccatcgctct tgggtaggcg
    27721 tctttttata tatccatgtg ttgcttgtgt atcgttgtgg aaaagaaggc gttaaataaa
    27781 ttaactgctc gtcgtcattt tcaaatatag tgccgttaca atatatatct gacatactta
    27841 tactagccat tgacacctac tcctttcact tttataaatt tcaaagcaac ttattcaatt
    27901 actttattat cttaacttaa atatgattat tttaatccca taatttgacg aaactgcgag
    27961 tgattaagta aagctgtacc aatcaaactt gctacaattg cttttaatat atcaccaggc
    28021 aaataagcta atgaaattga aatagctttc gtaaatggta tgttaataat catgcccata
    28081 atcaatgtac caataacatc taatgctata acaccaaaaa ctaaaatacc aacgaataaa
    28141 atccagaaat taatttcatt gatgaatcta tcacgaatcg caccaatcat gaatgctaca
    28201 actggatata atagtaaaaa ccctgctgaa ggacctgcga atacaccaat gccaccgcga
    28261 ccgcctgata acagtggcaa gccagcaact actaataata aaaagacgat aacacttaat
    28321 gtgccatatt tacgtcctaa aatcacacct gctaagaaaa taccaatgtt ttgaagtaca
    28381 attggtactg aagaaaatgg tagtggaatt accggtacca atcctaaaat agcgataatc
    28441 gctgtcatta aagctgtata tactaactgt tttgttgtca ttttttactt cctttcgtat
    28501 ttcatgacca ttattatagc accattgtaa actttaagaa acgaaaagtt tacaataagt
    28561 tgttctatat aaattaaaac cttaattatt ctacacagct gttaatttac ttttttagca
    28621 agtgcagtta tataatcaaa aaatgcgttg ttatctacat gatttatgac attaattttg
    28681 cgcccatctt tacactcaaa tgtcttacct tgacttggtc cataacttat tacatcgact
    28741 tttttctcaa ttgaatgaac caagtccttg ttaccaatat aagcagtcgt taaaacatcc
    28801 cataaaaagt aagtagaatt tgttataaag tgtgttaatg gtggtaccgc tgcataactt
    28861 actcctaaaa aatcaatgcc cgtatattgg cgttcattcg cccatctttg tctaacatct
    28921 aacgttagcg gtacttgatt cgtactttct aaagcaacca tatcaatctc tatatcgcta
    28981 tcaaaaacaa ttttaaccgc ttctggatcc caatatgcat tccattctgc agaaccatca
    29041 tgctcaggtt cttcaacatt tccttttggt aaaaaggtac cacccatcca aactaatttt
    29101 tctatatact gaacgataga tgaatctttt tgtagtgctt ttgctaaatc tgtaagcggg
    29161 cctgtaaata ataatgttac tttttctgat tgtctcttta aagtttgaat aatgtcttca
    29221 aaggcttctt tgtcgctcac atttgaagca acatgtttga ctggctcatt taaaattggc
    29281 aatgcgtcca taaaaaaggc atgcatacgc cattctttag gaaatggatt ttttcctcgt
    29341 tcatatgatg gcgcaacttg aatatcttca tttgaaaaac gattaataat ttttactgat
    29401 gcgctcaaag atggctctaa ataacaatca gcaccaattg tactgacccc tatcaattga
    29461 acgttttcca tttgtaataa taaaaataga gacactaaat catctacacc accatcatgg
    29521 ttaaaataaa ctttcttagt cactcgaaat cccccttaca tttcattttc cattaacaat
    29581 attatgtttt ataaaacgtt gttatatatc tttctacaaa atcataacat tctgttgtaa
    29641 tccttttaaa cctcttcttt tattgatagc cattccctga tatcccttcc ataaagtatg
    29701 aactaatata attatgatat aacttattaa taaagagata cattgagcta ttttctcagg
    29761 tatactactt tagctataat aatactaatc tatcattaat taaattgaac gataacaacg
    29821 gcacttatta ttaaaaaaaa tacaaatatc taactttcta gctataaaaa taagagccta
    29881 atacaatatc aaaagattga tatgtttcag actctcattt cactaactta tatagctgaa
    29941 ttattttgca gctttaatta atttttcttt taaatcttta cgcatgaaat ctaatgtata
    30001 aggatcgttg taccagtatg taccagcatc aactttaaca atatgtcctt ctttagtagc
    30061 tttcaaattc ttccacatgt ttgttgattc atatcctggt gtaggtttac cttcacttgt
    30121 actcacaatg taatcaccag catatttttc aatttcttct tgtttcactt cagcccaacc
    30181 tgcttttgca gttaactttt gttgttctgg ttgcattttt aaaccaaatg cttgatataa
    30241 tacttctcca ccacgacccc agttatcgcc gtaagtgtat aattttttat caaattcatc
    30301 aaacaatgac actgttgcat cttgtccaat tgctttttta atttctttac cgtctttagc
    30361 agttgtttct tcccaatctt tcttccaagc ttttacttta tcttctttac caacaatttt
    30421 ccctaacatt tcttgttgtt ctaaatattt atgcttatta tagtcaacaa ctactgttgg
    30481 tgctactttt tgatattttt taatatcttt gtcagtagag tatacaataa ttaaatctgg
    30541 cttttcttta gcaacttttt ctacatcgcc atcaccaatt tttgtaacac ctttaaattt
    30601 atcttttaat actttgcttt gatcgacttg ttgatttaca gctacaatgt ttgcacctaa
    30661 ttttttaagt ccaccagcat atgttggcgc aactactgca atgcgtttag ggtctttcgg
    30721 aatatctacc gttttgccat cgtccatttt ataagattta gtttcagctt tgttattttt
    30781 ttcaccttgg ttcccacacg cagctaacac taataacata attattaatg gtaataatag
    30841 ttttttcata atttcctcct attgaaaatg attatcaatt attccaaaat tatacaagat
    30901 tgcatataca tttacaatag ctagtgctat ttattttttt attttcgcaa aaaatttaac
    30961 aaaatatctt aacatcccta taaatcccca caaaaaaggg taaatccgaa cgtcaaattt
    31021 catctcttta tgccaatcaa cgatcgattt acctttttaa atttaaaatg tatctgtaat
    31081 actcttagca atatttgtat atgctgcatc ttccataggt ggattgtgta atcctgcacg
    31141 catgtcacga tagtaacgtt gtaatggtcg attcatttct aagcttttag caccaactat
    31201 tctcatcgct aaatcaatca cttcaagccc ttgattcatt accatcactt tactcgcact
    31261 tgttgaattt cttatctgac tgtcatcttt atattgttga tagccttttg ctgtactcca
    31321 taaaaattgt ctcgcacata acaatagcgt ttccattttc cctaaatttt gttgcacagt
    31381 tggcaatgtt gcaatcgttc cttcaatact attagggcta tgttggattg caaagtctac
    31441 agcataattt ctagcagctt gtgctatacc taaataacag cttggtatat gcaatatcca
    31501 accatttggc gctttacttt tctctgtttc cactaaatgt tttaatggta cttttacatc
    31561 atttaatatt aaatcatgac tttctgtcgc tcgcatacct aatacgtccc aattatcggc
    31621 aatttcaaca ccaggcaaat tcctgtccac taagaaaaaa ccaacacctt ctaattcttc
    31681 tatataagca gcaacaataa tatgtgttag tgctttactc attgatgtat atgtcttcac
    31741 accatttaaa atatacccat catcagcttt aacagcatgt gtacttggtc ttccccctct
    31801 tgttggacta cccatttcag cttcactaac tgctctatta actaatgcac cattattaat
    31861 ttcaacagca aattgctcca acatatcttg agaccataat ttctgttcat aaatttgtcc
    31921 tacgacactc acatgccaac caatagataa tgctgtggca ccatcaagtt cgcctaaaaa
    31981 tgattgcaaa ataaccatgt cttctatggt cgcaccttca ccaccatatg cttttggtaa
    32041 cgttaatttt ccataacctt ctttaattaa ccattcaata tttttatatg gaaattggct
    32101 atgttgatca ttatattcag ctttagcctt aaatgtatct ctaattactt tgaatttttc
    32161 aatccatttt ctttgaattt ccgttgttat taaaaaggaa ttatccatat cattcatatt
    32221 agtcacccca cacaacacac aatataaatt atttattcct agtttaacac tcgactttaa
    32281 aaataaaaag gaacgtgctg tgaacaatta aaagaaaact gataattcga ttaagtatgc
    32341 acttaatttt atccataaaa atacacctta aactatacaa ttcgctgtat agtttaaggt
    32401 gatattcaaa caatattagg tttgatttaa ctatcaagca tctactttgt catgtttaat
    32461 accagcaaat aataaaatcc atgtcacaat aataaatggc aatgttaatg caggtaagcc
    32521 aaacggttca agcaatgttg ttgtacctag ttgcaccact actgttaata acacacctaa
    32581 aaaagtcgaa atataagggt taatcgctgt tttaaatgta tatcctagtg cgattgccat
    32641 taatacaaag ttataaccga ataatccctg attaatatca tcataattac cacctaatag
    32701 agctacaatg ataaagctta acaaactagc tataacagct aagagtgctg cttttcttga
    32761 tgctatcaat atgccgatta aaatacatac gccaccaatt acactcgctt cgataaatac
    32821 ttgactgaat ccttcaaata atgactgaat gaaatgtatt ctatcattgt tgctaaaatt
    32881 aaccgtttca atgttttgag gcattaactt taacgatgta tctacaaatt taacctgtcc
    32941 tgaaagtaaa attgtaaacc aagtcacaat gacaaaaggc atcgtcagca tcggaacttt
    33001 atatggtctt aaaacttctc tcactgcagc agcaactggt aacgttaata aagttgctat
    33061 cattgttata acaatatcta atcctgactt atctaaaaag attgttaacg caatggcagt
    33121 taacactgga ttaaatccgg ctaacccatc attaatctct gcttcactat aattaatgta
    33181 acgtgcaaat gcataagcta tgatactacc tacaatagcc gctaatccaa ttgtccaatc
    33241 ggctacaaat aatcctatta agataaataa tcctgtccat ttattactaa ttaacacaac
    33301 ctgagaaata tttttcaaaa gtacatctat tatcttcaaa tctttcacct ttcctttgaa
    33361 tcattactca ttaatatact actattctat gaaaagttat attaaaaact attttggttt
    33421 ttacataaat atattccact atttgttttc gattagattg tttgcttcaa tttcggatat
    33481 gctaaaaata taattttcta attacacatt gaactttgta tattacttgt ttatcacaac
    33541 aattaggatt aatataaatt ttgtaaattg tatctaatat gaaattgggg gttttaattt
    33601 gcattttaca caacgagagc aagacaaatt aatgattgta gtggcggcgg aagttgcacg
    33661 tcgtcgtaaa gcacgtggtt tgaaactaaa tcatcctgag gcattagctt taatcagcga
    33721 tgaattatta gaaggtgcac gcgatggtaa gaccgttgca gagttaatga gttatggtag
    33781 acaaattcta aacaaagaag atgtcatgga tggtgtcgaa cacatgatta cagatatcga
    33841 aatcgaggct acgttccccg atggtactaa gttaatcaca gtacatcacc ctattgttta
    33901 aaggaggcag tcaaatgata cctggagaaa ttattacaaa aagtacagag gttgaaatta
    33961 ataaccatca tcctgaaaca gttatcgaag ttgaaaatac aggagatcga ccaattcaag
    34021 tgggctcaca ttttcatttt tatgaagcaa atgcagcatt agatttcgaa cgtgaaatgg
    34081 catatggaaa acatttagat atcccagctg gagcagctgt ccgatttgaa cctggggata
    34141 aaaaagaagt tcaattagtt gaatatgctg gcaaacgtaa aatttttggt tttcgtggta
    34201 tggtcaatgg tcctatcgat gagtcacgtg tctatcgccc aactgatgaa aatgatgaat
    34261 atgcaggtgt attcggagat aacggtgctg aaaacgtgaa taaaaaagga ggaaaaagat
    34321 catgagcttt aaaatgacgc aaaatcaata tacgagctta tacggtccaa ctgttggaga
    34381 ttccattcgt ttaggtgata cgaatctatt tgctcaaata gaaaaagact atgcggttta
    34441 tggtgaagaa gctacttttg gtggtggtaa atctattaga gacggtatgg cgcaaaatcc
    34501 tcgtgtaaca cgtgatgacg tgaacgttgc agaccttgtc atttctaatg ccgttattat
    34561 cgattacgat aaagtggtta aagctgatat aggcattaaa aatggttata ttttcgccat
    34621 aggtaatgcc ggcaacccag atataatgga taatgtcgac attattatag gttcaacaac
    34681 agatatcatt gccgctgaag gtaaaatcgt cactgctggt ggtattgata ctcatgttca
    34741 ttttattaat cctgaacaag cagaggtcgc attagaaagt ggcattacga ctcatattgg
    34801 tggtggtact ggtgcttcag aaggttctaa agcaacaact gtaactccag gtccatggca
    34861 tattcataga atgttagaag ctgccgaagg tttaccgatt aatgtcggtt ttacaggtaa
    34921 aggacaagca acaaatccaa ctgcactcat tgaacaaatc aatgccggag caattggatt
    34981 aaaagtacat gaagactggg gtgcaacacc atctgctttg agtcatgcat tagatgttgc
    35041 tgatgaattt gatgttcaaa ttgcattaca tgcagatact ttaaatgaag caggatttat
    35101 ggaagacaca atggctgctg ttaaagaccg tgtacttcat atgtaccata ctgaaggtgc
    35161 tggtggcggt catgcgcctg atttaattaa atcagctgca ttttcaaata ttttaccttc
    35221 atctacaaat ccaactttgc cttatacaca taatactgta gatgaacatt tagatatggt
    35281 aatgattact caccatttaa atgcggctat tcctgaagat atcgcattcg cagattcacg
    35341 tattcgtaaa gaaacgattg cagcagaaga tgttctgcaa gatatgggtg tattcagtat
    35401 gattagttcc gattcacaag caatgggccg tgtaggtgaa gtaattacac gaacatggca
    35461 agtagcacat cgcatgaaag aacaacgtgg tcctttagat ggtgattttg aacataatga
    35521 taataatcgc atcaaacgtt atatcgctaa atatacaatt aacccagcaa ttacacatgg
    35581 tatttctgaa tatgtaggat ctatcgagcc gggcaaacta gctgacattg tcttatggga
    35641 cccaattttc tttggggtta aacctgaatt agttgtaaag ggcggattaa ttaactctgc
    35701 cgtaaatggc gatgcaaatg gttctatacc tacatctgaa ccgatgaagt accgtaaaat
    35761 gtatggtcaa tacggcggaa accttacaag tacgtcaatg acattcgtgt ctaaaactgc
    35821 ttatgaaaat ggtatcaatc gtgcattaaa tttaaaacgc atggtgcgtc cagttaaaaa
    35881 tattagacaa ttatctaaag cagatatgaa aaataacagc gcaacaccta aattagacgt
    35941 tgatccacaa acatatgaag tatatgtaga tggagaaaaa attacaagta atgcagcaac
    36001 tgagttacca ttaactcaaa gatacttctt attctaggag gaacatagaa tgattgttga
    36061 agaaatccaa ggcaatattg ccaatttgtc aaattcagaa aaacaaaagc acgtcgaaaa
    36121 agtatacctt gaaaattcag atcttgttaa acgtattcaa agagtcgtta cagaccatgg
    36181 cactgaaata ggcattcgtt taaaacaacc tattgactta caatatggag atattttata
    36241 cgcagatgac cataatatga ttattgtgga tgttaattca gaagatcttt tagtgattca
    36301 accaagaaca ttgcaagaaa tgggagatat agcccatcaa ttaggaaatc gtcatttgcc
    36361 agcacaattt acagagactg aaatgctagt acaatatgat tatttagttg aagatttatt
    36421 aaaaagttta ggcatcccat atgttcgaga agaccgtaaa gttaataagg ctttcagaca
    36481 tatagggcat tctcatgatt gatcatacac acttaagatt atttcagttc tgtgattcac
    36541 agtttccaac aggtgctttc agtcattcat ttggtcttga aacatatatt caacgaaata
    36601 taattcatga cgatcatact tttattgctt ggttaaaaat gtttttacaa gagcaactta
    36661 cctattctga tggtttagcc atgcgtttag tttatgatgc attggaaaat gatgatacac
    36721 aaaaagtatt acacatagat aaactgatgt ttgttcaaaa cttacctaaa gaaacacgtg
    36781 ttggtgcaaa acaaatgggg actcgcatgg ttaaattggc tttagaactt tataatagcc
    36841 catggattgc ttggtatcat caacaaatgc aagataagaa agcaaagtta aatccagcta
    36901 tttgttttac tatgctaggc catcatttag gtgtagatat tgaaacgatt attgattact
    36961 atttatatca aaatgtttca agtttaactc aaaatgctgt tcgtgctatt ccacttggac
    37021 aaactgctgg tcaaaagata gttactcaca tgattcctta tatagaagga acaagaaagc
    37081 agattttcga attaaaagaa gcagattttg gcatgacagc gcctggttta gaactaaatc
    37141 aaatggcgca tgagaacgtc aatgttagaa ttttcatatc ataggaggtt ataattgtgg
    37201 caaatccgat taaaattggt attggtggtc ctgtaggtgc aggtaaaaca caattaattg
    37261 aaaaagttgt aaaacgtctt tcaaaagaaa tgagtatcgg cgttattaca aatgatatat
    37321 atacaaaaga agacgaaaag atattagtaa attcaggagt tctacctgaa agtcgtatca
    37381 ttggtgttga aactggtgga tgtcctcata ctgcgattcg tgaagatgca tctatgaact
    37441 ttgcagcaat agacgaatta ttagaacgtc atgacgatat agaactaatt ttcatagaat
    37501 ctggtggcga taacttagca gcaacattta gcccagaact tgttgacttt tcaatatata
    37561 ttatcgatgt tgctcaaggt gaaaagattc cacgtaaagg tggtcaaggt atgattaagt
    37621 cagatttctt tataattaac aaaactgatt tagctcccta tgtaggtgca tcattagaac
    37681 aaatggctga agatactaaa gtatttcgtg gtaaacgtcc atttactttt actaacttaa
    37741 aaactgacga aggtttggat gaagttatcg attggattga acgcgacact ttactcaaag
    37801 gattatcata atggatgaac aacaatggac tgggcaactt gatttaacag tgttttttga
    37861 tggcaaccga tcagtatcaa gagatatttt ctttgaaaaa gcacttaaag tgatacgtcc
    37921 agtttatcta aatcaatcta ccattcctac attttatata gtaaatgtag gtggtggtta
    37981 tttagatgga gatcgttacc gtatgaatgt gaatgtcgaa gataacgcta aagtgacatt
    38041 gacatctcaa ggtgcaacaa aaatatacaa gacaccttcc aatcatgttg agcagtatca
    38101 aacttttaat ttgaaagata acgcatattt agaatatgtc gctgatccaa tcatcgcata
    38161 tgaaaatgct aaattttatc aacacaatac gttcaatctc aataattcta gttcattatt
    38221 ttatactgat attttaactc ctggttattc aaaaactggc gaagccttca agtatcaata
    38281 tatgcattta ataaatgaaa tttatattga agatgagtta gtcacatatg ataatttatt
    38341 attgaatcct aataaacaat cgatcaatga aataggttat atggaacatt actctcatta
    38401 cggctctgct tacttcatac atgaagatgt taaccaaaaa ctaatcgatt cagtttatga
    38461 aaccattagt tcttatagca atacattcga ttgtcgtgtt gctatttctc aattgcctac
    38521 acatggtttt gcagttagaa tttttgctta tcgcacacaa attatagaga aaatacttgg
    38581 aatcatccaa agctatatcg cagaaaatat ttatgatcgt aaacttgatt ttctaagaaa
    38641 atattaaaat aaaaagacta gtgtaccttg tttcaagctt aataagctga aataaggtat
    38701 ggctagtctt taactttaat taaagcatct tcaatattta aattttaagt taattttatg
    38761 tcccaatgct gaataaataa cttcgtttta ataaattgtt atcgttcaat gttattaaac
    38821 gcaattaacc ttgatttaat ttttaatgta ttcttctaat tctgaaatca gtttttgaat
    38881 atttgctttt tgtgtatctg taacataaac aataactgtt ctttcgtctt gtaaacttct
    38941 tttctttgat aacaatttta aatcttttag cttttgtaaa gctttagtta aatagtaagg
    39001 tttgaactct gagcacttag caatctcttt agatgagatt tcgttagact cacttcttaa
    39061 aatatgattt aaaatataaa tttcttcata gttcaaattg aacttctttt ttgtatctct
    39121 gaaaaacttc ttaacttgaa atgttgcgtt gactaaatca ttaatgtcat taattttact
    39181 cattattaaa cca
//
LOCUS       DBNENW010000006        37775 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNENW010000006 DBNENW010000000
VERSION     DBNENW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.006"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            151..1818
                     /locus_tag="AC1LW8_000251"
     CDS             151..1818
                     /locus_tag="AC1LW8_000251"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIP4772121.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(2064..2153)
                     /locus_tag="AC1LW8_000252"
     CDS             complement(2064..2153)
                     /locus_tag="AC1LW8_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772122.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            2152..4839
                     /gene="harA"
                     /locus_tag="AC1LW8_000253"
     CDS             2152..4839
                     /gene="harA"
                     /locus_tag="AC1LW8_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HIP4772123.1"
                     /translation="MNKHHPKLRSFYSIRKSILGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKTYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTINNANNQPQATTN
                     ISQPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKFPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDVQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPDSDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKGADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HNGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            4970..5062
                     /locus_tag="AC1LW8_000254"
     CDS             4970..5062
                     /locus_tag="AC1LW8_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772124.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(5042..5947)
                     /locus_tag="AC1LW8_000255"
     CDS             complement(5042..5947)
                     /locus_tag="AC1LW8_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIP4772125.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            6329..7591
                     /gene="tyrS"
                     /locus_tag="AC1LW8_000256"
     CDS             6329..7591
                     /gene="tyrS"
                     /locus_tag="AC1LW8_000256"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIP4772126.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(7685..8959)
                     /locus_tag="AC1LW8_000257"
     CDS             complement(7685..8959)
                     /locus_tag="AC1LW8_000257"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIP4772127.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            9131..9748
                     /locus_tag="AC1LW8_000258"
     CDS             9131..9748
                     /locus_tag="AC1LW8_000258"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIP4772128.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            9876..11342
                     /gene="nagE"
                     /locus_tag="AC1LW8_000259"
     CDS             9876..11342
                     /gene="nagE"
                     /locus_tag="AC1LW8_000259"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HIP4772129.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSEIELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            11449..12579
                     /locus_tag="AC1LW8_000260"
     CDS             11449..12579
                     /locus_tag="AC1LW8_000260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4772130.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(12540..12635)
                     /locus_tag="AC1LW8_000261"
     CDS             complement(12540..12635)
                     /locus_tag="AC1LW8_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772131.1"
                     /translation="MKPLVQSFTSSCAVKQQSNYKLSSTPLSSPK"
     gene            complement(12729..14333)
                     /gene="serA"
                     /locus_tag="AC1LW8_000262"
     CDS             complement(12729..14333)
                     /gene="serA"
                     /locus_tag="AC1LW8_000262"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIP4772132.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(14320..15480)
                     /locus_tag="AC1LW8_000263"
     CDS             complement(14320..15480)
                     /locus_tag="AC1LW8_000263"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIP4772133.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(15594..16040)
                     /locus_tag="AC1LW8_000264"
     CDS             complement(15594..16040)
                     /locus_tag="AC1LW8_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIP4772134.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGLGT
                     NPDELLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            16120..16863
                     /locus_tag="AC1LW8_000265"
     CDS             16120..16863
                     /locus_tag="AC1LW8_000265"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIP4772135.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRISDYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(17079..17681)
                     /gene="rpsD"
                     /locus_tag="AC1LW8_000266"
     CDS             complement(17079..17681)
                     /gene="rpsD"
                     /locus_tag="AC1LW8_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIP4772136.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(17925..18389)
                     /locus_tag="AC1LW8_000267"
     CDS             complement(17925..18389)
                     /locus_tag="AC1LW8_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIP4772137.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            18526..20220
                     /gene="ezrA"
                     /locus_tag="AC1LW8_000268"
     CDS             18526..20220
                     /gene="ezrA"
                     /locus_tag="AC1LW8_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIP4772138.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(20294..20434)
                     /locus_tag="AC1LW8_000269"
     CDS             complement(20294..20434)
                     /locus_tag="AC1LW8_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772139.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            20583..21731
                     /locus_tag="AC1LW8_000270"
     CDS             20583..21731
                     /locus_tag="AC1LW8_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP4772140.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTVLNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            21731..22954
                     /gene="thiI"
                     /locus_tag="AC1LW8_000271"
     CDS             21731..22954
                     /gene="thiI"
                     /locus_tag="AC1LW8_000271"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIP4772141.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAILKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            22998..23768
                     /locus_tag="AC1LW8_000272"
     CDS             22998..23768
                     /locus_tag="AC1LW8_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIP4772142.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMATSMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            23866..24360
                     /gene="tpx"
                     /locus_tag="AC1LW8_000273"
     CDS             23866..24360
                     /gene="tpx"
                     /locus_tag="AC1LW8_000273"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIP4772143.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            24483..25430
                     /locus_tag="AC1LW8_000274"
     CDS             24483..25430
                     /locus_tag="AC1LW8_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4772144.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHYLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            25518..26720
                     /locus_tag="AC1LW8_000275"
     CDS             25518..26720
                     /locus_tag="AC1LW8_000275"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIP4772145.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(26966..27466)
                     /locus_tag="AC1LW8_000276"
     CDS             complement(26966..27466)
                     /locus_tag="AC1LW8_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIP4772146.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            27607..28725
                     /gene="ald"
                     /locus_tag="AC1LW8_000277"
     CDS             27607..28725
                     /gene="ald"
                     /locus_tag="AC1LW8_000277"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIP4772147.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            28849..28947
                     /locus_tag="AC1LW8_000278"
     CDS             28849..28947
                     /locus_tag="AC1LW8_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772148.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(29041..30096)
                     /locus_tag="AC1LW8_000279"
     CDS             complement(29041..30096)
                     /locus_tag="AC1LW8_000279"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP4772149.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            30271..30960
                     /locus_tag="AC1LW8_000280"
     CDS             30271..30960
                     /locus_tag="AC1LW8_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIP4772150.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(31265..31678)
                     /locus_tag="AC1LW8_000281"
     CDS             complement(31265..31678)
                     /locus_tag="AC1LW8_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIP4772151.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            31883..33181
                     /locus_tag="AC1LW8_000282"
     CDS             31883..33181
                     /locus_tag="AC1LW8_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIP4772152.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRDTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            33635..34576
                     /gene="pde2"
                     /locus_tag="AC1LW8_000283"
     CDS             33635..34576
                     /gene="pde2"
                     /locus_tag="AC1LW8_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HIP4772153.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSAGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            34597..>37775
                     /locus_tag="AC1LW8_000284"
     CDS             34597..>37775
                     /locus_tag="AC1LW8_000284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIP4772154.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNP"
BASE COUNT        13079 a         5492 c         6884 g        12320 t
ORIGIN      
        1 taagtgaatt tattgcaaaa atgaaagcgc taacccgatt tagtcgacaa gtttttaaca
       61 gttcgttatt atatgaatgt aagtaaaaat ttcttagcta caacttacat attataaatg
      121 cataaattaa acaaaaaggg gcgaaaaaag ttgactcatt tatcagattt agatattgcg
      181 aatcaatcaa cactacaacc aattaaggat attgctgcat cagtaggtat ttcagaggat
      241 gcattagaac cttatggtca ttacaaagct aaaatcgaca ttaataaaat tacgccaaga
      301 gaaaacaaag ggaaagttgt tttagtaact gcgatgagcc caacaccagc tggtgaaggt
      361 aaatcaacgg ttacagttgg tttagctgat gcattccatg agttaaataa aaacgttatg
      421 gttgcattaa gagagcctgc tttaggacca acatttggta ttaaaggtgg tgcgactggt
      481 ggtggttatg cgcaagtctt acctatggaa gatatcaact tacattttaa tggggatttc
      541 catgcgatta caactgctaa taatgcattg tctgcgttta tagataatca tattcaccaa
      601 ggtaatgaat taggaatcga tcaaagacgt attgaatgga aacgtgtatt agatatgaat
      661 gatcgtgcac ttagacatgt aaatgttggg ttaggtggac ctacaaatgg tgtaccacgt
      721 gaagatggct ttaatattac agtagcgtct gaaattatgg cgattttatg tttaagtaga
      781 agtattaaag acttaaaaga taaaattagt cgtattacta ttggttatac tagagatcgc
      841 aagccagtta cagttgcaga tttaaaagtg caaggtgcac ttgcaatgat tttaaaagat
      901 gcaattaaac caaacttagt acaatcaatt gaagggacac cagcattagt tcatggtgga
      961 ccatttgcga atatcgcaca cggttgtaac tcaattttag caactgaaac agcacgtgat
     1021 ttagctgata tcgttgtaac ggaagctgga tttggttcag acttaggcgc tgaaaaattc
     1081 atggacatta aagcgcgtga agcaggattt gatccggcag ctgtcgttgt tgttgcgaca
     1141 attcgtgcgt taaaaatgca tggtggtgta gcgaaagata atttaaaaga agaaaatgta
     1201 gaagcagtaa aagcaggaat tgttaattta gagcgtcatg ttaataatat taaaaaattc
     1261 ggtgtagaac cggttgttgc aattaatgca tttatacatg ataccgatgc agaagtagaa
     1321 tatgtaaaat cttgggctaa agaaaataac gtacgaattg ccttaactga agtttgggaa
     1381 aaaggtggta aaggtggcgt tgacttagca aatgaagtat tagaagtcat tgatcaacct
     1441 aattcattta aacctttata tgaattagaa ttaccattag agcaaaagat tgaaaagatt
     1501 gtgactgaaa tctatggcgg ttcaaaagta acgtttagca gtaaagcgca aaaacaatta
     1561 aaacaattta aagaaaatgg ttgggataat tacccagtat gtatggcgaa aacacaatat
     1621 tcattctcag atgatcaaac gttgttaggt gcaccatcag gatttgaaat tacaattcgt
     1681 gaattagaag cgaaaacagg tgcaggattt atcgtagcgt tgacaggtgc aatcatgact
     1741 atgcctggtt tacctaaaaa accagcagca ttaaacatgg atgttactga tgatggtcat
     1801 gcaattgggt tattctaata aatcatgtca attgtttaat aaagataagt aaatagttta
     1861 atagaccgga ctgttggaga tgcattattt cagcagttcg gttttttgct gtgctaaaaa
     1921 tagattcaat ttggcgaatc taacgacaat gtttgaaggt ggttaattaa tgtatatgaa
     1981 gataaaaagt gggcttgaag aataggaaag cgatgcaatg aatattccat attaaaaaaa
     2041 aataataaaa taggttgcaa tatttaattg ggatgcgcta caattaacac taataattga
     2101 tattgataat tattatcaat taaatataat cttataggag ttgttaacaa catgaacaaa
     2161 catcacccaa aattaaggtc tttctattct attagaaaat caattctagg cgttgcatcg
     2221 gtcattgtca gtacactatt tttaattact tctcaacatc aagcacaagc agcagaaaat
     2281 acaaatactt cagataaaat ctcggaaaat caaaataata atgcaactac aactcagcca
     2341 ccaaaggata caaatcaaac acaacctgct acgcaaccag caaacactgc gaaaacctat
     2401 cctgcagcgg atgaatcact taaagatgca attaaagatc ctgcattaga aaataaagaa
     2461 catgatatag gtccaagaga acaagtcaat ttccaattat tagataaaaa caatgaaacg
     2521 caatactatc actttttcag catcaaagat ccagcagatg tgtattacac taaaaagaaa
     2581 gcagaagttg aattagacat caatactgct tcaacatgga aaaagtttga agtctatgaa
     2641 aacaatcaaa aattaccagt gagacttgta tcatatagtc ctgtaccaga agaccatgcc
     2701 tatattcgat tcccagtttc agatggcaca caagaattga aaattgtttc ttcgactcaa
     2761 attgatgatg gagaagaaac aaattatgat tatactaaat tagtatttgc taaacctatt
     2821 tataacgatc cttcacttgt gaaatcagat acaaatgatg cagtagtaac gaatgatcaa
     2881 tcaagttcag acgcaagtaa tcaaacaaac acgaatacat ctaatcaaaa tacatcaacg
     2941 atcaacaatg ctaataatca accgcaggca acgacaaata ttagtcaacc tgcacaacca
     3001 aaatcgtcag cgaatgcaga tcaagcgtca agccaaccag ctcatgaaac aaattctaat
     3061 ggtaatacta acgataaaac gaatgagtca agtaatcagt cggatgttaa tcaacagtat
     3121 ccaccagcag atgaatcact acaagatgca attaaaaacc cggctatcat cgataaagaa
     3181 catacagctg ataattggcg accaattgat tttcaaatga aaaatgataa aggtgaaaga
     3241 cagttctatc attatgctag tactgttgaa ccagcaactg tcatttttac aaaaacagga
     3301 ccaataattg aattaggttt aaagactgct tcaacatgga agaaatttga agtttatgaa
     3361 ggtgacaaaa agttaccagt cgaattagta tcatatgatt ctgataaaga ttatgcctat
     3421 attcgtttcc cagtatctaa tggtacgaga gaagttaaaa ttgtgtcatc tattgaatat
     3481 ggtgagaaca tccatgaaga ctatgattat acgctaatgg tctttgcaca gcctattact
     3541 aataacccag acgactatgt ggatgaggaa acatacaatt tacaaaaatt attagctccg
     3601 tatcacaaag ctaaaacgtt agaaagacaa gtttatgaat tagaaaaatt acaagagaaa
     3661 tttccggaaa aatataaggc ggaatataaa aagaaattag atcaaactag agtagagtta
     3721 gctgatcagg ttaaatcagc agtgacggaa tttgaaaatg ttacacctac aaatgatcaa
     3781 ttaacagatg tacaagaagc gcattttgtt gtttttgaaa gtgaagaaaa tagtgagtca
     3841 gttatggacg gctttgttga acatccattc tatacagcaa ctttaaatgg tcaaaaatat
     3901 gtagtgatga aaacaaagga tgacagttac tggaaagatt taattgtaga aggtaaacgt
     3961 gtcactactg tttctaaaga tcctaaaaat aattctagaa cgttgatttt cccatatata
     4021 cctgacaaag cagtttacaa tgcgattgtt aaagtagttg tggcaaacat tggttatgaa
     4081 ggtcaatatc atgtcagaat tataaatcag gatatcaata caaaagatga tgatacatca
     4141 caaaataaca cgagtgaacc gctaaatgta caaacaggac aagaaggtaa ggttgctgat
     4201 acagatgtag ctgaaaatag cagcactgca acaaatccta aagatgcgtc tgataaagca
     4261 gatgtgatag aaccagattc tgacgtggtt aaagatgctg ataataatat tgataaagat
     4321 gtgcaacatg atgttgatca tttatccgat atgtcggata ataatcactt cgataagtat
     4381 gatttaaaag aaatggatac tcaaattgcc aaagatactg atagaaatgt ggataaaggt
     4441 gccgataata gcgttggtat gtcatcgaat gtcgatactg ataaagactc taataaaaat
     4501 aaagacaaag tcatacagct gaatcatatt gccgataaaa ataatcataa tggaaaagca
     4561 gcaaagcttg acgtagtgaa acaaaattat aataatacag acaaagttac tgacaaaaaa
     4621 acaactgaac atctgccgag tgatattcat aaaactgtag ataaaacagt gaaaacaaaa
     4681 gaaaaagccg gcacaccatc gaaagaaaac aaacttagtc aatctaaaat gctaccaaaa
     4741 actggagaaa caacttcaag ccaatcatgg tggggcttat atgcgttatt aggtatgtta
     4801 gctttattca ttcctaaatt cagaaaagaa tctaaataat taactggata tagcatatgt
     4861 atgattaact ttgtagacaa tgtgaaacca attaaattat aaactattga ttggtttaat
     4921 ggctttcctt tagagtaaat aaaaagaaca gcagtgagaa attttctaat tgaaaataat
     4981 cttactgctg tttttaatat ttggattcat tgttgtggtt actttaaaaa gtgagcatca
     5041 attaacgctt ttttcgattt aacaaatgtg atttaatatc atattttaat gcgtcgttgt
     5101 attctttttc agtgatttga tcttcgatta acatacgctt taatacataa tgttgtcttt
     5161 ggatactata tttcaaatct ttatccgatt ttaatgttcc atctttttcg tagggtgtat
     5221 agccataagg gctttgcaac aaaccgataa ggtatgcaga ttgtgcaatt gataaatctt
     5281 ttggtggaat accaaacaaa ctatatgaag cggatgcaat tccggaaata ttagcgccat
     5341 tataatctct accgaaggga actatattta aatatgtata tataatttca tcttttgaga
     5401 gtaggtgttc taatctaatt gctaggcgaa gttcatttgc ttttctacta tatgtttttt
     5461 cgttggtaag aacttgattt ttaacaagtt gttgtgtaat tgtgctacca cctgaacttt
     5521 gatcagtatt aaaaatatct tgtatcattg ctcttaaaat cgcctttggt aagatgccat
     5581 catgtttata aaataaagtg tcttcagatg acgttaatgc tttaatgaca tttggacttg
     5641 atgttttagg gcctataatg agtgagtttt gagaatggtt atactcatat aataaatttt
     5701 tgttattatg atctaataat tcatcgccag gtatttgtcg aacttttttt attaaagcat
     5761 catctgataa tgagtcggac gttttagtta aatgatgaaa atataaagac atcgcaatca
     5821 cagcgatagc aacgatggca atgaaaatca taaatataaa aacaatgaca tgtttgatag
     5881 ctttatatat cttttcaaaa tgatatatac gatgattcaa ttgatgattg ttgtcttgat
     5941 tcgtcatctt taacctccta aattacgtat agtataccat atttaagcaa tttgactttt
     6001 attttaacgt taattataat tagggaaaat gcataaagaa taggataaaa ggacaagtaa
     6061 tattatttga aatcatcaga gagctagtgg tcgctgtgaa ctagtgtttt taataatatg
     6121 aatacacctt tataactaac atgtcctacg tttattaatg aacgtatcat taatagagtg
     6181 aacattatct tatgattatg tttaagtttg gtggtaacgt gcaatcgcgc ccttacatat
     6241 ttgttgtaag ggtttttttt acgttcaaaa aactttaaag tattttaaca agtattcaat
     6301 aaataattat aaataggagg aaataataat gacgaatgta ttaattgaag atttaaaatg
     6361 gagaggtctt atttatcaac aaactgatga acaaggtatt gaagatttat taaataaaga
     6421 acaagtgacg ttatactgcg gtgccgatcc aacggcagat agtttacata ttggtcactt
     6481 actaccattc ttaacattaa gacgttttca agaacatgga catcgtccta tcgttttaat
     6541 tggcggtggt acaggtatga ttggtgatcc atcaggtaaa tcagaagaac gtgtgctaca
     6601 aacagaagaa caagtagata aaaatatcga aggtattagt aagcaaatgc acaatatttt
     6661 tgaatttgga acagaccatg gtgcagtgct tgttaataat agagactggt taggacaaat
     6721 ctcattaatt agctttttac gtgactatgg taaacacgtc ggcgttaatt acatgttagg
     6781 taaagattca atccaaagtc gtttagaaca tggtatttca tatacagaat tcacatacac
     6841 gattttacaa gctattgatt tcggtcattt gaatagagaa ttgaattgta agattcaagt
     6901 aggtggatca gatcaatggg gtaatatcac aagtggtatt gaattaatgc gtcgtatgta
     6961 tggtcaaaca gacgcatacg gtttaactat tccgcttgta actaaatcag atggtaagaa
     7021 atttggtaag tctgagtcag gtgctgtttg gttagatgct gaaaaaacaa gtccttatga
     7081 attttatcaa ttctggatta atcaatcaga cgaagatgta attaaattct taaaatactt
     7141 tactttctta ggaaaagaag aaattgatcg cttagaacaa tctaaaaatg aagcaccgca
     7201 tttacgtgaa gctcaaaaaa cattagctga agaagtaact aaatttattc atggtgaaga
     7261 tgcattaaat gatgcaatcc gtatttcaca agcattattt agtggtgatt taaaatcatt
     7321 atcagcgaaa gaattaaaag atggatttaa agatgtgcct caagtgacat tatcaaatga
     7381 cacaacaaat atcgttgaag tccttattga aacaggcatt tctccttcta aacgacaagc
     7441 acgtgaagat gttaacaatg gtgcgattta tattaatggt gagagacaac aagatgttaa
     7501 ttatgcttta gcaccagaag ataaaattga tggcgaattt acgattattc gtcgcggtaa
     7561 gaaaaaatac ttcatggtta actatcaata aatataattg catagctaaa taaattagag
     7621 cctactcata ttcattccta agaatgtaat gagtaggctc ttaatgtact tttctgtctg
     7681 taaattatct aaagaaatct ctatcgcctt ggccaggtga ttgacgttct gatcggcttt
     7741 gacgttttgg ttgttctttt tgttgtttta atttcacttt aacttctttt gttttaccat
     7801 cacggataac ggtaacagtg actgattcac caggtttttt attttcatat aaatagcttc
     7861 ttaaatcaac atcatcttta attttcttgc catcaatttc tgtaataata tcaccttttt
     7921 taagatcaat atcactatca gctttggcga cataaatacc gtcttctcta tcagtatgaa
     7981 gttgctcgcg ctcttcttca ggaatatctt tcaaattaat taaaccaata ccaatcgaag
     8041 ggcggtcaat tttaccatgt tttacaagtt gttcaattgt tactttaact tcattacttg
     8101 gaatagcaaa cccgatacct tcaacttgtg tcgcagcaat tttcattgag ttaataccaa
     8161 ctaaattacc attaatatct actaatgcgc cacctgagtt acctgggtta atagcagcat
     8221 ctgtttgaag aacgctgact tttgtattgc caccagttgt ctcagcgtca atagtacgtt
     8281 cgcttgctga aatgatacca gatgttacag agttagcaaa ttgtaatcct aatgggttac
     8341 ccattgcgaa tacgctatcg ccagtttgta cttttgaaga gttggcgaat tgaatcgctt
     8401 taataccttt tgtattttca attttaagta cagcaatatc agttactgca tctttaccaa
     8461 ctaatttcgc tttaacttgt tttttattat gtaattggac tctaatttca tttgcgccat
     8521 caataacatg attgtttgta acgatataag ctgaattgtt gtttatttga tagataacac
     8581 ctgaacctac tccagcttca gatggtttag atgatttgcc ttttaataag tcgtctacac
     8641 ttgatgcttt ttgcatgtta ataactccaa caattgtagg ggagacagat tttatcattt
     8701 catgaacggt accgaatttc ttgctttgac cgtctaattg attgccacct ttattatttg
     8761 ttgtctgaac agttgaacca tctttattta aaattgtact atttaatact ttgcctatac
     8821 caagtactag aagtgcacca ataattccag caatcaatgc aacgatgact gttttaaacc
     8881 atggaaattt aggtcttctg tatctaggtg tttggctatg gtttgttgta gaatgatctg
     8941 tatgattaaa atctgacata cttaacctcc attatatgat ttatatatgc tttaattatg
     9001 tctttttttt ataattattg ctagtaatac actatacaac tttagatgta ttttcagact
     9061 atcttgttct agtaaaaata tagtgataaa atctagttgt tataaacaaa taagttaagt
     9121 aggtggcaca atgtattcag tgattagtaa gattttgaat ttcattttag ttaaaatgtc
     9181 aaaatcatta tacgtaatcg gtaaagataa tattcctaag gatagtaaat atgtcgtaac
     9241 ttgtacgcat gaaagttata acgaagtcat tatgctaggt atggcattat atcctaatca
     9301 aattcattat atggctaaaa aagaattatt caaaaataaa tggattggta aatttttaac
     9361 gtctttaaat gcatttcctg tagatcgtga aaatccagga cctagtactt taaaacgccc
     9421 aatcaatttg ttgaaagata ataagacagt tggtatcttc ccaacaggtt ctcgcacatc
     9481 gcaagaaggt gcacctttga aacgtggtgc ttcaacaatt gcaatgttaa gtaagtcacc
     9541 aatattacct gttgcttatg ttggtcctac taagattcac ggtcttttaa caggacaagc
     9601 atacataaat attggtaaac caatcgatat taacgattta cctaaagatt taaaacgaaa
     9661 cgagcgtatc gattatatta caaaagagat tgaaacacga acagcaaagt tacaacaaga
     9721 attgcatgaa attgtaaaaa gtttataaaa taacttgagg tagaaatata gctggttagt
     9781 aagcctaggt gactataacg gagtatcgaa gtagttattc tagttacatt agtaatcttt
     9841 atatttctac cttattttaa gaaggatgac attcaatgta taaatttttc caaaatttag
     9901 ggcgttcgtt aatgttacca gttgccattt taccggctgc agcaattatt gccggaattg
     9961 gtaatacgtt aaatgcactt catgcgacgc cgaaaatcgc aatgttcttt actacggtag
    10021 gtactacgat tttagagcaa ttaggtattt tatttgcaat tggtgtagct ataggaatgg
    10081 caaagaaaaa tgatggtgca gtcgcgcttg cagctacgtt aggatatttt ttagttacag
    10141 ttgtattatc accaatgaaa ttagcgcctt tattaggaat gaaagcttcg gaaataaatt
    10201 cagcttttga aaagatgaat aatggcaatg tctttgttgg tattgtaata ggtttaatag
    10261 cagcttatgc atacaataag tttagtgaaa tagaattacc attagcatta tcatttttta
    10321 gtggtaaacg tctagttcca attatgactg cattttactg tacattttta gttgtcatat
    10381 tgttattctt atggccacta ctttattcat ggattgtaaa atttggtgaa tctatagttg
    10441 gtttaggatc gtttggtgct ttcatatatg gtgttgctaa cagactatta attcctacag
    10501 gattacatca tgcactgaat agtgtgttct ggtttgatac gattggtatt aatgatattg
    10561 gtaaattcca aagtggcaaa gatgctataa aaggtattac aggacgctat caagcgggat
    10621 tcttcccaat catgatgttc ggtattccag cggctgcgtt agcaatgtat catacagcta
    10681 aaacgactca gaaaaaacaa gtttacggtt ggttcctagc gagttcagtt gcagcatttt
    10741 tcgttggtgt tacagagccg attgagtttg catttatgtt tgttgcgcca atcttatatg
    10801 ttgttcatgc gttattaaca ggattatctt tatttatcgc agctacattc cattggacag
    10861 ctggattttc atttagtgca ggtttgatag attatgtgtt atcactaatt aatccggtat
    10921 caaatcatcc attgatgttg cttgttcaag gtgttgtatt tttcatctta tactatgtga
    10981 ttttccgtgt agtaatccaa gtatttaact tgaatacgat tggtagaggt gaaaatgaat
    11041 tagttgatcc aacagttgta aaagataata ttgctcctgg tgaaaatgat attaaacaaa
    11101 gtaaatatca tcaacatgct atacaaatat tagaaggttt aggtggtcaa gagaatattg
    11161 ttaatttaac taattgtgca acaaggttgc gtctagagtt aaaagacaca tctattattg
    11221 atcaacagaa gattaaaaac gccggtgcag ttggtgttac tgttaatggt aaacattcaa
    11281 ctcaagttat tgtcggtaca cacgtacagc aggttgctga tgaaattgaa aaacatttat
    11341 aaaatttaaa cagctaaagc gcaaatccaa cttcaatagg gtttgcgctt tttttagtca
    11401 aatagtagaa tgatttatat ctgaattttc agaaatggag tggggattgt gaagaaaatt
    11461 ttatttgatg ttgatggcgt ttttttaagt gaagaacgtt gtttcgatgt ctctgcatta
    11521 acggtatatg aattattaat ggataagtgt tatttaggtc ttcattcaca tatagattgg
    11581 gaaactttga ctgataacga tatacaagac attaggaatc gaatttttca aaaagataag
    11641 attttaaaca aattaaaatc gctagggcta aattcaaatt gggatatgtt atttatcgtt
    11701 tttagtattc atttaattga tattttgaaa aagttatcac atgatgaaat tgaggcattc
    11761 atgtatcaag atgagcctgt cgaattgaag ttgcaaaata taagcacaaa cttagcagac
    11821 tgttttaatt taaatgaaca actaccttta caatttttgg ataatgtaaa agttggtaaa
    11881 aataatattt atgctgcttt ggaagagttt gcaacaacag agttacatgt ttcggatgct
    11941 actttattta gtttgaaggg tgcattatgg acgttagcgc aagaagttta tcaagaatgg
    12001 tatttaggat cgaagttgta tgaagatgtt gaaaagaaaa tagcacgaac tacttttaag
    12061 acaggttata tttatcaaga aattattttg agaccagtag atgaagttaa ggtacttctg
    12121 aatgatttaa aaggtgctgg tttcgaatta ggtattgcaa caggtcgtcc ttatactgag
    12181 actgttgtgc catttgaaaa tttaggattg ttaccatatt ttgaagctga ttttattgca
    12241 acagcaagtg atgttttaga agcagagaat atgtatccgc aagcacgacc attaggaaag
    12301 ccgaatcctt ttagttatat cgcagcttta tatggtaata atcgcgataa atatgaatct
    12361 tatatcaata agcaagataa cattgtaaat aaagatgacg tatttatagt aggcgattcg
    12421 ttagctgact tattaagtgc tcaaaaaata ggtgcaacgt ttattggaac attaacaggt
    12481 ttaaaaggta aggatgctgc aggtgagtta gaagcgcatc atgccgacta tgttattaat
    12541 catttaggtg aacttagagg tgtactagat aatttgtaat ttgattgttg tttgacagca
    12601 caacttgaag tgaatgattg aaccaaaggt ttcatattga gttacaatga aattaataat
    12661 gaaaaaatgc caagaagcaa tggaagtaat ccaatgtctt cttggcattt tgaatttaca
    12721 taaattgttt atgactgtac cgtcaattca gttgtgaaaa tttgattgta ttcaccaact
    12781 tgtttaagtt catcaattat attgtttgaa acaggttgat caacggataa aatcattagc
    12841 gcatctccgc ccgcttcagt tctacctaaa gtcatagatg caatgttgat attgtattta
    12901 cctaacaatg cgccagtttt tcctaccata cctggagtat cattatgata tgacacaatt
    12961 tgatattgat ttggctttaa gtctacagaa aaattattaa ttctaacaat tcttggaccg
    13021 aaacctgtaa agacagaagc gccaacttta acggaatcgc tatcgtttga tagttctacc
    13081 tctaagtagt tactaaaacc tgtctctgct ttattatttt caatatttaa tgtcacttgt
    13141 tgttgattta acaacattag agcattgata atattgactt caggaccaag atcatctttt
    13201 aaaatatgag taataattgt acgtgttaat aaactactat caatagaggc taagtcgcca
    13261 ccgtacgtaa ttttaataga gcttggtgca ttgtacatta attgaatagc taattcacca
    13321 actgtttggc ttaaattgat gaatgatttt acagtatcat ctatattgct taagtccatt
    13381 ttaggtgcat tcactgcatg cgttacagta ccatcaatta aaatttcgat gatttcattt
    13441 gaaacagaaa ttgccacttt ttcttgagct tcgactgttg aagcacccaa atgaggtgta
    13501 acaataattt tatcatgtgc aacaagaggc gagtcagttg caggttcatg ttcaaacaca
    13561 tcgatagctg cccgactaat ttgtccttcg tctaatgctt ttattagcgc cttttcatca
    13621 ataataccac cacgtgccac attgattatt tgcaaactag gttttgcttt ggcaaaaaag
    13681 tcagcattaa ttaagccttt tgttttaggt gttagtggtg tatgtaatgt aacgaaatca
    13741 gaatgttggg caatctcatc aactgttgcc ttcgtaatgc ttaaagattt tgctttttca
    13801 tccgttaagt aagggtcaaa agctagtatt ttcattccga aactttgcgc acgtttagca
    13861 acacctaaac caattctacc agcaccaatg acacctaatg ttttatgata aagctcagta
    13921 cctttaaatg catttcgatt ccattcttta tttgtaagtg attggtgtgc ttgcggaata
    13981 tttcgtgcca ttgataataa cattgccagt gtatgttcag tagctgaaat cgtattacca
    14041 tctggggcat taataactaa aatacctttt aatgttgcag cattaatatt aatattatct
    14101 acaccaacac cggcgcgtgc gattactttt aaagaatcag cagcatttat gatattttca
    14161 gtaaccgtag tttgactacg aacgattaaa gcatggtatg aaggtataat tttgattaat
    14221 gcttcttcgg acaagccagt ttgaatatct acattgaatt gttcgtgatc taataatgct
    14281 ttgataccat cttttgatat gggatctgca acgagtacat taaattgctt catgaataac
    14341 ctccatatat tttgatatac ctttaccgat atagttaact ttacggtgtt cagttaaaat
    14401 aatttctaaa gcagatacta ccgataaaat atcgaaagga ctaattttcc ccatatgacc
    14461 aattcttaaa atttgacctt taagatggcc ttgaccacca gcaattgtta ttttaaaccg
    14521 atttttaagt tcatctttga ttattttcac ttcatcattt gtattaggtt tgaatgctgt
    14581 aaccgttgga gatgcatctt tatcattgac taataaagtt aaatctaatg cttttaaggc
    14641 gcttcttaat gcatttctaa ttgcatagtg tcgtgctatt acgtgattga aaccttctgc
    14701 ttttacggtt tcaacgtatg catttacacc tctaaataaa gacacatttg gtgtgaacgg
    14761 tgtagaattg tcagcttgtg acgaaatgta tttatttaag tctagataaa attttggcgt
    14821 agttacttct ttgaaacgtt cttttgcacg gtggctataa gctacaaaag ctaatcctgg
    14881 aggtaacata attgcttttt gactaccaga aacaagtaca tcaattttat ctttgttaat
    14941 gtcaacatca acagcaccaa tgcaacttac gccgtcaact acaaaataaa tattactatt
    15001 aaattgattg atggcatttc ctaactcgtg aatagggtgt agcactgtcg tagatgtttc
    15061 gcaatattga ctaaatactg ctttcacttc aacatttaaa gttgaaagga aattgatgaa
    15121 atctttgaca tctacagctt ctccccatgt tacgtcataa atatgcacat ttttgtaata
    15181 agtttgtgca atttgtttaa atcggttacc aaaggcacct gaaacaatga caacgaagtg
    15241 atcttcaggg tttacaatgt tcaacatact agcctccaag acgcttgtac cgctagatgt
    15301 taaaataagt acatcatttt gactcccaaa tattggcttt agaccttgaa atgcttgttg
    15361 tgcgatgtct tcaaaatctt tagaacgatg accaaccata ggtgcttgaa tttctctcat
    15421 aatggcatca ggtacagggg ttgggccagg tgttaataac aacggttgat gataatacat
    15481 ttatatgcct cctataaata agatttcact attttagcaa attttctgaa aatttaaaag
    15541 cctaaaaaag tgataacgct atattgtcga acaaacgtta tcacttaaag attttatttg
    15601 gcctgaatga tgggataaat ttttatgtcc acattatttc ttacagcatt tgaaatcatg
    15661 caattattat ctgcaattgt tatcaatttt ggtaatcgct tttctaattg tgctatttga
    15721 tcacttggaa tttgaatttg aggatggtgc acaatttttg acatactgaa ttttccgtta
    15781 tttaaacaag ctgttccaat cgattgttgt tcaattgaaa tatctgtgaa ctttgcacgt
    15841 tcaagagtag ctgctaatga gatgatataa catgatgaag cggctgatac taacaattca
    15901 tcgggatttg ttcctaaacc aacaccacct aaggaagcag gtatagaaat attctctgaa
    15961 agtatgtcgc cttgaacgtt tccgacattg ttacgaccac cttgccaaga agtttggact
    16021 ttaaagtcat gttgatgcaa ttgctttaac ctccaatata attgtgatag tttaatttta
    16081 gaatagttta tcaaagttta acagaaaggt gacttatcaa tgactctgaa taaactgaaa
    16141 gatgaattac aaattgtttc gcaccgtgga ttgccgagtg attttcctga aaatacaatg
    16201 gtcggttatc gagaggtaat ggggctcaat gttgctatgt tagaaataga tgttcatttg
    16261 accaaagacc aacattttgt tgtgatacat gatgaaacaa ttgatagaac atcggatggt
    16321 aaggggcgta tttctgatta cacattatcg caattaaaat catttgattt tggtagttat
    16381 aaagatgttg cttttaaggg agaacgaata cccacgttag atgaagtact ttcattatgc
    16441 ttaaagtatg ataaaaagct attgattgaa ttgaaaagtc cgaatttata tccagaaata
    16501 gaatgtaaat tgctggcatt tttggaagaa aagaaagtcg atgcgacaca agtagttata
    16561 caatcttttg atatcgaatg catcgaaaag ttaaacacat tgggcagtat atatgaatta
    16621 ggtgtgcttt gtagtaaacg taattattgg tataaaaagc caaacttttc aagaattgct
    16681 caaattgcta gttatgttaa tcctaattat gcattagtga ccagaaaatt tgttgataaa
    16741 gcacatcatc atcaattaca ggttatgcca tacacagtta ataaattgaa aactggagaa
    16801 aaattaagac aaatgggtgt agatggatta attacagata atccgaaatt atttattaag
    16861 tgagacgatg aatggtaatt tgatagaggg atgaggattc gtatatcgca attgataatt
    16921 ttaaaaacta atgatgaaat actaaagata attaaacaaa taagacttgt aaattctaag
    16981 ttgattgtaa aatagggtag ctccatataa ttttgaatct atcattttta aattaataaa
    17041 aaaaacaccc acaattgtgg gtgtttgtat tgaacgtatt atcttgagta gtactcaacg
    17101 attaattgtt cgttaatttc agcaggtaat tcgctacgtt ctggtaaacg tacgaaagta
    17161 ccagttaagc tgtcagcatc aaagtttaag tactcaggta cgaaattgtt gatttcaact
    17221 gattcaacga tgatgtttaa tttttgagat ttttcacgaa ctgaaattgt ttgaccaggt
    17281 ttaacagaat aagatggaat atcaacacgt ttaccatcta ctaagatatg accgtggtta
    17341 actaattgac gtgcttgacg acgagtacga gctaaaccta atgaataaac aacagcgtct
    17401 aaacgacttg ctaataagat catgaagttt tcaccgtgta caccgaattt tttaccagcg
    17461 atgtcaaatg tgttacggaa ttgtctttca gtcattccat ataagtaacg taatttttgt
    17521 ttttcacgta attgtaaacc atattctgat aattttttac gttggtttgg accatgttgt
    17581 cctggtgcgt aaggacgttt ttctaattct ttaccagtac cgcttaaaga gatacctaaa
    17641 cgacgagatt ttttccagtt tgaacctctg aatcgagcca taataagact cctcctttaa
    17701 ttcttttttg ttgttatgaa taaacaaaaa agagtgttgc atgcttacta agatatattg
    17761 ttttatgtgt cttcacctta tagcgacagt tacgtgacac gtccgcattg ggaacaacat
    17821 agcgctcaat aatatacaac tgctactttc gttaattcac acaaagatta ttgtaacatt
    17881 ttcctgctaa ctgtcaacac tttattgaaa atgctgatga ccttttatgc gagttgcttt
    17941 tcaataattt taacaattgc ttcaagatgt tctttgtcat tgtcatcaaa tcgatcagtt
    18001 ataggggcat cgatatctaa gacgccgata attttatcat ctttgaaaat tggaacgaca
    18061 atctcagact tactattagc atcacaagcg atatgtcctt cgaattgatg gacatcagct
    18121 acaacttgtg tacgacgttc tgaaacggct gtaccacata caccttttcc aattggaatg
    18181 tggacacaag cggggtgtcc ttggaaaggt ccaagtataa gttcattttg ttccaataag
    18241 taaaagccga cccaattaat ttgatctaga ttatcattta ataaggcaga catgttactt
    18301 aaaatagcaa tcatatgatg ttcatcttca ataaggcttg ctgcttgttt ctttaataat
    18361 gtgtagtttg ttgggttaat tgttgtcata tttaaaacct cttattttca tagattttat
    18421 aatagtatag aatttcgaaa atattatggc aataaattca ttttataatt tatccttaag
    18481 caaaattacg ttataatagt aatgataaat taggaggaga agcatatggt gttatatatc
    18541 attttggcaa taattgtgat tatattgatt gctgtaggtg tattattcta tttacgttca
    18601 aataaacgac aaattattga aaaagcaatc gaacgtaaaa atgaaattga gacgttacct
    18661 tttgatcaaa accttgcaca attatctaag ttgaatttaa aaggtgaaac aaaaacgaaa
    18721 tacgatgcaa tgaaaaagga caacgtagaa agtacaaata agtatctagc tcctgtggaa
    18781 gaaaaaatcc ataatgctga ggctttatta gataaattta gtttcaacgc atctcaaagt
    18841 gaaattgatg atgcaaatga gttgatggat agttacgaac aaagctatca gcaacaatta
    18901 gaagatgtaa atgaaattat tgcgttatac aaagataatg atgaattata tgacaaatgt
    18961 aaggttgatt atcgtgaaat gaaacgtgat gttttagcaa atcgtcatca atttggtgag
    19021 gcagcaagtc tacttgaaac tgaaattgaa aagtttgagc caaggttaga gcaatatgaa
    19081 gtactaaaag ctgatggtaa ttatgtacaa gcgcacaacc atatagctgc cttgaatgaa
    19141 caaatgaaac agctaagatc ttatatggaa gaaataccag aattaattag agaaactcaa
    19201 aaagaattac ctggtcaatt ccaagattta aaatatggtt gccgtgatct taaagttgaa
    19261 gggtatgatc tggatcacgt aaaagtagac agtacattac aaagcttaaa aacagagctt
    19321 agtttcgttg aaccattaat tagccgctta gaattagaag aagctaatga taaactagct
    19381 aatatcaatg ataagttaga tgacatgtat gatttaattg aacatgaagt taaagctaaa
    19441 aatgatgtcg aagaaacaaa agatatcatt acggataact tattcaaagc taaagacatg
    19501 aattatacat tgcaaacaga aattgaatat gtacgtgaaa actactatat aaatgaatct
    19561 gatgctcaaa gtgttcgtca atttgaaaat gaaattcaaa gtttaatttc tgtatatgat
    19621 gatattttaa aagaaatgtc taaatctgct gtgcgatata gcgaggttca ggataattta
    19681 caatatttag aagatcatgt cacagttatt aatgacaaac aagaaaagct acaaaatcat
    19741 ctgattcaat tgcgtgaaga tgaagcagaa gcagaagaca atctgctacg agtccaatcg
    19801 aagaaagaag aagtgtatcg tcgattactt gcttctaact taacaagcgt tcctgaaagg
    19861 tttatcatca tgaaaaatga aattgatcat gaagttcgtg atgttaacga acaatttagt
    19921 gaacgtccaa tacacgttaa acagttaaaa gataaagtgt ctaaaattgt gattcaaatg
    19981 aatacatttg aagatgaagc aaatgatgtt cttgttaatg ctgtttatgc agagaaatta
    20041 attcaatatg gaaatagata tcgtaaggac tatagcaatg ttgataagag cttaaatgaa
    20101 gctgaacgat tatttaaaaa taatcgctat aagcgtgcga ttgaaattgc agagcaagct
    20161 cttgaaagtg ttgagccagg tgttactaaa catattgaag aagaagttat taagcaatag
    20221 aaactagtat gtagttatac ttaaataata tgagcactct gtcaaattgg actgatgagt
    20281 ttaataattg aagttagcca acgatacgtt gtctagcttc ttttttatat ggataaatga
    20341 aagggacaat aaatataaat agcaattgtt taaagataaa cgtaatcaaa tgtgttgttt
    20401 taattaatat aagtagtgaa aaaagcataa tcacacagct gtttaaatag agtgaaatag
    20461 tctaattttt atttaataag tagaaataag attattttgc gctataacct ttattttttg
    20521 tcggataata gtataatgct tgagtaaaac aattttaacg attgatgatt aatgaaagag
    20581 aagtgtttat tttgatatat ctagataatg cggcaacgac gaaagcattt gaagaagtgt
    20641 tagatactta tttaaaagta aatcaatcaa tgtattataa tccgaatagt ccgcataaag
    20701 ctggtttgca ggcaaatcaa ttactacaac aagcaaaagc ccaaattaat gcaatgatta
    20761 attcaaaaac aaattatgat gttgtattca ctagtggtgc aactgaatcc aataatcttg
    20821 ctttaaaagg tattgcctat cgtaaatttg atacagcgaa ggaaataatt acatccgtgt
    20881 tagagcatcc gtccgtatta gaggttgtaa gatatttgga agcacacgaa ggatttaaag
    20941 ttaaatatgt tgatgtaaag aaagatggca gtattaactt agaacacttc aaagaattaa
    21001 tgtcagacaa agtcggttta gtaacatgta tgtatgtaaa taatgtaact ggacaaatac
    21061 agcctattcc acaaatggct aaagttataa aaaattatcc taaggcacat tttcatgtag
    21121 atgcggttca agcattcggc aaaatttcaa tggatctcaa taacatagat agtattagtt
    21181 taagtggaca caagtttaat ggtttaaaag gccaaggcgt cttacttgta aatcacattc
    21241 aaaatgttga accaactgtc catggtggtg gtcaagaata tggcgttaga agtggaacag
    21301 ttaatttgcc aaatgatatt gcaatggtta aagcgatgaa gatagctaat gaaaactttg
    21361 aagcattgaa tgcatttgtt actgtgttaa ataatgacgt ccgtcaattt ttaaataaat
    21421 atcatggagt ttatattaat tcttcaactt ctggttcacc attcgtttta aatattagtt
    21481 ttcctggcgt aaaaggtgaa gtattagtta atgctttttc aaaatatgac attatgatat
    21541 ctacgacgag tgcttgttca tctaaacgta ataaattaaa tgaagtattg gctgcaatgg
    21601 gattatcaga caaatctatt gaaggtagta taagattatc atttggggct actacaacta
    21661 aagaagatat agcaaggttt aaagaaatat ttatcatcat ttatgaggaa attaaggagt
    21721 tgctaaaata atgaagtatg atcacttgct tgttagatac ggggagttaa cattaaaggg
    21781 ttcaaataga aaaaaatttg taaatcaatt aagaaataat gtaaataagt cattaaaagg
    21841 acttgatggg tttgtcgtta aaggcaaacg agatcgtatg tatattgaac ttgaagacca
    21901 tgcagatata aatgaaataa catatcgatt atcaaaaatt ttcggtatta aatctattag
    21961 tccagtatta aaagtagaaa aaacaataga ggcaatgagt gcagcggcaa ttaaatttgc
    22021 gcagcaattt gaagaaaaca gcacatttaa aattgatgtg aagcgtgccg ataaaaattt
    22081 cccaatggat acgtatgaat tacagcgtga attgggtggt gcaatattga agcacttcga
    22141 caatatttca gtgaatgtca aacgtccaga tcatgaaatt cgagtggaag ttagattaga
    22201 tgcaatttat atgtatgaag aagttgttcc gggttcaggt ggattaccag ttggtactgg
    22261 tggtaagacg ttactaatgc tttcaggcgg tatagactca ccagttgctg ggatggaagt
    22321 gatgagacgt ggcgtaacaa ttgaagcgat tcatttccat agtccaccat ttacaagtga
    22381 tcaagcaaaa gaaaaagtta ttgaattgac acgtatttta gctgaacgtg ttggaccaat
    22441 taaattgcat attgtaccat ttacagaatt gcaaaaacag gtaaataaag ttgtacatcc
    22501 aagatataca atgacttcaa cgagacgtat gatgatgcgt gttgctgata aattagtaca
    22561 tcaaataggg gctttagcta ttgtaaatgg tgaaaaccta gggcaggtag ccagtcaaac
    22621 acttcatagc atgtatgcaa ttaataatgt aacttctact cctgtattac gtcctttatt
    22681 aacttacgat aaagaagaaa ttattattaa atcgaaagaa attggtacat ttgaaacatc
    22741 tattcaacca tttgaagatt gttgtacaat tttcacccct aaaaatccag taaccgaacc
    22801 aaactttgat aaggtagttc aatatgaaag cgtctttgat tttgaagaga tgattaatcg
    22861 tgctgttgaa aatattgaaa cacttgaaat aactagtgat tataaaacta ttaaagaaca
    22921 gcaaacaaac caattaataa atgacttttt ataaataaaa tcctagagta aatttaaaca
    22981 taaggggatg ttaaactatg gatttgaact taacgatgat tataatcata attttatttg
    23041 gttttatcgc ggcgtttata gattcggttg tagggggtgg cggtttaatt tctacgccag
    23101 cattattagc aatcggtcta ccaccatctg tggctttagg tacaaataaa ttggcaagtt
    23161 catttggttc tttaactagt acgataaagt ttataaggtc cggtaaagtg gacttatatg
    23221 ttgttgccaa attatttggt tttgtatttt tggcatctgc atgtggcgca tatattgcaa
    23281 cgatggttcc gtcacaaata ttgaaacctt taatcattat tgcactttcg tcggtgttta
    23341 tattcacatt acttaaaaaa gattggggca atacacgtac gtttactcaa tttacattta
    23401 agaaagccat actatttgca gcacttttta tattaatcgg cttttatgat ggatttgtag
    23461 gtgggggaac aggttcattt atgctttttg tattgttagt ctttggtttt gattttttaa
    23521 gtgcagcagg aaatgctaaa gttttgaact ttgcttctaa tataggtgcg cttgtattat
    23581 ttatggtatt aggacaagta gattatgtaa taggtttaat aatggctaca agcatgattg
    23641 ctgggtcata tactggggca cattttgcta tcaaacaagg tgttggctat gtaaaagtat
    23701 tatttattat tattactgct attttaattt taaaaaatgc ttttgattat attcaacaat
    23761 ttgtataaaa ttaaatttgc ttttgattaa agcatgaaaa ttgtaatcaa accataaatt
    23821 gtcgtatgat gtagttagaa ttttaaaatg caggaggtca agtatatgac tgaaataaca
    23881 ttcaaaggtg gaccaatcca cttaaaaggt caacaaatta atgaaggtga ttttgcacct
    23941 gattttacag tgttagataa tgacttaaat caagtaacat tagcagatta tgctggtaaa
    24001 aagaaattaa ttagtgtggt accatcaatt gatacaggtg tttgtgatca gcagactcgc
    24061 aaattcaact ctgatgcttc taaagaagag gggattgtgc ttacaatttc agcagactta
    24121 ccattcgcac aaaaaagatg gtgcgcttca gcaggtttag acaatgtcat tacattaagt
    24181 gaccaccgtg acttatcatt tggtgaaaac tatggcgttg ttatggaaga acttcgctta
    24241 ttagctcgtg cagtatttgt attagatgca gataataaag ttgtttataa agaaatcgtt
    24301 agtgaaggta ctgatttccc agattttgat gctgctttag ctgcatacaa aaatatttaa
    24361 tcattaaaga gataaatctt aaaatgtata catcgtgtcc atcgttgtca acagcattaa
    24421 aatagaattg ttttctatga ttgctaagac ctatgggcac tttttattgg agagggacga
    24481 atatggcaga acaacaaaca attatggaac gcttgtttca tacattagat gaaaaagcta
    24541 aaacattaaa taatgaaaat ggccaaagtt ttattgaaaa tcttgggcta gcaatggaac
    24601 aagtatatac caatgaaaga ggattgttag aacaatcgac gttacaagat cgacgtaaag
    24661 cattccaatt tgcatattta agtttaatgc aggaagaaaa gatacaagca aatcatcaaa
    24721 ttacaccaga ttcaattgga ttgatactag gatttttagt tgagcgtttt atgaacaacc
    24781 aagaagaatt acatattgtt gatattgcaa gtggtgccgg tcatttaagt gctactgtaa
    24841 aagaagtgtt acctgaaatt gcggttatgc attatttaat tgaagttgat ccagttttat
    24901 cacgtgttag tgtacattta gcaaacttct tagaaattcc tttcgatgtg tatcctcaag
    24961 atgccatcat gccactacca ttagaagaag cagatatcgt tattggtgat tttccagtag
    25021 gctattatcc aattgatgaa agaagtaagg agtttaagct aggttttgaa gaaggacata
    25081 gttattcaca ttatttatta atagaacaag caataaatgc attaaaagat gctggatatg
    25141 cctttctagt ggtaccaagt aatattttta caggtgaaca tgtaaaacag cttgaaaaat
    25201 atattgcaac agagacagag atgcaagcat ttttaaattt accaccaact ttatttaaaa
    25261 atgaaaaagc gcgaaaatct atattaattt tacaaaagaa aaaatcgggt gaaacaaagc
    25321 cagttgaagt attattggca aatattcctg atttcaaaat tccttcacaa tttcaaggat
    25381 ttatgacaga gttaaatcag tggatggaca caaatcgtcc taaaaaataa tctgtaatag
    25441 tatagtcata aaactgtatg aatgataaaa tgaaaaatga aatagtataa aaagcaaacg
    25501 atggaggatc attacttatg tcaaaattaa tcttggctat caatgctggt agttcatcat
    25561 taaaatttca attaattaga atgcctgaag aggaattagt aacaaaaggt ttagtagaaa
    25621 gaatcggatt gaaggattca atttttacaa ttgaagtcaa cggggaaaaa gttaaaacag
    25681 tacaagatat caaggatcac gttgaggcag ttgatatcat gttggatgct tttaaagcgc
    25741 acaatattat taatgatatt aacgatatcg atggaacagg tcaccgtgtt gtacatggtg
    25801 gtgaaaaatt ccctgaatca gtagctatta ctgatgaggt tgaaaaggaa attgaagaat
    25861 taagcgaatt agcaccgctt cacaacccag ctaacttgat gggtattcgt gctttccgta
    25921 aattattacc aaatatccct catgtggcaa tttttgatac agcattccat caaacaatgc
    25981 ctgaaaaagc atatctatat agcttgccat atcattatta taaagattat ggcattcgta
    26041 agtatggttt ccatggtaca agccataaat ttgtatcaca aagagcggca gaaatgttag
    26101 ataagccaat tgaagattta cgtattattt catgtcacat tggtaatgga gcatcaattg
    26161 cagctatcga tggcggtaaa tctattgata catcaatggg cttcacacca cttgctggtg
    26221 taacaatggg tactcgttct ggtaatattg accctgcttt aattccattt attatggaaa
    26281 aaacaggtaa aactgctgaa caagtattgg aaatcttaaa caaagaatca ggtttattag
    26341 gattatcagg tacatctagt gatttacgtg acttatcaga agaagctgaa tcaggtaaag
    26401 cacgttcaca aatggcttta gatgtatttg cttctaaaat tcacaaatat attggatcat
    26461 atgctgcgag aatgcatggt gttgatgtta tcgtatttac agcaggtatc ggtgaaaact
    26521 cagtagaaat tcgtgcgaaa gttcttgaag gcttagaatt tatgggtgtt tactgggatc
    26581 ctaagaaaaa tgaaaactta ctacgtggta aagaaggatt tattaactat cctcattcac
    26641 cagttaaagt tgtcgttatt ccaacagatg aagaaagtat gattgcccgt gatgttatga
    26701 cattcggtgg tttaaaataa tttttaaata aatattaagc gtctatctcc gctacaatta
    26761 tatgtaggag gtagacgctt tttgtattgt ttgaaaatta actagtgtaa tgtctctttt
    26821 cacgaatatc ttaaagaatt aagtagtcga agaagattat gaaaacatta ttaagtgagt
    26881 tttatttata aacaataaaa aaccaagtgc agaaagttgc acttggttca taatgatcaa
    26941 gtagttatct aatgatcatc tatatttaat tttgatattt ttcacgtaat tgagttgttg
    27001 caacttgtgg ttggaagtct gctggtaact cttcagtacg aacaactaac acgtcacatg
    27061 gcgcatgacg aacgatagat tctgatacag aaccaacaat aaatctttcc acggcattta
    27121 agcctgatgt accactcata atcaagtctg cattaatttc atgtgcaagc tttttaggaa
    27181 tgatagattt aggagagcca aactctagac gcgtttctac atctttaaca ccagcgttag
    27241 tagctacttc tttataacca tttaataatt cttctgcaaa atgcttagat ttttcagtaa
    27301 attgagcatc ataaacttca taagaagaat acgttcttga atcaattaca ttcacaattg
    27361 ttaacttcgc atcgttacgt ttagcaacac caactgctct gttaaatgcc cattccgctt
    27421 catgtgaacc gtcaactgcg attaaaatat ttttgtaagt aatcatagta agtgcctcct
    27481 tgttctttct taattatatt ttaccacaat tttcggaaaa gtcagtgtaa taacctttac
    27541 aattttgtgg atttttgatt gcgctttcaa aatgttcgcg ttaagatgat tatggaggtg
    27601 gatggtatga aaattggtat accaagggag attaaaaata atgaaaatcg tgttggttta
    27661 tcaccaagtg gtgtgcacgc tttagtagaa agtgggcata ctgttttagt ggaaacaaat
    27721 gcgggttcag gatcattctt tgaagatgta gattacaaag aagcaggtgc tgagattgtt
    27781 gctgaacaag caaaagtttg ggatgtggat atggttatta aagttaaaga accacttgaa
    27841 tctgaatatc catattttaa agaagggctt gtattattca cttatcttca tttagcaaat
    27901 gaagaaaaat taacacaagc tttgatagat agaaaagtaa ttagtattgc atatgagact
    27961 gtgcagttac cagaccgatc tttaccattg ttatcaccaa tgagtgaggt agcaggaaga
    28021 atgtcagctc aagttggcgc agagttccta caaaaactta atggtggtat gggaattcta
    28081 ctaggtggtg tcccaggagt acctaagggt aaagtaacta ttatcggtgg tggtcaagca
    28141 ggaacaaatg cagctaaaat tgcactagga ctaggtgcag atgttacgat tttagatgtt
    28201 aatccaaagc gtttacaaca attagatgat ttattcggtg gacgtgtaca tacaattatg
    28261 tcaaatccgt tgaatattga gttgtatgtt aaacaaagtg atttagtaat aggtgcagtt
    28321 ttaattccag gtgctaaagc gccaagactt gtaacagaag acatgattaa acaaatgaaa
    28381 aatgggtcag ttattattga cattgctatt gatcaaggcg gtatttttga aacaactgat
    28441 aaaattacga cacatgatga tcctacatat attaagcatg gtgtggttca ttatgcagtt
    28501 gcaaatatgc caggtgcagt accgcgtact tcgacgttag ctttaaataa tgctacgcta
    28561 ccttatgcgc tcatgctagc taataaaggg tatagagaag catttaaatc aaatcaacca
    28621 ttatcattag gtttaaatac ttacaaaggt cacgtaacca ataaaggcgt tgcagaggca
    28681 tttgaaatgg aatataaatc tgtagaagaa gcattacaat tataatttga atttgatgaa
    28741 aagggaatgg attacagcgt actaaagaat gacgttatca tctccttggt tattaaaatt
    28801 aaataatgat aaattaaagc tagaaacaat gcgaagaaat catcgaacat gaatgatatt
    28861 gatttgattc attacatgtt actgatgaac atacctgcat atgtgtttct agcttgtttg
    28921 ttacatattg catttagact tggataaaaa tttatgtctt aggcgctaca atgttatatt
    28981 ggcagtgttt gactgaatga gaatgcgctt gtaacaagct tttcattttt atcccactcc
    29041 ttatttttcg taatgtgtta atacttcata tccttcatta gtgacaagta tgtcatcttc
    29101 aattcttaca cctgcaacac caggtacata aatacctggt tcgattgtaa taaccatgcc
    29161 agcttctaac aaattagaat tagtacttga aacatcttga tattcatgtt cttgtaatcc
    29221 taggccatga cctaagcgat gagggaaata ttcaccataa cctttttctg aaataatatt
    29281 tctagcgata tgatcgatat cttttaatgg tattccaggt ttaattgctt ggattgcaga
    29341 tgtttctgct tctaatacaa tattataaat ttcttgtgct tctttgctag gttcaccaaa
    29401 tttaatagta cgtgtcatat cgctacaata atgctcataa attacaccta aatcaaatag
    29461 tacatattca ttgcttttta atctgcgatc tcctggtgtg ccatgaggtg atgcggcatg
    29521 atctccaaat aaaaccatcg tatcaaaact catttcattg acgccatatt gtttgatagt
    29581 ttgctcaata tggttgacta cttcacgttc agtcacacct tcttttaaat aagaaacacc
    29641 tatttcgata cacttatctg ctaactcagc agctttacgt attttgctaa tttcatcttc
    29701 ggatttaata tttctcaatt gtttgattgt taaatcaaca tctccgaatg aattgacatt
    29761 gaaaccagag attaattgtt tttggcgtgc tactgttaag tgctcgcttt caattagtaa
    29821 tttattgatt gtttgaggat aaagtgaaaa agggttttca gtgtctaaat atccaacgat
    29881 ttcacctgtg aaaggtgatg ctttgacttc ttcgacttcc atttttggac aaaatagtac
    29941 ttgtttacca tctttcttaa tcaataatgc aaataatctt tcatggggtt cgctacggta
    30001 tccagtaaaa taatatacat tcaacggtgt tgtaatccat gctgcatcag cttgttgatt
    30061 gttcaattcg tctattattt ttgatatttt tgtcatttta taaacactcc tcaacataat
    30121 attacttcca ttttatgcca attattatta gatttcaaaa tcgagctaat acattaacta
    30181 gattttcaat ttttaaaatt tgagaatttg ttataattaa gtggcaaaag ggtactaaat
    30241 ttatatagta attgatagga ggatgctcaa atgaagttat catttcatgg tcaatcaacg
    30301 atttatttag aagggaacaa caaaaaggtt atcgtagatc cgtttatttc aaataaccct
    30361 aagtgtgatt tgaatattga aacagtgcag gtagactata tcgttttaac gcacggtcat
    30421 tttgatcatt ttggtgatgt tgtggaacta gcaaagaaaa caggagcaac agtaattgga
    30481 agtgcagaaa tggctgacta tctttcttca tatcacggtg ttgaaaatgt tcatggtatg
    30541 aatattggtg gtaaagcaaa ctttgatttc ggaagcgtta aatttgttca agcatttcat
    30601 agttcaagtt ttacacatga aaatggtatt ccagtttatt taggtatgcc aatgggtatc
    30661 gtgtttgaag ttgaaggaaa aacaatttat catactggtg atacaggtct gtttagtgac
    30721 atgtcactta ttgcgaaacg tcatccagtt gatgtatgtt ttgtaccaat tggagataat
    30781 tttacgatgg gtattgatga tgcaagttat gctattaatg aatttattaa accaaaaatt
    30841 tctgtaccga ttcattatga tacattccca ttaatcgaac aagatccaca acaatttaaa
    30901 gatgctgtta atgtagggga cgtgcaaatt ttaaaaccag gtgaatctgt tcagttttaa
    30961 atagaatata gattttacac taaaaaacac ctcgctaatc tatgtggaag ttatcgtctg
    31021 catattttta gtgggtgttt ttatattcta ctgattcagt tacgacgtgg acgggggaca
    31081 tcagtggttc tttatcattt ctgacctact ctcgtaattt tagttttata caattagaca
    31141 ttgattataa atttactacg atataaaaaa tccagaacca cagtcataat cgtttcttgt
    31201 atttactcag tcgtttgaga ctgcatcatg attgaaagat tatttgtgat tctggataaa
    31261 aattttattt aacgattaat acagggatag tcgcacgttt tgctactttg tggctgacac
    31321 ttccaagtac aaatttcttt ttgtcttctg ctttacggtt acttaaaaca acaatttcgt
    31381 atttaccgct attagcatgt tttaatagtt cttcttttgc attaccacga acaattattt
    31441 ggtcataatc aataccataa tcttctaaag ctttgcgtgt tttttccaat cgcttgcttc
    31501 gttcttcagt aagtttgttt aaatgaacac ctgctttaat tgatgcttga gcatcttgtt
    31561 cgctgattgc gtttaaaact gttacgactg taccttcgcc agctaattta gacacttctt
    31621 ttagtgcttt ttcatttttt aactgagtgt ctacaccaag taatatattt ttatacatta
    31681 gtacatccct cctcacttac attctaataa ataataatgg atttttccat ttaaaattaa
    31741 agattttgtg atctttgtgt ttgttgtata ttttcaaaaa ataaaatagg tgaaacgatc
    31801 attatttcat ttcacttcaa tgctttttat ccaaaataaa gttataatgt agttaatact
    31861 gttcgaaaag aagaggtaaa gaatgacaaa acatgaacaa attttagatt atattgaatc
    31921 gttatctata ggctctaaaa tttcggtgcg taaaatcgct aaatttttaa acgtatctga
    31981 agggacagca tatcgtgcaa ttaaagatgc tgataaaatg ggcatggtcg caacgataga
    32041 tcgtgttggt acggttcgaa tcgaaaaacg taatcgtaat gaaattgaac atttaacttt
    32101 taatgaaatt gtcaatatta ttgatggcca agtgttaggt ggcaataaag gtatcactaa
    32161 gatggtatct aaatttgcaa ttggagcaat ggaactcaaa gatattttaa aatatattgg
    32221 tccaaagaca ttattgattg tcggaaatcg tgaagatgtg cagattgaag ctttaaaaag
    32281 agatacggct atattaatta ctggtggatt taagccatct aataaagtta ttaattttgc
    32341 gaatgaacat gatttacctg ttctatcatc aagttatgat acatttctag ttgcaaatat
    32401 tatcaacaaa gcacttttta atcagaaaat acgaaaagat atcttaatag ttcaagatat
    32461 aatgacgcca ttagatgatt tatctgtgct gtttgatacg atgaaaatag cagattataa
    32521 gcgtatggcg aatcgaactg gccatacaag atttccggta gtcaatgagt cttataaatt
    32581 agtaggtatt gtaacaagtc gagagatgat taatacaaag gacgacgacg aaatagataa
    32641 agtgatgacg cgaaatccta tttatgtaaa tgctatgagt acagtagcaa gttgtgcgca
    32701 tatgatgatt tgggaaggta ttgaattgat tcctgtcgtt tcgagtaata aaaagacagt
    32761 gggtgtgatt aatagacaag atgttttaaa gtctatgcaa cttttaggaa gacagcctca
    32821 gatgggtgaa acaattaacg atcaaatcgc caaatatata acaatgaatc aagatggaat
    32881 tacagtagaa gtttcaccac ttttaatcaa tcattacgga acagtaagta aagcggcatt
    32941 tgtaagtatt atagaagaaa caatacaata tgaaatgcgt aaatttaaaa aaggtaatgt
    33001 catgatagag aacttaaata tcgtgtatat taaaacagta cctattgaat cacatataac
    33061 agtgcgcttt ggcatactag acgtcggaag aaacttcgcc aaaatagaag tgaacatgca
    33121 tagtcaaaac gacaaagtag cgagcgctct cgtgatatgc caaatgtttg acgaagtata
    33181 ggaacttagc agagaaaata ggcataagat agatttcgca caaacgttgg ggcccaactt
    33241 gaacattata gtaagctgac attacgtcag cttctatgtt ggggcccacc ccaacttgca
    33301 tggtctgtag aatttctttt cgaaattctc tatgttgggg ccccacccca acttgcattg
    33361 tttgtagaaa ttgggggtcc aatttctcta tgttgtggcc cctgaattaa ataaagcttg
    33421 ttatttgtgc atttttattt ggtcaactgt tgctagtata atattgcttg atgtcctata
    33481 atcgttatga actaaatagt atgaaaatat atatatctaa ataattaaaa cgggcaaata
    33541 gtttaatatg gtagtaacaa tttttataat ttagatattt tgatatagac taatgcttaa
    33601 attaagcaac ttttctaaaa aagggagtat gaaaatgatt agtactttga atgaaattat
    33661 gaaatgtatc gaagacaacg atacaattat tatacacaga catgttagac cagatcctga
    33721 tgcatatggt tcacaacttg gtttaaaata ctatattcag cagaaatttc cgcaaaagca
    33781 agtatttgca gtgggtgaag cggaatcatc attaagtttt attggagagt tagataacat
    33841 tgatgataaa acatatcaag atgcgcttgt aattgtatgt gatactgcca atgctccacg
    33901 aattgacgat gaacgttata gtgcaggtcg taaacttatt aaaatagatc atcatcctgc
    33961 agttgatcag tatggtgata ttaatttagt taatacgaac gcgtcatcta caagtgaaat
    34021 catttacgat ttaatctcac attttaatga tgaagcaatt gttaataaag atatcgcgag
    34081 cgttttatat cttggtatcg tcggtgatac tgggcgattc ctttttaaca atacctcaga
    34141 acatactatg gaaattgctg gaaagttaat tgggcatgat attgatcata atgcgctttt
    34201 aaataaaatg atggagaagg acccaaaaat gttgccgttt caaggttatg ttttacaaca
    34261 tttcgaactt atggatgatg gattctgcca agttaaaata actgaagatg tattggagca
    34321 attcggtatt cagccaaatg aagcatctca gtttgttaat acaattgctg acatcaaagg
    34381 cttgaaaata tgggtatttg cagtcgatga aggtaatgaa atcagatgtc gattacgttc
    34441 taaagggcaa ttgattatta atgatattgc gcaagatttt ggtggcggtg gtcatccgaa
    34501 tgcgtcagga gtttcagtgg acagctggga tgaatttgag caacttgcta cagctttacg
    34561 cacaaaactt aactaataga aaggggccat tcaatcatgg tggcatattt aaatattcat
    34621 acggcttatg atttgttaaa ttcaagctta aaaatagaag atgccgtaag acttgctgtg
    34681 tctgaaaatg ttgatgcact tgccataact gacaccaatg tattgtatgg ttttcctaaa
    34741 ttttatgatg catgtatagc aaataacatt aaaccgattt ttggtatgac aatatatgtg
    34801 acaaatggat taaatacagt cgaaacagtt gttctagcta aaaataatga tggattaaaa
    34861 gatttgtatc aactatcatc ggaaataaaa atgaatgcat tagaacatgt gtcgtttgaa
    34921 ttattaaaac gattttctaa caatatgatt atcattttta aaaaagtcgg tgatcaacat
    34981 cgtgatattg tacaagtgtt tgaaacccat aatgacacat atatggacca ccttagtatt
    35041 tcgattcaag gtagaaaaca tgtttggatt caaaatgttt gttaccaaac acgtcaagat
    35101 gccgatacga tttctgcatt agcagctatt agagacaata caaaattaga cttaattcat
    35161 gatcaagaag attttggtgc acatttttta actgaaaagg aaattaatca attagatatt
    35221 aaccaagaat atttaacgca ggttgatgtt atagctcaaa agtgtgatgc agaattaaaa
    35281 tatcatcaat ctctacttcc tcaatatgag acacctaatg atgaatcagc taaaaaatat
    35341 ttgtggcgtg tcttagttac acaattgaaa aaattagaac ttaattatga cgtctattta
    35401 gagcgattga aatatgagta taaagttatt actaatatgg gttttgaaga ttatttctta
    35461 atagtaagtg atttaatcca ttatgcgaaa acgaatgatg tgatggtagg tcctggtcgt
    35521 ggttcttcag ctggctcact ggtcagttat ttattgggaa ttacaacgat tgatcctatt
    35581 aaattcaatc tattatttga acgtttttta aacccagaac gtgtaacaat gcctgatatt
    35641 gatattgact ttgaagatac acgccgagaa agggtcattc agtacgtcca agaaaaatat
    35701 ggcgagctac atgtatctgg aattgtgact ttcggtcatc tgcttgcaag agcagttgct
    35761 agagatgttg gaagaattat ggggtttgat gaagttacat taaatgaaat ttcaagttta
    35821 atcccacata aattaggaat tacacttgat gaagcatatc aaattgacga ttttaaaaag
    35881 tttgtacatc gaaaccatcg acatgaacgc tggttcagta tttgtaaaaa gttagaaggt
    35941 ttaccaagac atacatctac acatgcggca ggaattatta ttaatgacca tccattatat
    36001 gaatatgccc ctttaacgaa aggggataca ggattattaa cgcaatggac aatgactgaa
    36061 gccgaacgta ttgggttatt aaaaatagat tttctaggtt tgagaaactt atcgattatt
    36121 catcaaatct taacacaagt caaaaaagat ttaggtatta atattgatat cgaaaagatt
    36181 ccgttcgatg atcaaaaagt gtttgaattg ttgtcgcaag gagatacgac tggcatattc
    36241 caattagagt ctgacggtgt aagaagtgta ttaaaaaaat taaagccgga acactttgaa
    36301 gatattgtgg ctgtaacttc tttgtataga ccaggtccaa tggaagaaat tccaacttac
    36361 attacaagaa gacatgatcc aagcaaagtt caatatttac atccgcattt agaaccgata
    36421 ttaaaaaata cttacggtgt tattatttat caagagcaaa ttatgcaaat agcgagcaca
    36481 tttgcaaact tcagttatgg tgaagcggat attttaagaa gagcaatgag taaaaaaaat
    36541 agagctgttc ttgaaagtga gcgtcaacat tttatagaag gtgcaaagca aaatggttat
    36601 cacgaagaca ttagtaagca aatatttgat ttgattctga aatttgcaga ttatggtttt
    36661 cctagagcac atgctgtcag ctattctaaa attgcataca ttatgagctt tttaaaagtc
    36721 cattatccaa attattttta cgcaaatatt ttaagtaatg ttattggaag tgagaagaaa
    36781 actgctcaaa tgatagaaga agcaaaaaaa caaggtatca ctatattgcc accgaacatt
    36841 aacgaaagtc attggtttta taaaccttcc caagaaggca tttatttatc aattggtaca
    36901 attaaaggtg ttggttatca aagtgtgaaa gtgattgttg atgaacgtta tcagaacggc
    36961 aaatttaaag atttctttga ttttgctaga cgtataccga agagagtcaa aacgagaaag
    37021 ttacttgaag cactgatttt agtgggagcg tttgatgctt ttggtaaaac acgttcaacg
    37081 ttgttgcaag ctattgatca agtgttggat ggcgatttaa acattgaaca agatggtttt
    37141 ttatttgata ttttaacgcc aaaacagatg tatgaagata aagaagaatt gcctgatgca
    37201 cttattagtc agtacgaaaa agaatattta ggattttatg tttcgcaaca cccagtagat
    37261 aaaaagtttg ttgccaaaca atatttaacg atatttaaat tgagtaacgc gcagaataat
    37321 aaacctatat tagtacagtt tgataaagtt aaacaaattc gaactaaaaa tggtcaaaat
    37381 atggcattcg tcacattaaa tgatggcatt gaaactttag atggtgtgat tttccctaat
    37441 cagtttaaaa agtacgaaga gttgttatca cataatgact tgtttatagt tagcgggaaa
    37501 tttgaccata gaaagcaaca acgtcaacta attataaatg agattcagac attagccact
    37561 tttgaagaac aaaaattagc atttgccaaa caaattataa ttagaaataa atcacaaata
    37621 gatatgtttg aagagatgat taaagctacg aaagagaatg ctaatgatgt tgtgttatcc
    37681 ttttatgatg aaacgattaa acaaatgact actttaggct atattaatca aaaagatagt
    37741 atgtttaata attttataca atcctttaac cctag
//
LOCUS       DBNENW010000007        37477 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNENW010000007 DBNENW010000000
VERSION     DBNENW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.007"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            31..984
                     /locus_tag="AC1LW8_000285"
     CDS             31..984
                     /locus_tag="AC1LW8_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772155.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1147..1350)
                     /locus_tag="AC1LW8_000286"
     CDS             complement(1147..1350)
                     /locus_tag="AC1LW8_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIP4772156.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1649..2137
                     /locus_tag="AC1LW8_000287"
     CDS             1649..2137
                     /locus_tag="AC1LW8_000287"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP4772157.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2130..3065
                     /gene="fmt"
                     /locus_tag="AC1LW8_000288"
     CDS             2130..3065
                     /gene="fmt"
                     /locus_tag="AC1LW8_000288"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIP4772158.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERINWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3062..4369
                     /gene="rsmB"
                     /locus_tag="AC1LW8_000289"
     CDS             3062..4369
                     /gene="rsmB"
                     /locus_tag="AC1LW8_000289"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIP4772159.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKFTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEKTETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4372..5466
                     /gene="rlmN"
                     /locus_tag="AC1LW8_000290"
     CDS             4372..5466
                     /gene="rlmN"
                     /locus_tag="AC1LW8_000290"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIP4772160.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLYELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5473..6216
                     /locus_tag="AC1LW8_000291"
     CDS             5473..6216
                     /locus_tag="AC1LW8_000291"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HIP4772161.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNRTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6213..8207
                     /gene="pknB"
                     /locus_tag="AC1LW8_000292"
     CDS             6213..8207
                     /gene="pknB"
                     /locus_tag="AC1LW8_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HIP4772162.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8435..9310
                     /gene="rsgA"
                     /locus_tag="AC1LW8_000293"
     CDS             8435..9310
                     /gene="rsgA"
                     /locus_tag="AC1LW8_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIP4772163.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9311..9955
                     /gene="rpe"
                     /locus_tag="AC1LW8_000294"
     CDS             9311..9955
                     /gene="rpe"
                     /locus_tag="AC1LW8_000294"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIP4772164.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9962..10603
                     /locus_tag="AC1LW8_000295"
     CDS             9962..10603
                     /locus_tag="AC1LW8_000295"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIP4772165.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10984..11172)
                     /gene="rpmB"
                     /locus_tag="AC1LW8_000296"
     CDS             complement(10984..11172)
                     /gene="rpmB"
                     /locus_tag="AC1LW8_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIP4772166.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11615..11989
                     /locus_tag="AC1LW8_000297"
     CDS             11615..11989
                     /locus_tag="AC1LW8_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP4772167.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12004..13650
                     /gene="fakA"
                     /locus_tag="AC1LW8_000298"
     CDS             12004..13650
                     /gene="fakA"
                     /locus_tag="AC1LW8_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HIP4772168.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13840..15900
                     /gene="recG"
                     /locus_tag="AC1LW8_000299"
     CDS             13840..15900
                     /gene="recG"
                     /locus_tag="AC1LW8_000299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIP4772169.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNNSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYGLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16103..16675
                     /gene="fapR"
                     /locus_tag="AC1LW8_000300"
     CDS             16103..16675
                     /gene="fapR"
                     /locus_tag="AC1LW8_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HIP4772170.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16680..17666
                     /gene="plsX"
                     /locus_tag="AC1LW8_000301"
     CDS             16680..17666
                     /gene="plsX"
                     /locus_tag="AC1LW8_000301"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIP4772171.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17659..18585
                     /gene="fabD"
                     /locus_tag="AC1LW8_000302"
     CDS             17659..18585
                     /gene="fabD"
                     /locus_tag="AC1LW8_000302"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIP4772172.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18578..19312
                     /gene="fabG"
                     /locus_tag="AC1LW8_000303"
     CDS             18578..19312
                     /gene="fabG"
                     /locus_tag="AC1LW8_000303"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIP4772173.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19682..19915
                     /locus_tag="AC1LW8_000304"
     CDS             19682..19915
                     /locus_tag="AC1LW8_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIP4772174.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            20031..20762
                     /gene="rnc"
                     /locus_tag="AC1LW8_000305"
     CDS             20031..20762
                     /gene="rnc"
                     /locus_tag="AC1LW8_000305"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIP4772175.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20909..24475
                     /gene="smc"
                     /locus_tag="AC1LW8_000306"
     CDS             20909..24475
                     /gene="smc"
                     /locus_tag="AC1LW8_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIP4772176.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISGAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAL
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24475..25725
                     /gene="ftsY"
                     /locus_tag="AC1LW8_000307"
     CDS             24475..25725
                     /gene="ftsY"
                     /locus_tag="AC1LW8_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIP4772177.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSESSTQDA
                     NDLAENAEVKKKPHKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            25712..26044
                     /locus_tag="AC1LW8_000308"
     CDS             25712..26044
                     /locus_tag="AC1LW8_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HIP4772178.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            26070..27437
                     /gene="ffh"
                     /locus_tag="AC1LW8_000309"
     CDS             26070..27437
                     /gene="ffh"
                     /locus_tag="AC1LW8_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIP4772179.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27872..28147
                     /gene="rpsP"
                     /locus_tag="AC1LW8_000310"
     CDS             27872..28147
                     /gene="rpsP"
                     /locus_tag="AC1LW8_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIP4772180.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28335..28838
                     /gene="rimM"
                     /locus_tag="AC1LW8_000311"
     CDS             28335..28838
                     /gene="rimM"
                     /locus_tag="AC1LW8_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIP4772181.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28838..29575
                     /gene="trmD"
                     /locus_tag="AC1LW8_000312"
     CDS             28838..29575
                     /gene="trmD"
                     /locus_tag="AC1LW8_000312"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIP4772182.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29678..30028
                     /gene="rplS"
                     /locus_tag="AC1LW8_000313"
     CDS             29678..30028
                     /gene="rplS"
                     /locus_tag="AC1LW8_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIP4772183.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30497..33103)
                     /gene="yfhO"
                     /locus_tag="AC1LW8_000314"
     CDS             complement(30497..33103)
                     /gene="yfhO"
                     /locus_tag="AC1LW8_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HIP4772184.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGSLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWLLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNKTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYAPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            33504..34388
                     /gene="ylqF"
                     /locus_tag="AC1LW8_000315"
     CDS             33504..34388
                     /gene="ylqF"
                     /locus_tag="AC1LW8_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIP4772185.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQHDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34372..35139
                     /locus_tag="AC1LW8_000316"
     CDS             34372..35139
                     /locus_tag="AC1LW8_000316"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIP4772186.1"
                     /translation="MTLTIKEVKQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            35248..36414
                     /gene="sucC"
                     /locus_tag="AC1LW8_000317"
     CDS             35248..36414
                     /gene="sucC"
                     /locus_tag="AC1LW8_000317"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIP4772187.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSVVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36436..37344
                     /gene="sucD"
                     /locus_tag="AC1LW8_000318"
     CDS             36436..37344
                     /gene="sucD"
                     /locus_tag="AC1LW8_000318"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIP4772188.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYEALLTVNK"
BASE COUNT        13899 a         5082 c         7262 g        11234 t
ORIGIN      
        1 caaacaaaat aacttaagag gagaaagttt atgaaaaaga cactgggatg tttactttta
       61 attatgcttt tagtcgtagc aggttgttct tttggtggga atcataaatt atcatcaaag
      121 aaatcagaag aatcaaagca agaaactgta aaaaaagaat cggaagaaga gaaagatcca
      181 gatttagaga aatatgaaga aatagagaag aaaatgaaag gaattaaaga tgcgccatct
      241 cttgataagt tggatccatt aatgacagaa aagtcgttta cgaatagtaa agggattcaa
      301 ggatggaaag attacaaaga attaatgggt aaagtggaac ttgcagatta tagatttact
      361 aaagattcaa aaggatcttc aataaaagat gttgatgcat tctttaaagg taagaaaggt
      421 ataaaaagga aagtgattga aacacacgat gatgtaaaac aagttgatta ttggtatgta
      481 gatccagatg gaaagaaaat tggcaattca aacacacctg ttttttacgc agaaattatg
      541 acaaaatata aagatggaaa gttagtttat gcatcagtcg aaccaggatc ttacgtaata
      601 cataaagatg atgcaattaa atatgacgat tattctaagt taaaaaaatt aagtcagcta
      661 actaaacttg atcatccaaa accagttcca tatagcgtag ctcaaatcaa atctttcgga
      721 gtacctttaa caagcgtttc atttatgaca catggatcaa aggatactaa agatgaagtg
      781 ttgccggcat tggcctattt cactttttca ccaaaaaatt atgaagacaa gtctaatcca
      841 gatccaaaag ttttaaattt agtacatatg gatttcttaa atgcatctag tgattttggt
      901 aacgcacatt ttgttgtttt aagtaaatat attaaagagt atgaatcaaa ctatgaaaca
      961 gcgtcagatg attctttaaa atagtattta ctgtgtgaaa aataaatagt gtactacatt
     1021 aaataatcgc aataataatc ccgataaaca atcagcatta ctgcttatca tatagagttc
     1081 gtaataacta taactctatg attcgcaaat aataaatgat tgtcatcggg atttattttt
     1141 atcaatttat aaagtgacat taccttgttc atcagcaggt ttgaaaacag taatcactgc
     1201 actaataatt gctaaaatgt gtgggatacc tgtccaacag aatattaagt gtagaatacc
     1261 ttgcatattc ttgccggcat aaaatttatg aataccaaaa ctacctaaga acaatgctaa
     1321 taaaatataa ataactttgt ttacttgcat ttctttccct ccagttgaat tgcttataat
     1381 gacattagct tctcttttta ttatacccac ttttagttca aacattctag tttaagcatt
     1441 cccaatcatc taaatttcag ttattcaatc cttacaataa atttaggatt acacttcagt
     1501 tgcattgtat tattttacgt gtgaaatata cgtaatgaat cacatgacaa tctttaaatt
     1561 gaaaaatata cattctatga tgtaaggtcg catttttaat atatttacgt tataatggtt
     1621 tgatgaattt tattgaggag taagagctat ggcgattaaa aagttagtac cagcatcgca
     1681 tcctatttta acgaaaaaag cgcaagcagt tataaaattt gatgattcgt taaaaagatt
     1741 attacaagat ttagaagata caatgtatgc acaagaagca gctggcttat gtgcacctca
     1801 aattaatcag tcattgcaag tggcaatcat tgatatggaa atggaaggat tattacaact
     1861 tgttaatccg aaaattatta gtcaatcaaa tgaaacgata acagacttag aaggttcaat
     1921 tacattgcca gatgtttacg gcgaagtgac aagaagtaaa atgatagttg tcgaaagtta
     1981 tgacgtcaat gggaacaaag ttgaactaac tgcacatgaa gatgtagcaa gaatgatttt
     2041 gcatattata gatcaaatga acggtatccc ttttacagaa cgtgcggacc gtattttaac
     2101 agataaagaa gtggaggcat attttataaa tgactaaaat aatatttatg ggtacaccag
     2161 acttttcaac aactgtttta gaaatgctta ttgcagaaca tgatgtcatt gcagtcgtaa
     2221 cgcaaccaga tcgacctgtt ggacgtaaac gtgttatgac accaccacca gttaaaaaag
     2281 ttgcaatgaa atatgattta cctgtatatc agcctgaaaa attaagtgga tcagaagaat
     2341 tagaacaatt gcttcaatta gatgtagatt taattgtaac tgctgctttt ggacaattat
     2401 tacctgaatc attgttggca ttaccaaaac ttggggcaat taatgtacat gcatcattgt
     2461 taccgaagta tagaggtggt gcaccaattc atcaggcaat tatcgatggt gaacaagaaa
     2521 ccggcataac aattatgtat atggttaaaa aattagatgc gggtaatatt atttcgcaac
     2581 aagcaattaa aatagaagaa aatgataatg tcggtacgat gcatgataaa ttaagtgtat
     2641 taggggcaga tttattaaaa gaaactttac catctattat agagggcaca aatgaaagtg
     2701 tacctcaaga tgatacgcaa gcaacatttg cttccaatat tcgacgcgaa gatgagcgaa
     2761 ttaactggaa taaaccagga agacaagtgt ttaatcaaat tcgtggatta tcaccatggc
     2821 cagttgctta tacaactatg gatgacacta acttgaaaat atacgatgct gaactcgttg
     2881 agactaataa gataaacgag cctggaacca ttatagaaac gactaaaaaa gccattattg
     2941 ttgctacaaa tgataatgaa gctgttgcaa ttaaagatat gcaattagct gggaaaaaga
     3001 gaatgttagc tgccaattat ttaagtggtg cgcaaaacac actagtaggg aagaaactta
     3061 tatgatagaa aacgtgagaa gtcttgcttt tgacacgatt caagatatat taaatgaagg
     3121 tgcgtatagt aacttgcgta tcaatgaagt gttgtcagaa aatgaattaa atgcaatgga
     3181 taaggcttta tttacagaaa ttgtctacgg aaccgttaaa agaaaattta cgttagattt
     3241 ttatttaaag ccttttgtga aaacaaaaat taaggcatgg gttaggcaat tattatggat
     3301 gagtatttat caatatgttt atttagataa agttccaaat cacgccatca ttaatgaagc
     3361 agttgaaata gcaaaagaac gcggtggcta tcataatggt aatgtcgtaa atggtatttt
     3421 acgtacaatg atgcgtagtg acttacctga ttttaatgaa attgcagatc ctaaaaaaag
     3481 aatggcaatc gagtatagta tgccgaagtg gattatagat cattgggcaa cacattatgg
     3541 tctcgaaaaa actgaaacaa ttttacagtc atttttagaa acgacatcaa caactgtgcg
     3601 tgccaacctg acgcgagcat cattagatga tattattgaa aagttgcaag acgaaggtta
     3661 tgacgttgaa aaagatcatg acttacctta ttgtctccat ataggaggac aaccaattat
     3721 tcattctcgt tcatttaaag atggattcgt ttcaattcaa gataaaagct caatgtttgt
     3781 tgcacacatt atgaatgtag accgacatga tcacgtatta gatgcatgta gtgcacctgg
     3841 cggtaaagct tgtcacattg ctgaagtttt aatgccagaa gggcaagttg acgcttcaga
     3901 tatacatgat cacaaaatag acttaattaa ttttaatata aaaaaattac gattaacaaa
     3961 tattaaagct tttcaacatg atgcgacaaa accttatgat aaaacatacg ataagatact
     4021 tgttgatgca ccatgtagcg gattaggtgt aatgagacat aagccggaga ttaagtatac
     4081 tcaaagcaaa caacatattg agtcactagt tgaattacag cttgaaatat tggaaaatgt
     4141 aaaaaacaat gtaaaaatag gtggcgaaat catctattca acatgtacaa ttgagcaact
     4201 agaaaatgaa aacgtgattt atacgttttt gaaaaataat aaaaacttcg aatttgaacc
     4261 gtttcaacat ccgataactg gagagttggt caaaacgtta caaatcatgc cgcaagactt
     4321 taattcagat ggattcttta tcactaagat aaaaagaaag gacaattagg aatgataact
     4381 gctgaaaaga aaaagaagaa taaatttctt ccaaattttg acaagcaatc aatatattca
     4441 ttgcgatttg acgaaatgca aaactggctc gttgaacaag gtcaacaaaa atttcgagcg
     4501 aaacagattt ttgaatggtt atatcaaaaa agagtagatt cgattgatga aatgacgaac
     4561 ttatcgaaag acttacgaca gcttttaaaa gataacttta ctgttacaac tttaacaact
     4621 gtagtaaaac aagaaagtaa agacggtaca attaaattct tatatgaatt acaagatggc
     4681 tatacaattg aaactgtttt aatgagacat gattatggaa attcagtatg tgtaacgaca
     4741 caagtaggtt gtcgtatcgg atgtacgttt tgtgcttcta cacttggcgg cttaaaaaga
     4801 aaccttgaag ctggcgaaat tgtttcacaa gttttaacag ttcaaaaagc ccttgatgct
     4861 acagaagagc gcgtatctca aattgtcata atgggtatcg gtgaaccatt tgaaaattat
     4921 gatgaaatga tggacttttt aagaatcgtc aatgatgata atagtttaaa tattggtgca
     4981 cgtcacatta cagtatcaac atcaggtatc attcctagaa tatacgactt tgcggatgaa
     5041 gatatccaaa ttaattttgc tgtaagctta cacgccgcaa aagatgaagt gcgatcacgc
     5101 ttgatgccaa ttaaccgtgc atataatgtt gagaagttaa tcgaagcaat tcaatattat
     5161 caagaaaaaa caaatcgtcg tgttactttt gaatatggtc tgtttggtgg tgtgaatgac
     5221 caactagaac atgcaagaga attagcacat ttaataaaag gcttaaactg ccatgttaac
     5281 ttaattcctg tcaaccatgt tccagaaaga aattatgtga aaacggctaa aaatgatatc
     5341 tttaaatttg aaaaagaatt aaagagacta ggaattaatg ccacaatacg tcgtgaacaa
     5401 ggttcggata ttgacgcagc ttgtggtcaa ttaagagcaa aggaacgaca agtagaaacg
     5461 aggtaaagac aaatgctaga ggcacaattt tttactgata ctggacaaca tagagataag
     5521 aatgaagatg cgggtggtat tttttataat cgaactaatc aacaactttt agttctgtgt
     5581 gatggtatgg gcggccataa agcaggagaa gttgcaagta aatttgttac agatgagttg
     5641 aaatcccgtt ttgaagcgga aaatcttata gaagaacatc aagctgaaaa ttggttgcgt
     5701 aataatataa aagatataaa ttttcagtta tatcactatg cacaagaaaa tgcagaatat
     5761 aaaggtatgg gtacaacatg tgtttgtgca cttgtttttg aaaaatcagt tgtgatagca
     5821 aatgtcggtg attctagagc ctatgttatt aatagtagac aaattgaaca aattactagt
     5881 gatcactcat ttgttaatca tcttgtttta acgggtcaaa ttacgccgga agaagcattt
     5941 acacatccac aacgtaatat tattacgaag gtgatgggca cagataaacg tgtgagtcca
     6001 gatttgttta ttaagcgatt aaatttttat gattatttat tattaaattc agatggatta
     6061 actgattatg ttaaagacaa tgaaattaag cgtttgttag taaaagaagg tacaatagaa
     6121 gatcatggtg atcaattaat gcaattggca ttagataacc attcgaaaga taacgttact
     6181 ttcatactcg cggctattga aggtgataaa gtatgatagg taaaataata aatgaacgat
     6241 ataaaattgt agataagctt ggcggcggtg gcatgagtac cgtttatctt gctgaagata
     6301 cgatacttaa cattaaagtt gcaattaagg cgatttttat accacctaga gaaaaagaag
     6361 aaacattaaa acgttttgaa cgagaagtac ataactcatc acagctatca catcaaaata
     6421 tagtaagtat gatcgatgtt gatgaagaag atgactgtta ctacttagta atggaatata
     6481 ttgaaggtcc gactttgtct gagtatattg aaagtcatgg gccattaagt gttgacacgg
     6541 cgattaattt tacgaatcaa atattggatg gtattaaaca tgcgcatgat atgcgtattg
     6601 tacatagaga tattaagcca caaaatatat taattgacag caataaaacg ttgaaaatat
     6661 ttgattttgg aattgctaaa gctttaagtg agacgtcttt aactcagact aatcatgtgt
     6721 taggtactgt gcagtacttt tcgccagaac aagcaaaagg tgaggcaacg gatgaatgta
     6781 cagatattta ttctataggt attgtgttat atgaaatgct tgttggtgaa ccacccttta
     6841 atggagaaac tgcagttagc attgcgatta aacatattca ggattctgtg ccaaatgtga
     6901 caacagatgt acgtaaggat attccgcaat ctttaagtaa tgtcatttta cgcgctacag
     6961 aaaaagacaa agcgaatcgt tacaaaacaa ttcaagaaat gaaagatgat ttgagtagtg
     7021 ttttacatga aaatcgagcg aatgaagatg tctatgaact cgataaaatg aaaacgatag
     7081 cggtaccttt gaaaaaagaa gatctagcaa agcatattag tgaacataag tcgaatcaac
     7141 ctaaacgtga aacgacgcaa gtacctattg taaatgggcc tgctcatcat cagcaattcc
     7201 aaaagccaga aggtacggtt tacgaaccaa aacctaaaaa gaaatcaaca cgaaagattg
     7261 tgctcttatc actaatcttt tcgttgttaa tgattgcact tgtttctttt gtggcaatgg
     7321 caatgtttgg taataaatac gaagagacac ctgatgtaat cgggaaatct gtaaaagaag
     7381 cagagcaaat attcaataaa aacaacctga aattgggtaa aatttctaga agttatagtg
     7441 ataaatatcc tgaaaatgaa attattaaga caactcctaa tacaggtgaa cgtgttgaac
     7501 gtggtgacag tgttgatgtt gttatatcaa aaggccctga aaaggttaaa atgccaaatg
     7561 tcattggttt acctaaggag gaagccttgc agaaattaaa atcgttaggt cttaaagatg
     7621 ttacgattga aaaagtatat aataatcaag cgccaaaagg atacattgca aatcaaagtg
     7681 taaccgcaaa tactgaaatc gctattcatg attctaatat taaactatat gaatctttag
     7741 gcattaagca agtttatgta gaagactttg agcataaatc ctttagcaaa gctaaaaaag
     7801 ccttagaaga aaaagggttt aaagttgaaa gtaaggaaga gtatagtgac gatattgatg
     7861 agggtgatgt gatttctcaa tctcctaaag gaaaatcagt agatgagggg tcaacgattt
     7921 catttgttgt ttctaaaggt aaaaaaagtg actcatcaga tgtcaaaacg acaactgaat
     7981 cggtagatgt accatacact ggtaaaaatg ataagtcaca aaaagttaaa gtttatatta
     8041 aagataaaga taatgacggt tcaactgaaa aaggtagttt cgacattact agtgatcaac
     8101 gtatagacat tcctttaaga attgaaaaag gaaaaacagc aagttatatt gttaaagttg
     8161 acggtaaaac tgtagctgaa aaagaagtca gctatgatga tgtataaata taattgaagt
     8221 aaatgtaccg aggtttctat ttggaagtct cggtattttt atgttgagat tgcggtagtt
     8281 ttaaaatgct tcatgtcatc atatacaggt gagcattgtc tatcaattct gtatcactct
     8341 cacaatattt atatcatttg aaatgatcat ttatatatta attttccagt ctttgatata
     8401 ataaatgcag taaaattaat cgagaggtgc cattttgaag acaggtcgaa tagtgaaatc
     8461 aattagtggg gtatatcaag tagacgttaa tggcgaacgt ttcaatacaa aaccacgagg
     8521 attatttaga aagaaaaaat tttcaccggt agttggtgat atagtggaat ttgaagtaca
     8581 aaacattaac gaaggctata ttcatcaagt gtttgagcgg aaaaatgagt tgaaaagacc
     8641 acctgtaagt aatatagata cactagtaat tgtaatgagt gctgtcgagc caaatttttc
     8701 aacgcaatta ttagatcgat ttttagttat tgcacattcg tatcagttaa atgcgagagt
     8761 tttggtgact aaaaaagata aaacaccaat tgaaaagcag ttcgaaatta atgagttgtt
     8821 gaaaatatat gaaaatattg gctatgagac tgaatttatt ggaaatgatg atgatcgaaa
     8881 aaaaattgta gaagcttggc cagctggact tatagtactt agtggtcaat caggtgtcgg
     8941 taagtccact ttcttaaatc attatcgtcc agaacttaat cttgagacaa atgatatatc
     9001 aaaatcatta aatcgaggaa agcatactac aagacatgtc gaactattcg aacgtcaaaa
     9061 cggttatatt gcagacacac ctggattcag tgctttagat tttgatcata tagataaaga
     9121 tgaaataaaa gattattttc ttgaattaaa tcgatatggt gaaacatgta agtttaggaa
     9181 ttgtaatcat atcaaagaac ctaattgtaa tgttaagcat caattagaga tagggaatat
     9241 tgcgcaattt agatacgacc attatttaca actatttaat gaaatttcaa atagaaaggt
     9301 tagatattaa atgacaaaac tatatccatc attattatct gttgattttt tggatttaca
     9361 acatgaatta aaacgacttg aagaagcagg tgtcgacgga gttcattttg atgttatgga
     9421 tggtcaattt gtgcctaata tatctattgg tttaccaata ttagatgcag taagaaaagg
     9481 cacaacatta cctatagacg tacatttgat gattgaaaat ccagaaaagt atattgcatc
     9541 atttgcagaa catggtgccg atatgatttc aattcatgtc gaatcaacgc ctcatattca
     9601 tcgtgctatt caaatgatta aacatttaga taaaaaagct ggtgtagtaa ttaatcctgg
     9661 tacaccaata tcacaaattg aacctatttt agacattgtt gattatgtac tagtgatgac
     9721 agttaaccca gggtttggtg gtcaatcatt tattgatcaa tgcgtagaaa aaatagcggg
     9781 tcttaatgct attaaaatgg aacgtcaatt aaactttgat attgaagttg atggaggcgt
     9841 aaataccgat acagcgaaag tttgtgttga aaatggtgct acaatgctag taacaggttc
     9901 atttttcttt aaacaagagg attataaaaa agtcacacaa gcattgaaag gttgagtgta
     9961 tatgcatata aatttattat gttctgatcg acacttgccg caagatattt gggccaaaag
    10021 taatgaaggt aaatggggcg gcgttgatag aggtgctttg attttattga agcatcaaat
    10081 tatccctttt ttctcagtgg gagactttga ttcagtcagt aaagaagaac gccaacttct
    10141 aacagaacag ttacaaatca aaccagttca agctgaaaaa gctgatacgg atttagcttt
    10201 agcggttgat aaagctgttg cacttggatt tgatagtatt acaatttatg gtgcaacagg
    10261 cggacgatta gatcactttt ttggggcaat tcagttatta ttgaaaaaag catattataa
    10321 acatgatgtt catatagaag ttatcgatca acaaaataaa attgaattat tgcctaaagg
    10381 tcaacataca gttgaaaaag ataagagtta tccgtacatt tcatttatac cgatgactga
    10441 tgatgtagaa ctttctctag caggttttaa atataattta gctagacaaa tgcttaatat
    10501 aggttctact ttaactattt caaatgaaat tgagtctttg caagcgaaag taactgtaca
    10561 tgatgggttg attttgcaaa ttagaagtac agatttaaat taagtatatt caaatttgtg
    10621 aggattttta ataaagtgca tgagttattt aataaagtga cgtttataga tagaatttat
    10681 aacataataa tagatgaatc acgctgtaat taatgaagat tatggcgtga tttattattg
    10741 aaaggaaaat tgtaggtcct agtacattga tttatgtgcc aaaatcgata ttgaacccat
    10801 aagtccaatt tgacagagaa ccattattaa acgtattaac caaattatat tgatagttcg
    10861 aatttcacta tgaaccatta tgtattgttt ggtaacaaaa aaagaactaa gaggttgagc
    10921 tcttagttct taagtaaatg tgtcctccac ataaggtcac attttttatg tgcaatatat
    10981 ttattaaact ctagttactt taccagattt taaagcacgt gcagaaaccc aaactttttt
    11041 aggtttaccg tcaactagga ttctaacttt ttgaaggtta gcgttccatc tacgtttagt
    11101 agagtttaaa gcgtgtgaac gtctgttacc agtcgaagct ttacgacctg ttacgaaaca
    11161 ttgtttaccc atatgagtac ctcctttaat aaatataaat acacataact acttatatac
    11221 ttaattaaga tagcatagtt tcatttgaaa aacaatgaat aattttcaca taaaagtcaa
    11281 aaatactgag ttttgtgata taattgtaga ctgtgaagtt atgtagtatg atattttaga
    11341 gaaataagtg aatgaaacat ttaaaatttt tatttaatga tactacatct attaaaagaa
    11401 aacgcctata gataatcgtc atgagtgtaa attcagttta ctcaaaatat aataggtgat
    11461 ttaagcgtaa gtcattctga taagttgtac cattgatatt ttataaaaat aatcaatgaa
    11521 acttaaagcg tttatgacta cactaagtgg taaaatttta aaagtgatta gggaaaacga
    11581 ataataaact ttgaaattta ggagggacaa agatatgaca ttagagattt caaatgatta
    11641 cggtaaaatt gatatttcaa acgaagtgat tgcttcggtt gtaggtggaa aggccgttga
    11701 atgttatggt attgtaggta tggcatctag acaacaagtt agagatggta ttgcggaaat
    11761 actaggacat gaaaattatg ctaaaggcat caaagtaact gaaaataatg gcgtagtgga
    11821 tatagatatg tacattattg taagttacgg tgtgaaaata tctgaagttg ccaataatgt
    11881 acaatcaaca gtgaaatata ctttggaaaa atcacttaat gtatcagtaa attcaatcaa
    11941 tatatatgta caaggtgtac gtgtgaataa tacaggcaag aaagcttagg aggacaactt
    12001 gaaatgatta gcaaaattaa tggtaaatta tttgccgata tgattataca aggggcacaa
    12061 aatttatcta acaatgcaga tttggtagat tctttgaatg tgtatccagt gccagatggt
    12121 gatacaggaa caaatatgaa tcttactatg acttcaggtc gcgaagaagt agagaataat
    12181 ttgtcgaaaa atatcggcga attaggtaaa acattctcga aaggtttact aatgggtgca
    12241 agaggtaact ctggtgtcat cttgtcacaa ttattcagag gattttgtaa aaatattgaa
    12301 agtgaatctg aaattaattc aaaattgtta gctgaaagtt ttcaagctgg tgttgaaacg
    12361 gcatataaag ctgttatgaa accagttgaa ggtacaatac ttacagttgc aaaagatgct
    12421 gcgcaagctg caatagaaaa agcaaataat actgaagatt gtatagaatt aatggagtac
    12481 attattgtaa aagccaatga atcacttgaa aacacaccaa acttattagc tgtacttaaa
    12541 gaagttggtg ttgttgatag tggcggtaaa ggtttgttat gcgtttacga aggattctta
    12601 aaagcgctta aaggtgaaaa agttgaagcc aaagttgcaa agatagataa agatgaattt
    12661 gtacatgatg aacatgattt ccatggtgta attaatactg aagatattat ttatggctat
    12721 tgtactgaaa tgatggttcg ttttggaaag aataaaaaag cctttgatga acaagaattc
    12781 aggcaagata tgagtcaatt tggtgattct ttattagtca ttaatgatga agaaattgtg
    12841 aaagttcacg tgcataccga atacccaggt aaagtgttta attatggtca acaatatggt
    12901 gaattaatta aacttaaggt tgaaaatatg agagaacagc accgtgaagt gattcgaaaa
    12961 gaacagcaca cagctaaacc gaaaatggaa acggttgaaa cagcaattat tactatttct
    13021 atgggtgaag gtatttcaga gatattcaaa tcaatgggtg ccacacatat cattagtggt
    13081 ggacaaacga tgaatccttc tacagaagat atcgttaaag tcattgaaca atcaaaatgt
    13141 aaacgtgcaa ttattttacc gaataataaa aatattttaa tggcaagtga acaagcagcg
    13201 agtattgttg atgcagaagc agttgttatt ccaacgaaat ctattcctca aggtataagc
    13261 gcactattcc aatatgatgt ggacgcaaca cttgaagaaa ataaagcgca aatggctgat
    13321 tcagtaaata acgttaaatc tggttcatta acgtacgctg ttcgtgatac gaaaattgat
    13381 ggcgttgaga ttaaaaaaga cgcgtttatg ggcttgattg aagataagat tgtaagcagc
    13441 caaagtgatc aattaacaac ggttactgag ttgttaaatg agatgttagc agaagatagt
    13501 gaaatattga ctgtgattat tggtcaagat gcagagcaag cagttacaga taacatgata
    13561 aactggatcg aagagcaata tccagatgta gaagtggaag ttcatgaagg tggacaacca
    13621 atttatcaat atttcttttc agtagaataa aaatttaaaa taaaaaacta ccaatgataa
    13681 atcatcagtt ggtagttttt tattttgcta ttttagtgtt attgcgggtt aaaagtatca
    13741 ttctcgagtt gctaacaatg tcatgttcaa cttagtcatg ataaaataaa taacatacta
    13801 aatgatacgt aaaatcaaat aaaacatagg tgatttattt tggctaaagt aaacttaata
    13861 gaaagtccat attctctttt acaattaaaa ggtataggtc ctaagaaaat agaagtattg
    13921 caacaactaa atattcatac agtggaagat cttgttcttt atttgccaac tagatatgaa
    13981 gataatacag tgattgattt gaatcaagca gaagatcaat ctaacgttac gatagaagga
    14041 caagtatata cagctccagt agttgcattt tttggaagaa ataattcaaa attaaccgtt
    14101 catttaatgg taaataatat tgctgtcaaa tgtatttttt tcaatcaacc gtatttaaaa
    14161 aagaaaatcg aattaaatca aactataact gttaaaggta agtggaatag agttaaacag
    14221 gaaattactg gtaatagggt tttctttaat tcacaaggga cacaaactca agaaaacgca
    14281 gatgttcaat tagaaccagt ctatcgtatt aaggaaggta ttaaacaaaa gcaaatacga
    14341 gaccaaatta gacaagcgtt aaatgatgtg acaattcatg aatggttaac tgatgaacta
    14401 agagaaaaat ataaattaga gaccttggac tttactttga acacattaca tcatcctaaa
    14461 agtaaagagg atttattacg tgctcgtaga acctatgcat ttactgaact gtttttattc
    14521 gaattacgta tgcaatggct aaatagatta gaaaagtcat ctgacgaagc aattgaaatt
    14581 gattatggct tagaccaagt taaatcattt attgatcgtt taccttttga actaactgaa
    14641 gcacagaaat ccagtgttaa tgaaattttt agagatttaa aagcaccaat acgtatgcat
    14701 cgattacttc aaggtgatgt aggttcagga aaaacagtag ttgctgcaat ttgtatgtat
    14761 gcgttaaaaa cggctggtta tcaatcagca ttgatggtac caactgaaat tttagcagag
    14821 caacatgctg aaagtttaat ggctttattt ggagattcta tgaacgttgc attgttaact
    14881 gggtcagtaa aaggtaagaa acgaaagata cttttagaac aacttgaaaa tggtacgatt
    14941 gattgtttaa ttggaaccca tgctttgatt caagatgatg tgattttcca taatgttggt
    15001 ttagtaatta cagatgaaca acatcgattt ggtgtgaatc aacgccagct tttaagagaa
    15061 aaaggtgcaa tgacgaatgt gttatttatg acagcaaccc cgataccaag aacactagca
    15121 atatcagttt ttggtgagat ggatgtgtct tcaattaaac aattaccaaa aggtcgtaaa
    15181 cctatcatta ctacttgggc aaagcatgag caatacgata aagttttgat gcaaatgacc
    15241 tcagagttga aaaaaggtcg tcaagcatat gtcatttgcc cgctaataga aagttctgag
    15301 catctcgaag atgttcaaaa tgttgtcgca ttgtacgagt ctttacaaca gtattatggt
    15361 gtttcccgtg tagggttatt gcatggtaag ttatctgccg atgaaaaaga tgaggtcatg
    15421 caaaagttta gtaatcatga gatagatgtt ttagtttcta ctactgttgt tgaagtaggt
    15481 gttaatgtac cgaatgcaac ttttatgatg atttatgatg cggatcgctt tggattatca
    15541 actttacatc agttacgcgg tcgtgtaggt agaagtgacc agcaaagtta ctgtgtttta
    15601 attgcatccc ctaaaacaga aacaggaatt gaaagaatga caattatgac acaaacaacg
    15661 gatggatttg aattgagtga acgagactta gaaatgcgtg gtcctggaga tttctttggt
    15721 gttaaacaaa gtgggttgcc agatttctta gttgccaatt tagttgaaga ttatcgtatg
    15781 ttagaagttg ctcgtgatga agcagctgaa cttattcaat ctggcgtatt ctttgaaaat
    15841 acgtatcaac atttacgtca ttttgttgaa gaaaatttat tacatcgcag ttttgactaa
    15901 ttaccatgct gatttgtcaa tttgagtgca acacttcgtt aattgagtga tatgacactt
    15961 gaactattta aatgtaaagt ggtattttaa caatttataa atttccgact aaataatagc
    16021 taaataatac agttatttgt tgagtcggtt aaatagaaag tgttatgata tgtgaggaat
    16081 gtttaagact aggtactaaa aaatgagggg tgagacgttg aaactaaaga aagataaacg
    16141 tagagaagca atcagacaac aaattgatag caatcccttc atcacagacc atgaactaag
    16201 cgacttattt caagtgagta tacaaacaat tcgtttagat cgcacttatt taaacatacc
    16261 agaattaagg aagcgtatta aattagttgc tgaaaagaat tatgaccaaa taagttctat
    16321 tgaagaacaa gaatttattg gtgatttgat tcaagtcaat ccaaatgtta aagcgcaatc
    16381 aattttagat attacatcgg attctgtttt tcataaaact ggaattgcgc gtggtcatgt
    16441 gctgtttgct caggcaaatt cgttatgtgt tgcgctaatt aagcaaccaa cagttttaac
    16501 tcatgagagt agcattcaat ttattgaaaa agtaaaatta aatgatacgg taagagcaga
    16561 agcacgagtt gtaaatcaaa ctgcaaaaca ttattacgtc gaagtaaagt catatgttaa
    16621 acatacatta gttttcaaag gaaattttaa aatgttttat gataagcgag gataaaatta
    16681 tggttaaatt agcaattgat atgatgggtg gcgacaatgc gcctgatatc gtattagaag
    16741 ccgtacaaaa ggctgttgaa gactttaaag atctagaaat tatacttttc ggtgacgaaa
    16801 aaaagtataa tctgaaccat gaacgaatcg aatttagaca ttgttctgaa aagattgaaa
    16861 tggaagatga gcctgttaga gcgattaaac gtaaaaaaga tagctcaatg gtaaaaatgg
    16921 ctgaagctgt gaaatctggt gaagcagatg gatgtgtgtc agcaggtaat actggtgctt
    16981 taatgtcagc tggtttattc attgttggac gtattaaagg tgtagctaga ccggctttag
    17041 tagtaacatt gccaacgatt gatggaaaag gttttgtatt tttagacgtt ggtgcaaatg
    17101 ctgatgctaa acctgaacac ttattacagt atgcgcaact aggggatatt tatgctcaaa
    17161 aaatcagagg tattgataat ccgaaaatct cattattaaa tataggtacc gagccagcta
    17221 aaggtaatag tttaacgaaa aaatcatatg agttattaaa tcaagatcat tcattgaatt
    17281 ttgttgggaa tattgaagcg aagacattaa tggatggcga tacagatgtt gtagttaccg
    17341 atggctatac tgggaacatg gtccttaaaa atttagaagg tactgcaaaa tcaatcggta
    17401 aaatgttaaa agatacgatt atgagtagta ctaaaaataa attagcaggt gcaatattga
    17461 agaaagattt agcagaattc gctaaaaaga tggattactc agaatacggt ggttcagtat
    17521 tattaggatt ggaaggtact gtagttaaag cacacggtag ttcaaatgct aaagcttttt
    17581 attctgcaat tagacaagcg aaaatcgcag gagaacaaaa tattgtacaa acaatgaaag
    17641 agactgtagg tgaatcaaat gagtaaaaca gcaattattt ttccgggaca aggtgcccaa
    17701 aaagttggta tggcacaaga tttgtttaat aacaatgatc aagcaactga aattttaact
    17761 tcagcagcaa agacgttaga ctttgatatt ttagagacaa tgtttactga tgaagaaggt
    17821 aaattgggtg aaactgaaaa cacgcaacca gctttattga cgcatagttc ggcattatta
    17881 gcagcgctaa aaaatttgaa tcctgatttt actatggggc atagtttagg tgaatattca
    17941 agtttagttg cagctgacgt attatcattt gaagatgcag ttaaaattgt tagaaaacgt
    18001 ggtcaattaa tggcgcaagc atttcctact ggtgtaggaa gtatggctgc agtattgggc
    18061 ttagattttg ataaagtcga tgaaatttgt aagtcattat catctgatga caaaataatt
    18121 gaaccagcaa acattaattg cccaggtcaa attgttgttt caggtcacaa agctttaatt
    18181 gatgagctag tagaaaaagg taaatcatta ggtgcaaaac gtgtcatgcc tttagcagta
    18241 tctggtccat tccattcatc gctaatgaaa gtgattgaag aagatttttc aagttatatt
    18301 aatcaatttg aatggcgtga tgctaagttt cctgtagttc aaaatgtaaa tgcgcaaggt
    18361 gaaactgaca aagaagtaat taaatctaat atggtcaagc aattatattc accagtacaa
    18421 ttcattaact caacagaatg gctaatagac caaggtgttg atcattttat tgaaattggt
    18481 cctggaaaag ttttatctgg cttaattaaa aaaataaata gagatgttaa gttaacatca
    18541 attcaaactt tagaagatgt gaaaggatgg aatgaaaatg actaagagtg ctttagtaac
    18601 aggtgcatca agaggaattg gacgtagtat tgcgttacaa ttagcagaag aaggatataa
    18661 tgtagcagta aactatgcag gcagcaaaga gaaagctgaa gcagtagtcg aagaaatcaa
    18721 agctaaaggt gttgacagtt ttgcgattca agcaaatgtt gccgatgctg atgaagttaa
    18781 agcaatgatt aaagaagtag ttagccaatt tggttcttta gatgttttag taaataatgc
    18841 aggtattact cgcgataatt tattaatgcg tatgaaagaa caagagtggg atgatgttat
    18901 tgacacaaac ttaaaaggtg tatttaactg tatccaaaaa gcaacaccac aaatgttaag
    18961 acaacgtagt ggtgctatca tcaatttatc aagtgttgtt ggagcagtag gtaatccggg
    19021 acaagcaaac tatgttgcaa caaaagcagg tgttattggt ttaactaaat ctgcggcgcg
    19081 tgaattagca tctcgtggta tcactgtaaa tgcagttgca cctggtttta ttgtttctga
    19141 tatgacagat gctttaagtg atgagcttaa agaacaaatg ttgactcaaa ttccgttagc
    19201 acgttttggt caagacacag atattgctaa tacagtagcg ttcttagcat cagacaaagc
    19261 aaaatatatt acaggtcaaa caatccatgt aaatggtgga atgtacatgt aatatatttg
    19321 agctaaagct caagataaag acgtagtaat caataaattg ataactgata agcggaacgc
    19381 ctgagctaaa gctaattgac gcagtggttg actggtcatc caatggagaa ttgcctgacc
    19441 tagtcaactt tgcgggggaa attctaagca acctagataa ggttcgagaa tttctcccta
    19501 agaaacacta atcaataaat tgataagaag attagaggaa cgcttgagct aaagctcatg
    19561 cataagaaac actaatcaat aaattgataa gtgtttctaa aatttctact tgttttttag
    19621 aatttaaaat gggaaaatat aatagtctat gtataggcat ttttaaagga ggtgaatcga
    19681 cgtggaaaat ttcgataaag taaaagatat catcgttgac cgtttaggtg tagacgctga
    19741 taaagtaact gaagatgcat ctttcaaaga tgatttaggc gctgactcac ttgatatcgc
    19801 tgaattagta atggaattag aagacgagtt tggtactgaa attcctgatg aagaagctga
    19861 aaaaatcaac actgttggtg atgctgttaa atttattaac agtcttgaaa aataataaat
    19921 cttacatctg ggtcgtcagt attgtcgact cagttttttt ctttaattat caatagtttt
    19981 aacgtaaaat taaagatgat tcaagagcaa cacataaagg agataaaata atgtctaaac
    20041 aaaagaaaag tgagatagtt aatcgtttta gaaagcgctt tgatactaaa atgacagagt
    20101 taggctttac ttatcaaaat attgatttat accaacaagc attttcgcat tcgagtttta
    20161 ttaatgattt taatatgaat cgtttagacc ataatgagcg tttagagttt ttgggtgatg
    20221 cggtattaga attgacggtt tcacgatatt tatttgataa acatcccaac ttgccagaag
    20281 ggaatttaac aaaaatgcgt gccactattg tatgtgagcc ctcacttgta atatttgcga
    20341 ataaaattgg attgaacgaa atgattttac ttggtaaagg tgaagagaaa acagggggac
    20401 gtacaagacc atcattaata tcagatgcat tcgaagcatt tattggggca ttgtatttgg
    20461 atcaaggact agatatagtt tggaaatttg ctgagaaagt cattttccca catgtagaac
    20521 aaaatgagtt attaggcgtg gtagatttta aaacacaatt ccaagaatat gtgcaccagc
    20581 aaaataaagg tgatgtaacc tataatttaa taaaagaaga gggaccggca catcatcgtc
    20641 tattcacttc agaagttatt ctgcaagggg aagcaatagc tgaaggtaaa gggaaaacga
    20701 aaaaagaatc agaacaacgt gctgctgaaa gtgcctataa gcaattaaaa caaattaaat
    20761 agaaatttat atgatacgac atataggtgt tccatttagg atttaaggaa gaatttttaa
    20821 tattttatgg aacaacttcg aaatttaata tacaaatact aaaatatcgt atacatgtgt
    20881 tctttaaaat tgtgataagg agtttaggat ggtttattta aaatcaatag atgccattgg
    20941 atttaagtct tttgcagatc aaaccaatgt tcaattcgat aaaggtgtaa ctgcaattgt
    21001 tggtccaaat ggaagcggta aaagtaatat tacagatgct attaaatggg tgttgggtga
    21061 acaatcggct aaatcattac gtggctcaaa aatggaagat attatcttct caggtgcaga
    21121 acatcgcaaa gctcaaaatt atgctgaagt acagttaaga ttagataatc attctaaaaa
    21181 gctcagtgtt gatgaaaacg aagttattgt aacaagaaga ttgtatcgaa gtggtgaaag
    21241 tgagtactac ataaataatg accgtgcaag attaaaagat attgccgatt tatttttaga
    21301 ttctggattg ggaaaagaag cgtatagcat tatctcgcaa ggtagagttg atgaaatact
    21361 aaatgctaaa ccaattgata gacgtcaaat tattgaagaa tcggctggtg tacttaaata
    21421 taaaaaacgt aaagctgaat cattaaataa acttgaccaa acagaagata atttaacgag
    21481 agtagaagac attttatatg atttggaagg tcgtgtagaa cctctaaaag aggaggcagc
    21541 tatagctaaa gaatataaga cactttcaca tcaaatgaaa catagtgaca ttgtagttac
    21601 agtgcacgat attgatcaat atacaaatga caatagacaa ttagatcaac gtttaaatga
    21661 tttacaaggc caacaagcaa ataaagaagc tgacaaacaa cgtttaagcc aacaaattca
    21721 acaatataaa ggtaaacgtc atcaacttga taatgatgtt gaatcgctta attatcaatt
    21781 agtaaaagct acggaagcct ttgaaaaata tacgggacaa ttaaatgttt tagaagaacg
    21841 taagaaaaat caatctgaaa caaatgcacg atatgaagaa gaacaagaaa atttaatgga
    21901 gcttttagaa aatatatcaa atgagatttc tggagctcaa gatacttata agtctctgaa
    21961 aagtaaacaa aaagaactca atgctgtcat tcgtgaactt gaagaacaac tttatgtttc
    22021 agacgaagca catgatgaaa aattggaaga aattaaaaac gaatactata cattaatgtc
    22081 agagcaatca gatgttaaca atgatattcg ttttttaaag catactatag aagagaatga
    22141 ggctaaaaaa tcaagactag attctcgatt agttgaagtt tttgagcaat tgaaagatat
    22201 tcagggtcaa ataaaaacga caaaaaaaga atatcaacag accaacaaag aactttctgc
    22261 tgtagataaa gaaattaaaa atatagaaaa agatctcact gatacaaaaa aagcacaaaa
    22321 tgaatacgaa gagaaattgt atcaagcata tcgatatacc gaaaaaatga aaacacgtat
    22381 tgatagtttg gcaacgcaag aggaagaata tacttatttt ttcaatggcg tcaaacatat
    22441 tttgaaagct aaaaataaag aattaaaggg tattcatggt gcagttgcgg aaattattga
    22501 tgtgccatct aaattaactc aggcaattga aacagcatta ggtgcttcat tacaacatgt
    22561 cattgtagat tcagaaaaag atggacgcca ggctattcaa tttttaaaag aacgtaattt
    22621 aggtcgtgcg acgtttttac cattaaatgt tatacagagt agagtggtag cgactgatat
    22681 taaatctatt gctaaagagg caaacggatt tattagtatt gcttcggaag cagttaaagt
    22741 agcaccagaa tatcaaaata ttatcgggaa tttattaggt aatacgatta tcgttgatca
    22801 tttaaagcat gcaaatgaat tggcacgtgc gattaaatat cgaactcgta ttgttacttt
    22861 ggaaggtgat attgtaaacc ctggtggttc tatgactggt ggtggcgctc gtaagtccaa
    22921 aagtattctg tctcaaaaag acgagttgac aacaatgaga caccaattag aagattactt
    22981 gcgtcaaaca gaatcatttg aacaacaatt taaagagttg aagataaaaa gtgatcaatt
    23041 aagtgaactg tattttgaaa aaagtcaaaa gcataataca cttaaagagc aagtgcatca
    23101 ttttgaaatg gagctcgata gattaactac acaagaaaca caaataaaaa atgatcatga
    23161 agaattcgaa tttgaaaaaa atgatggtta tacgagtgac aaaagtcgac aaactttgag
    23221 tgaaaaagaa actcatttag aaagtattaa agcatcttta aaacgactag aagatgaaat
    23281 tgaacgctac acaaaacttt ctaaagaagg taaggaaagc gtaactaaaa cacaacaaac
    23341 gttacatcag aaacaatctg atcttgctgt ggttaaagag cgtattaaaa cacaacaaca
    23401 gacaatagat cgattaaata atcaaaatca acaaactaaa catcaattaa aagatgttaa
    23461 agaaaaaatt gcattgttta attcggatga agtgatgggc gaacaagctt ttcaaaatat
    23521 taaagatcaa attaatggtc aacaagaaac gagaacacgc ttatctgatg aattagataa
    23581 attgaaacaa caacgtattg agttgaatga acaaatcgat gcgcaagaag ctaaactaca
    23641 agtttgtcac caagatattt tagctatcga aaatcactac caagatatta aagctgaaca
    23701 atcaaagcta gatgtattaa ttcatcatgc gatagatcat ttaaatgatg aatatcaatt
    23761 gactgttgaa cgtgcgaaat ctgaatatac gagtgatgaa tcgattgacg cattacgtaa
    23821 aaaagttaag ttaatgaaga tgtcgattga tgaactaggt cctgtaaact taaatgcaat
    23881 tgaacaattt gaagagttaa atgaacgtta tacattttta agtgaacaac gtacagatct
    23941 tcgtaaagct aaagaaacat tagagcaaat tataagtgaa atggatcaag aggttactga
    24001 aagatttaaa gaaactttcc atgctattca aggacatttt acagctgtgt tcaaacaatt
    24061 gtttggtgga ggcgatgcag aattgcaatt aactgaagcc gattatttaa cagctggtat
    24121 tgatattgtg gtacaaccac cgggtaaaaa gttgcaacat ttatcgttac tgagtggtgg
    24181 tgagcgtgca ttaactgcta ttgctttact atttgcaatt ttaaaagtaa gatctgcacc
    24241 ttttgttata ttagatgagg ttgaagctgc actagatgaa gcaaatgtta ttagatacgc
    24301 aaaatattta aatgagttat cagacgaaac acaattcatt gttattacac accgtaaagg
    24361 aacaatggaa tttgcggata ggttatacgg tgtaacaatg caagaatcag gtgttactaa
    24421 acttgtgagt gtgaatttaa atacaataga tgatgtgttg aaggaggagc aataatgagc
    24481 ttttttaaac gcttaaaaga taagtttgca acaaataaag aaaatgaaga agttaaatcc
    24541 ttaacagaag aacaaggtca agacaaatta gaagatacac attctgaaag ttcaacgcag
    24601 gacgcaaatg atttagcaga aaatgctgaa gtgaaaaaga agccacacaa gttgagtgaa
    24661 gcggattttg atgacgatgg cttaatatca attgaagatt ttgaagaaat tgaagctcaa
    24721 aaaatgggtg ctaaatttaa agcaggactc gaaaaatctc gtcaaaattt ccaagaacaa
    24781 ttaaataatt tgatagcgag atatcgtaaa gtagatgaag acttttttga agctttagaa
    24841 gaaatgttaa tcactgcaga cgtcggtttt aatacagtga tgacgttaac tgaagaatta
    24901 cgtatggaag cacaacgacg taatattcaa gatactgaag atttgcgtga agtcattgtt
    24961 gaaaaaatcg tagagattta ccatcaagaa gatgataatt cagaagctat gaacttagaa
    25021 gatggtcgtt taaatgtcat tttaatggtt ggtgtgaatg gtgttggtaa aacaacaaca
    25081 attggaaaat tagcttaccg atataaaatg gaaggtaaaa aagtaatgtt agctgcgggc
    25141 gatactttta gagcgggtgc tattgatcaa ttgaaagttt ggggcgaacg tgttggtgta
    25201 gacgtaatta gccaaagtga aggttctgat ccagctgctg ttatgtatga tgcgattaat
    25261 gccgctaaaa acaaaggtgt tgatatttta atctgtgata ccgctggacg tttacaaaat
    25321 aaaacaaatc taatgcaaga attagaaaaa gttaagcgtg taattaatcg agcagtgcca
    25381 gatgcgcctc atgaagcatt actatgttta gatgctacaa ctggtcagaa tgcgttgtca
    25441 caagctagaa actttaaaga agtaacaaat gttacaggta ttgtattaac gaaattagat
    25501 ggtacagcca aaggtggtat cgtattagcc attcgtaatg aattgcacat tccagttaaa
    25561 tatgtaggtt taggtgagca attagatgac ttacaaccat ttaaccctga aagttatgtc
    25621 tacggcttat tcgctgatat gattgaacaa aatgaagaaa taacaacagt tgaaaatgat
    25681 caaattgtaa cagaagaaaa ggacgataat catgggtcaa aatgatttag ttaaaacgtt
    25741 acgaatgaat tatttgtttg atttttatca atccttattg acgaataaac aacgtaatta
    25801 tttggaatta ttttatcttg aagattattc tttaagtgaa atcgcagata cttttaatgt
    25861 gagtagacaa gcagtttatg ataatataag aagaactggc gatttagttg aagattatga
    25921 aaagaaattg gaattatacc agaaatttga gcaacgccga gaaatatatg atgaaatgaa
    25981 acaacattta agtaatccag aacaaataca acgttatatt caacaattag aagacttaga
    26041 atagatataa attgagggag gacatcgata tggcatttga agggttatca gaacgcttgc
    26101 aagcgacgat gcaaaaaatg cgtggtaagg gtaaacttac tgaagctgat ataaagataa
    26161 tgatgcgtga agtaagatta gcgttacttg aggctgacgt aaactttaaa gtggtaaaag
    26221 aatttattaa aacagtatca gaacgcgcat taggttccga tgtaatgcaa tcattaacac
    26281 cagggcaaca agttattaaa atagttcaag atgaattaac gcagttgatg ggtggagaaa
    26341 atacgtcgat taatatgtca aataaaccac ctactgttgt tatgatggtt ggtttacaag
    26401 gtgctggtaa aacaacaact gcaggtaaat tagcattatt gatgcgtaaa aaatacaaca
    26461 aaaaacctat gttagttgca gcagatattt atcgtccagc agcgataaat caattacaaa
    26521 cagtagggaa acaaattgat attcctgtat acagtgaagg agatcaagta aagccacaac
    26581 aaattgtaac taatgcatta aaacatgcta aagaagaaca tttagacttt gtaatcattg
    26641 atacagcagg tcgattacac atcgatgaag cattgatgaa cgaattaaaa gaagtaaaag
    26701 acattgctaa accaaacgaa attatgttag ttgtcgattc aatgacgggt caagatgctg
    26761 tcaatgttgc agaatctttt gacgatcaac ttgatgtcac aggtgttacc ttaactaaat
    26821 tagatggtga tacacgtggt ggtgcagctt tatctattcg ttcggtgaca caaaaaccaa
    26881 ttaaatttgt tggtatgagt gaaaagttag atggtttaga gctattccat cctgaacgta
    26941 tggcatcacg tattttaggt atgggtgatg tgttaagttt aattgaaaaa gcgcaacaag
    27001 atgtggatca agaaaaagca aaagatttag agaaaaagat gcgtgagtca tcgtttactt
    27061 tagatgattt tttagaacaa cttgatcagg tgaaaaatct aggaccactg gatgatatta
    27121 tgaaaatgat tccaggtatg aataaaatga aagggctaga taagcttaat atgagtgaaa
    27181 agcaaattga tcatattaaa gcgattatcc agtcaatgac gccggctgaa agaaacaatc
    27241 cagacacatt gaatgtatca cgtaaaaagc gtattgctaa agggtctggt cgttcattac
    27301 aagaagtcaa tcgtttgatg aaacaattta acgatatgaa gaaaatgatg aaacaattca
    27361 ctggtggcgg taaaggtaaa aaaggtaaac gcaatcaaat gcaaaatatg ttaaaaggta
    27421 tgaatttacc gttttaatgt ttaatataac acgctgattt ttgttgcaat gagatgtgca
    27481 tcccgaaagt tatactaaag aatataactt tatggggtgc atatttttag atacacaata
    27541 ataattaaaa actgaaattg cggatttgct agaacatgag gttattgttg ctaatattaa
    27601 taagttatat tgggggaacg tgtgcggtag aagtggtacc attcatgtcc ttacgggata
    27661 aattaactaa acacgtatgt ggtgaaggct actatgaatt tttataacct attaacagtc
    27721 taatttagta atcacatagt tattattgta agaaaaaggt tcaataattt tcctggtaaa
    27781 gaaaaaactc tttacaaaca ttcacacacc tgttaatatt atttcttgta gaaaataaaa
    27841 attaaaacat gacttaaagg agattttata aatggcagtt aaaattcgtt taacacgttt
    27901 aggttcaaaa agaaatccat tctatcgtat cgtagtagca gatgctcgtt ctccacgtga
    27961 cggacgtatc atcgaacaaa tcggtactta taacccaacg agcgctaatg ctccagaaat
    28021 taaagttgac gaagcgttag ctttaaaatg gttaaatgat ggtgcgaaac caactgatac
    28081 agttcacaat atcttatcaa aagaaggtat tatgaaaaaa tttgacgaac aaaagaaagc
    28141 taagtaattt agcgtaaaat tgttctaaca ataagaataa ctcgtttaca ctgacagtta
    28201 ttactcaatg atacgttggg aatatcacat gttagtaata tagaacgtat tgggtaccat
    28261 aatggtgccc tttttctttg aattattttc aattaaaata gaagtgttca aagcatagag
    28321 ttggaggtaa tagaatgaga gttgaagttg gtcaaattgt taacacacat ggtattaaag
    28381 gtgaaattaa agtaaaatcc aattcagact ttacagacgt tcgttttcaa cccggtcaag
    28441 tgctgacagt tgtgcataac aataacgacc ttgaatatac tgttaagtca catagagtgc
    28501 ataaagggct tcatatgctt acatttgaag gtattaataa tattaatgat attgagcatt
    28561 taaaagggag ttctatttat caagagcgtg atcatgaaga tatcgtactt gaggaaaatg
    28621 aattttatta ttcagatatt ataggatgta cagtttttga tgatcaagaa acaccaatag
    28681 gtcgtgtaat taatatattt gaaacaggtg cgaatgatgt gtgggtgatt aaaggatcta
    28741 aagaatattt gattccttat attgctgatg ttgtaaaaga agtggatgtt gaaaataaaa
    28801 aaattatcat cacgccaatg gaaggattgt tggattaatg aaaattgatt atttaacttt
    28861 atttcctgaa atgtttgatg gtgttttaaa tcattcaatt atgaaacgtg cccaagaaaa
    28921 caataaatta caaatcaata cggttaattt tagagattat gcaattaaca agcacaacca
    28981 agtagatgat tatccgtatg gtggcggaca aggtatggtg ttaaagcctg aacctgtttt
    29041 taatgcgatg gaagacttag atgtcacaga acaaacacgc gttattttaa tgtgtccaca
    29101 aggcgagcca ttttcacatc agaaagctgt tgaattaagc aaggccgacc acatcgtttt
    29161 catatgcgga cattatgaag gttacgatga acgtatccga acacatcttg tcacagatga
    29221 aatatcaatg ggtgactatg ttttaactgg tggagaattg ccagcgatga ccatgactga
    29281 tgctattgtt agactgattc caggtgtttt aggtaatgaa cagtcacatc aagacgattc
    29341 attttcagat gggttattag agtttccgca atatacacgt ccgcgtgaat ttaagggtct
    29401 aacagttcca gatgttttat tgtctggaaa tcatgccaat attgatgcat ggagacatga
    29461 gcaaaagttg atccgcacat ataataaaag acctgactta attgaaaaat atccattaac
    29521 taatgcagat aagcaaatat tagaaagata taaaatagga ttgaaaaaag gttagtattg
    29581 tgctaacata atctgagtgt ggaaacacaa aaatcactat gatccgctgc tatatatttg
    29641 tcgaggcaag aacataggtt gaagaggaga atttataatg acaaatcaca aattaatcga
    29701 agcagtaact aaatcacaat tacgtacaga cttacctagt ttccgtcctg gtgatacttt
    29761 acgtgtacac gtacgtatca ttgagggtac tcgtgagcgt atccaagtat tcgaaggcgt
    29821 tgtaattaaa cgtcgtggcg gtggcgtttc tgaaacgttt acagttcgta aaatttcatc
    29881 aggtgttggc gtggaacgta cattcccatt acacacacca aaaattgaaa aaatcgaagt
    29941 taaacgtcgt ggtaaagtac gtcgtgctaa attatattac ttacgtagtt tacgtggtaa
    30001 agctgctaga atccaagaaa ttcgttaatc agcatttaaa caaagctatg tatgagtcaa
    30061 attcgactca aacaataaag ccatctaggt cacttttatg gagtgatata gatggctttt
    30121 tttgctgttt agttaatgta aagttgataa tgtaattgtt tattccttct ctattcataa
    30181 cgtgtttgct caaaatggtt atataaacaa aaggctggga cataaatcaa tgttctatct
    30241 ctacgaagtt atattggcag tagttgactg aacgaaaatg cacttgtaac aagccttttt
    30301 caattctagt caggggcccc aacacagaag ctgacgaaaa gtcagcttac aataatgtgc
    30361 aagttggcgg ggccccaaca tagaagctgg cggaaagtca gcttacaata atgtgcaagt
    30421 tggggtggga cgacgaaata aattttgcga aaatatcatt tctgtcccac tcccattaaa
    30481 tatatgcgta gctacattat ttttgtcttg cccatctcgt gaaaataata ctacatataa
    30541 tgccaaatat agtaattgtt ataagtaaat aatagtatgg tggggcatag ctcaattgaa
    30601 tctttgttat atttttaggt gctttaatgc cggtcataac accatttact tgttcaactt
    30661 taagactttg atcacctgat gtcgctttca taccttgatt atatgctgtt ggcaaaacaa
    30721 tatacccaga tgaattttta tttttagtaa tagtataacc ttgctttgtc ttactaactt
    30781 tgacagcctc taatgaattt gaagcgtctt taagcgtggt ataatcttcg ccgtatatcc
    30841 cttttaaatt tacacgatac ttacctttag gcaatgataa tctaatccta tctgaagctt
    30901 taatgcgtat tgttacgggt gttacaaagc gtcgatattt ataagtgagt ttatttcttt
    30961 cttgtgtata ttcattcact ttaacatcat gagctttatc cggcgaaagt aattctaaat
    31021 ccatttcaaa atacaaatct ttaaattgat tagaaactga ttttggcaac tgtacagtta
    31081 gaccaccatt attttgttta acttgtaata aatgttttgt aggagattgc caggctgcac
    31141 tatttaattt aattgttgaa tctgatagta aatttttatt ggctttaaaa tgtgtattaa
    31201 catctttagt cttgttagaa acaatccctt gcaacattgc ttgttcttta tctaatggag
    31261 attttaattc tttattggaa aagaccttat ttgtaatatg tgcacttgga taatggatgg
    31321 tatttttaga atgaatccaa cgaactttat tgtctttgtg ttcagactta attttaaatc
    31381 catatggtaa gttgtcatca tgattcactc taattcgatc attaacattc caaagtgata
    31441 gtaaattttg acgattgcca agtaatctat aagtgctgtt tttatcgatt ggcatattaa
    31501 tttggagtgt cttgtcataa tattttaata tgtctccatt aaaaatacta gaatataatg
    31561 aaatgccatt ataatgatat ataaatggtg aatttaatgc atagtctgac atataatcaa
    31621 tgcgattaaa tggaccagtt gcattttgat ttatcttttt tataagctgg tttacatagt
    31681 tactatggta atcatgttgt ttcaacgttg ataatgattg ttgataaggt ttgattgcca
    31741 tatttttatt gttatctaaa atgacgattt gttgaatcat aacgattaat actaatattg
    31801 caacggttaa ttttttataa cgccataaac taaattttaa aataacggca agcaccatta
    31861 gcaggataat acctactata agtgcaagtg ggtgtgtcgg tgatagtaat acataaagta
    31921 atgcgatgat gcttactggt attgttctga ttaaataata tttcatattt aatgttgata
    31981 aatgttgaat aaacaatccg caaagagcgc ttgatgatag tgctaagata tacacccaac
    32041 gcctttctgg aaatgaaaaa ccattaaaag cactgtcgaa atactgtgat aatgaaccaa
    32101 taaataataa ccatgttact attgcgaaaa gtctataaaa gtaaaaacga tacagtttga
    32161 atgacaataa tgcaacgata gtaagaattg aaatcgtaat ataaaatcca tcgctaaaga
    32221 aaaaataatg gtaatcaagt ggtgtcaaaa acggtatatc aacattggga ttttgctttc
    32281 tgtcattttc caaaaacgca gaaatgccag tgaataaacc aaatacactt gataacacac
    32341 tcaaaactgt agcagatatg acgcaaatta atttttgtgt tctagagaca atgtcatatt
    32401 tgtaagtgaa aatgagtcga tataaatagt agcaacctat aataatagct tgataataac
    32461 tgaaataaaa attgctaaat agtgttaagg ctatcgcaac aatgaaaata ccgattttgc
    32521 gttgttgaaa atatctttcc aaaccaagaa tcgataatgg taataaatat aataaacttc
    32581 cataaaatga ccaagtaaaa ttaaagtata taacgacagt tgacatgccg tataaaatcg
    32641 tagcgatcat atttgttgag cgtttaaagt gtaatatttt aaataagtag aaggtcacga
    32701 caaatgttat gatagctctt atcatggcca taataagttg gtttgtcggc caaaaatgta
    32761 ttgttgtcgg attaaatata ccaaccgttt ctcctatttt aatgaataga aaatttagcc
    32821 acattaaagg tgacagcgaa taataatatg atagtccttt catataatcg ccacctaatc
    32881 caaacgatgc atcatataaa ctagaaaaac tacttaaatg ttcatacaaa tacatttgaa
    32941 atggcatcat ttgacggaat ccatctccag ccccactaaa aacagtacca ttcacaatat
    33001 aatcatagat atgagtagaa aataaaataa gcgttaatat tacactaatg aaagttataa
    33061 caaagaattg tttgacgttt gaatttagcc acttttttaa cacaacatta tcctcaactt
    33121 tcaaatttaa aattaagttt aactgaaact aaagttaatg aggttcttga taggtaaaga
    33181 cgaagatgac tgtggaacag ataccttatc atagttactt aaactttgga tcattttcag
    33241 tttatcatta aacaaatata ttgaataata aaaatgtcat actgataaag atgaatgtca
    33301 cttaataagt aacttagatt taacaaatga tgatttttaa ttgtagaaaa cttgaaataa
    33361 tcacttatac ctaaatctaa agcattgtta agaagtgtga caatgttaaa ataaatatag
    33421 ttgaattaat gaatttgttc tataattaac aggttataga ttttaataat gagaaaagaa
    33481 ttgacgaaag taaggtgaat tgaatggtta ttcaatggta tccaggacat atggcgaaag
    33541 ccaaaagaga agtaagtgaa caattaaaaa aagtagatgt agtgtttgaa ctagtagatg
    33601 caagaattcc atatagttca agaaacccta tgatagatga agttattaac caaaaaccac
    33661 gtgttgttat attaaataaa aaagatatgt ctaatttaaa tgagatgtca aaatgggaac
    33721 aattttttat tgataaagga tactatcctg tatcggtgga tgctaagcac ggtaaaaatt
    33781 taaagaaagt ggaagctgca gcaattaaag cgactgctga aaaatttgaa cgtgaaaaag
    33841 caaagggact taagccaaga gcgattagag cgatgattgt tggaattcca aatgttggta
    33901 aatccacatt aataaataaa ctggcaaagc gtagtattgc gcagactggt aataaaccag
    33961 gtgtgaccaa acaacaacaa tggattaaag ttggtaatgc attacaacta ttagacacac
    34021 cagggatact ttggcctaaa tttgaagatg aagaagtcgg taagaagttg agtttaacag
    34081 gcgcgattaa agatagcatt gtgcacttag atgaagtagc tatatatgga ttgaactttt
    34141 taattcaaca tgatttagcg cgattaaagt cacattataa tattgaagtt cctgaagatg
    34201 cagaaatcat agcgtggttt gatgcgatag ggaaaaaacg tggcttaatt cgacgtggta
    34261 atgaaattga ttacgaagca gtcattgaac tgattattta tgatattcga aatgctaaaa
    34321 taggaaatta ttgttttgat atttttaaag atatgactga ggaattagca aatgacgcta
    34381 acaattaaag aagttaagca gttgattaat gcggttaata caatagaaga attagaaaat
    34441 catgaatgct ttttagatga gcgaaaaggt gttcaaaatg ccatagctag gcgcagaaaa
    34501 gcgttagaaa aagaacaagc tttaaaagaa aagtatgttg aaatgactta ctttgaaaat
    34561 gaaatattaa aagagcatcc taatgcaatt atttgtggta ttgatgaagt tggaagagga
    34621 cctttagcag gtccagtcgt tgcatgcgca acaattttaa attcaaatca caattatttg
    34681 ggccttgatg actcgaaaaa agtacctgtt acgaaacgtc tagaattaaa tgaagcacta
    34741 aaaaatgaag ttactgcttt tgcatatggt atcgcgacag ctgaagaaat agatgaattt
    34801 aatatttata aagctactca aatcgccatg cagcgagcta ttgatggatt atcagtacaa
    34861 ccaacgcatt tattgataga cgcgatgacg cttgataatg cactgcctca agtatcttta
    34921 atcaagggtg atgcaagaag tgtatctatt gcagcggcaa gtatcatggc aaaggttttt
    34981 cgtgatgatt atatgacaca gttatctaaa gattatcctg aatatggttt tgaaaaaaac
    35041 gcgggttacg gtaccaaaca acatttacta gcaatcgatg atattggcat tatgaaagag
    35101 catagaaaaa gctttgaacc tataaaatcg ttactgtaaa tcgtaaaatt caattaaatg
    35161 ataaaaaagt agcatttaag tttacaatag cgcttacatt ttttataatt gtaagggaac
    35221 ttaacctaag taacaggagg atggaagatg aatatccacg agtatcaagg taaagaaata
    35281 tttcgttcaa tgggcgttgc agttccagaa ggacgagtag catttactgc tgaagaagcg
    35341 gtggagaaag caaaagaatt aaattctgtt gtttatgttg taaaagcaca aattcatgct
    35401 ggaggtagag gtaaagcagg cggagtaaaa attgctaaat ctttatctga ggtagaaaca
    35461 tatgcaaaag aattattagg gaaaactttg gtgacacatc aaactggtcc agaaggtaaa
    35521 gaaattaagc gtttatatat cgaagaaggt tgtgctattc aaaaagaata ttacgttgga
    35581 ttcgttattg atcgtgcgac tgaccaagta acattgatgg cgtctgaaga agggggcact
    35641 gagattgaag aagttgctgc gaagactcct gaaaagatct tcaaagaaac tatcgatcca
    35701 gtaatcggac tttcaccatt ccaagcaaga cgaattgcgt ttaatattaa tattcctaaa
    35761 gaatctgtta acaaagcagc taaattctta ttagcacttt ataatgtatt cattgaaaaa
    35821 gattgttcaa tcgtagaaat caacccatta gttacaacag ctgatggtga tgtattggca
    35881 ttagatgcta aaattaattt tgatgataat gcattattca gacataaaga tgttgtagaa
    35941 ttacgtgatt tagaagaaga agatccgaaa gagattgaag cgtctaaaca tgatttatca
    36001 tacattgcat tagatggtga catcggatgt atggttaatg gtgcaggttt agccatggca
    36061 acaatggata cgattaatca tttcggtgga aacccagcca atttcttaga tgcaggcgga
    36121 agcgctacta gagaaaaagt aactgaagca tttaaaatca ttttaggtga tgaaaatgtt
    36181 aaaggtattt ttgtaaacat tttcggtggc attatgaaat gtgatgttat cgcagaaggt
    36241 atcgttgaag ctgtaaaaga agtagattta actttaccac tagttgtacg cttagaaggt
    36301 acaaatgttg agttaggtaa aaaaatctta aaagactcag gattagcaat tgaaccagca
    36361 gcaacaatgg ctgaaggtgc acaaaaaatt gttaaactag tcaaagaagc ataaggaaag
    36421 gatgggagca ctaagatgag tgtatttata gataagaata ctaaagtaat ggtacaaggt
    36481 attacagggt ctactgccct tttccataca aaacaaatgc ttgattatgg tacgaaaata
    36541 gtagcaggtg tgacgcctgg taaaggtggt caagttgttg aaggcgttcc tgttttcaac
    36601 actgttgaag aagctaaaaa tgaaaccggg gcaacggttt cagtcattta cgttccagca
    36661 ccatttgctg cagactcaat tttagaagca gctgatgcag acttagatat ggttatttgt
    36721 atcactgaac atattcctgt attagacatg gttaaagtta aacgctactt acaaggtaga
    36781 aaaacacgtt tagttggtcc aaactgtcca ggtgtgatta cagcagatga atgtaaaatt
    36841 ggtattatgc ctggctatat tcacaaaaaa ggtcatgttg gtgtagtatc tcgttcaggt
    36901 acattaacat atgaagcagt gcaccaattg actgaagaag gtattggtca aactacagct
    36961 gttggtattg gtggagaccc agtcaacgga acaaacttta ttgatgtttt aaaagcattc
    37021 aatgaagatg acgaaacgaa agcagttgtt atgattggtg aaatcggtgg tacggctgaa
    37081 gaagaagcag ctgaatggat taaagcgaat atgacaaaac cagttgtagg ctttatcggt
    37141 ggacaaacag cacctcctgg aaaacgtatg ggacatgctg gtgcaatcat ttcaggtggt
    37201 aaaggtactg ctgaagagaa aattaaaaca ttaaatagtt gtggtgtgaa aacagcggca
    37261 acaccttcag aaattggttc aacattaatt gaagctgcta aagaagcagg tatttatgaa
    37321 gcattattaa ctgttaataa ataaagttaa aagatgatat aaatggctat agcccattcc
    37381 atactttata aaagtattgg aaatgggcta tttgtcgtgc gatgaatttt agtacacttt
    37441 tcatattttg gaaagacaag ttaattattt tacctcc
//
LOCUS       DBNENW010000008        37340 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNENW010000008 DBNENW010000000
VERSION     DBNENW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.008"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            169..1287
                     /gene="ald"
                     /locus_tag="AC1LW8_000319"
     CDS             169..1287
                     /gene="ald"
                     /locus_tag="AC1LW8_000319"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIP4772189.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLQDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1382..2422
                     /gene="tdcB"
                     /locus_tag="AC1LW8_000320"
     CDS             1382..2422
                     /gene="tdcB"
                     /locus_tag="AC1LW8_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HIP4772190.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2453..3775
                     /locus_tag="AC1LW8_000321"
     CDS             2453..3775
                     /locus_tag="AC1LW8_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIP4772191.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3932..5323
                     /gene="norB"
                     /locus_tag="AC1LW8_000322"
     CDS             3932..5323
                     /gene="norB"
                     /locus_tag="AC1LW8_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HIP4772192.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVSSLLFITLLAITIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLAPKQNDTQL"
     gene            5721..37340
                     /gene="ebh"
                     /locus_tag="AC1LW8_000323"
                     /pseudo
     CDS             5721..37340
                     /gene="ebh"
                     /locus_tag="AC1LW8_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
BASE COUNT        14881 a         6481 c         6783 g         9195 t
ORIGIN      
        1 tatagtggta taattgcaca taatatttaa gaataagaat aatatattaa atatttatta
       61 acaaattata gataaaatat gaataattaa ttaatggtat ttacatattc ataaccaaag
      121 gataaaataa ttgtgaaatt attcacaaat gaaagaggag tgttgcaaat gttagttgca
      181 gtagtcaaag aattaaaaca aggtgaagga cgtgtagctt gcacacccga aaatgtgcgt
      241 aagttaacgg atgcaggaca taaagtaatt gttgaaaaaa atgctggcat tggttcagga
      301 ttttctaacg atatgtatga aaaagaaggc gctaagatcg taactcatga acaagcatgg
      361 gaagctgatc ttgttatcaa agtaaaagaa cctcatgaaa gcgaatatca atatttcaaa
      421 aagaatcaaa ttatctgggg atttttacat ttagcatctt caaaagaaat agtagaaaaa
      481 atgcaagaag ttggtgtaac tgcgattagt ggtgaaacca ttataaaaaa tggaaaagca
      541 gaattattag cgccaatgag tgctatagca ggtcaacgct cagcaattat gggagcttac
      601 tactctgaag cacaacatgg tggtcaaggt actttagtga ctggtgtaca tgaaaatgtg
      661 gatatacctg gtagtacata tgtgattttc ggtggtggag tagcagcaac aaatgcagca
      721 aatgttgcct tgggactaaa tgctaaagta atcattatcg agttaaacga tgatcgcatt
      781 aaatatttac aagatatgta tgcagaaaaa gatgtcacag tagtcaaatc aacaccagaa
      841 aatttagcag aacaaataaa aaaagcagat gtatttattt ctacaatttt aattcctggt
      901 gcgaaaccgc caaaattggt tactcgtgag atggttaaat caatgaaaaa aggttcagta
      961 ttaatcgata tagctattga ccaaggtgga actattgaaa caattagacc aactacaatt
     1021 tctgatccag tgtatgaaga agaaggtgtg attcattatg gtgtaccaaa ccaaccagga
     1081 gcagtcccaa gaacttcaac aatggcatta gcacaaggaa atattgatta tatattagaa
     1141 atttgtgaca aaggcttaga acaagcaatt aaagataatg aagccttaag tactggtgta
     1201 aacatttacc aaggacaagt gacaaatcaa ggattagctt catcacatga cctagattat
     1261 aaagaaatat taaatgttat cgaatagata ataatttaaa tgaaattgag tgaaatgaat
     1321 attttaaata tagcattata gtttggacta aaaatttaca aaacggaagg atgtaattat
     1381 tatgacaacc aacacagtta cattacaaac agcacatatt gtaagtttag gagatatcga
     1441 agaagctaaa gcaagcatta aaccatttat tcgtcgaaca cctctaatta aatcaatgta
     1501 tttaagccaa agtataacta aagggaatgt atttctaaaa ttagaaaata tgcaattcac
     1561 aggatctttt aaatttagag gcgctagcaa taaaattaat cacttaacag atgaacaaaa
     1621 agaaaaaggc attatcgcag catctgctgg gaaccatgca caaggtgttg ctttaacagc
     1681 taaattatta ggcattgatg caactatcgt aatgcctgaa acagcaccac aagcgaaaca
     1741 acaagcaaca aaaggctatg gggcaaaggt tattttaaaa ggtaaaaact ttaacgaaac
     1801 tagactttat atggaagaat tagcgaaaga aaatggcatg acaatcgttc atccatatga
     1861 cgataagttt gtaatggcag gccaaggaac aattggttta gaaattttag atgatatttg
     1921 gaatgtgaat acagtcatcg taccagttgg cggtggagga ttaattgcag gtattgccac
     1981 cgcattaaaa tcatttaacc cttcaattca tattatcggt gttcaatctg agaatgttca
     2041 tggtatggct gagtctttct ataagagaga tttaactgaa catcgagtgg atagcacgat
     2101 agcagatggt tgtgatgtaa aagttcctgg tgaacaaaca tatgaagtag ttaaacattt
     2161 agtagatgaa tttattcttg ttactgaaga agaaattgaa catgctatga aagatttaat
     2221 gcagcgtgcc aaaattatta ctgaaggtgc aggcgcatta ccaacagctg caattttaag
     2281 tggaaaaata aacaataaat ggcttgaaga taaaaatgtt gttgctttag tttcaggcgg
     2341 gaatgttgac ttaactagag tttcaggtgt cattgaacat ggactgaata ttgcagatac
     2401 aagcaagggt gtggtaggtt aaaacattta atcttaaaaa tgaggtgtaa ttatgtcaaa
     2461 tggtaaagaa ttacaaaaaa atataggttt cttctcagcg tttgctattg ttatggggac
     2521 agttattggt tcaggagtat tctttaaaat atcaaacgta acagaagtaa caggaacagc
     2581 aggaatggcc ttgtttgtat ggttcctagg cggcatcatt accatttgtg cggggttaac
     2641 agcagcagaa cttgctgctg caatccctga aacaggtggc ttaacgaagt atatagaata
     2701 tacatacggt gatttctggg gcttcctatc aggttgggcg caatcattta tttattttcc
     2761 agctaacgta gcagcattgt ctatcgtatt tgcgacacag ctaattaatt tattccattt
     2821 atctataggt tcgttaatac caatagcaat cgcatctgcg ttatctattg tgttgataaa
     2881 tttcctaggt tcaaaagcag gcggaatttt acaatcagtt actttagtaa ttaaactgat
     2941 tccaatcatc gttattgtaa tttttggtat ttttcaatct ggagatatca ctttttcatt
     3001 aattccaact acaggtaatt caggaaatgg tttctttaca gcaattggta gtggtttatt
     3061 agcaactatg tttgcatatg atggttggat tcatgtagga aatgttgcgg gggaacttaa
     3121 aaatcctaaa cgcgatttac ctttagcgat ttcagttggt atcggttgta ttatggctgt
     3181 gtatttatta attaacgcta cattcttatt aacgttgcca atagaactac ttgctggtaa
     3241 tttaaatgcg gcttcagata catcaaaaat attatttggt gaaaatggcg gtaagattat
     3301 tacaatcggt atattaattt ctgtttatgg tacgatcaat ggctatacta tgactggtat
     3361 gcgcgtacca tatgcaatgg ctgaaagaaa attattgcca tttagccact tattcgcaaa
     3421 attaacaaaa tctggcgcac catggtttgg cgcaattata caacttataa tcgctatcat
     3481 catgatgtca atgggagcat ttgatacaat tacaaacatg ctaatctttg ttatttggtt
     3541 gttctattgt atgtcatttg ttgcggtaat aattttaagg aaacgcgaac caaatatgga
     3601 gcgaccatat aaagtaccgt tatatccaat cataccttta attgcaattt tggcagggtc
     3661 atttgtatta attaatacac tgtttacaca atttatacta gcaatcattg gaattctaat
     3721 aacagcactt ggtataccag tttattatta taagaagaaa caaaaagcag cataaggtaa
     3781 gataactagc attaagaata aatggatgga ctactaataa atttaaagtt ttacacatta
     3841 aaatcaaaaa ccattcaatt attctatgga acagacaaat ttctgttatg gaatttgtct
     3901 gtttttcaaa agtataggga ggcaaataga gatggaaaag ccgtcaagag aggcatttga
     3961 aggcaataat aagttgttaa taggaattgt tttaagtgta ataacgtttt ggctatttgc
     4021 acaatcattg gttaatgttg taccaatact tgaagatagt ttcaatacag atattggaac
     4081 ggttaatatc gccgttagta taactgcttt attttcagga atgtttgtag taggagcagg
     4141 tggtcttgct gataaatatg gcagaattaa actcacgaac attggtatta tcttaaatat
     4201 attgggttca ttattaatca ttatttcaaa tattccttta ttacttatta taggaagatt
     4261 aattcaagga ctttcagcag catgtattat gcctgcaact ttgtctatta ttaagtcata
     4321 ttacattggg aaagatagac aacgcgcttt aagttattgg tcaattggct catggggcgg
     4381 ctctggtgtt tgttcatttt ttggaggtgc agttgcaacg cttttaggtt ggcgttggat
     4441 tttcatccta tcaattataa tttcattaat tgcgcttttt cttattaaag gcacacctga
     4501 aactaaatct aaatcgattt ctctaaataa atttgacatt aaaggtctgg ttcttttagt
     4561 cattatgctt ctcagtttaa atattttaat tactaaagga tcagaattag gtgttagctc
     4621 acttcttttt atcactttat tagcaattac aattggatct tttagtttat ttatagttct
     4681 tgaaaagcgt gctacaaatc ctttaatcga ttttaaatta tttaaaaata aagcttacac
     4741 aggtgcaaca gcttcaaact ttttgttaaa tggtgttgca ggaacattaa tagtagccaa
     4801 cacatttgtt caaagaggtt taggatattc ttcattgcaa gcaggaagtt tatcaatcac
     4861 ttatttagta atggtactaa ttatgattcg tgttggtgaa aagttacttc aaacactcgg
     4921 atgcaagaaa ccaatgttaa ttggaacagg agttcttatt gtcggagaat gtctcatttc
     4981 attaactttc ttgccagaaa tattgtatgt catttgttgt attataggtt atttattctt
     5041 tggtttagga ctagggatat atgctacacc atcaacagat acagcaattg caaatgcacc
     5101 gttagaaaaa gtaggcgttg ctgcaggtat ctataaaatg gcttctgcat taggtggagc
     5161 atttggcgtc gcattgagtg gtgcagtata tgcaatcgta tcaaatatga caaacattta
     5221 tacaggtgca atgattgcat tatggttaaa tgcaggtatg ggaatattat cattcgttat
     5281 cattttgtta cttgcgccta aacaaaacga cactcaatta tgataattga gaattaaatt
     5341 gaaatcatac aagtcgctac aatattaaac aaaaatataa accgattctt atgtgtcatt
     5401 attttaaatg aacataggga ttggtttttt attactcttt tacgctactt tatttataat
     5461 tattataaat tgtcacaaat tcaatttacc atacaatata ttttgtgtta ttatattctg
     5521 gatcataaat aaattgttca acacatagtt gtaatgtgtt tcaatacttt ttggatagat
     5581 tgcgaaattg tattgaatcg tcatcgtttt aaatttttaa atgagaatgg aatgagcatt
     5641 acaatataca agcaatcaaa agtaaataca ttcacaacac aacagagaca taacaacaag
     5701 ataaggagtg aacaatagct gtgaattatc gtgataaaat tcaaaagttt agtattcgta
     5761 aatatacagt tggtacattt tcaactgtca ttgcgacatt ggtattttta ggattcaata
     5821 catcacaagc acatgctgct gaaacaaatc aaccagcaag cgtggttaaa cagaaacaac
     5881 aaagtaataa tgaacagact gagaatcgag aatctcaagt acaaaattct caaaattcac
     5941 aaaatagtca atcattatct gctactcatg aaaatgagca accaaatatt agtcaagcta
     6001 atttagtaga tcaaaaagta gcgcaatcat ctactactaa tgatgaacaa ccagcatctc
     6061 aaaatgtaaa tacaaagaaa gattcggcaa cggctgcgac aacacaacca gataaagaag
     6121 aaggtaagca taaacaaaac gaaagtcaat ctgctaataa aaatggaaac gacaatagag
     6181 cggctcattt agaaaatcat gaagcaaatg tagtaacagc ttcagattca tctgataatg
     6241 gtaacgtaca acatgaccga aatgaattac aagcattttt tgatgcaaat tatcatgatt
     6301 atcgctttat tgaccgtgaa aatgcagatt ctggcacatt taactatgta aaaggcattt
     6361 ttgaaaagat taatacgtta ttaggcagta atgatccaat aaacaataaa gacttgcaac
     6421 ttgcatacaa ataattggaa caagctgttg ctttaattcg tacaatgcct caacgtcaac
     6481 aaactagccg tcgatcaaac agaattcaaa cgcgttctgt tgagtctaga gctgcagagc
     6541 ctagatcagt atcagactat caaaatgcaa attcatcata ttatgttgaa aatgctaatg
     6601 atggttcagg atatcctgta ggtacatata tcaatgcttc tagtaaaggg gcgccatata
     6661 atttaccaac tacaccatgg aatacattga aggcctctga ctcaaaggaa attgctctta
     6721 tgacagcgaa acaaactgga gatggctacc aatgggttat taagtttaat aaaggacatg
     6781 ctccacatca aaatatgatt ttctggtttg cattaccagc agaccaagtg ccagtaggaa
     6841 gaactgactt tgtaacagtt aattcagatg gaacaaatgt acaatggagt catggagcag
     6901 gagcaggtgc aaataaacca cttcaacaaa tgtgggaata tggagtaaat gatcctgatc
     6961 gttcacatga ctttaaaata agaaatagaa gtggccaagt aatatatagc tggccaactg
     7021 tccatgttta ttctttagaa gatttatcta gagcgagtga ttattttagt gaagctggag
     7081 caacagctgc tactaaagca tttggtagac aaaattttga atatattaat ggtcaaaaac
     7141 ctgctgaatc accgggtgtt cctaaagttt atactttcat cggtcaaggt gatgcaagtt
     7201 atacaatttc atttaaaaca caaggtccaa ctgttaataa attgtactat gcagcaggtg
     7261 ggcgtgcttt agagtacaat caattattta tgtacagtca actatacgtc gaatcaacgc
     7321 aagaccatca acaacgtctt aatggtttaa gacaagtggt taatcgtaca tatcgcatag
     7381 gtacaactaa acgtgtagaa gtgagtcaag gaaatgtaca aacgaaaaag gtattagaaa
     7441 gtacaaacct aaatatagat gattttgttg atgatccatt aagttatgtt aagacgccga
     7501 gtaataaagt gttaggtttt tacccaacta atgcaactac taacgctttt agaccggggg
     7561 gcgttcagga attaaatgaa tatcaattaa gtcaattatt tactgatcaa aaattacaag
     7621 aagcagcaag aactagaaac ccaataagat taatgattgg tttcgactat cctgatggtt
     7681 atggtaatag tgaaacttta gttcctgtta acttaacggt attacctgaa atccaacata
     7741 atattaaatt ctttaaaaat gacgatactc aaaacattgc tgaaaaacca ttttcaaaac
     7801 aagctgggca tccagttttc tatgtatatg caggtaacca agggaatgct tccgtgaatt
     7861 taggtggtag cgtaacatct attcaaccat tacgtattaa tttaacaagt aatgagaatt
     7921 ttacagataa agattggcaa attacaggta ttccgcgtac attacacatt gaaaactcga
     7981 caaatagaac taataatgct agagaacgta acattgaact tgttggtaat ttattaccag
     8041 gggattactt tggaacgata cgttttggac gtaaagaaca attattcgaa attcgtgtta
     8101 aaccacatac accaacaatt acaacgacag ctgagcaatt aagaggtaca gcattacaaa
     8161 aagtgcctgt taatatttcg ggaataccgt tggatccatc ggcattggtt tatttagttg
     8221 caccaacaaa tcaaactacg aatggtggta gtgaggcaga tcaaatacca tctggttata
     8281 cgatacttgc gactggtaca cctgatgggg tgcataatac aattactata cgaccgcaag
     8341 attatgttgt attcatacca cctgtaggta aacaaattag agcagtagtt tattataata
     8401 aagtagttgc atctaatatg agtaatgctg ttactatttt gccagatgac attccaccaa
     8461 caatcaataa tcctgttgga ataaatgcca aatactatcg aggcgacgaa gtcaacttta
     8521 caatgggagt ctctgataga cattctggta taaaaaatac aactattact actttgccga
     8581 atggttggac atcaaattta actaaatccg acaacaaaaa cggctcatta gctattacag
     8641 gtagagtgag tatgaatcag gcgtttaaca gtgatattac atttaaagta tcagcgacag
     8701 ataatgtcaa taatacgaca aatgatagtc aatctaaaca tgtgtcaatt catgtaggta
     8761 aaattagtga agatgctcat ccgattgtat taggaaatac tgagaaagtt gtagtagtca
     8821 atccgactgc tgtatctaat gatgaaaagc aaagcataat tactgccttt atgaataaaa
     8881 accaaaatat aagaggatat ttagcatcaa ctgatccagt aactgtcgat aataatggta
     8941 atgtcacatt acattaccgt gatggctcat caacaacgct tgatgctaca aatgtgatga
     9001 catacgaacc agttgtgaaa tctgaatatc aaactgccaa tgcagctaaa acagcaacgg
     9061 taacgattgc taaaggacaa tcatttaata ttggtgatat taaacaatat tttactttaa
     9121 gtaatggaca agctattcca agtggcacat ttacaaatat tacatctgat agaactattc
     9181 taactgcaca agaagttagt caaatgaatg caggcacgca gttatatcat atagttgctt
     9241 caaatgcata tcataaagac actgaagatt tctatattag tttaaaaatc gttgatgtga
     9301 aacaacctga aggcgatcaa cgtgtctatc gtacgtcaac atatgattta acaactgatg
     9361 aaatctcaaa agtaaaacaa gcttttatta atgcaaatag agatgtaatt acgcttgccg
     9421 agggtaatat ttcagttaca aatacaccta atggtgctaa tgtaagtact attacagtaa
     9481 atattaataa aggtcgatta acgaaatcat tcgcgtcaaa cctagctaat atgaatttct
     9541 tgcgttgggt taatttcccg caagattata cagtgacatg gacgaatgca aaaattgcaa
     9601 acagaccaac agatggtggt ttatcatggt ctgatgacca taaatcttta atttatcgtt
     9661 atgatgctac attaggcaca caaattacaa ctaatgatat tttaacaatg ctaaaagcga
     9721 ctactacagt gcctggattg cgtaataata ttactggtaa tgaaaaagca caagcagaag
     9781 ctggcggaag acctaacttt agaacgactg gttattcaca atcaaatgcg acaactgatg
     9841 gtcaacgtca atttacgttg aatggtcaag tgattcaagt gttagacatc atcaaccctt
     9901 caaacggtta tggtgggcaa cctgttacaa attcaaatac tcgtgcaaac catagtaact
     9961 caactgttgt taacgtaaac gaaccggcag ctaatggtgc tagcgcattt acaattgacc
    10021 acgttgtaaa aagtaattct acacataatg caagtgatgc agtttataaa gcacagttat
    10081 acttaacgcc atatggtcca aaacaatatg ttgaacattt aaatcaaaat acaggaaata
    10141 ctactgacgc tattaacatt tattttgtac caagtgattt agtgaatcca acaatttcag
    10201 taggtaatta cactaatcat caagtgttct caggtgaaac atttacaaat actattacag
    10261 cgaatgataa ctttggtgtg caatctgtaa ctgtaccaaa tacatcacaa attataggca
    10321 ctgttgataa taaccatcaa catgtttctg caacggcacc aaatgtgaca tcagcaacta
    10381 ataagacaat caatttatta gcaactgata caagcggcaa tacagctaca acttcgttca
    10441 atgtaacagt gaaacctttg cgtgataaat atcgagttgg tacttcatca acggctgcta
    10501 atcctgtgag aattgccaat atttcgaata atgcgacagt atcacaagct gatcaaacag
    10561 caattattaa ttcattaacg tttactgaaa cagtaccaaa tagaagttat gcaagagcaa
    10621 gtgcgaatga aatcactagt aaaacagtta gtaatgtcag tcgtactgga aataatgcca
    10681 atgttacagt aactgctact tatcaagatg gaacaacatc aacagtgact gtacctgtaa
    10741 agcatgtcat tccagaaatc gttgcacatt cgcattacac tgtacaaggc caagacttcc
    10801 cagcaggtaa tggttctagt gcatcagatt actttaagtt atctaatggt agtgccattc
    10861 cagatgcaac tattacatgg gtaagtggac aagcgccaaa taaagataat acacgtattg
    10921 gtgaagatat aacagtaact gcacatatct taattgatgg tgaaacaacg ccgattacga
    10981 aaacagcaac atataaagta gtaagtacag taccgaagca tgtctttgag acgaatagag
    11041 gtgctgtgtt cccaggtgta tctgatgtgt atgatgcgaa acaatatgtt aaaccagtta
    11101 atgattcttg gacacaaaat gcgcaacgta tgaatttcca atttacaaat tcatatggac
    11161 caagtaaaga tgtggttggt atttcaacaa gggatattcg agttacttat gataatcacc
    11221 aaacgcaaat tatcaagatt ttagcaaaag ttaaaccaga tccaccgcga attgatggca
    11281 actctgtgac ttataaagca ggtcttacta accaacaaat taaaattaat aatgtgttaa
    11341 gtagttcatc tattaaatta tttaaagcag ataatacgcc attaacgatt acaaacacga
    11401 cttatggtag tggtaatact gcagttgtaa cggtaagtga tgcgttacca aatggtgtga
    11461 ttaaggcaag gtcttctatc acaatgaata atgtgacgta cactactcaa gatgaacatg
    11521 gtcgagcgat agatgtaaca agaaatgaat ctgttgattc aaatgacagt gcaacagtaa
    11581 cagtgacacc acaattacaa gcaactactg aaggtgctgt atttattaaa ggtggcgacg
    11641 gttttgattt cggacacgta gaaagattta ttcaaaaccc gccacatggg gcaacggttg
    11701 catggcatga tagtccagat acatggaaga atacagtcgg taacactcat aaaactgcgg
    11761 ttgtaacatt acctaatggt caaggtacgc gtaatgttga agttccagtc aaagtttatc
    11821 cagttgctaa tgcaaaggcg ccatcacgtg atgtgaaagg tcaaaatttg actaatggaa
    11881 cggatgcgat gaactacatt acatttgatc caaatacaaa cacaaatggt atcactgcag
    11941 catgggcaaa tagacaacaa ccaaataacc aacaagcagg cgtgcaacat ttaaatgtcg
    12001 atgtcacata tccaggtatt tcagctgcta aacgagttcc tgttactgtt aatgtatatc
    12061 aatttgaatt ccctcaaact acttatacga caacggttgg aggcacttta gcaagtggta
    12121 cgcaagcatc aggatatgca catatgcaaa atgctactgg tttaccaaca gatggattta
    12181 cgtataaatg gaatcgtgat actacaggta caaatgacgc aaactggtca gctatgaata
    12241 aaccgaatgt ggctaaagtc gttaacgcaa aatatgacgt catctataac ggacatactt
    12301 ttgcaacatc tttaccagcg aaatttgtag taaaagatgt gcaaccagcg aaaccaactg
    12361 tgactgaaac agcggcagga gcgattacaa ttgcacctgg agcaaaccaa acagtgaata
    12421 cacatgcagg taacgtaacg acatacgctg ataaattagt tattaaacgt aatggtaacg
    12481 ttgtgacgac atttacacgt cgcaataata caagtccatg ggtgaaagaa gcatctgcag
    12541 caactgtagc aggtattgct ggaactaata atggtattac tgttgcagca ggtactttca
    12601 accctgctga tacaattcaa gttgttgcaa cgcaaggaag cggagagaca gtaagtgatg
    12661 agcaacgtag tgatgatttc acagttgtcg caccacaacc gaaccaagcg actactaaga
    12721 tttggcaaaa tgggcatatt gatatcacgc ctaataatcc atcaggacat ttaattaatc
    12781 caactcaagc aatggatatt gcttacactg aaaaagtggg taatggtgca gaacatagta
    12841 agacaattaa tgttgttcgt ggtcaaaata atcaatggac aattgcgaat aagcctgact
    12901 atgtaacgtt agatgcacaa actggtaaag tgacgttcaa tgccaatact ataaaaccaa
    12961 attcatcaat cacaattact ccgaaagcag gtacaggtca ctcagtaagt agtaatccaa
    13021 gtacattaac tgcaccggca gctcatactg tcaacacaac tgaaattgtg aaagattatg
    13081 gttcaaatgt aacagcagct gaaattaaca atgcagttca agttgctaat aaacgtactg
    13141 caacgattaa aaatggcaca gcaatgccta ctaatttagc tggtggtagc acaacgacga
    13201 ttcctgtgac agtaacttac aatgatggta gtactgaaga agtacaagag tccattttca
    13261 caaaagcgga taaacgtgag ttaatcacag ctaaaaatca tttagatgat ccagtaagca
    13321 ctgaaggtaa aaagccaggt acaattacgc agtacaataa tgcaattcat aatgcgcaac
    13381 aacaaatcaa tactgcaaaa acagaagcgc aacaagtgat taataatgat cgtgcaacac
    13441 cacaacaagt ttctgacgca ttaactaaag ttcgtgcagc acaaactaag attgatcaag
    13501 ctaaagcatt acttcaaaat aaagaagata atagccaatt agtaacatct aaaaataact
    13561 tacaaagttc tgtgaaccaa gtaccatcaa ctgctggtat gacgcaacaa agtattgata
    13621 actataatgc gaagaagcgt gaagcagaaa ctgaaataac tgcagcacaa cgtgttatag
    13681 acaatggaga tgcaacaccg cagcaaattt cagaggaaaa acatcgtgtc gataacgcat
    13741 taacagcatt aaaccaagcg aaacaaaatt taactgcaga tacacatacc ttagagcaag
    13801 cagtgcaaca attgaatcgc acaggtacaa cgactggtaa gaaaccagca agtattactg
    13861 cgtataataa ttcaatgcat gcgcttcaag cagaacttac aagtgctaaa aatagtgcta
    13921 atgctatcat tcagaagcca ataagatcag tgcaagaggt acaaacagca ttaacaaatg
    13981 taaatcgtgt taatgagcga ttaacgcaag caattaatca attagtacct ttagctgata
    14041 atagtgcttt aagaactgct aagacgaaac ttgatgaaga aatcaataaa tcagtaacta
    14101 ctgatggtat gacacaatca tcaatccaag catatgaaaa tgctaaacgt gcgggtcaaa
    14161 cagaatcaac aaatgcacaa aatgttatta acaatggtga cgcaacagac caacaaattg
    14221 ccgaagaaaa aacaaaagta gaagaaaaat ataatagctt aaaacaagca attgctggat
    14281 taacaccaga cttggcacca ttacaaactg caaaaactca gttgcaaaat gatattgatc
    14341 agccaacgag tacgactggt atgacaagca catctattgc agcatttaat gaaaaacttt
    14401 cagcagctag aactaaaatt caagaaattg atcgcgtact ggcatctcat ccagatgttg
    14461 caacgattcg tcaaaacgtg acagcagcga atgctgctaa aacagcactt gatcaagcac
    14521 gcaatggctt aacagttgat aaagcgcctt tagaaaatgc gaaaaatcaa ctacaacata
    14581 gtattgacac gcaaacaagt acaactggta tgacacaaga ctctataaat gcatacaatg
    14641 cgaagttaac agctgcacgt aataagattc aacaaatcaa tcaagtatta gcaggttcac
    14701 cgactgtaga acaaattaat acaaatacgt ctgcagcaaa tcaagctaaa tctgatttag
    14761 atcatgcacg tcaagcgtta acaccagata aagcaccgct tcaaactgcg aaaacgcaat
    14821 tagaacaaag cattaatcaa ccaacggata caacaggtat gacgaccgct tcgttaaatg
    14881 cgtacaacca aaaattacaa gcagcgcgtc aaaagttaac tgaaattaat caagtgttga
    14941 atggcaaccc aactgtccaa aatatcaatg ataaagtgac agaggcaaac caagctaagg
    15001 atcaattaaa tacagcacgt caaggtttaa cattagatag acagccagcg ttaacaacat
    15061 tacatggtgc atctaactta aaccaagcac aacaaaataa tttcacgcaa caaattaatg
    15121 ctgctcaaaa tcatgctgct cttgaaacaa ttaagtctaa cattacggct ttaaatactg
    15181 cgatgacgaa attaaaagac agtgttgcgg ataataatac aattaaatca ggtcaaaatt
    15241 acactgacgc aacaccagct aataaacaag cgtatgataa tgcagttaat gcggctaaag
    15301 gtgtcattgg agaaacgact aatccaacga tggatgttaa cacagtgaac caaaaagcag
    15361 catctgttaa atcgacgaaa gatgctttag atggtcaaca aaacttacaa agtgcgaaaa
    15421 cagaagcaac aaatgcgatt acgcatgcaa gtgatttaaa ccaagcacaa aagaatgcat
    15481 taacacaaca agtgaatagt gcacaaaatg tacatgcagt aaatgatatt aaacaaacga
    15541 ctcaaagctt aaatactgct atgacaggtt taaaacgtgg cgttgctaat cataatcaag
    15601 tcgtacaaag tgataattat gtcaacgcag atactaataa gaaaaatgat tacaacaatg
    15661 catacaacca tgcgaatgac attattaatg gtaatgcaca acatccagtt ataacaccaa
    15721 gtgatgttaa caatgcttta tcaaatgtca ctagtaaaga acatgcattg aatggtgaag
    15781 ctaagttaaa tgctgcgaaa caagaagcga acactgcatt aggtcaatta aataacttga
    15841 ataatgcgca acgtcaaaac ttacaatcgc aaattaatag tgcacatcaa atcgagacag
    15901 ttaatacgat taagcaaaat gcaacaaact taaatagtgc aatgggtaac ttaagacaag
    15961 ctgttgcaga taaagatcaa gtgaaacgta cagaagatta tgcggatgca gatacagcta
    16021 aacaaaatgc atataacagt gcagtttcaa gtgccgaaac aatcattaat caaacaacaa
    16081 atccaacgat gtctgttgat gatgttaatc gtgcaacttc agctgttact tctaataaaa
    16141 atgcattaaa tggtgatgaa aaattagcac aatctaaaac agatgctgca agagcaattg
    16201 atgcattacc acatttaaat aatgcacaaa aagcagatgt taaatctaaa attaatgctg
    16261 catcaaatat tgctggcgta aatactgtta aacaacaagg tacagattta aatacagcga
    16321 tgggtaactt gcaaggtgca atcaatgatg aacaaacgac gcttaatagt caaaactatc
    16381 aagatgcgac acctagtaag aaaacagcat acacaaatgc ggtacaagct gcgaaagata
    16441 ttttaaataa atcaaatggt caaaataaaa cgaaagatca agttactgaa gcgatgaatc
    16501 aattgaattc ggctaaaaat aacttagatg gtacgcgttt attagatcaa gcgaagcaaa
    16561 cagcgaaaca gcagttaaat aatatgacgc atttaacaac tgcacaaaaa acgaatttaa
    16621 caaaccaaat taatagtggt actactgtcg ctggtgttca tacggttcaa tcaaatgcca
    16681 atacattaga tcaagccatg aatacgttaa gacaaagtat tgccaacaaa gatgcgacta
    16741 aagcaagtga agattacgta gatgctaata atgataagca aacagcatat aacaacgcag
    16801 tagctgctgc tgaaacgatt attaatgcaa atagtaatcc agaaatgaat ccaagtacga
    16861 ttacacaaaa agcagagcaa gtgaatagtt ctaaaacggc acttaacggt gatgaaaact
    16921 tagctacggc aaaacaaaat gcgaaaacgt acttaaacac attaacgagt attacagatg
    16981 ctcaaaagaa caatttgatt agtcaaatta gtagtgcgac aagagttagt ggtgttgata
    17041 ctgtaaaaca aaatgcacaa catctagatc aagctatggc tagcttgcaa agtggtatta
    17101 acaacgaatc tcaagttaaa tcatctgaga aatatcgtga tgctgataca aataaacaac
    17161 aagagtatga taatgctatt actgcagcga aagcgatttt aaataaacaa catggtccaa
    17221 acactgcgca aaatgcagtt gaagcagcat tgcaacgtgt gaatactgcg aaagatgcat
    17281 tgaatggcga tgcaaaatta attgcagctc aaaacgcagc gaaacaacat ttaggtactt
    17341 taacgcatat cactacagct caacgtaatg atttaacaaa tcaaatttca caagctacaa
    17401 acttagctgg tgttgaatct gttaaacaaa atgcgaatag tttagatggt gctatgggta
    17461 acttacaaac ggctatcaac gataagtcag gaacattagc gagccaaaac ttcttggatg
    17521 ctgatgagca aaaacgtaat gcatacaatc aagctgtatc agcagccgaa accattttaa
    17581 ataaacaaac tggaccgaat acagcgaaaa cagcagtcga acaagcactt aataatgtta
    17641 ataatgcgaa acatgcatta aatggtacgc aaaacttaaa caatgcgaaa caagcagcga
    17701 ttacagcaat caatggcgca tctgatttaa atcaacatca aaaagatgca ttaaaagcac
    17761 aagctaatgg tgctcaacgc gtatctaatg cacaagatgt acaacgtaat gcgactgaac
    17821 tgaacacggc gatgggcaca ttaaaacatg ccatcgcaga taagacgaat acgttagcaa
    17881 gcagtaaata tgtcaacgcc gatagcacta aacaaaatgc ttacacaact aaagttacca
    17941 atgctgaaca tattattagc ggtacgccaa cggttgttac gacaccttca gaagtaacag
    18001 ctgcagctaa tcaagtaaac agcgcgaaac aagaattaaa tggtgacgaa agattacgtg
    18061 tagcaaaaca aaacgccaat actgctattg atgcattaac gcaattaaat actcctcaaa
    18121 aagctaaatt aaaagaacaa gtgggacaag ccaatagatt agaagacgta caatctgttc
    18181 aaacaaatgg acaagcattg aacaatgcaa tgaaaggctt aagagatagt attgctaacg
    18241 aaacaacagt caaagcaagt caaaactata cagacgcaag tccgaataac caatcaacat
    18301 ataatagcgc tgtgtcaaat gcgaaaggta tcattaatca aactaacaat ccgactatgg
    18361 atgctagtgc gattacccaa gctgcaacac aagtgaataa tgctaaaaat ggtttaaacg
    18421 gtgctgaaaa cttaagaaat gcacaaaaca ctgctaagca aaacttaaat acgttatcac
    18481 acttaacaaa taaccaaaaa tctgccatct catcacaaat tgaccgtgca ggtcatgtga
    18541 gtgaggtaac agctgctaaa aatgcagcaa ctgagttaaa cacgcaaatg ggcaacttgg
    18601 aacaagctat ccatgatcaa aacacagtta aacaaggtgt taacttcact gatgcagata
    18661 aagctaaacg tgatgcgtat acaaatgcgg taagcagagc tgaagcaatt ctgaataaaa
    18721 cgcaaggtgc aaatacgtct aaacaagatg ttgaagcggc tattcaaaat gtttcaagtg
    18781 ctaaaaatgc attgaatggt gatcaaaacg ttacaaatgc gaagaatgca gctaaaaatg
    18841 cattaaataa cttaacgtca attaataatg cacaaaaacg tgacttaaca actaaaattg
    18901 atcaagcaac aactgtagct ggtgttgaag ctgtatctaa tacaggtaca caattgaata
    18961 cagcgatggc taacttgcaa aatggtatta atgataaagc aaatacacta gcaagtgaaa
    19021 actatcatga tgctgattca gataagaaaa ctgcttatac tcaagccgtt gcgaacgcag
    19081 aaaatatttt aaataaaaat agtggatcaa atttagataa agctgccgtt gaaaacgcgt
    19141 tgtcacaagt tgctaatgcg aaaggtgccc taaatggtaa ccataattta gagcaagcta
    19201 aatcaaatgc aaacactact ataaacggac ttcaacattt aacaactgct caaaaagata
    19261 aattgaaaca acaagtgcaa caagcacaaa atgttgcagg tgtagatact gttaaatcaa
    19321 gtgccaacac attaaatggt gctatgggta cgttaagaaa tagcattcaa gataacacag
    19381 ctacgaaaaa tggccaaaac tatcttgatg ctacaggaag taacaaaaca aactataaca
    19441 atgctgttga tagtgctaat ggtgtcatta atgcaacaag caatccaaat atggatgcta
    19501 atgcaattaa ccaaatcgct acacaagtga catcaacgaa aaatgcatta gatggtacac
    19561 ataatttaac gcaagcgaaa caaacagcaa caaatgccat cgatggtgct actaacttaa
    19621 ataaagcgca aaaagatgcg ttgaaagcac aagtgacaag tgcgcaacgt gttgcaaatg
    19681 taacaagtat ccaacaaact gcaaatgaac ttaatacagc tatgggtcaa ttacaacatg
    19741 gtattgatga tgaaaatgca acaaaacaaa ctcaaaaata tcgtgacgct gaacaaagta
    19801 agaaaactgc ttatgatcaa gctgtagctg ctgcgaaagc aattttaaat aaacaaacag
    19861 gttcaaattc agataaagca gcagttgacc gtgcattaca acaagtaaca agtacgaaag
    19921 atgcattgaa tggtgatgca aaactggcag aagcgaaagc ggcagctaaa caaaacttag
    19981 gcactttaaa ccatattacg aatgcacaac gtactgcgtt agaaggtcaa atcaatcaag
    20041 cgacgactgt tgatggcgtt aatactgtaa aaacaaatgc caatacatta gacgacgcaa
    20101 tgaatagctt acaaggtgca atcaatgata aagatgctac attaagaaat caaaattatc
    20161 ttgatgcaga tgaatcaaaa cgaaatgcat atacgcaagc tgtcacagcg gctgaaggca
    20221 ttttaaataa acaaactggt ggtaacacat ctaaagcaga cgttgataat gcattaaatg
    20281 cagttacaag agcgaaagcg gctttaaatg gtgctgaaaa cttaagaaat gcgaaaactt
    20341 cagcaacaaa tacgattaat ggtttaccta acttaacaca attacaaaaa gacaacttga
    20401 agcatcaagt tgaacaagcg caaaatgtag caggtgtaaa tggtgttaaa gataaaggta
    20461 ataccttaaa tactgccatg ggtgcattac gtacaagtat ccaaaatgat aatacgacga
    20521 aaacaagtca aaattatctt gatgcatctg atagcaacaa aaataattac aatactgctg
    20581 taaataatgc aaatggtgtt attaatgcaa cgaacaatcc aaatatggat gctaatgcga
    20641 ttaatggcat ggcaaatcaa gtcaatacaa caaaagcagc gttaaatggt gtacaaaact
    20701 tagctcaagc taaaacaaat gcgacgaaca caattaacaa cgcacatgac ttaaacccaa
    20761 aacaaaaaga tgcattaaaa acacaagtta acaatgcaca acgtgtatct gatgcaaata
    20821 acgttcaaca tacagctact gaattgaacg gtgcgatgac agcacttaaa gcagctattg
    20881 ctgataaaga aagaacaaaa gcaagcggta attatgtcaa tgctgatcaa gaaaaacgtc
    20941 aagcgtatga ttcaaaagtg actaacgctg aaaatatcat taatggtaca ccgaatgcga
    21001 cattaacagt caatgatgta aatagtgcga cttcacaagt caatgcggct aaaacagcat
    21061 taaatggcga taacaactta cgtgttgcta aagaaaatgc taataataca attgatggct
    21121 tagcacaatt gaataatgca caaaaagcaa aattaaaaga acaagttcaa agtgcaacta
    21181 cattagaagg tgttcaaact gttaaaaata gttctcaaac gttgaataca gcgatgaaag
    21241 gcttaagaga tagtattgcg aatgaagcaa caattaaagc aggtcaaaac tacactgacg
    21301 caagtccaac taatcgtaac gagtacgaca gcgcagttac tgcagcaaaa gcaatcatta
    21361 atcaaacatc gaacccaacg atggaaccaa atactattac gcaagcaaca tcacaagtga
    21421 caactaaaga acatgcatta aatggtgcgc aaaacttagc tcaagctaag acaactgcga
    21481 aaaacaactt aaataactta acatcaatta acaatgcaca aaaagatgcg ttaacgcgta
    21541 gcattgatgg tgcaactaca gtagctggtg taaatcaaga aactgccaaa gcaacagaat
    21601 taaataacgc aatgcatagt ttacaaaatg gtatcaatga tgagacacaa acaaaacaaa
    21661 ctcagaaata cctagatgca gagccaaata agaaatcagc ttatgatcaa gctgtaaatg
    21721 cagcgaaagc aattttaaca aaagctagtg gtcaaaatgt agacaaagca gcagttgaac
    21781 aagcattaca aaatgtgaac agtacgaaga cggcgttgaa cggtgatgcg aaattaaatg
    21841 aagctaaagc tgctgcgaaa caaacgttag gtacattaac acacattaat aatgcacaac
    21901 gtaatgcgtt agataatgaa attacacaag caacaaatgt tgaaggtgtt aatacagtta
    21961 aagccaaagc gcaacaatta gatggtgcta tgggtcaatt agaaacatca attcgtgata
    22021 aagacacgac gttacaaagt caaaattatc aagatgctga tgatgctaaa cgaacggctt
    22081 attctcaagc agtaaatgca gcagcaacta ttttaaataa aacagctggc ggtaatacac
    22141 ctaaagcaga tgttgaaaga gcaatgcaag ctgttgcaca agcaaatact gcattaaacg
    22201 gtattcaaaa cttagaacgt gcgaaacagg ctgctaacac agcgattaca aacgcttcgg
    22261 acttaaatac aaaacaaaaa gaagcattaa aagcacaagt aacaagtgca ggacgcgtat
    22321 ctgcagcaaa tggtgttgaa catactgcga ctgaaataaa tactgcgatg acagctttaa
    22381 aacgtgctat tgctgataaa gctgacacaa aaactagtgg taactatgtc aatgctgatg
    22441 cgaataaacg tcaagcatat gatgaaaaag tgacagctgc cgaaagtatc gttaatggta
    22501 caccaacacc aacgttaaca ccatcagatg ttacaaatgc agcaacgcaa gtaacgaatg
    22561 cgaagacgca gttaaacggt aatcataatt tagaagtagc gaaacaaaat gctaacacag
    22621 caattgatgg tttaacttct ttaaatggtc cgcaaaaagc aaaacttaaa gaacaagtgg
    22681 gtcaagcgac gacgttgcca aatgttcaaa ctgttcgtga taatgcacaa acattaaaca
    22741 ctgcaatgaa aggtctacga gatagcattg cgaatgaagc aacgattaaa gcaggtcaaa
    22801 actacacaga tgcaagtcct aataatcgta gtgaatacga tagtgcagtc actgcagcaa
    22861 aagcaatcat tggtcaaaca actagtccat caatgaatgc gcaagaaatt aatcaagcga
    22921 aagaccaagt gacagctaaa caacaagcgt taaacggtca agaaaactta agaactgcgc
    22981 aaacaaatgc gaagcaacat ttgaatggct taagtgactt aactaatgct caaaaagaag
    23041 cggcgaaacg tcaaatcgag ggtgcaacgc atgttaatga agtaacacaa gcacaaaata
    23101 atgcggatgc attaaataca gctatgacga acttgaaaaa tggtattcaa gatcagaata
    23161 cgattaagca aggtgttaac ttcactgatg cagatgaagc gaaacgtaat gcatatacaa
    23221 atgcagtgac gcaagctgaa caaattttaa ataaagcaca aggtccaaat actgcaaaag
    23281 acaatgttga atctgcgtta caaaatgtac aacgtgctaa aaacgaattg aacggtaatc
    23341 aaaatgttgc gaatgctaag acaactgcga aaaatgcatt gaataactta acatcaatca
    23401 acaacgcaca aaaagaagcg ttgaaatcac aaattgaagg tgctacaaca gttgcaggtg
    23461 taaatcaagt gtctacaaca gcatctgaat taaatactgc aatgagcaac ttacaacgtg
    23521 gtattaacga tgaagcagct acaaaagcag ctcagaaata tactgatgca gatagagata
    23581 aacaaactgc atacaatgat gctgtaacgg cagctaaaac gttattagat aaaacagctg
    23641 gcacaaatga aaataaagca gccgttgaac aagcattaca acgtgtgaat actgctaaaa
    23701 ctgcattaaa tggtgatgcg cgcttaaatg aagcgaagaa cacagctaaa caacaagtag
    23761 cgacaatgtc acacttaact gatgctcaaa aagcaaactt aacatcgcaa atcgaaagtg
    23821 gtacgactgt tgcaggtgtt caaggtattc aagctaatgc cggtacttta gatcaagcaa
    23881 tgaagcaatt aagacaaagt attgcttcta aagatgcgac taaatcaagc gaagattatc
    23941 aagacgcgaa tgcagattta caaaatgcat acaatgatgc ggtaactaat gctgaaggta
    24001 ttattagtgc aacgaataac cctgaaatga atcctgatac aattaaccaa aaagcgagcc
    24061 aagtgaacag tgcgaagtct gcattgaacg gtgatgaaaa attagcagca gcaaaacaaa
    24121 ctgcgaaatc agatatcggt cgtttgacag acttgaacaa tgcacaacga actgcggcaa
    24181 atgctgaagt ggatcaagca ccaaatcttg cagctgttac agcggctaaa aataaagcaa
    24241 catcgttaaa cacagcgatg ggtaatttga aacatgcact tgctgaaaag gataatacga
    24301 aacgtagtgt caattacaca gatgcggatc aaccaaaaca acaagcgtat gatactgcgg
    24361 ttacacaagc agaagcaatt actaatgcta atggcagcaa cgcgaatgaa acaacagttc
    24421 aagcagcact aaaccaattg aatcaagcta aaaatgactt gaatggtgat aataaagttg
    24481 ctcaagcaaa agagtcagcg aaacgtgcat tagcttcata tagtaacttg aataatgcgc
    24541 aatcaactgc agcaactagt caaattgaca atgcaacgac agtagcaggt gtaactgctg
    24601 cacaaaatac tgctaatgaa ttaaatacag caatgggtca acttcaaaat ggtattaatg
    24661 accaaaacac tgttaaacaa caagtgaact ttacagatgc tgaccaaggt aagaaagatg
    24721 cttacacaaa tgctgttacg aatgctcaag gtattttaga taaagcaaac ggtcaaaata
    24781 tgacaaaagc acaagttgaa gctgcattaa atcaagtaac gactgctaag aatgctttaa
    24841 acggtgatgc aaatgtaaga caagcaaaat cagatgcgaa agcaaattta ggtacattaa
    24901 cacacttaaa taatgcacaa aaacaagatt taacatcaca aatcgaaggt gcaacaacag
    24961 ttaacggtgt aaatagtgtt aaaacgaaag cacaagactt agatggtgca atgcaacgat
    25021 tagagtcagc aatcgcaaat aaagatcaaa ctaaagcgag cgaaaactac atcgacgcag
    25081 atccaactaa gaaaacagca tttgataatg ctatcacaca agctgaatct tacttaaata
    25141 aagatcatgg tacgaataaa gataagcaag ctgttgaaca agcaattcaa agtgtaacgt
    25201 ctactgaaaa tgctttgaac ggtgacgcga acttacaacg cgctaaaact gaagcaacac
    25261 aagctatcga taacttgaca catttgaata caccacaaaa aacagcatta aaacaacaag
    25321 tgaacgctgc gcaacgtgta tcaggtgtaa ctgatctgaa aaatagtgct acatcacttg
    25381 ataatgcgat ggatcaatta aaacaaggaa ttgctgatca tgacacaatt gttgctggtg
    25441 gtaattacac taacgcaagt cctgataagc aaggtgctta tactgatgca tataatgctg
    25501 cgaaaaacat tgtaaatggt tcacctaatg tgattacaaa tgcagcagat gttactgcgg
    25561 caacacaacg tgtcaataat gctgaaacaa gtttaaatgg tgattcaaac ttagcaactg
    25621 cgaagcaaca agctaaagat gcattacgtc aaatgacaca tttatctgat gcacaaaaac
    25681 aaagtattac tggtcaaatt gatagcgcga cacaagtaac tggtgtacaa agtgtgaaag
    25741 acaatgcaac aaatcttgac aatgcaatga atcaacttcg aaatagtatt gcgaataaag
    25801 atgaagtaaa agcgagtcaa ccatatgttg atgcagatac agataaacaa aatgcataca
    25861 atacagcagt tacaagtgct gaaaatatca ttaatgcaac gagtcagcca acacttgatc
    25921 catctgcagt aacacaagca gctaatcaag tgaacactaa caaaactgcg cttaatggtg
    25981 cgcaaaactt agcaaataaa aagcaagaaa caactgctaa catcaaccaa ttaagtcatt
    26041 taaataatgc tcaaaagcaa gatttaaata cacaagtgac aaatgcacca aatattagca
    26101 cagtaaatca agtgaaaact aaagctgaac aattagatca agcaatggaa cgtttaatca
    26161 acggaatcca agacaaagat caagtgaaac aaagtgttaa ctttacagat gcagatccag
    26221 aaaaacaaac agcatacaac aatgcggtaa ctgctgctga aaatattatt aatcaagcaa
    26281 atggtacaaa tgcgaaccaa tcacaagttg aagcagcact ttcaactgta acaactacta
    26341 aacaagcgtt gaatggtgat agaaaagtaa cagatgctaa aaacaatgca aaccaaacat
    26401 tatctacgtt agataactta aacaatgcac aaaaaggtgc tgttactgga aacatcaatc
    26461 aagcgcacac tgtagctgaa gtaacgcaag ccattcaaac cgctcaggaa ctgaatacag
    26521 cgatgggtaa cttgaaaaat agcttgaatg ataaagacac tacacttggc agtcaaaact
    26581 ttgcagatgc agatccagag aagaaaaatg catacaatga agcggttcgt aatgctgaaa
    26641 atattttaaa taaatctaca ggtacaaacg tgcctaaaga tcaagttgaa gcagctatga
    26701 atcaagtgaa tactacaaaa gcagcgctta atggtactca aaaccttgaa aaagctaaac
    26761 aacacgcaaa tacagcaatt gacggtttaa gccatttaac aaatgcacaa aaagaggcat
    26821 taaaacaatt ggtacaacaa tcgactactg ttgcagaagc acaaggtaat gaacaaaaag
    26881 caaacaatgt tgatgcagca atggacaaat tacgtcaaag tattgcagat aatgcgacaa
    26941 caaaacaaaa ccaaaattat actgatgcaa gtccgaataa aaaggatgcg tacaataatg
    27001 ctgtcacaac tgcacaaggt attattgatc aaactacaag tccaacttta gatccgactg
    27061 ttatcaatca agctgctgga caagtaagca caactaaaaa tgcattaaat ggtaatgaaa
    27121 acttagaggc agcgaagcaa caagcaacgc aatctttagg ttcattagac aacttaaata
    27181 atgcgcagaa acaagctgtt actgatcaaa ttaatggtgc gcatactgtt gatgaagcaa
    27241 atcaaattaa gcaaaatgcg caaaacttaa atactgcgat gggtaacttg aaacaagcga
    27301 tagccgataa agatgctacg aaagcaacag ttaacttcac tgatgcagat caagcaaaac
    27361 aacaagcata taacacagct gttacaaatg ctgaaaacat tatttcaaaa gctaatggtg
    27421 gcaatgcaac acaaactgaa gttgaacaag caatccaaca agtaaatgca gcaaaacaag
    27481 cattaaatgg taatgccaac gttcaacatg caaaagacga agcaacagca ttaattaata
    27541 gctctaatga ccttaaccaa gcacagaaaa atgcattaaa acaacaagtt caaaatgcaa
    27601 ctactgtagc tggtgtaaac aatgttaaac aaacagcaca agagttaaac aatgcgatga
    27661 cacaattaaa acaaggcatt gcagataaag aacaaacaaa agctgatggt aactttgtca
    27721 atgcagatcc tgacaagcaa aatgcatata atcaagcagt agcaaaagct gaagcattaa
    27781 ttagtggtac gcctgatgta gtcgttacac ctagcgaaat tactgcagct ttaaataaag
    27841 ttacgcaagc taaaaatgat ttaaatggta atacgaactt agcaacggcg aaacaaaatg
    27901 ttcaacatgc tattgatcaa ttgccaaact tgaatcaagc gcaacgtgat gaatacaaca
    27961 aacaaattac gcaagcaaca catgtaccaa acgtcaatgc tattcaacaa gcggcaacaa
    28021 cgcttaatga cgcgatgaca caattgaaac aaggtattgc gaataaagca caaattaaag
    28081 gtagcgagaa ctatcacgat gctgatactg acaagcaaac agcatatgat aatgcagtaa
    28141 caaaagcaga agaattgttg aaacaaacaa caaatccaac aatggatcca aatacaattc
    28201 aacaagcatt aactaaagtg aatgatacaa atcaagcact taacggtaat caaaaattag
    28261 ctgatgccaa acaagctgct aagacaaatc ttggtacatt agatcattta aatgatgcgc
    28321 aaaaacaagc gttaacaact caagttgaac aagcaccaga tattgcaaca gttaataatg
    28381 ttaagcaaaa tgctcaaaat ctgaataatg ctatgactaa cttaaacaat gcattacaag
    28441 ataaaactga gacattaaat agcattaact ttactgatgc agatcaagct aagaaagatg
    28501 cttatactaa tgcggtttca catgcagaag gtattttatc taaagcaaat ggcagcaatg
    28561 caagtcaaac tgaagtggaa caagcgatgc aacgtgtgaa cgaagcgaaa caagcattga
    28621 atggtaatga caatgtacaa cgtgcaaaag atgcagcgaa acaagtgatt acaaatgcaa
    28681 atgatttaaa tcaagcgcaa aaagatgcat taaaacaaca agtcgatgct gcgcaaactg
    28741 ttgcaaatgt aaacatgatt aagcaaacag cacaagattt aaatcaagca atgacacaat
    28801 tgaaacaagg tattgcagat aaagaccaaa ctaaagcaaa tggtaacttt gtcaatgctg
    28861 atactgataa gcaaaatgca tataacaatg cggtagcaca tgctgaacaa atcattagtg
    28921 gtacaccaaa tgcaaacgtg gatccacaac aagtggctca agcgttacaa caagtgaatc
    28981 aagctaaggg tgatttaaac ggtaaccata acttacaagt tgctaaagac aatgcaaata
    29041 cagccattga tcagttacca aacttaaatc aaccacaaaa aacagcatta aaagaccaag
    29101 tgtcgcatgc agaacttgtt acaggtgtta atgctattaa gcaaaatgct gatgcgttaa
    29161 ataatgcaat gggtacattg aaacaacaaa ttcaagcgaa tagtcaagta ccacagtcag
    29221 ttgactttac acaagcagat caagacaaac aacaagctta taacaatgca gctaaccaag
    29281 cgcaacaaat cgcaaatggc acaccaacac ctgtattggc gcctgataca gtaacacaag
    29341 cagttacaac tatgaatcaa gcgaaagatg cattaaacgg tgatgaaaaa ttagcgcaag
    29401 cgaaacaaga tgctttagca aatcttgata cgttacgtga cttaaatcaa ccacaacgtg
    29461 atgcattacg aaaccaaatc aatcaagcac aagctttagc tacagttgaa caaactaaac
    29521 aaaatgcaca aaatgtgaat acagcaatgg gtaacttgaa acaaggtatt gcaaataaag
    29581 atactgtgaa agcaagtgag aactaccacg atgctgatgt cgataagcaa acagcatata
    29641 caaatgcagt gtctcaagcg gaaggtatta tcaatcaaac gacaaatcca acgcttaacc
    29701 cagatgacat tactcgtgca ttaactcaag tgactgatgc taaaaatagc ttaaacggtg
    29761 aagctaaatt agccactgaa aagcaaaatg ctaaagatgc cgttaatgca atgacgcatt
    29821 taaacgatgc tcaaaaacaa gcattaaaag gtcaaatcga tcaatcgcct gaaattgcta
    29881 cagtgaacca agttaaacaa acagcaacga gcctagatca tgcaatggat caattatcac
    29941 aagctattaa tgataaagct caaacattag cggacggtaa ttacttaaat gcagatcctg
    30001 acaaacaaaa tgcgtataaa caggcagtag caaaagctga agcattattg aataaacaaa
    30061 gtggtactaa tgaagtacaa gcacaagttg aaagcatcac taatgaagtg aacgcagcga
    30121 aacaagcatt aaatggtaat gacaatttgg caaatgcaaa acaacaagca aaacaacaat
    30181 tggcgaactt aacacactta aatgatgcac aaaaacaatc atttgaaagt caaattacac
    30241 aagcgccact tgttacagat gtcactacga ttaatcaaaa agcacaaacg ttagatcatg
    30301 cgatggaatt attaagaaat agtgttgcgg ataatcaaac gacattagca tctgaagatt
    30361 atcatgatgc aactgcgcaa agacaaaatg actataacca agctgtaaca gctgctaata
    30421 atatcattaa tcaaactaca tcgcctacga tgaatccaga tgatgttaat ggtgcaacga
    30481 cacaagtgaa taatacgaaa gttgcattag atggtgatga aaaccttgca gcagctaagc
    30541 aacaagctaa caatagactt gatcaattag atcatttaaa taatgcgcaa aagcaacagt
    30601 tacaatcaca aattacgcaa tcatctgata ttgctgcagt taatggccac aagcaaacag
    30661 cagaaacttt aaatacagca atgggtaact taattaatgc gattgcagat catcaagccg
    30721 ttgaacaacg tggtaacttc atcaatgctg atactgataa acaaactgct tataatacag
    30781 cggtaaatga agcggcagca atgattaaca aacaaactgg tcaaaatgcg aaccaaacag
    30841 aagtagaaca agctattact aaagttcaaa caacacttca agcgttaaat ggagatcata
    30901 atttacaagt tgctaaaaca aatgcgacgc aagcaattga tgctttaaca agcttaaatg
    30961 atcctcaaaa aacagcatta aaagaccaag ttacagctgc aactttagta actgcagttc
    31021 atcaaattga acaaaatgcg aatacgctta accaagcaat gcatggtttg agacagagca
    31081 ttcaagataa cgcagcaact aaagcaaata gcaaatatat caacgaagat caaccagaga
    31141 aacaaaacta tgatcaagct gttcaagccg caaataatat tatcaatgaa caaactgcaa
    31201 cattagataa taatgcgatt aatcaagcag cggcaactgt gaatacaacg aaagcagcat
    31261 tacatggtga tgtgaaatta caaaatgata aagatcatgc taaacaaacg gttagtcaat
    31321 tagcacatct aaacaatgca caaaaacata tggaagatac gttaattgat agcgaaacaa
    31381 ctagaacagc agttaagcaa gatttgactg aagcacaagc attagatcaa cttatggatg
    31441 cattacaaca aagtattgct gacaaagatg caacacgtgc gagcagtgca tatgtcaatg
    31501 cagaaccgaa taaaaaacaa gcctatgatg aagcagttca aaatgctgag tctatcattg
    31561 caggattaaa taatccaact atcaataaag gtaatgtatc aagtgcgact caagcagtaa
    31621 catcatctaa aaatgcatta gatggtgttg aacgattagc tcaagataag caaactgctg
    31681 gaaattctct aaatcattta gatcaattaa caccagctca acaacaagcg ctagaaaatc
    31741 aaattaataa tgcaacaact cgtgataaag tggctgaaat cattgcacaa gcgcaagcat
    31801 taaatgaagc gataaaagca ttaaaagaaa gtattaagga tcaaccacaa actgaagcaa
    31861 gtagtaaatt tattaacgag gatcaagcgc aaaaagatgc ttatacgcaa gcagtacaac
    31921 acgcgaaaga tttgattaac aaaacaactg atcctacatt agccaaatca atcattgatc
    31981 aagcgacaca ggcagtgact gatgctaaaa acaatttaca tggtgatcaa aaactagctc
    32041 aagataagca acgtgcaaca gaaacgttaa ataacttgtc taacttgaat acaccacaac
    32101 gtcaagcact tgaaaatcaa atcaataatg cagcaactcg tgtcgaagta gcacaaaaat
    32161 taactgaagc acaagcactt aaccaagcaa tggaagcttt acgtaatagc attcaagatc
    32221 aacagcaaac ggaagcgggt agcaagttta tcaatgaaga taaaccacaa aaagatgctt
    32281 accaagcagc agttcaaaat gcaaaagatt taattaatca aactaacaat ccaacgcttg
    32341 ataaagcaca agttgaacaa ttgacacaag ctgttaacca agctaaagat aacctacacg
    32401 gtgatcaaaa acttgcagac gataaacaac atgcggttac tgatttaaat caattaaatg
    32461 gtttgaataa tccgcaacgt caagcacttg aaagccaaat aaacaacgca gcaactcgtg
    32521 atgaagtagc gcaaaaatta gctgaagcaa aagcgcttga tcaagcaatg caagcattac
    32581 gtaatagtat tcaagatcaa caacaaacag aatctggtag caagtttatc aatgaagata
    32641 aaccgcaaaa agatgcttac caagcagcag ttcaaaatgc aaaagattta attaaccaaa
    32701 caggtaatcc aacgcttgat aaagcacaag ttgaacaatt gacacaagca gtaacaactg
    32761 caaaagataa cctacatggt gatcaaaaac ttgctcgtga tcaacaacaa gcagtaacaa
    32821 ctgtaaatgc attgccaaac ataaatcatg cacaacaaca agcattaact gatgctataa
    32881 atgcagcgcc tacaagaaca gaggttgcac aacatgttca aactgctact gaacttgatc
    32941 acgcgatgga aacattgaaa aataaagttg atcaagtaaa tacagataag gctcaaccaa
    33001 attacactga agcgtcaact gataaaaaag aagcagtaga ccaagcgtta caagctgcag
    33061 aaagcattac agatccaact aatggttcaa atgcgaataa agacgctgta gaacaagcat
    33121 taactaagct tcaagaaaaa gtaaatgagt taaatggtga tgagagagtc gctgaagcta
    33181 aaacacaagc gaaacaaaat attgaccaat taacacattt aaatgctgat caaatcgcaa
    33241 ctgctaaaca aaacattgat caagcgacgc aacttcaacc aatcgctgaa ttagtagatc
    33301 aagcaacgca attgaaccaa tcaatggatc aattacaaca agctgttaat gatcatacaa
    33361 atgttgagca aacagttgat tacacgcaag cagattcaga taagcaaaag gcttataaac
    33421 aagcgattgc tgatgctgaa aatgtattga aacaaaatgc gaataagcaa caagtggatc
    33481 aagcacttca aaatatttta aatgcaaaac aagcattaaa tggtgatgaa cgtgtagcac
    33541 ttgctaaaac aaatggtaaa catgacatcg accaattgaa tgcattaaac aatgctcaac
    33601 aagatggatt taaaggtcgc atcgatcaat caaacgattt aaatcaaatc caacaaattg
    33661 tagatgaggc taaggcactt aatcgtgcaa tggatcaatt gtcagaagaa atcactggca
    33721 atgaaggacg cacgaaaggt agcacgaact atgtcaatgc agatacacaa gtcaaacaag
    33781 tatatgatga agcggttgat aaagcgaaac aagcacttga taaatcgact ggtcaaaact
    33841 taactgcaga acaagttatc aaattaaatg atgcagtaac ggcagcgaaa caggcattaa
    33901 atggtgaaga aagacttaat aatcgtaagt ctgaagcatt acaaagattg gatcaattaa
    33961 cacatctaaa caatgctcaa agacaatcag caatccaaca aattaataat gctgaaacgc
    34021 taaataaagc atctcgagca attaatagag caactaaatt agataatgca atgggtgcag
    34081 tacaacaata tattgacgaa cagcaccttg gtgttatcag cagcacaaat tacatcaatg
    34141 cagatgacaa tttgaaagca aattatgata atgcaattgc gaatgcagca catgagttag
    34201 ataaagtgca aggtaatgca attgcaaaag ctgaagcaga gcaattgaaa caaaatatta
    34261 tcgatgctca aaatgcatta aatggagacc aaaaccttgc aaatgccaaa gataaagcaa
    34321 atgcgtttgt taattcgtta aatggattaa atcaacagca acaagatctt gcacataaag
    34381 caattaacaa tgccgatact gtatcagatg taacagatat tgttaataat caaattgact
    34441 taaatgatgc aatggaaaca ttgaaacatt tagttgacaa tgaaattcca aatgcagagc
    34501 aaactgtcaa ttaccaaaac gctgacgata atgctaaaac aaacttcgat gatgccaaac
    34561 gtctagcaaa tgcattgcta aatagtgata acacaaatgt gaatgatatc aatggcgcaa
    34621 tccaagcagt caatgatgca atccataatc ttaatggtga tcaacgacta caagatgcta
    34681 aagacaaggc aattcaatct attaatcaag ctttagctaa taagctaaaa gaaatcgaag
    34741 cttcaaatgc gacggatcaa gacaagctta ttgcgaaaaa taaagcagaa gaattggcaa
    34801 acagcatcat caacaacatt aataaagcaa caagtaatca ggatgtatct caagttcaaa
    34861 cagctggcaa ccatgcgatt gaacaagtgc atgccaatga aataccgaaa gctaaaattg
    34921 atgcgaataa agatgttgac aagcaagttc aagcattaat tgacgaaatt gatcgaaatc
    34981 caaatctaac agataaggaa aaacaagcac ttaaagatcg tattaatcaa atacttcaac
    35041 aaggtcataa cgacattaac aatgcgctga ctaaagaaga aatcgaacaa gccaaagcac
    35101 aacttgcgca agcattacaa gaaattaaag atttagtgaa agctaaagaa aatgcgaaac
    35161 aagatgttga caagcaagtt caagctttaa ttgacgaaat cgatcaaaat ccaaatctaa
    35221 cagataagga aaaacaagca cttaaagatc gaattaacca aatacttcaa caaggtcata
    35281 acgacattaa caatgcgatg actaaagaag aaattgaaca agccaaagca caacttgcac
    35341 aagcattaca agacatcaaa gatttagtga aagctaaaga agatgcgaaa aatgcaataa
    35401 aagccttagc taatgcgaag cgtgatcaaa tcaattcaaa tccagattta acacctgagc
    35461 aaaaagcaaa agcgctcaaa gaaattgacg aagctgaaaa acgagcacta caaaacgttg
    35521 agaatgctca aactatagat caattaaatc gaggattaaa cttaggttta gatgacatta
    35581 gaaatacaca tgtatgggag gttgatgaac aacctgctgt aaatgaaatt tttgaagcaa
    35641 cacctgagca aatcctagtt aatggtgaac tcattgtaca tcgtgatgac atcattacag
    35701 aacaagatat tcttgcacac ataaacttaa ttgatcagct ttcagcagaa gttattgata
    35761 caccatcaac tgcaacgatt tctgatagct taacagcaaa agttgaagtt acattgcttg
    35821 atggatcaaa agtgattgtt aatgttcctg taaaagttgt agaaaaagaa ttgtcagtag
    35881 tcaaacaaca ggcaattgaa tcaatcgaaa atgcggcaca acaaaagatt aatgaaatca
    35941 ataatagtgt gacattaaca ctggaacaaa aagaagctgc aattgcagaa gttaataagc
    36001 ttaaacaaca agcaattgat catattaaca atgcacctga tgttcattca gttgaagaaa
    36061 ttcaacaaca agaacaagcg catattgaac aatttaatcc agaacaattt acgattgaac
    36121 aagcaaaatc aaatgcaatt aaatcgattg aagatgcaat tcaacatatg attgatgaaa
    36181 tcaaagctcg tactgatcta acagataaag agaagcaaga agctattgct aagttaaatc
    36241 aattaaaaga acaagcaatt caagcgattc aacgtgcgca aagcatcgat gaaataactg
    36301 agcaattgga acaatttaaa gctcaaatga aagcagctaa tccaacagca aaagaactag
    36361 ctaaacgcaa gcaagaagct attagtagaa ttaaagactt ttcaaatgaa aaaatgaata
    36421 gtattcgaaa tagtgaaatt ggcacagctg atgaaaaaca agcagcaatg aatcaaatta
    36481 acgaaattgt gcttgaaaca attagagata ttaataatgc gcatacatta cagcaagttg
    36541 aggctgcatt gaacaatggt attgctcgaa tttcagcagt acaaattgta atatctgatc
    36601 gtgctaaaca atcttcaagt actggaaatg aatctaatag ccatttaaca attggttatg
    36661 gaactgcaaa tcatccattt aacagttcga ctattggaca taaaaagaaa attgatgaag
    36721 atgatgacat tgatccactt catatgcgtc actttagtaa taatttcggt aatgttatta
    36781 aaaacgctat tggtgtggtg ggtatctctg gcttactagc tagtttctgg ttcttcattg
    36841 ccaaacgtcg tcgtaaagaa gatgaagagg aagaattaga aataagagat aataataaag
    36901 attcaataaa agagacttta gacgatacaa aacatttacc acttttattt gcgaaacgtc
    36961 gcagaaaaga agatgaagaa gatgttactg ttgaagaaaa agattcgcta aataatggcg
    37021 agtcactcga taaagttaaa catacgcctt tcttcttacc aaaacgtcgt cgtaaagaag
    37081 atgaagaaga tgtggaagtt acaaatgaaa acacagatga aaaagtgttg aaagataacg
    37141 aacattcacc actcttattc gcaaaacgac gcaaagataa agaggaagat gttgaaacaa
    37201 caactagtat tgaatctaaa gatgaggacg ttcctttatt attggctaaa aagaaaaatc
    37261 aaaaagataa ccaatccaaa gacaaaaagt cagcatcaaa aaatacttct aaaaaggtag
    37321 cagccaaaaa gaagaaaaag
//
LOCUS       DBNENW010000009        34597 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNENW010000009 DBNENW010000000
VERSION     DBNENW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.009"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(12..1394)
                     /locus_tag="AC1LW8_000324"
     CDS             complement(12..1394)
                     /locus_tag="AC1LW8_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIP4772193.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSRFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            1498..2385
                     /gene="pdxS"
                     /locus_tag="AC1LW8_000325"
     CDS             1498..2385
                     /gene="pdxS"
                     /locus_tag="AC1LW8_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIP4772194.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAKQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            2389..2949
                     /gene="pdxT"
                     /locus_tag="AC1LW8_000326"
     CDS             2389..2949
                     /gene="pdxT"
                     /locus_tag="AC1LW8_000326"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIP4772195.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3155..4369)
                     /locus_tag="AC1LW8_000327"
     CDS             complement(3155..4369)
                     /locus_tag="AC1LW8_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIP4772196.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            4527..4988
                     /locus_tag="AC1LW8_000328"
     CDS             4527..4988
                     /locus_tag="AC1LW8_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIP4772197.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5007..5573
                     /locus_tag="AC1LW8_000329"
     CDS             5007..5573
                     /locus_tag="AC1LW8_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIP4772198.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            5563..6570
                     /locus_tag="AC1LW8_000330"
     CDS             5563..6570
                     /locus_tag="AC1LW8_000330"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HIP4772199.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            6584..9040
                     /locus_tag="AC1LW8_000331"
     CDS             6584..9040
                     /locus_tag="AC1LW8_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIP4772200.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            9524..10888
                     /gene="radA"
                     /locus_tag="AC1LW8_000332"
     CDS             9524..10888
                     /gene="radA"
                     /locus_tag="AC1LW8_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIP4772201.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            10913..11986
                     /locus_tag="AC1LW8_000333"
     CDS             10913..11986
                     /locus_tag="AC1LW8_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIP4772202.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            12543..13997
                     /gene="gltX"
                     /locus_tag="AC1LW8_000334"
     CDS             12543..13997
                     /gene="gltX"
                     /locus_tag="AC1LW8_000334"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIP4772203.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14420..15067
                     /gene="cysE"
                     /locus_tag="AC1LW8_000335"
     CDS             14420..15067
                     /gene="cysE"
                     /locus_tag="AC1LW8_000335"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIP4772204.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15051..16451
                     /gene="cysS"
                     /locus_tag="AC1LW8_000336"
     CDS             15051..16451
                     /gene="cysS"
                     /locus_tag="AC1LW8_000336"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIP4772205.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDRGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16444..16848
                     /locus_tag="AC1LW8_000337"
     CDS             16444..16848
                     /locus_tag="AC1LW8_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIP4772206.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            16856..17602
                     /gene="rlmB"
                     /locus_tag="AC1LW8_000338"
     CDS             16856..17602
                     /gene="rlmB"
                     /locus_tag="AC1LW8_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIP4772207.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            17602..18126
                     /locus_tag="AC1LW8_000339"
     CDS             17602..18126
                     /locus_tag="AC1LW8_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIP4772208.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18207..18776
                     /locus_tag="AC1LW8_000340"
     CDS             18207..18776
                     /locus_tag="AC1LW8_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP4772209.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSVFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            18891..19034
                     /gene="rpmG"
                     /locus_tag="AC1LW8_000341"
     CDS             18891..19034
                     /gene="rpmG"
                     /locus_tag="AC1LW8_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP4772210.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19090..19272
                     /gene="secE"
                     /locus_tag="AC1LW8_000342"
     CDS             19090..19272
                     /gene="secE"
                     /locus_tag="AC1LW8_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIP4772211.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19285..19833
                     /gene="nusG"
                     /locus_tag="AC1LW8_000343"
     CDS             19285..19833
                     /gene="nusG"
                     /locus_tag="AC1LW8_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIP4772212.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            20014..20436
                     /gene="rplK"
                     /locus_tag="AC1LW8_000344"
     CDS             20014..20436
                     /gene="rplK"
                     /locus_tag="AC1LW8_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIP4772213.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            20644..21336
                     /gene="rplA"
                     /locus_tag="AC1LW8_000345"
     CDS             20644..21336
                     /gene="rplA"
                     /locus_tag="AC1LW8_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIP4772214.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    21424..21570
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            21608..22108
                     /gene="rplJ"
                     /locus_tag="AC1LW8_000346"
     CDS             21608..22108
                     /gene="rplJ"
                     /locus_tag="AC1LW8_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIP4772215.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEESAE"
     gene            22151..22519
                     /gene="rplL"
                     /locus_tag="AC1LW8_000347"
     CDS             22151..22519
                     /gene="rplL"
                     /locus_tag="AC1LW8_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIP4772216.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            22694..23302
                     /locus_tag="AC1LW8_000348"
     CDS             22694..23302
                     /locus_tag="AC1LW8_000348"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4772217.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKINGIDNAIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            23517..27068
                     /gene="rpoB"
                     /locus_tag="AC1LW8_000349"
     CDS             23517..27068
                     /gene="rpoB"
                     /locus_tag="AC1LW8_000349"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIP4772218.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            27232..30828
                     /gene="rpoC"
                     /locus_tag="AC1LW8_000350"
     CDS             27232..30828
                     /gene="rpoC"
                     /locus_tag="AC1LW8_000350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIP4772219.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            30965..31219
                     /locus_tag="AC1LW8_000351"
     CDS             30965..31219
                     /locus_tag="AC1LW8_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HIP4772220.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            31317..31730
                     /gene="rpsL"
                     /locus_tag="AC1LW8_000352"
     CDS             31317..31730
                     /gene="rpsL"
                     /locus_tag="AC1LW8_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIP4772221.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            31796..32266
                     /gene="rpsG"
                     /locus_tag="AC1LW8_000353"
     CDS             31796..32266
                     /gene="rpsG"
                     /locus_tag="AC1LW8_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIP4772222.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            32389..34470
                     /gene="fusA"
                     /locus_tag="AC1LW8_000354"
     CDS             32389..34470
                     /gene="fusA"
                     /locus_tag="AC1LW8_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIP4772223.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
BASE COUNT        12456 a         5062 c         6752 g        10327 t
ORIGIN      
        1 tcaaagaagt gttactttat aattaatgat tttattagag cgtctacatg cggttttaaa
       61 gcatcatcgt ctataccgcc aaagcctaat ataaatttag gggttttctt atagtcttga
      121 tcatcatcaa aattataaac ttgtaatttt aactttactt tgtttgctct atcaagacac
      181 tcttgtaatg ttaatccatt ttttactgta attgtaaaat gcatacccgt ttcagcacct
      241 tgaatatcaa gctgctcttt gtaaggtttc aatcttttta aaatataggt tagttttcta
      301 cgataaattc gtctcatttt atttaaatgc ctttcaaaac caccggaaga tataaacgtt
      361 gcaataaggt tttgcatatg aacaggtaca gtgttgcctt caatgtgatt ttgagaatga
      421 tattttttca ttatagaata gggtaacacc atatatgcaa ctcgacaact aggaaaaata
      481 gactttgaaa atgtactgat ataaatcact ttttctcctc ttgaatatag accttgaatt
      541 gctggaatgg gtttgccgaa atatctaaac tcggaatcat aatcatcttc tataataaat
      601 cgttcttctt tttcttgagc ccattgtatt aattgagttc gcttttttaa gtccatcaca
      661 tatccagttg gaaattgatg ggaaggcgtt atatatacta tatttttttg tgatttaata
      721 acttcatcta cgtttattcc attatcttca acttcaattt gttcatattc aacttgtttt
      781 ttatctaaaa tatttttgat tggtggataa ctaggttttt cgataataaa tcttgaagta
      841 taaagtaaat cgactaattg atttactaat tgttcggtag atgagccaat tataatttga
      901 ttaggatcac aaattacacc acgattagta aataaataaa atgccagttg aaatcttaaa
      961 tgtaattctc cttgaaaatg tcctctacgt aattgattta aatgatttgt atcataaaga
     1021 tctttggaat actttctgaa aagttctata gggaaatgtt tcgtatctat ttcatccaaa
     1081 ttaaaagcat aatcataagc ttcatcactc gcttttggtt tatatgaatc atcatcaaaa
     1141 agagagggga taggttgatt gtttaaaatt gttaaagatt caatttcgga cacaaaatat
     1201 ccagagcgag gtcttgaata aatgtaacct tcgtctaata gaagttgata tgcatgctct
     1261 acggttgttt ggctgataga taaatgtttg cttaattgtc ttttagaata aaatttatcg
     1321 ccttctttaa attgaccttc aattatttgt ttttttaatt tttcataaag ttgatggtat
     1381 aaagtgtttt tcaattttat aactgacctc ctaaatttat cttattttgt acctttttaa
     1441 atatcagttt atacattaca atgtatttaa tcaacttgaa aaggggtttt atgtataatg
     1501 agtaaaatta ttggatcaga cagagtcaaa agaggtatgg ctgaaatgca aaaaggcggc
     1561 gttattatgg atgtcgttaa tgctaagcaa gcaagaattg cagaagaagc aggcgcggta
     1621 gcagttatgg cattagaacg agtaccttct gatattagag ctgctggtgg cgttgcacgt
     1681 atggcaaacc ctaaaattgt agaagaagta atgaatgctg tttctattcc agtcatggct
     1741 aaagcacgta ttggtcatat cactgaagca agagtattag aggcgatggg tgttgactat
     1801 attgatgaat cagaagtgtt aacaccagca gatgaggaat atcacttaag aaaagatcaa
     1861 tttacagtac catttgtatg tggatgtcgt aatttaggtg aagctgcgcg tagaattggt
     1921 gaaggtgctg ctatgttacg tactaaaggt gaaccaggta caggtaatat tgttgaagct
     1981 gtaagacata tgagacaagt taattcagaa gttagtcgat tgactgtaat gaatgatgat
     2041 gagattatga cttttgcgaa agatatcggt gcgccttatg aaattttaaa acaaattaaa
     2101 gacaatggtc gtttaccggt agttaacttt gcagctggtg gcgttgcgac tcctcaagat
     2161 gctgctttaa tgatggaatt aggtgccgac ggtgtattcg ttggatcagg tatttttaaa
     2221 tcagaagatc cagaaaaatt tgctaaagca attgttcaag caacaacaca ttaccaagac
     2281 tatgagctaa ttggaagatt agcaagtgaa cttggcactg ctatgaaagg tttagatatc
     2341 aatcaattat cattagaaga acgtatgcaa gagcgtggtt ggtaagatat gaaaataggt
     2401 gtattagcat tacaaggtgc agtacgtgaa catattagac atattgaatt aagtggtcat
     2461 gaaggtattg cagttaaaaa agttgaacaa ttagaagaaa tcgagggctt aatattacct
     2521 ggtggcgagt ctacaacgtt acgtcgatta atgaatttat atggatttaa agaggcttta
     2581 caaaattcaa ctttacctat gtttggtaca tgcgcaggat taatagttct agcgcaagat
     2641 atagttggtg aagaaggata ccttaacaag ttgaatatta ctgtacaacg aaactcattc
     2701 ggtagacaag ttgacagctt tgaaacagaa ttagatatta aaggtatcgc tacagatatt
     2761 gaaggtgtct ttataagagc gccacatatt gaaaaagtag gccaaggcgt agatatccta
     2821 tgtaaggtta atgagaaaat tgtagccgtc cagcaaggta aatatttagg cgtatcattc
     2881 catcctgaat taacagatga ctatagagta actgattact ttattaatca tattgtaaaa
     2941 aaagcatagc ttaatgtatg ctaaatcaac gaattattga tatttataga tttgttgaga
     3001 agacaatatc tccttcaaac ttagctttgg aggagctatt tttttatgtc aaaattaaaa
     3061 atgataaaaa ataaagatat acataaaaaa acccttcaaa gagactgaga atagtcaaaa
     3121 ttttgaaggg gttaattcga tgttgatgta tttgttaaat aaagaatcca gcgattgcag
     3181 ctgaaatgaa agatactagt gttgcaccga ataatagttt caaaccaaag cgggcaactg
     3241 tatctccttt tttgtcatta agtgatttaa tcgcacctga aataatacca atagagctaa
     3301 agttagcaaa tgatactaag aatacagatg taacaccttt tgcgtgttca gataaatcac
     3361 taagtttacc aagtgcttgc attgctacaa attcgttaga taatagtttt gtcgccataa
     3421 ctgaaccggc ttgaactgca tcttgccatg gcacaccgac taagaatgca aatggtgcaa
     3481 agacaaaacc aattaatgtt tggaaatccc aagaaatagc gccacctgaa actgtactaa
     3541 agatattgct tacaattcca tttaatagag cgataatagc aatgtatccg attaacattg
     3601 cgcctacaat gacagctact ttaaatccat ctaaaatata ttctcctagc atttcgaaga
     3661 atgattgttg tctttcttca gtttcttcaa ctaataattt gtcatcttct tcattaactt
     3721 tataagggtt aataattgaa gcgatgatga aaccaccaaa taagtttaag acaacagccg
     3781 ttacaacata tttaggttca attaaggtaa agtatgcacc gataattgaa gcagaaacag
     3841 tcgacattgc tgaagctgtt aatgtgtata aacgttgctt aggtatgtat ggtaattgtt
     3901 ttttaattga aataaatact tcagattgtc ccaaaattgc tgcagcaact gcattgtatg
     3961 attctaaacg tcccatacca ttaattttag aaattaagaa tcctaaaaca ttaatgatta
     4021 aaggtaaaat ctttgtgtat tgaaggatac cgataatcgc tgaaataaat acgataggta
     4081 ataatacact gaagaagaat ggtggttgct taggatcgat atattgaata ccaccgaata
     4141 caaagttaac accatctgct gcttttaata ataagtagtt aaaaccgttt gaaataccac
     4201 caataacctt gattcccatt gtagttttaa gcaagataaa tgcaaagata agctgaattg
     4261 caagtaaaat tcctacatat ttccagcgaa tatttttcct gtctgagcta aatagaaacg
     4321 caagtgctaa aaagaagata attccgataa tcccaattag aatatgcata tatttctcat
     4381 tcctttagtt ttttctacaa tctatcatac aataaaatgg aagggctaac atcataaatt
     4441 tttgaaaata taaaaacaaa ttaattgaaa aaggtcaaaa taggtcatat aatatagtca
     4501 aagaaggtca aaaaggggtg atatacatgc acaatatgtc tgacatcata gaacaataca
     4561 tcaaacgttt atttgaagag tcgaatgaag atgtcgttga aattcagaga gcgaatatcg
     4621 cacagcgttt tgattgtgta ccatcacaat taaattatgt aatcaaaaca cgattcacta
     4681 atgaacatgg ttatgaaatc gaaagtaaac gtggtggtgg tggttacatc cgaatcacta
     4741 aaattgaaaa taaagatgca acaggttata ttaatcattt gcttcagctg attggacctt
     4801 ctatttctca acaacaagct tattatatta ttgatgggct tttagataaa atgttaataa
     4861 atgaacgtga agctaaaatg attcaagcag ttattgatag agaaacgcta tcaatggata
     4921 tggtttctag agatattatt agagcaaata ttttaaaacg tttgttacca gttataaatt
     4981 attactaaat gaaatgaggt gttgaagtgc tttgtgaaaa ttgtcaactt aatgaagcgg
     5041 aattaaaagt taaagttaca agtaaaaata aaacagaaga aaaaatggtg tgtcaaactt
     5101 gtgctgaggg gcaccatccg tggaatcaag ctaatgaaca acctgaatat caagaacatc
     5161 aagataattt cgaagaagca tttgttgtta agcaaatttt acaacattta gctacgaaac
     5221 atggcattaa ttttcaagaa gtagcgttta aagaagaaaa acgttgccca tcatgtcata
     5281 tgactttgaa agatattgca catgttggta aatttgggtg tgctaattgt tatgcaacat
     5341 ttaaagatga catcattgat atcgtccgca gagttcaagg tggacaattt gagcacgttg
     5401 gaaagacacc acattcttca cataaaaaga tagctttaaa gcgaaaaatc gaagaaaaga
     5461 atgaatattt gaaaaaactt attgaaatcc aagattttga ggaagcagcc attgttagag
     5521 atgaaattaa agcactaaaa gctgagagtg aggtgcaaca tgatgacgca taatattcat
     5581 gataatatca gccaatggat gaaaagtaat gaagaaacac caattgttat gtcttctaga
     5641 attcggttag cgcgtaattt agaaaatcat gtgcatccac taatgtatgc tactgaaaat
     5701 gatggattta gagttataaa tgaggtacaa gatgccttgc caaattttga attaatgcgt
     5761 cttgatcaaa tggatcaaca aagtaaaatg aaaatggttg caaagcattt gattagtcct
     5821 gaactaataa aacaaccagc agccgcagta ttagtgaatg atgatgaatc tttaagtgtc
     5881 atgataaatg aagaggacca tattcgtatt caagctatgg gaactgacac gacattacag
     5941 gctttatata atcaagcttc atcaattgat gatgaattag atcgaagcct tgatataagt
     6001 tatgatgaac aacttggtta tttaactaca tgtcctacca atataggtac tggtatgaga
     6061 gcaagcgtga tgctacattt accaggtcta tctattatga aaagaatgac acggattgct
     6121 caaaccatta atcgttttgg atatacaatc agaggtattt acggtgaagg ttcgcaagtt
     6181 tatggacata cttatcaagt atccaaccaa cttacacttg gtaaatctga gttagaaatc
     6241 atagaaacat taacagaagt tgttaatcaa atcattcatg aagaaaaaca aatacgacaa
     6301 aagttagaca cttataatca attagaaaca caagaccgtg tttttcgctc gctaggtatt
     6361 ttacaaaact gtagaatgat aactatggaa gaggcttctt atagattaag cgaagttaaa
     6421 cttggtatag atttaaatta cattgaatta caaaacttta aatttaatga attgatggta
     6481 gctatacagt caccattttt attagatgaa gaagatgaca aatctgtaaa agaaaaacga
     6541 gcagatatac taagagaaca tataaagtag gaggtcatta tttatgttat ttggtagatt
     6601 aactgagcgt gcacagcgcg tattagcaca tgcacaagaa gaagcaattc gtttaaatca
     6661 ttcaaatata ggaacagaac acctattatt gggattaatg aaagaacctg aaggaattgc
     6721 tgcaaaagta ttagaaagtt ttaatatcac tgaagataaa gtaattgaag aagttgaaaa
     6781 attaatcgga catggtcaag atcatgttgg tacattgcat tatacaccta gagctaaaaa
     6841 agttattgaa ttatcgatgg atgaagctag aaaattacat cacaattttg ttggaacgga
     6901 acatatttta ttaggcttga ttcgtgaaaa tgaaggtgtt gcagcaagag tttttgcaaa
     6961 tctagattta aatattacta aagcacgtgc acaagttgtg aaagctttag gaaaccctga
     7021 aatgagtaat aaaaatgcac aagctagtaa gtcaaataat actccaactt tagatagttt
     7081 agctcgtgac ttaacagtca ttgccaaaga cggtacatta gatcctgtta taggacgtga
     7141 taaagaaatt acacgtgtaa ttgaagtatt aagtagacgt acgaaaaaca atcctgtact
     7201 tattggagag ccaggtgttg gtaaaactgc tattgctgaa ggtttagcgc aagccatagt
     7261 gaataatgag gtaccagaga cattaaaaga taagcgtgtt atgtctttag atatgggaac
     7321 agtagttgca ggtactaaat atcgtggtga atttgaagag cgtctgaaaa aggttatgga
     7381 agaaatccaa caagcaggta atgtcatcct atttattgat gagttgcata ctttagttgg
     7441 tgctggtggt gctgaaggtg ctatcgatgc ttcgaatatt ttgaaaccgg cattagcacg
     7501 tggtgaatta caatgtattg gtgctactac attagatgaa tatcgcaaaa atattgaaaa
     7561 agacgcggct ttagaacgtc gtttccaacc tgtacaagtt gatgaacctt cagtagtaga
     7621 tacagttgct attttaaaag gattaagaga tcgttacgaa gcacaccatc gtattaatat
     7681 ttcagacgaa gctattgaag cagctgttaa attaagtaac agatacgttt cagatcgttt
     7741 cttaccagat aaagcaattg atttaattga tgaagcaagt tctaaagtaa gacttaagag
     7801 tcatacgaca cctaataatt taaaagaaat tgaacaagaa attgaaaaag ttaaaaatga
     7861 aaaagatgct gcagtacatg ctcaagagtt tgaaaatgct gctaacctgc gtgataaaca
     7921 aacaaaactt gaaaagcaat atgaagaagc taaaaatgaa tggaagaatg cacaaaatgg
     7981 catgtcaact tcattgtcag aagaagatat tgctgaagtt attgcaggat ggacaggtat
     8041 cccattaact aaaatcaatg aaacagaatc tgaaaaactt cttagtctag aagatacatt
     8101 acatgagaga gttattgggc aaaaagatgc tgttaattca atcagtaaag cggttagacg
     8161 tgcccgtgca gggttaaaag atcctaaacg accaattggt agctttatct tccttggacc
     8221 aactggtgtt ggtaaaactg aattagctag agctttagct gaatcaatgt ttggcgatga
     8281 tgatgcgatg atccgtgtag acatgagtga atttatggaa aaacacgcag tgagccgatt
     8341 agttggtgct cctccaggat atgttggtca tgatgatggt ggacaattaa ctgaaaaagt
     8401 tagacgtaaa ccatattctg taattttatt tgatgaaatt gaaaaagctc atccagatgt
     8461 atttaatatt ctattacaag ttttagatga tggacatttg acagatacaa aaggacgtac
     8521 agttgatttc agaaatacaa ttatcataat gacatcaaac gttggggcac aagaattaca
     8581 agatcaacga tttgctggat tcggtggttc aagtgatgga caagattatg aaacaattcg
     8641 aaaaacgatg ttaaaagaat taaaaaattc attccgtcca gaatttttaa accgtgtaga
     8701 tgatatcatt gtattccata aactaacaaa agaagaatta aaagaaattg taacaatgat
     8761 ggttaataaa ttaacaaatc gattatctga acaaaacata aatattattg ttactgataa
     8821 agcgaaagac aaaatcgcag aagaaggata tgatccagaa tatggtgcaa gaccattaat
     8881 tagagcgata caaaaaacta tcgaagataa tttaagtgaa ttaatattag atggtaatca
     8941 aattgaaggt aagaaagtta cagtagatca tgatggtaaa gagtttaaat atgacattgc
     9001 tgaacaaact tcagaaacta aaacaccatc gcaagcataa ttataaaaca gtccaaaaca
     9061 aattaaagtt ttgggctgtt tttttagtag cattgaacta tagaaattcg tgaaagtatc
     9121 catcaacgaa acaatctata aaacaatcat caaaggatag ttaagaatta tatgtaacaa
     9181 gttaatgagc ctacagcgat agcataaagg tatgaatttt tataaaggtt ttttgtttga
     9241 agatactagc aacttacgtc aaaataaaat aggtctatat aatattgtga taaatctaga
     9301 gaaaagagtt tactaagaaa ttattaagat tttatctttg aaagataccg actatcaaca
     9361 tgatgtaagt ttattttata atattcataa aaaataaatc tggtaaaaca gtttgcgtta
     9421 gtagtcatgt taaaatacaa acaaatgtaa ctcacattta atttgtcata atgggaatgt
     9481 gcgtttaaaa tagatttgtt caaaggaaag tggaggtgca attttggcca agaaaaaagt
     9541 gatttttgaa tgtatggctt gtggttatca atctcctaaa tggatgggga aatgtcctaa
     9601 ttgtggcgct tggaatcaaa tggaggaaat tgttgaaaaa gcagccaatc ctaaacatgg
     9661 agttaaaacc aaggaattag caggtaaagt acaaaaatta aatagtatta aacatgaaac
     9721 aacgccgaga gtgttaacag attcagcaga attcaaccgt gtattaggtg gaggtattgt
     9781 gagcggatcg ttagtactta ttggtgggga tccaggtatt ggtaagtcaa cgttactttt
     9841 acaaatttgt gcatcgttat ctcaaaagaa aaaagtacta tatattactg gagaagaatc
     9901 gcttagtcag actaaattac gtgcagagcg attagatgaa gattcaagtg aattgcaagt
     9961 attagctgaa acagatcttg aagttattta tcaaacagta aaagaagaac aacctgattt
    10021 attagtagtg gattcgattc aaacaatata tcatcctgaa atcagctctg cgccaggttc
    10081 tgtttcacaa gttcgtgaaa gtacacaaag tttaatgaat attgctaaac aaatgaacat
    10141 tgcaactttt atagtgggtc atgtaacgaa agaaggtcaa attgctggcc caagattgct
    10201 agaacacatg gttgatactg tgctttattt tgaaggcgat gaacaccacg catatcgaat
    10261 tttgcgagct gttaaaaacc gttttggttc aacgaatgaa atgggaatct tcgaaatgaa
    10321 gcaaagtgga ttaaaaggtg taaataatcc atctgaaatg tttttagaag aacgttcaac
    10381 aaatgttcca ggttcaacaa ttgttgcaac catggaggga accagaccac ttttaataga
    10441 agttcaagcg ctggtaactc caacgacttt taacaatccg agacgaatgg caacagggat
    10501 tgatcataat cgattaagtt tgttgatggc tgttttggaa aagaaagaaa attatctatt
    10561 acaacaacaa gatgcttata tcaaagtagc tggcggtgta aagttaacgg agccagcagt
    10621 tgatttaagt gtaattgtag caactgcatc tagctttaaa gataaagctg tcgacggatt
    10681 agattgctat attggagaag ttggtttaac gggtgaggta cgtcgtgtat ctcggataga
    10741 acaacgcgtg caagaggctg caaaactagg tttcaaacgt gtaattattc ctaaaaataa
    10801 tataggcgga tggacatatc ctgaaggtat acaagtaata ggtgtaacta ctgtacatga
    10861 agcattgtca tttgctcttc attcataaaa catcaagaaa ggaggacatt gtgtgaatat
    10921 cgttaaacta atggttatta ttatttactt aattattggg agcgcattag gaataattat
    10981 tattcctgaa attgcaaatg atcttggatt acaaaactcc agctttttaa aaaatcacta
    11041 tgtagatggc attatcggta gtatttttat gttcttaatt tttggtgtat ttattagacg
    11101 agttactaac gctataaaag gtttagaaca ttttattatg cgtagaagtg ctgttgaaat
    11161 actattcgca acaataggtt taataatcgg attacttatt tctgttatgg tgtcgtttat
    11221 attagaatca attggtaact ctatttttaa tcatttcatt cctgtcataa ttacgatatt
    11281 actatgttat ttcggtttcc aatttggcct taaaaaacga gatgaaatgt taatgttttt
    11341 acctgagaat atagcgcgtt ccatgtcaca acatactaaa agtgctacgc caaaaattat
    11401 cgacacaagc gcaattattg atggtcgtat tttagaagtc attcgttgcg gttttatcga
    11461 tggcaatatt ttaattccac aaggtgttat taatgaatta caaattgttg cagattcaaa
    11521 tgacagtgtt aaacgtgaaa agggtaaaag aggcttagat attttaaatg aattgtatga
    11581 tttagactat cctacaaagg ttatacatcc aactaaaaca catagtgata ttgatacgat
    11641 gttattaaaa ctagcaaaac aatatcatgc aagtattata acgacagatt tcaacctaaa
    11701 taaagtttgt catgtacatg gtattaaagc attaaatgtt aatgatttat cagaagcaat
    11761 caaacctaat gtacatcaag gtgatcaact gcatatttta ctgacaaaaa tgggtaagga
    11821 gcctggtcag gcagtaggat atctagatga tggtacgatg gtggttgttg ataatgctaa
    11881 aaatcttatt ggcagtcatg tcaatttaga agtagtcagc ttattgcaaa catcttcagg
    11941 aagaattgtt tttgctaaaa aaatcgaaga tacagtatca ttataaaaac tgtagttgac
    12001 gaaaacaaaa tagatattaa aaggtatatg aaatggcgaa gtttgtaatt tgaagtataa
    12061 ctataaataa atgcaaaaca tatcagttat caaaaaatgc aaatagtgct aatatatcat
    12121 tgaaacaagt tcattaagtg ttaaatttta gatattaata aagttattct tatagcaatc
    12181 aagacataca aaattagaat caattttaac gtatttgaat agttaatttg aagtttaatt
    12241 tcacagtaca taatattcat ttttaagtcg ttgaaatgtt tatgtagtgc agtcttgatt
    12301 cttagggata gcttttttaa agtgttgaaa aaacaactgc tttctattta aaatttaagc
    12361 atattaccca gggaattatg ttatgattaa acaatgaaat tattttaagg tcatgttaaa
    12421 aagcattgtt tcgttttaac aataagacga gttagacatt tgttatgacg gtcgaagata
    12481 gcaaatgaac aatttatatg aactaacttg aaaatctgat atttaattta ggagtgaaaa
    12541 aaatgagcga tcgtataaga gtaagatatg caccaagtcc aactggttat cttcatattg
    12601 gtaatgcaag aacagcatta ttcaattact tgtatgctaa acattacaac ggagattttg
    12661 tgattcgaat tgaagatact gataaaaaac gtaatttaga agatggagaa acatcacaat
    12721 ttgataatct taaatggtta ggattagatt gggatgagtc tgtagataaa gacaatggct
    12781 acggaccata tcgtcaatct gaacgtcaac atatctacca accattaata gatcagttac
    12841 tagcagaaga taaagcatat aaatgctata tgacagaaga agaattagaa gctgaacgtg
    12901 aagcacaaat cgctcgtggt gaaatgcctc gctatggtgg acaacatgcg catttgactg
    12961 aagaacaacg tcaacaattt gaagcagaag gacgccaacc atcaattcgt ttccgagtac
    13021 ctcaaaacca aacgtattca tttgatgata tggtaaaagg aaatatttca tttgattcaa
    13081 atggtattgg tgactgggtt atcgtaaaaa aagatggcat tccaacgtac aattttgcag
    13141 tagctataga tgatcattac atgcaaattt cagatgtaat tcgtggtgat gatcatattt
    13201 caaacacgcc taaacaaatt atgatttatg aagcatttgg ctgggagcca cctcgttttg
    13261 gtcatatgtc attaattgtt aatgaagaac gtaaaaagtt aagtaaacgt gatgggcaaa
    13321 ttttacaatt tattgagcaa tatcgtgact taggttattt acctgaagcg ttatttaatt
    13381 ttattgcgtt attaggttgg tctcctgaag gtgaagaaga aatcttttct aaagaagaat
    13441 ttatcaaaat ctttgatgaa aagcgtttgt caaaatcacc agcatttttc gataagcaaa
    13501 aattagcatg ggttaataac caatatatga aacaaaaaga tactgaaaca gtattccaat
    13561 tagcattacc tcatttaatt aaagcaaatt tgattcctga ggtgccgtca gaagaggatt
    13621 tatcttgggg acgcaaatta attgcgcttt atcaaaaaga aatgagttat gccggtgaaa
    13681 ttgtaccttt atcagaaatg ttctttaaag aaatgccagc tcttggtgaa gaagaacaac
    13741 aagtgattaa tggagagcaa gtaccagagt taatgacgca cttattcagt aaattagaag
    13801 cacttgaacc atttgaagcg gctgaaatta aaaagacaat taaagaagtt caaaaagaaa
    13861 caggaataaa aggcaagcaa ttatttatgc ctattcgtgt tgctgtaaca ggccaaatgc
    13921 atggtcctga attaccaaat acaattgaag tacttggtaa agaaaaagtg ctaaaccgtt
    13981 taaaacaata taagtaatga aatagacaag atcaaggtag tatatacttg aattttataa
    14041 cgtaaccaca tatgataaag ttgtattaac aaattacaac aatatgaata aagtatttta
    14101 tataaaacga aggattagta attaaattta tacgatgcag agagtgtacg gttgctgtga
    14161 gtacaacgta gaaattaatg aatgcacctt cgtaaaatga attaaatata taatgagagt
    14221 gatgagcatt aagttgactt agtttccttg ataatttgga agcgcccgca atattattaa
    14281 tgttattcgc taaattcaga gtggaaccgt gcggaagcgc ctctaacaat acaatttgta
    14341 tgttagtggt gcttttttga tatttaattt cgcaggtact tcaattatgt atttacgtag
    14401 ttcaatcatt gaggaggaaa tgatcttgtt aaaaagaatg agagacgata taaaaatggt
    14461 atttgagcag gatccagcgg cacgttcaac attagaagtc attacaacgt atgcaggttt
    14521 acatgcagtt tggagtcatt tgattgcaca taagttatac aaccaaaaaa aatatgttgc
    14581 agcacgcgcg atatctcaaa tttcaagatt tttcacaggt atagaaatcc atccaggtgc
    14641 taaaattgga aagcgtctat ttatagatca tggtatgggc gttgtaatag gagaaacatg
    14701 tacaattggt gataatgtga caatctatca aggcgtgaca cttggtggga cagggaaaga
    14761 aagagggaaa agacacccag atataggaga caatgtttta atagcagccg gtgcgaaagt
    14821 tttaggaaat attaaaataa attcaaatgt aaatattggt gcaaattcag ttgttttaca
    14881 atcagttcca agttattcaa cggttgttgg tataccagga catattgtta agcaagatgg
    14941 tgttcgagtt ggaaaaacat ttgatcatcg ccatctacct gatccaattt atgaacaaat
    15001 taagcattta gaacgacaac ttgaaaagac taggaatgga gagattcaag atgattacat
    15061 tatataatac gcttacacgt caaaaagaag tgttcaagcc tatagaacca gggaaagtaa
    15121 aaatgtatgt atgtggtcct actgtatata actacattca tattggtaac gcaagaccag
    15181 caattaatta tgacgtagtg agacgttact ttgaatacca aggatataat gtagaatatg
    15241 tatcaaattt tacagacgta gatgataaat taattaaacg ttctcaagaa ttaaatcagt
    15301 ctgttcccga aattgcagaa aaatatatcg ctgcttttca tgaagatgtt ggtgcgttaa
    15361 atgttagaaa agcgacttca aatccaaggg taatggacca tatggatgac attattcaat
    15421 ttattaaaga tttggtggat cgaggttatg catatgaaag tggtggcgat gtttacttta
    15481 gaacacgtaa atttgaaggt tatggtaaat taagtcatca atccatagat gacttaaaag
    15541 tgggtgctcg tatagatgca ggagagcata aagaagatgc acttgatttt acattgtgga
    15601 aaaaagcgaa gcctggcgag attagttggg atagcccatt tggtgaaggt agaccaggat
    15661 ggcatataga atgttctgta atggcatttc atgagctagg acctacaatt gatatacatg
    15721 cgggtggttc agatttacaa tttccacatc atgaaaatga aatagcacaa tcagaagcac
    15781 ataatcatgc gccatttgct aattattgga tgcataatgg tttcattaat attgataatg
    15841 aaaaaatgag taaatcacta ggcaacttta ttttagttca cgatattatt aaagaagttg
    15901 atccagatgt actaagattc tttatgatta gcgtacatta tagaagccca attaactata
    15961 atctagaatt ggtagaatca gcacgtagtg gactagagcg tattcgcaat agttatcaat
    16021 taattgaaga gcgcgcacaa attgctacta atattgaaaa tcaacagaca tatattgatc
    16081 aaattgatgc gattttaaat cgttttgaaa cagttatgaa tgatgatttt aatacagcta
    16141 atgcaattac agcttggtat gatttagcaa aacttgcgaa taaatatgta ctagagaaca
    16201 caacatcaac agaagtaatt gataaattta aagcagttta tcaaattttc agcgatgttt
    16261 taggtgtacc gttaaaatct aaaaatgcag atgaattatt ggatgaagat gttgaaaaat
    16321 taatcgaaga gcgtaatgaa gcaaggaaaa acaaagattt tgcacgagca gatgaaattc
    16381 gagacatgct gaaatcacaa aacattatat tagaagacac acctcaaggg gttagattta
    16441 aacgtggata atcaacaaga taatcacatt aaattattga atccattgac cttagcatat
    16501 atgggagacg cagtcttaga tcaatatgta cgtacctata tcgttttaaa gcttaaaagt
    16561 aagcctaata aactacatca aatgtctaaa aaatatgtat ctgccaaaag tcaggcgcaa
    16621 acgttagaat atttaatgga gcaagaatgg tttacagacg aagaaatgga tattttgaag
    16681 cgagggcgta acgcgaaaag tcatactaaa gctaaaaaca ctgatgttca aacatatcgt
    16741 aaaagttcag cgatagaagc agtgataggt tttctttatt tagaaaaaag agaagaacga
    16801 ttagaggcat tattaaataa aataataaca atagtaaacg aaaggtagtg acgatgtgga
    16861 agatacggtt attgttggta ggcatgctgt tagagaagcg attattactg ggcatccgat
    16921 aaataagata ttgattcaag aaggtattaa aaagcaacaa attaatgaaa ttttaaaaaa
    16981 tgcaaaagat caaaaaatca ttgttcaaac tgtaccaaaa tctaaattag attttttagc
    17041 aaatgcacca catcagggtg ttgcagcgct tattgcacca tatgaatatg ctgacttcga
    17101 tcaattttta aaacagcaaa aagaaaaaga aggtttatcg acagtactta tattagacgg
    17161 cttagaagac ccgcataact tgggatcaat tttaagaaca gccgatgcaa cgggagttga
    17221 tggtgttatt attcctaaac gtcgttcagt tacactaacg caaacagttg caaaagcctc
    17281 aacaggtgca attgaacatg taccagttat tcgagtgaca aatttagcta aaactatcga
    17341 tgaactaaaa gataatggct tttgggtagc tggcactgaa gctaataatg caacagatta
    17401 tagaaatcta gaagcggaca tgtcattggc tattgtaatt ggtagcgaag gacagggtat
    17461 gagtcgccta gtaagtgata aatgcgattt ttatattaag attccaatgg ttggacatgt
    17521 aaacagtttg aatgcttcgg ttgcagcaag tttaatgatg tacgaagtat ttcgaaaaag
    17581 acatgatgtt ggagaaatat aatgaaagaa cgttacttaa tcattgatgg atacaatatg
    17641 ataggacaat caccaacgct aagcgccatt gcaaaagaga atttagaaga agctagaatg
    17701 caattaatag atgcaattgc aaattataat gcagttattt cagatgaaat tatttgtgtt
    17761 ttcgatgctt atgaccaatc gggtgttgaa agagaataca tgtatcatgg cgttaaaacg
    17821 atttttacca aggaaaaaga aacagctgat agtttcatag aacgttatgt ttatgaactt
    17881 tatgacaagc atactaagca tattacagtt gtaacaagtg atatgagtga gcaacatgct
    17941 atctttggat caggtgcata tagaatatca tctcgcgaaa tgtggagaga tttaaaagaa
    18001 aatgaaattg atgtgagtaa atcattagat gatataagtg aaaacaagcc aagaactcga
    18061 attccgttat cttctgaaat ccttgcagaa tttgaaaaaa tacgaagagg acatcataag
    18121 aaatgacatt tccgtcatct tgaaattttg aatgtaacaa tattaaacta accttaaatt
    18181 ttagatagaa ggggttagtt taatatttga aatacgattt gacaactcaa gacagtacaa
    18241 tcaaacgtaa caatgcaata aatgataaag acttcgaaaa gttagtaatg gatctacaac
    18301 cattaattat tcgacgcatc aaaacatttg gatttaatca ttatgattta gaagacttat
    18361 atcaagaaat acttatacgg atgtataggt cggtccaaac atttgatttt agtggagagc
    18421 agcctttcac aaattatgtt caatgtttaa ttacgtctgt aaagtatgat tatttgagaa
    18481 aatatttagc tacaaataaa agaatggata atttgattaa tgaatataga gttacgtatc
    18541 catgtgcaat aaagcgttat gatgttgaaa acaattattt gaataaatta gcaattaaag
    18601 agttgattcg tcagtttaag tatttgagtg tatttgaaaa agatgtcatg tatttaatgt
    18661 gtgaacaata taaaccgaga gaaattgctc aactgatgca tgtaaaagag aaagtgattt
    18721 ataatgccat acaacgatgt aaaaataaaa taaaacgtta tttcaaaatg atttgaaaag
    18781 cgccttagga cgtgaattga attagaacgt gttacttact gatggtttga catttgttat
    18841 aaattttatg tatagtatac tggtattata atgaataaag gtgaattatt gtgagaaaaa
    18901 tacctttaaa ttgtgaagct tgtggcaata gaaattataa tgttcctaag caagaaggct
    18961 cggcaacaag attaacctta aagaaatatt gtccaaaatg taacgcgcac acaattcata
    19021 aagaatcgaa ataaatacat tcgaaataat actttgataa tatgttcaaa ggatttggag
    19081 gttgagcaga tggctaaaaa agaaagtttc tttaaaggcg ttaagtctga aatggaaaaa
    19141 acaagttggc cgacgaaaga agagctattt aaatatactg taattgtagt ttctactgtt
    19201 atattcttct tagtcttttt ctatgcctta gatttaggaa ttacagcatt gaaaaattta
    19261 ttatttggtt agaggagtga agacatgtct gaagaagttg gcgcaaagcg ttggtatgca
    19321 gtgcatacat attctggata tgaaaataaa gttaaaaaga atttagaaaa aagagtagaa
    19381 tctatgaata tgactgaaca aatctttaga gtagtcatac cggaagaaga agaaactcaa
    19441 gtaaaagatg gcaaagctaa aacgactgtt aaaaaaacat tccctggata tgttttagtg
    19501 gaattaatca tgacagatga atcatggtat gtggtaagaa atacaccagg tgttactggt
    19561 tttgtaggtt ctgcaggtgc agggtctaag ccaaatccat tgttaccaga agaagttcgc
    19621 ttcatcttaa aacaaatggg tcttaaagaa aagactatcg atgttgaact cgaagttggc
    19681 gagcaagttc gtattaaatc aggtccattt gcgaatcaag ttggtgaagt tcaagaaatt
    19741 gaaacagata agtttaagct aacagtatta gtagatatgt ttggccgaga aacaccagta
    19801 gaagttgaat tcgatcaaat agaaaagctt taattaacaa ttaaagttat taaactaacc
    19861 aaaagataaa aaagagtatt gattttttaa ttagaaaagt gttaaaatta tgtggtcgcg
    19921 cttttagagc gcccatttcg tcacgaaatg ttaagagtgg gagggcaaaa ctgagccctg
    19981 tgaccacatc acgatatcaa ggaggtgcac atcgtggcta aaaaagtaga taaagttgtt
    20041 aaattacaaa ttcctgcagg taaagcgaat ccagcaccac cagttggtcc agcattaggt
    20101 caagcaggtg tgaacatcat gggattctgt aaagagttca atgcacgtac tcaagatcaa
    20161 gcaggtttaa ttattccggt agaaatcagt gtttatgaag atcgttcatt tacatttatt
    20221 acaaaaactc caccggctcc agtattactt aaaaaagcag ctggtattga aaaaggttca
    20281 ggcgaaccaa acaaaactaa agttgctaca gtaactaaag atcaagtacg cgaaattgct
    20341 aacagcaaaa tgcaagactt aaacgctgct gacgaagaag cagctatgcg tattatcgaa
    20401 ggtactgcac gtagtatggg tatcgttgta gaataatttt acgaatatta aatttgatta
    20461 catgatttaa acgatgaagc agataacaga gataataatg atgaattata aatataatct
    20521 gaatgactag attaatgatt gatttattca taagattaat tcttctgttg tctgctctta
    20581 acttgcatat agcaagtaat gtgggaggaa attccgctaa aaccactaaa ggaggaacta
    20641 taaatggcta aaaaaggtaa aaagtatcaa gaagcagcta gtaaagttga ccgtactcag
    20701 cactacagtg ttgaagaagc aattaaatta gctaaagaaa caagcattgc taactttgac
    20761 gcttctgttg aagttgcatt ccgtttagga attgatacac gtaaaaatga ccaacaaatc
    20821 cgtggtgcag ttgtattacc aaacggaact ggtaaatcac aaagtgtatt agtattcgct
    20881 aaaggtgaca aaattgctga agctgaagca gcaggtgctg actatgtagg tgaagcagaa
    20941 tacgttcaaa aaatccaaca aggttggttc gacttcgatg tagtagttgc tacaccagac
    21001 atgatgggtg aagttggtaa attaggtcgt gtattaggac caaaaggttt aatgccaaac
    21061 cctaaaactg gaactgtaac aatggatgtt aaaaaagctg ttgaagaaat caaagctggt
    21121 aaagtagaat atcgtgctga aaaagctggt atcgtacatg catcaattgg taaagtttca
    21181 tttactgatg aacaattaat tgaaaacttc aatactttac aagatgtatt agctaaagct
    21241 aaaccatcat ctgctaaagg tacatacttc aaatctgttg ctgtaactac aacaatgggt
    21301 cctggagtta aaattgatac tgcaagtttc aaataataaa taatataaac aattacaggc
    21361 tgaaagaaat atctttcagt ctgtaaaaat atattgacaa taagtaattt ccaagttata
    21421 ttacttattg tgattatttt acctaagaca gtaggagtta tttataactt aaaatttatc
    21481 ctgccgaggc taaaattgac ttgaacgtga tgatctatga tctttcaagc actttttgcc
    21541 gtgggtagaa agtgcttttt ttattaattt taaaaaaagc accaaaaatt taaatggagg
    21601 tgtctgaatg tctgctatca ttgaagctaa aaaacaacta gttgatgaaa ttgctgaggt
    21661 actatcaaat tcagtttcaa cagtaatcgt tgactaccgt ggattaacag tagctgaagt
    21721 tactgactta cgttcacaat tacgtgaagc tggtgttgag tataaagtat acaaaaacac
    21781 tatggtacgt cgtgcagctg aaaaagctgg tatcgaaggc ttagatgaat tcttaacagg
    21841 tcctactgct attgcaactt caagtgaaga tgctgtagct gcagcgaaag taatttctgg
    21901 atttgctaaa gatcatgaag cattagaaat taaatcaggc gttatggaag gcaatgttat
    21961 tacagcagaa gaagttaaaa ctgttggttc attaccttca cacgatggtc ttgtatctat
    22021 gcttttatca gtattacaag ctcctgtacg caacttcgct tatgcggtta aagctattgg
    22081 agaacaaaaa gaagaaagcg ctgaataatt tttagcgtaa aaaaattaaa aataatggag
    22141 gaattataaa atggctaatc atgaacaaat cattgaagcg attaaagaaa tgtcagtatt
    22201 agaattaaac gacttagtaa aagcaattga agaagaattt ggtgtaactg cagctgctcc
    22261 agtagcagta gcaggtgcag ctggtggcgc tgacgctgca gcagaaaaaa ctgaatttga
    22321 cgttgagtta acttcagctg gttcatctaa aatcaaagtt gttaaagctg ttaaagaagc
    22381 aactggttta ggattaaaag atgctaaaga attagtagac ggagctccta aagtaatcaa
    22441 agaagcttta cctaaagaag aagctgaaaa acttaaagaa caattagaag aagttggagc
    22501 tactgtagaa ttaaaataat tcaagtatct taaacttaat aatcaaagtt ttatagcaag
    22561 tattgctata atataatgat tctttgagaa gttaaaaccc cgttattttg ataacggggt
    22621 tttattcatt taaagactga gtgaaatgtt ataattataa tgacgagtta caaagtgaag
    22681 atgaggtgga ataatgagtc attattacga tgaagatcca agtgtaatta gcaatgaaca
    22741 acgtattcaa tatcaattaa accatcataa aattgattta ataactgata atggagtgtt
    22801 ttcgaaagat aaagtagatt atggttcaga tgttcttgtt caaacttttt taaaagcgca
    22861 tccacctggt ccaagtaagc gaattgccga tgttggttgt ggttacggac caattggttt
    22921 gatgattgct aaagtatcac cacatcattc aattacaatg ctagatgtta atcacagagc
    22981 gctagcctta gttgaaaaaa acaaaaaaat aaatggtatt gataatgcga tcgtaaagga
    23041 aagtgatgct ttgtctgctg tggaagacaa aagttttgat tttattttaa ccaatccacc
    23101 aataagagca gggaaagaaa ccgtgcatcg tatattcgag caagcattac atagattaga
    23161 ctcgaacggt gaactattcg ttgtaattca gaagaagcaa ggtatgccat ctgcaaagaa
    23221 aagaatgaat gaactttttg gaaatgtaga agtggtaaat aaagataaag gatattacat
    23281 tctgagaagt ataaaagctt gaaatgaaat ggatattctg ttatagttat ataatgtaaa
    23341 aatttatgtt caataagtgt gtacttttac gttaaataga taagttaatt aagaataaat
    23401 atagaatcga aaatggtgtc atcattagtg ttgccgtttt ctttttgtct ttttattaat
    23461 atgcttatgg tatttagcta aaagcggatc acataatttt tgaggggtga atctgtttgg
    23521 caggtcaagt tgtccaatat ggaagacatc gtaaacgtag aaactacgcg agaatttcag
    23581 aagtattaga attaccaaac ttaatagaaa ttcaaactaa atcttacgag tggttcctaa
    23641 gagaaggttt aatcgaaatg tttagagaca tttctccaat tgaagatttt actggtaatt
    23701 tgtcattaga gtttgtggat taccgtttag gagaaccaaa atatgattta gaagaatcta
    23761 aaaaccgtga cgctacttat gctgcacctc ttcgtgtaaa agtgcgtcta atcattaaag
    23821 aaacaggaga agttaaagaa caagaagtct ttatgggtga tttcccatta atgactgata
    23881 caggtacgtt cgttatcaat ggtgcagaac gtgtaatcgt atctcaatta gttcgttcac
    23941 catccgttta tttcaatgaa aaaatcgaca aaaatggtcg tgaaaactat gatgcaacaa
    24001 ttattccaaa ccgtggtgca tggttagaat atgaaacaga tgctaaagat gttgtatacg
    24061 tacgtattga tagaacacgt aaactaccat taacagtatt gttacgtgca ttaggtttct
    24121 caagcgacca agaaattgtt gaccttttag gtgacaatga atatttacgt aatactttag
    24181 agaaagacgg cactgaaaac actgaacaag cgttattaga aatctatgaa cgtttacgtc
    24241 caggtgaacc accaactgtt gaaaatgcta aaagtctatt gtattcacgt ttctttgatc
    24301 caaaacgcta tgacttagca agcgtgggtc gttataaaac aaacaaaaaa ttacatttaa
    24361 aacatcgttt atttaatcaa aaattagctg agccaattgt aaatactgaa actggtgaaa
    24421 ttgtagttga agaaggtaca gtgcttgatc gtcgtaaaat cgacgaaatc atggatgtac
    24481 ttgaatcaaa tgcaaacagc gaagtgtttg aattgcatgg tagcgttata gacgagccag
    24541 tagaaattca atcaattaaa gtatatgttc ctaacgatga tgaaggtcgt acgacaactg
    24601 taattggtaa tgctttccct gactcagaag ttaaatgcat tacaccagca gatatcattg
    24661 cttcaatgag ttacttcttt aacttattaa gcggtattgg atatacagat gatattgacc
    24721 atttaggtaa ccgtcgttta cgttctgtag gtgaattact acaaaaccaa ttccgtatcg
    24781 gtttatcaag aatggaaaga gttgtacgtg aaagaatgtc aattcaagat actgagtcta
    24841 tcacacctca acaattaatt aatattcgac ctgttattgc atctattaaa gaattctttg
    24901 gtagctctca attatcacaa ttcatggacc aagcaaatcc attagctgag ttaacgcata
    24961 aacgtcgtct atcagcatta ggacctggtg gtttaacacg tgaacgtgct caaatggaag
    25021 tacgtgacgt tcactactct cactatggcc gtatgtgtcc aattgaaaca cctgagggac
    25081 caaacattgg attgattaac tcattatcaa gttatgcacg tgtaaatgaa ttcggcttta
    25141 ttgaaacacc atatcgtaaa gttgatttag atacacatgc tatcactgat caaattgact
    25201 atttaacagc tgacgaagaa gatagctatg ttgtagcaca agcaaactct aaattagatg
    25261 aaaatggtcg tttcatggat gatgaagttg tatgtcgttt ccgtggtaac aacacagtta
    25321 tggctaaaga aaaaatggat tatatggatg tatcgccgaa gcaagttgtt tcagcagcga
    25381 cagcatgtat tccattctta gaaaatgatg actcaaaccg tgcattgatg ggtgcgaaca
    25441 tgcaacgtca agcagtgcct ttgatgaatc cagaagcacc atttgttggt acaggtatgg
    25501 aacacgttgc agcacgtgat tctggtgcag ctattacagc taagcacaga ggtcgtgttg
    25561 aacatgttga atctaatgaa attcttgtac gtcgtctagt tgaagagaac ggcgttgagc
    25621 atgaaggtga attagatcgc tatccattag ctaaatttaa acgttcaaac tcaggtacat
    25681 gttacaacca acgtccaatc gttgcagttg gagatgttgt tgagtataac gagattttag
    25741 cagatggacc atctatggaa ttaggagaaa tggcattagg tagaaacgta gtagttggtt
    25801 tcatgacttg ggacggttac aactatgagg atgccgttat catgagtgaa agacttgtga
    25861 aagatgacgt gtatacttct attcatattg aagagtatga atcagaagca cgtgatacta
    25921 agttaggacc tgaagaaatc acaagagata ttcctaatgt ttctgaaagt gcacttaaga
    25981 acttagacga tcgtggtatc gtttatattg gtgcagaagt aaaagatgga gatattttag
    26041 ttggtaaagt aacgcctaaa ggtgtaactg agttaactgc cgaagaaaga ttgttacatg
    26101 caatctttgg tgaaaaagca cgtgaagtta gagatacttc attacgtgta cctcacggcg
    26161 ctggcggtat cgttcttgat gtaaaagtat tcaatcgcga agaaggcgac gatacattat
    26221 cacctggtgt aaaccaatta gtacgtgtat acatcgttca aaaacgtaaa attcatgttg
    26281 gtgataagat gtgtggtcga catggtaaca aaggtgtcat ttctaagatt gttcctgaag
    26341 aagatatgcc ttacttacca gacggacgtc cgatcgatat catgttaaac cctcttggtg
    26401 taccatctcg tatgaacatc ggacaagtat tagagctaca cttaggtatg gctgctaaaa
    26461 atcttggtat tcacgttgca tcaccagtat ttgacggcgc aaacgatgac gatgtatggt
    26521 caacaattga agaagctggt atggctcgtg atggtaaaac tgtactttat gatggacgta
    26581 caggtgaacc attcgataac cgtatttcag taggtgtaat gtacatgttg aaacttgcgc
    26641 acatggttga tgataaatta catgcgcgtt caacaggacc atattcactt gttacacaac
    26701 aaccacttgg aggtaaagcg caattcggtg gacaacgttt cggtgagatg gaggtatggg
    26761 cacttgaagc atatggtgct gcatacacat tacaagaaat cttaacttac aaatccgatg
    26821 atacagtagg acgtgtgaaa acatacgagg ctattgttaa aggtgaaaac atctctagac
    26881 caagtgttcc agaatcattc cgagtattga tgaaagaatt acaaagttta ggtttagatg
    26941 taaaagttat ggatgagcaa gataatgaaa tcgaaatgac agacgttgat gacgatgatg
    27001 ttgtagaacg taaagtagat ttacaacaaa atgatgctcc tgaaacacaa aaagaagtta
    27061 ctgattaata cgcaatttac aaaacaggca aaaagatact aagctgaatt ttattgatga
    27121 ttcagtttag tactttaagc cattttaaat aaatgcaaat caatcaaata gcacagctaa
    27181 tctaaattga aggaggtagg ctccttgatt gatgtaaata atttccatta tatgaaaata
    27241 ggattggctt cacctgaaaa aatccgttct tggtcttttg gtgaagttaa aaaacctgaa
    27301 acaatcaact accgtacatt aaaacctgaa aaagatggtc tattctgtga aaggattttc
    27361 ggacctacaa aagactggga atgtagttgt ggtaaataca aacgtgttcg ctacaaaggc
    27421 atggtctgtg acagatgtgg agttgaagta actaaatcta aagtacgtcg tgaaagaatg
    27481 ggtcacattg aacttgctgc tccagtttct cacatttggt atttcaaagg tataccaagc
    27541 cgtatgggat tattacttga catgtcacca agagcattag aagaagttat ttactttgct
    27601 tcttatgttg ttgtagatcc aggtccaact ggtttagaaa agaaaacttt attatctgaa
    27661 gctgaattca gagattatta tgataaatac ccaggtcaat tcgttgcaaa aatgggtgca
    27721 gaaggtatta aagatttact tgaagagatt gatcttgacg aagaacttaa attgttacgc
    27781 gatgagttgg aatcagctac tggtcaaaga cttactcgtg caattaaacg tttagaagtt
    27841 gttgaatcat tccgtaattc aggtaacaaa ccttcatgga tgattttaga tgtacttcca
    27901 atcatcccac cagaaattcg tccaatggtt caattagatg gtggacgatt tgcaacaagt
    27961 gacttaaacg atttataccg tcgtgtaatt aatcgaaata atcgtttgaa acgtttatta
    28021 gatttaggtg cacctggtat catcgttcaa aacgaaaaac gtatgttaca agaagccgtt
    28081 gacgctttaa ttgataatgg tcgtcgtggt cgtccagtta ctggcccagg taaccgtcca
    28141 ttaaaatctt tatctcatat gttaaaaggt aaacaaggtc gtttccgtca aaacttactt
    28201 ggtaaacgtg ttgactattc aggacgttca gttattgcag taggtccaag cttgaaaatg
    28261 taccaatgtg gtttaccaaa agaaatggca cttgaactat ttaaaccatt cgtaatgaaa
    28321 gaattagttc aacgtgaaat tgcaactaac attaaaaatg cgaagagtaa aatcgaacgc
    28381 atggatgatg aagtttggga cgtattggaa gaagtaatta gagaacatcc tgtattactt
    28441 aaccgtgcac caacacttca tagacttggt attcaagcat ttgaaccaac tttagttgaa
    28501 ggtcgtgcga ttcgtctaca tccacttgta acaacagctt ataacgctga ctttgacggt
    28561 gaccaaatgg cggttcacgt tcctttatca aaagaggcac aagctgaagc aagaatgttg
    28621 atgttagcag cacaaaacat cttgaaccct aaagatggta aacctgtagt tacaccatca
    28681 caagatatgg tacttggtaa ctattacctt actttagaaa gaaaagatgc agtaaataca
    28741 ggcgcaatct ttaataatac aaatgaagta ttaaaagcat atgcaaatgg ctttgtacat
    28801 ttacacacta gaattggtgt acatgcaagt tcgttcaata atccaacatt tactgaagaa
    28861 caaaacaaaa agattcttgc tacgtcagta ggtaaaatta tattcaatga aatcattcca
    28921 gattcatttg cttatattaa tgaacctacg caagaaaact tagaaagaaa gacaccaaac
    28981 agatatttca tcgatcctac aactttaggt gaaggtggat taaaagaata ctttgaaaat
    29041 gaagaattaa ttgaaccttt caacaaaaaa ttcttaggta atattattgc agaagtattc
    29101 aacagattta gcatcactga tacatcaatg atgttagacc gtatgaaaga cttaggattc
    29161 aaattctcat ctaaagctgg tattacagta ggtgttgctg atatcgtagt attacctgat
    29221 aagcaacaaa tacttgatga gcatgaaaaa ttagtcgaca gaattacaaa acaattcaac
    29281 cgtggtttaa tcactgaaga agaaagatat aatgcagttg ttgaaatttg gacagatgca
    29341 aaagatcaaa ttcaaggtga attgatgcaa tcacttgata aaactaaccc aatcttcatg
    29401 atgagtgatt caggtgcccg tggtaacgca tctaacttta cacagttagc aggtatgcgt
    29461 ggattgatgg ccgcaccatc tggtaagatt atcgaattac caatcacatc ttcattccgt
    29521 gaaggtttaa cagtacttga atacttcatc tcaactcacg gtgcacgtaa aggtcttgcc
    29581 gatacagcac ttaaaacagc tgactcagga tatcttactc gtcgtcttgt tgacgtggca
    29641 caagatgtta ttgttcgtga agaagactgt ggtactgata gaggtttatt agtttctgat
    29701 attaaagaag gtacagaaat gattgaacca tttatcgaac gtattgaagg tcgttattct
    29761 aaagaaacaa ttcgtcatcc tgaaactgat gaaataatca ttcgtcctga tgaattaatt
    29821 acacctgaaa ttgctaagaa aattacagat gctggtattg aacaaatgta tattcgctca
    29881 gcatttactt gtaacgcacg acatggtgtt tgtgaaaaat gttacggtaa aaaccttgct
    29941 actggtgaaa aagttgaagt tggtgaagca gttggtacaa ttgcagccca atctatcggt
    30001 gaaccaggta cacagcttac aatgcgtaca ttccatacag gtggggtagc aggtagcgat
    30061 atcacacaag gtcttcctcg tattcaagag attttcgaag cacgtaaccc taaaggtcaa
    30121 gcggtaatta cggaaatcga aggtgtcgta gaagatatta aattagcaaa agatagacaa
    30181 caagaaattg ttgttaaagg tgctaatgaa acaagatcat accttgcttc aggtacttca
    30241 agaattattg tagaaatcgg tcaaccagtt caacgtggtg aagtattaac tgaaggttct
    30301 attgaaccta agaattactt atctgttgct ggattaaacg cgactgaaag ctacttatta
    30361 aaagaagtac aaaaagttta ccgtatgcaa ggtgtagaaa tcgacgataa acacgttgag
    30421 gttatggttc gacaaatgtt acgtaaagtt agaattatcg aagcaggtga tacgaagtta
    30481 ttaccaggtt cattagttga tattcataac tttacagatg caaatagaga agcatttaaa
    30541 caccgtaagc gtcctgcaac agctaaacca gtattacttg gtattactaa agcatcactt
    30601 gaaacagaaa gtttcttatc tgcagcatca ttccaagaaa caacaagagt tcttacagat
    30661 gcagcaatta aaggtaagcg tgatgactta ttaggtctta aagaaaacgt aattattggt
    30721 aagttaattc cagctggtac tggtatgaga cgttatagcg acgtaaaata cgaaaaaaca
    30781 gctaaaccag ttgcagaagt tgaatctcaa actgaagtaa cggaataaca agtatataac
    30841 agaggctaat gctttagcct cttgttattt ttatgtaaat tatttgattt aatgttgacg
    30901 aattctcttg ttcaatgtta atatattaaa ggttgatgca agcagaactt tggaggataa
    30961 attattgtct aaggaaaaag ttgcacgctt taacaaacaa cattttgtag ttggtcttaa
    31021 agaaacgctt aaagcgttaa agaaagatca agttacatct ttgattattg ctgaagacgt
    31081 tgaagtatat ttaatgactc gcgtgttaag ccaaatcaat cagaaaaata tacctgtatc
    31141 ttttttcaaa agcaaacatg ctttgggtaa acatgtaggt attaacgtca atgcgacaat
    31201 agtagcattg attaaatgag aattagtaag tgttttactt actaaatttt atttaaccta
    31261 aaaatgaacc acctggatgt gtgggattaa aaagtgaaga gaggaggaca tatcacatgc
    31321 caactattaa ccaattagta cgtaaaccaa gacaaagcaa aatcaaaaaa tcagattctc
    31381 cagctttaaa taaaggtttc aacagtaaaa agaaaaaatt tactgactta aactcaccac
    31441 aaaaacgtgg tgtatgtact cgtgtaggta caatgacacc taaaaaacct aactcagcgt
    31501 tacgtaaata tgcacgtgtg cgtttatcaa acaacatcga aattaacgca tacatccctg
    31561 gtatcggcca taacttacaa gaacacagtg ttgtacttgt acgtggtgga cgtgtaaaag
    31621 acttaccagg tgtgcgttac catattgtac gtggagcact tgatacttca ggtgttgacg
    31681 gacgtagaca aggtcgttca ttatacggaa ctaagaaacc taaaaactaa gaatttagtt
    31741 tttaattaaa tcttaaactt aaaatattta atataaggaa gggaggattt acattatgcc
    31801 tcgtaaagga tcagtaccta aaagagacgt attaccagat ccaattcata actctaagtt
    31861 agtaactaaa ttaattaaca aaattatgtt agatggtaaa cgtggaacag cacaaagaat
    31921 tctttattca gcattcgacc tagttgaaca acgcagtggt cgtgatgcat tagaagtatt
    31981 cgaagaagca atcaacaaca ttatgccagt attagaagtt aaagctcgtc gtgtaggtgg
    32041 ttctaactat caagtaccag tagaagttcg tccagagcgt cgtactactt taggtttacg
    32101 ttggttagtt aactatgcac gtcttcgtgg tgaaaaaacg atggaagatc gtttagctaa
    32161 cgaaatttta gatgcagcaa ataatacagg tggtgccgtt aagaaacgtg aggacactca
    32221 caaaatggct gaagcaaaca aagcatttgc tcattaccgt tggtaagata aaagctttta
    32281 ccctgagtgt gttctatatt aatgaatttt cattaagcgt tcatgcttag ggcatcgcca
    32341 tatctatcgt atttattcag taatataaac tggaaggaga aaaaatacat ggctagagaa
    32401 ttttcattag aaaaaactcg taatatcggt atcatggctc acattgatgc tggtaaaacg
    32461 actacgactg aacgtattct ttattacact ggccgtatcc acaaaattgg tgaaacacac
    32521 gaaggtgctt cacaaatgga ctggatggag caagaacaag accgtggtat tactatcaca
    32581 tctgctgcaa caacagcagc ttgggaaggt caccgtgtaa acattatcga tacacctgga
    32641 cacgtagact tcactgtaga agttgaacgt tcattacgtg tacttgacgg agcagttaca
    32701 gtacttgatg cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaggctaca
    32761 acttatggtg ttccacgtat cgtatttgta aacaaaatgg acaaattagg tgctaacttc
    32821 gaatactctg taagtacatt acatgatcgt ttacaagcta acgctgctcc aatccaatta
    32881 ccaattggtg cggaagacga attcgaagca atcattgact tagttgaaat gaaatgtttc
    32941 aaatatacaa atgatttagg tactgaaatt gaagaaattg aaattcctga agaccactta
    33001 gatagagctg aagaagctcg tgctagctta atcgaagcag ttgcagaaac tagcgacgaa
    33061 ttaatggaaa aatatcttgg tgacgaagaa atttcagttt ctgaattaaa agaagctatc
    33121 cgccaagcta ctactaacgt agaattctac ccagtacttt gtggtacagc tttcaaaaac
    33181 aaaggtgttc aattaatgct tgacgctgta attgattact taccttcacc actagacgtt
    33241 aaaccaatta ttggtcaccg tgctagcaac cctgaagaag aagtaatcgc gaaagcagac
    33301 gattcagctg aattcgctgc attagcgttc aaagttatga ctgaccctta tgttggtaaa
    33361 ttgacattct tccgtgtgta ttcaggtaca atgacatctg gttcatacgt taagaactct
    33421 actaaaggta aacgtgaacg tgtaggtcgt ttattacaaa tgcacgctaa ctcacgtcaa
    33481 gaaatcgata ctgtatactc tggagatatc gctgctgcgg taggtcttaa agatacaggt
    33541 actggtgata ctttatgtgg tgagaaaaat gacattatct tggaatcaat ggaattccct
    33601 gagccagtta ttcacttatc agtagagcca aaatctaaag ctgaccaaga taaaatgact
    33661 caagctttag ttaaattaca agaagaagac ccaacattcc atgcacacac tgacgaagaa
    33721 actggacaag ttatcatcgg tggtatgggt gagcttcact tagacatctt agtagaccgt
    33781 atgaagaaag aattcaacgt tgaatgtaac gtaggtgctc caatggtttc atatcgtgaa
    33841 acattcaaat catctgcaca agttcaaggt aaattctctc gtcaatctgg tggtcgtggt
    33901 caatacggtg atgttcacat tgaattcaca ccaaacgaaa caggcgcagg tttcgaattc
    33961 gaaaacgcta tcgttggtgg tgtagttcct cgtgaataca ttccatcagt agaagctggt
    34021 cttaaagatg ctatggaaaa tggtgtctta gcaggttatc ctttaattga tgttaaagct
    34081 aaattatatg atggttcata ccatgatgtc gattcatctg aaatggcctt caaaattgct
    34141 gcatcattag cacttaaaga agctgctaaa aaatgtgatc ctgtaatctt agaaccaatg
    34201 atgaaagtaa ctattgaaat gcctgaagag tacatgggtg atatcatggg tgacgtaaca
    34261 tctcgtcgtg gacgtgttga tggtatggaa cctcgtggta atgcacaagt tgttaatgct
    34321 tatgtaccac tttcagaaat gttcggttat gcaacatcat tacgttcaaa cactcaaggt
    34381 cgcggtactt acactatgta cttcgatcac tatgctgaag ttccaaaatc aatcgctgaa
    34441 gatattatca agaaaaataa aggtgaataa tataacttgt tttgactagc tagcctaggt
    34501 taaaatacaa ggtgagctta aatgtaagct atcatcttta tagtttgatt ttttggggtg
    34561 aatgcattat aaaagaattg taaaattctt tttgcat
//
LOCUS       DBNENW010000010        34121 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNENW010000010 DBNENW010000000
VERSION     DBNENW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.010"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..300)
                     /locus_tag="AC1LW8_000355"
     CDS             complement(<1..300)
                     /locus_tag="AC1LW8_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772224.1"
                     /translation="MEENKTLKEYLRKFLEGYKYVVENRYNYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNIDIKYLSKHFTSYIYDSKKYV"
     gene            complement(332..1057)
                     /locus_tag="AC1LW8_000356"
     CDS             complement(332..1057)
                     /locus_tag="AC1LW8_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP4772225.1"
                     /translation="MKELPKSRLTFKESMIESQYLATKTKEEKKQYKQLSVEDKREIL
                     KEYQSKPRKEVKFESEINKSDENLSKIYQRFSEIGVEDLFGTKKEVKELPMILKDNEN
                     IMYVTSGLYNNNTYLIVCTDLRLLFLDKGMIYGLKFHEFPFEKINSVSYKKGLLFGEI
                     IIHHGSSSIAIGSISKNTVSRMAETIQEQISIRESSMKPSNSEKMSFSVADELIKYKE
                     LLDVGVISQEEFDKKKQQLLDID"
     gene            complement(1085..1758)
                     /locus_tag="AC1LW8_000357"
                     /pseudo
     CDS             complement(1085..1758)
                     /locus_tag="AC1LW8_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(1775..2107)
                     /locus_tag="AC1LW8_000358"
     CDS             complement(1775..2107)
                     /locus_tag="AC1LW8_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4772226.1"
                     /translation="MNLVQRIRNLCNSKGMTFAELERTLGFSNGQIRRWEKTKPGIDK
                     VQKIADHFDVSVDYLLGREKDEYSGEDKSEDILIMHRATENMTEAQRQKALTILEAMF
                     DDWDDLTK"
     gene            2370..2564
                     /locus_tag="AC1LW8_000359"
     CDS             2370..2564
                     /locus_tag="AC1LW8_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4772227.1"
                     /translation="MSTTDFGLKVRTELLKRNMTNKQLAEMLEISSAYLSDILRGRRD
                     AFEQKKRIAKILEIKEEVKS"
     gene            2564..3331
                     /locus_tag="AC1LW8_000360"
     CDS             2564..3331
                     /locus_tag="AC1LW8_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HIP4772228.1"
                     /translation="MNEIKTFSNDMFSILIKQDNENNLFDLETVAKSLGFTQFKNGKQ
                     YIRWETINKYLGKYLSQEVGKGDFIPEPMVYKLAFKAGNAVAEKFQDWLAMEVLPAIR
                     KHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKS
                     TGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTDSDTITIVR
                     SDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA"
     gene            3332..3556
                     /gene="tscA"
                     /locus_tag="AC1LW8_000361"
     CDS             3332..3556
                     /gene="tscA"
                     /locus_tag="AC1LW8_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin TscA"
                     /protein_id="HIP4772229.1"
                     /translation="MTPEQKEKLNNIVLTLYAVKENKSQTYTHKDTLTVTYAGEIEHT
                     YEVDREKHLESMIEWAIDQIEQHFDLDEEE"
     gene            3596..3739
                     /locus_tag="AC1LW8_000362"
     CDS             3596..3739
                     /locus_tag="AC1LW8_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772230.1"
                     /translation="MLQKFRIAKEKSKLKLNLLKHANSNLETRNNPELLRAVAELLKE
                     INR"
     gene            complement(3761..4408)
                     /locus_tag="AC1LW8_000363"
     CDS             complement(3761..4408)
                     /locus_tag="AC1LW8_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772231.1"
                     /translation="MNNINLTQRQLDLIKKNKAILSKLPVEAYAKATNTMNNSYVMNA
                     LEIQSTVNNVMNSIRINQSKLSDWASYMHRVTKNHPMFKTNLFSEKILDEFISSNNFP
                     EDEVRKVSTHLRNSFVDTVDVPVLGKTVDSAHPIDDVNTKKYNSVFNESLNHIFISPS
                     AKFIKKVSVGSAIGVTSPVMVRTILDQYVNYFMFFNVIATLLTLYVIANYLDDEN"
     gene            4458..4595
                     /locus_tag="AC1LW8_000364"
     CDS             4458..4595
                     /locus_tag="AC1LW8_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772232.1"
                     /translation="MVEVPPHIQQMLYEIQLKAGIPQKLMEMQGLINDETTKEEKKEN
                     E"
     gene            4588..4749
                     /locus_tag="AC1LW8_000365"
     CDS             4588..4749
                     /locus_tag="AC1LW8_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HIP4772233.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFM
                     YYKECFFKE"
     gene            4841..5101
                     /locus_tag="AC1LW8_000366"
     CDS             4841..5101
                     /locus_tag="AC1LW8_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HIP4772234.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            5111..5332
                     /locus_tag="AC1LW8_000367"
     CDS             5111..5332
                     /locus_tag="AC1LW8_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HIP4772235.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDISYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            5325..6113
                     /locus_tag="AC1LW8_000368"
     CDS             5325..6113
                     /locus_tag="AC1LW8_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP4772236.1"
                     /translation="MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGK
                     TTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEI
                     NVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFV
                     ITGHEGINKDKDDEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKVR
                     YILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN"
     gene            6142..6693
                     /locus_tag="AC1LW8_000369"
     CDS             6142..6693
                     /locus_tag="AC1LW8_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP4772237.1"
                     /translation="MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDREN
                     RYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIKLNLPSLDFDTNDLIG
                     KFCHLVLKWKFNEDEGKYFTDFSFIKPYKKGDDVVNKPIPKTDKQKAEENNGAQQQTS
                     MSQQSNPFESSGQFGYDDQDLAF"
     gene            6706..7377
                     /locus_tag="AC1LW8_000370"
     CDS             6706..7377
                     /locus_tag="AC1LW8_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HIP4772238.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GEKKE"
     gene            complement(7483..8340)
                     /locus_tag="AC1LW8_000371"
     CDS             complement(7483..8340)
                     /locus_tag="AC1LW8_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4393 domain-containing protein"
                     /protein_id="HIP4772239.1"
                     /translation="MDPILGKGIDKIIEGASKGPVETFSKTWELVFGKFHLYVDKVIY
                     QREVEFEKFKEQFKKEISSVPENNLQEPQFSLLGPALEASKFYISEKTLSNMFAKLIA
                     SSMDDRKNSLTHHSFVEIIKQLSPNDAILLKHLKNHEVHPAVKYRAVLNPKNDGMNIS
                     DTLIKDSPLDIESTEISINNLVRLGVLNETFDMSYLTKKGIYNKFYAPQFLNHFNKII
                     EKQRFVSGLEFVKRMLKSGHNLETISKLSGIEFEVLKLHYSPWVIDIKKGSISLSAYG
                     KAFVKTCIN"
     gene            8404..9141
                     /locus_tag="AC1LW8_000372"
     CDS             8404..9141
                     /locus_tag="AC1LW8_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HIP4772240.1"
                     /translation="MTDQPSYYSIITANVRYDNRLTDSEKLLFAEITSLSNKYGYCTA
                     SNGYFAKLYEVTKVTVSRRIANLKECGYLHVEIIRNGNEIKQRKLYPLTEMIRPINTN
                     DNTPINNSVNTPIITNVKENNTSINNTSNNNINRVDILSGNPTRIPYKEIIDYLNEKT
                     GKKFKHNTTKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKYLRPETLFGNKF
                     EGYLNQKVQPTGTDQLERMKYDESYWD"
     gene            9151..9930
                     /locus_tag="AC1LW8_000373"
     CDS             9151..9930
                     /locus_tag="AC1LW8_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP4772241.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVNNYKPQNEKQVHA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            9924..10082
                     /locus_tag="AC1LW8_000374"
     CDS             9924..10082
                     /locus_tag="AC1LW8_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772242.1"
                     /translation="MVTKEFLKTKLECSDMYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAIVEY"
     gene            10095..10316
                     /locus_tag="AC1LW8_000375"
     CDS             10095..10316
                     /locus_tag="AC1LW8_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HIP4772243.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     ADSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            10326..10733
                     /locus_tag="AC1LW8_000376"
     CDS             10326..10733
                     /locus_tag="AC1LW8_000376"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HIP4772244.1"
                     /translation="MEIEIKFNETFEAPMGSPRPRFRNTGRFVQTYMPTAYTNHKAYI
                     QGQMPKLNLERALKIELDFYFPLLKSWSKKKKSEMVGQYKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGLEPKIIMRVEEVI"
     gene            10733..10918
                     /locus_tag="AC1LW8_000377"
     CDS             10733..10918
                     /locus_tag="AC1LW8_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HIP4772245.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQYFDDVDIEKEALADYLYNNPDE
                     LLEYDNLKIRNVNVEVE"
     gene            10919..11278
                     /locus_tag="AC1LW8_000378"
     CDS             10919..11278
                     /locus_tag="AC1LW8_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA1788 family PVL leukocidin-associated protein"
                     /protein_id="HIP4772246.1"
                     /translation="MSVVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LNAPYGMRLAEYKEIVLSRIMQRESKEREIARQRRKEVELRKRKPHLFNVPQKHSRDP
                     YWFDVTYNQMFKKWQEV"
     gene            11279..11527
                     /locus_tag="AC1LW8_000379"
     CDS             11279..11527
                     /locus_tag="AC1LW8_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HIP4772247.1"
                     /translation="MPKTDSARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            11542..11778
                     /locus_tag="AC1LW8_000380"
     CDS             11542..11778
                     /locus_tag="AC1LW8_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HIP4772248.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMAGQVVYKYEEE"
     gene            11783..12316
                     /locus_tag="AC1LW8_000381"
     CDS             11783..12316
                     /locus_tag="AC1LW8_000381"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIP4772249.1"
                     /translation="MTNTLQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            12353..12559
                     /locus_tag="AC1LW8_000382"
     CDS             12353..12559
                     /locus_tag="AC1LW8_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HIP4772250.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            12556..12750
                     /locus_tag="AC1LW8_000383"
     CDS             12556..12750
                     /locus_tag="AC1LW8_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772251.1"
                     /translation="MTDVKIKTISGGVYFVKTAEPFEKYVERTVNFNGFIYVSNIIKQ
                     PTYIKTDTIESITLIEERGK"
     gene            12747..12950
                     /locus_tag="AC1LW8_000384"
     CDS             12747..12950
                     /locus_tag="AC1LW8_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772252.1"
                     /translation="MNQLRILLHDGSSLILHEDELFNEIVFVLDNFRNDDDYLTIEKD
                     YGRELVLNKGYIVGINVEEADDD"
     gene            12943..13179
                     /locus_tag="AC1LW8_000385"
     CDS             12943..13179
                     /locus_tag="AC1LW8_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7366 family protein"
                     /protein_id="HIP4772253.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKTPEEKAKIEDDFIKEINDKDS
                     EFYSPMMANMNEHELRAMLRMMPSLIDTGDDNDD"
     gene            13172..13345
                     /gene="rinB"
                     /locus_tag="AC1LW8_000386"
     CDS             13172..13345
                     /gene="rinB"
                     /locus_tag="AC1LW8_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HIP4772254.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEMPSDFAKL
                     SDQSDLMRAEVSE"
     gene            13346..13492
                     /locus_tag="AC1LW8_000387"
     CDS             13346..13492
                     /locus_tag="AC1LW8_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772255.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFEG"
     gene            13507..13926
                     /locus_tag="AC1LW8_000388"
     CDS             13507..13926
                     /locus_tag="AC1LW8_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP4772256.1"
                     /translation="MSLRKSTQRYLESELSNYNYFDKDIARVRDEVLNPWSQQDTNIG
                     GDRVQSNVSVTEIKAIRVVNDRRLSQLARMKSAIEVVYNHSTTETQKLMELYYFKKPR
                     TLNLTGVAQEINVSKSTAYDMRKDILVRLADELGIIH"
     gene            14113..14553
                     /locus_tag="AC1LW8_000389"
     CDS             14113..14553
                     /locus_tag="AC1LW8_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIP4772257.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAISAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES"
     gene            14540..15817
                     /locus_tag="AC1LW8_000390"
     CDS             14540..15817
                     /locus_tag="AC1LW8_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIP4772258.1"
                     /translation="MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNVSSKVDTIKSLGL"
     gene            15828..17363
                     /locus_tag="AC1LW8_000391"
     CDS             15828..17363
                     /locus_tag="AC1LW8_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIP4772259.1"
                     /translation="MLKANEFETDTDLRENRNYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHLDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIVGVRYLRTKPIDKTDEDEVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENVFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTRSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            17370..18365
                     /locus_tag="AC1LW8_000392"
     CDS             17370..18365
                     /locus_tag="AC1LW8_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HIP4772260.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKDFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPNKKRKTKYFGGKVKSDD"
     gene            18438..18608
                     /locus_tag="AC1LW8_000393"
     CDS             18438..18608
                     /locus_tag="AC1LW8_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772261.1"
                     /translation="MSNTDKYFRDIARELKGIRKELQKRNETVIIDANLDSVRSAVLA
                     NKEKPKYNEPLF"
     gene            18636..18723
                     /locus_tag="AC1LW8_000394"
                     /pseudo
     CDS             18636..18723
                     /locus_tag="AC1LW8_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18717..19337
                     /locus_tag="AC1LW8_000395"
     CDS             18717..19337
                     /locus_tag="AC1LW8_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HIP4772262.1"
                     /translation="MEENKLKFNLQFFADQSNDPDEPGGDGKKGNPDKKEYDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAKEKAEEAAKEAAKLAKMNKDQKDEYERKQL
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            19351..20325
                     /locus_tag="AC1LW8_000396"
     CDS             19351..20325
                     /locus_tag="AC1LW8_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIP4772263.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADKRTDSVPGEV"
     gene            20347..20634
                     /locus_tag="AC1LW8_000397"
     CDS             20347..20634
                     /locus_tag="AC1LW8_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772264.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYNPTDERIKALA
                     SVENKRNIQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            20643..20975
                     /locus_tag="AC1LW8_000398"
     CDS             20643..20975
                     /locus_tag="AC1LW8_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HIP4772265.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            20972..21274
                     /locus_tag="AC1LW8_000399"
     CDS             20972..21274
                     /locus_tag="AC1LW8_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772266.1"
                     /translation="MRYEDRVIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            21274..21621
                     /locus_tag="AC1LW8_000400"
     CDS             21274..21621
                     /locus_tag="AC1LW8_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HIP4772267.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            21633..22016
                     /locus_tag="AC1LW8_000401"
     CDS             21633..22016
                     /locus_tag="AC1LW8_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772268.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     IAQLVDDTTNQELLHTSITISYKTF"
     gene            22035..22616
                     /locus_tag="AC1LW8_000402"
     CDS             22035..22616
                     /locus_tag="AC1LW8_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HIP4772269.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDSSTVTPGEV"
     gene            22678..23043
                     /locus_tag="AC1LW8_000403"
     CDS             22678..23043
                     /locus_tag="AC1LW8_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HIP4772270.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVEAEEKPAEA"
     gene            23073..23417
                     /locus_tag="AC1LW8_000404"
     CDS             23073..23417
                     /locus_tag="AC1LW8_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772271.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            23434..26901
                     /locus_tag="AC1LW8_000405"
     CDS             23434..26901
                     /locus_tag="AC1LW8_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772272.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ANDKKLQKTIKVAKKSLDAFSNKNVKAKLDASIQDLQQKILESNFELDKLNSKEASPE
                     VKLQKQKLTKDIAEAEVKLSELEKKRISIDVNADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSVLI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLSDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEVSQSVFKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKTVASVVWNAILKAITTAVTNVYNFIMIVWNQIAAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQG
                     FVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKK
                     ALDAAKSALGIHSPSREFMDVGVYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            26914..27861
                     /locus_tag="AC1LW8_000406"
     CDS             26914..27861
                     /locus_tag="AC1LW8_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HIP4772273.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVSCFCKVKNRSDIAYMRDMLYRLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFNGVINPKQKDNKVSFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRKYTFENLSKGTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDKIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            27870..29771
                     /locus_tag="AC1LW8_000407"
     CDS             27870..29771
                     /locus_tag="AC1LW8_000407"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIP4772274.1"
                     /translation="MTITIKPPKGNGAPVPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            29786..31696
                     /locus_tag="AC1LW8_000408"
     CDS             29786..31696
                     /locus_tag="AC1LW8_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772275.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDAFTKKVEKAVDENYKEYRATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     GNELWIYSAVLDANENNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENL
                     MIFRREYKASERQLKNSLNFVEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSKPANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESNRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGV
                     GKWSLFEGKVVE"
     gene            31696..33519
                     /locus_tag="AC1LW8_000409"
     CDS             31696..33519
                     /locus_tag="AC1LW8_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HIP4772276.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNKPLSISKHNAMTSIVLKTDNFDDEHGAYISDELTIVDAINGRMQYVIPNEFLK
                     YTGRVHAQAYFTQNGSNNVIVERQFSFNIQNDLISNFDGKTKLVYIKSIQDLTESVKE
                     EVEDLKKSLSDTKSLVTEIDSRINQGIQRLEIKQNEAVQMITTTQDKAVQYINSEFQK
                     IVDKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESSEQSIEAVLR
                     HANSSMIIHITNAKDVPEKADIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNTLNQAKQYVD
                     GKLQSISWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWATPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            33519..33896
                     /locus_tag="AC1LW8_000410"
     CDS             33519..33896
                     /locus_tag="AC1LW8_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HIP4772277.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSASDLSDEELRGMVASMQMQVVQVNVLTMELAQ
                     QNAMLTQQLTELKTNKTSTEGDV"
     gene            33900..34073
                     /locus_tag="AC1LW8_000411"
     CDS             33900..34073
                     /locus_tag="AC1LW8_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HIP4772278.1"
                     /translation="MKMIYPTFKDIKTFYVWGYYKNEQIKWYVDKGLIDKEEYALITG
                     EKYPETKDEKSQV"
BASE COUNT        12775 a         5029 c         6600 g         9717 t
ORIGIN      
        1 tacatacttt ttagaatcat atatgtatga agtaaagtgt ttagacaaat atttgatatc
       61 aatattacga aaattatata tttcttttaa tttactgtca tttgagataa cgacgatgca
      121 aggttcttca aaaaaagatt gatttagata aaatatcgaa atcttgtaat cgtcttttct
      181 catgaatggg aaagcttctg gattgctact aaactgataa ttgtatctgt tttcaactac
      241 atatttgtag ccttctaaaa atttacgcaa gtattctttt aaagttttat tctcttccat
      301 ccctcatcct cctcacgcca cacaagcgct attaatcaat atccaataat tgttgttttt
      361 tcttatcgaa ctcttcctga gaaattactc cgacatctaa taattcttta tattttatta
      421 attcatcagc aacagaaaaa ctcatttttt cagaattgga tggtttcata gaactttctc
      481 gaatagagat ttgttcttgt attgtttccg ccattctaga tacagtgttt tttgatatgc
      541 ttcctatagc gatacttgat gaaccgtgat gtataattat ttcgccaaaa agaagtcctt
      601 ttttatacga aacagaattg attttctcga atggaaattc atgaaatttc aaaccatata
      661 tcataccttt atctaagaat aacaatctta gatcagtaca tactattaag taggtattat
      721 tattgtacaa tcccgaagtt acatacatta tgttttcatt atcttttaaa atcataggta
      781 gttctttcac ttcttttttt gtaccaaaca aatcctctac acctatttcg ctaaatcttt
      841 ggtagatttt agataagttt tcatcagatt tattgatttc actttcaaat ttcacttctt
      901 ttctaggttt actttggtat tcttttaaaa tttctctttt gtcttcaaca gatagttgct
      961 tgtattgttt cttttcttct tttgttttag ttgctaaata ttgactctca atcatacttt
     1021 ctttgaacgt taatctgctc ttaggtaatt ctttcatgtt catttctcct ttattttttg
     1081 attgttaaat cgttagatca taagcatatt taaattcatt tataaaatca gatttgcttt
     1141 ccattttctc ttctaaaaaa cttaagtagt tttctgcgtg gtaattttcg ttatttgaca
     1201 tatagtcgtt taacccattg tgtatatgtc ttctgattac ttttaccgct atatggatcg
     1261 cttgaaaact catttgatac ttgtacgaaa tttgctcaat attaaagttg tttatatatt
     1321 tgtatcttat atgtaaagga aacaataaac atgaagcaaa tgagtttgct tcatattctt
     1381 cagcaatcct tctataataa tctttatatg tgaatgtttt atttaaatta actccagtat
     1441 gtcccattat aaaatgacca tattcatgag ctaaagtaaa tcttagacga ttcataggca
     1501 gtaaatcgtt ataaactata atcgctttgt ctccttttct aatatgaaac gcttcttctg
     1561 aaccgaaaat agaaggtatt ttaaaatata aagtgccagt attctgagaa aattcagaga
     1621 aagtcactaa tttaatacgt ttatcttttg agataatttc aaatatatct aaaggaaaag
     1681 ataagttata tagaccattt gtgatctcgt aaactgcttt cgcagattta aaaaagattt
     1741 ttcataattt aatttcaatt aaaaagcccc tttgttactt agttaaatca tcccaatcat
     1801 caaacattgc ttctaatata gtcaaagctt tttgcctttg tgcctccgtc atattttctg
     1861 tagctcgatg cataataaga atatcttcac ttttatcttc tccggagtac tcatcttttt
     1921 ctctacctaa taagtaatca actgatacat cgaagtgatc ggcaattttt tgcaccttat
     1981 caatgcctgg tttggttttc tcccatcttc tgatttgtcc gtttgaaaac cctaaagttc
     2041 tctctaattc agcaaaagtc ataccttttg aattgcacaa attacggatt ctttgtacta
     2101 gattcataaa tttctcctat cacagattaa ctttttcgct atttttgttg acaattagca
     2161 taaaagttaa tatactgtat ttaagcttta aatttagctt actaaacaca taacaattat
     2221 tcgttgggga acgagtattc aataccttta tgacaggcat tacgaattgt tataggttta
     2281 ttaaactatg cttaaatatt agcataaaag ttattggtgt tcaacagata atttatttgc
     2341 ttagaaaaaa tgttatagga ggtgctaata tgtcgacaac agatttcggc ttgaaagtga
     2401 gaacggaatt attaaaacgc aacatgacaa acaagcaact tgcggaaatg ctagaaattt
     2461 caagtgctta cttatcggat attttacgtg gacgtagaga tgcttttgaa caaaagaaac
     2521 gtattgcgaa aattttagaa attaaagaag aggtgaagag ttaatgaatg aaattaaaac
     2581 tttcagtaac gacatgtttt caatcttaat caaacaagat aatgaaaata atttattcga
     2641 tttagaaact gtcgcaaaaa gtttggggtt cactcagttt aaaaacggca aacaatatat
     2701 tcgttgggaa actatcaata aatatttagg taaatatctt tcccaagaag ttgggaaagg
     2761 cgatttcata ccagaaccaa tggtatataa gttggctttc aaagcaggta atgctgtagc
     2821 agaaaaattt caagattggt tggcgatgga agtcctacca gctattcgca aacacggtat
     2881 ctacgcaaca gacaatgtaa ttgaacaaac attaaaagat ccagactaca tcattacagt
     2941 gttgactgag tataagaaag aaaaagagca aaacttactt ttacaacaag aaatcggaga
     3001 actaaaaccc aaagcagact atgtagatga aatcttaaaa tcaactggca cattagctac
     3061 aactcaaatc gcggcagact acggtatatc agcacaaaag ttaaacaaac tactacacga
     3121 agctagacta caacgaaaag taaataaaca gtgggtgctt tactcagaac acatgggcaa
     3181 gagttacaca gattcagaca ctataacaat tgtgcgttct gatggcagag aagacacagt
     3241 tttacaaact agatggacac aaaaaggcag attgaaaata catgaaatca tgactgaatt
     3301 cggttatgaa gctaatttag ggggagcgta aatgacacca gaacaaaaag aaaagctaaa
     3361 caatatagta ttaacacttt atgcagttaa agaaaacaaa agtcaaacat acacacacaa
     3421 agatactctt actgtgacat atgcaggcga gattgagcac acttacgaag tcgacagaga
     3481 gaaacacctt gaatcaatga ttgagtgggc aattgaccaa atcgaacagc actttgattt
     3541 agacgaagaa gaataacaca caattgaaca aacaacttaa taggaggaac tacaaatgtt
     3601 acaaaaattt agaatcgcta aagaaaaaag taaattaaaa ctcaatttac taaaacatgc
     3661 aaacagtaat ttagaaacaa gaaacaaccc tgaactgttg cgagcagttg cagagttgct
     3721 taaagagatt aatcgataaa ttagagaact aatcatcgtt tcagttctcg tcatctaagt
     3781 aattagcaat tacgtaaagt gttaaaagag ttgctattac attaaaaaac ataaagtagt
     3841 tcacatattg gtcaagtatc gttctaacca taaccggaga tgttactccg atagcagaac
     3901 cgacagaaac cttttttata aattttgcag aaggagaaat aaaaatatga ttgagcgatt
     3961 cattgaatac actattgtat ttcttagtat ttacgtcatc tattggatgg gcagaatcga
     4021 cggttttacc aagaacaggg acatcgacag tatcgacaaa agaatttctc aaatgagtgc
     4081 taactttgcg gacttcatcc tccggaaagt tgttagaact tatgaattca tcaagaattt
     4141 tttcagaaaa taaattagtt ttgaacattg gatgattctt agttactcga tgcatatagg
     4201 aagcccaatc agataattta gattggttaa ttctaatgct attcataaca ttattaaccg
     4261 tcgattgaat ttccagagcg ttcataacat acgaattatt catagtattt gtggctttag
     4321 cgtaagcttc gacaggcaat ttagataaga tagctttatt tttctttatt aaatctaact
     4381 gtcgttgagt gagatttata ttattcataa ttaccacctc ctttcactag gagataacta
     4441 aattatacac gaaaggaatg gtagaagtgc caccacacat tcaacaaatg ttatacgaaa
     4501 tccagttaaa agctggtata cctcaaaaat taatggaaat gcaaggtttg ataaacgatg
     4561 aaacaaccaa agaggagaaa aaagaaaatg agtaacattt ataaaagcta cctagtagca
     4621 gtactatgct tcacagtctt agcaattgtg cttatgccat tgctgtactt cactacagca
     4681 tggtcaattg cgggattcgc aagtatcgca acattcatgt actacaaaga atgctttttc
     4741 aaagaataaa aaaactgcta cttgttggag caagtaacag tatcaaacac ttaagaaaaa
     4801 attcatgttc aatataaaac gaaaaacgga ggaagtcaag atgtattacg aaataggcga
     4861 aatcatacgc aaaaatattc atgttaacgg attcgatttt aagctattca ttttaaaagg
     4921 tcatatgggc atatcaatac aagttaaaga tatgaacaac gtaccaatta aacatgctta
     4981 tgtcgtagat gagaatgact tagatatggc atcagactta tttaaccaag caatagatga
     5041 atggattgaa gagaacacag acgaacagga cagactaatt aacttagtca tgaaatggta
     5101 ggaggtcgct atgaagcaga ctgtaactta tatcattcgt catagggata tgccaattta
     5161 tataactaac aaaccaactg ataacaattc agatattagt tactccacaa atagaaatag
     5221 agctagggag tttaacggta tggaagaagc gagtatcaat atggattatc acaaagcaat
     5281 caagaaaaca gtgacagaaa ctattgagta cgaggaggta gaacatgact gaggaaaaac
     5341 aagaaccaca agaaaaagta agcatactca aaaaactaaa gataaataat atcgctgaga
     5401 aaaataaaag gaaattctat aaatttgcag tatacggaaa aattggctca ggaaaaacca
     5461 cgtttgctac aagagataaa gacgctttcg tcattgacat taacgaaggt ggaacaacgg
     5521 ttactgacga aggatcagac gtagaaatcg agaactatca acactttgtt tatgttgtaa
     5581 attttttacc tcaaatttta caggagatga gagaaaacgg acaagaaatc aatgttgtag
     5641 ttattgaaac tattcaaaaa cttagagata tgacattgaa tgatgtgatg aaaaataagt
     5701 ctaaaaaacc aacgtttaat gattggggag aagttgctga acgaattgtc agtatgtaca
     5761 gattaatagg aaaacttcaa gaagaataca aattccactt tgttattaca ggtcatgaag
     5821 gtatcaacaa agataaagat gatgaaggta gcactatcaa ccctactatc actattgaag
     5881 cgcaagaaca aattaaaaaa gctattactt ctcaaagtga tgtgttagct agggcaatga
     5941 ttgaagaatt tgatgataac ggagaaaaga aagttagata tattctaaac gctgaacctt
     6001 ctaatacgtt tgaaacaaag attagacatt caccttcaat aacaattaac aataagaaat
     6061 ttgcaaatcc tagcattacg gacgtagtag aagcaattag aaatggaaac taaaaattaa
     6121 ttaaaaggac ggtatttaat tatgaaaatc acaggacaag cgcaatttac taaagaaaca
     6181 aatcaagaaa agttttataa cggctcagca gggtttcaag ctggagaatt cacagtgaaa
     6241 gttaaaaata ttgaattcaa tgatagagaa aatagatatt tcacaatcgt atttgaaaat
     6301 gatgaaggca aacaatataa acataatcaa tttgtaccgc cgtataaata tgatttccaa
     6361 gaaaaacaat tgattgaatt agttactcga ttaggtatta agttaaatct tcctagctta
     6421 gattttgata ccaatgatct tattggtaag ttttgtcact tggtattgaa atggaaattc
     6481 aatgaagatg aaggtaagta ttttacggat ttttcattta ttaaacctta caaaaagggc
     6541 gatgatgttg ttaacaaacc tattccgaag acagataagc aaaaagctga agaaaataac
     6601 ggggcacaac aacaaacatc aatgtctcaa caaagcaatc catttgaaag cagtggccaa
     6661 tttggatatg acgaccaaga tttagcgttt taaggtgtgg tttaaatgca atacattaca
     6721 agataccaga aagataacga cggtacttat tccgtcgttg ctactggtgt tgaacttgaa
     6781 caaagtcaca ttgacttact agaaaacgga tatccactaa aagcagaagt agaggttccg
     6841 gacaataaaa aactatctat agaacaacgc aaaaaaatat tcgcaatgtg tagagatata
     6901 gaacttcact ggggcgaacc agtagaatca actagaaaat tattacaaac agaattggaa
     6961 attatgaaag gttatgaaga aatcagtctg cgcgactgtt ctatgaaagt tgcaagggag
     7021 ttaatagaac tgattatagc gtttatgttt catcatcaaa tacctatgag tgtagaaacg
     7081 agtaagttgt taagcgaaga taaagcgtta ttatattggg ctacaatcaa ccgcaactgt
     7141 gtaatatgcg gaaagcctca cgcagacctg gcacattatg aagcagtcgg cagaggcatg
     7201 aacagaaaca aaatgaacca ctatgacaaa catgtattag cgttatgtcg cgaacatcac
     7261 aacgagcaac atgcgattgg cgttaagtcg tttgatgata aataccactt gcatgactcg
     7321 tggataaaag ttgatgagag gctcaataaa atgttgaaag gagagaaaaa ggaatgaata
     7381 gactaagaat aataaaaata gcactcctaa tcgtcatctt ggcggaagag attagaaatg
     7441 ctatgcatgc tgtaaaagtg gagaaaattt taaaatctcc gtttagttaa tacaggtttt
     7501 tacaaaagct ttaccatagg cggacaaact aattgagcct tttttgatgt ctattaccca
     7561 ggggctgtaa tgtaacttta atacttcaaa ttcaatgcca gaaagtttac ttattgtttc
     7621 taggttgtgt cctgacttta acattctttt aacaaattct aatcccgaaa caaatctttg
     7681 tttttctata atcttattaa agtgatttaa aaactgagga gcataaaact tattataaat
     7741 tccttttttt gttaagtaag acatgtcaaa agtttcattt aaaaccccta accttactag
     7801 gttattaatt gaaatttcgg ttgattctat atctaacgga gagtctttta ttaacgtgtc
     7861 cgatatattc ataccgtcat tctttgggtt taaaaccgct ctatatttaa cggcaggatg
     7921 tacttcgtga ttctttaaat gttttaaaag aatagcatca tttggggata attgtttaat
     7981 tatttcaaca aatgaatggt gggttaatga gttttttctg tcatccatag atgatgctat
     8041 tagttttgcg aacatattac ttaaagtttt ttcactaatg taaaactttg aagcttctag
     8101 agcaggacct agaagagaaa attgtggttc ttgtaaatta ttttcaggta cagaagatat
     8161 ttctttttta aattgttctt tgaatttttc aaattctact tctctttgat aaataacttt
     8221 atccacataa aggtggaatt tcccaaagac aagttcccaa gttttagaga atgtttctac
     8281 aggccctttt gatgcgcctt caataatttt atcaatacct ttacctaaaa taggatccat
     8341 aattattcac ccccaatcta acgcagtagc gataacaaaa ttataccaga aaggagaatc
     8401 aacatgactg accaaccaag ttactactca ataattacag caaatgtcag atacgataac
     8461 cgacttactg acagcgaaaa gttacttttt gcagaaataa catctttaag taacaaatac
     8521 ggatactgca cagcaagtaa tggttacttt gcgaaactat atgaagttac aaaagttact
     8581 gtatcacgcc gtatagccaa cttaaaagaa tgtggatatt tacatgttga aatcattaga
     8641 aatggtaatg aaattaaaca aagaaagcta taccccttaa cagaaatgat aagacctatt
     8701 aacacaaatg ataatacccc tattaacaat tctgttaata cccctattat cacaaatgtt
     8761 aaagagaata atacaagtat taataataca agtaataaca atataaatag agtagatata
     8821 ttgtcgggaa acccgacacg catcccatat aaagagatta ttgattatct taacgaaaag
     8881 actggcaaga agtttaaaca taatacaact aaaacgaaag attttattaa agcaaggtgg
     8941 aatcaagatt ttaggttgga ggattttaaa aaggtaattg atattaaaac agctgagtgg
     9001 cttaacacgg atagcgataa ataccttaga ccagaaacac tttttggcaa taaatttgag
     9061 ggatacctca atcaaaaagt acaaccaact ggcacggatc aattagaacg catgaagtac
     9121 gacgaaagtt attgggatta gagggatatt atgaaaccac tattcagcga aaagataaac
     9181 gaaagcttaa aaaaatatca acctactcat gtcgaaaaag gattgaaatg tgagagatgt
     9241 ggaagtgaat acgacttata taagttcgct cctactaaaa aacacccgaa tggttatgag
     9301 tataaagacg gttgcaaatg tgaaatctat gaggaatata agcgaaacaa gcaacggaag
     9361 ataaacaaca tattcaatca atcaaatgtt aatccgtctt taagagatgc aacagtcaac
     9421 aactacaaac cacaaaatga aaaacaagta cacgctaaac aaacagcaat agagtatgta
     9481 caaggtttct ctacaaaaga accgaaatca ttaatattgc aaggttcata cggaactggt
     9541 aaaagccacc tagcatacgc tatcgcaaaa gcagttaaag ctaaaggaca tacggttgct
     9601 tttatgcaca taccaatgtt gatggatcgt atcaaagcga catacaacaa aaatgcagta
     9661 gagactacag acgagctagt cagattgcta agtgatattg atttacttgt actagatgat
     9721 atgggtgtag aaaacacaga gcacacttta aataaacttt tcagcattgt tgataacaga
     9781 gtaggtaaaa acaacatctt tacaactaac tttagtgata aagaactaaa tcaaaatatg
     9841 aactggcaac gtatcaattc aagaatgaaa cacaatgcga gaaaagtaag agtaatcgga
     9901 gacgatttca gggagcgaga tgcatggtaa ccaaagaatt tttaaaaact aaacttgagt
     9961 gttcagatat gtacgctcag aaactcatag atgaggcaca gggcgatgaa aataggttgt
    10021 acgacctatt tatccaaaaa cttgcagaac gtcatacacg ccccgctatc gtcgaatatt
    10081 aaggagtgtt aaaaatgccg aaagaaaaat attacttata ccgagaagat ggcacggaag
    10141 atattaaggt catcaagtat aaagacaacg taaatgaagt ttattcgctc acaggagccc
    10201 atttcagcga cgaaaagaaa atcatggctg atagtgacct aaaacgattc aaaggcgctc
    10261 acgggctttt atatgagcaa gagctagggt tacaagcaac gatatttgat atttagaggt
    10321 ggcacatgga aatagaaatt aaatttaacg aaacgttcga ggcacctatg ggctcgcctc
    10381 gtccacgctt tcgtaataca ggtagatttg ttcaaacata catgccaaca gcttatacaa
    10441 atcataaagc gtatatacaa gggcaaatgc ctaagttaaa tctagagcgc gcactaaaaa
    10501 tcgaattaga cttttacttt ccattactta aatcatggtc gaagaaaaag aaaagtgaaa
    10561 tggttggaca gtataaagtg actaagccgg atatcgataa cttaattaaa acagtattag
    10621 acgcatgtaa tggtcatgtg tggaaagacg ataaccaaat tacagaaata actagctcaa
    10681 agcgttatgg actagaacca aaaataatca tgcgagttga ggaagtgatc taatgcaaca
    10741 gcaagcatat ataaacgcaa cgattgatat aaggatacct acagaagttg aatatcagta
    10801 ttttgatgat gtggatatcg aaaaagaagc gctggcagat tacttatata acaatccaga
    10861 cgaattacta gagtatgaca atttaaaaat tagaaatgta aatgtagagg tggaataaat
    10921 gagtgtcgtg aagattaacg gtaaaccata taaatttacc gaacatgaaa atgaattgat
    10981 aaaaaagaac gggttaactc ctggaatggt tgcaaaaaga gtacgtggtg gctgggcgtt
    11041 gttagaagcc ttaaacgcac cttatggcat gcgcttagct gagtataaag aaatcgtatt
    11101 atccagaatt atgcaacgag agagcaaaga acgtgaaata gctaggcaac gacgtaaaga
    11161 ggttgaacta cgtaagagga aaccacattt gtttaatgta ccacaaaaac attcacgtga
    11221 tccgtactgg ttcgatgtca cttataacca aatgttcaag aaatggcagg aagtataaat
    11281 gcctaaaacc gatagcgcac gcaaagaata cttaaaccaa tttttcggat ctaagagata
    11341 tctgtatcag gataacgaac gagtggcaca tatccatgta gtgaatggca cttattactt
    11401 tcacggtcat atcgtgccag gttggcaaag cgttaaaaag acatttgata ctgctgaaga
    11461 gctcgaaata tatataaagc aacatggttt ggaatacgag gaacagaagc aactaacttt
    11521 attttagagg agatggaaat gatgaataat cgtgaacaaa ttgaacaatc cgttataagt
    11581 gctagtgcgt ataacggcaa tgacacagag gggttgctaa aagagattga ggacgtgtat
    11641 aagaaagcgc aagcgtttga tgaaatactt gagggaatga caaatgctat tcaacattca
    11701 gttaaagaag gtattgaact tgatgaagca gtagggatta tggcaggtca agttgtctat
    11761 aaatatgagg aggaatagga aaatgactaa cacattacaa gtaaagctat tatcagaaaa
    11821 cgctagaatg ccagaacgaa atcataagac ggatgcaggt tatgacatat tctcagctga
    11881 aactgtcgta cttgagccac aagaaaaggc agtgatcaaa acagatgtag ctgtaagcat
    11941 accagagggc tatgtcggac tattaactag ccgtagtggt gtaagtagta aaacgcattt
    12001 agtgattgaa acaggcaaga tagacgcggg atatcacggc aatttaggga ttaatatcaa
    12061 gaatgataat gaaacgttag agagtgagga tatgagtaac tttggtcgga gtcctgctgg
    12121 gatagatgga aagtatgccc gactacctgt aacagataaa attttatgta tgaatggtag
    12181 ttatgtcata aacaaaggcg acaaactagc tcaattggtt attgtgccta tatggacacc
    12241 tgaactaaag caagtggagg aattcgagag tgtttcagaa cgtggagcaa aaggcttcgg
    12301 aagtagcgga gtgtaaagac atcttagatc gagtcaagga ggttttgggg aagtgacgca
    12361 atacttagtc acaacattca aagattcaac aggacgcaag catacacaca taactcgagc
    12421 taaaagcaat caaaggttta cagttgttga ggcagagagt aaagaagaag cgaaagagaa
    12481 gtacgaggcg caagttaaaa gagatgcagt tattaaagtg ggtcagttgt ttgaaaatat
    12541 aagggagtgt gggaaatgac ggatgttaaa attaaaacta tttcaggtgg agtttatttt
    12601 gtaaaaacag ctgaaccttt tgaaaaatat gttgaaagaa cggtaaattt taatggtttt
    12661 atttacgtaa gtaatataat caaacagcca acgtatatta aaacagatac gattgaatca
    12721 atcacactta ttgaggagcg tgggaaatga atcagctgag aattttatta catgacggta
    12781 gtagtttgat attacatgaa gatgaattat ttaacgaaat agtatttgtt ttggacaatt
    12841 ttagaaatga tgatgactat ttaacgatag aaaaagatta tggcagagaa cttgtattga
    12901 acaaaggtta tatagttggg atcaatgttg aggaggcaga cgatgattaa tattcctaaa
    12961 atgaaattcc cgaaaaagta cactgaaata atcaaaaaat ataaaaataa aacacctgaa
    13021 gaaaaagcta agattgaaga tgatttcatt aaagaaatta atgataaaga cagtgaattt
    13081 tacagtccta tgatggctaa tatgaatgaa catgaattaa gggctatgtt aagaatgatg
    13141 cctagtttaa ttgatactgg agatgacaat gatgattaaa caaatactaa gactattatt
    13201 cttactagca atgtatgagt taggtaagta tgtaacggag caagtatata ttatgatgac
    13261 ggctaatgat gatgtagaga tgccgagtga cttcgcaaag ttgagcgatc agtctgattt
    13321 aatgagggcg gaggtgtcag agtagatgat gtggttagtc atagcaatta tattactagt
    13381 catcttattg tttggtgtga tgttgcaagc tgaacagtta aaaggcgatg tgaaagttaa
    13441 agagcgggag atagagatat taagaagtag attgagacat tttgaaggtt aacgggggtt
    13501 aaacaaatga gtttgagaaa atcaacgcaa agatatttag aaagtgaatt aagcaattac
    13561 aattacttcg ataaagatat agcgcgtgta agagatgaag ttttaaaccc gtggagtcaa
    13621 caagatacta atatcggtgg agatagggtg caaagcaatg taagtgtaac tgaaataaaa
    13681 gctattagag ttgttaatga tagaagatta tcgcaattgg ccagaatgaa atcggctata
    13741 gaggttgtat ataatcatag cactacagaa actcaaaaac ttatggaact ttattatttt
    13801 aaaaagccta gaacattaaa tttaactggt gtagctcaag aaataaatgt aagtaaatct
    13861 accgcttatg atatgaggaa agatatatta gttaggttag ctgatgaatt aggtataata
    13921 cattaagttt ggaaaaagtc tggaaaaata acgtcacttt cggtgttaat atgatagcgt
    13981 aagatattga ctatcttact gcgtttccct tatcgcaatt aggaataaag gatctatgtg
    14041 ggttggctga ttatagccaa tccctttttt aattttaaaa agcgtatagc gcgagagttg
    14101 gtggtaaatg aaatgaacga aaaacaaaag agattcgcag atgaatatat aatgaatgga
    14161 tgtaatggta aaaaagcagc aatttcagca ggttatagta agaaaacagc agagtcttta
    14221 gcaagtcgat tgttaagaaa tgttaatgtt tcggaatata ttaaagaacg attagaacag
    14281 atacaagaag agcgtttaat gagtattaca gaagctttag cgttatctgc ttctattgct
    14341 agaggagaac ctcaagaggc ttacagtaag aaatatgacc atttaaacga tgaagtggaa
    14401 aaagaggtta cttacacaat cacaccaact tttgaagagc gtcagagatc tattgaccac
    14461 atactaaaag tacatggtgc gtatatcgat aaaaaagaaa ttactcagaa gaatattgag
    14521 attaatattg gtgagtacga tgacgaaagt taaattaaac tttaacaaac catctaatgt
    14581 tttcaataga aacatattcg aaatactaac caattacgat aacttcactg aagtacatta
    14641 cggtggaggt tcgagcggta agtctcacgg cgttatacaa aaagttgtac tcaaagcatt
    14701 gcaagactgg aaatatccta ggcgtatact atggcttaga aaagtccaat caacaattaa
    14761 agatagttta ttcgaagatg tcaaagattg tttgataaac ttcggtattt gggacatgtg
    14821 cctttggaat aagactgata acaaagttga attgccaaac ggcgcagttt ttttgtttaa
    14881 aggattggat aatccggaga aaataaagtc gataaaaggc atatcagaca tagtaatgga
    14941 agaggcttct gaattcacac taaatgatta cacgcaatta acgttgcgtt tgagggagcg
    15001 taaacacgtg aataagcaaa tatttttgat gtttaaccca gtatctaaac tgaattgggt
    15061 ttataagtat ttctttgaac atggtgaacc aatggaaaat gtcatgatta gacaatctag
    15121 ttatcgagat aataagtttc ttgatgaaat gacacgacaa aacttagagt tgttagcaaa
    15181 tcgtaatcca gcatattaca aaatttatgc gttaggtgaa tttgctacat tagacaaatt
    15241 ggttttccct aagtatgaaa aacgtttaat aaataaagat gagttaagac atttaccttc
    15301 ttattttgga ttggactttg gctacgttaa tgatcctagt gcttttatac attctaaaat
    15361 agatgtaaag aaaaagaaat tatacatcat tgaagagtat gttaaacaag gtatgctgaa
    15421 tgatgaaata gctaatgtca taaagcaact tggttatgct aaagaagaaa ttacagcaga
    15481 tagtgcagaa caaaaaagta tagctgaatt aaggaatcta ggacttaaaa ggattttacc
    15541 aaccaaaaaa gggaagggct cggttgtgca agggttacaa ttcttaatgc aatttgaaat
    15601 cattgttgat gaacgttgtt tcaagactat tgaagagttt gacaactaca catggcaaaa
    15661 ggacaaagat acaggtgaat ataccaatga accagtagat acatacaatc attgtatcga
    15721 ttcgttgcgt tattcagtgg aacgattcta cagaccggtt agaaaacgca caaatgtcag
    15781 ttcgaaagtt gacacaataa aatctctagg attataggag ggaacaaatg ttaaaggcaa
    15841 acgaatttga aacggatact gatttacgag aaaacagaaa ttacttgttt aacgatgaag
    15901 ctaatgttgt ttacacatat gacgggacgg aatccgattt attacaaaac gttaatgaag
    15961 taagtaaata cattgaacat cacttggatt accaacgacc tagattaaaa gtgttgagtg
    16021 attactacga aggtaaaact aagaatctgg ttgagttaac acgacgcaaa gaagagtaca
    16081 tggcagataa ccgtgtagcg catgattacg catcttatat tagcgatttt attaacggtt
    16141 atttcttagg taatccaatt caatatcaag atgatgacaa agatgtatta gaagctattg
    16201 aggcgttcaa tgatttgaat gatgttgagt cgcacaatag atctttagga ttagatttgt
    16261 caatttatgg taaagcttat gaattaatga ttagaaacca agatgatgaa acgcgtttat
    16321 acaagagtga tgcaatgagt acttttgtca tatacgacaa tacaattgaa cgtaatagta
    16381 tcgtaggcgt tagatattta agaactaaac caatagacaa gactgacgaa gatgaagtgt
    16441 ttacagttga tttatttact tctcacggcg tttatagata tcttaccagt agaacaaatg
    16501 gattgaagct cacaccgcgt gaaaacgttt ttgaatcaca ctctttcgaa cgtatgccta
    16561 ttacagaatt tagcaacaac gaaagaagaa aaggggatta tgagaaagta atcactttaa
    16621 ttgatttgta tgataatgct gaatcagata ctgctaacta tatgagtgat ttaaatgacg
    16681 ctatgttact tattaaaggt aatttaaatt tagatcctgt agaagttaga aaacaaaagg
    16741 aagctaacgt gttgttttta gaaccgactg tttatgctga tagcgaaggt agagaaacag
    16801 aaggttcagt tgacggtggt tatatttata agcaatacga tgtacaaggt accgaagctt
    16861 ataaagaccg tttgaacagt gatatacaca tgtttaccaa cacgcctaac atgaaagatg
    16921 ataactttag tggcactcaa tcgggcgagg caatgaaata caaattattt ggattggaac
    16981 aacgtactaa aactaaagaa ggattgttta ctaaagggtt aagacgtcgt gctaagttgt
    17041 tagagacaat acttaaaaat acacggtcga ttgacgctaa caaagatttc aatactgtta
    17101 gatacgtata caacagaaac ttacctaaat cattgattga agaattaaaa gcttatattg
    17161 attctggcgg gaagattagc caaacaactt taatgtctct attctcgttc ttccaagacc
    17221 ctgaattaga agtcaagaaa atagaagaag atgagaaaga atctattaaa aaagctcaaa
    17281 aaggcattta taaagaccct agagacatca atgatgacga acaagatgat gatacaaaag
    17341 atactgttga taaaaaggaa tgattgtaat tgcctaacaa aaacactcaa gaatattggg
    17401 aagaacgcgg acgcaaagca atcgagaatg agttgaagcg tgataaaact aaagctgaag
    17461 aaatagaacg tatattgaat atgatgatta agcgcattga aaaagagatc aatgcgttta
    17521 ttgttaagta cggagatttt gcaggcgtta cattacaaga agcacaaaag attattgatg
    17581 agttcgatgt aaaagcgttt caagaagaag caaaaagatt ggtcgaaaac aaggacttta
    17641 gcgatagagc aaatgaagaa ttaaagaagt ataacactaa gatgtatgta tctagagaac
    17701 agatgttaaa gattcaaata gaattcttaa ttgcttatgc aacagctcaa acagaattat
    17761 cgatgaggga atatttcgaa tcaacagctt atcgtgtgtt cagtgatcaa gcgggtattt
    17821 taggtgaagg tgtacaagta gctaaagaag ttatagatac aatcgttgat acacaatttc
    17881 atggtgtcgt ttggtcagag cgattatgga ctaataccga agcaatgaaa caagaagtag
    17941 aagaaataat tgctaatgta gttattagag gtcgacatcc taatgaatat gttaaagata
    18001 tgcgcaagca cttaaataaa ttcgaaggca cagcacgaca aaagaccgca gcaattaaat
    18061 cattgcttta tacggaatcg gcacgtgttc acgcacaatc aagcattgac agcatgaaag
    18121 aaatttcacc ggaaggatat tatatgtata ttgcaaaaat cgataataga acaactaaag
    18181 tatgcaaggg gcttaatgga gaaatattca aagttaaaga cgctaaaatt ggtgttaatt
    18241 tctaccctat gcatatcaat tgtcgttcag attgtgcatt actacctaaa tctatgtggc
    18301 cgaaaaaacc aaacaagaaa cgaaaaacaa aatacttcgg aggaaaagtg aaaagcgatg
    18361 attgatttaa aagtaaaagt ttttaaaggc aagttagcat tgtatgatag taaattaagt
    18421 gtttggagga tattggtatg agcaatactg acaaatactt tagagacata gcaagagagt
    18481 taaaaggtat acgtaaagag ttacaaaagc gaaacgaaac agttattatt gatgcaaact
    18541 tagacagcgt aaggtcggca gtattagcca ataaagaaaa accgaaatat aacgaaccac
    18601 tcttttaata gctagcactt aattgtgttg gctatttttt atgtccaaaa cgtgctgatg
    18661 acataaaaag cacgcatgga aaaacagtcg acagactata aatggaggta tatctcatgg
    18721 aagaaaataa acttaagttt aatttgcaat tttttgcaga ccaatcaaat gatccggacg
    18781 aaccaggcgg agatggtaaa aaaggaaatc ctgataagaa agaatatgac gaaggtactg
    18841 aaataacttt cacgccagag caacaaaaga aagttgatga aatacttgaa cgtcgtgtag
    18901 cccacgaaaa gaaaaaagct gatgagtatg caaaagaaaa agcagaagaa gccgctaaag
    18961 aagctgctaa attagcgaaa atgaacaagg atcaaaaaga tgaatatgaa cgcaaacaat
    19021 tggaaaaaga gctggagcaa ttacgctcag aaaaacaatt aaatgaaatg cgttcagaag
    19081 caaggaaaat gttaagcgaa gcagaagttg attcatcaga cgaggttgtt aatttagttg
    19141 taacagatac tgctgaacaa actaaattga atgttgaagc tttttctaat gcagtaaaaa
    19201 aagcggttaa tgaagcggtt aaggttaacg ctagacaatc gccattgact ggtggagatt
    19261 catttaatca ctcgactaaa aataaaccgc aaaacttagc tgaaatagct agacaaaaaa
    19321 gaattattaa aaattaacgg aggcatttaa atggaacaaa cacaaaaatt aaaattaaat
    19381 ttgcaacatt ttgcgagtaa caatgttaaa ccacaagtat ttaaccctga caatgtaatg
    19441 atgcatgaaa agaaagatgg cacgttgtta aacgacttta caacacctat cttacaagag
    19501 gttatggaaa actctaaaat catgcaatta ggtaagtacg aaccaatgga aggtactgag
    19561 aagaagttta ctttttgggc tgataaacca ggtgcttact gggtaggtga aggtcaaaaa
    19621 atcgaaacgt ctaaggctac ttgggttaat gctacaatga gagcgtttaa attaggggtt
    19681 atcttaccag taacaaaaga attcttgaat tacacttatt cacaattctt tgaagaaatg
    19741 aaacctatga ttgctgaagc tttctataaa aagtttgacg aggcgggtat tttgaatcaa
    19801 ggtaacaatc cattcggtaa atcaattgca caatcaattg aaaaaactaa taaggttatt
    19861 aaaggtgact tcacacaaga taacattatt gatttagagg cattacttga agatgacgaa
    19921 ttagaagcaa atgcgtttat ctcaaaaaca caaaacagaa gcttgttacg taaaattgta
    19981 gatcctgaaa cgaaagaacg tatttatgac cgtaacagtg attcgttaga cggtctacct
    20041 gtggttaacc ttaaatcaag caacttaaaa cgtggtgaat taatcactgg tgacttcgac
    20101 aaattgattt atggtatccc tcaattaatc gaatacaaaa tcgatgaaac tgcacaatta
    20161 tctacagtta aaaacgaaga tggcacacct gtaaacttgt ttgaacaaga catggtggca
    20221 ttacgtgcaa ctatgcatgt agcattgcat attgctgatg ataaagcgtt tgctaagtta
    20281 gttcctgctg acaaaagaac agattcagtt ccaggagaag tttaataaat aattaggagt
    20341 ggtaacatgc ccgaaatcat tggaattgtt aaagtagatt ttacagattt agaagataac
    20401 agacatgtct atatgaaagg gcatgtctac cctcgtaaag gttataatcc tacagatgaa
    20461 cgtatcaaag ctttagctag tgttgaaaat aaacgcaaca tacaaatgat ttacattgta
    20521 aatgacaaat taaccaaaaa agaacttgtc gaaatagcaa gtgttgctgg cttacaagtt
    20581 gatgaaaaac aaacaaaagc tgaaattatc aatgcttttg agtcactaga gtaggtggtt
    20641 atatgactac gctagctgat gtaaaaaaac gtattggtct taaagatgaa aagcaagatg
    20701 aacaattaga agaaatcata aaaagttgtg aaagccagtt gttatcaatg ttacctattg
    20761 aagttgaaca aataccggaa aggtttagtt acatgattaa agaagttgca gttaaacgct
    20821 acaacaggat tggtgctgaa ggtatgacat cagaagcggt tgacggacgt agcaatgcgt
    20881 atgaattgaa cgatttcaag gagtatgaag ctattattga taattacttt aatgctagaa
    20941 cgagaactaa aaaaggaagg gctgtgttct tttgagatat gaagatagag ttatttttca
    21001 attagaacaa gtagcaactt acaatcctaa aactagcaaa aaagaaaaca cactaatcac
    21061 ttatgatgcg ataccatgca atattaaccc catttctaga gcaagaaagc aacttgaatt
    21121 tggtgatgta aaaaacgatg taagtgttct gaggataaaa gaatcaatat cttaccctgt
    21181 tagccacgtg ttggttaatg gcattcgcta caagatagtt gatacaagga tatacagaca
    21241 cgaaacgtca tattatatcg aagaggtcaa ttgatgaata tagatggatt agacgcactg
    21301 ttaaaccaat ttcacgatat gaaaaccaac attgatgatg atgtagatga tattttacag
    21361 gaaaacgcca aagaatatgt agtacgagct aaattgaaag ctagagaagt aatgaataag
    21421 ggttattgga ctggtaattt atcacgcaat atcagatata aaaaaactgg cgatttgcaa
    21481 tacactatca catcgcatgc agcttatagt ggtttcttag agtttggtac tcgatacatg
    21541 gaggcagaac cttttatgtg gccagtatat gaggtaataa gaaaatcaac tgtagaagaa
    21601 ttgaaagcgt tgtttgaata ggagataaaa gcatgacacc gaacttacaa ctttataata
    21661 aagcgtatga aacgctacaa ggatatggat tccctgttat ttctcgtaaa gagatgcaac
    21721 aagagattcc gtatcctttt tttgtaataa aaatgccgga gtcaaataga agtaagtaca
    21781 cgtttgatag ttattctggc gatacgaatt tagttattga tatttggagt gtaagcgatg
    21841 atttaggaca tcatgacgga cttgttaaaa ggtgtatcga tgatttaaca cctagcgtta
    21901 aaacaaacga ttatgacttt gaagaagatg atactaacat cgcacagtta gtcgatgata
    21961 ctactaatca agaattgcta cacacatcaa taacgatatc ttacaaaaca ttttaaaaaa
    22021 cggaggaata ttgaatggcg aatatgaaaa atagtaatga ccgtattatt ttgtttagaa
    22081 aagctggcga aaaagtagat gctactaaaa tgcttttttt aactgaatac ggcttatcac
    22141 atgaagctga tacagataca gaggatacga tggatgggtc ttataacact ggtggttcag
    22201 ttgaatcaac aatgtctggt actgctaaaa tgttttatgg tgacgatttt gcagatgaaa
    22261 ttgaagatgc agttgtagat cgcgtattgt atgaggcttg ggaagttgaa agtagaatac
    22321 caggcaaaaa tggagatgct actaaattta aagcgaaata tttccaaggt ttccacaata
    22381 aatttgaatt aaaagcagaa gctaacggta ttgatgaata tgaatatgaa tatggagtga
    22441 atggtcgttt ccaacgtgga tttgcaacgc tacctgaggc tgtaacaaag aaacttaagg
    22501 cgactggata cagattccat gacactacaa aagcagatgc gttaactggc gaagatttaa
    22561 cagcaattcc acaacctaag gtagattcat caacggttac accaggagag gtataaaaat
    22621 agggcgttaa gccctattta ttttgtttaa attaatcatg aatggagatt ttaagttatg
    22681 aatgtagaaa ttaacggaaa gtcattagaa ttaagttttg gttttaaatt tttaagagaa
    22741 atcgataacc gattaggttt aaaagttgag caagcttcta tcggtcaagg tgtatcaatg
    22801 ttgcctgtag gtttagagag tggaaatcct gttgtgattg gcgaagtttt aattgcagct
    22861 acatctcact tgaaaaaaca agcaattact attaataaca ttgatgaagc gctagatgaa
    22921 atcgcagaaa atatcggact agaagaattc ggttcagata ttttaacgga gttgggaaag
    22981 cgacctatga cccgaaacct agtcgaagta gtggaagcgg aagagaaacc agcggaagcg
    23041 taataactta cgacagaatc gttataactt gtatgtcaac acttggtatt acagatttga
    23101 acgttattga gcaaatgaca ttaacagaat ataactatcg aatgtatgcg aaagagtatg
    23161 aaatgctaac ccaagaattc gaacgttaca aacttgcgtt tgctattcgt gatgctgcag
    23221 ctactaaaaa tgttgggaca gaaaataaac ctaaagagga atatgttttt aacaacgcaa
    23281 acgacgtatt gccttatgaa gaaaatatcc aacggcttaa cgaaggtaaa gatataagat
    23341 ttagtagcga acgtgatgaa tacgaaccac aaaataatga attctttaaa gttatagcag
    23401 aatttaataa gcaatagaaa gagaggtgtt aatgtgacgg aatataaaat taaagcgact
    23461 attgaagcta gtgtagccaa attcaaaagg caaattgata gtgcggttaa gtctgtgcaa
    23521 agatttaaac gagtagcaga tcaaactaaa gatgttgaat taaacgctaa cgataaaaaa
    23581 ttacaaaaaa ctatcaaggt tgctaaaaag tctttagatg cctttagcaa caaaaatgta
    23641 aaagctaaat tagatgctag tatacaagac ttacaacaaa agatattaga atcaaatttt
    23701 gaactagaca aacttaactc caaagaagct agccctgagg ttaaactaca aaaacaaaag
    23761 ttaactaaag atatcgctga agcagaagtt aagttatccg aactagaaaa gaagcgtatc
    23821 agtattgacg tcaatgcaga taacagtaaa ttcaatcgag tgttaaaagt atctaaagct
    23881 agtcttgaag cattaaatag gtctaaagcc aaagctatta tagacgtgga caatggtgtt
    23941 gctaactcta aaataaaacg cactaaagaa gagcttaaaa gtattccaaa caaaactaga
    24001 tctcgactag atgtagatac agggctttct ataccaacta tttatgcgtt taaaaaatca
    24061 ttagacgcat tgccgaacaa aaaaacaaca aaggtagatg tcgatactaa tggtttaaag
    24121 aaagcttatg cctacataat aaaagcaaac gacaatttcc aaagacagat ggggaattta
    24181 gctaatatgt tccgtgtgtt cggtactgta ggttctaata tggttggtgg attactaact
    24241 tcatctttta gtgtcttaat acctgtaata gcgagcgtag tacctgtagt atttgcgcta
    24301 ttaaacgcta tcaaagtgtt aactggcggt gtacttgctt taggtggtgc ggtagcaata
    24361 gccggcgctg gctttgtagc atttggcgca atggctatca gcgctataaa gatgcttagt
    24421 gacggcactt tacaagctag ctcagcaaca aacgaataca aaaaagcttt agatggcgta
    24481 aagtcagcat ggactgatat tataaagcaa aatcaatccg ctatattcac aactcttgca
    24541 aacggtttaa atactgttaa aacagcaatg cagagcttac aacctttttt tagtggtatt
    24601 tcaagaggaa tggaagaggt gtctcaaagt gtgtttaaat gggctcaaaa tagcggtgta
    24661 gcatcaaggt tcttcaacat gatgaataca actggtgttt cggtatttaa caagctatta
    24721 agtgctgcag gcggtttcgg tgatggatta gtcaatgtat tcacacaatt agcaccactg
    24781 tttcaatggt cggctgattg gttggataga ttaggtcaat ctttctctaa ctgggctaat
    24841 agtgcagctg gagaaaattc gataactcgt tttattgaat acacaaaaac aaacttacct
    24901 atcattggta atatttttaa aaatgttttc gttggaatta acaatttgat gaatgcattc
    24961 agtggatcat caactggcat tttccaatct cttgaacaaa tgacagctaa gtttagggaa
    25021 tggtctgaac aagtaggaca atctcaaggg tttaaagact ttgtcagtta tatacaaact
    25081 aatggaccac taataatgca attaattggg aacattgcaa gaggattagt tgcattcgca
    25141 acagcgatgg ctcctatagc tagtgcagta ttacgcgttg cagtagcaat aactggttgg
    25201 atagctaact tgtttgaggc gcatccagct acagcacaat tagttggtgt cattataact
    25261 ttagttggtg catttagatt tttaataccg attattcttg ctgtatctaa ctttatgggt
    25321 ggcggattaa taggtagaat cattgcgtta gtaagtaagt tcggtttatt aagagcggga
    25381 ttaacaattt taaaaggtgc gttcatgtta ttaaaaggac cattaaaaat tatatcagtt
    25441 atattccaat tgttattcgg taagattgga ttaattagaa atgctatcac aggactagta
    25501 actgtgtttg gtattttagg tggtccaata acaatagtta tcggtgtaat cgctgcatta
    25561 atagctatat tcgttttatt gtggaataaa aatgaaggat tcagaaactt tattataaat
    25621 gcttggaatg cgataaaaac gtttatggtt acagtttgga atgtgttgaa aactgtagct
    25681 tcggttgtat ggaatgctat tttaaaagct atcactacag cagtaactaa tgtatacaat
    25741 tttataatga ttgtttggaa tcaaatagcc gcttatttac aagggttatg gaatggaatt
    25801 atcgctattg caacaacggt atggaacctt ttagttacaa tcatcacaac tgttttcacg
    25861 acgataatga caatagttat gacgatatgg acagctattt ggacattctt aagtacaatc
    25921 tggaacacga taattacaat cgctactacg atttggaatt tgttagtcac tgtaataact
    25981 acagtattta ccacaattat gactatcgca atgacaattt ggaacgctat ttggacgttc
    26041 ttacaaacgt tgtggaacac tatagttact gtggcaacta aggtttggaa cgctatcact
    26101 acaactatat ctactgcgtt acaagcggca tggagtttta tttctaatat atggaatacg
    26161 atttggagtt tcttatctgg tatattaacg acaatttgga ataaagttgt aagcatattc
    26221 acacaagttg tatcaactat atcagacaaa atgtctcaag cttggaactt catcgtgact
    26281 aaaggtatgc aatgggtatc tactataaca agtacgctaa ttaactttgt taatagagtt
    26341 attcaaggat tcgttaatgt tgtaaacaaa gttagtcaag gtatgacaaa tgcagtaaat
    26401 aaaataaaaa gctttatagg agattttgtg tctgcaggtg ctgatatgat ccgtggttta
    26461 attagaggta ttggacaaat ggctggccaa ttagtagatg cggctaaaaa tgttgctaag
    26521 aaagctttag atgcagctaa aagtgctttg ggtattcact caccttcacg tgaattcatg
    26581 gatgttggtg tgtattcaat gctaggtttc gttaaaggta tagataatca ttcaagtaaa
    26641 gttatccgta atgtttctaa tgttgcagat aaagtagttg atgcatttca acctacatta
    26701 aacgcacctg acatttctag tattacagga aacttaagta atttaggtgg aaatataaat
    26761 gcgcaagtac aacacacaca ttctattgaa acatcaccga acatgaaaac tgttaaagtt
    26821 gaattcgatg tcaataacga tgcgcttact agtattgtta acggcagaaa tgctaaacgc
    26881 aattctgagt attacttata aaggaggtta caaatggaca tagaattaac aaaaaaagat
    26941 ggtactgtaa tcaaattaag tgaatacggg tttatcgtta acgatatagt aattgatagc
    27001 atgcaaatca acacaaagta tcaagacaaa gaaaatatga acggtcgtat attaatgggg
    27061 agcaattata tcagtagaga tatagttgtt tcttgttttt gtaaagttaa aaatcgttca
    27121 gacattgctt atatgcgaga tatgttgtat aggttaacga cagacataga acctatgtat
    27181 ttacgagaaa taagaagaaa agaagagttg aattacaggt ttactcaacc aacttctgat
    27241 gattacgtga aattagataa aaacaacttc ccggattatg aatattcaag acacgatcaa
    27301 caaatttatg taaatggtaa acagtataaa gttattttta acggagttat aaaccctaaa
    27361 caaaaagata ataaagtttc ttttgaacta aaattcgaaa ctacagaatt accatacggc
    27421 gaaagtattg gaacaagcct agagttagaa gaaaacaaaa aggttggatt gtggtcgttt
    27481 gattttaata ttgattggca tgcaggcgga gacaaaagaa agtatacatt tgaaaatttg
    27541 agcaaaggta cagtttacta tcatggtagt gctcctaacg accaattcaa catgtataaa
    27601 aagataacaa ttattttagg cgaagataca gaatcgtttg tatggaattt aacgcatgct
    27661 gaaataatga aaattgaggg gattaaacta aaagctggag acaaaattgt ttatgatagc
    27721 tttcgagttt ataaaaacgg tgtcgaaata agcactgaaa cgaacatagc ccaaccaaaa
    27781 tttaaatacg gagctaataa atttgagttt aatcaaacag ttcaaaaagt tcagtttgat
    27841 ttgaaatttt attataagta ggtgtcagaa tgacaataac tattaaacca cctaaaggta
    27901 atggcgcacc tgtaccagta gaaacaactt tagtaaaaaa agttaatgct gacggtgtat
    27961 taacttttga tattctagaa aataaatata cttatgaagt tattaacgct atagggaaaa
    28021 gatggattgt tagtcatgtc gaaggtgaaa acgacaagaa agaatatgta ataactgtca
    28081 ttgataggaa atcagaaggc gacagacaac tggttgaatg tactgctaga gagattccta
    28141 tagacaagtt aatgattgat aggatttatg ttaatgtaac aggatctttt acagtagaaa
    28201 gatattttaa cattgtgttt caaggtactg gaatgctttt tgaagtcgaa ggtaaggtta
    28261 agtcttcgaa gtttgaaaat ggtggtgaag gcgacacaag gttagaaatg tttaaaaagg
    28321 gattagaaca tttcggttta gaatataaaa taacgtatga caaaaagaaa gacagatata
    28381 agtttgtatt gacgcctttt gcaaatcaaa aagcgtctta ttttatttct gacgaagtca
    28441 acgccaacgc tataaaactc gaggaagatg caagtgattt cgccaccttc attagaggat
    28501 atggtaatta ttcaggtgaa gaaacattcg aacacgctgg gctcgtaatg gaagctagaa
    28561 gtgcattagc tgaaatatac ggcgacatcc acgcagaacc atttaaagat ggtaaagtga
    28621 ctgaccaaga aactatggat aaagaattac aatcgagatt gaaaaagtcg ttaaaacaat
    28681 ctttgtcttt ggactttttg gtgttaagag aatcatatcc agaagcagac ccacaacccg
    28741 gagacatagt acaaataaaa tctaccaaac taggtttgaa tgatttagtc cgtatagtac
    28801 aagttaaaac gattaggggt ataaacaatg taattgttaa gcaagatgta acgcttggtg
    28861 agtttaatcg agaacaacga tatatgaaaa aagttaatac tgcagctaac tatgtttctg
    28921 gattaaatga cgttaatctt tccaatccta gtaaagcggc agaaaactta aaatctaaag
    28981 ttgcatcaat agctaaatca acactcgatt tgatgagtag aactgatttg attgaagata
    29041 aacaacagaa ggtaagctct aaaactgtga ctacatctga cggcactatc gttcatgatt
    29101 ttatagataa atcaaacatt aaagatgtaa aaacgattgg aacgattggc gattctgtag
    29161 ctagaggatc acatgcgaaa actaatttca cagaaatgtt aggcaagaag ttaaaagcta
    29221 aaacgaccaa ccttgcaaga ggtggtgcaa caatggcaac agttccaata ggtaaagaag
    29281 cggtagaaaa cagcatttat agacaagcag agcaaataag aggagaccta atcatattac
    29341 aaggtacaga tgatgactgg ttacatggtt attgggcagg cgtaccgata ggcactgata
    29401 aaaccgacac taaaacgttt tacggcgcct tttgttctgc aattgaagtt atcaggaaaa
    29461 ataatccagc ttcaaaaata cttgtaatga cagctactag gcaatgccct atgagtggta
    29521 caacgatacg ccgtaaagat acggacaaaa acaaactagg gttaacttta gaggattatg
    29581 tcaatgctca gatattggct tgtagtgaat tggatgtacc agtatatgat gcctatcata
    29641 cagattattt taagccatat aatccagcgt tcagaaaatc aagtatgcca gacggattgc
    29701 atccgaacga gaggggtcat gaagttatta tgtacgaact tattaaaaat tattaccagt
    29761 tttacggata gaaaaggagg aagacatgga taacaaatta attacagact taagtagagt
    29821 tttcgattac agatatgtag atgaaaatga gtataatttc aagcttattt cagacatgct
    29881 gactgatttt aatttctctc ttgaatacca tagaaataaa gaggtatttg cacataatgg
    29941 agagcaaata aagtatgagc atttaaatgt cacaagtagc gtctctgatt ttttaacgta
    30001 tctaaacggc cgtttcagca atatggtact aggtcataac ggcgacggta tcaacgaagt
    30061 aaaagacgcg cgtgttgata atactggtta tgatcataag acattgcaag atcgtttgta
    30121 tcatgattat tcaacactag atgctttcac taaaaaggtt gagaaagctg tagatgaaaa
    30181 ctataaagaa tatcgagcta cagaataccg attcgaacca aaagagcaag aaccggaatt
    30241 catcacagat ttatcgccat atactaacgc agtaatgcaa tcattttggg tagaccctag
    30301 aacaaaaatt atttatatga cacaagcgcg tccgggcaat cattacatgt tatctagatt
    30361 gaagcctaat ggacaattta ttgatagatt gcttgttaaa aatggcggtc acggcacaca
    30421 caacgcctat agatatatcg gcaatgagtt atggatttat tcagcagtgt tagacgctaa
    30481 cgaaaacaac aagtttgtac gtttccaata tagaactgga gaaataactt atggtaatga
    30541 aatgcaagat gtcatgccga atatatttaa cgacagatat acatcagcga tttataatcc
    30601 tatagaaaat ttaatgattt tcagacgtga atataaagct tctgaaagac aacttaagaa
    30661 ttcgttgaac tttgttgagg ttagaagtgc tgacgatatt gataaaggta tagacaaagt
    30721 attgtatcaa atggatatac ctatggaata cacttcagat acacaaccta tgcaaggtat
    30781 cacttatgat gcaggtatct tatattggta cactggtgat tcaaaaccag cgaaccctaa
    30841 ttacttacaa ggcttcgata tcaaaacaaa agagttatta tttaaacgac gtatcgatat
    30901 aggcggtgtg aataacaact ttaaaggaga tttccaagag gctgagggtc tagatatgta
    30961 ttacgatcta gaaacaggac gtaaagcact tttaattggg gtaactattg gaccaggtaa
    31021 caacagacat cactcaattt attctatcgg ccaaagaggt gttaaccaat tcttgaaaaa
    31081 catcgcgcct caagtatcaa tgactgattc aggcggacgt gttaaaccgt taccaataca
    31141 gaacccagca tatctaagtg atattacgga agttggtcat tactatatct atacgcaaga
    31201 cacacaaaat gcgttagatt tcccgttacc gaaagcgttt agagatgcag gttggttctt
    31261 tgatgtactg cctggacact ataatggtgc tctaagacaa gtacttacca gaaacagcac
    31321 aggtagaaat atgcttaaat tcgaacgtgt cattgacatt ttcaataaga aaaacaacgg
    31381 agcatggaat ttctgcccgc aaaacgccgg ttattgggaa catatcccta agagtattac
    31441 aaaattatca gatttaaaaa tcgttggttt agatttctat atcactactg aagaatcaaa
    31501 ccgatttact gattttccta aagactttaa aggtattgca ggttggatat tagaagtaaa
    31561 atcgaataca ccaggtaaca caacacaagt attaagacgt aataacttcc cgtctgcaca
    31621 tcaattttta gttagaaact ttggtactgg tggcgttggt aaatggagtt tattcgaagg
    31681 aaaggtggtt gaataatggt agtagataat ttttcgaaag atgataactt aatcgagtta
    31741 caaacaacat cacaatataa tccggttatt gacacaaaca tcagtttcta tgaatcagat
    31801 agaggaactg gtgttttaaa ttttgcagta actaagaata ataagccgtt atcaatcagc
    31861 aaacataatg cgatgactag tattgtgctt aagacggata acttcgacga tgaacacggc
    31921 gcttatatta gtgatgaact tacaattgtt gatgcaatta acggacgaat gcaatacgtt
    31981 atcccaaatg agttcttaaa atacactgga cgcgtacatg cgcaagcata ttttactcaa
    32041 aacggtagca ataacgtaat tgtagagcgt caatttagct tcaatatcca gaatgatcta
    32101 attagtaatt ttgacggtaa aacaaagcta gtttatatca aatcaattca ggacttaaca
    32161 gaaagtgtta aagaagaagt tgaggactta aagaaaagtt tgagtgatac aaaatcgttg
    32221 gttactgaaa ttgatagtcg tattaatcaa ggtattcaaa gattagaaat caaacaaaat
    32281 gaagcggtac agatgattac aacaacacaa gacaaagccg ttcaatatat aaatagcgag
    32341 ttccagaaaa ttgttgataa agagcaagcg atttttgaac gtgttaacga agttgaacaa
    32401 caaatcaatg gcgctgacct tgttaagggt aattcaacaa caaattggca aaagtctaaa
    32461 cttactgatg attacggtaa agcgatcgaa tcatctgaac agtcaataga agctgtttta
    32521 agacacgcta actcatctat gattattcat attactaatg caaaagatgt gccagaaaag
    32581 gcggatatag gcacgttaga gaagcccgga caagatggtg ttgatgacgg ttcttcgttc
    32641 gatgaatcaa cttatacatc aagcaaatct ggtgtgttag ttgtttatgt tgttgataat
    32701 aatactgctc gtgcaacatg gtacccagat gattcaaacg atgagtacac aaaatacaaa
    32761 atctacggca cgtggtaccc gttttataaa aagaatgatg gaaacttaac taagcaattt
    32821 gttgaagaaa tatctaacaa cacactgaat caagctaaac agtatgtaga tggtaagtta
    32881 caaagtataa gttggcaaca acataagtta acagaacata acggtcaatc aatccaaaag
    32941 aacttatata acgccaaagg taatttagaa gcattgggcg ctgggaatta ttacgtaaca
    33001 agtgtgcctg atttaccagg tattgttgaa agttacgaag gctacttatc agtatttgtt
    33061 aaagatgatg caaataagtt attcaacttc acaccttcaa actctaaaaa agtttataca
    33121 cgatcaatca ccaatggtcg attagattca caatgggcta cacctaacga acataaaaca
    33181 gccgtgttat tcgacggtgc tgcaaacggt gtaggaacaa ggattaattt aaccgaagct
    33241 tatacaaatt atgcaattct attcataagc ggtacttatc caggtggtgt tattgaagca
    33301 ttcagtttaa cctctatacc aaatgcgatt caattaagta aaacaaatgt agttgactca
    33361 gacggtaacg gtggtggtag ttatgaatgc ttaataacta aagaaagtgg tacgacgtta
    33421 aaaatcgata acgatgtgta ccttgattta ggcagtaaaa caggttctgg tgctaatgca
    33481 aacagagtta cgataaataa aattgtgggg tggaaataat gaaaatcaca gtaaacgata
    33541 aaaacgaagt tatcggatac gttaatactg gcggattacg caatagttta gatgtagatg
    33601 ataacaatgt gcctattaaa tttaaagaag agttcgaacc tagaaagttt gttttcacta
    33661 acggcgaaat taaatacaat agcaatttcg aaaaagaaga cgtaccgaat gcatcaaacc
    33721 aacaaagtgc gtcagattta agtgatgagg aacttcgcgg aatggttgcg agtatgcaaa
    33781 tgcaggtggt acaagtaaac gtattaacaa tggaattagc tcaacaaaac gctatgttaa
    33841 cacaacagtt gactgaactg aaaactaaca aaacaagtac tgagggggac gtttaaataa
    33901 tgaagatgat ttatccaact tttaaagaca ttaaaacttt ttatgtttgg ggttactata
    33961 aaaacgagca aattaagtgg tacgtagaca agggtttaat cgataaagaa gaatacgctt
    34021 taatcactgg agaaaaatat ccagaaacaa aagatgaaaa gtcacaggtg taatgcttgt
    34081 ggctttttaa tttaacaaaa agtaggtggc gtaatgtttg g
//
LOCUS       DBNENW010000011        33295 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNENW010000011 DBNENW010000000
VERSION     DBNENW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.011"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(52..1179)
                     /locus_tag="AC1LW8_000412"
     CDS             complement(52..1179)
                     /locus_tag="AC1LW8_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HIP4772279.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPGGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1541..1918)
                     /gene="ygs"
                     /locus_tag="AC1LW8_000413"
     CDS             complement(1541..1918)
                     /gene="ygs"
                     /locus_tag="AC1LW8_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing post-transcriptional
                     regulator Ygs"
                     /protein_id="HIP4772280.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2333..2926)
                     /locus_tag="AC1LW8_000414"
     CDS             complement(2333..2926)
                     /locus_tag="AC1LW8_000414"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP4772281.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2990..3373
                     /locus_tag="AC1LW8_000415"
     CDS             2990..3373
                     /locus_tag="AC1LW8_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HIP4772282.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3504..5909
                     /gene="mnhA1"
                     /locus_tag="AC1LW8_000416"
     CDS             3504..5909
                     /gene="mnhA1"
                     /locus_tag="AC1LW8_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HIP4772283.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5902..6330
                     /gene="mnhB1"
                     /locus_tag="AC1LW8_000417"
     CDS             5902..6330
                     /gene="mnhB1"
                     /locus_tag="AC1LW8_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HIP4772284.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6330..6671
                     /gene="mnhC1"
                     /locus_tag="AC1LW8_000418"
     CDS             6330..6671
                     /gene="mnhC1"
                     /locus_tag="AC1LW8_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HIP4772285.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6664..8160
                     /gene="mnhD1"
                     /locus_tag="AC1LW8_000419"
     CDS             6664..8160
                     /gene="mnhD1"
                     /locus_tag="AC1LW8_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HIP4772286.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8162..8641
                     /gene="mnhE1"
                     /locus_tag="AC1LW8_000420"
     CDS             8162..8641
                     /gene="mnhE1"
                     /locus_tag="AC1LW8_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HIP4772287.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8641..8934
                     /gene="mnhF1"
                     /locus_tag="AC1LW8_000421"
     CDS             8641..8934
                     /gene="mnhF1"
                     /locus_tag="AC1LW8_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HIP4772288.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8912..9268
                     /gene="mnhG1"
                     /locus_tag="AC1LW8_000422"
     CDS             8912..9268
                     /gene="mnhG1"
                     /locus_tag="AC1LW8_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HIP4772289.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9517..10671
                     /locus_tag="AC1LW8_000423"
     CDS             9517..10671
                     /locus_tag="AC1LW8_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIP4772290.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     FTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSTFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENNSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10727..11101)
                     /locus_tag="AC1LW8_000424"
     CDS             complement(10727..11101)
                     /locus_tag="AC1LW8_000424"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIP4772291.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGHVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11120..12436)
                     /locus_tag="AC1LW8_000425"
     CDS             complement(11120..12436)
                     /locus_tag="AC1LW8_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HIP4772292.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12847..14322)
                     /locus_tag="AC1LW8_000426"
     CDS             complement(12847..14322)
                     /locus_tag="AC1LW8_000426"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HIP4772293.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14453..15661)
                     /locus_tag="AC1LW8_000427"
     CDS             complement(14453..15661)
                     /locus_tag="AC1LW8_000427"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP4772294.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFDKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16115..16474)
                     /locus_tag="AC1LW8_000428"
     CDS             complement(16115..16474)
                     /locus_tag="AC1LW8_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIP4772295.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16487..16723)
                     /locus_tag="AC1LW8_000429"
     CDS             complement(16487..16723)
                     /locus_tag="AC1LW8_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HIP4772296.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            17040..18104
                     /locus_tag="AC1LW8_000430"
     CDS             17040..18104
                     /locus_tag="AC1LW8_000430"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP4772297.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(18163..18486)
                     /locus_tag="AC1LW8_000431"
     CDS             complement(18163..18486)
                     /locus_tag="AC1LW8_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HIP4772298.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            18587..18829
                     /locus_tag="AC1LW8_000432"
     CDS             18587..18829
                     /locus_tag="AC1LW8_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIP4772299.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(19092..20267)
                     /gene="dltD"
                     /locus_tag="AC1LW8_000433"
     CDS             complement(19092..20267)
                     /gene="dltD"
                     /locus_tag="AC1LW8_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HIP4772300.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKTPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(20264..20500)
                     /gene="dltC"
                     /locus_tag="AC1LW8_000434"
     CDS             complement(20264..20500)
                     /gene="dltC"
                     /locus_tag="AC1LW8_000434"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HIP4772301.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(20518..21732)
                     /gene="dltB"
                     /locus_tag="AC1LW8_000435"
     CDS             complement(20518..21732)
                     /gene="dltB"
                     /locus_tag="AC1LW8_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HIP4772302.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(21729..23186)
                     /gene="dltA"
                     /locus_tag="AC1LW8_000436"
     CDS             complement(21729..23186)
                     /gene="dltA"
                     /locus_tag="AC1LW8_000436"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HIP4772303.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(23202..23354)
                     /locus_tag="AC1LW8_000437"
     CDS             complement(23202..23354)
                     /locus_tag="AC1LW8_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HIP4772304.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(23402..23512)
                     /locus_tag="AC1LW8_000438"
     CDS             complement(23402..23512)
                     /locus_tag="AC1LW8_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772305.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(23766..24725)
                     /locus_tag="AC1LW8_000439"
     CDS             complement(23766..24725)
                     /locus_tag="AC1LW8_000439"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIP4772306.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNQSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(24758..25537)
                     /locus_tag="AC1LW8_000440"
     CDS             complement(24758..25537)
                     /locus_tag="AC1LW8_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HIP4772307.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(25537..25971)
                     /locus_tag="AC1LW8_000441"
     CDS             complement(25537..25971)
                     /locus_tag="AC1LW8_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HIP4772308.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            26069..26335
                     /locus_tag="AC1LW8_000442"
     CDS             26069..26335
                     /locus_tag="AC1LW8_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HIP4772309.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(26414..26797)
                     /locus_tag="AC1LW8_000443"
     CDS             complement(26414..26797)
                     /locus_tag="AC1LW8_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HIP4772310.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(26927..27844)
                     /gene="lipA"
                     /locus_tag="AC1LW8_000444"
     CDS             complement(26927..27844)
                     /gene="lipA"
                     /locus_tag="AC1LW8_000444"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIP4772311.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(27928..29247)
                     /locus_tag="AC1LW8_000445"
     CDS             complement(27928..29247)
                     /locus_tag="AC1LW8_000445"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIP4772312.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLKTLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(29274..30101)
                     /locus_tag="AC1LW8_000446"
     CDS             complement(29274..30101)
                     /locus_tag="AC1LW8_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIP4772313.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(30114..30962)
                     /locus_tag="AC1LW8_000447"
     CDS             complement(30114..30962)
                     /locus_tag="AC1LW8_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIP4772314.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(31138..32205)
                     /locus_tag="AC1LW8_000448"
                     /pseudo
     CDS             complement(31138..32205)
                     /locus_tag="AC1LW8_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
     gene            complement(32219..33259)
                     /locus_tag="AC1LW8_000449"
     CDS             complement(32219..33259)
                     /locus_tag="AC1LW8_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIP4772315.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT        10501 a         5966 c         4983 g        11845 t
ORIGIN      
        1 aaccactaac aaattgcata cacaatatca ttagtggtaa cagttaaaca cttatttatc
       61 tttacgtggt aatggataga atccttcatt aaattgatgc cataaattag gagttaagtg
      121 atgattatct tctctctctg gatcaaagta attaataatt tcatcttccc gtccatcttt
      181 aattttttca acaaattgat aatccagtag tagctctcta ccaatggcta ctaagtcaac
      241 accaacattt tcaactgcat ctaaagcttc gtcagctgtg aaaattgaac caataccgat
      301 aagtggcata cgaccattta tccatttgtg aattaaaggc agtctttctt gtccagcgta
      361 tttaccttca cgtgtcgttg catgcgtatc catcatcgaa acatgaatat agtcgattgg
      421 catatggcta attttattaa cgagttcctc tgtaatttcc attgtgattc ctggagactc
      481 cgcttcctct ggagataatc tgtatccaac tataaagtct ttattgccat acgcttcttt
      541 cgctttaagc acctcttcaa tcacagcgac cgggaattta tattgatttg cccaaacatc
      601 atttcttcta ttatagtatg gtgatacaaa ttgatgaatt aagtaatgat tcgcgccatg
      661 tatttcaaca ccatcaaatc ctgcttcaat tgcacgtcgc gttgcttcac caaaatcctt
      721 gattgcttgt tcaatctctt cattcgtcat ttctctagca ctatgttctt gtttctgacc
      781 gaaacttttt aaagaaattg gacttggtgc tacgacgtct ccaccaggtg ttaattcagg
      841 caatgcttgt gcaccgccat gatgtatttg tacgagtgct ttggcaccgt ttttcttcat
      901 tgctgtagct aatcgtttta gtccttcaat atcactgtca tgcgcgattg atggctgtcc
      961 tggaaatgct tttccgacat cactcacatt actcgcagca ttaattgtaa taccaacatc
     1021 ttgtgaacgc ttttcaatat aaggaagttc tacatctgaa atagtaccat catcatttga
     1081 agaaatatgt gttaaagggg ctaacacaaa tcgatttctc aactctactc catttggtaa
     1141 ttctacttta tcaaacaatg gttcgtattt acttttcatt acttaattcc tccttaaaat
     1201 tattgagttg actataacat gtaatttttt cattctccaa ttatctgttt atatagttgc
     1261 aattcgttta atactttaag ttgtagttct catttctaaa ataaattaaa aaaacaaatc
     1321 cacatacaat attgcaaatc aatgattcta tctatcaaag ataatggata tgtcacctta
     1381 aaccatttgt acgtgtaaat agaatcaatc atattacgtt atcgtagaca aaaagagatt
     1441 agtatctaat aaaatttaat agatactaat ctcttttata gtctaagaaa ctttcatttc
     1501 agaccctttg tatgattttc ggtacgattt atctgttcct ttagtcgttt cgaattgctc
     1561 gctttgactg ttttatccag attggtaagc gttctttaag tgtttgaaac ccatattttt
     1621 ctgtttctct gatttcatct aatactgatt gtttttcctt cttacgctca taatttttga
     1681 agtaatcatt atcctttaat gatagattaa gctttccttc atcatctata gacaaaattt
     1741 tagctttaac aatttggcct tctgatagaa atttcttcaa attatgaacg tagtcatcca
     1801 taatttctga tatatgaatc agtccttcag tatgattagg ggtctcaaca aacgcaccgt
     1861 atggttgaat accagttaca cgcaccttga tatgttggcc aattttgtag ttattcaact
     1921 taataacccc ttatttcgtt tacttaacac tattatcata gcacaaatcg aaatggtgga
     1981 ctaattaaaa catcaaatca tacgaatatt attttataaa tgaataagaa ttctacaaac
     2041 ttggatttac aaataaaatt agcaacaaac tcctatctaa atcaatatga tatccagcta
     2101 ctttgttttc aatttattaa ctatttgaat agcaattctc tttaaaaaca taaaaagcaa
     2161 cctcaactat tatctttaga atacacatct atttaaaaac tgtgctttct tgataaatat
     2221 attgagattg ctcatcatgt tagtttattt gtttcgttgg aatatatact aatccattca
     2281 taaattagct ttatccgagt ttaaaatgtt ggtagttaat tatgtttagc tattattctt
     2341 caacatcaat agattcaatt acaacatcat gaagtggttt gtcttgtggt cccacttttg
     2401 tatttgcaat atcttctaaa gtagtttcac catcaatgat ttgaccgaat actgtatgtt
     2461 tttgatctaa ccatggtgta ccaccctttt cgccatatgc atcaacgatt ggttgaggcc
     2521 agccaccatc tgcaagttga cttaacatat tttgaggtac ttctttcatt tgaacaatga
     2581 aaaattgtga accattagta ttaggtcctg agttagccat tgataatgcg ccatataagt
     2641 taaatgcatt taatgaaaat tcatcttcaa aagcaccgcc ataaatactt tcgccaccca
     2701 taccagtagc tgttggatcg ccaccttgaa tcatgaagtc attaatgaca cggtggaatg
     2761 ttattccatc ataataacca ttttttgcat gtgtcacaaa attttcaact gtttttggtg
     2821 caatatttgg aaataatttg aatgtcatgt cacctttatt tgtgtgcata accactttaa
     2881 tttcaccttg ttgtacttct ttgtttaact gtggatagtt agccatttat attctccatt
     2941 catgttatga tacatatata ttaacatact tttaagggag gaaaatagca tgaagctata
     3001 tcgattttca cacaaaacag gtagctatgg tgtcacgatt gttgaagaaa atggagacga
     3061 tgtccttgtt aaagttgaac aagttatcaa acacccgaaa caaggtgatt tacataaccc
     3121 tactgaaact gaaggtgtat ttttccatga acgcaaagca ttaagccatt ttgaaaaacg
     3181 ctatacgaaa cgttcacaat tacgcgactt caatgttgat gtattgcgct atgaagattc
     3241 acttcaacaa gcaattacag atttagaaac taaacttaaa gctcagcaaa caaaacatgc
     3301 tgaaatgtct ttagctagtt tagcacgctt aaaagaagat tattcacttc aatacaaaga
     3361 aaattttaaa taaaaaatga cgtgtttctt cttatttaaa tacttctgaa aataaagcac
     3421 aacaaggagt gtgtacgttt ttcagaacta tatcattcat gtataatgaa taacataaat
     3481 attttttaaa ggaggacaac atagtgagtt tgttacatat tgcggtgata ttaccgttaa
     3541 tattcgcact cattattcct atcctgtata gattttttaa acgtatacat ttaggatggt
     3601 tcgtattacc tgtccctatc gtcatattta tttatatgct tacattaatt aaaacgacga
     3661 tgtcaggtaa tactgtaatg aaaaccttaa attggatgcc tcattttggt atgaactttg
     3721 atttatatct tgatggctta ggtttactct ttagtttgct tatttcagga atcggcagtt
     3781 tagtagtatt atattcaatc ggctacttaa gtaagtcaga acaactaggg aatttttatt
     3841 gttatttatt actatttatg ggtgcaatgt taggtgtcgt actttcagat aatgttatta
     3901 ttctatattt attctgggag ctaacgtcat tttctagttt tttacttatt tcattctggc
     3961 gcgaacgaca agcatctata tatggtgctc aaaaatcgct cataatcaca gtatttggtg
     4021 gtctatcatt attaggtggt atcatcttac tcgctatacc aacacaatca tttagcattc
     4081 agtacatgat tcaacatgct tctgaaattc aaaatagccc attcttcatt tttgcgatga
     4141 ttttaatcat gattggtgct tttacaaaat ctgctcagtt tccattttat atttggctac
     4201 cagatgcaat ggaagcacct acaccagtca gtgcttatct tcattcagca acaatggtta
     4261 aagcagggtt atatttaatc gctcgaatga caccaatctt cgcagcatcg caaggttggg
     4321 tatggactgt cacactcgtt ggtttaatta cattattctg ggcttcgcta aacgcaacaa
     4381 aacaacaaga cttgaaaggt atactggcat tttcaacagt atcccaatta ggtatgatta
     4441 tggcgatgct tggaatcggt gccattagtt atcactatca aggcgatgat agcaaaatat
     4501 atgctgctgc atttacggct gcaatattcc atttaatcaa ccatgcaaca tttaaaggtg
     4561 ccctatttat gattacaggt gcagtggatc attctactgg tacacgtgat gttaagaaat
     4621 taggtggctt actaacaatt atgcctattt catttacaat cactgttatt acagcattaa
     4681 gtatggctgg tgtaccaccg ttcaatggtt tcttatcaaa agaatcattt ttagaaacaa
     4741 catttacagc aagccaagca aatttattca gtgttgatac attaggctac ctattcccaa
     4801 tcattggtat agtgggcagt gtcttcacat ttgtatattc aattaaattt ataatgcata
     4861 tatttttcgg acaatataaa cctgaacaat tacctaaaaa agcacacgaa gtatcaatat
     4921 taatgctttt atcaccagct attttagcta cattagtaat tgtatttggt ttattccctg
     4981 gcattttaac taattcgatt attgaaccag ctacatcttc gattaatcat acggtaattg
     5041 atgatgttga attccatatg ttccatggat taacgcctgc ctttttatca acattagtga
     5101 tttatatttt aggtatctta ctaattgtga catttagtta ttgggtgaaa ttgttgcaac
     5161 gacaacctgg taaactaaca ttcaattatt ggtacaacag aagtgcaaat gttattccaa
     5221 actactctga aaagatgacg aatagctatg ttaccgatta ttcacgtaat aatttagtta
     5281 tcatttttgg tgcattaatt ttattaacat ttgtcactat attctcagta cctttcaaca
     5341 ttaattttaa agacgttagt ccgattagaa ttttcgaagt ttgtatcgta attttattat
     5401 taagtgctgc ctttttaatt cttttcgcta aatcaagatt atttagcatc attatgttaa
     5461 gtgcagttgg atatgctgtg tctgtattgt ttatattctt taaagcaccc gacttagcat
     5521 tgacacagtt tgtcgttgaa tctatttcaa cagcattgtt cttattatgc ttctaccact
     5581 taccaaattt aaatcgttat aatgaaaagc gctcattcca gcttactaat gctttaattg
     5641 ctggtggtgt tggtttgtcc gtcattatta tcggtttaat tgcatacgga aatagacatt
     5701 ttgaatcgat atctaagttt tatcaagagc acgtttatga tttagctcac ggtaaaaaca
     5761 tggtaaacgt catacttgtt gatttccgtg gtatggatac tttattcgag tcatctgtgc
     5821 ttggtatcgc aggattagct gtatatacaa tgattaaatt acgtaaaaag cgtcaaacgc
     5881 aaggtaacga ggtgaaaaat catgaataga caacaaaatg atctaatttt gcaatttgct
     5941 gcagttatta tctttttcat ggtaatggtc tttggttttt cacttttctt agcaggacat
     6001 tatacacctg gtggcggttt cgtcggcgga ttattattcg caagttcact tgttatcatt
     6061 acgattgcat ttgatataga aacaatgcgt aaaattttcc cattagattt taaaatcttg
     6121 ataggtatcg gattagtttt ttgtattgcg acgcctatcg caagttggtt tttaggcaaa
     6181 aatttcttta cgcatgtcac atttgatata ccgttattta tattagaacc tgttcatatg
     6241 acaacagcag tattctttga tttcggtgtt ttatgtgcag tagttggtac tgtcatgaca
     6301 attattattt cgattggaga gaacgaatag tggaaattat tatgattttt gttagtggta
     6361 ttctcacagc aattagtgtc tatctcgttt tgtctaaaag tctgatacga attgttatgg
     6421 gaactacact attaacacat gcagcaaatt tatttttaat aactatgggc ggacttaaac
     6481 atggtactgt tccaatttat gaagcgaacg taaaaagcta tgttgatcct atcccgcaag
     6541 cacttatttt aacagcaatc gttatcgcct ttgcgacaac agcctttttc ttagtattag
     6601 catttagaac atataaagaa ttaggcacag ataacgttga gagtatgaaa ggagttccag
     6661 aagatgattg aaagtaatat gcttgtgtta acgctagtta ttccagtaat tacagccata
     6721 ttacttgtct ttattggaaa gcgtcctatt ataaagcgtt atgtcgcact aggcggtaca
     6781 ttattaacct tggtcgcggc aattatcaat ttagcaaatg ttgttaaaca tggaccaata
     6841 cgcgtagagc taggttcatg gaaagcacca tacagtattg tctttgtatt agacatattc
     6901 agtgccctac taataattac tagtattatt attacagcta ttgtgatttt atattcatac
     6961 caaacaattg gtattgaacg tgaaagatat tattattatt tttcagtact atttatgtta
     7021 ataggtatca ttggtgcatt cacaactggt gacattttca acctattcgt atttttcgaa
     7081 gtatttttaa tgtcatctta tttcttatta gtaattggtt ctacaaaaat tcaactacaa
     7141 gaaacaataa aatatgttct tgttaacgtt gtatcgtcat ccttcttcgt aatgggtgta
     7201 gcgattttat attcagtagt aggaacattg aatctagcag atataagcaa taaattagct
     7261 aacctttcag cgcatgacag tggcttagtc aatatcgttt tcatactatt tatctttgtt
     7321 tttgctacaa aagctggcgt attcccaatg tttgtttggt tacctagtgc atactatgca
     7381 ccaccaattc caatcattgc attctttggt gctttgttaa caaaagttgg tgtttatgca
     7441 atcgctagaa cattgagcct attctttagt gataatgtaa gcttttcaca ttatgtcatt
     7501 ttattcttag cactattaac gatcatcttt ggttgtgtcg gtgctgttgc atatgccaac
     7561 attaaaaaaa tcatcctata caatgtcatg attgctgtag gtgtgatctt agtcggcgtt
     7621 gcaatgatga cagagtcagg tatgattggg gctatttact atacattgca cgacatgtta
     7681 gtaaaacttg cattattctt gcttatcgga attatgatta aaattacagg cactgctgat
     7741 ttaagacaat ttggtggctt aattaaacga tatcctgtac ttggttggtc attctttatc
     7801 gcagcattaa gtttagctgg cattccccca ctcagtggct tttatggaaa attttttatt
     7861 gtccaatcaa catttgaaag aggattttat ttaagtggtg tcatcgtctt attatcaagt
     7921 ttagtcgtat tatattctgt cattcgcata ttcctacaag gctttttcgg tcaaccaaaa
     7981 ggatatgatc ttaataacaa agtcgacgtc aaatatttaa ctacgattgc aatcgttgca
     8041 gttgtgataa ctgttttata tggattgtca gcagattact tatatccaat ggtaaaagct
     8101 ggtgcagaaa cattctacaa tccatcaacc tacgttaaag ctgtattagg aggtaaataa
     8161 catggctgta caattagtat taaactttat tatcgcagtg ttttggttgt ttgtgacaaa
     8221 tagttacaca acaaataact ttgtgttagg tttcatcttt ggcctagttc ttgtttacct
     8281 cttacacaga gtgttaccag gtagatttta tgtcatcacg ctatatcgta ttattaaatt
     8341 agttattatt ttcttaatcg aactgattaa agctaatttt gatgttttga aaattattat
     8401 taaaccttcg attaaaaatg aaccaggatt ctttgtttat cacacagatt taaaaaaaga
     8461 ttggcaaatt gttttgctgt ctaatttaat tacattaaca cctggtactg tagtgcttgg
     8521 tgtaagtgac gatcgtacaa aaatttatat tcatgccata gatttttcaa ctaaagagca
     8581 agaagtagaa agtattaaaa catcattaga gaaaattgta agagaggtgg gcgaaatata
     8641 atgaatcata atgttattat cgttattgca ttaatcatag ttgtcatttc tatgttagct
     8701 atgctcattc gcgttgtgct aggcccatca cttgccgatc gtgttgtcgc attagatgcg
     8761 attggtcttc aattaatggc agttatagca ttattcagta ttttattaaa tattaaatac
     8821 atgattgtcg ttattatgat gattggtata ttagcttttt taggtactgc agtattctct
     8881 aaatttatgg acaaaggtaa ggtgattgaa catgatcaaa atcatactga ttagtcttgc
     8941 acttatcttt gttatcatcg gtgctttaat tagcgccctg gcagctatag gattattgag
     9001 acttgaagat gtatattcac gtgcacatgc tgccggaaaa gcatcaacat taggtgcaat
     9061 gtcattacta tttggtacgt ttttatattt tattgctact caaggttttg taaatatgca
     9121 attaatcgtt gcgattatct ttgtattaat tacaggtcct ctttcaagtc atatgattat
     9181 gaaagcagca tataatatta aaacgcctta tactaaaaag actaaagtcg atgaaatttc
     9241 agaagactta aaagacacaa aattgtagat tatataacaa aatcatgaat ataaatcaac
     9301 aacaaacagc agtaagatga ttccaaatta ggaatgattt tactgctgtt ttcttttgac
     9361 attgttacct ctttttcaat ggttttttct ttgactacag cttcgcccta tctacatata
     9421 tctctttaat ttaattgcct ttcatgtcgt tatgtattat gataataata attataaatc
     9481 gtaacgatta cgctttaaaa agagagaggt tttattatgc attggacaat tatcggcggt
     9541 ggcatacagg gaactgcaat cgcacaaaaa ctattatcaa gcggattaac aacagaccga
     9601 ttaacaatca ttgacccaca cgaaactttt tgccaaaggt ttaactcatt tacaaatcga
     9661 atagaaatgc cttatttaag atcaccgatt gtacatcacg tacatccaca accgttccat
     9721 ctaaaacaat tcgctaaaca gcaccaatat acaaatgctt tttatggtcc ttatcaacga
     9781 cctgaattga caatgtttat ggatcatatt gcacatgctt ctaaacaata tcaattagag
     9841 gattgcttgg ttcaaggttt agttcaaact ttagataaac aagaagacaa atggcatatc
     9901 aagttagaag atggacaaat tatcactaca gattgcgtcg ttattgcaat aggtagtaca
     9961 aatattccgt ttatgcctga cattttaaaa gacaaacaga atgtaaatca tatcttcgag
    10021 aaagaacatg atcaagtagt atatgataag accgatcata tcgttggtag tggcattacg
    10081 gctgcacatc ttgcacttaa attgttaaat catgataacg ataaaaagat tcatttatgg
    10141 ctaaataaag atattgaaat acatgacttt gatgctgatc ctggttggtt aggtccgaag
    10201 aatatgtcta catttttaag tactaaaagc atgcctgaaa gaaatgccat tgtacaacgt
    10261 gaacgtcata aaggatcaat gcctcacgaa ctgtacttac gccttaaaaa acatattaaa
    10321 aatggtcgta taaatgtgca taaaacacct atcactcaaa ttagtgatgg tttaattaac
    10381 actgaaaata attctgttcc atatcaacag attatggttg caactggttt tgaacaagat
    10441 tttatgtcac aaccacttat taagcaatta atacaaaatt atgatgcacc tatcaacgaa
    10501 tgtaattacc ctgttatttc cgaaaaatta gaatggatac caaatctatt tgtcgcagga
    10561 tgctttgcag acttagaatt aggaccattt ggtagaaatg ttatgggtgg ccgtaaagct
    10621 gccgaacgca ttgaacaagc atttctaaaa ttacaacaat atagcgcata aaaaaactgc
    10681 tagccgtagt tatgaatatc tcataacatt tcagctagca gttcttttat tttaaaggct
    10741 taatagcaac tgtaccacgc ataactgtaa ttaattgttc tttgtcattc ttaattttta
    10801 tatcccatac atgtgtcgac ttaccttgat gaataatttc agctgtcgca gtaacatgac
    10861 catctttagc agaatgaata tgattggcat tcatctctaa acctaatgga ataaatttgg
    10921 ttgtatcaat tagattagca gatcctaatg aacatgctgt ttcacctaaa gcaatcgaag
    10981 caccaccatg taaatatcca aatggttgtt ttactttatc agtaacaggc atagaaataa
    11041 caactaaacc atcttcctgg tgatctattg acatctcaaa tgtctctaat aaatgagtca
    11101 tattttcacc tctttatgct tatagtacca tagcagcaat agtaccgaaa atcattaaag
    11161 gaatattata aaacaagaag tttggtacac atgtatcgcg tatatgatca tgttggccat
    11221 caacatttaa tcccgcagtt ggtcctaatg ttgaatcact tgcaggtgaa cctgagtcac
    11281 ctaatgcact cgctgttcca atcaatgcga ttaatgccat tgtatctagt ccaattgacg
    11341 ctccaaaagg aatgaataat gatgcgataa taggaattgt tgcaaatgat gatccaatac
    11401 ctaaagtgac aattaaacct atcacataca tcatgataat gctaaataat ttattatcac
    11461 cagtaatact tgttaaagtt ttaactaatt catctatatc accagtagca ttcattacac
    11521 cagcaaatcc atttgctgtt aaaataacta caccaatata agccataatt ttaatacctt
    11581 caacaaactt agcatctaat tcataccaat tatatgcacg tgaaataaag aatacgagta
    11641 cccctgccag tgcaccaaaa atcattgaat ctgtaaatgt ttgtactaaa aatgtagcta
    11701 gtattgctac aattgttact attaagatat atggttttaa ctctgtaaca ttgtcactat
    11761 ctgaaatttt acgtgtttca tattcacgtg gtttacgata tacatataaa ccgataagta
    11821 agccaacaat ataccccatt gaaggaataa gcattgcttt ccaaatcata ttaaactcaa
    11881 ttgggtgatt cgcctttgca aagccacttt gaataatttg ctggaaaatt tgaccgaatc
    11941 catatggtaa taacacatac gggaaacata aaccaaaacc gataatcaaa ccgattaaac
    12001 gtctatctat ttttaagtca ttaaacagac ttaacaatgg tgggatgaca attggaatga
    12061 atgcaatatg tacaggaatt aagttttgac tcatgatact catagctaat aatgcaatga
    12121 ttattgtcac tttgacttta actcttgacc atcgactatt ttcagcgtga attgcattta
    12181 taatttttcc tactaaatag tctgtgatac cactgtatga aattaatgct gcaaatccac
    12241 ctaataaagc atagcttaat gctacctcag caccatcgac tatatttttc ccaaatacat
    12301 ttataacttt ttcaatgctc atgcctgaaa ttaagccacc aactagcgca ctgataaata
    12361 agcttataac tacgtttaat cgacataaac atagcacaat cattaaaatt actgctatta
    12421 ctactgcatt tatcatatga caaccatcct ttatcactct atcgaactaa agcgtttata
    12481 gactacatag tatcagtagc tttaacacct gtcaatagca aactatacca ataatcttta
    12541 ttttttagtt aattgtcttt cttaaaataa ttttagcttt cattaaatta aacaatttta
    12601 caagcttgga acaccaatca aaatcctaag ttctaaaatg caatattagt agtcgttgac
    12661 tgaatgaaca tatgcttata atattttttt gcaatgctag tcaagttgat ttatgctcac
    12721 aaggatatgc gatttatatt ttcttacaac aatgaaaatg cctgatacaa tgcgatcctt
    12781 tatactttca agagttaaat tagaaatgta tatcaagaca caagttgtat caggcaaata
    12841 attaaattat tgttgtttta accattgtac tatcgtcgga atcataaatc ctgttggccc
    12901 ttttggacca ttatatgaag ctttattagt cgttgctgga cctgcaatat cgaaatgaat
    12961 gtgaggtgtt tgaccactaa aatgtgttac aaaacttgcc gcaaatagcg ctttaccttg
    13021 tccattcgta tggttaacta aatcagcgat atcactgtgt ttaatacttg cacgttcggt
    13081 tgcagtaatc ggtaattcaa ataccatttc atcgacttca gaacttattt gtaatatatc
    13141 gtttaatatc actttactat tcgattcaaa tgcagcagct ttatcatcgc ctagtgcaac
    13201 aattgctgca cccgttaatg tagcaaagtc cataatcaca ctaggctgat attgatttgc
    13261 ataaaacaca gcatctgcaa ggactaatct accttcagcg tctgtattca ttacttctac
    13321 agtttcacca cttaatgctg taaatacatc atctggcttc attgatgctt catttatcat
    13381 attttcagca cacgcaagca ctccgacaat atttacaggc agttgtaaac gactagccgc
    13441 ttcaatgata ccaacgacat tcgcagcgcc acacatgtca aacttcattg tagccatgcc
    13501 attcttcgtt ttaatactat aaccaccaga atcatacgtt atacctttac caactaaggc
    13561 aattggtgct tcatctttgt ctttgccatt atatgtgatg gttactaatc tcggtttatg
    13621 cttactacct ttacctactg cttgtaaaag tccgaatcct tcagaaacta aagtgtcata
    13681 atctttaaca tctactttga cctttgtatt tttaaaatga ttaacaatat cttctgcaaa
    13741 tgtttgtggt gttaatacat tcggtggcat attactaaag tctcttgcca aattaatgga
    13801 ttggccaata ctgataccct catgaataaa atctaattca attaatgatt cactaataag
    13861 gtttaaattc gtcttaaacg gtgccttctt acttgattta taatgatcga attcatatgt
    13921 tgcacgctca ctttgaatac cgcatgccat taatacatct gataactgat catattttga
    13981 aataaatgaa tccataagta aatacgtatc ttcaatgtgt tctgacttta tgtattggaa
    14041 aagatgtccc catattttca acatatcttg ataactacgt gtctttaagt ttcctaaacc
    14101 aactgtaatt aaacgatatg tttgatcttg tacatcaaat gcagttgtat aaatcttccc
    14161 aactttacta ccaataatat gttgatgttt tagtctttca agtgattctg taatatcgat
    14221 atgattaaaa ctaatgcgct ctaactgatt taaatgttct ggtataccaa taatcaatgt
    14281 atttatttcg ttgcttagtg tgttatttaa tttaaaattc ataatgtacc tccttagatt
    14341 tgatatgtta ctcacttcaa attgtacaac aataaagccc ttcagtgaca ctgaaggact
    14401 ttataaatga ataatttaat tttatgtgtt tagctgaaac tttatttaaa gcctagaatt
    14461 tacctttttt gaatgctaaa ccgataccac cgattttgaa taccgcacgt gtatcaatca
    14521 cttttttcat gaatgctgct tttttaccag cgataggttt atcaaatacc atacctacac
    14581 cgtcatgtga acctaaagaa caaacagttc cacgatcaac gtattcgaat tcttcagttg
    14641 attcaccgtt taagatgcgt ttaatgtttt tagcaacact ttcaccttgt tgcattgcaa
    14701 tttgtgctgt agttggtaat ggacgttctt ctccagctgg gataaacgct gaacagtcac
    14761 caataacaaa aatgttgtcg taaccattga ttgttaaatc ttgctttgta acgatacgtc
    14821 cacgtttaac gccttcaaat gattcttcca ttaatttact accacgtaca ccagctgccc
    14881 atactgaagt acctgcattt aattgttgtt tttcaccatc tacttcaact acaaaacctt
    14941 tttcgttaca agcaacgatt ggtgtagcaa ttttaaattc aacaccgcgg tcttctaagt
    15001 agctaactgc gtggttaact aattcttctg agaacattgg taacatttta ggtgctgctt
    15061 caacacaagt gattttaact ttattttgat ccacaccata tttgctacat aattcaggaa
    15121 ttctgtctgt taattcacct aagaattcaa caccagtgaa tccagcacca ccaactaaga
    15181 tagataaatc gttatcatct ttttcttttg atgctgcata gttagcaaat ttgtcttcga
    15241 tatgacgtga taattcacgt gctgtgataa cattttcaat ttggaaagca tgatctttca
    15301 taccttcgat gccgaatgtt tcactaacga aacctaatgc tactactaaa atatcaaagt
    15361 cataaatacc ttgatttgtt tctacctttt tagcatcacg gtcaattttt gttacttctg
    15421 cttgaacaaa gttcactttg tctttcttca agacactttc cacaggatat aatacatctt
    15481 catagtttag tgtacctgct gatgcttcat gtaaccatgt tgcttcatag tgatattcat
    15541 ttttattaat aagcgtaatt tctgcttctt ctgttgatat cgctttttgc aatttagtta
    15601 cagtttgtaa acctgcataa ccagcaccaa gtacaagtac ttttttacga tcttgagcca
    15661 tttaatttca cctaagcttt catatttttt taaccaaatg ctgataatta ctagtaacaa
    15721 ctggtcatta ctacttttac cacaatgtat actagtttat catcattttg ttatttaagt
    15781 cttttaaatt atgtaaaaac ctgtgatgtt cacaaaaaag acatttttaa tccaatacta
    15841 attctatagc ttttatgtta tattttcaag caagattcag tcagattgtg aaaaaattaa
    15901 ttaatttttt cgtagcctga tttcgattaa aaatgtatcc gctatcacga tcatttaaaa
    15961 tactatttat agtcagattc ttaattttca ataagctttt taataaaaca aaatatcttt
    16021 acgcaaaaat aggacataat caaatatgtc cttaatcgtt attgatatca atttgtatcc
    16081 caaaaattgg gttaaccatt aaaaagttat tttattagca attttcagga ttacctgcaa
    16141 cttttgcagt tctaaatgaa ctaccacagc cacatgaagc aattgcatta ggattgtcga
    16201 tttggaaacc gccacccatt aatgattgct taaaatcaat agtcgtacca tttaatacgg
    16261 gtgcatcttt tttgtctact aatactttta atccaaagta ttctaagact tcatcatttt
    16321 caccaggcgc ttcttctgca cccataccgt atgttaaacc agtgcacccg ccaccattca
    16381 ctttaatttt taaatagcca tctggcattt catttgcttt aagcatatct tttacttcgt
    16441 aagcagctgc ttctgttaat ataactgttg gcatgataac tcctccttaa aaaatccaag
    16501 tttcttttat atgtgcatat atattttgta ataattcttc cggcgaatca ccttcaacaa
    16561 tatcaccatt tactaaagca tacaacccgg ctgaacatat accacaatgt gtcaggcaac
    16621 catactctaa cacatcgaca tctgggtcat tttccagttg attaaaaaca taatctccac
    16681 cttttgccat gttagagaga caaaattcta cgatcggatt catacttcac cttcttattt
    16741 catttgttac aatattatag cattttaaaa ctggtatttt aacatgatgt gctcaattag
    16801 caacaactga tgtttcttat cccagttatg taatagtgcc ttagttagta caaatattta
    16861 tttgtagtca ttttcaaaaa aatatataat aagcatataa atacatattt acttttttag
    16921 gggttattga tgaatcaaag acacttgcat tttataactg ttcattaaca aatatatcta
    16981 tgcactttga tcttttcaaa attaaagtaa atattaacat ataaaagggg tacttcatta
    17041 tgaaaaactt agttttgtta ggcggcggat atggtaatat gcgtatcatg tcacgcattt
    17101 taactacttc tttaccacaa gattatacag tcacattagt tgatcgtatg ccatttcatg
    17161 gattgaaacc agaattttat gctttagctg cgggcacgaa atcagataaa gatgttcgta
    17221 tgaaattccc taatcatcca caagtgaata cagtttatgg tgaaattaac gacatagatt
    17281 tagatgctca aattgtctca gtcggtaatt ctaaaattga ttatgatgag ctaatcattg
    17341 gtttaggatg tgaagataaa tatcataacg ttccaggagc cgaagaatat acacatagta
    17401 ttcaaacact ctcaaaggct cgggatactt tccatagtat tagtgaacta ccagaaggtg
    17461 ctaaagtcgg tatcgttggt gctggattaa gcggcataga acttgccagc gaattaagag
    17521 aaagtagatc agacttggaa atatatcttt atgaccgtgg gccgcgaatt ttaagaaatt
    17581 ttccagaaaa attaagtaag tatgttgcga aatggttcgc caaaaataat gttaccgttg
    17641 ttccaaattc aaatattaat aaagttgaac ctggtaaaat atataactgt gatgaaccta
    17701 aagatattga tttagttgta tggacagcag gaattcaacc tgttgaagtt gttcgtaact
    17761 tgccgattga tataaatagt aatggacgcg tgatagttaa ccagtatcat caagtaccaa
    17821 catatcgtaa cgtctatgta gttggtgatt gtgctgattt accacatgcg ccaagtgctc
    17881 agttagccga agttcaaggt gatcaaattg ccgatgtgct taaaaagcaa tggctaaatg
    17941 aaccattacc tgacaaaatg ccggaactaa aggtacaagg tatcgttggg tcattaggag
    18001 ataagcaagg ttttgcatat atcatggacc gcactgtaac aggacgactc gcttctattt
    18061 taaaatccgg cgtactttgg ttatataaat atcataatgg ttaaaattaa aaagcaatgt
    18121 cagttcacac tattactgac attgctttta gttatgtgta tattattcat tcacaagctt
    18181 ttggtctata aatcgagtga tttgctttgt ttgtatataa ccatctgcta catattcatc
    18241 gttcattgta attaatggat aaaatagttc atcttgttct attctttcaa taaattgtaa
    18301 atcatgatct gttaagttgt cattatcttt tgtaatatct atatacgtat atttaaatga
    18361 tatatttggg tattttcttt ttaatagcgg ctgtagccag tcataaatat ctttcgatgt
    18421 tggcgcatta acgcaacttg cacatataac atctgcccca tatactacca cactcacgtg
    18481 ctccatatat ttatccccca ttggtttgat agatttttat tacactatct attataatat
    18541 actaataaag attatcaaat tcatctctcg aaaggagact tgcctgatgc ctactgaaga
    18601 tacaacgatg tttgatcaag tagcagaagt tattgaacgt cttcgtccat ttttattacg
    18661 tgatggtggc gactgctcat tgattgacgt ggaagacggt attgttaaat tacaattaca
    18721 tggtgcatgt ggtacatgcc caagttctac aatcactctt aaagctggta ttgagcgtgc
    18781 attacacgaa gaagtgcctg gtgtaataga agtagaacaa gtattctaat ataaagatat
    18841 taagttaaca gaacatatta tgaataacaa caaagacagg aatactgaat tatttataat
    18901 cagttattac ctgtcttttt tcgctgtcaa attggactga tgggaaagtt attttatact
    18961 atttatctga ttaattattt tattattgcc acatatccaa gcacatatta ctattctatt
    19021 ctatgaaatt taaaatataa aaatagcaca tacgctcaat caaaatcgtt gagttatgtg
    19081 ctatttgtat tttaattttt aggtttatct acttcaggtt gtggttcacc tttcatatgt
    19141 ttcgcaattt gctcatccat ataaacccaa cctttccaac cgatgtgtac ggcatcactg
    19201 ataacatatt tttcataatc tttatcagtc atatcgtaaa ttttaccacc attatctaca
    19261 acagtagaat ggattttttt ataaactgct tgacgacgtt ctttatcgat accaatgtgg
    19321 tcataccata caccgtttga tggaatactt acatattgaa catctgcacc agcagcacgc
    19381 attgttttta caagtaattc taaatcttgg aattctggag aattaacatt gaattcgtag
    19441 tcacgtctaa ctttacgctt actttcttga attaatttcc agtattgatc tctaatacca
    19501 aatttattcg atgtagtatc agctttacca atttcaactg ctttttgttt catctcatcc
    19561 caagaagcgt ctggttttgt agcaggttta acatgttcta atggagtttt atccattgca
    19621 aacaatgatt taatcgcttc tattttaatc aattgattct ctttaaaacc agaaatataa
    19681 ctatctttag tttctttagg gtttttagca taagatttca agtattcttt attgtgtaca
    19741 tgtggaaact gtaataaacg ttgtgcataa cgacgtttta attcagtaga catgtttttc
    19801 tgctggaaca tttgattaat ttgagtttga gacatacgag catcaaagtt ttgattcgtt
    19861 aaaccatggt ttgtaaacca ttgtggtgaa ataataaatg ttaatttctt accttttaat
    19921 ttatcatact gtgatgcaag ttcaactgcg ttaattaagt ctgtagaacc accagcacct
    19981 aataagaatg cttttttgtt ggcgttatgc ttatttaatg caattgcagg attaaatggg
    20041 tcatctttac ctaattcact agagccgtat ataggataat acttgtttga ttcgtataac
    20101 ttatcttgaa tgagtgtgcc ttttagtact tgatctgtca atgaagttct attatcttct
    20161 acagtctttt catttactaa tcctgtaaac caactagcag gtaataatag aaagacaatg
    20221 aataccgctc cactaattaa aatgggtaaa aaaggtttta atttcatcgt aactcttcta
    20281 atgcttcaac gattttattt ggtgttgccc actcatctct atcaaagtcc ataatagata
    20341 cttcgatatc aagtttattt tgaatctcta ataataatcc aactgtttgg aaagaatcaa
    20401 taataccttc ttcaaaaatt tctacgtctg gattttcttt tacaatatca ttttctgcta
    20461 cttctgctaa taaatttaat acttgttctc taaattccat aattaaattc tcctttatta
    20521 tataagttta cctgagaaga ttaaaaagcc aaatgttaca aagtggaatg taatcacaat
    20581 gctaagtgct gttgtgaaac cattttgcca acgtggcgga tgtttcttac gccaacgttc
    20641 ataatagcca taacctataa acaatgctgc atggtataaa ccataaacaa tgtaatacac
    20701 ttcgatacca tgccaaattc ccattatgaa gaagttgatt aagaatgcca cgttagacat
    20761 tgcaaattga ctcttcaata attttttacg agacatgtag aataaagatc tcatgtaaat
    20821 acaatctctg aaccagaatg ataatgtcat atgccatcta ttccagaaat ctttaatatt
    20881 tttcgctttg aaaggtttat cgaagtttgg tggtgtttta ataccgaata aataactaaa
    20941 tgctatcgca aataaactat aacctgcaaa gtcaaagaat aaatataagc tgtatgcgta
    21001 catatataac cacaaattga caaagccatg taagtctaat tgtaacggca tgattgcata
    21061 tgtgttaata aagtaagcaa caatatattt atacaagaaa ccaagcataa tcatgtgaat
    21121 tgcttttaat actaattcac gatattcatt gcctgttggt acttttttat cgtctttaac
    21181 gaaacgtttg taacgatcga ttggtccaga tgaaatcgtt gggaagaatg aaataaattg
    21241 aattaatttc catactttaa tttctttgat agaaccatca cgaatttcca taattaactg
    21301 cacacttttg aatgtaacat aagaaattcc taagaaacca acaaattcaa ttaatttact
    21361 ttcatggaag tgaatctgat gtccacctaa ccatgtactt tgtaacactt tcacaagtgc
    21421 taatggcaat attgataaaa ccattacagt tacaaatttt gaaaatgaat tattttttgg
    21481 ttttgaatga taataaaaca ttatcaataa aacttgccat acgacgtaaa taataaaact
    21541 aattaattga acacttaaat acttttggtc aaacagatta tgtttatcag aagaaaagat
    21601 taacacaatc ataaatgctg taactacgcc attataaatg taacttcgct tacctaaaaa
    21661 tccaagtata atgactggta ataatgcaat taaagcaatt aagaagaatg taaaatcacc
    21721 atatggaatc atccgttaat tacctctgca attttctttc tgtcaatttt accatttgaa
    21781 gtcaatggca attgttccat ccattcaaac tttctaggaa tcatatactc tggtaagcgt
    21841 gatttcaagt catttttaat atttttagtc atttctgcat tgtccgtaac ttcagtcgtt
    21901 ggcacaattg caccaattaa atgaataact ttatcatttt tatatacagg tacaacaatc
    21961 gcttctttta cgaactcaga ctggcgtaat tgtgtttcaa tttcttctaa ttccattctg
    22021 tagccattca atttgatttg gaaatcaata cgaccttgaa tgaaccattg accattttca
    22081 aacttcgctt tatcaccagt gtgatatgta cgaataccgt catcgaaatt aaatacttca
    22141 gctgtttttt ggtcattttt taagtatcct aaacttacac tttgaccttc gataacaagt
    22201 tcaccttcat ctgtagtaga taatcttgcg cctggtcttt caacgccaac aggtaatgtc
    22261 ggatattgat ctaagatttc ttgtgtaatt tgaatacttg taactgctac cgtagcttca
    22321 gttggaccat atgtgttgta aatcgtcgca cttgggaaac ggtttactaa cgcttttgct
    22381 gctctgtgag gtagaatttc accacagaag aagaattcgt taagactacc atattgttct
    22441 tcattaagcg ttggtaataa taaacacatt tccataaatg atggtgttga tacccaaatg
    22501 ttaatcggtg ttgctgttag catttcattt aataatttag gtttattaat catgttttta
    22561 tctacaagat ttaatgtacc gcctgatgct aaacatggat aaatagccat tacagataaa
    22621 tcaaatgaaa atggcgcttg gttaagccat tgttgttcat ttcctgattt attaagttct
    22681 aacatccact cagtaaattg aactaaactt gcatattcaa tttgaacacc tttaggttcc
    22741 ccagtagaac cagatgtaaa gattgtgtat actgtgtcgt tatctttaat ctgactatca
    22801 aaaattactg ggtcttgaga tgttttaata tcttctattg taaatacttc gccttctaaa
    22861 ctttcaaatg attcatcagt cgtattaaat acaaactctg gttgaacctt gttaataatc
    22921 attttaatac ggtcttcagg aattgaagtg tctacaggta catatccaca tcctgcttta
    22981 atggcaccaa tcatcccaac aatcatatat ggtgacatgt gaccgaataa aatcatcggt
    23041 ttcttactac cttgtaatcg atgtgctaat ttactagact catccattaa ctgttgataa
    23101 gttaattcat cagttgtgtg tctaacagca atgctttgtg gatttgcatc cgcaaacgct
    23161 tgcagcttgt taataatatc tgtcatatta agtctccctc attagaactc attataaatg
    23221 aagttattgt gtgtgtcgcc actgccataa attaaatata aagtaataaa tattgccaaa
    23281 tacaatagtg ttaataaata tggtttgaat gcttcaacat atttattagg tggctgttta
    23341 cttttagatt tcatattgca cctcttaaag ttcttagtaa aaacgccttt ataaagaccg
    23401 ttcaatataa aatacgtttt aaaatttgtt ttttacaatt cattatatcg atattcataa
    23461 tgaaattcaa ttttaatttt atagattcaa catagtaatt ggttgtcatc actcaattat
    23521 tgtaaatgat acactttgtt gagaacatca ttcattttaa aggttattta aacaataaac
    23581 aattacagtc tatataacaa ttttgttata tacgtcaaaa tcaaataaac tcatcacatt
    23641 aatatgacga gtttataatg ttattgaatt atcatcagcg caaatatata cattcgcaag
    23701 tcaagcataa catatttaac aattgctttg cttgttttac caatgattaa aaaccatact
    23761 tattttcaat ttactggagt atgtggtacc tgatttgtca taaccgcttc tatattatta
    23821 atacataatt gaatcatatt gtcccgtgtt gttactgatg cgctaccaat gtgtggtgta
    23881 atcagaacat tatcacgtcc cattaatgga tgtgtatgat caatcggttc atttgctaat
    23941 acatctaaac cacatgctaa aatttcttta ttgtctagtg catcgattaa tgctgtttca
    24001 tctacaattt gtcctctacc gatattaata aaaattgcat catttttcat ttgttcaaat
    24061 gcttcagcat taaatttatg atgtgtttct tttgtaagtg gcgctgtaca gatgataaaa
    24121 tcactttctg ctaacaacgt ttcaaaagaa acatatgttg cattaaagtc cgcctctgca
    24181 tctttatgtc ttgattgatt atgataaaga atattagtat taaacccttg caaccttctt
    24241 gcaaaagctt taccaatatc tcccatacca tatattccaa tagttgaatt gaagacatct
    24301 ttacctgaca ataaataagg accccagctt tgccatgcat ctgcttctac atatttttca
    24361 gcttctacaa tacggcgtgc tatagcaagc attaatgtaa atcctaattc tgcagttgtt
    24421 tcagtaagta catttggtgt attcgtgaca accacgttat tcgctgttgc actttcaaca
    24481 tcgatgttgt catatcctac agccatattc gcaattactt ttaaatttgg tgattgcgct
    24541 aaaatttctg catcgatctg ttcacttaat gtaataaaac aagcatctgc gtcacgaagt
    24601 gctgtcacaa attgatcttt aggcataggc actaatgatt tttgccacat aacaacgtca
    24661 ccaagtttac ttaattgttg ataaaattta tctggaattt tcctcgaaac aactattttt
    24721 accaaatcaa ttcacctact ttataaaatg actatatcta tttttctaat tcagctattg
    24781 cttcatttaa atctttaaaa gaatacgttg gtggcacatt tttattttgc acatcttcta
    24841 acgtagatac acctgtttgt acatgaatcg tatccatacc tacgttaata ccagacataa
    24901 tatcggtatc gtacagatcg cctaccatag caacttcgga tttatctaat cctaaaattt
    24961 ctaatgcttt aaccataata atcggttctg gtttaccaat aaattgtggc gatacacctg
    25021 tcgatacact tacaacactt gtaatagcac cattaccagg taataaacca cgctctttag
    25081 gaattgatac atcaggattt gtagaaataa atgttgcacc atttcttaca cctaacgttg
    25141 caatcgcaag cttttcatat gtaacttgtt cgtcaagtcc aataactaca taatcaacat
    25201 gctcgtcatt tttaatgaca agtcccgctt cggttaacgc agtatttaaa ccactcccac
    25261 ctaacatata tactgatgct cctggtgatt gttctgaaat ataatcagca gtggctaacg
    25321 ctgacgttac aacctcttct ggtttagcat caatgtgcat ttcacgtaat ttttcagtta
    25381 cttgctcagg tgtttttgtt gaattattag ttacgtataa atgaggaatg ccttttacat
    25441 ttaaataatc gatgaattgt tttgctccat caatctcatc tgttcccata tacattgtgc
    25501 catctaaatc gattaaatac gctttatact gtttcattat tgattttctc ctttaccaaa
    25561 agctgttact ggtacatttt cttgatgtaa aaatgtcgtt acttctgtaa taaattgttt
    25621 gtaataaggt aatgcgtcat caaacattgg catgataatc ttaatatcta aggctgtgta
    25681 atcatatgta aattgtttac gaataccgac agttttatta atcgcctgct gtgtttcttt
    25741 agtaataaca ttttctagtt ctaatatatc aatcacatct ttataattac caggatccct
    25801 taaaataaat gcatcgataa tcatattccc tatatctacc gatgattcta tcaacatttg
    25861 agcaatgcgt tcaaatgcat aatgattgtg cttgctctca tgataatcat cagttaatgc
    25921 ttgtaaatag gctaatttct gagttagttt atctttgtct acaaaataca ttccttgtca
    25981 cctctcactt atatattatc ataattttgc agtatacttc atttgtcgtt atactaactt
    26041 tactactttt aaaaggagtc gctacaaaat gattgatatg tatttatatg atgacaacga
    26101 agagagtcaa gttcagtttg ttggttttgt tggcgagcat agtcgatatg atttgatgtt
    26161 agtccataca aatagacatt atggtaagac actcgtactt aatatgcaaa caaataaatt
    26221 cggtattatc ggtactgatg atttaaaaga agaaggctat atcgctcata ttttaggtgt
    26281 aaatgctgaa gaaggcgatg aaattactga gtatttaaat gaagtcattc attaaagata
    26341 aaaaccccta actagattac aggtccatgc ttgaattgca tgattcccat tttcaatcta
    26401 gctaagggtt ttattaatgt tcagtatctt gaatggttgg cttaatcttc acatcttgaa
    26461 gtttattgtc gtcatctatc acatcagacg gatgaacttc ttcgattaac tttttaacct
    26521 cttgtttaga caaatgtctt aatacaaagt aaggacaacc aaagttacaa tattcaaata
    26581 tgtaatcttg aatatttgaa aaacgtttac tcatctctgc ttttttatta gaatctttgt
    26641 aaaaaccttt taatcgtaat tgatcgtaac catagtcacc aactatgtaa tcatatttat
    26701 ctaaaatatc tgaataccta gcaataaatt gttcttcatt aaaacattcg cgataatttt
    26761 ctattaattc aaagtaatgt tgatctactt ttatcaaatt cttcacctat ctatgacaat
    26821 gaaaggctca gtttatactt ttagtgtttc ataaaaataa tgattacttc tatgacacgt
    26881 tcgttttgta caaactaagc ctttatgcct tattaatggt taaatattaa ctatttaact
    26941 gtgcctcacc ttggcgttgc ttttctttag cagcttcatt tacttgctca tccgcatgat
    27001 aagaactacg tactaaaggt ccagcttggc aatgtttaaa ccctttatcc attgccactt
    27061 ttcttaattt accaaactct aaaggcgtgt aatatttttg aacctttaaa tgtttacgtg
    27121 aaggttgtaa atattgacca atcgttaaaa tatctacatc attcgcacgt aaatcatcca
    27181 tcgtttcata aatttcttct atagtttcac ctaatccaac cataatactt gatttagttg
    27241 ggatatccgg ttgtaattct tttgaacgac gtaaaaactc taatgttctg tcgtaagtcg
    27301 cacgcgcacg aactctcggt gttaagcgac gaacagtttc aatattatgg tttaaaatgt
    27361 caggtcttga cgccattaat gtttctaacg catcatagtc cccgcccata tctgatggta
    27421 aaatttcaat cgttgtaaat ggatttcttt ctctaacttt acgtactgtc tcagcataaa
    27481 catttgaacc agcatctctt aaatcatcac gcgcaacagc agtgataaca acgtgtttca
    27541 aattcattaa ttcaactgat tcagctacac gttcaggctc atttaaatca agttcattag
    27601 gtaaacctgt cttaaccgca caaaaacgac aagctcttgt acatacggca cctaaaatca
    27661 taaatgtcgc tgtacgacgt gcaccccaac attcatgtat attaggacat ttagcttctt
    27721 cacatacagt attaagattt ttttccctca tcatcttctt aagtcctgta tagttttcgt
    27781 tggtatttaa ttttattttc aaccaatccg gtttacgtaa tatttcctcg ttttttgtcg
    27841 ccataacaac acataccctc ctgttaagtg tatctttaaa cattatatcg aaattcatga
    27901 caaatgaaaa tccaataatc ttacttttta atattccaat aatttttctt taaatatatc
    27961 tcttaaaaac tctggcatta aatactcttt tggtaattct ttcaatgttg ggaaataacg
    28021 accgaacgta tacatatcta tcgtacctag tacatattct ttgtcgtctt cgatttcttc
    28081 tccattcaga tagtaacgcc ctgtagaatg aatgtaccct aaattataaa gaatatatcc
    28141 tccaaatata ttccctctga aacccaaacc ttgtgcatgt tcatacatat attcttgttt
    28201 attactttta gctataatct cttttaattt cacaccaaaa agtctaaccc ttaccatatt
    28261 aatcggatgc ggtaacattt gatgaatgtc atattctgtc acttcatctt ttacaatatc
    28321 tttaacgagt aagccagcat tgatgatggc acattgtgca tgtgtatact cacaaacact
    28381 ttgagctaat aagtatgcag cttcagttat gtgattcata ctacgcttaa gcactactgg
    28441 atgttgaatt actgaattgg acattaacgt ttttccttct tcttcaaatg aagtttcaac
    28501 ttcaggtaat gtttttaaag gaataatctt tgcagtttta tgtactactt tatgtgcctc
    28561 aaaagttaaa ttaacctctc caagataatt accatacttt ccagctgccg ccatcagtac
    28621 accattattg atttcaccat gttcaaaata atgatgcgta tgactaccaa aaatgacatc
    28681 aatttcaggc aattcttggc ataatgtttc atcgaagaaa atgccacaat gacttagcac
    28741 gattaataca tcaaatttac ctcgttgaag ttcaatttct tcttttatag attcaagtgg
    28801 atcggtaaca atccaattta gtgcacgata aaatggggta aaaggtgctg tcgctgcaac
    28861 gaatagtatt ttcacaccgt ctatgtcctt aatataagaa gaaacgatat tatttggtaa
    28921 atgacctgat tcatctataa cattgctaca tgtcactata aattttgctt cgtcgtaaag
    28981 gtgatttaaa gcttcgtgtg aaatggtcat cccttcatta ttaccgattg ttgcaacatc
    29041 acattttgct tcatttagta atgccacatt ctttttacct aaagttgctt cagttatagg
    29101 tgcggataaa tctacatgat cacctagatc aacatataaa gaaggatgat taagtcgtgg
    29161 ccgatgttct gccatatatg ctttaatgcg ttcgtattca tgtaaatgac tatgaatatc
    29221 gttcgtatga taaattgtaa gcctcaaatt gacgtcctcc ttttaaagtt gtattaaatc
    29281 aacgcacgaa taattaaata tatacccatt aacaacatta ctgttctcaa taatgttact
    29341 accgtatctg acttaattga ttgattcact ttgacaccga ttttcgcacc aaaataacta
    29401 gaaataatta aaatgattgc ataaccccaa gctacgtgac cttgagcaat gtgccctata
    29461 gaactcatga cacttgaaaa gaaaatcatc atcatacttg ttccaacagc tacatgaggt
    29521 ggaaatctaa atacaataag cattagtggc gtcattagtg cgccacctcc aataccaaat
    29581 aaacctgtca atataccaat taaaaacgtt gtaataaaag caaacaatgg tggaacacta
    29641 taatgatatg ttttaccttt agcgtctaca taagtctttt catacttggg tttatcgaaa
    29701 attttaaacg gtttaatctt atttcttacc attaataaaa tggctacgaa aattaaaaag
    29761 ataccaaaat ataaattaaa tgactcaaat gttaaatatc tactaatgaa ggacccaagc
    29821 aatgaacctg gtaatagtcc aaataaaaag atggaaccat ttttaatatc aacttgtttt
    29881 gtttttaaat atccaagtga tgaagaaagt cctgtcacaa ttagaatgac tgaagacgtc
    29941 cctatcgcta cttgtgttgt aatattatgt agtaatccat gttcaacacc gaggtaaacc
    30001 attgttggaa cgataataat accgcctcca atgcctacga tagaccctat aatcgctgac
    30061 aaacctccga ttaaaactaa taatgtaatt gttaataaca tagtcgtcgc ctcttaaaat
    30121 aattttaatt gttgtggtgc taacccttca tattctatat tcaataatcg ctgatatgtt
    30181 ttggcattat ttgctgcatg accaccagaa ttattgttaa atatgacgta aactttctta
    30241 gctttttgtg ctaatatttg tgccttttgt gccaagtcta ttaattcttg ctcattataa
    30301 tcatataaat agcgtacatc gcgccattct tgatctgaca tatctttctt agtccaaccg
    30361 taatgattac gtccatgata acgtacaaac gcaatttcat ttgtgattcg attgactaaa
    30421 ggtacactgc catctttcac ttgtggttca tcaactactg catgaatgat ttgatgttct
    30481 gttaaaaatg ccaatgtttg ttctttaaat gcatcactaa accatgattg atgcctaaat
    30541 tctacacaca ttggaaatgc ttgtaattgc tgtcttacat acaaaatata tttgatattt
    30601 tgtgcattgc agtcaaacca tggcggaaat tgaaccaata ccattgctaa ttttttctgt
    30661 gtatgtaagg gctctaacat attcttaaat tgatcaaata gttcttgcct tgtatctgca
    30721 aatgttttgt agtctgcatg caatgtgagt gcttgatgaa ttttgaccac aaattcaaat
    30781 gtatcaggcg tttcttttat ccatttcaat atatttcttt ccggttgtat cgcatagtat
    30841 gtcgcatcta attcgacaac cggaaaatgt ccagcatatg ttttaagttt atcggtttgg
    30901 cgttctaaat cttcatataa tgaatagtga tcaccccaac ctgttaatcc gatgtttatc
    30961 atatatatca ccaatgtcat cataccatat aacttttatc ataatcattt cagcgaactt
    31021 tagtttgata atttcgtttg actagattgg taaaatagaa acgcaatcca caaaaatggc
    31081 aagcactaaa ataatgtttg ggggtgcttg tgcttttgtg gattgcggtc gattcgatta
    31141 tttatattgc atgatttgat taatttgatt gattatattg gacatgatgg tgttggcggg
    31201 atgcgttgtt gctagtcgcg ggctttgtcc actccacata tgtgttaact ctttgtcgcc
    31261 gatgtttgcc gcggcttttc ttatgctact tgttagctca ttttgtattg gataatctgg
    31321 gatgtcgcct tcgtattggg acatttctta gataaaccta ttgttgatac cgcgtgcaag
    31381 ctttccacta aacgcttttg taacgactgt atctgtttct ttactattta taattgcgtc
    31441 tcgtagtagt tctgatgcat tactatcttg tgatgttaaa aatgcagtgc ccatttgtac
    31501 cccttctgca cctaagacaa tacttgccaa aactcctcta ccgtccataa ttccaccagc
    31561 ggcaatgacc ggaattgaaa cgacatctac aatttgtggc actaaagata ttgttccaac
    31621 cataggtaat tgatttttag gttttaaaaa tgaaccacga tgtccacctg cttcactacc
    31681 ttgagcaacg atagcatcca tacccgcttt ttcattcgca atagcttcat caacacttgt
    31741 tgctgtacct attagcttaa tgtttgcttc tttcaatctt tttataatag attcgcttgg
    31801 aattccaaaa gtaaaacaac atacaggcac ttgcttttta attatcgtat caatatgaca
    31861 cttaaattgt tgttcttcgg taatttttac aaccggctct tctaaatgta atgcgcgtcg
    31921 ataaggtttt aaccatgcat tcatattttc aatttgacta ctagtatatg attgttgact
    31981 tggtacaaag acatttacgc caaaagaatt tgacgttaat tggcgtacat aatctatttc
    32041 atcttccaat tgctgcgtat taaagtaacc tgcgcctatt gtgcctaacc caccactgtt
    32101 acttactgat gcaactaatt tcggtgtcgt acttcctgcc atacctgctt gtataattgg
    32161 atattcaata cttaacattt gagtaagtcg attcttattc cacatagctg ttcgctcctt
    32221 atatagatac gttgcgattt ttccgttgtt gaaattgaat ttgctgttga gaaagttttt
    32281 ctttttcctt tttatccatc tcatcttcaa tttccatacc taataattct tcaattaagt
    32341 cttcatgtga cactatcgct tcagtaccac caaattcgtc caacacaatt gctaaatgtt
    32401 ttctagaaat agtcatctta cgtaataccc attcagcttt attgtgttca ttcacaaata
    32461 atggcttagc tgaatagttt gtaatttgat tttctttttt attactccaa gccaacagat
    32521 atttagaatg aaacactcca ataatgttat caatatctcc ctcgtacact ggatatctag
    32581 tgtatggctt attcataacc gtttcataaa cttcttcgta tgtcgcattt gaagcaaatg
    32641 ccgtcacatt aattctaggt gttgtatcta catcttttac ttttaaattt tcaaaattaa
    32701 tgacaccttc caacctactc gtctcaattt catttaaagc accttcatgt ccagcaattg
    32761 ctaacattgt tttaaattct tcttttgaaa attgatgttc ttgaggttga cccttagata
    32821 aacttcgatt aatactgtcc gtcaacttat ttaaaagtaa tgtgataggg cgaaacacaa
    32881 tgacacaaat attaataatt ggatatacaa gccttgttat tttatctgga aatgttgcag
    32941 cgacagactt gggaatcact tcggaaatca aaatgataac aactgttaaa acagctgatg
    33001 caataccaac gctaatcccc caacgtaaag ccataattgt aacaagtgtt ggtaataaaa
    33061 tattcgcgac attattccca attagaatcg ttgtaataaa ctcacttggt ttttcaagta
    33121 actttacaat gccttttgct tttttatcac ctttgtcagc ttcagtttta aattttgttt
    33181 tattggcagc cgttaatgcc gtctcgcttc ctgaaaagaa aaacgaaata aatatcaata
    33241 taattatggc aatgatcacg cgtgtagtct ccttattgtc atatcttatt tttat
//
LOCUS       DBNENW010000012        31419 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNENW010000012 DBNENW010000000
VERSION     DBNENW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.012"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            177..623
                     /locus_tag="AC1LW8_000450"
     CDS             177..623
                     /locus_tag="AC1LW8_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4772316.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTQLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            839..1267
                     /locus_tag="AC1LW8_000451"
     CDS             839..1267
                     /locus_tag="AC1LW8_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIP4772317.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1374..2543)
                     /locus_tag="AC1LW8_000452"
     CDS             complement(1374..2543)
                     /locus_tag="AC1LW8_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HIP4772318.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2912..3827
                     /locus_tag="AC1LW8_000453"
                     /pseudo
     CDS             2912..3827
                     /locus_tag="AC1LW8_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
     gene            3806..4546
                     /locus_tag="AC1LW8_000454"
     CDS             3806..4546
                     /locus_tag="AC1LW8_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP4772319.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQVSKLDKKISDLQLIRRSV
                     CEFINGHSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4804..5457)
                     /gene="nreC"
                     /locus_tag="AC1LW8_000455"
     CDS             complement(4804..5457)
                     /gene="nreC"
                     /locus_tag="AC1LW8_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HIP4772320.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5480..6514)
                     /gene="nreB"
                     /locus_tag="AC1LW8_000456"
     CDS             complement(5480..6514)
                     /gene="nreB"
                     /locus_tag="AC1LW8_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HIP4772321.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6538..6990)
                     /gene="nreA"
                     /locus_tag="AC1LW8_000457"
     CDS             complement(6538..6990)
                     /gene="nreA"
                     /locus_tag="AC1LW8_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HIP4772322.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(7010..7687)
                     /gene="narI"
                     /locus_tag="AC1LW8_000458"
     CDS             complement(7010..7687)
                     /gene="narI"
                     /locus_tag="AC1LW8_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIP4772323.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7680..8255)
                     /gene="narJ"
                     /locus_tag="AC1LW8_000459"
     CDS             complement(7680..8255)
                     /gene="narJ"
                     /locus_tag="AC1LW8_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HIP4772324.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISTDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8248..9807)
                     /gene="narH"
                     /locus_tag="AC1LW8_000460"
     CDS             complement(8248..9807)
                     /gene="narH"
                     /locus_tag="AC1LW8_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HIP4772325.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9797..13486)
                     /locus_tag="AC1LW8_000461"
     CDS             complement(9797..13486)
                     /locus_tag="AC1LW8_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HIP4772326.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQGMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNENEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13793..14770)
                     /gene="cobA"
                     /locus_tag="AC1LW8_000462"
     CDS             complement(13793..14770)
                     /gene="cobA"
                     /locus_tag="AC1LW8_000462"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIP4772327.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGYKHDALMLCEHLYDEG
                     YGCLLNPNNTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14761..15075)
                     /gene="nirD"
                     /locus_tag="AC1LW8_000463"
     CDS             complement(14761..15075)
                     /gene="nirD"
                     /locus_tag="AC1LW8_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HIP4772328.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKDKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15079..17484)
                     /gene="nirB"
                     /locus_tag="AC1LW8_000464"
     CDS             complement(15079..17484)
                     /gene="nirB"
                     /locus_tag="AC1LW8_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HIP4772329.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17550..18281)
                     /locus_tag="AC1LW8_000465"
     CDS             complement(17550..18281)
                     /locus_tag="AC1LW8_000465"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIP4772330.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGEEQQVGVMVVAHGNINGKFTKAHEELQICTQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18510..19037)
                     /locus_tag="AC1LW8_000466"
     CDS             complement(18510..19037)
                     /locus_tag="AC1LW8_000466"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4772331.1"
                     /translation="MMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDAVKE
                     NIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQLLT
                     YVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMRKVL
                     IPERFDETILGQPPF"
     gene            complement(19281..20105)
                     /locus_tag="AC1LW8_000467"
     CDS             complement(19281..20105)
                     /locus_tag="AC1LW8_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIP4772332.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20322..20504)
                     /locus_tag="AC1LW8_000468"
     CDS             complement(20322..20504)
                     /locus_tag="AC1LW8_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772333.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20588..21055)
                     /locus_tag="AC1LW8_000469"
     CDS             complement(20588..21055)
                     /locus_tag="AC1LW8_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIP4772334.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21241..22788)
                     /gene="adcA"
                     /locus_tag="AC1LW8_000470"
     CDS             complement(21241..22788)
                     /gene="adcA"
                     /locus_tag="AC1LW8_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HIP4772335.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(22976..23047)
                     /locus_tag="AC1LW8_000471"
     CDS             complement(22976..23047)
                     /locus_tag="AC1LW8_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772336.1"
                     /translation="MLGQYEKAISISNMIEIVFFLIL"
     gene            complement(23082..23348)
                     /locus_tag="AC1LW8_000472"
     CDS             complement(23082..23348)
                     /locus_tag="AC1LW8_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIP4772337.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23348..23599)
                     /locus_tag="AC1LW8_000473"
     CDS             complement(23348..23599)
                     /locus_tag="AC1LW8_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIP4772338.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIVDLERGKTITKDIDV"
     gene            complement(23764..23844)
                     /locus_tag="AC1LW8_000474"
     CDS             complement(23764..23844)
                     /locus_tag="AC1LW8_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772339.1"
                     /translation="MNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(23872..24471)
                     /locus_tag="AC1LW8_000475"
     CDS             complement(23872..24471)
                     /locus_tag="AC1LW8_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIP4772340.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24490..24852)
                     /locus_tag="AC1LW8_000476"
     CDS             complement(24490..24852)
                     /locus_tag="AC1LW8_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HIP4772341.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            25107..26357
                     /gene="fmhA"
                     /locus_tag="AC1LW8_000477"
     CDS             25107..26357
                     /gene="fmhA"
                     /locus_tag="AC1LW8_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HIP4772342.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26454..27185)
                     /locus_tag="AC1LW8_000478"
     CDS             complement(26454..27185)
                     /locus_tag="AC1LW8_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP4772343.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     EAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(27182..27901)
                     /locus_tag="AC1LW8_000479"
     CDS             complement(27182..27901)
                     /locus_tag="AC1LW8_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP4772344.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27882..28661)
                     /locus_tag="AC1LW8_000480"
     CDS             complement(27882..28661)
                     /locus_tag="AC1LW8_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP4772345.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28782..30221)
                     /gene="mdeA"
                     /locus_tag="AC1LW8_000481"
     CDS             complement(28782..30221)
                     /gene="mdeA"
                     /locus_tag="AC1LW8_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HIP4772346.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLLSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSRQ"
     gene            30395..30499
                     /locus_tag="AC1LW8_000482"
     CDS             30395..30499
                     /locus_tag="AC1LW8_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HIP4772347.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30653..31339)
                     /locus_tag="AC1LW8_000483"
     CDS             complement(30653..31339)
                     /locus_tag="AC1LW8_000483"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIP4772348.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
BASE COUNT         9983 a         6046 c         4460 g        10930 t
ORIGIN      
        1 tcaatttaaa aaatcagtca tatcagtaat taccgatgaa aatactaaga tatcgatata
       61 ttttgcatta tttaacattt aaattcaatt atagacaatt caataattgc gatgaaatca
      121 gtacttgaca actattacac ttcataatat tgtttatttt aacaaggagt gattggatgt
      181 atgtagaaaa cagctatctt agcaaacagt tgtgtttttt attttatgtt tcttcaaaag
      241 aaataattaa aaaatacaca aactatctta aggaatatga tttaacctat actggttaca
      301 ttgttttaat ggcgattgaa aatgatgaaa aacttaacat caaaaaatta ggtgaacgtg
      361 tattcttaga ttctggaaca ctgacacaat tactaaagaa attagaaaag aaagattacg
      421 ttgttcgaac acgtgaagag aaagatgaaa gaaacctaca aatttctcta acagaacaag
      481 gtaaagcaat aaaaagccct cttgctgaaa tttctgttaa ggtctttaat gaattcaaca
      541 tttcagaacg agaagcatca gatataatta ataatttgcg gaattttgtt tctaaaaatt
      601 ttgactattc cgacaaaaaa taactcccta tcataaggtg atgggcagaa gtatgcatcg
      661 ctatgtttat tcattatctt tattgcttgt ataaatctcg atgctcagtt cttttgccct
      721 gattccttat caggcacaaa tctacccatt tattactttt ttatctcccc ttttacttta
      781 acttttttaa tactactcat ataatggctt tgtaatacaa atttctataa tggaagtgat
      841 gatattgaac ttcaatcaat tcgagaatca aaactttttt aacggtaatc caagtgatac
      901 atttaaagat ttaggtaaac aagtatttaa ttacttttca acaccttcat ttgtaacgaa
      961 tatatatgaa acagacgaat tatattactt agaagctgaa ctagcaggtg taaataaaga
     1021 agatattagt atcgatttca ataataatac gctcactatt caagctacta gaagcgcaaa
     1081 atacaaatct gaacaactca ttttagatga gcgtaacttc gaatcattaa tgcgtcaatt
     1141 tgattttgaa gctgttgata agcaacatat tactgctagt tttgaaaatg ggttattaac
     1201 cattaccttg cctaaaatca aaccaagcaa tgaaactact tcatcaacat ctattccaat
     1261 ttcatagtta aatttaaaat gaatacacta ctaaagcctt aatgtcatgc tacgaatatt
     1321 gcgcatgata ttaaggcttt gtatttaact aaatgaatgt catgtattac atatcaaccg
     1381 tttttcaatg agccatattc tttttggtat aaatgacgca tggtaaataa tgcaatacat
     1441 ccaaataccg ctaagaaaat aaatgctaaa tgacttgatc ctgttaaatt agctacgtac
     1501 gtgattacta gtggtgggaa gaatcctcct aaaccaccca tcattgatac gataccattt
     1561 gcggatcccg cttcatttaa gaagtaggat ggtactaatt tgaagattaa gccgttacca
     1621 atacctgcac aaatacttat tgttaaacag cctacagtga ataatgcgat atggtctgaa
     1681 atacctaaaa taatggcacc gataatcata acaacaaaat caatcatcaa tactttaact
     1741 gcattaaatt tatcacctaa aatgccacct attggtctta agaatgttgc cagcgcaatg
     1801 aatacacctg atcgaatacc agcatctact ttgtcaattc caaaatgatt tactaagtag
     1861 ttaggtaaga aaataccaaa tgctacaaaa gcaccaaaag taatgaaata ccaataactt
     1921 aagtaatata atttgtagtt tttagataat gttttcattt gtgccattaa tggcacttta
     1981 attttacgtt cttgtgtatc accaaatata aacataatta atgcaaataa agcgataata
     2041 attaagtaac ttctaacagt tgtttgccaa ccaataatac ccgcaattgg cggtgctaaa
     2101 aatgaagaaa ctgctgtacc gatatttccc ataccataaa taccatttgc tagacctact
     2161 ttttctttag ggaaatattt aggaactgat gtaacaccaa ctgagaaaat tgcaccacct
     2221 acaccaagga aaaatcctga cgccattaac atacccggtg tttgtgcttg gcttaagaaa
     2281 aatatcggga ataacaatac gataaaacta gtaaagaata cccatttagc gccaacaatg
     2341 tttgttagat aaccaaatgg cacacggagc accgatccca aaataactgg tatcgctaaa
     2401 atgattgata tttgaccttc agtaacattg acatcttgtt taataaaggg cattaatggc
     2461 gcaattatac tccaagccat aaacccaacc actaaactta atgtctgtaa tgtaagttga
     2521 aagccacctt ttgttttgta catacgtttc accttcacca aaatcattta ttttttaata
     2581 gatatcaata ctctgcctat atattagagg tcaatgttat ttaaaaatgc tttcatttat
     2641 atgaaatcat tcaatataga caaagtattg actacatata tattttatta tgattgtgaa
     2701 aaagtgaaca taaggatttt cccttattta tgaagtgatt taccctatac aatgtccctt
     2761 taagatgctt ttagacactt attcgccatc attttaatca tattatttag gaacactcac
     2821 atacttttct tatttttttc aattagttac aattactgct tgaaaatcac gttacgttat
     2881 cctttatgct gacaacataa ggaggtttat catgtataaa atcaatatta ttagatcaga
     2941 ttccgtctat aagaatatac tcaaggcacc aataaacgac cgtgattcaa ttttcactaa
     3001 agaaatatta gtacctttta aaaagaaatt tgaggtacaa catatgccta tttataatga
     3061 tgacaaacaa acaatgagtg tgattcaatt tttagatgca tttcaaaagt ctcccaaaga
     3121 actggttgaa actgaccaaa tgtcaataca atacctgaat aatgatttct ggagtaattg
     3181 cgaaaaatat ttaaaagttg ctatagatca attttcaaat tatagtatct catttcaagt
     3241 ttctaactat catttcacag tattacttgg agatagccaa aaaccactta tgtatctaaa
     3301 taaaaatcgc ggtggtgatg gtggcatacc aggttatatt atgatttatt tagtgccgag
     3361 tacaagtaca attaattcta tgaaaagctt aattgcacat gaagtaaatc ataatatgcg
     3421 ctatcaatat tattgattgg gatggcggaa gtttgattga attgattatt gcagaaggat
     3481 tagctgaaaa ctatgtagaa tcattgtatg gtaaagcgca tattggaccg tgggtaacaa
     3541 atactaattg gagtcgtgat aacgtaaaaa ttaaaaatac tatttataat catttacatt
     3601 taaagcatat atttgaatcg atgccttatc tctatggtga tgatattaat aaacttcaag
     3661 gtaggcctat cgttggctta tctcatgctg ccgggtatgc atgtggctat cacttggtaa
     3721 aatacttttt acaaaaaaca aacataccta ttgaagttgc cacaacactt ccagcacaaa
     3781 aaataataaa tgaggtgact gaattttggc atacacatac actttaaaag atattattga
     3841 aattacaggt gtaactaaaa gaactttaca ttattacgat gaaataggat tattagttcc
     3901 agataaaaat gataaaaatt atcgcgttta taaacagcaa gacttagaaa aattacaaaa
     3961 gattttaata ctcaagtctt ttgattttga tatcgctaaa ataaaacaat acatttcata
     4021 tgataatgaa caattgcgaa agttattatc agagcaagta agcaagttag ataaaaagat
     4081 ttcagactta caattaatta gacgctctgt atgtgaattt attaacggac actctctaat
     4141 agataccagc attttaaata agacactaca gtcacaatat gataaagaag catctataaa
     4201 atatggtcat acgaaagcat atcaatcatt tattagacgt aaagacagct tacaatcgca
     4261 ggatatcaga cataaattga caactatctt caataaattt aatcatatgt ctttgagtca
     4321 ttatccaatc caagattgta gtgatctcgt atttgagtgg aaggcattta tgaacactat
     4381 cgctgatttt gatgatgaaa cattatgctg tattgctaaa acatatgaag atgatacgcg
     4441 tttcaaagat tactttaatt catatgataa tcaaaattta gcatcataca tttcagaagc
     4501 tgttaattat tttttgagca atgtgaataa gagcgacaat ttttaatcct catacagatg
     4561 ctacaacact gaatacacca actaaaataa ttgttcaaag tagaaaacta atcatttatt
     4621 ctctaaatca ataactatat taaaagttat acctttgcaa agcgaattag taaaggttac
     4681 cgaaaggaga aaggattagg ttccattcga tttataaaaa aatatatttc gaaacataca
     4741 aaagcgccag aagataatca tttcctctcg cgcttttata ctgaattata ttaatttcaa
     4801 actctaaaac tctaataatt tctttttcaa tgcatattca actagctcag gtttactctt
     4861 taagccaagc tttgtcataa tatgcgtctt atgtgcttct actgttttca cagatacaaa
     4921 taatttctct gcaatttctt tattcccgta acctttggca ataagtggca atatttctag
     4981 ttctcgtttt gataagattt taaaaggatc tgttgtgttt gcagtgtcct gatttgaatt
     5041 agatacaaat tcattcacta aagatgttgt aagtttcata tctacatatg tttcaccttt
     5101 atatacagtt cgaatagcta acaataattg ttcatcaggt gcatttttca atatgtaacc
     5161 tttcgcacca ttacgcaaca catggaacaa atactcctca tcatcaaaca tggttaatat
     5221 taaaatttta gtttctggaa aactgtcagc aattttactc gtagcgataa gacctgactc
     5281 acctggtggc atacttaaat ccattagtaa cacatcaggt ttatattcca ttactttttg
     5341 gtaagcttcg acgccatctg cagccgttgc aacaacttcc atatcatttt gataatttaa
     5401 aatcatagag aaccccgtac ggacaacagc gtgatcatcg gcaatgacta ttttcaattt
     5461 tattccccca atgtatgttt caaattggaa tgttcaatgt aacattggta ccctcaccaa
     5521 ttttcgtttc aatattgacg ctaccgctga ctaactcagc tcgctcattc attccatata
     5581 aaccgagtcc agaaccttta ggcttagaac ttggatcaaa accatttcct gcatctatca
     5641 cttctgctac caaatggcgc ccagtttgac ggatacctac atttatttca tttacatcag
     5701 cgtatttcaa cgcatttaga atagcttctt gtactactcg ataaacaacc gtttcaatat
     5761 cactatcaaa gcgggtattt ttaatatttg atgtatatat gatttttatt ccataatttt
     5821 cttcaaactg tttaaaatat gatttaaaag ctgcttcaag gcctagatca tccaaagaag
     5881 cgggtcttaa ttcaaccgac atattacgta tatcatcaat taatttagcg acaatatatt
     5941 caatattttc tgcgtcttcc aaaagcttag ttgtatcttc ttgatatttt aataatctca
     6001 attgaacatc tacattgagc atttcttgaa tcacactatc atgtaactct ctagaaattc
     6061 gctttctttc attttcttgg gctgagattg ttttacgcat catacgttgt tgatgtaatt
     6121 tctcttgctg ttcaatttgt gatgaaacat tttgaagcgt aaatgcatga attcccctgt
     6181 cttgatcaat caactgatat gttgctgtaa atggcatcac tttttgatct ttcgtcttca
     6241 taaatacttg gaaattcgta gcttgtactt gcatcgattc taagaaacag tctttacatg
     6301 attgtacatc ataggcattt gtataccctt cacatctgtg acaaatcgca ttcgccacag
     6361 cactgtaatt atcttcttca gataatatat ctttagcagc atcattcatt gcaataattt
     6421 tgccgttatc atcagcaaaa actatctttt cgattgaatg ctcataatat tttttcaata
     6481 aagtatctaa ctgtatactg tcctcattaa tcatgactta caccctaatt catctcatta
     6541 tttatcatca ttgaaaatac caaacttacg ttgaatatca tcattatcaa atatttttgg
     6601 taaaggacga ccatctcttt gaccaaataa tagtacgcca tacacttgat tcttatacca
     6661 aagcggcact gctaaaactg ctgttaatga ttcgctcaat aaaattggat agtcaatctt
     6721 ttcttcaggc cctaaagcta aaccaacatt ggctattacc atacgctttc ctgttttcat
     6781 aacagttcca gctaatccac gaccttttct taaaataatc aatttaaatc gattattttt
     6841 attacctgaa acatagtgcc attttattgg agatgatggt ttgttagatt catagaaagc
     6901 gattgccgca aaatcataac cctcttcttt gcgtatttta tctaatgtct cttgaaatct
     6961 acgatcttca attattgctt ctggtgtcaa atcctttcac ctcttatgct tacactttat
     7021 tcttacggta aataatatat ctgcgattta tatatgtcaa aggtacactc caaacatgca
     7081 ccaaacgtgt aaatggccaa caagccataa tagtgaaacc taacaatata tgcattttaa
     7141 atgcaatcgg cacaccactc atcaatgacg catctggttt taacataaat aattgtctaa
     7201 accaaattga taatgaagtt ctgtagttaa aatctggatg ttgtatattg gttactaatg
     7261 ttgcgtaaca tcccataaat acgataagta ataataagaa atttacaaat atatccgacg
     7321 ctgaacttaa tcttcgaata cttttcgtag taacacgtct cgctgttaat aaaaacatcc
     7381 ctatcaaagt tattatacca aagatgctac caatataaac agcgcctata tgatataaat
     7441 gctcagacac acccactgca tccatccatg atttcggtat taacaatcca actacgtgtc
     7501 caaaaaacac tggaataata cctaagtgaa ataataaact tccccacatc aatctttttc
     7561 tttctattag ttcactagat ttagctgtcc aagaaaattt atcataacga taacgtgcaa
     7621 tatgacctgc gacaaagaca actaaacata aatacggaaa tataacccat aaaaactgat
     7681 taagcatgat gtttcactcc ttttggtgat gtcaaacata atttcaatgt ttttctaagt
     7741 gcttgaatca cataggcata tggattgtta tcttcaccaa gtgcattcgc catcacatat
     7801 gttccatcct caataatcat aatgattaat tgaatattct cttcagctct tggatcattt
     7861 cgccattctg ccacttgcaa aaattgaagc atcaacggta gataatcaga aagttcatta
     7921 tctaccattt ctagtccaaa catttcatat aataccttta atttagctaa catttgccca
     7981 cgttcttttt gcgtatcaaa tttgttatac gtcatatata atggtgcttt tttcgtaaaa
     8041 tcaaatgtat ctgtataaat cgctttgatt tctgataatg aaaatgtcat cattacattt
     8101 ctaaatgcta ctaaatcttc atatgctgga tgatctgtcg aaattgtttc ttcaaaagtt
     8161 tttggatgaa aagttaattt ttctggaaag cataactgtt gtgccatata tccaaaactt
     8221 tcttgatatt ttttaaaatt atcgaaatta atcacggaaa atccctccat agaaattctc
     8281 attataaatt tcttgaccag ttttccctga acctactgca acgccacagc cttcacagtt
     8341 atctccaaaa tgctcgccgc cgtaattgta tcctgtacta ccttgtgcgt gatacgtatc
     8401 taaataggtt tctttgtgtg atgttggaat aacaaatcga tcttcatatt tggctagtcc
     8461 taataaacga tacatgtctt tagtttggcg ctcggttata cctaatcgct ctaatcgaga
     8521 cgtgtcaaat ggctgttgag taacttgaga tctcatataa cttctcatca ttgccatacg
     8581 ttgtagggct ccttttactg gctctgtatc tcctgcagtg aaaatattag ctaagtattc
     8641 aataggtaaa cgcatttctt caatggctgg gaaaatcgca tctggatttt gagttgtatt
     8701 tttaccttca aaatagctca taattgggct aagtggtggg caataccaaa ccatcggcat
     8761 cgttctaaat tcaggatgta acggaaatgc aagtttatat tcaattgcta acttataaat
     8821 tggagagttt tgtgcagctt caatccaatc ataaccaata ccatcttttt cagcttgagc
     8881 aatgacttct tcgtcaaatg ggtttaagaa tatatctaat tgtttttcgt ataaatcttt
     8941 ctcgtctact gctgaagccg cttcatgaac tcgatctgca tcatataata aaacacctaa
     9001 gtaacgcata cgtcctgtac aagtttcaga gcataccgta ggcatacccg cctcgattct
     9061 cgggaaacag aaagtacatt tttcagcttt gttcgttttc caattgaagt aaactttctt
     9121 atatggacaa cctgtcatac agtaacgcca tccacgacat gcgtcttggt caactaatac
     9181 aatgccatct tcatcacgtt tatacatagc acctgaagga cacgatgcaa cgcaacttgg
     9241 attcaagcaa tgttcacata aacgtggtaa atacatcata aaagtttcgt caaattggaa
     9301 tttaatatct tcttctattt tttggatgtt aggatctttt ggacctgtaa catgaccacc
     9361 tgctaagtca tcttcccagt taggtcccca ttcaatttca atgttatccc ctgtaatttc
     9421 tgaatacgct ctagcaactg gcgaatgctt ccctgatttc gcagttgtta aatgttcata
     9481 attatagttc catggctcat aataatcttt aattaatggc atatctgggt tataaaaaat
     9541 tttacctaaa gcaatttttg aaattctact tccagatttt aattcaagtt tccctttacg
     9601 atttagtacc caaccacctt tgtagtgttc ttggtcttcc caacgtttcg gataccctac
     9661 acctggcttc gtttctacgt tgttgaacca catgtactca gcacctggac gatttgtcca
     9721 agtgttttta catgtcacac tacacgtatg gcatcctatg catttatcta aatttaatac
     9781 catcgcaact tgcgctttaa tcttcaagcc aattaacctc cttcatcttt ctaactgcta
     9841 catataaatc cctttggttc ccaattggtc cataataatt aaagtgataa ctaatttgtg
     9901 cgtatcctcc gactagttgt gttggtttca aatggattct agtcggcgcg ttgtgtgaac
     9961 caccacgtgt atctgtaatt tctgacccag gcgtttgaat atgtttatct tgtgcatgat
    10021 acataaacat tgtaccttta ggcatacgat gcgaaataac tgctcttgcc gttacaacac
    10081 cattacggtt atacacttct agccaatcat tatcttggat atcgtgtttt tcagcatctt
    10141 catttgatat ccaaaccgtt ggaccacctc taaatagtgt caacatatgc ttattatctt
    10201 gatacattga gtgtatattc cattttccat gaggcgttaa ataacgcagt accaaagcat
    10261 ctgtaccacc tttaattttc ttatctctat tcccaaatac cattggcggc aatgtcggtt
    10321 tatatactgg taagctctct ccaaattgtt ggaaaacttc gtgatccaca taataacttt
    10381 gacgtcctgt taatgttcta aaaggtacta gacgttctat attcgttgta aatggtgaat
    10441 atcgtcgacc ttgtttattt gaacctggga atactgctgt cggtattact tctcgtggtt
    10501 gtgaagttat atttaaaaac gaaattttct cagcagcgcg ttcgctagaa atatctttta
    10561 acggcattcc agtttgttct tcgagatctt catatgattt ttgtgataat ttaccattcg
    10621 tagcagatga aatacttagt attgcatcag ctacattacg tgctgtatca atacgtggac
    10681 gattcgctct cacagaatca tcatttgtat cactccacgt acctaacata ctttttaatt
    10741 cttcatattg ttcactgaca ccgaaactta caccatgtgc tccaactttc cctttttcaa
    10801 gtacaggacc aagcgtgaca tatttgtcgt aaattttagt gtagtcgcgt tctacaattg
    10861 caaagttagg cattgtacgt ccaggtaccg cttcaatttc acccttcgac caatctttca
    10921 ctacgccgta tggtgttgaa atttcttgct ttgtatcatg actaagcgga gttgtcacaa
    10981 catctttaaa cgttccaggt aaatagtctt ttgccatttc tgaaaatgct tttgccaacg
    11041 ttttataaat atcccagtct gaacgcgatt cccataatgg atcaatagct ggattaaaag
    11101 gatgtacata tggatgcata tctgtagatg acaaatcatg cttctcatac caagtcgctg
    11161 ctggcaaaac aatgtcagaa tataaaggtg ttgctgtcat tctgaaatct aaagaaacga
    11221 ctaaatctaa tttccctgtt gtttcctcac gccacgtaat ttcttctggc ttttcatttt
    11281 catttggtgt agctaataac cctgattttg tgccaagtaa atgcttcata aagtattctt
    11341 gaccttttgc agaacttgag attagatttg agcgccatat aaacagtgat ttcggatgat
    11401 tctttttcaa atccggatct tctatcgcaa attgcgtttg ttttgactta acttcattta
    11461 tcgctcgttt taaaattgct tcatttgaat ctataccttc atctttagct tcttctgcaa
    11521 acaacaaact atttttatta aattgtggat atgatggtaa ccaaccaagt ctagctgcta
    11581 aaacattata atcagctgga tgttgatgct ttaactcctc tgttttagct aatggagatt
    11641 ttaaacgatc tacatttgac tcttcatatt tccattggtc tgttgcaaaa tagaaccaac
    11701 ttgttccgtt ttgcaaacgt ggtggtcctt gccagtcttt cgcaaatgcg acagtactcc
    11761 atccttcaat cggacgacat ttttcttgtc ccacatagtg agcccaaccg ccaccattca
    11821 caccttgaca gccacataac ataactaagt ttaagattga acgataaatc gtatctgagt
    11881 taaaccaatg gttaataccc gcacccatga taatcattga acgcccttca gtatcgatag
    11941 cgttttgcgc aaattctttc gctacttgaa tgacaacact ttgttttacg cctgaaatgg
    12001 cttcttgcca agcaggtgta tattttgatt ctgcatcgtc gtatcctttt gattctaatt
    12061 tatgatcaaa acgacgcacg ccatattgac ttgccattaa gtcaaaaatt gtagcaatac
    12121 ggactttgtc accatttgct aaagtgactt gtcgagttgg aattggacga ttgaatatcc
    12181 catctccatc actatcaaag tatgggaatt gaattgtttc taattcgtat ccaccttctg
    12241 ccattgataa tgtagggtta attttagaac catcttctgt ttctagtttt aagttccact
    12301 tcttaccttc ttcccaacgt tgacccattg tgccattagg tactactaaa ctatcgctga
    12361 ttgcatcatg aataactggc ttccattcgc cttgctctgt tgtttgacct aagtcactcg
    12421 ctcttaaaaa tcgacccgct ttatatccat tttcatcttc atccagcatg ataagaaacg
    12481 gcatatctgt atattgttta gcgtaattta taaagcgttc attaggttga ttaacataat
    12541 gttcttgtaa aataacatgc gtcattcctt gtgcaattgc agcatctgaa ccaggattcg
    12601 gtgctagcca gttatctgca aatttcacat tttctgcgta atctggtgct actgaaatga
    12661 cttttgtacc tttatagcgg acttcagtca taaaatgtgc atccggagta cgtgttaaag
    12721 gtacattaga cccccacata ataatgtatg atgcgttata ccagtcgctt gattcaggca
    12781 catctgtttg ctctccccaa atttgtggag aggcaggtgg taaatctgca taccagtcat
    12841 aaaaactaag catttcacca ccaagcaaat tgatgaatcg agcacctgct gcataactaa
    12901 tcattgacat cgctggaata ggtgtaaatc ctgcgattcg atctggacca tattttttta
    12961 ttgtatacag taattgtgct gcgattatct ctgtaacgtc tttccaattt gaacgcacgt
    13021 gccctcccat acctcgggct tgcttatatt gtttggcttt gtcttcattt tcaacaatag
    13081 acgcccatgc agcaacgcga ttaccattgt tttcttctaa tgcttcagtc cataaatccc
    13141 agagttttcc acgaatatat ggatatttga ttcgaagcgg actgtattca taccaagaga
    13201 atgacgcacc tcgtggacat cctctcggtt cgtattcagg catatccgga ccacaacttg
    13261 gatagtcagt ttgttgattt tcccaggtaa tcacaccatt tttcacaaat actttccaag
    13321 aacatgagcc tgtacagtta acaccatgtg ttgttcttac ttctttatcg tggctccaac
    13381 gttctctgta cattttttcc cattctctac ttttactttc taggatcgac caattcccat
    13441 taaatttttc tgttggctta aagaaattca atccaaattt tcccatattt atatcctcct
    13501 acgtataaaa atacgatgtg tagatgtcgt gtttttaaat actttaaaat gcccaagact
    13561 attgctttaa ttagattgta cattttttca caaacataaa atattaggga atcacctaat
    13621 tacttaagga atttccctat caataacggg atttcattga aatattacac aatcatgtat
    13681 ggtcgtgctt attgccaatc taaatcgttc aaatttggca caacgacaaa taaggcttca
    13741 acacgaatat attctctcgg ttgaaacctt acttattcat ttatttttta aattagtgac
    13801 ataacactgt attagcatct gcacgatcgg ttgaaatata tgttacattt tcttgctgct
    13861 taataaatgc atcatagtaa tcatattgcg acgaatgata tgtgccattc gatgtattat
    13921 ttgggtttag caaacagcca taaccttcgt catataaatg ttcacagagc ataagggcgt
    13981 catgtttata accacttact acataaaatt gctgagtcgg attagaagtt gttgagacat
    14041 tttcagtata accaacaact tccccaataa tacatatacc tggtttcgcc tcaattgaat
    14101 agtgttgcaa ttttgaaata atattactta aacgcccctt aacaacaaac tcgttaaaac
    14161 acgatgcttg aaagacaatc gctatcgggt aatcaatatc tgtgtattgt tgtatctgtg
    14221 tgataatttt ccctaaacgt tttaccccca tataaattgc taacgtgcca ccattcacta
    14281 aggaattgac atccacttca ttttcttctg aatctttaaa gtgacctgta gaaaatgtca
    14341 cacttttagc aactgtacgc attgtcaaac ctgtctgcat agtagcaact gctgcgctcg
    14401 ctgatgtcac ccctggtaca atttcaaacg caatatgatg ttcatttagt gtgtcgactt
    14461 cttcttgcac acgaccaaat atcgctggat cgccaccttt aagtctaaca accttgttat
    14521 atcgacgcgc tgcttccacg atacagtcat ttattttttc ttgctgaata tgttttgcat
    14581 acggcttttt accaacatcg ataatttcag tagtcaaatt cgcatattgt aaaattaacg
    14641 gattcactaa tcgatcatat agaatgacat ccgcttcacg tattaaacgc tcagcctttt
    14701 tagtcaaata attcggatta cctgggcccg cacctatcaa gtaaaccttg ccatattcct
    14761 ctacagacat atatatacgt tcccgtctgt aacttctacc tcataaacat ctacacaacc
    14821 ttcatcaggt tcttgaacaa tacctgtatt taaatcaatt ttttgatcgt ggagcgggca
    14881 aaatacatat tccccactca ctgtcccttc agacaatggt ccttgtttgt gtggacagat
    14941 attgtgaatc gcatgaattt tgccactttc tgttaaaaac aatcctacct ctttgtcttt
    15001 gacaataacc ttttttccaa ttaggggtgt taattcatct atagttgtca ctttaatttt
    15061 ttcttttgtt tccatgtatt acaccttctc cacttcaaaa attttacgtg cttgcgcatt
    15121 gctagttatt gcttcccaag gttcagcttc gactgctttt ttagcatcca taatgcgttc
    15181 aaatagttca ttttgtcttt ctgggtcaag taagacttct tttacatttt caaatccaag
    15241 tcttcttaac catggcgctg ttctttcagc atatatacct gtttcgcgat aatattgcat
    15301 caaagcacca cataatttga ttacttcatc ttctgtttct acagttgtta aaaattcagc
    15361 tttttcaact tctgtaccac cattaccacc gatatagatt tggaatccat tttcaactga
    15421 gataatacca aaatctttaa cacctgattc aacacaactt cttgggcagc ctgatacacc
    15481 cattttgaat ttatgaggtg tatcgatgta ttcaaatgtt ttttctaaac gaatgccaag
    15541 tcgtgtcgtg tattgcgtac caaatcgaca aaactcttta ccaacacaac ttttaactga
    15601 gcgagttttc ttaccataag ctgatgctga acgcatacct aggtcttccc atatatttgg
    15661 taattcttct tttttaactc catacaaacc aacacgttgc gaacctgtca ctttaactag
    15721 tggcacatga tatttcttag ccacttctcc tagacgaatc agttggtctg catctgtaac
    15781 acccccacgc atttgaggta taacagaaaa tgtaccatca ttttgaatat tcgcatggta
    15841 acgttcgtta gcaaatcttg attctctttc atcttcatga tcatgtggat aaaccatatt
    15901 caaataatag ttaatcgcag gtcgacattt tggacatcca cctttgtttt taaagtttaa
    15961 aacatgtcga acttctttag atgtttttaa acctttcgct cttatttgcg ttacaatttg
    16021 atcgcgtgtc aaatcagtgc aaccacatat accagcaggt tttgcggcaa caaagtcatc
    16081 tcctaaagtg tgctgcaata tttgagcaat ttgcggttta catttaccac atgaattccc
    16141 cgcttttgtt ttagccgtta cttcttcaac tgttgtaaag ccattttccg taatcgcatt
    16201 tactatagta cctttatcaa caccattaca accacaaatt gtttcatcat cagccatatc
    16261 agcaattgat agcgatgcct cttctccacc tttagtaagc aatgatacaa gtgtgtaatc
    16321 ttcagtggat tcaccttttt tcatcatgtt ataaaagcgt gaaccatcat cgatatcacc
    16381 atatagtact gcaccaacta cattaccgtc ttttaaaaag atttttttat agttattatc
    16441 aacactatta aatatttcaa tacctttaat ttctgcattt tctacaattt gaccagcact
    16501 atacaagtca cacccagaaa cttttaatga cgtaaatgtt gttgatccct tgtatccgtt
    16561 cgtttcttta tttgttaaat gatcagctaa tactttacct tgttcatata gtggtgcaac
    16621 gagtccataa actttgccgt tatgttctgc acattcacca actgcatata cattgctatc
    16681 acttgtttgc atcacatcat tgacaacaat accacgatta acatctagac ctgattcttt
    16741 ggctacttct gtgtatggtc gtatacctac tgccataaca actaagtctg ccggaatctc
    16801 gcgtccatca gccaatttaa caccctcaac atcatcttct cctaagattt cagttgtgtt
    16861 ggcttgcatt tcaaacttca taccttgctt ttctagatct gctttaagca tatttccagc
    16921 tttacggtct agctgcattt ccatcaacca ttcagctaaa tgtaacaccg ttacttccat
    16981 accttgatct aataaaccac gtgcacactc taaacctagt aagcctccac caattacaat
    17041 tgctttcttt ttagtcttcg caatgttcat catttgttca gtatcatcaa tcgttctcca
    17101 accaattaca ctcggtaatg tcgaacctgg aactggaatg acgaatgctt tagaacctgt
    17161 cgcaaaaatc aatttatcgt atgatacttc aataccattt gcagtagtaa ctgattgatt
    17221 tgctctatct acttcaatta caggatcatt tgtaattaac tcgataccat gttcctcata
    17281 ccactcatat ggattcataa ttgtttcttc aactgtcatt ttattttgta aaatatttga
    17341 aagcatgatg cggttatagt ttggataagg ttctttacct attaccgtaa tatcatataa
    17401 atcgttggcg cgctctaata tttcttcgat tgttcgaatg cccgccatac cgttaccaat
    17461 cattactagt ttttgctttg ccataaaata tgccccttta ctccataata tttatttcaa
    17521 aaaaaggtat taatttttcg ttagtgcttt tatattttca tcggaatcat taagctttct
    17581 aatctatcgt taatgatttg ctttaaaatt gggtcgaagt taattgaagg tgtgatgtgt
    17641 atatctgtat taataaccat gtcattcatt tgctgcttca ctttgttaac aagccttccg
    17701 tcgtataaaa ataatggtac gacaatcaat ttttgatacc gtttcgagat gctttctaaa
    17761 tcatgtgtaa aactaatctc tccatatagc gttctcgcat atgtcggctt gctaatttgc
    17821 aaattttgag tgcatatttg taactcttcg tgtgccttag taaactttcc attaatattg
    17881 ccgtgtgcaa caaccataac tccaacttgt tgttcttcac ctgctaatgc gtcacaaata
    17941 cgttgttcaa ttaatcgtct cattaaagga tgtgtgccaa gtggctcgct tacttctacc
    18001 tttatgtctg gataccgtcg tttcatttca tgaacgatat tcggtatatc cttgagataa
    18061 tgcattgcac taaagattag caatggtaca attttaaaat ggtcaacccc actttgaatc
    18121 aacgtcgtca ttaccgtctc taaatcctga tgctcacttt ctaaaaacgc aatatcatag
    18181 tgatgtatat catcttttac taattcagaa ataaatgctt ctaacgcttg attctgtcgt
    18241 ccgtgcctca tgccatgtgc aacaatgata ttcccattca catttaccaa ccctttcaca
    18301 cgtattgtat accaaatcat tttgtttttg tgaaaagaat cacattataa tgtaaaatca
    18361 gggaattccc tgatgcctgt agtcatgcat attccttata cattttccct ttttgttaaa
    18421 tcaaaaaagc gaccgatata tgaatcccta ctcaacattt atttgagcaa gcattaatat
    18481 atcggtcgct tgtagtgtat attattatct taaaatggtg gttggcctaa tattgtttcg
    18541 tcaaagcgct cgggtatcaa tactttgcgc atgatcacac ctaaatcgcc atcatcattt
    18601 tcatgttcgc tgtatatttc ataacctctt ttttcataaa ttttaagtaa ccacggatgc
    18661 aatcttgcag atgtacctaa agtaactgcc gctgacttta acgtatctcg caaaaatgct
    18721 tcttcaacat aagtaagtaa ttggctacca tagcctttcc cttcatactc aggatttgtc
    18781 gcaaaccacc agacaaaagg atagcccgaa atacttttca cacttcccca aggatatcta
    18841 accgtaatcg tagatataat ttcatcatca attgtcatga caaatgtagt atttttatct
    18901 atattttctt taacagcatc taaattagca ttaactgaag gccaatcaat acctagttct
    18961 cttagaggcg taaatgcttc atgcatgagt tgttgcaatt tttctgcatc ttgttcactt
    19021 gcgagtcgaa tcatcatttt ttgtcatatt aatccccact cttttttaaa tgatttaacc
    19081 atattttatt tttaaaataa atatccatca aagtgtatca ataaatttat cacatgttag
    19141 aaagtatgct tcatctgaat acaccaatac tctcataaaa cttattaaaa attactctct
    19201 caacgtaaaa aaaccattca aattcatgaa tggtttggaa gaatgattca ttgttacgct
    19261 atttaatcac tacatcttaa ttattgttgc tctaaacgat tacgcttacc atttaagaaa
    19321 gcataaacga gacctacaaa aataccgcca ccgacaaagt tacctaagaa agcaaaaacg
    19381 atatttttta aaacgtgtaa ccatgaaact gcatcaaggt taaagaatac catacctgca
    19441 tatagacctg cattgaacac aacgtgctca tatcccatgt atacaaagac cacgacacca
    19501 caagctataa agaatgcctt tgttaagccg cctttgaatt gcatagagat gaaaatacca
    19561 atattaataa agaagttaca gaaaatacct tttgtaaaaa tattcaacca tgttgaatca
    19621 acagtctttt tctgaactaa agctgttaaa gcttgtgtca tttcaggtgt catgacatgc
    19681 gcaaatttca ttaagaaaaa taacacaaac ccacctaaaa tatttcctaa gaaacaatat
    19741 aataaaatcc aagtcatctt cttaacagaa acgactttat aataccagcc tactgtaaag
    19801 tacatgaagt tacttgttaa taattcagag ttagttaata caactaaaat caaacctaaa
    19861 ctaaaagcaa tggctcccat taaattgata agtccatcta cgtgagtact cgcaaactgc
    19921 gtctttattc cgaacataaa tacagtaacg attgagagta aaaatccggc catcattgct
    19981 ttaagcatat atcggccagg tgtttgctgt accattactt gcttcatttg tactgaagat
    20041 gatattgaat caacaattgc gcctgttgaa tatgtatctt caactgtttt cttattttct
    20101 atcacagtac ttcccccatt tactttgatc aaataaaatt tactttctaa ttatattagt
    20161 aaatcaatgt aattgatagt ttaaatttca gaaaaatctt tttattctct caaaaatgta
    20221 gttttctttc ataaaaacag cgagataaat cgcgtgattg gctaatatct ttatgattta
    20281 agatattacg acgctcattt tatctcgctt tatgtaaagt attacttatt cttttttaaa
    20341 tttgaaaagt aatgtaaacc atatgcaaat acactgtaac caattgcagt acgtaaaata
    20401 aaccttataa ctttattagg tacacatttt gacgttactg aaatagaaga tagtagtgtt
    20461 gtgaaaatat ataatattaa aactttgata taacgttcta ccatcgaatc atccctcatt
    20521 tttataatat tacaagatta ttataaaccg aatgaaacaa aaaagttttt acatattttc
    20581 aacaacttta tttcaataat gcatcaagtt gatctaaagt ttgattcata ccttgttcaa
    20641 cacccatgtt tatgacttgt tgagcggctt cttttgttgg aaataccgat gttgatgtca
    20701 ctgtcgtttt cgttgtattg ctcttggaaa atgacaaagt aattttcatg cttggcattt
    20761 ttgtatcttt tgcaccttga ggtgtcgcaa atgagtcaat atattcaatt aaatatggac
    20821 gcttaaccgt tttatattct gctaatgtat agctggtcat tgtaggtgtt ttaatcgcat
    20881 aaaatgcatc accgcctgaa acagcattaa agcgaaacac tttggtacta gcgtcttttg
    20941 gatgaaacca tttttcaaat aatgcttttg tcgtataggc atcaaacacc ttttcaatcg
    21001 gtgcttctat cgttcttgaa aaaataattt tattatcttc aactttaatc gtcatcttcc
    21061 cactccctat cttttatatt tactcatctt aacatgcatt gagtaacata tgttaccaaa
    21121 tcatttgtta taaactatag taatattaat cattccctct gttgaaattg ataactacga
    21181 aataaaaaac acactctatt cagctattaa cagttgagta gagtgtgcaa acctgttgaa
    21241 ttaatgcgct aacatttctt ctttgatttg gtctttattt aattttgaag gataatatgt
    21301 tggccagtta tccatttctt ttaataacgc gtcattatca ttacccataa agatatggaa
    21361 gtgttctgcc tttttaggtg caatgttgtg atcgctaaat tggatgaatt tcggtaagtc
    21421 tttatcatta ccatcgacca atttaaacat aaatctcacg ccacggttac ctttaggata
    21481 tttcaatgtt ttcttaccat tgtattcata tttaccagtg tgtttcttac catctttagt
    21541 aaatgtaatt tcatttcctt ttatatcaat gttagtaata tcagttttat atcctttgtc
    21601 ataataagct tttaaatctt tagcagattt cttcggatca ttttcagctt tatgttccat
    21661 cacttcatca agcgtaccgt cttttaagta agggtaaaca gattgccatt caccagcata
    21721 atcgcttaat tcacggtctt taacttgctc atctttaaaa tacccatcag aaattgcttt
    21781 gtcatgttta ctttcagctt tgtcgtcttt cactttaaca ccactgtcta aagctttacc
    21841 gatattttca atattagatt tcattaatga ttgatacgta atattatctt ttttctgttg
    21901 ttctttattt aaagactcca tgttgtagaa ttttaaaggc ttcgcatctg tttctttacg
    21961 aattgtttca gtcactttat tcgcaacatt atcttcgtaa agaatatatt tagcattgct
    22021 atctctaatt tctttaacaa ttttagttaa ttctttttgt gatggatctt cagcattcat
    22081 gttttgaata cctttttgaa caaaaccata acgatcagct aagtatccaa ttgattcatg
    22141 tgaaatgaat actgcattac cttgcttatc ttttgtaact tgcttcatat cgttatcaat
    22201 tttcttaaga tcgtcgttta attttttgta gtttttctca tagtcatctt tatgttttgg
    22261 atcttttttc actaattcat ctttaatttc tttagcgaaa gtttgattaa ttttaggatc
    22321 taaccatacg tgtggatcat atccaccatg atgatgatgt tcttctttct catgatcatg
    22381 tccctcatgt tcatgctctt caccatgctc gtgttgatca gttaaaagct ttgctttatc
    22441 taatttatcc tctaaagaca gttttttatc tttatctttg atagtagatg caactttctt
    22501 agcaaccgga tctaaattat cccctgtata cataaacaag tctgacttgc ttgcacttaa
    22561 tatatctttt tgtgttggtt catagctatg taaatctgtc cctgctggat agattgatga
    22621 taccttcacg tgttttccac caatttgctc tgcaaatgat tgcaatggat aaactgtcgt
    22681 tttaattgtt accttgccat ctttatcttt tccatcatca ttcccacatg cggctaatga
    22741 taaagttacg gctggtacaa gaagtaacat acctaatttc tttttcatag tcaccctcct
    22801 aaatagtaat ctttacgttt taaaattata tactcatgct acttaaaata caagcactgt
    22861 ttttataaat aatataaagt cgaatcatac tttaggtatt taccactttt cgttttatct
    22921 aatacatgac tattgtacaa atatgataca gaaaacttta tagtatttac gaacgtcata
    22981 atataagaaa aaagacaatc tctatcatat tgctaataga aattgccttt tcatattgtc
    23041 ctaaaataac ttaccctatt tttatagtgt gacttttaga tttaataatg gtatttacat
    23101 gatgctattt ttatatgatt gtcatctact gtataaacca atctatgttc gtgattgata
    23161 cgtctactat aatacccagt cagattcgat tttaactttt caggcttacc tataccttcc
    23221 aatgcaccat ttctgtcaat acttttaagt aactcattaa ttttcttcac cattttttta
    23281 tcgttctgct gaaaatactt ataatcttca aaggcttgag gcgaaaacgt aatatttaac
    23341 ctagccatta tacatctata tctttcgtta tagttttccc acgttctaga tctacaattg
    23401 attgagctaa gtgttcagca ttatttgggt tctgttggag gtaaagtgtt tccatcatgg
    23461 agttataatc tgattctgac atgattacta cgtttttatc atcagtagat gttacagtta
    23521 ccatatcact atcatcattt acttttgtca taagtgcctt taaattctgt cgagcgtatg
    23581 aataattttt aataatcata tttatcgcct ccttttaaca cacctcaatt gtacaaatat
    23641 ctgtacaata aaacaagcga cacagaaaaa aactactaat caagatttcg cacggggaag
    23701 aatcaatacc tatcctgtac tgattcccta ctaaacaatg tttacatgat aatttctacc
    23761 tatttagact tcatacatta aaaagacaat ctctatcata tcgctaatag aaattgcctt
    23821 ttcatattgt cctaataaat tgatatcact tacttatctc aacaattaag actattttat
    23881 tttatctttt aataacttct cataactttc ataatcatat ggatcttcaa ctttctcgcc
    23941 attaataaat gcagttggtg tcgtttttat atgattatct ttcgctattt ttttatcttt
    24001 ctctgcagct ttccaagact tgctatcttt tgtcttgtaa tcttttataa ttttattttc
    24061 cgtctcttta tctaaatgca gttgtttaat atgtttatct aatagttctt ttgttaacca
    24121 ttctttattt tcatcttgct gggcagcaaa taattgcttt tgaaaatcta aaaatgattt
    24181 aggtgcatac atcaatactg catgactcgc acgcgaacca acaattgagt ctttacctaa
    24241 gaaagctaaa ttgacaaatt ggtattccac tttgtgatta tctatataat ttttacgcaa
    24301 ctttggcatg actttttcat ctaattcttt acaataagga catttatagt cgccatatat
    24361 gacaactaat ggtttgccgt ttttcgagga tgtcgttgct gattcttttt taccgcaagc
    24421 tgttaaaatt aacatgctca ctataaataa tgttagtaat tttttagtca taataactcc
    24481 tgttcatatc tagttcgtca tattttcttc atgataatcc gcatcatgtt gttgataata
    24541 tcgtctcgaa tcaaaatctt gcttatatga tacacgttta tcactaaaat catatgcata
    24601 ataatgcact tcatcatcat tactcaaagg tttataagct aaaatgataa ttttatcctt
    24661 atcatagaca taaatattag catctttgcg ttcaaaatga tcaacacgat caccgttgcc
    24721 ttttttggca agttgttctt gtttttgatt ttgaatttta actgcttcat ctatttcttt
    24781 cacatattta tcattaccac atcctgatat aaagactagt aataataata ccgtcgccac
    24841 aaatcgcttc aatgctccca ctcctactat caacttccaa ctaactttca atataattaa
    24901 tactatacac cgtcaaacat taatgcgcta caactactac caatgcattt aatataaata
    24961 tccaaagaaa taacagttat gtatagtcaa agaaaaatat atttcatttt ctatgtgtat
    25021 ttgtaatcag tttttgataa aataaaaaaa tagcattatt aataaattca aaaattacag
    25081 ttcaattcag agagggatgg gataaaatga actttgtaac gttgacttcg gatgagttca
    25141 atgcgtttac aacaaagcat ttttcacatt acacacaatc agctattcat tacaatcata
    25201 gagttgattt aaaaggcgat gtgcatcttg taggggttaa agatgacaat ggtcaagtga
    25261 ttgcaggatg cttattgaca gaagcacgca cacttaaatt tttcaaatat ttttatacac
    25321 atcgcgggcc agtgatggat tatacaaatc aatcattagt agcatttttc tttaaagcat
    25381 taacgtcata tttaaagaaa cacaattgtt tatatgtcct tgtagatcca tatttaattg
    25441 aaaatttacg caatgcagac ggtgaaattg ttaaatctta tgataaccga gcatttgtta
    25501 gaacaatgga taaattaggt tataaacacc aaggtttccc tgtaggttat gattcaatga
    25561 gccaaatccg ttggctgtca gtgttagatt taaaagataa gactgaagac caacttttaa
    25621 aagaaatgga ttatcaaacg agacgtaata ttaaaaaaac atatgatatt ggtgtcaaaa
    25681 ctaaaacgtt aacgattgat gaaacgcaaa cttttttcga cttattccat atggctgagg
    25741 aaaagcatgg tttcaaattc cgtgagttac catactttga agaaatgcaa aagttatacg
    25801 atgaccacgc catgttaaag ttggcgtata ttgatttaaa cgagtattta aaaacgttac
    25861 aattaaagca acaacaatta acagctgaac tctcaggtgt tgaagaagca ttggaagaaa
    25921 gtcctaattc aaagaaaaat aaaacgaaac gcacacagct agaacaacag ctaaatagca
    25981 ataagcgtaa aatcgacaat acaatagaac aaattgaaca agatggtgca gtcttaaact
    26041 tagcttctgc tttgtttatc tacaatgagc atgaagttta ttacttatct agtggttcaa
    26101 accctaaata taatgcttat atgggtgcct accatttgca gtgggaaatg attaagtttg
    26161 ctaaagcgca tcatattgat cgctataatt tttatggtat taccggtgac ttttcagagt
    26221 catcagaaga ttatggagtc caacaattca aaaaaggttt taacgcacat gtagaagagt
    26281 atatcggcga ctttattaag cctatcaaac cattactata taaggtacaa acatacttaa
    26341 atcataaacg tcgttaaaat atcgctccaa gattatgaca acataacaaa gaaccacgca
    26401 tcatgtatat ggcatcatca ccatactaaa acatgatacg tggttttatt tgattattct
    26461 tcatttataa catttaagaa acgtcttaat tcttctgttt tcggacgatt gaacatctct
    26521 tctggagcct cttgttctcc gatcatgcct tcatgaataa atacaatgtt attagatact
    26581 tctttagcaa aacgcatttc atgtgtcaca atgaccattg tcatgccttc attagccaaa
    26641 tctttaataa cctttaaaac atcattcaca agttcaggat caagtgcaga tgttggttca
    26701 tcaaacaaca tcactttagg gttcattgct agtgctcttg caatagcaac acgttgttgt
    26761 tgaccacctg ataatgcatg tggacgttga tctttgacat gtgttaaacc aactttctca
    26821 agtaactcaa gtgatttccc acgtgcctca tcctttttca actttttaac tgtgataaga
    26881 ccttccatta cattttctaa tgccgtctta tgcggaaaaa ggttataact ttgaaatacc
    26941 ataccagact gtttacgaac ttctatttgt gattttttat ctttagatgt atatgttttg
    27001 ccgttaacat aaactgtacc ttcagttgga atttctaatg cattaatcat acgtaacaat
    27061 gttgttttac ctgaaccaga tcgaccgatt aaggttacaa cctcaccttg ttcaacagat
    27121 aaatcaatac ctttgatgac ttcaacatca ttgaatgatt tatggatatt gttcaattga
    27181 atcatgagcg ataccctctt tcaatataag attcatagat accttggata attgaaataa
    27241 taaagcatac cacccagtac attagcgcaa ctaacacata aattgttaaa tattcatatg
    27301 ttgtagacgc aacttcttga gcttttctaa acatttcagc cactaaaata aatcctaata
    27361 atgatgtatc tttgattaaa cttaaaaatg tattacctag tgcaggaatc gacactcgaa
    27421 ttgcctgcgg taaaatgata cgttgtatcg tttgaccata cgtcataccg atggaatatg
    27481 cagcttctgt ttgtcctttc ggtatagaaa taataccgcc acgaataatt tccgaagcat
    27541 acgcacctac atttaatgat aaaccaataa tagcagccac tacaggtgcc aatgtccatt
    27601 ggttgtcagc gtcatttgtt aataatctac ctaattctgg aataccataa aatatgataa
    27661 atagttgtac tatcataggt gttcctcgaa taatagatac atagacacgt gaaatacttc
    27721 ttaaaatttt actcgttgaa attcgcatta atgctgtaaa taatgcaata atcaatccta
    27781 aaacaaatgt aactaatgta ataggaattg aatatttgac caaaccttct agcataggtc
    27841 cgaaagcttg ttttgcagca tctagcgcat gttgttgttc gctatttaga tttagaaaca
    27901 tcttgaccaa accatttctt acctatttta gctaattcac cgttttcctc gatttttttc
    27961 aagccatcat tgaatttttg aactgtttca tcatctgctt ttttagaaaa tgcaaatgca
    28021 gatctacttt gttcagcatt acctttgatt gctttgatct tagcattagg tttttgtttt
    28081 ttataatcca agtatgacag actatcatta aatgtaccat caacacgctt agacaataat
    28141 aaatccattg attggttaaa gccatcaact tttgtaatat cagcaccttt atccttagct
    28201 aatttaccat aattagatgt gaatgtttgt gctaacttct tacctttaac atcatcaaaa
    28261 tctttaatat ctttttcatt ttcacgaata actaaaacag cacttgagaa tgtgtaaggc
    28321 ttagaaaatt tatatttctt ttctctatca ggattaatac ctacttggtt cgcgattaca
    28381 tcaaaacgcc ctgcgtctaa acctgcaaac atagaatccc aagaagtttc attaaattta
    28441 agttttaaac cttcttcttt agccactgct ttaataacat caatatcgta accagttaat
    28501 ttaccatctt tatcgtggaa actaaatggt gcataagtcc cctcagtccc aacattaatt
    28561 gtcttgctat ctttactagc ttccttgtct ttcgacgaat tgtttccgca tgcagccaat
    28621 atgaaaacga aagctatcat cacaaataaa agtcttttca tagaatatat ctccttattc
    28681 ttattattct aatcggaata aaaatatttt aatctgaaat gccggataag tcaatatgtt
    28741 gaacttttgc taaaaagctt tatatagcta gcaacttata tttattgacg agaatcaact
    28801 tcttttttct tcccttgtaa cataaatgaa caaaagaaac taataactgt aatcaataat
    28861 gcaaataaaa atccagcgtg ataaccatgc aacatcgtat caactttaat ttgattcatc
    28921 atatcaattt tgttcatgcc attatagtct gacatagttg gagaaaagtt tttgcttgca
    28981 tgagataaaa tagtaattaa tgcagctgtt ccgatagaac cagcaatttg ttgtactgtg
    29041 tttgtcatag atgaaccatg tgcattcatt tctggcttta attgattcat tgtatgggtc
    29101 attactggaa ttaatcctaa cgcgatacca accattcgaa tcgcataaac tgttgccaac
    29161 atgattgttg atgtttgttc atccattaca acaaaataag ccgtagtaat aacaacaatc
    29221 gccataccga ctaaagccaa ttttctagga ccaaactttt catataaagc acccgaagtc
    29281 atcgacataa atgccataac aattgctcct ggtaataaag ttaacccaga atctaaagct
    29341 gaacgatgca ataaattttg cacaaagata ggtaaaatcg tttccgaacc aatcatcgat
    29401 accattgtga cacccataat aatgatacca actgtaaatt gtttattttt aaatacagca
    29461 aaatttaata atggtgttga tagccgatat tgacgtaata caaataatgc gactaaaaca
    29521 acgcctccaa tcatagataa taatacgata ggattatcaa aacctttttc tgaaattgaa
    29581 ctcgttccat acaataaccc gccgaaacct agtacagaaa aaatgacaga aataaaatca
    29641 ataggtactt taattgtagt tccaacattt tttatcgttt taaatccaat taaaaatgtc
    29701 acagctgcaa ttggagcgac aacatggaaa agcgatctcc agttaaaata ttcaacaagg
    29761 taacctgaaa gagtaggtcc aatagctggt gctaaaccaa ttaccaaacc aaatatcccc
    29821 atatatttac cgcgttcatg cggttggaaa acatccaaaa tcgttgtcat catcaatggc
    29881 atcataatac ctgcgcctaa cgcctgaata attctagcaa ctaataatgt tgcaaagtgc
    29941 ggactcaagc cgccgagtaa tgtacctaag aaaaagatag aaatacccac taaaaacact
    30001 tgtcttgttg tataacgttg tataacaagc gcggacaaag gaataacaat cccattagtc
    30061 aataaaaaag ctgttgtcag ccattgaact tgtgtatatg aaatttcaaa atctttcata
    30121 atacttggta aagccgtcac taatagtgtt tcatttaata atccaaaaaa tccacccatc
    30181 agcatagtaa taatcatcgt taatttttgt ttcttcgaca tttaaagctt ccccctcacc
    30241 atctaaaaaa cgacacacat ttttagatat ccggtaatta tacccatttt ccaaaaaatt
    30301 agcacgactt tattacaatt tctttaaaac gtaatcatta ctatttacat atgagtagtt
    30361 atcggtcata atatatttac aaggagggat agagatgaaa ttagatttac agactgctcg
    30421 tcgaaattta aatagcccta acattaaaac aagaaagcgt gctttaaaga ttattaagca
    30481 acacaaaaga gcgaaataat tgttttaatt tgactatatt gtatgtctaa ctatattttc
    30541 gagtatccct agctcgttaa tataaactct atacttaact atttccttac attacttcac
    30601 atatttaaga gctgcatata cactcactcc ttgtgtacat gcagctcttt ttttataagt
    30661 agtatttatc tataacttct aaatcatccg ttaattcata aacaagcggt gcacctgttt
    30721 taatttcata attaatgata tcttcatctg acacatcttc aagatattta attaatgcgc
    30781 gaattgaatt tccgtgtgca gaaactaata ccgtttgacc atctagcaaa tattgtgaaa
    30841 tatgatctgt ccaaaatggt atcactcgaa ctaaagtatc tttcagactt tcagaataag
    30901 gcatcatacg tttatctaaa tgattatatc gacgatcagc taagtaagct tcacgttgtt
    30961 cttcggtttc agcaggtggt ttcacatcat aagaacgacg ccaaatatgt acttgttctt
    31021 ctccaaattc ttttctagca tcatctttat ttaagccttg caatccacca tagtggcgtt
    31081 catttaaacg ccagctttta tatacaggaa tccattgttg tttagattca gttaaaatat
    31141 aatgcgttgt atctaaagca cgtgttaata acgatgtaaa agctacatcg atggcaatgt
    31201 tattttctct tactttttca cctgctctag tcgcttcatt aataccttgt tcagataaat
    31261 taacatcttc ccatccagta aataagtttt tagcattcca ctcgctttgt ccatgacgac
    31321 ataaaattaa ttttggcata acttataacc tctttccttt aatctttagg acgttatttc
    31381 agggatattt taagatgatt taatcttagt aattttctt
//
LOCUS       DBNENW010000013        29036 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNENW010000013 DBNENW010000000
VERSION     DBNENW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.013"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            562..1899
                     /locus_tag="AC1LW8_000484"
     CDS             562..1899
                     /locus_tag="AC1LW8_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIP4772349.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQVLAVLPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1901..2518
                     /gene="tmk"
                     /locus_tag="AC1LW8_000485"
     CDS             1901..2518
                     /gene="tmk"
                     /locus_tag="AC1LW8_000485"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIP4772350.1"
                     /translation="MSAFITFEGPEGSGKTTVINKVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2546..2875
                     /locus_tag="AC1LW8_000486"
     CDS             2546..2875
                     /locus_tag="AC1LW8_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIP4772351.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            3089..4015
                     /locus_tag="AC1LW8_000487"
     CDS             3089..4015
                     /locus_tag="AC1LW8_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HIP4772352.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            4016..4819
                     /locus_tag="AC1LW8_000488"
     CDS             4016..4819
                     /locus_tag="AC1LW8_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIP4772353.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRVADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4836..5183
                     /gene="yabA"
                     /locus_tag="AC1LW8_000489"
     CDS             4836..5183
                     /gene="yabA"
                     /locus_tag="AC1LW8_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HIP4772354.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5457..6182
                     /locus_tag="AC1LW8_000490"
     CDS             5457..6182
                     /locus_tag="AC1LW8_000490"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIP4772355.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYITMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6175..6423
                     /locus_tag="AC1LW8_000491"
     CDS             6175..6423
                     /locus_tag="AC1LW8_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIP4772356.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6425..7264
                     /gene="rsmI"
                     /locus_tag="AC1LW8_000492"
     CDS             6425..7264
                     /gene="rsmI"
                     /locus_tag="AC1LW8_000492"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIP4772357.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7549..9522
                     /gene="metG"
                     /locus_tag="AC1LW8_000493"
     CDS             7549..9522
                     /gene="metG"
                     /locus_tag="AC1LW8_000493"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIP4772358.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIINAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9553..10326
                     /locus_tag="AC1LW8_000494"
     CDS             9553..10326
                     /locus_tag="AC1LW8_000494"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIP4772359.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10493..11029
                     /gene="rnmV"
                     /locus_tag="AC1LW8_000495"
     CDS             10493..11029
                     /gene="rnmV"
                     /locus_tag="AC1LW8_000495"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIP4772360.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            11040..11933
                     /gene="rsmA"
                     /locus_tag="AC1LW8_000496"
     CDS             11040..11933
                     /gene="rsmA"
                     /locus_tag="AC1LW8_000496"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIP4772361.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            12033..12296
                     /gene="veg"
                     /locus_tag="AC1LW8_000497"
     CDS             12033..12296
                     /gene="veg"
                     /locus_tag="AC1LW8_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation stimulator Veg"
                     /protein_id="HIP4772362.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12606..13454
                     /gene="ispE"
                     /locus_tag="AC1LW8_000498"
     CDS             12606..13454
                     /gene="ispE"
                     /locus_tag="AC1LW8_000498"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIP4772363.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13468..14292
                     /gene="purR"
                     /locus_tag="AC1LW8_000499"
     CDS             13468..14292
                     /gene="purR"
                     /locus_tag="AC1LW8_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HIP4772364.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14309..14689
                     /locus_tag="AC1LW8_000500"
     CDS             14309..14689
                     /locus_tag="AC1LW8_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIP4772365.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGEIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14761..15063
                     /gene="spoVG"
                     /locus_tag="AC1LW8_000501"
     CDS             14761..15063
                     /gene="spoVG"
                     /locus_tag="AC1LW8_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIP4772366.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15253..15384
                     /locus_tag="AC1LW8_000502"
     CDS             15253..15384
                     /locus_tag="AC1LW8_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772367.1"
                     /translation="MCLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15407..16759
                     /gene="glmU"
                     /locus_tag="AC1LW8_000503"
     CDS             15407..16759
                     /gene="glmU"
                     /locus_tag="AC1LW8_000503"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIP4772368.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16906..17871
                     /locus_tag="AC1LW8_000504"
     CDS             16906..17871
                     /locus_tag="AC1LW8_000504"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIP4772369.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            18021..18674
                     /locus_tag="AC1LW8_000505"
     CDS             18021..18674
                     /locus_tag="AC1LW8_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIP4772370.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18985..19557
                     /gene="pth"
                     /locus_tag="AC1LW8_000506"
     CDS             18985..19557
                     /gene="pth"
                     /locus_tag="AC1LW8_000506"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIP4772371.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19557..23063
                     /gene="mfd"
                     /locus_tag="AC1LW8_000507"
     CDS             19557..23063
                     /gene="mfd"
                     /locus_tag="AC1LW8_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIP4772372.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTKIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLISRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            23053..24579
                     /locus_tag="AC1LW8_000508"
     CDS             23053..24579
                     /locus_tag="AC1LW8_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIP4772373.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAINMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24579..25772
                     /locus_tag="AC1LW8_000509"
     CDS             24579..25772
                     /locus_tag="AC1LW8_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIP4772374.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            25769..26032
                     /locus_tag="AC1LW8_000510"
     CDS             25769..26032
                     /locus_tag="AC1LW8_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIP4772375.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            26050..26442
                     /gene="divIC"
                     /locus_tag="AC1LW8_000511"
     CDS             26050..26442
                     /gene="divIC"
                     /locus_tag="AC1LW8_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIC"
                     /protein_id="HIP4772376.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26547..26948
                     /locus_tag="AC1LW8_000512"
     CDS             26547..26948
                     /locus_tag="AC1LW8_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HIP4772377.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            27128..28423
                     /gene="tilS"
                     /locus_tag="AC1LW8_000513"
     CDS             27128..28423
                     /gene="tilS"
                     /locus_tag="AC1LW8_000513"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIP4772378.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEVYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKASHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            28428..28967
                     /gene="hpt"
                     /locus_tag="AC1LW8_000514"
     CDS             28428..28967
                     /gene="hpt"
                     /locus_tag="AC1LW8_000514"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIP4772379.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT        10520 a         3895 c         5802 g         8819 t
ORIGIN      
        1 aaataaaaag caaacacaaa gaaaaatggc ttggcgaagt gaaaacgttt gaatctgacg
       61 aaacgagaaa agagcgcaac gagtttagta gagctaaatg agtaagtgag agccgaaaaa
      121 gaggaaagaa gcaagcaatt gtcacaagtc aagaaaggtt cttagcgacg atggtagcca
      181 acttacgttc cgctagagta gaacgctgct aggcaagaaa tggatgcgat gagccgcatt
      241 gagttttgga aatgatattt taataatgtg cacttttgat tgtttaaatc tgtgactaga
      301 agtataagtg aagacattca gaagtattat aaaaagtgaa cagcagtaag atagttttta
      361 atcataaatc atcttactgc tgtttttagg ttttatgtct aatatttttt aaatcgaaat
      421 acaaaaagga aattaattat tatacaatag acaagctatt gcataagtaa cactaacttt
      481 tgtcaaagaa gtgttactat ataattaata cttttgaaag taactaattc caaaacagtg
      541 taaataaagg aagcgtatat catgaagcaa cctattttaa ataaattaga aagtttaaat
      601 caagaagaag cgatttcttt gcatgttccg ggtcataaaa atatgactat cggtcattta
      661 tctcaattat caatgacaat ggataaaact gaaatacctg gattagatga tttacatcat
      721 cctgaagaag tcattttgga aagtatgaag caggtggaga aacattcaga ttatgatgct
      781 tatttcttag tgaatggcac cacttcagga atattatctg tcatccagtc tttttcacag
      841 aaaaaaggcg atatcttaat ggcaagaaat gtacataaat ctgtattaca tgcgctcgat
      901 attagccaac aagaagggca ttttattgaa acgcatcaaa gtccgttaac gaatcattat
      961 aataaagtta atttaagccg tttgaataat gacggtcaca aacttgctgt gttgacttat
     1021 cctaactatt acggtgaaac atttaatgta gaagaggtta tcaaatcttt gcaccaatta
     1081 aatattcctg tactcattga cgaagcacac ggcgcgcact ttggattgca aggatttcca
     1141 gattctacat taaattatca agctgactat gttgttcaat cttttcataa aacgttacca
     1201 gctttaacga tgggctcggt actttatatt cataaaaatg caccttatag agaaactatt
     1261 atagaatatc taagctactt ccaaacatct agtccttcgt atttgattat ggctagttta
     1321 gagtcagctg ccgagttcta taaaacatat gatagtaccg tgttttttga taagagagcg
     1381 caattaatcg aatgtttgga gaagaagggt tttgaaatgc ttcaagttga tgatccgttg
     1441 aagttgctga taaaatatga aggttttaca ggtcatgata ttcaaaattg gtttatgaat
     1501 gcacatatct atttagaatt agcggacgac tatcaagtat tagcggtatt gccgttatgg
     1561 catcatgatg atacgtattt atttgattcg cttttacgta aaattgaaga tatgatttta
     1621 ccgaaaaaat cagtttctaa agttaaacaa acacaacttt taacaactga aggtaactat
     1681 aaaccaaaac gctttgaata tgttacttgg tgtgatttga aaaaggcaaa aggtaaagtt
     1741 ctggcgcgac atattgtccc gtatccgcca gggattccta ttattttcaa aggagaaaca
     1801 ataactgaaa atatgataga attagtaaat gaatatctgg aaactggaat gatagttgaa
     1861 ggaattaaaa ataataaaat tttagttgag gatgaataaa atgtcagctt ttataacttt
     1921 tgagggccca gaaggctctg gaaaaacaac tgtaattaat aaagtttacc atagattagt
     1981 aaaagattat gatgtcatta tgacaagaga accgggcggc gttcctactg gtgaagaaat
     2041 acgtaaaatt gtattagaag gcaatgatat ggacattaga actgaagcaa tgttatttgc
     2101 tgcatctaga agagaacatc ttgtattaaa ggtcatacca gctttaaaag aaggtaaggt
     2161 tgtgttgtgt gatcgctata tcgatagttc attagcttat caaggttatg ctagagggat
     2221 tggcgttgaa gaagtaagag cattaaacga atttgcaata aatggattat atccagactt
     2281 gacgatttat ttgaatgtta gtgctgaagt aggccgcgaa cgtattatta aaaattcaag
     2341 agatcaaaat agattagatc aagaagattt aaagtttcac gaaaaagtaa ttgaaggtta
     2401 ccaagaaatc attcataatg aatcacaacg gttcaaaagc gttaatgcag atcaacctct
     2461 tgaaaatgtt gttgaagaca cgtatcaaac tatcatcaaa tatttagaaa agatatgata
     2521 taattgttag aagaggtgtt ataaaatgaa aatgattata gcgatcgtac aagatcaaga
     2581 tagtcaggaa cttgcagatc aacttgttaa aaataacttt agagcaacaa aattggcaac
     2641 aacaggtggg tttttaagag caggtaatac aacattctta tgtggtgtca atgatgaccg
     2701 tgtagatgaa atattgtctg tgattaatca aacgtgtggt aatagagaac agttggtttc
     2761 acctattaca cctatgggag gcagtgcgga ttcgtacatt ccatatccag ttgaagttga
     2821 agttggcggt gctactgtat ttgttatgcc agttgatgca ttccatcaat tttaattcta
     2881 taatacaatc atcaattaga gatacttaaa atagtgtatt aataagttat taacaatttt
     2941 gggttgcttg actgcgacta gttcagatgc caatagattt gatttttgtg gttctaaaaa
     3001 taatcacaaa tcatggtcgc tattgttgca gtaattagtt gctctttggc aaccttttta
     3061 tataaaagca aaagggagtt tgtaatgaat ggatgaacag caacaattga cgaatgcata
     3121 tcattcaaat aaattatcgc atgcctattt atttgaaggt gatgatgcac aaacgatgaa
     3181 acaagttgcg attaattttg caaagcttat tttatgtcaa acagatagtc aatgtgaaac
     3241 aaaggttagt acatataatc atccagactt tatgtatata tcaacaactg agaatgcaat
     3301 taagaaagaa caagttgaac aacttgtgcg tcatatgaat caacttccta tagaaagcac
     3361 aaataaagtg tacatcattg aagactttga aaagttaact gttcaagggg aaaacagtat
     3421 cttgaaattt cttgaagaac caccggacaa tacgattgct attttattgt ctacaaaacc
     3481 tgagcaaatt ttagacacaa tccattcaag gtgtcagcat gtatatttca agcctattga
     3541 taaagaaaag tttataaata gattagttga acaagacatg tctaagccag tagctgaaat
     3601 gattagtact tatactacgc aaatagataa tgcaatggct ttaaatgaag aatttgattt
     3661 attagcatta aggaaatcag ttatacgttg gtgtgaattg ttgcttacta ataagccaat
     3721 ggcacttata ggtattattg atttattgaa acaggctaaa aataaaaaac tgcaatcttt
     3781 aactattgca gctgtgaatg gtttcttcga agatatcata catacaaagg taaatgtaga
     3841 ggataaacaa atatatagtg atttaaaaaa tgatattgat caatatgcgc aaaagttgtc
     3901 gtttaatcaa ttaattttga tgtttgatca actgacggaa gcacataaga aattgaatca
     3961 aaatgtaaat ccaacgcttg tatttgaaca aatcgtaatt aagggtgtga gttagatgcc
     4021 aaatgtaata ggtgttcagt ttcaaaaagc gggaaaatta gaatattata cacctaatga
     4081 tatacaagta gatatagatg actgggtagt tgtcgaatct aaaagaggca tagagatagg
     4141 tattgttaaa aatccattaa tggatattgc tgaagaggat gttgtgttac ctcttaaaaa
     4201 tattattcgc gttgctgatg acaaagatat tgataaattt aattgtaatg aacgagatgc
     4261 tgaaaatgca ttaatactat gtaaagacat tgtaagagaa caaggtttgg acatgcgttt
     4321 agtcaattgc gaatatacat tagataaatc gaaagttatt tttaatttta cggcggatga
     4381 tcgtattgat tttagaaaat tagtaaaaat attagcgcaa catttaaaaa cacgtatcga
     4441 gttgagacaa attggtgtaa gggatgaagc caaattgctt ggcggtatcg gaccttgtgg
     4501 taggtcgtta tgttgttcta catttttagg agattttgaa ccagtatcga ttaagatggc
     4561 taaggatcaa aatttatcat taaatccaac taaaatttct ggtgcatgtg gtcgtttgat
     4621 gtgttgttta aaatatgaaa atgactatta tgaggaagta cgtgcacaat tacctgatat
     4681 tggtgaagca attgaaacgc ctgatggtaa cgggaaagta gttgctttaa atatattaga
     4741 catttctatg caggtgaagc ttgagggaca tgaacagcca cttgaatata aattagaaga
     4801 aatagaaact atgcattaag gaggcattat tacatttgga tcgcaatgaa atatttgaaa
     4861 aaataatgcg tttagaaatg aatgtcaatc aactttcaaa ggaaacatca gaattaaagg
     4921 cacttgcagt tgaattagta gaagaaaatg tagcgcttca acttgaaaat gataatttga
     4981 aaaaggtgtt gggcaatgat gaaccaacta ctattgatac tgcgaattca aaaccagcaa
     5041 aagctgtgaa aaagccatta ccaagtaaag ataatttggc tatattgtat ggagaaggat
     5101 ttcatatttg taaaggcgaa ttatttggaa aacatcgaca tggtgaagat tgtctgttct
     5161 gtttagaagt tttaagtgat taatcaagca cactcaaata gtgttataat tataaatgaa
     5221 tatggtttgg ataagtctga gacaatgcat gtttcaggct ttaattgtgt ataaagtttt
     5281 ggtgattgca taagagatgg cggtactaaa tgttattatt aagtgtgcac gcagtatcat
     5341 tagttataaa atgtagctgt taaaagtcaa aaatacatcg aatgtagtta gtcatataat
     5401 ataaaaagag atttcaatta ctcaatagaa aaaggttgtc ttcataggag ttaaaaatgt
     5461 taaaagagaa tgaacgattt gatcaactaa tcaaagaaga ttttagtatt attcaaaatg
     5521 atgatgtttt ttcgttttca acggatgctt tgttgttagg gcattttaca aaacctagaa
     5581 caaaagatat tgtgttggac ttatgttcag gcaatggggt gataccgttg ttattgtttg
     5641 cgaaacatcc acgacatata gaaggtgttg agattcaaaa aacacttgtc gatatggcgc
     5701 gacgcacatt tcaattcaat gatgttgatg aatatataac aatgcatcac atggatttga
     5761 aaaacgttac taaagtattt aaaccttcac aatatacttt agtaacgtgt aatccacctt
     5821 attttaaaga gaatcagcaa caccaacatc aaaaagaagc acataagata gcgagacatg
     5881 agattatgtg tacacttgaa gattgcatga ttgcagcccg tcatttatta aaagaaggtg
     5941 gcaggctaaa catggtacat cgtgcagaga gactaatgga tgtcttgttt gaaatgagaa
     6001 aagtgaatat tgaacctaag aaagtcgttt ttatatatag taaagtaggg aaatcagcac
     6061 aaacgatagt agtagaaggt cgaaaaggtg gaaatcaagg tttagaaatc atgcccccat
     6121 tttatattta taatgaagat ggtaattata gcgaagaaat gaaggaagta tattatggat
     6181 agtcattttg tatatattgt aaaatgtagt gatggaagtt tatatacagg atacgctaaa
     6241 gacgttaatg cacgtgttga aaaacataac cgaggtcaag gagccaaata tacgaaagta
     6301 agacgtccgg tgcatttagt ttatcaagaa atgtatgaga caaagtctga agcattgaag
     6361 cgtgaatatg aaattaaaac ttataccaga caaaagaaat tgcgattaat taaggagcga
     6421 tagtatggct gtattatatt tagtgggcac accaattggt aatttagcag atattactta
     6481 tagagcagtt gatgtattga aacgtgttga tatgattgct tgtgaagaca ctagagtaac
     6541 tagtaagctg tgtaatcatt atgatattcc aactccatta aagtcatatc acgaacataa
     6601 caaggataag cagactgctt ttatcattga acagttagaa ttaggtcttg acgttgcgct
     6661 cgtatctgat gctggattgc ccttaattag tgatcctgga tacgaattag tagtggcagc
     6721 cagagaagct aatattaaag tagagactgt gcctggacct aatgctgggc tgacggcttt
     6781 gatggctagt ggattacctt catatgtata tacattttta ggatttttgc cacgaaaaga
     6841 gaaagaaaaa agtgctgtat tagagcaacg tatgcatgaa aatagcacat taattatata
     6901 cgaatcaccg catcgtgtga cagatacatt aaaaacaatt gcaaagatag atgcaacacg
     6961 acaagtatca ctagggcgtg aattgactaa gaagttcgaa caaattgtaa ctgatgatgt
     7021 aacacaatta caagcattga ttcagcaagg cgatgtacca ttgaaaggcg aattcgttat
     7081 cttgattgag ggtgctaaag cgaacaatga gatatcgtgg tttgatgatt tatctatcaa
     7141 tgagcatgtt gatcattata ttcaaacttc acagatgaaa ccaaaacaag ctattaaaaa
     7201 agttgctgaa gaacgacaac ttaaaacgaa tgaagtatat aatatttatc atcaaataag
     7261 ttaatcactt tatcgattat atgaaatttt aaacgatttt ataaacgcaa gctgtaattt
     7321 taaatggtaa gttatcattt tgtattgata ctgataaaat gatgttgact atgataaaaa
     7381 aatgatgaca tcgacgtttt ttaatgtaaa ataaatacat tgaaagtaat aaatacctta
     7441 acattgaata agatgaaaat gagatgacga gataaatgtt cgcgtccgtt gaaatgcata
     7501 gaaatcttag atattatttg aagtgagaca ttacgaggag gaacagttat ggctaaagaa
     7561 acattttata taacaacccc aatatactat cctagtggga atttacatat aggacatgca
     7621 tattctacag tggctggaga tgttattgca agatataaga gaatgcaagg atatgatgtt
     7681 cgttatttga ctggaacgga tgaacacggt caaaaaattc aagaaaaagc tcaaaaagct
     7741 ggtaagacag aaattgaata tttggatgag atgattgctg gaattaaaca attgtgggct
     7801 aagcttgaaa tttcaaatga tgattttatc agaacaactg aagaacgtca taaacatgtc
     7861 gttgagcaag tgtttgaacg tttattaaag caaggtgata tctatttagg tgaatatgaa
     7921 ggttggtatt ctgttccgga tgaaacatac tatacagagt cacaattagt agacccacaa
     7981 tacgaaaacg gtaaaattat tggtggcaaa agtccagatt ctggacacga agttgaacta
     8041 gttaaagaag aaagttattt ctttaatatt agtaaatata cagaccgtct attagaattc
     8101 tatgaccaaa acccagattt tatacaacca ccatcaagaa aaaatgaaat gattaacaac
     8161 ttcattaaac caggacttgc tgatttggct gtttctcgta catcatttaa ttggggtgtc
     8221 catgttccgt ctaatccaaa acatgttgtt tatgtttgga ttgatgcgtt agttaactat
     8281 atttcagcat taggctattt atcagatgat gagtcactat ttaacaaata ctggccagca
     8341 gatattcatt taatggctaa ggaaattgtg cgattccact caattatttg gcctatttta
     8401 ttgatggcat tagacttacc gttacctaaa aaagtctttg cacatggttg gattttgatg
     8461 aaagatggaa aaatgagtaa atctaaaggt aatgtcgtag accctaatat tttaattgat
     8521 cgctatggtt tagatgctac acgttattat ctaatgcgtg aattaccatt tggttcagat
     8581 ggcgtattta cacctgaagc atttgttgag cgtacaaatt tcgatctagc aaatgactta
     8641 ggtaacttag taaaccgtac gatttctatg gttaataagt actttgatgg cgaattacca
     8701 gcgtatcaag gtccacttca tgaattagat gaagaaatgg aagctatggc tttagaaaca
     8761 gtgaaaagct acactgaaag catggaaagt ttgcaatttt ctgtggcatt atctacggta
     8821 tggaagttta taagtagaac gaataagtat attgacgaaa caacgccttg ggtattagct
     8881 aaggacgata gccaaaaaga tatgttaggc aatgtaatgg ctcacttagt tgaaaatatt
     8941 cgttatgcag ctgtattatt acgtccattc ttaacacatg cgccgaaaga gatttttgaa
     9001 caattgaaca ttaacaatcc tcaatttatg gaatttagta gtttagagca atatggtgtg
     9061 cttactgagt caattatggt tactgggcaa cctaaaccta ttttcccaag attggatagc
     9121 gaagcggaaa ttgcatatat caaagaatca atgcaaccgc ctgctactga agaggaaaaa
     9181 gaagagattc ctagcaaacc tcaaattgat attaaagact ttgataaagt tgaaattaag
     9241 gcagcaacga ttattaatgc tgaacatgtt aagaagtcag ataagctttt aaaaattcaa
     9301 gtagacttag attctgaaca aagacaaatt gtatcaggaa ttgccaaatt ctatacacca
     9361 gatgatatta ttggtaaaaa agtagcagtt gttactaacc tgaaaccagc taaattaatg
     9421 ggacaaaaat ctgaaggtat gatattatct gctgaaaaag atggtgtatt aaccttagta
     9481 agtttaccaa gtgcaattcc aaatggtgca gtgattaaat aactgtattt ttaaaaatta
     9541 ggagagataa ttatgttaat cgatacacat gtccatttaa atgatgagca atacgatgat
     9601 gatttgagtg aagtgattac acgtgctaga gaagcaggtg ttgatcgtat gtttgtagtt
     9661 ggttttaaca aatcgacaat tgaacgcgcg atgaaattaa tcgatgagta tgatttttta
     9721 tatggcatta tcggttggca tccagttgat gcaattgatt ttacagaaga acacctggaa
     9781 tggattgaat ctttggctca gcatccaaaa gtgattggta ttggtgaaat gggattagat
     9841 tatcactggg ataaatctcc tgcagatgtt caaaaggaag tttttagaaa gcaaattgct
     9901 ttagctaagc gtttgaagtt accaattatc attcataacc gtgaagcaac tcaagactgt
     9961 atcgatatct tattggagga gcatgctgaa gaggtaggcg ggattatgca tagctttagt
    10021 ggttctccag aaattgcaga tattgtaact aataagctga atttttatat ttcattagga
    10081 ggacctgtga catttaaaaa tgctaaacag cctaaagaag ttgctaagca tgtgtcaatg
    10141 gagcgtttgc tagttgaaac cgatgcaccg tatctttcgc cacatccgta tagagggaag
    10201 cgaaatgaac cggcgagagt aactttagta gctgaacaaa ttgctgaatt aaaaggctta
    10261 tcttatgaag aagtgtgcga acaaacaact aaaaatgcag agaaattgtt taatttaaat
    10321 tcataaagtt aaaagtgaga aagatcaccg ccataaatgt aaacgatgct atattcgttt
    10381 aatatcctat ggttctttct cactttttta aattaaaata tcgtgcatgt ggaatacgtg
    10441 cgatagagat ggttagagct ttgaaattaa gaattgtagg aaggcgtttt aaatgaaaat
    10501 caatgagttt atagttgtag aaggacgaga tgatactgag cgtgttaaac gagctgttga
    10561 atgtgatacg attgaaacga atggtagtgc catcaacgaa caaactttag aagtaattag
    10621 aaatgctcaa caaagtcgag gcgttattgt attaacagat ccagatttcc caggagataa
    10681 aattagaagt acaattactg aacatgtcaa aggtgttaaa catgcgtata ttgatagaga
    10741 aaaagctaaa aataaaaaag ggaaaattgg tgttgaacat gccgacttaa ttgatattaa
    10801 agaagcgtta atgcatgtta gttcaccctt tgatgaagct tatgaatcaa ttgataaatc
    10861 tgtgctaata gagttggggt taatcgttgg gaaagatgca aggcgccgta gagaaatttt
    10921 aagtagaaaa ttgcgaatcg gccattccaa tggtaagcag ttattgaaaa agttaaatgc
    10981 atttggttat accgaagcgg atgtaaggca agctttagaa gatgaatgag gaagtgaaaa
    11041 tgttggataa taaagatatt gcaacaccat caagaacgcg agcgttgtta gataaatatg
    11101 gctttaattt taaaaaaagt ttaggacaga actttttgat agatgtgaat atcattaata
    11161 atatcattga tgcaagtgat attgatgcac aaactggggt gattgaaatt ggtccaggca
    11221 tggggtcatt gacagaacaa ttggctagac atgctaaaag agtattggca tttgaaattg
    11281 atcaacgttt aatacctgta ttaaatgata cactatcacc ttatgataat gtgacggtga
    11341 ttaatgaaga tattttaaaa gcgaatatta aagaagctgt tgaaaatcat ttacaagatt
    11401 gcgaaaaaat aatggttgtt gcaaacctgc cgtactatat tacgacgcca attttattaa
    11461 atttaatgca acaagatata ccaattgatg gctacgtggt gatgatgcaa aaagaagtgg
    11521 gcgaacgctt aaatgctgaa gtaggttcaa aagcatatgg ttcgttatca attgtcgtac
    11581 aatactatac agagactagt aaagtattaa cggtacctaa atctgtattt atgccaccac
    11641 ctaatgttga ttcaatagtt gtaaaactga tgcagagaac tgaaccgtta gtaacagtag
    11701 ataacgagga agcattcttt aagttagcaa aagcagcatt tgcacaaaga agaaagacaa
    11761 ttaacaataa ctatcaaaat tattttaaag atggtaaaca acacaaagaa gtgattttac
    11821 aatggttgga acaagcaggt attgatccaa gacgtcgcgg tgaaacgcta tctattcaag
    11881 attttgctaa attgtatgaa gaaaagaaaa aattccctca attagaaaat taaatgattg
    11941 acaaagcaaa gcactattgt taaaatttaa attttgtttg acgaaaacgc tgcaaatatg
    12001 gtattatgta acttgtagcg aggtggagca atatgccaaa atcaattttg gacatcaaaa
    12061 attctattga ttgtcatgta ggaaatcgta ttgtactgaa agccaatgga ggccgtaaga
    12121 aaacaataaa acgttctgga attttaaaag aaacatatcc gtcagttttc attgttgagt
    12181 tagatcaaga caaacacaac tttgagagag tatcttatac atacactgat gtgttaactg
    12241 aaaatgttca agtttcattt gaagaggata atcatcacga atcaattgca cactaaataa
    12301 gacatataga gatgttagac gtttcttagt ataagaagta aatattatga taattatttg
    12361 agtgttgggc gttatgttca atactctttt tatttacaaa atgtttaaca ctgatgtttc
    12421 gcttatagat ttttcagtaa atggatactt gtatttataa acacaaatac aagtaaatac
    12481 taagtaatta gatggagaaa attacttttt tattaaaaaa acactaaaaa acaaattaaa
    12541 atgtcaaata ttaattctct ttatgttaaa atcatcatat taagataacg aaaagagggc
    12601 ggaaaatgat atatgaaacg gcaccagcca aaattaattt tacgctcgat acacttttta
    12661 aaagaaatga tggctatcat gagattgaaa tgataatgac aacagttgat ttaaatgatc
    12721 gtttaacttt tcataaaaga aaagatcgaa agatagttgt tgagattgaa cataattatg
    12781 tgccttctaa tcataaaaat ctcgcatatc gtgcagcgca actatttatt gagcaatatc
    12841 aactaaagca aggtgtaaca atttctatcg ataaagaaat acctgtttct gctggcttag
    12901 ctggaggttc ggctgatgca gcagcaacgt taagaggatt gaatcgactt tttgatatag
    12961 gggcgagttt ggaagaattg gctctactag gcagtaaaat cgggacagat attccgtttt
    13021 gtatttataa taaaactgca ctatgtactg gaagaggaga gaaaatcgag tttttaaata
    13081 aaccaccttc agcttgggtg attcttgcta aaccaaactt aggcatatca tcaccagata
    13141 tatttaagtt gatcaattta gataagcgtt acgacgtaca tacgaaaatg tgttatgagg
    13201 ccttagaaaa tcgagattat caacaattat gtcaaagttt gtctaatcga ttagagccaa
    13261 tttctgtttc aaaacaccca caaatcgata aattaaaaaa taatatgttg aaaagtggtg
    13321 cagatggtgc gttaatgagt ggaagcggac cgactgtgta tgggctagca cgaaaagaaa
    13381 gccaagcaaa aaatatttat aatgcagtta acggttgttg taatgaagtg tacttagtta
    13441 gactattagg atagaagggt tgaaaagatg agatataaac gaagcgagag aattgttttt
    13501 atgacgcaat atttgatgaa ccatccgaat aaattgattc cattaacttt ttttgtgaaa
    13561 aaatttaaac aggcgaagtc ttcaataagt gaagatgtcc aaattataaa aaatacattc
    13621 caaaaagaaa agttaggtac agtaattact actgctggcg caagtggtgg tgttacgtat
    13681 aaaccaatga tgagtaaaga agaggcgact gaagttgtta atgaggtcat tactctatta
    13741 gaagagaaag aacgtttgtt acctggcggg tatttatttt tatcagattt ggtaggtaat
    13801 ccatcgctac taaacaaagt tggtaagtta attgccagta tttacatgga agaaaaatta
    13861 gatgctgttg ttaccattgc gacaaaaggt atttcattgg caaatgcggt tgctaatatt
    13921 ttaaatttac cagtagtagt gattagaaaa gacaacaagg tgactgaagg ttctacagtt
    13981 tcaattaatt acgtttcagg atcttcaaga aaaatagaga caatggtact ttcgaagaga
    14041 acattagcag aaaattcaaa tgttttagtt gtcgatgatt ttatgagggc tggtggctct
    14101 attaatggtg ttatgaattt aatgaatgag tttaaagccc atgtaaaagg ggtatcagta
    14161 cttgtagaat caaaagaagt taaacaaaga ttgattgaag attatacttc cttagtgaaa
    14221 ttatctgatg tagatgaata taatcaagag tttaacgtag aacctggcaa cagtttatct
    14281 aagttttcat aaaaggagtt ttagtattat gaaaatcatt aacacaacaa gattaccgga
    14341 agcacttgga ccatattcgc atgcaacagt tgtgaatggt atggtttata cttctggtca
    14401 gattccattg aatgttgatg gggaaatcgt aagcgctgat gttcaagcac agacaaaaca
    14461 agttttagaa aatttaaagg ttgttttgga agaagcagga tctgatttga attctgttgc
    14521 gaaagcgacc attttcatta aagatatgaa tgatttccaa aaaataaatg aagtgtatgg
    14581 tcaatatttt aatgaacaca agccagcgcg tagttgtgta gaggttgcgc gtttgccaaa
    14641 agatgtgaaa gtagaaattg aattagtaag taaaattaag gaattataat tttcgattaa
    14701 tatgtttaat caagcttcta aataaaacag agagatatat actatagggg ggctcactac
    14761 atgaaagtga cagatgtaag acttagaaaa atacaaacag atggacgaat gaaagcactc
    14821 gtttccatta cattagatga agctttcgta attcatgatt tacgtgtaat tgaaggaaac
    14881 tctggcttgt tcgttgcaat gccaagtaaa cgtacaccag atggtgaatt ccgcgacatc
    14941 gcgcatccta ttaattcaga tatgagacaa gaaattcaag atgcagtgat gaaagtatat
    15001 gatgaaacag atgaagtagt accagataaa aacgctacat cagaagattc agaagaagct
    15061 taatcaattt tatatttagc gatgtaatac atttgcaata agttgatttg atactgtcga
    15121 taaagcataa agctttgtcg gcagtttttt tagtttgtat taatgttttt ttatttttaa
    15181 tgaaaggcta ataaatatat acgttaacag attatgatga tatgaaaatt attgattcat
    15241 tgctgataaa aattgtgttt gaatgatgct cgtatttttg aagtaagaaa aaagttgttt
    15301 ttaaaattac aacgaattaa aaacaatgcc ttttatatgt tgaaagagta ttgcagatta
    15361 aattataata atgacgaagt gtaaaattta atgggggtta atgttcatgc gaagacacgc
    15421 gataattttg gcagcaggta aaggcacaag aatgaaatct aaaaagtata aagtgctaca
    15481 cgaggttgct gggaaaccta tggtcgaaca tgtattggaa agtgtgaaag gctctggtgt
    15541 cgatcaagtt gtaaccatcg taggacatgg tgctgaaagt gtaaaaggac atttaggcga
    15601 gcgttcttta tacagttttc aagaggaaca actcggtact gcgcatgcag tgcaaatggc
    15661 gaaatcacac ttagaagaca aggaaggtac gacgatcgtt gtatgtggtg acacaccgct
    15721 catcacaaag gaaacattag aaacattgat tgcgcatcat gaggatgcta atgctcaagc
    15781 aactgtatta tctgcatcga ttcaacaacc atatggatac ggaagaatcg ttcgaaatgc
    15841 gtcaggtcgt ttagaacgca tagttgaaga gaaagatgca acgcaagctg aaaaggatat
    15901 taatgaaatt agttcaggta tttttgcgtt taataataaa acgttgtttg aaaaattaac
    15961 acaagtgaaa aatgataatg cgcaaggtga atattacctc cctgatgtat tgtcgttaat
    16021 tttaaatgat ggcggcatcg tagaagtcta tcgtaccaat gatgttgaag aaatcatggg
    16081 tgtaaatgat cgtgtaatgc ttagtcaggc tgagaaggcg atgcaacgtc gtacgaatca
    16141 ttatcacatg ctaaatggtg tgacaatcat cgatcctgac agcacttata ttggtccaga
    16201 cgttacaatt ggtagtgata cagtcattga accaggcgta cgaattaatg gtcgtacaga
    16261 aattggcgaa gatgttgtta ttggtcagta ctctgaaatt aacaatagta cgattgaaaa
    16321 tggtgcatgt attcaacagt ctgttgttaa tgatgctagc gtaggagcga atactaaggt
    16381 cggaccgttt gcgcaattga gaccaggcgc gcaattaggt gcagatgtta aggttggaaa
    16441 ttttgtagaa attaaaaaag cagatcttaa agatggtgcc aaggtttcac atttaagtta
    16501 tattggcgat gctgtaattg gtgaacgtac taatattggt tgcggaacga ttacagttaa
    16561 ctatgatggt gaaaataaat ttaaaactat cgtcggcaaa gattcatttg taggttgcaa
    16621 tgttaattta gtagcacctg taacaattgg tgatgatgta ttggtggcag ctggttccac
    16681 aatcacagat gacgtaccaa atgacagttt agctgtggca agagcaagac aaacaacaaa
    16741 agaaggatat aggaaataat catttacgta tttaaaatga ctaggataaa aggataatcc
    16801 tatgtaatat taatgtaatc tttatgattt aatgattcgc atagtaatgg agttacattt
    16861 tatatataat agtaattgcg taagtaaata attggaggac tataaatgtt aaataatgaa
    16921 tataagaatt cgtcattaaa gattttttca ttgaaaggaa acgaagcatt agcgcaagaa
    16981 gttgctgacc aagtaggaat tgaactaggt aaatgttcag ttaaacgttt tagtgatgga
    17041 gaaattcaaa ttaatatcga agagagtatt cgtggttgtg acgtatttat tattcaacca
    17101 acatcatatc ctgtgaatct acatttaatg gaattattaa ttatgattga cgcttgtaaa
    17161 cgtgcttctg cagcaacaat caatattgta gtgccatatt atggatatgc aagacaagat
    17221 agaaaagccc gtagccgtga gccaatcaca gctaaattag ttgcaaactt aatcgaaaca
    17281 gctggcgcaa ctcgtatgat tgcgttagac ttacatgcac cacaaattca aggattcttt
    17341 gatattccaa ttgaccactt aatgggtgtg ccaattcttg ctaaacattt caaagatgat
    17401 ccgaatatta acccagaaga atgtgtcgtt gtttcaccag accatggcgg tgttacacgt
    17461 gcacgtaaat tagctgacat tttaaaaact ccaattgcaa ttatagataa acgtcgtcct
    17521 agaccaaatg ttgctgaagt gatgaacatt gttggtgaga ttgaaggacg tacggcaatt
    17581 attattgacg atattattga tacagcaggt acaatcactt tagctgcaca agcattaaaa
    17641 gataaaggtg ctaaagaagt atatgcttgt tgtacacacc ctgttttatc aggaccggct
    17701 aaagaacgta tcgaaaattc tgctataaaa gaattaatcg taacaaactc aattcattta
    17761 gatgaagacc gcaaaccatc taacactaaa gaattatctg ttgctggttt aatcgcacaa
    17821 gctatcattc gtgtatacga aagagaatca gttagcgtat tatttgacta atatttaaaa
    17881 ggcgtttgac gaacatattc caaacgtgta taatagtttc gttcgtgatt atacgaataa
    17941 ataaacactt gcaagcaacg atgatgttga tgggtaagtg aggtgctcgt tttgagcaaa
    18001 aatgaaaggt ggaaatgaga atggcttcat taaagtcaat catccgtcaa ggtaaacaaa
    18061 cacgttcaga tcttaaacaa ttaagaaaat ctggtaaagt accagcagta gtatacggtt
    18121 acggtactaa aaacgtgtca gttaaagttg atgaagtaga attcatcaaa gttatccgtg
    18181 aagtaggtcg taacggtgtt atcgaattag gcgttggttc taaaactatc aaagttatgg
    18241 ttgcagacta ccaattcgat ccacttaaaa accaaattac tcacattgac ttcttagcaa
    18301 tcaatatgag tgaagaacgt actgttgaag taccagttca attagttggt gaagcagtag
    18361 gcgctaaaga aggcggcgta gttgaacaac cattattcaa cttagaagta actgctactc
    18421 cagacaatat tccagaagca atcgaagtag acattactga attaaacatt aacgacagct
    18481 taactgttgc tgatgttaaa gtaactggcg acttcaaaat cgaaaacgat tcagctgaat
    18541 cagtagtaac agtagttgct ccaactgaag aaccaactga agaagaaatc gaagctatgg
    18601 aaggcgaaca acaaactgaa gaaccagaag ttgttggcga aagcaaagaa gacgaagaaa
    18661 aaactgaaga gtaattttaa tctgttacat taaagttttt atactttgtt taacaagcac
    18721 tgtgcttatt ttaatataag catggtgctt tttgtgttat tataaagctt aattaaactt
    18781 tattgctttg taataaagtt taattaattt tagtgagtaa aagacattaa actcaacaat
    18841 gatacatcat aaaaatttta atgtactcga ttttaaaata catacttact aagctaaaga
    18901 ataatgataa ttgatggcaa tggcggaaaa tagatgttgt cattataata ataaatgaaa
    18961 caattatgtt ggaggtaaac acgcatgaaa tgtattgtag gtctaggtaa tataggtaaa
    19021 cgttttgaac ttacaagaca taatatcggc tttgaagtcg ttgattatat tttagagaaa
    19081 aataattttt cattagataa acaaaagttt aaaggtgcat atacaattga acgaatgaac
    19141 ggcgataaag tgttatttat cgaaccaatg acaatgatga atttgtcagg agaagcagtt
    19201 gcaccgatta tggattatta caatgttaat ccagaagatt taattgtctt atatgatgat
    19261 ttagatttag aacaaggaca agttcgctta agacaaaaag gaagtgcggg cggtcacaat
    19321 ggtatgaaat caattattaa aatgcttggt acagaccaat ttaaacgtat tcgtattggt
    19381 gtgggaagac caacgaatgg tatgacggta cctgattatg ttttacaacg cttttcaaat
    19441 gatgaaatgg taacgatgga aaaagttatc gaacacgcag cacgcgcaat tgaaaagttt
    19501 gttgaaacat cacgatttga ccatgttatg aatgaattta atggtgaagt gaaataatga
    19561 caatattgac aacgcttata aaagaagata atcattttca agaccttaat caggtatttg
    19621 gacaagcaaa cacactagta actggtcttt ccccgtcagc taaagtgacg atgattgctg
    19681 aaaaatatgc acaaagtaat caacagttat tattaattac caataattta taccaagcag
    19741 ataaattaga aacagattta cttcaattta tagatgctga agaattgtat aagtatcctg
    19801 tgcaagatat tatgaccgaa gagttttcaa cacaaagccc tcaactgatg agtgaacgta
    19861 ttagaacttt aactgcgtta gctcaaggta agaaagggtt atttatcgtt cctttaaatg
    19921 gtttgaaaaa gtggttaact cctgttgaaa tgtggcaaaa tcaccaaatg acattgcgtg
    19981 ttggtgagga tatcgatgtg gaccaatttc ttaacaaatt agttaatatg gggtacaaac
    20041 gggaatccgt ggtatcgcat attggtgaat tctcattgcg aggaggtatt atcgatatct
    20101 ttccgctaat tggggaacca atcagaattg agctatttga taccaaaatt gattctattc
    20161 gggattttga tgttgaaacg cagcgttcca aagataatgt tgaagaagtc gatatcacaa
    20221 ctgcaagtga ttatatcatt actgaagaag tgatcagcca tcttaaagaa gagttaaaaa
    20281 ctgcatatga aaatacaaga cccaaaatag ataaatcagt gcgcaatgat ttgaaagaaa
    20341 cgtatgaaag ctttaaatta ttcgaaagta catactttga tcatcaaata ctacgtcgct
    20401 tagtagcgtt tatgtatgaa acaccttcga caattattga gtatttccaa aaagatgcaa
    20461 tcattgcagt tgatgaattt aatcgtatta aagaaactga agaaagttta acagtagagt
    20521 ctgattcgtt tattagcaat attattgaaa gtggtaatgg atttatagga caaagtttta
    20581 taaaatatga tgattttgaa acattgattg aaggctatcc tgtcacttat ttttcattat
    20641 tcgctacaac aatgccgata aaactaaatc atattattaa attttcatgt aaacctgtcc
    20701 aacaatttta tgggcaatat gacattatgc gttctgaatt tcaacgatat gttaatcaaa
    20761 actatcatat cgtggttttg gtcgaaaccg aaactaaagt tgaacgtatg caagcgatgt
    20821 taagtgaaat gcatattcca tcaataacaa aattgcatcg ctcaatgtca tcgggacaag
    20881 cagtgattat tgagggcagt ttatctgaag gatttgaact acctgatatg ggattagttg
    20941 tcattactga gcgtgagctt tttaaatcaa aacagaaaaa gcaacgaaaa cgtacgaaag
    21001 ctatctcaaa tgctgaaaaa attaagtctt accaagattt aaatgtggga gattatattg
    21061 ttcatgtgca tcatggtgtt ggtagatatt taggtgttga gacgctcgaa gtggggcaaa
    21121 cgcatcgtga ttatattaaa ttgcaatata aaggtacgga tcaactattt gttccagtag
    21181 atcaaatgga tcaagttcaa aaatatgtag cttcggaaga taagacgcca aaattaaata
    21241 aactcggtgg cagtgaatgg aaaaaaacaa aagctaaagt tcaacaaagt gttgaagata
    21301 ttgctgaaga gttgattgat ttatataaag aaagagaaat ggcagaaggt tatcaatatg
    21361 gggaagacac agctgagcaa acaacatttg aattagattt tccatatgaa cttacgcctg
    21421 accaagctaa atctatcgat gaaattaaag atgacatgca aaaatcgcgt ccaatggatc
    21481 gcttgctatg tggtgatgtt ggttatggta aaactgaagt tgcagtgaga gcagcattca
    21541 aagctgtaat ggaaggaaag caggttgcat ttttagttcc tacaactatt ttagctcagc
    21601 aacattatga gacgttaatt gagcgtatgc aagattttcc tgttgaaatt caattaataa
    21661 gtcgttttag aacgcctaaa gagataaaac aaactaagga aggacttaaa actggatttg
    21721 ttgacatagt tgttggtaca cacaaattac ttagtaaaga tatacagtat aaagatttag
    21781 ggctgttgat tgtagatgaa gaacaacgat ttggtgtacg ccataaagag cgtattaaaa
    21841 cattaaaaca taatgtagat gtactaacat tgactgcaac cccaatacct agaacattgc
    21901 atatgagtat gctaggtgtg cgcgatttgt cagtgattga aacgccgcca gaaaatcgtt
    21961 tcccagttca aacatatgta ttagaacaga acatgagttt tatcaaagaa gctttagaaa
    22021 gagaactatc ccgtgatggc caagtgtttt atctttataa taaagtgcaa tccatttatg
    22081 aaaaacgaga acaactccag atgttaatgc cagatgctaa cattgcagtt gctcatggac
    22141 aaatgacaga gcgcgattta gaagaaacga tgttaagttt tatcaataat gaatatgata
    22201 ttttagtaac gacgacgatt attgaaacag gtgtcgatgt cccaaatgca aatactttga
    22261 tcattgaaga tgcagatcgc tttggattga gtcagttgta tcaattaaga ggtcgtgttg
    22321 gtcgttcaag tcgtattggt tatgcatact tcttacatcc agcaaataag gtactaactg
    22381 agactgcaga agatcgatta caagcgatta aagaatttac ggagttaggc tcaggattta
    22441 agattgcgat gcgtgatttg aacattcgtg gtgctggtaa tttgttaggt aaacaacagc
    22501 acggctttat tgatacagtt ggatttgatt tgtacagtca aatgttagaa gaagctgtaa
    22561 atgaaaaacg tggtattaag gaaccagaat ctgaggtgcc agaagtcgaa gttgatttaa
    22621 acttggatgc atatttgccg acagaatata ttgcaaatga acaagctaaa attgaaattt
    22681 ataaaaagct acgaaaaact gaaacatttg atcaaattat cgacattaaa gatgaattaa
    22741 ttgatcgttt taatgattat cctgttgaag tagcacgttt gcttgatata gtggaaataa
    22801 aagtacacgc attacattca ggtatcacgt tgattaaaga taaagggaaa ataattgata
    22861 ttcatttatc tgtaaaagcc actgaaaata ttgatggcga agtgctgttc aaagcaacac
    22921 aacctttagg tagaacaatg aaggttggtg ttcaaaataa tgcaatgaca attactttaa
    22981 cgaaacaaaa tcaatggctt gatagtttga agtttttagt taagtgcatt gaagaaagta
    23041 tgagaatcag tgatgaagca taaagaagca tttaatggcg ttgtcgtgtt aactgctgca
    23101 ttaattgtca ttaaaattct gagtgctgta tatcgaattc catatcaaaa tatattaggt
    23161 gatacaggtt tgtatgcata tcaacaagtg tatccaattg tagcattagg aatgatatta
    23221 tcgatgaatg ccattcctag tgcaattaca caaaatatag ggaagtatca tagtgacgaa
    23281 gcatatgcaa aagcggtcgc ttatatacaa ttagttggta tattattatt tattgctatt
    23341 tttgtgtttg cgaacaatat tgcacatatg atgggtgata gccatttaac accaatgatt
    23401 caagcagcaa gtttaagctt tatatttata ggtatgcttg gcgtgttaag aggttattat
    23461 caatctgcaa ataatatgac agttccggct atttcccagg ttatagaaca agttatacga
    23521 gtaggtatta tcattgttac tattgttatt tttgtagaca gaggttggac gatatatgaa
    23581 gcgggaacaa ttgctatttt agcatcaacg ataggttttt taggttcttc aatttattta
    23641 gtagcgcacc gaccttttaa gtttaaaatg gtaaataaca ctgcaaagat tgtttggaaa
    23701 cagttcgcac tttcggtttt gattttcgct atcagtcaat taatcgtaat tttatggcaa
    23761 gtgattgata gtgttactat tattaagtca cttcaagcga tacgcgtgcc attcgatgtt
    23821 gccataactg aaaaaggagt ctatgaccgt ggtgcatcat ttattcagat gggattgatt
    23881 gtaactacaa catttagttt tgcgctcatt cctctgttaa gtgacgcaat caacatgaat
    23941 aatcaggtac ttatgaatcg ttatgcaaat gcgtcattaa agattacgat tttaataagt
    24001 acagcagcgg gaataggatt aattaattta ttgcctttaa tgaacggtgt gttttttaag
    24061 acgaatgatt taaccttaac gttaagtgtt tatatgatta cggtcatttg tgtatcgtta
    24121 attatgatgg atatggcttt attacaagcg caacatgctg tgagacctat ttttgttggt
    24181 atgacggcag gattggttat taaatttata cttaatatca ttttgattcg tttaagtggc
    24241 attattggtg cgagcattag tactgttgta tcattaatta tattcggtac gattatccat
    24301 attgctgtca cgagaaaata ccacttacat gcgatgagac gattttttat caatgttgtt
    24361 ttaggtatgg tatttatgtc gattgttgtt caatgcgtgt taaacatagt gacaacacac
    24421 ggtagattca ctggactcat tgaattatta tgtgcagcag tattaggtat cattgcattg
    24481 tttttctata tttttagatt taatgttttg acatataaag agttaactta tttaccattt
    24541 ggttcaaagt tgtatcaaat taagaaagga agacgttgat ggcacatacc attacgattg
    24601 ttggcttagg aaactatggc attgatgatt tgccgctagg gatatataaa tttttaaaga
    24661 cacaagataa agtttatgca agaacgttag atcatccagt tatagaatca ttgcaagatg
    24721 aattaacatt tcagagtttt gaccatgttt atgaagcaca tgaccaattt gaagatgtct
    24781 ataatgatat tgtggcgcaa ttggttgaag ctgctaatga aaaagatatt gtctatgcgg
    24841 ttccgggtca tcctagagtt gctgagacaa ctacagtgaa attactggct ttagcaaagg
    24901 acaatactga tatagatgtg aaagttttag gtggtaaaag ctttattgat gatgtgtttg
    24961 aagcagttaa tgtagatcca aatgatggct tcacactgtt agatgcgaca tcattacaag
    25021 aagtaacact taatgttaga acgcatacat tgattacaca agtttatagt gcaatggttg
    25081 ctgctaattt gaaaatcact ttaatggaac gatatcctga tgattaccct gttcaaattg
    25141 tcactggtgc acgaagcgat ggtgcggata acgttgtgac atgcccatta tatgaattgg
    25201 atcatgatga aaatgcattc aataatttga cgagtgtatt cgtaccaaaa atcataacat
    25261 cgacatattt gtatcatgac tttgattttg caacggaagt gattgatact ttagttgatg
    25321 aagataaagg ttgtccatgg gataaagtgc aaacgcatga aacgcttaag cgttatttac
    25381 ttgaagaaac atttgaattg ttcgaagcta ttgacaatga agatgattgg catatgattg
    25441 aagagctagg agatatttta ttacaagtgt tattgcatac tagtattggt aaaaaagaag
    25501 ggtatatcga cattaaagaa gtgattacaa gtcttaatgc taaaatgatt cgtagacacc
    25561 cacacatatt tggtgatgcc aatgctgaaa ctatcgatga cttaaaagaa atttggtcta
    25621 aggcgaaaga tgctgaaggt aaacagccaa gagttaaatt tgaaaaagta tttgcagagc
    25681 attttttaaa tttatatgag aagacgaagg ataagtcatt tgatgaggcc tcgttaaagc
    25741 agtggctaga aaaaggggag agtaatacat gagattagat aaatatttaa aagtatcacg
    25801 gttaataaag cgacgtacgc tagcaaaaga agtaagtgat caaggtagaa ttacaataaa
    25861 tggtaatgtt gctaaagctg gatcggatgt taaagttgaa gatgtgctga cgattcgctt
    25921 tggtcaaaaa ttagtaacag ttaaagtaac tgcattaaat gaacatgcat ctaaagataa
    25981 cgcgaagggc atgtatgaaa tcattgaaga gcgtcgactt gaagaagcgt aaattggagg
    26041 tgacaagcaa tgaaaaataa agtagaacat atagaaaatc agtacacgtc gcaagagaat
    26101 aagaaaaaac aacgtcaaaa aatgaaaatg cgtgttgttc gtaggcgtat tacagtattt
    26161 gcgggcgtat tacttgcgat aattgttgtt ttatcaatct tgcttgttgt ccaaaaacat
    26221 cgcaatgata tcgatgcaca ggagcgaaaa gcgaaagaag cacagtttca aaagcaacaa
    26281 aatgaagaaa ttgcgttaaa agaaaagttg aataatctga atgacaaaga ttatattgaa
    26341 aaaattgcgc gtgatgatta ttacttaagc aacaaaggtg aagtgatttt taggttgcca
    26401 gaagacaaag attcgtctag ctcaaaatct tcgaaaaaat aaatccaaat tgattcaaaa
    26461 ttatccgagt atagacattg tgaaaaaatc caaacaagga tataataagg gaaaatcgaa
    26521 tcaaatcggg aggatttatt taacatatgt caatcgaagt tggaaataag cttaaaggta
    26581 aagtcactgg tattaaaaag tttggtgcat tcgtagaatt acctgaagga aaaagtggtt
    26641 tagttcacat tagtgaagtc gcagataatt atgttgaaaa cgtagaagag cacctttctg
    26701 ttggtgatga agtagacgta aaagtattat ctattgctga tgatggaaaa attagtcttt
    26761 caattaagaa agctaaagac cgtccacgta gacaacatac gagtaaacca agtcatcaaa
    26821 aaccagtgca aaaagccgaa gattttgaaa agaaattaag caatttctta aaagatagtg
    26881 aagataaatt aacttcaatc aaacgtcaaa cagaatctag acgcggtggc aaaggttcaa
    26941 gacgttaatt aaaataaata aagactgttt cgataaggaa tatatttaga atgatgcgta
    27001 tcgaataatc gattgcagcg ttagacaatc taagactgtt tcttaaataa ggagcagtct
    27061 cttttatttg taatgatata actaagactt ataccatttt tgaaaattgt aaaagtgagg
    27121 tgatgttatg cagttaaata gtaatggttg gcatgttgat gaccatattg ttgtcgctgt
    27181 ttctacaggt attgatagta tgtgtttatt gtatcaacta ctaaatgatt ataaagatag
    27241 ttatagaaaa ctaacatgct tacatgtcaa tcatggcgtt aggtcagctt caatcgagga
    27301 agccagattt ttagaagtat actgcgaacg tcatcacatc gatttacata tcaaaaagtt
    27361 agatttgtcg catagtctcg accgaaataa cagcattcag aatgaagctc gaattaaacg
    27421 ttacgaatgg tttgatgaaa tgatgaatgt attagaagcg gatgtattgc taacggcgca
    27481 tcatttggac gatcaattag aaactattat gtatcgtatt tttaatggga aatcaacgcg
    27541 taataaacta ggatttgatg agttatcgaa gcgaaaaggt tatcagattt atcgaccact
    27601 tttagctgtc tctaaaaaag aaataaaaca attccaagag agatatcata ttccatattt
    27661 tgaagatgaa tctaataaag ataacaaata tgttagaaat gatattcgta atagaattat
    27721 tccagctatt gatgaaaata atcaacttaa agcatcgcat ttattaaaat taaaacaatg
    27781 gcatgatgaa caatatgata ttttgcaata ttcagctaaa caatttattc aagaatttgt
    27841 gaagtttgat gaacagtcaa aatatttaga ggtttctaga caagctttta ataacttacc
    27901 aaactcatta aagatggttg tgttggattg cctactatca aagtattatg agttgtttaa
    27961 tattagtgct aaaacatacg aagagtggtt taaacaattt agtagtaaga aagcacaatt
    28021 cagtattaat ctcacggata aatggataat tcaaattgca tatggtaaat taataataat
    28081 ggctaaaaat aatggcgata catattttag agttcaaact attgaaaagc caggtaatta
    28141 tatttttaac aaatatcgat tagagataca ttctaattta ccaaaatgtt tatttccgct
    28201 tacagtgaga acacgacaaa gtggcgatac atttaaactg aatgggcgcg atggttataa
    28261 gaaagtgaat cgcctgttta tagattgtaa agtgcaacag tgggttcggg atcaaatgcc
    28321 aatcgtattg gataaacaac agcgcattat tgcggtagga gatttatatc aacaacaaac
    28381 aataaaacaa tggattataa ttagtaaaaa tggagatgaa tagcgttatg cataatgatt
    28441 tgaaagaagt attgttaact gaagaagata ttcaaaatat ctgtaaggaa ttgggagcac
    28501 aattaacaaa ggattatcaa ggtaaaccat tagtatgcgt gggtatctta aaaggctcag
    28561 caatgtttat gtcagattta attaaacgaa ttgataccca tttatcaatt gatttcatgg
    28621 atgtttctag ttatcacgga ggcactgagt caactggtga agttcaaatc attaaagatt
    28681 taggttcttc tattgaaaat aaagacgtat taattattga agatatctta gagactggta
    28741 ctacacttaa gtcaattact gaattattac aatctagaaa agttaattca ttagaaatag
    28801 ttactttatt agataaacca aaccgtcgta aagcggacat tgaagctaag tatgtaggta
    28861 aaaaaatacc agatgaattt gttgttggtt acggtttaga ttatcgtgaa ttataccgaa
    28921 acttaccata tatcggtacg ttaaaacctg aagtgtattc aaattaattt tttaatcaat
    28981 ttcagttatt attactatgc gtttgagaaa taatagtgta gactcaaaaa tatgaa
//
LOCUS       DBNENW010000014        28513 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNENW010000014 DBNENW010000000
VERSION     DBNENW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.014"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            89..6616
                     /gene="sasC"
                     /locus_tag="AC1LW8_000515"
     CDS             89..6616
                     /gene="sasC"
                     /locus_tag="AC1LW8_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored aggregation protein SasC"
                     /protein_id="HIP4772380.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVASNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEPASNRPKKRSRRAAPADPNATPADPGAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNSIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVLSHAMVRTSMFEAGSNRTYQAQGNVLALGRISGTDASNHGDFNGIEKSLTVN
                     PNSELIFEFNTMPTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNSGASLDTDEFVYKIQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTTNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKKRAQT
                     ILAENRNNVPLNKRVSQADIDTLTNQMQHTLIRSVDAENAVNQKADQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKGNIIGDIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDAINQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGSVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATTNAD
                     VDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDATQEERQAAIDKV
                     NAAVTAANTNILNANTNAEVEQVKTNAIQGIQAITPATKVKTDAKNAIDKSAETQHNT
                     IFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGTQNIVVIQPATQ
                     VKTDARNVVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALTDIGVTSTTAMV
                     NSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATTEEKQVALNQVD
                     QELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQINQHYNAKLAEI
                     NATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAENNIDAVQVDVVK
                     KQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAFNQINQNQTNDQVD
                     TTTNQALNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDNEKEVASQALAK
                     EKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKINQKANELRAKIN
                     QDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDSIALVAPEHIVR
                     AAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNKKLALQNINQAVTNNDVKR
                     VETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDELDEANQLISDT
                     LKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEENNKNQLDAIRN
                     TLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNIKVILPKVQVKP
                     AARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQINHADKTAQVNQD
                     SIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQNIAIAQVEKEL
                     IKAKQQIASAVTNADVAYLLHDEKNEIREIEPVISRKASAREQLTTLFNDKKQAIEAN
                     IQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIHDLDVHPIKKPD
                     AEKTINDDLARVTALVQNYRKVSDRNKADALKAITALKLQMDEELKTARTNADVDAVL
                     KRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKVKVLIDQYVADG
                     NRMIDEDATLNDIKQHTQFIVDEILAIKLPAEAMKVSPKVIQPAPKVCTPIKKEETHE
                     SRKVEKELPNTGSEEMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6733..8001)
                     /locus_tag="AC1LW8_000516"
     CDS             complement(6733..8001)
                     /locus_tag="AC1LW8_000516"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP4772381.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8408..10069
                     /locus_tag="AC1LW8_000517"
     CDS             8408..10069
                     /locus_tag="AC1LW8_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIP4772382.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPFITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10066..10761
                     /locus_tag="AC1LW8_000518"
     CDS             10066..10761
                     /locus_tag="AC1LW8_000518"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP4772383.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKDYISATEDHHSKTVIDLIPEYQHLNIFTVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10778..11200
                     /locus_tag="AC1LW8_000519"
     CDS             10778..11200
                     /locus_tag="AC1LW8_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIP4772384.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            11892..13301
                     /gene="sapep"
                     /locus_tag="AC1LW8_000520"
     CDS             11892..13301
                     /gene="sapep"
                     /locus_tag="AC1LW8_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HIP4772385.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFDSDFGEKMGMKFHTDVMGDVTNNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            13305..14153
                     /gene="dat"
                     /locus_tag="AC1LW8_000521"
     CDS             13305..14153
                     /gene="dat"
                     /locus_tag="AC1LW8_000521"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047810 - D-alanine-2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HIP4772386.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            14704..15495
                     /locus_tag="AC1LW8_000522"
     CDS             14704..15495
                     /locus_tag="AC1LW8_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIP4772387.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNQVMNSNMFI"
     gene            15510..16154
                     /gene="trmB"
                     /locus_tag="AC1LW8_000523"
     CDS             15510..16154
                     /gene="trmB"
                     /locus_tag="AC1LW8_000523"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIP4772388.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMSLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSNKGSRIYRMEAKFHSQK"
     gene            16624..17466
                     /locus_tag="AC1LW8_000524"
     CDS             16624..17466
                     /locus_tag="AC1LW8_000524"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtnP family quorum-quenching lactonase"
                     /protein_id="HIP4772389.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIITDLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(17591..17902)
                     /locus_tag="AC1LW8_000525"
     CDS             complement(17591..17902)
                     /locus_tag="AC1LW8_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIP4772390.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQYINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            17989..19065
                     /locus_tag="AC1LW8_000526"
     CDS             17989..19065
                     /locus_tag="AC1LW8_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIP4772391.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGSQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            19130..19441
                     /locus_tag="AC1LW8_000527"
     CDS             19130..19441
                     /locus_tag="AC1LW8_000527"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP4772392.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            19541..20398
                     /locus_tag="AC1LW8_000528"
     CDS             19541..20398
                     /locus_tag="AC1LW8_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HIP4772393.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            20427..21023
                     /gene="ytpR"
                     /locus_tag="AC1LW8_000529"
     CDS             20427..21023
                     /gene="ytpR"
                     /locus_tag="AC1LW8_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIP4772394.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            21044..24868
                     /locus_tag="AC1LW8_000530"
     CDS             21044..24868
                     /locus_tag="AC1LW8_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIP4772395.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEADEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRFDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLEDEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            24892..26205
                     /gene="murC"
                     /locus_tag="AC1LW8_000531"
     CDS             24892..26205
                     /gene="murC"
                     /locus_tag="AC1LW8_000531"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIP4772396.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            26279..26770
                     /locus_tag="AC1LW8_000532"
     CDS             26279..26770
                     /locus_tag="AC1LW8_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIP4772397.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            26844..28208
                     /locus_tag="AC1LW8_000533"
     CDS             26844..28208
                     /locus_tag="AC1LW8_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772398.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEVSANNLSDTSQEAQEIQEAKKEAQAETDKSTAVSNEESKAAALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEESKAAALKAQQAAIKEEAS
                     ANNLSDTSQEAQEIQEAKREAQAETDKSVAVSNEESKAVALKVSKEESQAERLANAAK
                     QKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSESETVNIDNKDTVK
                     QKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQ
                     HNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANNKIEKR
                     TFND"
     gene            28364..28447
                     /locus_tag="AC1LW8_000534"
     CDS             28364..28447
                     /locus_tag="AC1LW8_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772399.1"
                     /translation="MLFVTSLDYNSDIAHFKLKMEKYSIIK"
BASE COUNT        10852 a         4176 c         5239 g         8246 t
ORIGIN      
        1 tagctataaa cataatgtga gggagcgtat atttttagca taattcttaa caacacagca
       61 gagaacagac aaccaggagg aaaatgaaat gaatttgtta aagaaaaata aatatagtat
      121 taggaagtat aaagtaggca tattctctac tttaatcgga acagttttat tactttcaaa
      181 cccaaatggt gcacaagcct taactacgga taataatgta caaagcgata ctaatcaagc
      241 aacacctgta aattcacaag ataaagatgt tgctaataat agaggtttag caaatagtgc
      301 gcagaataca cctaatcaat ctgcaacaac caatcaagca acgaatcaag cattggttaa
      361 tcataataat ggtagtatag taaatcaagc tacgccaaca tcagtgcaat caagtacgcc
      421 ttcagcacaa aacaataatc atacagatgg caatacaaca gcaactgaga cagtgtcaaa
      481 cgctaataat aatgatgtag cgtcgaataa taccacatta aatgtaccaa ataaaacaaa
      541 tgaaaatggt tcaggaggac atctaacttt aaaggaaatt caagaagatg ttcgtcattc
      601 ttcagataaa ccagagctag ttgcaattgc tgaaccagca tctaatagac cgaaaaagag
      661 aagtagacgt gcggcaccgg cagatcctaa tgcaactcca gcagatccag gggctgcagc
      721 ggcaggaaac ggtggtgcac cagttgcaat tacagcgcca tacacgccta caactgatcc
      781 taatgccaat aatgcaggac aaaatgcacc taacgaagtg ctgtcatttg atgacaatag
      841 tattagacca agtaccaacc gttctgtacc atcagtaaca gttgttgata atttaccagg
      901 ctttacactt atcaatggtg gtaaagtagg tgtgctaagt catgcgatgg ttagaacaag
      961 tatgtttgag gctgggagca acagaaccta tcaggcacaa ggcaatgtac ttgcattagg
     1021 acgtataagc gggacggacg ctagtaatca tggagatttt aatggtattg aaaaatcttt
     1081 aacagtaaat ccaaattctg aattaatctt tgaatttaat acaatgccaa ctaaaaatgg
     1141 tcaaggcgca acaaatgtta ttatcaaaaa tgctgatact aatgatacga ttgctgaaaa
     1201 gactgttgaa ggcggtccaa ctttgcgttt atttaaagta cctgataatg tgagaaatct
     1261 caaaattcaa tttgtaccta aaaatgacgc aataacagat gcgcgtggca tttatcaact
     1321 aaaagatggt tacaaatact atagctttgt tgactctatc ggacttcatt ctgggtcaca
     1381 tgtttatgtt gaaagacgaa caatggaacc cacagcaacg aataataaag aatttacagt
     1441 tacaacatca ctaaaaaata atggtaattc tggtgcttct ttagacacag atgaatttgt
     1501 ttataaaatt caattacctg aaggggtaga atatgtgaac aattcattga ctaaagattt
     1561 tccaagtaac aattcaggcg ttgatgttaa tgatatgaat gttacatatg atgcggcgaa
     1621 tcgagtgata acaattaaaa gcactggtgg tggcacaacg aattcacctg cacgactaat
     1681 gcctgataaa atattggatt taaagtataa gttgcgtgtt aataatgtac cgacaccaag
     1741 aacagtaaca tttaatgata cattaacgta taaaacatat acacaagatt tcattaattc
     1801 agctgcagaa agtcatactg taagtacaaa tccatatact atcgatatca tcatgaataa
     1861 agatgcatta caagccgaag ttgacagacg tattcaacaa gctgattata catttgcatc
     1921 attagatatc tttaatgatc ttaaaaaacg tgcacaaaca attttagctg aaaatcgtaa
     1981 caatgtacca ttaaataaaa gagtatctca agcagatatt gatacattaa ctaatcagat
     2041 gcaacatact ttaattcgaa gtgttgatgc tgaaaatgca gtaaatcaaa aagctgatca
     2101 aatggaagat ttagttaatc aaaatgatga actgacagat gaagaaaaac aagcagcaat
     2161 acaagttatc gaggaacata aaggcaatat tattggtgat attggtgatc aaacgactga
     2221 tgatggcgtt actagaatca aagatcaagg tatacagacc ttaagtgggg atactgcaac
     2281 accggttgtt aaaccaaatg ctaaaaaagc aatacgtgat aaagcaacga aacaaaggga
     2341 aattatcaat gcaacaccag atgctactga agacgagatt caagatgcaa taaatcaatt
     2401 agctacggat gaaacagatg ctattgataa tgttacgaat gctactacaa atgctgacgt
     2461 tgaaacagct aaaaataatg gcatcaatac tattggatca gttgttcctc aagtgacaca
     2521 taaacaagct gcaagagatg caattaacca agcaacagca acgaaaagac aacaaataaa
     2581 tagtaataga gaagcaactc aggaagagaa aaatgcagca ttgaacgaat taactcaagc
     2641 aacaacaaat gctgatgttg ataacgccaa aggagatggt ctaaatgcca ttaatccaat
     2701 tgctcctgta actgttgtta agcaagctgc aagggatgcc gtatcacatg atgcacaaca
     2761 acatatcgca gagatcaatg ctaatcctga tgcgactcaa gaagaaagac aagcagcaat
     2821 tgacaaagtg aatgctgctg taactgcagc aaacacaaac attttaaacg ctaataccaa
     2881 tgctgaagtg gaacaagtga agacaaatgc gatacaagga atacaagcaa ttacaccagc
     2941 tacaaaggta aaaacagatg caaaaaatgc catcgataaa agtgcggaaa cgcaacataa
     3001 tacgatattt aataataatg atgcgacgct cgaagaacaa caagcagcac aacaattact
     3061 tgatcaagct gtagccacag cgaagcaaaa tattaatgca gcagatacga atcaagaagt
     3121 tgcacaagca aaagatcagg gcacacaaaa tatagtagtg attcaaccgg caacacaagt
     3181 taaaacggat gctcgcaatg ttgtaaatga taaagcgcga gaggcgataa caaatatcaa
     3241 tgctacacct ggcgcgactc gagaagagaa acaagaagcg ataaatcgtg tcaatacact
     3301 taaaaataga gcattaactg atattggtgt gacatctact actgcgatgg tcaatagtat
     3361 tagagacgat gcagtcaatc aaatcggcgc agttcaaccg catgtaacga agaaacaaac
     3421 tgctacaggt gtattaaatg atttagcaac tgctaaaaag caagaaatta atcaaaacac
     3481 aaatgcaaca actgaagaaa agcaagtggc tttaaatcaa gtggatcaag agttagcaac
     3541 ggcaattaat aatataaatc aagctgatac aaatgcggaa gtagatcaag cgcaacaatt
     3601 aggtacaaaa gcaattaatg cgattcagcc aaatattgtt aaaaaacctg cagcattagc
     3661 acaaatcaat cagcattata atgctaaatt agctgaaatc aatgctacac cagatgcaac
     3721 gaatgatgag aaaaatgctg cgatcaatac tttaaatcaa gacagacaac aagctattga
     3781 aagtattaaa caagctaaca caaatgcaga agtagaccaa gctgcgacag tagcagagaa
     3841 taatatcgat gctgttcaag ttgacgtagt aaaaaaacaa gcagcgcgag ataaaatcac
     3901 tgctgaagtg gcgaagcgta ttgaagcggt taaacaaaca cctaatgcaa ctgacgaaga
     3961 aaagcaggct gctgttaatc aaatcaatca acttaaagat caagcattta atcaaattaa
     4021 tcaaaaccaa acaaatgatc aggtagacac aactacaaat caagcgttaa atgctataga
     4081 taatgttgaa gctgaagtag taattaaacc aaaggcaatt gcagatattg aaaaagctgt
     4141 taaagaaaag caacagcaaa ttgataatag tcttgattca acagataatg agaaagaagt
     4201 tgcttcacaa gcattagcta aagaaaaaga aaaagcactt gcagctattg accaagctca
     4261 aacgaatagt caggtgaatc aagcagcaac aaatggtgta tcagcgatta aaattattca
     4321 acctgaaaca aaagttaaac cagctgcacg tgaaaaaatc aatcaaaaag cgaatgaatt
     4381 acgtgctaag attaatcagg ataaagaagc aacagcagaa gaaagacaag tagcactaga
     4441 taaaatcaat gaatttgtaa atcaagccat gacagatatt acgaataata gaacaaatca
     4501 acaagttgat gatacaacaa gtcaagcgct tgatagcatt gctttagtag cgcctgaaca
     4561 tattgttaga gcagctgcta gagatgcagt aaagcaacaa tatgaggcta aaaagcaaga
     4621 aatagagcaa gctgaacatg cgactgatga agaaaaacaa gttgctttaa atcaattagc
     4681 gaataataaa aaacttgcat tacaaaacat caatcaagca gtaacgaata atgatgtgaa
     4741 acgtgttgaa acaaatggca ttgctacact aaaaggtgta caacctcata ttgtaattaa
     4801 gcctgaagca caacaagcaa taaaagcaag tgcagaaaat caagtagaat caataaaaga
     4861 tacaccacat gcaacagttg atgaattaga tgaagcgaat caattaatta gcgacacact
     4921 caaacaagcg caacaagaaa tagaaaatac aaatcaagat gctgctgtta ctgatgttag
     4981 aaatcaaaca atcaaggcaa ttgagcaaat aaaacctaaa gtaagacgta aacgagctgc
     5041 gcttgatagc attgaagaaa ataataaaaa tcaactcgat gcaatccgaa atacgttgga
     5101 tactactcaa gatgaaagag atgttgctat tgatacttta aataaaattg taaatacaat
     5161 taaaaatgac attgcacaaa acaaaacgaa tgcagaagtg gatcgaactg agactgatgg
     5221 caacgacaac atcaaagtga ttttacctaa agttcaagtt aaaccagcag cgcgtcaatc
     5281 tgttggtgta aaagccgaag ctcaaaatgc actaatcgat caaagcgatt tatcaactga
     5341 agaagaaaga ctagctgcta aacatttagt agaacaagca cttaatcagg ctattgatca
     5401 gatcaatcat gcagataaga ctgcccaagt taatcaagat agtatagatg ctcaaaatat
     5461 tatttcaaaa attaaaccag cgacaacagt taaagcaaca gcattacaac aaattcaaaa
     5521 tatcgctaca aataaaatta atttaattaa agcaaataac gaagcgacag atgaagaaca
     5581 aaatattgca atagcacaag ttgaaaaaga gttaattaaa gctaaacaac aaattgctag
     5641 tgcagtgact aatgctgatg tggcatattt attgcatgat gagaaaaacg aaattcgtga
     5701 aatcgaacct gttattagca gaaaggcgtc tgctcgagaa caattgacaa cattattcaa
     5761 cgataaaaaa caagcaattg aagcgaatat tcaagcaacg gtagaagaaa gaaatagtat
     5821 attagcacag ttacaaaata tttatgacac tgctattgga caaattgatc aagatcgtag
     5881 caatgcacaa gttgataaaa cagcatcatt aaatctacaa acaatacatg atttagatgt
     5941 acatcctatt aaaaagccag atgctgaaaa aacgattaat gatgatcttg cacgcgtcac
     6001 tgctttagtg caaaattatc gaaaagtaag tgatcgtaat aaggctgatg cattaaaagc
     6061 tataactgct ttaaaattac aaatggatga agaattaaaa acagcacgca ctaatgctga
     6121 tgttgatgca gttttaaaac gatttaatgt tgcattaagc gatatagaag cagtaattac
     6181 tgaaaaagaa aatagcttac tgcgcattga taacattgct caacaaacat atgcgaaatt
     6241 caaagcgatc gcaacaccag aacaattagc taaagtaaaa gtattaattg atcaatatgt
     6301 tgcagatggc aatagaatga ttgatgaaga tgcgacatta aatgacatca aacaacatac
     6361 gcaattcatt gttgatgaaa ttttagcaat taaattacca gctgaagcga tgaaagtatc
     6421 accaaaagta attcagccag ctccaaaagt ttgtacgcct attaaaaaag aagagacaca
     6481 tgaatcgcgc aaagttgaaa aagaacttcc aaatacaggt tctgaagaaa tggatttacc
     6541 attgaaagaa tttgcactga ttacaggtgc ggctttgtta gctagaagac gtactaaaaa
     6601 cgaaaaagaa tcataattaa cgaaatgacc ttaatataac gacacttttt agtgactcag
     6661 ttatattaag gtcattttta attgaatgaa aaagttgatc tactaaagag aatcgaacca
     6721 aattttatac tattattcca ttgatgcatg tgagtaatgt ccggcatata atccagcgac
     6781 atgtcctgtt acgagtgcac ttgtaatatt ataaccacca gtataaccat gtatatctaa
     6841 tacttcacca cataaaaata atcccggaac taatttagac atcattgttt taggttgaat
     6901 ttctttaagt gacacaccac cacctgtgac aaatgcctta tctataggta atgtcccgtt
     6961 caccttaaat acaaaccctt taaacatatt tactaagtcg ttcaattgtt gatttgataa
     7021 gtgatgtgat gtggtatttt catcgattcc tgcttgttcc agcatgaaca gtaagtagcg
     7081 ctcttcaatt agaccatgca aactgttttt aatgatttta tctggtgtgt ccgataataa
     7141 tgatgtgatg tgttgtttta attgttcatg gtttaattca ggaaatgcat cgattgccat
     7201 agaaatgtgc tgtgtctttt gatttttttg ttctttataa acaaactgac tacatcttaa
     7261 tgcagctgga ccactgatac caaaatgagt aaataacata tccatttgat gactgatgcg
     7321 ttttttacca tttttcttaa gtactgacaa ttcaacatct tttaaactta aaccttttag
     7381 acgattggat ttgatgaaag gttcagctga tgtaattgga acttcggtcg ggaataactc
     7441 agtaatggta tgacctaaat cttgtgcgaa cttataacca tcaccagttg aaccagtttg
     7501 agggacactt gtaccacctg tagcaatcac tagcgaatgg ctttcataac tattattttg
     7561 agtatgtaca gtgaaagttt ggtctgtatt aacttcgatt ctactaacag cttcttcttc
     7621 tttaatcgtt acatgttggc gttcgatagt tgtcactaat gtatcaacca cgtcttgtgc
     7681 tttgttggaa actggaaaca tacgcccgtg atcttcttct tttaatttaa cacccctaga
     7741 ctcaaaaaaa tctatgatgg attcattatc aaaaattgaa aagggactat ataaaaattt
     7801 cccatttcca ggaatgttct taataatttc agcatatggt aatcgattag ttacgttaca
     7861 tctaccgcca ccagatattt tgagtttacg acctagacct ttcttttttt caatgagtaa
     7921 cacactgcta ctttgttcgc ttgcagctac tgccgccatt aagccgctag gtccgcctcc
     7981 gataataatt gtttgataca tatacgcttc ctccaaattt gttactggac attcaattgc
     8041 tattttatag tactttttta aactttgcta tatctcttgt gttatgagat gacaattatg
     8101 ttgaatatat ttataatgtg aacttcataa aaaatgaaga tgtatgtgta tgataagaat
     8161 gatttttcta ctgttatgcg atgttcatct caaatagtag tataatgttt atatgaaatt
     8221 tcgtaatatt ctaatcaaaa tctagtattc aatgacaatg tgttgataat tttgttatga
     8281 taattcattg aatatacgac aatgaaataa agttttgtga tatattcata ataatgaaaa
     8341 atgattttac agagaaatag cttttagttt acaaaacttt aataaacatg agatagggag
     8401 attcgtaatg agtgaaagta aagaaatggt gcgtggaacc tttttaatta ccattagtat
     8461 attaattacc aaggtattgg gcgtactttt tatcattcca ttcaactatt taattggtgg
     8521 acaagaaaat atggcgccgt tcacatacgc ttatgcacca tataatattg caattgcagt
     8581 tgcgacagca ggtgtaccat tagcagcttc gaaatatgtt gcgaaatata atgctattgg
     8641 ggcatataaa gtcagtcaga aattttataa atcgagtttt attgtaatga gtattaccgg
     8701 tgttttaggt ttcttggtac tgtatttcct agcaccttat atttctgaat taacattagc
     8761 tagaaacgtt catgataaaa acggttggtc agtcgatgat attacttgga ttataagaat
     8821 cattagtatg gttgttatct ttattcctgt attagcgaca tggagaggta tattccaagg
     8881 ttataaatct atggggccaa cagcagtgtc agaagtaact gagcaaattg cacgcgtgat
     8941 tttcatatta atcggaagtt atttagtact aaatgtcttt gatggctcta ttttattagc
     9001 caatggtatt gctacttttg cagcagcagt tggtgcgatt attggtatat tcacactctg
     9061 gtattactgg agaaagcgta agcataatat tgatcgtatg gtcgaatctg attacacaga
     9121 tatagatgtg tcatatggca agatgtataa agaaattatt gcttatagta ttccgttcgt
     9181 tattgtaagt ttgaattatc cattatttaa cttagtagat caatttacac ataacggtgc
     9241 tttatcttta gttggtatac cttcacagtt acaagatata ttctttaata tgctaaatat
     9301 gtcaactaat aaaattgtaa tgattccgac atcattaagt gctggttttg cagtgagttt
     9361 aattcctttt attacaaaaa catttgcaga aggtcgctta catgaaatgc atcatcaaat
     9421 tagaacatct attggtgtat taatgtttat aactgttcca gctagtattg gaattatggc
     9481 cttggcacaa ccattattca cagttttcta tggttacgat ccaattgttt taggtcatga
     9541 ccctaaccat gatggtagtc gcttattatt ctattatgcg cctgtagcaa tactaatttc
     9601 actattaagt gtaacggcat caatgttaca aggtatagat aaacagaaat taacagttta
     9661 tgttatttta gcttctgtag ttatcaagtt agcgttaaac tatccattaa ttatgttatt
     9721 ccatacacct ggtgcaattt taagtactag tatcgcatta ttatttgcaa ttggatgtaa
     9781 tttctatatt cttaaaaaat atgcgaagtt taaattcagc tatagttgga ttcattttgc
     9841 aaaaatattc ttgtattcat ttattatgat gctaggtgta gaattagtat tcttccttgc
     9901 aaatctattc ttagaaccta ctaaacttgg ttacttaatc attattattt taggtgttac
     9961 agtaggtatt ctaatttacg gtacaatcac aattaaaacg cgtcttgcgg acgaattctt
    10021 aggtgaaatt cctgaaaaat taagacgtag agttaggttt ttacgatgag aatagataaa
    10081 tttttagcaa atatgggcgt cggtacacgt aatgaggtca aacagttact taaaaaaggc
    10141 cttgtaaatg tcaacgaaca agtaattaaa tcaccaaaaa cacatataga accagaaaac
    10201 gataagataa cagttcgtgg agaattaata gaatacattg aaaatgtcta tattatgtta
    10261 aataagccaa aagattatat atcagcaact gaagatcatc attcaaaaac cgttattgat
    10321 ttaatacctg aataccaaca tctaaatatt tttacggttg gtcgtcttga taaagataca
    10381 gaagggcttt tattgataac gaatgatggt gattttaatc atgaattaat gagtccaaac
    10441 aaacatgttt ctaaaaaata tgaagttatt tcagcaaatc ctatcactga agacgacatt
    10501 caagctttta aagagggtgt aacactgtct gatggcaagg ttaagcctgc aattttaact
    10561 tatattgata atcaaacgtc acacgtaaca atttatgaag gaaaatatca tcaagttaaa
    10621 cgaatgtttc atagcattca aaatgaggta ttacatttaa gacgtatcaa aattgctgat
    10681 ttagaattgg acagcaatct agattcagga gaatatcgtt tattaactga aaatgatttt
    10741 gacaaattaa attataaata aaagggagac gattgatatg gcaaaaggta atttatttaa
    10801 agcgattttg ggtataggtg gcgctgtagc agctgtactt gttacacgta aagatagtcg
    10861 tgacaagctg aaagcagaat ataataaata taaacaagat cctcaaagct ataaagataa
    10921 tgctaaggat aaagcgacgc aattaggaac aattgctaat gaaacaatta aagaagtaaa
    10981 aacaaatccg aaagaatatg ctaatagatt aaaaaataat ccaaaagcat ttttcgaaga
    11041 agaaaaatca aaatttaccg aatatgacaa taagactgac gaaagtattg aaaaaggtaa
    11101 atttgatgat gaaggtggcg ccgcaccaaa taataattta cgtatcgtca ctgaagaaga
    11161 cttaaaaaag aataaaaatg cactatctga taaagaataa gatgtgagta ggacagaaat
    11221 gataagggag tgggacagaa atcatatttt agcaaaattt atttcgtcgt cccaccccgg
    11281 caaggttgac tagaattgaa aaaagcttgt tacaagcgta ttttcattca gtcaactact
    11341 gccaatataa tattgtaaac tataggacat ttattagtgt ttcaggttct ttatcgttag
    11401 tcatagctaa tatgcatata gaaagaagaa tacatgcgtg aaactcattc ataaaaaata
    11461 ctaatatcta aagaaaacgt atttctttat cttttcgaaa ttctctatgt tggggccctt
    11521 accccaactt gcacattatt gaaagctgac tgttggccag tttctgtgtt ggggcctcgc
    11581 caacttgcat tgtctgtaga atttcttttc gaaattctct atgttggggg ccccggggcg
    11641 cattttcgtt cggttaatta ctgtcaatat taaattgtgt gcctagtaca ttgacttata
    11701 tctcgagttc ttgaatatgt gtattttaaa tatataaatt aaaaaaggaa caaagcagct
    11761 atttatgatt tgtgtcatat cgatagcatc gttttgttcc tttttattat tttctgacat
    11821 cttattgtag tttttatatc atttcatgta aaatagaatt taacttatag aaaaaaggaa
    11881 gtcgatttcg aatgtggaaa gaaaaagttc aacaatacga agatcaaatc attaatgact
    11941 taaaaggatt attagcaatt gaaagtgtga gagatgatgc aaaagcatca gaagacgcac
    12001 cagttggtcc aggtcctcgt aaggcattag actacatgta tgaaattgca catagagatg
    12061 gatttacaac acatgatgtg gatcatattg caggaagaat tgaggcaggt aaaggaaatg
    12121 acgtattagg tatcttatgt catgttgacg ttgttcctgc tggtgatgga tgggatagta
    12181 atccgttcga gccggttgta acagaagatg ctatcatagc tagaggtaca cttgatgaca
    12241 aaggtccaac aattgctgct tattatgcaa ttaagatatt agaagatatg aatgtggatt
    12301 ggaagaaacg tattcatatg attattggta cggatgaaga atctgattgg aaatgtacgg
    12361 atcgctattt taaaacagaa gaaatgccaa cattaggttt tgcaccagat gcagaatttc
    12421 cgtgtattca tggtgaaaaa ggcattacaa catttgactt agttcaaaat aagcttgctg
    12481 aagatcaaga tgaacctgat tatgaattaa taacttttaa atctggtgaa cgttacaaca
    12541 tggtgcctga tcatgcagaa gcaagagtgc ttgttaaaga aaatatgaca gatgttattc
    12601 aagactttga gtacttttta gaacaaaatc atttacaagg tgatagtact gttgatagtg
    12661 gcattctagt tttaacagtt gaaggtaaag cggttcatgg tatggatcca tctatcggtg
    12721 tgaatgcggg tctttactta ctaaaattct tggcatcatt aaatcttgat aataatgcac
    12781 aagcgtttgt agcatttagt aatcgctact tatttgattc agattttggt gaaaagatgg
    12841 gaatgaaatt ccatacagat gtcatgggtg acgtgacaaa taacattggt gttattacat
    12901 atgataatga aaatgcgggt cttttcggta tcaacttgcg ctatccagaa ggatttgaat
    12961 ttgaaaaagc tatggatcgt tttgcaaatg agattcaaca atatggcttt gaagtgaaat
    13021 taggtaaagt ccaaccacca cattatgttg ataaaaatga tccttttgta caaaagttag
    13081 ttactgcata tagaaatcaa acaaatgata tgactgaacc ttatactata ggtggcggta
    13141 cttatgcgag aaacttagac aagggtgtag catttggcgc aatgtttagt gattctgaag
    13201 atttaatgca tcagaaaaat gaatatatca ctaaaaaaca gttatttaac gcaactagta
    13261 tttacttaga agcaatttat tcattatgcg tggaggaata atatatggaa aaaatttttt
    13321 taaatggtga gtttgtaagt ccaagtgaag caaaggtttc atacaacgac agaggatacg
    13381 tatttggcga tggtatttat gaatacattc gagtatataa tggtaagtta tttacagtaa
    13441 cagaacatta tgaaagattt ttacgtagtg ccaatgagat tggtttagat ttaaattatt
    13501 ctgtagaaga attaattgaa ctatctcgta aattagttga tatgaatcaa attgaaactg
    13561 gggcaattta tattcaagca acgcgaggtg tagctgaaag aaatcatagc ttcccaacac
    13621 ctgaagtaga accagcaatt gttgcttata caaagagtta tgatcgtcct tatgatcatt
    13681 tagaaaatgg tgtgaatggt gttaccgttg aagatatccg atggttacgt tgcgacatta
    13741 aaagcttgaa tttattagga aatgtattag caaaagaata tgctgtaaaa tacaatgccg
    13801 ttgaagcaat ccaacatcga ggcgaaactg tcactgaagg atcttcaagt aatgcttatg
    13861 caattaaaga cggtgtgatt tatacacatc cgattaacaa ctatattctt aatggtatta
    13921 cacgaattgt aattaaaaaa attgccgaag actataatat cccatttaaa gaagaaacgt
    13981 ttactgtaga tttcttgaga aacgcagatg aagttattgt ttcaagtact tcagctgagg
    14041 ttacacctgt tattaaatta gatggtgaac cagttaatga tggtaaagtt ggcccaatta
    14101 cacgtcaact acaagaagga tttgaaaagt atatagagtc acacagtatt taaaattctt
    14161 tcatcatatt tttagattaa attacttaaa tttatcaact ttcgtaagtg aattatgtta
    14221 taatttaaaa cgaagcttaa aagtattgtt tagaaaacac atattagtaa aaagtgccat
    14281 tttgtttctc attttcagtt gaaatataat gataaaaatt ataaaatttt ctatgagtta
    14341 caaatatgat gtgcatcata ttttactaag tacgtaaatg ttacagttca taaatttatg
    14401 ctaaccatga aaatgggata gcaattattt tgaaaactag agtttatcgc ctacaaaagt
    14461 tacaaaaaca tgtagtttta gacacggtaa cattttatgt caagtaagtg actatagaat
    14521 gtatggtgtc aatgacagac attacgattt aaatttagtc gtgtaatgta ggcattagtt
    14581 gaggatttac aataattatc tggacataat atttgtacca gtatggtgtt ataaattaga
    14641 atatatggct cttgaaaaac ctatttctag agccattttc taattgaaag tgaggtggac
    14701 gagttggagc agttttatca attagggtgg acacttgatt ctgcaggtgg tgcatctggc
    14761 gaagcatata tggctgaaca agatggacaa aagttgtttt taaaacgaaa ttcaaatcca
    14821 tttattgcgg cattatcagc agaaggtatt gtgcccaaat tagtatggac gaagcgcata
    14881 gaaacaggcg aggttgttac agcacaacat tggaaaaatg gccgtgaact atcttcaaac
    14941 gaaatgaagc aaacaagagt tgcacattta ttaaagaaga tacacaattc tagaccttta
    15001 ttaagtatgt taaagcgtat ggaaatggaa cctattactc ctgagattat gcttaataaa
    15061 attaatgcct ctttatcaag agaagtttta acacatcata ttgtgagaaa atcattaacc
    15121 tatttagaag agcatatacc gagtttagat tcacgttttt tcactgttgt acatggtgat
    15181 gtgaatcata ataattggtt attatctgat cgagatgaac tatttttagt cgattgggaa
    15241 ggtgcaatga tcgcagaccc ggcaattgat atcggaatgc tgctttacaa ctatgttcca
    15301 caacaacaat ggtctgaatg gcttgaaaca tatggtgttc aagagagctt gaatttaaat
    15361 aaacgtatga aatggtatac ggtaattcaa tctataggtc ttgtgcaatg gtatgaagag
    15421 caaaaaagat ataaggatat gaacacttgg cttaaattcc taaatcaagt tatgaatagt
    15481 aacatgttta tttaagagga gaataaaata tgagagttcg atacaaacca tgggctgaag
    15541 attatttaaa agatcatcct gaattggttg acatggacgg tcaacatgca gggaaaatga
    15601 ctgaatggtt tgataaaacg caaccgatac acattgaaat tggttcgggt atggggcagt
    15661 ttattacaac attagctgct caaaatcctc atattaacta tatttcaatg gaacgtgaga
    15721 aaagtatcgt ttataaagta ttggataaag ttaaagagat gagtttaaca aatttaaaga
    15781 ttatttgtaa tgatgccatt gaattaaatg aatattttaa agacggcgaa gtttcgcgta
    15841 tttatttgaa cttttcagat ccatggccaa aaaatcgtca cgcaaaacga cgtttaacgt
    15901 atcatacgtt tttagcgttg tatcaacaaa tcttaaatga tgaaggagat ttacatttta
    15961 aaactgataa cagaggacta tttgcctaca gtttagaaag tatgtctcaa tttggaatgt
    16021 attttacaaa aataaatcta aatttacatc aagaagacga tggcagtaat attctaacag
    16081 aatatgaaaa gaaattttcg aataaagggt cacgtattta tcgtatggaa gcgaaattcc
    16141 attcacaaaa ataaagcact tatgagggta tgtacacata caatcataag tgttttttcg
    16201 tactataatg gttctatagt aaaagggttc tataataaat cggactgata gaaaaagtat
    16261 tttacttttc atctgtccga ttttttgatt ttgaatataa aaaagcgcaa ttgcctctgt
    16321 aatttaaagt gaccaaacct aaactaaagg agacaattgc gcctatgaac aatgttatac
    16381 taaatacctt tgatttaaaa gaataaagct tgcattttac ttggaaattt taattattaa
    16441 tttaaaatta cagtccgatg gttatgatgc atttacatat aatatgattg cattaatctt
    16501 tataagtagt tagaagtgta ttagtgtggg gcgtttcgtt tttgttcgca tttatgaata
    16561 gtgatatgat gtaataaaca catgctaatt gtaataacga atacagaaag aataggtgat
    16621 gttgtgaaaa tcggggatat atctattcat tatctaaatg gcggcaatac aaaaatggat
    16681 ggcggtgcaa tgtttggtgt tgttccgaag ccgttgtggt caaagcaata caatgcaaat
    16741 gaacgaaatc aaatcaattt accgacacat ccaattttga ttcaaacggc tcaatataat
    16801 ttgattatag atgcgggtat tggtaatggt aaattatctg aaaagcaatt acgtaatttt
    16861 ggagtagatg aagaaagtca tattattact gatttggcaa attataattt aacgccaaag
    16921 gatattgatt atgtgctaat gacacatatg cattttgatc atgctgccgg tttgactgat
    16981 caagcgggac atgcaatttt tgaaaatgcg attcatgttg tgcaacaaga tgagtggcat
    17041 gagtttattg cacctaatat aagaagtaaa tcaacttact gggataagaa taaaggcgat
    17101 tatagtaaca agttgatttt attcgaaaaa cattttgaac cggttccggg tatcaagatg
    17161 caacatagtg gaggtcatag ctttggccac acgattatta cgattgaaag tcaaggagat
    17221 aaagcagttc atatgggtga tatattccca actactgcac ataaaaatcc tctatgggta
    17281 acggcatatg atgattatcc tatgcaatcg attcgtgaaa aagaacgcat gataccatat
    17341 tttattcagc aacaatattg gttcttgttt tatcatgatg aaaactactt tgctgtaaaa
    17401 tacagcgatg atggtgaaaa catagatgca tatattttac gtgaaacatt agttgataat
    17461 aactaaaata aagatgtatt actaaacaaa ttttcaaaaa taaaaaattg agccacatcc
    17521 aatcttacta attagggtgt ggctcatttt taagttttac gatccaaatc aaatatggat
    17581 aaaattcgta ttaacgctct acaatgttaa tgacttcacc agtatatgca tctgcataaa
    17641 aatcataatg aatattttga ccatttttaa tagttgtaat tccaccttga taaactaaac
    17701 ggtatttatc agtttcagga tgaacgaatg gttcataaac gatatatgag cctttgacat
    17761 tcataaaata tgattttact tcattaagta ctttaacggg attatagcgt ttacgattaa
    17821 tatattggat gattgaaatg gtagaaataa ttacaatggc tactgcaatt attgttggaa
    17881 ttatatattt cagtttagtc atcatttatg ctcccttgat ttcaaattca tatcattagt
    17941 ttaccatatt gaagatgata taataactta taaggagtga gaacttttat gaacataaat
    18001 aaaaaagtaa cattacaacg aattcaaact ttaactgagc ttcatggggc accaggtttt
    18061 gaagaagaag taaaaaatta tatgactcag caaatggcgc cgtacgtaga tgaatttatt
    18121 gaaaatcgta tgggtggatt ttttggtgtg aaaaaatcta aaaatccaaa tgcaaaacgt
    18181 gtaatgattg cagcacatat ggatgaaatc ggatttatga ttacaaatat cactaaaaat
    18241 ggaatgattc aattcacaaa tttaggtggt gttgcaaatg atatttggca aggacaacgc
    18301 ttagtaatta aaaatagaaa tggcgataaa attatcggtg ttgtttctaa tatacctaaa
    18361 cattttcgta ctggtagtga aggcgcaccg gaaattaaag atttaacatt agatataggt
    18421 gctcaaaatg aagatgaggt gcgtgagcgc ggaatagata taggagatac aattgtacct
    18481 cacacgccat tcacacagtt atctgaacat cgatatagtg ctaaagcatg ggataatcgt
    18541 tatggttgtg tcttggcaat tgaaatacta gaattattaa aagatataga attagatgta
    18601 gacttgtatg ttggcgcaaa tgttcaagaa gaggttggat tacgaggtgc gaaagcatct
    18661 gcagagatga tagacccaga cgttgcattt gtagtcgatt gttcacctgc caatgacgtt
    18721 aaaggaagcc aaccattatc tggtgaactt ggtaaaggga cgttaattcg cataaaagac
    18781 ggtacaatga ttttaaagcc tgtatttaga gactatttat taaagttagt agaagcacat
    18841 gacattgaac atcaatacta tatgtcacca ggtggaacag atggtggaga aattcataaa
    18901 gctaatattg gtattccgac tgcagttatt ggtgtatgtg cacgatatat tcatagtaca
    18961 gactcagtat ttgatataag agactatttt gcagctagat ctttactttc agaagccatt
    19021 tgtaatttag ataataatca aatagaaaca ttacaatata aataatcggg taataacaac
    19081 tattatctct aaatagttat atatattcat taattaagga gacataaaaa tgaaacaact
    19141 tgaatcagaa caacaatttg aatctttaaa acaaggtgct acagtatttg aattcactgc
    19201 aggctggtgt ccagattgta gagtgataga accagattta ccggaattag aagcgagata
    19261 tcctatgttt gacttcgtat cagtagaccg tgataaattt atggatattt gtattgaaaa
    19321 tggtattatg ggtattccaa gttttctagt atataaaaat ggagaactgc ttggaagtta
    19381 tattggaaaa gaacgaaaat caattgaaca gatagatgca tttttagctc aatacgtgta
    19441 atttagacta gagaaaaacg gggtaatact cgtttttctc tgttactatg tgttgattta
    19501 ttgtaaacta ttataaggtg cgaaattagg agtgttacat atgaatacct ttcaaatgag
    19561 agataaatta aaggaacgtt taagccattt agacgttgat tttaaattta atcgtgaaga
    19621 agaaactttg cgtatttatc gaacagataa taacaaaggt atcacgatta aacttaacgc
    19681 tatagtcgca aaatatgaag ataaaaaaga aaaaattgta gatgaaattg tttattacgt
    19741 tgatgaagct attgcacaaa tggcagataa aacccttgag agtatatcat ctagtcaaat
    19801 tatgcctgtc attagagcga ctagctttga taaaaaaact aaacaaggtg ttccttttat
    19861 ctatgatgag catactgcag aaacagcagt ttattatgca gtcgatttag ggaaatcata
    19921 tcgtctaatt gacgaaagca tgttagagga tttgaagtta actgaacaac aaataagaga
    19981 aatgtctctg tttaatgtta gaaaattgtc aaattcatat acgactgatg aagtaaaagg
    20041 taatattttt tattttatta actcaaatga cgggtatgat gcaagtagga tactaaatac
    20101 tgcattttta aatgaaattg aggcacaatg tcaaggcgaa atgctcgtag cagtgccaca
    20161 ccaagatgtg ttaattattg cagatatacg caataaaaca ggatatgatg tgatggcaca
    20221 tttaacaatg gaatttttca ctaaaggtct agttccaatt acatcattat cctttggata
    20281 taaacagggt catcttgaac cgatatttat tttaggtaaa aataataaac aaaaaagaga
    20341 tccaaacgtg attcagcgtt tagaagcaaa tcgtcgtaaa tttaataaag ataaatagaa
    20401 ataattggat aaggagtttt gtcataatga atttatttta caatcctaaa tatgtaggag
    20461 atgtcgcatt tttacaaatt gaaccagttg aaggtgaatt aaactacaat aaaaaaggta
    20521 atgttgttga aattactaat gaaggtaatg ttgtaggtta taatattttt gaaatttcaa
    20581 aagatataac aattgaagaa aaaggtcata ttaaattaac tgatgaactt gtaaatgtat
    20641 tccaaaagcg tatttcagaa gctggttttg attataaatt aaatgctgat ctatcaccga
    20701 aatttgtagt tggctacgtt gaaactaaag acaaacatcc tgatgcagat aaattaagtg
    20761 tactaaatgt aaacgttgga aatgacacat tacaaattgt atgtggcgcg cctaacgttg
    20821 aagctggaca gaaagttgtt gttgctaaag taggtgcagt gatgcctagc ggtatggtaa
    20881 ttaaagatgc tgaattacgt ggtgttgcct caagcggtat gatttgttca atgaaagaat
    20941 tgaatttacc taatgcacct gaagaaaaag gtattatggt attaaatgac agctatgaaa
    21001 ttggacaggc attttttgaa taattaagga aggtagtgaa aatatgagct ggtttgataa
    21061 attattcggc gaagataatg attcaaatga tgacttgatt catagaaaga aaaaaagacg
    21121 tcaagaatca caaaatatag ataacgatca tgactcatta ctgcctcaaa ataatgatat
    21181 ttatagtcgt ccgaggggaa aattccgttt tcctatgagc gtagcttatg aaaatgaaaa
    21241 tgttgaacaa tctgcagata ctatttcaga tgaaaaagaa caataccatc gagactatcg
    21301 caaacaaagc cacgattctc gttcacaaaa acgacatcgc cgtagaagaa atcaaacaac
    21361 tgaagaacaa aattatagtg aacaacgtgg gaattctaaa atatcacagc aaagtataaa
    21421 atataaagat cattcacatt accatacgaa taagccaggt acatatgttt ctgcaattaa
    21481 tggtattgag aaggaaacgc acaagtcaaa aacacataat atgtattcta ataatacaaa
    21541 tcatcgtgct aaagattcaa ctccagatta tcacaaagaa agtttcaaga cttcagaggt
    21601 accgtcagct atttttggca caatgaaacc taaaaagtta gaaaatggtc gtatccctgt
    21661 aagtaaacct tcagaaaaag ttgagtcaga taaacaaaaa tatgataaat atgtagctaa
    21721 gacgcaaacg tctcaaaata aacaattaga acaagaaaaa caaaatgata gtgttgtcaa
    21781 acaaggaact gcatctaaat catctgatga aaatgtatca tcaacaacaa aatcaatgcc
    21841 taattattca aaagttgata atactatcaa aattgaaaat atttatgctt cacaaattgt
    21901 tgaagaaatt agacgtgaac gagaacgtaa agtgcttcaa aagcgtcgat ttaaaaaagc
    21961 gttgcaacaa aagcgtgaag aacataaaaa cgaagagcaa gatgcaatac aacgtgcaat
    22021 tgatgaaatg tatgctaaac aagcggaacg ctatgttggt gatagttcat taaatgatga
    22081 tagtgactta acagataata gtacagatgc tagtcagctt catacaaatg gcatagagaa
    22141 tgaaactgta tcaaatgatg aaaataaaca agcgtcaata caaaatgaag acactaatga
    22201 cactcatgta gatgaaagtc catacaatta tgaggaagtt agtttgaatc aagtatcgac
    22261 aacaaaacaa ttgtcagatg atgaagttac ggtttcgaat gtaacgtctc aacatcaatc
    22321 agcactacaa cataacgttg aagtaaatga taaagatgaa ctaaaaaatc aatccagatt
    22381 aattgctgat tcagaagaag atggagcaac gaataaagaa gaatattcag gaagtcaaat
    22441 cgatgatgca gaattttatg aattaaatga tacagaagca gatgaggata ctacttcaaa
    22501 tatcgaagat aataccaata gaaacgcgtc tgaaatgcat gtagacgctc ctaaaacgca
    22561 agagcacgca gtaactgaat ctcaagttaa taatatcgat aaaacggttg ataatgaaat
    22621 tgaattagca ccgcgtcata aaaaagatga ccaaacaaac ttaaatgtca actcattgaa
    22681 aacgaatgat gtgaatgatg gtcatgttgt ggaagattca agcatgaatg aaatagaaaa
    22741 gcataacgca gaaattacag aaaatgtgca aaacgaagca gctgaaagtg aacaaaatgt
    22801 cgaagagaaa actattgaaa acgtaaatcc aaagaaacag actgaaaagg tttcaacttt
    22861 aagtaaaaga ccatttaatg ttgtcatgac gccatctgat aaaaagcgta tgatggatcg
    22921 taaaaagcat tcaaaagtca atgtgcctga attaaagcct gtacaaagta aacaagctgt
    22981 gagtgaaagc aagcctgcga gtcaagccgc accatcatca agatttgatt cacaagagtc
    23041 aaacacaaat gcatataaaa caaataatat gacatcaaac aatgttgaga acaatcaact
    23101 tattggccat gcagaaacag aaaatgatta tcaaaatgca caacaatatt cagagcagaa
    23161 accttctgct gattcaactc aaacggaaat atttgaagaa agccaagatg ataatcaatt
    23221 ggaagatgag caagttgatc aatcaacttc gtcttcagtt tcagaagtaa gcgacataac
    23281 tgaagaaagc gaagaaacaa cacatccaaa caatactagt ggacaacaag ataatgatga
    23341 tcaacaaaaa gatttacagc cttcattttc aaatcaaaat gaagatacag ctaatgaaaa
    23401 tagacctcgg acgaaccaac aagatgttgc aacaaatcaa gctgtacaaa catctaagcc
    23461 gatgattcgt aaaggcccaa atattaaatt gccaagtgtt tcattactag aagaaccaca
    23521 agttattgag tcggacgagg actggattac agataaaaag aaagaactga atgacgcatt
    23581 attttacttt aatgtacctg cagaagtaca agatgtaact gaaggtccaa gtgttacaag
    23641 atttgaatta tcagttgaaa aaggtgttaa agtttcaaga attacggcat tacaagatga
    23701 cattaaaatg gcattggcag cgaaagatat tcgtatagaa gcgcctattc caggaactag
    23761 tcgtgttggt attgaagttc cgaaccaaaa tccaacgaca gtcaacttac gttctattat
    23821 tgaatctcca agttttaaaa atgctgaatc taaattaaca gttgcgatgg ggtatagaat
    23881 taataatgaa ccattactta tggatattgc taaaacgcca cacgcactaa ttgcaggtgc
    23941 aactggatca gggaaatcag tttgtatcaa tagtattttg atgtctttac tatataaaaa
    24001 tcaccctgag gaattaagat tattacttat cgatccaaaa atggttgaat tagctcctta
    24061 taatggtttg ccacatttag ttgcaccggt aattacagat gtcaaagcag ctacacagag
    24121 tttaaaatgg gccgtagaag aaatggaacg acgttataag ttatttgcac attaccatgt
    24181 acgcaatata acagcattta acaaaaaagc accatatgat gaaagaatgc caaaaattgt
    24241 cattgtaatt gatgagttgg ctgatttaat gatgatggct ccgcaagaag ttgagcagtc
    24301 tattgctaga attgctcaaa aagcaagagc atgtggtatt catatgttag tagctacgca
    24361 aagaccatct gtcaatgtaa ttacaggttt aattaaagcc aacataccaa caagaattgc
    24421 atttatggta tcatcaagtg tagattcgag aacgatatta gacagtggtg gagcagaacg
    24481 cttgttagga tatggcgata tgttatatct tggtagcggt atgaataaac cgattagagt
    24541 tcaaggtaca tttgtttctg atgacgaaat tgatgatgtt gttgatttta tcaaacaaca
    24601 aagagaaccg gactatctat ttgaagaaaa agaattgttg aaaaaaacac aaacacaatc
    24661 acaagatgaa ttatttgatg atgtttgtgc atttatggtt aatgaaggac atatttcaac
    24721 atcattaatc caaagacatt tccaaattgg ctataataga gcagcaagaa ttatcgatca
    24781 attagagcaa ctcggttatg tttcgagtgc taatggttca aaaccaaggg atgtttatgt
    24841 tacggaagca gatttaaata aagaataatt atgagtaagg agttttatat aatgacacac
    24901 tatcattttg tcggaattaa aggttctggc atgagttcat tagcacaaat catgcatgat
    24961 ttaggacatg aagttcaagg atcggatatt gagaactacg tatttacaga agttgctctt
    25021 agaaataagg ggataaaaat attaccattt gatgctaata acataaaaga agatatggta
    25081 gttatacaag gtaatgcatt cgcgagtagc catgaagaaa tagtacgtgc acatcaattg
    25141 aaattagatg ttgtaagtta taatgatttt ttaggacaga ttattgatca atatacttca
    25201 gtagctgtaa ctggtgcaca tggtaaaact tctacaacag gtttattatc acatgttatg
    25261 aatggtgata aaaagacttc atttttaatt ggtgatggca caggtatggg attgcctgaa
    25321 agtgattatt tcgcttttga ggcatgtgaa tatagacgtc actttttaag ttataaacct
    25381 gattacgcaa ttatgacaaa tattgatttc gatcatcctg attattttaa agatattaat
    25441 gatgtttttg atgcattcca agaaatggca cataatgtta aaaaaggtat tattgcttgg
    25501 ggtgatgatg aacatctacg taaaattgaa gcagatgttc caatttatta ctatggattt
    25561 aaagattcgg atgacattta tgctcaaaat attcaaatta cggataaagg tactgctttt
    25621 gatgtgtatg tggatggtga gttttatgat cacttcctgt ctccacaata tggtgaccat
    25681 acagttttaa atgcattagc tgtaattgcg attagttatt tagagaagct agatgttaca
    25741 aatattaaag aagcattaga aacgtttggt ggtgttaaac gtcgtttcaa tgaaactaca
    25801 attgcaaatc aagttattgt agatgattat gcacaccatc caagagaaat tagtgctaca
    25861 attgaaacag cacgaaagaa atatccacat aaagaagttg ttgcagtatt tcaaccacac
    25921 actttctcta gaacacaagc atttttaaat gaatttgcag aaagtttaag taaagcagat
    25981 cgtgtattct tatgtgaaat ttttggatca attagagaaa atactggcgc attaacgata
    26041 caagatttaa ttgataaaat tgaaggtgca tcgttaatta atgaagattc tattaatgta
    26101 ttagaacaat ttgataatgc tgttgtttta tttatgggtg caggtgatat tcaaaaatta
    26161 caaaatgcat atttagataa attaggcatg aaaaatgcgt tttaatatgt ttataataga
    26221 gtagcatggg tatttattat taatgacatt attacatgtt aattaggagg cgtttttaat
    26281 ggattggatt ttaccaattg ctggaattat cgctgcgatt gcattcttaa ttttatgtat
    26341 cggtatcgta gctgtattaa attctgttaa gaaaaactta gattatgttg caaaaacact
    26401 tgacggtgta gaaggtcaag ttcaaggtat tactcgtgaa acaacagatt tacttcataa
    26461 agtaaaccgt ttaactgagg atatccaagg taaagtagat cgtttaaact cagttgtaga
    26521 tgctgttaaa ggtatcggtg actcagtaca aacgttaaac agctctgtag atcgtgtaac
    26581 aaattcaatt acacataata tttctcaaaa tgaagataaa atctcacaag ttgttcaatg
    26641 gtcaaatgtt gcaatggaaa ttgcagacaa atggcaaaat agacactacc gtcgtggaag
    26701 tgcaaattac aaagctaata atgtagcaac tgatgcaaat catagctata cttctcgagt
    26761 agataaataa ttaataatta aacaaatgca cttaaatgag gttgttactt atttaagtgc
    26821 atttgttata ggaggataca caaatgacgc aacaacaaaa taataaaaga acattaaaaa
    26881 ataaacacac ttatcaaaat gaaccattac ccaaccgtaa agattttgtt gttagtttta
    26941 taactggcgc gcttgttggt tcagctttag gtttgtattt taaaaataaa gtttatcaaa
    27001 aagcagatga tttaaaagtc aaagaacaag aactgtcgca aaaatttgaa gaaagaaaaa
    27061 cgcaacttga agaaacggtt gcctttacaa aagaacgtgt tgaaggattt ttaaacaaat
    27121 ctaaaaatga gcaagcagca ttaaaagcac aacaagcagc aataaaagaa gaagtaagtg
    27181 caaataattt gagtgataca tcacaagagg cacaagagat tcaagaagct aaaaaagaag
    27241 cacaagcaga aacagataaa agtacagctg tatcaaatga agaatcaaaa gcagcggcat
    27301 taaaagcaca acaagcagca ataaaagaag aagcaagtgc aaataatttg agtgatacat
    27361 cacaagaggc acaagagatt caagaagcta aaaaagaagc acaagcagaa acagataaaa
    27421 gtgcagctgt atcaaatgaa gaatcaaaag cagcggcatt aaaagcacaa caagcagcaa
    27481 taaaagaaga agcaagtgca aataatttaa gtgatacatc acaagaggca caagagattc
    27541 aagaagctaa aagagaagca caagcagaaa cagataaaag tgtagctgta tcaaatgaag
    27601 aatcaaaagc agtggcatta aaggtatcga aagaagagtc acaagctgaa agattagcaa
    27661 acgctgcaaa acagaagcaa gctaaattaa caccaggttc aaaagagagt caattaactg
    27721 aagcgttatt tgcagaaaaa ccagttgcta aaaatgactt gaaagaaatt cctcaattag
    27781 ttactaaaaa gaatgatgta tcagagtcag agacagttaa tatagataat aaagacactg
    27841 ttaaacaaaa agaagctaaa tttgaaaatg gtgttattac acgtaaagct gatgaaaaaa
    27901 caactaataa tacagctgtt gacaagaaat caggtaaaca atctaaaaaa acaacacctt
    27961 caaataaacg aaatgcatca aaagcatcta caaataaaac ttcaggtcag aaaaagcaac
    28021 ataataagaa atcatcacaa ggtgcaaaga aacaaagtag ttcaagtaag tcaactcaaa
    28081 agaataatca aactagtaat aagaattcaa aaacaacaaa tgctaaatca tccaatgcat
    28141 caaaaacgcc aaatgctaaa gttgaaaaag ctaataataa aatagagaaa cgtacattca
    28201 atgactaaga ttactaaaat cgtttaataa tcaatcatag cagtaagcta tgtattatat
    28261 ttttaaaaat tgaatttcaa aataaaaggt aggcattcaa gtgcttacct tttttgatat
    28321 catcagttta ttttgaaagg caacgcgatc attccatgta tttatgttgt ttgtaacatc
    28381 gttagattac aatagtgata ttgcacattt taagctgaag atggaaaaat attcgataat
    28441 caaataaaca atgaatctta gaaggtacaa tgacgttcac taatttaaat atagctgaat
    28501 gtgttggtga gtg
//
LOCUS       DBNENW010000015        28138 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNENW010000015 DBNENW010000000
VERSION     DBNENW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.015"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..822
                     /locus_tag="AC1LW8_000535"
     CDS             <1..822
                     /locus_tag="AC1LW8_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772400.1"
                     /translation="NDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQ
                     YHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVISVIA
                     VSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMI
                     EVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENIFGV
                     PGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            822..1892
                     /gene="opp3C"
                     /locus_tag="AC1LW8_000536"
     CDS             822..1892
                     /gene="opp3C"
                     /locus_tag="AC1LW8_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIP4772401.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAISSSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAGQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1908..2990
                     /locus_tag="AC1LW8_000537"
     CDS             1908..2990
                     /locus_tag="AC1LW8_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772402.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            2980..3921
                     /locus_tag="AC1LW8_000538"
     CDS             2980..3921
                     /locus_tag="AC1LW8_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772403.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIHD
                     EANNHLRQLHEIRPQHFVFSTEEEAAQLRENKLVTQN"
     gene            3940..5595
                     /locus_tag="AC1LW8_000539"
     CDS             3940..5595
                     /locus_tag="AC1LW8_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772404.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKGGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5807..7522
                     /gene="opp4A"
                     /locus_tag="AC1LW8_000540"
     CDS             5807..7522
                     /gene="opp4A"
                     /locus_tag="AC1LW8_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772405.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLTIDKKIRPKSYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKA
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVTN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAADPKELNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     DKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7573..8559
                     /locus_tag="AC1LW8_000541"
     CDS             7573..8559
                     /locus_tag="AC1LW8_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772406.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLVTVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKMDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8562..9542
                     /locus_tag="AC1LW8_000542"
     CDS             8562..9542
                     /locus_tag="AC1LW8_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772407.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGDEHQVACFYVNKVGDLNG"
     gene            9535..10497
                     /gene="opp4B"
                     /locus_tag="AC1LW8_000543"
     CDS             9535..10497
                     /gene="opp4B"
                     /locus_tag="AC1LW8_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIP4772408.1"
                     /translation="MVKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSISHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            10509..11390
                     /gene="opp4C"
                     /locus_tag="AC1LW8_000544"
     CDS             10509..11390
                     /gene="opp4C"
                     /locus_tag="AC1LW8_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIP4772409.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIISIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(11432..12421)
                     /gene="trpS"
                     /locus_tag="AC1LW8_000545"
     CDS             complement(11432..12421)
                     /gene="trpS"
                     /locus_tag="AC1LW8_000545"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIP4772410.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            12716..13111
                     /gene="spxA"
                     /locus_tag="AC1LW8_000546"
     CDS             12716..13111
                     /gene="spxA"
                     /locus_tag="AC1LW8_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HIP4772411.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            13482..14201
                     /gene="mecA"
                     /locus_tag="AC1LW8_000547"
     CDS             13482..14201
                     /gene="mecA"
                     /locus_tag="AC1LW8_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIP4772412.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            14322..15308
                     /locus_tag="AC1LW8_000548"
     CDS             14322..15308
                     /locus_tag="AC1LW8_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HIP4772413.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGFKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLK
                     NGKSKLVNDNLNYFKLKKFIVGLESKTAIIEKLINNYLNICSDRGNDVQILF"
     gene            15356..17164
                     /gene="pepF"
                     /locus_tag="AC1LW8_000549"
     CDS             15356..17164
                     /gene="pepF"
                     /locus_tag="AC1LW8_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP4772414.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(17624..18430)
                     /gene="yjbH"
                     /locus_tag="AC1LW8_000550"
     CDS             complement(17624..18430)
                     /gene="yjbH"
                     /locus_tag="AC1LW8_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HIP4772415.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(18453..18818)
                     /locus_tag="AC1LW8_000551"
     CDS             complement(18453..18818)
                     /locus_tag="AC1LW8_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin YjbI"
                     /protein_id="HIP4772416.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(18922..19515)
                     /locus_tag="AC1LW8_000552"
     CDS             complement(18922..19515)
                     /locus_tag="AC1LW8_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIP4772417.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQINYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            19701..20048
                     /locus_tag="AC1LW8_000553"
     CDS             19701..20048
                     /locus_tag="AC1LW8_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772418.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            20065..20700
                     /locus_tag="AC1LW8_000554"
     CDS             20065..20700
                     /locus_tag="AC1LW8_000554"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIP4772419.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            20717..21526
                     /locus_tag="AC1LW8_000555"
     CDS             20717..21526
                     /locus_tag="AC1LW8_000555"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIP4772420.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            21523..22377
                     /locus_tag="AC1LW8_000556"
     CDS             21523..22377
                     /locus_tag="AC1LW8_000556"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP4772421.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQENGENINPHIVTRLDRNTTGIVIFAKYGYIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            22398..23780
                     /gene="mgtE"
                     /locus_tag="AC1LW8_000557"
     CDS             22398..23780
                     /gene="mgtE"
                     /locus_tag="AC1LW8_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIP4772422.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDGKLVGVLSLRDLIVAENDAYIEDIMNERVIS
                     VNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGV
                     SDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGM
                     SGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQ
                     PLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFG
                     LATSFMAYLI"
     gene            23790..25634
                     /locus_tag="AC1LW8_000558"
     CDS             23790..25634
                     /locus_tag="AC1LW8_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIP4772423.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNNDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIVSKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            25912..26682
                     /gene="fabI"
                     /locus_tag="AC1LW8_000559"
     CDS             25912..26682
                     /gene="fabI"
                     /locus_tag="AC1LW8_000559"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIP4772424.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(26877..27962)
                     /gene="cozEa"
                     /locus_tag="AC1LW8_000560"
     CDS             complement(26877..27962)
                     /gene="cozEa"
                     /locus_tag="AC1LW8_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid biosynthesis protein CozEa"
                     /protein_id="HIP4772425.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT        10059 a         3866 c         5017 g         9196 t
ORIGIN      
        1 aacgatgcta aattaaatgc tgaacaaaaa gaaattttaa atgaaaaata tggattaaat
       61 gatcctgtag ctacgcagta tttacattat ttaaaaaatg ttgttacagg cgattttggt
      121 aattcattcc agtatcataa tcaacctgtg tgggatttga ttaaaccgag actactacct
      181 tcttttgaaa tgggtcttac agcaatgttc atcggtgtga tactgggact tattttaggt
      241 gttgcagcag ctactaaaca aaattcttgg gttgactata caactacagt tatttcagtt
      301 attgcagtat ctgtaccatc ttttgtactt gctgtacttt tacaatatgt atttgcagtt
      361 aaattaagat ggttcccagt agctggatgg gaaggttttt cgaccgcggt attaccgtca
      421 cttgcattat ctgcagctgt tttagcaact gtcgccagat acataagagc agagatgata
      481 gaggtattaa gttcagacta tattttatta gcgagagcta aaggtaattc gacaatgcgt
      541 gtactttttg gacatgcact tagaaatgct ttaattccaa ttattacaat tatcgttccc
      601 atgttagcaa gtattttaac aggcacttta acaattgaaa atatttttgg ggttcctgga
      661 ttaggggatc aattcgtacg ttcaattaca acaaatgatt tctcagtaat catggcaatc
      721 acactattat ttagcacact gtttatcgtt tctattttta ttgtagatat tttgtacggt
      781 gtgatagatc cacgaattcg tgttcaagga ggtaaaaaat aatggctgaa aataaaaaca
      841 atttgtcgat taacgacgat cattctaatg cagctatgac gcatacctct gacgctatct
      901 catcatctga ttttattatt agagaattag atttgaatca ggaacctgaa atgcaacgag
      961 aaagcaaaaa cttttggcaa gatgcttggg ctcagttaaa acgaaataag ttagctgttg
     1021 tcggtatgat aggtttaatt atcattgtaa tatttgcttt tatcggtcca gttataaata
     1081 aacatgatta tgctggacaa aatgtagaac atagaaatct tccggcaaaa atacctgtat
     1141 tagacaaagt tccattttta ccttttgatg gtaaagatgc agatggcaag gatgcttata
     1201 aagcagcaaa tgctaaagaa aattattggt ttggtactga tcagttgggt cgagatttat
     1261 ggacaagaac atggaaaggt gctcaaattt cattgtttat cggtgttgtt gcagcgatgt
     1321 tagatatttt tattggcgtt gtatatggtg cgatttctgg attcttcggt ggacgtgtcg
     1381 atacgattat gcaacgtata cttgaagtca tagcatctat tccgaattta attgtcgtaa
     1441 ttttatttgt attaattttt gaaccatcca tttggacaat tatattggct atgtctatca
     1501 caggctggtt aggcatgagc agagttgtac gtggagaatt tttaaaatta aaaaatcaag
     1561 agtttgtcat ggcttcgaaa acattggggg cttcaaaatt caaattgata tttaagcata
     1621 ttttacctaa tacattaggt gctatcgtgg ttacatcaat gtttacagta cctagtgcta
     1681 ttttcttcga agcattttta agtttcattg gtataggtgt acccgcacct caaacatcgt
     1741 tagggtcatt agtaaatgat gggcgagcaa tgttattaat ttatccacat gaattattta
     1801 taccagcaat gattttaagt ttattaattc tattctttta cttatttagt gatggattac
     1861 gtgatgcatt tgatccgaaa atgcgtaaat aaaagggggc atagcatatg actgaaagaa
     1921 tattagaagt aaatgatttg catgtttcct ttgatattac agcaggggaa gtgcaggcag
     1981 tgagaggtgt agatttttat ttaaacaagg gggaaacatt ggcaattgtt ggtgaatcag
     2041 gttcaggtaa atctgtaaca acaaaagcaa ttacaaaatt attccaaggg gacacaggaa
     2101 gaattaaaaa gggagaaatt ttatttttag gggaagattt agcaaaaaaa cctgaaaatg
     2161 agttaattaa attacgtggc aaagatattt caatgatctt tcaagatcca atgacatctt
     2221 taaacccaac tatgcaaatt ggtaaacaag tcatggaacc attaattaag cacaaaaatt
     2281 atagtaaagc acaagctaaa aagcgcgcat tggaaatact aaatcttgta ggtttaccaa
     2341 atgcagaaaa aagatttaaa gcatatccac atcaattttc aggtggacaa aggcaaagaa
     2401 ttgttattgc aaccgcatta gcttgtgaac ctaaagtgct cattgctgat gaaccaacga
     2461 ctgcattaga cgtaacgatg caggcacaaa ttttagattt aatgaaagaa ctacaacaaa
     2521 aaatcgatac tgcaattatt tttataacgc atgatttagg ggttgttgcg aatattgctg
     2581 atagagtggc agttatgtat ggtggtcaaa tggttgaaac aggagatgtt aacgaaatat
     2641 tttatgatcc aaagcatcca tatacatggg gattattatc gtcaatgcct gatttatcaa
     2701 caacaaatga cacaccatta ctagcgattc ctggagcgcc acctgattta ttacacccac
     2761 ctaaaggtga tgcatttgcg agacgtagtc aatatgcatt agatattgat tttaaagtag
     2821 aaccaccgtg gtttaaagtt tcaccgacac attttgtgaa atcttggtta ttagacgcac
     2881 gtgcaccaaa agttgaacta cccgagctgg taaaacaacg tatgaaaccg atgcctaata
     2941 attatgaaaa accactcaag gtagaaaggg tgtcgttcaa tgaaaaatga tgaagtgcta
     3001 ttatccatta aaaatttaaa gcaatatttt aacgcaggaa agaaaaacga agtgagagcg
     3061 attgaaaata tttcgtttga tatatacaaa ggggaaacat taggtttagt aggagaatcg
     3121 gggtgtggta aatctacaac tggtaaatca attattaaac ttaatgatat tacaagtgga
     3181 gaaattttgt atgagggtat tgatatacaa aagattcgta aacgtaaaga tttgcttaaa
     3241 tttaataaaa agatacagat gatttttcaa gacccatatg cgtctttaaa tcctaggtta
     3301 aaagtaatgg atatagtagc tgaaggtatt gatatccatc atttagcaac tgataaacgt
     3361 gaccgtaaaa aacgtgtcta tgatttactt gaaactgttg gattaagtaa agaacatgcc
     3421 aatcgctatc ctcatgaatt ttcaggtgga caacgccaac gtattggaat tgcccgtgca
     3481 ttagccgttg aaccagaatt tattatcgcg gacgaaccaa tatcggcatt ggatgtttca
     3541 atccaagctc aagtagttaa tttattatta aaattacaac gtgaaagagg gattacgttc
     3601 ctatttatag ctcatgatct atcaatggtg aagtatattt cagatcgtat tgcagtgatg
     3661 cattttggga aaatagttga aattggaccg gcagaagaaa tttatcaaaa tccattacac
     3721 gattatacta agtctttatt atcagccatt ccacaacctg atcctgaatc agaacgcagt
     3781 cgcaaacgat ttagttatat tcatgatgaa gcaaataatc atttaagaca attacatgaa
     3841 ataagaccac agcattttgt ctttagtact gaagaagaag cggcacaact acgagaaaat
     3901 aaattggtga cacaaaatta aggggaaggg ggaaatgcaa tgacgagaaa atttagaaca
     3961 cttattttaa ttttgattgc tacaattgca ttaagtggtt gtgctaatga tgatggtatt
     4021 tattcagata aaggtcaagt attcagaaaa attttgtcat cagacttaac atcccttgat
     4081 acatcattaa taacggatga aatatcttct gaagtgactg cgcaaacatt cgaaggttta
     4141 tacacattag gaaaaggtga caaaccggtg ttaggtgttg cgaaagcttt tcctgaaaag
     4201 agtaaaggtg gtaaaacttt aaaggttaaa ttaagaagcg atgctaaatg gagcaatggt
     4261 gacaaagtga ctgcgcaaga ttttgtttat gcttggagaa aaacagttga ccctaaaaca
     4321 ggttctgaat ttgcatacat tatgggggac attaaaaatg cgagtgatat tagtactggt
     4381 aagaaacctg tagagcaatt aggtatcaaa gcattagatg atgaaacatt acaaattgat
     4441 ttagaaaagc cggttccata tattaatcaa ttattagcac ttaatacctt tgcacctcaa
     4501 aatgaaaaag ttgccaaaaa atatggtaaa aattacggta cggcagctga tagagcggta
     4561 tacaatggtc catttaaagt tgatgattgg aaacaagaag ataaaacgtt actatctaaa
     4621 aatcagtatt attgggataa aaagaatgta aaattagata aagtgaatta taaagttatt
     4681 aaagacttac aagccggtgc atcattgtat gatactgaat cagtagatga cgcagttatt
     4741 actgcagatc aagtaaataa atataaagat aacaaaggat taaactttgt gttaacgact
     4801 gggacatttt ttgtaaaaat gaatgaaaaa caatatcctg attttaaaaa caaaaattta
     4861 agactggcta tcgcacaagc aatagataaa aaaggatacg ttgattcagt gaaaaacaat
     4921 ggctcaattc cttccgatac actaacagcc aaaggaattg cgaaagcgcc taatggcaaa
     4981 gattatgcga gtaccatgaa ttcgccttta aaatataatc ctaaagaagc aagagcacac
     5041 tgggacaaag ctaaaaaaga gttaggtaaa aatgaagtga cattttcaat gaacacagaa
     5101 gatacaccag atgcaaaaat atctgctgaa tatatcaaat cgcaagttga gaaaaattta
     5161 ccaggagtta ctttgaaaat taagcaatta ccgtttaaac aaagagtatc actagaactg
     5221 agtaacaatt ttgaagcatc acttagtggt tggtctgcag attaccctga tcctatggct
     5281 tatttagaaa caatgaccac aggtagcgca caaaataata cagactgggg taataaagaa
     5341 tatgatcaat tacttaaagt agcaagaacc aaattggcac ttcaaccgaa cgaacgatat
     5401 gaaaacttga aaaaagcaga agaaatgttc ctaggagatg caccggtagc accaatttat
     5461 caaaaaggtg ttgcacattt aacaaatcct caagtaaaag gattaattta ccataaattt
     5521 ggtccaaata actcacttaa acatgtatat attgataaat cgatagataa agaaacaggt
     5581 aagaagaaaa aataatatgc tttgtaaatt aggctggaga catatctcca gtctttttgt
     5641 gttggataaa aactttggga ataaaaattt aaaataagtc gttttttaaa ttactgaaat
     5701 tgattaaatg cataaataac tgaatattct aaaaataaac ttgtaataat tttttctatg
     5761 agtaaactaa aaagaaaaaa ttagattgaa agtaggaggc atatgtatgg ggaagcttat
     5821 taaatatatt tcaatacttc ttattgtcgt tttagtgttg agcgcttgcg gaaaaagcag
     5881 taataaagat gaaggagtaa aagatgctac taaaacagaa gcgtcaaaac ataaaggtgg
     5941 tacgctaaat gtagcgttaa cagcaccgcc aagtggtgtt tattcttcgt tattaaatag
     6001 tacacatgcg gatgctgtag ttgaaggata ttttaatgaa aacttattaa caattgataa
     6061 aaaaatacgt cctaaatcat atattgcttc atggaaggac atcgagccgg ctaagaaaat
     6121 agaatttaaa attaaaaaag gtattaaatg gcatgatggt aatgaattga aaattgatga
     6181 ttggatttat tcaattgaag tcttagctaa caaggattac gaaggtgctt attatccaag
     6241 tgtagaaaat atccaaggtg caaaagatta tcatgaagga aaagctgatc atattagcgg
     6301 attgaagaaa atagatgact acacaatgca ggttacattt gataaaaagc aagaaaatta
     6361 cttaacagga tttattactg gacctttatt aagtaaaaaa tatttatcag atgtaccaat
     6421 taaagattta gcgaaatcag ataaaatccg aaaatatcct attggtattg gaccatataa
     6481 agttaagaaa atcgtcccag gtgaggctgt tcaacttgtt aaatttgatg attattggca
     6541 aggtaagcct gcattagaca aaatcaattt aaaagttatt gatcaagctc aaattattaa
     6601 ggcaatggaa aaaggcgata ttgatgttac gaatgatgct accggtgcaa tggcaaaaga
     6661 tgctaagtca tctaatgctg gtctcaaggt attatctgcg ccaagcttag actacggttt
     6721 aataggattc gtatctcatg attacgataa aaaagctaat aaaactggta aagtgagacc
     6781 aaaatatgaa gacaaagaat tacgtaaagc aatgctttat gcaattgata gagaaaaatg
     6841 gatcaaagca tttttcaatg gttacgctag tgaaataaat agttttgtac catcaatgca
     6901 ttggatagca gctgatccta aggaactaaa cgattataaa tatgatcctg aaaaagctaa
     6961 aaaaatctta gataagttag gttataaaga tcgagatggt gacggattta gagaagatcc
     7021 taaaggtaat aaatttgaga ttaactttaa acattattca ggttcaaatc ctacttttga
     7081 accaagaact gctgcgataa aagatttctg ggaaaaagtt ggcttgaaaa caaatgtgaa
     7141 gttagtagaa ttcggtaaat ataatgaaga cttagcaaat gcatcaaaag atatggaagt
     7201 gtacttcaga tcgtgggcag gtggtacaga tccagaccca tcagatttat accacactga
     7261 tagacctcaa aatgaaatga gaacagtttt accaaaatca gatcaatatt tagatgatgc
     7321 gttagacttc gataaagtag gcattgatga aaagaaacgt aaagatattt atgttaaatg
     7381 gcaaaaatat atgaatgatg agttacctgg attaccaatg ttccaaggta aatcgataac
     7441 tatagttaat gataaagtac gaaacttaga cattgaaatt ggaactgatc aaagtttata
     7501 taatttaact aaagaagctt agtaatgtaa cctcgacgat tttgtcgagg tttacttata
     7561 tggggggatg ttatgaataa tgtattgtta gaggttaaag atttagaaac atcattaaaa
     7621 ataaataatg aatggttagt aactgttgaa aatatttctt ttgaattatc taaaggagaa
     7681 gttttgggta tagtagggga atctggttgc ggtaagtcca tattaagtaa gtcaattatt
     7741 aaattattac cagaaaagat atctaaacta agtaatggag aagttatatt tgatggtaaa
     7801 cgaatcgata cgctcaatga gaagcaattg ctagatattc gaggaaatga tattgctatg
     7861 atttttcaag aacctatgac tgctttaaat cctgtattta ccataaaaaa tcaacttgtg
     7921 gaatctataa aatcacataa aaaaatttct aaaaaagaag caaataactt agcaaaagat
     7981 ttactaaaaa aagttggaat tgctagacaa gatgaaatat taaatagcta tcctcatcaa
     8041 ttatctggtg gtatgagaca aagagtaatg attgcaatgg ccatttcatg ttctcctaaa
     8101 ttattaattg ctgatgaacc tacaacagca ttggatgtca cgattcaagc gcaaatatta
     8161 gacttattaa aagaattgca aaaggaaacg caaatggcaa ttatgatgat tacacatgat
     8221 ttgagtgtag ttgctgagtt ttgcgataaa gtcttagtta tgtatgcagg tcaaattgta
     8281 gaatttggag gcataaaaga aatactacac aatccgaaac atccttatac ccaaaaatta
     8341 ttatcaacaa ttccaaaact taaagaagag cagaaacgac ttgaaacgat agaaggaatt
     8401 gtgccatcaa tccaagcatt tcacgttaat aagtgcagat ttgcaaatag atgtaacaaa
     8461 aaaatggata tttgtaataa tcaatctcct aaaatgcatg tttgtgaaga cgtcattgta
     8521 cgttgtcatt tgtacaaaaa tgaatataag gagatataag aatggaaaat attttagaag
     8581 tcaaccaaat aaaaaaatac tacaaaatta aaactggatt attacaaaaa actcagtacg
     8641 ttaaagctgt tgatgacgta tcgttttcaa taaaaaaagg acaaactttt ggattagtag
     8701 gagaatcggg ttgtggtaag tcaacgttag gtaaagtgat tatcaggctt gaagatgcaa
     8761 cttcaggctc aataattgtt aatggtgaag atataacaag attacaaggt aaaaaactca
     8821 gaaaatcacg acaacaatat cagatgatat ttcaagatcc gtatgcatca ttgaatccga
     8881 tgcaaatggt tggagatatc atttcagaac ctattttaaa ttataaaaaa ttgccaaaag
     8941 aagaaataaa aaaagaagta ctatatttat taaaatgtgt tggcctaagt gaagatgcat
     9001 attataaata tgcacatgaa ttttcaggtg gacagagaca aagagtggga attgcaagag
     9061 cattggcttt gcgtccgagt ttaattgttg ctgatgagcc tgtaagtgca ttagatgtat
     9121 ctgttcaatc tcaagtactg aatttattaa aagatttaca agaacaattt aacttaagct
     9181 atttatttat cgcacatgat ttaagtgtag taaaacatat aagtgatgtc attggagtta
     9241 tgtatttagg tcatatagtt gaaatcgcat ctgataaaga aatttatgaa aatcccaaac
     9301 atccatatac aaaagcgttg atttcatcaa taccacaaat tgataaacat aataacaata
     9361 gaattatatt aaaaggagaa ttaccttcgc caagtaatcc gccgcaaggt tgtccttttc
     9421 atacaagatg tccgattgca aaagataaat gtaaagaaaa tataccacaa ttaaaggaca
     9481 ttggtgatga acatcaagtt gcttgttttt atgtaaataa agtaggtgat ttaaatggtt
     9541 aaattaatat taaagagatt aggtttaatg attccgttac taattttaat ttctattgtt
     9601 gtattttcat tagctatcat tcaaccagga gatccatttt cagatttaca aaacggaaaa
     9661 ataaaacaag aagcgataaa tgcacaaagg gaaaagttag gcctcaacga ctctatatca
     9721 catcaataca ttagatgggt caatcatgtt atacatggtg atttagggga gtcaatcaaa
     9781 tataaaaggc cggtaattga tgttattgag gaaagaattc caaatacaat attactcggt
     9841 gctatgtcat taattattac ttatattatc tcatttgctt taggaataac gtcaggtaga
     9901 tattcttaca gtttgactga ttatactgtg caaatattta attatttgat gttagctatt
     9961 ccatctttta ttgcgggagt atttgcaatt tttatttttt cttttgaatt acaatggttt
    10021 ccgtttcaag gttctgttga tattaacctt aaagaaggta cttttgaata ttatatgagt
    10081 aaaatttatc atacattttt gcctgcatta actttaggat tattatctac tgctggttat
    10141 attcaatatt tacgtaatga tattattgaa aattctaaaa aagattatgt attgacggca
    10201 aggtcaaaag gattatctat gaataaaatt tataataaac atatattgag aaattcttta
    10261 atacctatta ttacattttt aggtgctgat attgtaagta ttttaggtgg agctgtgatt
    10321 actgagacta tcttttcata taacggtatc ggtaaattat ttttagaatc ggtaataggt
    10381 caagactatc cattaatgat ggcattaacg ttgtttttct catttttagg tttactgggt
    10441 aatttgattt ctgatattac ttatggattt atagatccaa gaattagaag taactagggg
    10501 ggattggaat gcaaaataag tcaaaatcgc cttttaaaat tgcattgtct agatttattc
    10561 ataataaaat tgcaatgtta tcgattattt ttttattaat cataactatt atatcaatta
    10621 tagcgccatt aatagctcct tttccagtga accaacaaga tttattaaat ataaaaggtg
    10681 aaatgacagc acaaaacatt cttggtacag actctggtgg tagggataac tttagtcgtt
    10741 tgttatatgc aggtcgtatt tcattatcca ttggaattac atctacaata ggaatgcttt
    10801 tgattggaat tacagttgga gtgatttctg gttattttgg aggtattgtt gatacattat
    10861 taatgagaat aaccgaattt gttatgttat ttccattttt aatatttgca attgtattaa
    10921 atgctgcact tggagataaa attaaaaatc cttatggatc tgccataatt cttgttctag
    10981 ttattatcgt attaagttgg ggaggtattg caagacttgt tcgtggtaaa gtacttcaag
    11041 aaaaagaaaa tgaatacttt ttggcagcaa aatcaattgg tacacccaca tataaaatta
    11101 ttttgaaaca tcttttgccg aatatattaa gtgtagttat cgtacaagca acattgttat
    11161 ttgccggtat gattgtagtg gaatcaggat tgagcttttt aggattcgga attagtaaag
    11221 caataccatc ttggggtaat atgttgagtg atgctcaaga aggggatgtt ataagtggta
    11281 aaccgtggat atggatgcca cctgctataa tgattacatt aactatatta agtataaact
    11341 ttgtagggga agggcttaaa gatgctttta atcctagagg tagacgttaa ataataaaag
    11401 aggcactagt taattctagt acctctttat tttatctctt acgtcctaaa cccatcgctt
    11461 tttccatttt tttgacagtt ttaaatgaaa ctttgtgtgc tttatctcta ccttgatcta
    11521 aaatatcatc aagtttatct gagttataga aactttcgta tttttcttgg aattctacta
    11581 aaaatgcttt aactatttca gcaaggtcac ctttaaattt accataacct tcgccctcat
    11641 attttgcctc aatatcttta attggcatgt ctgttaatcc agcgtatatt gaaattaaat
    11701 ttgttatacc tggcttgttg tcacgatcaa atttaataat accatctgaa tcagttactg
    11761 cgcttttaat ttttttagct gcaacattcg gctcgtctaa taatgaaatg aagtttttag
    11821 cattatcatc actcttactc atttttcttg ttgggtcttg taaactcatg acacgtccac
    11881 caactttagg catacgaatt tcaggtttca caagcacatc attatagcga ctattaaatc
    11941 tatctacaag gttacgagtc aattcgatat gctgcttttg gtcatctcca actggaacga
    12001 tattagtatt gtaaagaaca atatcagctg ccattaaagg tggatatgtt aatagaccag
    12061 caggtatacc ttcaactgct ttctgagctt tatctttgta ttgcgtcata cgctctaatt
    12121 ctccaacaga agcaatcgta gttaacatcc atcctgcttg tacgtgtgca gggacttcag
    12181 attgtatgaa caatgttgct ttgtctggat ctataccaga agctaaataa atcgctgcta
    12241 attgtctggt ctgtttacgt aattttaaac gatcttgtgg cattgtaatt gcatgttgat
    12301 ctacgataca gaaataacaa tcatagtcat tttgcacatc aacaaattgt tttagtgcgc
    12361 caatataatt tccaatagta ggaattccac taggttggat gcctgaaaat aatgtctcca
    12421 tttaaatgcc tactttctta gtaatatttt aataattatc attataacag tatttcttaa
    12481 aaatgtaaga tgtgttagaa tatacgtata acttactact aacaaaggga ttaataatct
    12541 tttttataag tgtagaaact tatctgaaaa attaggtttt tctcatttaa ttttaatgtt
    12601 agatagttat aataaatagt aacgattaag aattttagaa aaaaatgttt tttcttgatc
    12661 tataactaaa ttttaagcaa tgaattttat tcattcaaat aggagagtga gatgtatggt
    12721 aacattattt acttcaccaa gttgcacatc ttgccgtaaa gcgaaagcat ggttacaaga
    12781 acatgacatt ccgtatacgg agcgtaatat tttttctgaa catttaacaa ttgatgaaat
    12841 taagcaaata ttaaaaatga ctgaagacgg tactgatgaa atcatttcta cacgttctaa
    12901 aacataccaa aaattaaatg ttgatattga ttcactacca ttacaagact tatattcaat
    12961 cattcaagat aatcctggct tattacgtcg tccaattatt ttagataata aacgactaca
    13021 agttggttat aatgaggacg agattcgacg tttcttacct agaaaagttc gtacgttcca
    13081 attacaagaa gcacaacgta tggttgacta ataaccattt aactatgtgt catactttac
    13141 taaaagatat gtcaagcaac actatttcta tagtgttgct tttttggttt taattttaca
    13201 ataattgctt agtatctttt aaatcaaatg tgcaattgtt aatatagctc tatattagag
    13261 tgttttggat gaaacatttg ctatgataag gtaaaattaa attaagaaag tgctttaatg
    13321 ctttaactgt ataaatcatt taaatagttt tgctacaacg aatatgttat tataagttac
    13381 caaaatattt tagagaaaga aatcaaacaa ataaattgta ttttgtttga tgctttaata
    13441 caatgtggat actattcatc gactgtaagg agtgagatga tatgagaata gaacgagtag
    13501 atgatacaac tgtaaaattg tttataacat atagcgatat cgaggcccgt ggatttagtc
    13561 gtgaagattt atggacaaat cgcaaacgtg gcgaagaatt cttttggtca atgatggatg
    13621 aaattaacga agaagaagat tttgttgtag aaggtccatt atggattcaa gtacatgcct
    13681 ttgaaaaagg tgtcgaagtc acaatttcta aatctaaaaa tgaagatatg atgaatatgt
    13741 ctgatgatga tgcaactgat caatttgatg aacaagttca agaattgtta gctcaaacat
    13801 tagaaggtga agatcaatta gaagaattat tcgagcaacg aacaaaagaa aaagaagctc
    13861 aaggttctaa acgtcaaaag tcttcagcac gtaaaaatac aagaacaatt attgtgaaat
    13921 ttaacgattt agaagatgtt attaattatg catatcatag caatccaata actacagagt
    13981 ttgaagattt gttatatatg gttgatggta cttattatta tgctgtacat tttgatagtc
    14041 atgttgatca agaagtcatt aatgatagtt acagtcaatt gcttgaattt gcttatccaa
    14101 cagacagaac agaagtttat ttaaatgact atgctaaaat aattatgagt cataacgtaa
    14161 cagctcaagt tcgacgttat tttccagaga caactgaata acgatattaa ttaagcaacg
    14221 cactatgaaa ggagactctt tggagtctct ttttttattg atttcaaaag ctgaaaaagt
    14281 aatagtagtt aaacttacgt gtaatatttt gaggtgaaga tatgttagta gctttaaatg
    14341 aagaaaagga acgtgtgtta gcaactactg cattgagaaa gacacaatat ttttgtccgg
    14401 tgtgtggcaa gcaagttatt ttaaagcgtg ggctcaaagt aattagtcat tttgcacata
    14461 aacatttagc ggaacaaaaa tgttttaata atgaaacgat taaacattat aaaagtaaat
    14521 tgattttagc acagatgata cagcaacaag gatttaaagt agagatagag ccatttttaa
    14581 aagaaataaa acaaattccg gatattttga ttaataataa atatgttatt gagctacagt
    14641 attcgccaat tccttataaa cagattcttc aacgaacgga aggtttaaag aaaatgggat
    14701 ataaagtaag ttggttatta aatgatgttg attattgtca taataaagtg aagttcaatc
    14761 attttcaaag tatgtttatt aatccattca ctcgaaaact tcatacgttc aatttagaga
    14821 aaaaacaaat aatgatgttt caacaaatac aatatttagg cgggcacaaa tatgtcgctg
    14881 aaaaaagaaa tgccaaaatt agtgagttgt ttaatgaggc gccttgtgat tatcatgctg
    14941 tttataaatt atcaaagttc gcaattaatc aatatatcaa atattgtcgc tggcaaaatt
    15001 ctgttttaga acccacttta agtgcaatgt atcaattaca gttaactgat caagaagtag
    15061 tgcacaatta tggttatatt tttccagagc aaatttatat taaaaatcat cctattgagt
    15121 ggcaattaca agttgattta tggttaaaga atggaaaaag caaattagta aatgacaatc
    15181 ttaattattt taaactgaaa aagtttattg ttggtctaga aagtaaaaca gcaattatag
    15241 aaaaacttat taacaattat ttaaatattt gttcagatag aggtaatgac gtgcaaattt
    15301 tgttctaaaa tgagaaaatt atatcaatta tattttatta ggaggtttac tacatatgag
    15361 tcaacaatta tctagagaag aacaggaacg taaatatcct gaatatacat gggacttaac
    15421 aacaattttc aaagatgatg aagcttttga ggctgcattt aaagaagttg aaaatgagtt
    15481 aggcaaagaa gaacaattta aaggacacat tggtgatagt gctgagacat tatacaatgc
    15541 attagaatta gaagatacat taggtactaa attagaaaaa gtatatgtat acgcgcacct
    15601 aaaacaagac caagatacaa cgaacgacaa gtatactggt atggagtcaa gagcacatca
    15661 attaattatt aaatttagct cggcatggag tttcttagtg ccagagattt tacaaattga
    15721 tgaagataaa attcaatcat ttgtaaattc atatgataaa ttacaaaaat tcgcatttga
    15781 tttgaagttg attaatgaaa aacgtcctca tattttagat gctgaaactg aaaagttatt
    15841 aacagaagcg caggacgcgt tatcaacgcc atcaaatgta tacggtatgt ttagcaacgc
    15901 tgatttagta tttgaagatg cgattgataa agatggaaat gcacacccgt taacacaagg
    15961 tacatttatt aagtatttag aatcagatga tcgcaaacta agagaaagtg cttttagaaa
    16021 tgtatataaa gcatatggtg ctcataataa tacgcttggc gctacgctag caggtgaagt
    16081 gaagaaaaat gtatttaatg ctcgtacaca caattacaaa actgcaagag aaaaagcatt
    16141 gagtaataat catattccag aaaatgtata tgacaatcta gtaaaaactg tacataaata
    16201 tttaccattg ctacatagat atactgaatt gcgcaaagaa ttgctaggtt tagatgactt
    16261 gaaaatgtat gatttatata caccattaat taaagatatt aagtttgaaa tgccttatga
    16321 agaagctaaa gagtggatgt taaaagcttt agaaccaatg ggtgaagaat atttaaatgt
    16381 agttaaagaa ggcttaaaca atcgttgggt cgatgtctat gagaataaag gtaaacgttc
    16441 aggtggctat tcatcaggtg cacatttaac taatccattt attctactta actggtctaa
    16501 tactatttca gacttataca cattagttca tgaatttggg cattcagcac atagttactt
    16561 cagtagaaaa ttccaaccgt caaattctag tgactacact atttttgtcg ctgaagttgc
    16621 atcaacttgt aacgaagcac ttttaagtga ttatatggat aaacatcttg atgatgaaaa
    16681 acgcttatta ttattaaacc aagaattaga acgtttcaga gctacattat tccgacaaac
    16741 aatgttcgca gaatttgagc ataaaattca tgcaattgaa gaagcaggtg aacctttaac
    16801 accaactaga atgaatgaag aatatgccaa attaaataaa ttatacttcg gtgattctgt
    16861 agaaactgat gaagatatta gtaaggaatg gtcacgtatt ccacacttct atatgaatta
    16921 ttatgtatat caatacgcaa ctggttacag tgcagctcaa agcttaagtc atcaaatttt
    16981 aacagaaggt aagccagcag tagatagata tattaatgaa ttcttgaaaa aaggtagctc
    17041 aaattatcca attgagatat taaaaaatgc tggtgtagat atgacaacac ctgaaccaat
    17101 tgaacaagct tgtgaagttt ttgaacaaaa attgaacgct tttgaaaaat taatgaaagc
    17161 ttagtactat tgtattcgct tacattatga acagattgtg acaatttaaa ttaagtacta
    17221 ttaaaaggtt gaaatggtag agatagcatg ttatattatg aacatgaaat taatcacata
    17281 acaaacatac ccctttgttt gaagtgaaaa atttctccca tcccctttgt ttagcgtcgt
    17341 gtattcagac acgacgtttt tttatgttct tttttaataa gagggaagtg aagttgtata
    17401 aaaatgtatt aaatatgcac tttttataca ttaacgatta atataagtct attatgtacc
    17461 aaatttataa tttgtataat tttcgaactg gttaaattcg gaaaatatca ctaatatatt
    17521 tagatcgatg tgtaacatta cgttctaata atttaatgtt gcaaaattaa gagtcagtaa
    17581 aaagacctcg ttacatttat ggtgtacgag gtctttaatt tatttatttt gatttgattt
    17641 taggcatttt agatttccag aaatcgccat ctggatattt aagtttttca attttttgtt
    17701 gaatggctaa cttctttaac tctttgttta aaagtttttc tggccattca taaatagtaa
    17761 gtaattcttc catcgttaca agttgttgtt gctgtatata agtttctaat ttaggaggaa
    17821 gattcttttc gataggttta cccatcaatt cattaattat ataagtatag atgtgatatg
    17881 ggtataatcc ttcgactttt aaaccttctt catgaacatc ttcactaaag aaaacgagag
    17941 aaggggcttg ttcgatttcc atttctcttg caatatgcaa atcaattttc aagctttcgg
    18001 ttagtttact tttttgtaag tcgtctttaa atacttctaa atcaatacct gcattttgaa
    18061 tacagtcaca aatcattgat tctgtaataa tatctctttt aggtatgatt tcattttgca
    18121 ttaaatgtat aaatcgttcg gcacgtacac gaccttgtaa ctcagctgct ttataagcta
    18181 gggcgatgtt atcaaagttg gatgtacttt gagcttggca tttcgttaat acttttaacg
    18241 aaggatttaa tatatgtctg atacgtatat attgattata ttcaattctt aatttggata
    18301 agattgctga taatttgaag caatcggagc taaatggatc gaaaaatgaa taaatttcga
    18361 ttttacttac aggtgataga ttaatatctt cacgactctt attttccatt attcgtaatt
    18421 ctccagccat gtttattcac ctacaattaa ttttaggaat tcaccatatg attagcagtt
    18481 aatcttaagc gctcaaataa ataatctcca acaccttgtg gaaacgcagc gcgattaatt
    18541 gctgtctgca tattttctaa ccatgcatct ctttcaaatt cagtgattgt aaaatccata
    18601 tgtctttttc ttagcatagg atgtccgtgt tcttcggtat aaatgtttgg accgcccaaa
    18661 aactgtgtta aaaattgttt ttgtttacga cttgtttctg caaaatctcc tggaaacagg
    18721 tgattaagtc gttcatcttt ttctacaagg gtgtaaaaat aatcaatcat atcgtataac
    18781 gcttctttac caatgatgtc atatggtgtt gttgtcattt aatcacccat tttcaaaaat
    18841 ttactgttac gaacttaagt taatatataa ctaatataac atgattttaa acatttgaaa
    18901 gaaatatgca tatttgccaa tttaatttat attgtttgaa agtgtttctt ttttcttgaa
    18961 aaaacgttga actttattta aaggttgatg atgttcgagg tttagttcgt ttaataaaga
    19021 ttggaacttt tgtaaacctt gattatagtc tttaacttcg aactctaact cataatccgt
    19081 agtatcgaaa tactcacttt tatctaaaac gagtaaatca cctttatatt tagtttcttg
    19141 gcgatatgtc gttaatgcac caagtattga taaagttgta tcctttacac caaactgttc
    19201 aactataatt tgacgaatgt catctggaag attgtcgttt gaaataatca agttcatctc
    19261 tggtttaatg tcgacgatat agttgtattc taatagacca acctttgctg gtgtctttaa
    19321 agtcatttca tattgattgt ctttaactct tatgcgtagt gcagagcgat gttcctttaa
    19381 tttgaaatcg ggtgtatcaa tatagtaatt gatttgctta aaaagcacac tgtctttaaa
    19441 atatttctct tgcaatttat tatagattga tgcagttatc atttgtttaa attctatttc
    19501 atgatttgtt gccatgatat gtatacacct cgtatcaaat tcaatttatc ttaactatat
    19561 tatgaatgac aaagttgaat tttaaaagca atttccttta tctattatca atgttaattt
    19621 gaccattaaa aatagtgttc gtaagtgttt tgtattattg aattgtgtta aaatgttatg
    19681 gaataagagg aggattaagc atgcgtttat atattaatga aattaaaatt aaagatgaca
    19741 tactttattg ttatacagaa gattctatta aaggattatc tgaagtagga caaatgctcg
    19801 ttgatagtga taattatgcc tttgcgtata cattagatga tggtaaagcg tatgcttatc
    19861 tcattttcgt acaagaaaca tggacgatgt tgcatgaaaa catgactaaa aaaattatta
    19921 tcaatgatga actagaattg actgaattcc accaagaact tacttatatt ttagacaaca
    19981 taaaagggaa taataattat ggtaaggaat ttgttgcaac cgttgaagaa acattcgaca
    20041 ttgaataaag cggggtgaag cactatgaat caatgggatc agttcttaac accttataag
    20101 caagcggttg atgagttgaa agtgaaactt aaaggcatgc gcaaacaata tgaagttggt
    20161 gaacaagcgt cgccaataga atttgttact ggtcgtgtta aaccaatcgc tagtattata
    20221 gataaggcaa acaaacgaca aataccattt gataggttaa gagaagaaat gtacgatatc
    20281 gctggtttaa gaatgatgtg ccaatttgtt gaagatattg atgttgtcgt caatatttta
    20341 agacaaagaa aagattttaa agtaattgaa gaacgagatt atattcgtaa cactaaagaa
    20401 agtggttacc gctcgtatca tgtcattatt gaatatccaa ttgaaacatt acaaggccaa
    20461 aaatttatat tggctgagat tcagattcgt acattagcaa tgaatttctg ggcaacgatt
    20521 gaacatactt tacgatataa atatgatggt gcttatccgg atgaaattca acatcgtttg
    20581 gaaagagcgg cagaagcagc gtatttactt gatgaagaga tgtctgaaat taaagatgaa
    20641 attcaggaag ctcaaaaata ttacacgcaa aaacgttcta aaaaacatga aaatgattaa
    20701 cgaggtgtta taaatcatgc gttatacaat tttaactaaa ggtgactcca agtctaatgc
    20761 cttaaagcat aaaatgatga actatatgaa agattttcgc atgattgagg atagtgaaaa
    20821 tcctgaaatt gttatttcag ttggtggtga tggtacatta ctacaagcat tccatcagta
    20881 tagccacatg ttatcaaaag tggcatttgt tggagttcat acaggtcatt taggatttta
    20941 tgcggattgg ttacctcatg aagttgaaaa attaatcatc gaaattaata attcagagtt
    21001 tcaggtcatt gaatatccat tgcttgaaat tattatgaga tacaacgaca acggctatga
    21061 aacaaggtat ttagcattaa atgaagcaac gatgaaaact gaaaatggct caacacttgt
    21121 tgtggatgtt aacttaagag ggaaacactt tgagcgattt agaggcgatg gattatgtgt
    21181 atcaacacct tcgggttcaa cggcttataa caaagcgcta ggtggcgcac tgatacatcc
    21241 ttcacttgaa gcaatgcaaa ttacagaaat tgcctcaata aataatcgtg tgtttagaac
    21301 ggtaggatca ccacttgtat taccaaagca tcatacatgt ttaatatcac cagttaatca
    21361 tgataccatt agaatgacga tagatcatgt tagtatcaaa cataaaaatg ttaattcaat
    21421 acaataccgt gtagcaaatg aaaaagtgag gtttgcacgt tttagaccat tcccattctg
    21481 gaaacgtgta cacgattctt tcatatcaag tgatgaagaa cgatgaaatt taagtatcat
    21541 atatcacaac aggaaactgt taaaactttt ttagcacgac atgatttttc taagaagaca
    21601 gtgagcgcca ttaaaaataa tggcgcttta attgttaatg atgaaccagt cacagtgcgt
    21661 aagcaattaa tgccaaatga tatattagaa attcatttac cacgagaaat accgagtgtt
    21721 aatttaatac cttatgctcg taagctagaa gtattgtatg aagatgcttt tatcatcata
    21781 gttactaaac caaacaatca aaattgtaca ccttcgagag aacatcctca tgaaagttta
    21841 atcgaacaag tactatatca ttgtcaggaa aatggtgaaa atattaaccc acatattgtt
    21901 acgcgtctag atcgtaatac aactggtatt gtgatattcg ctaaatatgg atatatccat
    21961 catttatttt ctaaagtaaa cttgaaaaaa atatatactt gccttgtata tggtaaaacc
    22021 catacatctg gtattattga agctaatatt agacggtcaa aggatagtat tataactaga
    22081 gaagttgcct cggatggtaa atacgctaaa acatcttatg aagtaataaa tcagaatgat
    22141 aaatacagtt tatgcaaagt tcatttgcat acgggacgta cacatcaaat tcgtgtacat
    22201 tttcaacata ttgggcatcc aattgtggga gattctttgt atgatggttt tcatgacaaa
    22261 attcatggtc aagtactgca atgtacgcaa atatattttg ttcatccaat caataagaac
    22321 aatatttata ttacaattga ttataagcaa ttacttaaat tattcaatca actctaattc
    22381 acacaggggg tgtaagtatg tcaatgaaca cagatgaaaa agagcgtgtt caagaggaat
    22441 tatatgatga gacattatta gatcaatact tagaaaatga tgatattgat caatttagag
    22501 atgaatttct agcattacac acatatgaac aaagtgagta ttttgaagat actaccgatg
    22561 aaaatagaca aaagattttt caatatttat cacctgaaga ggttgcaaat ttctttgatc
    22621 aattagatat tgatgacgat gaatatgagt tgctatttga taaaatgaat gcgacatacg
    22681 caagtcacat attagaagaa atgtcatacg ataatgcagt agatatttta aatgagttga
    22741 ctaaaccaaa agttgctagt cttttaacat tgatgaataa agatgacgcg aatgaaatca
    22801 aagcattact tcactatgat gaggatacgg ccggcggtat tatgacgacg gagtatttat
    22861 cacttaaagc gcatacgcct gttaaagaag cattattatt ggtcaaagcg caagcaccag
    22921 acgcagaaac aatatatgtt atatttgtcg ttgatgatgg taaattagta ggtgttttat
    22981 cgctaagaga tttaattgta gctgaaaatg atgcttatat tgaagatatt atgaatgaac
    23041 gtgtcattag tgtgaatgta gcagacgacc aagaagatgt tgctcaagtt atgagagact
    23101 atgatttcat ggctgtacct gttatagatt accaagaaca tttgcttggt atcatcacga
    23161 ttgatgatat tttagacgtt atggatgaag aggctagtga agactactct cgtttagccg
    23221 gggtatcaga tatcgattcg actaatgatt caatcattaa aacagcatta aaacgtttac
    23281 catggttgat tattttaaca tttttaggaa tgattactgc gacaatttta gggagattcg
    23341 aaaaaacatt agaaaatgta gcgctactcg cagcgtttat tcctattatt agtggtatgt
    23401 caggaaattc aggtacacaa tctttagccg tttcagttcg taacattacg acaggggaaa
    23461 ttaatgagca aagtaaattt agaattgcat taagagaagc aggaagtggt gtattatcgg
    23521 gtgttgtatg ttcaacaata ttatttacaa ttattgttgc aatatatcat cagccacttt
    23581 tagcattaat cgttgcagga agtttaactt gtgcgatgac ggtggggacg tttgtaggtt
    23641 cgatgattcc attattgatg aataaattaa atatcgatcc agcagtggct agtggaccat
    23701 ttattacaac aattaatgat attattagta tgttgattta ttttggttta gctacatcat
    23761 ttatggctta cttaatttaa ggaggagtta tggagttttt atctttagtt attgttgttt
    23821 tagcagcgtt tttaactcca ataattgtca atcgattaaa tattaatttc ttgccagttg
    23881 ttgttgcaga aattttgatg gggattgtga ttggaaattc atttctaaat atagtagaaa
    23941 gggattcaat tctaaatatt ttatcaacat taggctttat ctttttaatg tttttaagtg
    24001 gtttagaaat tgattttaaa gcttttaaaa aagataaacg cgcacgtcaa ggacaaaata
    24061 atgatgaatc ctcaattcca gggcatctta atctagcgtt aactgtattt gcatttatta
    24121 tgattatttc gattctttta gcgtatgtat ttaaatggct tggattagtg gatgatgtgt
    24181 tattaatggt cattatcatt tcaactattt ccttaggcgt agttgttcca actttaaaag
    24241 aaatgaatat tatgagaaca actatagggc aatttatcct attagtagca gtacttgcgg
    24301 acttagtaac tatgatttta ttaacggtct atggtgcaat caatggtcaa ggcggcagta
    24361 caatatggtt aataggtata ttagttgttt tcacagcaat ttcatatatt ttaggtgttc
    24421 aatttaaaag aatgtcattt ttacaaaaat tgatggatgg tacgacgcaa atcggtattc
    24481 gtgcggtatt tgcattaata atattattag tagccctagc agagggagtt ggcgcagaaa
    24541 atatattagg tgcattctta gcaggtgtcg ttgtttcatt attaaatcca gatgaagaaa
    24601 tggttgaaaa gttagactca tttggttatg ggttctttat tcctattttc tttataatgg
    24661 ttggtgtaga tttaaacata ccttcattaa ttaaagaacc gaaattacta attatcatac
    24721 cgattttaat cgttgcattt atcgtttcaa aattaattcc agtcatgttt attcgacgtt
    24781 ggtttgatat gaaaacaacg attgcatcag catttttatt aacatcaaca ttatcgctcg
    24841 tgatagctgc agccaaaatt tcagaaagat taaatgctat ttcagctgaa acgtcaggta
    24901 tattaatttt aagcgcagtc attacatgtg tattcgttcc gattattttc aaaaaactgt
    24961 ttccagttcc agatgagttt aaccgtaaaa ttgaagttag tttaattggt aaaaatcaat
    25021 taacgattcc tatagcgcaa aatttaacat ctcagttata tgacgtgaca ttatattatc
    25081 gcaaagactt gagtgatcgt cgtcaattgt cggatgatat cacgatgata gaaattgctg
    25141 attatgaaca agatgtttta gaacgactag gtctgtttga ccgagacata gttgtttgtg
    25201 ccacgaatga cgatgatatt aaccgaaaag ttgctaaatt agccaaagca catcaagttg
    25261 agcgtgtcat ttgcagactt gaaagcacaa cggacgatac agagttagtt gattcaggta
    25321 ttgaaatttt cagtagctac ttaagtaata aaatcttatt aaaaggttta attgaaacac
    25381 ctaacatgtt gaatttatta agtaatgttg aaacgtcact atatgaaatt caaatgttaa
    25441 attataaata tgaaaatatt caattacgta atttcccatt cggaggagac atcatcttcg
    25501 tgcgtattat ccgtaataat gagtcgattg ttccgcatgg agatacacaa ttgcgatatg
    25561 gagatcgctt aattgttacc ggtgctaaag aatacgttga tgaattgaag caagagttag
    25621 aattttattt ttaacaataa tgatattaaa atgcagacgt attttagtac gacgtaaaat
    25681 taatgatatt aaaatgctag tatgtatatg attttgataa ataaatgctt tttacgtaaa
    25741 tcaagtttga tacagaaagg actaaatcaa acatttatcg ttgtaatacg tttaaataac
    25801 tttattaaaa agtcataata gtgttaaaat gtattgacga ataaaaagtt agttaaaact
    25861 gggattagat attctatccg ttaaattaat tattataagg agttatctta catgttaaat
    25921 cttgaaaaca aaacatatgt cattatggga atcgctaata agcgtagtat tgcttttggt
    25981 gtcgctaaag ttttagatca attaggtgct aaattagtat ttacttaccg taaagaacgt
    26041 agccgtaaag agcttgaaaa attattagaa caattaaatc aaccagaagc gcacttatat
    26101 caaattgatg ttcaaagcga tgaagaggtt attaatggtt ttgagcaaat tggtaaagat
    26161 gttggcaata ttgatggtgt atatcattca atcgcatttg ctaatatgga agacttacgc
    26221 ggacgctttt ctgaaacttc acgtgaaggt ttcttgttag ctcaagacat tagttcttac
    26281 tcactaacta tcgttgctca tgaagctaaa aaattaatgc cagaaggtgg tagcattgtt
    26341 gcaacaacat atttaggtgg cgaattcgca gttcaaaact ataatgtgat gggtgttgct
    26401 aaagcgagct tagaagcaaa tgttaaatat ttagcattag acttaggtcc agataatatt
    26461 cgcgttaatg caatttcagc tggtccaatc cgtacattaa gtgcaaaagg tgtgggtggt
    26521 ttcaatacaa ttcttaaaga aatcgaagag cgtgcacctt taaaacgtaa tgttgatcaa
    26581 gtagaagtag gtaaaactgc ggcttactta ttaagtgatt tatcaagtgg cgttacaggt
    26641 gaaaatattc atgtagatag cggattccac gcaattaaat aatatcattc aacagctttg
    26701 ttcacgttat tatatatgtg agcaaagctt ttttgctttt ataataatcg gactgatgga
    26761 aaattatttg atatttcatt tgactgattt tttatgcaca aaaaaatcgc ctcaaaagct
    26821 gtagtttcaa cttttgatgc gatagtatct tatattctta aaagacatcc acagaattag
    26881 tccttaacat tactgtttgc tttatcaata atgcgttggc ggtatttgaa aatattacta
    26941 acaaccgttt taagtacagc atataatggc actgcaatca gaactaatgt aaagccacct
    27001 aaatctcctg ctgctaaaat aacaacgata attgttagag gatggatact taaagattta
    27061 cccattacat ttggcgtaat gatattacct tcaagttggt gtgcaattaa tgtaatgata
    27121 caaacccata taaatgtagt aggactatct ataataccga gtattgctgc aggtgcaaat
    27181 gataaccatg aacctaagaa aggaattaag tttgcgacac cagcaaatag tactaataaa
    27241 ggaatatatg gtaagtcaat aattgaataa ccgatatata agaatatacc taaaataaca
    27301 ctgacagtta cttgaccttg aatgtaagat tttaatgtaa agtttaaatc agttaataaa
    27361 tctacgaaaa atactttacg ttcacctttg aaaaatttag caacagctgg gataaatttt
    27421 tcatggtctt ttaacatata aattaagaag aatggaacca taatcaataa gaagatggtt
    27481 gaaattaatg atgtaatgta ctgtaatgaa ttagataaaa tattagtaac gccatcaccc
    27541 attgatttaa ccatatttgt aattctactt gttacatctt caggtaattt atccatttga
    27601 gctagtgcaa atttaataat ttgctctgct tctttttgta gggcaggtgt ctgactaatt
    27661 aaattgttga tatttgaaat gatgattggt gcaacaaacg caacaattaa gccgataata
    27721 gcaaacaaag ctaacatgat tgttgttata ctagcccatc ttggaaaacc gactttttca
    27781 agtaagtttt gaaaaggtag acagatataa aataaaaaac cactaattaa aaatggaaga
    27841 aatacagaac cgatgattgt agctattgga gtaaatactt cgtgcacttc cataaatagt
    27901 ttgatgagaa tgaacagcat aatcagagcg atgccagttc ggaaccaaac cttgtttaac
    27961 ataaacgatg ttcctcctat ttttctaaac ttagttatta tttagtatat cctaaatagc
    28021 agtgcttaaa aaggttaata ttttttaaat atacttaaat taaccattca ctttctattt
    28081 taagatattt actttcaata gtttgtgaga cttttcaatg actcgtaagt ctgcaatt
//
LOCUS       DBNENW010000016        27877 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNENW010000016 DBNENW010000000
VERSION     DBNENW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.016"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            205..1395
                     /locus_tag="AC1LW8_000561"
     CDS             205..1395
                     /locus_tag="AC1LW8_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HIP4772426.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1504..2748
                     /locus_tag="AC1LW8_000562"
     CDS             1504..2748
                     /locus_tag="AC1LW8_000562"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIP4772427.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3138..4067)
                     /locus_tag="AC1LW8_000563"
     CDS             complement(3138..4067)
                     /locus_tag="AC1LW8_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIP4772428.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4309..5688)
                     /gene="argH"
                     /locus_tag="AC1LW8_000564"
     CDS             complement(4309..5688)
                     /gene="argH"
                     /locus_tag="AC1LW8_000564"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIP4772429.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLINQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5678..6883)
                     /locus_tag="AC1LW8_000565"
     CDS             complement(5678..6883)
                     /locus_tag="AC1LW8_000565"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIP4772430.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDAADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNSM
                     LHGGYSNEQ"
     gene            7234..8565
                     /locus_tag="AC1LW8_000566"
     CDS             7234..8565
                     /locus_tag="AC1LW8_000566"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIP4772431.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8890..9465
                     /locus_tag="AC1LW8_000567"
     CDS             8890..9465
                     /locus_tag="AC1LW8_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIP4772432.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9470..9994
                     /gene="lepB"
                     /locus_tag="AC1LW8_000568"
     CDS             9470..9994
                     /gene="lepB"
                     /locus_tag="AC1LW8_000568"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP4772433.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            10010..10585
                     /gene="lepB"
                     /locus_tag="AC1LW8_000569"
     CDS             10010..10585
                     /gene="lepB"
                     /locus_tag="AC1LW8_000569"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP4772434.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10745..14221
                     /gene="addB"
                     /locus_tag="AC1LW8_000570"
     CDS             10745..14221
                     /gene="addB"
                     /locus_tag="AC1LW8_000570"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HIP4772435.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVFLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYIAMTRARQDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14222..17875
                     /gene="addA"
                     /locus_tag="AC1LW8_000571"
     CDS             14222..17875
                     /gene="addA"
                     /locus_tag="AC1LW8_000571"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIP4772436.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18041..18943
                     /locus_tag="AC1LW8_000572"
     CDS             18041..18943
                     /locus_tag="AC1LW8_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIP4772437.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            19272..19661
                     /locus_tag="AC1LW8_000573"
     CDS             19272..19661
                     /locus_tag="AC1LW8_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HIP4772438.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWI
                     TMALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(19833..21149)
                     /locus_tag="AC1LW8_000574"
     CDS             complement(19833..21149)
                     /locus_tag="AC1LW8_000574"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HIP4772439.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(21201..22025)
                     /locus_tag="AC1LW8_000575"
     CDS             complement(21201..22025)
                     /locus_tag="AC1LW8_000575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP4772440.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            22139..22447
                     /locus_tag="AC1LW8_000576"
     CDS             22139..22447
                     /locus_tag="AC1LW8_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIP4772441.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            22995..24809
                     /locus_tag="AC1LW8_000577"
     CDS             22995..24809
                     /locus_tag="AC1LW8_000577"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP4772442.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            25012..27621
                     /gene="clpB"
                     /locus_tag="AC1LW8_000578"
     CDS             25012..27621
                     /gene="clpB"
                     /locus_tag="AC1LW8_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIP4772443.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(27680..>27877)
                     /locus_tag="AC1LW8_000579"
     CDS             complement(27680..>27877)
                     /locus_tag="AC1LW8_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4772444.1"
                     /translation="LIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKK
                     ESPEILVFIKSFKKYIANEQL"
BASE COUNT         9703 a         4038 c         5044 g         9092 t
ORIGIN      
        1 gtaaaattat caattctttt gcttgtaatc atttaagtaa ttatgtcatt ataaatgtaa
       61 gggttttcaa aatagacaaa tttaatgaaa cttagtgatt atttcatctt ttttggtgga
      121 aaagtatctg ctttgaaaac taatgtaatt atttggataa tgtttcatgt ataatagtta
      181 taaatgatga aaaggggaga tatgatgact aaatctgaaa aaattattga gttaacaaat
      241 cattacggag cacataatta tttaccattg ccaattgtca tttcagaagc tgaaggggta
      301 tgggttaaag atcctgaagg caataaatat atggatatgt tatctgcata ttccgctgtt
      361 aaccaaggtc atagacatcc gaaaattatt caagcattaa aagatcaagc tgataaagtg
      421 actctagttt cacgtgcttt tcatagtgat aacttaggtg aatggtacga aaaaatttgt
      481 aaactggcag gtaaagataa agctttacca atgaatacag gtgctgaagc agtagaaaca
      541 gctttgaaag cagcacgacg ctgggcatac gatgttaaag gaattgagcc aaataaagca
      601 gaaatcattg catttaatgg taacttccat ggtcgaacaa tggcgccagt ttcattatct
      661 tcagaagcag aataccaacg tggttatggt ccgttattag atggatttag aaaagttgat
      721 tttggagatg tagatgcatt gaaagctgca attaatgaaa atactgcagc agttttagta
      781 gaaccaattc aaggtgaagc gggtataaat ataccgccag aaggatattt gaaagcaatt
      841 agagaattat gtgatgaaca taatgtctta tttattgctg acgaaatcca agcaggatta
      901 ggtcgttcgg gtaaattatt tgctacggat tgggataatg taaaacctga tgtctatatt
      961 ttaggtaaag cactaggtgg tggtgtcttc ccaatttctg ttgtattagc agataaagaa
     1021 gtattagatg tctttacacc tggctcacat ggttcaacat ttggtggtaa tccacttgct
     1081 tgtgctgcat caattgctgc attagatgtt atcgttgatg aggatttacc agggcgctct
     1141 ttagaattag gagattattt taaagaacaa ttaaagcaaa ttgatcatcc atcaattaaa
     1201 gaagtccgtg gacgtggttt gtttataggt gtggaactta atgaaagtgc tagaccatat
     1261 tgtgaagctt tgaaagaaga aggcttatta tgtaaagaaa cgcatgatac tgtcattcgt
     1321 tttgcaccac cattaattat tactaaagaa gaattggacc ttgcacttga aaaaataaga
     1381 catgtatttc aataatgtca aaaagttgta acaatcagtt tgtaagggct ttaaaaagga
     1441 atacgttatg ttacaatatg tttgaaagcg aaatcattag gtaaagaaga ggcgaaaagg
     1501 atcatgactg agaacaataa tttagtaact tctactcaag gaattattaa agaagcattg
     1561 cataaattgg gatttgacga aggaatgtac gatttaatta aagaaccttt aagaatgtta
     1621 caagtgcgta tccctgtacg aatggatgat ggcacagtta aaacattcac aggttaccgt
     1681 gcgcaacata atgatgctgt tggaccaaca aaagggggcg tgcgtttcca cccagatgtt
     1741 gatgaagaag aagtaaaagc attatcaatg tggatgactt tgaaatgtgg cattgtaaac
     1801 ttaccatacg gtggtggtaa gggtggtatc gtttgtgatc cacgtcaaat gagcattcat
     1861 gaagttgaac gtttatcacg cggatatgta agagcaattt cacaattcgt aggtccgaac
     1921 aaagatattc cagcaccaga tgtatttaca aactcacaaa ttatggcttg gatgatggat
     1981 gaatatagtg cattagataa atttaattca ccaggtttca tcacaggtaa accaattgta
     2041 ttgggtggtt ctcatggacg cgacagatca actgcactag gtgtagttat tgcaattgaa
     2101 caagctgcaa aacgtcgtaa tatgcaaatt gaaggtgcca aggttgttat tcaaggtttc
     2161 ggtaatgccg gaagtttctt agctaaattc ttatatgatt taggtgcaaa aattgtaggt
     2221 atctctgatg cttacggtgc attacacgat ccaaatggct tagatataga ttatttatta
     2281 gaccgtcgtg atagttttgg tacggtaaca aatttatttg aagaaacaat ctcaaataaa
     2341 gaattgtttg aattagattg tgacatttta gtaccagcgg ctatttcaaa ccaaattaca
     2401 gaagacaatg cacatgatat taaagctagt atcgttgttg aagctgctaa tggacctaca
     2461 acaccagaag caacacgtat tttaactgaa cgtggtatat tattagttcc agacgtatta
     2521 gcaagtgctg gtggtgtaac ggtttcttac ttcgaatggg tacaaaataa tcaaggttat
     2581 tattggtctg aagaagaagt taatgaaaag ctacgtgaaa aattagaagc ggcatttgat
     2641 acgatttacg aattgtctca aaaccgaaaa atagatatga gacttgcagc atatatcata
     2701 ggtattaaac gtacagcaga agcagctaga tatcgtggtt gggcataatt aattatcata
     2761 tgtgatttaa cgagcttggg acagaaaaca aagccctaag ctcgttaatt ttattttagc
     2821 agtagttgac tgtaaaacaa tgcccgtgta acacgctctt ttcaaaaata gtcggggccc
     2881 caacacagag aatttcgaaa agaaattcta caggcaatgc aagttgggga tgggccccaa
     2941 cacagagaaa ttggggagcc aatttctaca gacaatgcaa gttggggtgg gacgacgaaa
     3001 taaattttgc gaaaatatca tttctgtccc actcccaaaa agacagcagt aggataattt
     3061 caatttggaa atatcttact gctgtttttt atagtaatag ctatattttt gtatttatgt
     3121 tttctagttt aacattacta cttaatgact tctttatatt tatcagcgaa atttgtaaag
     3181 acaccatcaa cgccatattt atttaatcgt aacatatcag ctttttcatt cactgtataa
     3241 ggatgtacta taaatcctaa gtcttttaaa tgatgggtat tttgttcagt taaatctgta
     3301 taatcaggac ctaatccaat cgcataagag cgtatctctt ttaagcgttg gtcgttaaat
     3361 tgttgtagtt cacctttatc aactaatttt actaatggca catgcttatt ttgacgatga
     3421 attttcttta aactttcgtc agaaaatgat tgaatcatta catgtccatt ttttaattta
     3481 ttgttattta aaaggtgatg ctttttcaat gaagctaata attgttcttc cattcctggg
     3541 tatacatcag gtgactttgt ttcaatataa tagtttgcat tcgggccata acgttctaaa
     3601 atttcatcta aagtgggtac tttagcattt ttataacttg ctcttgcgta ttttggatat
     3661 tttttattaa accaacttcc tgcatctaac tgttttaatt catcaagggt ataatcctca
     3721 actttaccgt gtccatttgt tgtacggtta acagtttcat catgcatagc aactaaatgg
     3781 ccatctttgg tacgttgtaa atcaatttcg atataagatg cttttaactc attatgactc
     3841 ttatcatatg cttgaaacgt atgctcgggt gcatagccac ttgcgccacg atgtgcgata
     3901 gtagtgaatc gctcattcgt taaatttgta tgccattgaa tagcctgagg tttatttgca
     3961 atttgatttg tttgttcagc gccagcagta ggtactgata aaaatcccat agtaaaaaca
     4021 gcagaagcag ccataaattt agtgaagctt ttcgaagagt tagtcatagt ctttatcctt
     4081 tcatctcttt atttgtagtc taacaatttt aaaatactac aaaaacctgc ttgtgaaaac
     4141 gctttacaca tttcttttac aatttataac atttttgtaa tataaattta aaagaattat
     4201 caaacatttt aggaatctta aaaatgtgat gttcaacaag acgaagagag cattaaaata
     4261 cccacaatgt aattttaata gacattgtgg gtaggtagat taacgtattt attgtgatag
     4321 taattgttta gcaacatcaa gttgttgttt gaccgatgat tgacctgttg aaccgtaact
     4381 ttgacgtcgt tttaaacaat tttcaggctg caaataatcg taaatatcgg catcaatact
     4441 agaatgatgt tgttgatatg ttgctaaagg aacatctaat aaataatgac cttgttgtat
     4501 acattctaag acgatttttc ctacaatttc atgtgcagtt ctaaatggaa tatttttagt
     4561 tactaaataa tctgctagtt ccgttgcatt tgaaaaatct tctttaacag tttgattgag
     4621 tcgttcttta ttaattgtca tcgtttgaat cataccttcg aaaatacgta aagaaccttt
     4681 aattgtatgg acagcatcga ataaaccttc tttatcttcc tgcatatctt tgttatatgc
     4741 tagaggtaat ccttttaaag tcataagcat gctcattaaa tgaccagtcg ttcgaccaac
     4801 tttacctcta attaattctg ccatatcagg atttttcttt tgtggcataa tagatgagcc
     4861 agttgaaaat gcatctgata atgtaatgaa tttagcttcg tctgtggacc agaaaataat
     4921 ttcctctgca aagcgtgata agtgaaccat cgttaaagaa atattatgca atgtttcaat
     4981 aatatagtct ctgtcactaa cagcatctag gctattctca tagagactgc caaagttcaa
     5041 caatgctgtt gtctcgtgtc tatcgatagg gtatgtggta ccacttaagg ctgctgcacc
     5101 taaaggatta atatcgattc gttttaaact atcttcaaat cgttgttggt ctcgttgtaa
     5161 catccaaaaa taagtcataa tatgatgtgc aaatgaaatt ggctgtgcac gctgtaaatg
     5221 agtataacca ggcataattg tatcaacatt attggaagcg atgtctacaa ttacactttg
     5281 taacgactta attaatgcga tgatatcttg cacttgtttc ttagtgtaca agtgcatgtc
     5341 tgttgcaact tgatcgtttc tactgcgtcc agtatgcaac ttaccaccag catcaccaat
     5401 acgtttaatt aattcatgtt caatatttaa atgaatatct tctaatgatg cactaaattg
     5461 aatttgatct tgatgataat catgttgaat agattttagt ccttgtataa tttgttcgct
     5521 gtcttgttga ctaataatgc cttgattcgc aagcatagtt gcatgtgcaa tgctgccttc
     5581 gatatcttga tttatgagcg tttgatcaaa agtaatggat gcgttaaagt cgtcaaccca
     5641 ctcttcaggt tgtacttcaa atctaccgcc ccaagcttta ttgctcattg ctatagcctc
     5701 catgtagcat cgaatttact tgagtaggta aaccatagat atcgataaag ccaacagcag
     5761 cgtcttgatt aaatgcatct tcttttgtat aagttgctaa tttttcatca tataatgtgt
     5821 aaggtgattt tctaccattt acgatggcat tacctttgaa taatttaatt ctgacatcac
     5881 cacttacgta ttgctgagta ctatcaataa ataattttaa gctatcagtt aaaggtgaga
     5941 accaaagtcc attgtatagt tgttcagcaa attgcttctc gatgattggt ttaaagtgtg
     6001 cgacatcttt cgttaacgta atcgtttcta atgctttatg cgcttttaaa ataacttctg
     6061 cagcaggtgc ctcataaatt tctcttgatt tgatacctac aagtctattt tctacatggt
     6121 caattcttcc gataccatgc ttaccagcta atgcattcaa cgttaaaatt aaatcgtcta
     6181 attcatatgt tttgccatca atttgaactg ggatgccttt atcaaacgtt aaaatgattt
     6241 catcagcagc atctggtgtt tcttctaaag catttgttag atcgaacgca tcctctggtg
     6301 gcgcagcata aggatcttct aaaataccac attcattcgc tctgccccat agattttgat
     6361 cgatagaata aggtgaatca tggttgattg atacagggat attatgttta attgcataat
     6421 cgatttcttc ttcacgactc catgcccact cacgtacagg tgcgaatgct ttcaatgatg
     6481 ggtttaatgc tttaatggca acttcgaaac gtacttggtc attcccttta ccagtacaac
     6541 catgtgcaat acctactgaa tttgttttct cagcaatctc tactaatttt ttagcgatta
     6601 atggtcttga taaagctgaa actaatggat atgcattttc atacattaaa tttcctttga
     6661 ttgcataact tacatactca tcactaaatt cttttgttgc atcaataata tgacattcaa
     6721 ctgctcccat atctaaagct tttttataaa cgatgtctaa atctttacct tcaccaacat
     6781 ctaggcaaca agctacaacg tcgtatcctt tgtcgataag ccattgaacg gccacacttg
     6841 tatctagtcc tcctgaatat gctaaaacaa ttttctcttt cataaaattc acctcattgt
     6901 agttatctga atatttcgta tgaacacatg atagcaaact tttagtgaaa ataaaaccat
     6961 tattttacat atttattcat aaaatacgat agagatagtt agagttgtaa gtataaatgc
     7021 ttatttatca atgtttaaaa gtattttaaa aaacatgcaa attgtataaa aaatgtttga
     7081 gtgtttgacg ccagtttgaa atgtttgttg aaaaataggt gtgaaacgtc acataaatat
     7141 caattttatt gattaagtac aaacttgtga attgaatcgc aaacgtaaac ttagtaaaat
     7201 taagatatta aatgaaacag gaggctttta tagatgactc atattcaatt agattttagt
     7261 aaaacgttag aatttttcgg tgaacacgaa ttaaaacaac aacaagaaat tgttaaatca
     7321 attcacaaaa caattcatga aggtactggt gcaggtagtg acttcttagg ctgggttgat
     7381 ttaccagttg attacgacaa agaagaattt tcaagaattg ttgaagcatc aaaacgcatt
     7441 aaagaaaatt ctgatgtttt agtagtcatc ggtattggtg gttcttactt aggtgcacgt
     7501 gcagcaatcg aaatgttaac gtcatcattt agaaacagca atgaataccc tgaaattgta
     7561 tttgttggta atcacttatc atcaacatat acgaaagagt tagttgatta tttagcagac
     7621 aaagatttct ctgtaaacgt tatttctaaa tctggtacaa ctacagaacc agcagttgca
     7681 tttagattgt tcaaacaatt agttgaagaa agatacggta aagaagaagc acaaaaacgt
     7741 atatttgcaa caacggataa agaaaaaggt gctttaaaac agttggctac aaacgaaggt
     7801 tatgaaacgt ttatcgtacc tgatgatgta ggtggaagat attctgtttt aacagcagta
     7861 ggattattac caattgcaac tgctggaatt aacatcgaag ctatgatgat tggtgctgca
     7921 aaagcacgtg aagaattatc ttcagataaa ttagaagaaa acattgcata ccaatatgcg
     7981 acaattcgaa acattttata tgcaaaaggt tatacaacag aaatgttgat taactatgaa
     8041 ccatctatgc aatactttaa tgaatggtgg aaacaattat ttggtgaatc agaaggtaaa
     8101 gacttcaaag gtatctatcc ttcaagtgcc aactacacaa ctgatttaca ttctttaggt
     8161 caatatgtac aagaaggccg tcgtttctta ttcgaaacag tggtaaaagt aaatcatcct
     8221 aaatatgata ttactattga aaaagatagt gatgatctag acggattaaa ttatttggct
     8281 ggtaaaacaa tcgacgaagt taacacaaaa gcattcgaag gtacattatt agcgcatact
     8341 gatggtggtg ttcctaacat ggtagtgaac attccacaat tagatgaaga aactttcggt
     8401 tatgtcgtat acttcttcga acttgcttgt gcaatgagtg gataccaatt aggtgtaaat
     8461 ccatttaacc aacctggtgt agaagcatat aaacaaaaca tgttcgcatt attaggtaaa
     8521 cctggttttg aagacttgaa aaaagaatta gaagagcgtt tataaaatac attacttcaa
     8581 tgattagtga agtttgaaaa gatagaacta gacgttaact atttaaagca tattttcgag
     8641 gttgtcatta caaatgtaaa aatgtaatga caacctcgtt tttatttata tgcaagaact
     8701 aggttactag ctaatgtgac aagatgttaa gagaaaatta aagaaaaaat aacatctgtc
     8761 atacaataat attgttatac tactagagac tgatttatta gcatgattac atgttaatgt
     8821 ttctttactt agtaattaac tttataatgt aagaataatt atcttcaacc aaagaaaggg
     8881 attgatgatt tgtcgtttca tcaagtagaa gaatggtttg agatatttcg acagtttggt
     8941 tatttacctg gatttatatt gttatatatt agagcgataa ttccagtatt tcctttagca
     9001 ctctatattt taattaacat tcaagcttat ggacctattt taggtatatt gattagttgg
     9061 cttggattaa tttctggaac atttacagtc tatttgatct gtaaacgatt ggtgaacact
     9121 gagaggatgc agcgaattaa acaacgtact gctgttcaac gcttgattag ttttattgat
     9181 cgccaaggat taatcccatt gtttatttta ctttgttttc cttttacgcc aaatacatta
     9241 ataaattttg tagcgagtct atctcatatt agacctaaat attatttcat tgttttggca
     9301 tcatcaaagt tagtttcaac aattatttta ggttatttag gtaaggaaat tactacaatt
     9361 ttaacgcatc ctttaagagg gatattaatg ttagttgtgt tggttgtatt ttggattgtt
     9421 ggaaaaaagt tagaacagca ttttatggga tcgaaaaagg agtgacatcg tgaaaaaagt
     9481 tgtaaaatat ttgatttcat tgatacttgc tattatcatt gtactgttcg tacaaacttt
     9541 tgtaatagtt ggtcatgtca ttccgaataa tgatatgtca ccaaccctta acaaagggga
     9601 tcgtgttatt gtaaataaaa ttaaagttac atttaatcaa ttgaataatg gtgatatcat
     9661 tacatatagg cgtggtaacg agatatatac tagtcgaatt attgccaaac ctggtcaatc
     9721 aatggcgttt cgtcagggac aattataccg tgatgaccga ccggttgacg catcttatgc
     9781 caagaacaga aaaattaaag attttagttt gcgcaatttt aaagaattag atggagatat
     9841 tataccgcct aacaattttg ttgtgctaaa tgatcatgat aacaatcagc atgattctag
     9901 acaatttggt ttaattgata aaaaggatat tattggtaat ataagtttga gatattatcc
     9961 tttttcaaaa tggacgattc agttcaaatc ttaaaaagag gtgtcaaaat tgaaaaaaga
    10021 attattggaa tggattattt caattgcagt cgcttttgtc attttattta tagtaggtaa
    10081 atttattgtt acaccatata caattaaagg tgaatcaatg gatccaactt tgaaagatgg
    10141 cgagcgagta gctgtaaaca ttattggata taaaacaggt ggtttggaaa aaggtaatgt
    10201 agttgtcttc catgcaaaca aaaatgatga ctatgttaaa cgtgtcatcg gtgttcctgg
    10261 tgataaagta gaatataaaa atgatacatt atatgtcaat ggtaaaaaac aagatgaacc
    10321 atatttaaac tataatttaa aacataaaca aggtgattac attactggga ctttccaagt
    10381 taaagattta ccgaatgcga atcctaaatc aaatgtcatt ccaaaaggta aatatttagt
    10441 tcttggagat aatcgtgaag taagtaaaga tagccgtgcg tttggcctca ttgatgaaga
    10501 ccaaattgtt ggtaaagttt catttagatt ctggccattt agtgaattta aacataattt
    10561 caatcctgaa aatactaaaa attaatatga aacaaataca acatcgtttg tcggttttaa
    10621 tactgataaa cgatgtttta ttttgttagt accacaataa aagctaagtt cgaaatgaac
    10681 ttataataaa tcaatcacaa tcactttgtg ttaaaatatg tgtcaaagga agtgagggtt
    10741 tgtcatgaca ttacatgctt atttaggtag agcgggaaca ggtaagtcta cgaaaatgtt
    10801 gaccgaaata aaacaaaaaa tgaaagcaga tccgcttgga gatccaatca ttttaattgc
    10861 gccaactcaa agtacatttc aattagaaca agcctttgtc aatgatccgg aattaaatgg
    10921 tagtttaaga acagaagtgt tgcattttga acgattaagt catcgtattt tccaagaagt
    10981 tggtagttat agcgaacaaa agttatctaa agctgcaacg gaaatgatga tttataacat
    11041 tgttcaagaa caacaaaagt atttaaaact ttatcaatca caagcaaaat attatgggtt
    11101 tagtgaaaaa ttaacagaac aaattcaaga ttttaaaaaa tatgcagtaa cgcctgaaca
    11161 tttagaacac tttattgctg ataaaaatat gcaaactcga actaaaaata agttagagga
    11221 tattgcttta atataccgtg agttcgaaca acgcattcaa aacgagttta ttactggtga
    11281 ggattcatta caatatttta ttgattgtat gccgaaatca gagtggctaa aacgtgctga
    11341 tatatatatt gatggttttc acaacttttc aacgattgag tatttaataa tcaaaggatt
    11401 aattaaatat gcgaagagtg tcacaattat attgacgaca gatggtaacc acgatcaatt
    11461 tagtttattt agaaaaccat cggaagtgtt acgacatatt gaagaaatag caaatgaact
    11521 caatatttct attgaacgtc aatatttcaa ccaattatat cgcttcaata atcaagattt
    11581 aaagcatctt gaacaagaat ttgatgtact tcaaatcaat cgagtggcat gtcaaggtca
    11641 tatcaatatt ttagaatctg cgactatgag agaggaaata aatgaaattg cgcgacgtat
    11701 catcgttgat attcgtgata agcaattacg atatcaagat attgctattt tatatcgtga
    11761 tgaatcttat gcttatttat ttgattccat attaccgctt tataatattc cttataatat
    11821 tgatacaaag cgttcgatga cacatcatcc ggtcatggaa atgattcgtt cattgattga
    11881 agttattcaa tctaattggc aagtgaatcc aatgctacgc ttattgaaga ctgatgtgtt
    11941 aacggcatca tatctaaaaa gtgcatactt agttgattta cttgaaaatt ttgtacttga
    12001 acgtggtata tacggtaaac gttggttaga tgatgagcta tttaatgtcg aacattttag
    12061 caaaatgggg cgtaaagcgc ataaactgac cgaagatgaa cgtaacacat ttgaacaagt
    12121 cgttaagtta aagaaagatg tcattgataa aattttacat tttgaaaagc aaatgtcaca
    12181 agcggaaact gtaaaagatt ttgcaactgc tttttatgaa agtatggaat atttcgaact
    12241 gccaaatcaa ttgatgacag agcgagatga acttgattta aatggtaatc atgaaaaggc
    12301 ggaggaaatt gatcaaatat ggaatggctt aattcaaatc cttgatgact tagttctagt
    12361 atttggagat gaaccaatgt cgatggaacg tttcttagaa gtatttgata ttggtttaga
    12421 acaattagaa tttgttatga ttccgcaaac attggaccaa gtaagtattg gtacgatgga
    12481 tttggctaaa gtcgataata agcaacatgt tttcttagta ggtatgaatg atggtacgat
    12541 gccacaacca gtaactgcgt caagcttgat tacagatgaa gaaaagaaat actttgaaca
    12601 gcaggctaat gtcgagttaa gtccaacatc agatatttta cagatggatg aagcatttgt
    12661 ttgctatatt gctatgacaa gagcgcgaca agatgttaca ttttcttaca gtctaatggg
    12721 atcaagtggt gatgataagg agatcagccc atttttaaat caaattcaat cattgttcaa
    12781 ccaattggaa attactaaca ttcctcaata ccatgaagtt aacccattgt cactaatgca
    12841 acatgctaag caaaccaaaa ttacattatt tgaagcattg cgtgcttggt tatatgatga
    12901 aattgtggct gatagttggt tagatgctta tcaagtaatt agagatagcg atcatttaaa
    12961 tcaaggttta gattatttaa tgtcagcatt aacgtttgac aatgaaactg taaaattagg
    13021 tgaaacgttg tctaaagatt tatatggtaa ggaaatcaat gccagtgtat cccgttttga
    13081 aggttatcaa caatgcccat ttaaacacta tgcgtcacat ggtctgaaac taaatgagcg
    13141 aacgaagtat gaacttcaaa actttgattt aggtgatatt ttccattctg ttttaaaata
    13201 tatatctgaa cgtattaatg gcgattttaa acaattagac ctgaaaaaaa taagacaatt
    13261 aacgaatgaa gcattggaag aaattttacc taaagttcag tttaatttat taaattcttc
    13321 agcttactat cgttatttat caagacgcat tggcgctatt gtagaaacaa cactaagcgc
    13381 attaaaatat caaggcacgt attcaaagtt tatgccaaaa cattttgaga caagttttag
    13441 aaggaaacca agaacaaatg acgaattaat tgcacaaaca ttaacgacaa ctcaaggtat
    13501 tccaattaat attagagggc aaattgaccg tatcgatacg tatacaaaga atgatacaag
    13561 ttttgttaat atcattgact ataaatcctc tgaaggtagt gcgacacttg atttaacgaa
    13621 agtatattat ggtatgcaaa tgcaaatgat gacatacatg gatatcgttt tacaaaataa
    13681 acaacgcctt ggattaacag atattgtgaa accaggtgga ttattatact tccatgtaca
    13741 tgaacctaga attaaattta aatcatggtc tgatattgat gaagataaac tagaacaaga
    13801 tttaattaaa aagtttaagt tgagtggttt agttaatgca gaccaaactg ttattgatgc
    13861 attggatatt cgtttagaac ctaaattcac ttcagatatt gtaccagttg gtttgaataa
    13921 agatggctct ttgagtaaac gaggcagcca agtggcagat gaagcaacga tttataaatt
    13981 catccaacat aacaaagaga attttataga aacagcttca aatattatgg atggacatac
    14041 tgaagttgca ccattaaagt acaaacaaaa attgccatgt gctttttgta gttatcaatc
    14101 ggtatgtcat gtagatggca tgattgatag taagcgatat cgaactgtag atgaaacaat
    14161 aaatccaatt gaagcaattc aaaatattaa cattaatgat gaatttgggg gtgagcaata
    14221 gatgacaatt ccagagaaac cacaaggcgt gatttggact gacgcgcaat ggcaaagtat
    14281 ttacgcaact ggacaagatg tacttgttgc agccgcggca ggttcaggta aaacagctgt
    14341 actagttgag cgtattatcc aaaagatttt acgtgatggc attgatgtcg atcgactttt
    14401 agtcgtaacg tttacaaact taagcgcacg tgaaatgaag catcgtgtag accaacgtat
    14461 tcaagaggca tcgattgctg atcctgcaaa tgcacacttg aaaaaccaac gcatcaaaat
    14521 tcatcaagca caaatatcta cactccatag tttttgcttg aaattaattc aacagcatta
    14581 tgatgtatta aatattgacc cgaactttag aacaagcagt gaagctgaaa atattttatt
    14641 attagaacaa acgatagatg aggtcataga acaacattac gatatccttg atcctgcttt
    14701 tattgaatta acagagcaat tgtcttcaga tagaagtgat gatcagtttc gaatgattat
    14761 taaacaattg tatttcttta gcgttgcaaa tccaaatcct acaaattggt tggatcaatt
    14821 ggtgacacca tacgaagaag aagcacaaca agcgcaactt attcaactac taacagactt
    14881 atctaaagta tttatcacag ctgcttatga tgctttaaat aaggcgtatg atttgtttag
    14941 tatgatggat agcgtcgata aacatttagc tgttatagaa gatgaacgac gtttaatggg
    15001 gcgtgtttta gaaggtggct ttattgatat accttattta actggtcacg aatttggcgc
    15061 gcgtttgcct aatgtaacag cgaaaattaa agaagcaaat gaaatgatgg tcgatgcctt
    15121 agaagatgct aaacttcagt ataaaaaata taaatcatta attgataaag tgaagagtga
    15181 ttacttttca agagaagctg atgatttgaa agctgatatg caacaattgg cgccacgagt
    15241 aaagtacctt gcgcgtattg tgaaagatgt tatgtcagaa ttcaatcgaa aaaagcgtag
    15301 caaaaatatt ttggattttt ctgattatga acattttgca ttacaaattt taacaaatga
    15361 ggatggttcg ccttcagaaa ttgccgaatc ataccgtcaa cacttccaag aaatattggt
    15421 cgatgagtat caagatacga accgagttca agagaaaata ctatcttgca tcaaaacggg
    15481 tgatgaacat aatggtaatt tatttatggt tggagatgtt aagcaatcca tttataaatt
    15541 tagacaagct gatccaagtt tatttattga aaagtatcaa cgctttacta tagatggaga
    15601 tggcactgga cgtcgaattg atttgtcgca aaacttccgt tctcgaaaag aagtactgtc
    15661 aacgactaac tatatattca aacatatgat ggatgaacaa gtcggtgaag taaaatatga
    15721 tgaagcggca cagttgtatt atggtgcacc atatgatgaa tcggaccatc cagtaaactt
    15781 aaaagtcctt gttgaagcgg atcaagaaca tagtgattta actggtagtg aacaagaagc
    15841 gcattttata gtagaacaag ttaaagatat cttagaacat caaaaagttt atgatatgaa
    15901 aacaggaagc tatagaagtg cgacatacaa ggatatcgtt attctagaac gcagctttgg
    15961 acaagctcgc aatttacaac aagcctttaa aaatgaagat attccattcc atgtgaatag
    16021 tcgtgaaggt tactttgaac aaacagaagt ccgcttagta ttatcatttt taagagcgat
    16081 agataatcca ttacaagata tttatttagt tgggttaatg cgctccgtta tatatcagtt
    16141 caaagaagac gaattagctc aaattagaat attgagtcca aatgatgact acttctatca
    16201 atcgattgta aattacatta atgacgaagc agcagatgca attttagttg ataaattaaa
    16261 aatgttttta tcagatattc aaagttacca acaatatagt aaagatcatc cggtgtatca
    16321 gttaattgat aaattttata atgatcatta tgttattcaa tactttagtg gacttattgg
    16381 tggacgtgga cgacgtgcaa atctttatgg tttatttaat aaagctatcg agtttgagaa
    16441 ttcaagtttt agaggtttat atcaatttat tcgttttatc gatgaattga ttgaaagagg
    16501 caaagatttt ggtgaggaaa atgtagttgg tccaaacgat aatgtcgtta gaatgatgac
    16561 aattcatagt agtaaaggtc tagagtttcc atttgtcatt tattctggat tgtcaaaaga
    16621 ttttaataaa cgtgatttga aacaaccagt tattttaaat cagcaatttg gtctcggaat
    16681 ggattatttt gatgtggata aagaaatggc atttccatct ttagcttcgg ttgcatatag
    16741 agctgttgcc gaaaaagaac ttgtgtcaga agaaatgcga ttagtctatg tagcattaac
    16801 aagagcgaaa gaacaacttt atttaattgg tagagtgaaa aatgataaat cattactaga
    16861 actagagcaa ttgtctattt ctggtgagca cattgctgtc aatgaacgat taacttcacc
    16921 aaatccgttc catcttattt atagtatttt atctaaacat caatctgcgt caattccaga
    16981 tgatttaaaa tttgaaaaag atatagcaca agttgaagat agtagtcgtc cgaatgtaaa
    17041 tatttcaatt atatactttg aagatgtgtc tacagaaacc attttagata ataatgaata
    17101 tcgttcggtt aatcaattag aaactatgca aaatggtaat gaggatgtta aagcacaaat
    17161 taaacaccaa cttgattatc aatatccata tgtaaatgat actaaaaagc catcaaaaca
    17221 atctgtttct gaattgaaaa ggcaatatga aacagaagaa agtggcacaa gttacgaacg
    17281 agtaagacaa tatcgtatcg gtttttcaac gtatgaacga cctaaatttc taagtgaaca
    17341 aggtaaacga aaagcgaatg aaattggtac gttaatgcat acagtgatgc aacatttacc
    17401 attcaaaaaa gaacgcatat ctgaagttga gttacatcag tatatcgatg gattaatcga
    17461 taaacatatt atcgaagcag atgcgaaaaa agatatccgt atggatgaaa taatgacatt
    17521 tatcaatagt gagttatatt cgattattgc tgaagcagag caagtttatc gtgaattacc
    17581 gtttgtagtt aaccaagcat tagttgacca attgccacaa ggagacgaag acgtctcaat
    17641 tattcaaggt atgattgact taatctttgt taaagatggt gtgcattatt ttgtagacta
    17701 taaaaccgat gcatttaatc gtcgccgtgg gatgacagat gaagaaattg gtacacaatt
    17761 aaaaaataaa tataagatac agatgaaata ttatcaaaat acgcttcaaa cgatacttaa
    17821 taaagaagtt aaaggttatt tatacttctt caaatttggt acattgcaac tgtagtattt
    17881 tgattttcaa aagaataaaa aataatttcg attaagtgca aagcccttgt agcagaatga
    17941 acataactca ttttcaaaat tgtcttactt atttatttgt tatttgataa cgaaaaaagt
    18001 tataatgtga attaagataa agatgaggag ttgagaatga atgaaattct tatcattcaa
    18061 gtataatgac aaaacttcat atggcgttaa agtaaaacgc gaagatgctg tatgggattt
    18121 aacacaagta tttgctgact ttgcagaagg agatttccat cctaaaacat tgttagctgg
    18181 tttacaacaa aatcatactt tagattttca agaacaagta cgtaaagcag ttgtagcagc
    18241 agaagatagc ggcaaagctg aagactataa aatttcattt aatgacattg aattcttacc
    18301 accagtaaca cctccgaata atgtgattgc ttttggtaga aattacaaag atcatgcgaa
    18361 cgaattaaat catgaagtag aaaaattata tgtatttaca aaagcagcgt catctttaac
    18421 aggagataat gcaacaattc caaatcataa agatattact gatcaattag attatgaagg
    18481 tgaattaggt attgttattg gtaagtctgg tgaaaagatt ccaaaagcat tagctttaga
    18541 ttatgtttac ggctatacaa ttattaacga tatcactgat cgcaaagcac aaagtgaaca
    18601 agatcaagca tttttatcaa aaagtttaac tggcggttgc ccaatgggtc cttatatcgt
    18661 tactaaagac gaactaccat tacctgaaaa tgtaaatatt gttacaaaag ttaacaatga
    18721 aattagacaa gatggtaaca ctggcgaaat gattcttaaa attgatgaat taatagaaga
    18781 aatttcaaaa tatgttgcac tacatccggg agatattatt gcaactggta caccagcagg
    18841 cgttggtgca ggtatgcaac cacctaaatt tttacaacca ggtgatgaag ttaaagtgac
    18901 tattgataat attggaacgc tgacaactta tatcgctaaa taattatcat ttaaaaagct
    18961 aaccagtctt tatatagatt ggttagtttt ttcttgcttt tctaaaaagg tgttaaagat
    19021 aaattattta taatgttacc attttgagat gaaagtgaaa tattgatatt aagaagtagt
    19081 tgattatttt acagcagatt cacaatattc taataaggaa taaaatgtca tgttcttcct
    19141 ctcaaatata gaagtgtggt agaatatata ttcgtgtata atcaaatcat gattaaatta
    19201 caagcaagtg ggtattaatc ccaagaagct ttaatttaat aatataaata attaaagtgg
    19261 ggggaatcag tatgttacat ttacatatat taagttgggt attagcgatt attttattta
    19321 tcgctacata cttaaacatt tcaaaaaatc aaggcggatc accatttttc aaaccgttgc
    19381 acatgatttt acgcttattt atgctgttga cgttaatttc aggattttgg atattaattc
    19441 agtcatttat gaatggcggg gcaaatcata tgttgcttac attgaaaatg ctgtgtggtg
    19501 ttgcagtagt tggattgatg gaagtgtcga ttgctaaaag aaagagacat gaacaaagtc
    19561 acaaaatgtt ttggataaca atggcattaa ttatcatcac aatggtatta ggtgtcattc
    19621 taccgttagg gcctatatca aaattattcg gtattggcta attgataaga catatagtat
    19681 atgacataag gcatggacaa ttattagtcc aagaccttat gtcttttttg attctaaaaa
    19741 tgtttaaaca taaaaaaagg actggatagt agtaccagtc caacataatt ctaacctaga
    19801 caattaagat tagattgaat cgcatgattc atttatttag ctttgtaacc aatcatattg
    19861 attaaatctt tagggtggct atatggtggt gcataagcca cttcaaactc agttaactca
    19921 tctacagtta gctggttcat cattgccatc gatagtacat caatacgttt atctgcacct
    19981 tcttttccta ctgcagctgc tcttaaaatc tgacggtttg aagtgtcata atataccctt
    20041 aagtgtaaag gggaatttcc tgggtaataa ttcgcgtgtg caccttgagt gacttccacc
    20101 attttatagt caaattgctt tagttcattt ggtttaacgc cgacactcgc aaatgtataa
    20161 tcaaagaact tcacaatatt gttgcctaag aagcctttga attcaatagt gtcatttcca
    20221 gcaatttgtt cggcaacaat acttgctgca cggtgagcgc cccaagctaa aggaacacta
    20281 gccggtagat cgacatgtcg ataatgtgat gttgcaatat cgcctattgc ataaatgttt
    20341 ggaacatttg tttcaaattt atcgtttacc ggtatgaaac cttttcgatc aagtttgata
    20401 tttgaacttt cgataaattt tgaattgggg tgagtaccga caccttcaat aatcatatcg
    20461 taatgttcaa cttttcctga tttaaatgta atttcatttc cattgatagc atcaatttcc
    20521 tcatttaaac ggtatggaat ctcccgctta tctaattcat caagtatagg ttgattcatg
    20581 tcagcatcca ttaatttatt tatcttatca gatcgatgaa ttaaagtagg gtgtaaacca
    20641 cgttcgtaaa gattttcaag aacttctaat gaaacatacc ctgcacctac aactaatact
    20701 ttatcaactt gatttgcttt gatgaattga tcgatagcat cagtgtcttc taaatttcta
    20761 agtgtaaatg taatatcact ttcaaagcca aggctatttg cacttgcacc agggcttaaa
    20821 atgagtttat cgtaagattc ttcaaattgt tcgttggtct ttctatttaa tacagttaca
    20881 gtttgtcttt catcattgat tgcaataact tcatgataag tttttactgt aatttgcttt
    20941 ctatcataaa atttttcagg tgtatacgct aaagcatatt ttctatcttc aacaacttcg
    21001 ccaatgacat aaggcaatgc acaattagca aagctcatat cacgatcttt ttcaaaaata
    21061 ataatgtcac tttctttatc taaacgtcga atttggctgg cacatgttgc accgccagcg
    21121 actgctccga ctacgactat tttgggcatc gtaacttacc tcctattgtt ttaaaatatt
    21181 ataaaatgat tagttataaa ttaacagtaa tatctaatat ttaaattaaa gaaatcattc
    21241 aaatatcgac caatgccatc ttcattattg ttgaatgtaa tattgttcgc tacatcttta
    21301 agttcttgca aaccattttc catagcaaca ccatggcggg cgtactcaat catttcaata
    21361 tcattatctt catcaccgaa tgcaataata ttatttcggt caatatttaa aaattgtcta
    21421 acttgctcaa tgcctcttgc tttattaata ccaagtttta caatttcaat gacagggaat
    21481 ggtgcgcccc agcgtcgatg ctcaatatga tcggcataaa aatgagtaag catatttttg
    21541 atttcaggta ttttactttc ttcggcttca attaaaattg aggtagggga ttctttcaag
    21601 tggacaagta aattaccagt ttgaattctt ggattaccca ttgaaaaacc ttcaaataat
    21661 cttggatcat gattgttaat gaaaacataa tctttcactt ctgctataat attcgatact
    21721 tgatattgtt gtaatccttg aataatgttt tgtgcgatgc ctaaatctaa aatttcatgg
    21781 caagttttga agtttttatc tttagggtga tgtacgtaag cgccattaaa attaacaatt
    21841 ggtgtcgtta aatttaattc atgataatac atttgacttg cacgataagg tctgccagtc
    21901 gcaatcataa tttggtgtcc acgttgttgt aattcattta atacttgttt agtatatgat
    21961 gaaatttctt tgttatcgtt taataatgtt ccgtctaagt ctagacatat taaatgtggt
    22021 tgcatatctc tctcctcata agttgcttag ttcatataga tacatcatag cattttattg
    22081 gcgttttgtc ggatatttga tatattatga ataatatgac attaaagagg tgatatggat
    22141 ggaagaggca ttgaaagata gtatcttagg tgcattagaa atggtaattg accctgaatt
    22201 aggaattgat atcgttaatt taggtttagt atacaaagtg aatgttgatg atgaaggcgt
    22261 atgtacagtt gatatgactt taacatcaat gggatgtcca atgggacctc aaattattga
    22321 tcaagttaaa acagtattag cagagattcc tgaaatacag gatactgaag tgaatatcgt
    22381 atggagtcca ccttggacaa aagatatgat gtcacgttac gctaagattg cacttggtgt
    22441 gagctaacat aaacgtgcat tgcctgtaga atttctttta gaaattctct gtgttgggtc
    22501 cccgcagtca atataacatt gtagagtata attcattgtg aataagctcc cttcaaagta
    22561 gacattgaaa aatgaaaatt ttgaagggag ccttatttat gttctaggtt ctgatttcat
    22621 tttgaaaatt tatatctaac ttgtttcaaa aaatatgaat taagacagtg acttgcactt
    22681 ctaagagtgc gcaattcatt ttctagttga atattttctt gaaaattttt ataataaatt
    22741 gaatttatat tacagttata ttacaataca taatacttca tagtttttaa gttgccgagt
    22801 tgcattacaa attggatttc gaagtatgaa tcatcattgg taattttttt aatttaacag
    22861 tattttagtc aatatgattt tgatgatgtc gtaattcaat aatatacata tgcatttcaa
    22921 tattctttta aacagcataa ctaaatttaa ttaagcatct aagaagaaca gttttaaaag
    22981 ggaagaatgc aaaaatgaac aaaacaaagg gttttacaaa gtataagaaa atgagatata
    23041 tgccagggct cgatggtttg agggcaatcg ctgttctagg aattattatt taccacttaa
    23101 acaagcaatg gttgacaggt ggctttttag gtgtggatac attttttgtg atctctggtt
    23161 atttaattac aagcttatta ctcaaagagt atgatgacac aggtatcatt aaattgaaaa
    23221 gcttttggat acgtcgttta aaacgtttat taccagcagt catagtttta ttaatggttg
    23281 tagggacagc aaccttatta ttaaaatcag ataatatcat tagggttaaa catgatatta
    23341 ttgctgcgat attttatgta tcaaactggt ggtatatagc aaaagatgtt aattattttg
    23401 agcaattttc atttatgcca ttaaagcatt tatggtcttt agcaattgaa gaacagtttt
    23461 acatattttt cccagttatt ttggttacat tattgttaac aattaaaaag cgatacaaaa
    23521 taggatttat tttttgggga gtatcaataa tttctttagg gttaatgatg tttatctaca
    23581 gtattaatgg ggatcattca cgagtgtatt ttggtacaga tactagatta cagacattgt
    23641 tactgggtgt tattttagct tttttatggc caccgtttaa attgaaaaat gatccaccta
    23701 aagttgtaaa atatgttatt gatagcatag gtagtttatc atttatagta cttatattat
    23761 tatttttcat tattaatgat gagacgaatt ggatatatga tggtggtttc tatttaatat
    23821 ccatattaac gttatttatt attgccagtg tcgttcatcc atctacatgg atagcgaaga
    23881 tattttcaaa tccagtgtta gtatttatcg ggaaaaggtc ttatagttta tatttatggc
    23941 attttgcagt aattagtttc gtacatagtt actatgtaga tggacagata cctgtatatg
    24001 tgtactttat agatataagt ttaacaatta tatttgcaga gctatcatat cgctttatag
    24061 aaactccatt tagaaaagaa ggtattaaag ctttaaattg gcgaccttct tatataccac
    24121 aatttataag aatggcaatt gtagtaacct tgttaattcc atttatgttg attttagtag
    24181 gtgcattcaa taaatatggt aaagacatta ttggagaaaa agcgaatagc tttgatacca
    24241 ctattgaaga taattattta atgcggatag caccaattga taacattcac attgatggct
    24301 tagtaagtga gaagaaaaag gaatcttccg acgtatataa taatattaaa cctcttttaa
    24361 tcggtgattc agtaatggtt gatatcggtg agtcatttaa gtcatcagtt cctaagtcta
    24421 gaattgatgg aaaagtaggg cgtcaattgt atcaaacctt acctttagtt aaagcgaatt
    24481 attcacaata taaaaaatca tctgatcaag tcgtattaga attaggtaca aatggcgact
    24541 ttactgtcaa acagctcgac gatttactta atcaatttgg aaaagccaag atttatttag
    24601 ttaatacacg tgttccaaga atttatgagg caaatgtaaa tcgattatta gctgacgcgg
    24661 cgaaacgaaa gtccaatgtc acattaattg attggaataa gcgatcacaa ggacatagtg
    24721 aatattttgc accagacggt gtacatttag agtacaaagg agtcctagct ttaaaagatg
    24781 aaatattaaa agcacttaaa aagaaataac atatgtttaa taagtctagt taatgtgtaa
    24841 cgtaacatta gctagatttt tttattcaaa aaaatattta caaatattag gaaatttaag
    24901 tgtaaaagag ttgataaatg attatattgg gactataata taattaaggt caaagaaagt
    24961 caaagtcaaa agtgcaactt gaccaactct acaaattaga ggtgaaatta tatggatata
    25021 aataaaatga catatgctgt tcaaagtgct ttacaacaag cagttgaact gagtcagcaa
    25081 cataaattac aaaatataga aattgaggca attttaagcg ctgccttaaa tgaaagtgaa
    25141 agcttatata aaagtatttt agaacgagca aatattgagg tagatcaatt aaacaaagct
    25201 tatgaagaca aactaaacac gtatgcatct gtagaaggtg acaatataca atatggtcaa
    25261 tatattagcc aacaagcaaa ccaattgata actaaggctg aatcatacat gaaagaatat
    25321 gaagatgaat atatttcaat ggagcatatt ttacgttcgg caatggacat tgatcaaaca
    25381 acaaaacatt atataaataa taaagtagaa gttatcaaag aaattattaa aaaagtaaga
    25441 gggggaaatc acgtgacatc acaaaatcca gaagttaatt acgaagcatt agctaaatat
    25501 ggccgcgact tagtagaaga agttagacaa ggtaaaatgg atcctgttat aggaagagat
    25561 gaagaaattc gaaatacgat tcgtatttta agtcgtaaaa ctaaaaacaa ccctgtgctc
    25621 attggtgaac caggtgttgg taaaactgca attgttgaag gattagcgca acgtatagtt
    25681 aagaaagatg tgccagaatc attattagat aaaactgttt ttgagttaga tttaagcgca
    25741 ttagtagcgg gcgctaaata tcgtggtgaa tttgaagaga gattaaaagc agtcctaaaa
    25801 gaagttaaag agtctgatgg tagaattata ttatttattg atgaaatcca tatgcttgta
    25861 ggtgctggta aaacagatgg tgccatggat gcaggcaaca tgctaaaacc aatgttagca
    25921 cgaggagagt tacattgtat tggtgcaaca actttaaatg aatatcgaga atatattgaa
    25981 aaagattcgg cattagagcg tcgtttccaa aaagtagcag ttagtgagcc tgatgttgaa
    26041 gatacaattt caattttacg tggtttaaaa gaacgatatg aagtgtatca tggtgtgcgt
    26101 attcaagata gagccttagt tgctgccgct gaattgtctg atcgttacat cactgatcgt
    26161 tttttaccag ataaagcgat tgatttagtt gaccaagcat gtgcaacaat tcgtacggaa
    26221 atgggatcaa atccaactga attggatcaa gttaatagac gtgtcatgca attagaaatt
    26281 gaagaaagcg cacttaaaaa tgaatctgac aatgcgagca aacagagatt acaagaacta
    26341 caagaagagc ttgccaatga aaaagagaaa caagcagcac ttcaatctcg tgtagaatca
    26401 gaaaaagaaa aaatagcaaa tttacaagaa aaacgtgcgc aactagatga aagtagacaa
    26461 gcgttggaag atgcacaaac aaataacaat ttagaaaaag ctgctgaact acaatatgga
    26521 acaattcctc aattggaaaa agaacttaga gaattagagg ataatttcca agatgagcaa
    26581 ggtgaagata cagatcgaat gattcgtgaa gttgtaacag acgaagaaat tggcgatatt
    26641 gtcagccaat ggacaggcat accagtttca aaattagttg aaacagaacg tgaaaaatta
    26701 cttcacttaa gtgacatctt gcataaacgt gttgtaggtc aagataaagc ggttgacctg
    26761 gtttcagatg cagtagttag agcaagagca ggtattaaag atccaaacag acctattggt
    26821 agtttcttat tcctaggtcc aactggagta ggtaaaactg aattagctaa atcattagct
    26881 gcatcattat ttgattctga aaaacatatg attcgtattg atatgagtga atatatggaa
    26941 aaacatgcag tatcaagatt gataggggca cctccaggat atattggaca tgatgaaggg
    27001 ggtcaattaa ctgaagcggt tcgtcgtaat ccatactcag ttattttatt agatgaggtt
    27061 gaaaaagcgc atactgacgt ctttaatgta ttattgcaaa ttttagatga aggccgttta
    27121 actgattcta aaggacgtag cgttgatttt aaaaatacta ttattattat gacaagtaat
    27181 attggatctc aagttttatt agaaaacgta aaagagactg gtgaaattac agaatcaaca
    27241 gaaaaagctg ttatgacaag tttaaatgca tatttcaaac cagaaatttt gaatcgtatg
    27301 gatgatatcg tattatttaa accattatct attgatgaca tgagtatgat tgtagataaa
    27361 atcttaacgc aattaaatat aagattatta gaacaacgaa tctcaattga agtttctgat
    27421 gatgctaaag cttggctagg tcaagaagct tatgaacctc aatacggtgc aagaccatta
    27481 aaacgttttg tacaacgcca aattgaaaca ccattagcac gtatgatgat taaagaggga
    27541 ttcccagaag gtacaacgat taaagttaat ttaaattcag acaataactt aacgtttaat
    27601 gttgaaaaaa ttcatgaata aaattgaacc aagaatgtga tgattaatcg cattcttggt
    27661 tttttagatt tgaaatttat tataattgtt cattggcaat atacttttta aatgatttaa
    27721 taaacaccaa tatttctggg ctttcttttt tgctgtatat atatgtaaat gaaataggtg
    27781 cttgtaaaat tttcgtgtta ataaccgaaa tattgtaatc actatttgtt gttatataaa
    27841 gaggcaggaa tgatatacct tgattcattt cgattaa
//
LOCUS       DBNENW010000017        27763 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNENW010000017 DBNENW010000000
VERSION     DBNENW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.017"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            280..588
                     /gene="rpsJ"
                     /locus_tag="AC1LW8_000580"
     CDS             280..588
                     /gene="rpsJ"
                     /locus_tag="AC1LW8_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIP4772445.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            616..1278
                     /gene="rplC"
                     /locus_tag="AC1LW8_000581"
     CDS             616..1278
                     /gene="rplC"
                     /locus_tag="AC1LW8_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIP4772446.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1305..1928
                     /gene="rplD"
                     /locus_tag="AC1LW8_000582"
     CDS             1305..1928
                     /gene="rplD"
                     /locus_tag="AC1LW8_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIP4772447.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            1928..2203
                     /gene="rplW"
                     /locus_tag="AC1LW8_000583"
     CDS             1928..2203
                     /gene="rplW"
                     /locus_tag="AC1LW8_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIP4772448.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2236..3069
                     /gene="rplB"
                     /locus_tag="AC1LW8_000584"
     CDS             2236..3069
                     /gene="rplB"
                     /locus_tag="AC1LW8_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIP4772449.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3136..3414
                     /gene="rpsS"
                     /locus_tag="AC1LW8_000585"
     CDS             3136..3414
                     /gene="rpsS"
                     /locus_tag="AC1LW8_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIP4772450.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            3443..3796
                     /gene="rplV"
                     /locus_tag="AC1LW8_000586"
     CDS             3443..3796
                     /gene="rplV"
                     /locus_tag="AC1LW8_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIP4772451.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            3820..4473
                     /gene="rpsC"
                     /locus_tag="AC1LW8_000587"
     CDS             3820..4473
                     /gene="rpsC"
                     /locus_tag="AC1LW8_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIP4772452.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            4476..4910
                     /gene="rplP"
                     /locus_tag="AC1LW8_000588"
     CDS             4476..4910
                     /gene="rplP"
                     /locus_tag="AC1LW8_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIP4772453.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            4900..5109
                     /gene="rpmC"
                     /locus_tag="AC1LW8_000589"
     CDS             4900..5109
                     /gene="rpmC"
                     /locus_tag="AC1LW8_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIP4772454.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5133..5396
                     /gene="rpsQ"
                     /locus_tag="AC1LW8_000590"
     CDS             5133..5396
                     /gene="rpsQ"
                     /locus_tag="AC1LW8_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIP4772455.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            5428..5796
                     /gene="rplN"
                     /locus_tag="AC1LW8_000591"
     CDS             5428..5796
                     /gene="rplN"
                     /locus_tag="AC1LW8_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIP4772456.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            5832..6149
                     /gene="rplX"
                     /locus_tag="AC1LW8_000592"
     CDS             5832..6149
                     /gene="rplX"
                     /locus_tag="AC1LW8_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIP4772457.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6176..6715
                     /gene="rplE"
                     /locus_tag="AC1LW8_000593"
     CDS             6176..6715
                     /gene="rplE"
                     /locus_tag="AC1LW8_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIP4772458.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            6738..6923
                     /locus_tag="AC1LW8_000594"
     CDS             6738..6923
                     /locus_tag="AC1LW8_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIP4772459.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            6955..7353
                     /gene="rpsH"
                     /locus_tag="AC1LW8_000595"
     CDS             6955..7353
                     /gene="rpsH"
                     /locus_tag="AC1LW8_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIP4772460.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            7378..7914
                     /gene="rplF"
                     /locus_tag="AC1LW8_000596"
     CDS             7378..7914
                     /gene="rplF"
                     /locus_tag="AC1LW8_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIP4772461.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            7945..8304
                     /gene="rplR"
                     /locus_tag="AC1LW8_000597"
     CDS             7945..8304
                     /gene="rplR"
                     /locus_tag="AC1LW8_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIP4772462.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8325..8825
                     /gene="rpsE"
                     /locus_tag="AC1LW8_000598"
     CDS             8325..8825
                     /gene="rpsE"
                     /locus_tag="AC1LW8_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIP4772463.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            8842..9021
                     /gene="rpmD"
                     /locus_tag="AC1LW8_000599"
     CDS             8842..9021
                     /gene="rpmD"
                     /locus_tag="AC1LW8_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIP4772464.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9038..9478
                     /gene="rplO"
                     /locus_tag="AC1LW8_000600"
     CDS             9038..9478
                     /gene="rplO"
                     /locus_tag="AC1LW8_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIP4772465.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            9478..10770
                     /gene="secY"
                     /locus_tag="AC1LW8_000601"
     CDS             9478..10770
                     /gene="secY"
                     /locus_tag="AC1LW8_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIP4772466.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            10787..11434
                     /locus_tag="AC1LW8_000602"
     CDS             10787..11434
                     /locus_tag="AC1LW8_000602"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIP4772467.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            11627..11845
                     /gene="infA"
                     /locus_tag="AC1LW8_000603"
     CDS             11627..11845
                     /gene="infA"
                     /locus_tag="AC1LW8_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIP4772468.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            11877..11990
                     /gene="rpmJ"
                     /locus_tag="AC1LW8_000604"
     CDS             11877..11990
                     /gene="rpmJ"
                     /locus_tag="AC1LW8_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIP4772469.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            12013..12378
                     /gene="rpsM"
                     /locus_tag="AC1LW8_000605"
     CDS             12013..12378
                     /gene="rpsM"
                     /locus_tag="AC1LW8_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIP4772470.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            12402..12791
                     /gene="rpsK"
                     /locus_tag="AC1LW8_000606"
     CDS             12402..12791
                     /gene="rpsK"
                     /locus_tag="AC1LW8_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIP4772471.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            12866..13810
                     /locus_tag="AC1LW8_000607"
     CDS             12866..13810
                     /locus_tag="AC1LW8_000607"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIP4772472.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            13827..14195
                     /gene="rplQ"
                     /locus_tag="AC1LW8_000608"
     CDS             13827..14195
                     /gene="rplQ"
                     /locus_tag="AC1LW8_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIP4772473.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            14733..15542
                     /locus_tag="AC1LW8_000609"
     CDS             14733..15542
                     /locus_tag="AC1LW8_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIP4772474.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFSIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQTITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVGEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTAIEIFDHAEELTTIGLDLPFPIKINQMLGYQTSFLTYEGLVDQL"
     gene            15539..16399
                     /locus_tag="AC1LW8_000610"
     CDS             15539..16399
                     /locus_tag="AC1LW8_000610"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIP4772475.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            16389..17195
                     /locus_tag="AC1LW8_000611"
     CDS             16389..17195
                     /locus_tag="AC1LW8_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP4772476.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            17200..18003
                     /gene="truA"
                     /locus_tag="AC1LW8_000612"
     CDS             17200..18003
                     /gene="truA"
                     /locus_tag="AC1LW8_000612"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIP4772477.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTATAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            18243..18680
                     /gene="rplM"
                     /locus_tag="AC1LW8_000613"
     CDS             18243..18680
                     /gene="rplM"
                     /locus_tag="AC1LW8_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIP4772478.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            18700..19092
                     /gene="rpsI"
                     /locus_tag="AC1LW8_000614"
     CDS             18700..19092
                     /gene="rpsI"
                     /locus_tag="AC1LW8_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIP4772479.1"
                     /translation="MAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFESL
                     ILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKRAG
                     LLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            19439..19621
                     /locus_tag="AC1LW8_000615"
     CDS             19439..19621
                     /locus_tag="AC1LW8_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772480.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSMYLMIIFL
                     ICLGTASIIYNYKNNR"
     gene            20068..21732
                     /gene="alsS"
                     /locus_tag="AC1LW8_000616"
     CDS             20068..21732
                     /gene="alsS"
                     /locus_tag="AC1LW8_000616"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HIP4772481.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRAHIDEETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            21769..22473
                     /gene="budA"
                     /locus_tag="AC1LW8_000617"
     CDS             21769..22473
                     /gene="budA"
                     /locus_tag="AC1LW8_000617"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIP4772482.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(22860..23285)
                     /locus_tag="AC1LW8_000618"
     CDS             complement(22860..23285)
                     /locus_tag="AC1LW8_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HIP4772483.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(23474..25921)
                     /locus_tag="AC1LW8_000619"
     CDS             complement(23474..25921)
                     /locus_tag="AC1LW8_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HIP4772484.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSIDKYQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFEATENEAKKLLSEMKTESDRKYLWE
                     NSKDLDTKSADMTRTYRNIEKIAEAMKHKDTKLKIDENKKKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLNDQFSNDEKKKYTAPIK
                     TFAPESDKILSSVGQPEQAKGGNLVDIAKVKLLESIIEEDKDITKNSIDAFNKVFTYV
                     QSNATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDAMDDSTK
                     AKYKQIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSVF
                     LESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSNT
                     DSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSDSTQTFIIN
                     NTKVEVKAKGMFILKKKDDKTYECSFYNPESTNTASDIESKISMTGYSITNKNTSTSN
                     ESGVRFELQQTLNKDDN"
     gene            complement(25918..26085)
                     /locus_tag="AC1LW8_000620"
     CDS             complement(25918..26085)
                     /locus_tag="AC1LW8_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772485.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            26330..26746
                     /locus_tag="AC1LW8_000621"
     CDS             26330..26746
                     /locus_tag="AC1LW8_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP4772486.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            26816..27664
                     /locus_tag="AC1LW8_000622"
     CDS             26816..27664
                     /locus_tag="AC1LW8_000622"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4772487.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
BASE COUNT         9785 a         4410 c         5246 g         8322 t
ORIGIN      
        1 atttacaata aagaaggaaa taaaaaatat ctcgaaaata gttgaactga ctaagtatat
       61 aaagtaaaat ataaaagtat gtgttagaca gatagaaaaa ttatgtgaaa agccttgatt
      121 tatcgatgtt tttcgtgtaa tataactaac gttggacttt aagaaaagcg atgaagcgaa
      181 aggttactga cacacccggc cgctttgcca tggcgctgtg taagatagtt ttcgtggaga
      241 agtctatcac taaatgtaga cgaataagga gggaaaatta tggcaaaaca aaaaatcaga
      301 atcagattaa aagcttatga tcaccgcgtg attgatcaat cagcagagaa gattgtagaa
      361 acagcgaaac gttctggtgc agatgtttct ggaccaattc cgttaccaac tgagaaatca
      421 gtttacacaa tcatccgtgc cgtgcataag tataaagatt cacgtgaaca attcgaacaa
      481 cgtacacaca aacgtttaat cgatattgta aacccaacac caaaaacagt tgacgcttta
      541 atgggcttaa acttaccatc tggtgtagac atcgaaatca aattataata gacaatttta
      601 ggaggtggac tttcgatgac caaaggaatc ttaggaagaa aaattgggat gacacaagta
      661 ttcggagaaa acggtgaatt aatccctgta acagtagtag aagctaaaga aaatgttgta
      721 ttacaaaaga aaactgtaga agttgatgga tacaacgcaa tccaagttgg atttgaagac
      781 aaaaaagcat acaaaaaaga tgcaaaatct aataaatatg ctaataaacc agctgaaggt
      841 cacgctaaaa aagctgacgc agcacctaag cgcttcattc gtgaattccg caatgtagac
      901 gtggatgctt acgaagtagg tcaagaagtc tcagtagata cttttgtagc tggcgacgtt
      961 attgacgtaa caggcgtatc aaaaggtaaa ggtttccaag gtgcaattaa acgccacgga
     1021 caatctcgtg gacctatgtc acacggttct catttccaca gagcaccagg ttctgtaggt
     1081 atggcttcag atgcttctag agtatttaaa ggccaaaaaa tgccaggacg tatgggtgga
     1141 aacactgtaa ctgttcaaaa cttagaagta gttcaagttg acacagaaaa caaagttatc
     1201 ttagtaaaag gtaacgtacc tggacctaaa aaaggtttag tagaaatcag aacttcaatt
     1261 aaaaaaggta ataaataata agaagtgaaa ggaggaaatg cataatggct aattatgatg
     1321 ttttaaaatt agacggaact aaatcaggtt caatcgaatt aagcgatgca gtattcggta
     1381 ttgagccaaa taatagcgtt ttattcgaag ctattaattt acaacgtgct tcattacgtc
     1441 aaggtacgca tgctgttaag aatcgttcag cagtaagcgg tggcggacgt aaaccatgga
     1501 agcaaaaagg aacaggtcgt gctcgtcaag gtacaatccg tgctccacaa tggcgtggcg
     1561 gtggtatcgt attcggacca actccaagaa gttatgcata caaaatgcct aagaaaatgc
     1621 gtcgtttagc tttacgctca gcattatctt tcaaagctca agagaatggc ttaactgtag
     1681 ttgacgcatt caacttcgaa gctccaaaaa ctaaagaatt caaaaatgta ttatctacat
     1741 tagaacaacc taaaaaagta ttagtagtta ctgaaaacga agatgtaaat gttgaattat
     1801 cagcacgcaa catccctggc gttcaagtga caactgctca aggtttaaat gttttagata
     1861 tcactaatgc tgacagctta gtaattactg aagctgctgc taaaaaagtt gaggaggtgc
     1921 tcggataatg gaagcaagag atattcttaa gcgccccgta atcactgaga aatcttctga
     1981 agcaatggct gaagacaaat acactttcga cgttgatact cgtgttaaca aaacacaagt
     2041 aaaaatggct gttgaagaaa tcttcaacgt aaaagttgca agtgttaata tcatgaatta
     2101 caaacctaag aaaaaacgta tgggccgtta ccaaggctat acaaacaaaa gaagaaaagc
     2161 gattgtaact cttaaagaag gatcaatcga cttatttaac taataaaaca attaccatta
     2221 aggaggtaag cgacaatggc tattaaaaag tataagccaa taacaaatgg tcgtcgtaat
     2281 atgacttcgt tagatttcgc agaaatcacg aaaactacac ctgaaaagtc attattaaaa
     2341 ccgctaccga aaaaagcggg acgtaacaac caaggtaaat tgactgtaag acaccatggt
     2401 ggtggacaca aacgtcaata ccgtgttatc gatttcaaac gtaacaaaga tggtatcaat
     2461 gcaaaagttg attctattca atatgatcca aaccgctcag caaacatcgc tttagttgta
     2521 tatgcagacg gtgaaaaacg atatatcatt gctcctaaag gattagaagt aggtcaaatc
     2581 gttgaaagtg gtgctgaagc tgacatcaaa gttggtaacg cattaccatt acaaaacatt
     2641 ccagttggta cagtagtaca caacatcgag cttaaacctg gtaaaggtgg acaaatcgct
     2701 cgttcagctg gtgcaagtgc tcaagtactt ggtaaagaag gtaaatacgt attaatcaga
     2761 ttaagatctg gtgaagttcg tatgatctta tctacttgcc gtgctacaat cggtcaagtt
     2821 ggtaacctac aacacgaatt agttaacgtt ggtaaagccg gacgttcaag atggaaaggt
     2881 atccgtccaa cagttcgtgg ttctgtaatg aaccctaacg atcacccaca cggtggtggt
     2941 gaaggtcgtg ctcctatcgg tagaccatct ccaatgtcac catggggtaa acctacgctt
     3001 ggtaagaaaa ctcgtcgtgg taaaaaatca tcagacaaac ttatcgttcg tggacgtaag
     3061 aaaaaataat ataatcaact tatttgggtg tgcggcttaa agctgcacgc acataataag
     3121 aagggaggcg cccaaatggc tcgtagtatt aaaaaaggac ctttcgtcga tgagcattta
     3181 atgaaaaaag ttgaagctca agaaggaagc gaaaagaaac aagtaatcaa aacatggtca
     3241 cgtcgttcta caattttccc taatttcatc ggacatactt ttgcagtata cgacggacgt
     3301 aaacacgtac ctgtatatgt aactgaagat atggtaggtc ataaattagg tgagtttgct
     3361 cctactcgta cattcaaagg acacgttgca gacgacaaga aaacaagaag ataatatcta
     3421 ttaagtagag gaggacatcc taatggaagc aaaagcggtt gctagaacaa taagaatcgc
     3481 acctcgtaaa gtaagactag ttcttgactt aatcagaggt aaaaatgctg ctgaagctat
     3541 tgcaatttta aaattaacaa acaaagcttc atcaccagta attgaaaaag tattaatgtc
     3601 cgctttagct aatgctgaac ataactatga catgaacaca gatgaattag tagttaaaga
     3661 agcatatgct aacgaaggac caacattaaa acgtttccgt ccacgtgcgc aaggtcgtgc
     3721 aagtgcgatt aacaaacgta caagccacat tacaatcgtc gtaagtgacg gtaaagaaga
     3781 agctaaagaa gcttaattaa cttttaagga gggaatactg tgggtcaaaa aattaatcca
     3841 atcggacttc gtgttggtat tatccgtgat tgggaagcta aatggtatgc tgaaaaagac
     3901 ttcgcttcac ttttacacga agatttaaaa atccgtaaat ttattgataa tgaattaaaa
     3961 gaagcatcag tttctcacgt agagattgaa cgtgctgcaa accgtatcaa cattgcaatt
     4021 catactggta aacctggtat ggtaattggt aaaggcggtt cagaaatcga aaaattacgc
     4081 aacaaattaa atgcgttaac tgataaaaaa gtacacatca acgtaattga aatcaaaaaa
     4141 gttgatcttg acgctcgttt agtagctgaa aacatcgcac gtcaattaga aaaccgtgct
     4201 tcattccgtc gtgtacaaaa acaagcaatc actagagcta tgaaacttgg tgctaaaggt
     4261 atcaaaactc aagtatctgg tcgtttaggc ggagctgaca tcgctcgtgc tgaacaatat
     4321 tcagaaggaa ctgttccact tcatacgtta cgtgctgaca tcgattatgc acacgctgaa
     4381 gctgacacta cttacggtaa attaggcgtt aaagtatgga tctatcgtgg agaagttctt
     4441 cctactaaga acactagtgg aggaggaaaa taataatgtt actaccaaaa cgtgtaaaat
     4501 atcgtcgtca acatcgtcct aaaacaactg gtcgttctaa aggcggtaac tacgtaacat
     4561 ttggtgagtt tggtttacaa gctacaacaa cgtcttggat cacatctcgt caaatcgaat
     4621 ctgctcgtat agcaatgaca cgttacatga aacgtggcgg gaaagtttgg attaaaatct
     4681 tcccacatac accatatact aaaaaacctt tagaagtacg tatgggtgct ggtaaaggtg
     4741 cggttgaagg ctggatcgca gttgttaaac caggtagaat tttattcgaa gttgctggcg
     4801 tttctgaaga agttgcgcgt gaagcactac gtttagcaag tcacaaactt ccagtaaaaa
     4861 ctaagtttgt aaaacgtgag gaattgggtg gtgaaacaaa tgaaagctaa ggaaattaga
     4921 gacttaacca cttcagaaat cgaagaacaa atcaaatctt caaaagaaga gctttttaac
     4981 ctacgctttc agttagctac aggtcaatta gaagaaactg cacgtattcg tacagtaaga
     5041 aaaacgattg cacgtctaaa aactgttgct cgtgaaagag aaattgaaca aagtaaggct
     5101 aatcaataat taagtaagag gaggttacaa aagtgagcga aagaaacgat cgtaaagttt
     5161 atgtaggtaa agttgtttca gacaaaatgg acaagactat tacagtactt gttgaaactt
     5221 acaaaacaca caaattatac ggtaaacgag taaaatactc taaaaaatac aaaactcatg
     5281 atgaaaacaa ttcagctaaa ttaggagaca ttgttaaaat tcaagaaact cgtcctttat
     5341 cagcaacaaa acgttttcgt ttagtagaga ttgttgaaga gtcagtaatt atttaataca
     5401 agtttagaga taagggaggt ttaactaatg atccaacaag aaacacgctt gaaagtagca
     5461 gacaactctg gtgctcgtga agttcttaca atcaaagtat taggtggatc tggtcgtaaa
     5521 acagcaaaca tcggcgatgt tatcgtatgt actgttaaaa atgcaacacc aggtggcgtt
     5581 gttaaaaaag gtgacgttgt caaagctgta atcgtacgta ctaagtcagg tgttcgtcgt
     5641 aatgacggtt catacatcaa atttgatgaa aatgcatgtg ttatcatccg tgatgacaaa
     5701 ggcccacgtg gtactcgtat cttcggacct gttgctcgtg aattacgtga aggtaacttc
     5761 atgaaaatcg tatcattagc accagaagta ctttaattaa taacaaccaa tacaaataag
     5821 gaggtgccca catgcatatc aaaaaaggtg acaacgttaa agttatcgca ggtaaagaca
     5881 aaggtaaaga aggtaaagta attgctactc tacctaaaaa agaccgtgtc gttgtggaag
     5941 gtgttaacat tatgaaaaaa caccaaaaac caactcaatt aaatcctgaa ggtggaatct
     6001 tagaaacaga ggcagcaatc catgtttcta atgtacaatt attggaccct aaaacaaacg
     6061 aaccaactcg tgtaggttac aaatttgttg atggtaaaaa agttcgtatc gctaaaaaat
     6121 ctggcgaaga aattaaatct aataattaat aacaagttga aaggaggatc ccactttgaa
     6181 ccgtttaaaa gaaaagttta acactgaagt tactgaaaac ttaatgaaaa aattcaatta
     6241 tagttcagta atggaagtac caaaaataga taaaatcgtt gtgaacatgg gtgtaggtga
     6301 cgcagtacaa aattctaaag tattagacaa tgctgttgaa gaattagaat tgatcactgg
     6361 tcaaaaacca ttagtaacta aagctaaaaa atcaatcgcg actttccgtt tacgtgaagg
     6421 tatgccaatc ggtgcgaaag taacacttcg cggtgaaaga atgtatgaat tcttagacaa
     6481 attaatttca gtatcattac cacgtgtacg tgacttccaa ggtgtttcta aaaaagcatt
     6541 tgacggacgc ggtaactaca ctttaggtgt taaagaacaa ttaattttcc cagaaatcga
     6601 ctatgataaa gtaagtaaag ttagaggaat ggatattgtt atcgtaacga ctgctaacac
     6661 tgatgaagaa gctcgtgaat tgttagctaa cttcggtatg ccattccgta aataattatt
     6721 taaaggaggc taattaagtg gctaaaactt caatggttgc taagcaacaa aaaaaacaaa
     6781 aatatgcagt tcgtgaatac actcgttgtg aacgttgtgg ccgtccacat tctgtatatc
     6841 gtaaatttaa attatgccgt atttgtttcc gtgaattagc ttacaaaggc caaatccctg
     6901 gcgttcgtaa agctagctgg taataaaaaa gagtctgaaa ggaggcaaca atcaatgaca
     6961 atgacagatc caatcgcaga tatgcttact cgtgtaagaa acgcaaacat ggtgcgtcac
     7021 gagaagttag aattacctgc atcaaatatt aaaaaagaaa ttgctgaaat cttaaagagt
     7081 gaaggtttca ttaaaaatgt tgaatacgta gaagatgata aacaaggtgt acttcgttta
     7141 ttcttaaaat atggtcaaaa cgatgagcgt gttatcacag gattaaaacg tatttcaaaa
     7201 ccaggtttac gtgtttatgc aaaagctagc gaaatgccta aagtattaaa tggtttaggt
     7261 attgcattag tatcaacttc tgaaggtgta atcactgaca aagaagcaag aaaacgtaat
     7321 gttggtggag aaattatcgc atacgtttgg taataaaaaa taaggaggtg ccataacatg
     7381 agtcgtgttg gtaagaaaat tattgacatc cctagtgacg taacagtaac ttttgatgga
     7441 aatcatgtaa ctgttaaagg tcctaaaggt gaattatcaa gaactttaaa tgaaagaatg
     7501 acattcaaac aagaagaaaa cacaattgaa gttgtaagac catctgattc taaagaagat
     7561 agaacaaacc atggtacaac tcgtgcttta ttaaacaata tggtacaagg tgtttctcaa
     7621 ggatacgtaa aagtacttga acttgttggt gtaggttacc gtgctcaaat gcaaggtaaa
     7681 gacttaatcc ttaacgttgg ttattctcac ccagtagaaa ttaaagctga agaaaacatt
     7741 actttctcag ttgagaaaaa cacagtcgtt aaagttgaag gtatttcaaa agaacaagtt
     7801 ggagcattag catctaacat ccgttcagta agacctccag agccttacaa aggtaaaggt
     7861 attcgttacc aaggtgaata cgttcgccgt aaagaaggta aaactggtaa ataatagata
     7921 aactctaaag aaaggagtat tgaaatgatc agtaaaattg ataaaaataa agtgcgttta
     7981 aaaagacatg ctcgtgttcg tactaactta tcaggtacag ctgaaaagcc acgtttaaac
     8041 gtatatcgtt caaacaagca tatctacgct caaattattg atgataataa aggcgtaaca
     8101 ttagctcaag cttcttcaaa agacagcgac attgctacta cagcaactaa agttgaatta
     8161 gcaactaaag tcggtgaagc aattgctaaa aaagctgctg acaaaggcat taaagaaatc
     8221 gtatttgacc gtggaggata tttatatcac ggacgtgtta aagcattagc tgaagcagca
     8281 agagaaagcg gattagaatt ttaatttaaa ggagggacaa atacatggct cgtagagaag
     8341 aagagacgaa agaatttgaa gaacgcgttg ttacaatcaa ccgtgtagca aaagttgtaa
     8401 aaggtggtcg tcgtttccgt ttcactgcat tagttgtagt tggagacaaa aatggtcgtg
     8461 taggtttcgg tactggtaaa gctcaagagg taccagaagc aatcaaaaaa gctgttgaag
     8521 cagctaaaaa agatttagta gttgttccac gtgttgaagg tacaactcca cacacaatta
     8581 ctggccgtta cggttcagga agcgtattta tgaaaccggc tgcacctggt acaggagtta
     8641 tcgctggtgg tcctgttcgt gccgtacttg aattagcagg tatcactgat atcttaagta
     8701 aatcattagg atcaaacaca ccaatcaaca tggttcgtgc tacaatcgat ggtttacaaa
     8761 accttaaaaa tgctgaagat gttgcgaaat tacgtggcaa aacagtagaa gaattataca
     8821 attaaggagg gaaaactagt tatggctaaa ttacaaatta ccctcactcg tagtgttatt
     8881 ggtcgtcctg aaacacaacg taaaactgtt gaagctttag gtcttaaaaa gactaacagt
     8941 tcagtagttg ttgaagataa ccctgctatt cgtgggcaaa tcaacaaagt taagcactta
     9001 gtaacagtag aagaaaaata atcaaggagg tgccgaaatg aaattacatg agttaaaacc
     9061 ggcagaaggt tcacgtaaag aacgcaatcg tgttggacgt ggtgttgcga caggtaacgg
     9121 taaaacaagt ggtcgcggac acaaaggtca aaaagctcgt tcaggcggtg gtgtaagacc
     9181 aggatttgaa ggtggtcaat taccattatt ccgtcgttta ccaaaacgtg gttttactaa
     9241 cataaatcgt aaagaatatg ctattgttaa cttagaccaa cttaataaat ttgaagatgg
     9301 tactgaagta actccagctt tattagtaga atctggtgtt gttaagaatg aaaaatctgg
     9361 tatcaaaata ctaggtaatg gttcacttga taagaaattg acagtgaaag ctcataaatt
     9421 ctcagcttca gcagcagaag ctattgatgc taaaggtgga gcacacgagg tgatctaatg
     9481 attcaaaccc ttgtgaactt ctttagaaca aaagaggttc gtaacaagat tttcttcaca
     9541 ctagcaatgt tagtaatttt taaaataggg acttatatac cagctccagg agtaaatcct
     9601 gcagcttttg ataatcccca aggttctcaa ggtgccactg agttattaaa tacttttggt
     9661 ggcggagcct tgaaacgatt ttctattttt gcaatgggta ttgtacccta catcactgca
     9721 tcaatcgtaa tgcaattatt acaaatggat attgtcccta aattctcaga atgggcaaaa
     9781 caaggtgaag taggtagaag aaagttaaat aacgttactc gttatttagc aatttcttta
     9841 gcatttatcc aatctatagg tatggcattc caatttaata attatctcaa aggtgcgctg
     9901 attatcaatc agtcaattat gagttattta ttaatagcac tagttttgac agcaggaact
     9961 gctttcttaa tatggcttgg tgatcaaatc actcagttcg gtgttggtaa tggtatttct
    10021 attatcatat ttgcgggtat tttatcaacg ttgccagcca gtctaattca atttggacaa
    10081 actgcgttcg ttgggcaaga agatacatca ttagcatggt tgaaagtatt aggactttta
    10141 gtgtcactga tactattaac tgtaggtgct atttatgtac ttgaagccgt tcgtaaaatt
    10201 ccgattcaat atgctaagaa acaaactgct caaagattag gttcacaggc aacttatcta
    10261 cctttaaaag tgaactcagc cggtgttatt cctgtaatct ttgcaatggc gttcttctta
    10321 ctgcctagaa cattaacgtt gttctatcca gataaagaat gggcacaaaa cattgcgaat
    10381 gctgcaaatc cttcaagtaa tgttggtatg gttgtatata ttgtactaat tatactattc
    10441 acatacttct atgcatttgt acaagttaat ccggaaaaaa tggctgataa ccttaagaaa
    10501 caaggtagct atgttccagg tattagacct ggtgaacaaa ccaaaaaata tattactaaa
    10561 gttttatatc gtttaacttt tgtaggttct atcttcttag ccgttatttc aattctaccg
    10621 atattagcaa ctaagtttat gggattacca caatcaattc agattggtgg tacaagttta
    10681 cttatcgtta ttggtgtagc gattgaaaca atgaaatctt tagaagctca agtatctcaa
    10741 aaagaatata aaggctttgg tggtagataa ttttaggagg gcacttatga atatcatttt
    10801 gatgggttta cctggcgcag gtaaaggaac tcaagcaagt gaaattgtca agaaattccc
    10861 aataccccac atttcaactg gtgacatgtt cagaaaagct ataaaagaag aaactgaatt
    10921 aggtaaagaa gctaagtctt atatggaccg tggcgaatta gttcctgatg aagtgactgt
    10981 aggtatcgtt aaggaaagaa tttctgaaga cgatgcaaaa aaaggctttt tattagatgg
    11041 cttcccaaga acaatcgagc aagctgaggc attaaataat attatgtctg agcttgacag
    11101 aaacattgat gctgtcatca atatcgaagt tccggaagaa gaattaatga accgtcttac
    11161 aggtcgtcga atctgtgagt catgtggtac aacgtatcat cttgtattta atcctccgaa
    11221 ggtcgaaggt atttgtgata tcgatggtgg taaattgtat caacgagaag atgataatcc
    11281 tgaaacggta gctaatcgtt tgagtgttaa tattaaacaa tctaagccta ttttagattt
    11341 ctatgatcaa aaaggtgtat tgaaaaatat tgatggttca aaagatatta gcgacgttac
    11401 caaagatgtc attgatattt tagatcattt gtaatagatc aacttttagc ggtctatttt
    11461 aagtgaggca tgatttaatc atattagata gatgagagtg gcaattaacg ataattaatt
    11521 cccgtaattt ttgtttaact tataaaatta atcactgata gattcgtaac aataaagttg
    11581 tctattgacg attaccttac tattgcaaaa agggggaagt taatcaatgg ctaaacaaga
    11641 tgtaattgaa ttagaaggta ctgtattaga tactttaccg aacgcaatgt ttaaagtaga
    11701 attagaaaat ggtcatgaga ttttagctca cgtaagtggt aaaatcagaa tgaattacat
    11761 tcgtattcta cctggcgaca aagtaactgt tgagatgtct ccgtacgatt taacacgcgg
    11821 aagaattact tatcgttata aataatcgcc actccataat atagggaggt ataaaaatga
    11881 aagtaagacc atcagtaaaa cctatttgcg aaaaatgtaa agtcattaaa cgtaaaggta
    11941 aagtaatggt aatttgtgaa aatccaaaac acaaacaaag acaaggttaa taaaagagag
    12001 gtgtaaatta atatggcacg tattgcagga gtagatattc cacgtgaaaa acgcgtagtt
    12061 atctcattaa cttatatata cggtatcggt acgtcaactg ctcaaaaaat tcttgaagaa
    12121 gctaacgtat cagctgatac tcgtgtgaaa gatttaactg atgacgaatt aggtcgcatc
    12181 cgtgaagttg tagacggtta taaagtcgaa ggtgacttac gtcgtgaaac taacttaaat
    12241 atcaaacgtt taatggaaat ttcatcatac cgtggtatcc gtcaccgtcg tggtttacca
    12301 gttcgtggtc aaaaaacgaa aaacaacgcg cgtactcgta aaggaccagt taaaacggta
    12361 gctaacaaga aaaaataata ggtaaaggag gcaaatttta aatggcacgt aaacaagtat
    12421 ctcgtaaacg tagagtgaaa aagaatattg aaaatggtgt agcacacatc cgttcaacat
    12481 tcaacaacac tattgtaact atcactgatg agttcggtaa tgctttatca tggtcatcag
    12541 ctggtgcatt aggattcaaa ggatctaaaa aatcaacacc atttgcagca caaatggctt
    12601 ctgaaactgc atctaaatca gctatggagc atggtttaaa aacagttgaa gtaacagtta
    12661 aaggacctgg tccaggtcgt gaatcagcta ttcgtgcatt acaatctgca ggtttagaag
    12721 taactgcgat cagagacgtt actccagtac ctcataacgg ttgtcgtcca ccaaaacgtc
    12781 gtcgtgtata atttatgatg gtattgttac aggtcactga gcaaacattt taaattaagt
    12841 cgacgtatat aaggaggata tttaaatgat agaaatcgaa aaacctagaa ttgagacaat
    12901 tgaaattagt gaagatgcta aattcggtaa gttcgttgtt gaaccactag aacgtggcta
    12961 cggtactaca ctaggaaact ccttacgtcg tatcctacta tcttcattac caggtgcagc
    13021 cgttaagtat attgaaattg agggagtttt acatgaattc tcagcagtag acaatgtagt
    13081 tgaagatgtt tctacaatta ttatgaacat taaacaatta gcattgaaaa tttactctga
    13141 agaagataaa actttagaaa ttgatgtacg tgatgaaggc gaagtaacag caagcgacat
    13201 tacacatgat agtgatgttg aaattttaaa cccagagctt aaaattgcaa cagtatctaa
    13261 aggtggtcac ttaaaaattc gtctagttgc taacaagggt agaggttacg cattagcaga
    13321 acaaaataat actagtgatt taccaattgg tgtaatccct gttgattcat tgtattcacc
    13381 tgttgaacgt gtgaactata ctgttgaaaa tacacgtgta ggtcaaagca gtgattttga
    13441 taaattaaca ttggatgttt ggactaatgg ttcaatcaca ccacaagaat cagtttcatt
    13501 agcagcaaaa ataatgactg aacacttgaa tatcttcgtt ggtcttactg atgaagcgca
    13561 aaacgctgaa atcatgattg aaaaagaaga agatcaaaaa gaaaaagtat tagaaatgtc
    13621 tattgaagaa ttagacttat ctgtacgttc atataactgc ttaaaacgcg caggaatcaa
    13681 ttctgttcaa gagttagctg acaaatctga agctgacatg atgaaagtgc gtaatttagg
    13741 tcgtaaatct ttagaagaag ttaaatacaa attagaagat ttaggattag gattaagaaa
    13801 agaagattga taaaggaggt taactcatgg gttacagaaa attaggtcgt acttctgatc
    13861 aacgtaaagc tatgttacgt gacttagcta catcacttat tattagtgaa cgtattgaaa
    13921 ctacagaagc tcgtgcaaaa gaagttcgca gtgttgttga gaaattaatc actttaggta
    13981 aaaaaggaga tttagcttct cgtcgtaatg cagctaaaac tttacgtaat gttgaaatct
    14041 taaacgaaga tgaaactaca caaactgcac ttcaaaaatt atttggtgaa atcgcagagc
    14101 gttacacaga acgtcaaggt ggttacactc gtatccttaa acaaggccct cgtcgcggtg
    14161 acggtgctga atcagtaatt atcgaattag tataagaatt tatataaata cactaactac
    14221 ttttatataa agcaaatgag tgcaacgata atgtttgcca catacaaata ttgtctagct
    14281 cagagtaccc catcaactaa ataaagattt aaagcgtgaa ctcaacaaac ttatgatggg
    14341 gtgcgcgctt ttttattgaa taagatatcg gacttatgaa cagaaatgtt tttaagtccg
    14401 atttctttat atctactttt ttgagtgcga atctaaaaat atgatcaagc tagtttgatt
    14461 aatttcacta gaactgtatg aagtgaaagt attatcgttg aatcaaacgc ggccaaccct
    14521 atgttagtaa atcatttatt ttattaacgt ttatacataa ttgttagaga gggataaatg
    14581 tccttatcat tagttgttaa acaacacttt aaagactaga aatgagtcag aaaatactag
    14641 taatatatag agaacccagt caatgtcata tacagcgatt tataaatatt gtaaaatatg
    14701 atagatacac gaatcgtaaa ggagagagac aagtggagga taagaattca gttattgtat
    14761 ttaaaaatgt ttcatttcaa tatcaaagtg atgcatcctt cacattgaaa gatgtttctt
    14821 ttagtatacc taaaggtcag tggacatcta ttgttggtca taacggttct ggaaaatcta
    14881 caattgccaa gttaatgatt ggcatagaga aagttaaatc tggagaaatt ttttataata
    14941 atcaaactat aacagatgat aattttgaaa agttaagaaa agacatagga atagtattcc
    15001 agaatccgga taatcaattt gttggttcaa ttgtaaaata cgatgtggca tttggactcg
    15061 aaaatcatgc ggttccacat gacgaaatgc atagaagagt cggcgaagca cttaaacaag
    15121 ttgatatgtt agaacgtgca gattatgaac ctaatgcatt atcgggggga cagaagcagc
    15181 gtgtggctat agcaagtgta ttagcactta acccctctgt cattatatta gatgaggcga
    15241 cttctatgtt agatcctgat gcacgtcaaa atttattgga tttagtgaga aaagttaaat
    15301 cagaacataa tattacaatc atttctatta cgcatgattt atctgaggcg atggaagcag
    15361 atcatgttat cgttatgaat aaagggactg tctataaaga aggcacagcg attgaaattt
    15421 tcgaccatgc agaagagtta acaacaatag gtctagattt gccattccca atcaaaataa
    15481 atcaaatgct gggataccaa acatcattct taacttatga agggctggtg gatcaactat
    15541 gactatacgc tttgacaatg taagttatac ctatcaaaaa gggacaccat accagcatca
    15601 agctattcat gatgttaata cagaatttga acaaggtaaa tattacgcca tcgttggaca
    15661 aacgggtagt ggtaaatcaa cattgataca aaatattaat gcactgttaa agccaactac
    15721 tgggacagtt acagttgatg acattactat tacacataag accaaagata aatatattag
    15781 acctgtaaga aaaagaattg gaatggtatt tcaatttccc gaatctcaat tatttgagga
    15841 cacagtagag cgtgaaatga tatttggacc taaaaacttt aaaatgaatt tagatgaagc
    15901 caaaaactat gcccatcgtc tgttgatgga tttaggcttt tcaagagatg taatgtcaca
    15961 atcaccattt caaatgtcag gtggacaaat gcgtaaaata gcgattgtat cgatattggc
    16021 aatgaatcct gatattatcg tggttgatga acccacagca ggacttgatc cacaaagtaa
    16081 acgacaagta atgcgattac taaagtcatt acaaacagat gaaaataagg caattatcct
    16141 aatttcacat gatatgaatg aagtcgcgcg ttatgcagat gaagtcatcg ttatgaaaga
    16201 aggtagtata gtgtcgcaaa catcaccaaa agagctcttc aaagataaaa aaaaattggc
    16261 ggattggcat attggtttgc cagaaattgt tcagttacaa tatgactttg aacaaaaata
    16321 tcaaacaaaa ttaaaagata ttgccttaac tgaagaagca tttgtaagct tgtataagga
    16381 gtggcaacat gaaaaataaa ttgattatag ggcgatattt accaattaat tcttttgttc
    16441 atcatcttga tccaagagca aagttaatgt ttgtttttct ttttattata ttaatatttt
    16501 tctgtcattc accattaaca tatctttggg tgtttgcact tatcttattc attatgagat
    16561 tagcaaaaat tcaattatgg ttcctaatca aaggtttaac accaatattt ttctttttaa
    16621 tcttcacatt aatgatgcat atatttttaa ctaaaggtgg atatgtatta gttgaatggc
    16681 atggcatcac gattgaaact aatggtattt tagaagggct ttatatatca ttgcgtttaa
    16741 ttgggattgt gatgattgca acaattatga cactatctac aagtccaatt gatttaacag
    16801 atgcgtttga aagattactt gcaccactaa agatgtttaa attaccagtt catcaattaa
    16861 gtatgataat gtccattgcg ttacgattca tcccgacgtt aatggatgag ttagataaaa
    16921 ttattttggc acaaaagtcg cgtggttctg aaataagttc agggaacatt gcaacacgta
    16981 ttaaatcatt tattccacta ctagtgccac tattcatctc agctttccaa cgcgccgaag
    17041 aattagcggt cgcaatggaa gttaggggtt atgatgccaa tgttaagaga acgagctaca
    17101 gacagcttaa atggcaattg agagatacgt tatctttaat catgattatc ccaattgcta
    17161 ttattttatt cgtattaaaa tattcaggag tgtaaattca tgcgtatatt agtagaaatt
    17221 gcgtatcaag gaaataattt tctaggtttt caaattcaac aaaatggtcg aacggtacag
    17281 caacaatttg aaaagctatt acaacgcatg cataaaagac atgtgagaat ccatccttca
    17341 agtaggactg atagaggtgt acatgcgata cagcaatact ttcatttcga cacagagctg
    17401 aatataccta tgtcacaatg gcaatatgca atgaaccgaa cgttacctga tgatatttat
    17461 gtcaacaatg tagttacagt cgatgacgat tttcattgtc gttacgattg tgtaggtaaa
    17521 cgctatcgat ataaagtata tcaagcacaa caccgcgatc catttcaaag tggtttgaaa
    17581 acatttattc ctgaaacgtt ggatttagat aaaatgaaca gagcggcaca acaattcata
    17641 ggtacacatg attttacggg attttgttcg caaaaaactg aagtagaaag caaggttaga
    17701 acattatacc aaagtgaaat tgtaaagaca gatgatggct ttgattatat tgtcactgga
    17761 tctggatttt tatataacat ggtgcgtgtc ttagttgcat ttttaataga agttggaaaa
    17821 ggacgacatg aaatttctga tgtgccgaag ctacttgaat caaagaatcg taagaatgtt
    17881 cctttcactg caacggctga aggattatat ttagagaaga tttatttaga tgaaaatgag
    17941 ttattaaaag actttggcaa cgatatcaaa atacatcgta aaaaatcatt acaaaatgac
    18001 tgattgccat tgacaaaaag cttgtgaaat tataagatta ttaacggtat tgttttatat
    18061 ccaccccacg ataagccccg gaaacttatt gtgttacaag atatataagc agaaacgaac
    18121 aacagttaac aaaataaatg aaattaaacg ttttaaaaat gaaacaaatg aaatcatcta
    18181 ttaggttatg aaactgttta tagcttgaat agaagcattt attttttagg aggacaatta
    18241 ttatgcgtca aacatttatg gcaaatgaat caaacattga gcgcaaatgg tatgttatcg
    18301 atgctgaagg ccaaacatta ggtcgtttat catcagaagt agcatctatc ttacgcggta
    18361 aaaataaagt aacttacaca ccacacgttg atactggtga ttatgtaatc gttattaatg
    18421 catcaaaaat cgaatttact ggtaacaaag aaactgacaa agtttactac cgtcactcaa
    18481 atcacccagg tggtatcaaa tcaatcactg ctggtgaatt aagaagaact aacccagaac
    18541 gtttaattga aaactcaatt aaaggtatgt taccaagcac tcgtttaggc gaaaaacaag
    18601 gtaaaaaatt atttgtatat ggtggcgctg aacatccaca cgctgcacaa caaccagaaa
    18661 actacgaatt acgtggttaa ttagaaggag gaaatgactt tggcacaagt tgaatataga
    18721 ggcacaggcc gtcgtaaaaa ctcagtagca cgtgtacgtt tagtaccagg tgaaggtaac
    18781 atcacagtta ataaccgtga cgtacgcgaa tacttaccat tcgaatcatt aattttagac
    18841 ttaaaccaac catttgatgt aactgaaact aaaggtaact atgatgtttt agttaacgtt
    18901 catggtggtg gtttcactgg acaagctcaa gctatccgtc acggaatcgc tcgtgcatta
    18961 ttagaagcag atcctgaata cagaggttct ttaaaacgcg ctggattact tactcgtgac
    19021 ccacgtatga aagaacgtaa aaaaccaggt cttaaagcag ctcgtcgttc acctcaattc
    19081 tcaaaacgtt aattgtcgga cgatatatac aaaacacctc gaagttatgt cgaggtgttt
    19141 ttttacgttt gtgtggggaa tatggaacgt gtagagtata atgaattttc ctttatttaa
    19201 tgtctcagga atagaataaa ttcaactatg tattagtgat ttgcacgtat gatttgtaca
    19261 tgcttaaaca atcaaaagtg tccattatta aattatcatt tagcttgcgg aggccgtatg
    19321 ataaccggac gattgtgttc taatcataga ggctcaatgg aagaactacg ggacgaaaac
    19381 cagacgataa ctcaagaacc atgatttaag agtattatca aaataaggag tgacttaatt
    19441 gaaagattct atattttgga agaaagcttt tattcctgtt tattttattg ttgcgatgct
    19501 ggtgtttcta ctttttaggt tttatattaa aacagacaac ttttctatgt atttaatgat
    19561 tatcttctta atttgtttag gaactgcatc tatcatttat aactataaaa acaatcgata
    19621 aatataagta tgaaatgtat aagtcacagt tagatctaag tcttgctgtg gcttttttta
    19681 tcgaaatgac aattttaaat cgccaaaaac aacattttca gtccgccata aaacaacaat
    19741 ttcaaatcgc cattgactaa acacgacatc caaaaattat tgtaacaatt cttcacatca
    19801 atcaatcctc catcactcat aatcactccc tacaaaatct actcatgcat ttttggaata
    19861 cttagtatta caaataacga tttttattca tcttacaaag gttatataat gtaatgaagg
    19921 caatttttat gtattgcaaa tctaatcgta catgtaaaat tttgataaac atcattaatt
    19981 ttgcgtaact atcattagat tacaaatcac aatgtaatta catgtaatac acatctatac
    20041 atcacatttg aagggaaatg aatataaatg actgataaaa agtacactgc agccgatatg
    20101 gttattgata ctttgaaaaa taatggggta gaatatgttt ttggtattcc gggtgcaaag
    20161 attgactatc tatttaatgc tttaattgat gatggtcctg aacttattgt cacgcgtcat
    20221 gaacaaaacg ccgcaatgat ggcgcaaggt attggaagat taacaggcaa accgggtgta
    20281 gtacttgtta caagtgggcc tggtgtaagt aatttaacga ctggactatt aacagctaca
    20341 tctgaagggg atcctgtctt agcgttaggc ggccaagtga aacgtaatga tttattacga
    20401 ttaacgcatc aaagtattga taatgctgct ttattaaaat attcatcaaa atatagtgaa
    20461 gaagtacaag atcctgaatc attatcagaa gttatgacaa atgcaattcg aattgctact
    20521 tcagggaaaa atggcgcaag ttttattagt attccgcaag atgtgatttc ttcaccagtt
    20581 gaatctaagg cgatatcact ttgtcaaaaa ccaaatttag gtgtaccaag tgaacaggat
    20641 attaatgatg tcattgaagc gattaaaaat gcatcatttc cggttttact agctggaatg
    20701 agaagttcaa gtgcagacga aacgaatgcc attcgcaaat tagtagagcg taccaattta
    20761 cccgttgtag aaacattcca aggtgcgggt gtgattagtc gtgaattaga aaatcatttc
    20821 tttggccgag tgggtttatt ccgtaatcaa gttggtgatg aattattacg taaaagtgat
    20881 ttagttgtta caatcggtta tgatcctata gaatatgagg ctagtaactg gaataaagaa
    20941 ttagatacgc aaataatcaa tattgacgaa gttcaagctg aaattactaa ttatatgcaa
    21001 ccgaaaaaag agttgattgg taatattgct aaaacgattg aaatgatttc tgacaaagta
    21061 gatgaaccat ttataaatca acaacatttg gatgaattag aacagttaag agcgcatatt
    21121 gatgaagaaa ctggtattaa agcgacgcat gaagaaggca tcttacaccc agttgaaatt
    21181 atagaatcta tgcaaaaggt attaactgat gaaacaacag tgacagttga tgttggtagt
    21241 cactatattt ggatggcacg taatttcaga agttacaatc caagacattt attatttagt
    21301 aacggtatgc aaacgcttgg tgtagcatta ccgtgggcaa tttcagctgc acttgtacgc
    21361 cctaatacgc aagttgtatc cgttgctggc gatggaggct ttttattttc atcacaagat
    21421 ttagaaacgg ccgtacgtaa aaatctaaac attatccaac ttatttggaa tgatggaaaa
    21481 tataatatgg ttgaattcca agaagaaatg aaatataaac gttcatcagg cgtagacttc
    21541 ggtcctgtag atttcgtaaa atatgctgaa tcatttggcg caaaaggttt acgagttact
    21601 aatcaagaag aattagaagc ggcaattaaa gaggggtatg aaacagatgg cccagtatta
    21661 attgatatac ctgtaaacta caaagataat atcaaacttt caacaaatat gttacctgac
    21721 gtatttaact aaaacaaaga taaatgttaa tgaggagtgg gaaattttat ggctagtgtt
    21781 ttatatcaac atggtacatt aggcacatta atggcaggat tattaaaagg aactgcatca
    21841 ataaatgaat tattgcaaca tggtgaccta ggtatcgcta cactaacagg ttcaaatggt
    21901 gaggtcatct ttttagatgg aaaagcttac catgcaaatg aacataaaga atttgtagaa
    21961 ttaaaaggtg atgagttaac accatatgca actgtaacta aattcgtagc agatacaagc
    22021 tatgaaacga aagataaatc ttctgaagca gtttttgcag aaattaagga aaagatgttg
    22081 agtgaaaatt tattttctgc agtaaaaatt tcaggcttat ttaaaaaaat gcatgtacgt
    22141 atgatgccgg cacaagagcc accttataca cgtttaatcg attcagctag aagacaacct
    22201 gagcaaactg aaacatacgt caaaggttca gtcgttgggt tctttacacc agaactattc
    22261 catggtatcg gatcagcagg attccatgta cactttgcaa atgatgatcg taattttggt
    22321 ggacatgtct tagattttga agtagaagat gttaaggtag aaatccaaaa tatagaaaca
    22381 tttgaacagc attttccaat tcaagatgaa gatttcataa atgcaaatat tgactataaa
    22441 gatattgcgg acgaaattag agaagctgaa taatgaatgc ggaaatgtaa tgacggttta
    22501 tgaaaattga cttcaaaatg cgttatttgg aaatggtagt ttttttatat gattaagcaa
    22561 gactacaata gaacaaatat atttataatt acgtctaagt aataaaataa atcccttcac
    22621 tattagcggt agtgagggga tttatttagg ttccagatat ttgagatttg ctgttatgtt
    22681 tagataataa attgaggaag acattaatat agaatttact ttagtatagg ccaatctaat
    22741 ctattgtcag tagaagcaat caataataaa ttacaagttc gatcattcat ttcatactaa
    22801 aaagacaaac gcaatttttc agcgtttgcc tcatgtaatt attcttcatt ttcaaattat
    22861 tatttaacta caatttgtat ctgtttgatt gtacttggat caattgaatt tttagcgtcg
    22921 tcatttttct ttagattcac tactttttta ttaccatcat tcaaagtaag tgtataagtt
    22981 gcagtttgcg cattattaat tttttctgtt gtaacaccac gttgtgaagc taattcattt
    23041 tttactttac tgtcaatttc ttgataaagt acctttttat tttctggaaa aacaaagtaa
    23101 gttcgatgta atgcagtaat accatctact gagatcgtgt aaggtacagt gtgtttccca
    23161 tccattgtca attccttata accgttatta ctatctgcag atgcttcatg actcggtaaa
    23221 gctgcaccta ctgttgataa taatcccaat gctaaagtgg cagtaacaaa tgattttagt
    23281 ttcataactc aatctccttt ttgttttgta ggatattaca ctatttgtat aacacgtttc
    23341 ataaagttta aaacatactt tttgattttt ttatattttt gatttttagt aatagttttt
    23401 aacgagggat atgactcgtg tattttgaaa aaagaaatat aataaaccat catgttacta
    23461 taattttaaa tcattaatta tcatctttat ttaatgtttg ttgtaattca aagcgcacgc
    23521 cggattcatt agaagtcgac gtatttttgt ttgtaataga gtatccagtc attgaaattt
    23581 tagattcaat atctgaagcg gtatttgtag attcgggatt atagaagcta cattcataag
    23641 ttttatcatc tttcttttta agtataaaca taccttttgc tttaacttcg actttagtgt
    23701 tgttaatgat aaaagtttga gtgctatcgc tgtaattaac accagcccaa accgattcat
    23761 tatctttcac aacgtggaag tcatcatctt gtttaacgac agttacttta tctttttttg
    23821 tattaaagtc atctttagat aagcctggat acaacacata tgcatattta ctatctgtat
    23881 tagaatgctt ttgagttact tcataatatt gattagtttt actgtcttgc ttttgacttt
    23941 tatttatatc actccactta ccagtatgac tttctttttt tacagttatt ttcgattcgt
    24001 ttaaaaaatg atagcctata ttgtttttag gtttattggt tgactctaaa aagacggaat
    24061 tggttccctg attatcggaa gcggttgttt gtttatcgtc tgtatataac gtatacccat
    24121 tcgctttgcg attttcaatc gttgtaactg gattctttga tgaatcagta cttttaatgc
    24181 cagttcctaa gaagacaatt ttatcgttta atatgaaata tgattttttg gcagttaaag
    24241 ttttgtcctg attttcaaaa tccattccga tactagcatg ttggtcatcg aattttgttc
    24301 cgcctacaaa agttttactc gactttttaa catccgtacc tttaggttct tcattttcta
    24361 aagttgttgt acctgctaaa cgtttcatat cagctgtcgc ccagaagtta tcacggtagt
    24421 gtttcacatc gctattgtat aaataagaca ttccagcacc agtgtgccaa ccttttaagt
    24481 tctctccgtt gatactttcg taacgtgcga cgtttttcga cgtcatactt aaaccaaatg
    24541 caaagtctaa gtctttattg tgataggtga cacgatccat gtcattatat attttaagtt
    24601 gttgtgttaa accgttagta gaaatagtgc tgtcttccat taaagacttc attttgctta
    24661 tatccgaata agagcttaat gtatcatttt ggccgtaact tgaatcagat ttaacagaag
    24721 ttttaacgat ttgcttatac ttagcttttg ttgaatcatc catggcatca ctcaatctca
    24781 acaatgattt cattacagtc gctgacgctg tatgactcgt ttcattttca cgactgatag
    24841 ctctacctct tgataaatcc atcatttcac ctttataaat gagtggcata aatccgtcgt
    24901 caatccatga ctttaaggtt gtatcatttt gggttttatc attaaaaggt gtttctttta
    24961 tcatcggcat catttgagaa atcccctcta agagtacaac gccataagca ccagtgtatg
    25021 ggacgtcttg atgatcaata taagagccgt ctttatagaa tccattacgt tctttaccag
    25081 ttgcattact ttgaacgtaa gtgaagactt tattaaatgc atctattgaa tttttagtta
    25141 tatctttatc ttcttcgata atactttcta aaagttttac tttagcaatg tctactaaat
    25201 ttcccccttt agcttgttca ggttgtccta ctgatgataa tattttgtca ctttctgggg
    25261 cgaaagtttt gataggggca gtgtattttt tcttttcgtc atttgaaaat tgatcattca
    25321 gcaatataag ggtatttgtt aatgctctag gtgttccaat ttcataatcc caccaattta
    25381 aattggtatt tctagaagtt ttatttttaa agtttgaggt caaatcagca acttttttat
    25441 ctggttcttt tccatatgca tttttatgca gccactcaag ggcatctttc actttcttct
    25501 tgttttcatc tatttttaac ttagtatctt tatgcttcat cgcttctgcg attttctcaa
    25561 tattacgata ggtacgagtc atatccgcag acttcgtatc taaatctttt gagttttccc
    25621 acaagtattt cctatcactt tcagttttca tctcactaag taatttcttt gcctcattct
    25681 ccgtagcctc aaacttcttc ttcattgcgt cattcttctc atcatactta tcataaccat
    25741 agttaacgtc cagccatgtg ttcctcaact tttcataatc tggcgtttga acattcgtat
    25801 cagccacagc gatttgatat ttatcaatac ttctgaattc accaccattc aaagtaatca
    25861 caccagccat taataacgta atggtggata atttttgcca tttctttatt ctatatgtca
    25921 ttgacatgtc tcctttttgt gttgcgcgtg cgcaatgaat attatgatta aataatgatt
    25981 caatttttca aaattcgtta acgtatacaa atgactgtct actgtcaaac aatccacaaa
    26041 gaatgttgat gcatataaac aatcgatcac ccaaattttc cgcatcaccc aaaaattgca
    26101 acgaaaataa tttttaaaaa aataaatatt atcaatctta gtgattatat cattttaaat
    26161 atttaagtta tacaattttt aaagtaatta aaaacgatga aatgtaaaat gttaaaaaag
    26221 agacaaattt aaatttgagt aataaaataa gaggtattca aacgaggatg gtaatatata
    26281 aagcaacatt acttaagatg ccaccaacat aactgaaagg atagtcgcaa tgaaaactaa
    26341 agaagtcgta gcgctcatga atatatctca agacacttta agatattatg aaaaggttgg
    26401 tgtgattcca cctgttaatc gagatgaaaa tggatataga atatataatg atagtgattt
    26461 aaattggatt tatttagtga aaaatttgcg aaatgcaggc gtcagtattg aatcgcttat
    26521 cgaattttgc aggttagcgc agcttcctaa aaatgaaaat attcaagcac agcaaaagca
    26581 aattttaaat aagcaactcg aagaattaaa tgaaaactta aagacaattc atgatgcgag
    26641 agatttacta caatataaaa ttgataatta tgataatcat attgctaaaa tcaatgctag
    26701 tgataatcat gatgacaatg ttgaacgtct ttgggagaga aagtaaacct atggcttgct
    26761 atagagttcg ctccatggtt tatagtggac ttacaagata aaggaggttc attttatgaa
    26821 tcatattgaa ataagtaaag atgtaaagat accagtatta ggcttcggag tatttcaaat
    26881 tcctcaagaa caaacagcag aagcggttaa agaagcaatt aaagcgggct atagacatat
    26941 tgatacagcg caaagttatt taaatgaaac tgaagtaggt caaggtattg aagcttcagg
    27001 tattgataga agtgaactat tcattacgac taaagtatgg attgaaaatg taaactatga
    27061 agatactatc aaatcaatcg aacgatcatt acaacgatta aatttagact atcttgatct
    27121 agtgcttatt caccaacctt ataatgatgt atatggctca tggcgtgcat tagaagagtt
    27181 aaaagaaaat ggtaaaatta aagcaatcgg tgtatctaac ttcggcgttg atcgaattgt
    27241 agatttaggt attcacaatc aaattcaacc acaagtgaac caaatagaaa ttaatccatt
    27301 ccatcaacaa gaagaacaag ttgcagcact tcaacaagaa aatgttgtgg tagaagcttg
    27361 ggcaccattt gcagaaggta aaaatcaatt attccaaaat caattgttgc aggccattgc
    27421 agataaatat aacaaatcaa ttgcacaagt tattttacgc tggttagtag aacgtgacat
    27481 cgttgtactc gcaaaatcag tgaatccaga acgtatggct caaaacttag atatatttga
    27541 ttttgaactt acagaagaag ataaacaaca aattgcgaca ttagaagaaa gtaatagcca
    27601 atttttctca catgctgatc cagagatgat taaagcgctt acaagcagag aattagatgt
    27661 ttaatcataa tataacaaag taaatcattt acattacgct aacctgaact tggatgttaa
    27721 agtctatgtt tcaggttatt ttattactct taaaagtgaa agg
//
LOCUS       DBNENW010000018        27516 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNENW010000018 DBNENW010000000
VERSION     DBNENW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.018"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            21..920
                     /locus_tag="AC1LW8_000623"
     CDS             21..920
                     /locus_tag="AC1LW8_000623"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772488.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            913..2145
                     /locus_tag="AC1LW8_000624"
     CDS             913..2145
                     /locus_tag="AC1LW8_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772489.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            2129..2314
                     /locus_tag="AC1LW8_000625"
     CDS             2129..2314
                     /locus_tag="AC1LW8_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772490.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            2363..2689
                     /locus_tag="AC1LW8_000626"
     CDS             2363..2689
                     /locus_tag="AC1LW8_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HIP4772491.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            2719..3738
                     /locus_tag="AC1LW8_000627"
     CDS             2719..3738
                     /locus_tag="AC1LW8_000627"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIP4772492.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(3974..4627)
                     /locus_tag="AC1LW8_000628"
     CDS             complement(3974..4627)
                     /locus_tag="AC1LW8_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIP4772493.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPIHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNELYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            4801..5487
                     /locus_tag="AC1LW8_000629"
     CDS             4801..5487
                     /locus_tag="AC1LW8_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIP4772494.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(5553..6812)
                     /gene="sdpC"
                     /locus_tag="AC1LW8_000630"
                     /gene_synonym="lyrA"
     CDS             complement(5553..6812)
                     /gene="sdpC"
                     /locus_tag="AC1LW8_000630"
                     /gene_synonym="lyrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpC"
                     /protein_id="HIP4772495.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            7102..8037
                     /gene="hutG"
                     /locus_tag="AC1LW8_000631"
     CDS             7102..8037
                     /gene="hutG"
                     /locus_tag="AC1LW8_000631"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HIP4772496.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQESTSGTSFRQILEEDDNTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLNH
                     VSPPIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(8530..9414)
                     /locus_tag="AC1LW8_000632"
     CDS             complement(8530..9414)
                     /locus_tag="AC1LW8_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIP4772497.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIKSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            9574..11235
                     /gene="hutU"
                     /locus_tag="AC1LW8_000633"
     CDS             9574..11235
                     /gene="hutU"
                     /locus_tag="AC1LW8_000633"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIP4772498.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            11235..12473
                     /gene="hutI"
                     /locus_tag="AC1LW8_000634"
     CDS             11235..12473
                     /gene="hutI"
                     /locus_tag="AC1LW8_000634"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIP4772499.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            12749..13870
                     /locus_tag="AC1LW8_000635"
     CDS             12749..13870
                     /locus_tag="AC1LW8_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HIP4772500.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            13981..14862
                     /locus_tag="AC1LW8_000636"
     CDS             13981..14862
                     /locus_tag="AC1LW8_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4772501.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            15349..15918
                     /locus_tag="AC1LW8_000637"
     CDS             15349..15918
                     /locus_tag="AC1LW8_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772502.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      15971..16066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            16143..17456
                     /locus_tag="AC1LW8_000638"
     CDS             16143..17456
                     /locus_tag="AC1LW8_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIP4772503.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKSYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            17623..18129
                     /locus_tag="AC1LW8_000639"
     CDS             17623..18129
                     /locus_tag="AC1LW8_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIP4772504.1"
                     /translation="MAKFNVENEHVEVEVEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(18187..18951)
                     /locus_tag="AC1LW8_000640"
     CDS             complement(18187..18951)
                     /locus_tag="AC1LW8_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP4772505.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            19281..20885
                     /locus_tag="AC1LW8_000641"
     CDS             19281..20885
                     /locus_tag="AC1LW8_000641"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HIP4772506.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(20951..21481)
                     /locus_tag="AC1LW8_000642"
     CDS             complement(20951..21481)
                     /locus_tag="AC1LW8_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772507.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            21749..22666
                     /locus_tag="AC1LW8_000643"
     CDS             21749..22666
                     /locus_tag="AC1LW8_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIP4772508.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFISPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            22741..23376
                     /locus_tag="AC1LW8_000644"
     CDS             22741..23376
                     /locus_tag="AC1LW8_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4772509.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            23387..23578
                     /locus_tag="AC1LW8_000645"
     CDS             23387..23578
                     /locus_tag="AC1LW8_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772510.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(23674..23859)
                     /locus_tag="AC1LW8_000646"
     CDS             complement(23674..23859)
                     /locus_tag="AC1LW8_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772511.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIIVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(23856..24212)
                     /locus_tag="AC1LW8_000647"
     CDS             complement(23856..24212)
                     /locus_tag="AC1LW8_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772512.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            24492..25871
                     /locus_tag="AC1LW8_000648"
     CDS             24492..25871
                     /locus_tag="AC1LW8_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP4772513.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     IAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(26258..27130)
                     /locus_tag="AC1LW8_000649"
     CDS             complement(26258..27130)
                     /locus_tag="AC1LW8_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP4772514.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFTQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT         9196 a         3796 c         5367 g         9157 t
ORIGIN      
        1 atttttttag gaggcaaagt atgggacttg taattgaaca tgttacgaaa cgttttggca
       61 agatgacggc agtaaatgat atttcattag agttagaatc aggcaaaatg ttaggttttc
      121 taggaagaaa tggtgcaggt aaaacaacga catttcgtat gattttaggc ttaagtgaac
      181 caactgaagg acacattacg tataacggta aaaaactaga taaaacaatg tacaatcgta
      241 ttggttattt accggaagaa cgcggtttac atgggaaatt gacagttgaa gaagaattga
      301 aatatttggc aactttaaag ggaatgtcaa aaacagaaat acaacagcaa atatcgtatt
      361 ggcttgagcg ttttgatatt actgaaaacc gcaaaaaacg aattgatagt ctatcaaaag
      421 ggaatcaaca aaaaattcag ctgttagcaa gtatgttaca taaaccagaa ttactaatat
      481 tagatgagcc tttcagtggt ctagacccag ttaatgttga gttattaaaa gaagcagtta
      541 aagacttaaa tgattggggc agtacaatcg tttatagttc tcaccgtatg gagcatgttg
      601 aagaattatg tgatgatgtt tgtattttag ataaaggtca acttgttgtt tctggtgata
      661 tcaatcatgt acgtgcatct aatggtaaca agaaagttgt cattgaatca gaaacaacac
      721 taccagattt aacaaacatt agaggcatca ttcatagtga aaacatgaag caaggtcttc
      781 agttgacgat tgagaatgaa gacgtagcca aagatattta tcaagttgtg gcacatcaag
      841 gttatgttaa acgattccaa gttgtagaac catcattaca agatatattc attgagaagg
      901 ttggtggcaa ggatgcataa gttttgggcg acatttactt tgacatatat caaaaaaatt
      961 aaagctaaat catttgtaat ttttatgatt attattgctg ccttaatgat tggcctatct
     1021 aacattgata agattattaa catgtttgat gatggtccag ataaaattgg tgtagcagca
     1081 ccgaatgaac aaatctataa ggttttcaag caacaggcga atacatttca tagtgatgct
     1141 aagtttacta aagtttctat agaagatgct gaaaaagaag taaaaaaaca taaacttgat
     1201 aaagcttata ttatcaaggt gaatcaaaat cgcacgttac aagggacaat cattagtgag
     1261 aagcgagtaa gtcatgaaga ttcacaaaaa gtacaagcac tgcttacagc tattcaaaca
     1321 aatatggtag ccggggaatt aaatatcaac aaagaagact tacaaaaatt acaagcacaa
     1381 agtaaagttg ataataaggt gatttctaat gatgaagtcg ataaagtatc agaaggacaa
     1441 aagattttca actatgcttt agcttatggc attatctttc taatgttctt tattgtttta
     1501 aattatgcat ctcaaatagc tatggaaatt gctagtgaga aaacatcgcg tgtcattgaa
     1561 atgattatta caagtatttc accaatccaa catatttttg ctaaaatttt aggcgttata
     1621 gcagttgccg tgactcagat tattttaatt gtattgatgg cagttatatg catttttgcc
     1681 tttgacttaa aagacttgct acaaggtttt aacgtggaaa tgaatcaatt gtcttggcaa
     1741 attattattg tcggaattat aagtagtatt gttggaatta tggcgtatgt attacttgca
     1801 gccatcctcg gatcattaac atctagaata gaagatttaa atcaatcttt gatgccactg
     1861 acattacttg gtatgattgc attttatatc gcgatattca cgataaataa tcctgatggg
     1921 cgacttgctt taatcacaag ttatattccg tttttagcgc catttcaatt agttgtacgc
     1981 gctcaaactt cagggcttca aatatatgag gtcatcttaa gttctttaat ctcaattgta
     2041 atggtagctg tcttgatatg gattgcaatt aaaacatata aagattcagt acttacattt
     2101 gaacgtggct tatttaattc aatgaaacgt gtatttaaaa aataaaattg atgagagtga
     2161 cacacattta tcatataaaa tgatgatgaa agcgtgttac tcttattttt ttatatttag
     2221 ctatagaata atgtctaaaa aagtgtacaa tatgaatgtg aaaacgaact ttttaaaatg
     2281 ggaatgtgaa gtaagaagaa atgtaaacaa atgatatgta gccgatagat tattggtagc
     2341 aaatgtgtga tggagtgatt tcatgttata tccgattttt atatttatat tagctggctt
     2401 atgtgaaata ggtgggggat acctgatttg gctgtggctt agggaaggac agtcttcact
     2461 tgttgggcta ataggcggtg cgatactcat gttatatggt gtcattgcga catttcaatc
     2521 atttccatca ttcggaagag tatatgcagc atatggtggc gtatttatca tcatgagttt
     2581 gatttttgcg atggtcgtag ataaacaaat gccagataaa tatgatgtta tcggtgcaat
     2641 catttgtatt gtaggtgttt tagtaatgtt gctacccagc agagcttgaa cacttgtggt
     2701 tagaagggag aagtaaaaat gatagttgaa gttgagcacc aaagacaagg tgtagattta
     2761 atcaaaattg ataatgatga aactaaaatt atttttacga attatggagc aagagtagtg
     2821 tcttggaaat atcatgataa taatattgtt cttggaaatg ttgttgaagc ggatgaattt
     2881 tatcatagta atccttttaa atttggtgct tctataggaa gatatagcgg tagaattgat
     2941 aatgctaaat ttaagttgaa aggtaaagaa tatcagttag aaaaaaacaa cggcgaacat
     3001 catttacatg gtggatgtca tggtttggac aataagttat ttgattatga aattagaaat
     3061 gaaattgctc aaattaaagt gatatttaag acggtgttga aatcagctga tgatcatttc
     3121 ccgggtgata tcgatgtgac gattacacat atttatgatg cggatcacca atggtcgatt
     3181 gaatacgaag cagttgcatc agaagacaca ttgtttagtc ctacgaatca tgtgtacttc
     3241 aatttgaatc gagataataa tgtcgttgat aatcatcgca tttcaagtaa tcaattagat
     3301 atgtatgtat tagatgagcg aaatattgtt acaggtgata ttctagattt acatgaagtg
     3361 tttgaagata acaagataaa attgagtgat atttttacat cacaacatgc gcaattaagc
     3421 caacaaatga cacgttttgg tggtctcgat cacccattta cagttggtga acataaaatg
     3481 tatgttgaaa atcatgaatt tatgttagag gtcgatacag atatgcctca tgttgtgttc
     3541 tttactttta accagcctga tgagtgggat agtccattta atatttataa accacattct
     3601 ggctttacat tagaaacaca atttttacca aatgatatta atttatatgg tgaaaaagca
     3661 ccgtcaattt taaaagctgg tgaagtgttc aattcgaaaa caagtttccg tatcgaagaa
     3721 aaagtagaaa atgattaaaa gtatcggcat ttatataata tttatgttat ttggcggaat
     3781 tttgaatagg ttatcaagaa tcgtatcaaa tgaagtgaca tcattaatat acagtttgaa
     3841 ataaagaaaa tatataacat aaaaaaggaa tcaatagcat cagtggttat agataagact
     3901 attatacaaa cgatgtgtaa gttgattcct ttttgttttg aaaagttcat ttaacactta
     3961 taatgaaagg taattatttc aatgttctgt tatacatttt ttgaagttta tcgcgtcttg
     4021 ttggtgacag aaatggattg tttagtgcat atcgtaacat atcttgattt tgtctatcat
     4081 tataataaag ttcattaagc tgttgtacag ataaacgatg ttcgattggt gcttcttgta
     4141 ttaagtaaag ctgatcattt ggagatacat agccttgttt taatacccgg aaatagaaac
     4201 ctgttttacc agatgtagac atgcgcttca ctaaatcagg aatattatat tttgcttgaa
     4261 ttttccagca tggttctcta atttcagaaa cctcaattaa cgcttcacct aaacgatatt
     4321 gattaccaaa gtaaacatca gattcgtcta aataatcaaa tgttaaattc tctccaaaca
     4381 tcgcatgaat aggtagtgta ggtaaatcat cttgatacag tgcataatta tgcttactaa
     4441 acccacaaac tgctttatgt ggtccaccat gatctttata agcctgttca tcaccagtga
     4501 aaccagttga agataaccat gttgtttgtg aaatcttagt tttatctaaa gcagagcgca
     4561 tcggtctttt cgagctataa ggcaaatctt ggatttttcc tgtcgaaatt gcgtgaattg
     4621 atatcatatc attctcctta acgagtatta tttaaaatat attgtactat aaaacatttt
     4681 tagaattgta aattctgatt gatatgttta aaaaaatgtt agaaataagg gtatgttaat
     4741 ttatacgagt catgtaaaat atgaattgaa tagattacat aaatatggga ggctaccttc
     4801 atgaaagatg tcaaagcact caagttaatg acattaaatg atgttttaag tcaaatcaat
     4861 ggtgacatga cgctgggaat tggtacagga agtacaatgg aattactctt acctcaaatg
     4921 gcgcaactaa ttaaagaacg tggttacaat attacaggtg tatgcacttc taataaaatt
     4981 gcatttttag caaaagaatt aggtattaaa atatgtgaaa tcaatgatgt tgatcatatc
     5041 gacttagcaa ttgatggtgc tgatgaagta gatccatctt taaatataat taaaggtggc
     5101 ggtggtgcgc tgttcagaga gaaagttata gatgaaatgg catcgcgttt tgttgtggtt
     5161 gtcgatgaaa cgaaaatagt tcaatactta ggtgagacgt ttaagttacc agtagaggtg
     5221 gataaattta actggtatca catattacgt aaaattgaat catacgctga tataaaagta
     5281 gaacgtcgtg taaacgaaga tgtcgcattt ataactgata atggcaatta tattttagat
     5341 tgtaagttac caaaaggaat tgacccatat aagttccatg agtatttaat tcatctgaca
     5401 ggtgtgtttg agacaggata cttcttagat atggcagatc aagttattgt aggcactcaa
     5461 gagggtgtta aaattttaga aaaataatat atagtataga aaagcgcttg ctagcatcat
     5521 ctttttaaga ggtactagca agcgctttgt tattatttgt ttttatctga agattgttct
     5581 tcagtttttt cgatatgttt ttctgcatca attacaactg atgaacgtct gtcttcttta
     5641 taagtcgcag gttcttttaa tgattgctct atttcatctt catttgaatc gtttgttaaa
     5701 tcgtcacttt gtggtgcttg tttaactgat tctgctgatt ctactgactc cgaagtgatg
     5761 tcatgatctt catcatgatt agattcagtt tgattaccta tgtgatgttg aggttctgtt
     5821 ttttcagtac cttcatgaat gcttggttcg tcagccactg tattttcagt atcattttta
     5881 gcaacaccga ccgttgatgc agtagctaca cctgtttttt cagcatcttt aatatttgaa
     5941 gaagattttt cagcctcagt atgattagtt tcttcatcgt cattgacatg gtctaaataa
     6001 ttattaggct caagttcttc aaggtttcgt cttgttgtta aatatgcaat acctcggata
     6061 attaagctta aaccaatgta tcctactgca acgattgctg ttgaaatggc gatgactttg
     6121 attgaaaata gatcgccgat ttcttcgcta aacaagaaaa taagtccgaa tgtcattgaa
     6181 gcatggaatg tcgttgcaat ataaattgta cgtcctttag tcgctctaat taattcacca
     6241 agaatcatag agaatgagaa tgtataaagg aagttataag cagcaaattc tgtaccataa
     6301 gttgtatttg ctgagaaaac tgaatacatc aaaccaacaa caatacttgc aaagaacgtg
     6361 ttcattttcg tttcaacaat attttgtaag tatgagcgga atccgaattc tactacgaac
     6421 gccatcagaa tatgtccaat cagaatgtgt gtaataggta ctgataagcc tgttgattgt
     6481 aataaaataa agctatctgc aaatgtatta aagctgtaca taccaataat tagaataatt
     6541 aaaggtaaaa ttaaagctag caatagacgt tcaattactt tcaggctgat tgagaatttt
     6601 aaacctgcaa gttggacctt tttatatttg aaaacgagta tacaaatgat tgcagcaatt
     6661 aatggtgcta gatctgtaac ttcaaatata aagtgtttga caccaattat agactggaaa
     6721 tctctgagca taatggataa cgccattgta atgacaaaga agacgaaaat cgtcattgcc
     6781 cattgaaaac cagaaatttt attgttcttc attatatgta acctccatta ggtaacaagc
     6841 aaaatatctt ttagtaacaa ttatacatat tacaagccga gagataaatt gtctatgtca
     6901 atattaatga aaatgtaata gtcttgacgt attttattaa cataaatcag ctaagtattc
     6961 gttattacat agaaaatagg ggtaaaagta atgtagataa gtatttttaa ggtacggatt
     7021 taactatgaa ttgaattcaa aatttcctat acgttatact ttaattgtta ataagccaca
     7081 taaataagag ggggaaatgc tgtgtacaag caaggtgaac caaatttatg gactggaagg
     7141 ttagatagtg aaacagaccc gaaaaaattt agacattttc aaacagtaac atttgaagat
     7201 ttgtctaagc tggaaaagag tagtatgcca tcaggggtcg gtatattagg ctatgctgtt
     7261 gacaaaggtg ttgctttaaa caaggggcgc attggtgcaa aagaagggcc agatgcgatt
     7321 aaacaagcat ttgcaggttt gccggatttg aatcaatgtg aaactttagt cgattacgga
     7381 aatgtttatc atgatcatga ggaattaatt gatactcaaa aagaatttgc tatgcttgca
     7441 gcgaagtcaa ttgctaatca tagacaaaca tttttattag gcggtggaca tgatattgcg
     7501 tatgcacaat atttagctac gcgtaaagtt tatccaacac agtcaatcgg tgtgattaac
     7561 attgatgcgc attttgatac tcgtgctgaa caagaatcaa catctggtac gagttttaga
     7621 caaattttag aagaggatga caatactgat tatttagttc ttggtatagc tcaaggtggt
     7681 aatacgcaaa gtttatttga ttatgctaaa gagaaaaaga ttgattatgt ctttgcagat
     7741 gaattattaa accatgtgtc gccacctatt aaagatatga ttgagcgttt tgtgcatgaa
     7801 catgacgtca ttatgtttac gatttgtatg gatgtcattg atagtgcatt tgcaccaggg
     7861 gtaagtgcgc ctgcagtgtt aggtttgtat ccacatactg ttcttgaatt agcaaaacgt
     7921 attattccaa gtgataaggt gtcttcagtt agtattgctg agatgaatcc aacatatgac
     7981 gctgacaata gaactgctaa gctcgttgct aatttagtgc atcatttttt aaaataatta
     8041 aaaatattta tatgtgacga gatttgcatt gcagggcaga atgtgagtat ttcttaatag
     8101 aagttatatt aacgttgata tatttgaaaa ataattgcag taagaagact cttaagttgt
     8161 agtcatctta ctgcttattt atgtttgttg gtctgaatgt aatgatttat acatgtaaat
     8221 cataaaattg gttgtgaaat tgttttacag ttgactgagc aactgcataa gaggctctaa
     8281 taacttaagt taaaggcaaa aggtatacag ttgactgagc aactgcataa gaggttctaa
     8341 taacttaagt taaaggcaaa aggtatacag ttgactgagc aactgcataa gaggctctaa
     8401 taacttaagt taaaggcaaa aggtatacag ttgactgagc aactgcataa gaggctctaa
     8461 taacttaagt taaaggcgaa aggtatacag ttgactacgc aactgcataa gaggctctaa
     8521 taacttaagt taaagagcct ctaaaagtcc atataaataa tcttggcatt gttgtacgaa
     8581 tgtatgtact tgttcggagt gtttgcgttt tttatggtaa ttgatataaa tttttcggcc
     8641 taagtttgga cgaatttttt tatattctaa attagacgtg tgaaatgatt ggtaataaaa
     8701 tctcggaatg atagcgtaac ctaagccgag atgaacaaat ccaactgctg attcgaatcg
     8761 atctgtttct acaacgacat ttggacgaat atttcttcta ttaaaatagt catctaagtg
     8821 tttgcgcact tgagaatttt tgtttggtaa tatgagtggc aaattttcaa catctaccca
     8881 attttgattt ttaaatgttt ccttgggtgc taataaaatg taagattcct catataatgg
     8941 aatagatttt atatcttcgt gggttatttt ttcatttgtt atagctaaat gaatattaaa
     9001 attcagtaat tgctctatag attgatgttt atcatgtatt tcatataaac gatattgctg
     9061 ttcagggtag tcggaatggt gctttcgaat taaattcgca atccattgat tcgtagattc
     9121 aagagtccca atttttatcc ttggttctga tgtaacgctt aaatcataca ttttttccat
     9181 cgtggatcga tattgttgaa ctaattcgct cgcataacga taaaactgta ttcctttttc
     9241 ggtaatcttg atgtcttttg ttgaacgtgt aaataagtca taacctaaat ctgcttccat
     9301 ttttttaatc gtagcagtta aagatggctg gctaatatgt aaaaattgtg cagctttagt
     9361 aaaactatta tatttcacga tagccaagaa gtattctaac tgaataattt tcatgtgttt
     9421 tgctcctttt atttatagat tcaagctatg gataattagt ttatttatat ttgttggcta
     9481 tattttaaca tactataatg aatattgaag ataataatgt aagcgtttta aatttagtgg
     9541 aattaacttc taaaaagaac aggagcgaat tttatgagaa aaattcaagc aaaaaaaggt
     9601 ttgagtattg agtgtaaagg ttgggaacaa gaagcagtac ttagaatgtt atacaacaat
     9661 ttagacccag aagttgctga aagaccagaa gatttagttg tttatggtgg tattggaaaa
     9721 gcagcacgta attgggaagc ttttgaagca atagagaaaa cgttacgaga attagaatca
     9781 gatgagacaa tgttagtaca atcaggaaag ccagttgctg tatttaaaac acatgaagaa
     9841 gcgccacgtg tattaatttc aaattcagta ttagtgcctg aatgggcaaa ctgggatcat
     9901 tttaatgaat tagataaaaa aggtttaatc atgtatggac agatgacagc cggtagttgg
     9961 atatatattg gttctcaagg tattgtgcaa ggtacttatg agacatttgc cgagctaggt
    10021 aatcaacact ttaatggaga tttagcaggc actgttacac tgacagcagg tttaggtggt
    10081 atgggtggtg cgcaaccgtt agcaattact atgaatcatg gggtagcaat ttgcgtggat
    10141 gttgatgaaa cacgtgttga taagcgaatt gatacgaaat actgtgatgt taaaacagct
    10201 gatttagatg aagcattaaa attagcagaa gaggcgaaag aacgtggaga aggattatca
    10261 atcgggttag ttggaaatgc tgtagacatc catcaagcga ttctagaaaa aggatttaaa
    10321 attgacatta ttactgacca aacaagtgca catgatccgc taaatggata tgtgccacaa
    10381 ggatactctg tagaagaagc gaaagtattg cgtgaaaaag atccgaaaaa atatgttgaa
    10441 ctgtcacaag cttcaatggc aaagcatgtt gaattaatgc ttgaattcca aaaacgtggc
    10501 gctgtagcat ttgattatgg taacaatatt cgtcaagtag ccttcaataa cggagtgaaa
    10561 aatgcttttg acttcccagg ctttgtacca gcttacatta gaccattatt ctgtgaaggt
    10621 aaagggccat tccgctttgc tgcgttgagt ggtgatccaa aagatatcga gcgtgcggat
    10681 gaagaaatgc gcaaactttt cccagaaaat gaaaagttat taagatggct tgatttagct
    10741 gaagaaaaaa tttcatatca aggactacca tcacgtattg cttggttagg ctatggagaa
    10801 agagcgaaaa tgggcttagc tttaaatcgt cttgtacgtg acggtgaaat ttcagcacca
    10861 attgttattg gacgagacca tttagatgct ggttcagttg ctagtcctaa ccgtgaaaca
    10921 gaaagtatga aagatggtag tgacgcggtt ggtgattggg ctgtattgaa tgcactcatt
    10981 aacacagctg caggtggttc atggatttca ttccatcacg gtggcggtgt tggcatggga
    11041 tattcacttc atgcgggtat ggttgttgta gcagatggat cagagcgtgc tgaaagaaga
    11101 ttggaacgtg tattgacgac tgacccaggt atgggtgttg cccgacatgt tgatgctggc
    11161 tatgacatcg ctattcaaac agctaaagaa aaaggtattc atattccaat gattgataaa
    11221 gcaggtgata agtaatgaat gatttaataa ttaatcatat agcagaatta attttaccga
    11281 gatcaacaga taaacctttg aagggtaagg aattagatga attaaatgtt gtgaaaaatg
    11341 gtacagttgt cattaaagat ggcaaaattg tatatgctgg aacacataca gatgactacg
    11401 atgcgactga gacgattgat gctagtggga aggtagtgtc cccagcatta gtagatgcac
    11461 atacccattt aacatttggt ggatctcgag aacatgagat gtcattaaaa cgtcaaggta
    11521 agtcttacct tgaaatatta gaaatgggtg gtggcatatt atctaccgtt aatgctacta
    11581 gagaaacatc cgaagatgac ttgtttaaaa aagcagagca tgatttgctt actatgatta
    11641 aacacggcgt gcttgcagtt gaaagtaaga gtggttatgg attagataga gaaaatgaac
    11701 tgaagcaatt gaaagtgtct aatcgtttag ctgagaaata tgatttagac atgaaacata
    11761 ctttcctagg gcctcatgct gtacctaaag aggcaagttc aaatgaggca tttttagaag
    11821 aaatgattgc gttacttccg gaagtaaaac aatatgcaga ctttgcggat attttctgtg
    11881 aaacaggtgt atttacaata gaacaatcgc aacattatat gcaaaaagcc aaagaagcag
    11941 gttttaaagt gaaaatacat gcggatgaaa ttgatccgtt aggcggactg gaattagcaa
    12001 ttgatgaaca agcaatatca gcagaccact tagtagcttc tagtgataaa ggaaaagaaa
    12061 agttgcgaaa tagtgatact gtagctgtcc tcttacctgc aacgacgttc tatttaggta
    12121 aagaagatta tgcagatgca agaggcatgc tagacaataa cggtgcgatt gcattagcaa
    12181 ctgattataa ccctggtagt agtgtcacaa acaacttaca acttgttatg gcgattgcag
    12241 ccttgaaatt aaagctatca cctaatgaag tttggaatgc tgttacggtc aatgctgcta
    12301 aagcaataga cattaacgcg ggtacaatta acacaggtga taaggctaat ttagtaattt
    12361 gggatgcacc aaatcatgaa tatattccat atcattttgg tatcaatcac gcagaaaaag
    12421 ttattaaaga cggtaaggtc atcgtagata acacattatc ttttaaagca taaaataatt
    12481 agaaagctct aagcattggt ttacactaat gtttagagct tttgtggttt gtaaaaacaa
    12541 gtttatccga gaacacgttt ttgtatagat tcttaattat aaaagttaga atagtttgac
    12601 aacagtgtta tgatattatt aaacaactgt ctatcattag cgaaagtaat taacaagagt
    12661 acattgttgt gaaatcatcg tttaatagtc aaagatgatc atcgtagggt agacaacata
    12721 tcattgactt aaggaagggg aatgaccgat gggattaaaa gatcaagctg ttacctggag
    12781 aaggtatttt catcaatttc cagaactatc cgataaagaa tttaagacta cacaaaaaat
    12841 taaagatatt ttaacagaac atcatattag aatattagac ttaccacttg cgactgggct
    12901 tgttgctgaa gtgggacaag gtctgagttg tatagctgtt cgggctgaca ttgatgcttt
    12961 accgattcaa gagcttgttg aacaggattt taaatctgaa aatgaaggtg ttatgcatgc
    13021 gtgcggtcat gatattcaca tggctagtat tttggctacg gctgtaaaat taaaagaaat
    13081 tgagggcact cttactgggc gtgttaaatt catttttcaa ccagcagaag aactaggaca
    13141 tggcgctttt aaaattattg aaacacatgc attaaaagat gtacaagcag tattgggttt
    13201 tcataatgac ccatcgcgtt cggtaggtac atttgcaatc aaaacagggg caattacatc
    13261 agcagtagat cgttttgagt ttcatattaa aggcgtgggt ggtcatgctg caaagccaga
    13321 acaatgcaac gatccagtta ttgtgttggc gcaattgatt aatagtattc aatccatagt
    13381 tagtagaaat ctatctgcgt ttgatgaagc ggtagtaaca attggacaaa tatcatgtgg
    13441 taatacttgg aatgtcattg ctgatcatgc gtatgtacaa ggcacagtgc gtagttttga
    13501 tcctgtggta cgaaagcttg tagaaacacg attacaagat attgcggacg gattggctca
    13561 agcatataat atgaaaatta atttaaatta tacacattta cctggcgctg tgatgaatga
    13621 tgaggcattg acgcataaag caattgcagt agcccaacat gttggatata aggtggaaat
    13681 gatggaacag ccgcttacga ttggagaaga tttttcaggt tatagtcaac attttccaag
    13741 tgtatttgcg ttaattggtt ctcatagtga atatgattta catcatcctc aatataaacc
    13801 ggatgaaagg attcttgaaa aagtacctga gtattttgtt gaatttgtaa aaagactatt
    13861 gcatgagtaa ttcgagcttg tttttataaa gaagaaagta atttttagtg taatcatgat
    13921 taagttccta ggaagattgg gtaaataata accattaagc gaataaggag atgttaaaaa
    13981 atggcagctc aagatcctag aacaaaattt aaaacaacgg attatgaaaa acaagaacaa
    14041 gaggtaccgg gtttacaatc tgaaatgacg ccagcacctg attgtggcga aacgtcttac
    14101 caaggtcatc aacgcttaca aggctataaa atattagtca caggtggtga ctcagcaata
    14161 gggcgtgcag cagcaattgc gtatgctaaa gaaggtgcag atgtagcgat taactatctt
    14221 ccgagtgaag aacaagatgc acaagaagta cgccaagtga ttgaagaaag tggtcaaaag
    14281 gcagtgttaa ttcctggtga tataagagat gagcagttca actatgacct tgttgaacaa
    14341 gcatatcaac aattaggtgg cttagataat gttactttag ttgctggaca tcaacagtat
    14401 catgatgata ttcatggatt tacaactgag gcatttacag aaacatttga aacgaatgtc
    14461 tatccgctat tttggacagt tcaaaaagca ttggaatatt taaaaccggg tgcgtcaatt
    14521 acaacgacat catctgtaca aggttataat cctagtccta ttcttcatga ttatgccgct
    14581 tcaaaagcag caattatttc attaactaaa agtttttcag aagaattagg accaaaagga
    14641 attagagtga actgtgtagc gcctggtcca ttctggtcac cattacaaat ttctggtgga
    14701 caacctcaaa gtaaaatccc aacatttggt caaaaaacac ctttaggtcg tgcaggtcaa
    14761 cctgttgagt tatgtggaac ttatgtgcta ttagcgtcag aagaatcaag ttatacaact
    14821 ggacaagtgt tcggtgttag cggtggagtg caaatagatt aggtttgtat gaagctttta
    14881 attgaacaac ataatattaa tttagttact tcaatccatt aaattaaaat gaattggtga
    14941 agtgtatttt aaatagtcgg taaaggtgtg tatctttatc ggcttatttt aatactctgt
    15001 cataaggagc gtttgctcag ggtgtggatt ataaggttta agcagaataa actttcttaa
    15061 tgaggatgtc aggacaacaa cgaaaaaatt aaaaaatgtt gcgggattgg gtttagggac
    15121 ggttagtgga agaaagttaa attcattaac aagtgaaata cctaaggcta gatattaaac
    15181 gtcagaacgg acaaagaata ataggatatg ttttcaatta aatagcgaag tatttatatg
    15241 ttaagatgat gtaaattaca tagaaacgct acccgtagaa gtactgatca tagaaacgct
    15301 gataaacgta aatttgcaaa ctcgatatta aacaaagggg ttgttattat ggcattaata
    15361 gtatctagag ctatattatt tttgatttta gcattagctt ttggaatagc gactgttttt
    15421 cctgaacaaa aattaccatt atatatagta ttcacattat ttgtagcagt tgcgatcgtt
    15481 tcgaatgtat ggtttacaca tcaaaacgtt aaaaagcata tcattaagtt taacaaatac
    15541 gtcgcatact ttttatcctt tgtctcaggg ttactttaca ttggatttgt aatttataca
    15601 ttaactatag ggagctatga tgttttacca attattatat ttgcaataag tatcgtattg
    15661 gttcaaatat tagaaaatat actgggaatc agtataaaaa atacgcgtct atcaaacaaa
    15721 cagttgaaag tcatctccaa gcaacgtgtt cagcaattta aagtcatatc attaatatta
    15781 gtaagtgtgt ctatcgtgtt catttttatt gcattaatgc ttaaatggtg gcttttactt
    15841 gttattagca ttattgtttt gattattatt acagtagcaa tgattgttta tgaaaaatat
    15901 tatacgactc ctgattaatt tatgattgac gtactaagga ttatcttgta acgtaaacgt
    15961 aaataagcat cacttatcta gagaggtgga gggactggcc ctatgaagcc tcggcaacat
    16021 ctcgaatgtg ccaattccag taaccgtaat ggtttgaaga taagcaggta aagcacatga
    16081 aaacctcttt cttcatcgtt tgtgagaaag aggtattttt aattggaaag caggtaaaaa
    16141 ggatggaagt acataaaaag agcaatgctt gggcattatt ccccttgtta ttatttgtgg
    16201 cgttgttttt aggcgtaggt attatcacag gtgattttac ttcaatgcca ttaaatgttg
    16261 caattacgat aacggtaatt gtggcgttat taatgaatcg aaaagaatca tttgctaaaa
    16321 aagttgaggt cttcacgaaa ggtgcaggcc attcaaacat tattttaatg atgttgattt
    16381 ttattttagc aggtgcattt tcaaatacag ctgaaaaaat gggtggcgta aagtcgacag
    16441 ttaatttagg actatcatta atcccagaaa atttaattat tgtaggattg tttgtgattt
    16501 gtatgtttgt ttctatatct atggggacgt ctgtagggac tgttgctgca atagcacctg
    16561 ttggttacgg atttgcacaa gcgacagatg taccaactgc gctagcaatg gctacagttg
    16621 ttggaggtgc aatgttcggc gataatctat caatgatttc agatacaaca attgctgcgg
    16681 ttagaacaca acatacgaaa atgaaagata aattcagagt aaactttaaa attgtattgc
    16741 caggtgctat tttaacgatt attattctgt atttcttaac gaatggcatt tctttaaatc
    16801 atgctaaaag ctatgattat aatctaatta aagttgtacc gtatatttta gtattggtat
    16861 tagcattatt aggtgtaaat gtcattatcg tattgattgg cggaacatta ttagccggaa
    16921 tcattggtct catagatggt tcattcggtt ggatggggct actagatgca gtgtccaaag
    16981 gtattataag catggaagat attgctatga ttgcgttgct aattggtggt ttagtcggta
    17041 ttattcaaca caatggtggt attgaatggt tgttgcaatt tgttcgctct aaagtgaaat
    17101 caaagcgtgg cgcagaatta ggaattgcga gtttggtaag tgtcgcagat attgccacgg
    17161 caaacaacac catctctatt attatgtcag gtcctttagc taaaaatatt gcagatgaat
    17221 atgatgtaga ttcaaggaaa tcagcaagta ttcttgatat atttggaggc tgcttccaag
    17281 gtttattacc atatagcccg caagtcattt cagcggctgg tgtagctggt atttcaccgt
    17341 taatgttatt cccatacagc atttatccta tattattagg tgtatgcgga cttattgcga
    17401 ttttatttaa ttttccaaag ctaaaaaaga acactagtca agaaatgaaa cattaatgct
    17461 taacaatata tcgatgtagc caagtaaggt ggcttgttat attaacagat catgttgtac
    17521 tcaccgtata acgctaatct tttaaaaatg atgatcatct atgtttggaa ctgagcaaaa
    17581 atacgttaaa ataaaagtaa gagcgaaagt gggtggacaa cagtggcaaa atttaatgta
    17641 gagaatgaac atgtcgaagt tgaagttgaa aagctttata aattttctcc agagttagta
    17701 tacgaagctt ggacaaagaa agatttgttg aaacaatggt ttatgacatc ggccagaacg
    17761 aataaagaga ttgaagcgga cgttaaagaa ggcggtaaat atcgtattgt agatcaacaa
    17821 cgtaatggca aagtaaatgt gattgaaggc atttatgaat ctttagttat ggatgaatac
    17881 gttaagatga ctatcggtat gcctggacta agtgaaacac aagatgtcat tgaggtagaa
    17941 ttttttgaac gtgaaacagg tggcacccaa atgctatttt attatcgttc attagttgaa
    18001 aaagaaagac gatttactaa tttagaatac aagcaaaaga aaaaagaata tcatgatgcc
    18061 atggtgcatg gtttcgaatt gatgtttgat aagatgtacc acgttatcga aacatcaaca
    18121 caacaataaa atgataaaag gaagttatga taggctcaag gccattaaat cataacttcc
    18181 ttttttctaa tttgaaatat cattatgtag taaatactca ttatattttc ttacaagtgt
    18241 ttcaaagaat atatgataac tgatgaagct agaatagtct gtaccatcag ttaagtagaa
    18301 tgtgttactg gatacataga cgttgtaatt tgctttttga gcaagggtat tatctttcat
    18361 tgtagtaaca gaaataaagt atttttgtct aagttgaagc aagtttgtta cttctaataa
    18421 ttcatgtgtt tcacctgata atgaaataat aaagaataaa tcattaggtt cactcttgtt
    18481 caaaatcatt ttaagttcat gttcatcatg aagtacaatg acatgattat gtaacgttaa
    18541 caatatacgc tgtgcctctg aagcaacgtt taactgtgca cgccctgtac catacaaata
    18601 tattgttgaa gctgtatgca ttttttcagt aagaagctgg taatcaacgc gttctaaata
    18661 actgaatgta ctatcgattt cttgtttgaa actatcgata gagttagaag gtaattggtg
    18721 tgtcttatta ctttcaaatt taaggtacga tttaaaatca ctataaccgt caaaacctaa
    18781 tttgcgtgta aatctgtgaa tggttgcatt cgaggcatgt gtgaattgcg ctaaatcttg
    18841 tattttcata tttttacatt catctatatg tgtattgata aagtgagcga tatgtaaatc
    18901 attatcatta agtttatcaa agtttcgatt aatgtgttca tctaaaaaca tattcaacct
    18961 ccttgaaaat aaatgtgtga tagatcgtta ttacattaaa atcttgtatg tcattatata
    19021 tatttgtgta ttgatatagg tttgattatg cgtttaaaac gaatgtatta caaatacaac
    19081 gatgtcatat ataaggtcgt gaaaaaattt tcactatatg aaaagataat ctgattatac
    19141 aaaatacttt caaaatacgt caatgtcgtt gtaggtgtgt aagcgtttac tatatattat
    19201 atttaaatag taagacatct atttataaat gaatctaatt agatgtattg ttaggaaaga
    19261 tgatatggag ggaatagaag atgaatgcga taaagcgttt cggtagtgca atgattgtac
    19321 cggtattaat gttcgctttc tttgggatag tgcttggctt tgcgacatta tttaaaaatc
    19381 ctacaatcat ggggagttta gcagatcaac atacgttttg gtttaaattt tggtcagtaa
    19441 tagaatctgg tggctgggtt atttttacgc atatggaagt tgtatttgtt gtaggtttac
    19501 cactttcttt agctaaaaaa gcaccaggtc atgcagcact tgcagcattg atgggttact
    19561 taatgtttaa tacatttata aatgccattt taacgcaatg gccacatacg tttggcgcta
    19621 atttagaaaa aggcgttgaa aatgtacctg gtttaaaatc aattgcaggt attgccacat
    19681 tggatacgaa tattctaggt ggtattatta tttcagctat tattacttgg attcacaata
    19741 gatactatag taaacgatta ccagaaatgg ttggtgtatt ccaaggttta acatttgtag
    19801 ttacgatttc attttttgtg atgttaccat tagcagcaat tacttgtgtt atttggccaa
    19861 ctgttcagca cggtattggt tcaatgcaac actttatcat cgcttcaggc tatatcggtg
    19921 tttggctgta ccacttctta gaacgtgtat taataccaac aggattacat cactttattt
    19981 atgcaccaat tgaagttgga ccagtagtag tcaatcacgg cttgaaagct gaatggttac
    20041 aacatttaaa tgagtttgct aagagtacta agccattaaa agagcaattc ccttatggct
    20101 ttatgcttca aggaaatggg aaagtatttg gatgtttagg tattgcttta gcaatgtatg
    20161 ctacgacacc taaagaaaat cgtaaaaaag tagcagcatt acttattcct gcaacattaa
    20221 cagcagtcgt tgtaggtatt actgagccat tagaatttac attcttattc atcgcaccat
    20281 acttatttgt tttacacgca gttttagcag catcaatgga tactttaatg tacgcatttg
    20341 gtgttgtagg taacatgggt ggtggcttgt tagactttat ttcaacaaac tggttgccat
    20401 taggtaaaga acattggggt acttatgttg cacaagttat tatcggtctg atatttgtag
    20461 cgatttactt cttcttattc agattcttaa ttttgaaatt tgatattcca ttacctggac
    20521 gtaagaaaac agaagaggaa gttaaattat tttctaagca agattacaaa aataaaaaag
    20581 gtgatagcgt agattctaaa cgtgcttcaa caggtaatga atacgaagac aaagctgcgt
    20641 attacctcga cggtttaggc ggcaaagaaa atatcaaaga tgttactaac tgtacaacaa
    20701 gattacgctt aactgtttat gatgaaagta aagttgcgga tacggaatat ttcacgcatc
    20761 aacaaatggc acacggatta gtaaagagtg gtaagagtat ccaagttgta gtaggtatga
    20821 cagtaccaca agttcgtgaa gcatttgaac aaatggttga ggatcaatcg tccgaagata
    20881 aataatacaa aaaaagacac atcactatac gcaatcattg taaatgatca tcgtatagat
    20941 gtgtcttttg ttatgatata cgagctttgc tcatagatga cgatctataa ttggcagata
    21001 gaatgtcgat atttctagtt gcaatcgtat gtaacaattt agcaaatatt tctggcgtac
    21061 atataatatg tgcatgagac ttattcaacg tataagtgat atcagtgtga ttttggaaca
    21121 tattcataat atcgatgact ttgtctttaa tgaaagcatc ttgcaagcca tcaatattga
    21181 tatcgaaacg ctttgcagat tgatataaat tatcagaatc aagtaattca tttattttct
    21241 ttggtgaaag tacttggaaa atactatact gatgaaaact taaaaatgaa atgaagtatg
    21301 gaagttgttg ctttggtaag ctaatttcta atacatactg attaaactgt ttgtttattg
    21361 agaattgcaa cgcatcacaa aattgtacac ttttgcataa cttacttaat ttttttctag
    21421 tagtacggtc atttaaaccg gcgatgttga tgatgtatgt ttcgaattta tcaattaaca
    21481 tgtgaggacc tcccgaggaa tacatggcat taaatacacg tttaatattt ataaaggtga
    21541 cttaattttg ttcaagttga ttttaccacg ctttttttct ttattcacta agacttttga
    21601 atgaagttta aaataatttt ttatcagtga taaaatattt gcaataagaa gagaatgact
    21661 aaataatctt aattttcaga aaagtaattg taaccttact ggtcttatgg taatattttt
    21721 caatattatc gacgaggatg tgttaacaat gtttcaaaaa ttgagtatat ttgccacgaa
    21781 gagttttttg gtatggatgt tagtagcagc tgttattgga tttatttccc cacaacatgt
    21841 tgcgacatta ggtaagtggg taccttattt acttggtatt gttatgttag gtatgggatt
    21901 aacaattaca cctaatgatt tcaaaatggt ctttaaagca cctagagcag taattattgg
    21961 tgtctgtcta caattcagta ttatgcccac attagcattt ataattgcaa agtcttttca
    22021 tttaccacct gatattgctg ttggcgtaat attagttgga tgttgtccgg gtgggacatc
    22081 aagtaatgta atgagttatt tagccaaagc taacgtagca ctttctgttt ctattacgac
    22141 ggtctctacg ttgctagcgc cattcgttac acctgcgtta atatatctat ttgcaaatga
    22201 atggttggaa gtatctttct tgagtatgtt gtggtcagtt gttcaagttg tattaattcc
    22261 aattgcttta ggtattgttt tgcaaattat taatcgtaaa attgctgaaa aagcttctac
    22321 agctttgcca attatatcag ttgttgctat ttcattaatt ttagcaatag ttgtaggtgg
    22381 cagtaagcac caaatcttaa ctacaggatt attaatattt ttagtagtta ttttacataa
    22441 cgtattagga tatacgattg gatattggtt agctcgtctt ttaaaattag atcgacaaga
    22501 tcaaaaagca gtcagtattg aagttggaat gcagaactct ggtttagctg tgtcattagc
    22561 agcattgcat tttaatccaa ttgcagcagt accaggcgca gtgtttagtt tcattcataa
    22621 tataacaggg cctattttag caaagtattg gtcaaaaaag ttataattgc actaatagaa
    22681 tgaagtggtc atcggactgt gttaagcttt gataaagaga aaaaatagag gagtaaatat
    22741 atgtatagag cagttatatt tgatttcgat ggaacaataa tagatacgga acaacattta
    22801 tttaatgtta ttaataaaca tttagagatg cataatgccg atcctataag cattgatttt
    22861 tatcgttctt ctattggagg agcagctaca gatttgcatg accatttaat taaagcgatt
    22921 ggttcggaaa ataaagataa actttatgaa gaacatcatc ttactagtac aacattaccg
    22981 atgattgata cgattaaatc attgatggca tttttaaagc aacgtcacat tcctatggcg
    23041 attgccacaa gtagtgtgaa agcggaaata atgcctacct ttaaagcatt aggtctagat
    23101 gattatatag aggtagttgt tggtagagaa gatgttgaac aagttaaacc tgatcctgaa
    23161 ttatatttat ctgcagtaca acaattaaat tatatgccga cacaatgttt ggctattgaa
    23221 gattctgtaa atggtgcaac agccgcgatt gcagctggat tagatgttat tgttaatacg
    23281 aataaaatga caagcgcaca ggacttttct aatgtagatt atgtagcaaa agatattgat
    23341 tacgatcaaa ttgtagcgcg tttctttacg aaataggagg cgtatcatga tgggttacat
    23401 tatattgttt tttctagctg gtccagtaat tttaggcgtt ggaaatttgg tgattggtcc
    23461 tatatttaac aaacagacac catttcgcgt gcaagtaaga tcttttgttg ttggttcaat
    23521 gatttactta atactcgcaa caattggcta ttttttacta ttacaaggta aactttaacg
    23581 agaaaaccac cttacctcat taaatggacg accatatgta tgtgaaatgg tagaacgttc
    23641 atgtttatgt atgagatagg gtggtttaaa tagttacata tattttaata ataacgtcac
    23701 gatgataagt acaattaaga taatatctat gcctactata attgtagctc ttgttgcatt
    23761 acttccttgt tcttttgctg atttcatagc acggtagttt ggcacaaagc taataattag
    23821 taagattaat acaattacac caattaatgc tgttgtcatg atgaacgacc tcctttattt
    23881 ttttcaatca attcccaaat aaacgtagca atcacaccga tgattaaccc agtgatgaca
    23941 ccgatttgta ccgttaaaaa tatggcgccg aaaatgatac caataattaa tgcaatcggt
    24001 aaagtcgtac cgagtttaat cttgcgctct ggagaattaa taatagtaaa tactgtaaga
    24061 caaatgagta tgaaagcaag tgttgcaata atagttcttc caactaaata taggatgtca
    24121 ggtttttcca taccgatata atttatgatg aaaaatgcta cagcaaagag taccgatatt
    24181 tttgtagcac gtagcagtat ttgttttaac attgatatac tcctttttaa tattattaaa
    24241 attatatcat aattaccaag agtagctgaa gttgtatgtg actcaacggt tcacggacaa
    24301 cttttttaat tttttagaaa aatcacaaaa taattgtttg caaagttgca aaagcctgct
    24361 atagtagttc tgtaaacgat tgcatggtat gcaaatatta atgtaccaaa atcgataatt
    24421 tatagtataa ttacggcaat aagttttttt atggatttat ttagtatcaa tcagagatgg
    24481 ggtaagaagt tatggagaac aatgaactac aaaggggatt gagtgcccgt caaattcaaa
    24541 tgattgcact tggtggtacg attggcgtgg ggcttttcat gggtgcgaca agtacaatta
    24601 aatggacagg cccatcagtt atccttgcat atttaattgc gggtatcttt ttatttttaa
    24661 tcatgagagc aatgggggaa atgatttatt taaaccctac aacaggatca tttgcaacat
    24721 ttgcaagtga ttatatacat cctgcagcag gttatatgac agcatggagt aatatattcc
    24781 aatggattgt agttggtatg agtgaggtca tcgcagtagg agaatatatg aagttttggt
    24841 tcccggaatt gccaacttgg attcctggtg ttattgctat tttattatta atggcagcga
    24901 atttattctc ggtaaaagcg tttggagaat ttgaattttg gtttgcttta attaaagttg
    24961 taacaattat tttaatgatt attgctggtt ttggtcttat tttctttggt tttggaaatg
    25021 gtggccatgc ggtaggtatt tctaatctat ggacaaatgg cggatttatg ccaaatggta
    25081 ttgttggttt cttctttgca ttatcaattg taattggttc ataccaaggt gtggaactga
    25141 taggtattac ggcaggtgaa actaaagatc ctcagaaaaa tatcgttaaa gcagtgaatg
    25201 gtgttatctg gagaatttta attttctatt taggtgctat ttttgttatt gtttcagtgt
    25261 atccttggaa tcaattagga gacattggaa gtccgtttgt tgcaacattt gctaaaatcg
    25321 gtattacatt tgcagctgga ttaattaact ttgttgtatt aactgcagca atgtcaggat
    25381 gtaactcagg tattttcagt gcgagtcgta tgatttatac acttgctcat aaaggtgaaa
    25441 tgccgaaaat atttactaaa ataatgagaa atggtgtacc attgtacact gttatagcag
    25501 tatctcttgg tattttaatt ggtgctttat taaacgtaat tttaccttta tatatcgatg
    25561 gagcgaagag tattttcgta tacgtttata gtgcgtctat tttaccaggt atgattcctt
    25621 ggtttatgat tttattcagt cacttacgtt ttagaaaatt acaccctgaa gaattggaag
    25681 gtcacccatt caaaatgcct ggtggcgcag taactaacta tttaacaatc ttattcttaa
    25741 tattagtatt ggttggtatg gtatttaatg ttgaaactag aatatcagtg ctaattggtg
    25801 tcatcttctt aacgattgta acgatttatt actttattag atataataaa aataatgtaa
    25861 aagcaaaata gtatgattat aatgtgcgct tattttatga caaatttggc cctagcttat
    25921 taaagttaaa tagccaaaga atgttatgag taggcgcatt tttttataaa tatagcttaa
    25981 atcgaagttg tagtgtgaaa aacttataag tggcaatatt atcgttagtt tttactgtgg
    26041 gctttaaaat aaaaaatagc attattaaaa taacaaacgc aattgatata tggaatgaat
    26101 ccgttaatca actgcgtttg tttttaatat aagaaattga atggccttat caaatcattg
    26161 gtcatatgcg tatgttatac aagataaaag acatgtaagc tttgaaaatg atgcataaat
    26221 tattatatga catgattaaa gaatttatat aagactgtta atgtttaaag ttaatatttg
    26281 ataaatgttt acgatagtta tctaaagcca ttttagattg tgttatgaaa tctaatgatg
    26341 cgtgataatt taatgcaaca taacgataat ataaaatatc aatagtgaac atttgagtaa
    26401 ataatgaagt tgttgctccc atatgcatct cattttcatc agttttccca taagttaaaa
    26461 caatatttga tgcctgtgct acgggattat cccttgtact agtaattgta attataggta
    26521 tatggtagtc atcaataact ttaaccattg attgcatttc actttgcgta ccattgttag
    26581 taataagaat aacactgtcg ttcgaattgt gagttgctaa taatgttgca aaaatatgtg
    26641 tttcttgaac aagttgaata ttaagaccta ttcttgataa cttttggtat aagtcggtag
    26701 caactacaaa agatgcgcca aaaccatata taaaaatcgt ctcagaacgt tttaaacaat
    26761 gacatatttg atcaatagtt ttatcattta attcgttatt agcatgatta agtgcgcgtg
    26821 tagtacgtgt atggagttta gttcttaaag attctgtgct ttcgttattc attaattcaa
    26881 cattgtaaat tgatgatgct ttaggaacat atttagatat atttattttc aagtcgtgaa
    26941 aaccgccatc agtaattttc ttgctgaatc gaatgactga tgatgtacta atatctaata
    27001 agcttgctaa gtcttgagac ctcattttaa tcaccttttg aggtgaattt agtataaatt
    27061 tagctatctt tttttcattt ttagtcatat atggatattg actatctatt tctgttagta
    27121 cgtttgacat attaatcact ccactttaac gcaatatatt ttattagcgt tggctattgt
    27181 tgtttaacgc gatggtattt acaacttgta acgtaagaga atgaaaaata agctgaatat
    27241 gttgtttgtt taatgatatt gaattataaa actgtaaata tttttaagat gtatagataa
    27301 aaaatagcat actcccatgt gcaaataata tatcaatata ttgatatatt accgtaagtc
    27361 atactgtata ttcccaattt atacattatg tactcatcta tttaaatctt agtaaatgta
    27421 gttatataag actaaatcta ccatcttaat cattatgatt ataactcgat taatgcatct
    27481 ttacaatttg aagaaacttt ttaattgtat aattta
//
LOCUS       DBNENW010000019        25535 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNENW010000019 DBNENW010000000
VERSION     DBNENW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.019"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(98..295)
                     /locus_tag="AC1LW8_000650"
     CDS             complement(98..295)
                     /locus_tag="AC1LW8_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4772515.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(798..2138)
                     /gene="glnA"
                     /locus_tag="AC1LW8_000651"
     CDS             complement(798..2138)
                     /gene="glnA"
                     /locus_tag="AC1LW8_000651"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIP4772516.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2157..2525)
                     /locus_tag="AC1LW8_000652"
     CDS             complement(2157..2525)
                     /locus_tag="AC1LW8_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP4772517.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2769..4007)
                     /locus_tag="AC1LW8_000653"
     CDS             complement(2769..4007)
                     /locus_tag="AC1LW8_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4772518.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4026..5264)
                     /gene="hflX"
                     /locus_tag="AC1LW8_000654"
     CDS             complement(4026..5264)
                     /gene="hflX"
                     /locus_tag="AC1LW8_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIP4772519.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKMEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NCPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5376..5852
                     /locus_tag="AC1LW8_000655"
     CDS             5376..5852
                     /locus_tag="AC1LW8_000655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIP4772520.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6074..6307)
                     /gene="hfq"
                     /locus_tag="AC1LW8_000656"
     CDS             complement(6074..6307)
                     /gene="hfq"
                     /locus_tag="AC1LW8_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIP4772521.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6322..7257)
                     /gene="miaA"
                     /locus_tag="AC1LW8_000657"
     CDS             complement(6322..7257)
                     /gene="miaA"
                     /locus_tag="AC1LW8_000657"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIP4772522.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7275..8189)
                     /locus_tag="AC1LW8_000658"
     CDS             complement(7275..8189)
                     /locus_tag="AC1LW8_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP4772523.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8339..10012)
                     /locus_tag="AC1LW8_000659"
     CDS             complement(8339..10012)
                     /locus_tag="AC1LW8_000659"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIP4772524.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(10170..11666)
                     /gene="glpK"
                     /locus_tag="AC1LW8_000660"
     CDS             complement(10170..11666)
                     /gene="glpK"
                     /locus_tag="AC1LW8_000660"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIP4772525.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLSRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11795..12613)
                     /locus_tag="AC1LW8_000661"
     CDS             complement(11795..12613)
                     /locus_tag="AC1LW8_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIP4772526.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(13088..13630)
                     /locus_tag="AC1LW8_000662"
     CDS             complement(13088..13630)
                     /locus_tag="AC1LW8_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HIP4772527.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            complement(13636..15645)
                     /gene="mutL"
                     /locus_tag="AC1LW8_000663"
     CDS             complement(13636..15645)
                     /gene="mutL"
                     /locus_tag="AC1LW8_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIP4772528.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMVENQNDEIVIR
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            complement(15658..18276)
                     /gene="mutS"
                     /locus_tag="AC1LW8_000664"
     CDS             complement(15658..18276)
                     /gene="mutS"
                     /locus_tag="AC1LW8_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIP4772529.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            complement(18579..19070)
                     /gene="thiW"
                     /locus_tag="AC1LW8_000665"
     CDS             complement(18579..19070)
                     /gene="thiW"
                     /locus_tag="AC1LW8_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIP4772530.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            complement(19097..19462)
                     /locus_tag="AC1LW8_000666"
     CDS             complement(19097..19462)
                     /locus_tag="AC1LW8_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HIP4772531.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            complement(19463..21007)
                     /gene="miaB"
                     /locus_tag="AC1LW8_000667"
     CDS             complement(19463..21007)
                     /gene="miaB"
                     /locus_tag="AC1LW8_000667"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIP4772532.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            complement(21141..21434)
                     /locus_tag="AC1LW8_000668"
     CDS             complement(21141..21434)
                     /locus_tag="AC1LW8_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HIP4772533.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGMHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            complement(21528..22394)
                     /locus_tag="AC1LW8_000669"
     CDS             complement(21528..22394)
                     /locus_tag="AC1LW8_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIP4772534.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            complement(22395..24155)
                     /locus_tag="AC1LW8_000670"
     CDS             complement(22395..24155)
                     /locus_tag="AC1LW8_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIP4772535.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGIKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEVKGKIIATEIKEMV"
     gene            complement(24294..25091)
                     /locus_tag="AC1LW8_000671"
     CDS             complement(24294..25091)
                     /locus_tag="AC1LW8_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIP4772536.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            25265..25480
                     /locus_tag="AC1LW8_000672"
     CDS             25265..25480
                     /locus_tag="AC1LW8_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772537.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT         7784 a         4830 c         3484 g         9437 t
ORIGIN      
        1 tttctttcac ttttagaaaa cttttaatac tataaaaaca cccactcagt cactagtttg
       61 ggcagttatt gtatgcctat tgaactcaat gcttatatta caataccttt ttcgcatatt
      121 catataagat ctttgcattc tttaagctta aattttctat tcttctttcc ccacggcgca
      181 tagcattaat attaccgtaa cttatcccag tatctttatt aatttgataa cttgatatct
      241 ctttgttttc tatcaatttt ttgattgtat tgaatatttc atcatagcaa ttcataaatt
      301 agatgaggcg aaatttttaa ttttttagaa tatcaatagt agtataacta aaatgaaaat
      361 accgatcgat aaacaaaaag atattttttg ttttgtttct cttttcatat agtattaccc
      421 ccttaataat gagtagtaag gtccctcttt tcggcgtctt accttagaac gttctgcaaa
      481 tgaattcgat gagaagtaat atgatatggc tattttcaag taatactcaa cgttttcgcg
      541 acgttctttt atcgcctcat ctcatcacct ccaaatatat taaaattcat gtgaactaaa
      601 atataaaatg gtcttcccca gctttaaaaa aataaataca caaaacattt acttgaccaa
      661 aacttgacca aaagttgtcc aatcgtaaca aaaggcacat tttacaccca aaaaagttgc
      721 ccagttaaat caaaaaagag ctaaagcaaa acgctctagc ccttgatatt actgatttcc
      781 catcattgtt tagcgtatta atattgcttc atgtactgat ctctttccca ttcagatact
      841 tgagttctgt agtaatccca ttcaattgat tttgaattaa taaattgatt atagatatga
      901 tttcctaaag ctttttttat aacttcattt tcacgcattg cttttaatgc tgtgtaaagt
      961 gttgaaggta agtcttgaat gcctactgct tcacgttctt cacgattcat ttcgtaaata
     1021 ttttggttaa caggttctgg aacttttaat ttatttttaa taccgtctaa accagcttct
     1081 aaaattgcag ctaacgccat atatgggttc gcagctgggt caactgaacg tacttcaata
     1141 cgagttgata atcctcttga agatggtaca cggattaacg gtgaacggtt tttaccgctc
     1201 catgcaatat aacatggtgc ttcataacca ggaactaaac gtttgtatga gtttactaat
     1261 gggttacata ccgctgtaaa tccacgtgcg tttttaagta caccagctgt gaattggtaa
     1321 gctgtttctg ttaagcccat ttcagtattc ggatcaaaga atgcattttc tttacctttg
     1381 aataatgaaa cgttaaagtg cataccgcta ccattcacac caaataatgg tttaggcata
     1441 aatgttgcgt gtaaattatg tttacgtgcg attgttttaa caaccaattt aaatgtttgg
     1501 atattatcac atgctgtaac agcatctgca tatttaaagt caatttcatg ttgaccaggg
     1561 gcaacttcat ggtgactagc ttcaatatcg aagcccatat cctctaattc taaaacaata
     1621 tcacgacgac agttttcacc taaatctgta ggtgctaaat cgaaatatcc accatcatca
     1681 ttaagttcta aagttggttc ccctttttca tccaacttaa acaagaagaa ttctggttca
     1741 ggccctaggt taaagtctgt gaagcctaaa tcttccattt cttttaatac acgttttaag
     1801 tttgcacgag gatccccttc aaatggtgtt ccatctgttt tatatacatc acaaattaaa
     1861 cgtgcaactt taccttgtcc agcagtccat gggaagatta cccaagtatc taaatctgga
     1921 tgtaagtaca tatctgattc ttcgatacgt acgaaacctt cgatagaaga accgtcaaac
     1981 atcatttcgt tatcaagtac tttttctaat tggcttacag gcacttcaac atttttaatt
     2041 gttcctaaaa tatcagtgaa ttgtaatctt aaatatctta cattttcctc ttctgcaaat
     2101 ttacgaatgt cgtctttagt gaaagtacgt tttggcattt taaaatcctc caaattttat
     2161 ttaataaatc gggataaatc accacgattt attggcaaag tttctccaat aggcttttgc
     2221 gtggcatcta caatcatctt ttttcttatc tcttgttcat ctgttgttaa atgctcttgt
     2281 gagtcataaa tgatttgttt aatccctttg atattaaatc ctttttctaa taatgattta
     2341 atttctagta atctttccaa atcattgagt gagaacagac gtttttgacc ttctgttctt
     2401 tcaggtttga tgagttcatg tgtttcatag taacgtattt gccttggcgt taaatccgtt
     2461 aacttactta ctacactcat agagaagaca gccatatttc gtctgattgc atcattcgat
     2521 atcattgttc ctctcctcta cttttgaact tgttaataac ttatcataat ttttttagga
     2581 tttcaaaaat atgtcaggtt ttctgacata aagctcaaaa cattgatata actgcaattt
     2641 tgacctttaa aacgccataa aacaatctac agctaaaata ttttttcaat ttttaaaaat
     2701 aatctgataa atctccgcaa aaaaatttca cccaaaacac attatcattt agggtgaaat
     2761 ttaatctatt atataagtcc ttgttctttc aactgattaa cagctcttgt cacagcaatt
     2821 ttaacgtgtt catatgttaa tcctccttga acatatgctt cataaggagg acgaataggt
     2881 ccatctgcag ataattcaat cgatgaacct tgaataaacg taccagcagc cataataaca
     2941 tcatcttcgt aaccaggcat ataactcggt tctggactaa aatgtgcatt aattggggat
     3001 gcgtgttgaa tactttgaca aaatgaaatc atttgttctt tcgttttaaa tttaactgtt
     3061 tgaattaaat cagttcgttt ttcgtagtac ttcggcgttg tgttcatatt cattttttct
     3121 aaaaataaac tagtaaacaa tgccccttta agactctgac tgacaacatg tggtgctaaa
     3181 aagaaacctt gatacatttc aagcaatgca tttaatgatg cacctgcttc tttaccaata
     3241 ccaggtgctg tcaatctata accacatcgt tcaattaaat cttttctacc agcaatgtat
     3301 ccaccaatct tagctaaacc accgccaggg tttttaatta atgatcctgc tattaaatcg
     3361 gcaccacatt ctataggttc acgtctttca acaaattccc cataacagtt atccacaaat
     3421 attaaaatat taggatgcac ttctttcaac ctagtaatca cctgttcaat ttcatctaga
     3481 ggaattgaag gtctttgatc atagcctttc gaacgttgaa tcgctattac tttggtacgc
     3541 tcagaaaccc catctaacac actttcaata tcgatcttac cttctttaag tgcaatatct
     3601 ttatacgata caccgtgctc cattaaactt tcaataccat ttccgtttac accaatgact
     3661 tcaagtaaag tgtcatatgg acttcccgtt atataaatta attcatcacc atgttttaat
     3721 aaactttgta atgcaatagt aatcgcatgc gtacctgaaa taatttgcgg acgaacaatt
     3781 gcatcttctg ctttaaatgc ctgcgcatat atttcttcta aatgatcacg tccaaaatca
     3841 tcatatccgt atcctgttga tccttgtaga tcactttcgg ttgctttgac atgatgaaat
     3901 gcatttaata ctttttcttg attaatatat gctgtttctt caatttcttt aaaatatggt
     3961 gctaacgttg attcgacgtc agctactatc ttacttatat ctttcatttg aatttcgtcc
     4021 ttttattatt ttttaaatcc ttttatacga taaacatttt caatttcatc atatttaagt
     4081 tcagtcacta atgtatgttg ttttagaaaa tataatcgat ctgcattatt cgtcgcaatt
     4141 gtctcatcat aataagttaa ttgccttttg atttcatcaa taaataacgt cttaacaagt
     4201 aatttatcac catcattttt agaagaaaca aaaacattag gcaaatcact tgctggacaa
     4261 tttgatgcat gatcacataa gtccttttta ttaaaaataa ctatttgaga aatatgactc
     4321 atatctaatt gtttgattaa atcattaact gtgtcatact gcgtacggta ttcaggatgg
     4381 ctactatcta cgacatgtac taataaatct gcacctttag cctcttctaa agttgattta
     4441 aaagctgcaa ttaacgtcgt aggtagtttc tgtataaaac caacagtatc tgaaataatt
     4501 aaattaaatc catcatttat ttgaatttgt cgtgttttag gatctaacgt tgcaaataat
     4561 tgatcttttt catatgtctc ttcatttgct aaaacattaa accatgatga tttaccagca
     4621 tttgtataac caactaaagc tacttgaaac acctgatttt gatttctttt atttcgatat
     4681 ctttcgcgat gttcttctac cgtccgcaat tgatgtttaa tttcattcat acgagttcga
     4741 atatgtctgc gatccatctc taacttcgtt tcaccagggc ctctagttcc aataccgcca
     4801 cctaaacgag aaagactttt accatggcct tgcaatctag gtaataaata atcaagttgt
     4861 gctagctcta cttgcaattt accttcttta cttcttgctc ttaatgcaaa tatttcaaga
     4921 atcaactgag ttctatcaat aatttttaca cctaaagctt catttagtga tttggattgt
     4981 gcagtcgtta attcatcatt tgtgatgact acatcaatat ctttgaactc aataaatgct
     5041 tgaatttctt ccattttacc tttaccaaca taatatttgc gatctacacg atctctgttt
     5101 tgagtaattt gacccaacac ttcaagttgg caagtctctg ataaagatga taattcctcc
     5161 attgtagact caaaattaaa ttgcttatca tcttgagcat gtacaccgac taagacagct
     5221 ttttctagtt tatttttagt atcatgaatt tgttgctgag ccatgttata catctcctta
     5281 ataaaatcct actcaaagta ttctatcata gagttttagt aaagactatt acacgatttt
     5341 atggtacatt ttaagtaaat cgaaaggatg attcaatgga gacaatttat gattttgtag
     5401 tagaaacaaa taaaggtgtt acttacaaat tagatgcata taagggtgac gttatgctaa
     5461 ttgttaatac agctagcgaa tgtggcttta cttcacaatt tgaaggtttg caatcattat
     5521 acgagaaata caaagatcaa gggtttgtaa ttctaggttt cccttgtaat caatttggtg
     5581 gccaagaacc tggatcaggc gaagaagcag ctcaaaactg taaattaaac tatggtgtta
     5641 ccttccctat gcatcaaaaa atagacgtaa aaggggaaca tcaattacct ttatttagat
     5701 atttgactgc ggcacaacac ggattcttta atgagaaaat caaatggaat ttcactaagt
     5761 ttttagtgga tcgcgaaggt aacgttgtta aacgatttgc acctcaaaaa aaacctgttc
     5821 aaatcgaaag agaaatagaa aaattattat aaaatatact agctcatagc aaataaaacg
     5881 cttcatataa agtcgtatca tatattgact cgacctttat atgaagcgtt tttattacaa
     5941 acattcctct attgtatctt gtcatttgaa catcatttaa ttgatgtcac atatgacttt
     6001 atttctatat ctatttatca tacttcaaac gttatgtaca cgagtcatta aatcgtgccc
     6061 atatgattac aacttattct tcactttcag tagatgcttg accttcagtt tctactgtat
     6121 aagtgctgat cgcatgtttg taaatcaagt gttgtttgcc ttgagaattt aagcttacga
     6181 catacttgtc gtattcttca ataacacctt tcatttggaa accgtttaga aagaatacag
     6241 ttacttcagt ttggtttgct ttaaaattct ctagtgcttt gtcttggatg ttttcgtttg
     6301 caatcatctg tcggactcct tttacttaat ctgggttgta atctcatcta acatcatttg
     6361 aagtgacata ttttctttat ctaaccaatg aacactcatt ttattcttga accatgtcat
     6421 ttgtcgtttt gcatattggc gtgaatgttg ctttaaatca ttgacagcat cttcataaat
     6481 catttgtccg ttaatcacag gtattaattc tttatatcca atagcttgca tactttggca
     6541 agattcatag ccttgttcaa caagttgttg cacttctcta aataatccgt gatccaacat
     6601 aatatcaaca cgtttattta ttcttgaata taatgtttta cgcgacattt caatccctaa
     6661 taataatgta tcataatttt cagtaaattg ttgcactttc ttgcgattac tcaaaagttt
     6721 ttttgttttt aaataatatt caatagcgcg caacactctt tggcggttgt tagggtgaat
     6781 attttctgca gaaaccgcat caaattgagc taaataatcg tgtagttgct gattatctaa
     6841 atgttctaat gcagataact tttgtttaac tatggataat tgtgcaggtg taactgtttc
     6901 atcttctaat tcataattat atattaatga ttgaatatat aagcctgttc cacctgctat
     6961 gattggaact ttacctctat tcgttatatc agtaattaaa tcttctgcta atcgcttgaa
     7021 ttcatatgct gaaaatgtat catcaggatt caagatatca attaaatgat gtggaatacc
     7081 atccatttct tcaggtgtta cttttgcagt tccaatattc atatgtttgt aaacttgcat
     7141 agaatcaccg cttatgattt caccattgat acgcttcgcg agttctatgc taagctctgt
     7201 tttacctgaa gcagttggcc ccacaattac tacaataaaa ggcttatttt tattcatatt
     7261 taattccact ttctttattt tttataaaat tgattcaacc atttaaacaa gtcttcccaa
     7321 attttgtcat gatctttttc aaataacact tcatgacgtt tatgatgata aagattcact
     7381 ttaacatttt taatgccagc ttttctatat atattcgcca attttaaaat ccctttacca
     7441 taatcaccta aaggatcgtc atatcctgaa actaataata taggcatggc atgatttaac
     7501 attttcatat tttttaattg tgatgtatgt agcatataat aggctgtttg atataataat
     7561 tgatttgaca cattaaatcc actatatgga tctttaataa agttatctac ttcaattgga
     7621 ttactagaaa tccaatcact ttgtgtacgt aatggacgta tttttttatt aaaactattt
     7681 gatactaact ggttaaccca ttcaactcgt ttctcagcac cataaatttt tgtaatcagt
     7741 tgtaacactt taacggttgg taaacctttc cataatgaat acataccagt accacttaaa
     7801 attagaccat caacatattg cggatatgtt tctacaaaca atctagctat aacggatccc
     7861 attgaatgtc cgattataat gtatggttta tcaacattgc ctctcactgt ttgcgctact
     7921 tcaaaggcat caccgataac tcgtttcata tcatcgtaat gccctcttgt tgattcatca
     7981 atattaatac catgtcctcg atgattatga cgtatcacat cgaagccatg ctttgaaagt
     8041 gcatgtgcta atttatcgta acgttccata tgttcagcca taccatgaaa tatatgaata
     8101 attccttttg aatctacatc tgtaaaattc actttaactt caatattcgt attatcttgt
     8161 acagttaatt taaattgttt ttcagtcatc atctcacacc tttaataaca acgcttttat
     8221 attcgctttt attattatca tacatttagt gatgcatagt ttcaattgac taatgtatct
     8281 cgtataaaaa aactgatgcc cttaaaacag gacaccagtt tatgattgta caaattaatt
     8341 attctttaac tgctggttga ttatttccat gttgcgcttc tttaattgct tgctcaactt
     8401 cttcagtata tgcttcaatt tgagctggag agtaatcaag catatctgcc ataatatcga
     8461 tgacagcatc tttataatct aatacatctt taatattgaa atacatttta ccagaacgac
     8521 gaactaagaa atcgttaggt ttgtatacca tttcttgttg aatactataa acaagttcta
     8581 cataaatttc taatggtaac ttgctatcat ggtattgaga tgtttgcgca atgttgaaca
     8641 attcatcaac atttgaacca tatttagatg ctaaacgtct tgcaacatct tcatcaatgc
     8701 cgaatccttt agctacatct actttttgct ctacaaacgc atcaaagttc ttgctaccac
     8761 ctacatcgcc acctgaaatt gccagacctt ttgtattaca tggactaaat gttaaaccgt
     8821 agtctttttt caagcgttta gatactaaat caacaatgtc ttgagccatg tgacgatagc
     8881 ctgttaattt accacctgca atagttaata aacctgattt accttcccaa atttcatcct
     8941 tacgagagat ttcagaaggg tctttgcctt cttcgtaaat taatggtcta attcctgccc
     9001 atgttgattc aatatcttca tctgtaacat taacactagg gaacatgtaa ttaatcgcat
     9061 cgattaaata gtctctgtct tcttgtgtag ttaatggtga agatttgata ttgtcataga
     9121 atgtatctgt agtacctaca tacgcttttc cttcacgtgg aattgcaaaa atcattcttc
     9181 catctttttc agtatcaaag tatactgctt gacctaatgg gaattttgat tgatcaataa
     9241 caacatgtac acctttagtt aaacgtaatt ttttattatt gcgtgcataa tcaccacttc
     9301 taacatcatc aacccatgga cctgctgcat taaccacttt tttagcctta attgtataat
     9361 tttcattagt taatttatct ataactttaa caccatttac ttgttgattt ttatcataag
     9421 tgaagtgttc agatttagta tagttgataa tttctgcgcc tttttcagca gcacgcttca
     9481 taacttcaat agttaaacgc gcatcgtcag tacgatattc aacatagtaa ccgccgcctt
     9541 ttagaccttc ttttttaact aatggttctt tagctaaagt ttctttttta gataacattt
     9601 ttttacgttc agacttctta acacctgcta aacgatcata cattcctaaa ccaattgatg
     9661 ttgagaattt accaaatgtt ccacctttat gcattggtaa aagcatccac tctggagtcg
     9721 taacatgagg tccattttca taaacaatcg cacgttcttt accagtttcg gcaactactc
     9781 caatttggaa ttgttttaag taacgtaaac caccatggac taattttgta gatcttgagc
     9841 ttgttccttg tgcaaagtct tgcatttcaa ctaatgcaac tttcattcct ctttcactcg
     9901 cgtctagtgc aatacctgca cctgtaatac cgccaccaat aattactaaa tcatattcat
     9961 catttcttaa attcttttta atatgttctc tcttaaaagt agacaacgcc attccaaaac
    10021 gcctcctaaa aaatgtaaaa acgagagact tgtcccacat aaacaatcct tcaattgtac
    10081 atgttcgaac aaatctctcg cttctcgatt caaatattaa cttaaattga ttgtatcaca
    10141 atgtttatac aaaagtctaa tctacaagtt tattctgttt taaaaacttg tgttgcttca
    10201 acagcttttt tccaacctct atataatttt tctctttcgc cttcatccat tttcggatcg
    10261 aatttttctt ctaatttcca gtttttagcg atatcatctt tactctccca gaatccaact
    10321 gctaatcctg ctaaaaatgc agcacctaaa gctgtagttt cttgaatttc aggtctttca
    10381 acagaagtat taacaatgtc tgcttggaac tgcataataa agttgttttt aactgcacca
    10441 ccatcgacac gtaaactttg gacatcaatg ccagagtctt ttgacattgc ttccataacg
    10501 tcacgagttt ggtaacatag tgattctaaa gttgcacgga taaagtgctc tttttcagtt
    10561 ccacgtgata aaccgaaaat cgcaccacgt gcttcagaat cccaatatgg tgttcctaaa
    10621 cctacaaaag ctggaacaac ataaacaccc tcagtagagt caactcgtgt cgcataactt
    10681 tctgattgtg gtgctgaatt aatcattctt aatccatcac gtaaccattg gattgctgaa
    10741 cccgaaacaa agatggaacc ttcaagcgca taatttactt ttccatcaat accataagca
    10801 attgttgtta ataaaccact ttcagattta accgctttgt cacctgtatt cattaacatg
    10861 aagccaccag ttccatatgt gtttttcacg tcaccacgtt cgaagcaagc ttgtccaaat
    10921 aatgctgctt gttgatcacc agctacacca gcgattggta cttcttgacc atagaagtgg
    10981 taatcaattg tcttaccata tacttcactt gaaggtttaa cttctggcaa catattttta
    11041 ggtactgtaa gtagttctaa taactcatcg tcccattcta aatcatggat attaaacatt
    11101 aatgtacgac tcgcatttga ataatcagta atatgcgcag cttttcctga taatttccat
    11161 actaaccaag tatcaatcgt tccaaataat agatcgccat tttctgcttt ttctcgtgca
    11221 ccttcaacat tgtctagaat ccatttaact tttgtacctg caaaatacgg atctaaaagt
    11281 aatcctgtct tatctctaaa tgtttgttca tatccttgtt gttttaattc tgaacaaatt
    11341 gattgtgttt gacgtgattg ccaaacaatt gcgtgataaa ttgggcggcc agtatgtttg
    11401 tcccaaacca ccgttgtttc gcgttggttt gtaataccga tacctgcaat ttgatcagct
    11461 ctaacatcat tttcattaat tacttccgtc attacagcta acacagatgt ccaaatttca
    11521 tttgcatcat gttcaaccca acctgattgt ggaaaatatt gcttaaactc acgttgtgct
    11581 acccctgcaa tttccccttt ttgattgaat aaaatcgctc ttgagcttgt tgttccttgg
    11641 tctatagata aaatatattt ttccattagt agtatctcct ttattaaaaa ataatcgcta
    11701 acccattgta caaaatctgt taaattttgg cgaaaaaata gcataatcca tgaatcaaac
    11761 atgttaaatt atgctattta cgtttctttt tattttagta aattgattcg atatcacctt
    11821 tttttgatga tttatttaaa atgtaaccta aaatcaaagt aataataact acaacaattg
    11881 caattgcaca accaatatta aatgtatgtt tataaaatac agcgtatacc actgcaccta
    11941 ataaaccacc ggcaattggt cctaagatag gaacgattgc atatgaccaa tttgaaccac
    12001 ctttaccagc tattggtaaa atcgcatgtg caattctcgg acctaaatca cgtgctgggt
    12061 tgattgcata accagtagca ccgcctaaac ttaatccgat tgcaacaatt aatgctccga
    12121 caattaaagg atttaaacca tcggcaattt tgtttacacc gataaataaa atacctaaag
    12181 ttaatgccat tgttccgata atctcactta aaaagttggc aaagtaattc ttaatagccg
    12241 gtgctgtaga gaaaacacct aatttcgcgc cagcttcttc tgtcgctttc caatgtggca
    12301 agtacattaa ccatacaatt gttgctccga caattgcacc taacatttga gcaacaatat
    12361 aaccaggaac taatgaccaa tcaaaacttc cgtctaatgc aagagctaaa gacaccgctg
    12421 ggtttaaatg tgcacctgag aattgaccga cagcaaacac acccattgta accgctaatc
    12481 cccatccagc tgtgatgaca atccaatcag caccattcgc agcacttctc tttaaattga
    12541 cattggcaca aacgccacca ccaaaaagga ttaagattgc agttcctagg aattctgcta
    12601 aatatacatt catattaaat gtcctcccaa aaagacaaag agattcctac actagcagtt
    12661 tggctcgtag aaatctcccg tctctattct atgtattaac ttttattata tttgttatga
    12721 tactatcgat atgaaagcgt tgtcaatggt ttttgtaaaa attttgtcaa atttaatttt
    12781 tcaagtcttt ataaaagtag tttaatttga aaactagaaa tatcaatccg cactaattta
    12841 tttatgacgc cattatgttt aattgacatt cttcagccat attttttatg actagttgca
    12901 ttttttacta ataaacacct ctaaaacttt aatgatttta atcgttttag aggtgctaat
    12961 tatgttattt aattattctt tgtatgatac cttaaatacc ttattacata tcccatatga
    13021 aaacggcttt tttaatatgt tatatgacta aatctcgtga aaaatgaaat ttttgcagac
    13081 ttatgattta ccaaagttta tcataactag ttgttacata tttcgctcca tttttaacag
    13141 cttcattgac ttcatctatt gtattaatta ggccacctgc aatgacttgt gtgtttgttt
    13201 ctttctgaat atgatgaatc gctttactcg caacacctgg aagtacttca acaaaatcag
    13261 gttcaacttt ttttatcaaa tctatactgc gtttcaatgc ttgactatca ataataaata
    13321 ctctaaaaat cgttaaagta tttaatgatt tagctttttt tattacttta gatttagtcg
    13381 atacgatacc ttttggcttg tattgctgaa taataaattc acttgcaaat tcatcgtggc
    13441 ttaaaccttt tatcaaatct atatgaataa aacactctat atgattttgc ttcagcaatt
    13501 ccataatact ttttatatgt cctatatgca tatctagaag cacacacatt ttataatctg
    13561 ttttaatcag tttctctaaa tctttaatgt ttcttatggc aggcaatatg ttgttattca
    13621 cttgattcat cctctctaca tcacacgctt aaataatttt tctaattcgt attttgaaaa
    13681 attaatgata attggacgac catgtggaca tgtaaatgga tcttccgctt ctcttaattg
    13741 atcaattaaa tcagacattt catgtttttg taaataatga ttcgctttaa tagatttttt
    13801 acatgacatc atgattgcta catcttcacg taattttttg atatctactt ttttctcttc
    13861 caaaattagc tcaatcatat ctttaataat ttcttctact tcatctttag ggaaccaaac
    13921 tggatagcta cttacaatat aatcatgacc accaaaatgt tctaacatga tacctacttg
    13981 ttgaagctca tttttatatt gatcaatgac taattgttca tcttttgaaa aatgaaatgt
    14041 taacgggatt aataaatctt gtacttcatt ggtaacttca cctattttat ctcgaaaata
    14101 ttcatatttt attctttctt gagctgcatg ctggtcaatc atgtacatgc caaattcatt
    14161 ttgagcaata atatacgttc catgtacttg gccaacaatt tccatatatg gaattcttcg
    14221 tttagggtct ttactttttg ttcctttaat atcgtttaca tcataataat catcagacgc
    14281 tttattctca taattttgca cagatgtatc gtcattcgaa tcaaatgttt gatccatttc
    14341 ttgtagaatt tcttgttgct ttttgaagta gtcttcatct ttttcacgtg tattattata
    14401 accggaagat tcatcatcag ctattaaact ttcagacgtt ttcgttacga atggattata
    14461 tgaatcttct cttataacta tttcatcgtt ttgattttct accataaatg gcttactgtt
    14521 actttcttca gatgaaaacg tcttctcttg attattctct gtgttttgtc tttgttcaaa
    14581 ttcgattttt tgttgttcga atgaatgtaa cactttattt tttttcggca cataatccaa
    14641 gttattttta ggaattaata tacggtcttt aaatgcttct tgtattttgc tcactatcaa
    14701 ttgatatagt tgctcttctt ttgataaacg cacttctagt tttgttggat gaacatttac
    14761 gtctactaag attggatcca tttcaatatt aatataacaa atcgggaacc tacctattgt
    14821 taagagtgta tgatagcctt ctaaaatcgc tttatttagc ataaagtttt taatgtatcg
    14881 tccattaata aaaatagaaa tatagtgctt attacttcta gaatgttcag gctttgcaac
    14941 aaaaccttca atgtgataat cacttgtatc tccagatata tgtactaaat ctcgtgcaac
    15001 tttcatccca taaatctctg ccatcacttc attagttcgt cctgaaccat ttgtacttaa
    15061 cattgttttg ccatctgaaa tgagtgctat tcgaatgtcc ggatggctca ttgccattct
    15121 gttgacaata tcggttattt tacctagttc agtgtataaa cttttaatat attttaaacg
    15181 tgctggtgta ttataaaata atgattctac aagtatatct gttccttttt tcgcttttgc
    15241 aggcttatga tttaatattt caccattttc tacatatatt tcatttccat tagcattatc
    15301 cgtgcaagtc ttcaatgtta ctttagcaac tgatgaaata ctggctagtg cttcaccacg
    15361 gaatcctaat gtcctaatat gaaataaatc ttcatcttga tctaatttac tagtcgcatg
    15421 tctatgaaat actaatccta agtcttccgc ttcaattccg cttccattat cgactacgcg
    15481 aatagattgg acgccagatt cctctacttc aatgcttatt tctgtagcgc ctgcatctat
    15541 agcattttcc aacagttctt tcacaacaga actcggtctt tcaactactt cacctgctgc
    15601 gattttattt gctaatgagg tttggagttc tttaattttc cccattttgc aacacctcta
    15661 ttttaattga ttttgtaatt cacttaactt caccaatgcc tcaattggtg tcatattaga
    15721 taaattcaaa tttttaattt gtagttcaat ctcgctttct tgatcatttt caaacaaatc
    15781 aaatgatgct tgttcaaagt ctttttgaga taaagtatca gttgtttctt caacacttaa
    15841 gtttaaattt tcttgattaa tttcaggttc attttcgacc atttttaaat ttgatatcga
    15901 tgatttttta ccagcagacg cttcaaactc gcttagaatc acttgtgctc tgctaataac
    15961 tttttcaggt aaatcagcta atttcgcaac ttgaatacca taactatcgt caactgcacc
    16021 atctttgact ttatgcaaga atataagttc acctttatat tcattagcag cgacgtgaac
    16081 attttttaga cttggtaatg cttgatctaa tgttgtcaat tcatgataat gtgttgaaaa
    16141 taacgtttta gcatgtgatg tttcagctac atactctatc attgcctgcg ctaaagctaa
    16201 accgtcatac gttgaagtac cacgtccaat ttcatcgaaa ataatcaaac tatcctctgt
    16261 tgcataagtt aatgcctttt gtgcttctag catttctacc ataaacgtac tcttacctga
    16321 aaccaaatca tctgccgcac ctattctagt gaatatttga tcaaatatag gtaacactgc
    16381 ctctttacaa gggacataag ctcccatttg ggccattata ctaattatgg caacttgtct
    16441 catatatgtc gatttaccag acatattcgg acctgtaatt aaatatataa atgtttcatt
    16501 atctaatcga caattattag gcacatagtc attataatcc attactcttt ccactactgg
    16561 gtgcctagat tccactaatt ctaatgtttt attttcacta aatgaaggcc tagtgtaatt
    16621 atatttttga gcaatttctg caaagctctg taaacaatct agctctgaaa taattttagc
    16681 ttgttgttgt aaacgttcag tatatttttt aacttcttca cgtagctgaa caaataattg
    16741 atattctaat tcgatggctt tgtcttccgc acctaaaatg atatcttctt tttctttaag
    16801 ttcatcagtt ataaaacgtt cagcattcga taacgtttgc ttcctcatat aaccaaattc
    16861 acttggttca aaattttgca agttggcacg tgttatttct ataaaataac caaacacttt
    16921 attaaagctt attttcaatg attttattcc tgtacgttgt ctttctttgg cttgtaattc
    16981 tgctaaccat gtttttccgt tttttgaagc ttcaagatat tcatctaatt gcgtattaaa
    17041 accaactttg aatagtccgc catctttaac tgaaattggt ggttcttcta ctaaactctg
    17101 ttctaatata tcaagtaaat catcaagggg ttctagttga ttaacttgta caagcgtatt
    17161 ctgattcata gaatttagta atgctttaat attcggtatt tcagaaatgg aatgtttaag
    17221 ttgaattaaa tctctcgcat taacatttcc gtaactaaca cgcccaacaa gacgttcaat
    17281 atcatacact tgattaagat atgttcttaa ggtgtctctt tctatgaaat gagcactaaa
    17341 ttcatcaacg atatctaatc gtgcttcaat ttgttcttta cttattagtg gtctatctat
    17401 ccattgtttt aagcggcgtg ctcccattgg tgttttcgtt tcgtccatta gccaaagtag
    17461 cgttcctttt tttgatttta atcgaatgct ttccgttaac tcaaggtttc tcttagcata
    17521 aaaatccatt ttcatataat ctatagctgc atattgaaca acatcctcga tatgcgataa
    17581 atcacgtttt tgtgtatgat gaatataatc tagcaataat tgtgtcgctt gatacattaa
    17641 tttatgttca gtttgattca cactatagat ttctgatgat aacgtttccc tgactgttat
    17701 tgtttctgtc accatattaa tttgtctttt taaattatcg gaaatattgt cattgataac
    17761 aacttcgtta gggtttatcg tcgtaatttc atttaataaa gtcgcttcat cattaaaatg
    17821 tgtaaccttt aattcgccag tagaaacatc acagtaacta agcgcaattt caggttgatt
    17881 cataacaaaa cttaaaatat agttattttg tttatcatct acaccacctt gctccatcac
    17941 agttcctgga gtcacaattc ttactacctc acgtctaacc atacctttcg tttgtttcgg
    18001 atcttccatc tgttcacaaa tagctacttt atatccatta ttaacaagtg tatctatata
    18061 actatctgca gaatgatacg gaacaccaca catcggaatt ggattttctt ttttagcatc
    18121 tcttttagtt aaagtaattt caagtacacg tgatgcctcc ttggcatctt cataaaacat
    18181 ttcatagaaa tcacctagtc taaaaaataa taagcaatct tggtattctg attttatttt
    18241 taaatattgc tgcatcattg gtgtaacatt agacatatta tttcttcaca acccttgtct
    18301 ctttttaaaa tttgtcttta caatatattc gtttgtaagc tttttaatta ttaattattt
    18361 aacttataca ttttaacata cttactttta caaacctatt cataccatat aatcacgaag
    18421 catcttaaat gtataagaaa acgcctcaaa cctaataaaa tgtgtcaata gcatgtttag
    18481 aattaaatta aaattctaac attcaagaca tttaattaag taagggcgtt caatattaaa
    18541 atgaacaatg actctgtttg aaatcatata tcataaaatt attttataaa cctttgaaga
    18601 ataccacgtt tttttagagt aattaataag aaataactta taatagatcc gatagcactt
    18661 gagactatga acgtaatcat taacggttta atgaagaagt cttgaagccc aaggaaatat
    18721 gctaatggta tacaaattaa acttccgatg acaccagttc caagtacttc accgaccgcg
    18781 gccatgaata tatgtttacg atatatgtaa aacatactag ccaataaaac tccaatcata
    18841 ctacctggaa atgcaaaagc tgtaccagta ccaaaaagaa ctcttaaaat tgatgatata
    18901 agcgcttgag ctaatccata ccaaggacct actatgaccg cacttaatac atttacaaaa
    18961 tgctgtactg gtgctgcctt aactggtcct agaggaatga tgataatact gcttaataca
    19021 acatttattg caattaaaag tgcagttata gccagttttc ttgatttcat atgattgttc
    19081 tcctttttgt ttgtaattaa tcactatgct tggctttatt atggtcattt aaacgtgttt
    19141 ccattgttga tacaaacatt ttcaataatt gattcgcttc atattgtgaa gtttgaaact
    19201 gttcaactat gggcaatgta tttatttctg cttctatact ttgaatggta tgttccgact
    19261 gttctagcgc attttgtttc ccgtaatttt gaaagtttac tgcttgtttt tgatgctttt
    19321 ttaacatatc cattttagtc tttatcgttt ggttctgatg aatctgtgct tcaatttgtt
    19381 gatatgtttt gatagtatcc aaatttttaa ttttatttgc aatattatcc gcttgtttca
    19441 acacgtcatc tttattatac atttattgaa tcaccatttc cggctctact tcctttacaa
    19501 aactgccatt taatgaatac tgtttagctt catctattcg tacttccact agtttaccaa
    19561 tcatttcttt aggcgctttg aaattaacta gcttattttt atcagtgtag ccagcaagaa
    19621 cctgatcatc ttttttacta ctaccttcac aaagtactgt tacagtttgt ccttcgtact
    19681 tactcatagc tatttgtgaa taatgaccaa cttttttatt caaacgttgc aatcgttcct
    19741 ttttgacatt taaaggtaca ttatctttca ttttagcagc aggcgtacca tcacgttgtg
    19801 aatacaaata cgtatatgca tgttcaaaac caacttcatc atacagagtt aaagtttctt
    19861 caaattgttc ctctgattca tttggatacc ctacaataat atctgtagtt aatgctacat
    19921 taggaattct atctttgatt cgttttacta aatccaaata actttctcgt gtatattttc
    19981 tacccattat ttttaatact gcattatttc cagattgaac tggcaagtgg atatgaggaa
    20041 cgatattacc accctctgaa ataacatcaa tcatgtgatc tgtaaagtcc caaggatgac
    20101 ttgttgtgaa acgaactctt ggaatcgcta ttttagaaat tgcttgtaaa agatctccta
    20161 agtcatattc tatatcctgt aaatctttac cataagaatt tacattttga cctaaaagcg
    20221 ttatttcttt gtaaccttca cgagcaagtt cacgtacttc atctataatg tcttcaggtc
    20281 tgcggcttcg ttctttacct cttgtaaatg gaacaatgca atatgtacaa aacttatcac
    20341 aaccatacat aatattgacc catgctttaa tgttgccttc acggactttt ggaagatttt
    20401 caataacgtc tccttcttta gaccatactt caacaaccat tgctttagat aagtatgctt
    20461 cttctaaaat ttctggtaaa tgatgaatat tatgtgtacc aaatatcata tctacatttt
    20521 gatacgattt taaaatttta ttcactactg actcttcttg tgacatacaa ccacaaacac
    20581 cgattaaaat atcaggtcgt tcttttttca aatgcttcaa attacctatt tcactaaaca
    20641 ctttgttctc ggcattttct ctaatcgcac atgtattaat taaaataaca tctgcagtgt
    20701 taatatcagt cgttgcttga tagcctaatg cttcaagtat accagcaatg acctcagtgt
    20761 catgtgcatt catttgacat ccatatgttt taattaaaaa tgtacgctcg ttccccatac
    20821 cgcgatattt ttcatcaatt tgaaaatctc tattataacg aacttcttgt ttacctcttt
    20881 tttttgcttc ttttaaatta ggcggctgat aaacatgttc aaaatattta ctataatctt
    20941 tttctgcttt cttatctctc tcagctaaaa catctacaga acttgctttt ctttgttctt
    21001 cgttcacttt acaaaatcct ttctatttgc ccgtatcatt taataattat attaaattta
    21061 actataaagt gcaaaaacag ttaaagttat acgtcatctc taacaagcca attcaaatcc
    21121 agtttacttc acttttaatg ttattcatca tatttttcag ttaaagtttc aatagtgatg
    21181 ccatctggca catgataaaa cttaacttgg ctaataatac ttggacattc aagttccacc
    21241 attcgttcat ttaatgattg tattaaaatt aaacattcat tcatatttcc ttgtactgtc
    21301 gtttctaacg gacctactct aaaatgcata ccagattcgt caattatttt aatcgcctcg
    21361 tctacgtaag gtataacatt gtcattgttt ggtgttttag gaattatttg tatactcatt
    21421 aatgtgtctt tcatttttaa aaactccttt attttttgta atagtaaaaa actgagtagc
    21481 ataaatggat ctgttataaa tacatttata cgactcagtt ttaattatta aataaattgt
    21541 tcagttaatg cattgaaggt gtcttcagta attttgatat ctcttttagc aagtggtgta
    21601 tcatctaact ctttaatttg agattcatat gatggtgttt ctttatcttg ataaacaata
    21661 ccagttacta aagattcatg ttcaataaca gttttagtcg ctaattgttt atctgtagaa
    21721 tcataatttt caatgtcatc aacacttgtt aaatgttctt taaaccaatc gtatgtgtta
    21781 attttattat aagtcacaca tggtgaaaag acattaacga atgaaaatcc atcatgatta
    21841 attgcatctt caattagttt tgttaatcct ttaatatcgc ttgaaaaacc ttgggctaca
    21901 aatgtggcac cagatgataa tgctaattct aaaggcgcaa catttttttc tatattacct
    21961 tttggcgttg ttttagtaac aaatcctact gctgatgacg gcgatgtttg tccctttgtc
    22021 aaaccataaa tttgattatc catgactata tacgtcatgt tcatatttct tcttaaagca
    22081 tggattgtat gccccatacc tatagcataa ccatcaccat ctcctccaga tgcaataaca
    22141 gttaaatctt tattcgccat ttttacacct tgagctaaag gtaatgcacg tccgtgaata
    22201 gaatgaacgc cataagaatt aatatatcct gaaagacggc cagaacatcc tataccggtg
    22261 atgatagcta cttcttcagg ttctaaccct atatttgcgg ctgctttttg aattgcagct
    22321 tgtactgaga aatcgccaca tccagggcac cagttaggct taacattatt tctaaaatct
    22381 ttaaatgtcg ccatctatac catctccttt atttcagtag caattatttt gccttttact
    22441 tcgatttcat gtggtaggaa aggtgtccca tcatactttg tataattttc aatcttatca
    22501 tgaatattga cattcatttt tataatacta gccaattgtc cttgataatt gtgctccact
    22561 acaacgactt tcttcgcttt attaactgca tcttgaataa cgcttgttgg gaatggatgc
    22621 aattgtctaa tttgtatagt gttaactttt atgccttgtt gattcaaacg gttactacct
    22681 tcttgaattg caccttttgt agaaataaaa ccgatataaa gaatatctgc atcttcatgt
    22741 tgtaagttag cttctactgg cgattcaatt agtaactgct caattttacg cattcgtttt
    22801 tccatttgtt gttgtctatt tgatgcagat tcactaggtt taccttcttc attgtgttcc
    22861 acaccagtta tatgatgaat acctccttta ataccgggga taggtctagg agaaacacca
    22921 ttggatgtta acgcataacg cttgaaataa cctttatcat cttcttcacg ttcaatatca
    22981 gattgaatga tttcaccacg tttaatttca atgcgattat aatctaattt ttcaacagtt
    23041 tgtttaccta aagataattg caaatcactt aaaactataa cagggcattg atattgttct
    23101 gctaaattaa aggcttccat agttaaataa aatgcatctt ctgcatcagt tggtgctaca
    23161 actatttttg gaatatcacc atgtgtacca taaatcattt gcattaaatc tgactgttct
    23221 tgtttcgtag gtaatccagt agaaggtcca cctcgttgag tattaataat gactaatggc
    23281 gtttcagtca taccagataa tccaattgct tccatcatta aagataaacc tggaccagca
    23341 ctagccgtaa acgctctgac tccaccataa tttgcaccaa tagccatagt tacagcagca
    23401 atttcatctt ctgtttgaat aaccgctccg tttactttag atatattggc aatcatatat
    23461 tccataactt cagacgcagg tgtaatagga tatgccgcca taaattgtga acctgcagca
    23521 attgcaccta atccaatggc atcgttacca atcatatata gatgtggtag tgcatctgtt
    23581 gactctaatt caaagtcccc gtcgatttca ggtaagcggg attgcattaa ttgataacct
    23641 tcgtttaatg cttggatatt gacttcaact accttgtcac cttttttaga aaacatatta
    23701 gtaataagtt cttcaaatgt atttgtattc aaattcatca atgcgctagt agcacctatt
    23761 gcaaccatgt ttttcattaa tgctgtacct aattctttag cggttgctgt aaaaggtaat
    23821 tcaataagct gtgcatgaca tccttctggt ttcacaggtt tagccttggc atcagctaaa
    23881 ataatactgt cttctctcat ttcatgatgg ttaacatcaa ttgtttcttg gtcaaatgca
    23941 atcaaaatat ctaaatcatc actaattgca tgaacaggcg tcgtagaaac tctaatttta
    24001 ttattcgtat gtccaccttt gatacgactt gaaaaatgtc tatatccata taaataatat
    24061 ccttttctat tcatagccgt agcgaagatt tccccagttg attcaatacc ttcgccttgt
    24121 tgaccgccaa ctttccacga taattgtggt ttcatagtta tatgcctcct atggatatat
    24181 ttcatattaa atgataacaa aaagaaccac cgatttacta tgtaaacagt ggtactatat
    24241 taaattcata ctaatggacg ataggtccaa ttcgaatgtt tacttacgta attttaaaat
    24301 gttgaaaatg gatggtcatc atttatcaat atacgttcga tgtgctttgt tttaccttct
    24361 ttgtctaaat caataacaac accagataat acacttctac cttcatttgg aacaacatgt
    24421 ctttgtggca aactagtgat aaaacgctca attacctctg ttttatttat tcctaaaatg
    24481 ccgtcataaa aacctgtcat accaacatcc gttatatacc ctgtcccctt tggtaaaata
    24541 cgttcatctg ctgtttgaat gtgtgtatgc gttccaacaa cagcgctagc tctaccatct
    24601 aaatgccatc ccattgcata cttttcagaa gttgtttctg catgaaaatc aacaaatata
    24661 aacggagttt gttcttgtgc ttccttgact aattgatctg cctttttaaa aggatcatca
    24721 atatctggca taaacgctct tccttgcaga ttaataactg caagtttaat atcattaatt
    24781 tgtataaatc tcataccaat tcccggcgct tcatccggaa aattcgctgg tcttactaga
    24841 cgttttgctt catctataaa atcataaatt tcacgttgac catatgtgtg attacccata
    24901 gtcatgaaat ctacaccatt tcttagtaat tgtttatata ttttttcagt caaaccttta
    24961 ccatgtgctg cattttcagc atttacaatt gtaactgttg gtttatactt ttgcttcagt
    25021 tgaggtatgt acgtttcaat tgcgtctcgt ccaattttac ctacgatatc ccctataaac
    25081 attattctca tgttttgttc atcctctcat aaatactagt ttaattcata cgttctaata
    25141 tttaaagtaa atattaaatt ctttgaatta tttttcttca attataaaga gacattactt
    25201 tgtctgaaaa cttcaatatg ttagtattaa gacatcaaaa taaataggag ttgtaaataa
    25261 atacatgtct attaatattg atccagaaaa atttgcagaa ttagtggtta tgtctaaccc
    25321 atcaaaattc gaagatgcag aggatatagc taaagaaagt ttaaagcttt acattaatgc
    25381 atatcgttta gcagagagat attcaacaat tgcaacaaat tgttatgata ctgcagaagt
    25441 tataaaagaa ttaaagaaaa cagatttaca attaaaataa ctcaacttag aaataaatcc
    25501 taattaatat tgcagaagag aagatgtgtt acatc
//
LOCUS       DBNENW010000020        25525 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNENW010000020 DBNENW010000000
VERSION     DBNENW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.020"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..360
                     /gene="lpl8"
                     /locus_tag="AC1LW8_000673"
     CDS             <1..360
                     /gene="lpl8"
                     /locus_tag="AC1LW8_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HIP4772538.1"
                     /translation="EDPKLKKRIENFKFFGQYANLKELKNYNNGDVSINENVPSYDAK
                     FKMSNKDENVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKGEKSDLS
                     VIDSLNFQPAKVDEDER"
     gene            350..1675
                     /locus_tag="AC1LW8_000674"
     CDS             350..1675
                     /locus_tag="AC1LW8_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772539.1"
                     /translation="MKDKYKIDPGPIKYNTEETTAISKISYEIENAKLYGIKSKTIGR
                     QIDQLKEAKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNFTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKHSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            1672..1995
                     /locus_tag="AC1LW8_000675"
     CDS             1672..1995
                     /locus_tag="AC1LW8_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772540.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2014..2328
                     /locus_tag="AC1LW8_000676"
     CDS             2014..2328
                     /locus_tag="AC1LW8_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772541.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRSVVAPEHK
                     HKYKDIENGLKGEEKVLIEQMAHHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(2612..2872)
                     /locus_tag="AC1LW8_000677"
     CDS             complement(2612..2872)
                     /locus_tag="AC1LW8_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772542.1"
                     /translation="MSTLLYNGDKDISHERESRCINMLFVILVLYIIGITFILLGVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3004..4206
                     /locus_tag="AC1LW8_000678"
     CDS             3004..4206
                     /locus_tag="AC1LW8_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIP4772543.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTINSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(4863..4925)
                     /locus_tag="AC1LW8_000679"
     CDS             complement(4863..4925)
                     /locus_tag="AC1LW8_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HIP4772544.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(4989..5057)
                     /locus_tag="AC1LW8_000680"
     CDS             complement(4989..5057)
                     /locus_tag="AC1LW8_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HIP4772545.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5109..5174)
                     /locus_tag="AC1LW8_000681"
     CDS             complement(5109..5174)
                     /locus_tag="AC1LW8_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HIP4772546.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5206..5271)
                     /locus_tag="AC1LW8_000682"
     CDS             complement(5206..5271)
                     /locus_tag="AC1LW8_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HIP4772547.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            5791..7275
                     /locus_tag="AC1LW8_000683"
     CDS             5791..7275
                     /locus_tag="AC1LW8_000683"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HIP4772548.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHMSNSTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7288..9993
                     /locus_tag="AC1LW8_000684"
     CDS             7288..9993
                     /locus_tag="AC1LW8_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HIP4772549.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMADSYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKITQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNDHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10155..10517
                     /locus_tag="AC1LW8_000685"
     CDS             10155..10517
                     /locus_tag="AC1LW8_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HIP4772550.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(10746..11162)
                     /locus_tag="AC1LW8_000686"
     CDS             complement(10746..11162)
                     /locus_tag="AC1LW8_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772551.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11200..11874
                     /locus_tag="AC1LW8_000687"
     CDS             11200..11874
                     /locus_tag="AC1LW8_000687"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIP4772552.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            11911..12645
                     /locus_tag="AC1LW8_000688"
     CDS             11911..12645
                     /locus_tag="AC1LW8_000688"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4772553.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSDILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            12823..12936
                     /locus_tag="AC1LW8_000689"
     CDS             12823..12936
                     /locus_tag="AC1LW8_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772554.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPGILM"
     gene            13101..14438
                     /locus_tag="AC1LW8_000690"
     CDS             13101..14438
                     /locus_tag="AC1LW8_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIP4772555.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            14655..15560
                     /locus_tag="AC1LW8_000691"
     CDS             14655..15560
                     /locus_tag="AC1LW8_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIP4772556.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            15553..16695
                     /locus_tag="AC1LW8_000692"
     CDS             15553..16695
                     /locus_tag="AC1LW8_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HIP4772557.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVITALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            16992..18017
                     /locus_tag="AC1LW8_000693"
     CDS             16992..18017
                     /locus_tag="AC1LW8_000693"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIP4772558.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPEELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            18021..18680
                     /locus_tag="AC1LW8_000694"
     CDS             18021..18680
                     /locus_tag="AC1LW8_000694"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIP4772559.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            18717..19559
                     /gene="gmpC"
                     /locus_tag="AC1LW8_000695"
     CDS             18717..19559
                     /gene="gmpC"
                     /locus_tag="AC1LW8_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HIP4772560.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     AITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            19891..20895
                     /gene="aaa"
                     /locus_tag="AC1LW8_000696"
     CDS             19891..20895
                     /gene="aaa"
                     /locus_tag="AC1LW8_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HIP4772561.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21080..21349)
                     /locus_tag="AC1LW8_000697"
     CDS             complement(21080..21349)
                     /locus_tag="AC1LW8_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772562.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            21498..21893
                     /locus_tag="AC1LW8_000698"
     CDS             21498..21893
                     /locus_tag="AC1LW8_000698"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP4772563.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESRVQALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            21883..22368
                     /locus_tag="AC1LW8_000699"
     CDS             21883..22368
                     /locus_tag="AC1LW8_000699"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4772564.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(22537..23319)
                     /locus_tag="AC1LW8_000700"
     CDS             complement(22537..23319)
                     /locus_tag="AC1LW8_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIP4772565.1"
                     /translation="MNAVFILALLLLFLMIIFGGRKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(23316..24434)
                     /locus_tag="AC1LW8_000701"
     CDS             complement(23316..24434)
                     /locus_tag="AC1LW8_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIP4772566.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKNRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(24549..25433)
                     /gene="gltC"
                     /locus_tag="AC1LW8_000702"
     CDS             complement(24549..25433)
                     /gene="gltC"
                     /locus_tag="AC1LW8_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:1902065 - response to L-glutamate [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate biosynthesis transcriptional regulator
                     GltC"
                     /protein_id="HIP4772567.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
BASE COUNT         8644 a         3772 c         4999 g         8110 t
ORIGIN      
        1 gaagatccaa aactaaaaaa gagaatagaa aactttaaat ttttcggaca atatgcaaac
       61 cttaaagaat tgaaaaatta caacaatggt gatgtctcaa ttaatgagaa tgttccaagt
      121 tatgacgcaa aatttaaaat gagcaataaa gatgaaaatg ttaagcaatt aagaagtcgt
      181 tataatattc ctactgataa agcaccggta ttaaaaatgc atattgatgg taatttgaaa
      241 ggaagttctg tgggttataa aaagttggaa attgactttt caaaaggtga aaaaagcgat
      301 ttgtcagtaa tagattcatt aaatttccag ccggcgaagg tggatgaaga tgaaagataa
      361 atacaaaata gatccaggac ctattaaata taatactgag gaaactacag ctatttctaa
      421 aataagttat gaaattgaaa atgcgaaatt atatggaatt aaaagtaaaa caataggtag
      481 acaaatagat caattaaaag aagcgaaaaa attcccttct aatcttgaat atgttgatag
      541 ttatactgat tctcttaatg gagtaacaac ttctgctttt ttaaataaag atacaggcaa
      601 agtaactctt gggatgactg ggactaatat acaaggcgaa gcctttaaaa agttaaaaga
      661 aggtgaattt tcaagacaaa attttaccaa tgctttggaa acagttaaag atggatatgc
      721 agatcttaaa atattatatt ctcctgcatc tgatcaaaac tatagatatg cgaatacgca
      781 agaatttata aataaaataa aaagtaagta tgatattgat tttattactg gacattcact
      841 aggtggaaga gatgcggtag ttctaggaat gagtaatggt attccgaaca ttgtggttta
      901 taatccagct cctatttcta taactagttt gaatcctaat tccccagatg gaaaacgatt
      961 gttagagtta tataaaaatt ataaaggtaa tattactagg tttgttgcag aaaatgatgc
     1021 attgacagaa aatctgaaga aatataagca ttatgttttt ttcggtaatg ataaagtctt
     1081 taaaaatggt aaaggtcatg aaatggaagg ctttctgacc gaagaagaac aaaaagctat
     1141 aaaaaaagaa cttaaaaaac tacaaggtta tgcagaagaa aataataagt catttgtaaa
     1201 gcattcaaat aatgctatct ctaaattagc tagtatagaa ttacttagag caaatatgat
     1261 gactacaaat ggaggaggat tatcttcttc gcagcaaaaa gttttagaaa gtttaacagc
     1321 tttaacaatt gcgcagtcat tcagtcaact gatagacgat gaaattaatc aaatcaaaaa
     1381 aatgtataat gaaaagaaaa agaaatttgg aaaaaattgg gaagacgctc aaaaagctgg
     1441 aaaagctgta ggtgaagatt tgagtgtaaa tggagttctc aatgctttag atgaaggtca
     1501 agtgaatgaa agtagtatgg taagagaacc tgaacaaatg atatctgcaa aagaaagaca
     1561 actttcaacg atagggtctt ctgtatcaaa ttatattatg agagttagac tcagtattaa
     1621 tgaaatcgtt gataaagatc aagtgcttgc atcacaaata ggtggattac tatgatgttt
     1681 aatcaaatta ataataaaaa tgaattagaa gaatcatatg aatctgagaa aaaacgtata
     1741 gagaatgaac tgcaaaattt aaatgaactt aggcatagaa ctcgaaaaga aaatgaacgt
     1801 agttatgatg tttttcaata tttgaaacac gaaatgaatt atagtgaaga tgcacaaagg
     1861 aaaatgacga gaaatataga agcgtatgag caagaaatca atgagataat tagaaagcaa
     1921 gaatggaaat tagaagaata taaagaagac ttaaaaaagt cttatgaaaa gcagttagat
     1981 aaactaagtg actgataatt tggaggagat taaatgactc taatagaacc agatatgacc
     2041 ttaagaatgc cagatataag tactacagta gaaacactta atctcatatc taaaatgaat
     2101 gctcaaaaag aaaacattcg ttcagttgtt gcacctgaac ataagcataa atataaagat
     2161 attgaaaatg gattaaaagg tgaagaaaaa gtattaattg aacaaatggc gcatcattgc
     2221 gaggctttta aagctaattt taaaggcgcg gctcaaggag attgggttaa aagtgccatg
     2281 tctgagatag acagcattaa ggatgacctg aaaaaaatta atagctaaac tacatatcgt
     2341 actactaaat aaagcgacca atgttcagtg tattcacaac tgacactagg ccgcttcttt
     2401 ttattttatt cccatgtgaa gtaattcgaa ctgaatcgaa atatcacact tacaattgta
     2461 aaaacacaac ttaaattact gtcccgcatt aaacatatcc aaattccaaa cagcaacaac
     2521 aaaatcctca agtaccaaaa gctatgacct ctttatcaaa atcaacgcaa gtcaaactct
     2581 ctcgaactat ccgccataaa tcatcctcac cttacaaacc ataaagcgct gcagttaagt
     2641 attgaatggc atagccaatt gaaatgaaaa tagcaatcgt tataatagca ctgccaatgg
     2701 tatataaagt atgtttcgta gataatcctt cagtctttga accaaaaaca ccgagtagaa
     2761 taaatgtaat accaatgata tataaaacta aaatgacaaa tagcatgttt atacacctcg
     2821 attctctctc gtgagatata tctttgtctc cattataaag taaagtgctc aataatttaa
     2881 ttaagtatac tattttttta ttatcgaaga ttgcgaaaga ggaaatgctc ctatataatg
     2941 taaaacggaa ttattcctat ttgcaaacga acgaaacaaa taaattgaag tgagggtata
     3001 tatatggcta aaattccagt tacggtatta agtggttatt taggctcggg gaaaacaacg
     3061 ttgttaaatc atattttaca aaatcgagaa ggtcgacgta ttgcggtaat tgtaaatgat
     3121 atgagtgaag tcaatatcga taaagatctt gtcgcagatg gtgggggact atcgcgcaca
     3181 gacgaaaaat tagtcgaact ttctaatggt tgtatctgtt gtacacttag agacgattta
     3241 ttaaaagaag ttgagcgttt agtgaaaaaa ggtggcatcg atcaaattgt tattgagtca
     3301 acagggattt cagagccagt acctgttgca caaactttct catatattga tgatgaactt
     3361 ggcattgatc ttacagcgat ttgccgttta gatacaatgg ttacagttgt ggatgctaac
     3421 cgcttcgtac atgacatcaa ctcagaagat ttattgatgg atcgtgatca aagcgttgat
     3481 gaaacagatg agcgttcgat tgctgattta ttaattgacc aagttgaatt ttgtgatgta
     3541 ttgattatta ataaaattga tttaattagt gaagaagaac ttgcgaagtt agaaaaagtg
     3601 ttaagtgcat tgcaacctac tgctaaaatt attaagacaa taaattctga agtggattta
     3661 aaagaagtct taaatacgca gcgttttgat tttgaaaaag cgagcgagtc agcaggatgg
     3721 atcaaagaac ttgagtctgg tgggcatgca tcgcatacac ctgaaacaga agaatatggt
     3781 atatcatcgt ttgtatataa acgtcgactt cctttccatg ctaaaaggtt caatgattgg
     3841 ttagaaacca tgccaaataa tgtcgttcga tcaaaaggta tcgtatggct agcacaatac
     3901 aatcacgtag catgtttatt atctcaagca gggtcatctt gcaatattca tccagttaca
     3961 tattgggtgg ctagtatgtc tgaagcacaa caaacgcaaa tattagcaga acgccaagac
     4021 gtcgcagctg aatgggatcc agaatatggc gatcgtcata cacaatttgt cattattggt
     4081 accgacttag atgaagaaaa attaacaaaa gaactcgatg catgcttagt caatgcgcaa
     4141 gaaattgatg cagattggca acaatttgaa gatccatatc aatggcaaat tagaccagca
     4201 cgataagttg aatgaagtat aatcattttt aaattgtggc ttaattgttt gcaatttatg
     4261 acaattaata aaaagttaag atgttcttag gatctttttg ttttcaatct atctaaagtg
     4321 tgatagtttt gataaagcag aaatttgcat tttatccata gggctaggac atgtatatgt
     4381 cttagtcctt tttatgtttg ctttgatgaa aaatggcaaa accatcgtac caactgttaa
     4441 ttgaaggtac aatcattgtt taacagtcat aaaaatgagt gaagtaaatg cagctatcaa
     4501 tgtactatta acacttacgg tcacacgagt gtttcagtct aaacgtgcaa cgcaattctg
     4561 cacgtggcca atatatcttg ggatagatta tgaaaatcat taagatttcg tatgccaaat
     4621 catgaatgtc tcagacctcg agtatcaaac ataaaacacg ccacaaaaat ataacgcgtc
     4681 agactacaaa gatcacgaat attccgtacg tcccaccaag aacatcctat acaaaaaacc
     4741 aagaccattt atttgcaaca aactaacaag actcacctca catcaataaa tcaacacaaa
     4801 gcaaagccac catccctatt ggtatagtgg cctgagaatt ttaagtattc aattcgctta
     4861 aattattttg cgaaaatgtc gataattgct ttgatgattt taatgatagt acctacaata
     4921 gccatcgttt tgtcctcctg tatgttgatt taacatagtg atgagttgtt gatcgttaat
     4981 gtttgagatt agttgttacc taaaaattta ccaagtaaat ctttaaagaa tttgaataat
     5041 tttgctacga attccatgtg aatggccccc ttcaaataag atgttcatat ctatgagatt
     5101 tttataactt acttaccagt gaatttctca attaatcctt taatgaattt aatgattcct
     5161 gcaatgatac ccatgtgaaa gacctccttt gtttgttatg aaatcttatt taccagtgaa
     5221 ttgttcgatt aagcttttga taactttaat gatgccagcg atgataccca ttaagattac
     5281 ctcctttgct tatgagttaa cttcattgta catagttatc ttgtgcgtaa ttgatttttt
     5341 tcctaacttg aattgttatg atcataactg cgaatttttt attttaaaag gacgggaaat
     5401 actgcctaac tgtacaggtg catttacaaa atctttacaa tcattggttt aatagaggtt
     5461 aagctcatga atttgacatg aagcagaaat ttaattatat tttatgttat aagtttaaaa
     5521 cgaataacac gttaggtctc gtctaggcaa agcatattta tgaagatgaa ttgaacagac
     5581 agaagtaaca ttttatatac atatgtgcat gggagcacgc ttaaagagaa caatgcaaca
     5641 aggtatatca acgtaccgtg atgcagtgtc tagttaaact gaaacctgca atagtaaatt
     5701 ttctgccatt atgtacagaa tctactattg taggttttta ttttatacat aagcaattat
     5761 gcgagagatt agaaataagg aggttatgca gtgttaagtt ttcaattgct attttcactg
     5821 tttgttattg cgcttatcat tgcattgata agtggcttgt tgtttttagc accagttatg
     5881 ccaatgagat atattaaatt acatttatac atactagtca tgccagtatt atttgcagtc
     5941 attggctttt tcggtattca tggtcaacat gtcttaggtc catttaaaat agatcgttta
     6001 tcttggttat tagctggctt tgtaatggcg cttggtttta ttattcaaaa gttttcaatg
     6061 cgatatttac taggtgatca tcattataga cattactttc cattgttcac tgcgattacg
     6121 tcgtttgcat ctttagcatg gatgtctgaa gacttaagac tgatggcact ctgctggggt
     6181 atgacattat tatgtttaac attgctgatg aacgttaatc gtttttggaa agtgccacgt
     6241 gagtctgcga aattatcaag tatgacattt ttatgtggtt ggcttgcatt cgttggagca
     6301 attgtaacta tttatattgc gactggcgag tggcgcgtgc ctcaacatat gtctaattcg
     6361 acatggtcat tgttgacgaa tgtactactt gtattagctg tcatgatacc agcagcacaa
     6421 tttccatttc atcgatggtt gattgaatct gtaacagcac caacgccagt atcggcaatt
     6481 atgcatgcag gaattgtgaa tgcaggtggt gttattctaa ctcgttttgc gccgatattt
     6541 gataatggat ttgcgttatc attattactt atcctttcta gtatttctgt attgttagga
     6601 tcgggtatta gcttggttca agtggattac aaaagacagt tagtcggctc tacgatgagt
     6661 caaatgggct ttatgttagt tcaatgtgca ttgggtgtat attcagcagc gattattcat
     6721 ttaatattgc acggtatttt taaagcaaca ttatttttac aatcaggttc tatcgtgaag
     6781 cgattcaata ttccaaaaca agcatctgct aaagacgctt atggctggat tgtcatggga
     6841 cgtgtattag ctattatcgt ggcatttcta ttttggatga gtagtgacag aagtgcatat
     6901 gaagtgttaa gtgcactcat tctagcatgg tcattacttg tatcttggaa tcagatggta
     6961 gcctttagta aagggcgcat ggcacgtctg gttggcatga ttttgattgc aattgtgaca
     7021 tttatctacg tcatcacaca caattatttt tacgatgtat tacaaaatat aacaacacat
     7081 gcgacaacgc cgcctacagt gagtgtcatc attagtgttg tcattttaat atttggtagt
     7141 ttattaagta tttgggtggc gcgtcatcga tactctaagg gttttgcggt attgtacgtg
     7201 tggttagtta atctaggtga agcacgctcg aaagcgatag aaagtcatcc gaattatttg
     7261 aagaagtatt tataggaggt gaaaggtatg acaacacagt taaatatcaa ttcagtcatt
     7321 gaaaatgcga aacgtgttat tacaccatta tcaccgattt cgatttttgc agcacgaaat
     7381 ccatgggaag gattagaagc ggatacgttt gaagatgttg caaaatggtt acgtgatgtt
     7441 cgagatgtgg atatttttcc aaataaagca ttaatagaaa gtgctgtggc acgtggtgaa
     7501 ttagacgaaa gtgtctttaa tcaacttgtt actgatatgt tacttgaaca tcactacaat
     7561 atcccgcaac actacatcaa tctttatatt gataacatta aaacattaaa agacgtacct
     7621 gcatcatata tgaatcattc aaatgttgat gttgttgctg atctactatt agaaaaatca
     7681 aaacgtgata tggctgattc atatcatcac tatgatgtac gtccgatgag tgatgcaata
     7741 atagatgaac aaggtgagcc acttagcgaa caagtgaatc gtcaaatgat taaatggacg
     7801 aaactttata tcgatcaatt tctatcgagt tggacaatgc cgaagcgtga gcaaagtttt
     7861 taccatgcat ggttgcattt agcgcaacat gatcatagtt ttactaaagc acagcgccaa
     7921 gtgattaaag gcttgcccaa tgatcctaaa atgacgatag agtcagtatt aactcatttt
     7981 tcaatagatc aggaagacta ccaagcttat gttgaaggac atcttttggc gttaccgggt
     8041 tgggcaggta tgttgtatta ccgttcacaa cagcatcact ttgaacaaca tttgttaacg
     8101 gattatttgg caattcggtt agttgtcgaa caattgctag ttggtgatga gtttaagtca
     8161 gtcactaaag attgtgaaag tagatcggaa aattggttta agcaaactgt tgcatcatgg
     8221 tgttactaca gtgatatgcc tagcgatgta ttactacaac atgacgtcaa tgaaattcaa
     8281 acgtttattc attttgcagc aactatgaat aaaaatgtat ttaaaaattt atggctaatt
     8341 gcctgggaaa tgacatacga atctcagtta aaacaaaaaa ttaaagcagg tcatgaaagt
     8401 gtggcgggcg cattagatgt aaaccaagta aatgtctcag aaaatgataa cgctaatcag
     8461 ccacattcag tattattaaa tgacacacaa gcagttgatg aaaataatag cgagctaaat
     8521 caggtgggca catcaacgaa agcgcaaatt gcattttgta tagatgttcg ttcagaacca
     8581 tttcgtagac atatcgaagc agcagggccc tttgaaacga ttggtattgc aggtttcttt
     8641 ggattaccta ttcaaaaaga tgccgtagac gaacaattca aacatgattc attacctgtc
     8701 atggtaccgc cggcatatcg cattaaagaa tttgcagacc gctacgatat gaatgtttat
     8761 cgacaacagc aacagacaat gtcatcgatg ttttacacat ttaaattgat gaaaaataat
     8821 gttatgccta gtctgttatt gcctgaatta agtgggccat ttttaagctt gagtaccatt
     8881 gtcaattcga ttatgcctag aaaaagtcgc gcgtctttac aaaaaataac acaaaaatgg
     8941 ttgaaaaagc ctgaaacaaa gttaacgatt gatcgtgagt ttgaccgaac atcagactta
     9001 cctgttggat ttactgagca agagcaaatt gatttcgcgt tacaagcgtt gaaattgatg
     9061 gatttaaccg aagcatttgc gccgttcgtt gtgttagcag gtcatgctag tcattctcac
     9121 aataatccac atcatgcatc acttgaatgt ggggcttgtg gtggcgcatc aagcggtttt
     9181 aatgctaagt tattagcgat gatatgtaat cgtccaaatg tcagacaagg attaaaacaa
     9241 tcaggtgtgt atattccaga gacaactgtt tttgcggcag cagaacatca tacgtctact
     9301 gatacgttgg catgggtata tgtgccagac acattatctt ctattgcttt agatgcatat
     9361 gaatcattga atgacgcgat gccgatgatt tctgaacacg cgaatcgcga acgtttggac
     9421 aaactgccaa cgattggtcg tgtgaatcat ccagtggaag aagcgcagcg gtttgcgagt
     9481 gattggagtg aggtacgtcc agaatggggc ttggctaaaa atgcatcatt tataattgga
     9541 cgacgccaat taacaaaagg cattgattta gaagggcgga catttttaca caattatgat
     9601 tggcgtaaag ataaagatgg caaattgtta aatactatca tttctggtcc ggcgcttgtg
     9661 gcacaatgga ttaatttaca atattatgcg tcgacagttg cgccgcattt ttacggaagt
     9721 gggaataaag cgacacaaac cgtcacgtca ggtgttggtg tcatgcaagg taatgcgagt
     9781 gatctgatgt atggcttatc atggcaatct gttatggctg ctgatcgaac gatgtatcat
     9841 tcgccaattc gtttgcttgt cgttgttcag gcacccgact atgttgtagc aagactactc
     9901 gcgaataatg accatttcgc taggaaggtg tctaatcatt ggctgcgttt aatgagcgtt
     9961 aatgaggaag ggcgttttaa aagttggatt taacgatgat atgttgcgat gctaagtaac
    10021 taatatatca tcatatctac aatgttctag cttcttaaaa tatactaaaa atgataaact
    10081 taatgtaata gttgatgtcg tgataacgat acaaagatga agtaatcaat tttaatatta
    10141 ttggagttag tgatatgaaa agaacgaaag gtgaaatcga agctgaaatc agtaaagcca
    10201 ttacgcaatg ggaaaaagat ttccttggca gaggttcctt gtcagttaaa tcagacattt
    10261 taagagatat ggtgattatt agtttacaag gtatcttaac gccagcagaa tatcgtgtgt
    10321 gtagtacgaa tgaaggatta ctaaatatta aacgaacacg ttctgaatta gttgaatccg
    10381 gtgagcaaga tttgaatgat atcattttta aaattacagg tatcaaagtg atgagcttcc
    10441 atagtgattt aagtacagtt acaggtgaac gtattatcgt attcaaactt gaggataatt
    10501 tggaaaagca tatttaagaa ggttaggtat gcttttgctg gcttattaaa gctatagaag
    10561 tatgcttggt taaacagcgc aacgccatat atttgggtgt gctgtttttc tattacatga
    10621 tgtgtttatt acgtggatga atgcgtgggt ctgaatatag cccgtgaaag gcgtatgtca
    10681 taaaatttgc gaatggtgac aagtcgacgc aagcatattt gttgcgagac atgcccacta
    10741 tccttttaca taaagagtat atgtgtgacg taggcatata atcgataaag tattcctaaa
    10801 aaattaggta taatgaccat tgttgcaata agtttttcgg ccttgaaacc gataacgcat
    10861 agtataatag gaataaaata taatacaaat atccagtaaa tcaaatttga aaacgatgga
    10921 tgaatagggg taaagaatct aactaatgta atgattaacg ctatatagac aaaaataact
    10981 ctattgatac gccttacccc ctctgtataa taaatataga tcgtactaaa ttgaaaatag
    11041 caaaatattg ttatttcaat tatacaatgt tttatttgca atatacataa caatatgaac
    11101 tttataattt tgatattgtc ggtaaagtga aatttgtggc acattgatat tatggataac
    11161 atttgtaaaa tgaaaatgat gatacgagga gattgtaaga tgatagataa aaaattaaca
    11221 tcaccgaaaa tgacagtgcc tttattttta atcgcgctga ttgtatttat aggtatgttt
    11281 tacagtgtag tgacaaatca agaatggctt aaaaatatag atatgggatc attaacatgg
    11341 tttacagatt atttcggtga gccacaacgt cagtatgtta acaatttatt taattactat
    11401 atgacgttta gtgcggaaat tggagatgtc aaaggtgtcg tgttgatttc cattatcgtc
    11461 acaatcatac tgtttattaa acagaggcat ttagcggttt ggtttgtgac atatttggtt
    11521 tcaggtgtca tcatgaacaa attaattaaa gatactgttt tacgtccaag accatataat
    11581 catttagccg ttgatacagg cttttcattt ccaagtggac attccaacgc cagcacatta
    11641 ttatatttcg ctttaatgat cataattatt tcacttgctg ctaagacaat aacaaaagtg
    11701 ttgagtgcgc tagttatggg aatattatgg cttagcatat tattttgtcg cctttatttt
    11761 catgcgcatt acttttcaga tgtcattggc ggcacgtcac tagcaatcat ttgggtagcg
    11821 ttattcttaa tggtataccc atactttatt aatcatcgac gacaacgcgt ttagcaaaca
    11881 tctttagaca tagtggaggt atatagaaaa atgagaatta aaacaccgag tccctcttat
    11941 ttaaaaggca caaatggaca tgcgatatta ttattacatt catttacagg tacaaatcgg
    12001 gatgtgaagc atcttgcagc tgagttaaat gaccaaggat ttagttgtta tgcaccgaat
    12061 tatccaggtc atggcttact gttgaaagat ttcatgacat ataatgtaga tgattggtgg
    12121 gaagaagttg agaaagctta ccaattttta gtcaatgaag gttatgaatc tatcagtgca
    12181 acgggtgtgt ctttaggtgg attaatgaca ttaaaattgg cgcaacacta tcctttgaaa
    12241 cgtatcgctg tcatgtcagc accaaaggaa aagagtgacg atggtttaat agaacattta
    12301 gtttattata gtcaacgcat gtcggatatt ttaaatttag atcagcaagc atcgagtgcg
    12361 caattagcag caattgatga ttatgaaggt gaaattacga agtttcaaca ttttattgat
    12421 gatatcatga caaatttaaa tgttattaaa atgccagcta atatattatt tggtggtaaa
    12481 gatgcgccat cctatgaaac aagtgcacat tttatttatg aacatttagg atcagtagac
    12541 aaagaattaa atggtctgaa ggattcgcat catttaatga cgcatggaga aggcagagat
    12601 attttagaag aaaatgttat tcgctttttc aatgctttaa cataattgaa gattttaaaa
    12661 attaacactt gagtgacgat gttattatat ttgtcatttg agtgtttttt gtggcgtgat
    12721 agtataggga acttagctaa agttaatttt tagtgtgatg tcgtggaaat attatggagt
    12781 tataccagaa gttgatttgt attgtaatgt tgtgggcatt ttatgggcat tgcacctgaa
    12841 gttgttatgt ggcgagatgt tggggatata gatggtgttg tatgtggcga gatattggcg
    12901 cacgtataca gcgttgaacc aggaatttta atgtagcgta atgacaacac attacaagag
    12961 ttgactatat tatgataaat ccaggcgcta ccgtattaat gaaacaattt tgatgcaggt
    13021 gtattgcatg ttatttacat acatgttaga gtgttactta aacacgtaga cgctttaacg
    13081 ctttaaagga gatgtgaatg atgaaaagac aacaatcaca atggaagtca tcaactggat
    13141 ttattttagc tagtgcgggt tctgcaatcg gtcttggtgc catgtggaaa ttcccatata
    13201 tggcagggat ttatggcggc ggtgcctttc tagctatgtt cttaatattc accatttttg
    13261 ttgggttacc attactcatt atggaattca ctgttgggaa aatgggacgg acatatacaa
    13321 cacaaatata tagtaaatta actggtaaaa aatggctcaa tatcattggc tggaacggta
    13381 atttggccgt gtttatttta tttggcttct atagtgttat cggtggttgg attgtcattt
    13441 acatcggaca agtattatgg caattagtta tatttcaacg catcaatcat ctccaagaaa
    13501 tgaattttga agcggtaata tcaaatcctt ggttaactgt tctagggcaa ggtatattca
    13561 tattcgctac gatgattatt gtcatgttag gtgttgaaaa aggattagaa aaggcatcga
    13621 aagttatgat gccattgctg tttgtctttt taatcatcat tgtgattaag tctttaacat
    13681 tagatggcgc cttagaaggt gtgaaattta ttttacaacc aagagtgtca gagattactg
    13741 ctgacggtat cttgtttgcg ctaggccaat cattctttac gttatcatta ggaactacag
    13801 gtatgattac ttatgcgagt tatgcctcta aagacatgac gattaagtca tcagctattt
    13861 ctatcgttgt tatgaatatc tttgtatctg tattggcagg tctagctata tttccggcgt
    13921 tacatagttt tggctatgaa ccacaagaag ggcctggatt attatttaaa gtactgccaa
    13981 tggtctttag tcaaatgcat ctaggcacat tattctattt gggattctta gtgctgttct
    14041 tatttgcggc tttaacgtca tctatttctt tattagaatt aaatgtttct aacttcacga
    14101 agaatgacaa tacaaaacgt aaaaaagtcg cagtgatcgg tagtatttta gtatttatca
    14161 ttagtattcc agcaacctta tcttttggta tcttaaaaga tgtaagattc ggtgcgggaa
    14221 cgatttttga taatatggat ttcatcgttt cgaatgtatt gatgccatta ggcgcattag
    14281 gtactacgct tgtcgtagga caattattag ataaaaaatt attacaacaa tattttggta
    14341 aagatcgatt tagattattc agtggttggt attacttaat taagtatgcg atgcctgtcg
    14401 ttattatttt agtctttatc gtgcaattat ttagttaata tatagaagca tctgacacac
    14461 aagtatttgt gttgctcagg tgtttttttg tttggaattg aaaagttagt actggttctg
    14521 aattaatcga aacttagata attgcaaaat aacaaaatgt cgtttataat tcttatctga
    14581 aaagtaagaa ttaaaattga gacatgaaga aaggtttcat tcatttgatc ggcatgtcag
    14641 atgagggtgc atctatgatt acttatgatt taattggcaa tacaccatta gtactgttag
    14701 aacattatag tgatgataaa gttaaaattt atgccaagct tgaacaatgg aatcctggag
    14761 gcagtgttaa agacagactc gggaaatatt tagtagagaa ggcaattcaa gaagggcgtg
    14821 tgcgtgcagg tcaaactatt gttgaagcga ctgctggtaa tacaggcata gggttagcta
    14881 ttgcagcgaa tagacatcat ttaaaatgta agatctttgc gccgtatggt ttttcagaag
    14941 aaaagattaa tattatgata gcgcttggtg cagatgtttc aaggacgagt cagtctgaag
    15001 gtatgcatgg ggcacaatta gctgcacgtt cctatgctga aaaatatggt gccgtttata
    15061 tgaatcaatt tgaatccgaa cataatccgg atacatattt tcatacattg ggacccgaat
    15121 tgacttcagc attacagcaa attgattatt ttgtggctgg tattggctct ggcggtacat
    15181 ttacaggtac cgcacgttat ttaaagcaac atcacgtgca atgttatgcc gttgagccag
    15241 aagggtccgt gttaaatgga gggccagctc atgcacatga cactgaaggt atcggttctg
    15301 agaaatggcc gatattttta gagagacgtc ttgtagatgg gatatttacg attaaagatc
    15361 aagatgcctt tcgaaatgtc aaaagtttgg ctataaatga agggttgtta gtaggcagtt
    15421 cttcaggtgc agcattacaa ggtgcattga atttaaaagc gcaattatct gaaggtacga
    15481 ttgttgtcgt atttccagat ggtagcgatc gatatatgtc taagcaaata tttaattatg
    15541 aggagaatga ttatgaataa gaaaactaaa ttaattcacg gtggacacac aacggacgac
    15601 tatacaggtg cagttactac accaatttat caaacaagta catatttaca agatgatatt
    15661 ggtgatttac gtcaaggata tgaatattct cgtactgcga atccaacaag aagttctgta
    15721 gaaagcgtta ttacggcatt agaaaatggc aaacatggct ttgcgtttag ttcaggtgtt
    15781 gcagctatca gtgcagttgt tatgctgttg gacaaaggtg atcatattat tttaaattca
    15841 gatgtatacg gcggtactta tcgcgcattg acaaaagtat ttacacgatt tggcattgaa
    15901 gtggattttg tagatacaac gcatacagat tcaattgtac aagcgatacg cccaacaaca
    15961 aagatgttgt ttattgaaac accttctaat ccattattac gtgttactga cattaaaaag
    16021 tctgctgaaa ttgcgaaaga acacggtttg atttcagtcg ttgataacac atttatgaca
    16081 ccttattatc agaatccatt agatttaggc atcgatattg tcttgcattc tgcaacgaaa
    16141 tatttaggtg gacatagtga tgtcgttgct ggtttagttg caacatcgga tgacaagctt
    16201 gcagaacgtt tagcatttat ttcaaattca acaggtggca ttttaggacc tcaagatagc
    16261 tatttacttg tgaggggtat taaaacatta ggtttacgta tggaacaaat taatcgcagt
    16321 gttattgaaa ttattaaaat gttacaagca catccagctg tgcaacaagt gttccatcca
    16381 agtattgaaa gtcatttaaa tcatgatgtc catatggctc aagcggatgg ccatacaggt
    16441 gtgattgcat ttgaagtgaa aaatacagaa agtgccaaac aattgattaa agcaacatcg
    16501 tattacacat tagctgaaag tttaggtgca gtggaaagtt taatttcagt acctgcattg
    16561 atgacacatg catccattcc agcagatatt cgagctaaag aaggtattac agacggactt
    16621 gtaagaattt ctgtaggtat tgaagatact gaagatttag tcgatgattt aaaacaagca
    16681 ctagatacgt tataaataat agcagcactg gcatatattt tgagttcaac gtttgttgtt
    16741 cttgaatatg ctagtgcttt ttttgtgcga tgttaatcat tcgtgattga gttatggagg
    16801 tttggtggac atttgtatgg atttaatttg ataaatgtca tagtagtctc acaattcgtc
    16861 attgtcacat gaattgctta ttttttattt ttttagaaaa ttcggcaaat tgtattgacg
    16921 atatatgcaa ttttacatat aatgctttta ttcctagtgg aatgataaga atttgaagga
    16981 gggggctttg catgattgag tttcgacagg taagtaagac ctttaataaa aagaagcaaa
    17041 aaatagatgc tttgaaggac gtatcattta cggtcaatcg caatgatatt tttggtgtga
    17101 ttggatatag tggtgcagga aaaagtacgt tggtaagact cgtgaatcat cttgaagctg
    17161 cctcgaatgg acaagtgatt gtagatggac atgatattac gaattatagc gataaaatga
    17221 tgagggatat taagaaagat atcggtatga tatttcagca tttcaattta ttaaattcag
    17281 ctaccgtatt taaaaatgta gcaatgccac tcattttaag taagaaaagc aaaacagaaa
    17341 ttaagcaacg agtaacggaa atgcttgaat ttgtaggatt gagtgataaa aaagaccaat
    17401 ttcctgaaga attatctggt gggcagaagc aaagggtggc tattgcaaga gcgcttgtta
    17461 ctaatccgaa aatactccta tgcgatgaag caacaagcgc attggatcca gcaacgactg
    17521 cttcgatatt gacgttatta aagaatgtca atcaaacctt tggcattaca attatgatga
    17581 ttacacatga aatgcgcgtt attaaagaca tttgtaatcg tgttgctgta atggaaaagg
    17641 gtcaagtggt tgaaacagga actgttaaag aggtgtttag tcatcctaaa acgacgattg
    17701 ctcaaaattt tgtgtctaca gttatacaga ctgagccaag tacatcattg attcgtcgat
    17761 tgaatgacga acaagttggc gattttaaag attataaaat cttcgtcgag gaaactcagg
    17821 tgacacaacc gattataaat gacttgattc aaatttgtgg cagagaggtt aaaattttat
    17881 tttcatctat gtcagaaata caaggtaaca ccgtatgtta tatgtggctt cgatttaata
    17941 tggatcaaca atttgatgac acggcaataa atcaatattt caaagagaaa aatattcaat
    18001 ttgaggaggt gcattaacat atgtttggtt ctgatttaga cagtgcacag ttattacaag
    18061 cattgtacga aacgctatat atggtatcta ttgctttatt tttaggagca gtgattggta
    18121 ttccattagg tgtcttattg gtaattactc gaaaacaagg catatggccg aatatagtga
    18181 tacatcaagt tttaaatcct ataatcaata ttttaaggtc actaccattt attattttgt
    18241 taattgcgat tgtaccattc acaaaattag tagtaggtac ttcaattggt acgactgctg
    18301 ccatcgtgcc tttaacagta tatgtggcac cttacattgc aagacttgtt gaaaactcat
    18361 tattagaagt agacgagggg attattgaag cggcgaaagc gatgggcgct tcaccactac
    18421 aaatcattag atatttttta attcctgaag cgttaggttc attagtatta gcaattacca
    18481 ctgcgattat tggacttatt ggaagtacgg cgatggcagg agctgttggc ggtggtggta
    18541 taggagactt agctttagtg tatggttatc aaagatttga tacgacggtc attattatta
    18601 ccgttattgt attagtcatt attgtccaag tgattcaaac gctagggaat gtcctagcta
    18661 gattcatacg tagacattaa tgatatatag tgaagatttt gaaaggaatt gatagaatga
    18721 aaagattgat tgggttagtt atcgtagcac ttgtattatt agcagcgtgt ggtagtaaca
    18781 atgataaaaa agtaacaatt ggtgtcgcat caaatgacac taaggcttgg gagaaggtta
    18841 aagaattagc taaaaaagat gatattgatg tggagattaa gcacttttcc gattacaatt
    18901 taccgaataa agcattaaac gatggtgata ttgatatgaa tgcattccaa cattttgcat
    18961 ttttagatca gtataagaag gcacataaag gaacaaagat ttcagcattg agtacaacag
    19021 ttttagcacc gttgggcatt tactcagata aaattaaaga tgtcaaaaag gttaaagatg
    19081 gtgctaaagt tgtcattcca aatgatgtat caaaccaagc acgtgcactt aaactattag
    19141 aagcagctgg tttaataaaa ctgaaaaaag atttcggatt agcaggtaca gtgaaagcta
    19201 taacgtcaaa tccaaaacat ttaaaaatta ctgcagtaga tgcacagcaa actgcgcgtg
    19261 ctttatctga tgtcgatatt gcagttatta ataacggtgt agcaactaaa gcggggaaag
    19321 accctaaaaa tgatccgata tttttagaaa aatcaaattc agatgctgta aagccatata
    19381 ttaatattgt tgcagttaat gacaaagact tggataacaa aacatatgct aaaatcgtag
    19441 aattgtatca ttcaaaagaa gctcaaaaag cgttgcagga agatgtcaaa gatggagaga
    19501 aacctgttaa tttatctaaa gatgagatta aggcaataga aacgtcatta gcaaaataaa
    19561 ttatattgcg tcctacaagc aaagttcatg cttatgtttg tagggcgtta ttgttggaga
    19621 ataaaattat ttccaataga gaaagggatt gtaatcattt tatagtgaaa tattatgaaa
    19681 ttgtaataat ttagatattg taaaatctaa taagttgtaa taattttaag gggtaattat
    19741 aaaatttgat gatacagtat atgatttttt tgtaatcata atgtcatcaa acatcaacct
    19801 attatacata ataaaatcgt ataatgatgt agtattcata aattcggata aaagaatgtt
    19861 aggaaagtta agcaagagga ggattttaaa gtgcaaaaaa aagtaattgc agctattatt
    19921 gggacaagcg cgattagcgc tgttgcggca actcaagcaa atgcggctac aactcacaca
    19981 gtaaaaccgg gtgaatcagt gtgggcaatt tcaaataagt atgggatttc gattgctaaa
    20041 ttaaagtcat taaacaattt aacatctaat ctaattttcc caaaccaagt actaaaagta
    20101 tctggctcaa gtaattctac gagtaatagt agccgtccat caacgaactc aggtggcgga
    20161 tcatactaca cagtacaagc aggcgactca ttatcattaa tcgcatcaaa atatggtaca
    20221 acttaccaaa acattatgcg acttaatggt ttaaataatt tctttattta tccaggtcaa
    20281 aaattaaaag tatcaggtac tgctagctca agtaacgctg cgagcaatag tagccgtcca
    20341 tcaacgaact caggtggcgg atcatactat acagtacaag caggtgactc attgtcatta
    20401 atcgcatcaa aatatggtac aacttatcaa aaaattatga gcttaaatgg cttaaataat
    20461 ttctttatat atccgggtca aaaattgaaa gtaactggta atgcatctac gaactcagga
    20521 tctgcaacaa cgacaaatag aggttacaat acaccagtat tcagtcacca aaacttatat
    20581 acatggggtc aatgtacata tcatgtattt aatcgtcgtg ctgaaattgg taaaggtatt
    20641 agtacttatt ggtggaatgc taataactgg gataacgcag cggcagcaga tggttacact
    20701 atcgacaata gacctactgt aggttctatc gctcaaacag atgtaggtta ttatggtcat
    20761 gttatgtttg tagaacgtgt aaataacgat ggtagtattt tagtttcaga aatgaactat
    20821 tcagctgcac caggtatttt aacttacaga acggtaccag cttaccaagt aaataattat
    20881 agatatattc actaaagtct tacgtatata aatatataat gaattcctat tacatttcac
    20941 aagctgaaaa gttggattga atcctaaact ttatggcggg gctatcaaag tcgtgattgt
    21001 tgtaatagga atttttgtat aagaaaaagg attggtcgaa tgaacttcat gttctatgtt
    21061 cgaccaatcc ttttctgaat tagtattcat gctttacttt gcgaatgctt gtaaataatc
    21121 tagcaccgtt tgttattaaa gtaacaactg ccactgcttt tttgaactta cgtggtgact
    21181 taattgaaat gtaaaagtct aacacggttc ctacagtgct taaacctaat gaaagatata
    21241 ctaatttttt atttttagca tgatatttat agccattgta gccgtcgact atgaaacctg
    21301 cgacatttag taaacttgat aaacgttgtg atttggaacg ttttgccata ataataattc
    21361 cccctagtat cttctgttta tatgaaaaat tgggtgcgct gaattgctaa cgttttgcgc
    21421 tataactact catatatgat aacataattg tacagtataa ttttaaaaat tgatttcaca
    21481 aagttggggt gtcaaagatg attaaatgtg tctgtttagt tgaagaaaca gctgataaaa
    21541 tattacttgt tcaagtaagg aatcgcgaaa agtattattt cccaggtggt aaaatagaag
    21601 aaggggaatc acgagtacaa gcgctgttaa gagaagtaaa agaagaatta aatttaacat
    21661 taacaatgga tgaaattgaa tatatcggga caattgtagg tcctgcatat ccacaacagg
    21721 atatgttaac tgagttaaat ggatttcgcg cattaaccaa aatcgattgg gaaaacgtaa
    21781 ctatcaataa tgaaattacg gatatacgct ggattgataa agataatgat gcgttgattg
    21841 cgcctgctgt caaagtttgg attgaaacgt atggtggtaa acatgacaaa taatgacacc
    21901 atcatgttac gacattatgt cccacaagat tattcgatgt tagaagcttt tcaattaagt
    21961 gaaagtgatt tgaagtttgt taaaacgcca gaggaaaata ttacagctgc aatgtctgat
    22021 aatgaaaggt atcccatcgt tgtaatggat ggcaggcaat gtgtggcctt ttttacatta
    22081 catcgtggaa aaggggtcgc accatttagc gataaccaag atgcagtatt tttcaggtca
    22141 tttagtgttg atcaacgtta tcgtaataga ggaataggta aagtggtaat ggaaaaattg
    22201 gcgtcattta tcacttcaac atttcaggat attaatgaga ttgtgttaac ggttaatact
    22261 gacaatccac atgccatggc actttatcgc caacaaggat atcaatatat gggagatagt
    22321 atgttcatcg gaagacctgt tcatattatg gcgttaacta taaaataaat taaatttaaa
    22381 agcatcttta ctcatcgtcg accacaacaa ttaatgatga ataaaggtgc tttttgttat
    22441 agatcatcgg acaatttact atagtaaaaa gcgacctagt gaacaattga catatatcca
    22501 caggtcgctt aacttaagtt atattgctag ttgcgattaa ttgatagact catcattttt
    22561 gcgctgtcga gatggtcttt ttattaaaaa tgccgtaatc caagccgtaa tcggaatact
    22621 gattgcaacg gcaataccgc ctaaaataat agaaataaat tcttgggcaa atattttcga
    22681 gtttataata tgaccaaatg aatatttaag tttgaaaaac caaataaata aagcaagttg
    22741 gccaccaaaa aaggcaaggt aaatcgtgtt cgcagatgtc gctaaaattt ctctaccaac
    22801 acgcatgcca gattggaata attcgtattg cgtaagcgtt ggattcactt gatgcaattc
    22861 ataaatgggt gaactaatgg taattgttaa atctatcaca gctgcaataa cagcaagaat
    22921 aatagtgaac accataaatt gaaccatatc aatgccaata ttcattgaat acacatatgt
    22981 ttcatcttgt tgttcggttg aaaagccttg tagatgaccg aagtagaccg ataaataaat
    23041 gagtgtaatc aacaatattg ttgtaacgat agtgctgata aatgcagctt gtgttttaac
    23101 attgtaacta ttgagtacga ataaattaca agcgccaata ataatgcaga aaaagaatgt
    23161 gacgacataa atcggtacgc caaaaataat caacacaata ctaataatta aaatagcaaa
    23221 atttaaaaat agggttaaat aagagatgaa tccctttcta cctccgaaaa ttatcatcag
    23281 aaagaggagc aataacgcca atataaatac agcattcatt gtttcgccct ccttaatgtt
    23341 tcaaatattt ccataaacaa tattgtgata ggaattgtaa gtacgatacc tataccacct
    23401 gttagtgcgc gcgcaatttc taacgaccaa ttcatcgaaa tagtataagt cactgtattg
    23461 gcatttttta aaaagattaa aaacataggt agtgcaccgg ataaatatga gaataataag
    23521 atgttagtca ttgttcccat aatatcttgg ccgatgtttc ggccagcaag cgcccatctc
    23581 ctcattgaaa tgtgtggcgt acgctgtaaa atttcatgca taccactagc aattgtaatt
    23641 gcaacatcca taatagcgcc aagtgaacct attaacactg aggctaggaa gatatctttc
    23701 ggtggtaatg ataaaaagtt catcgtttca tatttaatgc ctttaccgtc tgtcatatat
    23761 atgattaatt ctgttaaacc tatactcaaa aaagttccga taattgtact ggctatggta
    23821 atgagtgtgc gcatatgcca gcctgtaacg agcaataaag tgagtattgt tgaacagatc
    23881 atggcaatgg tcatgagtaa gaataaatta atattgctat gttgaatatg aatgtaaatt
    23941 gcgattaata tggcaataga attcaagatt aacgataaaa tcgattgcag tccgactttg
    24001 cgaccaacca ataatacagt taataagaac aaaccagtga tgataaccgt taaggtatca
    24061 cgattctttt ctataatata agcatcactc ggcttgttag aaatatgtaa taatactttt
    24121 tcgtgtgtgc gaaatgcctc agaatctgct tgcgatttga cgtactgatg attaatcgtc
    24181 gtcgtttctc cagcaaattg accatttaat attttgactt ttaattgatt tttatattta
    24241 atatcacgat tattttgtgc atcttttgta ggtgtcgaag aaacatgttt gacatctata
    24301 atttgaccaa ttggtttgtt gtaaaagttc tcattattga atgtaaataa aatagcacca
    24361 atgaatgcga tgcagaacaa acctaaaatt atattaaatg gctttgtaaa taaatttcta
    24421 tatttcaaaa acaaaacccc aattctatga atgaattaat atggtgatta tacgccctta
    24481 attttttatt ttcaaagata ttactgctaa gtgtaaaacg aaaatcatca ttgatagcat
    24541 cgaattactt aatggaatgt agacgtttta gtcattaatt gctgaatgag tgttaataag
    24601 ataccaatat cactcttcgt ataaggttct ttcgtaatag cacatatcgt tctttttaat
    24661 tcagtatgat ctaattttat atctatccat gatttagatt ctggtaaatg tatattttgt
    24721 gatgaaatga tgtaaccttc tttttgacga aggagatact gcgcaagtgg ttggctactg
    24781 attgtgtata catctgattt agtaatcttg cgcaattgtt tttttacagt ttcggcaaat
    24841 ggtgccaagc aataaatatg actatgctca aactgaatta atggtgggtg tgtcgccatc
    24901 gtaattggat cgtctgaagg cgcatataaa tgatagtgct cttcgaataa aggtagcata
    24961 tgtaattgtt tgtgtttacg tatttctggt gtaagttccg tgaaaccaat gtctatattc
    25021 ccatttaata cgctatttat aatcgtgtca tgatctaata agttcggtat gacatgtgta
    25081 tcattttgta aatgaaacgt ttggataagt ggtagtaaca tgtgggatac gtcactctca
    25141 tcatagccaa tgtagatact tttattttta gttaatccat ggctttgaaa ttgttcaatc
    25201 gtgctatcta aatgttcaat aatgcgcaga gcttcattaa ataataattt cccttcagaa
    25261 gtgagcgtaa tattgcgtcc ttgcttttta aataaagaca cattaagttc ttgttctaat
    25321 aatgtaattt gacggcttat cgctgattga gcaatgttta gttcaagtgc tgtttcggag
    25381 atatgttctc ttttagcgac ctcgataaaa tatcttaatt gtttaatttc catagtgata
    25441 taggcacctc caaaaatgag tgttttgtaa ctattatagc aatattattg ataaatgttc
    25501 tattttttag atgaatatct tctat
//
LOCUS       DBNENW010000021        25399 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNENW010000021 DBNENW010000000
VERSION     DBNENW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.021"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..491
                     /locus_tag="AC1LW8_000703"
     CDS             <1..491
                     /locus_tag="AC1LW8_000703"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIP4772568.1"
                     /translation="ETEQLVKPVKIIPLEVVVRNIASGSITKRLGFENGEVFREPLVE
                     FFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLKQLMDAMNLKLVDFK
                     IEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGSLIETYQIFLNKLED
                     LK"
     gene            491..754
                     /gene="purS"
                     /locus_tag="AC1LW8_000704"
     CDS             491..754
                     /gene="purS"
                     /locus_tag="AC1LW8_000704"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIP4772569.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            756..1427
                     /gene="purQ"
                     /locus_tag="AC1LW8_000705"
     CDS             756..1427
                     /gene="purQ"
                     /locus_tag="AC1LW8_000705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HIP4772570.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            1420..3609
                     /gene="purL"
                     /locus_tag="AC1LW8_000706"
     CDS             1420..3609
                     /gene="purL"
                     /locus_tag="AC1LW8_000706"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIP4772571.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            3588..5072
                     /gene="purF"
                     /locus_tag="AC1LW8_000707"
     CDS             3588..5072
                     /gene="purF"
                     /locus_tag="AC1LW8_000707"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIP4772572.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            5065..6093
                     /gene="purM"
                     /locus_tag="AC1LW8_000708"
     CDS             5065..6093
                     /gene="purM"
                     /locus_tag="AC1LW8_000708"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIP4772573.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDSSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIMKNES
                     TAIELLGV"
     gene            6096..6662
                     /gene="purN"
                     /locus_tag="AC1LW8_000709"
     CDS             6096..6662
                     /gene="purN"
                     /locus_tag="AC1LW8_000709"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIP4772574.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVSLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            6677..8155
                     /gene="purH"
                     /locus_tag="AC1LW8_000710"
     CDS             6677..8155
                     /gene="purH"
                     /locus_tag="AC1LW8_000710"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIP4772575.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            8177..9424
                     /gene="purD"
                     /locus_tag="AC1LW8_000711"
     CDS             8177..9424
                     /gene="purD"
                     /locus_tag="AC1LW8_000711"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIP4772576.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFAIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     AAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKNG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(9417..9542)
                     /locus_tag="AC1LW8_000712"
     CDS             complement(9417..9542)
                     /locus_tag="AC1LW8_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772577.1"
                     /translation="MHNHKFGTEINVLDSTTKKAMKKSIVIDSFIANLSILNLLI"
     gene            complement(9689..10495)
                     /locus_tag="AC1LW8_000713"
     CDS             complement(9689..10495)
                     /locus_tag="AC1LW8_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP4772578.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(10488..11888)
                     /locus_tag="AC1LW8_000714"
     CDS             complement(10488..11888)
                     /locus_tag="AC1LW8_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772579.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(11903..12478)
                     /locus_tag="AC1LW8_000715"
     CDS             complement(11903..12478)
                     /locus_tag="AC1LW8_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP4772580.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(12555..12655)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13125..13259)
                     /gene="graF"
                     /locus_tag="AC1LW8_000716"
     CDS             complement(13125..13259)
                     /gene="graF"
                     /locus_tag="AC1LW8_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HIP4772581.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            13411..14706
                     /gene="auxA"
                     /locus_tag="AC1LW8_000717"
     CDS             13411..14706
                     /gene="auxA"
                     /locus_tag="AC1LW8_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid stability factor AuxA"
                     /protein_id="HIP4772582.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            15131..16303
                     /locus_tag="AC1LW8_000718"
     CDS             15131..16303
                     /locus_tag="AC1LW8_000718"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIP4772583.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            16357..16899
                     /locus_tag="AC1LW8_000719"
     CDS             16357..16899
                     /locus_tag="AC1LW8_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HIP4772584.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            17053..17319
                     /locus_tag="AC1LW8_000720"
     CDS             17053..17319
                     /locus_tag="AC1LW8_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIP4772585.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            17322..19040
                     /gene="ptsP"
                     /locus_tag="AC1LW8_000721"
     CDS             17322..19040
                     /gene="ptsP"
                     /locus_tag="AC1LW8_000721"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIP4772586.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(19277..19510)
                     /gene="nrdH"
                     /locus_tag="AC1LW8_000722"
     CDS             complement(19277..19510)
                     /gene="nrdH"
                     /locus_tag="AC1LW8_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HIP4772587.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            19708..21069
                     /locus_tag="AC1LW8_000723"
     CDS             19708..21069
                     /locus_tag="AC1LW8_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIP4772588.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            21066..22085
                     /locus_tag="AC1LW8_000724"
     CDS             21066..22085
                     /locus_tag="AC1LW8_000724"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIP4772589.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            22218..22880
                     /locus_tag="AC1LW8_000725"
     CDS             22218..22880
                     /locus_tag="AC1LW8_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP4772590.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(23229..24926)
                     /gene="rnjA"
                     /locus_tag="AC1LW8_000726"
     CDS             complement(23229..24926)
                     /gene="rnjA"
                     /locus_tag="AC1LW8_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HIP4772591.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(24926..25144)
                     /locus_tag="AC1LW8_000727"
     CDS             complement(24926..25144)
                     /locus_tag="AC1LW8_000727"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HIP4772592.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(25173..25295)
                     /locus_tag="AC1LW8_000728"
     CDS             complement(25173..25295)
                     /locus_tag="AC1LW8_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772593.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
BASE COUNT         8718 a         3645 c         4754 g         8282 t
ORIGIN      
        1 ctgaaacaga acaattagtt aagcctgtga aaataattcc attagaagta gttgttcgta
       61 atattgctag tggatctatt acaaagcgtt taggttttga aaatggtgaa gtttttagag
      121 aaccacttgt agaatttttc tataaaaatg atgcgttaaa tgatccgttg ataacggatg
      181 accatgttaa attgctcaat atagcatcag atgaagatat tgaaatacta aaatccaaag
      241 cattaaagat taataatgtg ttgaaacaat taatggatgc tatgaattta aaattagtag
      301 attttaaaat cgaatttgga aagactgaga ctggtcaaat tttgttagcg gatgaaatat
      361 ctccagatac atgtcgaatt tgggataaag ctaccaatgc aaactttgat aaagatgtat
      421 atagaaataa cactggatca ctgattgaaa catatcaaat atttttaaac aaattggagg
      481 atttaaaata atgaaaacaa ttgaactaca tatcacatta caaccacaag tattagatac
      541 gcaaggacaa acgcttactc gagctgtaca tgacttaggt tatgcacaag tgaatgatat
      601 tcgtgtagga aaagtattat atatgacagt ggatgaggtt agtgatgaaa aggtacacaa
      661 cattattaca actctaagtg aaaaattgtt tgcaaataca gtgattgaag aatatagcta
      721 taaagtgtta gatgatgaaa aggagaatgc ataaaatgaa atttgcggtt cttgtttttc
      781 caggttcgaa ttgtgataga gacatgttta atgctgctat taaaagtggt gttgaagcgg
      841 aatatgtaga ttatagagaa acatcactaa gtggatttga tggcgtactt attcctggtg
      901 gattttcatt cggggattac ttaagatctg gggcaatggc tagtgtagcg ccgattattt
      961 cggaagttaa acgtcttgca gctgaaggta agccagtatt aggtgtttgt aatgggtttc
     1021 aaattttaac tgaaataggc ttattacctg gtgcattatt gcataacgat tcacatttat
     1081 ttattagtag aaatgaagag ttagaaatag tgaataatca aacggcattt acaaatcttt
     1141 atgaacaagg tgaaaaagtt atatatcctg tagctcacgg tgaaggtcat tattattgta
     1201 ctgatgaaat atatcaacaa ttaaaagcta acaatcaaat tattctgaaa tatgtgaata
     1261 atccgaacgg ttcatatgat gatattgcag gaattgttaa cgaaaaaggc aatgtatgtg
     1321 gcatgatgcc acatcctgaa agagctttag aaacgttgtt aggtactgat agtggtgtga
     1381 aattatttga agcgatggta aaaagttgga gggaacaaca tgtctaaatt tatcgaacca
     1441 agcgttgaag aaattaaact tgaaaaagta tatcaagata tgggattaag tgatcaagaa
     1501 tatgaaaaag tttgcgatat tttaggcaga caacctaact ttacagaaac aggtatcttt
     1561 tctgttatgt ggagtgaaca ttgctcttat aaacattcta aaccgttttt aaagcaattt
     1621 cctacgtcag gtgaccatgt gcttatgggg cctggtgaag gtgcaggggt agtcgatata
     1681 ggtgataatc aagccgtagt atttaaagta gagtctcaca atcatccatc agcaattgaa
     1741 ccatatcaag gggctgctac aggcgttggt ggaatcattc gtgacattgt ctctattggg
     1801 gctagaccta ttaatttgtt aaacagtctt agatttggag aattagataa taaacaaaac
     1861 caaagattac ttaaaggtgt tgtaaagggt atcggaggtt atggtaactg cattggtatt
     1921 ccaacaactg ctggtgaaat cgaatttgat gaacgttatg atggcaatcc acttgttaat
     1981 gcaatgtgtg ttggtgttat caatcacgac atgattcaaa aaggcacagc aaaaggtgta
     2041 ggtaattcgg tcatttatgt tggtttgaaa actggtcgag atggtattca tggtgctact
     2101 tttgcatctg aagaattgac ggaagaaagc gaaagtaaac gaccttctgt acaaatcggt
     2161 gatccatttg taggtaaaaa attaatggaa gcaacacttg aagcaattac atttgatgaa
     2221 ttagttggta ttcaagatat gggtgctgct ggtttaacat cttcatcgtc tgaaatggcg
     2281 gcaaaaggtg gtagtgggtt acatttgaga ttagaacaag tgccaacacg tgagccaggt
     2341 atttctcctt atgaaatgat gctttcagaa actcaagaac gtatgttact agttgttgaa
     2401 aaaggtactg aacaaaaatt cttagattta tttgataagc acgaattgga tagtgctgtt
     2461 ataggtgaag ttacagatac aaatcgtttt gttttaacat atgatgacga agtttatgct
     2521 gacattccag ttgaaccact agctgatgaa gcacctgtat atattttaga aggagaagaa
     2581 aaagattata atacttctaa aaatgattat acacacatcg atgttaaaga tactttcttt
     2641 aaattactta agcatccgac tatagcatct aaacactatt tatatgatca atacgaccaa
     2701 caagttggtg ccaatacgat aattaagcca ggacttcaag catcggtagt acgtgtggaa
     2761 ggcacaaata aggcaattgc ttcaacaatt gatggtgaag cgcgttatgt atataacaat
     2821 ccatatgaag gtggaaagat ggtagtagct gaagcttatc gaaatttaat tgccgtgggt
     2881 gcaacaccat tagcaatgac agattgttta aattatggtt ctcctgaaaa gaaagaaatc
     2941 tatcaacagt tgatagattc aacgaaaggt atggcagaag catgcgacat tcttaagaca
     3001 ccagtagttt ctggtaatgt atctttatat aacgaaacga aaggtacttc tattttccca
     3061 acaccagttg ttggaatggt aggtttgatt gaaaatgtaa attatttaaa tgattttgaa
     3121 cctcaagttg gagataaatt atatttaatc ggtgatacta aggacgactt tggtggtagt
     3181 caacttgaaa agttaattta tggcaaagtt aatcatgaat ttgagtcatt agatttgagt
     3241 tcagaagttg aaaaaggtga atcaatcaag accgctattc gtgaaggact attatcacat
     3301 gttcaaacag ttggtaaagg tggcttactg attaccttag ctaaactaag tgcgcattac
     3361 ggtttaggat taaaatcttc aatagatata acaaatgcac aattgtttag tgagacgcaa
     3421 ggccgatatg ttgtttctgt taaatcaggt aaaactttaa atattgataa tgcaatagaa
     3481 attggacttt taacagatag tgataatttc aaggtaacaa caccatatac agagattagt
     3541 gaaaatgttt cagatattaa acaaatatgg gaaggggcaa ttgctcaatg tttaactact
     3601 caggattaaa cgaagaatgt ggcgtgtttg gtatttggaa tcatcctgaa gcagcgcaac
     3661 taacatatat gggacttcat agtttgcaac atcgtggtca agaaggtgca ggtatagttg
     3721 tttctgatca aaatgaatta aaaggcgagc gaggattagg cttactaact gaagcgatta
     3781 aagatgatca aatggaacga ttaaaaggat atcaacatgc aattggtcac gtccgttatg
     3841 ctacttcagg taataaaggt attgaaaata ttcaaccgtt tctgtatcac ttttatgata
     3901 tgagtgtagg tatttgtcat aatggtaacc tcattaatgc taaatcattg cgtcagaatt
     3961 tagaaaaaca aggtgctatc ttccattcgt cttctgatac tgaagtcatt atgcatttga
     4021 tacgtcgaag taaagctcct acttttgagg aagcgttgaa agaaagtttg cgaaaagtta
     4081 aaggcggttt tacatttgcg attttaacta aagatgcgtt atatggcgca gtagatccaa
     4141 atgctatcag accacttgtt gtaggtaaaa tgaaagatgg gacatacatc cttgcaagtg
     4201 aaacatgtgc aatagatgtg ttaggtgcag aatttgttca agatattcat gcaggtgaat
     4261 atgtcgtgat taacgataaa ggtattacag ttaaatctta tacacatcat acgacaactg
     4321 caatttctgc gatggaatat atttattttg ctagaccaga ctcaacaata gctggtaaaa
     4381 atgtccatgc agtacgtaaa gcttctggta aaaaattagc ccaagaaagc cctgtaaatg
     4441 ctgatatggt catcggtgta cccaattcat cgctatcagc tgcgagtggt tatgctgaag
     4501 aaataggttt gccatatgaa atgggactag ttaaaaatca atatgttgca agaacattta
     4561 ttcaaccaac tcaagaatta cgtgagcaag gtgtgagagt gaagttatct gcggtaaaag
     4621 atatagtaga tgggaaaaat atcattcttg ttgatgattc cattgttcgc ggtacgacaa
     4681 ttcgacgcat tgtgaaaatg ttaaaagatt ctggtgcaaa taaagtgcat gtgcgtatag
     4741 catcaccgga atttatgttc ccaagttttt atggaatcga tgtttcaact acggcagaat
     4801 taatttctgc aagcaaatca cctgaagaaa ttaaagatta tattggcgct gattcattag
     4861 catatctatc tgtagatggg ttaattgaat caattggttt agattatgac gcgccatata
     4921 gtggcttatg tgtagaaagt ttcactggag attatcctgc agggttatat gattatgaag
     4981 caaattataa agcacattta agtcatcgac aaaagcaata tatttctaaa aacaaacact
     5041 tttttgatag cgagggaaat ttaaatgtct aaagcatatg aacaatctgg tgtaaatatt
     5101 catgctggtt atgaagctgt agaaagaatg tctagtcatg ttaaacgtac gatgcgtaaa
     5161 gaagttatcg gtggtttagg tggattcggt gctacatttg atttatcaca attaaatatg
     5221 acagcgccag ttttagtttc tggaacagac ggagtaggta cgaaattaaa actagctatc
     5281 gactatggga aacatgattc gataggtatc gatgcagtcg caatgtgtgt taatgatatt
     5341 ttaacgacag gtgcagaacc attgtatttt ttagattaca tcgctacaaa taaagttgtt
     5401 cctgaagtta ttgaacaaat tgttaaaggt attagtgatg catgcgttga aacgaatact
     5461 gcacttatcg gtggagagac tgctgaaatg ggtgaaatgt atcacgaagg tgaatatgat
     5521 gtagccggat ttgcagttgg agcagttgaa aaggatgact atgtagatag ttcagaagtg
     5581 aaagagggac aagttgttat agggcttgcg tcaagtggca ttcattcaaa tggatatagt
     5641 ttagtgcgca aattaattaa tgaatcaggc attgatttgg catcaaactt cgataatcgt
     5701 ccatttatag atgtcttttt agaaccaact aaattatatg tcaaacctgt acttgctttg
     5761 aaaaaagaag tttctattaa ggcaatgaat catattactg gtggaggttt ttatgaaaat
     5821 attccacgtg cattgccagc cggatatgct gctagaattg atactacatc atttccaacg
     5881 ccaaaaatat ttgattggtt acaacaacaa ggcaatatag acacaaatga aatgtataac
     5941 atttttaaca tgggtattgg ctatacggtt atcgttgatg aaaaagatgc atcacgcgct
     6001 ttgaagattt tagcagaaca aaatgtggaa gcctatcaaa ttggtcatat tatgaaaaat
     6061 gagtcaactg caattgaatt gttgggggta taacaatggt taaaattgcg atttttgcat
     6121 caggttcagg aagtaacttt gaaaatatag ttgagcatgt tgaatcagga aaacttgaaa
     6181 atattgaagt tacggcgcta tatacggatc atcaaaatgc gttttgtata gatagagcaa
     6241 aaaagcacga tattcctgtt tatattaatg aaccaaaaca atttgattca aaagcagcgt
     6301 atgaacaaca tttagtatca ctattaaatg aagataaggt agagtggatt attttagctg
     6361 gctacatgcg tctaattggt ccagatttat tagcttcatt tgaaggtaaa atattgaata
     6421 tacatccatc tctattgccg aaatataagg ggattgacgc aataggccaa gcatatcata
     6481 gtggcgatac tattactggt tcgacagtac attatgttga ttgtggtatg gatacgggag
     6541 aaattattga acagagacaa tgtgatatta gaccggacga ttcaaaagaa caattagaag
     6601 agaaagtaaa aaaattggaa tatgagttat atccaagtgt tattgctaaa attgtaaaat
     6661 aaggagtatg tatgtgatga agaaagctat tttgagcgta tcaaataaaa caggtattgt
     6721 agagtttgct aaagcgttaa cgcaattaaa ttatgaatta tattcaacag gtggtactaa
     6781 acgtatatta gatgaagcaa atgtaccagt tcgttctgtt tcagacttaa cacattttcc
     6841 agaaataatg gatggccgtg ttaaaacatt acatccggct gttcatggtg gcattttagc
     6901 tgatcgaaat aaaccgcagc atttaaatga attatcagaa caacatatag atttaattga
     6961 tatggtagta gttaatttat atccattcca acaaactgtt gcaaaccctg atgtgacgat
     7021 ggacgaagca attgaaaata ttgatattgg tggtccaaca atgttacgtg ctgcagctaa
     7081 aaactataaa catgtaacaa caattgtaca tccggcagat tatcaagaag tattgacgcg
     7141 attaagaaac gattcgttag atgagtcata tagacaatca ttaatgatta aagtttttga
     7201 gcatactgca gaatatgatg aagcgattgt acgtttcttt aaaggggata aagaaacttt
     7261 aagatatgga gaaaatccac aacaatcagc gtattttgtg agaacttcga atgctaagca
     7321 cacgattgca ggcgctaaac aattacatgg gaaacaatta agctataaca atattaaaga
     7381 tgcagatgct acactagctt tagttaaaaa gtttgataca cctgctacag ttgcggttaa
     7441 acacatgaat ccatgtggtg ttggtatcgg tgacacgata gaacaagcat ttcaacatgc
     7501 gtatgaagcg gatagtcaat caatatttgg tggaattgtt gcattaaacc gagctgtaac
     7561 acctgagtta gcagagcaat tgcatagtat ctttttggaa gtcattattg caccaaaatt
     7621 tacagatgaa gcattagata ttttaaaaca aaagaagaat gtaagattat tagaaattga
     7681 tatgactata gacagtaacg aagaagagtt tgtttcagta tctggcggat atttagttca
     7741 agataaagac aattatgtcg tgccaaaaga agaaatgaaa gttgttacag aagtagcacc
     7801 tactgatgaa caatgggaag caatgttatt aggatggaaa gttgtaccat cagtaaaaag
     7861 taatgcaatt attttaagta ataataaaca aactgtaggt ataggtgctg gacaaatgaa
     7921 tcgtgtcggt gctgctaaaa ttgcgttaga gagagctatt gaaatcaatg atcatgtagc
     7981 gttagtatct gatggatttt tccctatggg agatacagtt gaacttgcag cacaacatgg
     8041 tataaaggca attatccaac cgggtggttc gattaaagat caagattcaa ttgatatggc
     8101 taataaacat ggtattgcaa tggtagtcac aggcactcga cattttaaac actaatttta
     8161 aaggggcgta acataaatga atgtattagt aattggtgct ggtggacgag aacatgcact
     8221 tgcatataaa cttaatcaat cgaatctagt taaacaagtg tttgccattc caggtaatga
     8281 ggcaatgaca cctatagctg aagtacacac tgaaatttca gaatctgatc atcaagcgat
     8341 attagatttt gctaaacggc aaaatgttga ttgggtagtt ataggtccag aacagccgct
     8401 aattgatgga ttagcagaca ttttacgagc gaatggtttc aaagtgtttg gtccaaataa
     8461 gcaagcagct caaatcgaag gctcaaaatt atttgctaaa aagataatgg aaaaatataa
     8521 tattccaact gctgattata aagaagttga gcgaaaaaag gatgctttaa catatattga
     8581 aaactgtgaa ttgcccgttg ttgtcaagaa agatgggtta gctgctggga aaggcgttat
     8641 tattgcagat actattgcag cagccagaag tgctattgag attatgtatg gtgatgaaga
     8701 agaaggtact gttgtatttg aaacgttttt agaaggtgaa gagttctcgc taatgacatt
     8761 tgttaatggt gatttagcag tacctttcga ctgtattgca caagatcata aacgcgcatt
     8821 tgatcatgat gaaggaccaa atactggtgg tatgggggct tattgtccag taccacatat
     8881 tagtgacgat gttttaaaac ttacaaatga aacaattgca caacccattg caaaggcaat
     8941 gcttaatgaa ggttatcaat tcttcggtgt attatacatt ggtgctattt taactaaaaa
     9001 tggtccaaaa gtaatagaat ttaatgcccg ttttggtgat cctgaagctc aagtattatt
     9061 aagtcgcatg gaaagtgatt taatgcagca tattattgat ttagatgaag gaaaacgtac
     9121 tgaattcaaa tggaaaaatg aatctattgt aggggtcatg ttggcatcaa aaggatatcc
     9181 tgatgcatat gaaaaagggc ataaagtaag tggctttgat ttaaatgaaa actattttgt
     9241 tagtggatta aagaagcaag gtgatacctt tgttacttca ggtggtagag ttatacttgc
     9301 catcggaaaa ggtgacaatg tacaagatgc acagcgagac gcatacgaaa aagtatcaca
     9361 aatacaaagt gaccatttat tctatcgtca tgacattgcg aataaagcac tacaacttaa
     9421 ataagtaaat ttaaaatact aagattagct atgaacgaat ctataacgat agattttttc
     9481 atagcttttt tagttgtaga gtctaggaca ttgatttctg taccaaattt gtgattatgc
     9541 atatgtaata caaaagaggc gccacaacat gtttggatga acaaaataac atgtttgtgg
     9601 cacctctttt gtttagtatg gaataaatgg ttttcttttt ctatacaatg aatttctaat
     9661 ttagtatcta tacaattatg gataaaattt aacctacacg accaagacga acatcatcta
     9721 tgcccgtgat gggtaaggtg attgaacaat aatatgccaa agtaataatg gcaattaaaa
     9781 ctataataaa gattatatct ttatatgaga aaggtacgtt gtaatagtaa gtacgaggac
     9841 catctctaaa tcctttggac tccatcgcaa ctgataattg atgtgccttt ctaatatttt
     9901 ggcttaatag aggtataatt aaatgcttaa atcgctttaa ccctctataa tttgctgcgt
     9961 ctatcatctg atagcgcatt tttaaagatc tgcgaagctg tattaaagaa ctaatcatta
    10021 aaggtatcat acgaatggca gccatgaatg cataagcaac ttttgattta acctttaaat
    10081 gttgcattaa actataaaat atcatgacaa cctgagatgt aagtgcgatt aagataccga
    10141 aaaatgaaat agcaatggtt cttaatgata catgtaaacc acgaactaaa ctttctgttg
    10201 taatatggat aaatccgaat ttcaaaattg tatggctacc attcccgtat aaaatcatga
    10261 acagggaaga gagtaatgca aagccaatac ttatagttat aaaaattgct gtaattttaa
    10321 actgagtacc attaaacatc aataagaaaa ctaacattaa gatagtgata taaagcataa
    10381 aatcgaaatt atgcacaaat ataataaaga aaaatagtat aattccaaga aatagtttcg
    10441 ttataatgtt gacatcatca acaaatgatt gccgaacttt ccattgctca tacattcgta
    10501 tcaccatcac aatctagtaa cgcaccatct gaaattttaa gtcttcttga tggataacgt
    10561 tcaattattt catcgtcatg tgtaaccatg acaatacttt gtcccaaatt aattcgcttt
    10621 tggaaaagtt tgatcaactg gaatgtatta tggctatcaa gtccaaatgt cggttcatct
    10681 aaaaagataa tatcagcttt agaacttagt gcggtagcta cgctaaggcg tcgtttttga
    10741 ccaatagaca actcataagg atgttgatct tttacatttt gtaaatctaa aagttttaaa
    10801 agttgtatcg tttcatcatc actttgatct ttagaaaggt gattaaaatg aatgttaatt
    10861 tcatcataaa ccgaatttgt tataaattgt aattctgggt tttgataaac taggtacatg
    10921 tgttttgctg catgtttaat ttttgttaaa cgctgatttt caaaataaac atcaccttga
    10981 tatttaatca attgcataat tgattcaagc aaggttgttt taccactacc atttgcccct
    11041 gtaattgtaa tccactcacc tagaccaatt tctaaatctg agaatgagag caatgttgat
    11101 ttaccgcgaa taatacgtcc atttttaaat tgtaataagt gtgagtttgt tgttggaaag
    11161 tcaacacgac ttggtgcgaa ttcccatgca cgtggatgcc acacaccata ttcactgagt
    11221 aaatgaacat acttctgtaa tatgatttca ggacattcat cggcaatgat atttccgtta
    11281 taatccatca aaatgacgcg gtcgacatga ttccagatgt gtttaacttt atgttcaacg
    11341 attacaacag tttgatcttc ccaaagttca attagtttag tccataaatc ttctgttgct
    11401 tgaacatcta acattgctgt cggttcatct aaaaacaatg tttttgattg ttgaagaatg
    11461 gtttcaacaa ttgccaattt ctgtttcatc ccgccactta aatctttgat atacgtttca
    11521 ggggtaacat ttaaattgac catatttaaa gcattgataa ttaacgcatc catgtcttca
    11581 cgtggtactt gtctattttc taaaacgaat gcaagttctt cgtatacttt tggcatacaa
    11641 aactggctat cagggtcttg gaaaataacg ccacttaatg ggtcaacgat tagttcatca
    11701 tatttcatag gtaattcaat taaattagga acaataccac ttaatacatt cagaagtgta
    11761 cttttaccgc aaccagaagg accgagtaaa agtacttttt ctttgtcttg aatagtgata
    11821 tttaaatgat cgaaaatttt acgctgacca cttggatatt ttaatcgtaa atcacttact
    11881 tttaacactc tgaatgctcc tcttataagt tgtcgtaatc ttctttagca gctggtctaa
    11941 ataattttgt tacgcctgtc ttatctaaag cttttactaa aaggtaagat aggacgccgg
    12001 cgactactgc accactaatt aatctaaata cgatgaataa tgttaagttc caacctgcaa
    12061 cttcatttaa ataaccatag aaataatcta tagggaaagc cgcgattgct gtacaaaaac
    12121 ctgctaacat agctaccata actgaacgag attgatattt aaaaattgca aagacaagtt
    12181 cacacgctaa accttgtatg aaagcgtaaa cgattgtcgg aatatcgaaa cgacccataa
    12241 tgatagtttc gccggcacct gcagcaaatt cagccagtaa agcaatacct ggttttggaa
    12301 taattagata gcagacaatc gctgccatga accaaacccc gtttgttaat tgttcgaggt
    12361 gaaggcctgt agcttgcaca ccattgtaaa caaaccacca taaattgtaa ataactgcga
    12421 atactactga aataagtacg gttactagta tttcagatag ctttaaacct tttgacattt
    12481 ttacatcctc ctaataaaaa aacgcacaac catccatagg aaagttatgc gttcacaata
    12541 tatattagta aaacatatgt atagtaacac tttcctacgc tagttcaagc tagatcaggt
    12601 tcaaagggtt tgagggcaag cctcatctca gtataaaaca cccctagtgt gtgcgattta
    12661 tttaattaat tatactgtaa gacgtttgta aacttatgtc aataggttgt cttcatgaaa
    12721 ttttgtttaa ttcgatttaa aatttataat attagcattg gatttaaatt gaagatgtag
    12781 taggaatgtt agtaattaaa gatataaaaa tatgtgacat gtaataagat tgagctgata
    12841 aatgaagagg gatacttatc aatcatacct ctttaacaac agtgaagaac ccgtgcataa
    12901 tggcttacga attatagttt gtagggtaaa agagggatac atgcgccgag cacatgcaaa
    12961 aaagccccta acaactaaaa gttgtaaggt aaaagaggga tacatgcgcc gagcacatgc
    13021 aaaaaagccc ctaacaacta aaagttgtaa ggaagaagag ggacacatgc gccgagcaca
    13081 tgcataaaat cccctaacaa ctaaaagttg taaggggatt taaattaatt tagtgtatct
    13141 tggatatctt gttttgtttg attaatatct tctgtttttt cttctttttt atcttttaat
    13201 ttttcttcaa cttctttagc tttttctgct gcttttttat tttgattttc attagacatg
    13261 attaattcct cccaaattgg ataattattt atatataaat cttacccggt tgtactttcg
    13321 ttaaactttt ctaagtctat agcactattt attcatttat ctaaagacaa caacattaga
    13381 ttaatatata atgattttga ggtgaacata atgtcttttc ttaggaaaca cgccgaaatt
    13441 atttttagct atttaatcgg tatcgtttca ctcttcactg gtctcattat tttaattaac
    13501 ttgccattaa ttaaacaatt aaatggtggt aaaaaagttg atacacatgt tcataatgtg
    13561 tgggaatttc tgaatgcatt tttcagtgaa attattaaag taatgagtcg atttataggt
    13621 aatttcccta tagttagtgc aattgtgata attatattcg gtattttagt tatgttgatt
    13681 ggtcatacat tacttagaac tattaagtat gactatgata tttctatctt tttcttagtt
    13741 atcggtatca tgtactttat tattactctt atattaatga ctcaagttta tggattcttt
    13801 gcagtgattt tcattattcc atttacaatt catataggat atatcgtcta taaagatgaa
    13861 ttgaatcagg aaaatgtaaa aaatcatttc atgtggataa ttgtgagtta tggtataagt
    13921 tacttaatta cacaaattgc attgtatggc agaattgatg ctaatgaaat agagtcaatt
    13981 gatatcttaa gtgtcaatgc tttctttata attatgtggt tacttggtca aatggctatt
    14041 tggaatttct tgttcttgcg ccgagcttta cctttaacaa agcaagaatt aggtgaagag
    14101 gagccagaat tatcaagaac aagtaaaggg aatgtcacga atcaaactaa aattcacttg
    14161 aaacaactcc aagataagac tacagaatat gcacgtaaga caagaagaag tgtcgattta
    14221 gataaaatta gagctaaaag agataaattc aaaaagaaag ttaatgatat tatcgatatt
    14281 caagaagacg atattcctga ttggatgaga aaaccgaaat gggttaaacc aatgtatgtc
    14341 gaactatttt gtggtgtcgt catcttttta ttcacatttt tagaatttaa taatcgtaat
    14401 gcattatttg tatctggtga ttggaaatta tcacagacac aatatgttat tgaatgggtt
    14461 acattactaa ttctgttatt cattattatc gcatatatcg ctacaacgtt aactttccac
    14521 ttgaaaggta agttttatta tttacaacta tttatgggga gcattttatt ctttaaattg
    14581 ttaacggaat tcataaatat aatgattcat ggactactac tttcagtgtt cattacacca
    14641 acattactat taatgttatt ggcaatcatc atttcttatt cgttacaatt acgagagcga
    14701 ccataattaa aagcattata aaagtactat ctattaaaca ttttgatgtg tacgctataa
    14761 gttagatata tctctaactt atttagatac aggtcaatga agtttatgga tagtactttt
    14821 tttgtaacta gatttgattg attcaggtga tgtgaattaa gtattgaaaa ttgtatacaa
    14881 agttttagtg caaataaaat agttgaaaag ttatccattt gtaaaatcaa gaaaactatt
    14941 aaatagttga atcgacattt ggaaattgcg aaacgatata tagtatttcc tttgtagaaa
    15001 tttaacatat atcattcgaa ttactaattt gttaaaatca acattaagat tagaagtaga
    15061 tgatattgaa atttggcaaa caatttaatc tatataaaac tacaacgaaa cacagaaagg
    15121 aagttgtcag atgaaaatag caactctgaa caaaggcaaa gaaacaaaat attttaatgg
    15181 atatccttta attgaagaag aggatatcta ttcacaagat catttaaaag aaggagatat
    15241 ttttcaaatt gtgactgata aatcacaata tgttgcaacg gcttatgttg gtcgccaaca
    15301 taaaggatta ggttgggttc taacatacga taaagctcaa gaaatcaaca cagctttctt
    15361 tgtgaaattg tttaatactg cattagcaga acgtgattat tattttaata tagatggaac
    15421 aaatgctttt agattattta atgctgaagg tgatggtgtt gggggattaa caatcgacaa
    15481 ttacgatggt catttgttga ttcaatggta ctcaaaaggt atttataaat ttaaatatgc
    15541 cattcttgaa gcggttagaa aagtatttga ttataaatct atttacgaaa aagtaagatt
    15601 taaagacagc gaatatagtg gtggttttgt tgaaggagat gcacctgagt ttccaattgt
    15661 tatcgaagaa aacttcacat tttataatgt agaccttgaa gatggtttga tgacaggtat
    15721 ctttttagat caaaaagaag tgcgcaagaa attaagggat caatatgcca aagaacgcca
    15781 tgttttaaac ttatttagtt atacaggtgc tttttctgta atagcagcaa gtgaggcatc
    15841 ttcaacaaca agtgtagatt tggctaatcg ttctcgtagt ttaactgaag aaaattttgg
    15901 attaaatgct attgatccta aatcccaata tatttatgtc atggacactt ttgatttcta
    15961 taaatatgct gcacgacatg gacatagtta tgacacgatc gtgattgatc cacctagctt
    16021 tgcgcgtaac aaaaaacgta cattttcagt gcaaaaagat tatgacaaat taattaatgg
    16081 cgccttaaat atcttatcat ctgaaggaac attattgtta tgtacaaacg caagtgtata
    16141 tccattaaag caatttaaaa atactattaa aaagacgctt gaagagagtg gcgttgatta
    16201 tgaattaact gaagttatgg gattaccaaa agattttaaa acgcatccac attataagcc
    16261 atctaaatat ttaaaagctg tttttgtaaa tattagacat taatttgata aaaaggggta
    16321 tagtaactta ttgagaaaaa gaagggtgat aatattatgg gattcaaaaa caatttaaca
    16381 tcaaatttaa caaataaaat tggtaattca gtctttaaaa tagaaaatgt tgacggaaaa
    16441 ggtgcaatgc caacgacgat tcaagaattg agagaaagac gacaacgtgc tgaagcaatt
    16501 gtaaagagaa aatctttaat gtcatcaaca atgagcgttg ttccaattcc gggtttagat
    16561 tttggtgttg atttaaaatt aatgaaagat attatcgaag atgtaaataa aatatacgga
    16621 ttagatcata agcaagttaa tagccttggg gatgatgtga aagaaagaat tatgtctgca
    16681 gcagcaattc aaggtagtca atttattggt aaaagaattt caagtgcatt tttaaaaata
    16741 gttattagag atgtagctaa acgtactgct gcaaaacaaa caaaatggtt tcctgttgta
    16801 ggacaagctg tgtctgcatc tattagttac tattttatga ataaaattgg aaaagatcac
    16861 attcaaaaat gcgaaaatgt tattaaaaat gtcatgtagg tgctataata gttttgcaat
    16921 ttgcaaattt tactgaaacc ggttttaaac gaattgaatt taaagcatgg ttttggtaaa
    16981 gttaatgtat aaaactaagt tagtattgta ataatattga agattctaac tatacgaagg
    17041 agaaatgtaa ttatggaaca aaattcatat gtaatcatcg acgagactgg tattcacgct
    17101 agaccagcaa caatgttagt acaaacagct tcaaaattcg attctgatat tcaattagaa
    17161 tataacggta agaaagtaaa cttaaaatca atcatgggtg ttatgagcct tggtgttggt
    17221 aaagatgctg aaattacaat ttatgctgac ggtagtgatg aatctgacgc cattcaagca
    17281 atcagtgacg tcttatcaaa agaaggattg actaaataat catgtctaaa ttaattaaag
    17341 gtattgccgc atctgatggt gtcgcaattg ctaaagctta tttattagtt gagccagact
    17401 taacattcga caaaaatgaa aaagtcactg atgttgaagg agaagttgca aagttcaata
    17461 gcgctatcga agcttctaaa gttgagttaa ctaaaattag aaataatgca gaggttcaac
    17521 taggtgctga taaagctgct atctttgatg cacatttatt agttttagat gaccctgaat
    17581 taattcaacc aatccaagat aagattaaaa atgaaaacgc taatgctgct acagcattaa
    17641 cggatgtaac aacacaattt gttacaattt ttgaatctat ggataacgaa tacatgaaag
    17701 aacgtgcggc tgatattcgc gacgtttcta aacgtgtgtt atcacatatt ttaggtgtag
    17761 aattaccgaa tccgagtatg attgatgaaa gcgttgttat tgtagggaat gacttaacgc
    17821 catctgatac tgctcaatta aataaagaat tcgtacaagg ttttgctaca aacattggcg
    17881 gaagaacaag tcactctgca attatgagtc gttctttaga aattccagca attgttggta
    17941 caaaatcaat tactcaagaa gttaaacaag gcgacatgat tatcgtagat ggattaaatg
    18001 gtgatgtaat cgttaatcca actgaagatg agttaatcgc ttatcaagat aaacgtgagc
    18061 gttattttgc tgacaagaaa gaattacaaa aactacgtga tgctgatact gttacagttg
    18121 atggtgttca cgcagagctt gctgcaaata ttggtacacc taatgatttg ccaggtgtta
    18181 ttgaaaatgg tgcacaaggt atcggcttat atagaactga gtttttatat atgggtcgtg
    18241 accaaatgcc tacagaagaa gaacaatttg aagcttataa agaagtatta gaagcaatgg
    18301 acggtaaacg tgttgttgta cgtactttag atataggtgg agataaagaa ttatcatact
    18361 taaacttgcc tgaagaaatg aatccattct taggttaccg tgcgattcgt ttatgccttg
    18421 cgcaacaaga tattttcaga ccacagctac gtgcattatt acgtgcatca gtttatggta
    18481 agttaaatat catgttccca atggttgcaa caattaacga atttagagaa gctaaagcta
    18541 tattattaga agaaaaagaa aaccttaaaa atgaaggtca tgacatttcg gatgatatag
    18601 aattaggaat catggtagag atacctgcaa cagcagcatt agctgatgtc tttgctaaag
    18661 aagtagattt cttcagtatc ggtacaaatg atttaattca atacacatta gctgctgacc
    18721 gtatgtcaga gcgtgtatca tatctatacc aaccatataa cccttcaatc ttacgtttag
    18781 ttaaacaagt tattgaagcg tcacataaag aaggtaaatg gacaggtatg tgtggtgaaa
    18841 tggctggaga tgaaacagct attccattat tgcttggttt aggtttagat gagttctcta
    18901 tgagtgcaac gtctattctg aaagcaagaa gacaaattaa tggtttaagt aaaaatgaaa
    18961 tgactgaact tgctaaccgt gcagtcgact gtgcaacgca agaagaagtt attgaattag
    19021 ttaacaacta cgtaaaataa ttaattcttt cgaaagacag aattataaat aaacccgaag
    19081 tattcattgt gagtaattat gaatacttcg ggttcttttt tatgatttat gttaatttca
    19141 atggaatgtt gttgctaact aattaaacaa ctatgtgtca ttaacaaaat gttgttcttt
    19201 aattaatgtt agttaattta aaaatcgata tttaatttat gatagctata aatgatgaag
    19261 ttgatttaga tgaaaattaa tcttggatat ttaatacttt gttgatttca tcaagatcaa
    19321 catggtacat tggattacca ttcaacaaaa taaacggagt tgaaaaagca tcaaaatcta
    19381 tcatttcgtt tcgatattgt tgattgttga tatttctctc ttcaaaatca atttgatgct
    19441 cattaagata attttttaca aatgtacaag gtgggcaatc attctgcgta taaacgatta
    19501 tttctgacat gtatcatgct ccttcgtgtt ttattctaat gtaatataca caattataca
    19561 ttgaaattca aatttgtgaa cacattgtga actgacataa attatacaca atttcatagc
    19621 gcataatgtt ttaaaaatac acattatagc aattataatt aagttcattg tgttgtcatc
    19681 aaaaaaagaa aaaggtgatg tttttaaatg gatacagttg aaatcagtcg gtttttgaca
    19741 gctatgactt tagcagttca tatcattttt gcaacgattg gtgttggtat gcctttaatg
    19801 ttcgcaattg cagaattttt aggtattcgc aaaaatgatc ttcaatatat agctatggcc
    19861 aaaagatggg ctaaagctta tacaattact gtagcagtgg gagttgttac aggtacaatt
    19921 ataggacttc aattatcatt gatttggcct acatttatgg aaatgggtgg acacgttatt
    19981 gcacttccat tatttatgga aacatttgcg ttcttctttg aagctatttt cttaagtata
    20041 tatttatata cttgggatcg ttttaaaaat aaatggacac atttcttaat tagtatacca
    20101 gtaattattg gtggctcttt ctcagcattc ttcattactt cagtgaattc atttatgaat
    20161 acgcctgcag gttttgagtt gaagaatgga aagatggtca atgttcaacc tatagaagcg
    20221 atgtttaacc catcgtttat agttcgatca tttcacgtaa ttactacagc cggtatgacg
    20281 atggcatttg ttattgcatc aatagcagct tttaaattat tgcgtaatcg tcaacctaaa
    20341 gatactgtct accataagaa agctttgaaa atgtctatga tagttggatt cttttcaaca
    20401 ttactttcta tgttggcagg ggatttatct gcaaaatttt tgcataaatt ccaacctgaa
    20461 aaattagcag cttatgaatg gcatttcgat acatcttccc atgctaaatt attattattt
    20521 ggtgtgttag atgaaaagac tcagcaagtt aaaggtgcga ttgaattacc tggactattg
    20581 agtttcttag cagataatag tgtcaaaact aaagtgcaag ggttaaatga ttttccaaaa
    20641 agtttacatc cgcctatgat tgtccattat ttctttgatt taatggtaac gatgggaatt
    20701 ttatgttttg tcatttcagg tgtttatgtt ttaactttaa tgtttaaaaa gcttagaaag
    20761 ttttctactc ataaatggat gctttacgga atattattaa caggaccagc ttcaatgcta
    20821 gctatagaat ttggatggtt cttaacagag atgggtagac agccttggat tgttcgtggt
    20881 tatatgcgcg tggcagaagc agcaacacaa gcaggcggaa taaccttcgt tacaatttta
    20941 tttggcatat tgtacatcat tttaatgtat acatgtgcat acgtattaat tcgtatgttt
    21001 aaaaataaac cggcgtatga agatgtaaat cgtttagcca agaagcaagg aggagaaata
    21061 gaaaaatgat ttatgcattt ataggtataa cagtgttatg gttgttttta ttttgctata
    21121 tcattattgc ttctatagat tttggggcag gtttcttcgc attgcattca aagttaactg
    21181 gtgatgaaaa gaaaattaat cacttaattt cacgttattt aaacccagtt tgggaagtta
    21241 cgaatgtatt ctttgttttc ttcttcgtag gattcgtagg tttctttcca gagtcaatca
    21301 aatatttagg cacggtattg ttaataccag gttcaatagc actgattatg atatcgttga
    21361 gaaacagttt ttatgcattt gaaaattatg gtcaagatac aaaattagca tggatgatca
    21421 tgtatggggt aagtggatta ttaattccag cttcattatc tactgcttta actattacag
    21481 aaggtggcta tattaatgtt cgaaacaatg ttatcgatct agattgggtg cagttactat
    21541 taagcccatt tgcttggtct gtagtattct tggcaattat ttcagtttta tatatttcat
    21601 caggattttt gacatattat gctaaaaaag caaatgacga accagcatat aatttaacaa
    21661 gacaatggca catattttta gggccgccga tgattatcat ttgtttattc gtatttctat
    21721 cattacgcat tcaaaattct gaacattttt attcagctgt ttttgactat tggtggatgt
    21781 ttgctataag tttcttattc tttgcattag cttcattgtt aacattcttt aagaaaaaac
    21841 atggtttggc ttttgtattt gttattttac aaatgatgtt cgcgttcttt ggctatggaa
    21901 ttagtaaatt gccatacctt ttatatccgt ttgtaaaaat tacagatgca tatgttaatc
    21961 cagaaatggg ctggacatta gtgattgtct ttattttagg tttactttta ttacttccat
    22021 cgttaatatt attattaaga ttatttgttt tcgacaaaga atatgttgaa ggaaagaaat
    22081 cataagttga gacgataccc caactttcat ttacttgcat tactgcattt agataattta
    22141 gttggggtac tcatttataa taattttaaa tttatatttt caatgaattg atgaaaatca
    22201 tttaatggag ggcgagtatg ggtaaagaat atgtagtcat cggtctaggt cgttttggag
    22261 gtagtatcgt tcgtgagttg aatgcattag acatggatgt aatggccatc gaccatgatg
    22321 aaaatagagt gaatgaatat agtgatatcg ctactcatgc ggttgttgca gacacaacag
    22381 atgaagcagt tatgaaaagt ttaggtatcc gtaactttga tcatgtcatt gtggcaattg
    22441 gtgaaaatat tcaatcaagt acgttgacga ctttaatttt aaaagagtta ggtgtaaaaa
    22501 aagtaactgc taaagcacaa aatgattatc atgcaaagat tttaaataaa attggagcag
    22561 atacggttgt gcaccctgag cgtgatatgg gtagacgtat tgcgcataat gttgcgagtg
    22621 caagtgtact tgattatctt gagttggcag acgagcattc tattgtagaa ttgaaagcaa
    22681 ctgaaaagat ggcggggcag tctatcattg atttagatat aagagcacaa tatggaatta
    22741 acattattgc aattaaacga ggcaaagagt tcatcatttc accaaatcca aatattaatt
    22801 tagaaatagg tgatatttta atcatgattg gacatgataa tgatttaaat cgctttgaaa
    22861 aaaatattgc gacgagataa tcgggagtgg gacagaaatg atattttctc aaaatttatt
    22921 tcgtcgtccc accccaactt acattgtctg tagaaattgg gaatccaatt tctctttgtt
    22981 ggggcccaca ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctatgttgg
    23041 ggcccctgac tagaattgaa aaaagcttgt tacaagcgca ttttcgttca gtcaactact
    23101 gccaatataa ctttgtagag cattgaacat tgatttatgt ctcaagctca tcatatataa
    23161 agaaaaagct actaacaatg ggtggtgagt ttaattaaaa cttcacttca aagttagtag
    23221 ctttttgatt atttattgtt tgattctttt tgttcgttta ccttcataat gactggtaaa
    23281 atcattggtt ttctagctgt tttttcaaat aaataaggtt gtaatgtttc aatgatagaa
    23341 gatttaatct gatgccattg aatatcttta ttttgattta acttactaat aacatcagtt
    23401 ttgattttgc gttgtgcatc ataaattaat tgacctgatt ccctcatata tacaaatcct
    23461 cgagaaataa tgtctggacc agaaagtaat ttatttgtat taaaatcaat actaacaaca
    23521 acgataacta aaccttcttc agataatagc ttacggtctc ttattacaac attaccgata
    23581 tcaccgatac cactaccatc aacaagtaca ttaccagatg gaatgcgacc agctttacgt
    23641 gctgaatcgt gtgttaaagc taagacatct ccaatatcaa agatgaagac attatcttct
    23701 tcaacgccgc attcaacacc agtctcacca tgtgctttta acatacggta ttcaccatga
    23761 ataggtaaga aatatttcgg cttgattaat cgaagcatta attgttgatc accttgagaa
    23821 ccatgccctg aagtatggat gttagaaatc ttgctatgga taacatctgc accagcttta
    23881 tacaaggaat taatagttct gttaatactt tttgtattac ctgggatagg tgatgaacta
    23941 aatacaacgg tatcttcagg tataatttta atttgcttat gagtaccatt agcaattcta
    24001 gataatgctg ccattggttc accttgtgaa ccagtacata gtatcaataa ctcatgcttc
    24061 ggtacggtat taattttatt aggttcaata aatgtttcag gtggtgcttt aatataacca
    24121 agttccatac cgattttaat attgttttcc atcgaacgac cgaacgtaac aattttacgg
    24181 ttatttttga tagcagcttc aactgcttgt tgaactcggt aaatattaga agcgaaggta
    24241 gcaaatataa tacgaccttt acaattacgg aagatcttat ctacgttttg accaacttca
    24301 cgttcgctta aagtaaaatc aggcacaagt gaatttgttg agtctgaaag taaacataga
    24361 acgccttctt cgcctaattg agccatttta gcaatgtttg ctggtttgcc tacaggtgta
    24421 aaatcaaatt taaagtcacc ggtatgaact acttttcctt caggtgtatc tacgatgacg
    24481 ccataagttt caggaatact atgcgtagtt aagtagaaag aaatcgtaaa gtgcttagat
    24541 ttaatcacac tgtcctcatt gatttcattt agtttagcag tacgtaataa atgatgttct
    24601 tcaagtttat tacggattaa acctaatgct aaaggaccac cataaatagg tatattaagt
    24661 tgttttaata ggaagggcac accgcctata tggtcttcgt gaccatgtgt tataaatagg
    24721 ccaacaattt tatcttggtt ttgaactaga tatgtgtagt caggtataac ataatcaatc
    24781 cctaataagt tatcatctgg gaatttgata cctgcatcga taatgacaat ttcgtcttta
    24841 tactcaactg cataagtatt tttaccgatt tcacctagac ctccaagtgc atatacacct
    24901 acttcatttg gatgtaattg tttcattatt tagcaatctc cacattaaag tgttctgagt
    24961 tttctttttc gtaatctaaa tgtgcgccct ctaatttagt gataaattcg atattaaaat
    25021 tacgatcttt caagtaacga cgtacttgtt cttctgtttg agcttcaaca taaagtgatt
    25081 gtgtattttc acgcacaatt acctcgtctc tgttatgttg ataaaaaact ttaaatactg
    25141 ccatgttaaa aattcctcct aagaatgttt gtttaattta ttgttaaccc tagtaaaatc
    25201 gtattggagt atatatcgat aaattcattc caatcatctc tataatgtaa cttaataacg
    25261 atttggttaa taactaggtt catcatgtcg ttcattttaa aaaattagtg aaataacact
    25321 aaaatttcag ttaaatctta ttttacatga tgaatgataa taaataaagc agtttatctc
    25381 actagaaatc gaagtattt
//
LOCUS       DBNENW010000022        25094 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNENW010000022 DBNENW010000000
VERSION     DBNENW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.022"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            154..1050
                     /gene="xerC"
                     /locus_tag="AC1LW8_000729"
     CDS             154..1050
                     /gene="xerC"
                     /locus_tag="AC1LW8_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIP4772594.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1047..1592
                     /gene="hslV"
                     /locus_tag="AC1LW8_000730"
     CDS             1047..1592
                     /gene="hslV"
                     /locus_tag="AC1LW8_000730"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIP4772595.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1658..3061
                     /gene="hslU"
                     /locus_tag="AC1LW8_000731"
     CDS             1658..3061
                     /gene="hslU"
                     /locus_tag="AC1LW8_000731"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIP4772596.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3086..3859
                     /gene="codY"
                     /locus_tag="AC1LW8_000732"
     CDS             3086..3859
                     /gene="codY"
                     /locus_tag="AC1LW8_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIP4772597.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3910..4002
                     /locus_tag="AC1LW8_000733"
     CDS             3910..4002
                     /locus_tag="AC1LW8_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772598.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            4201..4968
                     /gene="rpsB"
                     /locus_tag="AC1LW8_000734"
     CDS             4201..4968
                     /gene="rpsB"
                     /locus_tag="AC1LW8_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIP4772599.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            5002..5115
                     /locus_tag="AC1LW8_000735"
     CDS             5002..5115
                     /locus_tag="AC1LW8_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772600.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5150..6031
                     /gene="tsf"
                     /locus_tag="AC1LW8_000736"
     CDS             5150..6031
                     /gene="tsf"
                     /locus_tag="AC1LW8_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIP4772601.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6168..6890
                     /gene="pyrH"
                     /locus_tag="AC1LW8_000737"
     CDS             6168..6890
                     /gene="pyrH"
                     /locus_tag="AC1LW8_000737"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIP4772602.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6909..7463
                     /gene="frr"
                     /locus_tag="AC1LW8_000738"
     CDS             6909..7463
                     /gene="frr"
                     /locus_tag="AC1LW8_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIP4772603.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7835..8605
                     /locus_tag="AC1LW8_000739"
     CDS             7835..8605
                     /locus_tag="AC1LW8_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIP4772604.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8612..9394
                     /locus_tag="AC1LW8_000740"
     CDS             8612..9394
                     /locus_tag="AC1LW8_000740"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIP4772605.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            9606..10892
                     /gene="rseP"
                     /locus_tag="AC1LW8_000741"
     CDS             9606..10892
                     /gene="rseP"
                     /locus_tag="AC1LW8_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIP4772606.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            10912..12615
                     /locus_tag="AC1LW8_000742"
     CDS             10912..12615
                     /locus_tag="AC1LW8_000742"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIP4772607.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKYSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            12879..17189
                     /locus_tag="AC1LW8_000743"
     CDS             12879..17189
                     /locus_tag="AC1LW8_000743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIP4772608.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINSIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            17479..17946
                     /gene="rimP"
                     /locus_tag="AC1LW8_000744"
     CDS             17479..17946
                     /gene="rimP"
                     /locus_tag="AC1LW8_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIP4772609.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            17967..19142
                     /gene="nusA"
                     /locus_tag="AC1LW8_000745"
     CDS             17967..19142
                     /gene="nusA"
                     /locus_tag="AC1LW8_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIP4772610.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTIVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            19163..19447
                     /gene="rnpM"
                     /locus_tag="AC1LW8_000746"
                     /gene_synonym="ylxR"
     CDS             19163..19447
                     /gene="rnpM"
                     /locus_tag="AC1LW8_000746"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIP4772611.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            19444..19761
                     /locus_tag="AC1LW8_000747"
     CDS             19444..19761
                     /locus_tag="AC1LW8_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HIP4772612.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            19766..21883
                     /gene="infB"
                     /locus_tag="AC1LW8_000748"
     CDS             19766..21883
                     /gene="infB"
                     /locus_tag="AC1LW8_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIP4772613.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKMAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            22269..22619
                     /gene="rbfA"
                     /locus_tag="AC1LW8_000749"
     CDS             22269..22619
                     /gene="rbfA"
                     /locus_tag="AC1LW8_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIP4772614.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            22789..23706
                     /gene="truB"
                     /locus_tag="AC1LW8_000750"
     CDS             22789..23706
                     /gene="truB"
                     /locus_tag="AC1LW8_000750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIP4772615.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            23721..24692
                     /gene="ribF"
                     /locus_tag="AC1LW8_000751"
     CDS             23721..24692
                     /gene="ribF"
                     /locus_tag="AC1LW8_000751"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIP4772616.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            24807..25076
                     /gene="rpsO"
                     /locus_tag="AC1LW8_000752"
     CDS             24807..25076
                     /gene="rpsO"
                     /locus_tag="AC1LW8_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIP4772617.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT         9216 a         3432 c         4953 g         7493 t
ORIGIN      
        1 ttagtaatta atttaaaaaa gaggtattct aattaacagg ttttccgatt agttacaatt
       61 atttaattct caaaagattt agaattgatt atcaaattac tgtaagccct ttgctgtata
      121 tgctacaatt cttattgatg gagggtaaat gtattgaatc atattcaaga agcgttttta
      181 aatacattga aagttgaacg gaatttttcg gaacatacat tgaaatcata tcaagatgac
      241 ttaattcagt ttaatcaatt tttagaacaa gaacatttac agttgaaaac ttttgaatac
      301 agagatgcta gaaattattt gagctattta tattcaaatc atttgaaaag aacatctgtt
      361 tctcgtaaaa tctcaacgtt aagaactttc tatgaatatt ggatgacgct tgatgagaac
      421 attattaatc catttgttca attagtacat ccgaaaaaag aaaaatatct tccgcaattc
      481 ttttacgaag aagaaatgga agcgttattt aaaactgtag aagaggacac ttcaaaaagt
      541 ttacgggatc gagttattct tgaattgtta tatgctacag gtatccgtgt ttcggaatta
      601 gttaatatta aaaaacaaga tatagatttt tacgcgaatg gtgttaccgt attaggaaaa
      661 gggagcaaag agcgctttgt accgtttggt gcttattgta gacaaagcat cgaaaattat
      721 ttagaacatt tcaaaccaat tcagtcatgc aatcatgatt ttcttattgt aaatatgaag
      781 ggtgaagcaa tcactgaacg cggtgtacga tatgttttaa atgatattgt taaacgaaca
      841 gcaggcgtaa gtgagattca tccccacaag ctcagacata catttgcaac gcatttattg
      901 aatcaaggtg cagacctaag aacagtacaa tcgttattag gtcatgttaa tttatcaaca
      961 actggtaaat atacacacgt atctaaccaa caattaagaa aagtgtatct aaatgcacat
     1021 cctcgagcga aaaaggagaa tgaaacatga gtaatacaac attacatgca acaacaattt
     1081 atgctgtaag acataatggg aaagcagcta tggctggaga tgggcaagta acgcttggtc
     1141 aacaagtcat catgaaacaa acggcaagaa aagtgcgacg tttatatgaa ggtaaagtgt
     1201 tagctggttt cgcaggtagt gtagcagatg cgtttacgtt atttgaaaaa ttcgaaacaa
     1261 aattacaaca gtttagtggt aacttagaaa gagctgctgt tgaattggca caagaatggc
     1321 gaggcgataa acaattacgt caattagaag ctatgctaat tgtaatggat aaagatgcta
     1381 ttttagttgt cagtggaact ggcgaagtta ttgccccaga tgatgacctt atcgctattg
     1441 gatcaggagg caactacgca ttaagcgcag gacgtgcatt gaaacgccat gcatcgcatt
     1501 tgtctgctga agaaatggca tatgagagct tgaaagtagc ggctgatatt tgtgtcttta
     1561 ccaacgataa tattgttgtt gaaacactat aataatcaga gcacgataaa taattacgag
     1621 caattaattt tagttaaaag acggaggaat gaaattaatg gatacagctg gaataagatt
     1681 aactccaaaa gaaatcgtat ctaaattaaa tgaatacatc gttggacaaa atgatgctaa
     1741 acgtaaagtg gcaattgcct tacgtaatcg atacagaaga agtttattag atgaggaatc
     1801 aaagcaagaa atttcaccta aaaatatttt gatgattgga ccaactggcg ttggtaaaac
     1861 tgaaattgca agaagaatgg ccaaagttgt cggcgcgcca tttataaaag tagaagctac
     1921 taaatttact gaggtaggtt atgtaggacg agatgttgaa agtatggtta gagatcttgt
     1981 tgatgtttca gtaagattag tcaaggcaca gaaaaaatca ttggtacaag atgaagcaac
     2041 agctaaggcc aatgaaaaac ttgttaagtt attagttcca agtatgaaaa agaaagcgtc
     2101 tcaaacgaat aatcctttag agtcactttt cggaggtgca attccaaatt tcggacaaaa
     2161 taacgaagat gaagaagaac cacctactga ggaaattaaa acaaaacgtt ctgaaattaa
     2221 gagacagcta gaagaaggca aacttgaaaa agaaaaggta agaattaaag tcgaacaaga
     2281 tcctggtgct ttaggtatgc taggtacaaa tcaaaatcag caaatgcaag agatgatgaa
     2341 tcaattaatg cctaaaaaga aagttgagcg agaagttgct gttgagacgg caaggaaaat
     2401 cttagctgat agttatgcgg atgaactaat tgatcaagaa agcgctaacc aagaagcgct
     2461 tgaattagca gaacaaatgg gtatcatctt tatagatgaa atcgacaaag ttgcgacgaa
     2521 taatcataat agtggtcaag atgtctcaag acaaggtgtt caaagagata ttttacctat
     2581 acttgaaggt agcgttattc aaaccaaata tggtactgtg aatactgaac atatgctgtt
     2641 tataggtgct ggagctttcc atgtatctaa gccgagtgac ttgataccag aattgcaagg
     2701 tcgttttccg attagagttg aacttgatag tttatcggta gaagattttg taagaatttt
     2761 gacagaacca aaattgtcat taattaaaca atatgaagca ttgcttcaaa cagaagaagt
     2821 tactgtaaac tttaccgatg aagcaattac tcgtttagct gagattgctt atcaagtaaa
     2881 tcaagataca gacaacattg gtgcacgtcg acttcataca attttagaaa agatgttaga
     2941 agatttatca ttcgaagcac caagtatgcc gaatgcagtt gtagatatta ccccacaata
     3001 tgttgatgat aaattaaaat caatttcaac aaataaagat ttaagtgcat ttattctata
     3061 aaaatataca aaaggagaaa aattcatgag cttattatct aaaacgagag agttaaacac
     3121 gttacttcaa aaacacaaag gtattgcggt tgattttaaa gatgtagcac aaacgattag
     3181 tagcgtaact gtaacaaatg tatttattgt atcgcgtcga ggtaaaattt tagggtcgag
     3241 tctaaatgaa ttattaaaaa gtcaaagaat tattcaaatg ttggaagaaa gacatatccc
     3301 aagtgaatat acagaacgat taatggaagt taaacaaaca gaatcaaata ttgatatcga
     3361 caatgtatta acagtttttc cacctgaaaa cagagaatta ttcatagata gtcgtacaac
     3421 tatcttccca attttaggtg gaggagaaag attaggtaca ttagtacttg gtcgagtaca
     3481 tgatgatttt aatgaaaatg atttggtact aggtgaatat gctgctacag ttattggtat
     3541 ggaaatctta cgtgagaagc atagtgaagt agaaaaagaa gcgcgcgata aagctgctat
     3601 tacaatggca attaattcat tatcttattc tgaaaaagaa gcgattgaac atatctttga
     3661 agaacttggc ggtacggaag gcctattaat cgcatcaaaa gttgcagata gagttggtat
     3721 tactagatct gtaattgtaa atgcactacg taaattagaa agtgctggtg taattgaatc
     3781 acgttcttta ggaatgaaag gtactttcat taaagttaaa aaagaaaaat tcttagatga
     3841 attagaaaaa agtaaataag tcgatgagtc tgggacataa ttcaatgtcc tagtcgtgac
     3901 gatgttatct tggcggtagt caacgaaatg actttgatga ttatgttgaa gtcatttctt
     3961 tatttttgtc taattttcaa tcaaattgat attccacatt aacatgatag aattaccttt
     4021 aaatttatga aaaaattctt gaaaaacatt tgctttagac acggcattat gttatattta
     4081 ttttcggcta taaagccaat acacacatat caaacgtgat tgtgtaaagg gtgcaatatt
     4141 tgtatattgt ctttacaata gtttgctatg gaggtaatta accaatagga ggaatttata
     4201 atggcagtaa tttcaatgaa acaattacta gaagcgggtg ttcacttcgg tcaccaaaca
     4261 cgtcgttgga acccaaaaat gaaaaaatat atcttcactg agagaaatgg tatttatatc
     4321 atcgacttac aaaaaacagt gaaaaaagta gacgaggcat acaacttctt gaaacaagtt
     4381 tcagaagatg gtggacaagt cttattcgta ggaactaaaa aacaagcaca agaatcagtt
     4441 aaatctgaag cagaacgtgc tggtcaattc tacattaacc aaagatggtt aggtggatta
     4501 ttaactaact ataaaacgat ctcaaaacga atcaaacgta tttctgaaat tgaaaaaatg
     4561 gaagaagatg gtttattcga agtattacct aaaaaagaag tagtagaact taaaaaagaa
     4621 tacgaccgtt taatcaaatt cttaggcgga attcgtgata tgaaatcaat gcctcaagca
     4681 ttattcgtag ttgacccacg taaagagcgt aatgcaattg ctgaagctcg taaattaaat
     4741 attcctatcg taggtatcgt tgacactaac tgtgatcctg acgaaattga ctacgttatc
     4801 ccagcaaacg acgatgctat ccgtgcggtt aaattattaa ctgctaaaat ggcagatgca
     4861 atcttagaag gtcaacaagg cgtttctaat gaagaagtag ctgcagaaca aaacatcgat
     4921 ttagatgaaa aagaaaaatc agaagaaaca gaagcaactg aagaataatc aactgttgaa
     4981 tctgacttag atatagttta aatgggtgat aagatattaa tgcttatcac cttttttaaa
     5041 aagaaaatcg aggcaaatta caaatattca attagagtat tagcaatctt gcctataata
     5101 atgctaaaat cataatatat aaaatgataa cttattggag gaataatgaa tggcaactat
     5161 ttcagcaaaa cttgttaaag aattacgtga aaaaactggc gcgggtatga tggattgtaa
     5221 aaaagcgcta actgaaactg atggtgacat cgataaagcg attgattacc tacgtgaaaa
     5281 aggtattgct aaagcagcta aaaaagcaga ccgtattgcg gctgaaggtt tagtacatgt
     5341 agaaactaaa ggtaacgacg cagttatcgt tgaaatcaac tctgaaacag actttgttgc
     5401 tcgtaacgaa ggattccaag agttagttaa agaaatcgct aatcaagtat tagatacaaa
     5461 agctgaaact gttgaagctt taatggaaac aactttacca aatggtaaat cagttgatga
     5521 aagaattaaa gaagcaattt caacaatcgg tgaaaaatta agtgttcgtc gttttgctat
     5581 cagaactaaa actgataacg atgctttcgg cgcttactta cacatgggtg gacgcattgg
     5641 tgtattaaca gttgttgaag gttcaactga cgaagaagca gcaagagacg ttgctatgca
     5701 tatcgctgca atcaacccta aatatgtttc ttctgaacaa gttagcgaag aagaaatcaa
     5761 ccacgaaaga gaagttttaa aacaacaagc attaaatgaa ggtaaaccag aaaacatcgt
     5821 tgaaaaaatg gtggaaggac gtttacgtaa atacttacaa gaaatttgtg ctgtagatca
     5881 agacttcgtt aaaaaccctg atgtaacagt tgaagctttc ttaaaaacaa aaggtggaaa
     5941 acttgttgac ttcgtacgct atgaagtagg cgaaggtatg gaaaaacgcg aagaaaactt
     6001 tgcggatgaa gttaaaggac aaatgaaata atctgtcata aagtaaaaca aggaagaaga
     6061 cacctttaat gttgctttat taaaatgtaa atcattctaa taaaacgaca actgtgtctt
     6121 ctttacttgt atatgttaca tatattcacg atagagagga taagaaaatg gctcaaattt
     6181 ctaaatataa acgtgtagtt ttgaaactaa gtggtgaagc gttagctgga gaaaaaggat
     6241 ttggcataaa tccagtaatt attaaaagtg ttgctgagca agtggctgaa gttgctaaaa
     6301 tggactgtga aatcgcagta atcgttggtg gcggaaacat ttggagaggt aaaacaggta
     6361 gtgacttagg tatggaccgt ggaactgctg attacatggg tatgcttgca actgtaatga
     6421 atgccttagc attacaagat agtttagaac aattggattg tgatacacga gtattaacat
     6481 ctattgaaat gaagcaagtg gctgaacctt atattcgtcg tcgtgcaatt agacacttag
     6541 aaaagaaacg cgtagttatt tttgctgcag gtattggaaa cccatacttc tctacagata
     6601 ctacagcggc attacgtgct gcagaagttg aagcagatgt tattttaatg ggcaaaaata
     6661 atgtagatgg tgtatattct gcagatccta aagtaaacaa agatgcggta aaatatgaac
     6721 atttaacgca tattcaaatg cttcaagaag gtttacaagt aatggattca acagcatcct
     6781 cattctgtat ggataataac attccgttaa ctgttttctc tattatggaa gaaggaaata
     6841 ttaaacgtgc tgttatgggt gaaaagatag gtacgttaat tacaaaataa atttagaggt
     6901 gtaaaataat gagtgacatt attaatgaaa ctaaatcaag aatgcaaaaa tcaatcgaaa
     6961 gcttatcacg tgaattagct aatatcagtg caggaagagc taattcaaat ttattaaacg
     7021 gcgtaacagt tgattactat ggtgcaccaa cacctgtaca acaattagca agcatcaatg
     7081 ttccagaagc acgtttactt gttatttctc catacgacaa aacttctgta gctgacatcg
     7141 aaaaagcgat aatagcggct aacttaggtg tcaacccaac aagtgatggt gaagtgatac
     7201 gtattgcagt acctgcctta acagaagagc gtagaaaaga gcgcgttaaa gatgttaaga
     7261 aaattggtga agaagctaaa gtatctgttc gaaatattcg tcgtgatatg aatgatcagt
     7321 tgaaaaaaga tgaaaaaaat ggcgacatta ctgaagatga gttgagaagt ggtactgaag
     7381 atgttcagaa agcaacagac aattcaataa aagaaattga tcaaatgatt gctgataaag
     7441 aaaaagatat tatgtcagta taaaactaat atacaatgac atattaaaat gccagtatta
     7501 aacgataatg taacatttaa aatgggcatg tttaattaaa tcaaagatgc atgtgataat
     7561 ttaaattcac aatgagcata aaaatggtgt ttaaacaagt taattaaaca tatactttat
     7621 aaataatagg cattaggtat attgctataa taaagttatg taatttttaa cctcagtatg
     7681 tatgtcacat ttctggtgta aactgtaccg agtcagactt tggtacagtt ttttatttgc
     7741 ttattcaatg cattaaatga gtatgataaa atgataatga ttgtttagta acttatacta
     7801 tatgacagag atgatcaggc tcggaggaaa gaccatgttt aaaaagctaa taaataaaaa
     7861 gaacactata aataattata atgaagaatt agactcgtct aatatacctg aacatatcgc
     7921 tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta gaattaaagg
     7981 tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg atattggtgt
     8041 taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg aaagtgaagt
     8101 aaattatatt atgaatttgc ctgtcaattt cttaaagaca ttcttaccgg aactaattga
     8161 aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat caacgataga
     8221 agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat taatatttgc
     8281 aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt ttgacgagct
     8341 tcatcaacaa ggtttaaata gtgatatcat agatgaaaca tatataaaca atcatttaat
     8401 gacaaaagac tatcctgatc cagagttgtt aattcgtact tcaggagaac aaagaataag
     8461 taatttcttg atttggcaag tttcgtatag tgaatttatc tttaatcaaa aattatggcc
     8521 tgactttgac gaagatgaat taattaaatg tataaaaatt tatcagtcac gtcaaagacg
     8581 ctttggcgga ttgagtgagg agtagtatag tatgaaagtt agaacgctga cagctattat
     8641 tgccttaatc gtattcttgc ctatcttgtt aaaaggcggc cttgtgttaa tgatatttgc
     8701 taatatatta gcattgattg cattaaaaga attgttgaat atgaatatga ttaaatttgt
     8761 ttcagttcct ggtttaatta gtgcagttgg tcttatcatc attatgttgc cacaacatgc
     8821 agggccatgg gtacaagtaa ttcaattaaa aagtttaatt gcaatgagct ttattgtatt
     8881 aagttatact gtcttatcta aaaacagatt tagttttatg gatgctgcat tttgcttaat
     8941 gtctgtggct tatgtaggca ttggttttat gttcttttat gaaacgagat cagaaggatt
     9001 acattacata ttatatgcct ttttaattgt ttggcttaca gatacagggg cttacttgtt
     9061 tggtaaaatg atgggtaaac ataagctttg gccagtaata agtccgaata aaacaatcga
     9121 aggattcata ggtggcttgt tctgtagttt gatagtacca cttgcaatgt tatattttgt
     9181 agatttcaat atgaatgtat ggatattact tggagtgaca ttgattttaa gtttatttgg
     9241 tcaattaggt gatttagtgg aatcaggatt taagcgtcat ttcggcgtta aagactcagg
     9301 tcgtatacta cctggacacg gtggtatttt agaccgattt gacagcttta tgtttgtgtt
     9361 accattatta aatattttat taatacaatc ttaatgctga gaacaaatca ataaacgtaa
     9421 agaggagttg ctgagataat ttaatgaatc tcagaactcc ttttgaaaat tatacgcaat
     9481 attaactttg aaaattagac gttatatttt gtgatttgtc agtatcatat tataatgact
     9541 tatgttacgt atacagcaat catttttaaa ataaaagaaa tttataaaca atcgaggtgt
     9601 agcgagtgag ctatttagtt acaataattg catttattat tgtttttggt gtactagtaa
     9661 ctgttcatga atatggccat atgttttttg cgaaaagagc aggcattatg tgtccagaat
     9721 ttgcgatcgg tatgggaccg aaaattttta gttttagaaa aaacgaaaca ctttacacta
     9781 ttaggttatt gcctgttggt ggatatgttc gtatggcagg agatggctta gaagagccac
     9841 cagtcgagcc cggtatgaac gttaaaatta aactgaatga agaaaatgaa ataacacata
     9901 tcatattaga tgatcatcat aagtttcaac aaattgaagc aatcgaagtt aaaaaatgtg
     9961 attttaagga tgacttattc atagaaggta tcactgctta tgataatgaa agacatcatt
    10021 ttaaaattgc tagaaagtct ttctttgttg aaaatggtag cttagttcaa attgctccga
    10081 gagacagaca atttgcacat aaaaagccat ggccgaaatt tttaacatta tttgcgggac
    10141 cgttatttaa ctttatatta gctttagtcc tatttattgg tcttgcatat tatcaaggca
    10201 cgcctacgtc tactgtagaa caagtcgcag ataagtatcc agctcaacaa gcaggattac
    10261 aaaaaggtga taagatcgtc caaattggca aatataaaat atctgaattt gatgatgttg
    10321 ataaggcgtt agataaagtt aaagataata agacgactgt taaatttgaa cgtgatggta
    10381 aaacaaagtc agttgaatta acacctaaaa agactgaaag aaaactgact aaagtaagtt
    10441 cagagacgaa gtatgttctc ggattccaac cagcgagtga acgtacgctc ttcaaaccaa
    10501 ttgtatatgg atttgaaagc tttttgaaag gtagtacact aatttttaca gctgtagtcg
    10561 gtatgttggc tagtatattt acgggcggat tctcatttga tatgttaaat ggtccggttg
    10621 gtatttatca taacgtcgac tcagttgtta aagcgggtat cattagctta attggttaca
    10681 ctgcgttatt aagtgtaaac ttaggtatta tgaatttaat tcctattcct gcactagacg
    10741 gtggtcgtat tttatttgtt atatatgaag cgattttcag aaaaccagtt aataaaaaag
    10801 cggaaacaac gattattgct attggtgcca ttttcatggt cgttattatg atattagtaa
    10861 cgtggaatga tattcgacga tatttcttat aatttaggag gataaataat tatgaagcaa
    10921 tccaaagttt ttataccaac gatgcgtgat gtgccatcag aagcagaagc acaaagtcat
    10981 cgtttattat tgaaatcggg tttgataaaa caaagtacaa gtgggattta tagttattta
    11041 ccgctagcaa cacgtgtgtt aaataatatt actgcaattg tgcgacaaga aatggaacgt
    11101 atcgattctg ttgaaatttt aatgccagcg ttacaacaag ctgaattatg ggaagaatca
    11161 ggacgttggg gtgcatatgg cccagaatta atgcgtttac aagatagaca tggaagacaa
    11221 tttgcattag gtccaacaca tgaagaatta gttacatcaa tagtaagaaa tgaattgaaa
    11281 tcatacaaac aattaccgat gacattattc caaattcaat ctaaattccg tgatgaaaag
    11341 agaccacgtt ttggtttact tcgtgggcgt gaatttatta tgaaagatgc gtattcattc
    11401 catgctgacg aggcatcatt agatcaaacg tatcaagata tgtatcaagc gtatagccgt
    11461 atttttgaga gagttggcat taacgcaaga ccagtagttg cagattcagg tgctataggc
    11521 ggtagccata cacatgaatt tatggcatta agtgctatcg gtgaggatac aatcgtttac
    11581 agtaaagaaa gtgactatgc tgctaatatc gaaaaagcag aagtcgttta cgaaccaaat
    11641 cataagtatt ctactgtgca acctttagaa aaaattgaaa caccaaatgt taagactgca
    11701 caagaattgg cagacttctt aggtagacca gtagatgaaa tcgttaaaac gatgattttc
    11761 aaagttgatg gcgaatatat tatggtttta gtgcgtggcc atcatgaaat taatgacatt
    11821 aaattaaaat cttatttcgg cacagataat attgaattag caacacaaga cgaaattgtt
    11881 aatttagttg gtgcaaatcc gggttcacta ggtcctgtta ttgataaaga aatcaaaatt
    11941 tatgcagata attttgtgca agatttaaat aatttagttg tcggtgctaa cgaagatggc
    12001 tatcacttaa ttaatgtaaa tgtaggtaga gacttcaacg ttgatgaata tggcgatttc
    12061 cgttttattt tagaaggcga aaagttaagt gatggttcag gcgttgcaca ttttgctgaa
    12121 ggtattgaag ttggtcaagt attcaaattg ggtactaagt attcagaatc aatgaatgct
    12181 acattcttag ataaccaagg aaaagcccaa cctttaatta tgggttgtta cggtattgga
    12241 atttctagaa cgctgagtgc gattgttgaa caaaatcacg atgataatgg aattgtttgg
    12301 cctaaatcag ttactccatt tgatttacat ttaatttcta ttaatcctaa gaaagatgat
    12361 caacgagaac tagcggatgc actatatgct gaatttaata ctaaatttga tgtgttgtac
    12421 gatgatcgtc aggaacgtgc gggtgtcaaa tttaatgatg ccgatttaat tggtttacca
    12481 ctgcgaattg ttgttggtaa acgtgcatcg gaaggtattg tagaagttaa agaacgttta
    12541 acaggtgata gcgaagaagt tcacattgat gacttaatga ctgtcattac aaataaatat
    12601 gataacttaa aataattaag atcgaatgaa ttataagagt aggaaaaagc tgaaagaaat
    12661 cagatgctta tgtcctgctc ttattatttt tgatataatg attattcgat gaaaaatgac
    12721 tgaagacata gtataattaa agataaattt gttttaacaa tattatgatt agccaaatat
    12781 aaagcattta attttctatc attactatgc ctacataatc taaatattgt tcgaacacgt
    12841 aaaagtaatt tctatttaag gtggtaattg tcttggcaat gacagagcaa caaaaattta
    12901 aagtgcttgc tgatcaaatt aaaatttcaa atcaattaga tgctgaaatt ttaaattcag
    12961 gtgaactgac acgtatagat gtttctaaca aaaacagaac atgggaattt catattacat
    13021 taccacaatt cttagctcat gaagattatt tattatttat aaattcaata gagcaagagt
    13081 ttaaagatat cgccaacgtt acatgtcgtt ttacggtaac aaatggcacg aatcaagatg
    13141 aacatgcaat taaatacttt ggacactgta ttgaccaaac agctttatct ccaaaagtta
    13201 aaggtcaatt gaaacagaaa aagcttatta tgtctggaaa agtattaaaa gtaatggtat
    13261 caaatgacat tgaacgtaat cattttgata aggcatgcaa tggaagtctt atcaaagcgt
    13321 ttagaaattg tggttttgat atcgataaaa tcatcttcga aacaaatgat aatgatcaag
    13381 aacaaaactt agcttcttta gaagcacata ttcaagaaga agacgaacaa agtgcacgat
    13441 tggcaacaga gaaacttgaa aaaatgaaag ctgaaaaagc gaaacaacaa gataacaacg
    13501 aaagtgctgt tgataagtgt caaattggta agccgattca aattgaaaat attaaaccaa
    13561 ttgaatctat tattgaggaa gagtttaaag ttgcaataga gggtgtcatt tttgatataa
    13621 acttaaaaga acttaaaagt ggtcgccata tcgtagaaat taaagtgact gactatacgg
    13681 actctttagt tttaaaaatg tttactcgta aaaacaaaga tgatttagaa cattttaaag
    13741 cgctaagtgt tggtaaatgg gttagggctc aaggtcgtat tgaagaagat acatttatta
    13801 gagatttagt tatgatgatg tctgatattg aagagattaa aaaagcgaca aaaaaagata
    13861 aggctgaaga aaagcgtgta gaattccact tgcatactgc aatgagccaa atggatggta
    13921 tacccaatat tggtgcgtat gttaaacagg cagcagactg gggacatcca gccattgcgg
    13981 ttacagacca taatgttgtg caagcatttc cagatgctca cgcagcagcg gaaaaacatg
    14041 gcattaaaat gatatacggt atggaaggta tgttagttga tgatggtgtt ccgattgcat
    14101 acaaaccaca agatgtcgta ttaaaagatg ctacttatgt tgtgttcgac gttgagacaa
    14161 ctggtttatc aaatcagtat gataaaatca tcgagcttgc agctgtgaaa gttcataacg
    14221 gtgaaatcat cgataagttt gaaaggttta gtaatccgca tgaacgatta tcggaaacga
    14281 ttatcaattt gacgcatatt actgatgata tgttagtaga tgcccctgag attgaagaag
    14341 tacttacaga gtttaaagaa tgggttggcg atgcgatatt cgtagcgcat aatgcttcgt
    14401 ttgatatggg ctttatcgat acgggatatg aacgtcttgg gtttggacca tcaacgaatg
    14461 gtgttatcga tactttagaa ttatctcgta cgattaatac tgaatatggt aaacatggtt
    14521 tgaatttctt agctaaaaaa tatggcgtag aattaacgca acatcaccgt gccatttatg
    14581 atacagaagc aacagcttac attttcataa aaatggttca acaaatgaaa gaattaggcg
    14641 tattaaatca taacgaaatc aacaaaaaac tcagtaatga agatgcatat aaacgtgcaa
    14701 gacctagtca tgtcacatta attgtacaaa accaacaagg tcttaaaaat ctatttaaaa
    14761 ttgtaagtgc atcattggtg aagtatttct accgtacacc tcgaattcca cgttcattgt
    14821 tagatgaata tcgtgaggga ttattggtag gtacagcgtg tgatgaaggt gaattattta
    14881 cggcagttat gcagaaggac cagagccaag ttgaaaaaat tgccaaatat tatgatttta
    14941 ttgaaattca accaccggca ctttatcaag atttaattga tagagagctt attagagata
    15001 ctgaaacatt acatgaaatt tatcaacgtt taatacatgc aggtgacaca gcgggtatac
    15061 ctgttattgc gacaggaaat gcacactatt tgtttgaaca tgatggtatc gcacgtaaaa
    15121 ttttaatagc atcacaaccc ggcaatccac ttaatcgctc aactttaccg gaagcacatt
    15181 ttagaactac agatgaaatg ttaaacgagt ttcatttttt aggtgaagaa aaagcgcatg
    15241 aaattgttgt gaaaaataca aacgaattag cagatcgaat tgaacgtgtt gttcctatta
    15301 aagatgaatt atacacaccg cgtatggaag gtgctaacga agaaattaga gaactaagtt
    15361 atgcaaatgc gcgtaaactg tatggtgaag acctgcctca aatcgtaatt gatcgattag
    15421 aaaaagaatt aaaaagtatt atcggtaatg gatttgcggt aatttactta atttcgcaac
    15481 gtttagttaa aaaatcatta gatgatggat acttagttgg ttcccgtggt tcagtaggtt
    15541 ctagttttgt agcgacaatg actgagatta ctgaagtaaa cccgttaccg ccacactata
    15601 tttgtccgaa ctgtaaaacg agtgaatttt tcaatgatgg ttcagtagga tcaggatttg
    15661 atttacctga taagacgtgt gaaacttgtg gagcgccact tattaaagaa ggacaagata
    15721 ttccgtttga aacattttta ggatttaagg gagataaagt tcctgatatc gacttaaact
    15781 ttagtggtga atatcaaccg aatgcccata actacacaaa agtattattt ggtgaggata
    15841 aagtattccg tgcaggtaca attggtactg ttgctgaaaa gactgctttt ggttatgtta
    15901 aaggttattt gaatgatcaa ggcatccaca aaagaggtgc tgaaatagat cgactcgtta
    15961 aaggatgtac aggtgttaaa cgtacaactg gacagcatcc agggggtatt attgtagtac
    16021 ctgattacat ggatatttat gattttacgc cgatacaata tcctgccgat gatcaaaatt
    16081 cagcatggat gacgacacat tttgatttcc attctattca tgataatgta ttaaaacttg
    16141 atatacttgg acacgatgat ccaacaatga ttcgtatgct tcaagattta tcgggcattg
    16201 atccaaaaac gatacctgta gatgacaaag aagtcatgca aatatttagt acacctgaaa
    16261 gtttaggtgt tactgaagat gaaattttat gtaaaacagg tacgtttggg gttccagaat
    16321 tcggtacagg gttcgtgcgt caaatgttag aagatacaaa gccaacaaca ttttctgaat
    16381 tagttcaaat ctcaggatta tctcatggta cagatgtgtg gttaggcaat gctcaagaat
    16441 taattaaaac cggtatatgt gatttatcaa gtgtaattgg ttgtcgtgat gatatcatgg
    16501 tttatttaat gtatgctggt ttagaaccat caatggcttt taaaataatg gagtcagtac
    16561 gtaaaggtaa aggtttaact gaagaaatga ttgaaacgat gaaagaaaat gaagtgccgg
    16621 attggtattt agattcatgt cttaaaatta agtacatgtt ccctaaagcc catgcagcag
    16681 catacgtttt aatggcagta cgtatcgcat atttcaaagt acatcatcca ctttattact
    16741 atgcatctta ctttacaatt cgtgcgtcag actttgattt aatcacgatg attaaagata
    16801 aaacaagcat tcgaaatact gtaaaagaca tgtattctcg ctatatggat ctaggtaaaa
    16861 aagaaaaaga cgtattaaca gtcttggaaa ttatgaatga aatggcgcat cgaggttatc
    16921 gcatgcaacc gattagttta gaaaagagtc aggcgttcga atttatcatt gaaggcgata
    16981 cacttattcc gccgttcata tcagtgcctg ggcttggcga aaacgttgcg aaacgaattg
    17041 ttgaagctcg tgacgatggc ccatttttat caaaagaaga tttaaacaaa aaagctggat
    17101 tatctcagaa aattattgag tatttagatg agttaggctc attaccgaat ttaccagata
    17161 aagctcaact ttcgatattt gatatgtaaa atgaaataat caaggtattt atttaatgcg
    17221 tatggcgtag ttaaagaaat acaaaattgt tgctggacat aaaattatgc ctgtatttct
    17281 tttcaacgtc ttacgagtct attcgaatgt aatggtgaaa taaatgaaca aacttttaca
    17341 agaatctctg attaatagtg aagtcatttg tttcaagcat aaacttatgc tataattaag
    17401 ttgcttaaaa attagtgaac tcaggcagaa gagtgggaga ttcccgctct tttctatttg
    17461 ccaaaaaggg aggcctgtat gagtaaaatt acagaacaag tagaagtgat tgttaaacca
    17521 attatggaag acttgaattt tgaacttgta gacgttgaat atgtcaaaga gggtagagat
    17581 cattttctta gaatctctat tgataaagaa ggtggcgtag atttaaatga ttgtacgcta
    17641 gcttctgaaa aaataagtga agctatggat gcaaatgatc ctattcctga aatgtattat
    17701 ttagacgtag cgtcacctgg tgcagaacgt ccaattaaaa aagaacaaga tttccaaaat
    17761 gcaataacta aacctgtatt tgtttcttta tatgtaccaa ttgaaggtga aaaggaatgg
    17821 ttaggcattt tacaagaagt caataatgaa acaattgtag tacaagttaa aatcaaagca
    17881 agaacgaaag atatagagat accgagagac aaaatagcaa aagcacgtca cgcagttatg
    17941 atttaacgtg atgaggagga aaaaacgtgt caagtaatga attattatta gctactgagt
    18001 atttagaaaa agaaaagaag attcctagag cagtattaat tgatgctatt gaagcagctt
    18061 taattactgc atacaaaaag aattatgata gtgcaagaaa tgtccgtgtg gaattaaata
    18121 tggatcaagg tactttcaaa gttatcgctc gtaaagatgt tgttgaagaa gtatttgacg
    18181 acagagatga agtggattta agtacagcgc ttgttaaaaa ccctgcatat gaaattggtg
    18241 atatatacga agaagatgta acacctaaag attttggtcg tgtaggtgct caagcagcga
    18301 aacaagcagt aatgcaacgt cttcgtgatg ctgaacgtga aattttattt gaagaattta
    18361 tagacaaaga agaagacata cttactggaa ttattgaccg tgttgaccat cgttatgtat
    18421 atgtgaactt aggtcgtatc gaagctgttt tatctgaagc agaaagaagt cctaacgaaa
    18481 aatatattcc taacgaacgt atcaaagtat atgttaacaa agtggaacaa acgacaaaag
    18541 gtcctcaaat ctatgtttct cgtagccatc caggtttatt aaaacgttta tttgaacaag
    18601 aagttccaga aatttacgat ggtactgtaa ttgttaaatc agtagcacgt gaagctggcg
    18661 atcgctctaa aattagtgtc ttctctgaaa acaatgatat agatgctgtt ggtgcatgtg
    18721 ttggtgccaa aggcgcacgt gttgaagctg ttgttgaaga gctaggtggc gaaaaaatcg
    18781 acatcgttca atggaatgaa gatccaaaag tatttgtaaa aaatgcttta agcccttctc
    18841 aagttttaga agttattgtt gatgaaacaa atcaatctac aatagttgtt gttcctgatt
    18901 atcaattgtc attagcgatt ggtaaaagag gacaaaacgc acgtctagct gctaaattaa
    18961 ccggctggaa aattgatatt aaatcagaaa cagatgcgcg cgaagcgggt atctatccag
    19021 tagttgaagc tgaaaaagta actgaagaag atgttgcttt agaagatgct gacacaacag
    19081 aatcaaccga agaggtaaat gatgtttcag ttgaaacaaa tgtagagaaa gaatctgaat
    19141 aataggttgg agtgaagtat ctatgaaaaa gaaaaaaatt ccgatgcgaa aatgtattct
    19201 ttcaaatgaa atgcatccca aaaaagatat gattcgtgtt gttgttaata aagaaggcga
    19261 aatctttgcg gatgttactg gaaagaaaca aggccgtggc gcatatgttt ctaaagatgt
    19321 tgctatggtt gaaaaagcac aacaaaaaga aattttagaa aaatatttta aagcatctaa
    19381 agagcaattg gatcctgttt ataaagaaat tattagatta atttatagag aagagatccc
    19441 aaaatgagta tagatcaaat attaaacttt ttaggattag caatgagagc tggtaaagta
    19501 aaaacaggtg aatcagtcat tgttaatgag ataaaaaaag gaaatttaaa gctcgttatt
    19561 gttgcaaatg atgcgtctga taatacagct aaattaatta cagataaatg taagagttac
    19621 aaagttccat tcagaaagtt tggaaatcga aatgaattgg gaatagcact tggaaaaggt
    19681 gagcgtgtta atgtagggat tactgaccca ggctttgcta aaaagttgct atcaatgata
    19741 gatgaatatc ataaggagtg attatatgag taaacaaaga atttacgaat atgcgaaaga
    19801 attaaatcta aagagtaaag agattataga tgagttaaaa agcatgaata ttgaggtttc
    19861 aaatcatatg caagctttgg aagatgacca aattaaagca ttagataaaa agttcaaaaa
    19921 agaacaaaag aacgacaata aacaaagcac tcaaaataat caccaaaaat caaacaatca
    19981 aaaccaaaat aaagggcaac aaaaagataa caaaaagaat caacaacaaa ataataaagg
    20041 caacaaaggc aataaaaaga ataatagaaa taataagaaa aataacaaga ataataaacc
    20101 acaaaatcaa ccagctgctc caaaagaaat accatcaaaa gtgacatatc aagaaggtat
    20161 tacagtaggc gaatttgcgg ataaattaaa tgttgaatca tcagaaatta tcaaaaaatt
    20221 attcttactt ggtattgttg ctaatatcaa tcaatcatta aatcaagaaa caatcgaatt
    20281 aattgccgat gattatggcg ttgaggttga agaggaagtt gtgattaatg aagaagattt
    20341 atcaatctac ttcgaagacg aaaaagatga tccagaagca attgagagac cagcagttgt
    20401 aacaattatg ggacatgttg accatggtaa aactacttta ttagattcaa ttcgtcatac
    20461 aaaagttaca gcaggtgaag caggtggaat cactcaacat attggtgcat atcaaattga
    20521 aaacgatggc aaaaaaatca ctttcttaga tacaccggga catgctgcat ttacaacgat
    20581 gcgtgcgcgt ggtgcacaag taacagatat tacaatttta gtagtagcag ctgacgatgg
    20641 tgttatgcca caaacaattg aagcaattaa ccatgctaaa gaagcagaag taccaattat
    20701 tgttgcagta aataaaattg ataaaccaac ttcaaatcct gatcgagtta tgcaagaatt
    20761 aactgaatat ggtttaattc ctgaagattg gggtggcgaa acaattttcg ttccactttc
    20821 tgcattaagt ggtgatggta tcgacgattt attagaaatg ataggattag ttgcagaagt
    20881 tcaagaactt aaagcaaatc ctaaaaaccg tgctgttggt acagttatcg aagctgaatt
    20941 agataaatca cgtggtcctt ctgcatcatt attagtacaa aacggtacat taaatgttgg
    21001 ggatgcgatt gtagttggta atacttacgg ccgtattcgt gcaatggtta atgacttagg
    21061 tcaaagaatc aaaatggctg gtccatcaac gcctgttgaa attacaggta ttaatgatgt
    21121 gccacaagct ggggatcgct ttgttgtatt tagtgatgaa aaacaagctc gtcgtattgg
    21181 tgaatcaaga cacgaagcta gcattgttca acaacgtcaa gaaagtaaaa atgtttcatt
    21241 agataacctg tttgaacaaa tgaaacaagg tgaaatgaaa gatttaaacg ttattattaa
    21301 aggtgatgtt caaggttctg ttgaagcttt agctgcatca ttaatgaaaa ttgatgttga
    21361 aggcgtaaat gttcgtatca ttcatacagc ggttggtgca attaatgagt cagacgtgac
    21421 acttgctaat gcctcaaatg gtattatcat tggtttcaat gttcgtccag acagtggtgc
    21481 aaaacgtgct gcagaagctg aaaatgttga tatgcgttta cacagagtta tttataatgt
    21541 tatcgaagaa attgaatcag cgatgaaagg tttacttgat ccagaatttg aagaacaagt
    21601 tatcggacaa gctgaagttc gtcaaacatt caaagtttct aaagttggta ctattgctgg
    21661 atgttatgtt actgaaggta aaattacgcg aaatgctggt gtacgtatta ttcgtgatgg
    21721 tattgttcaa tatgaaggcg aattagatac acttaaacgt ttcaaagatg atgctaagga
    21781 agttgcaaaa ggttatgaat gtggtattac aattgaaaac tacaatgacc ttaaagaagg
    21841 cgatgttatc gaagcatttg aaatggttga aattaagcgt taattaaata aattacaagc
    21901 taaaagtata gttaagattg atatgctccc tataaatatt gcacttttta agtgtctact
    21961 ttatagggag catatttgat actagctttt ggttttttat tagaatagat tacctattaa
    22021 aagttacgtt atatggacat gattttgtat aaaattttgt ggtggcctag aatgattttt
    22081 aatgacaaaa tataatgtcg actattattg gaaaattttc tgttgaaatg cctatcttac
    22141 ggcaaacttt atttgatttt ataggcttaa tttattaaaa taacgtgtga gctaaaataa
    22201 ttgtttaagc attgttacac taaaaaatgc aaataacaat tgaacttaaa gataaagagg
    22261 tgacaagaat gagcagtatg agagcagagc gtgttggtga acaaatgaag aaggaattaa
    22321 tggatatcat caacaataaa gtcaaagatc ctcgagttgg ttttattaca attacagatg
    22381 ttgttttaac aaatgattta tcgcaggcta aagtattttt aactgtatta ggtaacgata
    22441 aagaagtaga aaatacattt aaagcacttg ataaagcaaa aggcttcatt aagtctgaat
    22501 taggttctag aatgcgatta cgtattatgc cggaattaat gtatgaatat gatcaatcaa
    22561 tcgaatatgg taataaaatt gaacgaatga ttcaagattt acacaaacaa gatagataat
    22621 ttagtgttag gtatctggaa aatgtttgat aatttcttaa tatcggtata ttaacattaa
    22681 acagttaata catagatgtg tagaaatagt taacattttc cagttttttt atgaataaat
    22741 tcagttgata cgctattaaa atatatttta aaaaaagaag gtgactatat gtataatggg
    22801 atattaccag tatataaaga gcgcggttta acaagtcatg acgttgtatt caaattgcgt
    22861 aaaatattaa aaactaaaaa aataggtcac acgggtacgc ttgatcccga agttgcaggc
    22921 gtgttaccgg tatgtatagg taatgcaacg agagttagtg attatgttat ggatatgggc
    22981 aaagcttatg aagcaactgt atcgatagga agaagtacaa cgactgaaga tcaaacgggt
    23041 gatacattgg aaacaaaagg tgtacactca gcagatttta ataaggacga tattgaccga
    23101 ttgttagaaa gttttaaagg tatcattgaa caaattccgc cgatgtactc atccgtcaaa
    23161 gtaaatggta aaaaattata tgaatatgcg cgtaataatg aaacagttga aagaccaaag
    23221 cgtaaagtta atattaaaga cattgggcgt atatctgaat tagattttaa agaaaatgag
    23281 tgtcatttta aaatacgcgt catctgtggt aaaggtacat atattagaac gctagcaact
    23341 gatattggtg tgaaattagg ctttccggca catatgtcga aattaacacg aatcgagtct
    23401 ggtggatttg tgttgaaaga tagccttaca ttagaacaaa taaaagaact tcatgagcag
    23461 gattcattgc aaaataaatt gtttccttta gaatatggat taaagggttt gccaagcatt
    23521 aaaattaaag attcgcacat aaaaaaacgt attttaaatg ggcagaaatt taataaaaat
    23581 gaatttgata acaaaattaa agaccaaatt gtatttattg atgatgattc agaaaaagta
    23641 ttagcaattt atatggtaca ccctacaaaa gaatcagaaa ttaaacctaa aaaagtcttt
    23701 aattaaagga gatagaattt atgaaagtca tagaagtgac acatcctata caatcaaaac
    23761 agtatattac agaggatgtt gcaatggcat tcggattttt cgatggcatg cataaaggtc
    23821 atgacaaagt ctttgatata ttaaacgaaa tagctgaggc acgcagttta aaaaaagcgg
    23881 tgatgacatt tgatccgcat ccgtctgtcg tgttgaatcc taaaagaaaa cgaacaacgt
    23941 atttaacgcc actttcagat aaaatcgaaa aaattagcca acatgatatt gattattgta
    24001 tagtggttaa tttttcatct aggtttgcta atgtgagcgt agaagatttt gttgaaaatt
    24061 atataattaa aaataatgta aaagaagtca ttgctggttt tgattttact tttggtaaat
    24121 ttggaaaagg taatatgact gtacttcaag aatatgatgc gtttaatacg acaattgtga
    24181 gtaaacaaga aattgaaaat gaaaaaattt ctacaacttc tattcgtcaa gatttaatca
    24241 atggtgagtt gcaaaaagcg aatgatgctt taggctatat atattctatt aaaggcactg
    24301 tagtgcaagg tgaaaaaagg ggaagaacta ttggcttccc aacagctaac attcaaccta
    24361 gtgatgatta tttgttacct cgtaaaggtg tttatgctgt tagtattgaa atcggcactg
    24421 aaaataaatt atatcgaggg gtagctaaca taggtgtaaa gccaacattt catgatccta
    24481 acaaagcaga agttgtcatc gaagtgaata tctttgactt tgaggataat atttatggtg
    24541 aacgagtgac cgtgaattgg catcatttct tacgtcctga gattaaattt gatggtatcg
    24601 acccattagt taaacaaatg aacgatgata aatcgcgtgc taaatattta ttagcagttg
    24661 attttggtga tgaagtagct tataatatct agagttgcgt atagttatat aaacaatcta
    24721 taccacacct ttttcttagt aggtcgaatc tccaacgcct aactcggatt aaggagtatt
    24781 caaacatttt aaggaggaaa ttgattatgg caatttcaca agaacgtaaa aacgaaatca
    24841 ttaaagaata ccgtgtacac gaaactgata ctggttcacc agaagtacaa atcgctgtac
    24901 ttactgcaga aatcaacgca gtaaacgaac acttacgtac acacaaaaaa gaccaccatt
    24961 cacgtcgtgg attattaaaa atggtaggtc gtcgtagaca tttattaaac tacttacgta
    25021 gtaaagatat tcaacgttac cgtgaattaa ttaaatcact tggtatccgt cgttaatctt
    25081 aatataacgt cttt
//
LOCUS       DBNENW010000023        24554 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNENW010000023 DBNENW010000000
VERSION     DBNENW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.023"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(290..394)
                     /locus_tag="AC1LW8_000753"
     CDS             complement(290..394)
                     /locus_tag="AC1LW8_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772618.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(555..653)
                     /locus_tag="AC1LW8_000754"
                     /pseudo
     CDS             complement(555..653)
                     /locus_tag="AC1LW8_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1074..1232
                     /locus_tag="AC1LW8_000755"
     CDS             1074..1232
                     /locus_tag="AC1LW8_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HIP4772619.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(1891..2748)
                     /locus_tag="AC1LW8_000756"
     CDS             complement(1891..2748)
                     /locus_tag="AC1LW8_000756"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4772620.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASPYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(2816..3598)
                     /locus_tag="AC1LW8_000757"
     CDS             complement(2816..3598)
                     /locus_tag="AC1LW8_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772621.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDEEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPMLAMIYVTHFIEEITSNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3829..5634)
                     /gene="glmS"
                     /locus_tag="AC1LW8_000758"
     CDS             complement(3829..5634)
                     /gene="glmS"
                     /locus_tag="AC1LW8_000758"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIP4772622.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6315..7853
                     /locus_tag="AC1LW8_000759"
     CDS             6315..7853
                     /locus_tag="AC1LW8_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HIP4772623.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            7888..10020
                     /locus_tag="AC1LW8_000760"
     CDS             7888..10020
                     /locus_tag="AC1LW8_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIP4772624.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKEFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDAINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMVLITVLEEPLILPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10032..10466
                     /locus_tag="AC1LW8_000761"
     CDS             10032..10466
                     /locus_tag="AC1LW8_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP4772625.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            10466..11572
                     /locus_tag="AC1LW8_000762"
     CDS             10466..11572
                     /locus_tag="AC1LW8_000762"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HIP4772626.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(11892..19397)
                     /gene="fmtB"
                     /locus_tag="AC1LW8_000763"
                     /pseudo
     CDS             complement(11892..19397)
                     /gene="fmtB"
                     /locus_tag="AC1LW8_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored DUF1542 repeat protein FmtB"
     gene            complement(19683..21038)
                     /gene="glmM"
                     /locus_tag="AC1LW8_000764"
     CDS             complement(19683..21038)
                     /gene="glmM"
                     /locus_tag="AC1LW8_000764"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIP4772627.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21065..21997)
                     /locus_tag="AC1LW8_000765"
     CDS             complement(21065..21997)
                     /locus_tag="AC1LW8_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIP4772628.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(21999..22808)
                     /gene="cdaA"
                     /locus_tag="AC1LW8_000766"
     CDS             complement(21999..22808)
                     /gene="cdaA"
                     /locus_tag="AC1LW8_000766"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIP4772629.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(22997..23905)
                     /gene="rocF"
                     /locus_tag="AC1LW8_000767"
     CDS             complement(22997..23905)
                     /gene="rocF"
                     /locus_tag="AC1LW8_000767"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HIP4772630.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPVVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(24043..24115)
                     /locus_tag="AC1LW8_000768"
     tRNA            complement(24043..24115)
                     /locus_tag="AC1LW8_000768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24080..24082),aa:Lys,seq:ttt)
     gene            complement(24119..24193)
                     /locus_tag="AC1LW8_000769"
     tRNA            complement(24119..24193)
                     /locus_tag="AC1LW8_000769"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24159..24161),aa:Gln,seq:ttg)
     gene            complement(24203..24286)
                     /locus_tag="AC1LW8_000770"
     tRNA            complement(24203..24286)
                     /locus_tag="AC1LW8_000770"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24250..24252),aa:Tyr,seq:gta)
     gene            complement(24316..24391)
                     /locus_tag="AC1LW8_000771"
     tRNA            complement(24316..24391)
                     /locus_tag="AC1LW8_000771"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24356..24358),aa:Val,seq:tac)
     gene            complement(24399..24470)
                     /locus_tag="AC1LW8_000772"
     tRNA            complement(24399..24470)
                     /locus_tag="AC1LW8_000772"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24435..24437),aa:Glu,seq:ttc)
     gene            complement(24472..24546)
                     /locus_tag="AC1LW8_000773"
     tRNA            complement(24472..24546)
                     /locus_tag="AC1LW8_000773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24512..24514),aa:Asn,seq:gtt)
BASE COUNT         7188 a         4418 c         3920 g         9028 t
ORIGIN      
        1 tactttttat ataattcgtg attacattct atacttttaa ttgtattttc tgctgtatgg
       61 aaagttggtt ttattaattc tgattatgtt cagaactaca attcataaga aaaatgtgtt
      121 aaaatatatt tgtagtaagt agaagcaaaa gatgaaaatc tttaactctt gaaacacaaa
      181 aagggcaaca ctcggaaaca tgttacccta atgagcccgt taaaaagacg gtgaccttat
      241 attttattta aaaatagcct tcaaaatgcc ggtcaaagcg aatagaaggt tattttttat
      301 ggtcttgagt actaatcaat actaaaccaa tgattgttaa tacaaccatc agcatttcaa
      361 aatcagacat gctagtcctt tctaggagta acaactataa ccataggcat caccccttta
      421 ttaagagatt agccaccatc tatccaactt gctcaataaa tattataaca tgcacacttt
      481 taaacagtaa ttcatagtat aagttttttc atttcttaat caactcgata aacagttgag
      541 tgacataccc ttgtctaact agctatttct tcccctgtta tcgtttgttc atcatataaa
      601 aatttaattt ctcatttttt attctctggt aaagaagagc accaaccttt tcggctgata
      661 tgctcccttc aaaatagaca ttgaaaaatg aaaactttga aagagaaatc gtcagtctat
      721 tattgataag tattatccta tcctcgaaaa attactttca gacactagtg aacaaaaatt
      781 ttactataag cgtatacttt ggcaatattc gaaggaaaaa catggattaa cctgtgcata
      841 ttatacattt agagcttata tacttaaaca tgatgaattt actccttata ttatgaaatg
      901 ttatcaatag atgtcgccta aagatacaac aagattcgag acaaaacctg atcatcaagc
      961 acagtttgac tggaaagaaa gcattaactt taaaacgaaa gataatcaaa ggattccact
     1021 atatataggt gtgctactac tttattattc tcgttttata atcatgcata acaatgagta
     1081 aatcaagtga tgccttattt aattcattaa cacaatcgtt tgagctaata gaaggtgtat
     1141 ataatgaact tgtaacggat aacttgaaaa tagtcatgta taagccaaga aaagaaagta
     1201 caagtggtcg aataaatcgt agatttaaat aattcactga tggttttaaa tttcaagtaa
     1261 aaccttgtat ttccggcctc cctagaaaaa gataaagttg aatcaataac aaaaatatta
     1321 gatgaaatta atgcttacca agacgaattg catttggaag agattcctaa gtttttttaa
     1381 tcttaatgaa cgatttaact actaagttca tactagtaca ggtaaattgc ctattactgc
     1441 ctaagagaaa gaaaaaattt tcttacaacc actacagaat gtgaatgtaa aaaactcata
     1501 taaagtgaaa catatcttac ttatggaatg aaatgtttga ttatcccaaa atcacaaatg
     1561 cgattttgga gttgttaggg gctctaacgc aaatacaccc ctacttaaaa taacggtagt
     1621 tgttatagta tacttaaatg cataaaccat ataaaaggtg atgctaatat gacatacggt
     1681 tcaattgtag ctatactctg tgccctattt gtgattttct tcattccata tatggagaag
     1741 aaagacaaaa agaagaaata acactagtat ctggattact agcatacaaa ggagcagtaa
     1801 gacattttct aatagaaaat aactttactg ctcctttgta aattactctt caactattgt
     1861 aaattaaata ataaataact gccgatgtta ttaatcagct aaaaaatgct ttgctaaata
     1921 ttttgctgcg ccatcctctt cattcgtgta tgccgttaca tctgaagtta atgcttggat
     1981 ttcagggcgt gcatttttca tagcaactgt ataatgacca aattcaaaca ttgctctatc
     2041 attgtcgcta tctccaataa ctaacgtttc ttcttgatga ataccaaaat gtgcaatcat
     2101 ttctttaatg cctgtacctt tatcagtttg ataagccatt gtttccgcat taaatcttga
     2161 tgaatttgaa acactaatct gtagttgcac atgattttgc tttaattcat ctctaaatgc
     2221 tgttattttt tctaaattag aactgaatag ataaattttt gaatattcac cttcaggaaa
     2281 cttagttacc caatctatct taccagcaag cgcatcttgt cttgaagacc attcactatg
     2341 acttacgcca ttaataggat cttgactacg aatcatatct cgcatccatg tttcatcttc
     2401 ttttaaagaa actctattac cttcaaaagg aaatacctca taataaattt gttggcgctt
     2461 agctaaatta gtaatttgtt gcacttgagc caatgataaa ccatgcttga agataatttc
     2521 tccatctact tctccaattg ttccatttga actaatgatg ccattaaccg caaaatcttg
     2581 aggtacaagt tgatgtattt cagaatgcga acgtcctgtt gccaaaaata ctttatatcc
     2641 tttctctctc aatcgattaa tgacatcttt cgtatacggt gatgcttgat tattttcatg
     2701 taaaattgtt ccatccatgt ccaaaaatat tgcttttacg ttatccatat tgctctccac
     2761 tgtccatttt atcttaatta aattttgtca ttattagtta gtatttgcaa aatatttact
     2821 ctaacattgc catagaaaat cgattattcc atctttgaac tgctacattt ttctggaaaa
     2881 atcgtgacat gttttcagat gtcaaaatat catctattaa tccttgttgt acactttccc
     2941 catcttttaa tagcaaaatt ttagtaaagt tactagtaat ttcttcaata aagtgcgtaa
     3001 cataaatcat cgcaagcatt ggatatgaat ctgacaatga gtcaagtata cttaacaacg
     3061 attctcgtgc aataaagtct aaaccagctg ctggctcatc taaaattaaa acctggggtt
     3121 gccccattaa agctcgcgca atcatcactc gttgtttttc acctgtagat aaataaccaa
     3181 tatattgttg cgctttagca gacattccaa ctaatttaag taattgatgt gcttcattac
     3241 gtacttcttc atcaatatct tgataaacac caattgattt aaaagcaccg cttatcacca
     3301 catcgattac tctttcaccc tcttgaaact tttccagtaa actatgagat acaaaaccta
     3361 tatgttgtcg tacagtctct gcagaatacc ctaccttgcc tggcatttta ccgaaaaggt
     3421 taacagttcc agatgttgca ggctcatacg catttaaaat atttagaagt gttgtcttgc
     3481 cagcaccatt caacccatat aatatccatt tatcaccttt agcaatttgc caagaaatct
     3541 tttttaaaat tgtttttcct tgcttcattc gcccaatttg atctaattga attaacatgc
     3601 gaggtcaccc ctttattgct actccatact tctcataaaa tcatattaac ataacaccct
     3661 taattgtcag actatttaaa taaataaaac acttcatttt tacgcatttc tgccaaatta
     3721 agatgaagta aagctaagtc gacctaaaaa agcacccttc tagtcgatta atctaaaagg
     3781 gtgccatata ctttaatttt aatacatgat tgattctaaa aaagtgaatt attccacagt
     3841 aactgattta gcaaggttac gtggtttatc aacatctaaa tctctgtgta atgctgcata
     3901 gtatgaaatt aattgtaatg caaccactga tactaatggc gttaacaatt catgtacatg
     3961 aggaatgaca taagtgtcgc cttctttttc aagaccctcc atagaaataa tacatggatg
     4021 tgcaccacgt gctactacct ctttaacgtt accacgaatt gataaattaa ctttctcttg
     4081 tgttgctaaa cctacaactg gtgtaccttc ttcgattaag gcaattgtac catgtttaag
     4141 ttctccacca gcaaaacctt ctgcttgaat gtaagaaatt tctttaagtt ttaacgcacc
     4201 ttctaaactt acgttatagt caatagtacg tccgataaag aatgcattgc gtgttgtttc
     4261 taagaaatct gtagcaattt gttccataat tggtgcatcg tcaacaattg cttctattgc
     4321 tgttgttact tttgctaatt ctctcaataa atcaatatct gcttcacgac catgctcttt
     4381 tgcaacgatt tgagacaaga ttgataatac tgcaatttgt gcagtatatg cttttgtaga
     4441 tgcaactgcg atttcaggac ccgcgtgtaa taacaatgtg tggtctgctt cacgtgataa
     4501 agttgaacct gcaacattag tgattgttaa tgatttatga cctaatttat tagtttcaac
     4561 taatacggcg cggctatctg cagtttcacc tgattgagaa atataaacga acaatggttt
     4621 ttcagataat aatggcatgt tgtagacaaa ctctgatgca acgtgtactt cagttggtac
     4681 gccagcccat ttttctaaaa attctttacc tactaaacct gcatggtagc ttgtacctgc
     4741 tgcaataacg taaatgcggt ctgcttcttt aacatcattg atgatgtctt gatcaatttt
     4801 caagttacct tctgcatctt gatattcttg aataatacga cgcattactg ctggttgttc
     4861 atgaatttct tttaacatgt agtgtgcata aacacctttt tcagcatctg atgcatcaat
     4921 ttcagcaata tatgaatcac gttctacaac gtttccatct gcatctttaa taataacttc
     4981 atctttttta acaataacga tttcatggtc atggatttct ttatattcgc ttgtcacttg
     5041 taacattgca agtgcgtctg atgcgataac attgaaacct tcaccaacac ctaataataa
     5101 tggtgattta tttttagcaa catagattgt gtctttgtct tcagcatcta ataaacctaa
     5161 tgcatatgaa ccatgtaata atgacacaac ttttgtaaat gcttcttcag ttgaaagtcc
     5221 ttgatttgaa aagtattcaa ctaattgaac gataacttct gtatctgttt ctgaaatgaa
     5281 tgatacacct tgtaagtatt caccttttaa ctcttcatag ttttcaataa caccgttatg
     5341 aactagagta aaacggccat ttgatgattg atgtggatga gagttttcat gattcggtac
     5401 accgtgtgtt gcccaacgtg tgtgaccgat tccaacaggt ccatcaaaat cgctactatc
     5461 agcaacttta cgtaattctg caatacgacc tttttcttta aatacagttg tattatcatc
     5521 atttactact gcgatacctg cagagtcata acctctgtat tctaattttt ctaaaccttt
     5581 taataataat tctttggcat tatcatagcc aatataacca acaattccac acataacgac
     5641 attttcctcc atattggaat agtacgcgta aattatgatt tattgccgat aatttagatt
     5701 gacaatctgc tttcataata taaataggaa catgctatca tcgcattcat ccataacaaa
     5761 ttaagcatag ttatttttac aactatacaa attgctcaca ctgtactttc catattaata
     5821 ttttttatat tcaatttctg gcgatcttat taactttgtc cattaagtca ccctaatgtt
     5881 ttacttaata agctaacgaa tgagccacat ccgggatagc atccgccgat ctattcgatc
     5941 actatcctcc tcgtctacaa atacatatat tgcactctat aaaggccact catatattaa
     6001 cctttaatct tcaaatacaa atatttattt gcacaggcgc tttaactgta ctgccgaact
     6061 ttcccccttt ccattaatca ttattgtaca acggtgttgt tttgttttgc aaatattttc
     6121 acaataaaat tttaaaaatc ctaaaacaat ttttttgttt tactttttca aaatatctat
     6181 actgtcacat tgatgacact ttatttaatt ttgtcacatt tattttgaca aagttgattt
     6241 ttgtttatat tgagtaacaa gtaacctctc tatacactat atatagtcac atatattaaa
     6301 aaagaggtgt aaacatgtca caaactgaag agaaaaaagg aattggtcgt cgtgttcaag
     6361 catttggatc attcttaagt agtatgatca tgccaaacat tggcgctttc atcgcttggg
     6421 gttttatcgc ggccattttt atagataatg gatggttacc taacaaagat ttagctactt
     6481 tagcaggacc aatgattact tatttaatcc cattattgat cgcatttagt ggtggtcgtt
     6541 taatttatga tttacgtggt ggtatcatcg cagcaacagc aactatgggg gtcatcgttg
     6601 cattacctga tacaccaatg ttacttggtg caatgattat gggtccactt gttggttggt
     6661 taatgaagaa gaccgaccaa ttgattcaac ctagaacgcc gcaaggtttt gaaatgttat
     6721 ttaataactt ttctgctggt attttaggat ttatcatgac gattgcagga tttaaaattt
     6781 tagcaccact tatgaaattt attatgcata ttttatcagt ggcagttgaa gcattagtac
     6841 atgcacacct acttccactt gtaagtattt tagtagagcc tgcaaaaatt gtatttttaa
     6901 acaatgcgat taaccatggt gtattcacac cgcttggtgc agatcaagct gcaaaagctg
     6961 gtcaatcaat tttatacaca attgaatcta accctggacc aggtttaggt atcttacttg
     7021 cttatatgat ttttggaaaa ggcactgcaa aagcaacgtc atatggtgct ggaattatcc
     7081 acttcttagg tggtattcat gaaatttatt tcccatatgt attaatgcgt cctttattat
     7141 ttatcgctgt tattttaggt ggaatgactg gtgttgcaac ttatcaagca acaggatttg
     7201 gatttaaaag tcccgcgtca ccaggttcat ttatagttta ctgtttaaat gcacctagag
     7261 gtgaattctt gcacatgtta ctcggtgtct tccttgctgc acttgtatca ttcgttgtag
     7321 ctgctttaat tatgaagttc actagagaac caaagcagga tttagaagct gcgacagctc
     7381 aaatggaaaa tactaaaggg aaaaaatcaa gcgttgcttc taagttagta tcttctgata
     7441 aaaatgttaa tacagaagaa aatgctagtg gtaatgttag tgaaacatct tcatcagatg
     7501 atgatcctga agcgctattg gataattaca acactgaaga tgttgatgca cacaattaca
     7561 ataatataaa tcatgttatt tttgcctgcg atgcgggtat gggttctagt gcaatgggtg
     7621 caagcatgtt acgtaataaa tttaaaaagg cgggcattaa tgatattaca gttacaaata
     7681 ctgcgattaa tcaattgcca aaagatgctc aattagttat cactcagaaa aaactaactg
     7741 atcgtgctat taaacaaaca ccaaatgcca tccatatttc agtggataat ttccttaatt
     7801 caccaagata tgaagaactt ttaaataatc taaaaaaaga tgatcaagca taataattaa
     7861 ataaattaaa aaatggagga taccgccatg ttattgagta cacgtgaaaa agaaatgata
     7921 gccctattga ttaagtacca cggtcaatat atcactatac acgacattgc tcagcaactt
     7981 gcggtgtcct ctcgtactat tcaccgtgaa ttaaaaggtg ttgaagcata tttaacttca
     8041 ttttcattaa ctttagaacg cgcaaacaaa aaagggctac gcattgctgg cacagattct
     8101 gatttaaacg atttgaagca atcgattgca caacatcaaa ccattgactt atctgttgaa
     8161 gagcagaaag taattattat atacgctttg atacaagcca aggagccagt taaacaatat
     8221 agtttagcgc aagaaattgg cgtttctgtc cagactttag caaagatgtt agatgattta
     8281 gagcttgatt taaataagta ccaactatct ttatctcgaa agcgtggcga aggcatttac
     8341 ttggtaggta ctgaatcaaa gaaacgtgaa tttttaagtc aattaatggt gaataactta
     8401 aatagtacta gcgtttattc agtaattgaa aatcattttg tctttcattc attaaatcaa
     8461 atccacaaag actttgttga cttagagcgc atttttaatg ttgaaagact attaatggac
     8521 tacctaagtg ccttacccta ccaacttacc gaatcaagtt atttaacttt aactgtccat
     8581 atcgtgctat ccatttcacg tataaaaaat ggtgagtatg tcgcattaaa cgatgatatt
     8641 tatgattctg tacaaaacac atttgaacac aaagtagcaa gcgaacttgc tgataaactt
     8701 ggtcaaatat atgacgtcac gtttaatcag gcagaaattg ctttcattac tatccattta
     8761 cgtggagcta aacgaaaaaa tcttaatgat acatcattaa ataatcgttg tgaagaaaac
     8821 aaaattaaag agtttgttaa caaagtagaa atgatttccg gtatgacatt tgcagatttg
     8881 gatactttag tagatggact gacgctacac cttaatcctg caatcaatcg tttgcaagct
     8941 aatatcgaga cctataatcc gttaacagac atgattaagt tcaaatatcc aagactattt
     9001 gaaaatgtaa gattagcttt aaatgattgt tggcctgatt tgatttttcc agagagtgaa
     9061 attgctttta tagttttaca ctttggtggc tcgattaaaa accaaggtaa tcgattttta
     9121 aacatattag tcgtttgcag cagtggtatg ggaactagtc gtctattatc aactcgtcta
     9181 gagcaagttt ttagtgagat tgagcgtatt acacaagcat cagtcagcga tttgaagtca
     9241 ctagatttaa gtcaatatga tggcattatt tctactgtga atttagacat cgactccccc
     9301 tatttaacgg taaacccatt attaccagat agtgatatca gttatgtcgc acagttttta
     9361 aatacaaagt ctacgttcca agagacgcat gataaatcat caaacatgat tgataaggat
     9421 gatgttcatg ttgaaacgaa agatattgat ggcaatacat cttttgaaaa tgaacaaact
     9481 tcatacttaa cttcagtttt cgaaaaacat ttaagtgacg aaaaatcaga acaattaatg
     9541 catcatatgc gttcgggttt aactttgctt gattcagtga aaatagttag taccgaagtt
     9601 aaacagtggc aaacatatat cgcagattat ctatatcaat gcgatgcaat aaacgatcca
     9661 acgtcattcg ctgaactact agagcaacga ttgattgaca atccaggatg gatattaagt
     9721 ccatatcctg ttgcgatacc acacctaaga gacaatatga ttaaacaccc tatggttcta
     9781 atcacagttt tagaagaacc gttaatattg cctagtattc aaaatgacaa tcaaacaatt
     9841 aaatatatga tttccatgtt tattcctgac aatgatttta tggcatcact ggtaagtgac
     9901 ttgtccgaat ttttaagttt gaaattagaa tctattgata cttttatgga aaatccacag
     9961 gaacttgaaa cattattaag aaacaaattt ttagaacgaa ttaaaaaaca atttatttag
    10021 gagtgtacaa aatgagcgaa ttatttagta atgacaatat ctttttaaat gtaaatgtta
    10081 acagccaaaa tgaagcaatt gaaaaagcag gtaaagcctt agttgatagt ggtgctgtaa
    10141 cagatgctta tattcaagca atgaaagatc gtgaacaagt cgtatcaaca tttatgggaa
    10201 atggcttagc aattcctcat ggcacagatg aagctaaaac aaatgtgatt cactcaggtt
    10261 taacattatt acaaatccct gaaggcgttg actgggatgg cgaagtagtt aaagttgtcg
    10321 tgggaatcgc tggtaaagat ggcgaacatt tagacttgtt atctaaaatt gcaattacat
    10381 ttagcgaaga agaaaatgtg gatcgtatcg ttcaagcaaa atctgcagaa gaaattaaac
    10441 aagtattcga ggaggcagat gcataatgaa agcagttcac tttggtgctg gtaacatagg
    10501 tcgtggtttc attggttata ttcttgcaga caacaatgtt aaagtaacat ttgcagacgt
    10561 caatgaagaa atcattaatg ctttagctca tgatcatcaa tacgatgtta ttttagctga
    10621 tgagtctaaa acaacgacgc gcgtgaataa tgttgatgca attaattcaa tgcaaccttc
    10681 tgaagcgttg aaacaagcaa ttctagaagc tgatattatt acaacagctg ttggtgttaa
    10741 catactacct attattgcta aatcttttgc gcctttctta aaagaaaaaa caaaccatgt
    10801 taatattgtt gcttgtgaga atgctattat ggcaactgat acattgaaaa aagcagtact
    10861 tgatattact ggccctcttg gtaacaatat tcattttgct aactcagcag ttgatagaat
    10921 tgtaccatta caaaagaatg aaaatatatt agacgttatg gttgagccat tttacgaatg
    10981 ggttgttgaa aaagatgcat ggtatggccc agaactaaat catattaaat atgttgatga
    11041 tttaacacca tatattgagc gtaaattatt aactgtgaat acaggacatg catatttagc
    11101 gtatgctggt aaatttgcag gtaaagctac cgtattagac gctgtaaaag atagttcaat
    11161 tgaagctggc ttacgccgtg ttttagctga aacaagtcaa tatattacta atgaatttga
    11221 ttttactgaa gcggaacaag ctggttatgt tgaaaaaata atagaccgtt tcaacaattc
    11281 ttatttatct gatgaagtga cacgtgtcgg acgaggtacg ttacgtaaaa ttggccctaa
    11341 agatagaatt ataaaaccat taacatatct ttataataaa gatttagaac gcactggttt
    11401 attaaataca gctgcattgt tattgaagta tgatgataca gcagaccaag aaactgttga
    11461 gaaaaataat tacattaaag aacacggttt aaaagcgttt ttaagcgaat atgctaaagt
    11521 tgacgatggc ttagccgatg aaataattga agcgtacaat tcactttcat aatttattga
    11581 gctttgtttg aaacaagaag tttccaacgt tattcgttaa caatcagtaa taatgtagta
    11641 gttccgttga attaacaata ttaaatttct gaacataaaa aaatactccc ttcaacatag
    11701 acacttaact tgtgttatgt atgaaaggag tatttttgcg ttaataattt gttttatttt
    11761 cgagccacag ccacctattc aatggctatt ggtcattact aaaacaaatt catattaact
    11821 gttagacttg gttacttagt aaggaatatt tccctatgaa ataactagat gttcacattc
    11881 ttgaataaat tttattcttc agtttgttgg tctttcttag tgaatcttct aattaagaat
    11941 gccatacctg cacctagagc taattcagca tatggtaaat cgtcattatg tgacatacca
    12001 gtatctggta aagttttagc ttgttgttta ggcttattaa cttttccttg ttggtctgat
    12061 tttgcatttg ctttgttttc ttcagtctta gttacattaa gcatatcttg atgcatacta
    12121 tgactaccat tagaaactgt tgctggagat gcattggcac cgtcgttttg cgtagcttta
    12181 ttgtttgcaa ctgggccaac tgatgtttgc gtatcattag tttgcatatc tttagcttta
    12241 ttgtttgcaa ctgagccaac tgatgtttgc atatcattag tatctgctgt tgccgtatca
    12301 tctttttggc taacattagt tgaagtcatt ttttcttttg cttcagaaga tgcagatgtt
    12361 gatggtttat tcgaaacttc agtatcagct ttgcttggcg atttatctgc ttcgttagat
    12421 gcaacgttag tttcagactt aagttgtcct gcatcagttt gatttgacgt actttcctct
    12481 ttatcttttg atgtattaga aggtacattt ggttctgtta tgtctgctga aggcaatgtt
    12541 tcagttgttg attcaaccat actttgattt gttgaatctc taccatcttt ttctgcctta
    12601 gctttatttt cagattttgg ttgtgcaacc ttgccattag ttgattgaga ttcagcacta
    12661 ttatttactt cagcattttg ttttgaatca tttacagatg cattatcttt actatcagca
    12721 gatgatgctg cttctgtgct cgcagttgtt ggagccgtcg ttgttgatcc tgttggtgca
    12781 ttctcgtttg ttgctgtagt tgtactattg ttatttgttg tgctttctgc tggcgttgca
    12841 ttatctgttt ctgttacagg tttatcagtt gtgccgttat tagttgattc tacttctggt
    12901 ttactagtta catcgttatc cattgtcgga ctgtttgttg atgcatctgc actagaattg
    12961 ttattagctt gcggtttatc ctttgcatca tcagttgctg atgttactgt tgtttcacct
    13021 gttgccgcat cactattatt tggtgttgtc ggagaagcgt ctgctttgcc attatctgtc
    13081 gtctcagata cgttaggttg tccagtgttt tctggtgttg cattagcatt tgaatttgct
    13141 gttgcatcat tattatctgt accattatta gtatcattag catctggatc attctgaggc
    13201 acaatcgctt caattgcagg tatcgttaca ttttgtaatt cagcaacctc tgtatttgtt
    13261 tgtgttttat ctaatttatc agcaaatctg tcaaaatatc tacctaaatc tgtacgtgca
    13321 atttctttcg ccgatgcatc tgcatctgca tttttaatta tttctatttg cttgttaacc
    13381 acttctctaa ttgcttccaa agcatttttc ttaactactg gattaatacg ttgtgcttta
    13441 agttgttcaa gcgcactatt tttgacagta gcgatttctg catttgtagt ttgatcagaa
    13501 atatcttcag ttgcttttga taaaatgtct tctaaagcat tcgtaaacgc ttctttttct
    13561 tcagttgtag catcagcgtc gacatttaca cctgcttcaa tctggtctag tgcagtttct
    13621 aattcttcga tagctttttg tttttctgtt gagtcgattt gaatgttatc aaatgcttca
    13681 agtccttgag ctttcgcttt ttcaacttca gcagttgttg ttgcatcagt aataccttgt
    13741 ttagcttgat ctgtaatttg tttaatcatt gctaatgctt cagttttttc ttcagcagtt
    13801 aactggtcac tttgatcaat agattctttc gtatcttctg ctttaacttc aatagcttgg
    13861 ttcgctttag gtttaacagt agcatctact tgaatagcat caattgctgc tttaccttgt
    13921 gttaatgcat catcaacgtc accattatcc acaccattat taatgctttc taatgcagtt
    13981 tgaacatttt ggtcaacttg cttaattgct tattgctttt catcttgtgt tgcattagtg
    14041 tcagctgaaa tattattttt cttctgatct gcataagcat ataaatctgt tgtagctgat
    14101 tcttttttac ctgttggaat tgtgtaatcg ttaatattat ctaagtcatt atgaatttga
    14161 acttcaatgt catctttaga agtagcttga ttaacatttt gatccgcagt ttgttttaat
    14221 tcagcaagtt tttgtttcgc ttcagcaatt tcacttgaag tcgatgcgtt agagttatcc
    14281 gcttcgttta ctttagcatt atatgcatct tcaattttag ctaaagcatc ttttttgtac
    14341 tcactaaatg ttttaactgc attaatttta gcttttcctt ctttaactgc attatcaaca
    14401 tattcatttg ttgatgattg atcgacattt gttttcgctt gatttaattc agtatcaact
    14461 tcttgttttg catcattaat ttcttgttgt gatgcatttg gtgtttgttc tatttgtgtt
    14521 ttcttatcag ctgcagcttg atctaattct tttttacctg ctggtttctt aacaggattt
    14581 gcatgaagtt gttgaacttt ttgtactgct gtatctttag cagtagttac atcacctgta
    14641 gtagttgctg cattaatatt atttaaacct tcttcatatg ctgctctaac tggtccaata
    14701 tcgttacctt tttcttcatt agtagtctca ttattattaa gtatttcagt tattttattt
    14761 tgcatttcag ttagcaattc cgctcttgca ttcgttttaa catctgttga tgcttgaatt
    14821 gggtcgatgg cttgaattgc attatctttt gcagtgttta cgtcatcaat tgactgtgca
    14881 ttttcaatat tttgattacc ttgtgttaat tgtgcgtcta cttgttgatt tgcttgttct
    14941 ttttcttcag tagtcgcatc tgcagtttgt gcaataagcg ctttttgttc gtttgctttt
    15001 gttgctaatt catctttcgc aacatcttta attgttgtat ctgcagtaat accttgaata
    15061 tcagcaactg cttgatcttt aatttgcgta acatcattag ttgtttgtgc atttaagata
    15121 tcttgatacg ctttttcttt agcttttaaa actaaatctt ttgctgcatt tttctcttca
    15181 gttgtagcac cagttgtatt atcaattgct tgattttgag ttgtcacagc ttgatcaaca
    15241 tcatttttag catttgattt aaccgatgtt gctggttgcg tgctttgaat tgaattcttt
    15301 ccagcgtctt tcgcctgatc tacaccatta tcatcagttg cagctgtaat attatttttc
    15361 gcgtctgtaa ctgctgttgc taattgttga atagctgctt ctttttcttc agttgtagcg
    15421 ttctgatcat tattgataac atttgtttgc gttgcttgta attgatcaat ttcatcttta
    15481 gccgtttgtt tcttcacaac ttttggtgtt accgcattaa tcgctgcttc tgcatttgtt
    15541 ttagcttcat caacttgtgc gttagtagtt gctgctgcaa tggcttgaat tgctttacca
    15601 ttttcagtat ttgcttctgc atcagctgct tgtttttctt catctgttgc atctggcgta
    15661 gcttgaatct cttgcaattt gttatttaaa attgctgtga tttcattacg tgcagttgct
    15721 tttttattaa ctgttggtgt tacttgatca atactagttt cacctgtcgt tttcgcttgg
    15781 tctacatcat tttgactatt agcagcttca atgttgctat ttgcttgtgt cacagcatta
    15841 tctacgtccg cattagccgc tgcaatttct tcagcagtaa tgtcttgcgt ttgagcgatt
    15901 gctgttttac gttcattcgc tttcgtagca attgcttctt tcgcattatc tttagttgtt
    15961 gttgctggct gaatcgcttc aattttagca atttctgcat ttttagccgc ttcaacttcc
    16021 gcatttgtat gtgctgcatc tattgctgca tcagcagtcg ttttttcagt ttggacttgt
    16081 tgtttcgcag ctgctttttc ttctgttgtt gaaccgttat tagcatcaat tgctttttct
    16141 tgagcttgta ctttatctgc aattgcttgt ttcgcttgag gtttaacatt tgcatcaggt
    16201 gtaatggctg cgattgtagc ttcatttgta gtttttgcat tatctacatc tgtatttgct
    16261 gcagcattat ctatatcagc gtttgcagac agaacagctt gatcaacttt ttctttcgct
    16321 gcttgttgtt cttcagtagt cgaatcattc attgcttcga ttgctgtttt acgttcactt
    16381 gctttttgag caatttctgc tttagcatcc gatttcttag ttgtggcagc ttgaacttga
    16441 ttaattgcag caataccatt gtctttagct gttgttacag cactgtttgt atttgcagca
    16501 tcaagatttg ttcttgcttc ttgcttttta gtatctaatt ctgtatatgc agcttgtttt
    16561 tcttctgtag ttgaagcatt gctattttga atttcttgtt tacgtgtatt atatgcgttt
    16621 tctacttctg tatcagctgc agatttaact tttgcttgtg tttgaattgc attgatttta
    16681 tctaatactt ttgattttgt atcagcaact tcctgttttg atttaacaac ttggatgtca
    16741 tgtaaacctt gcttttgagc tgcttctact gctgcatcag cttctgctac ttcttcattt
    16801 gttgcatttg aaactgtagc attttgagct tttctagctg ttgcagcttg tttaacttca
    16861 ttataggcat ccatttttgt ttgcgtagag tcagtaatat tttcaatttt tgagatttct
    16921 tcgtttttaa ctctttctaa atcttgtgca gtcgttgtcg cttcaattgc tttaacacca
    16981 gaaactttag ctgcattaat acgttcaata gcttccgcta cttcttcatt tgttgtatca
    17041 ggatttaaag gtgcttgatc aatttgtgct tgaacaactt cgtcaaattc ttcaagagct
    17101 gctgctttag ctgttgtagc aggtgtagtt tgattaatat cattgattgc ttttgtttta
    17161 atggcttcta cttgtgcatt tgttgttgct gcatcaatat ctttaattgc ctttgtttca
    17221 attttaccaa ttttatcatt tgctacatct ttttcatctt gtaatgatgc atttgacttt
    17281 ttaatttgtt gtttacgagt tgtaactgct tggataatat cttgtttcgc ttgaggttta
    17341 actgttggtg taatcacatc ttgttctaac actgcgatac cattatcttt ttcagttgtg
    17401 acaccttgtg atgttgtttg tttattaatt tctgcgattg cattattttt atcattgtta
    17461 attttagtca caagcgctgc aacttcatct tgcgtaactt ttttactttg ttgtgctgct
    17521 gtaacctttt cttgagcttt agtatctaat tcagcaattg ctgcttgatt atcaattaat
    17581 gcagcttgta atttagttac taaaccatca atatccgctt gagatgcacg atttgcagtt
    17641 tcaacatgat tcgcatcttc atttaaaata gtatctgctt gttgtttaag tttattgtat
    17701 tctgcaattg atgctgttgt gtaatgacta ttatcaactt gtgagtttac ttgttgttgc
    17761 aacgcatctt tgttcatttc aaccgctact gaaaatggat ctgcagttag tgtaacttct
    17821 ggttgcgcat tattaattac aacatctgaa gcagtacgat atgttaattt ttcattaaaa
    17881 tcaatatttt taggtgtatc gatattcgca acattaactt tatatgataa ttttaaagat
    17941 ttatctggga ataaaacttc tttagtgtgt gtaccacgtg ccgttgtcac accttggctt
    18001 gtaaatgtaa ctttatttgc agtttgatca taattaacag tcatattttt caatactgta
    18061 ctgtcttcat taccattagg gaatgttgta gttaatgaat cattaatgta agttacacct
    18121 tgtggtaatg ttacttcata tttaaattga tctgctttca atgaagcacc aaaattacca
    18181 ttgttaccga tttcagtatt aattgtaaat tctttattat ttgttgcttt cggtgctaaa
    18241 tctctatttt tgacatataa atgtgatcct gagaatagac ctacattatc aatgaatgaa
    18301 tagtatttat aaccatcttt atttgttgta attcttgaag catcagcaac tgctgtattg
    18361 tcaggaataa attgtaaatc taaacgatca gcatctgttg gtactttaaa taagtgtgca
    18421 gtaccagtct tagctacatt tacagttgct aattcagtat ttttcttagc atctttaata
    18481 actaaatttg ttgcaccttg cttactattc gtttgcatag tagtaaagtt gataattaat
    18541 tctgaatctg gttttacatt aacagttttt gaaataccgt taaagttacc atgatctgta
    18601 gaatcatttg cattcacacg acctaatgca gctacatttc ccctagcttg atagttctga
    18661 gggttattct tatcaaacat atcgcttcgt cttaattctg agttaacgaa accaatctta
    18721 ccgttgttaa ttaatgaata accatttact ttatctgtaa cagttacagt tggatcctgt
    18781 ctattctcat ctgttgatat ggcaggatca tcaaacgtta atgttgtatt aatactgcct
    18841 tcaccagtat tgctagcatt tggatcttga gtttgtgcgt ttgctgctac aggtgctgct
    18901 ggttgcgctg ctggagcatt cgctggctgt gtttgatttg ccggtgttgc ttggttattt
    18961 ccttgtcctg cttggtttgc cggtgctgct tgatttccag gttgtgcttg tgcagcgtta
    19021 tttggatcag cttgattacc ttgtccagct ggttgtgctg tacctgctgc cggttgtgct
    19081 gcgcctccta ctggttgtgc tgcgcctcct gctggattag cctgtccacc ttggtttgct
    19141 ggttgtgctg ctggttgtcc ttggttcgca ggtgcagctg gttgtgctgc aggattagct
    19201 tgagcaccag catttgcgtt aggctgtgca ttggcatcag ctggttgtgc tggttgattt
    19261 tgtgcaggct gattttgctc tgctgcagac gctgttgtcg ggttaataga tataaatgta
    19321 acagtggcaa ttaaagctga aaaaataccg acattaaatt ttctgatact aaatttttgt
    19381 tgtctgaata aattcattaa gtcatcctcc tggttgatta ttctcgctgt taaatgattt
    19441 cacttaatca actgttaaga taagtagtag catctgcgtt aaaaacacaa agcaactcta
    19501 tctaattaaa attaatttta tcatcattat atatttagta ccagtgtatt ttatattaca
    19561 tatttattac tttgttttta ttttgtttat atcattttac gtttgtacta taaattattt
    19621 ctagaaacac aaaaaaccga tgcatacgca tcggcttatt tgtaatacag tatttatggt
    19681 atttatttat ctaatcccat tttatcttga accacatcag ctatttgttg tgcaaatctt
    19741 tcagcatctt catcagttgc tgcttcaacc atgacacgaa ctaatggttc tgttccagaa
    19801 ggtcttacta aaattcgacc ttctccattc atttctactt ctactttagt cataacttct
    19861 ttaacgtcaa cattttcttc aacacgatat ttatctgtta cgcgtacgtt aattaatgat
    19921 tgtggatatt ttttcatttg tccagctaat tcacttagtg atttaccagt catttttatt
    19981 acagaagcta attgaatacc agttaataaa ccatcaccag ttgtattgta atccatcata
    20041 acgatatgcc cagattgttc tccacctaag ttataattac cgcgacgcat ttcttctact
    20101 acatatctgt cgccaacttt agttttatta gatttaattc cttcttgttc aagcgctttg
    20161 taaaaaccta aattactcat aacagtagaa acaatcatgt cattattcaa ttcttgattt
    20221 ttatgcattt cttgaccaat aataaacata atttggtcac cgtcaacgat ttgaccattc
    20281 tcatctactg ctatgattct gtctccatcg ccgtcaaatg ctaacccaaa atcactttca
    20341 gtttcaacaa ctttttctgc taatttttca ggatgtgtag agccacattt ctcattgata
    20401 ttatatccat caggactaca tccaattgtt tcagtatctg cttctaaatc accaaataag
    20461 aatggcgcta atgatgatgt tgaaccattt gcaccatcta aaacaatttt caaaccttca
    20521 aagttaacat ctactgttga ttttaaataa ctcaaatatt tttgtgcccc ttcaaagtaa
    20581 tctgaataat gtacaatatc attaccaact ggtcttggta attctgggtt ttcttgatcc
    20641 aataatgctt caatttcatt ttcttgttca tctgatagtt taaaaccatc tgatccaaag
    20701 aatttaatac cattatctgc aactggatta tgagaggctg aaatcattac acctaactct
    20761 gcacccatat cgcgtgttaa atatgcaaca cctggtgttg aaataatacc taatcgcatc
    20821 acttctgcac caattgaaat caaaccagct attaatgctg attctaacat ttcacctgaa
    20881 actctagtat cgcgacctac aagtacacgt gggtgttttt cacctttatt atgtgctaga
    20941 acatagccac cgtatcttcc taatttaaat gccaattcag gtgttagttc ttggtttgcg
    21001 acacctctta ctccgtctgt accaaaatat tttcccattg tttactctcc tttaatttag
    21061 ctatttattt tacatttata taagccttcg tttcacttgg ttgtgcttta gtgacatgtt
    21121 ctggtaaatt gatttttaca gtcttttcag ttgattctga aataccatct aaatctactt
    21181 ctgcatcaac ttcgcttata ttttgtaagt catctcgact accgaagatt tcaatttctt
    21241 tatcttctaa atcaatcgaa cttaactctt tatcatctgc taaactacct ttctgtttaa
    21301 catttacttt aacctttttg ctaaaaggct ctactttaac ttgtaaattc acttcattag
    21361 gttgaatcga tacatttaat ttattcagtt ttttatcaaa agccgttact tctgcgacat
    21421 cttttgtgtc accattaatc tttttattag ttttaaaagt ggcttttaaa taagcgatat
    21481 cattcaattg ttcttctcca cctgttactt taactgtttg tggtgaaact tcttgctttg
    21541 taattttata aagtggatca atatcactct gacttacatc aggttgaact gtcatctttt
    21601 tagttacttt gttttcaagc gtaatatttg ctaatttagg ttttacagaa taatgaatgt
    21661 catcagctaa ccctttaact tgatacttct cttgatattt tccagcttta gcatttgata
    21721 aatcaatcac tactcttaaa tcttctggat tttcaatttt tattatcttt gattgtggtc
    21781 ctgaaatagt cacattaact gtttcaggcg cttttgttaa atgcaaatct ttagtgttat
    21841 aaagaatttc aacgggtaca tcttgaatcg ttttactaga cttttgacca agattaccag
    21901 tgttaaagat atttccaaaa acattgttaa cagataaaaa gaaaaacaat gccaaaagaa
    21961 aggcaataaa tctcaagccc catttacttt ctagcatatt atttcacacc tttcttttga
    22021 aagcgtgtgc caaaccaatg ttcagcaagc aactcttcaa aaatttcgtt tgaaatgtct
    22081 cgtcgtaatt ttccatcaaa tgttaccgaa atatcaccag tttcttcaga tacaataacg
    22141 gtaaatgcat cagatacttc tgaaatacca accgcagctc tatgtcttgt acccaaactt
    22201 ttagatatct taggactatc agacaatggc aaataacttg ctgctgctgc aatcttcgtg
    22261 ccttgaataa tcattgcacc atcatgtaaa ggtgtgttag gtataaagac attaattaaa
    22321 agttcttgcg aaatatttga atccattgca atacctgttt caatataatc ttgaagacct
    22381 gtttcttttt caaagacaat taatgcacct atacgtcttt tagccatata ttgcacagcc
    22441 tttgaaaccg attgaatcaa tttctcttca tctttactat acgtattaga agtatagcgt
    22501 tttaaaaagc tacctctacc aagttgttct aacgcacgtc taatttctgg ttggaatatt
    22561 actattaaag ctaatacccc ccattgaata acgatatcga ataatttaga tgttgcagtc
    22621 aagttcaata tcatacttat ctgctgacca ataacaatta ctaatatccc tttaagtaat
    22681 tgtatcgctt tagttccctt aaagaccgtg atgagaagat aaagtacata ccaaactatc
    22741 agtaaatcaa ggatactcgt tacaattttt aacgtactga ggttttgaaa aaagttggaa
    22801 aaatccataa catctcctcc gggtaatatt tttccataat acccattata ccaatcattt
    22861 acatcactgt catacgaaca tatatttaaa tagaaaaaat ttattttaaa gattataact
    22921 actcttaatc attttagtga attaaaaaaa gtagtgcaaa aagcaaaata tactttatac
    22981 actacaaatc atttatttat aataaagttt caccaaaaaa tgttccaact aatgaaaccg
    23041 cttgttcagc agtatgatta ttactgtcaa tcaatggatt tacttcaact aaatccattg
    23101 aggaaattaa atgtgattga tgcagtaatt ccaatgcaaa atggctttct ctataactaa
    23161 gaccacccaa aactctagta ccagtgcctg gcgtttcaag cggatctaaa gcatcaacat
    23221 ctaaagataa atgaacgcca tcaacattgc gtgacttcaa atattctatt gtattttcaa
    23281 ttacttcctt tatccccaat ttatcaatat ctgacatagt aaatgtttta atattatgat
    23341 ctttgataaa ttgtctttca cctttatcta aatctctcat accaattagt acgatgtttt
    23401 ctggcttgat tacattacta tttaattcta aaagttcttt gggaccttcg cctgtcaaaa
    23461 tccttagagg cataccatga atatttccac ttggtgactc ttcaggtata tttaaatcac
    23521 catgtgcatc ataccaaata acacctaaat tattataatg tttacttatt gctgatactg
    23581 aacctaccgc aatagaatga tctccaccaa gaactagagg aaatctgtta ttttcaattg
    23641 atgctgaaac ctctttattt aatttttgat taacatctat aatttcatca taatttctta
    23701 atcctttttg ttcactatga aatttttcaa tgttcacaac aggtacctta atatccccct
    23761 tgtcatatac atcaaggtct aattgcttta atcttgaaat taatccagca tatctaattg
    23821 ctgttggtcc taaatcaaca cctaattttc tttgtccaaa tgttgatggt gcacctataa
    23881 tatcaattgc ttttgtcttt gtcataagcg tccccctttg ctctatgtaa ttaaagaata
    23941 atgtatgcgc ttaccattat caagcaatag ctacacatat aatctgttta tcttattact
    24001 tcataaaaaa aagcacttcc caaaaatgga aagtgcaagt agtgagccat agaggattcg
    24061 aacctctgac cctctgatta aaagtcagat gctctaccaa ctgagctaat ggctctaatg
    24121 gctgggctag ctggattcga accaacgagt gacggagtca aagtccgttg ccttaccgct
    24181 tggctatagc ccaatatata gatggtggag gggggcagat tcgaactgcc gaacccgaag
    24241 gagcggattt acagtccgcc gcgtttagcc acttcgctac ccctccagct tattcatata
    24301 atttaataat caaaatggtg gagaatgacg ggttcgaacc gccgaccctc tgcttgtaag
    24361 gcagatgctc tcccagctga gctaattctc caaaataatg actcctacgg gactcgaacc
    24421 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaaggagcc atggctccac
    24481 aggtaggact cgaacctacg accgatcggt taacagccga tagctctacc actgagctac
    24541 tgtggattaa tatt
//
LOCUS       DBNENW010000024        24262 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNENW010000024 DBNENW010000000
VERSION     DBNENW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.024"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            130..330
                     /locus_tag="AC1LW8_000774"
     CDS             130..330
                     /locus_tag="AC1LW8_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HIP4772631.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYINDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            353..823
                     /locus_tag="AC1LW8_000775"
     CDS             353..823
                     /locus_tag="AC1LW8_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772632.1"
                     /translation="MYNRKEIREMIDNYKWMKNIIDSKVYDNESTSIAQYGYQSAMPK
                     AKGTTSNKVLVKVINKNKALRKYDYLINKIAFIDEYEEYITNEKDYHILQMLKQRESH
                     NRIMSILDIGRDNFYSRVKDIVNILYNLQQETDTSYTSDSSDTSYKSYKSYTSD"
     gene            938..1390
                     /locus_tag="AC1LW8_000776"
     CDS             938..1390
                     /locus_tag="AC1LW8_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HIP4772633.1"
                     /translation="MEISKYQEIATRTHNDELNLNESITCYGLGLTQSTGNVTDLIKQ
                     HMFCNVPIDKGIMINELSEALWNIANLTNVLGINLDEIAGHSVNTILMNKPNQTINLD
                     NGIKQGDKVLFQGSKYLVDGSIGNLLLISNDKDDRQVTVQDVKKVDKE"
     gene            1406..1750
                     /locus_tag="AC1LW8_000777"
     CDS             1406..1750
                     /locus_tag="AC1LW8_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIP4772634.1"
                     /translation="MKRCGHPTCNVLINHNESYCDKHKQYANENYNDLRRRNDPEYLR
                     FYKSKTWQNMRRIVLLEHDFICVSCGNQATMVDHIVPTKIDWARRLDKSNLQPLCDAC
                     HNQKTKEDLKKY"
     gene            1879..2346
                     /locus_tag="AC1LW8_000778"
     CDS             1879..2346
                     /locus_tag="AC1LW8_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HIP4772635.1"
                     /translation="MAGRPKKLLSNSNKNYTKEEIIEKERQEAQLNKFSKIDTEPPHF
                     LDEIAKQEYLRILPHMQELPISNLDKAQLAQYCSFYSDFVKASLILEREDLILEDDKG
                     NQKVNPAFNIKEKAGIRLQQTANTLGLTIDSRLRIMVPDEKEDDDPYMEFVSD"
     gene            2349..4043
                     /locus_tag="AC1LW8_000779"
     CDS             2349..4043
                     /locus_tag="AC1LW8_000779"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIP4772636.1"
                     /translation="MTDYVTKYAKKVVSGEILASLKNIQVCKRHLSFMENPPNGCHWD
                     NHLSNKAIKFVEMLPDPKTNQPMPLMEFQKFIVGSLYGWRRGQYRMFTKAYISMARKQ
                     GKSLIVSGMSVNELLFGQYPKFNRQIYVASSTYKQAQTIFKMASQQVNLMRSKSKFIR
                     EKTDVRKTDIEDVLSSSVFAPLSNNPDAVDGKDPTVAILDELASMPDDEMYSRFKTGM
                     TLQKNPLTLLVSTAGDNLNSQMYQEYKYIKRILNEEVRADNYFVYCAEMDSQEEVQDE
                     TKWIKAMPLLESKEHRKTILQNVKADIQDELEKGTSYHKILIKNFNLWQAQREDSLLD
                     ISDWEQVITPMPNINGKDVYIGVDLSRLDDLTSVGFIFPNDDKKVFLHSHSFIGLRTN
                     LEQKSKRDKINYELAIERGEAETTQSDSGMIDYKQVIDFIVKFITTHDLNVQAVCYDP
                     WNAQSFITTIESMALDWPLIEVGQSFKALSQSIKEFRMWVADERIQHNDNMLLTTSVN
                     NAVLIRDGEDNVKINKKMNRQKIDPIISIITAFTEARMHEFQENWTEKYESEEFGF"
     gene            4057..4257
                     /locus_tag="AC1LW8_000780"
     CDS             4057..4257
                     /locus_tag="AC1LW8_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772637.1"
                     /translation="MDLNKINVFFNFLVANLVSILFLLGLFVVNVSMYKAFGQNIGLL
                     CIGITLIVISLILNHESNQERS"
     gene            4263..5513
                     /locus_tag="AC1LW8_000781"
     CDS             4263..5513
                     /locus_tag="AC1LW8_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIP4772638.1"
                     /translation="MGIFYKNEKRDLQYNEDDLQMMVQTLPGFQGTKLRQYKDIEAIR
                     HSDIFTAVMMIASDLARMPIRVTVNGQINYSDRIVNLLNTRPNPMYNGYIFKLVVFVS
                     ALLTSHGYIEITRDKTGEPMNLTFRKTSEIELKSDARGRLYYFHQRIDSNGNNIERNV
                     KFEDMLDIKFYSLDGINGLSLLDTLSRTIESDNNGKDFLNNFLRNGTHAGGILKMKGV
                     LDNKKARDRAREEFHKSFSGTKQAGKVVVLDESMTFDQLEVDTEVLKLIRENKSSTRE
                     IAGVFGIPLHKFGIETANMSITDANLDYLSTLKPYITCVCAELNFKFNDEYVNREFKF
                     DTTEIRVVDEKTQAEIDKINIDSGKMNIDEIRQRDGLAPIPGGNGSIHRVDLNHVNIE
                     LVDEYQMNKSRATDKKLKGGEENE"
     gene            5506..6090
                     /locus_tag="AC1LW8_000782"
     CDS             5506..6090
                     /locus_tag="AC1LW8_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HIP4772639.1"
                     /translation="MSKETRVGNIIEVRSNDNNEMVIEGYALKFDTWSENLGGFKETI
                     SRRALENTDLSDVRCLVDHIPSQIIGRTKSGTLELETDDVGLKYRCKLPNTTFARDLY
                     ENMRVGNINQCSFGFMLDDKGDEVRFDEQENIYKRTLTAIRELTDVSVVTYPAYKDTD
                     VKPALRSIETVKKEQRKKELEIRLKKHSILNNIW"
     gene            6178..7425
                     /locus_tag="AC1LW8_000783"
     CDS             6178..7425
                     /locus_tag="AC1LW8_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIP4772640.1"
                     /translation="MKTKEELQSEISDIKRQIDLKVKYATRALNNDELEKAEKLEQEI
                     TDLRSQIQEKQEELDKLKEKDGTSENNQQSVEVNEARTYRNQANINDLGISIQNTKVT
                     SQEVRDFTEYLETRNDIQGGSLKTDSGFVVIPEEIVTDILKLKEVEFNLDKYVTVKRV
                     TNGSGKYPVVRQSEVAALEKVEELEENPELAVKPFFQLAYDINTHRGYFRISREAIED
                     AKVNVLQELKLWMARTIAATRNKAIIDVITKGSTGSTSSGFEKEGKKLEVKKAKSLDD
                     IKDAINLNVKPNYEHNVAIVSQTMFAKLDKMKDKLGNYLIQPDVKEKTQQRLLGAKIE
                     ILPDEVLGQKGNNTLIIGNLKDAIVLFDRSQYQASWTDYMHFGECLMIAVRQDCRILD
                     YKSAIVIEYDDSERGEGDLGLEA"
     gene            7461..7619
                     /locus_tag="AC1LW8_000784"
     CDS             7461..7619
                     /locus_tag="AC1LW8_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772641.1"
                     /translation="MAMYEVKKSYTDLEKGQYLKSGKRVEMTVKRAEYVNKKLKEHGV
                     ILERVKEE"
     gene            7628..7960
                     /locus_tag="AC1LW8_000785"
     CDS             7628..7960
                     /locus_tag="AC1LW8_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIP4772642.1"
                     /translation="MQLTAEELKLLKKHCKIDHNSEDDLLEIYYSWAFHEIASAVTDE
                     PSKYIDWFKSHPLFARAIYPLASYYFENRIAYLDRDLSLAPHMVLSTVHKLRGSFEQF
                     LESENDEI"
     gene            7947..8282
                     /locus_tag="AC1LW8_000786"
     CDS             7947..8282
                     /locus_tag="AC1LW8_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head completion protein"
                     /protein_id="HIP4772643.1"
                     /translation="MMKFNSNKLNERIDFCEDVSERVNGNPMKPKTKILYSCFACIQE
                     SKESDTQTNLNTGSKFIKTIIIRDTRGDYKPTNKHYVLHEGQRFNIKYVKPDYQDKSY
                     LRIYGEVVI"
     gene            8282..8659
                     /locus_tag="AC1LW8_000787"
     CDS             8282..8659
                     /locus_tag="AC1LW8_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HIP4772644.1"
                     /translation="MGARIESNNIEQGLKNAVLKMNLNSNVIVKAGAMSLVPLLKSNT
                     PFANTKKHARDHIAVSNVKTDRHTSEKIVTIGYAKGVSHRIHATEFGTMYQKPQLFIT
                     KTEKQGKNKVLKTMLDTAKRLQK"
     gene            8656..9036
                     /locus_tag="AC1LW8_000788"
     CDS             8656..9036
                     /locus_tag="AC1LW8_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772645.1"
                     /translation="MINVTKLIRNAIIANNITDEVNVFNYTIDDHFHEKTDKPIIRIY
                     PLPFNPDTYADDNEISREYHYQIDVWWSQDEPNEQVEKIVELLKVINFQCYYREPLYE
                     SDVMSFRHIIRAKGSILSMKLEEN"
     gene            9037..9990
                     /locus_tag="AC1LW8_000789"
     CDS             9037..9990
                     /locus_tag="AC1LW8_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HIP4772646.1"
                     /translation="MIEKLKQAPRFLKLNLQHFADTGVSGIAIGVSNFYYAPILKDTE
                     NEWETGAGTRIRFLKEIEVDRPQDTEEDYGDDMVAATAVSNGKLSVKTTFVTVPADDK
                     AFLNGAKKGVGGYKYGAKDIPPDVAIVFERRNHDESSEWVGLFKGKFTRSSIKGQTKQ
                     DKVEFQNDDVEGNFIDRLFDESSHVTGYDKKGSTTGRDYVFMETFGKTYDEFMSSRGE
                     QNMEPVEKEMKKTEKVEVTSVNVTDEQVTVKVDATKQLSATTEPSGQKVTYAVTEGQT
                     YASVTSTGLVKGLAEGNATVTATAGKQTDTVQITVQSNLEM"
     gene            10055..10501
                     /gene="gpG"
                     /locus_tag="AC1LW8_000790"
     CDS             10055..10501
                     /gene="gpG"
                     /locus_tag="AC1LW8_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone G"
                     /protein_id="HIP4772647.1"
                     /translation="MERTSIELITGFTKTGKPQYQKYLAKPIITLFETIQGSKLGLKL
                     NKAFKGADFKDLTEEEFNNLSVTEQEEYKNKQEEYENNMAVQMEVLEEVLDFIVEAFD
                     NQFTSIELQKGLPNGQEGIEKIGQLIGRITGGEPSDTKKFVTENQK"
     gene            10561..10683
                     /gene="gpGT"
                     /locus_tag="AC1LW8_000791"
     CDS             10561..10683
                     /gene="gpGT"
                     /locus_tag="AC1LW8_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone GT"
                     /protein_id="HIP4772648.1"
                     /translation="MIEKGMDAEKVANMPIHFFLDIVESKIETKRTAKSFKDIF"
     gene            10739..15388
                     /locus_tag="AC1LW8_000792"
     CDS             10739..15388
                     /locus_tag="AC1LW8_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIP4772649.1"
                     /translation="MPNPIGNMVIKVDLDGSGFNRGVTGLNRQMKMVSRELSANLSQF
                     SRYDNSLEKSKIKVEGLSKKQKVQAQITKELKDSYDKLSKETGENSAKTQAAAAKYNE
                     AYAKLNQYERELNQATQELKDMQREQKALNTAMGKLGTNFNNFGPKLQEIGNSMKNVG
                     RNMTMYVTAPVVAGFAVAAKKGIEFDDSMRKVKATSGATGEEFEALKKKAREMGATTK
                     FSASDSAEALNYMALAGWDSKQMMEGLSGVMDLAAASGEELGAVSDIVTDGLTAFGLK
                     AKDSGHFADVLAQTSSKANTDVRGLGEAFKYVAPVAGALGYTIEDTSIAIGLMSNAGI
                     KGEKAGTALRTMFTNLSSPTRAMGNEMERLGISITDSNGKMIPMRKLLDQLREKFKHL
                     SKDQQASSAATIFGKEAMSGALAIINASDEDYQKLTKSIDSSTGASKRMADTMESGLG
                     GKLRTLRSQLEELALTIYDRIEPALKIIVSAFSKVVTWVTKLPTSIQLAVVEFGLFVA
                     VLGPLVFMFGLFISVMGNAMTVLGPLLINVNKASGLFAFLRTKIASLVKLFPILGVSI
                     SSLTLPITLIVGALVGIGIAFYQAYKRSETFRNIVNLAISGVANAFKAAKLALQGFFD
                     LFKGDSKGAVTLEKIFPPETVAGIQNVVNTIRTTFFKVVDAIVGFAKEIGAQLASFWK
                     ENGSEITQALQNIAGFIKATFEFIFNFIIKPIMFAIWQVMQFIWPAVKALIVSTWENI
                     KGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLIQLWFVGKILG
                     VVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKSIFTNMKNWLS
                     STWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWNSIKDNTVGIA
                     GRLWDKVRNIFGSMRDGLKSIIGKIKDHIGGMVDAVKRGLNKLIEGLNWVGGKLGMDK
                     IPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMIEFPNGKRVLT
                     PNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSIGTMWKDIKSGASSAFNWTKDQIGK
                     GTKWLGDKVGDVMDFIDNPGKLLNYVLQAFGVDFSSLTKGMGIAGDITKAAWSKIKKS
                     AIKWLEDAFAESGDGGVLDMNKLRYLYGHTAAYTRETGRPFHEGLDFDYIYEPVPSTI
                     NGRAQVMPFHNGGYGKWVKIVKGALEVIYAHLSKYKVKTGQQVRVGQTVGISGNTGFS
                     TGPHLHYEMRWNGRHRDPLPWLRKNNGGGKSTPGGNGAANARRAIKAAQNILGGRYKA
                     SWITNEMMRVASRESNYTANAVNNWDSNARAGIPSRGMFQMIDPSFRAYAKSGYNNPL
                     NPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYATGGKVYNGLYHLGEEGYPEWI
                     IPTDPSRANEAHKLLALAANDIDNRSKNKRPNNLPNPSISNSDTNYIHTLENKLDAVI
                     NCLVSLVESNQVIADKDYEPVINKYVFEDEVNNSIDKRERHESTRVRFRRGGTII"
     gene            15388..16878
                     /locus_tag="AC1LW8_000793"
     CDS             15388..16878
                     /locus_tag="AC1LW8_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein"
                     /protein_id="HIP4772650.1"
                     /translation="MQDTIQIDNKTIEWLVVQRGFEIPSFNFVTEKESVKGRTGSIAK
                     ARYLNDIEFELPLIIRNEVLAPGGQKTHDDILEELVEFFDIDNLKPKKLKFKSQNWYW
                     FAYFDGPLKLPKNPRGSVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPL
                     IVEARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITE
                     DIPSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCI
                     VEQKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQ
                     NKPIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQ
                     RPASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSV
                     VINEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            16894..20676
                     /locus_tag="AC1LW8_000794"
     CDS             16894..20676
                     /locus_tag="AC1LW8_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIP4772651.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKITSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLDKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTQALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRKTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDYNRIIIDSYASANIESREAPIYLSPNTKNKPGLNRFAFTLSNADSAYET
                     DGYIMFGSDENYKYGAGLRFSKRSNKGLVQVVNGDYATGGDTTIESGMGKFNLVKRRD
                     GNSYVSIQSYDLLAVGSDNAGDRVASNSIYKRTYSAPANLHITSAGTIGRATSAKKYK
                     ISIENQYINEDDQFSHSKEILKLPIRTWFDKYESEIMAKELESGKKLSDDTFKLSRHT
                     GLIAEEVEELGFNEFVIYDDNGEIEGIAYDRLWVHLIPIIKNQQSKIEKLEELINE"
     gene            20669..20821
                     /locus_tag="AC1LW8_000795"
     CDS             20669..20821
                     /locus_tag="AC1LW8_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772652.1"
                     /translation="MNDSNQGLQANPQYTIHYLSQEITRLTQENAMLKAYIQEQNEKS
                     KSAEEE"
     gene            20867..21154
                     /locus_tag="AC1LW8_000796"
     CDS             20867..21154
                     /locus_tag="AC1LW8_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772653.1"
                     /translation="MANEIIKKTERFILVQIDKEGTERVLYQDFVGSFTTSDSASYAQ
                     DFKSEENAKKIAETLNLLYQLTGNQNGVKVVKEVVDRTDLSSDKSVDSETM"
     gene            21210..21584
                     /locus_tag="AC1LW8_000797"
     CDS             21210..21584
                     /locus_tag="AC1LW8_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772654.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            21957..22730
                     /gene="sea"
                     /locus_tag="AC1LW8_000798"
     CDS             21957..22730
                     /gene="sea"
                     /locus_tag="AC1LW8_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type A"
                     /protein_id="HIP4772655.1"
                     /translation="MKKTAFTLLLFIALTLTTSPLVNGSEKSEEINEKDLRKKSELQG
                     TALGNLKQIYYYNEKAKTENKESHDQFLQHTILFKGFFTDHSWYNDLLVDFDSKDIVD
                     KYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWLDGKQN
                     TVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVFHTSTEPSV
                     NYDLFGAQGQYSNTLLRIYRDNKTINSENMHIDIYLYTS"
     gene            22881..23015
                     /gene="pepG1"
                     /locus_tag="AC1LW8_000799"
     CDS             22881..23015
                     /gene="pepG1"
                     /locus_tag="AC1LW8_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin PepG1"
                     /protein_id="HIP4772656.1"
                     /translation="MVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK
                     "
     gene            complement(23068..23175)
                     /locus_tag="AC1LW8_000800"
     CDS             complement(23068..23175)
                     /locus_tag="AC1LW8_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772657.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPSEPVKKTVAILD"
     gene            23223..23477
                     /locus_tag="AC1LW8_000801"
     CDS             23223..23477
                     /locus_tag="AC1LW8_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIP4772658.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            23489..24244
                     /locus_tag="AC1LW8_000802"
     CDS             23489..24244
                     /locus_tag="AC1LW8_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4772659.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT         8959 a         3395 c         4947 g         6961 t
ORIGIN      
        1 acgaaaaata aacgattata atctttatct tgataaattt gaagaacagt ttagtcaaaa
       61 gtttaaaata aacagaactt cgataaaaga aagaattatt attaatttaa acaagaggag
      121 atttaaatga tgtggattac tatgactatt gtatttgcta tattgctatt agtttgtatc
      181 agtattaata gtgatcgtgc aagggagata caagcgctca gatatataaa tgattatcta
      241 cttgatgaag tagttaaaac taaaggatac aacgggttaa aagaatacag gattgaatta
      301 aagcgaatga ataacgatat taaaaagtaa tttatattat cggaggtatt gcatgtataa
      361 caggaaagaa atacgtgaaa tgatagataa ctacaagtgg atgaaaaata taatagacag
      421 taaagtctac gataacgaaa gtacatcaat tgcacaatac ggttatcaat ctgcgatgcc
      481 aaaagctaaa ggcacgacta gcaataaagt gttagtgaaa gttataaaca aaaacaaagc
      541 gcttagaaag tacgattact tgattaataa gatagcgttc attgatgaat acgaagaata
      601 catcacgaat gaaaaagatt atcatatttt acaaatgtta aaacaacgag aaagccataa
      661 taggattatg agtattcttg atataggcag agacaatttt tattctagag taaaagatat
      721 agtaaatata ctttataact tgcaacaaga aaccgacaca tcgtacacat cggacagttc
      781 ggacacatcg tacaaatcgt acaaatcgta cacatcggac taattttgat gctacatatt
      841 gttttttatt ataattgcta tgtagcaaaa catttatatt tattttgaac tctcacatta
      901 agtgagggtt tttattttta taaacaagag gtggagaatg gagatatcaa agtaccaaga
      961 gatagctaca cgtacacaca atgatgaatt gaatttaaat gaatctatta cttgttacgg
     1021 cttaggttta actcaatcta caggcaatgt tacagatcta attaaacagc atatgttttg
     1081 taatgtaccg atagataaag gaattatgat aaatgaactt agcgaagcat tgtggaatat
     1141 agctaatctt actaacgtgt taggtattaa cttggatgag atagctggtc atagtgttaa
     1201 cactatcttg atgaataaac ctaatcagac tatcaattta gacaatggta taaaacaagg
     1261 agacaaagta ttgtttcaag gtagtaaata tcttgttgat ggatcgatag gaaacttatt
     1321 gttaattagc aatgataaag atgatagaca agtaactgtg caagatgtta agaaagtcga
     1381 caaggagtga tgtgcattgt ctattatgaa gcgatgtggt catccaacat gtaatgtatt
     1441 gattaatcat aatgaaagtt attgtgataa acacaagcaa tatgcaaatg aaaattacaa
     1501 tgatttgaga cgtcgaaacg atccagagta tttaagattt tataaatcga aaacgtggca
     1561 aaacatgcgt cgaattgtat tgttagaaca tgattttatt tgtgtttctt gtggcaatca
     1621 agcgactatg gttgaccata ttgtaccaac aaaaattgat tgggcaagaa gattagacaa
     1681 aagtaattta cagcctttgt gtgatgcttg ccataaccaa aagacaaaag aagatttgaa
     1741 gaaatattaa aaaagataaa aataggaagt cccccaaaga tgaaacgggc gtcaatgaaa
     1801 ggttctggag aacggagcag agttttcttc tcaaaaaatt ccctttattt aagttttttt
     1861 agtaggaggt gctaatttat ggcgggtaga cctaagaagc ttttgtcaaa ttcgaacaag
     1921 aattatacaa aagaagaaat tattgaaaaa gagcgtcaag aagctcaatt aaataaattt
     1981 tctaaaatcg atactgaacc accgcacttt ttagatgaaa tagcgaaaca agaatactta
     2041 agaatattac cgcacatgca agaattgcca atttccaact tagataaagc acaattagca
     2101 caatattgta gtttttatag tgactttgtt aaagcaagtt tgattttaga gcgcgaagac
     2161 ttgattttag aagacgacaa aggaaatcaa aaggttaatc cggcgttcaa cataaaggaa
     2221 aaagcgggta ttcgattgca acaaacagct aatactttag gattaactat tgatagccga
     2281 ttgcgtatta tggttcctga tgaaaaagaa gatgatgatc catatatgga atttgtgagt
     2341 gattagtaat gactgattat gttactaaat acgcaaaaaa ggtagtttca ggagaaattt
     2401 tggcaagttt gaagaatatt caagtatgca aacgtcacct atcttttatg gagaacccgc
     2461 cgaatggttg ccattgggat aatcatttgt ctaacaaagc aattaaattt gtggaaatgc
     2521 ttccagaccc taaaacaaac cagcccatgc ctcttatgga gtttcagaaa ttcattgttg
     2581 ggagcttata cggctggcgt agaggtcaat acagaatgtt tactaaagct tatataagta
     2641 tggctagaaa gcaaggtaag tctctaatcg tatcgggaat gtccgttaac gaactgttgt
     2701 ttggacaata ccctaaattt aatagacaaa tttatgtagc ttcatctact tataagcaag
     2761 cgcaaacaat attcaagatg gcaagccaac aagtaaacct aatgcgaagt aaaagcaagt
     2821 ttatccgtga aaaaacagac gtaagaaaga cagacattga agatgtatta agtagttcag
     2881 tgtttgcacc tctttccaat aacccagatg cggttgatgg taaagatcct acagttgcta
     2941 ttttggacga attggcaagt atgcctgatg atgagatgta ctcaaggttt aaaacaggta
     3001 tgacattaca aaaaaatcct ttaaccctac ttgtttcaac ggccggagac aatttaaata
     3061 gtcaaatgta ccaagagtat aagtatatta aacgtatttt aaatgaagaa gtaagagctg
     3121 ataattactt tgtatattgt gctgaaatgg attcacaaga agaagttcaa gatgaaacaa
     3181 agtggattaa agcaatgccg cttttagaat caaaagaaca tagaaaaact atacttcaaa
     3241 atgtaaaagc tgatatacaa gacgaattag aaaaagggac atcgtatcat aagattttga
     3301 ttaaaaactt caatttatgg caagcgcaaa gagaagatag cttgctagat atttcagatt
     3361 gggaacaagt aataacgcct atgcctaata tcaatggtaa agatgtgtat ataggtgtcg
     3421 acttatcgag attggatgac ttaacatctg tagggtttat tttccctaac gacgataaaa
     3481 aagtgttttt acatagtcat tctttcattg gattaagaac aaacttagaa caaaaatcta
     3541 agagagacaa aataaattat gaattagcga ttgaacgtgg agaagctgag actacacaat
     3601 cagatagcgg catgattgat tataaacaag ttatcgattt tatagtgaaa tttataacga
     3661 cgcatgacct gaatgtacag gctgtttgct atgacccttg gaatgcgcaa agttttataa
     3721 caacaatcga atcaatggct ttagattggc cactcattga agtgggacaa agttttaagg
     3781 cgttatcaca atctattaaa gaatttagaa tgtgggttgc agatgaaaga atacagcata
     3841 acgataatat gttacttaca acatcagtta ataatgccgt tttgattcgt gacggagaag
     3901 acaatgtgaa aataaataaa aaaatgaatc gtcaaaaaat agatccgatt atttcgatta
     3961 tcacagcttt cactgaagct agaatgcacg aattccaaga aaattggacg gagaaatatg
     4021 aaagcgaaga attcggattt taaaggtggt gacaaaatgg acttgaataa aataaatgtc
     4081 ttttttaatt tcttggttgc taatttggtt agcatccttt ttttattagg tttgtttgtg
     4141 gttaatgttt ctatgtataa agcattcggt caaaatatag gacttttatg cattggtata
     4201 acactgattg ttatttcgtt gattttaaat catgaaagca atcaagaaag gagttagtag
     4261 ttgtggggat tttttataaa aatgaaaaac gagacttgca atacaacgaa gatgatttgc
     4321 aaatgatggt tcaaactttg ccaggttttc aaggaacaaa attacgacaa tataaagata
     4381 tagaagcaat taggcatagc gacatcttta ctgcagttat gatgattgct tctgatttgg
     4441 cgcgcatgcc aattagggtg acagtgaacg gccaaattaa ttatagtgac aggattgtta
     4501 atttgttaaa tacacgtcct aacccaatgt ataacggcta tatattcaaa ttagtagtgt
     4561 ttgttagtgc cttactaaca tcgcacggct atattgaaat tacacgtgat aaaacaggag
     4621 aacctatgaa tttaacgttc agaaagacat ccgaaataga attgaaatca gacgcaagag
     4681 gtcgactgta ttattttcat caaaggatag acagtaacgg aaataatata gaacgtaatg
     4741 ttaagtttga ggatatgcta gacatcaaat tttattcgtt ggatggtata aatggtttgt
     4801 cactgttaga cacattaagt cgcacgatag aatcagataa caatggaaaa gatttcctta
     4861 ataatttctt gcgaaatggc acacatgctg gtggtatttt gaaaatgaaa ggtgtattag
     4921 ataataaaaa agcaagagac cgtgccagag aagaatttca caaaagtttt agtggaacta
     4981 aacaagctgg gaaagttgtc gtactcgatg aatcaatgac gtttgatcaa ttagaagttg
     5041 atacagaagt tttaaagctt atcagagaaa acaaatcatc aacaagagaa atagcaggtg
     5101 tatttggtat tccattgcat aagttcggca tagaaacagc gaacatgagt atcacggatg
     5161 ctaatttaga ttacttatca actttaaaac cttatattac atgcgtttgt gcagaattga
     5221 attttaagtt taatgatgaa tatgtgaatc gtgaatttaa atttgatacc actgaaatac
     5281 gagttgttga tgaaaaaaca caagctgaaa ttgacaaaat taacattgat tctggaaaga
     5341 tgaatatcga tgaaattaga caacgtgatg gattagcgcc aataccaggc ggtaatggta
     5401 gcattcacag agtcgattta aaccatgtaa atattgaact tgtagatgag tatcagatga
     5461 ataaatcgag agctactgat aaaaaattga aaggtggtga ggaaaatgag taaggaaacg
     5521 agagttggca acattattga ggtacgctca aatgataaca acgaaatggt catagagggg
     5581 tatgcgttaa agtttgacac ttggtctgaa aatcttggtg gattcaaaga aacgatttca
     5641 cgtcgcgctt tagaaaacac tgatttatct gatgtacgtt gtttagtaga tcatatccca
     5701 tcgcaaataa ttggtaggac aaaatcgggt actttggagc tcgaaactga tgatgttgga
     5761 cttaaatatc gttgtaagtt accaaacaca acatttgcac gtgatttata tgagaacatg
     5821 cgtgtaggca acatcaatca atgttcgttt ggttttatgc ttgacgataa aggcgatgaa
     5881 gtgcgttttg atgaacaaga aaacatttac aaacgtactt taacagcaat tcgtgaactt
     5941 acagatgttt ctgtagtgac ttatccggct tacaaagaca ctgatgttaa accagcatta
     6001 cgtagtattg aaaccgttaa aaaagaacaa cgtaaaaaag aattagaaat aagactaaag
     6061 aaacactcta tattaaataa tatttggtga agttgaacac cattatcaaa tacagccatt
     6121 ggacatgctg aatatagcga tgtctatttt tttatgccaa ttttaggagg aaattaaatg
     6181 aaaacaaaag aagagttaca atctgagatt tcagacatta aaagacaaat tgatttaaag
     6241 gtgaagtatg caacgagagc acttaataac gatgagttag aaaaagcaga aaaattagaa
     6301 caagaaatta ctgatttacg ttctcaaatc caagaaaaac aagaagaatt agataagcta
     6361 aaagaaaaag atggaacttc agaaaacaat caacaatcag tggaagtaaa cgaagcacgt
     6421 acttatcgaa accaagcaaa cattaatgat ttaggtattt cgattcaaaa cacaaaggta
     6481 acatcacaag aagttagaga ttttactgaa tatcttgaaa cacgcaatga tattcaaggt
     6541 ggttcgttaa aaacagactc aggatttgta gttattccag aggaaattgt tacagatatt
     6601 ttaaaattaa aagaggttga gtttaatctt gataagtatg tgacggtcaa acgtgttaca
     6661 aatggttctg gtaaatatcc ggtagtacga caatcagaag ttgcagccct tgaaaaagtt
     6721 gaagaattag aagaaaaccc tgaattagca gttaaaccat tcttccaatt agcatatgac
     6781 attaatacac accgtggtta cttccgaatt tcacgtgaag caatcgaaga tgcaaaagtg
     6841 aatgttttgc aagaattgaa actatggatg gcgcgaacta ttgcagcaac acgaaacaaa
     6901 gcaattattg atgttatcac taaaggatca acgggttcta caagttcagg ttttgaaaaa
     6961 gaaggtaaga aattagaagt taaaaaagca aaatctttag atgatattaa agatgctatt
     7021 aacctgaatg ttaagccaaa ttacgaacat aatgttgcga ttgtttcgca aactatgttt
     7081 gcaaaattag acaaaatgaa agataagcta ggaaactatt taatccagcc agatgttaaa
     7141 gaaaaaacgc aacagcgttt attaggagct aaaatcgaaa ttttacctga tgaagtacta
     7201 gggcaaaaag gtaataacac tttgattatc ggtaacttaa aagatgcgat tgttttattt
     7261 gaccgctctc aataccaagc atcatggact gactacatgc atttcggaga atgtttaatg
     7321 attgctgtac gtcaagactg tagaattcta gattataaat cagcaattgt gattgaatat
     7381 gatgatagtg aacgcggtga aggcgatctt ggcttagaag cataataagc gctcgatact
     7441 ttataaagag gtgataaact atggcaatgt atgaagtgaa gaaatcttat actgacttgg
     7501 aaaaaggcca gtatttaaag tcaggtaaac gtgttgaaat gacagtaaaa cgtgctgaat
     7561 atgttaacaa aaagctgaaa gagcatggag taatacttga aagagtaaaa gaagaatagg
     7621 tgattgaatg caattaacag ctgaggaact taagttatta aaaaagcatt gcaaaataga
     7681 tcacaattca gaggacgact tattagaaat atattactct tgggcattcc atgaaatagc
     7741 tagcgctgtt acggatgaac caagtaaata tattgattgg tttaaaagtc atcctctatt
     7801 tgctcgtgct atataccctt tagcaagtta ctattttgaa aaccgtattg cttatttgga
     7861 tagggattta tcgcttgcgc cacatatggt tttaagtacg gtgcataaat tgagaggttc
     7921 atttgagcaa tttttggaga gtgaaaatga tgaaatttaa ttccaataaa ttaaatgaac
     7981 gtatagattt ttgtgaagat gtaagcgaga gagtgaacgg aaatccgatg aaaccgaaga
     8041 cgaaaatatt atactcttgt ttcgcttgca ttcaagaatc taaagaatcc gacactcaaa
     8101 cgaatctcaa tacaggtagc aaattcatta aaactattat tatcagagat acacgaggtg
     8161 attataaacc aacaaataag cattacgtct tgcatgaagg gcaaagattt aacatcaaat
     8221 atgtaaagcc agattatcaa gataaatctt atttgcgtat ctatggcgag gtggtcattt
     8281 aatgggggca agaattgaaa gtaataacat cgaacaaggt ttgaaaaatg cagttttaaa
     8341 aatgaattta aatagtaatg taattgtcaa agctggggct atgtcattag tcccgctttt
     8401 aaaaagtaat acaccttttg cgaatactaa aaagcatgct cgcgatcaca tagctgtttc
     8461 taatgtgaaa acagacagac acacaagtga gaaaatcgtt acaattggtt acgctaaagg
     8521 cgtctcacat cgtattcatg caacagaatt tggaacaatg taccaaaaac cacaattgtt
     8581 tataacaaaa acagaaaagc aagggaaaaa caaagtttta aaaacaatgc ttgatactgc
     8641 taagaggttg caaaaatgat taatgttacc aaattaatta gaaacgctat tattgcaaat
     8701 aacattacag atgaagtgaa tgtgtttaac tacactatag atgaccattt tcacgaaaaa
     8761 actgacaagc ctattattcg catatatccc ttaccgttca atcctgacac atacgctgat
     8821 gataacgaga tttcaagaga ataccattac caaattgatg tttggtggtc tcaagatgaa
     8881 ccgaacgagc aagtagaaaa aattgttgag ttactcaaag tgataaattt tcaatgttat
     8941 tacagagaac cgttatacga gagtgacgtc atgtcattca gacatattat aagagcaaaa
     9001 ggctcgattt tatcaatgaa attggaggaa aattaaatga ttgaaaaatt gaaacaagca
     9061 ccaagatttt taaaattaaa cttacaacat tttgcagata caggagtttc gggtatcgca
     9121 attggggtat caaactttta ttatgcacct attttaaaag atacagaaaa tgaatgggaa
     9181 actggagctg gcacacgtat tcgtttctta aaagaaattg aagtagaccg tccacaagat
     9241 accgaggaag attatgggga tgatatggtc gcagcaactg ctgtatctaa tggtaaacta
     9301 agtgttaaga caacatttgt tactgttcct gctgacgata aggcgttctt gaatggcgct
     9361 aaaaaaggtg taggtggtta taaatatgga gctaaggata tcccgccaga tgtagcgatt
     9421 gtatttgaaa gacgtaatca tgatgagtct tcagaatggg ttggcttgtt caaaggtaaa
     9481 ttcactcgtt caagcatcaa agggcaaaca aaacaagata aagttgaatt ccagaatgac
     9541 gacgtagaag gcaattttat tgatcgtttg tttgatgaga gctcgcatgt tactggctat
     9601 gataaaaaag gaagcactac agggcgcgat tatgtattca tggaaacatt tggtaaaact
     9661 tatgatgaat tcatgtctag tcgaggagaa caaaatatgg aacctgtaga aaaagaaatg
     9721 aaaaaaacag aaaaagttga agtcacttct gtaaacgtca ctgatgaaca agttacagtt
     9781 aaagttgatg ctactaaaca actatcagcc acaaccgaac catctggaca gaaagtaact
     9841 tatgcagtga ctgaggggca aacgtatgct agcgtaacat caactggcct cgttaaaggt
     9901 ttggcggaag gtaatgcgac cgttacagcg actgcaggaa agcaaactga tactgtgcaa
     9961 attacagtac aatctaattt agaaatgtaa gttttgaggg cttaacgccc tctttttatt
    10021 ttggccaaat taaaaagaaa gtaggaattt aataatggaa cgtacatcaa ttgaattaat
    10081 tacaggattt acaaaaacag gaaagccgca atatcaaaag tatttagcga agccgattat
    10141 tactttgttt gaaacaattc aaggttcaaa attaggtttg aaacttaaca aagcctttaa
    10201 gggggctgat tttaaagatc taacagaaga agaatttaat aacttaagtg tgacagaaca
    10261 ggaagaatac aaaaacaagc aagaagaata cgaaaacaac atggctgtac aaatggaagt
    10321 attagaagaa gttttggatt tcatcgttga agcttttgat aatcaattta ccagtataga
    10381 acttcaaaaa ggattaccaa atggtcaaga aggtattgaa aagattggac agttaattgg
    10441 acgaattaca ggtggggaac ctagcgatac aaaaaagttc gtgacagaga atcagaaata
    10501 agaaaagaag atttaacacc tgaagctgtc tacaacaatt acaggaaaat agctaaagat
    10561 ttgatagaaa aaggcatgga tgcagaaaaa gtggctaaca tgccgataca cttcttttta
    10621 gacattgtcg aatcgaagat tgaaacaaag cgaactgcga aaagttttaa agatattttt
    10681 taatcagcct ttaaaggttg attttttatt tacatcttgg aagaaaggag gtttttaaat
    10741 gcctaatcct ataggtaata tggtcataaa ggttgattta gatggttctg gattcaatag
    10801 aggtgtgaca ggtttaaata ggcaaatgaa aatggtttcg cgtgagcttt cggctaattt
    10861 atcacaattt tctagatatg ataattcatt agaaaagtcg aagataaaag tcgaaggttt
    10921 gagtaaaaaa caaaaagttc aagcccagat tactaaagag ctgaaagata gttatgacaa
    10981 acttagtaaa gaaactggtg aaaacagtgc aaagacacaa gctgcggctg ctaaatacaa
    11041 tgaagcttac gctaaattaa accaatatga gcgagagtta aaccaagcca cacaagaatt
    11101 aaaagacatg caaagagagc agaaagcatt aaatactgca atgggaaaac ttggtaccaa
    11161 ctttaataat tttggtccta aacttcaaga aattggtaac agtatgaaaa atgtaggccg
    11221 taacatgact atgtatgtaa ctgcgccggt ggttgctggg tttgctgtag cagctaaaaa
    11281 aggtattgaa ttcgatgaca gtatgagaaa agttaaagca acttcaggtg ctactgggga
    11341 agagtttgaa gctttgaaga aaaaggctcg cgaaatgggt gcaacaacaa aatttagtgc
    11401 atcagattcg gctgaagcat taaattacat ggcacttgct ggttgggatt ctaagcaaat
    11461 gatggaaggt ttaagcggag ttatggattt agcggcagca tctggcgaag aactgggagc
    11521 agtaagtgac attgttacag atggactaac ggcatttggt ttaaaagcaa aggatagtgg
    11581 tcattttgcg gacgttttag cacaaactag ctcgaaggca aatacggatg ttagagggct
    11641 cggagaagct tttaaatatg tcgctcctgt agcaggtgcg ttaggttaca cgattgaaga
    11701 tacatctatt gcgataggtt taatgagtaa tgctggtatc aaaggtgaaa aagcaggtac
    11761 agcgttacga acaatgttca ccaatctttc aagtccaact agagctatgg ggaatgaaat
    11821 ggaacgctta ggaatatcta ttacagatag taatgggaaa atgattccta tgcgaaagct
    11881 tttagaccaa ctgagggaaa aatttaaaca tctttcaaaa gaccaacaag ctagttctgc
    11941 agctacaata tttggtaaag aagcgatgtc aggagcatta gcgattataa atgcttctga
    12001 tgaagactat caaaagttaa ccaaatctat agattcatct accggcgcat ctaaaagaat
    12061 ggccgataca atggaatctg gtttaggtgg gaaattaaga actttaaggt cgcaattaga
    12121 agaactagcc ctaacgattt atgacagaat agaaccagca ctaaagatta tagtaagtgc
    12181 ttttagcaaa gtagtgacat gggttactaa attaccaacg tcaattcaat tagcggttgt
    12241 tgagtttgga ttatttgtag cagttttagg tcctttagtt tttatgttcg gtttatttat
    12301 cagcgtgatg gggaatgcaa tgacagtttt aggacccttg ttaataaacg ttaataaagc
    12361 tagtggttta ttcgcgtttt taagaactaa aatcgcatca cttgttaaac tatttccgat
    12421 tttaggtgtg tcgatatcaa gtttaacgtt gcctataaca ttaattgtag gtgcattagt
    12481 tggtattggc atagctttct atcaagctta taaacgttca gaaactttta gaaatattgt
    12541 aaatctggca atctctggtg tagcaaacgc atttaaagca gctaaactag cgttacaagg
    12601 tttctttgat ttattcaaag gtgatagtaa aggcgcggtt accctagaga agatatttcc
    12661 acccgaaact gtagcaggaa tacaaaatgt agttaatacg attagaacaa ctttctttaa
    12721 agtagttgat gcaatcgttg gtttcgccaa agagataggc gctcaattag cctctttctg
    12781 gaaagagaac ggctcagaaa taacacaagc tttgcaaaat atagctggtt tcattaaagc
    12841 aacctttgaa tttattttta actttattat taaaccaatc atgtttgcga tttggcaagt
    12901 gatgcaattt atttggccgg cggttaaagc tttgattgtc agcacttggg aaaatatcaa
    12961 aggtgtaata caaggtgctt taaatataat acttggcttt attaagttct tttcaagttt
    13021 attcactggt aattggcgag gtgtttggga cggtattgtg atgatactaa aaggcactgt
    13081 gcagttaatt tggaatttaa tacaactgtg gtttgtaggt aagattctag gtgttgttag
    13141 atactttggt ggattgctta aaggtttaat atccggtatc tggggtgtta tcaaaggtat
    13201 tttcacaaaa tcattatctg caatttggaa tgcaacgaaa agtatttttg gtttcttata
    13261 caatagtgtt aaatctattt tcactaatat gaaaaactgg ttatctagta cgtggaataa
    13321 tatcaaaagc aataccgtcg gcaaggctca ttcgttattt acgggtgtaa ggtctaaatt
    13381 cacaagttta tggaatgcga cgaaagatat atttactaaa ttaagaaatt ggatgtcaaa
    13441 catctggaac tctattaaag ataacacggt aggtatagct ggtcgtttgt gggataaagt
    13501 gcgtaacatc tttggaagca tgcgtgacgg tttaaaatct atcattggta aaattaaaga
    13561 tcatatcggt ggtatggtag acgctgttaa aagaggtctt aataaattaa ttgaaggttt
    13621 aaactgggtc ggtggtaagt tgggtatgga caaaataccg aagttacaca ctggtactga
    13681 acatacgcat actactacaa gattagttaa gaacggtaag attgcgcggg atacgttcgc
    13741 tacggttggg gataaaggac gtggaaatgg tccgaatggt ttcagaaatg aaatgattga
    13801 attccctaat ggcaaacggg tacttacgcc taatacagat acgacagcgt acttacctaa
    13861 aggttcaaaa gtatataacg gcgcacaaac ttattcaatg ttaaatggaa cgcttccaag
    13921 atttagcata ggtactatgt ggaaagatat taaatccggt gcatcatcgg catttaactg
    13981 gacaaaagat caaataggta aaggtacaaa gtggcttggc gataaagttg gtgatgtcat
    14041 ggactttatc gataatccag gcaaactttt aaattatgta cttcaagcgt ttggagttga
    14101 tttcagttct ctaactaaag gtatgggtat tgctggcgat ataacaaaag ctgcatggtc
    14161 taagattaag aaaagtgcaa tcaagtggct tgaggatgct ttcgcagagt cgggtgatgg
    14221 cggtgtatta gatatgaata aattacgtta cttatacggt cacactgctg cttatacacg
    14281 agaaaccgga cgcccattcc atgaaggtct ggattttgat tacatttacg aacctgttcc
    14341 atcaaccatt aatggtagag cacaagttat gccttttcat aatggtggtt atggaaaatg
    14401 ggtgaaaatt gtaaagggcg ccttagaagt tatttatgca catttatcta aatataaagt
    14461 taaaactggt caacaagtta gggtcggcca gactgttggt atatcgggga atacggggtt
    14521 tagtacagga cctcacttac attatgagat gcgttggaat ggaagacata gagacccgtt
    14581 accgtggtta agaaagaata atgggggcgg caaaagtaca cccggtggta atggtgcagc
    14641 taatgctaga cgagctatta aggctgctca aaatatttta ggaggaaggt ataaggcgag
    14701 ttggattact aacgagatga tgcgtgttgc gagtcgtgaa tccaattata cagctaatgc
    14761 agtcaataat tgggatagca acgcaagagc tggtatacct tcaagaggta tgttccaaat
    14821 gatagatcct tcatttagag cgtacgcaaa gtcgggttac aataatcctc tcaacccaac
    14881 tcatcaagct atatcggcta tgagatatat tgtgggtaaa tgggtaccaa gaacaggctc
    14941 atggagagct gcgttcaaac gcgctggtga ttacgcatat gctactggtg gcaaagtcta
    15001 taacggattg taccacttag gggaagaagg atatccagag tggataatac ctactgatcc
    15061 aagtagagcg aacgaagcac acaaattatt agctttagct gctaacgata ttgataaccg
    15121 ctctaaaaat aagcgaccaa acaacttacc aaatccaagt ataagtaata gtgatacaaa
    15181 ctatattcat acattggaga ataaactgga tgcggttatt aattgtttgg ttagtttggt
    15241 tgagtctaat caagttattg cagataagga ttacgaacca gttattaata agtatgtgtt
    15301 tgaagatgag gtaaataatt ctatcgataa acgagagcgt cacgaatcta caagagttag
    15361 atttagaaga ggaggcacga taatctaatg caagatacaa ttcaaataga caataaaacc
    15421 attgaatggt tagttgtaca aagagggttt gagataccct cttttaattt tgttactgaa
    15481 aaagaaagtg tgaaaggtag aacaggttct attgctaaag ctcgttatct aaatgatatc
    15541 gaatttgaat tacctctaat aattagaaat gaagtcttgg cgccaggtgg acaaaaaacg
    15601 catgacgata tattggaaga gttagttgaa ttttttgata ttgacaattt aaagccgaaa
    15661 aaacttaaat tcaaatctca aaactggtat tggtttgcat actttgatgg accattaaaa
    15721 ttaccgaaaa acccaagagg ttcagtgaag ttcactatca aagtagtact aacagaccct
    15781 tacaaatatt cagtaacagg aaataaaaat actgcgattt cagaccaagt ttcagttgta
    15841 aatagtggga ctgctgacac tcctttaatt gttgaagccc gagcaattaa accatctagt
    15901 tactttatga tcactaaaaa tgatgaagat tattttatgg ttggtgatga tgaggtaacc
    15961 aaagaagtta aggattacat gcctcctgtt tatcatagtg agtttcgtga tttcaaaggt
    16021 tggactaaga tgattactga agatattcca agtaatgatt taggtggtaa ggtcggcggt
    16081 gactttgtga tatccaatct tggcgaagga tataaagcaa ctaattttcc tgatgcaaaa
    16141 ggttgggttg gtgctggcac gaaacgaggg ctccctaaag cgatgacaga ttttcaaatt
    16201 acctataaat gtattgttga acaaaaaggt aaaggtgccg gaagaacagc acaacatatt
    16261 tatgatagtg atggtaagtt acttgcttct attggttatg aaaataaata tcatgataga
    16321 aaaataggac atattgttgt tacgttgtat aaccaaaaag gagaccccaa aaagatatac
    16381 gactatcaga ataaaccgat aatgtataac ttggacagaa tcgttgttta tatgcggctc
    16441 agaagagtag gtaataaatt ttctattaaa acttggaaat ttgatcacat taaagaccca
    16501 gatagacgta aacctattga tatggatgag aaagagtgga tagatggcgg taagttttat
    16561 cagcgtccag cttctatcat agctatctat agtgcgaagt ataacggtta taagtggatg
    16621 gagatgaatg gattaggttc attcaatacg gagattctac cgaaaccgaa aggcgcaagg
    16681 gatgtcatta tacaaaaagg tgatttagtg aaaatagata tgcaagcaaa aagtgttgtc
    16741 atcaatgagg aaccaatgtt gagcgagaaa tcgtttggaa gtaattattt caatgttgat
    16801 tctgggtaca gtgaattaat catacaacct gaaaacgtct ttgatacgac ggttaaatgg
    16861 caagatagat atttatagaa aggagatgag agtgtgatac atgttttaga ttttaacgac
    16921 aagattatag atttcctttc tactgatgac ccttccttag ttagagcgat tcataaacgt
    16981 aatgttaatg acaattcaga aatgcttgaa ctgctcatat catcagaaag agctgaaaag
    17041 ttccgtgaac gacatcgtgt tattataagg gattcaaaca aacaatggcg tgaatttatt
    17101 attaactggg ttcaagatac gatggacggc tacacagaga tagaatgtat agcgtcttat
    17161 cttgctgata taacaacagc taaaccgtat gcaccaggca aatttgagaa aaagataact
    17221 tcagaagcat tgaaagatgt gttgagtgat acaggttggg aagtttctga acaaaccgaa
    17281 tacgatggct tacgtactac gtcatggact tcttatcaaa ctagatatga agttttaaag
    17341 caattatgta caacctataa aatggtattg gatttttata tagagcttag ttctaatacc
    17401 gtcaaaggta gatatgtggt actcaaaaag aaaaacagct tattcaaagg taaagaaatt
    17461 gagtatggta aagatttggt tgggttaact aggaagattg atatgtcaga aatcaaaaca
    17521 gcattaattg ctgtgggacc cgaaaatgac aaaggaaagc gtttagagtt agttgtgact
    17581 gatgacgaag cacaaagtca attcaactta cctacccgtt atatttgggg aatatacgaa
    17641 cctcaatcag atgatcaaaa tatgaatgaa acacggttgc gttctttaga caaaacagag
    17701 ttaaataaac gtaagtcggc agttatgtca tatgagatta cttctactga tttggaagtt
    17761 acgtatccgc acgagattat atcaattggt gatacagtca gagtaaaaca tagagatttt
    17821 aacccgccat tgtatgtaga ggcagaagtt attgccgaag aatataacat aatttcagaa
    17881 aatagcacat atacattcgg tcaacctaaa gagttcaaag aatcagaatt acgagaagag
    17941 tttaacaagc gattgaacat aatacatcaa aagttaaacg ataatattag caatatcaac
    18001 actatagtta aagatgttgt agatggtgaa ttagaatact ttgaacgcaa aatacacaaa
    18061 agtgatacac cgccagaaaa tccagtcaat gatatgcttt ggtatgatac aagtaaccct
    18121 gatgttgctg tcttgcgtag atattggaat ggtcgatgga ttgaagcaac accaaatgat
    18181 gttgaaaaat taggtggtat aacaagagag aaagcgctat tcagtgaatt aaacaatatt
    18241 tttattaatt tatctataca acacgctagt cttttgtcag aagctacaga attacttaat
    18301 agcgagtact tagtagataa tgatttgaaa gcggacttac aagcaagttt agacgctgtg
    18361 attgatgttt ataatcaaat taaaaataat ttagaatcta tgacacccga aactgcaacg
    18421 attggtcggt tggtagatac acaagcttta tttcttgagt atagaaagaa attacaagat
    18481 gtttatacag atgtagaaga tgtcaaaatc gccatttcag atagatttaa attattacag
    18541 tcacaataca ctgatgaaaa atataaagaa gcgttggaaa taatagcaac aaaatttggt
    18601 ttaacggtga atgaagattt gcagttagtc ggagaaccta atgttgttaa atcagctatt
    18661 gaagcagcta gagaatccac aaaagaacaa ttacgtgact atgtaaaaac atcggactat
    18721 aaaacagaca aagacggtat tgttgaacgt ttagatactg ctgaagctga gagaacgact
    18781 ttaaaaggtg aaatcaaaga taaagttacg ttaaacgaat atcgaaacgg attggaagaa
    18841 caaaaacaat atactgatga ccagttaagt gatttgtcca ataatcctga gattaaagca
    18901 agtattgaac aagcaaatca agaagcgcaa gaagctttaa aatcatacat tgatgctcaa
    18961 gatgatctta aagagaagga atcgcaagcg tatgctgatg gtaaaatttc ggaagaagag
    19021 caacgcgcta tacaagatgc tcaagctaaa cttgaagagg caaaacaaaa cgcagaacta
    19081 aaggctagaa acgctgaaaa gaaagctaat gcttatacag acaacaaggt caaagaaagc
    19141 acagatgcac agaggaaaac attgactcgc tatggttctc aaattataca aaatggtaag
    19201 gaaatcaaat taagaactac taaagaagag tttaatgcaa ccaatcgtac actttcaaat
    19261 atattaaacg agattgttca aaatgttaca gatggaacaa caatcagata tgatgataac
    19321 ggagtggctc aagctttgaa tgtggggcca cgtggtatta gattaaatgc tgataaaatt
    19381 gatattaacg gtaatagaga aataaacctt cttatccaaa atatgcgaga taaagtagat
    19441 aaaaccgata ttgtcaacag ccttaattta tcaagagagg gtcttgatat caatgttaat
    19501 agaattggaa ttaaaggcgg tgacaataac agatatgttc aaatacagaa tgattctatt
    19561 gaactaggtg gtattgtgca acgtacttgg agagggaaac gttcaacaga cgatattttt
    19621 acgcgactga aagacggtca cctaagattt agaaataata ccgcaggcgg ttcactttat
    19681 atgtcacatt ttggtatttc aacatatatt gatggagaag gcgaagacgg aggttcatcc
    19741 ggtactattc aatggtggga taaaacttac agtgatagcg gtatgaatgg cataacaatc
    19801 aattcttatg gcggtgtagt cgctttaaca tctgactaca atcgaattat tatcgattca
    19861 tatgcttcag ctaatattga aagtagagaa gcgccgatat atttatctcc gaacaccaaa
    19921 aataaacctg gtttaaaccg attcgcattc acattatcaa acgctgatag tgcatacgaa
    19981 actgacggtt atatcatgtt tggttcagat gaaaactata agtacggtgc tggattaaga
    20041 ttttctaaac gtagcaataa aggattggtt caagtcgtta atggtgacta tgctacaggc
    20101 ggagacacta caattgaatc aggtatgggc aaattcaact tagttaaacg aagagatgga
    20161 aatagttacg ttagcattca aagttatgat ttattggcgg taggttctga taatgctggc
    20221 gatagagtcg cttctaattc tatttataag cgtacttatt cagcacctgc taacttacac
    20281 attacttctg ctggaacaat tgggcgtgct acttctgcca aaaagtataa aatttcaatc
    20341 gaaaaccaat acatcaatga agacgatcag ttcagtcatt caaaagagat tttaaagctt
    20401 ccaattcgta catggtttga caaatatgaa tcggaaataa tggctaaaga attggaaagt
    20461 ggtaaaaagt tatctgatga tacttttaaa cttagtcgac atactggctt aatagcggaa
    20521 gaggttgaag aattaggatt taatgaattt gttatttatg atgacaacgg agaaatcgaa
    20581 ggtatcgcat acgatagact ttgggttcat ttaataccta ttattaaaaa ccagcaatca
    20641 aaaatcgaaa aactggagga attaataaat gaatgatagc aatcaaggtt tacaagccaa
    20701 tccacaatat acaattcact atttatcgca agaaatcaca agactaacac aagaaaatgc
    20761 aatgttaaaa gcatatatac aagaacaaaa tgaaaaaagc aaaagtgctg aggaagagta
    20821 atccttggca ctatttttat acaaaaattt aaggaggtca tttaatatgg caaatgaaat
    20881 tatcaaaaaa acagagagat ttattttagt acaaattgac aaagagggaa cagagcgcgt
    20941 tttgtatcaa gattttgtag gcagttttac aacgtccgat tcagcaagtt atgcacaaga
    21001 ttttaaatct gaggaaaacg ctaaaaagat tgctgaaact ttaaatcttt tatatcaatt
    21061 aacaggcaat caaaacggtg tgaaagttgt gaaagaagtt gtggatagaa ctgacttgtc
    21121 atctgataaa tcagttgata gcgaaacaat gtaactatac taagttatga gcattacgct
    21181 catagctttc ttagaaagta ggtgtagttt tggatgatat tcagaaaata aaaaaagagc
    21241 tttctgaatt agttgaacgt gttgatgatg ttgaaatact agcaaacgaa acagctgatc
    21301 atgtgcttga acttagagag gaacataagc aacatcataa tgaactaaga gaatctcata
    21361 aagaacttaa agataagcaa gataaagttg tagatgagaa tttagagcaa acaaagatat
    21421 taaacagaat tgaagaaaga tatcaaacgc aagtagatgt tgcgcaaaaa aatgaagaaa
    21481 agacactcgc ccaaaataaa tggctcgtag gtgccatatg ggcgcttgta acaattgtta
    21541 tgattgcagt cattactgca tcaattactg cgttattacc ttaagggagg tggacataat
    21601 gagttgggca agatggttat catgttattt gtatggtcgt aaatgtaaat aatgtttttg
    21661 gtcagtgcat cggcactggc tttttatttt gattgaaaag aggtacgtac atggtattac
    21721 acagctcaaa agacaggaag catactgcaa gtgaagttgg gaagtgttgt taataccaag
    21781 taagtaagat atctgaaatg tataatagag taaaaatgaa atctttttat tatagacaaa
    21841 tataaaaagt gtatagtaat atatgtatgt ataattaaat gataatcatt tcataattat
    21901 tgtatataac taaataacta cttaacaaaa ataattatgc tttagaggtg agcaaaatga
    21961 aaaaaacagc atttacatta cttttattca ttgccctaac gttgacaaca agtccacttg
    22021 taaatggtag cgagaaaagc gaagaaataa atgaaaaaga tttgcgaaaa aagtctgaat
    22081 tgcagggaac agctttaggc aatcttaaac aaatctatta ttacaatgaa aaagctaaaa
    22141 ctgaaaataa agagagtcac gatcaatttt tacagcatac tatattgttt aaaggctttt
    22201 ttacagatca ttcgtggtat aacgatttat tagtagattt tgattcaaag gatattgttg
    22261 ataaatataa agggaaaaaa gtagacttgt atggtgctta ttatggttat caatgtgcgg
    22321 gtggtacacc aaacaaaaca gcttgtatgt atggtggtgt aacgttacat gataataatc
    22381 gattgaccga agagaaaaaa gtgccgatca atttatggct agacggtaaa caaaatacag
    22441 tacctttgga aacggttaaa acgaataaga aaaatgtaac tgttcaggag ttggatcttc
    22501 aagcaagacg ttatttacag gaaaaatata atttatataa ctctgatgtt tttgatggga
    22561 aggttcagag gggattaatc gtgtttcata cttctacaga accttcggtt aattacgatt
    22621 tatttggtgc tcaaggacag tattcaaata cactattaag aatatataga gataataaaa
    22681 cgattaactc tgaaaacatg catattgata tatatttata tacaagttaa acatggtagt
    22741 tttgaccacg taatgttcag attattatga accgagaata atctaaaagt ttacaagcag
    22801 taaaaaaagt atatgtgcta taatatgctt tgagcaagtt ggatagatgg tggctatctg
    22861 agtataagga ggtggtgcct atggtggcat tactgaaatc tttagaaagg agacgcctaa
    22921 tgattacaat tagtaccatg ttgcagtttg gtttattcct tattgcattg ataggtctag
    22981 taatcaagct tattgaatta agcaataaaa aataaccatc gctaactttg gctggtttcg
    23041 atggttaaat ggttattaat ttaatcttta atctaaaata gccaccgtct ttttaacggg
    23101 ctcactaggg taacatgttt gcgcatgttg ccctttttct atatataaat taacacacca
    23161 taatataaat atcaaataga cggcttattg tctttttatt ttggataaaa ggagataaga
    23221 atatgattaa ttggaaaatt agaatgaaac aaaaatcatt ttgggtagcg atattgtcag
    23281 ctatcttttt atttgctcaa aacatcgcca aagctattgg gtatgatatt caagtttata
    23341 cagagcaatt aacagacggt ttaaacgcta tattaggatt tttagtatta actggtgtga
    23401 ttcaagaccc gactactaaa ggtataggtg atagccacca agctttagaa tatgaagaac
    23461 caagaagaaa atactaggag gtaaaataat gaaaacatac agtgaagcaa gagcaaggtt
    23521 acgttggtat caaggtagat atattgattt tgacggttgg tatggttacc aatgtgcaga
    23581 tttagcagtt gattacattt attggttgtt agaaattaga atgtggggaa atgcaaaaga
    23641 tgcaatcaat aacgatttta aaaacatggc aacagtatat gaaaacacac catcgtttgt
    23701 tccacaaata ggtgatgtgg ctgtatttac caaaggaata tataaacaat acggtcatat
    23761 tggtttagtg tttaatggtg gtaatacaaa tcaattttta attttggaac agaactatga
    23821 cggtaacgca aatacgcctg caaagttacg ttgggataat tattacggct gtactcactt
    23881 tattagacct aagtataaaa gtgagggctt aatgaataag atcacaaata aagttaaacc
    23941 acctgctcaa aaagcagtcg gtaaatctgc aagtaaaata acagttggaa gtaaagcgcc
    24001 ttataacctt aaatggtcaa aaggtgctta ttttaatgcg aaaatcgacg gcttaggtgc
    24061 tacttcagcc actagatacg gtgataatcg tactaactat agattcgatg ttggacaggc
    24121 tgtatacgcg cctggaacat taatatatgt gtttgaaatt atagatggtt ggtgtcgcat
    24181 ttattggaac aatcataatg agtggatatg gcatgagaga ttgattgtga aagaagtgtt
    24241 ttaattctta ggttaaaatg tt
//
LOCUS       DBNENW010000025        22953 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNENW010000025 DBNENW010000000
VERSION     DBNENW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.025"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..1167)
                     /gene="tarF"
                     /locus_tag="AC1LW8_000803"
     CDS             complement(<1..1167)
                     /gene="tarF"
                     /locus_tag="AC1LW8_000803"
                     /EC_number="2.7.8.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid glycerol-phosphate transferase
                     TarF"
                     /protein_id="HIP4772660.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDRILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
     gene            complement(2193..3887)
                     /gene="tarK"
                     /locus_tag="AC1LW8_000804"
     CDS             complement(2193..3887)
                     /gene="tarK"
                     /locus_tag="AC1LW8_000804"
                     /EC_number="2.7.8.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid ribitol-phosphate polymerase TarK"
                     /protein_id="HIP4772661.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWIHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3909..4934)
                     /locus_tag="AC1LW8_000805"
     CDS             complement(3909..4934)
                     /locus_tag="AC1LW8_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIP4772662.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LPSSYFRSSGYDGFMQDYVVMAHDRIVPLPNAIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKINHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4927..5642)
                     /locus_tag="AC1LW8_000806"
                     /pseudo
     CDS             complement(4927..5642)
                     /locus_tag="AC1LW8_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
     gene            complement(6170..7213)
                     /locus_tag="AC1LW8_000807"
     CDS             complement(6170..7213)
                     /locus_tag="AC1LW8_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HIP4772663.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(7237..7383)
                     /locus_tag="AC1LW8_000808"
     CDS             complement(7237..7383)
                     /locus_tag="AC1LW8_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772664.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(7385..8440)
                     /locus_tag="AC1LW8_000809"
     CDS             complement(7385..8440)
                     /locus_tag="AC1LW8_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIP4772665.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(8458..9717)
                     /locus_tag="AC1LW8_000810"
     CDS             complement(8458..9717)
                     /locus_tag="AC1LW8_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HIP4772666.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9944..10222)
                     /locus_tag="AC1LW8_000811"
     CDS             complement(9944..10222)
                     /locus_tag="AC1LW8_000811"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP4772667.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(10245..10712)
                     /locus_tag="AC1LW8_000812"
     CDS             complement(10245..10712)
                     /locus_tag="AC1LW8_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP4772668.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10697..12793)
                     /locus_tag="AC1LW8_000813"
     CDS             complement(10697..12793)
                     /locus_tag="AC1LW8_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP4772669.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQLEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12924..13020)
                     /locus_tag="AC1LW8_000814"
                     /pseudo
     CDS             complement(12924..13020)
                     /locus_tag="AC1LW8_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13130..14065)
                     /locus_tag="AC1LW8_000815"
     CDS             complement(13130..14065)
                     /locus_tag="AC1LW8_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIP4772670.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            14427..15956
                     /locus_tag="AC1LW8_000816"
     CDS             14427..15956
                     /locus_tag="AC1LW8_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIP4772671.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            15983..16108
                     /locus_tag="AC1LW8_000817"
     CDS             15983..16108
                     /locus_tag="AC1LW8_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772672.1"
                     /translation="MATMYEVRSDIVAFIFNVTFEALSSSCTVVSPQIASARQSS"
     gene            complement(16276..17229)
                     /locus_tag="AC1LW8_000818"
     CDS             complement(16276..17229)
                     /locus_tag="AC1LW8_000818"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIP4772673.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17540..17647)
                     /locus_tag="AC1LW8_000819"
     CDS             complement(17540..17647)
                     /locus_tag="AC1LW8_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772674.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17802..18947
                     /locus_tag="AC1LW8_000820"
     CDS             17802..18947
                     /locus_tag="AC1LW8_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HIP4772675.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSEKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18973..19146
                     /locus_tag="AC1LW8_000821"
     CDS             18973..19146
                     /locus_tag="AC1LW8_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772676.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            19303..19659
                     /locus_tag="AC1LW8_000822"
     CDS             19303..19659
                     /locus_tag="AC1LW8_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIP4772677.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            19857..21332
                     /gene="nikA"
                     /locus_tag="AC1LW8_000823"
     CDS             19857..21332
                     /gene="nikA"
                     /locus_tag="AC1LW8_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772678.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANAKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21646..22788)
                     /locus_tag="AC1LW8_000824"
     CDS             complement(21646..22788)
                     /locus_tag="AC1LW8_000824"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIP4772679.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMLLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
BASE COUNT         7354 a         4323 c         3518 g         7758 t
ORIGIN      
        1 cttaaaaatc gtttggcaaa ttcgttgtga cgcgtgtcca tcttctaatg aacagaattt
       61 ttgataaaat accgttcgtg cttcaatata ctcatttgca gtctcatcga tttgttttaa
      121 tgcatcaatg agtgctgttt gattttcaac aattggacct ggcaactctt ttttataatc
      181 catgtaaaaa cctctaagct catcgccata tttatctaag tcatacgcat agaaaatttg
      241 cggacgcttt aatacaccga agtcgaacat gacagatgag tagtcggtaa ctaacgcatc
      301 gctgattaag tataaatccg aaatatcttc ataatctgaa acgtctttca caaaatcatc
      361 atgttcatca atacgtgtca caactaaata atgcatgcgt aataaaataa cataatcatc
      421 atccagcgct tgacgcaaag cttctatatc aaagttaaca ttaaattgat atgaaccttc
      481 tcgaatcgct tcatcgtcac gccaagttgg cgcgtacata atcacttttt tatctaatgg
      541 aatatttaat cttgtcttaa taccattaat atattcagta tcattgcgtt tatgtgataa
      601 tttatcattt cttggataac ctgtttccaa aatcctatct cgactaacat gaaatgcatg
      661 ttgaaatatc gatgtcgaat atggattagg tgacactaga taatcccacc gttggctttc
      721 ttttttaaag ccatcttggt aattttgagt atttgttcct agcattttaa cgttactaat
      781 atccaaacca atctttttca atggcgtgcc atgccatgtt tgtaagtacg tcgttcgcgg
      841 tgatttatat aaccaatctg gtgtacgtgt gttaatcatc cacgctttcg ctcttggcat
      901 cgctaaaaac catttcattg aaaactttgt aacatatggt acattgtgct gttggaatat
      961 gtgttcatat ccttttttca caccccatat taattgggca tcgctatgtt cagttaagta
     1021 ttcatataat gctttggggt tgtcgctgta ttgtttacca tgaaagcttt caaaataaat
     1081 tagattcttg tttggcaatt ttgatagtaa tttaaaagtc gtatatatac tatgttctat
     1141 caatttttta attgtatttt taatcatgtc gtacctccga cgtgtttttg taattatatt
     1201 aatatgtatg agcaagctca ttgtaaccat gcctattata gcatttcatc ataaaataca
     1261 tttaaccatt acacttgtcg ttaattatca tacgaaatac atgattaatg taccacttta
     1321 acataacaaa aaatcgttat ccattcataa cgtatgtgtc tacacattta tgaattagat
     1381 aacgattgga tcgattattt tattttacaa aatgacaatt cagttggaac aggctgggac
     1441 ataaatcaat gttctatgct ctacgaagtt atattggcag tagttgactg aacgaaaatg
     1501 cgcttgtaac aagctttttt caattctagt caggggcccc aacacagaag ctgacgaaaa
     1561 gtcagcttac aataatgtgc aagttgggga tgggccccaa cacatagaat ttcgaaaaga
     1621 aattctacag gcaatgcaag ttggggtgtg ggccccaaca cagaaaattc attttattga
     1681 attttacatt tatgtgcaag ttgggcaaag tatcttattt ttcaaagtat ttaaaagtaa
     1741 aattacatgt taatacgtag tattaatggc aagactcctg agggagcagt gccagtcgaa
     1801 gacaggggcc ccaacacaga agctgacgaa aagtcagctt acaataatgt gcaagttggg
     1861 gtggctgaga cggcacccta ggaagggacc cgtcatcaaa aattctattt atagaatttt
     1921 acagtaatgt gccagacggg caaagcgaag ccattcaata cgaagtattg tataaataga
     1981 gaacagcagt aagataattt tcaattagaa aatatcttac tgctgttttt tagggatttc
     2041 tgtcccagcc tcttatgcat gaaaaatcaa aaggttattc tcattgtata gtcctgcttc
     2101 tcatcatgac atgttgctaa cttcattgtc agaacccttc ttgaaaacta tgccttatga
     2161 ctcatttgca tggcaagtaa tatatgccaa cattagcgtc taaacaaatc tttgactaaa
     2221 cgttcacttg agcgaccatc ttgatattta aaatgtttat ctaagaatgg tacaactttt
     2281 tcaacctcat aatcttcatt gtccaaagca tccattaatg catcaaagga ttgtacaatt
     2341 ttacctggaa caaatgattc atatggttca tagaaatcac gcgtcgtaat gtaatcttct
     2401 aagtcaaatg catagaaaat catcggcttt ttaaatactg catattcata tattaaagat
     2461 gaataatcac taatcaacaa gtctgtaaca aagagaatat cgttaacttc acgatgatct
     2521 gacacatcga taaagtattg tctatgttca cgtgaaatat taagtctatt ttttacgaac
     2581 ggatgcattt tgaataacac aactgcatta tgcttctcgc agtatcttgc taaacgttca
     2641 aaatcaattt taaaaaatgg ataatgtgcc gtaccgtgac cattacctct aaatgtcggt
     2701 gcgaatagaa taactttctt accttttata attggcaatt catcttccat ctcttgttta
     2761 atttgtgttg cataagcttc atcaaatagt acatcagtac gtggtacacc tgttggtaca
     2821 acattttctt ctctaattcc aaatgcttca gcataaaatg gaatatcggt ttctgatgaa
     2881 acatatgctt tcgtgtaact acgatggttt aatgaatcga taaatggacc acctttttta
     2941 cctgtacgac taaagccaac tgttttaaaa gcaccaacgg catgccacac ttgaataatt
     3001 tcttgtgatg gtctaaagcg aaccgtataa attaatggat ggaaatcatc aacaaaaata
     3061 taatctgcct taccaagtaa atatggcaat ctaaacttgt ctctccattt acgtctatcc
     3121 gtaatattcg ccttaaatac cgttttaata tcataatcaa aatctacttt ttggcgtagt
     3181 aactcatcat atacatactt gaaattccct gataaattcg gacgcgaatc tgatgtgaat
     3241 aatattgttt tgcttctttt aatatgtagt aactttgtaa tattaaaaat agctttaaat
     3301 aagaaacttc tactttcaaa tgaagcttta tgaccttgtt tatgaatcca gtgtgcactt
     3361 gtcgcaatga cccctgattt ctcttgaggt aaggtgattt caatatcaaa tacaaattcg
     3421 ttaacgtcac ttgctatttc cggagtaatg gtatagaccg tatgttgtga tacgccacct
     3481 ttacggaaaa ctttagcatc atacgctaat aaatagttca cttttccatt ttgtgtagtc
     3541 agctcattgt attcttcaat ttgctcttca tttaatgccg cataagcttg atcaataata
     3601 tttggattaa gttgtccaat atactctaac tggtcatgat aaacaaataa atatttatcc
     3661 attggtaaat aatacccatc gtctaatatt gcgacgttga atctacatat aaatgtatta
     3721 ccgtctattt tcatttcatt tgcttctaat gtttttgttt cggttaaatt cctcagaaca
     3781 aattgtcctg atgttaaatc gacaccttca ctatgtcctt caatatataa ctgaacacgt
     3841 tcccagtata tgttatcaat atgtattgct tgtttcgttt ttgtcattgt tattccccca
     3901 tcggtttatt aaatcgtcca tttcaatact gtttttcccc aagatgtcga taaatccatt
     3961 tcaaacgctt ggacgatatc ttgcatcgta catacattaa tttcatgtcc ttttaataag
     4021 gctaactttt caactatgtc tgggtactta cgatataaat caacaacttg ctcaaaatct
     4081 ttagagccgc ttcgactact accaatcaat gttaatcctt tttcaagtac taatcgtgta
     4141 ttcacttcca cgggtaattc acttacgcct aacaaagcaa tactgccttc tggtgaaata
     4201 tgttcaacta tttgttgaag tgcaacttga cttcctttac ctccaacaca ttcaaatgca
     4261 tgattaattt taagatcatc tggtatttga tttactgtaa agatgtcatc tacaaatgaa
     4321 aaatgactta atttatagtc tgtcttacca aatacataaa ttttagcttc tgggtacaac
     4381 ttacgtagca aaatagcagt aatataacct aagttaccat caccccaaat accaaagctg
     4441 gttttcaaag gtatagattt acgttcaaat cgttgtatag catgataact tactgacact
     4501 aactctgtgt atgaaatcgt actcaaatca atggcattag gcagcggaac gatacgatca
     4561 tgtgccatca caacgtagtc ttgcataaaa ccatcataac cactagatct aaaataacta
     4621 gagggcaggt aattctcggc aatcacgtcg tgtttttctg taggtgtatt cggcaccatt
     4681 actactttcg tacctttttc aaatacgcct ttactatcga atacaacttc accaacagct
     4741 tcatgaacta gtgacattgg taattttttg cgcagtacat tttcatctct tcgacctgtg
     4801 taataccttt gatcagctgc acaaatagac aagtataaag gtcttacaat gacatgatta
     4861 ccataaatat caacattatt atatgtgacg tcgaactgtc tcggtgcaac gagttgatat
     4921 acttgattaa tcatcggcaa tatcaccttg aataatggca tttgctactt ttaaatcata
     4981 cggtgttgca ctttaatgtt gtatagttct ccacgtacca atttaactgg atgtccagat
     5041 tcgacaatga ttttacatgc atctgataag atttcttttt gtgcactact taaggcgcga
     5101 taactatctt gtaataattt aatattaaat gattgtggtg tttggccttg atacatttca
     5161 ttccttacag ggatactgtg tatgttctgt ttatctttag acattacaat cgtatcaatt
     5221 gcttcaatga ctgtatctac tgcaccatat ttttctgcta cttcaatatt ctctttaata
     5281 atacgttgag ttaaaaatgg tcttacggca tcatgagtta caatcacatc atcattatta
     5341 attccattta cattgcgaat atagtcgata atgttcataa ttgtttcgtt tcgatccgta
     5401 ccacctgcaa ctactttgac acgttgatct gtaatgttat atttttttaa aatatcctgt
     5461 gtatgggaaa tccactgtgc tggcgttgcg ataataatct cattaaattc actcactaaa
     5521 atgaacttct caattgtatg gattaaaatc ggtttattat caatatctaa aaattgtttt
     5581 agtaatggca cgttccccat tctcgaacca atacctcctg ctaaaatacc tgcataaatc
     5641 atgttgtcct ccattctgtc attacatcat ttccatttat acattactga cctatgcccg
     5701 cacataagcc taacctattg ctcacttgcc tcttttatta atccaaagat agttgtcaca
     5761 atagtgtgat aattttttat aaaaatgtat ttttgtaact gaccattcta agttgttttg
     5821 ccatgcagtt aatcattaac tctgacgata ttaaattgtt aaaggtatta atgtttactc
     5881 tttttcaaat tcattattac tgccatcatt ttaccatata ttataataaa tttatcttat
     5941 taagtggctg tacttgattt tcactttaaa aattatcaaa tattgccatc tcattttaag
     6001 tatacaaaat gcaaaacaac cgattcacaa gcatatttca cacaagtaaa ccggctattt
     6061 agcaacgtat attcgaagat gagttatttc gatagtatct gtagaccaga cggcattcgc
     6121 actttcatag ctataactat accagtgttt tcgtcctcaa aggtgcatac taataaatcg
     6181 taaacatgac tttatcaaat cgttctttct tgttaactaa tttatcaaat gtctctgggc
     6241 ctttttctaa cggtaaaaaa tgagaaataa taggctttac attaatatct ttcgtcttca
     6301 tataatgtaa ggttgccgtc cactctttgc ccggaaaatt actggacaaa cagttccaag
     6361 agccacatac tgtcaactcg ttacgcagaa ttttttcaaa atgaacgcga tcaatctcaa
     6421 tatcatcata tggtattccg agtaatacca cctcgccacc ttttttaggt agcgtcaata
     6481 tttgaccaat cgtaacttta gcacctgatg attctatagc taaatcgatt tgattggcgt
     6541 aatgattttc gatgaatttc tcaagatttt cttcttttga attgattgtt tgatgtgcgc
     6601 ccaatgatgt tgcaatatct agtttatgcg catctatatc tatagcgatg atatgtgcag
     6661 caccaaatat tcgtgcccat tgaatagcta acaaacctat actgccacac cccattactg
     6721 caacagtcat accaggttgt atattcgatt tataaaaccc atgcgcaaca acggctgatg
     6781 gctcaaccat tgctgcttca atgtaatcaa cattgtctgg aacctttaaa acattttgcg
     6841 ctggcaattt gacatattcc gcgaacgatc caggttcata tgagccaata acgaataact
     6901 tttcacatcg tgcatattca ccttttaaac aatactcgca ttgataacaa ggtattgctg
     6961 ggcaacctgt cactttgtcg cctacattaa catgcgtaac atcacttcca atggcatcta
     7021 caacacctga aaattcatga ccaaatggca tacctttaat gtatggcccc atttttttgt
     7081 atcgtgacgt gtctgaacca catatgccag tcgctcgtac tttaacaata acgtcattcg
     7141 cactttcaat gactggcttt tcattatcct cataccgtaa atcttccacg ccatataatt
     7201 tcaatgcttt cacttgtaaa tcacctcaaa tttgatttaa ttcacaactt ttttcttttt
     7261 aaaaatacct gtcgcaaaat aacctgcaat gacaatggaa ttacttacga gtaaatgttc
     7321 catataaaaa tcagtgattt gtcttaatgg cccaagcata aaagttagca ttgattctac
     7381 catattaatt ctctccttca ttttcatcta aatcgagcgg cttaagtaat gctttaatac
     7441 cttcgccaga ttttaaatgt tgatacgcct cgtcccattt cgaaatatca tatatttttg
     7501 tcaccaaagc ttcagcattt actaaaccat ccgccataag ttgcaatgaa ggttcccaat
     7561 ctgctggctt ttgacttcta ctaccaacaa ctgttatttc tttttgaatc actttttcca
     7621 tatcaaatgg aatttcagca tccttaaaaa tacctatttg actgtagaaa ccttttttgc
     7681 gtaaaatatc caaaccttgt cgtgctgctg gaactgcacc tgaacattca acaacaacat
     7741 ctgcaccgta accgtctgta attccattga tatacgtttt taagtctgtt tgttgtaaat
     7801 tgactacata atccatgtgc aatgcttctg ctttatctaa tctgactttg tcattgtcca
     7861 atccagttac cacaacagtt gcgcctttac tttttaacac ttgtgctaca agtaatccga
     7921 ttggcccagg tcccattaca actgctacat cgcctgaatt gacttgaatc ttagaaacgc
     7981 catgatgtgc acatgctaat ggttctgtca tagctgcaga ctgatacgat acttcatctg
     8041 gaatatgatg caaactttct tcacgtgcaa tgacataatt agtaaatgcg ccatcaactt
     8101 gtgttccaat accttttcga tggttgcata aattatagtc ttttgattta cagtattcac
     8161 actcattaca aacatagaat gtcgtttcag aagtgacacg gtcaccaact ttaaaatctt
     8221 taacgtctgc tccaacttca acgatttcac cagaaaattc atgacctaat gtcactggaa
     8281 aattaacttt ataatgccct tcataagtat gaatgtctgt gccacaaatt cctgcataat
     8341 gtactttaat ctttacttta tcatctagcg gtgttgcaac ttctttatca agaagttcta
     8401 agttgccatg tccttctctt gtttttacta aagctttcac cacaaacacc tcgattttta
     8461 attgaataga ctaaatagtt taaagataag atagttaacg atattaccac cttgatcaat
     8521 acttgaaatt tcagatgaac cttttggcat ttgtacattc gtacctttcg ccatatctgt
     8581 gaaaatgggt gctacgtctg ttgcaatata taatgaaatt gcaatcataa tcgtgcccac
     8641 aatgacagaa tgaataatgt ttcctcttgc tgcaccaaca ataaacgcga caacaaatgg
     8701 tatagttgct aagtcaccaa aaggtagtac ttggtttcct ggtaaaataa cggctaataa
     8761 aacagtgata ggtactaaaa ttaatgctgt cgaaataacc gctggatgac ctaatgctac
     8821 agccgcatcc aatccaatat aaatttcacg ttcgccaaaa cgtttattta gccatgttct
     8881 tgcagactct gaaactggca ttaaaccttc cattaagatt tttaccattc taggcattaa
     8941 taccattact gcagccattg acattcctaa attaatgatg tctccaggtt tgtaacctgc
     9001 taacacacca atacctaaac ctaaaattaa tccgacaaag atagactctc caaatgcgcc
     9061 aaaacgtttt tgaattgttt caggatcagc atctaactta ttcagaccgg gtactttttg
     9121 taacaattta actaagtaaa tacctggtgc ataagaaatt gtacttcctg tagcaatcga
     9181 tacacctggt aaatcaaaga actcactcat catcggtgct gtccaatctg ctactttcaa
     9241 acagataact tggaaaataa ctgctgctaa taacgcttgc caaatactgc ctgatacggc
     9301 ataaaccatt gctgctgtaa acgtataatg ccaaaaattc caaatatcta cattcatcgt
     9361 ctttgtcact ttagttacta gcaatacaac gttaactatg attccgagtg gaataataaa
     9421 tgctgcgaca gatgatgccc aagcgataga tgatgttgct ggccaaccta catcaatcac
     9481 attcagactg acgcctaaat ttttaaccat cgcttgtgct gctggcccta aatttttaac
     9541 taataaatcg atgactaaga aaatccctac aaaagccaca cctattgtta aaccagacct
     9601 aaatgccgct ccaattttct gcctaaagaa taggccaagc aagaatatga caaccggtaa
     9661 aataacagtt gcacctaaat ctaaaaatcc ccttacaaaa tcagtgaagt aactcatatt
     9721 taaaccctcc ctgttatata tgcattgtca cgatactttc cgattgtgat tacatttgac
     9781 gttacagtca tttcaacgac aacccttgct acattcgact gcagtcattt tgaattacag
     9841 tcactgcgtt tctatgtcat caacaatcat ttgtcgtgat agtcatttat atgcaatttg
     9901 catatattaa tatgttatcg acccacgtta catatcaatt ccgttatttt tgtaactctg
     9961 ttaagatttg ttgttttgtt tcttcaatac caataccagt taagaaatta cgtgcgttga
    10021 taactgggaa tttatattct ttctttgtca ttgcagttgt aactaataaa tctgcagtgt
    10081 cttcataagg tccaacttct gtaattttga tttgtttaat atctacttta atattgtgtt
    10141 cctttgccat ttcctcaatt gcattattta ctactgttga cgttgcaata cctgcaccac
    10201 acgctactaa tacttgtttc attttcaatt cctccaatta atttttagtt atattccaaa
    10261 taatcattga ttagtgttgc taaaatcgtt tcatctttcg ttcgcagaat ctgctccaat
    10321 ttttcttcac tttgaaaaat ttgcatcaac tgttgtaaca acttaagttg atcatctact
    10381 ttatccatcg ctaacataaa aacgattttc acttctgtct gttgatcaag tgttcccatt
    10441 tcaataaacg gcacctcttt ttctagaaca gccacaccta tcgttctatg gttaatatgt
    10501 tcgacatctg tatgcggtat agcgaccgaa catagatgtg ttggtaaacc agtagcaaat
    10561 tctttttctc tgtcgatgac tgcatcttta aacgttgact tcacgaaccc attttgaaat
    10621 aacacatctg acatttgtga caatacggat tctttatcag ttgccgacaa attgagcatt
    10681 atattttctt tatgcactaa ttgctgtccc atccattttc cctcgcttct ttatttgaat
    10741 aattttttaa aatctcattt acatcagaat ttttgcgact ttgtatgatg cgcttaattg
    10801 cgtcattgtc ttgcgccaca tctctcaatt gtagtaacgc tcttaagtgt gtcactttat
    10861 caacagcagc aataggtaca ataatatgga ttgctgtgcc atctgacatg tatattggtt
    10921 cttgtaatat caacatactc atcgctgttt tatgtacatg cttttcagag tatgcatgtg
    10981 gaatagcaat attttgcatg ataaccatat acgaatcatc aaatacctgt tgcatctcat
    11041 gaatatattt aatatcaata aaatgattag caactaacac atcacttgct ttagcaatag
    11101 cttcatcaat attttcaaca tgatgtattc ttttcacgtg ccttgccggt atcaagtcag
    11161 ctaaatctaa tgtcttattt tgtgtgacaa tcgatccatt aatggttgaa attgaattat
    11221 aattcgcaat aaaatcttct aaaccatcac gtagttctgt aatgtcatta actgtcgtag
    11281 tgcgttcaat taatgccatt aacttgttta tttccttatc aatgtcagcc gattccttat
    11341 taatgtactt catcacttct ttacgtaact ttcgttgctc attttcagat aaagctactt
    11401 ttgtgataaa taatttttta tgtgttagga caaacattgg tgaaaagacg atgtcataat
    11461 ctaatgtgta attttcaaat gttctaagtg aaatcgcatc taagaaaata atttctggaa
    11521 ataagtttcg caactcgtat aacatcattt gtgatactga cgtgccttgt gtacacacga
    11581 taatagcttt tatcttgcca tcgaagtttt catcttgacg tctcaaacta cctccgaaca
    11641 acatggttaa atatgctatt tcattatcag gcaacgattt tccgaaatat tcagttaacg
    11701 attgacatga ttgtttcacc atatgaaata aggattgata atttccttgt aaaggattta
    11761 ttaattcatc acgatccgtt aagttatatt taatcctata aaaagcaggc gttaaatgta
    11821 acaagagttg ctgtgataat ttctccttat cttcaatgtt aataaaagtg atttgttcaa
    11881 aatggtgaat catttgagcg atagccatcg ttaaattcga tatgttatct gattcctgca
    11941 aatcagtcca ttgcacactt gttgaaagta agtgtaatgt caaatataac ttttccgctt
    12001 ctggcaaatc cggctcaagt tgcgtcataa tctccgttgc ttgatattct ttcgtatccc
    12061 tcaaatactg ataattaata tttaatggat tcatcacatg accactttga attcgtctac
    12121 gaatcacaca aaggacataa ggcaatgaac taagtgattt gtctataaag cgactcttca
    12181 aaaattgttc tacctgtttg attttgtctt tttgatatgc gatatcttcg aatgttaagt
    12241 tgagcgcctt taaaacttca cttttagtaa tatcatgatt caacctttga tcaatcaact
    12301 taatgaagaa acggcgaact tcaaattcat caccaacaat ttcataacca tgttttcgag
    12361 aatacttaag tgacaaacca tgattttcca attgctcttt cacatgattt atatcgtgaa
    12421 tgacagtatt tttactgact tgtaaatcaa ttgaaaaatg gtttagagac attgcgtttt
    12481 ccttactaaa aagcatgagc attaaataat aacgacgtgt ttctatgcta aaaatgacat
    12541 tgttgccgtt taacatttgc tgctctgata catctcgttt gaataacgtc atgatttcag
    12601 aacttacaat aaaatttcct tggcttgttc tttcaagttt tggataaccc tcttgttcaa
    12661 gccacaaatt gattttttga atgcgatatc ctagttgtct acgagacaaa ccaaatatcg
    12721 attcaagttc tttaccatga atagtaggat tcaatacaat ttctctgagt atttgctgat
    12781 tatccccgtt catgttcgat cctcctttga gcatctgagc tcaatttatc atcaatcgtc
    12841 acaccgatgt acacactttg aacacatatt ttcaaaatga gcatgtacat cattgtgatg
    12901 ttttaacaac atttcaatta tatctatatt ttttgtgatt ttaatctttt aaaataaagc
    12961 aattgaaatt ttgaatatat ttttgtattt tgtgtttttt gaagcatttt taacatacat
    13021 atctcaatca ttatcaaatt gtcatgacca ttgtaatcca atacaaaaac cctaaggacg
    13081 cttatatcag gcaccttagg gctaactgta tctatttaat taagtattat tattcgtatg
    13141 tacgtaactt atggtctatc aagttccaca cttcttcaac atcaactgct gtagcaaaat
    13201 aagcattggc aggcttacct gtaacatgat ttaaatcgac agccatagtg ccataagtta
    13261 gtggactttg atgttcaatg tcgatattaa cgggtaccat tgtaaacaat tctggttgta
    13321 acaaatacaa aattgtacaa gcatcatgta ttggaccacc atccatatta aagtgagtct
    13381 tgtatgtctt cttaaagaat tgcaataatt ctacgacgaa ctgtgcaaca ggattattga
    13441 tactttcaaa gcgttcaatc acgtgatcgt cggctaaaac ttgatgtgtt acatctaaac
    13501 caaacacatt tatagtaatc ccactttcaa aaacacgctt cgctgcttca gcatctaccc
    13561 aaatattgaa ttctgctgta ggcgtccaat ttccaaatgt accaccaccc atcaaagtaa
    13621 tagattcaat atgctcagcg attcttggct cacgaatcaa tgccgttgct acattcgtaa
    13681 gaggacctgt cgctacaatt gttacaggtg tatcactcgt catcactttg tttataatca
    13741 catctgatgc tggcattgca actgcttgac gtgatggtgt cgacggtagt ttcggaccat
    13801 ctaatccaga ttccccatgt atttcagaag caaaggcagc tggtttaatt aacggcctat
    13861 ccgcaccttt cgctactgct atatcttggc gtcccataat atccaatacg ttcaaggcgt
    13921 ttgtcgtatt cttgtcaact gattgattac ctgcgactgt tgttacagct aatatctcta
    13981 gtggactgtc aattgccccc gctaaaatta atgctattgc atcatcgtgt cctggatcac
    14041 aatccataat aatctttctt ttcatttata tatccacctt tcttaagttg ttatcgatag
    14101 cttatgtata tttatttatg tggtgaatca tgtttatttt gaaaaatagt tttaactttc
    14161 tcatattttt ggatacaaac actatttatc tattttatgg cttataaatt tatccgatat
    14221 gccttatcaa cctacctcgc taaaaatagg atgtctacat atctataccg acttttgtca
    14281 actcattttc acaacaatat aaacagcaat ttatatgatt gttacatgat tcaaacaatt
    14341 tttatgaaaa atattttcat acacagaata tatattgata ttaaatttct caaaagctat
    14401 attgagaata attaggaggg atgttgatga aatctttatt tgaaaaagca cagcagttcg
    14461 gcaagtcctt tatgttacct atcgcaatct taccagctgc aggtctattg ttgggtatcg
    14521 gtggtgcatt aagtaatcca aacaccgtta aagcataccc tattttagat attaccttat
    14581 tacaaaatat ttttacatta atgtcagctg caggtagtat tgttttccaa aatttaccgg
    14641 tcatctttgc aattggtgtc gcaatcggat tatctagaag cgataaaggt actgcaggtt
    14701 tagctgcgct gctcggtttc ttaattatga acgcaactat gaatggctta ttaactatca
    14761 cgggcacatt ggcaaaagat cagcttgcac aaaatggaca aggcatggtg ctcggtatac
    14821 aaacggttga aaccggtgtt tttggcggga ttatcacagg tattatgacc gcaatacttc
    14881 acaacaaata tcacaaagtg gtattaccac cgtatttagg tttctttggt ggctctagat
    14941 ttgtccctat tgtcacagca tttgccgcaa tctttttagg tgtattgatg tttttcattt
    15001 ggccaagcat acaagccggc atttatcatg ttggtggatt tgtaacgaaa acaggtgcca
    15061 tcggtacttt tgtttatggc ttcatcttaa gattgttagg tccactcggt ttacaccata
    15121 ttttttactt accgttttgg cagacggcac ttggtggtac tttagaagtc aaagggcact
    15181 tagttcaagg tacgcagaac atcttctttg ctcaacttgg tgatccagat gtgacgaagt
    15241 attattcagg tgtgtcacgc tttatgtcag gccgttttat tacgatgatg ttcggcttat
    15301 gtggtgccgc acttgcaatt tatcacacag ctaaacctga acataaaaaa gttgtcggcg
    15361 gtttaatgtt atccgctgca ctcacttcat ttttaacagg tattaccgaa cctttagagt
    15421 ttagtttctt gtttgtcgca cctattcttt atgtaatcca tgccttcttt gatggattag
    15481 catttatgat ggcagacatt ttcaacatta caattggtca aaccttcagt ggaggcttta
    15541 tcgatttctt actctttggt gtgctacaag gtaatagtaa aacaaactac ctatacgtca
    15601 tacctattgg aattgtgtgg ttctgtttgt attacatcgt tttcagattc ttaattacga
    15661 aatttaattt caaaacacct ggtcgagaag ataaagctgc agcacaacaa gttgaggcta
    15721 ctgaaagagc acaaactatt gttgctggtt tgggaggcaa agataacatt gaaatcgttg
    15781 actgttgtgc aacgagacta cgcgtcacac ttcatcaaaa tgacaaagtc gataaagtat
    15841 tactcgaaag tactggtgcc aaaggtgtaa tccagcaagg cactggtgtg caagtaattt
    15901 atgggcctca cgttacagtt atcaaaaatg aaattgaaga attgctcggg gattaagact
    15961 aaccgaaata tcaacagaac taatggcaac gatgtacgaa gtaagaagtg acatcgttgc
    16021 ttttattttt aatgttacat ttgaagcatt aagttcatca tgcactgtag tgagcccgca
    16081 aatcgcctct gctagacaat catcttaatg ctatgattaa agcttaagtg ccagatttga
    16141 atttaatttc aacaacgact ttcactacat taaaaatagg gctactcgac acatatagtt
    16201 gtatcaaata gccctttata caattttttg ggtaaggttt tacaattttt ggaatggtat
    16261 agattttata aaaagttatt taagttcttc tgcttcagcc ataatatctt ttaatgtttt
    16321 agctgaatgt gcgaacttgc tttgttcttc gtcgtttaat gggatttcta cgacgttgcg
    16381 aataccgttt ctattgatga ctgctggaac accaatataa acatcttctt catcatattc
    16441 gccttctaat aatgctgata cagtcaatac ggcatcttca tttctgaaaa tcgcttcagt
    16501 aattctagct aatcccattg caacaccata ataagtggca cctttagctt gaataatgtc
    16561 atatgctgca tcacgtgttt gaacaaaaat ttgttcaatt tgcgctttgc cctcaggacg
    16621 ttgttcaagt aatgtcttca aaggttgacc cgcaatatta gcgtgtgacc atactggtaa
    16681 ttcagtgtca ccatgttcac caataatttg agcatcgacg ctacgtggcg caacatcgaa
    16741 cgcttcgctt aacaataatc taaagcgtgc agagtctaaa attgtaccag aacctataac
    16801 acgttcttta ggtaaaccag agaatttcca tgttgcatac gctaaaatat caacaggatt
    16861 tgtagctacc aagaaaatac catcaaattt tgatgccatt acttcaccaa caattgattt
    16921 gaatattttc aagtttttag atactaaatc taaacgtgtt tctccaggtt tttgtgcagc
    16981 accagcacag atgacaacta gatccgcatc atgacaatca ctgtattcgc cagctttcac
    17041 acgaactgtt gttggagaat atggtgtggc atgttttaaa tccataacat ctcctcgaac
    17101 tttttcagtg tctaaatcaa tgatgactaa ttcatcaaca atgctttggt tcactaatga
    17161 aaatgcgtag cttgaaccta ctgcaccatt acctattaat acaactttgt tccctttaaa
    17221 tttgttcatt acaaaaactc ccttatgatt aattcactaa catacgtgta gtttcaaata
    17281 tgttagttta atgctgctta ttgacgatac aaaaacaaat aaacatctct tttattttca
    17341 acgcataact taaaaggtca tgtgtcatcc gcttttaagt ttgtgattta tttcacatat
    17401 aaaatgtaac atgcattaag tactaggtca atattaaatt gtgatttatt tcacatttta
    17461 ttttaatttt tacacctttt taatttgtat acgattacat cttagatgtc tttagtcttc
    17521 gtacttcgcc agtgattatt tacactttca catttttatt atcatgttta cttttttcta
    17581 agaaaacaac aatgtttttt gaattagtca aataaatgcg cgcaatcgtc ggtgtgcaaa
    17641 cagacaattg tacacaatgc ttattgataa gtatttaaaa aattaaaaat gtcatacaat
    17701 tatcaaattt gccattttat ttatattttc tcaaactaat taattgaata tcgaaatttt
    17761 tagtagaata atcaaaatag acagattaaa ggaggagtat catgcttaca gaacaagaga
    17821 aagacattat caaacaaaca gtgcctttac ttaaagagaa agggacagaa attacgtcaa
    17881 tcttttatcc aaaaatgttt aaagcgcatc ctgaactttt aaacatgttt aatcaaacga
    17941 accaaaaacg aggcatgcaa tcttcagcat tagcacaagc tgtaatggcc gcagcggtta
    18001 atatcgataa cttaagtgtt attaaaccag tcattatgcc agtcgcatat aaacactgcg
    18061 cactacaagt ttatgctgaa cattatccaa ttgtggggga aaatttatta aaagccattc
    18121 aagacgtgac aggattagaa gaaaatgacc ctgtcattca agcttgggca aaagcatatg
    18181 gcgttattgc ggatgtgttc atccaaattg aaaaagaaat ttatgatcaa atgatgtgga
    18241 taggttttaa accattcaaa attaccaata ttaaacaaga atctgaagac attaaatcat
    18301 ttacagttga aactgaagaa tatgacttta gtgaatttac accaggccaa tacatcacag
    18361 ttgatgtttc tagtgaaaaa cttccatata gagctaaacg tcactattct atcgtatcag
    18421 gtgaaaaaaa ccatttaact tttggcgtta aacgtgatgt cacaacagaa catgaaggcg
    18481 aagtttcaac aattttacac gatgagatta aagaaggcga tatgattaat ttagctgcgc
    18541 ctgtaggtgg attcgtatta gagaatacga ctgaaccaca acttttctta ggttcaggta
    18601 ttggtgttac acctttagta gctatgtatg aagctgcctc tgccaaaggt ttagatacac
    18661 agatggttca agttgctgaa aatgaacaac atttaccttt caaagacaac ttcaacagta
    18721 tcgcaagcca tcatgacaac gctaaattat atacacactt aaaagataaa caaggctata
    18781 ttggcgctga agaattacaa gcatttttag caaataaacc tgaaatttat atctgtggtg
    18841 gtacaaaatt cttacaatct atgatcgaag cacttaaatc tttaaattac gatatggatc
    18901 gcgtacacta cgaaacattt attccaagac taagcgttgc agtataactg agaaaagagg
    18961 tttagtgctc atatgaaact taatcaacgt tacgtaaaag tatttgcatt atatttcgta
    19021 agtattgtta ctgcaaatat tattgttaaa aataataatt taattaaaac attgatacaa
    19081 accatagccg ggtacacggt ctttgcagtt ggtttaaagt atttaactaa acgtaaaaat
    19141 aaataacata tatacaaaaa cagctatgac tttactttgt catggcagtt ttttaattgg
    19201 gagtgggaca gtaatgagat tttcgcaaaa tttatttcgt cgtcccacca caacatgcat
    19261 tgatgtatgc tcactgaatt tcataagaaa ggagttcaca agatgaccgt agatattgga
    19321 cggatttatg acaataaaga taataccgac gctattcgta tcctagtcga tagagtctgg
    19381 ccgagaggta tttcgaaaag aactgctaac ctagattatt ggttaaaaga cattgcccct
    19441 tctactgagt tgcgacaatg gttccaacat gatcctaaac tttttggagc ttttaaagaa
    19501 aaatatgaaa aagaattacg tgatcaggat gcacaaaaag atgcttttga aaaattaaag
    19561 gatattgtaa atcagcataa tcatgttcta ttgttatatg cagcaaaaga tactaaacat
    19621 aaccaagctg tagtactaca gcagttgctc aatacttagg tatatatgtt tatcgtgaat
    19681 cactataaat atcgctaact tcatttctaa tatttatttt taaaaagcat aattactaca
    19741 attaattgaa ctttaatgat tactaaactt gaacaatatt ttactttaac aaaataaagt
    19801 tttaaaatat taattgttgg gttttacata gtaattagaa agagagtgtt aatgaaatga
    19861 agtttaaaag actagcaact atattttcag cagtattagt gttatcagga tgtggctcaa
    19921 tgcattcatc tggcaaagac ttaaatattt cgttaccgtt aaaaacaaag tctattgcac
    19981 cttatgaaac agatgtacca gttaagattg gtgctgcaga atcattattt aaaacaaatg
    20041 atcaaggaaa aatagaaaaa gcattagtga aatcgtatca tcaaccaaat gatacaacgt
    20101 tagatataga attaaaagat aatattaaat ttcaaaacgg tcaaaaattg actgcagaaa
    20161 aagtgaaatc tagccttgaa aatagcatga aaaaaagcga cttggtcaaa tattcattac
    20221 caatatcatc aattaccgct aaaggtcaaa aactgacaat taaaaccaac tccgcttacc
    20281 ctgaacttgt atctgaatta gctaatcctt ttatggcaat ttatgataca gatgctaaat
    20341 cagatgttaa tcaaactcct gttggtacag gcccttacca aataaaagat tataagcaat
    20401 ctcgaaaaat atcattgtcg aattttaagg actattggca aggtaaaccc aaacttgatc
    20461 atattactgt gacgtaccaa gaagacggca ataatcgcgt cagaaattta gaatctcaaa
    20521 aagatgattt aataactgat gtcccagtta ataaagttca agacatagaa aataatcaaa
    20581 atttaaaagt gtcaaaagaa tctggattta gaacttcttt acttatgtat aatcatacta
    20641 ataaaaaaat gactaaatcc gttcgtgaag cattagatca tatcattgat agacaaggta
    20701 ttgcagatca tatttatcaa ggttatgcga aacctgcaac gagtccattt aatgataaaa
    20761 ttccatatat taaagaacct aagttaacca aacaaaatat cgaacaagca aaaacgttat
    20821 tagctaaaga tggttataca aaagaacacc ctttaaaaat taagttaatt acgtatgacg
    20881 gtcgtccaga gctatcgaaa attgcgcaag tattacaatc agatgctaaa aaagcaaata
    20941 ttgaaatcga cattaaaagt gttgatgaca tagaaggtta cttaaaagac cgttctgcat
    21001 gggatgcaac gatgtatagt tttgggacaa tccctcgcgg tgatacaggt tatttcttta
    21061 atcaagcata taaaaaagat ggtgctatta ataaaggaga ctacaataat agcaacgtcg
    21121 atgatttaat taatcaattg aatcatactg tagatgttaa ggaacgtcac aatatttcta
    21181 atgatattat taaattatca agtcgagatg taccaaacag ttatattgct tacaacgatc
    21241 aaatagtcgc tgctaacgct aaggttaaaa attataaagt aacacctgaa ggtatctatt
    21301 taatcgatta tcgaacaacg attgaacgat aagggagtgt gggacattga tttatgtctc
    21361 agccctgctt caactataaa aaccacgccg aagtcatcca atatgattat ggcgtggttt
    21421 aatttgctta tatgattgta ttaatttctg tttgatgaat ttcaataaaa tgattattac
    21481 atactttcag tgaatcatgt tcattttata taaatctaat tgtcactatt tgtttaacgt
    21541 gaaacacttt tttaactccg aaaagtattc ctattccact cttttaaaga caaattataa
    21601 aaatagctta tgtcacaaac atatgagcaa cataagcttt aattattatt cgtctacttt
    21661 tttctgttgt tctttaaatt ctttctgcag taaattcact tgttttaaac ctgcccccat
    21721 taaaatgaat acaagtatcc acacaataac gattaaaata aatattttta acgtatcact
    21781 gccaagtaca gttaaagatg tatcccaaat gccatgtaaa ccaacggctg ataaaaagaa
    21841 tattaaaaag cgtttatcaa aaatatcttt gaattcaaag ccatgctgtt ctttcgcaat
    21901 aactattgca gcaccaacaa tcgctgacca aactaaatga ccaccaatcg cagtccacgc
    21961 acgtgtgaag acaatatcta ataatgggac attttcccct aaagcgaaat tcaaaatata
    22021 acctgctgat tcaaaaactg cgaaccctgc accaatagca gcaccgatta ataatccatt
    22081 caaaatctta tttgttttca atttattgac gaaataaaca ataatgagtg cttttccagt
    22141 ttcttcaact aatcctacta aaaatgcatc gaaaaatgtt aaagaaccaa acctttcgaa
    22201 ttgatcacta aaaacgacaa atctatataa taacatcgta cttagtaatg aaaatacgcc
    22261 gccaataaag aacatgataa taacttcaaa aatgctaata tttttaaacg catttgattc
    22321 ataaaagaag aacaaacccg ataatggtac tgttaaagcc cctataaaaa tgagacccgg
    22381 aatcgcgtta gtgttattaa aaatcgctgc catgacccat aatccaataa atgttactgt
    22441 gaaagcgatg aatactcgag aaaagagcca tggcttcccc cattcttctg atatgtcaca
    22501 aattgctggc gtagttttag cagtacccgc tataaatatt tcatctgctt cgtttttagt
    22561 atgcggctta aatacttcag aaaacatatc ttttaaatta agatttaagc tttcatcatt
    22621 gccggctagt ctaccgattg atttggttgc attatcaaat aaacttcttg cttcggtttt
    22681 taaatctgca ttttgcgctg tttcatgcgc tttggcttcc atttgtttat tgatagattc
    22741 attatgtatg tcggacgtct tagttgattg tgtacttgct gatgacatcc cctcttcacc
    22801 tgtattaggt gcaactgatt gcgtttgtgt tgtctcttct ttttctgtat ttcttcgatg
    22861 attaagacct tccatagact tacctacttt cacttttcat aataaaacta ttttaattta
    22921 atattgatgg attttccata ttttaatata aat
//
LOCUS       DBNENW010000026        22896 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNENW010000026 DBNENW010000000
VERSION     DBNENW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.026"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(91..1089)
                     /gene="sirC"
                     /locus_tag="AC1LW8_000825"
     CDS             complement(91..1089)
                     /gene="sirC"
                     /locus_tag="AC1LW8_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HIP4772680.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMSGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1086..2081)
                     /gene="sirB"
                     /locus_tag="AC1LW8_000826"
     CDS             complement(1086..2081)
                     /gene="sirB"
                     /locus_tag="AC1LW8_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HIP4772681.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIAIGLGQNIKLIKWMIIMLISMLAGISVAVAGAIVFVGLIVPNISKRLLPPNYK
                     YLIPFTTLAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2097..3089)
                     /gene="sirA"
                     /locus_tag="AC1LW8_000827"
     CDS             complement(2097..3089)
                     /gene="sirA"
                     /locus_tag="AC1LW8_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HIP4772682.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3320..4300
                     /gene="sbnA"
                     /locus_tag="AC1LW8_000828"
     CDS             3320..4300
                     /gene="sbnA"
                     /locus_tag="AC1LW8_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HIP4772683.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4297..5307
                     /gene="sbnB"
                     /locus_tag="AC1LW8_000829"
     CDS             4297..5307
                     /gene="sbnB"
                     /locus_tag="AC1LW8_000829"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HIP4772684.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRDMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5328..7082
                     /gene="sbnC"
                     /locus_tag="AC1LW8_000830"
     CDS             5328..7082
                     /gene="sbnC"
                     /locus_tag="AC1LW8_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HIP4772685.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSDITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7075..8331
                     /gene="sbnD"
                     /locus_tag="AC1LW8_000831"
     CDS             7075..8331
                     /gene="sbnD"
                     /locus_tag="AC1LW8_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HIP4772686.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8321..10057
                     /gene="sbnE"
                     /locus_tag="AC1LW8_000832"
     CDS             8321..10057
                     /gene="sbnE"
                     /locus_tag="AC1LW8_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HIP4772687.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSNLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            9966..11816
                     /gene="sbnF"
                     /locus_tag="AC1LW8_000833"
     CDS             9966..11816
                     /gene="sbnF"
                     /locus_tag="AC1LW8_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HIP4772688.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11791..12567
                     /gene="sbnG"
                     /locus_tag="AC1LW8_000834"
     CDS             11791..12567
                     /gene="sbnG"
                     /locus_tag="AC1LW8_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HIP4772689.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12567..13769
                     /gene="sbnH"
                     /locus_tag="AC1LW8_000835"
     CDS             12567..13769
                     /gene="sbnH"
                     /locus_tag="AC1LW8_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HIP4772690.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITLSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13773..14537
                     /gene="sbnI"
                     /locus_tag="AC1LW8_000836"
     CDS             13773..14537
                     /gene="sbnI"
                     /locus_tag="AC1LW8_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HIP4772691.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14733..15140
                     /locus_tag="AC1LW8_000837"
     CDS             14733..15140
                     /locus_tag="AC1LW8_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772692.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFFSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15190..15815
                     /locus_tag="AC1LW8_000838"
                     /pseudo
     CDS             15190..15815
                     /locus_tag="AC1LW8_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16027..16803
                     /locus_tag="AC1LW8_000839"
     CDS             16027..16803
                     /locus_tag="AC1LW8_000839"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019152 - acetoin dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045150 - acetoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HIP4772693.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            16947..17069
                     /locus_tag="AC1LW8_000840"
     CDS             16947..17069
                     /locus_tag="AC1LW8_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772694.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17139..18119
                     /locus_tag="AC1LW8_000841"
     CDS             17139..18119
                     /locus_tag="AC1LW8_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIP4772695.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18082..18774
                     /locus_tag="AC1LW8_000842"
     CDS             18082..18774
                     /locus_tag="AC1LW8_000842"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIP4772696.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TFMVVITGEGSR"
     gene            18984..20150
                     /locus_tag="AC1LW8_000843"
     CDS             18984..20150
                     /locus_tag="AC1LW8_000843"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP4772697.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGKVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVAQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20131..21369
                     /locus_tag="AC1LW8_000844"
     CDS             20131..21369
                     /locus_tag="AC1LW8_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIP4772698.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGLLLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21359..22789
                     /locus_tag="AC1LW8_000845"
     CDS             21359..22789
                     /locus_tag="AC1LW8_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIP4772699.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRLLRHKTI"
BASE COUNT         7618 a         3546 c         4446 g         7286 t
ORIGIN      
        1 aactatatgt ataaaaaaac ctcgcaactg ttagttaact gaatcattta gctaacttcg
       61 ttgtgaggtc attttgtttt aataatatcg ttataacttt ttcacggtta ataataagta
      121 tatgaagaat ggggcaccaa aagcagcaat aaatacacct gctggcactt ctttaggcaa
      181 gaataaggta cgcccaatta agtctgcaat aacaattgat atggcaccaa tcattgctga
      241 cattagtaac tttttagcat aacttccgcg aacgattgtt ttcgcgatat gtggtgcgat
      301 taaaccgaca aacccaatgt tacctactaa actgattgcc atagatacga gtatagtaga
      361 agtgattaat tggattagtt tcatacgttg tacatgtaag cctaagccaa tcgctacagg
      421 gtcatcaagt atagatattt tcatttttgg tataacaaga aataacaacg gcacaacagc
      481 taaaataacc atacccaaga tgattgtatc tttaaacgta gcaccgtaaa gacttccgac
      541 tagccatgta taagctttgg cagcagataa ttgcttcgtt gtaatgagta atccttggac
      601 aagcgcaata aacaacgttt gcatcgaaat accgatgatt atgagtgttg tcgggcgtat
      661 ttgtcctttc gtttgaaaca ctaatagtat catcattgca attgcgccac ctaatacagc
      721 gaatagtgga agtaaatgta tcgttaaatg gctgaaaaat gcaataaaga caacggcact
      781 taagctagca ccacctgtga taccgataat atcaggtgag gcaattggat tttttaatac
      841 attttgcaac attaaaccac tcattcctag tgcggcacct gctaaaatcg caagtgtaat
      901 gcgaggtaag cgtaatactt ctaaagtgaa ttgatccata ctgtcatttg gatttataaa
      961 gtacatcagt acgcgttgta atggtataaa gcttgaacca ctcatcatac ttaccactga
     1021 aacgatggct aaaaagatta acgcgaagat gagatggtaa ttgtcttttt tattaatctt
     1081 ttcggtcata agcgttgacg tcctttcttc ataatataga ttaagacaat agcgccaatg
     1141 acagcggtaa cgacaccgat aggcaactct agtggcttaa ttattatacg agcaacaatg
     1201 tctgaaatga tcattaggat tgctccagct aatgtagtaa aaggaattaa atacttatag
     1261 tttggtggta ataatcgttt gctaatattc ggtacgataa gacccacaaa gacgattgct
     1321 ccagctacgg ctaccgaaat accggctaac atactgatga gcataataat catccatttg
     1381 attaatttta tgttttgacc gaggccgatt gcaatgtcgt cacttgtcat caagatgttg
     1441 atgtgtgcag ccatgctaaa tgcaattaaa ataagtatca atacaagcgg aataatccat
     1501 gggatatccc aaatattacg taatgaaacg gagccactta accaaaataa taggccttgt
     1561 aagtctgttt cgttcataat aagtatgcct tgagtaaagg ctgtaaatag catcgcaatc
     1621 gcagcacctg ccaaaatgac acggtgaggt gagaatagtg tttgtctaaa catacctagc
     1681 gcaacaacta atacagtaac aacaatggca cctaaaaatg caataactac aatcatttta
     1741 aaagattgaa tttggataaa tgtaatacta aaaatgacaa aaaatactgc gcctgcattg
     1801 acaccgaaaa gccctggtga tgctattggg tttcgtgtaa gtgcttgcat caacaaacct
     1861 gagacagcaa gggcagcacc agtcaataac gcaatgattg ttctcgacgc ccgtgcacca
     1921 gtgacaacat catgtaaatc gttttcacta tcaaagttga ataacgcctg tatcaccgta
     1981 cctggtgaca caagcgtatt tccaatcatt aaacttaaga tagctactat tgcaagacat
     2041 aaaccagcaa taacgatttg gtattttggt ttaagtagca tcgtaaaact ccttaattat
     2101 tttgattgtt tttcaatatt taacttttca tataaatcgt caatcaattt taatgatgat
     2161 ttatatccgc cagctaagtt ccaagtgatt tcatctaaat catcagatac ttggttgttt
     2221 ttaactgcgt ctaaattttt ccattcttta cttgaagtcc attcgctttc agtcttttta
     2281 actaatgcag catctttcgc atttggatct gattttacta caaaaatatg atcagcgttc
     2341 attaatggaa tgctttcttt ggatgtaagt tggataatat ctttaccatt atcaacttgt
     2401 ttttgtaagt ctttattacg tttgaatcct aaatcattta agatttcacc agcatatcca
     2461 ccagcataaa ttcttgtatg atcagcacgg aagttaacaa ctgaagcttt caatggccat
     2521 gcatctttat actttgcttt tgcatctttt tggaatgcag ctactttatc atcgtacttt
     2581 ttaagtaaat cttcagcttc tttttctttc cctaaagctt tccccattaa cttagttgta
     2641 tctttgaatt tgaaaactgt atcagtagaa actgttggtg cgattttaga taattgatcg
     2701 taaacttttt catttctaac ttttgacgcg acaattaagt ccggttttaa tttagagatt
     2761 tcctctaagt taggtgcagg ttcttgacct acaatcttag tatcttttaa atcatttttt
     2821 atgtattcga atttcggttt ttgtgtccat gattctacag cacctacagg tttaacacct
     2881 aaagatacag cgacgtcagt ggcaccttga tatagcgtaa caacacgctt tggtttccct
     2941 ttaatttcag ttgtacccat tgcatgttta attgaagttg tttccttatc tttgttatca
     3001 gatgattgtt tatttgaatt cccactacat cctgctaaaa caagtaggaa agcaagcgta
     3061 acaacaagca ttttaattac tttattcatt gactaattag cctccttcgt gatgtatgac
     3121 aatgagaatc attatcacta tttagtatga attaaatttt ttcctaagtc aataaaatat
     3181 ttatgattta catgcaactt ataattattt gacatataaa tgcataaaaa atataatcct
     3241 aattacttga tagtgagaat cattatcaat taggtaacac acaatattat agaattttaa
     3301 atttgaggag gaagcgcttt tgattgaaaa aagtcaagca tgtcacgatt cattgttaga
     3361 ttctgtaggg caaacaccta tggttcaact tcatcaacta tttccgaaac atgaagtgtt
     3421 tgcaaagtta gagtatatga atcctggagg cagcatgaaa gatcgacctg ccaagtacat
     3481 cattgaacat ggtattaaac atggtttaat cactgagaat acacatttaa ttgaaagtac
     3541 ttctggtaat ttaggcattg cgttggcaat gatagctaaa atcaagggat taaaactcac
     3601 gtgtgttgtt gatcctaaaa tatcaccaac aaatttgaaa attattaaaa gttatggtgc
     3661 caatgtagaa atggttgaag aacctgatgc acatgggggt tatttaatga ctcgtattgc
     3721 aaaggtgcaa gaactgttag ccactattga cgatgcatat tggattaatc aatatgcgaa
     3781 tgagttaaat tggcaatccc attatcatgg tgcaggcaca gagattgttg aaacaattaa
     3841 gcaacctata gattattttg tcgcgccagt cagcacgaca ggtagcatta tgggtatgag
     3901 tagaaaaata aaagaagtgc atccaaacgc acaaattgtt gctgttgatg cgaaagggtc
     3961 agtcattttt ggtgacaaac ctattaatag agaattacct ggtatcggtg ctagtcgtgt
     4021 acccgaaata ttgaatagat cagaaattaa tcaagtgatc catgtagatg attatcaatc
     4081 tgctttgggc tgtcgaaaac tgattgatta tgaaggcata tttgccggag gttcaacagg
     4141 ttcgattatt gcagcgattg agcagttgat aacgtcaatt gaagaaggtg caacaattgt
     4201 cacgatttta ccagatcgag gcgatcgtta cttagattta gtttattcag atacatggtt
     4261 agaaaaaatg aaatcaagac aaggagttaa atcagaatga atagagagat gttgtattta
     4321 aatagatcag atattgaaca agcgggaggt aatcattcac aagtttatgt ggacgcatta
     4381 acagaagcat taacagccca tgcgcacaat gattttgtac aaccgcttaa gccgtattta
     4441 agacaggatc ctgaaaatgg acacatcgca gatcgaataa ttgcaatgcc aagtcatatc
     4501 ggtggtgaac acgcaatttc aggtattaag tggataggta gtaagcacga caatccatcg
     4561 aaacgtgata tggagcgtgc aagtggtgtc attattttga atgatccaga aacgaattat
     4621 ccaattgcag ttatggaagc aagtttaatt agtagtatgc gtactgcagc agtttcagtg
     4681 attgcagcta agcatttggc taaaaaagga tttaaagact taacaatcat tggatgcggg
     4741 ctaatcggag acaagcaatt acaaagtatg ttagagcaat tcgatcatat tgaacgcgtg
     4801 tttgtttacg atcaattctc tgaagcatgt gcacgctttg ttgatagatg gcaacaacag
     4861 cgtccggaaa ttaattttat tgcgacagaa aatgctaaag aagcagtatc aaatggtgaa
     4921 gtagtcatta catgtaccgt aacggatcaa ccatacattg aatatgattg gttacaaaag
     4981 ggtgcattta ttagcaacat ttctatcatg gatgtgcata aagaagtctt tattaaagct
     5041 gacaaagtcg tagtagacga ctggtcacaa tgtaatcgag aaaagaaaac tattaaccaa
     5101 ttggtgttag aaggtaaatt cagcaaagaa gcacttcatg ctgaactagg acaacttgtg
     5161 acaggtgaca taccaggacg tgaagacgat gatgaaatca tattacttaa tccgatgggt
     5221 atggctatcg aagatatttc aagtgcttac tttatttatc aacaggcaca acaacaaaat
     5281 attgggacaa cattgaacct atattaagaa tgcgaggtgt ctgaacattg cagaatcata
     5341 cagcagtcaa tacagcacaa acgataatat taagagattt agttgatgca ttattatttg
     5401 aagatatagc cggtattgta tcgaatagtg atattactaa agaaaatgga caaacgcttt
     5461 tgatatacga acgtgaaaca caacaaataa agatacctgt ttattttagt gctttaaata
     5521 tgtttcgtta tgaaagctca caaccaatta cgatagaggg aagggcgtct aagcaacctt
     5581 taacagcagc tgaattttgg caaacaattg ctaatatgaa ttgtgattta agtcatgaat
     5641 gggaagtggc tcgagttgaa gaaggactga ctactgctgc cacacagctt gctaaacaat
     5701 tatcagaatt agatttagcg tcacatcctt ttgtgatgtc agagcagttt gcaagtttaa
     5761 aagatcgtcc atttcatcca ttagctaaag aaaaaagagg attaagagaa gcggattatc
     5821 aagtgtatca agctgaatta aatcaatcat ttcctttaat ggttgcagca gttaaaaaga
     5881 cacatatgat tcatggcgat actgcaaata tcgatgaatt agaaaatttg acagcaccta
     5941 taaaagaaca agcgacagac atgttaaatg atcaagggtt atcaatagat gactatgtac
     6001 tatttccggt acatccttgg caatatcagc atattttgcc gaacgtcttt gcgaaagaga
     6061 ttagtgaaaa gttggttgta ctattaccgt taaaatttgg agattatctg tcgtcttcaa
     6121 gtatgcgttc attaattgat attggcgcac cgtataacca tgtcaaagta ccatttgcaa
     6181 tgcagtcatt aggggcatta aggctaacgc ctacgcgtta catgaaaaac ggagaacaag
     6241 cagaacaatt attacgtcag cttatagaaa aagatgaagc actagctaag tatgtcatgg
     6301 tttgtgatga aacagcttgg tggtcatata tgggtcaaga taatgatatt ttcaaagatc
     6361 aattaggtca tctaactgtt cagctaagaa aatatcccga agtgctagcc aaaaatgata
     6421 cgcaacagct agtgtcaatg gcagcactcg cggcaaatga tcgcacttta tatcaaatga
     6481 tttgtggaaa agataatatt tctaaaaatg atgtcatgac gttatttgaa gatatcgcgc
     6541 aagtcttttt aaaggtaaca ctatcattta tgcaatacgg cgcattacca gagttgcatg
     6601 gtcaaaatat attgttgtca tttgaagatg gacgtgtaca aaaatgcgtg ttacgtgatc
     6661 atgatactgt cagaatttat aaaccatggc taacagcaca tcagctttca ttgccgaagt
     6721 atgtcgtcag agaagataca cctaatacgc taattaatga agatttggaa acattctttg
     6781 cttattttca aacattagct gtatcggtaa atctatatgc cattattgat gcaattcaag
     6841 atttatttgg tgtaagtgag catgaactta tgtcgttgtt aaaacaaatt ttaaagaatg
     6901 aagtggcaac tatttcctgg gttacaactg atcagctagc tgtcagacac attttatttg
     6961 ataaacagac gtggccattc aaacaaattt tattaccatt gctatatcaa cgtgatagtg
     7021 gtggaggtag tatgccttca ggtttaacta ccgtaccaaa tccaatggtg acatatgatt
     7081 aatcagtcta tatggcgcag taactttcgc attttatggc tcagtcagtt tatagcgatt
     7141 gctggactga cagtacttgt gccattattg ccaatttata tggcatcact acaaaatcta
     7201 tcagtcgtag aaatacagtt gtggagtggt atagcgattg ctgctccagc tgtaacgacg
     7261 atgatagctt cgccgatatg ggggaagcta ggtgataaga ttagccggaa atggatggtg
     7321 ttaagagcgt tacttggttt ggcggtatgc ttatttttaa tggcattgtg tacgacacca
     7381 ttacagtttg tacttgtgag gttattgcag ggactatttg gtggtgttgt tgatgcatca
     7441 agtgcgtttg cgagtgcaga ggcgccagct gaagatcgtg gaaaggtatt aggaagactg
     7501 caaagttcag tcagcgcagg gtctcttgtg gggccattaa ttggcggtgt tacagcttcg
     7561 atattaggtt ttagtgcgtt actgatgagt attgccgtta ttacttttat tgtctgtatt
     7621 ttcggtgcat taaaattgat tgaaacgaca catatgccaa aatcacaaac accaaatatt
     7681 aataaaggta ttcgccgttc atttcaatgt ctattatgca cacaacaaac atgtcgattt
     7741 attatcgttg gcgttttagc aaactttgct atgtatggca tgctaactgc attatcacca
     7801 cttgcttcat cagtgaatca tacagcgata gatgaccgta gtgtgattgg atttttacag
     7861 tccgcatttt ggacggcttc gatattaagc gcgcctttat ggggacgctt taatgataaa
     7921 tcatatgtta aatcagtata tatatttgcc acgattgcat gtggttgtag tgcgatactg
     7981 caaggtttag cgacgaatat agagttttta atggctgcaa gaatacttca aggattaaca
     8041 tatagtgcat tgattcaaag tgtcatgttt gttgtcgtga atgcgtgtca tcaacaactt
     8101 aaaggcacat ttgttggaac gacgaacagt atgttagttg ttggtcaaat tattggcagt
     8161 cttagtggcg ctgccattac aagttatact acaccagcta ctacgtttat cgttatgggc
     8221 gtagtatttg cagtaagtag tttattttta atttgttcaa ccatcactaa tcaaatcaac
     8281 gatcacacat taatgaaatt atgggagttg aaacaaaaaa gtgcaaaata aagaattaat
     8341 acaacatgca gcgtatgcgg ctatcgaacg cattttaaat gaatatttta gagaagaaaa
     8401 tttatatcaa gtaccacctc aaaatcatca atggtctata caattatcag agctcgaaac
     8461 tttaacgggt gaatttcgct attggtctgc gatggggcat catatgtatc atccagaggt
     8521 atggcttatc gatggaaaaa gtaaaaaaat aacaacttat aaagaagcaa ttgcgcgtat
     8581 tttgcaacat atggctcaaa gtgcagataa tcaaacggca gtgcaacaac atatggcgca
     8641 aattatgtct gacatcgata atagcattca tcgcacggcg cgttatttgc aaagtaacac
     8701 aatagactac gtagaggatc gttatatcgt ttcagaacaa tctttatact taggtcatcc
     8761 atttcatccg actcctaaga gtgcaagtgg gttttcagaa gcagatttag agaaatatgc
     8821 acccgaatgt catacatcat tccaattgca ttatttagct gtgcatcaag atgttctgct
     8881 cacgcgctat gtagaaggta aagaagatca ggttgagaaa gtgttgtatc aattagcaga
     8941 catagatata tcagagatac ccaaagattt tattttatta ccaacacatc cttatcaaat
     9001 caatgtgttg cgacagcatc cacagtatat gcaatatagt gaacaaggtt taataaaaga
     9061 ccttggcgtt tccggtgatt cagtgtaccc gacgtcttcg gttagaactg tattttcaaa
     9121 agcattaaac atttatttaa aattaccgat acacgttaaa atcactaatt ttatacgtac
     9181 gaatgacctt gaacagattg aacggacaat tgatgccgcg caagttatcg catcagtcaa
     9241 agatgaggtt gaaacacccc attttaaatt gatgtttgaa gaaggatatc gtgcattgtt
     9301 accgaatcca ttagggcaaa cagttgaacc tgaaatggat ttattaacaa atagtgccat
     9361 gattgttcgt gaagggatac cgaattacca tgctgataaa gatattcatg tattggcgtc
     9421 attatttgaa acgatgcctg attcaccgat gtctaattta tcacaagtga ttgagcaaag
     9481 tggtttagca ccagaagcat ggcttgaatg ttatttgaat cgtacattat tgccgatatt
     9541 aaagctgttt agtaacacag gcattagtct agaagcacat gtacaaaata cattaattga
     9601 attaaaagat ggcatacccg acgtatgctt tgtcagagat cttgaaggca tttgtctatc
     9661 tagaacgatt gctactgaaa aacaacttgt gccaaatgtt gtggcagcat caagccctgt
     9721 tgtatatgca catgatgaag catggcatcg tcttaaatat tacgttgtag taaatcactt
     9781 aggacattta gtatcaacta ttggtaaagc gactagaaat gaagttgtgt tatggcaact
     9841 tgtagcgcat cgtcttatga cttggaaaaa agaatacgcg aataacgcag tgtttgttga
     9901 ctgtgtagaa gatttatatc aaacgccgac cattgcggct aaagcgaatt tgatgagtaa
     9961 attgaatgat tgtggtgcaa accctattta tacacatata ccaaatccaa tttgtcataa
    10021 caaggaggta tcgtattgtg aatcaaacaa ttcttaatcg tgtaaagact agagtgatgc
    10081 accaactggt atcatcactt atttatgaga atattgttgt gtataaagcg tcatatcaag
    10141 acggtgtcgg tcattttaca atagaaggac atgattcaga gtatcgtttt actgctgaaa
    10201 agacacatag ctttgatcgt atacgtatca catcaccaat tgagcgtgtc gtaggagatg
    10261 aggcagatac aacaacagac tatacacaat tattgagaga ggttgtattt acatttccta
    10321 aaaatgatga aaagctagaa caatttattg ttgagttatt acagacagaa ttaaaagata
    10381 cacaaagtat gcagtatcga gaatcaaacc caccagcaac acctgagaca tttaacgact
    10441 atgaatttta tgcgatggaa gggcatcagt atcatccaag ttacaaatca cgtttaggat
    10501 ttacgttgag tgataatttg aaatttggtc ctgattttgt accaaacgtt aaactgcagt
    10561 ggttagctat cgacaaagat aaagtagaaa cgacggtatc aagaaatgtt gtagttaacg
    10621 aaatgttacg tcaacaagtt ggcgataaga cttatgaaca ttttgtacag caaattgaag
    10681 cgtctggcaa acatgtaaat gatgttgaga tgatacctgt acacccatgg cagtttgaac
    10741 atgtcatcca agttgatttg gctgaagaaa ggcttaatgg cacagtacta tggttagggg
    10801 aaagtgatga gctatatcat cctcaacaat cgattcgtac gatgtcgcca atagacacga
    10861 caaaatatta tttaaaggta ccaataagta taacgaacac ttcaacgaaa cgagtgttgg
    10921 cgcctcatac aattgaaaat gcagcgcaaa ttacggattg gttaaagcag atacagcaac
    10981 aagatacgta tttaaaagat gaattaaaga cagcttttct aggggaagtc ttaggacagt
    11041 cttatttaaa tacacaactt tcgccttata aacaaactca agtttatggt gcgttaggtg
    11101 ttatatggcg tgaaaatata tatcacatgt taatcgatga agaggatgcg ataccattta
    11161 atgcacttta tgcaagtgat aaggatggtg taccattcat tgaaaattgg attaaacaat
    11221 atggttctga agcttggaca aagcaatttt tagctgtagc gattcgtcca atgattcata
    11281 tgctttatta tcacggtatt gcctttgaat cgcatgcaca aaatatgatg ctcattcatg
    11341 aaaatggttg gcctacacgt attgccttaa aagatttcca tgatggtgtt cgttttaagc
    11401 gtgagcattt aagtgaagct gcttcacacc tgacattaaa gccaatgcca gaagcacata
    11461 aaaaagtgaa tagtaattca tttattgaaa cagatgacga acgtttagta cgcgactttt
    11521 tacatgatgc atttttcttt attaatatcg ccgaaatcat cttatttatt gaaaagcaat
    11581 atggtatcga tgagcagcga caatggcaat gggttaaaga cattatcgag gcgtatcaag
    11641 aagcatttcc agagttgaat aactatcaac atttcgattt gtttgaacct acgattcaag
    11701 ttgaaaagtt aacgacacgt cgattattaa gtgactccga gttaagaatt catcatgtta
    11761 caaatccatt aggtgtagga ggtatcaatg atgcaacaac tatctctgaa acatagatta
    11821 aacaatggtg attcagttta tggcattttt aattctatac cggacccatt gatgatcgag
    11881 gttatcgcag caagcgggta tgactttgtt gtgattgata cagaacacgt ggcgattaat
    11941 gatgagacac tagcgcattt aattcgtgca gctgaagcag cgcatattat accaattgta
    12001 cgtgtcactg cagtgataga tagagatatc attaaagtgt tagatatggg tgcgagaggt
    12061 attattgtgc cacacgttaa agatcgtgag acagttgagc atattgtgaa attaagtcgt
    12121 tattacccgc aaggattaag aagtttgaat ggtggtcgca tggcaagatt tggacgtaca
    12181 ccattacttg atgcaatgga gatggctaat gagcatatta tggtgattgc catgatagaa
    12241 gatgttgaag gggttatggc cattgacgat atagcacaag tcgaaggttt agacatgata
    12301 gtcgaaggtg ccgcagattt atcgcagtca cttggcatac catggcaaac gcgtgatgat
    12361 caagtaacat cacatgttca acatattttt gaggttgtga atgcacatgg taaacatttt
    12421 tgtgcattac cacgtgaaga tgaagatatt gcaaaatggc aggcacaagg tgtacaaaca
    12481 tttattttag gtgatgatcg cggaaaaata tatcgccatt taagtgcatc tctagcgacg
    12541 tctaaacaga aaggggatga tggctaatgc gtatagttca acctgttatt gaacaattaa
    12601 aagcacaatc tcatccagtt tgtcattata tctatgattt agtcggactg gaacatcatt
    12661 tgcaacatat tacattgtca ttgccgagta attgtcaaat gtactatgca atgaaagcaa
    12721 atagtgaacg aacaatccta gatacaatta gtcagtatgt tgaaggattc gaagttgcat
    12781 ctcaaggtga aatagcaaaa ggtcttgctt ttaaaccagc aaatcatatt atttttggag
    12841 gccctggtaa gacagacgag gaactaagat atgcagtaag tgaaggtgtt cagcgtattc
    12901 atgttgaaag tatgcatgaa ttacaacggc taaatgccat cttagaagat gaagataaga
    12961 cacaacacat tttattgcgt gttaatttag caggaccatt tcccaatgca acgttgcata
    13021 tggcaggacg cccaacacaa tttggtattt ctgaagacga agttgatgat gtcattgaag
    13081 ctgcgctagc aatgccaaat attcatctag atggctttca ttttcattct atttctaaca
    13141 atttagactc gaatttacac gtcgatgtag tgaaacttta ttttaaaaaa gcaaaatcat
    13201 ggtctgaaaa acatcgattt ccactcaaac atatcaatct tggtggtggt ataggggtta
    13261 actatgcaga tttaactagc caatttgagt gggataattt tgtagaaaat tttaaaacac
    13321 ttatcgttga gcaagaaatg gaagatgtga cattgaactt tgaatgtggg cgctttattg
    13381 tggcacatat tggttactat gtgacagaag tgctagatat taagaaagtg catggtgctt
    13441 ggtatgccat tttaagagga ggtacgcaac aatttagact gccggtatct tggcagcata
    13501 accatccttt tgaaatttat cgctataagg acaatccata ttcatttgaa aaagtttcaa
    13561 tttcgagaca ggacacaacg ttagtcggtc aattatgtac accgaaagat gtctttgcta
    13621 gagaagtaca gatagacgca atcagtacag gcgacgttat tgttttcaaa tatgcaggtg
    13681 catacggatg gtctatttca catcacgatt tcttaagcca tccacatcct gaatttattt
    13741 atttaacgca aacaaaggag gatgaataac tattgaatca tattcatgaa catttaaaat
    13801 tggtaccagt agataagatt gatcttcacg aaacattcga acctttaaga ttggaaaaga
    13861 cgaaaagtag tattgaagca gatgatttta tacgtcatcc tattttagtg acagcgatgc
    13921 aacatggtag atatatggtt atagatggtg tgcatcggta tacaagtttg aaagcgttag
    13981 gatgtaagaa agttccagtg caagaaatcc atgaaacaca atattcaatt agtacgtggc
    14041 aacataaagt tccatttggt gtgtggtggg aaacgttaca acaagaacat cgcttgccat
    14101 ggactactga gacaagacaa gaagcgccat ttattacgat gtgtcatggt gatacagaac
    14161 aatatttgta tacgaaagat ttaggcgaag cacattttca agtatgggaa aaggttgtcg
    14221 caagttatag tggttgttgt tctgtagaga gaattgcaca aggtacatat ccttgtcttt
    14281 ctcaacaaga tgtactcatg aagtatcagc cattgagtta taaggaaatt gaagcggttg
    14341 ttcataaagg ggaaactgtg ccagcaggtg tgacacgctt taatatttca ggacgatgtc
    14401 ttaatcttca agtaccactg gcattactta aacaagatga tgatgttgaa caactgcgca
    14461 attggaagca gtttttagca gataagtttg ccaatatgag atgctatact gaaaaagtat
    14521 acttggtgga gcaatagttt tactgtgatg ttgagggaaa tatgatgatt tagcgtattg
    14581 atagcgaaaa tataataaaa caatatagtg tggagaactt ttgatatttt ataaatattg
    14641 aagttctcca tttttgtatt ttgcatataa aaattaaata aaataaggta tattaaggta
    14701 aagtataaat tttaaataaa tggggaatga gtatgagctc aattatagga aaaatagcaa
    14761 tttggatagg catcgtagct caaatatatt ttagtgtcgt ttttgttagg atgatatcta
    14821 ttaatattgc tggaggatct gattacgaaa caattttttt attaggatta atattggctc
    14881 ttttcactgt tttaccaacc atctttactg cgatttatat ggaaagttac tctgtaatcg
    14941 gaggtgcact ttttattgtt tatgctatta ttgcactgtg tttatataat ttcttttcgt
    15001 caattttatg gctgattggt ggtattttgc tgatttggaa taaatactca aaagatgaat
    15061 cgacagacga aaatgaaaaa gttgatattg aaagtacaga gaatcaattt gaatctaaag
    15121 ataaaatcac taaagaataa agagaatatt taaggcggag tataaatatt aaataaatgg
    15181 ggaatagaca tggaaaaaaa tgtagaaaaa tcattcataa agataggttt atattttcaa
    15241 atagcttata tagtactcat ggctataact ttatgtgggt ttgtaatttg ctatggacta
    15301 attttcggcc ttttctattt attatcaggt agcacagctg attattcaat agtaacaata
    15361 gttatatcgg caataatttc tatatttgta attatacttt caatcgtacc tgtcatcgta
    15421 ttggcatctg acttatttaa agaaaggatt tcaaaaggtg tcatattaat tgtattggct
    15481 attatcgctt tagtattatg caactttgta tctgcaatac tctggtttgt ttcagccata
    15541 tctattttag gtagaaaaaa attagtatct acagcagata ctaccactat tcaaaaaagt
    15601 aaagggaacg caaatcaagc atcacataaa gacacgtgta aaaaggaact tgatagtcaa
    15661 gacatgatgg aacatcctga gtttaaaaat cccacgacta aaaacctaga aggattaaac
    15721 gaagaaatac ataaagatga agctacaact aagttgacag tgataacacg caaccgccga
    15781 ttgaatcaaa agaccatgtc tcgaaaaaag attgatgaca aactaatcga gagacttaaa
    15841 aaaataatat tcaacataag aacttttaaa acgacattta aacacattgc caatcactaa
    15901 tggtagtgcg tttaactata ccttaaatat ctgaatattt tgttaaatgg agctaccttt
    15961 gttgtactat tcaaatgaag aggagtaaaa tgtaattaaa ggaaagtaat ttgaggagtg
    16021 atctttatga caaacaacaa agtagcgtta gtaactggcg gagcacaagg gattggtttt
    16081 aaaattgcag aacgtttagt ggaagatggt ttcaaagtag cagttgttga tttcaatgaa
    16141 gaaggggcaa aagcagctgc acttaagtta tcaagtgatg gtacaaaagc tattgctatc
    16201 aaagcagatg tatcaaaccg tgatgatgta tttaatgcag taagacaaac tgcagctcaa
    16261 tttggcgatt tccatgtcat ggttaacaat gccggccttg gaccaacaac accaatcgat
    16321 acaattactg aagaacagtt taaaacagta tatggcgtga acgttgcagg tgtgctatgg
    16381 ggtattcaag ccgcacatga acaatttaaa aaattcaatc atggcggtaa aattatcaat
    16441 gcaacatctc aagcaggcgt tgagggtaac ccaggcttgt ctttatattg cagtacaaaa
    16501 ttcgcagtgc gaggtttaac acaagtagcc gcacaagatt tagcgtctga aggtattact
    16561 gtgaatgcat tcgcacctgg tatcgttcag acaccaatga tggaaagtat cgcagtggca
    16621 acagccgaag aagcaggtaa acctgaagca tggggttggg aacaatttac aagtcagatt
    16681 gctttgggca gagtttctca accagaagat gtttcaaatg tagtgagctt cttagctggt
    16741 aaagactctg attacattac tggacaaaca attattgtag atggtggtat gagattccgt
    16801 taataatcat gcattagcga taaataaatc cttattgtta agtttaatca cttagcagta
    16861 aggatttttt agtgcactta gaagggagtg tattggtaga aaattaataa gcgaagttct
    16921 taaatgagtt atgatgtcac tgtctaatgc atcagttgaa agcattacta gtattaacac
    16981 acccaagata ttataaaaca tcacaaaaac accactatct aatttatctc aataaaaatt
    17041 cacaaagtta tctcatttta tttttataaa taaaaaatat cgataaaaag cttacaatac
    17101 tttatgtttt tatgatatat ttttaatgta taaatgaggt ggaagatttg gaaagagttt
    17161 tgataactgg tggggctggt tttattgggt cgcatttagt agatgattta caacaagatt
    17221 atgatgttta tgttctagat aactatagaa caggtaaacg agaaaatatt aaaagtttgg
    17281 ctgacgatca tgtgtttgaa ttagatattc gtgaatatga tgcagttgaa caaatcatga
    17341 agacatatca atttgattat gttattcatt tagcagcatt agttagtgtt gctgagtcgg
    17401 ttgagaaacc tatcttatct caagaaataa acgtcgtagc aacattaaga ttgttagaaa
    17461 tcattaaaaa atataatagt catataaaac gttttatctt tgcttcgtca gcagctgttt
    17521 atggtgatct tcctgatttg cctaaaagtg atcaatcatt aatcttacca ttatcaccat
    17581 atgcaataga taaatattac ggcgaacgga cgacattaaa ttattgttcg ttatataaca
    17641 taccaacagc ggttgttaaa ttttttaatg tatttgggcc aagacaggat cctaagtcac
    17701 aatattcagg tgtgatttca aagatgttcg attcatttga gcataacaag ccatttacat
    17761 tttttggtga cggactgcaa actagagatt ttgtatatgt atatgatgtt gttcaatctg
    17821 tacgcttaat tatggaacac aaagatgcaa ttggacacgg ttataacatt ggtacaggca
    17881 cttttactaa tttattagag gtttatcgta ttattggtga attatatgga aaatcagtcg
    17941 agcatgaatt taaagaagca cgaaaaggag atattaagca ttcttatgca gatatttcta
    18001 acttaaaggc attaggattt gttcctaaat atacagtaga aacaggttta aaggattact
    18061 ttaattttga ggtagataat attgaagaag ttacagctaa agaagtggaa atgtcgtgaa
    18121 aatgacattg aagctgtcca taataataag ggttatgcct atcaaagaaa attagacaaa
    18181 ctagaagaag tgagaaaaag ctattaccca attaaacgtg cgattgactt aattttaagc
    18241 attgttttat tatttttaac tttaccgatt atggttatat tcgccattgc tatcgtcata
    18301 gattcgccag gaaaccctat ttatagtcag gttagagttg ggaagatggg taaattaatt
    18361 aaaatataca aattacgttc gatgtgcaaa aacgcagaga aaaacggtgc gcaatgggct
    18421 gataaagatg atgatcgtat aacaaatgtc gggaagttta ttcgtaaaac acgcattgat
    18481 gaattaccac aactaattaa tgttgttaaa ggggaaatga gttttattgg accacgcccg
    18541 gaacgtccgg aatttgtaga attatttagt tcagaagtga taggtttcga gcaaagatgt
    18601 cttgttacac cagggttaac aggacttgcg caaattcaag gtggatatga cttaacaccg
    18661 caacaaaaac tgaaatatga catgaaatat atacataaag gtagtttaat gatggaacta
    18721 tatatatcaa ttagaacatt tatggttgtt attacagggg aaggctcaag gtagtcttaa
    18781 tttacttaat aagttcaaat aaaagttata ttttaaagat tgtgaccaat tgttacagta
    18841 taacgaggaa tcccttgaga cagtatcaaa tggcattaag aaatatgtgc catcattgat
    18901 ttgcatggct ataaatacta ttcatctgat gagatagcca tgttaagaaa ttgaaagtat
    18961 agcattaaag gggtttgtaa cagttgaaaa ttatatattg tattactaaa gcagacaatg
    19021 gtggtgcaca aacacatctc attcaacttg ccaaccattt ttgcgtacac catgatgttt
    19081 atgtcattgt aggcaatcat ggaccaatga ttgaacaact agatgcaaga gttaatgtaa
    19141 ttattatcga acatttagta ggtccaattg actttaaaca agatatttta gctgtcaaag
    19201 tgttagcgca gttattctcg aaaattaaac ctgatgttat ccatttacat tcttccaaag
    19261 ctggaacggt cggacgaatt gcgaagttca tttcgaaatc gaaagacaca cgtatagttt
    19321 ttactgcaca tggatgggct tttacagagg gtgttaaacc agctaaaaaa tttctatatt
    19381 tagttatcga aaaattaatg tcacttatta cagatagcat tatttgtgtt tcagatttcg
    19441 ataaacagtt agcgttaaaa tatcgattta atcgattgaa attaaccaca atacataatg
    19501 gtattgcaga tgttcccgct gttaagcaaa cgctaaaaag ccaatcacat aacaatattg
    19561 gcaaagtagt tggaatgttg cctaataaac aagatttaca gattaatgcc ccgacaaagc
    19621 atcaatttgt tatgattgca agatttgctt atccaaaatt gccacaaaat ctaatcgcgg
    19681 caatagagat attgaaatta cataacagta atcatgcgca ttttacattt ataggcgatg
    19741 gacctacatt aaatgattgt cagcaacaag ttgcacaagc tgggttagaa aatgatgtca
    19801 catttttggg caatgtcatt aatgcgagtc atttattatc acaatacgat acgtttattt
    19861 taataagtaa gcatgaaggt ttgccaatta gcattataga agctatggct acaggtttgc
    19921 ctgttatagc cagtcatgtt ggcggtattt cagaattagt agctgataat ggtatatgta
    19981 tgatgaacaa tcaaccagaa actattgcca aagtcttgga aaaatattta atagacagtg
    20041 attacatcaa aatgagtaat caatctagaa aacgttattt agaatgtttt actgaggaga
    20101 aaatgattaa agaagtggaa gacgtttata atggaaaatc aacacaatag taaattacta
    20161 acattgttac ttatcggttt agcggttttt attcagcaat cttcggttat tgccggtgtg
    20221 aatgtttcta tagctgactt tatcacatta ctaatattag tttatttact gtttttcgct
    20281 aaccatttat taaaggcaaa tcatttttta cagtttttca ttattttgta tacatatcgt
    20341 atgattatta cgctttgttt gctatttttt gatgatttga tatttattac ggttaaggaa
    20401 gttcttgcat ctacagttaa atatgcattt gtagtcattt atttctattt agggatgatc
    20461 atctttaagt taggtaatag caaaaaagtg atcgttacct cttatattat aagcagtgtg
    20521 actataggtc tattttgtat tatagctggt ttgaacaagt cccctttact aatgaaattg
    20581 ttatattttg atgaaatacg ttcaaaagga ttaatgaatg accctaacta tttcgcgatg
    20641 acacagatta ttacattggt acttgcttac aagtatattc ataattacat attcaaggtc
    20701 cttgcatgtg gtcttttact atggtcttta actacaacgg ggtctaagac tgcgtttatc
    20761 atattaatcg tcttagccat ttatttcttt attaaaaagt tatttagcag aaatgcggta
    20821 agtgttgtga gtatgttagt gattatgctg atattacttt gttttacttt ttataatatc
    20881 aactactatt tattccaatt aagcgacctt gatgccttac cgtcattaga tcgaatggcg
    20941 tctatttttg aagaggggtt tgcatcatta aatgatagtg gatctgagcg aagtgttgta
    21001 tggataaatg ccatttcagt aattaaatat acattaggtt ttggtgttgg cttagtggat
    21061 tatgtacata ttggctcgca aattaatggt attttacttg ttgcccataa tacatatttg
    21121 caaatttttg cggaatgggg cattttattc ggtgcgttat ttatcatatt tatgctttat
    21181 ttactgtttg aattatttag atttaacatt tctgggaaaa atgtaacagc aattgttgta
    21241 atgttgacga tgctgattta ctttttaaca gtatcattta ataactcaag atatgtcgct
    21301 tttattttag gaattatcgt ctttattgtt caatatgaaa agatggaaag ggatcgtaat
    21361 gaagagtgat tcactaaaag aaaatattat ttatcaaggg ctataccaat tgattagaac
    21421 gatgacacca ctgattacaa tacccattat ttcacgtgca tttggtccca gtggtgtggg
    21481 tattgtttca ttttctttca atatcgtgca atactttttg atgattgcaa gtgttggcgt
    21541 tcagttatat tttaatagag ttatcgcgaa gtccgttaac gacaaacggc aattgtcaca
    21601 gcagttttgg gatatctttg tcagtaaatt atttttagcg ttaacagttt ttgcgatgta
    21661 tatggtcgta attactatat ttattgatga ttactatctt attttcctac tacaaggaat
    21721 ctatattata ggtgcagcac tcgatatttc atggttttat gctggaactg aaaagtttaa
    21781 aattcctagc ctcagtaata ttgttgcgtc tggtattgta ttaagtgtag ttgttatctt
    21841 tgtcaaagat caatcagatt tatcattgta tgtatttact attgctattg tgacggtatt
    21901 aaaccaatta cctttgttta tctatttaaa acgatacatt agctttgttt cggttaattg
    21961 gatacacgtc tggcaattgt ttcgttcgtc attagcatac ttattaccaa atggacagct
    22021 caacttatat actagtattt cttgcgttgt tcttggttta gtaggcacat accaacaagt
    22081 tggtatcttt tctaacgcat ttaatatttt aacggtcgca atcataatga ttaatacatt
    22141 tgatcttgta atgattccgc gtattaccaa aatgtctatc cagcaatcac atagtttaac
    22201 taaaacgtta gctaataata tgaatattca attgatatta acaataccta tggtctttgg
    22261 tttaattgca attatgccat cattttattt atggttcttt ggtgaggaat tcgcatcaac
    22321 tgtcccattg atgaccattt tagcgatact tgtattaatc attcctttaa atatgttgat
    22381 aagcaggcaa tatttattaa tagtgaataa aataaggtta tataatgcgt caattactat
    22441 tggtgcggta atgaatttag tattatgtct tgttttgata tatttttatg gaatttacgg
    22501 tgcggctatt gcacgtttaa ttacagagtt tatcttactt atttggcgat ttgttgatat
    22561 tactaaaatc aatgtgaagt tgaatattgt aagtacgatt caatgtgtca ttgctgccgt
    22621 tatgatgttt attgtgcttg gtgtggtcaa tcattatttg ccccctacaa tgtacgctac
    22681 gctgctatta attgcgattg gtatagtagt ttatctttta ttaatgatga ctatgaaaaa
    22741 tcaatacgta tggcaaatat tgaggcttct tcgacataaa acaatttaag tatcggtaac
    22801 gctatacttt agaaatttaa gattaagaag aaaaggcaat ttcttattga aaaatggaag
    22861 ttgtcttttt taattctctt taaaagcggg aaacaa
//
LOCUS       DBNENW010000027        22854 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNENW010000027 DBNENW010000000
VERSION     DBNENW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.027"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(94..360)
                     /locus_tag="AC1LW8_000846"
     CDS             complement(94..360)
                     /locus_tag="AC1LW8_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIP4772700.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(360..617)
                     /locus_tag="AC1LW8_000847"
     CDS             complement(360..617)
                     /locus_tag="AC1LW8_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIP4772701.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            976..1431
                     /locus_tag="AC1LW8_000848"
     CDS             976..1431
                     /locus_tag="AC1LW8_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HIP4772702.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1599..3176)
                     /locus_tag="AC1LW8_000849"
     CDS             complement(1599..3176)
                     /locus_tag="AC1LW8_000849"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIP4772703.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGERLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3372..4121
                     /gene="lnsB"
                     /locus_tag="AC1LW8_000850"
     CDS             3372..4121
                     /gene="lnsB"
                     /locus_tag="AC1LW8_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family lipoprotein N-acylation protein
                     LnsB"
                     /protein_id="HIP4772704.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDAFTVFDIYPLIKGYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4483..5676)
                     /gene="cntE"
                     /locus_tag="AC1LW8_000851"
     CDS             complement(4483..5676)
                     /gene="cntE"
                     /locus_tag="AC1LW8_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine family metallophore export MFS
                     transporter CntE"
                     /protein_id="HIP4772705.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PASSL"
     gene            complement(5688..6437)
                     /locus_tag="AC1LW8_000852"
     CDS             complement(5688..6437)
                     /locus_tag="AC1LW8_000852"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772706.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6430..7245)
                     /gene="cntD"
                     /locus_tag="AC1LW8_000853"
     CDS             complement(6430..7245)
                     /gene="cntD"
                     /locus_tag="AC1LW8_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine uptake ABC transporter ATP-binding
                     protein CntD"
                     /protein_id="HIP4772707.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNLERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDSKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVIVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(7242..8111)
                     /gene="cntC"
                     /locus_tag="AC1LW8_000854"
                     /gene_synonym="opp1C"
     CDS             complement(7242..8111)
                     /gene="cntC"
                     /locus_tag="AC1LW8_000854"
                     /gene_synonym="opp1C"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine uptake ABC transporter permease
                     subunit CntC"
                     /protein_id="HIP4772708.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(8108..9043)
                     /gene="opp1B"
                     /locus_tag="AC1LW8_000855"
     CDS             complement(8108..9043)
                     /gene="opp1B"
                     /locus_tag="AC1LW8_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIP4772709.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(9056..10654)
                     /gene="cntA"
                     /locus_tag="AC1LW8_000856"
     CDS             complement(9056..10654)
                     /gene="cntA"
                     /locus_tag="AC1LW8_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding lipoprotein CntA"
                     /protein_id="HIP4772710.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSGAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10794..12098)
                     /gene="cntM"
                     /locus_tag="AC1LW8_000857"
     CDS             complement(10794..12098)
                     /gene="cntM"
                     /locus_tag="AC1LW8_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HIP4772711.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVNGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLSQ"
     gene            complement(12091..12909)
                     /gene="cntL"
                     /locus_tag="AC1LW8_000858"
     CDS             complement(12091..12909)
                     /gene="cntL"
                     /locus_tag="AC1LW8_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HIP4772712.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12920..13741)
                     /gene="cntK"
                     /locus_tag="AC1LW8_000859"
     CDS             complement(12920..13741)
                     /gene="cntK"
                     /locus_tag="AC1LW8_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HIP4772713.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14616..14978)
                     /locus_tag="AC1LW8_000860"
     CDS             complement(14616..14978)
                     /locus_tag="AC1LW8_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772714.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLAILKYLNTVSIENISHSELLNLIENTIPEDRDINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLK"
     gene            complement(15241..16059)
                     /locus_tag="AC1LW8_000861"
     CDS             complement(15241..16059)
                     /locus_tag="AC1LW8_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4772715.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            16294..17832
                     /locus_tag="AC1LW8_000862"
     CDS             16294..17832
                     /locus_tag="AC1LW8_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIP4772716.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WFISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAVVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(17944..18366)
                     /locus_tag="AC1LW8_000863"
     CDS             complement(17944..18366)
                     /locus_tag="AC1LW8_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIP4772717.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(18818..19237)
                     /locus_tag="AC1LW8_000864"
     CDS             complement(18818..19237)
                     /locus_tag="AC1LW8_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HIP4772718.1"
                     /translation="MSKKHGFIIIGVILCICIVASVIYLKMKYDEKEKQKALYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGTMIESERLSELLKPANQLKSPEKIKEELDKKEGH"
     gene            complement(19255..19674)
                     /locus_tag="AC1LW8_000865"
     CDS             complement(19255..19674)
                     /locus_tag="AC1LW8_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HIP4772719.1"
                     /translation="MNKKHVFVIIGVILCICIVVTAIHFKIKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTNFETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAHQLKSPDDIKKELNKKKSH"
     gene            complement(19701..20111)
                     /locus_tag="AC1LW8_000866"
     CDS             complement(19701..20111)
                     /locus_tag="AC1LW8_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HIP4772720.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPANQLKSPDDIKKELNKK"
     gene            complement(20379..20801)
                     /locus_tag="AC1LW8_000867"
     CDS             complement(20379..20801)
                     /locus_tag="AC1LW8_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HIP4772721.1"
                     /translation="MNKKHVFVIIGVILCICIVATVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPAHQTKTPDDIKKELDSKKNDR"
     gene            complement(21052..21747)
                     /locus_tag="AC1LW8_000868"
     CDS             complement(21052..21747)
                     /locus_tag="AC1LW8_000868"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4772722.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTVQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     SHVNVNEITVRPV"
     gene            22036..22317
                     /locus_tag="AC1LW8_000869"
     CDS             22036..22317
                     /locus_tag="AC1LW8_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP4772723.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            22253..22438
                     /locus_tag="AC1LW8_000870"
     CDS             22253..22438
                     /locus_tag="AC1LW8_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772724.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(22577..22768)
                     /locus_tag="AC1LW8_000871"
     CDS             complement(22577..22768)
                     /locus_tag="AC1LW8_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772725.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT         7181 a         4295 c         3344 g         8034 t
ORIGIN      
        1 taacaacgca ttaacgtcgc tattttccct acacttaaat actagtttct ttaattaact
       61 tatcattaaa gttattcaga tattgaatat tcattaatca taatgtgacc atgccgatag
      121 tattaatact tctttatttc gatcatctac ggtatagacg accctgtgct gatggttaat
      181 tcttcttgaa tatcgctcta aatatttagg ctctaatttt tcaaaagatt gtgttatttt
      241 atacggatca ttttttaaag tctcaacaat ttctaaaaat gacttcttta aataagaatg
      301 ttttattttc tttaaatctg atttcgctga atttttaatc ttaaccgtgt aattgctcat
      361 taaagattat cccaatcaat atcatctata tttgttgtac cactattatc tttttctctt
      421 tctcttactt tgtccattgt accagtagat tcaagatata ttgtctcttg tatacttttc
      481 caatcttcta aacctataat cacagcatta ttttcggcat tattgccact aatataaatt
      541 ggttcgtgat tattattaac attttttagt aattgataaa aatcttttct cgcttctgta
      601 gggctagtga taatcatata aaccctcctt tgttttctgt tacgctaatt atagcgtacg
      661 cacttgagta cgtcaatatt accaacgagt tcatcgtatt cttaatacct atatgctatc
      721 gtacacaagc ttttgttctt ttcttccaac ataccgtcta taatgctcaa aaagaaatca
      781 gcagtggaac acactacgtt ccctaccgct ttatcatttt caggcttaaa tatttttaaa
      841 attttgtgag gaatccgctt aaaaaaactc atatcttcaa aaaaagtgac aaaatttaaa
      901 tttttgaata acatcatttt ttagtaaatg ttataatttc tttataatac aaatatcgaa
      961 agaaggcgac tgactttgaa aaaattatgt tcattaattg tagtagcatt agtttgtatc
     1021 attgcattat cagcttgtgg taaagaacaa accaaaacat atgaaggtga tgttagtggt
     1081 aaacatgtac taacttctat cacatataaa gatgataaag ttttaaaaca gtctacaatc
     1141 aacacaatta aatatgacga tctaggtatg gataaagacg aagccaaaaa gttattcgcc
     1201 aaatctgaaa gtattttcaa agaccttaaa ggcgtaaaat acaaagtaga ctataaagat
     1261 aaaaaagcaa ttgaacactt agacatagat tacacagaag ttgacatgaa aaaattaaat
     1321 aaacgtcttg gtgtttcaac taaagaaaat aaagatatta gttttgaaaa acttgaaaag
     1381 caattaaagc acagaggctt aaaagaaaaa gataaaatgg acgacaaata gtttataaca
     1441 taaaaatgcc ctcagataag actaaggtta caaaccttaa ctcatattct gagggcttta
     1501 atatttgaaa ttcttgtgtg accagcatcc actactaata taaaattatt tgcagtaaca
     1561 ctaaaatccg ctgctttcat tttctcgaaa tagataaatc aactaatcaa ttttaattta
     1621 taatcatgta tcgtttgtaa ctcaccatcg acttttcgat atacaatatg atcagcagta
     1681 atttctgtag gactggatac gccaacagct gctgcaatat tgaataagcc ttcatgcaaa
     1741 cttgttacat agtttgtgac acgatattgc ttttctccaa caatcaatgc tttttctttt
     1801 ttagcatctg ttgttgcaac accaactgga cacgtattca tgtgacattg ttgactcatt
     1861 atacaaccga cactaatcat catcccacgt gcgatattta caaaatctgc acctaaacct
     1921 agtgcaatcg caattttatc tggtgtcact aacttaccag atgccgccaa tttcacttta
     1981 tctcgaatac catatttttc taacatgcca gacacaatag gtagagctgt aaatagcggt
     2041 aagccaacac catcttgtaa ttcttggaat gttgcaccag taccaccttc accaccatca
     2101 atcgtaataa agcttggata cttatctagt tccaccatcg tacgtacaag tgtttcaatt
     2161 tctgaaactt tgcttactac aattttgaat cctactggtt tttgacctaa ttgctgcaac
     2221 tgatcgacga aacgaatcaa atcttcagca ttatgaataa attcgtaacg gttaggtgaa
     2281 ttgattgttt tataaggttc aacatttcgg attttagcaa tttcttcgtt taccttttca
     2341 gcttccatat gaccaccacg agtcttagca ccttgtgcca acttcagctc aaatgcgcgt
     2401 acgttagata actgtgcaac ctctttaaat aaaccttcac taaaattacc ttctttatca
     2461 cgaacaccaa ataaaccggg accaatttgg aaaatgatat ccccattacc ttttaaatga
     2521 tattctgata agccaccttc acctgtattc atccaagtgc ccgctttagc tagaccttta
     2581 gataaagctg taatagcatt ttttcctaaa gcgccataac tcataccaga ttgtcctacg
     2641 atacgtttta aaataaatgg atgttttaaa cgttcaccta attttattgc atggtcatca
     2701 cttaagtaat acggatcaat ctttgtcggt acacgatatt cttcacgact aaataaacgc
     2761 tcattcgcga ttttataaat gaatgttgat aacaatgttg tattatctac tgaaatctca
     2821 ttacgttgca tcggaaacat tgtgttctgt atgtaaaagc cgtcttgata atctttagta
     2881 gtaccgaagc tggtcatacg agagttatat tttccagcca aaacgatatt tttatagtca
     2941 ttacgtgaaa aaggtttccc ttcattatcc ccagaaaata aatactgacg taattccggt
     3001 cccatttttt ctgaaatata tctaatacgt gctagtaaag gataattcct taatacacta
     3061 tgttgtgatt gtcttttatc tttaattaac caaataagcc cgataacaat aaccgtaagc
     3121 aggaagccta caacgataat gttaactata aattgcatga ctgtaagaaa cgtcattaca
     3181 atccctcccc caaaaaattt caattcaata tttatgatac accttacaaa ataaaacaca
     3241 atggaagcgt ttcattttat aaaacaattt tatgatatgt ttttcatttt aaattttaat
     3301 gtataaaaca tacaatacaa agtaatatgt gctaaagtat ctatataata caactattta
     3361 agaggtatac tatgtcaaat acaaataaac attacataga agaagaatac gctactgaac
     3421 aatctcgttt tttcaaacgt gatattggat ttattttcct ttatatattt ttggttaaca
     3481 tcttgccaat cattatcgtc tttttaactt ttgggttagt agctgtattc actcaacaag
     3541 ccccatgggg tttctctttt cctatttcaa tgaacattag ctatttactc gcagttatta
     3601 tttcactagc tatttttcgt cggatgcatg aagatgcgtt tattccaatg atacgttcgc
     3661 aaatcgttgc tagtgctaaa tataaatggc atattcttat atcatttaca ctaagtgtcg
     3721 tactcttttt acttttaaat catttcaata ttttcgcact aaatgtagat gcctttactg
     3781 tctttgacat ttacccgctc attaaagggt atggttccat cagtttaata gctatatttt
     3841 cgttaaccat tatattaaat actttgattt gtcaacttat tttccgtcat attttaattc
     3901 aagaattaag cagattaatc cctttaaaga ttacaatagt attatccgtt attcttgaaa
     3961 cactgtacta ttatccatac attaatacat ggtgggaatt cattccagca ttgatactag
     4021 caagtagtgc gacttactta tatttaaaat cataccgtaa ttttatggtg agctattttt
     4081 atcaattagc agttatactc gttttgcata ttttcatgta aaatatttca gagtattatc
     4141 cattatccat acacatcaaa aagcacactt aaaagtcagt atcgcaactc acttctaagc
     4201 gtgctttatt tagatatact gtcgttctaa aaacgagcca tatagagagc ctgagacata
     4261 aatcaatgtt caatgctcta caaagttata ttggcagtag ttgactgaac gaaaatgcgc
     4321 ttgtaacaag cttttttcaa ttctagtcaa ccttgccggg gtgggacgac gaaataaatt
     4381 ttgcgaaaat atcatttctg tcccactccc atgaatcgcc atgctcaata taccttttaa
     4441 aatttaaata ttcattaact taatatgctt taaacttttt cattaaagac tactcgctgg
     4501 gcgtggtgtt ggttttttca ataaactcat aatcattatt acaatgacta acataccaca
     4561 aattagatac atccatttaa atcctactaa atcggaaata ggtcccatca atgcgccacc
     4621 taacgatata cctaagtctg cacaggcaat aaataaacct aacaacatat tacgacctac
     4681 ttttggtaag acgaagctta agtatgatgt taatgttggg tacaccattg cttgtgtcat
     4741 tcctattaat attgcactac catagaaaat aattgcacct acttgcggac caaatgccac
     4801 tacaaatgac gcgatcacta ataatgatag tacagatacc atatatttag gatgccacat
     4861 accatctgac ggaatgtatt tccttaagta aaatcttgcc acaacaactg cgatggcctg
     4921 tattgtcaaa aagattcccg cattcgcgaa tcctaaactc actgtgtata acggtacaaa
     4981 tgtactaact gcaccaaata caatcgatgc aacaatcatg ataataccac tgttcaacaa
     5041 ctctttattt ttgaaaaatt gcgcaaaaac agttacagcg ttaaacggca ttttttcaat
     5101 cttatctgac gtatcgggtt cctgttcagc aaaggtcacg cgatagccaa agaatgttgt
     5161 tgttaatgcg ataaagataa tgacaattgc aaatagtgaa atattatttg cattccaaat
     5221 acctacggca actaatggtc caattaagtt tggaatcgtt gaaaatagcg agtacaatga
     5281 tacaccttca gaacgatgtt cctctggtaa tgcatcaata atacctaact gtaaagacat
     5341 agaaaagaat gccgtacaca caccttgcat aacacgtgct acgaaataac cttctaatcc
     5401 agtaaaacca taaataatta aagctatggc attgatgatc aaaataattc ttaatacttt
     5461 aatgggaccg acacgagcaa taatttgtcc tgcccatggt cgaaacacca ttgctgttaa
     5521 catgtatgcc cccataacga taccgataac cgtattcgtt gcgcctaaat catgccctcg
     5581 taacggtata aatacgttta agattgcatt ggcactaaag aacatcaatg ttaatatata
     5641 taatctcaaa aagggccaag ccattgcacc tttcattatc tcactcctta gaatgatagt
     5701 tgtttttcta ttaattctct tgtataagca ttgtcacttt tatgcaatgc gcttgtcgga
     5761 atttgttctt ctatttttcc gtttttaaaa ataattaatt gatcacataa atacgtggca
     5821 gcttgaatat catgtgtgat aaaaatataa ctcaactgac gcgtttcacg taaatgaatc
     5881 aataaatcta atatttgtgt ttgaattgac atgtcgagtg aactaatggc ttcatcaaac
     5941 aaaatatatt tagggttaat acatattgca cgcgcaatcg caacacgttg cgcctctcca
     6001 cctgataaca tattaggata tttatccatg tatgccttag atagaccgac ttcttccaac
     6061 aatgtaattg cttgaacttc cataacatct ttaggttgtc catcacattg acacatcact
     6121 tcaaataaga tttctctaac agtctgaaat ggatgtaatg atgacgtata atcttgaaat
     6181 acagcaccaa tttgatgacg tctcactttc ttcttatgca tcggttgatc atttaaggtt
     6241 acacaacctt tatccggttt ctcaatacct aatatcatac gactcaatgt cgatttaccg
     6301 ctaccacttt ctccgataat cgcaatcgtc gcaccgattg gacactcaaa tgacacacct
     6361 ttcacgatag gtgttcgacg acgcttaaaa acatgtgcgc tttgatatga cttttcaaca
     6421 tctttaattt taatcatgta catcacccct catcacatgt ttaaaatgat cattaatctt
     6481 cttcttcgtt gataataaat acttcgtata aacatgttct ggatgatgca agactgattc
     6541 acgtgtccct tgttcaataa gctgaccatt tttcatcaca ataacacggt ctgcaatctt
     6601 gttaataacc gttaaatcat gtgaaatgaa aatcatcgca cagtcaaagt gttttttaat
     6661 atctataaat gcttccagta catcatattg tgtaattgta tctaaagccg ttgtcggctc
     6721 atcagcaatg attaactttg gtttcaaagc taacgctaaa gcaatcatca atcgctgtaa
     6781 cattcctcct gataacatgt aagggtatga ttttaatata cgtttagaat ctttcaaact
     6841 taaataatcc atatattcaa tcaatgtctt ttcaatttct tgtgtagaca ttgacgtatg
     6901 tactttcata gtctcaaaca tttgtttacc gacagtagtt gatgggtcaa aggcacgact
     6961 accttgttgc atgaccatcg caatgtcttt accacggtac tttttcaatt gcgattcaga
     7021 taatgacaac attgatgtac catcaaagat aatttcacct gtcaccccga gtcgttcgag
     7081 attcaaacca ataatcgatt tacaagtgat tgatttaccg ctaccacttt ctccaataac
     7141 gcctaaagtt tcacccttag ttaatgtaaa attcacatca ctcacgagtg gttgatctgt
     7201 ccaggtatct gtaatcgtca aatgtttaac tgttaacaat gtcatgattg caccactcct
     7261 tttttcacag aacgaagttt atctttagaa gagatgcggg gatcaatagc aatttgtaaa
     7321 gcatcggata agaagttaaa tgccatcact ataatcacta tggcaatacc tggcgcaaac
     7381 atcatttcag gatgtgtaaa catcactttt ctagcttcgt taagcatcat gccccactct
     7441 gcagtaggcg ctttgacacc taatcctaaa aatgaaaagc cagatatttg caagatcatt
     7501 gaacacatcg aactactaga gatgatagca atatctgcta atgttaacgg cataatatgt
     7561 ttgtgaataa ttttcatatc attcataccg attgttttag caaatcttac atggtcagaa
     7621 gcagtgtact gcataacact tgtacgtata acacgacaga accatgccca acgcgtcaaa
     7681 ataaatgcca tgataatatt ttcggcaccc ataccaaaca atgcaattaa tgctaacgtt
     7741 acaacataac ttgggaatgc caacataaca tcacacgcac gcatgattaa ggcgtcaaca
     7801 aacccttgga aatatcctga taagaatcct aaaatagatc caataagtac agaaacaaat
     7861 agcgcaacaa agacatataa caaacttggt ctaatcgcat aaattaacct agttaaaata
     7921 tctctaccta aatggtcagt acctagtaga tgttgaaaac tcatgccagc aaatttgttt
     7981 gctgtatcga tatggttagg atcataaaat gtcacaagtg gtgctgctaa tcctaaaaag
     8041 acatataata caataatgcc taaagcaatt actgcacctt tatcttgtaa taatcgtttt
     8101 aaaattatca tcgtgcgccc tcccttaatc ttggatttaa tagcgcatta atgatatctg
     8161 ctaatgtatt aaatacaata aataataccg ctacaattaa tacatatgct tgaataactg
     8221 gaaaatcgtg ttcaagtatt gcttttaaac ttaattgacc tagtccaggc catgcaaaga
     8281 tatactcgat aacaactagt ccacccatta tcattggtat agacatacaa aagattgata
     8341 ccgcaacttg taaagcatta cgcaacacat gcaacattaa tgtgatagat ttcacaccgc
     8401 ttgctcttaa ataaagtaca taatcttcat ttaattgttc caccatcgag cgtctaacat
     8461 ttctaaagta aataccagca taggcaatcg taataacgat cactggcaat atgtaacttt
     8521 ctggacctgt taatccagaa gtcggcaata tgtttaactt cactgaaacg taaataataa
     8581 gtattgaagc tatccaatat gatggtaatg cagttagaaa gaaagccact gaacgtatcg
     8641 cacgatcagt gaactttcct ctttttaatg cactaactac acctaaaata attgatgtaa
     8701 tcatcaccat aacacttgaa attattgtta atttcaatgt attcataaat gctggaccaa
     8761 tgcgttcagc aacaggatca cctgtaatgt agcttgtacc aaaattaaat tgcatcgctt
     8821 caagtaacca atttttatat tgaattaata atggatcatt gaaaccgtac ttctcattcg
     8881 tttctgcaat caactctggt gttacatttg gcgtcccttg tgcatgtaaa attgtcacag
     8941 ctggattttc atttgtaata tacgtcaata gaaatgtcat aaaacttact acaatcacta
     9001 atggaaacat gagcgcaata cgttttaaga taaatttgaa catctaattg ctcctttatt
     9061 tatactgcat ttcattgaat ggtaattcat actgtgattg tgtgaatgat actttttcta
     9121 aatctttcgg cgcaacaact gtcatactac cgtgtgaaat agggataaag ataccttcat
     9181 catcaatttg tttcaaaatg tttttataag cgcctgaacg ctctttaccg ttttggattt
     9241 taaatgcgtc atcaatgctg ttgtatattt tatctttgtt ctcaatgcct gatgttgcac
     9301 tttcataacc atttttcgct ttaaatgctg caatagtact ttgtggatcg tacaataatc
     9361 cccaagtttg gttgaacatt aagtcataat caccagaagt acgacgttca gcaattttat
     9421 ctgatgtttc gccattgatg tttaacttaa tacccatttt cttaaattct gcttgtaagt
     9481 attctgcttg ttctttttga cttgaagaac ctttgtcata gtacattgcc atttcaaggt
     9541 ttttaccatc tttttgacga acatcgctgt ctttaccttt cttccaacca gcttcatcta
     9601 ataatgattc tgctttttta aggtcatact tacgtgttgg catatcgaaa ttaatatctg
     9661 ttacattttt cgcaaataat tgagttgctg gtttttcttg accatctaaa atttctttgg
     9721 caattttatc tctgtttacc atatgaccaa tcgcttgtct gactgttttg tcactcacag
     9781 cgttatcttt tttaccagaa ttgacaacta acattttcgt attcataggt tgactacgct
     9841 taacttgata gtcacctgta tctttcaatt gttttaaaga gtctttgtct aagctatccg
     9901 tacctctatc atctgtgaag gcaaagttcg tttcaccttt tttcattgat aggaatgctg
     9961 tttcaccagc aggcattact tttgcttgta ctttgttaag tttagacttt tcgccccagt
    10021 attgatcatt tttgttaaag tctgcagact catctttttt atgttcacct aatttaaatg
    10081 gaccagtacc atcgaacttt ttaacgccat cttttgttgt accgttttta aagtcttttg
    10141 gagacacaaa tacatatgga cgaggcatcg ctaattcagc caatgcaggt tgatatgctt
    10201 ctttcaaatt caattcaacc gtgtacttat ctttaacttt aacattgtca attaatgttg
    10261 aaatctttaa ccaagaatgc aattttttat tttgttgaac cgcatcaata tttttcttaa
    10321 ctgcgtcagc atcaaatggt gtaccatcat ggaatttaac gtcatctctc aaatggaatg
    10381 tatatgtctt cccatcttca gacacatccc attttttagc tagtaaaggc ttaattccat
    10441 ctttcgtgtt acgtacaagc ggctcgtata tcatactttc agcagacatc gatccaccgt
    10501 aaacatgcgg attcatatca ccgatatctt taaccgtcgt atacgttaat tgcttgtttt
    10561 cttttttctc ctctaaacct ttattaccgc cacaaccagt taaaattagc cctgatgcaa
    10621 gtaacattgc actcatttta gttagttttc tcatttgctt ttcctctttc taaattgata
    10681 agttgcatta cttaatcaaa tcgtaatgat tatttttctc aattatttgc ttatttttat
    10741 gtaaatgtca aacataattc aataattttt ttctattata aaacttcaaa cccttattgt
    10801 gaaagcgttc tattgatttc caaaaatttt gtgacgagtg ctttatctcc ttcaaataga
    10861 tttgtattaa attgagaaga taagtgttga tcttgatgca tatccttgta cgcctggcaa
    10921 cttgcttcat agcgagttag gaactgatca atcattggcg ttttgatacc tagcattttc
    10981 ccaatatgct gaatcatcgc cgttctgtaa taatcttcac ttggcattct tggaatttga
    11041 acaacatcct gttcattttt atagacttgc ttaaatggta cagctgaaaa atcaaagtaa
    11101 tgtccgtttt cgtctggctg tgaaaatgga tcaatgagga ttgcggtata tcttacataa
    11161 agcagatatt cttgtaagat atctggcaag atttcgaaat gctcaatatc accttcatcc
    11221 aaagtttcag gacgtactgg ataattttcc ttcaccataa attgaagcag gttgactgac
    11281 ggcactctaa atgcttgtaa aatagccatc atttccttcc acattaaacg catttcacgg
    11341 attagtgtca tcgttatcgg tccttcagga aataacttat acacataaac cggtacatcc
    11401 gtcccttcga aaatggcttt caatgaaaag tcattcataa atagtggtgg gtgcacataa
    11461 agcgaactgt ttcgcgtttc agcgtgcagt ggcgattcaa cgacttctaa ttgaattttc
    11521 aattgctcag ctaaagcaga gattcgttga cacattgttg agtttgaatg tgtcgatccc
    11581 atgtacaatt tcttttttac acctgttgtc aacacatgat taggcgcttc tttatcaaca
    11641 atacgtgtat cgccaagata agttgagaat gaaatcactt cgatatcttt attaaattta
    11701 gacataaatt gttcgacaat catttgcgaa ccaaatgtcg gtgatattaa aatgacatgt
    11761 ttgacacttt gcaaagtttc taacgacaat tgctgtagtg tgtcataata agcatctgct
    11821 gtgcatgcca tgacaaccgt ttcgtattca ccattaacgt ttttaacgtc tttatacaaa
    11881 cgattaattt caaacttacc ttccagatgt tgatgcgcct cgttttgtat tttgacttca
    11941 aattgtttct cttttttata cgcttgatat aagcgttttg atttttctga tgttgaggca
    12001 cgtccaacca tatcaatctc ataatctgat tttaaatagc aaatattcgc taattggatt
    12061 gcgaccggac cagtgcctat cattaataat ttagacatcc gtaataccta cctttatagc
    12121 tgctttttta taaagtgcta tatcaaaaat ttgctgtggt ctcatatgtt tattcacaca
    12181 ttgccactta tcttccgctg tttcttgtga cggataatta aatattgctt tgatgccatc
    12241 accaaagcgc attgcaacta cgacattttc atttgttaaa tcatataatt cttctaaaat
    12301 gctgtacttt aaaggaattg tcgagctgaa tatgatatgc gtcacatctt tgatatcttt
    12361 aagttcagat accttttgat ccgtaattgt tatatcttca tttggtgcta agacgttaac
    12421 gattctgcgc cctaggtcaa cggcttgtgg atcaatatca ataccgataa ctgaagcacc
    12481 tgtttctttt gctacttgaa ttaacgtcat tggatatgca cctgaaccaa ctaacaataa
    12541 tttgtcattg ctagctatac tacattgtcc aaactcttca tcaatacaat gttctatatt
    12601 gtcaaagtaa cctgtggtag acgcctgtcc gtctaataga cgtctcgctc taatgacttc
    12661 cacttgttta acacactgcg ctgtaatata ttgcagtgat tttattaatt gtgctttttc
    12721 ttcaacgtca cgccaagcat taaattgttg ttcataaata ggatttaaaa taaattcact
    12781 atagtcatcc atcaatgttt ctaatgcttc gatatattga tcgtcttgta atacacgctc
    12841 gtaatgcgct tcaaactttt ctaaatattg ttgtaatatc aatttgattt cattattaaa
    12901 gttattcatt gtaggctcct tattctatat atgcttttcc tgtagctaca gttgtaacct
    12961 gtcctttaat tgaagtttga tatcccaatt gatgacatcg ctttgatgtc actaaaatac
    13021 tgccccctgg ctgatgtact gtaaaatctt tgcatgcgtc attacgttga taattattaa
    13081 aaaccccaat tgatgctgta ccagaaccac agctattttc ccaaattaaa ctttgaattt
    13141 ctggtatata gattaatggc tgtaaaaatt gacgttgttc atcaaaaagc atcataccta
    13201 ctgttttata tttgtgactc cattgttgct cacgcacaaa cgcttcaacc aaatgttgaa
    13261 tttctgttgt tacttgttta actggaatca cataatgtac atatgtttca taaataattt
    13321 ctattgcttt ccatgaatga ttacccatat taattgttgt tggcacaaca cgatgggctt
    13381 gtggcatttg aacttcatag tattggcaat catgaattgc gcattgcact aaatccgaac
    13441 agccagacac cttcacctta aactgttggt ctttaagcaa atgactttct tgcaaatgat
    13501 gtatatatga catcgtcgca ttaccgcaaa attcattacc gctcataact aagtgaaaat
    13561 cattgccatc atcattttgt gttgattcta taaagcctac ctgttcacaa catacatgtg
    13621 ttgcggccat caactgattg gcgatagatg catattcact agcatcatgt tttgaatgaa
    13681 caagtatcgt catattcccc gaaggattat acttagaaaa ttctataacc tgccgattca
    13741 tacgacactc ctttagatgt atttttagag cctctgtaat tttaattact agaggctctt
    13801 atgcagttgt tgcgaagcaa acaactgtat tcctctaaac ctgtaatttt ttagaagatg
    13861 aacctaatag ctctatctag taggtaagtc atgctaattc gcaaaagcga aaaccatggc
    13921 tcttatgcag ttgttgcgaa gcaagcaact gtattcctct aaacctgtaa tttttaatag
    13981 aagatgaacc taatagtttc ccatctatta ggtaagtcat gctaacccgc aaagtggaaa
    14041 gcatgactta gtaatcggtt gttgtatcaa catgcaaccg taaaccttat ttaaagttgc
    14101 ttcttgtggc tctacaagcg aatgcagttg tccgttaact acattcactt agatgctttt
    14161 actgttttca cctcaactaa aacttcaaaa aagttgcaga aaacaaatcg taactattac
    14221 gatttgatta tatactatat tagttgagaa tcattgtcaa taaaggttgt aaatgaatgc
    14281 acatttatat tttgataatc cttccataaa catcgtattc tcagcacatt caagtctttt
    14341 tgaaaatttt tatcaataat tgaatagaag ttccgttctc gtcagttcaa aattttacgt
    14401 aagttgtctc tgcgtattgc tttagtgaga ataacccaca cttgtggaaa cgtccttttg
    14461 ttaatctaca atggatcccc ttgtctttcg ctaacatttc ctatcaccaa tcctgtaacg
    14521 aattttcatc gtcaagttgt tgcccatgca atgcgcacca aaaaaagctt tgtacaattt
    14581 cgtacaaagc tttaaaaatt caaataaaaa tatcattatt ttaatgaact agccatatca
    14641 gattttattt cgtttgctaa tacttgtaat tcagggaaat aattattagc aaagccaatt
    14701 attattttat tttttacaaa cgtgttaata tctcgatctt ctggaatcgt attttctatt
    14761 aaattcaaaa gctcactatg acttatgttc tctatactta ctgtatttaa atattttaaa
    14821 atcgcaagat acaattcatc aaatacttca tgttcaaaat caaagtccaa taataataaa
    14881 ctaacggaat tagtttctaa ctgattatct ccaattttat ctgcaatctc tcttatagct
    14941 tgatatagca atctaaaact ttctttttct tttaacataa aattctcctt aatttattag
    15001 aatagacaaa atacagctta ttttaaagat actccaaaag tttgaactcg agattctgat
    15061 aaattagatg cacgacctac aatacgttaa aacaagcttt aagcaataca cttcaaatga
    15121 acaatatgtg attttcaaag attaattaat ctcggcttta aatgtctttc attgcgacaa
    15181 tatgcataca ttgcctactt catgccaaat tcttttccac tggatatcga ttttaaataa
    15241 ttattccaat gtccgcttcc acgaatcatc actcaacatc tcgccaggcc atgtgtaagc
    15301 cataacacca ccgtcaatac gaattgtttc acctgtaata aatgaactct catcagatgc
    15361 taaaaagaca acgagcttgc ctacttcttc tggtttacct aaacgtccca gcggtgtcat
    15421 ccatttctga ttttctctga atgctttgcc ttccttatct tcactcgtac ctgtcagttt
    15481 atctactaac ggggtttcaa tcgtacctgg tgcaatcgca ttagctcgaa tgccatctct
    15541 accatattcg attgcaatgg atttcgtaaa attaatcact gcccctttcg cagcattata
    15601 tcctgagcga tacaaatctg ctgcctgtcc tgaaaatgaa gatgtgttaa caatagagcc
    15661 acctttagtc atcattaaag gtaacatcat tttcgtcatc aaaaatgttc cacgcatatc
    15721 tacattcata atcttgtcat atacatctgt tggatactca tgaattctac cagccgcatt
    15781 atccacaccg gcattattaa ataacacatc aacatgccca atctgttctc taatttctga
    15841 tgcaaaattg tcaatttgtt gttcgcttgc tatatccaca atataagcct ttgctttgtc
    15901 accattactt ttaattttat cgacagtctc cgataccgct tcagctatgt ctaccgccaa
    15961 tacatacgca ccttcttgag ctaaagcgat tgcagaagct tgtccgatac ctgtacttgc
    16021 tcctgttacg actgcaactt tattttccaa acgtttcatt cagtcactcc tatacaacat
    16081 atcttaaact tacagtcctt attttccact gttcaatgct tcataaacct aatagataag
    16141 tctccaggca gtcttatcta ctctctgcta tacaatttct attgaataac tcaaaatatt
    16201 taacaatatt agatttttca ttttctgaaa attgttactt ttgagagtat tttttgttac
    16261 actttaaata tttaacaaag gggggaatca ttgatgacat caaaacatca acaaaagggg
    16321 tctatcgtca atcgtttctt gaatagtgtt gaaaaaattg gaaataaatt gccagatcct
    16381 agcgttttat tctttttaat gtgtgtgggc ttagccatta tgacgtggtt tatctcatta
    16441 tttaatgtat ctgttaagca tccaggtacg catcaaacca tttatattaa aaatataatt
    16501 agccatgatg gatttacgat gataatgaat gatacgatta aaaatttctc agagttccca
    16561 gcattaggct tagtactagc agtgatgatt ggtattggtg ttgcagagaa aacaggatac
    16621 ttcgacaagt taatgatttc tgttgtgaat cgcgcaccac gtttcttaat tttaccgact
    16681 attatattaa ttggtatttt aggtagtaca gccggcgatg ctgcgacaat tatcttgccg
    16741 ccgctcgcag cgatgctttt tattaaaatt ggctatcatc ctatcgctgg actaacgatg
    16801 gcatatgctt ccgctgtagg tggctttgcc gcaaatatag ttgttggtat gcaagatgct
    16861 ttggtctatt catttacaga accagcaaca cgtatcgttt cagattctat taaaacaaac
    16921 gttgctatga actggtactt tatcgcagcg agtgtcgttg tattacttcc tacgatattg
    16981 ctagttacaa ctaaactgat tattcctagg ttaggtcaat atgatgatcg tttaatgcat
    17041 gatgatcatg aagaaacgtc ttcacatatt actgacaaag aagctcatgc tttaaaatgg
    17101 gcaaatatca gtttcatagt cacaattatt ttattaatta ttacagccat tcctgaacat
    17161 agctttttaa gaaatgctaa aacaggcagc ttactagacg atgcgccatt aattaacggt
    17221 gtcggattaa ttattcttgt tgtattttta gtacctggat tagtttatgg gattttaagc
    17281 aaagaaatta aaaatacaaa agacttaggt aaaatgttcg gcgatgctgt aggatcaatg
    17341 ggaacattta tcgttattgt gttttttgca gcacagctac ttgcgtattt aaagtggagt
    17401 aatttaggaa ttatcgctgt tgttaaaggt gccaaattat tagaacatca aaacggcatt
    17461 gtattgatac taggtattat tgtactgagt gcaatggtca acatgttaat cggaagcgca
    17521 tcggctaaat ggggtatttt aggaccgata ttcgtaccaa tgttaatcct tatcggcttt
    17581 catcccgcat tcacacaagt catttaccgt gtaggagatt caattaccaa tccaattaca
    17641 ccgatgatgc cgtacttacc tttattatta acttatgcac aaaaatacga taaacgcatg
    17701 aaactcggag ccttactttc tagtttaatg ccgtattcaa tcgcgctaag tattgtgtgg
    17761 acagtatttg tcattatttg gttcctatta ggtatccctg ttggaccagg tggaccgata
    17821 ttcgttaaat aacagtattc aaagggataa attaaaatgc cactatacaa gatttgacct
    17881 gtatagtggc atgatttgtt atgatatagc gttcgagatg aaactccgta tttatatgaa
    17941 gaattatagt ttatcactaa taatatttag cctgttccaa ccgttaattt ggtttacaac
    18001 aaagacaaga tcaataattt gttcttcatc ataaaatgat tttagccggt caataatttc
    18061 aaacttctta aagtctttga ttgaatttaa catttctgca aattcaagcg tcactttttc
    18121 ttgatcagta aataaatcta aatgtctaaa actcaagact tcatcaattt tcatttgtgt
    18181 tacacccaat tcctttaatt cttttttatg gatatcaaca caataatgac aaccgttaat
    18241 ttttgaaatt aataacttaa tcaatgcact aagttcatca ttaacgggtg attgatgaat
    18301 aaacttttca ccttggctca ttacagacat taactctcca ttttgtttct tgtaattata
    18361 cggcatttgc aatctctcct ttacattgtt taataccaat ccagtgatac tacacttcga
    18421 acaatattgg atagaatttg aaattctgta taacatcttg tcaatctatg ttttagttaa
    18481 aacatttagc gcttatattt aaaaaatata taaaatatga aaacagattg aaagtatatt
    18541 ataccttgct ctaccctttt ttataaaaaa tcacacacat ttgagaaaat ttgataatgt
    18601 caataaacca cgtcatcgac attgttattc gatatatatt gttcttataa tggccaaact
    18661 attattccat ttaaattaac gtggttcgtt tgtgttaatg tactgtacaa aactggggaa
    18721 gtattggtga tgatgaagtg attgatatta attgcgaaac accttcgata gcaatgacat
    18781 agattattgg aagattaaat caaaaataaa gaagacccta gtggccttct tttttgtcta
    18841 attcttcttt aattttttca ggagatttta attgatttgc tggttttagt aactcactta
    18901 atctttcact ttctatcata gtaccaccaa actgataatt atgttctgga gacccatatg
    18961 caacaaaatc agcttttttg ttttcattga tgtaaccttc aattacggca tcgcccatag
    19021 gtcctctttt taaacttgtg aaatgcacag atttaatcgt gtttggttct ttggtattat
    19081 gcttgagata gagtgtaata cgctcctgtt gttctttgta gtaaagtgct ttttgttttt
    19141 ctttttcgtc atatttcatt tttaaataaa tgacggatgc aactatacat atacataaaa
    19201 tgacaccaat aattataaaa ccatgttttt tggacattaa agcaactcct aactttagtg
    19261 actcttcttt ttatttagtt ctttttttat atcatctggt gattttaatt gatgcgctgg
    19321 ttttagtaac tcacttaatc tttcactttc tatcatagca ccaccaaact gataattatg
    19381 ttcaggtgta gcataagcag taaaattatc ttttttgttt tcattgatgt agccttcaat
    19441 cacagcactt cccataggac ttgtttcaaa gttagtgaaa tgtacagttt tgattgtgtt
    19501 tggctccttg gtattatgct tgagataaag tgtaatgcgc tcttgttgtt ctttatagta
    19561 tattgctttt tgtttttctt tttcatcata tttaatttta aaatgtatgg ccgtaacaac
    19621 tatacatata cataaaatga caccaataat tacaaaaaca tgttttttgt tcattaaagt
    19681 aactcctaac tttagtgact ctacttttta tttagttctt tttttatatc atctggtgat
    19741 tttaattgat ttgctggttt tagtaactca cttaatcttt cacttttaat taaatcacct
    19801 acaaattgaa agttattttc aggtgatgca taggcaacaa aatcatcttt tttgttttca
    19861 tttatgtaac cttcaatcac agcacttccc ataggacttg tttccaattt tgtgaaatgc
    19921 acagatttaa tcgtgttcgg ttctttagtg ttatgcttga gataaagtgt aatgcgcgct
    19981 tgttgttctt tataataaat tgctttttgt ttttcttttt cgtcatattt cattttaaaa
    20041 tagatacttg caacactaat taatatgatt aacataatac tagttaatat taaaattttc
    20101 tttttcgaca tctatcacca gctcctaaaa tgtggtggga ttttatacag tttacatatt
    20161 tatgatacta caaaacacac tcttacaaag aaaatctata attttaatct aggtcaagga
    20221 aaagcagcca aaagtgttta tataagatca acgaaatatt gcgggtataa agtggttaac
    20281 agataaatac agtggaatgt tgtactaaaa attcaagttt ttattttttt gtgtaagtgt
    20341 gattagcttg aaaacaaaat taaaacgacc gcatagtttt agcggtcgtt ctttttagaa
    20401 tctagttctt tttttatatc atcaggcgtt ttggtttgat gtgctggttt taataacgtg
    20461 cttaaatttt tacttttgat taaacttcca ccaaattgat aattatgttc tggagaccca
    20521 tatgcaacaa aatcagcttt tttgttttca ttgatgtaac cttcaattac ggcatcgccc
    20581 ataggtcctc tttttaaact tgtgaaatgc acagatttaa tcgtgtttgg ttctttagta
    20641 ttatgcttga gataaagtgt aatacgctcc tgttgttctt tatagtaaat tgctttttgt
    20701 ttttcttttt cgtcatattt cacttttaaa taaatgactg tagcaactat acatatacat
    20761 aatatgacac caataattac aaaaacatgt tttttgttca ttaaagtaac tcctaaaatg
    20821 tggtggaaaa tgaaaaattt tttgtaatct ataattatga acattacaga ttataaacca
    20881 taacactaac atcgccccat tcattaactc gattgttaaa atcaccagtt agtgaaaatt
    20941 gtgcaaggaa taattgccta tcatgctttt atatcattat tgccttacta atattaacaa
    21001 aaccgatagc tattacatta aatgtctatc tcccacacct caaacacatt tttatacagg
    21061 acgcactgta atttcattca cgttaacgtg actcggttgt gttaatgcat acaataccgc
    21121 ttctgcaata tcttgtggat caagcttttt acgatcactt ggattatatg cggcagttat
    21181 tgctgtatct accattcctg gtgaaatgct tgttacttta acacctgtct ttgccaactc
    21241 tttttctaat ccttgagtaa tagtgtgaac tgctgctttc gtcgcactat aaatcgtact
    21301 acttttcgtt acttcaaagc cagaaataga tgcaatgtta ataagatggc cacttgattg
    21361 ttctaacata gttggtaatg cagcctgtac cgtatataaa gtgcctttga tattcacatc
    21421 aatcatacta tcccactcat ctacttgata atcagtaatc ttagacgaca acatttgccc
    21481 cgcactattg ataacaatat ccaaaccacc gaatgtttgt tgtgcaattt ttatcaattc
    21541 atcgacttct tctttattcg ttacatctgt tggcactacc ttcacactat cttgtgacaa
    21601 ttgattcgct acgttttgta atttttcttt atttctacct gctaagacaa ctttcgcccc
    21661 ttcttcatgt agtaatgttg caattgcttc tccaatacca ctacctgcac ctgtaactac
    21721 tgctatttta tctgttaata ctgtcataat gatcgactcc tttgattctt tttatttttt
    21781 cagggtaaat cataaataca tattactttt aaaaagcgta tcacaattca tataacggtc
    21841 ataataactc gcttcatttt cgtgaataaa gtatattcca agccattcga aatatacaat
    21901 taatcgttgc ttatattttt tatttttaaa aatgttgaaa aatcgtcact tctttattgt
    21961 aaaaacatta tattagtact aaagttaata ctgtgattta tcattcgatt gaatgattag
    22021 agggaggaat aaaacatgac atatcatgag cgtgttttag cattaagagc tgaaagtaaa
    22081 agaaccgcat ttgattttcg attcgaagat ttatttagca aagaagaatg gctaagtatg
    22141 tcgcttgcag aaagacaaaa agctgaaaaa gcatttcgac acgaagttaa aaatatggac
    22201 gatgtaagaa tgcccttctc aagtgtccat gacgcccaag taaaattata taatgttgta
    22261 tattcttata acggcattaa acgtaatttt aaacaagttg aaaatgaagg attctaatat
    22321 catttcgttt atatatagca gacatgatag aattttatat gtaaatcttg taggtaatcg
    22381 ttttaaaaat aatataagca tgactaaagc cacatccaat ataggacgtg gcttttagta
    22441 tcgttccaga tttgtgggga gttagggttt acttaattaa aggggtctgc cctcaattgc
    22501 tcaccaacga gggcaagtta catcaatcac acttcaattg ccgcagacta ggggtagtaa
    22561 tcattggcaa taagaactag ttagtgacta acgatttacg ttccatagca aagtgataca
    22621 aacgctcagc atcaatgtaa ggcatagaat caatagttag gtaaccatct ttgatgtcac
    22681 caattaatcc ttgatttaca ccagtgttca cgtaaatatc atagctactt acgtctgaga
    22741 aaataagcgc ttcaatctct tttctcataa tcatcactcc aatgtttata tattatttat
    22801 ataaactctc tcgtctctct ctatttatta acttcttaca agactaatat taca
//
LOCUS       DBNENW010000028        22753 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNENW010000028 DBNENW010000000
VERSION     DBNENW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.028"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            107..1183
                     /locus_tag="AC1LW8_000872"
     CDS             107..1183
                     /locus_tag="AC1LW8_000872"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIP4772726.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1431..2093
                     /locus_tag="AC1LW8_000873"
     CDS             1431..2093
                     /locus_tag="AC1LW8_000873"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772727.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVHNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            2086..2862
                     /locus_tag="AC1LW8_000874"
     CDS             2086..2862
                     /locus_tag="AC1LW8_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772728.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAIVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            3258..4445
                     /locus_tag="AC1LW8_000875"
     CDS             3258..4445
                     /locus_tag="AC1LW8_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4772729.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4507..5859)
                     /locus_tag="AC1LW8_000876"
     CDS             complement(4507..5859)
                     /locus_tag="AC1LW8_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIP4772730.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(6166..7803)
                     /locus_tag="AC1LW8_000877"
     CDS             complement(6166..7803)
                     /locus_tag="AC1LW8_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIP4772731.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNTIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            7983..8582
                     /locus_tag="AC1LW8_000878"
     CDS             7983..8582
                     /locus_tag="AC1LW8_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIP4772732.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARSEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            8586..8726
                     /locus_tag="AC1LW8_000879"
     CDS             8586..8726
                     /locus_tag="AC1LW8_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772733.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCACHGQHFDVGIPLQA
                     WE"
     gene            8806..8925
                     /locus_tag="AC1LW8_000880"
     CDS             8806..8925
                     /locus_tag="AC1LW8_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772734.1"
                     /translation="MDVCPPVTMDACALLQKFEFCNNISHFRHFFAIKQPIER"
     gene            9252..10478
                     /locus_tag="AC1LW8_000881"
     CDS             9252..10478
                     /locus_tag="AC1LW8_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP4772735.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRSIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSDNHLLGFLDIEDINQGIRGHKTLRDMMQQHIYTVQIDSKLQDSVRTI
                     LKRNIRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            10475..11110
                     /locus_tag="AC1LW8_000882"
     CDS             10475..11110
                     /locus_tag="AC1LW8_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772736.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            11127..12068
                     /locus_tag="AC1LW8_000883"
     CDS             11127..12068
                     /locus_tag="AC1LW8_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772737.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            12068..12763
                     /locus_tag="AC1LW8_000884"
     CDS             12068..12763
                     /locus_tag="AC1LW8_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772738.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            13057..14457
                     /locus_tag="AC1LW8_000885"
     CDS             13057..14457
                     /locus_tag="AC1LW8_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4772739.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNTLLAIV
                     AFLVILLLVPKNQTNL"
     gene            14683..15618
                     /locus_tag="AC1LW8_000886"
     CDS             14683..15618
                     /locus_tag="AC1LW8_000886"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIP4772740.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQETVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            15940..16791
                     /locus_tag="AC1LW8_000887"
     CDS             15940..16791
                     /locus_tag="AC1LW8_000887"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIP4772741.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(16883..18379)
                     /locus_tag="AC1LW8_000888"
     CDS             complement(16883..18379)
                     /locus_tag="AC1LW8_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP4772742.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIHITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTQYITDHW
                     LKPLQMKHTTFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            18611..20440
                     /locus_tag="AC1LW8_000889"
     CDS             18611..20440
                     /locus_tag="AC1LW8_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIP4772743.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(20580..22658)
                     /locus_tag="AC1LW8_000890"
     CDS             complement(20580..22658)
                     /locus_tag="AC1LW8_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIP4772744.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
BASE COUNT         7496 a         3314 c         4383 g         7560 t
ORIGIN      
        1 aaattttaaa aatatttaag cctgaaaatg ataaagcggt agggaacgtt tttctgaaag
       61 ttagtgatac aatagtttaa agttgaaata caggaggatg aataacatga atcagtcagt
      121 caaattactt aaacatttaa cagatgtaaa cggcattgct ggttatgaaa tgcaagttaa
      181 agaagcaatg cgtaactata tagagcctgt cagtgatcaa attattgaag ataacttggg
      241 tggcattttt ggaaagaaaa atgctgagaa tggtcaatac tcaattatga tttctggtca
      301 tatggatgaa gttggtttta tggtaacaaa gattgataaa catggtttta tttcatttac
      361 gccagttggt ggatggtgga atcaagtcat gctatctcaa aaagtaacga ttacaacaga
      421 ttcgggcaaa gaaattagag gtatcatcgg ttctaaaccg ccacatgtct taacgcctga
      481 agaacgtaaa aagccaatgg aaatcaaaaa tatgtttata gatattggtg ttagtagcaa
      541 ggaagaagct gaagaagctg gcgttgaagt aggcaatatg gttacgccat atagtgaatt
      601 tgaagtgctt gcaaatgata aatatttaac tgcgaaagca tttgataatc gctatggctg
      661 tgcattagct attgaggtat taaaacgttt aaaagatgaa aatattggca ttaacttata
      721 cagtggtgcc acagtgcaag aagaagttgg tttgcgtggt gcgaaagtgg cagcgaatac
      781 gattaaacca gacttggcga tagctgtcga tgtaggtatt gcttatgata ccccaggtat
      841 gtcaggtcaa acgagcgata gtaaactagg cggtggtcca gttgtcatta tgatggatgc
      901 tacaagtatt gctcaccaag gtttgcgtaa gcatattaaa gatgtagcta aggaacataa
      961 catcgaagta caatgggata cgacaccagg tggaggtaca gatgcgggaa gtattcatgt
     1021 cgcaaatgaa ggtattccca cgatgacaat cggtgttacg ctgcgataca tgcattctaa
     1081 tgtttcagtg ctcaatgtag atgattatga aaattctatc cgtcttgtta ctgaaattgt
     1141 ccgttcatta aatgatgaaa gttataaaaa tatcatgtgg taacccaatc cacaaataat
     1201 aaagaatcct tttaatatgg taggttgtta aacaattgtc taattttaat tcttagtcat
     1261 tagacagtat ccatgttaat aggatttttt gtttttaatt taaatgctga aaatcaatta
     1321 tgcctaaatt ttgatattac aagaaaatga ttttttctta aatataattg cactaaaaac
     1381 caaaaaaacg ggaataatat acctgatata ttacatgagg agcggtgcaa atgttgttag
     1441 aaattaaaga tttagtgtat aaagcgagcg atagaatcat actagatcat atcagtctaa
     1501 aagtagataa aggcgagagt attgccatta taggtccatc aggtagtggt aaaagtacat
     1561 ttcaaaagca aatatgtaat ttgattagtc caactagtgg agaactttat tttaaaggta
     1621 aaccctataa tgattatgac ccggaagaat tgcgtcaacg aatcagttat ttgatgcagc
     1681 aaagtgactt gtttggtgaa acgattgaag ataacatgat attcccatca cttgcacgta
     1741 atgataaatt tgatagaaaa cgtgcaaagc aattaattaa agatgtcggt ttgggacatt
     1801 atcaattaag ttcggaagtg gaaaatatgt cgggtggtga gcggcaaaga attgctatag
     1861 cgcgccaact gatgtataca ccggatattc ttttattaga tgaatcgacc agtgcattag
     1921 acgttcataa taaagaaaag atagaaaata tcatttttaa gttagtggaa caggatgttg
     1981 ctatcatgtg gattacccat agtgatgatc aaagtatgag acattttcaa aagcgcataa
     2041 cgattgttga tggcaaaatt tctaaggttg aggagttgaa tcaacatgag taatacggca
     2101 cttggattga cggcattgct tttagtcatt ccgattatca tttcatataa agaaggttta
     2161 catattatta aagatttaat tgttgcgaca ctacgcgcaa ttgtgcaatt aatcattttg
     2221 ggatttttgc tgcattatat ttttaaaata aacgataaat ggctgcttat tttatgtgta
     2281 ttggtcatta ttattaatgc atcatggaat acaattagtc gagcatcacc agtgatgcat
     2341 catgtgtttt ggatatcatt tctagctatc ttcattggaa cggcattacc gcttgcaggt
     2401 actattgcga caggggccat tcaatttaca gccaatgaag ttatacctat tggcggcatg
     2461 cttgctaata atggcttgat tgcaattaat ttagcttacc agaatttaga tcgtgcattc
     2521 gtacaagatg gtactaatat tgaatctaaa ttatcacttg cagctacacc taaattggct
     2581 tctaaaggtg caatacgtga aagtattcgt ttagctatag tgccaacaat tgattcggtt
     2641 aaaacatatg ggcttgtgtc gattcctggt atgatgacag gcttaattat tggtggcgta
     2701 ccacctttac aagcgattaa atttcaattg ttagtcgtgt ttattcatac aactgcgacc
     2761 attatgtctg ctttgattgc gacatattta agctatggtc aatttttcaa tgcaagacat
     2821 caattagtag cacgaaatac tgatgttaag agtgaatcat gatagatttt actgcatcag
     2881 atttaggtat tagttttaat tggaaatgaa gtgacgcgca catatagtat cgctattcat
     2941 taacacagcg aaaatattca taaaggcacg catactttgt agtcagttat ctgttctgac
     3001 atataaagcg tgcgtgcttt ttgggagtca ttgttgaaac tgaagtaatt atacataatt
     3061 attaaatgac atacttgtgt taatttttca aatactgaaa aacaatttca ataattttcc
     3121 aattaagcac agaaaattaa agcaaaatat tatataatag aacggttata tataaaattg
     3181 tgttgcacac attttttaat aaatcgttat tctaagggaa atgaatatcg gaaattttgt
     3241 ttgaaaggag ttttaaaatg tcaatcatgc gactatttac attcatttta agtattttta
     3301 tcgtaggaat ggttgaaatg atggttgcag gaattatgaa cttgatgagt caggacttac
     3361 atgtatcaga agctgtcgtt ggtcaattag tgacaatgta cgctttaaca tttgcgatat
     3421 gtggacctat tctggttaaa ttaacgaacc gtttttcatc aaggcctgta ttattatgga
     3481 cattacttat atttatcatt ggtaatggca ttattgctgt agcgccaaat ttttcaatat
     3541 tagtagttgg tagaattatc tcatctgcag cagcagcact aattatcgta aaagtattag
     3601 ctattacagc gatgttatca gcacctaaaa atcgtggtaa aatgattgga cttgtctata
     3661 cagggtttag tggggccaat gtttttggtg taccaattgg aacggttatc ggcgatttag
     3721 taggttggcg ctatacattt ctattcttaa ttattgtgag tattattgtt ggcttcttga
     3781 tgatgatcta tttaccgaag gatcaggaaa tacaacgagg ccctgtgaat catgagacac
     3841 catctcatga aaatcatgtt acttcgaaaa tattaagacc tgctgaagta gcaaaatatt
     3901 taattattac gtttttagta ttgattgcaa actcagtgac attcgtcttt ataaatccac
     3961 ttattttatc taatggtcat gatatgtcat tcgtttcatt agcacttcta gtaaatggaa
     4021 tcgctggcgt tattggaaca tcattgggtg gtatattctc cgataaaatt acaagtaagc
     4081 gttggttaat gatttctgtt tctattttta tcgttatgat gttacttatg aatttaatct
     4141 tacctggttc aggtctattg ttagcaggac tatttatttg gaatatcatg caatggagta
     4201 ctaatccagc agtgcaaagc ggtgtgattc aacatgttga aggcgacaca agccaagtaa
     4261 tgagttggaa catgtctagt ttaaacgctg gtattggtgt tggcggcatt attggaggct
     4321 tggtcatgac acatgtttct gttcaagcta tcacgtatac gagtgccatc attggcgcat
     4381 taggattaat cgttgttttc acattgaaaa ataatcatta tgctaaaaca tttaaatcat
     4441 cataattctc atatgaaaag cacgcctgct atcaaattca ggtgtgcttt tttagatgcg
     4501 ataacgttat tgatatgtgc gataatagcg acgttcatta tgatacatcg gccaaggcat
     4561 tttaccgctt ttagcaaaat tagctaaatc attttgcatt tgtcgactta aaaatttaag
     4621 gtgagcagtt gttggatatt gatgtgctgc taatatttgt aagtttccga accaaaacac
     4681 catatctaga atatggtaag cactgcgata gtgtgcgcta gaagtatcat gccatgcaaa
     4741 ttcagcaagc catagttgtg cgtttgaatc ttgtatattg agttgttgta aaaattgctt
     4801 catcggctgt ttgaaataac aatgtgtaat cgctaaagat tgttgcttcg ccgtctgaac
     4861 atcttcgtat tttaaaggaa tatcatttaa ttccataatg tcgataaagc gttgcggcga
     4921 taattttttc tgttcactag taatataaat atcgccttca tcttttgtat aacatgcaaa
     4981 aattggtttc gttgttggat aattattttg tatataatct gttttaatag gcgcatatat
     5041 taaatcaagc cctttagaag gtcctcgaga ttgttttagc ttcgccatca gcataagaat
     5101 atcatctgtc gataatgatg taacatcatc tgtatctaaa taatcgagca tcaatttttg
     5161 gaaatattgt gctttattgc gtgcactctt aagggtgtct aatcgtagtg cgccacttag
     5221 tagaacgact ttatggaagt atggctcaat gtcaggtatt ttaagtaaag tcaaaatgct
     5281 catactgcct gcagactgac ccattaaagt aatgttatta gcgtcgccac cgaaggattc
     5341 aataaattga tgcacccatt ttatgacatt gatttgatct gaaaggccat tattggaatg
     5401 aaaaccttta ttaaaatatg accagtctaa atatcctaat gcgcctaaac gataattgca
     5461 tgtaataacg ataatgtcgt tattttgtac taaatgtgcc ggttgataga gttcggctgt
     5521 accatgacca ttttcaaaac taccaccata aaaataaatg atgacaggtt tcttcgtctg
     5581 atcattatgt tgtttccaaa tatttagata taaacagtct tcatgttcag taaaagttgt
     5641 agattgtgag gagaaaaaat cttctaattt gttgtctggt tgcggtggga tgggttgtat
     5701 ttcagttgca tcaattggct ctgaccattg tgtttttaac gtagaatgtt taaagcgatt
     5761 gtcatgaact ggtggttctg cataaggaat gcctaagaag atatctaaac catcttgtgt
     5821 aataccatga atttgaccac ctgtagtatt aatcttcata tttattaacc tcctttatca
     5881 tccccaattt aatcatacca cggtctatag atgataggtg acaagtataa caagacaatt
     5941 ttgtgactga atcagacgag tttgatatct gttttaactc aaaccatttt cagcattata
     6001 tgatgttctt ctacagtgac atttcattac aatatcactc tcgccatctt gttatgttag
     6061 ttatttataa ccgattttca aaataaaaaa ccaaccaaga tgttgaaatc aatggttggt
     6121 taatgtgtgt gttgaaatca tatatggcac tgtattttaa aatatttaat gagatgactt
     6181 tgattcatta tttagtgact cacgcatctt cgtattgatt cgtgttgcgc gacggaaata
     6241 gacgctttca aatgaactcg ttgtaccgat gtaatagaat ataagcgcca caacaataat
     6301 aacgataaag tcaaatggat agtgaatcat atttaagcct ttaaattctt tgcttcctat
     6361 aaatgacagt attgatagca cgattaaata tacaataatc cataagctac caccaatttg
     6421 tttctttgta ttacgccaat tcatacggta ttcatagaag aagtagattg gtaatccaag
     6481 tataatgatt aaaataactt cagccgttgt tggccacatt gcccaatata tagctaatga
     6541 agctaataca aatgataatg gtgccattac ttttaaaatt tttgctctaa atggacgagt
     6601 cattgttggt cccatttttc ttaatgcaat cactgtcgtt gggccagtta aataggctac
     6661 taaagttgca gtagaaataa ctgctgctag cgtaccccaa tctctaaata atgtaaccat
     6721 aatcatacta atgatggtat taaagatgat tgctacacgt gggatatgat atttttcatt
     6781 catcttccca agaaatttag ggatatgtcc atttttctcc attgctcgta aaactcgacc
     6841 tgtaacggcg acaaatgaca cgccagtacc gaatggtgat acaaaagctt caatatatag
     6901 taaaattgcg agccaattaa ttcctaataa gatagctaaa tcagcaaatg gtgaattgaa
     6961 gttgatgcca ttccatccac tatgttgtaa cattgattga ggcatagaag tgataaacgt
     7021 actttgtaaa atgatgtaca acactgcact gattgacagt gagatagcga tgcctcttgc
     7081 gatatttttt tcaggatttt taatttctga acccatatta ataattgtct ggaatgaatt
     7141 gaatgaaaaa ataatccctg atgctgttgt tgcagcaaaa atcggtgcac ttccgtaagg
     7201 cataaatgtg cttgccgaat ggccgtaatt tgaagtgtcg aatcctgata gcatcaacat
     7261 gatgatggtt aacatgggta cgcctaattt aaatacagaa attaaactcg taaatgatgt
     7321 taaaagtttt acggaccaat agtttaataa tgaaaaaata acaatgatga gatatacagc
     7381 tagcaatccg tatgtgctaa tagagccatt ttccattaaa tatctcattg gtttcgccca
     7441 atgccacggc caagaactca tatattgaac agctgacaca gcttcgatag gtattattgt
     7501 caccaaagac acccaattcg cccaagcagc aataaagcct aataatgagc catgtgtata
     7561 ctgggcatag ttactcatgc cacctgattg aggaaacatt gtgccgattt caatgtagtt
     7621 ataggcaatg gttccaatga ctaggaatcc aagaacccat gatatgattg ctgctggtcc
     7681 agctattgat gatgcttccc acgcaccaaa tagccaacca gatcctatta aagagcctaa
     7741 ccctaataag actaattggg atagattgat tttgtcgttg tgcaattgct tgtctttatt
     7801 cataaatact ccttcgttat tgccaaaaat acaaaaaata atatgcgaca attatacgct
     7861 tattataata atagtcaaat gcattttttg taagaacgca aaagcatttg taaaattagt
     7921 tttttataaa attaggatat ataatacaat gtgtattgaa aaacaaaatg gaagtgatga
     7981 ctatgagtga gaaacaatca aatgaacaag ttgaagcatt tttaaataaa gagagtcaat
     8041 ggcaagaaga atataaatat ttgagagcgt taatctttaa tgaaacagaa ttagaggaag
     8101 cgtataaatg gatgcatcct tgttacacgt tgaataataa aaatgtagta cttatccatg
     8161 gcttcaaaaa ttatgttgca ctattatttc ataaaggtgc cattttggag gataaatatc
     8221 atacactcat tcaacagact gaaaaggtgc aagcagctcg tcagttacga tttgaaaatt
     8281 taacagagat tcaagcacgt agcgaagaaa ttaaatatta tctagctgaa gcaattaaag
     8341 ctgaaaaagc tggtaaaaaa gttgaaatga agaaaacaga ggaatatgtt attcctaaag
     8401 agttagaagc taagttcgaa gaaatgccac agttagagtc ttcattttat aaattaacgc
     8461 caggcagaca acatcaatat atatatcata ttggacaagc taaacgcagt gaaacaagac
     8521 aaaagcgtgt tgaaaagtat attaaccaaa tactagaagg taaagggatg catgataagt
     8581 aattaatgag taaagcatac cggttataca acaacataca agatgacacg aaacaaccaa
     8641 tggctcatgc tgttggttgt ttttttaggt gtgcctgtca tgggcaacac tttgacgttg
     8701 gaattccgtt acaagcttgg gagtaggaaa tgttagcaaa aggcaagggt gtctacaatg
     8761 aatgatgaaa atattaaaat ataaggatga ctttgtgagt ggctgatgga cgtttgtccg
     8821 cctgtaacaa tggatgcgtg tgcattatta caaaaattcg agttttgcaa taatatttca
     8881 catttccgac acttttttgc tataaaacaa ccaattgagc gataataaat tcgcttttaa
     8941 aaaatatgag ttatctattt agttgccaaa gataaaataa taatgtttaa taacatcata
     9001 tagagtatgt tagttttaaa tgtcgaatat acgaatgtgc aaacaaagta atcggtagaa
     9061 attcaacata catagcgccg tttactgtta agtattcaca ttacagatga aaaatataaa
     9121 attctacata atcaagacca tgatgtgtac ttgtttaact tatgactcta tttgtttaac
     9181 aattgcgatg atggtctttt tattttatgc gtatcattcg tcatattttt tatgaggaag
     9241 gagaaatgat tatgttaagt attaagcatt taacgaaaat ttattctggt aataaaaagg
     9301 cagtagatga catctcttta gatattcaat ctggggaatt tatcgcattt attggaacca
     9361 gtggaagtgg taaaacgact gctttaagaa tgataaaccg tatgattgaa gcgacagaag
     9421 gacaaattga aattgatggt aaagatgttc ggagtatgaa tcctgtcgaa ttgcgtcgaa
     9481 gcattggtta tgttattcaa caaattgggt taatgcctca tatgacgatt aaagagaata
     9541 ttgtattggt acccaaattg ttgaaatgga ctaaagaaga aaaggataaa cgtgcaaagg
     9601 aattaattaa acttgtggat ttaccggagt catttttaga gcgttatcca gcagaactat
     9661 caggtgggca acaacaacgt atcggtgtcg taagagcact tgcggccgaa caagatatta
     9721 ttttaatgga tgaacctttt ggtgcattgg atcctattac gagagatacg ttacaagatt
     9781 tagttaaaac gttacaacga aaattaggca agacgtttat ctttgtaaca catgatatgg
     9841 atgaagcgat taaattagca gacaaaattt gtattatgtc agaaggtaag gtggtgcaat
     9901 ttgatacacc agacaatatt ttaagacatc ccgcaaatga ttttgtacgt gattttatag
     9961 gacaaaatag actgattcaa gaccgtccca atgacaagac tgtagaaggt gtaatgatta
    10021 aaccaatcac gatacaggca gaagcaacac tgaatgacgc cgttcatatt atgagacaaa
    10081 aacgtgttga tactattttt gtcgttgata gtgacaacca tttacttgga ttcttagaca
    10141 ttgaggatat taatcaaggt atacgaggac acaaaacttt gcgtgatatg atgcaacaac
    10201 atatttatac cgttcaaatt gattcgaaat tgcaagattc tgtacgcacg attttaaaaa
    10261 gaaatatcag aaatgttcct gttgtagatg atcaacagcg tttagtagga ctgattacgc
    10321 gtgccaatgt tgttgatatt gtatatgaca cgatttgggg cgatagtgag gatacagtgc
    10381 aagcagaaca tgtgggggaa gacactgcgt cctcaaaagt gcatgagcaa cacactacta
    10441 atgtcaaagt acgtgacata ggagatgata aatcatgatt gagttcctac atgaacatgg
    10501 tggacagttg atgtcgaaaa cactggaaca tttttatatt tctatagtgg cattattact
    10561 tgccatcatt gttgcagtac ctataggcat tttattatca aaaacaaagc gaactgccaa
    10621 tattatatta actgtggcag gtgtcttaca aactattcca acactagctg tacttgctat
    10681 tatgataccg atttttggtg ttggtaaaac gcctgcaatt gtagcgctat ttatttatgt
    10741 attattacct attttaaata acacggtact cggtgttcaa aatattgata gcaacattaa
    10801 agaagctgga aaaagtatgg gaatgacaca atttcaattg atgaaggatg ttgaattgcc
    10861 gttagcatta ccgcttatca ttggtggcat tcgtttgtcg tctgtgtatg taattagttg
    10921 ggctacactt gcaagttatg taggtgcggg tggattaggt gatttcattt tcaatggttt
    10981 aaatttatat gatccactga tgattgtaac tgcaacggta ctcgttactg cactagcatt
    11041 aggtgttgat gccttattag ctttagttga aaaatgggta gttcccaaag gcttaaaagt
    11101 atctggataa ttaggaggct aagataatga agaaaattaa atatatactt gtcgtgtttg
    11161 tcttatcgct taccgtatta tctggatgta gtttgcccgg actaggtagt aagagcacga
    11221 aaaatgatgt caaaattaca gcattatcaa caagcgaatc gcaaattatt tcacatatgt
    11281 tacggttgtt aatagagcat gatacacatg gtaagataaa gccaacatta gtaaataatt
    11341 tagggtcaag tacgattcaa cataatgcct taattaatgg ggatgctaat atatcaggtg
    11401 ttagatataa tggcacagat ttaacgggag ctttgaagga agcaccaatt aaagatccta
    11461 agaaagcaat gatagccaca caacaaggat ttaaaaagaa atttgatcaa acgttttttg
    11521 attcgtatgg ttttgcgaat acgtatgcat tcatggtaac gaaggaaact gctaaaaaat
    11581 atcatttaga gacagtttca gatttagcaa agcatagtaa agatttacgt ttaggtatgg
    11641 atagttcatg gatgaatcgt aaaggcgatg gctatgaagg atttaaaaaa gagtatggtt
    11701 tcgactttgg tacagtgaga ccaatgcaaa taggtctagt ctacgacgca ttaaactcag
    11761 agaagttaga cgttgcatta ggttattcta cagatggtcg aattgccgcg tatgatttga
    11821 aagtacttaa agatgataaa caatttttcc caccttatgc tgcgagtgct gttgcaacaa
    11881 atgaattatt acgccaacac ccagaactta aaacgacgat taataagttg acaggaaaga
    11941 tttcaacttc agagatgcag cgcttgaatt atgaagcgga tggtaaaggt aaagaacctg
    12001 ctgtcgtcgc agaagaattt ttgaaaaaac accactattt tgataaacag aaaggtggtc
    12061 ataagtaatg gaaggtaatt tattacagca attattcaat tattatgtta cgaactttgg
    12121 ctatctatgg gatttatttt tcaaacactt attaatgtct gtctatggtg tgctgtttgc
    12181 agctttaatt ggtattccat tgggaatctt gcttgcaaga tacacaaaac tttctggatt
    12241 tgtaattaca attgcaaata taattcaaac agttccagtc attgcaatgt tagctatttt
    12301 aatgttagtc atgggcttag gttcagaaac agtagtttta acagtgtttt tatatgcgtt
    12361 acttccaatt ataaaaaaca cttatactgg tatagctagt gttgatgcga atattaagga
    12421 tgctggcaaa ggtatgggaa tgacacgcaa tcaagtgcta cgaatgattg aattaccgtt
    12481 atctgtttcg gttattatcg gtggcattcg tattgccttg gttgttgcga taggtgttgt
    12541 tgccgttgga tcatttatag gagcacctac gcttggtgac attgtgattc gtggtacaaa
    12601 tgcgacggat ggcacaacgt ttattttagc aggtgcgatt ccgattgcta tcattgcaat
    12661 cgtcattgat gtactattaa gatttttaga aaaacgatta gacccaacaa cacgacatcg
    12721 taaaaatcaa tctaatcatc ggccgcaaag tattaatatg taatagtaga agatgtttat
    12781 aatttagcga tttcgtttca tgatttataa aaaatgaggc tactcaagga gctcaaataa
    12841 tctttgagta gcctttttat aggttgtgtt tgtatgcgtt tacactaaaa tagcaattat
    12901 tatcatgaaa gtttttggat aaaaagcgtt aattattgta aaaatgctaa aaaatgagat
    12961 gttttattta taattttctg caaatttatg atattgtttc ttaatatatc atattaaaaa
    13021 tttgtttttc ttaaacatag gaggcttatc taattcatgg acacatcaaa acaatttaga
    13081 ggtgacaacc gattgctttt gggtatcgtt ttaggggtta ttaccttttg gctattcgcg
    13141 cagtcacttg ttaatcttgt tgtcccatta caatcaacat atagtagtga cgttggaacg
    13201 ataaatatcg ctgttagctt atctgcctta tttgctggtt tgtttatcgt aggtgctggt
    13261 gatgttgctg ataaatttgg tcgcgtcaaa attacttatg taggattgat attaaatgtt
    13321 gtaggttcat tactcatcat cattacacct ttgccagcat ttttaattat aggtagaata
    13381 attcaaggtt tgtctgcagc atgtattatg ccatcaacac ttgctattat taacgaatat
    13441 tatattggta caagaagaca acgtgcctta agctattggt ctattggttc ttggggtggt
    13501 agtggtattt gtacgttgtt tggtggctta atggctacat atataggttg gcgttcaata
    13561 tttgttgttt caattctatt aacattatta gcaatgtact taatcaaaca tgcacctgag
    13621 actaaagcag aaccaatcaa aggtatgaaa gcagaagcta aaaagtttga cgttattggt
    13681 ttagtcattt tagtagtgac gatgttaagt ttaaatgtaa tcatcacaca gacgtctcat
    13741 tttggtttag tttcaccgtt aattctaggt ttaattgttg tgtttatctg ttcattagtt
    13801 ggttttgtat attacgaaaa taaaataaag catccacttg ttgatttttc aatttttaaa
    13861 aatagaggat acagtggtgc aactatttca aacttcttat taaatggtgt agcaggtggt
    13921 gcacttatcg tcattaacac gtattatcaa caacaattag gatttaattc ttcgcaaacg
    13981 ggttatattt cattaacgta tttaatagca gtgttgtcaa tgattcgtgt aggtgaaaag
    14041 attttatctc aacatggtcc gaagcgccca ctattactag gaagtggctt tacagtgatt
    14101 gggttaatct tattgtcgtt aacattttta ccagaaatgt ggtatatcgt atctagtata
    14161 gttggatatt tattgtttgg tactggttta ggattatatg ctacaccatc aactgataca
    14221 gcagttgcta gtgcgccaga tgataagtcg ggtgttgctt caggtgtgta taaaatggcg
    14281 tcatcattag gaaatgcatt tggagtagca gtatctggta cggtttatac tgtgttagca
    14341 gctaatttaa atttgaactt aggtggtttc acaggtatga tgtttaatac cttgctagca
    14401 attgttgcat ttttagtcat tttactatta gttcctaaaa atcaaacgaa tttgtaaaac
    14461 tgaaatgaaa gcaagttatt atgtacggat tttaaaggaa attttgtgaa agtaagttta
    14521 tcatacacac ttaatgttac gtattgacgt ttaatgttag gtgtgttctt ttatagacga
    14581 taaaagctgt gtgcatatta agcgaatgat tttcaaattg acgctaatat gcgaaagtag
    14641 tatttttaaa atgaacaaca acgatgaaga ggggtttata ggatgaaaat tgcaattgct
    14701 ggatcgggtg cattaggtag tggctttggt gccaaactat ttcaagcagg atatgatgtc
    14761 acacttattg acggatatac atctcatgtt gaagcggtta agcaacatgg attaaatata
    14821 acgattaatg gagaggcatt cgagttaaac attccgatgt atcattttaa tgatcaaccg
    14881 gacgaaagca tttacgatgt tgtctttcta tttccaaagt ctatgcaatt aaaagaagtg
    14941 atggaagata tgaagccaca tattgataat gaaacgatcg tcgtatgtac gatgaatggt
    15001 ctgaagcatg aagaagtcat tgcgcagtat gttgctcaat cacaaattgt cagaggtgtt
    15061 acgacttgga cggcaggtct tgaaagccct ggacacagtc atttacttgg tagtggacca
    15121 gttgaaatag gtgaactagt ggatgaaggt aaagaaaatg ttataaaagt tgctgattta
    15181 cttaacgaag cggaattgaa tggtgtcatt agtaaagatt tataccaatc gatttggaaa
    15241 aagatttgtg ttaatggtac ggcaaatgca ttaagcacag tgttggagtg taatatggca
    15301 tcgctgaatg aaagtagtta tgcgaagtgt ttgatttata aattaacgca agaaacagtg
    15361 catgtagcga cgattgataa tgttcattta aatgttgatg aagtatttga atatttagtt
    15421 gatttaaatg aaaaagttgg tgcgcattat ccatccatgt atcaagattt aattgttaat
    15481 aatagaaaaa ctgaaattga ttatattaat ggcgcagttg caacattggg taaacaacgt
    15541 catattgaag cgccagtcaa tcgctttatt actgatttaa ttcatactaa agaaagtcag
    15601 cgacacgcac aggattaatt ggtgttttag gtatgattac aaggaaatga caatcacgtg
    15661 atattacggt cattattaag attgaaatgt aataaataaa gaacagcagt aaggtacttt
    15721 caaattgaaa tgatcttggt gctgtttttc ttgattgatt ttcgtcataa ttcagatttg
    15781 tcataggtct acgacatact attagtattt actagacagt ttttacgacg acactttgaa
    15841 aaattttgag gcaaatcatt tggaagtctc acgtgaattt tgtaaactca tcaagcaagt
    15901 aattatatta aaaagacaaa tagagaaaag gtgtttataa tgagtaaaat ttttgtaact
    15961 ggtgcaacgg gccttattgg cattaaatta gttcaaagac taaaagaaga ggggcatgag
    16021 gttgctggtt ttactacatc tgagaatggt caacaaaagc tagctgctgt taatgtaaaa
    16081 gcatatattg gtgatatatt aaaagctgat actattgatc aagcgttagc agattttaaa
    16141 ccagaaatca ttatcaacca aattacggat ttaaaaaatg ttgatatggc agcaaatacg
    16201 aaagtacgta ttgaaggttc taaaaactta attgatgcgg cgaaaaagca tgacgttaag
    16261 aaagtaattg cccaaagtat tgcctttatg tatgaacctg gcgaaggatt agcaaatgag
    16321 gaaacttcac ttgattttaa ctcaactggt gatagaaaag taacggttga tggtgtggtt
    16381 ggtctagaag aagaaacggc tcgtatggat gaatacgttg ttttacgttt tggttggtta
    16441 tatggcccag gtacttggta cggaaaagat ggcatgattt ataatcaatt tatggatggt
    16501 caagtgacac tttcagatgg cgtaacatca tttgtgcatc ttgatgatgc agttgaaaca
    16561 tctattcaag ctattcattt tgaaaatggt atctataatg tagcagatga tgcacctgtt
    16621 aaaggttctg aatttgcaga atggtataaa gaacaacttg gtgttgaacc aaatattgat
    16681 attcaacctg cgcaaccatt tgaacgtggc gtaagcaatg agaagtttaa agcgcaaggt
    16741 ggtactctga tttataaaac ttggaaagac ggcatgaatc caattaaata ataatttatc
    16801 cgtttaatat acaaagaata aagacttggt cgaatcgtgg atgatatatt atcaaacgca
    16861 cggctcgaac aagtcttttt tattatgtct tcgttatctt tgtatgaagg aataacagaa
    16921 ttacaattaa tgtactgaat aatgcaatta atgttgtgat tagtgctaat ttaatttcta
    16981 ttggtagcca agtcagtaca aaagaccaat tattgctacc gagaatgaga tatggtaatg
    17041 catataatat gagcgctata gcgatacata aacataaagt tagccaactc aatacagcaa
    17101 tccgttttga tctacgcaaa agaatttgac catggcgcat ttgccaagca cgataagctg
    17161 agaatataaa tgctaataaa ataagtgttg tcatcaaaac ggtaacaata ttaaattggt
    17221 ctttatattg attgagtata gacgcaaccg tcgaatatcg cttgtgattt acaatttgtg
    17281 tattaagatg ctcaactaat ttgggtacat attccgaatt tagatttgca agcacaacaa
    17341 ttccataatt ttgttttgga tttagtaaaa taaatgatga aaagttatcg agcgttcctg
    17401 aatgaaacac taaatgttca tcattattgg taaaccagcc cgaagcatat gcattggcat
    17461 taggttcacc aattgttgat gataaatttt tatgtgattg ttgaactaat gatttgtatt
    17521 tatcaggtgg attaagttgg aattttatcc aatgttccaa atcttcagtt gatgtcatca
    17581 tatatgctga tggtgtatcc caaaggttaa attcaggttt agagacgaca ggtgtcgaac
    17641 cttgtaattc gtagccaata gcatcatgtt ttgatttgta atttgtttgt ttaaatgtcg
    17701 tgtgtttcat ttgaagtggt ttaagccaat ggtctgttat atattgcgta tatgattgct
    17761 ttgtgacatt ttggataatt aaacctaata aatcatagtt catatttgag tattcaaatt
    17821 cttctccggg cttatgatgt aattcatcac ccataattgc acgggttaca tcatttaaac
    17881 gattattttt atttgttacg gcgtcttcgc ttgtaatatc actaggtata ccacttgttt
    17941 gagccaaaag ttgcttaatc gtaatagttt cattttgacc attatagttc attttaaaat
    18001 gaggcacatg tttggatacg gcatcattta agtttaatcg accttcttga gctaatttta
    18061 aaattgcaag acctgtgaaa gctttcgtat tagaagcaat ttcatacttt gttgtgggag
    18121 aagctttgac ttttttatca acatcttgat aaccataacc tttatttaaa aaaactttgc
    18181 cattttttac tattaaaatt gatgctccag gaatgtgtcc cttttgtaaa tcatgctcga
    18241 taattgtatc tatttgttgt tgcgaatcat tggttaaccg tgtcttcgta ttgctattta
    18301 atgttatatg tatagcaatt gaaatggcga ctaaaatgat aatagaaatg gatagaaaat
    18361 acagtttttt agtagtcata aacgttcctt tttatatgtt aaatttactt aagtgcctaa
    18421 tgagatttat tttattatac cagttctatt gaataaatta attaaagagg tatatttacc
    18481 attatttttg acaccttata tgttaatagg tgtataatta cataaaaaat attaattaac
    18541 tctctatact ttttggaaag ataggcgtat agtgatatcc atcaaaaact ataaaaagga
    18601 ataggagatt atgttcaatc aatttaaaag acttattata gggcaaccta aaaaaaacag
    18661 agaactaaaa gacgaaaaga tatcgaagtt taaagggtta gcgattcttt cgtcggatgc
    18721 attatcttca gtggcttacg gtcccgaaca aatactgatt acattatcag tagtgggtgc
    18781 agttgcaact tggtatactt taccgattgc aggtgctgtt ctgatcttat tagctgcttt
    18841 aattatgtct tataggcaaa ttatttatgc atatcctaaa ggtggcggcg cgtatatggt
    18901 gtccaagacc aatttaggag aaaaatgggg attgcttgca ggaggctcat tattagtcga
    18961 ttacatactg actgtcgctg ttagtatatc atctggtgct gatgcatttg ttgctgcatt
    19021 cccaagttta tatgggcata aagtactgat tgcatgttta cttgtacttt ttattttaat
    19081 attaaattta cgtggtttaa ctgaatctgc caccgtatta tcctatccag tatatctttt
    19141 cattataggg ttagtgatat taatatttat aggtactttc cgtgtggcga caggagatat
    19201 tcaaccacat atgcatgcat cagtaggaac tgcggttcct ggagtaacat tattcttgct
    19261 attaaaagca ttttcatcag gggcttcatc attaacaggt gttgaagcta tttcaaatgc
    19321 ggtaaccaat tttagagaac caagtgctaa taatgctgta aaaacgttaa ttgctatggg
    19381 ttcaatttta gcgtttttat tagttggtat cgttggttta gcatatgttt atggaattat
    19441 gccacaaacg gaaacgacag ttttatcaca attagctatg caaatatttg gtgataatgc
    19501 tgccttctat tttgtacagg caacgaccgt gatgattttg gtattagcag ccaacactgg
    19561 atttacagct tttccgatgt tagcggcttc aatgtctaaa gataaatata tgcctagaat
    19621 gtttactgtt agaggtgatc gcttaggcta ctctaactcg attattattt taggtgtatt
    19681 agccattatt ttaattattg tatttgatgg tatgactgaa gatttaattc cattgtatgc
    19741 ggttggtgta ttcattccat ttacactagc acaattcggt atggttatta aatggattca
    19801 tgaacgtcct aagaattggt taagtaagtt gtctgtgaac ttactaggtg gtatcgtgac
    19861 atttattgtt tttatgattt tactcataac taaatttagc caagtatggc cgatacttat
    19921 tttcttgccg ttcgtggtga ttttcttctt gaaaattaac aaacattatc gagatatcgc
    19981 agaacaatta cgttctgaca ttgatgttct taatgtggac gtggtagatc gtaatttagc
    20041 aattgtgccg ataacaagta ttacaacagc agttgataag tctatttatt acgcacaaat
    20101 gcttgcgaat aatgatgtca tagcagtaca tgtatcattt ggagatgaag atgaaaaagc
    20161 attccaagag aaatggaaac gtcatttccc agatgtaaga ttagttattt tacattcgga
    20221 atatcgcagt attatacgac caatttcacg ttttatcgat aagattaatc gtaaagcaaa
    20281 cgatcaaaac tatatgatta cagtagtgat accagaattt attactaaga aacgttggca
    20341 taatttatta cacaatcaaa ctagtttgcg tatgaaattg tatttaattt atcaaaagaa
    20401 tgttaacgta tgtacaattc catttaaact taaaaaatag attctataat caaatcggat
    20461 tgatgaaagg ttatttactt ttcatcaatc cgattttttg atgttgaata taaaaacccc
    20521 gtacatatga gatgataatg actttgtctc atatgtacgg gggtattgtt atgtcttgct
    20581 taatgtttgg actgtaaaga caagacaatt tcgttgtaat tgatattttc tcgaatttga
    20641 gctgcaacga tattgttgat tttatttttg cgaattaatt catctaaaag atgacgttgt
    20701 aagtacaatg cttccaattt gtggttatgg aacatagctt gcggtatgat ttgattgctg
    20761 ttttcttgac gttcttttcg acgttgtgca cgccacttta atgagcgact catatcatag
    20821 taatgattaa tgactaagct aacctcaagt acatttgtac tattttgttc ttcttttaag
    20881 cgtaaaataa tattgtgatt cacaatacgc ataatttttt gaacatcttt gaatgaattt
    20941 tcaaattctt gcttcttaga ttctttattt aaggctttgt agtgcttcat cgctttaagt
    21001 ctaagccata acatcttgaa gaagtttgca aatttttcaa caaatgatgc agtacggtgt
    21061 gtctctgtta attcgacaat attgcggtaa ttattaatat cctgatatgt tgccttacct
    21121 ttatcaatta aacgctcaag tgtagatgtt tcgattactt ttgcaatatc ttctaaacgt
    21181 ttgagttctt ttgtattttg attatcaggt tctgaattta ataaaaataa caattctcca
    21241 tagtattggt ttaatactgg gcgataattt gtatcgtttt tgtctttttt actttcgttt
    21301 ttaaaatgct ggatcacttt ttgaacaatg aaaattttgg cagattgata agtcatacct
    21361 ttaaaagtag tatcttcggc agatggtgta attaaaggta aaacaatttg cgctaagatt
    21421 aaactaatta ataccatgaa agatgcaata aacaataaat cattacggta ttcgaatgct
    21481 tgtccttttg taatgataaa tggtaatgta agtgccattg aaagtgaaat tgtaccgtga
    21541 ataccacaca tggtcataat aaatgcgtaa cgcacacgag aaggtggtgt ttcatgtgaa
    21601 tcatgttcct cgtctagata agattgtata tttttcggga aataaaaatc tttgtaccag
    21661 aaataaaccc aaacaaatcg acatgcataa attgcaatag cgattaatat agtgatcact
    21721 attaaaaatg aaaagttgtc aggctctgtt tggaaaatat ctatgactac agtaggaatc
    21781 ataaaaccta atacaacaaa tacaaaacca tttaaggcat aactaaatgt gttccaaatt
    21841 tgatggtaat tcatttgtag ttccgtttgt gctctaatta aacggtcgcg ttctaaacca
    21901 tggattagac ctgcgattac aactgcaatg atacctgaag catgaacttc ttctgctaaa
    21961 aagtatacga caaaaggagt taataattga ataaaagtta aggtattgtt atcttttaaa
    22021 cctttattag cggttaagtc tatacgtatt ctaacgacaa cgaatccaat aattgcacca
    22081 ataagtacac ctagtattgt tgaaatgata aattgttcaa cagcttggaa taatgaaaag
    22141 gtaccagtta ctaatgcagt aacagcaatt ttaaatgaaa tgatacctgc tgcatcattg
    22201 agtaaagatt caccttctaa aatcgtcata gaacctttag gtaataattt tccgcgtgta
    22261 atagcagata ctgctactgc atctgtagga cataaaattg ctgctattgc aaaagcggct
    22321 ggcattggta aggcaggcca aatccaatgt ataaaatagc caacaccgac tacagttgca
    22381 aacactagtg ccattgacat taatagtata ggtttacgat attctaataa ttttgttcga
    22441 gagacgtggg taccttccac aaaaagtagt ggcgcgataa cggcaaacat aaatacttca
    22501 gaattgaatt ggaaatcaac ttgtattgga ataatgaaaa taacgacacc taatgcaatt
    22561 tgaataaagg cagtaggaat ttgcgggaat cgattattga taaccgaact aataatcaca
    22621 gcaaaaataa aaattaaaaa tgcttctaat agtgccatac aatactcctc aaaattttaa
    22681 tagttaatat tttatcactt ttaaggcata atgacataga tatattgata aaatgaagtt
    22741 attttcaaaa aaa
//
LOCUS       DBNENW010000029        22314 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNENW010000029 DBNENW010000000
VERSION     DBNENW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.029"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(8..634)
                     /locus_tag="AC1LW8_000891"
     CDS             complement(8..634)
                     /locus_tag="AC1LW8_000891"
                     /EC_number="1.6.5.-"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HIP4772745.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATIF"
     gene            843..1421
                     /locus_tag="AC1LW8_000892"
     CDS             843..1421
                     /locus_tag="AC1LW8_000892"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIP4772746.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYYL"
     gene            1804..2901
                     /locus_tag="AC1LW8_000893"
     CDS             1804..2901
                     /locus_tag="AC1LW8_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772747.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            2914..4185
                     /locus_tag="AC1LW8_000894"
     CDS             2914..4185
                     /locus_tag="AC1LW8_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP4772748.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            4188..5456
                     /locus_tag="AC1LW8_000895"
     CDS             4188..5456
                     /locus_tag="AC1LW8_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIP4772749.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            5458..6297
                     /locus_tag="AC1LW8_000896"
     CDS             5458..6297
                     /locus_tag="AC1LW8_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIP4772750.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            6371..7447
                     /locus_tag="AC1LW8_000897"
     CDS             6371..7447
                     /locus_tag="AC1LW8_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIP4772751.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            7472..8512
                     /locus_tag="AC1LW8_000898"
     CDS             7472..8512
                     /locus_tag="AC1LW8_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIP4772752.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            8567..9535
                     /locus_tag="AC1LW8_000899"
     CDS             8567..9535
                     /locus_tag="AC1LW8_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIP4772753.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(9896..10390)
                     /locus_tag="AC1LW8_000900"
     CDS             complement(9896..10390)
                     /locus_tag="AC1LW8_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HIP4772754.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            10623..12002
                     /gene="uhpT"
                     /locus_tag="AC1LW8_000901"
     CDS             10623..12002
                     /gene="uhpT"
                     /locus_tag="AC1LW8_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HIP4772755.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(12361..13119)
                     /locus_tag="AC1LW8_000902"
     CDS             complement(12361..13119)
                     /locus_tag="AC1LW8_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP4772756.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(13112..14668)
                     /locus_tag="AC1LW8_000903"
     CDS             complement(13112..14668)
                     /locus_tag="AC1LW8_000903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP4772757.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRGLLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(14665..15633)
                     /locus_tag="AC1LW8_000904"
     CDS             complement(14665..15633)
                     /locus_tag="AC1LW8_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4772758.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            16221..18470
                     /gene="pflB"
                     /locus_tag="AC1LW8_000905"
     CDS             16221..18470
                     /gene="pflB"
                     /locus_tag="AC1LW8_000905"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIP4772759.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            18493..19248
                     /gene="pflA"
                     /locus_tag="AC1LW8_000906"
     CDS             18493..19248
                     /gene="pflA"
                     /locus_tag="AC1LW8_000906"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIP4772760.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            19584..21347
                     /locus_tag="AC1LW8_000907"
     CDS             19584..21347
                     /locus_tag="AC1LW8_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HIP4772761.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(21511..21855)
                     /locus_tag="AC1LW8_000908"
     CDS             complement(21511..21855)
                     /locus_tag="AC1LW8_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HIP4772762.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            22045..>22314
                     /locus_tag="AC1LW8_000909"
     CDS             22045..>22314
                     /locus_tag="AC1LW8_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase domain-containing protein"
                     /protein_id="HIP4772763.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GKQIADGYYWGIIENLENQFYNIFHLLDQHKYAEKEYKDAVDKLKT"
BASE COUNT         7487 a         3390 c         4221 g         7216 t
ORIGIN      
        1 taaaaattta aaaaattgtt gctaattttt cagcattatt aatactagtt gctttaattt
       61 cttcagtctt atgaggttca gcattgtgtc cttcaataat gattgtttca tatgatggca
      121 cacctaagaa tgtcataatt gttcttaaat aacggtcacc catttcaaaa tcagcagcag
      181 gtccttcagt ataatatcca ccacgtgatt gaatgtgtaa tacttttttg tcagttagta
      241 aaccttgtgg tccttcagca gaatatttaa aagttttacc tgcaattgaa atagcatcaa
      301 tatatgcttt aactacaggt gggaaagaaa ggttccacat aggcgttaca aatacatatt
      361 tatctgcact taaaaattct tctaaaatgt cactcaatct tgaaactttc atttgttcat
      421 catcagttaa cgtttcgcca ttactcattt ttccccaacc agttaataca tctttgtcaa
      481 taactggaat ataagtttca aataaatcaa tatgtttcac ttcatcatca ggatgttgtt
      541 gttgatatgt ttcgataaat gctttaccag ccgccataga atttgatacc agttcattaa
      601 aagggtgtgc tgtaatatat aatacttttg ccatttgaaa attctcctct gtttctgtta
      661 ttttcttgag tataattatt atactagata taaaatttaa tatcaataaa aatattcaaa
      721 ttaccatcat tttcttcatc tatatttggc agtactacta aagtatgagt gcatttaatt
      781 atgaaatagt tgatttagaa tatatactta atacccaaaa tatatgaagg atggatgcca
      841 ctatgacaaa gcgaccaaaa cgtattttgg caacaattat catttttctt tcactattat
      901 ttacgattat ttatatagat gacattcaaa aatggtttaa ccaatatacc gataaattga
      961 cacaaaatca taaaggacaa ggacactcaa aatgggaaga cttttttaga gggagtcgga
     1021 ttactgagac ttttggtaaa tatcaacatt caccatttga tggtaagcat tatggcattg
     1081 attttgcatt gccaaaaggt acaccaatta aagcgccgac gaatggtaaa gtaacacgta
     1141 tctttaataa tgaattgggc ggcaaggtat tacagattgc cgaagacaat ggagaatatc
     1201 accagtggta tctacactta gacaaatata atgtcaaagt aggtgatcga gtcaaagcag
     1261 gtgatattat tgcatattca ggcaatacag gtaaacaaac gacaggcgca catttacatt
     1321 ttcaaagaat gaagggtggc gtaggtaatg catatgcaga agatccaaaa ccgtttatcg
     1381 atcagttacc tgatggggaa cgtagcctat attatttgta gttatagaag ggtgcccgca
     1441 gtctaaaaaa ttaagcaatc attgtgtgag tatgatactt acataatggt tgcttttttc
     1501 aatgaaaatc gtaatgctaa gtcatacttg tttgatttag atattactta aaatgtaaga
     1561 caaggttgtt agcattggca gtgaaatatc gcacataaaa aacattattg tcacactaga
     1621 aaatagttgt gcactatatc aattttctgt ataaaagttt aattctgaca gtaatgtaaa
     1681 cgtttacaat ttatgattga cattaataat gactgaatat atgatttatg taagtatttg
     1741 tgcaaacgtt ttcacaaagt gtattgcaca atcaaactgt aaacaaagta tgggaggcat
     1801 aacatggcag aactaaagtt agagcatatt aaaaagacgt atgataacaa caatactgta
     1861 gtgaaagatt ttaatctaca tattactgac aaagaattca ttgtatttgt tggaccatcg
     1921 ggatgtggta aatcaacaac attacgaatg gttgctggac tagagtctat cacatctgga
     1981 gatttttata ttgatgggga acgcatgaac gatgttgaac caaagaatag agatattgcg
     2041 atggtatttc aaaactatgc attatatcca catatgactg tttttgaaaa tatggcattt
     2101 gggctaaagc tacgtaaagt aaataaaaaa gagattgaac aaaaagttaa tgaagcagct
     2161 gaaatattag gattaactga gtatcttggt cgtaaaccaa aagcgttatc tggcggacag
     2221 cgtcaacgtg ttgctttggg cagagctatt gttagggatg cgaaagtctt tttaatggat
     2281 gaaccattat cgaatcttga tgcgaagctt cgagtacaaa tgcgcacaga aatattgaaa
     2341 ttacataagc gacttaatac tacgacaatt tatgttacac atgatcaaac tgaagcattg
     2401 acgatggcta gtcgaattgt tgttttgaaa gatggcgaca ttatgcaagt cggcacacct
     2461 agagaaatat atgatgcccc taattgcata tttgtggcgc aatttatcgg ctcaccagca
     2521 atgaatatgt tgaatgctac agttgaaatg gacggattga aggtaggaac acaccatttt
     2581 aaattacata ataaaaaatt tgaaaagtta aaagctgctg gctacttaga caaggaaatt
     2641 attttaggta ttcgagctga agacattcat gaagaaccaa tatttattca aacttctcca
     2701 gagacacaat ttgaatctga agtagttgta tccgaactgt taggttcaga aattatggta
     2761 catagcacat tccaaggaat ggaattgatt tctaaattag attcaagaac tcaagtgatg
     2821 gcgaacgaca agattacact agcatttgat atgaataagt gtcacttttt tgatgaaaaa
     2881 acaggaaatc gtatcgtcta agggggagta ttcatgtcta aaattttaaa atgtatcacg
     2941 ttagccgtgg taatgttatt aatcgtaact gcatgtggcc ctaatcgttc gaaagaagat
     3001 attgataaag cattgaataa agataattct aaagacaagc ctaaccaact tacgatgtgg
     3061 gtggatggcg acaagcaaat ggcgttttat aaaaaaatta cggatcaata tactaaaaaa
     3121 actggcatca aagtaaagct tgtaaatatt ggtcaaaatg atcaactaga aaatatttcg
     3181 ctagacgctc ctgcaggaaa aggtccagat atctttttct tagcacatga taatactgga
     3241 agtgcctatc tacaaggctt agctgctgaa atcaaattat caaaagatga gttgaaaggt
     3301 ttcaataagc aagcacttaa agcgatgaat tatgacaata agcaactagc attgccagct
     3361 atcgttgaaa caaccgcact tttttataat aaaaaattag tgaaaaatgc accgcaaacg
     3421 ttagaagaag ttgaagctaa tgctgccaaa ctaactgata gtaaaaagaa acaatacggt
     3481 atgttatttg atgctaaaaa tttctatttt aattatccgt ttttattcgg caatgatgat
     3541 tatattttca agaaaaatgg cagtgaatat gatattcatc agctaggact aaattcaaaa
     3601 catgtcgtca agaatgctga acgattacaa aaatggtacg acaaagggta tcttcctaag
     3661 gcagcaacac atgatgtcat gattggtctt tttaaagaag gaaaagtagg acaatttgtc
     3721 actggaccgt ggaacattaa tgaatatcaa gaaacgtttg gtaaagattt aggagtaaca
     3781 acattaccta cagatggtgg caaacctatg aaaccatttc taggtgtacg tggttggtat
     3841 ttatctgaat atagtaaaca taagtattgg gctaaagatt taatgctgta tatcactagt
     3901 aaagatacat tacaaaaata tacagatgaa atgagcgaaa ttactggacg tgttgacgtg
     3961 aaatcatcta atccaaattt aaaagtgttt gaaaagcaag cacgtcatgc tgaaccgatg
     4021 cctaatattc ctgaaatgcg acaagtttgg gaaccgatgg gcaatgcaag catatttatt
     4081 tcaaatggta agaatcctaa acaagcgtta gatgaggcga cgaatgatat aacgcaaaat
     4141 attaagattc ttcatccatc acaaaatgat aagaaaggag attagttatg acgaaacgta
     4201 accctaaatt agcggcatta ttatctgtta tacctggttt gggacagttt tataataaaa
     4261 gacccattaa agggacgata ttttttatct ttttcatcag ttttatttct gttttttata
     4321 gctttttaaa tattggtttt tggggattgt tcacattagg gacagtacct aagttagacg
     4381 attctcgtgt cttacttgca caaggtatta tttctatctt actcgttgct ttcgcaatca
     4441 tgctatatat cattaatatt ttagatgcat atcgtaatgc tgaacgattt aatcgcaatg
     4501 aggaaataaa ggatccgaag gcgcgtatgg tggcaacatg ggacaagacg ttcccatact
     4561 tactaatctc accaggtaca ttcttattga tatttgtagt tgtatttcca ttaatattta
     4621 tgtttggagt agcatttaca aattacaatt tatacaacgc gcctccgaga cacacattag
     4681 aatgggttgg tttagataac tttaaaacgt tattcacaat tggcgtttgg cgtaaaacat
     4741 ttttcagtgt tattacttgg acattagtat ggacgcttgt tgcaacgaca cttcaaattg
     4801 cattagggct gtttttggca attattgtaa atcaccctgt cgtcaaaggt aagaaattta
     4861 tccgtactgt gttaatccta ccttgggctg taccatcatt tgtgacaatt ttaatatttg
     4921 tagcgttatt taatgatgaa tttggtgcga taaataatga tattttgcaa cctttattag
     4981 gtgtagcacc agcatggtta agtgatccgt tttgggcaaa agtggcatta atcggcattc
     5041 aagtatggct tggattccca tttgtctttg cactgttcac tggagtactg caaagtattt
     5101 catcagattg gtacgaagca gcagatatgg atggtgcgtc tagttggcaa aagtttagaa
     5161 acatcacatt cccgcatgtc atttacgcca cagcgccatt gttaattatg caatatgcag
     5221 gtaatttcaa taattttaat cttatttatc tatttaataa aggcggtcca ccagtgtcag
     5281 ggcagaatgc tggtagtaca gatatcttga tatcttgggt gtataatctg acatttgagt
     5341 ttaacaactt caacatgggt gcagttgtgt cattaattat tggatttatt gttgctattg
     5401 tcgcatttat tcaattcaga cgtacaagta cgtttaaaga tgagggaggt ttataagatg
     5461 acaaagaaga aaaacatatt aaaagcaatc ggtatttaca gttttatagc gatgatgttt
     5521 gtcatcattt tatatccact actgtggaca tttggcattt cccttaatcc aggtacgaac
     5581 ttgtatggtg ccaaaatgat accagacaat gcaacattta aaaattatgc attcttacta
     5641 ttcgatgaca gtagtcaata cctgacttgg tataaaaata cgcttatcgt agcatctgca
     5701 aatgcactgt ttagtgtgat atttgtcacg ttaacagcat atgctttttc tagatatcgc
     5761 tttgttggtc gtaaatacgg gctgattaca tttttgattt tacaaatgtt ccctgtatta
     5821 atggcaatgg tcgcaatcta tattttgcta aatacaattg gattattaga ttctttattt
     5881 ggactaacac tggtatatat tggtggatca ataccgatga atgccttttt agtgaaaggt
     5941 tacttcgata cgattccaaa agaacttgat gaatctgcca aaattgatgg tgcagggcat
     6001 atgcgtattt tcttacaaat tatgcttcca ttagctaagc cgattttagc agttgttgct
     6061 ttgttcaatt ttatggggcc atttatggac tttatattac ctaaaatact attaagaagt
     6121 cctgaaaaat tcacattagc agttggattg ttcaacttta ttaatgataa gtatgcaaat
     6181 aatttcacag tgtttgcagc aggggcaatt atgattgcag tacctatagc aatcgtattc
     6241 ttgttcttgc aacgctattt agtatcaggt ttaacaacag gtgcgacaaa aggttagttt
     6301 gaaattagga gtggggcaga attgataaag aaccactaat gacgataaag attaaaagga
     6361 ggacgttatg atgacgatta aagttggaat cattgggtgt ggtggtattg cgaatggcaa
     6421 gcacatgcca agtttacaaa aagttgaaaa tgttgaaatg atcgcatttt gtgacgtaga
     6481 catttcgaaa gcagcgagtg cggcagaagc atacggaact gacaatgcaa aggtttatga
     6541 tgattacaaa gcattgttaa aagatgacac gattgatgtt atccatgttt gtacgccaaa
     6601 tgactcgcat tgtgaaatta ctgtagcagg gttgcatgct ggtaaacatg tgatgtgtga
     6661 aaaaccaatg gctaaaacga cagcagaagc tcaaaaaatg atagatacag ctaaatcaac
     6721 aggtaaaaaa ttaacaatag gttatcaaaa tcgtttccga gcagatagtc aatttttaca
     6781 tcaagcagcg caacgtggcg acttaggaga catttacttc ggaaaggcac atgccattcg
     6841 tcgtcgagca gtaccaacat ggggtgtctt tctagacgaa gaagctcaag gtggaggacc
     6901 attaatcgat atcggtacac acgctttaga tttaacgtta tggatgatgg ataattatga
     6961 accagaatca gtgatgggtt caacattcca taaattaaat aaacagcatc atgcggcaaa
     7021 cgcttggggt tcatggaatc cagatgaatt tacagttgaa gattctgcgt ttggatttat
     7081 taaaatgaag aatggagcga cgatcatttt agaatccgct tgggcgatta attctttaga
     7141 agtggatgag gcaaaatgtt cattatcagg aactaaagca ggtgctgata tgaaagatgg
     7201 tctacgtatt catggtgaag acatgggtac actttatacc aaacacgttg aattggaaaa
     7261 caaaggcgtc gacttttatg aaggtaatga agtggatgaa gctgaagaag aagcaaaagc
     7321 ttggattgat gcagttgtaa atgatactga accagttgtg aaaccggaac aagcaatggt
     7381 agttacaaaa attcttgaag cgatttatca gtctgcaaaa tcaggcaaag caatttactt
     7441 tgaataacat catacggtaa ggaggcacat catgacaaaa ttaaaagttg gtgtgatagg
     7501 tgttggtggt attgcacaag accgtcatat tccagcattg ctgaaactca aagacacagt
     7561 ctcattagtt gcagtacaag atattaatac agtgcagatg attgatgttg cgaagcgctt
     7621 taatatacct catgcagttg agacacctag cgagctgttt aaacttgttg atgcggtggt
     7681 catttgtaca cctaataaat tccatgctga tctttctata gaagcattga accatggtgt
     7741 ccatgtattg tgtgaaaagc caatggcgat gacgacggaa gagtgtgatc gcatgattga
     7801 agcggctaat aaaaatcaca aattattaac tgtcgcatat cattatcgtc acacagatgt
     7861 ggcaattact gctaaaaaag caattgaatc aggtgtggtt ggtaaacctt tagtagcacg
     7921 tgtacaagcg atgcgtaggc gtaaagtgcc tggctggggt gtttttacca ataaagcgtt
     7981 gcaaggtggc ggtagtttaa tcgattatgg ttgccacttg ttagacttat ctttgtggct
     8041 actaggtaaa gatatggtgc cgcatgaagt gctaggaaaa acatataatc aattgagcaa
     8101 acaaccgaat caaattaatg attggggaac atttgatcat actaaatttg atgtcgatga
     8161 tcatgttact agttatatga catttgccaa tcgagcaagc atgcagtttg aatgttcgtg
     8221 gtctgcaaat atcaaagaag ataaggttca cgttagttta tcaggagaag atggcggtat
     8281 caatttattt ccatttgaaa tatatgagcc ccgctttgga actatttttg aaagcaaagc
     8341 taatgttgag cataacgaag acattgctgg tgagagacag gcgcgtaact ttgtcaatgc
     8401 gtgtttaggg atagaagaga ttgtggtgaa accggaagaa gcacgcaatg taaatgccct
     8461 tatagaagcg atttatcgta gcgatcttga taacaagagc atacaacttt aatgattatc
     8521 atatatgata caaaattctc aatataaaaa gaaggagtgc ttttcaatga aaataggtgt
     8581 attttcagta ttattttacg ataaaaattt tgaagatatg ttagattatg tctcagaatc
     8641 tggattggat atgattgaag ttggaacagg tggtaaccca ggagataaat tttgtaagtt
     8701 agatgagttg ttagaaaatg aagacaagcg ccaagcattt atgaagtcaa tcacagacag
     8761 aggcttacaa ataagtggtt tcagttgtca taacaatcca atttctccag atccgataga
     8821 agcgaaagaa gccgatgaaa cgttacgtaa aacaatccgt ttagcaaatc tattagacgt
     8881 gccagttgtt aatacatttt ctggcattgc aggatcagat gataccgcta aaaagcctaa
     8941 ttggcctgtt acaccttggc caacagccta ctctgaaatt tatgattatc agtggaatga
     9001 aaagttgata ccatattggc aagatttagc tgagtttgca aaagagcaag atgtaaaaat
     9061 tgccatagag ttgcatgcag gatttttagt gcatacacca tatacaatgt tgaagttacg
     9121 tgaggctaca aatgaatata tcggtgctaa cttagatcct agtcatctat ggtggcaagg
     9181 tattgaccca attgctgcga ttcgcatatt aggccaagca aatgcaattc atcacttcca
     9241 tgctaaagat acgtatatta atcaagaaaa tgtaaatatg tatggtctaa ctgatatgca
     9301 accatatggt aacgttgcga caagagcatg gacattccgt acagttggtt atggacatag
     9361 tccatatgta tgggcagata tcataagtca acttattatt aatggatatg attatgtatt
     9421 aagtattgaa catgaagatc ctattatgtc agtagaagaa ggtttccaaa aagcttgtca
     9481 aactttgaaa tctgttaata tttacgacaa gccagcagac atgtggtggg cataatacga
     9541 actcgaggtt agtctgaagt ttgtctgaag taagactggt ggcagtgttg aataaatgca
     9601 tatgtcgcca agccattgcc aaaaatttca caccttaaat catgtcattg tttgtaaaga
     9661 aggtgtactt tatataagtg tttagcgatg gtcataccca ttcacagtaa caatcctcac
     9721 cattgaaaag agtatataac cttttcgata gtgaggtata tgataataaa aaaagcctgt
     9781 tgtcacaatg gtcatagaca cgacatactt taaaggcttc tgaatataat atttcagaac
     9841 gcactttaaa gatggacgtc gatgtcgact aatgtgatga caggctttca tctttttaaa
     9901 tattcattaa tttctcttct tgtttaatac gtacatataa gaaatacgca tacggtacta
     9961 ataaaatagt tgtatatgtt gcgtgtgtta ataataatac accgattaat tcaggaatga
    10021 tgtttaagaa gtaatttggg tgttttgtaa ttttatataa tccagattta ataataggat
    10081 ggttaggtaa aatgaataat tttaatgtcc aaataccacc taaagtttta ataaccataa
    10141 ataacatgat ataagcaaag attaatataa ctaagccaat accatttgca aagctaaatg
    10201 tatctttatt aataaatgcc tctacaccag ccaatacata aattaaaacg tgtgttattg
    10261 ctaaaaactt cgaattttta acgccatatt caactgcacc gtctgctttt aattgttttg
    10321 agtgattaat agatatcttt aagctgacaa gtctgataca gaaaaagata agtaatatag
    10381 atagaatcat gatgtcctcc gtcattatgt catatgtata agcgttgatt ttgacaacat
    10441 aaagtatttt atagataaaa cttgtcaaat actattaact atttattaat tttagtacat
    10501 aaatatgttt ctaagtatgt gtttatgttc agtattttgg ataatttaat aattttaagg
    10561 atattaagcg cttacaccga cgtgatatat ttggcttaac gaaaatgatt gaggtgacag
    10621 agatgaactt ttttgatatc cataagattc cgaacaaagg cattccatta tcggtacaac
    10681 gtaaattatg gcttagaaac ttcatgcaag ctttctttgt agtgttcttt gtttatatgg
    10741 ctatgtattt aattcgaaac aactttaagg cggcccaacc gtttttaaaa gaggaaattg
    10801 gattatctac attagaactt ggttatatcg gattagcatt tagtatcacg tacggtttag
    10861 ggaaaacatt acttggatat tttgtcgatg gacgtaacac aaaacgtatt atctcgttct
    10921 tacttatctt atctgcgatt acagttttaa ttatgggatt tgttttaagt tactttggtt
    10981 ctgtaatggg attattaatt gtactttggg gacttaacgg ggtgttccaa tcagttggtg
    11041 gacctgcaag ttattcaacg atttcaagat gggcgccaag aacgaaacgt ggccgatact
    11101 taggattttg gaatacatca cataatatcg gtggtgccat tgcaggtggt gttgcacttt
    11161 ggggtgctaa tgtattcttc catggaaatg ttatagggat gttcattttc ccatcggtga
    11221 ttgcattact tattggtatc gcaacattat ttatcggaaa agatgatcca gaagaattag
    11281 gatggaatcg tgctgaagaa atttgggaag agccggtcga taaagaaaat attgattctc
    11341 aaggtatgac gaaatgggag atctttaaaa aatatatcct gggaaatcct gttatatgga
    11401 ttctatgtgt ttcaaacgtc tttgtataca ttgtacgaat cggtattgat aactgggcac
    11461 cgttatatgt gtcagagcat ttacacttta gtaaaggcga tgcagttaat acgatattct
    11521 actttgaaat tggtgcatta gttgcaagtt tattatgggg ctacgtatca gacttattaa
    11581 aaggtcgtcg tgcaattgta gctattggct gtatgtttat gattacattt gttgtcttat
    11641 tctacacaaa tgctacaagt gtcatgatgg ttaacatttc attgtttgca ttaggtgcgt
    11701 taatctttgg tccgcaatta ttaattggtg tatcattgac tggttttgtt cctaaaaatg
    11761 ccatcagtgt agcaaacgga atgacaggtt cattcgcgta tctattcggt gactcaatgg
    11821 cgaaagttgg tttggcggct attgctgatc caacacgtaa cggtttaaac atctttggat
    11881 atacattaag tggatggaca gatgttttca tcgtcttcta tgttgcatta ttcctaggca
    11941 tgattctatt aggaatcgtt gctttctatg aagaaaagaa aattagaagt ttaaaaattt
    12001 aatataaatc ggattaaaag tatcgccaat ctattgcaat atagttggca atcctgcccc
    12061 gacggcatgt gcgtgaagag atgaaagata ctgcttctac ccttgcaaat atatcatctc
    12121 tatgtctcgg ggcagatcat aattccctgt tatgaagtat ccttatttgc ccgacttagg
    12181 gtgactcaat gaatttactc cttacaataa agacatatag cggtgtcaat attgtaggga
    12241 gtattgtttt atatttaaac tctctaaaaa gcggactgaa agaaaagtga aaacttctct
    12301 atcagtccgc tttttcatag aacaaaatgt aggcgccata atcattagtt atgtgctaat
    12361 ctattttgct tgcttacaat aatcacttgg cgacatttgt aaatattttt taaaatgata
    12421 gctaaacatt ttatactctg aaaagcctac tttgtctgca atttcatagt gtttgtaatg
    12481 tcgatctaac aattgcagag attgtaaaat acgatagcga tttaaataat cgacaattgt
    12541 aataccaaca tgatctttaa atgttcgcat cgcatacgat tcactaacat cgatatgttg
    12601 aattaaatct gaaacagtca ctttcgtttg ataagattgc ttaatttgat caacaatttg
    12661 atttacataa taatcatcgt attctacttt taataatggt tggaaggcat catgacaaga
    12721 tgctaagcta cggccgttct gtgattgttg ctctaataag gtacggacaa gtcttcctaa
    12781 aataacttct aattgtgcat ggtccactgg ttttaataaa taatcaagaa catgatgttg
    12841 aataccggct ttcatatatt caaagtcatc gtaactcgat aatatgatga cattacaatc
    12901 tagatgcgca atatcattga gtaaatcgac gccattttta cgtggcatac gaatatcagt
    12961 aattactaat tctggctgat gttgttgaat tagtgataat gcttcaacac catctttagc
    13021 agtgtatatt gtattgaaat gatagtctcc ccaaggaatg atttgcttta aaccttctcg
    13081 aataattcgt tcatcatcac aaataactac cttaaacatc tacattcccc cttgaaagtg
    13141 gtattttata acaaattaac gtaccttgat tacgctttga aaaaatatgg agtcgtgcat
    13201 gtgaaccata ttgaatcatt gctttattgt gtaaatgatt taatcccaaa tgcttagtat
    13261 caaatacatc attattaaga gattggcgta catattgcag gcgagatgac gacatcccaa
    13321 taccattgtc gcaaactaaa acatgtaaat tctgacgtgc caatgtcagg cgtatagtaa
    13381 tatccaatga ctcagtatct ctaccatgtt taatagcatt ttctatgagt ggttgaagca
    13441 tcattttacc aattgtctgg tgacgcgctt cttcagaact ttcgatatgg agcttaatca
    13501 tgtcatcaaa acggatgttt tgtatcgcaa catactgttc aatgtagttc aactcttcgt
    13561 ttaattccac tgtatgtgag tttgtacgta atgagtaacg taacatttgc gataattgtt
    13621 ggaccacagt ttgtgctaat ttcggagata acgtaattaa atattgtatt gtttgcatcg
    13681 tattgaatag gaaatgaggt tggaattggc gttctatttc ctttaactga atatcacgca
    13741 agcgacgttc tgtatgctcg atagaatgga tcagttgctc atttgattca aataaatcgt
    13801 aaatataatt attaatttct tctagttcac tgttgttttt taaaggtgta tatgtaccta
    13861 gatgacgatt tttggcatag taaatttttt gaataatcgt ttcgatatct tttgtttgtc
    13921 gtttagccat attatctgcg ctaatgaaac caaatattac tagtaaaaca agaactacgg
    13981 ccataacaat taacaacgtg ataccatctt caatgttttc atgtatatct ttataaataa
    14041 tgagacgatg gtcagcatgg tttaatttta cagattcatt cataaatccg aattgttgtg
    14101 gtctatactt ttcacctata gtaaaacggt catcgttggc gtataaaata ttgtcatatt
    14161 gatcaacgat aagtgcgaat tgtcggctat ctttcttaat ttcacttaaa cgtggggtgt
    14221 tagccatata aattttaagc atatatgtac tatttttgaa tttaagctga tgcgttgaaa
    14281 ataaatacat atttttagtg tttaaatgtt cataattatt ggttataaac tgatttggtc
    14341 cagataattc ataataaagt gttgcgggct gttggtgtat taattttaat aatccacgtt
    14401 ttgtagcggt aacatcatga tgatttgtta aatcgagctc ttgaaacgaa ttattatgct
    14461 gtgtaataaa tgtctgaatc tgcttttcag tgtgatgtaa agatgactga ctttcatcaa
    14521 catgttgatg aatcgtacga tgctcaatcc aaatatagat ggcatagaag cttactagtc
    14581 caataataat gactaaaaat accggaaaaa tagtagacgc aaataacgat cgtctcaatt
    14641 gatgtctata aggtttgtat gccgtcattg aatcatctcc aaaaatttat gatgtggaat
    14701 gtccggtaat ttagatttcg gtattaaagg tatgttctta agattttcga tagactgatc
    14761 gctttgttca ctaacatcct ttcgaattga cttggcatcg aactctgcaa ctaatcgttg
    14821 ttgtactgag cggcttgtta aatattgcac taacttttta cgcttaggat gagggtgtgc
    14881 atttttaact aaagcaatac catcaacatt taacattgtt ccttcaattg gataaacgat
    14941 tgatacagga taacctttgt ttttccatgt gcgtgcatct tgttcgtagc ttagacctgc
    15001 gtaatattta ccttttgcaa catcttcaat gactttagac gtctttgaca gttgcatcgc
    15061 atggttttgg aattgatgca catcacttac tcgatgatgc atgctataaa tagcgcgcat
    15121 atgttgatag cctgtcgttg ttgtatttgg atttgagtac gcaattttac ctttaagtat
    15181 aggttgtaat aaatcttgat aacctcgaat cttaatatct ccttgtaaat ctgaattcac
    15241 tactataact gttggcatta atagaaaact agtaacatat ttattgttcg agcgataatc
    15301 ctctaattgc tgtgttacag atgtatcttg atagggaaca aaatcttctg gatgatcaat
    15361 tgtttctgat aacacaccac ccataaagac gtcaccacgc tccgaaaaat cttcgttatg
    15421 caagtttgaa agcagtactt gagtagatcc atgtttgatt tcaattttga catgctcttg
    15481 tttttcaaat tcatttaaaa ttggacgaat caagtttgat tgatacggag aataaactgt
    15541 taatacattt ttatctgatt cagagtgacg cgtattagcg catgctgata aaaaaatgag
    15601 aaataatagc aagatataaa tttttgattt catgatatcc catcaattct atgtatattt
    15661 taatacaata attttagcaa taaatgacgc ataagtaatg ttaaatattt agaaatgttt
    15721 aaagatgact tgttaagacg ttgcaaatgt tgtgatagca cataattttt gtttgtcaag
    15781 atgacttacc gaggttgtaa aatcaaactg ttatatttta tttgtagctg ttatataaaa
    15841 attggcaaga tattgaacgg ttcaaaagta tatttttacg tcaataaaag tatttaatcc
    15901 agtctcttca tatataaaag taaatctttc taagtgttga tttaacgctt atcaacaatc
    15961 attttttata aacaaatata tactcctaaa ttaactttta aagcaatgaa aatagtgaac
    16021 attataactg ttgtgtaaca gaatgcaatt agcatattac tgttacacaa attagtacag
    16081 tttctatgtt ttgacataca tttgatgaaa attgtacata atttatgtga aaaaaatcac
    16141 aacaaacatg ctacaatgac tatgaaaacg ttaacatagc atttcaaatt cacaacatta
    16201 tacagatgga ggcgtttagt atgttagaaa caaataaaaa tcatgcaaca gcttggcaag
    16261 gatttaaaaa tggaagatgg aacagacacg tagatgtaag agagtttatc caattaaact
    16321 acactcttta tgaaggtaat gattcatttt tagcaggacc aacagaagca acttctaaac
    16381 tttgggaaca agtaatgcag ttatcgaaag aagaacgtga acgtggcggc atgtgggata
    16441 tggacacgaa agtagcttca acaatcacat ctcatgatgc tggttattta gacaaagatt
    16501 tagaaacaat tgtaggtgta caaactgaaa agccattcaa acgttcaatg caaccattcg
    16561 gtggtattcg tatggcgaaa gcagcttgtg aagcttacgg ttacgaatta gacgaagaaa
    16621 ctgaaaaaat ctttacagat tatcgtaaaa cacataacca aggtgtattc gatgcatatt
    16681 ctagagaaat gttgaactgc cgtaaagcag gtgtaatcac tggtttacct gatgcatacg
    16741 gacgtggacg tattatcggt gactatcgtc gtgtagcttt atatggtgta gatttcttaa
    16801 tggaagaaaa aatgcacgac ttcaacacga tgtctacaga aatgtcagaa gatgtaattc
    16861 gtttacgtga agaattatca gaacaatatc gtgcattaaa agaattaaaa gaacttggac
    16921 aaaaatatgg tttcgattta agccgtccag cagaaaactt caaagaagca gttcaatggt
    16981 tatacttagc ataccttgct gcaattaaag aacaaaacgg tgcagcaatg agtttaggtc
    17041 gtacatcaac attcttagat atctatgctg aacgtgacct taaagcaggc gttattactg
    17101 aaagcgaagt tcaagaaatt attgaccact tcatcatgaa attacgtatt gttaaatttg
    17161 ctcgtacacc tgattacaat gaattattct ctggagaccc aacttgggta actgaatcta
    17221 tcggtggtgt aggtattgac ggacgtccac ttgttacgaa aaactcattc cgtttcttac
    17281 actcattaga taacttaggt ccagctccag aaccaaactt aacagtatta tggtcagtac
    17341 gtttacctga caacttcaaa acatactgtg caaaaatgag tattaaaaca agttctatcc
    17401 aatatgaaaa tgatgacatt atgcgtgaaa gctatggcga tgactatggt atcgcatgtt
    17461 gtgtatcagc gatgacaatt ggtaaacaaa tgcaattctt cggtgcacgt gcgaacttag
    17521 ctaaaacatt actttacgct atcaatggtg gtaaagatga aaaatctggt gcacaagttg
    17581 gtccaaactt cgaaggtatt aacagcgaag tattagaata tgacgaagta ttcaagaaat
    17641 ttgatcaaat gatggattgg ctagcaggtg tttacattaa ctcattaaat gttattcact
    17701 acatgcacga taaatacagc tatgaacgta ttgaaatggc attacatgat acagaaattg
    17761 tacgtacaat ggcaacaggt atcgctggtt tatcagtagc agctgactca ttatctgcaa
    17821 ttaaatatgc acaagttaaa ccaattcgta acgaagaagg tcttgtagta gactttgaaa
    17881 tcgaaggcga cttccctaaa tacggtaaca atgacgaccg tgtagatgat atcgcagttg
    17941 atttagtaga acgctttatg actaaattac gtagtcataa aacatatcgt gattcagaac
    18001 atacaatgag tgtattaaca attacttcaa acgttgtata cggtaagaaa actggtaaca
    18061 caccagacgg acgtaaagct ggcgaaccat ttgcaccagg tgcaaaccca atgcatggcc
    18121 gtgaccaaaa aggtgcatta tcttcattaa gttctgtagc taagatccct tacgattgct
    18181 gtaaagatgg tatttcaaat acattcagta tcgtaccaaa atcattaggt aaagaaccag
    18241 aagatcaaaa ccgtaactta actagtatgt tagatggtta cgcaatgcaa tgtggtcacc
    18301 acttaaatat taacgtattt aaccgtgaaa cattaataga tgcaatggaa catccagaag
    18361 aatatccaca gttaacaatc cgtgtatctg gttacgctgt taacttcatt aaattaacac
    18421 gtgaacaaca attagatgta atttctcgta cattccatga aagtatgtaa caaaatttaa
    18481 ggtgggagca ctatgcttaa gggacactta cattctgtcg aaagtttagg tactgtcgat
    18541 ggaccgggat taagatatat attatttaca caaggatgct tacttagatg cttgtattgc
    18601 cacaatccag atacttggaa aattagtgag ccatcaagag aagtcacagt tgatgaaatg
    18661 gtgaatgaaa tattaccata caaaccatac tttgatgcat cgggtggcgg tgtaacagtc
    18721 agtggtggcg aaccattgtt acaaatgcca ttcttagaaa aattatttgc ggaattaaaa
    18781 gaaaatggtg tgcacacttg cttagacaca tcggctggat gtgctaatga tacaaaagca
    18841 tttcaaaggc attttgaaga attacaaaaa catacagact tgatattatt agatataaaa
    18901 catattgata atgacaaaca tattagattg acaggaaagc ctaatacaca catccttaac
    18961 ttcgcgcgca aactgtcaga tatgaaacaa cctgtatgga ttcgacatgt ccttgtgcct
    19021 ggttattctg atgataaaga cgatttaatt aaactagggg aattcattaa ttctcttgat
    19081 aacgtcgaaa agtttgaaat tctgccatat catcagttag gtgttcataa gtggaaaaca
    19141 ttgggcattg catatgaatt agaagatgtc gaagcgcccg atgatgaagc tgttaaagca
    19201 gcctaccgtt atgttaactt caaagggaaa attcccgttg aattataaat acaattcaga
    19261 ccgaaaagaa agcatatgca acttcaagag tgaaggggca tatgcttctt tttcaattga
    19321 gtatagagta ttagcaagac gtagtaagta tatgagacaa cttctacaat ggttgaagga
    19381 agacgttttt gtaagtagct atgctgataa agaatgtgat gtcttgttaa aggtggggtt
    19441 ccaatatcat catttagctg atgttgaatg ggttattatt tgctacttgc atatgaatat
    19501 gagtcttttc aaatttttat tgaccctgag taatgaaaaa tattaagatg aaacttaata
    19561 ttaaagcaat gcggagcgtg attatgaaga gaattagtaa agatatatgg gcagtattta
    19621 aattactgta tcaaaataaa gggcgtttta gcattaatgc cttactattg cagttaatca
    19681 tgatttttat tagtagtaca tacttaattt tactatttaa tatgatgtta aaagtagctg
    19741 ggcaaagcca acttacgatt aacaattgga cggaaatcgt tagtcatccc gccagtgtga
    19801 tacttcttat tatattcata ttaagtgttg cctttctgat ttatgtagag ttttcattgt
    19861 tagtttatat ggtttatgcc ggctttgatc gacagattat tacatttaaa tccattttta
    19921 aaaatgcctt tgtaaatgtg cgtaaactca taggtgtacc agttattttc tttgttattt
    19981 atttaatgtt aatgataccc attgccaacc taggactaag ttcagtatta acaaaaaata
    20041 tttacatacc taaattttta acggaagaac ttatgaaaac gacgaaaggt ataatcattt
    20101 acggtacctt tatgattgct gtatttatat taaactttaa attaatattt accttaccgt
    20161 taacgatttt aaaccgtcag tcgttattta aaaatatgag actaagttgg caaattacga
    20221 agcgaaataa gtttcgactt gttatagaaa tagttatatt ggaactcatc attggtgcga
    20281 ttttaacatt aattatttca ggagcaacat atcttgctat ttgtgtagat gaagaaggag
    20341 ataagttttt agtctcatca attttatttg ttgtattgaa aagcgcattg ttcttctatt
    20401 atttatttac gaaattatca ttaatcagtg tgttagtact gcacttaaaa caagagaatg
    20461 tattagacca accgggctta gaatttaaat acccaaaacc gaaacggaag tctaggttct
    20521 ttataatttc aatggtactt gcagtgacat gttttatcgg ttataacatg tacttacttt
    20581 acaataatac tatcaataca aatatctcca ttattggcca tcgtggtttc gaagataaag
    20641 gcgttgaaaa ttctattccg tcattgaaag ctgctgcaaa agcgaatgtc gaatacgttg
    20701 agttagatac aattatgacg aaagataaac aatttgttgt tagtcatgat aacaatttga
    20761 aacgtttaac aggtgttaat aaaaatattt ctgaatctaa tttcaaagat gtcgtcggtt
    20821 tgaaaatgcg tcaaaatgga catgaagcaa aacttgtatc cttagacgaa tttattgaaa
    20881 cggctaaaca atcaaatgtg aagctactag tagagttaaa gccacatggt aaagaaccag
    20941 cagattatac acaacgtgtt attgatattt tgaaaaagca tggtgttgaa catcaatatc
    21001 gtgtgatgtc attggattat gatgtgatga ctaagttgaa aaaagaagcg ccatatctca
    21061 agtgtggtta tatcattccg ttgcagtttg gtcattttaa agaaacatca ttagatttct
    21121 ttgtcatcga agatttttct tattcgccaa gacttgttaa tcaagcgcac ttggaaaata
    21181 aagaagtcta tacttggacc attaacggcg aagaagattt aacgaaatac ttacaaacca
    21241 atgttgatgg tattatcaca gatgacccag cattagctga tcagattaaa gaagaaaaga
    21301 aagacgaaac atacttcgat cgttctataa gaattttgtt tgaataatat aaacaaagac
    21361 ctctaaagtt atcaagatga taccttcaga ggtcttttta atgttgccat ctatgggata
    21421 ggcaatcgtt tcattcgttt atattcatat gacaagtatt tgtatggcaa tttggcgtca
    21481 caaacactta catgatttat tgataagttg ctaattgttt tgtgaatgca aagggttaga
    21541 aattgaatcg taaatacttt ctaatctatg tttcgcttta gtcatttgat ccaaattttt
    21601 agtgcgtata gcggattttg caatatagtg cgcagctaaa atatcgcgtt tttgaaacgc
    21661 atctaaattt aggtacgata atttatttaa gtcagtgttt gctattaatt catgtaattg
    21721 atctacaagc gcttgatgtt gatacgtatg tgatgtagtt tcagatttgc ttgctaattt
    21781 aataccagtc gtatcaagga gcgccgcttt aataccagca actaaatatg ttttgatttt
    21841 catttgtgtt gtcatgcttt gttactcctt tgatgtacat taatcaaaaa aattatacac
    21901 tattgtatat tgcaaagcta attaactata acaaaaagat agttaatgct ttgtttattc
    21961 tagttaatat atagttaatg tcttttaata ttttgtttct ttaatgtaga ttgggcaatt
    22021 acattttgga ggaattaaaa aattatgaaa aagcaaataa tttcgctagg cgcattagca
    22081 gttgcatcta gcttatttac atgggataac aaagcagatg cgatagtaac aaaggattat
    22141 agtgggaaat cacaagttaa tgctgggagt aaaaatggga aacaaattgc agatggatat
    22201 tattggggaa taattgaaaa tctagaaaac cagttttaca atatttttca tttactggat
    22261 cagcataaat atgcagaaaa agaatataaa gatgcagtag ataaattaaa aact
//
LOCUS       DBNENW010000030        22268 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.030, whole genome shotgun sequence.
ACCESSION   DBNENW010000030 DBNENW010000000
VERSION     DBNENW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.030"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1061
                     /locus_tag="AC1LW8_000910"
     CDS             <1..1061
                     /locus_tag="AC1LW8_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772764.1"
                     /translation="NAIIYKEKQPVSVTVLFYVLCFFGQLFMENVTLFHCIILILAFL
                     YEFTANRTLNYKLLFSFMIAIIGTIIMFSNPNYRKILFEGSEYQQVSNNQGIFSKFAE
                     MISTSLPYGVIFSQIIILSMIAALIIYLLLRSDRYVHLTILKRRIIMIGFITLPLYYL
                     LFYNQFLLNKNTDIGLVGFVNVIVCGYFVFSIFVGIYLSINDRKTQITLYSLLIAICV
                     SASTLVIVTPIEPGNFLIVYTIHVIILIILLKEFRKYKSINVNFIKGASLVLAVIYLS
                     AFIYVHYEHEKRVQLLKQQIKDHPRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYE
                     ELPKMTKVKYIPFGSDDS"
     gene            1355..3985
                     /gene="polA"
                     /locus_tag="AC1LW8_000911"
     CDS             1355..3985
                     /gene="polA"
                     /locus_tag="AC1LW8_000911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIP4772765.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            4001..4873
                     /gene="mutM"
                     /locus_tag="AC1LW8_000912"
     CDS             4001..4873
                     /gene="mutM"
                     /locus_tag="AC1LW8_000912"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIP4772766.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEVL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            4889..5512
                     /gene="coaE"
                     /locus_tag="AC1LW8_000913"
     CDS             4889..5512
                     /gene="coaE"
                     /locus_tag="AC1LW8_000913"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIP4772767.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            5682..6707
                     /gene="gap"
                     /locus_tag="AC1LW8_000914"
     CDS             5682..6707
                     /gene="gap"
                     /locus_tag="AC1LW8_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIP4772768.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            6918..7388
                     /gene="nrdR"
                     /locus_tag="AC1LW8_000915"
     CDS             6918..7388
                     /gene="nrdR"
                     /locus_tag="AC1LW8_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIP4772769.1"
                     /translation="MKCLKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            7389..8789
                     /locus_tag="AC1LW8_000916"
     CDS             7389..8789
                     /locus_tag="AC1LW8_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HIP4772770.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYFGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            8789..9709
                     /gene="dnaI"
                     /locus_tag="AC1LW8_000917"
     CDS             8789..9709
                     /gene="dnaI"
                     /locus_tag="AC1LW8_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HIP4772771.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNQRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            9802..9924
                     /locus_tag="AC1LW8_000918"
     CDS             9802..9924
                     /locus_tag="AC1LW8_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772772.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            10121..12058
                     /gene="thrS"
                     /locus_tag="AC1LW8_000919"
     CDS             10121..12058
                     /gene="thrS"
                     /locus_tag="AC1LW8_000919"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIP4772773.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     regulatory      12164..12339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            12483..13976
                     /locus_tag="AC1LW8_000920"
     CDS             12483..13976
                     /locus_tag="AC1LW8_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP4772774.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    14051..14176
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14205..14732
                     /gene="infC"
                     /locus_tag="AC1LW8_000921"
     CDS             14205..14732
                     /gene="infC"
                     /locus_tag="AC1LW8_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIP4772775.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            14761..14961
                     /gene="rpmI"
                     /locus_tag="AC1LW8_000922"
     CDS             14761..14961
                     /gene="rpmI"
                     /locus_tag="AC1LW8_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIP4772776.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            15008..15364
                     /gene="rplT"
                     /locus_tag="AC1LW8_000923"
     CDS             15008..15364
                     /gene="rplT"
                     /locus_tag="AC1LW8_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIP4772777.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            15506..16114
                     /locus_tag="AC1LW8_000924"
     CDS             15506..16114
                     /locus_tag="AC1LW8_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIP4772778.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNNFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            16133..17062
                     /locus_tag="AC1LW8_000925"
     CDS             16133..17062
                     /locus_tag="AC1LW8_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772779.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            17067..17177
                     /locus_tag="AC1LW8_000926"
     CDS             17067..17177
                     /locus_tag="AC1LW8_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772780.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            17225..18526
                     /gene="tig"
                     /locus_tag="AC1LW8_000927"
     CDS             17225..18526
                     /gene="tig"
                     /locus_tag="AC1LW8_000927"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIP4772781.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            18677..19939
                     /gene="clpX"
                     /locus_tag="AC1LW8_000928"
     CDS             18677..19939
                     /gene="clpX"
                     /locus_tag="AC1LW8_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIP4772782.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDNVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            20093..20683
                     /gene="yihA"
                     /locus_tag="AC1LW8_000929"
     CDS             20093..20683
                     /gene="yihA"
                     /locus_tag="AC1LW8_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIP4772783.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            20900..22246
                     /gene="hemA"
                     /locus_tag="AC1LW8_000930"
     CDS             20900..22246
                     /gene="hemA"
                     /locus_tag="AC1LW8_000930"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIP4772784.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT         8225 a         2993 c         4227 g         6823 t
ORIGIN      
        1 ttaatgcaat tatatataaa gaaaagcagc cggtaagtgt tactgtacta ttctatgttt
       61 tatgcttctt cggtcaatta tttatggaga atgtgacatt attccactgt atcattttaa
      121 ttttagcttt tctatatgaa tttactgcaa atcgaacact taattataaa ttattgtttt
      181 catttatgat tgctattata ggtacaatca ttatgttttc caatccaaat tatcgcaaaa
      241 ttttatttga aggatcagaa tatcaacaag tttcaaataa tcaaggtatc ttttcaaaat
      301 ttgcagaaat gatatcaacg tcacttccat atggtgtcat tttcagtcag attataattt
      361 taagtatgat tgcagcgctt attatttatc tactgcttag aagtgatcgg tatgtacatt
      421 taacaatatt aaagcgtcgt atcattatga taggatttat cactttaccg ctttattatt
      481 tactatttta caatcagttt ttattaaata aaaatacaga tattggattg gttggttttg
      541 tgaacgtaat tgtatgtggt tactttgtgt tttcgatatt tgtaggtatt tatttgtcta
      601 ttaatgatcg taaaacacaa atcactttat attccttact tattgcaata tgtgtttcgg
      661 cgtcgacact agtcattgtt actccaatag aaccaggtaa ttttttgatt gtttatacga
      721 tacatgtaat cattttaatt attttattaa aagaatttcg gaaatataag tcaatcaatg
      781 ttaattttat taaaggtgca tcactagttt tagctgtgat atatcttagt gcatttattt
      841 atgttcatta tgaacatgaa aaaagagttc agttattaaa acaacaaatt aaagatcatc
      901 cacgacaaga ggtttataca gtggaatcat tgccgtttga acattatatg catcatccgt
      961 caccaatcaa taaaaattac caagagtggt ttaataatta tgaagaatta ccgaaaatga
     1021 ccaaagttaa atatattcca tttggttcag acgattcata acttattctt acctataaaa
     1081 atttaaaata gcagcataaa aatattaaaa agtagacata atcatgaaat gataatgtgc
     1141 atctcaaaag ttggactttt aacctgactt tatggagtgc tcatcaagaa caatcatgat
     1201 tatgtctttt ttatttttta gctaagtcta tataggcact tgttttattt gaaattaggt
     1261 cgtgtataaa tactctatga gatagtttct ttaattacga catcacagtc gaatatgcta
     1321 aaatatatat aaattagttc tggaggttaa aattgtgaat aaattagtat taatcgatgg
     1381 taatagctta agttttagag ctttttatgc attacctttg ttatcaaaca aagcaggcat
     1441 tcataccaat gcagtatatg gttttgcaat gttactagag aagattttaa aagaagaaaa
     1501 gccaaatcat tttttagtag cgtttgatgc aggtaaaacg actttcagac atgaaaaata
     1561 tagtgaatat aaaggtggac gccagaaaac gccgcctgaa ctaagtgagc aatttcctta
     1621 tattcgccaa ttattagatg cttatcatat taaacgttat gaattagata actatgaggc
     1681 agatgatatt atcggaactt taagtaaaga agcagataaa gcgggatttc agacaattat
     1741 tattacggga gaccgagatt taacacaact tgcaacggat aatgtaacca tttattacac
     1801 taaaaaaggt gttactgatg ttgatcatta tacacctgac tttattgcgg aaaaatacaa
     1861 cgggttaaca cctaatcaaa ttattgatat gaaaggatta atgggagata cttctgataa
     1921 tataccaggt gttgcaggtg ttggagagaa aacagcaata aaattgctaa accaatttga
     1981 cacagtagaa ggtgtctatg aacatttaga tgaaatttca ggtaaaaagt taaaagaaaa
     2041 acttcaaaat agcaaagaag atgcattaat gagtaaagaa ttagcaacga ttaatgtaga
     2101 tagtccgatt gaagtaaaac ttgaagatac gttaatgact catcaagatg aacaacaaga
     2161 aaaaatcgaa ttgtttaaga agttagaatt caaacaattg ttggctgaca ttgatcaatc
     2221 agcaagcgtt gaagatgcaa tagaaaagac atttgaaatt gaaacgtctt ttgataatat
     2281 tgattttact tcattgaaag aagcggccat ccatttcgaa ttagacggtg gtaactattt
     2341 gcgaaataat attttaaaat tctctttatt tacaggtgag aaacatattg taattaatgc
     2401 ggatgacata aataattatg tcgaactagt ttcatggtta gaaaatccga attcgaaaaa
     2461 agtcgtatat gatgctaaaa aaacatatgt agcatcacat agattgggga ttgatattca
     2521 aaatatttct ttcgatatta tgttggccag ttatataatt gatccatctc gtacgattag
     2581 tgatgttcaa tcagttgttt cattgtacgg tcaaagtttt gtgaaagacg atgtgagtat
     2641 atatggaaaa ggtaagaaat ttaaggtacc tgaagatgat gttttaaatc catatgttgc
     2701 ttctattact gatgcaattt actttgcgaa accaaatatg gataaacaat tagaagaata
     2761 caatcaggta gaactcttgg ctgatttaga gctaccgcta gctaagattt taagtgaaat
     2821 ggaagaaatt ggtatattta cagatgttca tgatttagaa gaaatggaaa aagaaattca
     2881 agaaaaatta gacgtcttga ttcgaaatat ccatgatgca gctggtgaag attttaatat
     2941 aaattctcct aagcaattag gtgttgtatt gtttgagaca ttacaattac ctgttattaa
     3001 aaagacgaaa acaggatatt ctactgctgt agatgtctta gagcaattgc aaggtgaaca
     3061 tcctatcatt gattatattt tagaatatcg tcaattatct aagttacagt ctacttatgt
     3121 tgaaggattg caaaaggtaa ttagtgatga tcaacgtatc catacacgtt ttaatcaaac
     3181 tttagcgcaa actggacgtt tatcaagtgt agatcctaat ttacaaaata ttccggttag
     3241 acttgaagaa gggcgtaaaa taagaaaagc ctttaaacca acttcaaaag atagcgttat
     3301 attatcagca gattattctc aaattgaatt gcgtgtatta gcacacatta cacaagatga
     3361 gagtatgaaa gaagcattta tcaacggcga tgatattcat acagcaactg ctatgaaagt
     3421 atttggtgta gaagctgatc aagtcgatag tttaatgcgt cgtcaagcaa aagcagttaa
     3481 ctttggaatt gtttatggga taagtgatta tggtttaagt caaagtttag gtattactcg
     3541 taaaaaagca aaagcattca ttgatgatta tttagctagt ttcccaggtg taaaacaata
     3601 tatgtctgat attgtaaaag atgccaaagc tttaggttac gtggaaacat tgctacatcg
     3661 tcgacgctat attcctgata ttacgagtcg taactttaat ttacgcggct ttgctgaacg
     3721 tactgctatg aatacgccaa tacagggcag tgctgcagat atcattaaac tggcaatggt
     3781 taaatttgct caaaaaatga aagagacaac atatcaagct aaactattat tacaagtaca
     3841 cgatgaatta atttttgaag tacctaagtc agaagtagat tcatttagtg aatttgtaga
     3901 agagataatg gaaaatgcat tgcaattaga tgttccatta aaagtagact caagttatgg
     3961 tgcaacttgg tatgatgcaa aataaagtga ggttaatggt atgcccgaat taccagaagt
     4021 agaacatgta aaaagaggaa ttgaacccta cgttataaat caaaaaattg aacacgttat
     4081 attttcggat aaagtaatcg aaggtaaagc tcaaggcaag gaaacaatta taaaaggaat
     4141 tgaattagat acttttaaaa ctttatcgga aggctatacc attacaaatg tagaacgaag
     4201 aagtaaatat atcgtttttc agctagataa taaacgagaa caacgtacat taattagtca
     4261 tttaggtatg gcgggcggtt ttttcattgt agatgaactt gaagatatta tgatacctaa
     4321 ttatcgtaag cattggcatg ttatttttga gttgtcaaat gacaaaaagt taatttattc
     4381 agatatacgt cgttttggag aaattagaaa tgttgcgtct gtagcatctt atccttcatt
     4441 tttagaaata gcgcctgaac ctttttcaaa tgaagtattg acgtattatt taaatcgaat
     4501 tcatcaacag tctaataaga ataaaccaat caaacaagtg attttggatc ataaagtaat
     4561 cgcaggttgt ggaaatattt acgcatgtga agcgttgttt cgtgcaggtg ttttaccaga
     4621 taaaaaagta aaagacttaa cacaccaaca acaagaaatg gttttttatt atgttagaga
     4681 agtactagaa gaaggtatta aatatggcgg tacaagtatt tcagattatc gtcatgctga
     4741 tggaaaaact ggtgagatgc aattgcatct taatgtatat aagcagccgg tatgcaaagt
     4801 gtgtggaagt caaattgaaa caaaaatcat cgcgacaaga aatagtcatt attgtccagt
     4861 atgtcaaaaa taaagaaaga gtgaaattat gccgaaagtt attggtctaa caggtggaat
     4921 cgcctcagga aaatcaacag tatcagaact cttatccgta ttcggtttta aagtagtaga
     4981 tgctgataaa gcagccaggg aagctgttaa aaaggggagt aaaggtttag ctcaagtacg
     5041 agaagtcttt ggtgatgaag caattgatga aaatggtgag atgaatcgtc gttatatggg
     5101 tgatctagtg tttaatcatc cagaaaaacg cttagaatta aatgctatca tacatcctat
     5161 cgtgcgagat attatggaag aagaaaagca agaatattta aaacaaggat ataatgtaat
     5221 catggatatt ccattattat ttgaaaatga attggaaaat acagtagacg aagtgtgggt
     5281 tgtatacact tctgaaagta tacaaatgga tcgtttaatg caacgtaata atttgtcatt
     5341 agaagatgcg aaagcacgtg tctatagcca aatttctatt gataaaaaaa gccgaatggc
     5401 cgatcatgtt atcgataatt taggggataa acttgaatta aaacaaaacc ttgagagatt
     5461 gttagaagaa gaaggttata ttgaaaagcc gaattacgga gaagaagatt aatattacac
     5521 tataaataag tcattacttt acgtacgcgt tgatgtatgt aagtaatgac tattttttat
     5581 aaaaaagata aataaatcaa cggaaaacgc tttcaaattt catataatat gctatactaa
     5641 ttccataaag tataacacat aaagatcaag gggtgctttt aatgtcaacg aatattgcaa
     5701 ttaatggtat gggtagaatt ggaagaatgg tattacgtat tgcattacaa aataaaaatt
     5761 taaatgtagt agcgataaat gctagttatc cacccgaaac aattgcacat ttaatcaatt
     5821 acgatacgac acatggaaaa tataatctaa aagttgaacc gattgaaaat ggattgcaag
     5881 ttggagatca taaaattaaa ttggttgctg atcgcaatcc tgaaaacttg ccatggaaag
     5941 aattagatat cgatattgct atagatgcaa ctggtaaatt taatcatggt gataaagcca
     6001 tcgcacatat taaagcaggt gccaaaaaag ttttgttaac tggtccttca aaaggtggac
     6061 atgttcaaat ggtagttaaa ggcgtaaatg ataaccaatt agatatagaa gcatttgaca
     6121 tttttagtaa tgcttcatgt actactaatt gcattggtcc agttgcaaaa gttttaaata
     6181 atcagtttgg aatagttaat ggtttaatga ctactgttca cgctattaca aatgaccaaa
     6241 aaaatattga taatccacat aaagatttaa gacgtgcacg ttcatgtaat gaaagcatta
     6301 ttcctacttc tactggtgcg gcgaaagctt taaaagaagt attaccagaa ttagaaggta
     6361 aattacacgg catggcatta cgtgtaccaa caaagaatgt atcgctcgtt gatttagttg
     6421 ttgatttaga aaaagaagta actgcagaag aagtaaacca agcttttgaa aatgcaggtt
     6481 tagaaggtat catagaagtc gaacatcaac cactagtgtc tgttgatttt aatactaatc
     6541 caaattcagc tattattgat gcaaaatcaa caatggtcat gtcagggaat aaagtaaaag
     6601 ttattgcttg gtatgataat gaatggggtt attcaaatag agttgtagat gttgctgagc
     6661 aaattggagc acttttgaca tcaaaagaaa ctgtaagtgc aagttaatac aattaattta
     6721 tagcctaact ctgacttcgg agttaggttt tttatttttt aatacaataa tattgtcatg
     6781 caaattactt aataagtaac gatataatgt tttttattgt ttcattattt ggttaaatga
     6841 attaaattga gggattcttt tttcatctct agatttatag tataattagg cataatcaaa
     6901 ataaagaggg tgatgtaatg aaatgcctga aatgtaattc tacacaatct aaagttgtag
     6961 attcaaggca tgccgatgaa ttaaatgcca ttcgaagacg aagagaatgt gaaaattgtg
     7021 gaacacgttt cactacattt gaacatatcg aagttagtca gcttatagtt gtgaaaaaag
     7081 atggcacaag agagcagttt tcaagagaaa agatacttaa tggacttgtg cgttcttgtg
     7141 agaaacgacc agttagatat caacaacttg aagacataac aaacaaggtt gaatggcaat
     7201 tacgagatga aggtcatacg gaagtgtctt cacgagatat aggtgaacac gttatgaact
     7261 tgttaatgca tgttgatcaa gtttcatatg ttagatttgc atcagtctat aaagaattta
     7321 aagatgttga tcaattgtta gcatcgatgc aagggatttt aagcgaaaac aagcggagtg
     7381 atgcttaaat gggacgacaa gccttcgaat tcggcttaag accaaaggat caattcaaag
     7441 ttatgcaaca ctttgattta aatacgaatc atttagaggt attgaatcgg ctgtatacac
     7501 cgctaattgg aacacaagca gtgggtttat accattttat gactcaattc gttaaagaaa
     7561 gtcataatga aacattgatt ctttctcatt atatttttat gaatgaactt aaaattaatt
     7621 tactagagtt tagacaacaa atggacttac ttgaagcgat tggattgtta aaggcgtttg
     7681 tcaaacatga tgaacaagaa acacaatttg tttaccaatt aattcaaccg ccttcagcac
     7741 atttattttt taatgatcca atgttgtcaa tctttttata tagtgaagtc gagcatcgtc
     7801 gatttcatga gttgaagaaa tactttgagt atcaacaaat tgatttgagt gaatttaaac
     7861 aggtaacaag acaatttact gatgttttta aagtgccgtc tacaaaaata gatatcgata
     7921 caagtgatat tccgattaat gaaccgtacc aaggtatcga tttatcaaat gaatcttttg
     7981 actttgaaat gttgagacaa atgttaggta aacattttat tagtcaagac attgttacca
     8041 aagatgcaaa gcgtttaata acacaactcg cgactttata tggtttaaca gcagatggca
     8101 tgaagcatgt tattttaaat tcaattacaa gtggacaaca gttatccttt gaagaaatgc
     8161 gtaaacaagc acgctcgtat tacttaatgg aacacgaaaa tcaaatgcct aaattacagg
     8221 tgaaatctcc tgctacatca tcttcgacgg gcaaatcttc agaagtgaat ccaaagccac
     8281 aaagtgatga atggtttgag ttacttgaac aaacgagtcc aattgatatg ttagcttctt
     8341 ggtctgaatc agaaccaact atatcgcaaa agacaatggt tgaagaatta attgaacgtg
     8401 aaaaaatgtc ttttggcgtc attaatattt tgttgcaatt tgtcatgtta aaagaagata
     8461 tgaaacttcc taaagcgtat attttagaaa tagcatcaaa ttggaagaaa aaaggtatta
     8521 agacagctaa agaagcatat aattacgcta aaaaagttaa tcaacctaaa aatgagggtt
     8581 ctagcggaaa ttaccaaaag cgtggttcat actttgggca gcgtaatcgt atttcaaaag
     8641 aaaaaacacc taaatggtta gaaaatagag ataaacctag tgaagaagat tcggctaaag
     8701 ataatagcgt agatgatcaa caattagagc aagatcgaca agcatttcta gataaattat
     8761 ctaaaaaatg ggaggaggac agtcaataat gaagcaattt aaaagtataa ttaacacgtc
     8821 gcaggacttt gaaaaaagaa tagaaaagat aaaaaaagaa gtaatcaatg acccagatgt
     8881 taagcaattt ttggaagcgc atcgagctga attaacgaat gctatgattg atgaagactt
     8941 aaatgtgtta caagagtata aagatcaaca aaaacattat gacggtcata aatttgctga
     9001 ttgtccaaat ttcgtaaagg ggcatgtgcc tgagttatat gttgataata accgaattaa
     9061 aatacgctat ttacaatgcc catgtaaaat caagtacgac gaagaacgct ttgaagctga
     9121 gctaattaca tctcatcata tgcaacgaga tactttaaat gccaaattga aagatattta
     9181 tatgaatcaa cgagaccgtc ttgatgtagc tatggcagca gatgatattt gtacagcaat
     9241 aactaatggg gaacaagtga aaggccttta cctttatggt ccatttggga caggtaaatc
     9301 ttttattcta ggtgcaattg cgaatcagct caaatctaag aaggtacgtt cgacaattat
     9361 ttatttaccg gaatttatta gaacattaaa aggtggcttt aaagatggtt cttttgaaaa
     9421 gaaattacat cgcgtaagag aagcaaacat tttaatgctt gatgatattg gggctgaaga
     9481 agtgactcca tgggtgagag atgaggtaat tggacctttg ctacattatc gaatggttca
     9541 tgaattacca acattcttta gttctaattt tgactatagt gaattggaac atcatttagc
     9601 gatgactcgt gatggtgaag agaagactaa agcagcacgt attattgaac gtgtcaaatc
     9661 tttgtcaaca ccatattttt tatcaggaga aaatttcaga aacaattgaa ttttaaaatg
     9721 attggtgtat aatgaataca aatctaaatc gtttaaatga ttgaagacaa gatgatctaa
     9781 tcaatattac acagaaagcc attgtttgat gagaatatgg ttaataaatt agatgattac
     9841 tacttcattt atggtatttg taatgaatac ccggatcaag accgttatct taagcagaga
     9901 tatttaatac ataaattgta ttaaataact agggtggtac cacgacgatc tcgtcccttg
     9961 tataggggcg ggattttttg ttttttcaga cataaatgtt tgttggtgtc ataaattccc
    10021 tgtttattgt taataggttt aatgttaaaa cgatgattgt tgttcaattt tttaacgagg
    10081 tcagataaaa gtatttataa agcaaatagg agggtttaac atggaacaaa ttaatattca
    10141 atttccagat ggtaataaaa aggcgtttga taaaggtact actactgaag atatagcaca
    10201 atcaattagt cctggattac gtaaaaaagc tgttgccggc aaatttaacg ggcaacttgt
    10261 agatttaaca aaaccgcttg aaactgatgg atcaattgaa attgtgacac caggtagtga
    10321 agaagcgtta gaggtattac gtcattctac tgcacattta atggcacacg cgattaaaag
    10381 gttatatggt aatgttaaat ttggtgtagg tcctgtaata gaaggtggat tctactatga
    10441 cttcgacatt gaccaaaaca tctcatctga tgactttgaa caaattgaaa agacaatgaa
    10501 acaaatcgtt aacgaaaata tgaaaatcga acgaaaagtg gtttcacgag atgaagcgaa
    10561 agagttattc agcaatgatg aatacaaatt agaattaatc gacgcgattc ctgaagatga
    10621 aaatgtaaca ttatatagtc aaggtgattt tactgattta tgtcgtggag ttcacgttcc
    10681 atcaacagct aaaattaaag agtttaaact attatctaca gcaggtgcat actggcgtgg
    10741 agatagtaac aacaaaatgt tacaacgtat atacggtact gctttctttg ataaaaaaga
    10801 attaaaagca catttacaaa tgttagaaga gcgtaaagaa cgtgatcatc gtaaaattgg
    10861 taaagagtta gaattattca caaatagcca attagttggt gctggtttgc cattatggtt
    10921 acctaacggt gcaacaatta gacgtgaaat tgaacgttac attgttgata aagaggtgag
    10981 catggggtat gaccacgttt atacaccagt acttgctaat gttgatttat acaaaacatc
    11041 tggtcactgg gatcactatc aagaagatat gttcccacca atgcagttag atgaaactga
    11101 atctatggta ttacgtccaa tgaactgtcc acatcatatg atgatttatg cgaataaacc
    11161 acattcatat cgtgaattac ctatccgtat cgctgagcta ggaacgatgc atagatatga
    11221 agcaagtggt gctgtatcag gattacaacg tgttcgtggt atgactttaa atgattcaca
    11281 tatctttgtt cgacctgatc aaattaaaga agaattcaaa cgcgttgtaa acatgattat
    11341 tgatgtgtat aaagactttg gtttcgagga ttatagcttt agattaagtt atagagaccc
    11401 tgaagataaa gaaaagtact ttgatgatga tgatatgtgg aataaagctg aaaatatgct
    11461 taaagaggca gcggatgagc ttggcttatc gtacgaagaa gcgattggtg aagcggcatt
    11521 ctatggtccg aaactagatg ttcaagttaa aacagcgatg ggtaaagaag agacattatc
    11581 aacagcacaa cttgatttct tattaccaga acgttttgat ttaacttata ttggtcaaga
    11641 tggtgaacat catcgtccag ttgttattca tcgtggtgtt gtatcaacaa tggaacgatt
    11701 tgtagcattt ttaactgaag aaactaaagg tgcattccca acttggttag cgccaaaaca
    11761 agttcaaatc attccagtta acgttgattt acattatgat tatgcgcgcc aattacaaga
    11821 tgaattgaaa tctcaaggcg ttcgtgtaag tattgatgac cgtaatgaaa aaatgggtta
    11881 taaaattaga gaagctcaaa tgcaaaaaat accttatcaa atcgtagttg gggataagga
    11941 agttgaaaat aatcaagtga atgtgcgtca atatggatcg caagaccaag aaacagttga
    12001 aaaagatgaa tttatctgga atctagttga tgaaattcgt ttaaaaaaac atagatagac
    12061 agttgtcgca ataaaatgct ttgaaatttt tattgcgtat caagtttcac agggttgatt
    12121 atgcgtgatg aatcctgtat attacaagtt agttaaaata ttaaattgag ttagaggttg
    12181 catgtttaat tagtaacttg tcagaagtat ttatggtaca taagttgaac aagtgaaagg
    12241 taaagatgcc gaaatagata taaaccataa attatatcta ttgggacagt tttcgaatag
    12301 gaactgtact gtcacagaat gtgatgtgct accttatata gataattgcc aaagtggttg
    12361 catatcttaa aggtatgtag ccactttttt acttttaata tcactatgtt ctgtaaaaag
    12421 ggtatgaaag tgaataaagg ttatttattt cttggcctct aaaacatgga aagggagctt
    12481 atatgtcaaa agttcaaaat gaaagtaaca atgttgtcaa aaggggactt aaagatcgtc
    12541 atatttctat gattgcgatt gggggttgta ttggtacagg tttatttgta acttctggtg
    12601 gagcaattca tgatgcaggt gctttgggtg cattaatagg atacgcaatt atcggaataa
    12661 tggtattttt cttaatgacg tcacttggcg aaatggctac gtatttgcca gtatcaggtt
    12721 catttagtac atatgctaca agatttgttg atccatcttt agggtttgcg cttggttgga
    12781 actattggtt taactgggta gtaactgtag cagcagatat tacgattgca gcacaagtca
    12841 ttcaatattg gacaccattg caaggcatac ccgcttgggc atggagtgcg ttgttcttag
    12901 ttataatttt tagtctgaat tcgttatcag ttcgcgtcta tggtgaaagt gaatactggt
    12961 tggcattgat aaaagtggtt acagttattg ttttcattgc aattggttta ttaacgattg
    13021 tcggaatcat gggtggtcat gttgtaggat tcgaaatatt taataaaggt gaaggtccta
    13081 ttcttggtgg caacttagga ggaagtttat tatcaattct aggtgtattc ttaatcgctg
    13141 gtttctcatt ccaaggtact gagttaattg gtattacggc tggtgaatca gaaaatcctg
    13201 aacgtgctgt gccgaaagca attaaacaag tattctggag aattttatta ttttacattt
    13261 tagccatttt tgttatcggt atgttaattc cttatgatag tagtgcatta atgggtggta
    13321 gtgataatgt agcaacgtct ccattcacat tagtgtttaa aaatgctgga tttgcgtttg
    13381 cagcatcatt tatgaatgca gtcattttaa cgtctgtgtt atcagcaggt aactcaggta
    13441 tgtatgcttc aactcgaatg ctatattcaa tgagtaaaga taaattagcg tttgaagcat
    13501 ttggtaaaac aaataaaaac ggtgtgccat atatgtcgtt actcgttaca gcaatcattg
    13561 tagtgataat tttcgtatta caaagcctta caaaaggtgc atacgaatat atcgttgcag
    13621 caagtggttt aactggtttt attgcttggg taggtatagc agtaagtcat tatcgattta
    13681 gaagagcatt tgataaacaa aattatgaca aatctaaatt aaaatatact gctaaattat
    13741 tcccattcgg accattattt gctggctttt tatgtattgt agtaattatt ggtcaagatg
    13801 ttgactttat taaaacagga gattttaatt ttagtcgctt cttaattacg tatatgggaa
    13861 tcccagtatt tttagcattc tttatttatc ataagcttcg atataaaacg aaaaaaattc
    13921 cattagaaca agtagattta cgtcaagacg tgtcaatgga tgaaataaaa ggttaaaata
    13981 aaaataatta cagttatagt gttgacttaa atgtgaattg ttgatatgat taacaacgtt
    14041 gaatacgaaa cgaaccaagt agaagcaccc gcttctcacc tgtagcgacg cataaagcag
    14101 ttggcaggtt aatcacatta cacataactg tgtataagga agagcgggta ttttatacct
    14161 gctcttcctt tttgcttggt gcaaatttcg taaaaatttt ggaggtgtca accatagcaa
    14221 aagatcaaac tcaaatcaat gacaaaattc gtgcaaaaga attacgttta atcggtcaag
    14281 atggtgaaca aattggtgtt aaatcaaagc gtgaagcttt agaaatggct gaacgtgtag
    14341 atttagactt agtggtcgtt gcaccgaatg cgaaaccacc agttgcaaga attatggatt
    14401 acggtaaatt caaattcgaa caacagaaaa aagaaaaaga aatgaaaaag aaacaaaaaa
    14461 ttatcaatgt taaagaaatt cgtttaagtc caacaattga ggaacatgat ttccaaacta
    14521 agttgaaaaa cggacgtaaa ttcttaacta aaggcgataa atgtaaagta tctattcgtt
    14581 tcagagggcg tgccattacg cataaggaaa ttggtcaacg tgtgctagaa aaatatgcag
    14641 atgaatgcaa agatatagca acagttgaac aaaaacctaa aatggacggg cgtcaaatgt
    14701 ttatcatgtt agcgccaaca gctgaaaaat aattaaagct attacaggag gacttatatt
    14761 atgccaaaaa tgaaaactca ccgcggagca gctaaacgtg ttaaaagaac tgcttcaggt
    14821 caattaaaac gttcaagagc tttcacatct cacttattcg caaacaagag cactaaacaa
    14881 aaacgtcaat tacgtaaagc tagattagtg tctaagagcg atatgaaacg tgtaaaacaa
    14941 ttattagcat acaaaaaata agaacaaata cagaaatcgg taggaattac ctaaggagga
    15001 attttttatg ccacgagtta aaggtggaac agtaacaaga gcgcgtcgta aaaaaacgat
    15061 taaattagct aaaggttact tcggttcaaa acatacatta tacaaagtag ctaagcaaca
    15121 agtaatgaaa tcaggtcaat atgctttccg tgaccgtcgt caacgtaaac gtgacttccg
    15181 taaattatgg attacacgta tcaacgcagc agctcgtcaa catgaaatga gctactcacg
    15241 tttaatgaac ggtttgaaaa aagctggtat cgacattaac cgtaaaatgt tatcagaaat
    15301 cgcaatttct gacgaaaaag catttgctca attagtaact aaagctaaag atgctttaaa
    15361 ataataacac ttataaaaaa gagatggcat tgtcatctct tttttatttt ttaaaaagca
    15421 aaatgccaca tttaatcgct aaaatatgac atatttgcta aaatgcattt aagagaacaa
    15481 acattaaagt gaggagtttt atacgatgtc taaatttgat gaacaaatca ttgtagtacc
    15541 tagagaaatt atttttaata atgaaaagaa taatttcaat gggtttttaa ataagaataa
    15601 accagaaggt caaaatattt ttgatgcgct aagtcaatat gaagttaagc gacgcggcga
    15661 tatggaagag gatccatcat ataaacaact catttcttat tgtttacttg aaaatgagca
    15721 tggcgagata ttagtgtatg aacgattatc tggcggtgga gaagctcgat tgcatggaca
    15781 atcttcaata ggtgtaggcg gtcatatgaa tgatgttcca ggagcagaat ctattaacga
    15841 agtattgaga gttaatgcac agagagaatt agaagaagaa gtaggtttaa gtgagcaaga
    15901 ttcacaaaat atggaatata tcggttttat taatgacgat aataatgaag tgggcaaggt
    15961 acatattggt gttgtattta aaatcactgt aagtacgaat gatgtagaag ctaaagaaac
    16021 agatacttta cgaataaaat gggttgaaaa aggcaacata gagtcatatg atgatttcga
    16081 aacgtggagc gcattaatcc ttcaagattt ataatcaaac gaggtgacat atatgtcaga
    16141 tattattcag tttcctaact cttcgaaaaa gttatataaa gatataaaac gtgctgaaca
    16201 agaccaaaac tatgacctga tgtatgaata tattgtgcaa tatgaacgtc aatttgaact
    16261 cactgaagaa atagcaatga tgaaatgccg tatgttatat gagacggggt catttcttga
    16321 attaagagaa gaaacaattg tcttattgaa aactggcata caacaatatg atgcattgat
    16381 gatttattac gtgaaaagtt tgattggttt gggacaatat tttgaagcgg tagaagtaat
    16441 tcatcaaatt attgatgaag tcaaagatca caaaacaaga atggcattac atcctcttaa
    16501 agaatttgct aagtcaaaat taattgaaga tgaaaaacga ttgactcagt cattaactga
    16561 ttttgatacg ttatcaatga gggaacagac gcacttgatt ttgaagttaa tagacaatgg
    16621 tcattttcaa tttcaagaaa cggtattata tatattaaaa tctaatacgt acagttataa
    16681 cctcattagt ttaatgattg agtatttaag gtttgcaaat tgtacacaag aactgacaat
    16741 tgaaaagtat ggtatggatg taacttttgt accagctaat ttaaatgggc tagaacatac
    16801 aacactgaaa gaaaaagtta tacctaacgt tatggaaaca ttaaatgatg gtgcattaca
    16861 tatcgctgaa gaagcacacc atattatgaa taatcactct atcatgattt atccaattga
    16921 tattgaaact ttgtttgaaa cgaataaatg gattaatgca tacgaatgtt atttcaaaaa
    16981 tatgttagga cttaaatgtg aattacaaag tatcgatgca tttaatttta ttcagcaatt
    17041 agatttgaat aataatagtt aaattgttgc aaaataatac tcagcatttt aatatagttt
    17101 tgacgaaaaa agttgattta caaatatata aacgttgtga tttcaatgtt tgtataggga
    17161 atagacctat gatataatag ggaagttgaa aaaataaatt agttaatcat ggaggtatat
    17221 atacatgaca gcaacttggg aaaaaaagga aggtaacgaa ggtttattaa ctgttactgt
    17281 tcctgcagaa aaagtaaaca aagcgttaga tcaagcattc aaaaaagtgg ttaaacaaat
    17341 taacgtacct ggattccgta aaggtaaagt gccacgtcca atttttgaac aacgctttgg
    17401 tgtagaagca ttatatcaag atgctatcga cattttatta ccagatgctt atggtgaagc
    17461 aattgacgaa actgatatta aaccagttgc acaaccagaa gtaagtgtta ctcaaattga
    17521 aaaaggtaaa gatttcattt ttgaagcaac agttacagtt gagccagaag ttaaattagg
    17581 agactataaa ggtcttgaaa ttgaaaaaca agaaactgaa ttatctgatg atgagttaca
    17641 agaagcgatt gaccacagct taggacattt agctgaaatg gtagttaaag aagatggtgt
    17701 tgttgaaaat ggcgacacag ttaacattga ctttagtggt tcagttgacg gagaagaatt
    17761 cgaaggtgga caagctgaag gttacgattt agaaatcggt tcaggttcat tcatacctgg
    17821 tttcgaagag caattagaag gtatgaaagt tgacgaagaa aaagatgttg tcgtaacatt
    17881 cccagaagaa taccatgctg aagaattagc tggtaaagaa gcaactttca aaacaaaagt
    17941 taacgaaatt aaatttaaag aagttccaga attaacagat gaaattgcta atgaattaga
    18001 tgcagaagca aatacagtag acgagtacaa agaaaactta cgtaaacgtt tagctgaaca
    18061 aaaagctaca gatgctgaaa atgttgaaaa agaagaagcg attacaaaag ctactgataa
    18121 tacaacaatc gatattcctg aagcaatggt taatactgaa ttagatcgta tggtgtctga
    18181 atttgcacaa agaattcaac aacaaggttt agatttacaa acgtacttcc aaatctcagg
    18241 tcaagatgaa actcaattaa gagagcaaat gaaagacgat gcagaacaac gtgttaaaac
    18301 taacttaact ttaactgcga tcgctgaagc tgaaaaaatc gaagctactg atgaagatat
    18361 cgataaagaa ttagaaaaaa tgagtaaaca atttaatatc tcagttgaag atatcaaaaa
    18421 tactttaggt aatactgata tcattaaaaa tgatgttcgt atccaaaaag ttatcgattt
    18481 attaagagat aacgcaaagt tcgttgaagg aactaaagaa gattaatcct cattaaatat
    18541 taaattacaa aaatgagtag cagatgcata gcttatgtat ctgctactat tctttaagca
    18601 aaaagtttgt atgttaatat gttgcattgt aatatccaat ctagtatagt ctttaacgaa
    18661 taggggtgta aaaagaatgt ttaaattcaa tgaagatgaa gaaaatttga aatgctcttt
    18721 ctgcggaaaa gaccaagatc aagtaaaaaa acttgtagca ggaagtggtg tatatatttg
    18781 taatgagtgt attgaattat gctcagaaat cgtcgaagaa gaattagctc aaaacacttc
    18841 tgaagcgatg acagaattac ctactcctaa agaaattatg gatcatttaa acgaatatgt
    18901 tattggtcaa gaaaaagcta aaaaatcttt agctgtagct gtttataacc actataagcg
    18961 tattcaacaa ttaggaccaa aagaagatga tgttgaatta caaaaaagta acattgcatt
    19021 aattgggcca acaggtagtg gtaaaacatt attagctcaa acattagcca agacgttgaa
    19081 tgtaccattt gcaattgcag atgcgacaag tttaactgaa gctggttatg taggcgatga
    19141 tgttgaaaat atcttgttga gattaattca agcagctgac tttgacattg ataaagccga
    19201 aaaaggtatt atttatgtag atgaaattga taaaattgca cgtaaatctg aaaacacatc
    19261 tataacacgt gacgtttcag gtgaaggtgt tcaacaagca ttgcttaaaa tcttagaagg
    19321 tacgactgca agtgttccgc cacaaggtgg acgcaaacat ccaaaccaag aaatgattca
    19381 aattgataca acaaatatct tatttattct tggtggtgcc tttgatggta ttgaagaagt
    19441 gattaagcgc cgtcttggtg aaaaagttat tggtttctca agcaatgaag ctgataaata
    19501 tgacgaacaa gcattattag cacaaattcg cccagaagat ttgcaagcct atggtttgat
    19561 tcctgaattt atcggacgtg tgccaattgt agctaattta gaaacattag atgtaactgc
    19621 gttgaaaaac atcttaacgc aacctaaaaa tgcacttgtg aaacaatata ctaaaatgct
    19681 ggaattagat aatgtggatt tagagttcac tgaagaagct ttatcagcaa ttagtgaaaa
    19741 agcaattgaa agaaaaacag gtgcgcgtgg tttacgttca atcatagaag aatcgttaat
    19801 cgatattatg tttgatgtgc cttctaacga aaatgtaacg aaggtagtta ttacagcaca
    19861 aacaattaat gaagaaactg aaccagaact atacgacgca gaaggcaatt taattaataa
    19921 tagtaaaaca tcagcttaat cattgatgtg ttataaaagt gaaaagctcc aatcaaagtt
    19981 gacgctcaaa tagtttaact ttgatcggag cttttttata atgtataccg aaaggttcaa
    20041 atattatata atggtagagt tgaaagagaa tataaaaaag tgaggatata aaatgaaagt
    20101 taatcctaat aatatagaat taatcattag tgcagtaaaa gaagaacaat atccagaaac
    20161 agaattgtct gaagttgcac tgagcggtcg atctaatgta ggtaagtcta catttatcaa
    20221 tagtatgatt ggcagaaaaa atatggcacg tacatcacag caacccggca aaacgcaaac
    20281 gttaaatttt tataatatag atgaacaact tatttttgtg gatgttccag ggtatggata
    20341 tgctaaagta agtaaaacac aacgtgaaaa atttgggaaa atgattgagg aatatataac
    20401 taagagagag aatttgcaat tagttattca attagttgat ttaagacatg atccaacaca
    20461 agatgatatc ttaatgtaca attatttgaa acattttgat attcctactt tagttatatg
    20521 cactaaagaa gacaaaattc caaaaggtaa agttcaaaag catattaaaa atattaagac
    20581 acaattagat atggacccag acgatacaat tgtaagttat tcatcaattc aaaataataa
    20641 acaacaacaa atatggaatt taattgaacc gtatatttca tagtttttgt acgacaaaac
    20701 ttatacaaaa attttaaaaa taatgtaagc acgaaacttt taattagtac acaattgata
    20761 acatttttca acgttcatca ttttgtcaaa aactcaaaag taaattagaa agattataat
    20821 ttatttaagc atcgtactta attggatttt aaattatgtt ataatatttg tattgttagt
    20881 atatatgggg gcgtttcaaa tgcattttat tgcaattagt ataaatcatc gcacagctga
    20941 tgttgcacta agagagcaag ttgcttttag agatgatgcc ttacgaattg cccatgaaga
    21001 tttatatgaa actaaatcta ttttagaaaa tgtcatatta tcaacatgta atcgaactga
    21061 agtatatgct gttgttgatc aaattcacac aggtcgttac tatattcaac gatttctagc
    21121 tcgtgcattt ggatttgaag tagatgatat taaagcaatg tcagaagtaa aagtggggga
    21181 cgaagcagta gaacatttat tgcgtgtcac ttctggttta gattcaatcg tacttggaga
    21241 aactcaaatt ttaggtcaaa taagagatgc atttttctta gcgcaaagca caggtacgac
    21301 aggaacaatt tttaatcatc tatttaaaca ggcaattact tttgcaaaaa gagcacataa
    21361 tgaaacagat atagctgata atgctgtaag tgtgtcttat gctgcggtcg agttggcgaa
    21421 aaaagtattt ggcaaattga aaagtaagca agctatcatt attggtgcag gggaaatgag
    21481 tgaattatca ctattaaatc ttcttggttc tggaattact gatattacag tagtaaatag
    21541 aacaattgaa aatgctatga aattagcagc aaagcatcaa gtgaaatatg atgaactatc
    21601 atcattacca aatttacttg aaagtgcaga tattgtgatt agttcaacga gtgcacaatc
    21661 ttatatcatt acaaatgaaa tgatagaaag aattgcagaa aatagaaagc aagattcact
    21721 agtattgatt gatattgcag ttcctcgaga tattgaacct ggtattagtg ccatcacaaa
    21781 catctttaat tatgatgttg atgacttaaa aggtttagtt gatgcaaact tacgtgagcg
    21841 acaattagcg gctgcaacaa tttcggaaca aattcctgca gaaatacatg cacacaatga
    21901 gtggattagt atgttgggtg ttgttccagt gattagagct ttacgtgaaa aagctatggc
    21961 aattcaagca gaaacgatgg acagtattga tcgtaaattg ccagggttaa gcgaaagaga
    22021 gcgtaaaatt atatctaaac atacaaaaag tatcatcaat caaatgttga aagatcctat
    22081 taaacaggcc aaagaattaa gtagtgataa gaaaagtaat gaaaaattag agctatttca
    22141 aaatatattt gatatcgaag ctgaatgtcc tcatgagcaa gcgaagcaac aaaaagaaag
    22201 caaggtcaag gaaatttcag cgcgacgtat ctttagtttt gaataagcat attaatatgg
    22261 tgataata
//
LOCUS       DBNENW010000031        22207 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.031, whole genome shotgun sequence.
ACCESSION   DBNENW010000031 DBNENW010000000
VERSION     DBNENW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.031"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            168..1538
                     /locus_tag="AC1LW8_000931"
     CDS             168..1538
                     /locus_tag="AC1LW8_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0046942 - carboxylic acid transport
                     [Evidence IEA];
                     GO_process: GO:1905039 - carboxylic acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HIP4772785.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLIIMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILIMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            1784..3634
                     /gene="nrdD"
                     /locus_tag="AC1LW8_000932"
     CDS             1784..3634
                     /gene="nrdD"
                     /locus_tag="AC1LW8_000932"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIP4772786.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            3631..4167
                     /gene="nrdG"
                     /locus_tag="AC1LW8_000933"
     CDS             3631..4167
                     /gene="nrdG"
                     /locus_tag="AC1LW8_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIP4772787.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNKKTIWVWTGFLYEYLANDCAERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            4267..4377
                     /locus_tag="AC1LW8_000934"
     CDS             4267..4377
                     /locus_tag="AC1LW8_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772788.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(4393..4554)
                     /locus_tag="AC1LW8_000935"
     CDS             complement(4393..4554)
                     /locus_tag="AC1LW8_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772789.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKTIVLSYDLTNLTNFTRTAFKNIY
                     TLLTNIYLL"
     gene            4686..6308
                     /locus_tag="AC1LW8_000936"
     CDS             4686..6308
                     /locus_tag="AC1LW8_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIP4772790.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            6567..7253
                     /locus_tag="AC1LW8_000937"
     CDS             6567..7253
                     /locus_tag="AC1LW8_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP4772791.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(7441..8004)
                     /gene="cudC"
                     /locus_tag="AC1LW8_000938"
                     /gene_synonym="betI"
     CDS             complement(7441..8004)
                     /gene="cudC"
                     /locus_tag="AC1LW8_000938"
                     /gene_synonym="betI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline uptake/conversion transcriptional
                     regulator CudC"
                     /protein_id="HIP4772792.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(8093..8188)
                     /locus_tag="AC1LW8_000939"
     CDS             complement(8093..8188)
                     /locus_tag="AC1LW8_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772793.1"
                     /translation="MKNVSIYKTTQELSFVSLLDFYLTKHLTLML"
     gene            8257..9747
                     /gene="betB"
                     /locus_tag="AC1LW8_000940"
     CDS             8257..9747
                     /gene="betB"
                     /locus_tag="AC1LW8_000940"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HIP4772794.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            10008..11717
                     /gene="betA"
                     /locus_tag="AC1LW8_000941"
     CDS             10008..11717
                     /gene="betA"
                     /locus_tag="AC1LW8_000941"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIP4772795.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDAKEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            11995..12207
                     /locus_tag="AC1LW8_000942"
     CDS             11995..12207
                     /locus_tag="AC1LW8_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HIP4772796.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            12487..12930
                     /locus_tag="AC1LW8_000943"
     CDS             12487..12930
                     /locus_tag="AC1LW8_000943"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIP4772797.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGYKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            13124..14722
                     /gene="mbcS"
                     /locus_tag="AC1LW8_000944"
     CDS             13124..14722
                     /gene="mbcS"
                     /locus_tag="AC1LW8_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase MbcS"
                     /protein_id="HIP4772798.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(14782..15111)
                     /locus_tag="AC1LW8_000945"
     CDS             complement(14782..15111)
                     /locus_tag="AC1LW8_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772799.1"
                     /translation="MDYSILNLYSILIQYKCKINHKKEIQYLHPRKTSQLPLILKTIL
                     NLSLIGIVIIVFTHAEFQLNAGDSNVFLLTWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            15407..16903
                     /gene="lqo"
                     /locus_tag="AC1LW8_000946"
     CDS             15407..16903
                     /gene="lqo"
                     /locus_tag="AC1LW8_000946"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase (quinone)"
                     /protein_id="HIP4772800.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(17097..17987)
                     /locus_tag="AC1LW8_000947"
     CDS             complement(17097..17987)
                     /locus_tag="AC1LW8_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HIP4772801.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDNLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(18110..18526)
                     /locus_tag="AC1LW8_000948"
     CDS             complement(18110..18526)
                     /locus_tag="AC1LW8_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772802.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPFILEIICHLLIGSLIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            18784..20121
                     /locus_tag="AC1LW8_000949"
     CDS             18784..20121
                     /locus_tag="AC1LW8_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIP4772803.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEITPGDF
                     EKRVTFDLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDGSAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(20201..21649)
                     /locus_tag="AC1LW8_000950"
     CDS             complement(20201..21649)
                     /locus_tag="AC1LW8_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIP4772804.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
BASE COUNT         7684 a         3392 c         4186 g         6945 t
ORIGIN      
        1 cattagagat actgcataaa aattagataa ccgaattaac tattacgcaa aaaagatttt
       61 tttgataaaa tgagataact taaatataaa aaattatatt aattataata tttaagttaa
      121 agagggggat tatgtaaatt gtattaaaag tggagggaga aaataatatg aatagtgata
      181 atatgtggtt aacagtaatg gggctcatta ttattatttc aattgtaggt ttactcattg
      241 ccaaaaagat aaatccagtt gttggtatga caatcatacc ttgcttaggt gcaatgattt
      301 taggatatag tgtgacagat ttggttggat tttttgctaa agggttagat caagtcatca
      361 acgttgttat tatgtttatc tttgctatta ttttctttgg catcatgaac gatagtggtt
      421 tattcaagcc gcttgtcaaa cgcttaataa taatgacacg aggcaatgtc gtcattgtct
      481 gtgcaatgac agctttaatt ggcacaatag cccaattaga tggggccggt gcggtaacat
      541 ttttgctttc tattcctgca ttattacctt tatataaagc gttaaatatg aataaatatt
      601 tattgatttt actattagca ttaagcgcgg cgattatgaa catggtacct tggggaggtc
      661 caatggcacg tgtagctgca gtgttaaaag ccaaaagtgt caatgaatta tggtatggat
      721 taattcctat tcaaataata ggattcattc ttattatgtt gtttgcggta tatcttggat
      781 ttaaagaaca gaaacgtatc aaaaaagcaa tagagagaaa tgaattaccg caaacacaag
      841 atatagatgt acataaatta gttgaagtat atgaacgaga tcaagatgta aggtttcctg
      901 taaaaggacg tgcaagaaca aaatcatgga taaaatgggt gaatacagct ttaactttag
      961 ctgttattct atcgatgtta ataaatattg cgccacctga atttgcattc atgataggtg
     1021 tctcattggc gctcgttatt aattttaaat cagtggatga acaaatggag cgattaagag
     1081 cgcatgcgcc gaatgcatta atgatggctg cagtgattat tgcagcaggt atgtttttag
     1141 gtgtactaaa tgaaaccggt atgcttaaag cgattgcgac gaatttaatc aaagtgattc
     1201 ctgcagaagt aggaccatac ttgcatatta ttgtaggttt acttggcgta ccattagatt
     1261 tactaactag cacagacgct tattattttg ctgtgttacc gatcgtcgaa caaacagcag
     1321 ggcaatttgg tgtaccgtct gtatcaacag cttattcaat ggttataggg aatattatag
     1381 gtacatttgt cagtccgttt tcaccagcct tatggttggc aattggttta gcagaggcaa
     1441 acatgggcac gtatattaag tatgcattct tttggatttg gggattcgcc atcgttatgt
     1501 tagtaattgc aatgttgatg ggcattgtga cgatttaagt atgaaaaata gaaactatag
     1561 tcccggtgca aaatgaaata atagttgcat aaacatgtcg aaatgacaga acgattcgag
     1621 aacaatttta aaaattaatg aaataattgt gtagaaatat gaatttcact aaatgttaat
     1681 aactttgtga cgttttagtt aacagactaa taaaaatttg aaaatactat atatagtggt
     1741 ataacgtaat gagtagacac aatatatagg aagaaggggt aaaatgaatc aaatcgaaga
     1801 agcattaacg ggtttgattt ctaaagatcc tgctattgtt aacgaaaatg ccaacaaaga
     1861 tagtgataca ttttcaacaa tgagagattt aacagcaggt atcgtttcta aatcttacgc
     1921 attaaattat ttattaccaa agcacgttgc agatgcacat caaagagggg acatacattt
     1981 tcacgactta gattatcatc cattccaacc gttaacaaac tgttgtttaa tagatgctaa
     2041 aaatatgcta cataatggat ttgaaatagg caacgcgaat gtaacttcac caaaatcaat
     2101 acaaactgca tcagcgcagc ttgtacaaat tatagccaat gtttccagca gtcaatatgg
     2161 tggctgtacg gttgaccgcg ttgacgaatt acttagtaca tatgcacgac ataatgaaga
     2221 acaacataga aatatcgcaa agcaatttgt caaagaatct gaaattgatc gttatgttga
     2281 tcaacaagtc actaaagaca tcaatgatgc gattgaaagt ctagaatatg aaattaatac
     2341 cttatataca tctaatggac agacaccttt tgtaacatta ggattcggct taggtacaga
     2401 tcatttaagt cgcaaaattc aacaagctat cttaaatact cgtatcaaag gcttaggaaa
     2461 agaccgcacg acagcgattt tcccgaaact tgtattttca attaaaaaag gaaccaactt
     2521 tagtccgcaa gatccgaact atgacattaa acaactagca ttaaagtgtt caacgaaacg
     2581 tatgtatcca gatattttaa attatgacaa actcgtagaa atattaggtg atttcaaagc
     2641 gccaatgggt tgtcgttcat ttttaccaag ttggaaagat gcggaaggtc attttgaaaa
     2701 taatggtcgt tgtaatcttg gtgttgttac acttaattta cctagaatgg cattagaatc
     2761 tgccggtaat atgacgaaat tctgggaaat cttttatgaa cgtatcgatg tgttacatga
     2821 tgcattactt tatcgtataa atcgtttgaa agatgctgta ccgaataacg caccgatttt
     2881 atataaaagt ggcgcattta actataaatt aaaagaaaca gatgatgttg ctgagttatt
     2941 taaaaataaa cgtgcaacga tttcaatggg ctatataggg ttgtatgaaa cagctactgt
     3001 tttctatggt ccagactggg aaacatctca agaagcaaaa gcatttacgc ttgaaattct
     3061 taaagaaatg aaacgttatc aaacgaaatg gacagaatta tatgacattt ggttcagtat
     3121 ttacagtacg ccgagtgaat cgctaacgga tcgtttttgt cgtttagacc aagagagatt
     3181 tggagatatt aaagacatta cagataaagg atattatcaa aactctttcc attatgatgt
     3241 acgtaaagat gttacacctt ttgaaaagtt agattttgaa aaagattatc cttattatgc
     3301 gagtggtggt ttcattcact attgtgagta tccgaaattg caacacaatt tgaaagcact
     3361 agaagcggta tgggactact cttatgacaa agttggttac ttaggtacaa atattccgat
     3421 tgatcattgt tatgaatgtg attacgatgg agattttgaa gcaactgaaa aaggatttaa
     3481 atgcccgaac tgtggcaatg ataatcctaa aacagttgat gtcgttaaac gaacatgtgg
     3541 ttacttaggc aatccagttc aacgtccagt aattaaaggc cgtcataaag aaatttgcgc
     3601 acgagtaaaa catatgaaag cgcctaaaga atgacacttt tagacattaa acaaggacaa
     3661 ggttatattg ctaaaataga atcaaatagc tttgttgacg gtgaaggagt aagatgcagt
     3721 gtttatgtat caggatgtcc atttaattgt gttggatgtt ataacaaagc ctcacaaaag
     3781 ttcagatatg gcgagaaata cactgatgaa atattagcag aaatattaga tgattgcgat
     3841 catgattata tatctgggct aagtctatta ggtggcgaac cattttgtaa tttggatatt
     3901 acattaaatc ttgtcaaagc atttcgagca cgttttggaa ataaaaagac aatttgggta
     3961 tggactggat ttttatatga atatttagca aatgattgtg cagaacgtcg agagttatta
     4021 tcatacattg acgttttagt agatggtcta tttatacaac acttattcaa acctgattta
     4081 ccatataaag gttctttaaa tcaacgcatt atagatgtac aacaatcact ctcgcatgcg
     4141 cgtatgattg aatatatagt tagttgaata tgtattagaa gtcaaggtaa cattcgttgc
     4201 cttggcttct ttttaggtta ggtacataat tgaaagttaa taaaagcaat tctttagaaa
     4261 aatatattga tagaatatga cctaacaatc attttgatac caatactaaa agttgcatat
     4321 ccgtttttta aaaaagttga aagagaaaag tggtatttta gtgggaagga agtctaactt
     4381 tttggtagcg ttttacaata aataaatatt cgttaataac gtataaatat tcttaaatgc
     4441 cgtcctagta aaatttgtta aattcgttaa atcataactt aacactattg ttttagcgct
     4501 attaaggttt tgtttattac gggaaaaatt atataaatat tcaataattg ccaagtttca
     4561 aattgtatga aatttgcatt attattaaat gttagttatt gtcaattttg tgaatcaata
     4621 taattattac attttgagat aaatcgaaac aggattcata aaattaataa ttagggggag
     4681 cacaattgaa aaaagagaaa gttatggact ggacgacctt tatagggaca gtagctgtac
     4741 ttctttttgc agttatacct atgatggctt ttccaaaagc aagtgaagat atcatcactg
     4801 gtattaatag tgccatttct gattcaattg gttcgatata tttatttatg gggctggcga
     4861 ttttttgctt tgtaatgtat atcgcatttg gtaaatatgg taatgtcaca cttggtaaag
     4921 caagtgataa accagaattt aatacattta catgggcggc aatgctgttt tgtgcaggca
     4981 taggctctga tattttatac tggggcgtta ttgaatgggc tttttactat caagttccac
     5041 caaatggcgc gaaaagtatg agtgatgaag cactccaata tgcgacgcaa tatggtatgt
     5101 tccactgggg gccaattgct tgggctattt acgttttacc agcattacca attggttatt
     5161 tagtatttgt taaaaaacaa ccggtgtata aaattagtca agcttgtcgt ccgattttaa
     5221 aaggtcaaac agataaattt gtaggtaaag ttgtagatat cttatttatc tttggattgc
     5281 taggtggtgc ggcaacatca ctagcgttag gtgtgccatt aatttctgca ggcatagaaa
     5341 gattaactgg tttagatggt aaaaatatga ttttacgttc ggccatttta ttaacaatca
     5401 cggttatatt tgccattagt tcatatacag gattgaaaaa aggtattcaa aagttaagtg
     5461 atatcaacgt ttggctatcc tttgtacttt tagcctttat atttattatt ggaccgactg
     5521 tttttattat ggaaacgaca gtgacagggt tcggaaatat gttgagagat tttttccata
     5581 tggcaacatg gttagaacca ttcggtggta ttaaaggtcg aaaagaaacg aatttcccac
     5641 aagactggac aatattctac tggtcatggt ggttagtata tgcgccattt atcggtttat
     5701 ttatcgctag aatttcaaaa ggtcgacgcc ttaaagaagt cgtgctagga acaattattt
     5761 atggaacgct tggatgcgta ttattctttg gtatttttgg taactatgct gtgtatttgc
     5821 aaatttctgg acagtttaat gtaacacaat atttaaatac acatggtaca gaggcaacca
     5881 ttattgaagt ggtgcatcat ttaccattcc catcattgat gattgtacta ttcttagtat
     5941 ctgctttctt attcttagca acaacatttg attcgggttc atatatttta gcggcagcat
     6001 ctcagaaaaa agtggtaggc gaaccattac gtgccaatcg tttattctgg gcatttgcat
     6061 tgtgcttatt gccattttca ttgatgctag ttggtggcga acgtgcatta gaagtattga
     6121 aaactgcttc aatactggca agtgtgccat taattgttat ttttattttc atgatgatat
     6181 catttttaat cattttaggg cgcgatagaa ttaaacttga aacgcgtgct gaaaaattaa
     6241 aagaagttga acgtcgttca ttgcgaatcg ttcaagtatc agaagaagaa caagacgata
     6301 atttataatt caaagcgggt ctgggacgac gaaatgaatt ttgtgaaaat atcatttctg
     6361 ttccattccc ctttttttag tagcattgta ggatgaactt ttaggttttc attaatgttg
     6421 tactaaaaga tttaattttt tagtgctcca agtacttatt tattgtatga agcatattct
     6481 aaatcgaagt ttgaaagact ctcattgatt attaaataaa gggtactgcg tatgtacaat
     6541 tcaaattaat cgaaggatga aataaaatga ctaatcaatt taaaaataaa cagtccaaat
     6601 tacatgacag tttagaatcc atcacaaaaa acttatatgc gacacctact tcggaactgc
     6661 cttttgataa tcgatttcta tttaaatctt ttattttaaa acgtgaaaca ggtaatattg
     6721 ttatctatca ttccggacac ttaggtgact cccaacaaga cattgcatca ttaggtggtg
     6781 tttcaaaagt attgatgaat catgatcatg aatctatagg aggttctaat caagttgaag
     6841 ccccttactt tatacatgaa aatgatgtgg ctgcactgaa acataagatt tctgttcaaa
     6901 aacaatttag taatcgtgta atgttggata aggatttaga agttattccc gcgcctggac
     6961 atacaccagg gacgacacta tttttatggg atgatggtca tcaccgttac ttatttactg
     7021 gagattttat atgttttgaa gggaagagat ggcgtacagt tatattaggt tcaagtgata
     7081 gagaaaaatc tattcaaagt ttagagatgg ttaaagaatt agattttgat gtacttgtac
     7141 cttgggttac tatcaaagat gaaccgttag tttattttgt agaaaatgaa tatgaaaaac
     7201 gtgaacaaat acaaaatatt attgatagag tacgtgaggg cgagaatagc taattgaaat
     7261 aaattggcga agcaatgtaa cgaatctaag aaagctctag aaaatacctc catgattgat
     7321 tgtcatataa aacaaaaacg gtaatttcta tttattgaga tagaaattac cgtttatttc
     7381 gtggacctat tgcattgttt tcatcatgca taatcatcat tgtcgttgtt tgagtcaatt
     7441 ttaattttca gaatcagaag gctgttctgg aattgggaaa tatttgaaaa tttcaccgct
     7501 ttcaatcgct tcggttaact gttctaacca ttcgtaataa acatgtgtat gatcaagctg
     7561 agctttaatt ttttgtgcct cttgtgtttc agcttcagtt aaatcactgc tttcaagtaa
     7621 tggattgata atagcttgag catcttttac tgcttcgaca ttgatgtcaa tttcacgctg
     7681 aaatttttta gtgaaaaagt ttcggaaaaa gatgaaaaag tctttctcgg cgataaaatg
     7741 ttgtttgcgg cttcctctcg taaattgttg tttaacaata tcaaattcct gcaatttctt
     7801 aacgccagca ctcatacttg gtttgctcat ttgcaattga tgacgcattt catcaagcgt
     7861 catactgcct tcaaacacca tagtgccata taagtttcct acacttctat tagtgccata
     7921 caaatccatt gtctgtccaa ttgaattaat tacaatatct tttgcttgtt ctaattgttg
     7981 ctgtttgttc tgagaacgag tcatcattgc acctccgtac atcattttgg tcattctaaa
     8041 ataaatacta atacattata aaaccttttc taaaaaaaga cattaaaaat atttaaagca
     8101 ttaaagttaa atgtttcgtt aaataaaaat ctaacaaact tacaaaactt aattcttgag
     8161 ttgttttgta aattgacaca tttttcattt ctatgctaac ataagtttgt aaaattcgtt
     8221 aaataaaaat ttaacaaact taacggaggt tgttgaatgg aacttttaaa acatttatct
     8281 cagcgtcaat atattgatgg tgagtgggtt gaaagcgcga ataaaaatac aagagatatt
     8341 atcaatcctt acaatcaaga agtgatattt acggtttctg aagggacaaa agaggatgca
     8401 gaacgtgcaa tcttagctgc aagacgtgcg tttgagtctg gtgaatggtc gcaagaaact
     8461 gctgaaacaa gaggtaaaaa agtacgtgcc atcgcggaca agattaaaga acatcgcgaa
     8521 gcgttagcac gattagaaac attagatact ggaaaaacgt tagaagaatc atatgcagat
     8581 atggatgata ttcataatgt gtttatgtat tttgctggat tagcagataa agacggtggc
     8641 gaaatgattg attcaccaat tccagataca gaaagcaaaa ttgttaaaga accagtaggt
     8701 gtagttacac aaattacacc ttggaattat ccgttattac aagcatcatg gaaaattgcg
     8761 ccagcgcttg ctacgggttg ttcactagtt atgaaaccaa gtgaaattac accattaaca
     8821 acaatacgtg tttttgaatt aatggaagaa gttggtttcc ctaaaggaac aattaatctt
     8881 attctaggtg caggttctga agttggtgac gtaatgtcag gtcataaaga ggttgacctt
     8941 gtatcattta caggtggcat tgagactggt aagcatatta tgaaaaatgc tgctaataat
     9001 gttacgaata ttgccttgga acttggcggt aaaaatccaa acattatctt tgatgatgct
     9061 gattttgaat tggcagtaga ccaagcgtta aatggtggat atttccatgc aggtcaagtt
     9121 tgttcagcag gatcaagaat attagtacaa aacagtatta aagacaaatt tgagcaagca
     9181 cttattgatc gcgtgaaaaa aatcaaatta ggtaatggtt ttgatgctga tactgaaatg
     9241 ggaccagtga tttcaacaga acatcgtaat aagatcgaat cttatatgga tgtagctaaa
     9301 gcagaaggcg caacaattgc tgttggtggt aaacgtccag atagagacga tttaaaagat
     9361 ggtctattct tcgagccaac agtcattaca aattgtgata cgtcaatgcg tattgtacaa
     9421 gaagaggttt tcggacctgt cgttactgta gaaggctttg aaactgaaca agaagcgatt
     9481 caattagcga atgattctat atatggttta gcaggtgctg tattttctaa agatattgga
     9541 aaagcgcaac gcgttgctaa caagttgaaa cttggaacgg tgtggattaa tgatttccat
     9601 ccatattttg cacaagcgcc atggggtgga tacaaacaat caggtatcgg tagagaatta
     9661 ggcaaagaag gcttagaaga gtaccttgtt tcaaaacaca ttttaacaaa tacaaatcca
     9721 caattagtga attggtttag caaataaaaa ttagataagg tgagtgccat tgtaagaaca
     9781 caagacactc actttgtttt gtataagtgg cgaaatgttg attgataatt tggactaaac
     9841 gcaaaatgaa tcatagatta tttcatgact gttagtaaca atcgtaaaag gaaaatcgag
     9901 tgttttggtt agctaagttt agcaattcaa cgataaccaa tcagccacta acaaatattt
     9961 catgcaatac tcactttgaa atacaacaaa ctttggaggt cataacgatg agtaacaaaa
    10021 acaaatcata tgattatgtc atcattggag gaggcagtgc aggttctgta ctaggtaatc
    10081 gtctgagtga agataaagat aaagaagtct tagtattaga agcgggtcgc agtgattatt
    10141 tttgggattt atttatccaa atgcctgctg cgttaatgtt cccttcaggc aataaatttt
    10201 acgattggat ttattcaaca gatgaagaac cacatatggg cggtcgtaaa gtagcacatg
    10261 cccgaggtaa agttttaggt ggatcaagtt cgattaatgg catgatttac caacgtggta
    10321 atccaatgga ctatgaaggc tgggcagaac cagaaggtat ggaaacttgg gattttgcac
    10381 actgtttacc gtattttaaa aaattagaaa aaacatacgg tgcagcgcct tatgataaat
    10441 ttagaggcca tgatggacca attaagttaa aacgagggcc agcaacgaat cctttattcc
    10501 agtcattctt tgatgcaggt gttgaagcag gctatcataa aacacctgat gtgaatggat
    10561 ttagacaaga aggttttgga ccgttcgata gtcaagtaca tcgtggtcgc cgaatgtcag
    10621 cttcaagagc atatttacat ccagcgatga agcgtaaaaa cttaaccgtt gaaacacgtg
    10681 cctttgtaac tgaaattcat tatgaaggta gaagagcaac tggtgttacg tataagaaaa
    10741 atggcaaact acataccatc gatgctaaag aagtcatttt gtctggtggg gcattcaata
    10801 cgccacaatt actacaatta tctggtatag gtgattcaga gttcctaaaa tcaaaaggca
    10861 ttgagccacg tgttcattta cctggtgtgg gtgaaaactt tgaagatcac ttagaggtat
    10921 acattcaaca taaatgtaag gaacctgtat cattacagcc aagcttagat atcaagcgca
    10981 tgccatttat tggtttacaa tggattttca cacgtacagg tgcagcagca tctaaccatt
    11041 ttgaaggtgg cggatttgta cgttcaaata acgaagttga ttatcctaac ttaatgttcc
    11101 atttcttacc aatagctgta agatatgatg gtcaaaaggc agcggtcgca catggttatc
    11161 aagttcacgt aggaccaatg tattcaaact ctcgtggtag cttgaaaatc aaatctaaag
    11221 atccattcga aaaaccaagt atccgcttta attatttatc aacagaagaa gataaaaaag
    11281 aatgggtaga agcaattcgt gtagcaagaa atatcttatc tcaaaaagca atggatccat
    11341 tcaatggtgg agaaatttca ccaggaccag aagtgcaaac agacgaagaa attcttgatt
    11401 gggttcgtag agatggtgaa acagcattac acccatcttg tagtgctaaa atgggtcctg
    11461 cttcagatcc aatggcagta gttgacccgt taacaatgaa agtccacggt atggaaaatt
    11521 tacgtgtagt tgatgcatcc gctatgccac gtacgacaaa tggcaatata cacgcacctg
    11581 ttttaatgtt agctgaaaaa gcagcagaca ttatacgtgg cagaaagcct ttagagccac
    11641 aatatattga ttattataag catggtgttc atgatgaaaa tgaaggtgca atcgaagtaa
    11701 aaccatacgc taaataaatt tatatagtag gctcttagtc tggcatctat tgtattggtg
    11761 ccaggctttt ttgcgtttta tctaaatgag aagttaatgt gttgttcaaa aacggtgtgt
    11821 gagtatatga actgaaaata gagttaattg tacattgtga tgattaattg acctaattgg
    11881 cagacattaa aagagtgcac aaccttaaaa agttcactaa atagacattt aattgtgttt
    11941 tcttgaaagc tttcgtggta ggatttatat aagtaaattg agagaaggaa tactatgagc
    12001 ttttacgaat atatacaaac atttaaagat gataaaacac cattaggcga attagcgatt
    12061 tggattaaag aagatgattc attcccaaaa caagagaagc taacagaaaa cattttgtct
    12121 tattttcatc aaatgtccaa tatagatcat gagtttttag aaattgtaaa aagatcactt
    12181 tctctgtatg atcaattaaa atcgtaaaat cagaaataaa gttagctaaa ataaaaggcc
    12241 ggtgatttca cagatgaagt tgtcggctat tttcatgact aatgttaaat aatgatcatg
    12301 aaattgaaga cacaaaaata gtgggcgact cctgtcccca tttgcatgaa atattaatac
    12361 tttttacaaa gtcttatctt ttaaaaagaa gccaaactat gattgtaatt aataaatacg
    12421 ggaatgaatt agtaatagat ataaagtaat atgaccaaaa atataaatga aatgaaggca
    12481 tttattatga acatttatga tgaatataga agttatgtaa tagaagaact ggatgattgt
    12541 ctcacaatac aaaaaaataa tgacactgca tattacgatg ttttggaagc gataaatgat
    12601 ttatcaaatg attcattatg tgtgttaaat catttatata ttaacgaagg acaagaagaa
    12661 acattcgagc aaaaattttt gcgacgtaat aagcatttaa aaaacgttga tggatataaa
    12721 gcattaaggt ttttaagacc tagaacagct ggtagacact atatcattat tacgctgtgg
    12781 gaaaataggc aagcatttta tcattggcaa aattcagctg agtacaagca tacacacaag
    12841 catcgtggca cttcgaaagg tgcagatgta aaaataatta atcgtgaatt atcatacaat
    12901 attcgtattg aattagctga tatggtctag cccgagatat caaccctcaa tcatttgaaa
    12961 gtccttgaat gctacgtcat gtcaaagtgt agtgtcgagg actttggtat ttaaccctat
    13021 ttaattgaaa attctgtaaa gataggcgta tgatatatat aattcattta taaaattaca
    13081 atgttcatca caggaaaagt ggatgtatga aaggaagtta gttatgcaaa agtcagctct
    13141 attcgcgcct gaaaaatata acattatttc ggaaatcgaa aaatatagcc acataccaga
    13201 taaaaaggca attttatatc ataatactga aggcgaagat atatcagtca cttaccaaca
    13261 gctcattgaa cagtctaata aagtaggtaa tgttttagca tcgcacggtt tatctaaagg
    13321 cgataaagta ctcattatga tgccacgatc tattgcgacg tatgaattat atattgctgc
    13381 tttaaaatta ggtgttgcaa tcattccatg ttctgaaatg ctaagaacta aagatttaca
    13441 ataccgtatc acgcacggtg aaataaatgc agttattgca ctagagccat tcacagttga
    13501 atttgaaaaa attaaagaat atgatgcgtt aactaaattt gttattgccg gtcaaaaaga
    13561 tggatggatt tcacttgaag ctgaaaaaga aaacgcaagt aacaaactag aaatggcaga
    13621 tacgacgcgt gatgatatgg cgattctgtc atatacatct ggtacaactg gcaatcctaa
    13681 agccgttaca cattgtcatg gatggggatt tgcacattta caaatggcac caaaacattg
    13741 gttatgtatt aatgaagatg acttagtttg ggcaacagca gcaccaggtt ggcaaaagtg
    13801 ggtatggagt cctttcttat ctgtattagg ttcaggcgca actgcttttg tatttaatgg
    13861 aagattcagt cctgaaacat acttagaatt gcttcaaaaa tatcaaatta atgttttatg
    13921 ttgtacaccg acagaatatc gtatgatggc taaattacag aacttaaatg attatgactt
    13981 aacgcattta catagtgccg tttctgcagg tgagccacta aatcgagaag tagttgaaca
    14041 attcaagaaa tactttaatt taactgtaag agatggatat ggccagactg aaagtacctt
    14101 gcttataggc tttttaaaag atacaccaca gaggattgga tcaatgggta aaggcattcc
    14161 aggtagttcg gtcactgtag tagatgatga ggggaatagc gtacctgcta atacgaaagg
    14221 gaatattgca gtaccacttg atttgccagc actttttaaa gggtattata aagagccaga
    14281 acgtacggcg aaagcgcaaa caggtaaata ctacatcact ggtgacttag ctcatgtaga
    14341 tgatgatggt tatttctggt ttgaaggacg tcgagatgac attattatta gttcaggcta
    14401 tacgattggg ccttttgaag ttgaagatgc gttaacgaat catccagccg ttaaagaatg
    14461 tgctgtagta gcaaagcctc atgatatacg aggaaatatt gttaaagcat tcgttatttt
    14521 acaggaccat actgcgggtg atgatacgtt agttaaagaa ctacaacaat ttgttaaaaa
    14581 tgaagttgca ccatataaat atccgagaga gattgaattc gttgacgact taccaaaaac
    14641 aaactcaggt aaaattcgtc gtgttgaact acgagattct gaaatagaaa aatggcaaca
    14701 acaaaaagat agcaatcaat aaacatagca aagcagtcca atgcgtaagg tatgatgata
    14761 cgcactggac tgcttgttta tttatgatgt tctttgtcgt ctagttataa atattaaaat
    14821 tagtttagaa acaagcgcaa ccagtattaa gaatgagaaa atataagatg ttaaacctcg
    14881 aatatcccat gttaataaaa acacattact atcaccggca ttaagttgaa actcagcatg
    14941 tgtaaaaacg ataatcacaa tgcctataag tgaaagattc aaaatagttt tcaatattaa
    15001 aggtaattgt gatgtttttc ttggatgcag atattgaatc tcctttttat gattgatttt
    15061 acacttatat tgtatcaaaa tagagtatag gtttaaaatg ctataatcca aatgtagatt
    15121 aaaacaatcg ttagctattt gttgtcaaat aattatgatc gatttgatat ttatcttcta
    15181 tgaataaaag atttaatata ttgcaaagtt aatgaataat tataatttac atttaccact
    15241 gcttgcttta acatatacat gtaagcacta actatagtat tttgttacag cgtatcaatt
    15301 aaaacaggaa gttatctatg gcgttgatat tttaaagaaa tatgtcagtt ttatgtaact
    15361 ttcatattga tgatacacat ttacaatttt tggagaggtg aaaccaatgg ctaagtctaa
    15421 tagtaaagac atcgttttaa ttggagccgg tgtacttagc acaacatttg gttcaatgtt
    15481 aaaagaaatt gagccagact ggaatatcca cgtttacgaa cgcttggatc gtcctgcaat
    15541 cgaaagttca aacgaaagaa ataatgctgg tacgggtcat gcagcattat gtgagttgaa
    15601 ctacacagtt ttacaacctg atggttctat cgacatcgaa aaagcgaaag tgattaacga
    15661 agagtttgag atttcaaaac aattctgggg tcacttagtg aaaagcggta gcatcgagaa
    15721 cccaagagaa tttatcaatc cattaccaca catcagttat gttagaggta aaaacaatgt
    15781 taaattctta aaagatcgtt acgaagcgat gaaagctttc cctatgttcg ataatatcga
    15841 atatactgaa gacatcgaag taatgaaaaa atggattcca ttgatgatga aaggccgtga
    15901 agataaccct ggtatcatgg cggcaagtaa aattgacgaa ggtacagatg taaacttcgg
    15961 tgaattaaca cgtaaaatgg ctaaaagcat tgaagcacat ccaaatgcta cagtgcaatt
    16021 taaccatgaa gttgttgatt ttgaacaatt atcaaatggt caatgggaag ttactgttaa
    16081 aaatcgccta actggtgaga aattcaaaca agtaactgac tatgtattca tcggtgctgg
    16141 cggtggagcg attccattat tacaaaaaac aggtatccct gaaagtaaac atttgggtgg
    16201 attccctatc agtggtcaat tcttagcttg tacaaaccca caagttattg aacaacacga
    16261 tgccaaagtt tatggtaaag agccacctgg tacaccacca atgactgtac ctcatttaga
    16321 tacgcgttac attgacggcc aaagaacatt gttatttgga ccatttgcta atgttggacc
    16381 taaattcttg aaaaatggtt ctaacttaga tttattcaag tctgttaaaa catacaacat
    16441 tacaacttta ttagcagcag cagttaaaaa cttaccttta attaaatact catttgacca
    16501 agtaattatg acaaaagaag gttgtatgaa ccacttacgt actttctatc cagaagcacg
    16561 taatgaagat tggcaattat acactgctgg taaacgtgta caagttatca aagatacacc
    16621 tgaacacggt aaaggattca tccaattcgg tacagaagtg gttaactcac aagaccacac
    16681 tgtaatcgca ttattaggtg aatcaccagg ggcttcaact tcagtttcag ttgcgttaga
    16741 agtattagaa cgtaacttcc ctgaatacaa aactgaatgg gcacctaaaa tcaagaaaat
    16801 gattccatca tacggtgaat cattaattga agacgaaaaa ttaatgagaa aaatccgtaa
    16861 acaaacttca aaagacttag aattaggtta ctacgaaaac taaattctaa tagctatatt
    16921 taaaatcggg caactgagaa atcttataga tttttcagct gtccgatttt ttcgcgtaat
    16981 ctataataca tatggcaaaa tcaactttaa tcaaaaagac tcatacacaa tgcctttaaa
    17041 gcacatgtat gagtcctttt agtagtttat atcaaaaaat atgtttaatg tataaattag
    17101 tttttgttta cagatgcgtc gtagattgat tctacagcgt cacctaatgc tttatcgaat
    17161 tcttctttag attgtccagc tcttaaatca ctagctaatg cacgtgagaa gcttgcgata
    17221 agttcagcgt tatctttaag taattcattt gctttttctc tgctgtaacc acctgataat
    17281 acaacgacac gaacaacatt aggatgttca gctaactctt tgtataagtt tggttcagta
    17341 ggaattgtta atttcaacat tactaattga tcagcattta agttatctaa acctttttta
    17401 agttcagctt ttaatacttt ttcaatttca gctttgtctt ttgcattaat attaacttct
    17461 ggttcgataa ttggaactaa acctttagca ataatttgtt tagcaacttc aaattgttgt
    17521 tcaacaacgt ctttgatacc ttgctcattt aattctaaaa tgttagaacg catttttgta
    17581 ccaaaaatgt gacgttcgtt tgcacggtct aataaactgt ctaagttgtc gattggtttc
    17641 attaattgaa caccattttg ctcttcagca agacctttgt ctacttttaa gaacggaaca
    17701 acacctttat cagctaagta atctgcagtg tatttgcctt ctacttcgcg atccattgtt
    17761 tgttcgaaaa gaatagcacc caaaatttta tctggtgaga atgaaggtga agttaccaca
    17821 cgtgtacgca tatcgtgaac aagttggaac atttcgtctt cattgctata ttgatcttcg
    17881 tttacaccat attctttaag tgcttttggt gtactaccac cactttggtc taatgcggca
    17941 ataaagcctt ttccattttt cattttttct aattgctctt tattcatact ttccactcct
    18001 taacttttca atacacctcc agtatgataa aaatgagaac atttctcaag tcataaacct
    18061 tgaaaagtgt ataaaatgtg aaaaataatt gtcagtttaa ttaaaaatat tattttattc
    18121 taggtatgac taacgccatt aatgacataa agaaaatatg tgtaataatc caaccgatta
    18181 attctgtcac actaaattga aaaattggac gttgcgcaat aaatactaaa aagggataca
    18241 atgctataaa taagaaaaat aaagggatat aacatagata gtaaagcctt ttagaagtat
    18301 gaaacagtaa cacaaataca aaataaatga ggctaccaat tagtaagtga catattattt
    18361 ctagaataaa tggtgtattg tcagaaggaa ttagaaaatc taaattcaat aataaagctt
    18421 ttaaatgtga ttgattgctt attaagtcaa acaacatggt catccatagc aacatgaatg
    18481 atatgaaaat agcagcaatg aaatggaaca atacttttaa acgcattcgt atcacctcag
    18541 agtcaaatgg aatatagtaa acaatttcct aatcgaacaa aattatgcgc ataaacaaag
    18601 tagattgata taaaattctt aattatcaga atatatttac aaatctgaaa tttattagta
    18661 tattggatag tattcataga ggcatgacgg tatttgagca gtattttaaa tcggatttta
    18721 taatcgattt aagagaggcc actttgcttg cacattaata ctgtcaatgg gaggggaatg
    18781 tatatgagta aagcacatca attaattcaa gaggatgaac attattttgc gaaatcagga
    18841 cgtattaaat attatccgtt agtgattgat catggatatg gagcaacatt ggttgatatt
    18901 gaggggaaga catatatcga tttgttatcg agtgcgagtt ctcaaaacgt aggtcatgca
    18961 cctagagaag taacagaagc gataaaggca caagttgata aatttattca ttatacacca
    19021 gcatatatgt atcatgaacc gttggtacgt ttagctaaga agctttgtga gattacacct
    19081 ggagattttg aaaaaagagt gaccttcgat ttaaccggat cagacgcaaa tgatggcatc
    19141 attaaatttg ccagagcata tacagggcgt ccttatatca ttagtttcac taatgcatat
    19201 catggttcaa cttttggctc attgtctatg tcagctatta gtttaaatat gcgcaaacat
    19261 tatggtccgt tattgaatgg tttttatcat attccgtttc cagataaata tcgtggtatg
    19321 tacgagcagc cacaagctaa ttcagtagaa gaatatttag cacccttaaa agaaatgttt
    19381 gcgaaatatg tacctgctga cgaagtagca tgtattgtta ttgaaacgat acaaggcgat
    19441 ggtggacttt tagaaccagt tccagggtat tttgaagcgt tagaaaagat ttgtcgtgaa
    19501 catggtattt taatcgctgt cgatgatatt caacaaggtt ttgggcgaac aggtacatgg
    19561 agttcagtct cgcattttaa ttttacgcct gatttaatca cttttggaaa atctttagca
    19621 ggtggtatgc ctatgtcagc aattgttgga cgcaaagaga ttatgaattg tttagaagcg
    19681 ccagcacatt tatttacaac aggtgctaat ccagttagtt gtgaagctgc attagccaca
    19741 attcaaatga ttgaagatca gtcgcttctt caggctagtg cggaaaaagg ggaatatgtt
    19801 aggaaacgaa tggatcaatg ggtatctaaa tacaatagtg taggcgatgt tagaggtaaa
    19861 ggtttgagca ttggtattga tattgtttcc gacaaaaaac tcaaaacacg tgatggcagt
    19921 gcggcactta aaatttgtaa ttactgcttt gagcatggcg tagttattat agctgtagca
    19981 ggaaatgtgt tgcgattcca accgccattg gtaataacat atgagcaatt agacacggcg
    20041 ttaaacacta tagaagatgc actgactgct ttggaagcag gtaacttaga tcaatatgac
    20101 atatctggac aaggttggta atagcgatta tcttaatata aaataaaaaa tcatttccac
    20161 atctggatgt taatcagatg ggaaatgatt tttttgattt ttattttggt gggtggtatt
    20221 cagctacgtc atttttctta gaatgtctaa gtccataact taaatataga atgataccaa
    20281 caataaacca aattaaagtg tataatttcg cttcgaatcc taatccccag aatactagca
    20341 atactaaaac aaatgtaact gctggtaaca caggatataa aggtaattta aatgcaggaa
    20401 ttggtagatc tttaccttca cgctttctca aacgatacat tgctaatgaa acgaacataa
    20461 atgcaacaag tgtacctgct gaaattaatt gtgctaaaaa tgcgaatggg aacatagaac
    20521 caattaaaac accaataata gtaagtataa ctagtgcgcg attaggtaaa tgtttgtcgt
    20581 ttaaatggct taaccatgaa ggtaataagc cgtcacgtcc aaatgaataa agtaaacgtg
    20641 agcctgctaa catcatacca attaatgctg taaacatacc gataacagag atagcttgaa
    20701 caatagctgc tacaacacca tgaccacttt gacgtaaagc ccaaccaaca ggttcagcat
    20761 tgtttgcgta ttgtgagtaa tggaacatac caactaacac aagtgctaca gcaataaata
    20821 atacgatagc aacacttaaa gaaccaagaa tacctctagg cattgtcttt tgagggtcaa
    20881 gtgcttctgc tgagtttgct gcgatagaat cgaaaccgat atacgctaag aaaatcattg
    20941 aaacaccagc atatatgcct tgccaaccac caaagtcacc tgtagcagtt actttgtgtt
    21001 ctggaataaa tggcacatag ttactaacat ttattgctgt taaacctacg atgacaaata
    21061 aaataatagc taatactttt aaaataacta aaatattttc catacgtgct gcttccgaca
    21121 taccacgtga tagtaataat gcagttaata aaataacgat agcagcaata atatcgataa
    21181 aaccgccatt cgtaccaaat ggatttgata atgctgctgg taattcgatg ccaattggtt
    21241 tcacaagtcc gcgtaaattc gctgagaatc ctgatgcaac aaaggctacg gcgataaaat
    21301 attcagctaa tagagcccaa ccggcaaccc atccaaaaaa ttcaccaaat aatacattga
    21361 cccaagaata ggctgaacct gcaaatggca tagcggcagc catttctgca taagtaaatg
    21421 caactaaacc agcaacaata gcagcgagta agaatgataa cgcaacggcc ggtcctgcat
    21481 gttctgcagc aacaatgcca ggtagtgtaa agatagatgt cgatacaatt gttcctacac
    21541 ctaaagctaa gaaatcacgc acccgaagtg tacgctttaa atgaccatct ttattttgat
    21601 agatagccgg atcctctttt cgtgctattt tattgaaaaa acttcccata aactttcctc
    21661 ccaaacattc ataaacaatt ctatacggtg ttttttaata tgttatatca tagcacaaat
    21721 aatcaatatt ttgtctaaaa attctgaaaa atcacaactt tatgttacgt attaatgact
    21781 tgtcttgata acatccatag attttttaaa tgataaaact gattataaca gatattaaat
    21841 gaataagtac tattttttgc taattttcta acaattttgc acattatatt tttaaaatca
    21901 atttcatgtt tatgtctgat tggctagttt gtatgaaatg taattctttg actataaaat
    21961 gatgcatcaa atatgatgag ggtaaggcgg aaaataggag gttatatagt aagtcattta
    22021 agtcgaatag ccatcctttt taaaatgaaa aaaatagaaa gctcaatagt ttgttaaagc
    22081 ctttaaatag cgtcgttacg caattttaga atgctaaaaa ttgtcacaaa tttcaaatta
    22141 cgtgctaaag cttgagatat caatatttat tggcgataga gtgtaatttg actccgctta
    22201 catctca
//
LOCUS       DBNENW010000032        22178 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNENW010000032 DBNENW010000000
VERSION     DBNENW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.032"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(456..1682)
                     /gene="ftsW"
                     /locus_tag="AC1LW8_000951"
     CDS             complement(456..1682)
                     /gene="ftsW"
                     /locus_tag="AC1LW8_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division peptidoglycan polymerase FtsW"
                     /protein_id="HIP4772805.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(1996..2271)
                     /locus_tag="AC1LW8_000952"
     CDS             complement(1996..2271)
                     /locus_tag="AC1LW8_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HIP4772806.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            2412..2894
                     /locus_tag="AC1LW8_000953"
     CDS             2412..2894
                     /locus_tag="AC1LW8_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772807.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            2896..3063
                     /locus_tag="AC1LW8_000954"
     CDS             2896..3063
                     /locus_tag="AC1LW8_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2197 domain-containing protein"
                     /protein_id="HIP4772808.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(3203..5050)
                     /gene="typA"
                     /locus_tag="AC1LW8_000955"
     CDS             complement(3203..5050)
                     /gene="typA"
                     /locus_tag="AC1LW8_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIP4772809.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            5152..5343
                     /locus_tag="AC1LW8_000956"
     CDS             5152..5343
                     /locus_tag="AC1LW8_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5325 family protein"
                     /protein_id="HIP4772810.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(5497..6324)
                     /locus_tag="AC1LW8_000957"
     CDS             complement(5497..6324)
                     /locus_tag="AC1LW8_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIP4772811.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            6481..7095
                     /locus_tag="AC1LW8_000958"
     CDS             6481..7095
                     /locus_tag="AC1LW8_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HIP4772812.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERNFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            7279..8631
                     /locus_tag="AC1LW8_000959"
     CDS             7279..8631
                     /locus_tag="AC1LW8_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIP4772813.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(8713..9132)
                     /locus_tag="AC1LW8_000960"
     CDS             complement(8713..9132)
                     /locus_tag="AC1LW8_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HIP4772814.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(9415..10434)
                     /gene="auxB"
                     /locus_tag="AC1LW8_000961"
     CDS             complement(9415..10434)
                     /gene="auxB"
                     /locus_tag="AC1LW8_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid stability factor AuxB"
                     /protein_id="HIP4772815.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(10508..11581)
                     /locus_tag="AC1LW8_000962"
     CDS             complement(10508..11581)
                     /locus_tag="AC1LW8_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4772816.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            complement(11581..12390)
                     /locus_tag="AC1LW8_000963"
     CDS             complement(11581..12390)
                     /locus_tag="AC1LW8_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772817.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            complement(12396..13193)
                     /locus_tag="AC1LW8_000964"
     CDS             complement(12396..13193)
                     /locus_tag="AC1LW8_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772818.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            complement(13186..14280)
                     /locus_tag="AC1LW8_000965"
     CDS             complement(13186..14280)
                     /locus_tag="AC1LW8_000965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772819.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            complement(14293..14832)
                     /locus_tag="AC1LW8_000966"
     CDS             complement(14293..14832)
                     /locus_tag="AC1LW8_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4772820.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            complement(14976..15251)
                     /locus_tag="AC1LW8_000967"
     CDS             complement(14976..15251)
                     /locus_tag="AC1LW8_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HIP4772821.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(15419..16825)
                     /gene="lpdA"
                     /locus_tag="AC1LW8_000968"
     CDS             complement(15419..16825)
                     /gene="lpdA"
                     /locus_tag="AC1LW8_000968"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIP4772822.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(16829..18121)
                     /locus_tag="AC1LW8_000969"
     CDS             complement(16829..18121)
                     /locus_tag="AC1LW8_000969"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIP4772823.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(18212..19189)
                     /locus_tag="AC1LW8_000970"
     CDS             complement(18212..19189)
                     /locus_tag="AC1LW8_000970"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIP4772824.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(19193..20305)
                     /gene="pdhA"
                     /locus_tag="AC1LW8_000971"
     CDS             complement(19193..20305)
                     /gene="pdhA"
                     /locus_tag="AC1LW8_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIP4772825.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(20476..21102)
                     /locus_tag="AC1LW8_000972"
     CDS             complement(20476..21102)
                     /locus_tag="AC1LW8_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HIP4772826.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            21467..22018
                     /gene="def"
                     /locus_tag="AC1LW8_000973"
     CDS             21467..22018
                     /gene="def"
                     /locus_tag="AC1LW8_000973"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP4772827.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT         7150 a         4024 c         3148 g         7856 t
ORIGIN      
        1 ttcaaaattt aacattcttg agacaattaa aacctccgct tcagaaataa taatttcaaa
       61 aatgactatg caacaacagg tagttcacgt ttttgttgtg aaacattttc gatttctaca
      121 actctaaaaa attaaaaata aaattgcaaa acatcaacat ttattatcaa tagcgataac
      181 tttatcttat catcatgatt ctaatttcgc caccacattt agtaattttt agtcataaaa
      241 tttagttata attatacgtt gttttgttta taaaatttga taatcatgag taatctcgta
      301 atatcaaaac aaaaaggaag ttaagcgttg tttggttgcc taacttccgt tattgaactc
      361 atcagtccaa tttgacaaag agtatataaa tagccaatgt ttagcattgc ttaatgacag
      421 aagtcatctc gtactaaata ttggctagta tttttttaat taaattgtct tcttatatca
      481 actttttgtt gttgtttctt tcgttgctgg tctactttga tttgtttacc tacaatcaga
      541 agtaatccca tagcaatact taaactaatc attgatgatc caccaaagct gataaatggc
      601 aatggcacac cagttaatgg aattgttgcc gaaataccgc caatgtttac aaacgtttga
      661 cttccaaagt atgtggcaat cccaacacac acaagtttat aaaaatatga tgatgtttta
      721 tttgcaaact ggaaggcacg atatacaata aagaactcta aagtaataac tagcaatcct
      781 ccgattaaac ctaattcttc gcaaataatt gcaaaaataa aatctgtatg tggttctggt
      841 aaatagccca atttcattgc actatttcct aatccttttc caaatacgcc accgttacct
      901 atcgcaagca atgaattgga aatatggtat ccagttcctg attcgaattg gaatggatct
      961 gttagcgtac taaatctggc agttaaataa cttggtaacc aaccagccat taatgcaatg
     1021 acaaatacta ctaagaatcc tagtactgct ggtataccaa atcttaggac tttgtttacc
     1081 ccaattcctg aataaaaaat gatcgcaact aaaataatta atattagtaa tgtttgccct
     1141 acgtcttttt gtaggaaaac taaaaacgta caacctaatg ctaatacaat aggacttaaa
     1201 attaattttg gtttacttaa tactctaggc atttttttac tgatcataaa tgggatatat
     1261 aatatgattg caatttttaa taactcagat gcctgtaagt tcataaatcc taagtttatc
     1321 caacttttag aaccattaat atctttacct attactaacg tcagtaataa tagtgagacg
     1381 atagttataa tcataccttt ttgcacttta atattactca gtaatttaac attcattaaa
     1441 aatgcaataa aaaatacaat tataaaactc attatgacat atgctaattg tcggttgtaa
     1501 aaatacgttc ctggaacatc gataccacca gtcaatgtgc ctttagttgc tggaaccata
     1561 cttgcactat ataccatcac taatccaatt aaactcaata caatatatgt aactaataac
     1621 ggataatcaa taaactttga ggttttacca atataccgta aaatacttct aaaattcttc
     1681 atatattcag tcatccaatt ctacaattta tacttcaaat gatttcgttt tatactttaa
     1741 ttttaaaaat ccatgttact tattatatac atgacacagt cataatcaca aatattaaca
     1801 ggtctcttga ggtaatttat agaatatttg tttacttaat atcgtgaatt gctaaaaccc
     1861 aataaaagtg actgacatgt catcaagaag acaacataac agccacttta atcagtaatt
     1921 aaactataaa tataaaatcg gcgcagctca attgtcgata tctcacgcca ttttaatatg
     1981 cttatatatt aaaccttaaa caagtgtaaa agcttcatgt aattttgaaa gttctttctc
     2041 aagacgtaac ataatttgtt tgccatcttc gcggtcaact aaacctaatt taacagcaaa
     2101 atcaacttct ttttgaagtc caaacatttg tgtatctagt acttcttcat ataatgggca
     2161 tgaaggtaat gttaaattat ctagttgaac tttaatcaga tgcaagattt catcagcatc
     2221 tttagtcaat tgtttcaaag ctgcattttt cattgttgct tgtttcgcca tgacacgctc
     2281 cccctctata taaattctca agtcttacta aaagtttatc ttactaaata ttaaaaagca
     2341 agtagattta aaatgtgtcg ttagcaattt gtgttaaaat aatgctatat acattgatag
     2401 ggagtgggaa aatgatacaa attaaaggag ccgtaaagtt tcctataaca ttagatagta
     2461 cgacttggat tttcgatgat agaaaagtta gcatcgaaga tttagaaaaa ggtgtatttg
     2521 atggttcaaa accaatcgac tttgaagata atcatgaatg gaatcgtgct atcctagaag
     2581 gacaaactaa tccgcctact ttaaattcag aaataaaata taaaaaacgt gccatgttag
     2641 aaggttcatt tgtcattaat atgacacctt ttttcaaaca ttccgaacca catgactctg
     2701 caactactat tcgattatca aatgagcatg atcatttaga tataccgata gatctattac
     2761 cctatttatt tttccaattt gcaaaagacg gtaaacgact ttacagcgat aatggtgtag
     2821 atagttttat ttacaatcaa gaagatggtt attcatatcg aatcaaccat gtaactcata
     2881 ttgaggtgat ttaatttgag acaagtgcaa tgcattatct gtgatgcaaa agtatttata
     2941 gatgagcgca caactgagtc taaacgactt aaaaacaatc ctattcgaac atttatgtgt
     3001 gatgattgta aaagtcgatt agacacacct aaacaacgtg cgcaacacta tccgctcgat
     3061 taatatctga ataaacaagt tagaaatttg aaaatgtgaa agtaacagga aataatagca
     3121 tttaaacact ttttcaaata ttatatgcca aaagagaagc taagcaactt tcatcgctca
     3181 gcttctcttt tattatgtta aattattcgt tttcttgcat catttgtttg atacgctttg
     3241 cttctttttc acgaacattt ttgtttaaaa ttttctttct taaacgtatg ctttctggtg
     3301 taacctcaac aagttcatca tcattaatga attgtaacgc ttcttccaat gttagaatac
     3361 gcggtctatt cattgtttgt gtttggtctt tcgttgcaga acgtacgtta gtttgatgtt
     3421 ttgttttagt gatgttaaca gttaaatcat tttcacgatt atgttcacca acaatcatac
     3481 cttcataaac ttcagtacca ggttccatga agtttacacc tctatcttca agtcccaaaa
     3541 tggcataagt acttgcagaa ccttgatcca ttgaaattaa tgcaccatta cgacgaccgc
     3601 caatttgtgc tttaatacgt ggtctaaatt cttcaaatgt atggttaata ataccgtaac
     3661 ctcttgtcat tgacataaat tcagtcgtat aaccaatcat accacgagcc ggtacattaa
     3721 agattaaacg tgtaagtcca ttatcagttg tagtcatatc aaccatttca cctttacgtg
     3781 cacctaatga ttcaataaca gcacctgcat tttcttgtgg cacttcacat tgcacacgtt
     3841 caaatggttc acacattaca ccatctattt ctttaataat tacttgtggt tttgaaactt
     3901 gtaattcata accttcacga cgcatatttt caataaggat tgataaatgc aattcaccgc
     3961 gaccagcaac tacccatgta tctggagaat ctgtgttaga aactttcaaa gatacatctg
     4021 tttctaattg ttgatttaaa cgttcttgaa tttgacgcgc tgttacaaag tcaccttcac
     4081 ggccagcaaa tggagaattg ttaactttaa atgtcatttc aagagtaggc tcatcaatac
     4141 gtagaactgg caatgcttct tgatggtcat gtggtgttac agtttcacca acattaatgt
     4201 cttccatacc tgaaacagca attaaatctc cagcttgtgc ttcttcaatt tctaaacgtt
     4261 ttaatccaaa gtaaccaaag attttagtta cacggaagtt tttcactgta ccgtctaatt
     4321 taattagtga tacattatct ccgacacgca ttttacctct gaatacacga ccaataccaa
     4381 tacgtccaac ataatcattg tagtccaaca atgctacttg gaattgtaat ggctcatcac
     4441 tgttatcaat tggagctggt acataatcaa taattgtttc atataatgat tgtaaattat
     4501 catcttgttt ttcaggatct aagctagctg ttccatttac tgctgaagca taaacaacag
     4561 ggaattctaa ttgttcatcg tttgcttcta attcaataaa taaatctaaa acttcatcta
     4621 caacaccctc tggacgtgct gatggtttat caattttatt aacaacaaca acaggtttca
     4681 ggttttgttc tagcgctttt ttaagtacaa aacgtgtttg aggcattgta ccttcatacg
     4741 catctactac taagacaacc ccatcaacca ttttcataat acgttctact tctccaccaa
     4801 agtctgcatg tcctggtgta tccaaaatat taatacgtgt acctttataa tcaacagccg
     4861 tatttttggc tagaatcgta attccacgct ctctttcgat atcgttagag tccattgcac
     4921 gttcatcgac atgttcattt tctctgaata taccagattg ttttaacaac tcatctacta
     4981 aagttgtttt accatggtca acgtgagcaa taattgctat attgcggaca tcttctcttt
     5041 tattagtcat ataaatagtt tccttctttc ttaagtaaaa tcccactttt aattacaact
     5101 tttttattat atcatataat tgagaaaaga taagcaaaag gtggggatag gatgaaacaa
     5161 aaaaaatcta aaaatatctt ttgggtattt tctatattag ctgttgtttt cttagtatta
     5221 tttagttttg ctgttggtgc atcaaatgta ccaatgatga ttttaacatt tatattactc
     5281 gttgcaacct ttggaattgg atttactaca aagaaaaaat atcgagaaaa cgattggcta
     5341 taagttatat cgacaataac acgcgttgat ttaagaccac acatgttcat gatgaacatt
     5401 taactggttt tatattggcg cgtgttttta ttatttaagg tataaaagcg taatatgtta
     5461 cctaattgtt aggactacat attacgcttt tggtaattat tttgattttc ttttaaatcg
     5521 ttgttcatgt aattgtatta acgcatcatg gtggggctct aaataatcat tgctaatttc
     5581 ttgatggaga ccacgatttc caactaagat tgaatttgga ccactaattg ttaatggttc
     5641 tcctagtaaa ttggaagctt gtccatttac ttcatataaa ataaccaatc cgccagcaaa
     5701 atcccatggt tgaagtcttg gcgtcatata tgcttctaaa ttacctgtag caactgaaac
     5761 gatttcaagc gctgcactac catatgccct tgcacttcta gaatcattaa caatttcttt
     5821 aaagatttct cctaaaattg gtttagttaa ccagttcgga ttgatcccaa taatgctttg
     5881 tcttagatta gaatcattca atggtttcaa gggttggcta ccacgatatg caccttcccc
     5941 tactttagca tgatataaga catcagccat aacatcatat acaaaacctg cataaggttt
     6001 accatcgata taaataccaa ttgaaattgc gaaattttct tgttgatgaa caaaattcaa
     6061 tgtaccgtct attgggtcaa caacccatac cgtacctttg gaagtatcga tgtcatgacc
     6121 atgcccttct tcacctaata cttgatgatt gggatatgtt tctaaaattg tatcaaaaat
     6181 aaaatcttct gttgctttat ctacatttgt aacaaggtca ttcggatttg actttgtttc
     6241 aattgttaaa ttttgctcca tcaattgttt aattctaatt cctgcttctt gaataagtcc
     6301 ttgggcaaat ccataaagtg ccataacatc atctcctaac atctttatta tacatcaaca
     6361 ttttataaaa ataacttctt atgataaaaa tgaaaatact aattttaatg agagcgtctt
     6421 aagccgcaat tgataaaaca tatgctacaa tatttttaaa cactatacag gaggtactct
     6481 atgacaaaat atacatttaa acctaaagat ttcaaagcgt tcaacgtaga aggcttagac
     6541 gcacgaatgg aagctttaaa cgaatacata cgaccacaac tccatgaatt aggagaatat
     6601 tttagtgact tctttacaag tcaaactggt gaaacatttt atcctcacgt agcaaagcat
     6661 gctagaagaa gtgtgaatcc tcctaaagat acatgggttg cttttgcaac aaacaaaaga
     6721 ggctataaaa tgttacctca tttccaaatt ggtatgtttg aagatcaact gtttgttatg
     6781 tttggaatca tgcatgaagc aaaagataaa gcaacacgtg caaaagtttt tgaaagaaac
     6841 tttaaagcta ttcaacaatt acctgatgat tatcgtgttt gcttagatca tatgaaacct
     6901 gataaaccat ttattaaaga tttaacggat gatgatttaa aagaagcgat acaaagagcc
     6961 atcaatgtga aaaaaggtga attctttata gcgcgtgcaa tcacaccaca agataaaaga
     7021 ttaaaaagtg acaaagcatt tattgcattt ttagaagaaa ccttcgatca gttcttacca
     7081 ttttattctg cataaataac tttgtttaaa taatagagca cttaatcaca tccatgattt
     7141 cgtgctcttt tttcttaata ttaaatcgaa cgttcaacat aataattcat acttttaaaa
     7201 aaattaaaat aaatttaggt tgacctaagc attttattag gttattatat tgtccataag
     7261 aagtagaggt gagtcaaaat gaataataaa cgacattcaa caaatgaaca attaagttta
     7321 gacgaaataa acaatacaat taaattcgat catcgcagtt caaataaaca gaaattttta
     7381 tcatttcttg ggcctgggtt attagtcgct gttggttaca tggatcccgg aaactggata
     7441 acatcaatgc aaggtggcgc gcaatatggc tatactttgc tattcgtaat tcttatttca
     7501 agtttatcag caatgttact tcaaagcatg acagtgagat taggaatagc aacaggtatg
     7561 gacttagctc aaatgacacg acattattta tcaagaccta ttgctataat cttttggatc
     7621 attgcagaac tagcaattat cgctacagat attgctgagg ttattggtag tgctattgct
     7681 cttaatctcc tatttaacat acctttaatc gtcggtgcac taataactgt acttgatgta
     7741 tttttactac tatttataat gaaatatggt tttagaaaaa ttgaagctat tgttggtaca
     7801 ttaattttca cagtgttatt catctttata tttgaagtct atatttcatc accacagttg
     7861 aatgctgtgt taaatggatt tataccacat agtgaaatca ttacaaataa cggcattctc
     7921 tatattgcat taggtattat tggcgctaca attatgcctc ataatttgta cttacattca
     7981 tcaattgtac aatctagaac atactcaaga cataacaatg aagaaaaggc gcaagcgatt
     8041 aaatttgcta cgatagattc gaacattcag ttatcaatcg catttgtagt caattgctta
     8101 ttattagtgt taggtgcatc actatttttc aactcaaacg ctgacgattt aggtggtttc
     8161 tatgatttat atcacgcctt aaaaactgaa cctgtactag gtgcaacaat gggtgcaatc
     8221 atgagtacat tatttgcagt tgcattatta gcgtcaggtc aaaattcaac gattactggt
     8281 actttagcag gacaaattgt aatggaagga tttttaagat tacacatacc aaattggtta
     8341 agacgtttaa ttacacgttc ccttgctgtc attcctgtta tcgtatgctt aatcattttt
     8401 aaaggtaatg cagctaaaat tgagcaacta cttgtattct ctcaagtatt tttaagtatt
     8461 gctctgccat tctgtttaat accattacaa ttggctacta gtaataaaga cttgatggga
     8521 cctttctata ataaaacatg ggttaatatc atttcatgga cacttattat tattctaagt
     8581 attttaaatg tctatttaat cgttcaaaca ttccaagaac tacaaagtta aaaataaaac
     8641 cattaaaatg ctggttgtat ctaataatta acaacttaac attttaatgg tttttctttt
     8701 gctatgaaca tattaatgat aaatatactc atcgtcctta tgtgtagttt cttttgattc
     8761 ttttttagaa atcaacataa tcatcacgat aacccataag acaattgcaa ttaaattaag
     8821 agccatcaaa cttacaatta tcgcgataac aaaaagtata atcgataatt ttctacgtcc
     8881 tttaatacaa attgtcgcaa atataccata aagtgttgaa attaataaaa taattgctaa
     8941 tgcacccatt gttgtctgaa gcatatctaa agaaaccttg ccaccgttct gagataattg
     9001 cttcgctaat tcttcacgtt gtgatgcatt accacttttt aaagaaaaat aggatagccc
     9061 cataactaat aaataaataa tactaatact atttgcaatc caagccatga ttaattcagg
     9121 ttttctattc atcttaaaat cctttctttg tttaatattt taataagtat actacgagcc
     9181 tatagatata cgcacattaa attacttgta tattaacata tttttcatct aatttttata
     9241 ataaacatct gaattcagat tgatgataat ttaggtgtat agtaatgaga aatcgtcatt
     9301 taaaattatg acacctaaac actttaatta aacgttatcg ataattaacc tcatataaaa
     9361 acccagatta ataagcaaaa gacctctgct tactaatctg ggtatcatca atatttattc
     9421 ttgctctttt ttgtccttaa cttcacttga attgcgttct tcagaaatat cattgttgta
     9481 aatttgacgt cgctcttcaa aattcattcg acgttgacta actgcgctag gttgattttt
     9541 cctacgttct ttcattcgtt ggttataagc tttacgtttt tcttttttct tagctttaat
     9601 ttcagctttt tctcgctttt gttgtgcttt caattctttt ggatcaactt caggttctgc
     9661 ataataacca tcatcttcaa tacctagttc ttcagtactt ttttgtttgt atttagcctg
     9721 tctggataat actgatggtt cttcctcagt gcgctcatca tgattatatt cactttcaac
     9781 tgcacgttta ggaaattgat catatttatc tctttcctgt tcttgatcat atacagtatt
     9841 tgatttatcc aattcttttt catatttagg cacatcataa taatcatctt tcggttgggg
     9901 gcgctcatac acaggttcac gataatcata aatcggtccg ttatactctt cataatattg
     9961 tggcgcaacc atttcaactt tatcttttct tataaatagc atcatcgcaa taataaagaa
    10021 taatatcgga atgattaaag taacgataag taaaactaaa ggaatcgtta caattgctga
    10081 tattaagaat aagaaacctg acaagattct tatattcata gatatgagag ctaaaaatga
    10141 aatcaacaaa caaacaataa agtaaacgat aatagcccat ataccatttt gtaaccatat
    10201 tacaaattgt gttgtattat agccatttcc agctaataat tgctggataa atgcattatt
    10261 gtttaatgta ttttcaagat tagcaatcga tgtgttatta ctgaatgaaa caagtgctat
    10321 aaacatcgta atgacagtaa gcactaataa catcacccaa cataaccaac ctagaacttt
    10381 ttcagttaat ctacttactg gacgtttaat ttgagtaaat tgttctccag tcattcgtta
    10441 caactcctta tagtacttat cccgttatta taactaaata tacagtaaat aactactatt
    10501 tatgatttta ttttaatgac attttgaaat tcaaaaagtt ttcattgtat tcacttaaaa
    10561 cttcaggtcc taaatcttta taaacttcaa ggcgttcttg ctctttctta gtcggataaa
    10621 aacgatggtc gtctttaatc tctttaggca acaattgtcg agcagccttg tttggcgttg
    10681 catagcctac gaattctgta ttttgcttgt tatttttagc atctaataaa aaattcataa
    10741 atttatatgc accctcttta ttttgtgccg tttttggaat taccatattg tcgaaccata
    10801 aattcgatcc ttctttagga ataacataat tatatttatc cccttcttgc actagaggtg
    10861 ctgcaacacc actccaaaca accgctatgt taccttcatt ttgttgaagc atcatggtaa
    10921 tttcatcacc tacgacacct cttacttgtg gtgctagttt ggttaaatct cgctctgctt
    10981 cttttaaatg gtgcgaatta cggtcattaa gattataccc aagtttattc aaactcatgc
    11041 ctataatctc tctagcaccg tcaactagta aaatttggtt tttaaattta ggattataca
    11101 atgacttcca actatcaaat gattcatttg gatacttttc tttattatat aaaataccta
    11161 cagttccaaa gaaataaggt aaagaatatt tattgcctct atcaaatgac atattcatat
    11221 aatctgaatc taaattttta atattaggta ccttattatg atctattggt aacaataaat
    11281 gatctctttt caatttttga actgtatatt cactaggaaa agcaacatca taatgtgtac
    11341 cgccattgcg aattttggct tccatcgctt catttgaatc gaaagtttca taaacgactt
    11401 gaatgccagt ttctttttca aatttcttaa ttaactctgg atcaatatat tcgccccaat
    11461 tgtatacgta aattttttga tttgtatgca cttgttcttt agatttaaac caatgactta
    11521 aagtaagaca aagcataccc acaactaatg cacctataat gagttgtaaa aaacgtttca
    11581 ttattttaca cctcgcttga ttagtttttt cttatttatc acgtattgaa tcaaataata
    11641 tcctagtatt cctaatacaa taacagcaaa cagtaatgtt gaaatcgcat taatttccat
    11701 actaattcct tttctcgcca tagcataaac ttcaactgat aacacactaa agccattacc
    11761 agtaacgaag aaacttactg tgaaatcgtc tagtgaataa gttaaagcca taaagaatcc
    11821 tcctataata gaaggtaaaa tattaggaat aataatgttg cttaataatt gtggttcagt
    11881 cgctcctaaa tctcttgcag catttaacat attattattc atttcataca gttgtggtaa
    11941 gacgataatc acaactatag gtatgcaaaa tgcaatatga gatattagaa ctgtccaaaa
    12001 tcctaaacca agaccagtaa aatggccaat cgttgtaaac ataattaaga atgatgcacc
    12061 tatgacaacg tcggatgata ccatcaagac attattcaat gttagtaaag ttactttaaa
    12121 ctttttattt cttaaataat aaatagcaat ggcaccaaat gtaccaataa ctgtagaaat
    12181 tgaggctgct aaaagtgcta cagctatcgt attaaaaatg accgacatta aatgatcatt
    12241 ttgaaataat gattgataat gctctaatgt aaaatgttca aagtgaatca tattaccagc
    12301 cgaattgaat gaatagaaca ttaaaaagaa tattgggatg tataaaatcg ctaaaagtat
    12361 cccgatatac agctttccat accatttcat atgattcacc ctctcccatt agatgatttt
    12421 gtaatgatta aaatgaatgc cataaataca attaagaata tagctatagt tgatcccata
    12481 ccataatttt gaattgttaa aaattgttcc tctattgccg tacctatatt tatgacttta
    12541 ttacctgcaa ttaatcttgt aatcataaat aatgaaagtg atggaataaa ggttacttga
    12601 atcccagtca taacaccttc ttttgttaac ggcatgatta cttttctaaa agtatagaaa
    12661 ggactggcac ctaaatcact tgaggcctgc aataaattat taggaattgc tttcatgcta
    12721 ttaaatatag gtaaaatcat aaatggtata taaatgtaac ttgccactac taaaaacgca
    12781 ccagttgtaa ataacaaatt gaatgatggt aaattaaata agtggaaaaa ttgattaatc
    12841 acgccatcat gacttaataa acctataaaa gcatatgtct ttaacaataa atttatccat
    12901 gttggaataa tcattatcat taataagata ttttgaaatt tcgaacgagt aatataatag
    12961 gcagctggat aactgatagt caaggtaata attgttatta aagcggcata taaaattgaa
    13021 tatgcaaaca ttttcaaata ttttgtagta aaaatttgtt gataattcgt gaaactaaaa
    13081 tgtccattga tatctaaaaa tgaaaaataa atgagtaata caactggtac aataataaat
    13141 ataaccatcc atagtaaata cgggattaag agaaatttat tagtattacg cattgtctac
    13201 ttcctcatag ctttcaatac gtttatcaaa ttcttcttct gtttctccag gaaccataat
    13261 atgaatggct tcaggatcaa aataaagacc aacttcactg cctacttcag cttttttagt
    13321 cgtttgtatt acccattcat aacctttatt gtctatacaa catatttcat agtggacccc
    13381 tctaaataac atagaatcaa cagttgcttt aaataatcct tcttcagctt tgattaatga
    13441 tatatcttct ggtcgaataa cgacttctac ttttttattt tcaggaatac ccatatcgac
    13501 acattcgaaa tcttgcccat aaatattcac gacataatct ctaaccatgc gcccttcaac
    13561 aatattagat tctccaataa aatcagctac aaatcgattc actggttcgt catatatatc
    13621 tgttggtgtg ccaaattgtt gaattttccc atcttttaat acaaaaagaa agtcacttaa
    13681 tgctaacgct tcttcttgat catgtgttac aaatataaat gtaataccta atctagattg
    13741 caattctcgt aattcatatt gcatttcagt acgcaatttc aaatctaatg cggataaaga
    13801 ttcatctaac aataatattt ctggttcatt tacgatagca cgtgcaattg caacacgttg
    13861 cttttgtccg ccactcattt cattaatatt tcttttttca taacctgaaa gttttactaa
    13921 ttttaatgcc tcagttactt tttgatcaat ttcggttttt gataattttt ttaatttcaa
    13981 accaaaagcg atattatcat agacgtttaa gtgtggaaat aatgcataat cttgaaagac
    14041 tgtattcact ttacgtttat ttggtggtaa attaccaatt ggtttgtttt gataaatcac
    14101 ttcaccactg tcaggatatt caaaccctgc aattaatttt aaaattgttg ttttaccaca
    14161 acctgaagga cctaataatg tataaaagta tcctgattca atatcaatat ctatgtcatc
    14221 taagatatta agatcatcat agcttttact aactgatttt aatgataata acggttccaa
    14281 ataagatccc ccctataaat atgaagctgt cgctacaata agtattcgtg tttcattgtt
    14341 tgattcgttt gacaaacgat gattatctcg tgctttaaaa tacaaaacgt cttcttcttg
    14401 tgcttgatat atctctttgc ctaaattaag tgttatctga cctgacatac aataaataaa
    14461 cgtatcagac tctgatggat taaaattttt atatgatgct ccaggcttta aagttaatat
    14521 taatggctcc atatcatatt catttgactt tgaaactaac caatttaata tataaccttc
    14581 atcatactca tcataaataa cttgttcttc cttcttgtat aatacttttt cattttcact
    14641 gtctttaaaa aattcacttg gcgtcgttcc taacacctct ataatattta agaaagtttc
    14701 catacttggt gaggcatgtt cactttctat ttgtgaaatg tagcctttcg ataagtctgt
    14761 acgttcagca agttcttctt gcgttaaatt tttaattctt ctaagatttt taattttatt
    14821 acctatgttc atttattttt ccacctaaaa aagctatatt aagtttactt ttatcaaaca
    14881 ttttgtttaa cacttgataa aggtatcaaa atatagcttt attagcaata gttttaaaga
    14941 aaaattccta ttaaattgta ttaatttcga acgaattatt ttttagctgt tactctttgt
    15001 tcttcagagt gagtttttac atcttgaact gctttgtaac tattatagcc actacttttt
    15061 tcgaaagtat taaaaatttg tttttcctct gctttagcag gcacaatttc tttaaatctt
    15121 ttatatgcac ccataaagtc gcctgccgtc acgccggatt cataatactt ctctacatga
    15181 ttaaagaaat ttatcactga aatcatctct tcattactcc agtctaaatc aattggatac
    15241 tcatattcca ttgttgaacc ctccatttca attcttttgt tagcatcgct agtataacat
    15301 gcatgtcaca tgttaacgat atctcaaatc aatttcctta cattacttta cctaaaacaa
    15361 aaaagccgta gcatatgcta cagcttttaa tgactgaatc tttatagata atcagttatt
    15421 acattgtatg gattgggtat ccgatagctt tttctgctgc ttccatagtc atctcaccta
    15481 atgttggatg tgcatggatt gttaatgcga tatcttcagc attcatacca gcttcaattg
    15541 ctaaacctaa ttcagagata atatctgatg caccagtacc aactacttga gcaccgatta
    15601 aagtatcatc ttctttaagt gtaataagtt taacaaatcc gttagtatca tctaatgata
    15661 atgcacgacc atttgctgca tatgggaatt tagaagcttt aattgctaaa ccttcttctt
    15721 tagcttgcgc ttctgaataa ccaactgtag ctaattctgg ttcagtaaag catactgctg
    15781 gcataccaat gtaatcaact tcagcagctt gaccatcaat tgcttcagca gcaactttag
    15841 cttcatagct agctttgtga gcaagtggta aacctggaac gatatcacca attgcataga
    15901 tattgctgat agacgtacgg ctttgtttat caacttctaa taatccacgg tcagcgaatt
    15961 taacacctaa ttcttctagg cctaattcgt ctgtgtttgg acgacgacct acagttacta
    16021 atacataatc agcttcgatt gttttctctt cgcctttagc ttcataagta actttaactc
    16081 cgttatctgt ttcttcagct gatttagcca tagcttcagt aacgatttca acaccttttt
    16141 ctttcatacc ttttttaaca ggttgtgtca tttgtttttc gaagccacct aagatatctt
    16201 tagcaccttc aaggatggtt acttctgaac caaagttagc aaatgctgta cctaattctg
    16261 atccaatgta tcctccacca actacaacta atttacctgg tacttcttgt aagtttaaag
    16321 cacctgttga gtcgataaca cgtttaccga atttgaaatt aggaatttca attggtcttg
    16381 aacctgttgc aatgattgca tttttaaagt tgtatgtttg tgcgctcttt tcgtccataa
    16441 cacgtaagct attgttatct acgaaatatg cttcaccttt aacgatgtta actttgttac
    16501 ctttaagtaa gccttcaaca ccaccagtta atttattaac aactgatgat ttgaattctt
    16561 gaactttttg gaagtttaaa gaaacacttt cagcaataac acctaagttt tcagaatgtt
    16621 gtgcttcaac aaaacggtga gaagcatgta gtaatgcttt tgaaggaata catcctacgt
    16681 ttaagcaaac accaccaaga ttacctttct caacgattgt tactttttgt cctaattgag
    16741 ctgcacgaat tgctgcaacg tatccaccag gacctgctcc gattactata gtatctgttt
    16801 caattgggaa atctccaact accatgtttt acccctccat taataataat tctggattat
    16861 ttaataaacg tttaatgtga ttcattgcat tttggccagt tgcaccatca atttgtctgt
    16921 ggtcaaagct taatgataat gctaatactg gtgctgcaac aatttctcca tctttaacga
    16981 taggtttttg agcaatacgg ccaattccta agattgctac ttctgggtga ttgataactg
    17041 gagtgaacca ttgtccacca gctgaaccga tattactgat tgtgcatgta gcacctttca
    17101 tttcatcggc tgttaattta ccatcacgtg ctttaacagc taattcatta atttcatctg
    17161 aaatttggaa aatagactta cgatcagcat gtttaacaac aggtactaat aatcctctat
    17221 cagtgtctgc tgcaataccg atattccagt aatgtttatg aacgatttca ccagcttctt
    17281 cattgaatga agtgttaagt gctgggtatt ttttcaatgc agaaacaagt gctttaacaa
    17341 cataaggtaa gaatgttaac ttagtacctt gttcagctgc gatttcttta aatttcttac
    17401 ggtgatccca taatgcttga acatcaattt catccattaa tgttacatga ggtgcagtat
    17461 gcttagagtt aaccattgct ttcgcaattg ctctacgcat agcagggatt ttttcagttg
    17521 tttctgggaa gtcgccttct aatgttactg ctgcaggtgc tgcaggagtt tcagcaactt
    17581 cttcacttgt agctgaagca gctgattcat ttgaagctgt tggtgcacca ccatttaagt
    17641 atgcatctac atcttctttt gtaatacgac catttttacc agatccagaa actgctttaa
    17701 tgttaacacc tttttcacgt gcgtatttac gtactgaagg cattgcttta acagttctgt
    17761 tttcatctac ttcttcagtt tgagtagcta caggtgcttg ctctgctggc gcttcctctt
    17821 tcgcaggttc ttctttagat gatgaatcat catcatgacc tttaaattgc atatcttctg
    17881 catcaggtgc atcgatttta acaataacgt caccaactac agctactgta ccttcttcta
    17941 ccataacttc ttctacagta ccagatactg gtgatgggat ttctactact gatttatcgt
    18001 tttgtacctc agctaaaaca tcgtcttctt caatagtatc tccagcttta acaaaccatt
    18061 ttacaatttc accttcgtgg ataccttccc cgatatcggg taatctaaat tcaaatgcca
    18121 cgtttttgcc ctcctaagat ttcgtaaatt aaacatttca ttttaatcaa tgagactaaa
    18181 atacgctaac ttcgttaact tttaaaatgt attaaaattc taaagtttct tttgcttttt
    18241 cgatgatgtc atttttgttt ggtaaccaaa cattttcagc ttgagtgaat ggataaattg
    18301 tatctgctgc tgcaactctt ccaataggtg cttctaatga aaggattgca cgttcactta
    18361 attcagctac aactgctgca ccaacaccag cttgacgttg tgcttcttga actacaactg
    18421 cacgaccagt tttttcaact gaagctacaa ttgtgtcaac atcgattggt tgaacagtac
    18481 gtaagtcaat tacttcaaca gaataaccat ctttttcaag ttcttctgca gctttcattg
    18541 attcttgaac cattgcaccg tatgtgatga ttgaaatgtc attaccttct tttttcacat
    18601 tagccttacc aatgtcaatt gtatattctt cttcaggtac ttcttcacgg aatgaacgat
    18661 acaatttcat atgctctaag tatacgactg ggtcattact tctaatagaa gaaattaata
    18721 aacctttcgc atcgtatggg cctgaaggaa taacaacctt tagacctgga gattgagcta
    18781 aaataccttc taagttatct gcgtgtaatt ctggtgtgtg tacgccacca ccaaatgggc
    18841 tacgaattgt tacaggtgca gttttagtac cgcctgaacg gaaacgagta cgtgcaattt
    18901 gtccagcaat cgcatcaaat acttcgaata cgaaacctaa gaattgtact tccataaccg
    18961 gacggaatcc ttcaactgca agacccatcg ctaaaccacc aatacctgat tcagctaaag
    19021 gtgtatcgaa tactctatct tcaccaaatt ctttttgtag tccttcagta acacggaaaa
    19081 caccgccgtt aacaccaacg tcttcaccaa aaattaaaac atcttggtca tttttaagtt
    19141 cagttttaag cgcatcatta atcgcttgaa ccattgtcat ttgtgccatg gcttacttcg
    19201 actccttctc tttgtaaatt tcatattgtt ctgctaagtt ttgaggcata tcttcataca
    19261 taatttccat tagagaagta acagtttgtt tttcagtgtt atcagcctct ttaattgctg
    19321 ctttaatatc agcttttgca cgttcaataa cttcattttc tttgtcttca ttccataaac
    19381 ctttgttttc aaggaattta cggaaacgta ctaatgggtc ttttttctcc cattcagcat
    19441 cttcgtctga agttctgtaa cgagttggat cgtcaccagc cattgtatga ggaccataac
    19501 gatatgtcat agtttcaatt aatgttggac cttcacctgc aactgcgcgg tcacgtgctt
    19561 ctttagttgc ttgatataca gctaacgcat ccataccatc aacttggata ccaggaatac
    19621 ctacagcaat tgctttttga gctaatgttt cagcagcagt ttgcttgctt cttggtgttg
    19681 aaattgcata gttattgttt tgaataacga aaattgcagg tgctttataa gctgctgcaa
    19741 agttaatacc ttcgtagaaa tcaccttgtg aagaaccacc gtcaccagtg taagtgattg
    19801 caactgcatt tttaccacgt tttttaagtg caaatgcaac accagcagct tgaatgtatt
    19861 gtgcaccgat aataatttgt gggcttaatg cattaacgcc ttcagggaat tgatttcctt
    19921 tgaagtgacc tcttgagaat aagaaagctt cagttaatgg taaaccatgc caaataattt
    19981 gaggaacatc tctgtatccc ggtaaaatgt aatcttcttt ttctaaagcg tattgtgacg
    20041 ctaattgtga tgcttcttga ccagcagttg gtgcatagaa acctaaacgt ccttgtctgt
    20101 ttaatgagat agaacgttga tcaaggatac gagtccatac cattctttcc attaattcca
    20161 ctaattgttc atccgtaaga tcaggtacta agtcttcatt tacgacgtta ccattctcat
    20221 ccaaaatttg aaccatttca aatttcgatt gagtatcatt taaaactttt actgcatcga
    20281 attgggcttg taacttagga gccattcaat tcaccatacc tttcccataa ttgtatagaa
    20341 attcatttta tccttaataa ttgtaccaca aataaatggg actgttaaac catttgaaaa
    20401 agttctgttt taataaaaaa agatacagat aataggaact gtattagttg tttcctacca
    20461 tctgtattat attaattatt taaattgatc aacgtcttgc ttttctttac caaccttatt
    20521 tagtactttt gtatacttat ctgatacttc ttttaacttt ttatagttct gttctattgc
    20581 ttttgatttt tcgttaacac cttgttgtgt cgcgtcattt tgatttaaat atttaaataa
    20641 tgttttctct gagtttacag cctttttata tgctttcgcg taatcactgt gtaacttata
    20701 tttttctttt aatacatcat ctaattgttt tacttcttta cgtttaacat cattatcaat
    20761 gttatcaacg tgactttttg ctttcttaaa gtcttgttca gacttcttaa ttgcgtcttc
    20821 ttctttttca aattccttta gacgatcatc ggcattttta attaaatcct ttactgcttt
    20881 accgcgaact gctgtatctt tactattgac atcttcagtt aattttttct ttttctcatc
    20941 taattcagca attttcttac caacagtttt aattggttct tcatcgtctt taattttatc
    21001 cacttgcttt aaatatgcct taatttcttt tttatccgta gtacatcctg caagcaagac
    21061 actagatgct aatactactg cgattgtttt tccaaatttc attacttgta cctccacttg
    21121 ctatttaaat taaatgataa ataaaatctg cttggaaatc aattttaaac aattacatta
    21181 cacatcttca ttactaagta tatctgttga cagttacgtc atttataata taatttttaa
    21241 agtcaagatg ataaataaat ttctaaagta tcattgcttg aaaccaacac tttaatcatt
    21301 ttttaagcaa ttagcatatc tgaaaattta catatactat ctaaaataaa cattggcagt
    21361 agttggttaa ctttgtagat atttacacat aaaaatgtat gagtatctta acaacctttt
    21421 tactgtcatt caactaaaac aaattaaaat aaggaaggtg caatatatgt taacaatgaa
    21481 agacatcatt agagatggtc atccaacttt gcgtcaaaaa gcagctgagt tagaattacc
    21541 attaactaaa gaagaaaaag aaacattaat cgccatgaga gagtttttag taaatagtca
    21601 agatgaggaa atcgcgaaac gatatggttt acgttcaggc gttggtttgg ctgcacctca
    21661 aattaatatt tctaaacgta tgattgctgt tttaatacca gatgatggca gtggcaaatc
    21721 ttatgactat atgcttgtga acccaaaaat tgtaagtcat agcgttcaag aagcttattt
    21781 accaactggt gaaggttgcc ttagtgtcga tgataatgtt gctggtctag ttcaccgtca
    21841 taatagaatt acaattaaag ccaaagacat cgaaggtaat gatatacaat tacgtctaaa
    21901 aggatatcca gcaattgttt tccaacatga aattgaccat ttaaatggtg taatgttcta
    21961 tgatcacatt gacaaagatc acccattaca accacataca gatgctgtag aagtttaaag
    22021 cacattttta ctaattatca aagcttagga taaatattat gtcctaagct ttttcttgta
    22081 caactttttc gaataaacaa cagttaaata tattcacctt tctaccaaac tttttatccc
    22141 ctcatttaaa ttttaccgtt tcatataaaa tcctttaa
//
LOCUS       DBNENW010000033        21548 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNENW010000033 DBNENW010000000
VERSION     DBNENW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.033"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            44..1228
                     /gene="rocD"
                     /locus_tag="AC1LW8_000974"
     CDS             44..1228
                     /gene="rocD"
                     /locus_tag="AC1LW8_000974"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HIP4772828.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            1267..2298
                     /gene="argC"
                     /locus_tag="AC1LW8_000975"
     CDS             1267..2298
                     /gene="argC"
                     /locus_tag="AC1LW8_000975"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIP4772829.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            2310..3551
                     /gene="argJ"
                     /locus_tag="AC1LW8_000976"
     CDS             2310..3551
                     /gene="argJ"
                     /locus_tag="AC1LW8_000976"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIP4772830.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            3567..4331
                     /gene="argB"
                     /locus_tag="AC1LW8_000977"
     CDS             3567..4331
                     /gene="argB"
                     /locus_tag="AC1LW8_000977"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIP4772831.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            4601..5080
                     /locus_tag="AC1LW8_000978"
     CDS             4601..5080
                     /locus_tag="AC1LW8_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HIP4772832.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5408..6052)
                     /gene="ausB"
                     /locus_tag="AC1LW8_000979"
     CDS             complement(5408..6052)
                     /gene="ausB"
                     /locus_tag="AC1LW8_000979"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aureusimine biosynthesis 4'-phosphopantetheinyl
                     transferase AusB"
                     /protein_id="HIP4772833.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(6065..13240)
                     /gene="ausA"
                     /locus_tag="AC1LW8_000980"
     CDS             complement(6065..13240)
                     /gene="ausA"
                     /locus_tag="AC1LW8_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aureusimine non-ribosomal peptide synthetase
                     AusA"
                     /protein_id="HIP4772834.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDLNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVVNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITHIDYMPLTTNDKVDTTRLPNPSTIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGVLEDSLSHRPIGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIARYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(13687..14937)
                     /locus_tag="AC1LW8_000981"
     CDS             complement(13687..14937)
                     /locus_tag="AC1LW8_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIP4772835.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(15323..16348)
                     /locus_tag="AC1LW8_000982"
     CDS             complement(15323..16348)
                     /locus_tag="AC1LW8_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HIP4772836.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHVVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(16521..16880)
                     /locus_tag="AC1LW8_000983"
     CDS             complement(16521..16880)
                     /locus_tag="AC1LW8_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HIP4772837.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(17096..18127)
                     /locus_tag="AC1LW8_000984"
     CDS             complement(17096..18127)
                     /locus_tag="AC1LW8_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HIP4772838.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(18140..18901)
                     /locus_tag="AC1LW8_000985"
     CDS             complement(18140..18901)
                     /locus_tag="AC1LW8_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772839.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(18898..19872)
                     /locus_tag="AC1LW8_000986"
     CDS             complement(18898..19872)
                     /locus_tag="AC1LW8_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4772840.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19886..20626)
                     /locus_tag="AC1LW8_000987"
     CDS             complement(19886..20626)
                     /locus_tag="AC1LW8_000987"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772841.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFRQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKVRNEIMETFALNHHQVEPEYYL"
     gene            complement(20968..21480)
                     /locus_tag="AC1LW8_000988"
     CDS             complement(20968..21480)
                     /locus_tag="AC1LW8_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIP4772842.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
BASE COUNT         6955 a         4146 c         3322 g         7125 t
ORIGIN      
        1 atattaattc tttaaaatgt ataaatataa aggagggaga ccgatgaatt caatcattga
       61 attaactgat tattatagct ctaataatta tgcaccactt aagcttgtca tttctaaagg
      121 taaaggtgtc aaagtttggg atactgatgg caaacaatat atagattgca tttcgggttt
      181 ttcagttgca aaccaaggcc attgtcatcc aacaattgtt aaagcgatga cagaacaagc
      241 ttcaaagttg tctatcattt cacgtgtcct ttatagtgac aatctcggga aatgggaaga
      301 aaaaatttgt catcttgcta agaaagacaa agtactcccc cttaactctg gtactgaagc
      361 tgttgaagca gccattaaaa ttgctagaaa atggggctct gaagttaaag gcattactga
      421 cggacaagtt gaaatcatcg ctatgaataa caattttcac ggtcgtacac ttggctcatt
      481 atcactatct aaccacgacg catataaagc aggatttcac cccctacttc aaggcactac
      541 aacagtagat tttggagaca ttgaacaatt aacacaagct atttcaccga atacagcagc
      601 aattattttg gaaccaattc aaggtgaagg tggcgttaat ataccaccga aaggatatat
      661 tcaagctgtg cgtcaactat gtgataaaca tcaaatatta ttgattgcag atgaaattca
      721 agttggtctt ggtagaactg ggaaatggtt tgctatggaa tgggagcaag tcgttccaga
      781 catttatatt ttaggtaagg cattgggtgg cggcttatac cctgtatctg ctgtacttgc
      841 aaataatgat gtcatgcgtg ttctaacacc aggtacacat ggttcaacat ttggtggtaa
      901 ccctttagcc attgcaatat cgacggcagc gcttgatgta cttaaagatg aacaactggt
      961 tgaacgatca gaacgcttag gttcattttt attaaaagcg ttgctacaac ttaaacatcc
     1021 tagtattaaa gaaattagag gtcgtggttt atttataggc atagagctta acacagatgc
     1081 tgcacctttt gtggatcaac tgattcaacg tggaatctta tgcaaagaca cgcatcgtac
     1141 tatcattcga ttgtctccac ctctagtcat tgataaagag gaaatccatc aaattgttgc
     1201 agcttttcaa gacgttttta aaaattaaca attaatcatt tatatatgac ataggaggga
     1261 ttcatgatga ttaaagtagg tatcgttggc ggtagcggtt atggcgcaat tgaattaatt
     1321 cgattgttac aaacacatcc tcatgtaacg attgcacaca tctactcaca ttcaaaagta
     1381 gatgaaccgt tgaaattaac atttccacat ttacaacata ttatgcaaca tttcgaagca
     1441 cttacagtgg acaataatga ctgtgatgta attttctttg cgacaccagc acccgtaagt
     1501 aaaacatgta tccctccctt agtagaaaaa ggtattcatg ttatcgattt atctggcgca
     1561 tttagaatta agaatcgtga aatatatgaa gcatattaca aagaaactgc tgcagcacaa
     1621 gatgatttga atcatgctat ttacagcatt tcagaatggc aatcgtttga taacaatgga
     1681 acgaagctca tttctaatcc tggctgtttc cctacagcaa cattattagc attacatcca
     1741 cttattagcg aaaaaatagt agatttgtca tctattatta ttgatgctaa gaccggcgtg
     1801 tcaggtgctg gtcgttcatt atcacaacgt gttcattttt cagaaatgaa tgaaaatcta
     1861 agcgcttatg caatcggaaa ccataaacac aaaccggaaa tcgagcaata tttatctatc
     1921 attgcgggtc aagatgtatc agtcatattt acaccacatc tcgtaccaat gacacgaggt
     1981 attttatcaa caatatatgt caaattatca tctgaatata cgactgaatc attacataaa
     2041 ttaatgacct cttattatgc taatcagcca tttgtcagaa ttagagatat tgggactttt
     2101 ccaaccacaa aagaagtact cggtagtaac tactgcgata tcggcatcta tgtagatgaa
     2161 acaacgcaaa cagcaatttt agtatcagtg attgataacc ttgtcaaagg cgcaagtggg
     2221 caagccattc aaaatttaaa tatattatat gattttgaag tgacgactgg cctaaatcaa
     2281 tcaccagttt atccataagg ggtgttagaa tgaaacatca agaaacgaca tcacaacaat
     2341 ataacttttc aattattaaa catggcgata tcagtacacc tcaaggcttc acggctggtg
     2401 gtatgcacat cggtttacgc gctaacaaaa aagactttgg gtggatttac tcatcgtctt
     2461 tggcaagtgc agctgccgta tatactttaa atcagtttaa agctgcacca cttattgtca
     2521 ctgaagacac tttacaaaag tctaaaggaa aattacaagc acttgttgtt aattcagcta
     2581 atgcaaattc ttgtaccggt caacaaggca tagatgatgc acgacaaaca caaacatggg
     2641 ttgctcaaca acttcaaata ccatctgagc atgttgctgt tgcttcaact ggggtcattg
     2701 gtgaatattt gcctatggat aaaattaaga ctgggaccga acatattaag gatgctaatt
     2761 ttgcaacgcc aggtgcgttt aacgaggcaa ttttaacaac tgatacctgt acaaaacata
     2821 tcgctgtatc actaaaaatc gatggtaaaa ccgttacaat tggtggtagc accaaaggtt
     2881 caggtatgat tcacccaaat atggctacca tgcttgcttt tataacaacc gatgcatcga
     2941 ttgaatcgaa tacacttcat caattattaa aatcttcgac tgaccataca tttaatatga
     3001 ttactgttga tggcgataca agtacaaatg acatggtatt agtcatggca aatcaccaag
     3061 ttgaacacca aatacttagt caagaccatc cacaatggga aacatttgtt gatgcattca
     3121 attttgtctg tacattttta gctaaagcta tagccagaga tggcgaaggc gcaacaaagt
     3181 taatcagcgt caatgtgtca ggcgctaaat caatatcaga tgcaagaaaa atcggtaaaa
     3241 ctatcgtaag ttcaaatcta gtaaaatcag ctatttttgg cgaagatgcc aattttggtc
     3301 gaatcattac agctattggc tacagcggat gtgaaattga tcctaactgc acatatgttc
     3361 aactgaacca aatacctgtc gttgataaag gtatggctgt actatttgat gagcaagcta
     3421 tgtcgaatac attaactcat gaaaatgtca caattgacgt tcagcttggt ttaggtaacg
     3481 ctgcagcgac tgcatacggt tgtgatttat cctatgatta tgtgcgtatc aacgcatcat
     3541 atcgaacata aggtggtgtt ggttagatga aatttattgt cattaaaatt ggtggcagta
     3601 cacttagtga catgcatcca tcaattatta acaacattaa gcatttacga tcaaacaaca
     3661 tctaccccat tatcgttcat ggcggtggcc catttattaa tgaagcatta tcaaaccagc
     3721 aaatcgagcc acactttgtt aatggcctaa gagtgactga taaagcaacc atgaccatta
     3781 ctaaacacac gctcattgca gacgttaaca ctgcattagt agctcaattt aaccagcacc
     3841 aatgttctgc aataggctta tgtggtttgg atgcacagct gtttgaaatt acatcttttg
     3901 atcaacaata tggatatgtc ggtgttccga ccgctttaaa taaggatgct ttacagtatt
     3961 tatgtactaa atttgtacct atcatcaatt cgattggttt caataaccat gatggagaat
     4021 tttacaatat taatgctgac acgcttgcct attttattgc atcatcatta aaagcgccta
     4081 tttatgtatt aagtaatatt gcaggtgtac tcatcaatga tgttgttata cctcaattgc
     4141 cattagtcga tattcatcaa tatattgaac atggtgatat ttatggaggt atgattccca
     4201 aagtgctaga tgccaaaaat gcgattgaaa atggctgtcc taaagttatc attgcatcag
     4261 gaaacaagcc aaatatcatt gaatctattt acaataatga ttttgttggc acaacaatcc
     4321 ttaattcata actatgaaat taaggcctaa caagttttga cacgcgagat gattccagtt
     4381 cgattatcca ttgcgctaaa acatttattt accgttcatc tcgttaacaa ttttgaatac
     4441 agtacgatac aatatgagat gtaaaaaact aataaccttt tacaaatttg tttatcaaaa
     4501 tattttaagt tttgcaaagc tttttattgt gattattttc acaaaatact ataatgagga
     4561 tagtaaatag agaggagtcc ttaagttgac gaaacgacaa atgggtatat tcatttatgc
     4621 tggaattatc ggtggcttgt tatctggaat tgtaaaatta ggttgggagg tcatgtttcc
     4681 acctcgcaca ccagaacgta atgcaacgaa cccacctcaa gagttattgc aacaattagg
     4741 atttagtagt gagtttacgc atcaaacata tacattttca aatatggaat tgccttgggt
     4801 aagctttatt gtccacttta gtttttctat cgtcattgca attatttact gcatattagt
     4861 taaaaaatac gcttacttag caatgggaca aggtgctgtt tttggtattg ctatttgggt
     4921 attattccac cttatcatta tgccaatcat gcatactgta cctgctgtgt gggatcaacc
     4981 attccaagag catctgtcag aattctttgg ccatatcgtc tggatgatga caattgaatt
     5041 agtgcgacaa cattttgtct atcgctataa attaaattaa tacactgact aacattaacg
     5101 tgagttttaa atcatcgttt gagtatgatg attgatgctc acgttatttt attaactgac
     5161 atgatatgat tccagccaac ttacgtgagc attaaagtct caaatgcgtc gtaacaaact
     5221 attattttcg gtaatttcaa tattgctcag tatattttta ccttatcact tactttaatc
     5281 tcgtcatgat tttgaatgat gccatcgtgt attcaccttt catttttcca ataaaaaaac
     5341 atctaacagt aaacatttag gcagtatagt ttaaaatcac tgcgcaatga tactgtcaga
     5401 cgtcatatta actactcaat aactgaaata cagacacttt tttatacccc cagggtgcct
     5461 gtcctaagaa acatacctgt accataaact gatcaaaaat aaattgtttg aacttcactt
     5521 cacgtgattg ataaaagtgt gattgtgtca tatcataaat gtccaatcct ttgattaaac
     5581 cttcaccaat caattttgta aaactttctt tttgtgtcca tatttgataa aaatcattta
     5641 aactacatat ttgatgtgct tcgtttgtag agaaacacgt cactaacgta cgccagtcta
     5701 aacgttgtga tatcttttcg atatcaatac caactggttc tttatcgaca acacacacga
     5761 tataaggata actatatgat aagctcacat agatgggctg tccatcacga ttgtgttgaa
     5821 caatatctgc cttacctcgt ggcgaaatgt gataatgcca ttcatgtggt aataaacctg
     5881 tgtcatgttg aattccatat tgcactaaaa tatctcccaa tctgtgcatg agtttatctt
     5941 gattgtatct atagttgact gtacgcggtt ttttatatga ccaacgactt tgtgatatta
     6001 attcttcaat acttttcaag ttactctgta attgcattac aaatactgtc ataactttcc
     6061 ctacttactt attgaatatt gttttgatat attgtgccca atgatacagc caattgttag
     6121 ttatcgttgg ccatttttca ctgatgtgat tcattatttt taatgttaat gttgtatcta
     6181 tcattgctag ttgttgttca cggtcaacac tagttaatcc aatcgtttcg tacatgtctt
     6241 gtttctgtaa aatttcatta aatgattcat cgctgacgag ttcaatttct ttgcgcttaa
     6301 cgcattctaa caaagatttc accggcattt tattaggtga tagcacatgg taaatgattt
     6361 gtggtgtgtt gacctgtgct aatgcgacaa tttgtcttgc agtcgtatcc acaaaagaaa
     6421 aatctacagg catttcagcc atgctaaccc cgatacaatc cagttgtaac aaatcattca
     6481 ttaccattga aaaacggtta gtctttatat ttctcatatg ccatcttcca ttgtaaggac
     6541 tcgtcaaatt accaacacgt acaatccgac catctaagcc attatttaca gcttctaata
     6601 cttttaattc actataaaat ttgctccgtg tatatggtga tgttagtagt tgccctttat
     6661 agacatccgc ttctgaaaat gtcacatctt ctgtgtctat atcaaaataa gttcccacac
     6721 ttatcgtaga cacatatatt aaccttgcat gatgttgttg tgccaaacgt atgacatcaa
     6781 cagtaccttg aacatttact ttttcaaatt catcatcatc accaaagtga tctgtacgag
     6841 cacctgcatg aataatcgta tccatgtttt ctggtaaaac aacatcatcc atacactcga
     6901 aatcaccaac aatgacttca atgtttgata acatcatttc aaccgtctct tctgaaaaat
     6961 aatcatttaa attcgtcatc aacttatacc ttgctatttc ctcattatca gcacgtatga
     7021 aacaataaat gcgatgactg tatccttgta gtgcttcaat cagataagca cctaaaaacc
     7081 ctgtcgcgcc agtcaatagt gtatttccta taggtcgatg acttagacta tcctctaaaa
     7141 cacccaaatt ataacgagac ataacaatct tttgtaattc cgaaagatta tccggtaatg
     7201 caactaatga ttgttgattt tggtgcatat aattaacaat ctgtcgcacg gttttatatt
     7261 ggtataatgt ctgcattgaa atatgatggc caaatcgttt taaatgcgag acaactaaca
     7321 tcgcctctaa tgagttacca ccaagttcaa agaaatcatc gtcaacaccg acatcatttt
     7381 gtttcaatac ctctccgaat acatcaacaa atgtctgctc aatttcatta gatggttcgc
     7441 tatacacttt attagactgt tgtatagttg atggatttgg caaacgcgta gtatccacct
     7501 tgtcattcgt ggttaatggc atataatcga tatgcgttat agtcttagga atcatatact
     7561 taggcagatg atcatttaaa tattgcttca aatcctgctc catttgttgc tctccgacat
     7621 aataagcatt caatatatca tgcgtatcaa agtgacttac tgtaacaaca caatcagata
     7681 taccacgaat agctaatatt gcattttcaa tttcatcaag ttcaatacgg tacccgttaa
     7741 ctttcacttg tttatctatt cttcctaaaa attcaatttg accatcagat gtataacgtg
     7801 ctaaatcacc actatgatac aactttcctt taccaaatgg attattttgc catttatcag
     7861 ccattaattc tggacggtta atatatccta tcgctaaact atcacctgca atacacaact
     7921 cgcctggcat accaataccg cataacaaac catctgacat aatatacact tggatgttag
     7981 ataagggttt gccaattgga atcgtctcag gtatcaaatc accacattga tgtgaccaat
     8041 acgatgtgat gactgttgac tcagatggtc cataggcatt gaaatacgtg ccacaatgct
     8101 tctcaatata tttaacaaag gatgccgtac tagttgcccc gcctgtaatc aacttttcaa
     8161 tataaaagtc ttccataaca ctacacatct gtaacggaat cgacgcaacc gtcacacgat
     8221 gcttattaat gagttgttgt aactgttctg gattaacacg ttcctctcta tctggaatca
     8281 caagcgtatg accatttaac aaacaacaat aaatctccat aactgatgca tcaaaaacaa
     8341 tatttgcatg ttgcaaaaat acttcattgt cgcctaattg caattcagtt gaccatgcat
     8401 gcactaaatt caacaaattt cgttgtcgta tggcaacccc tttaggcatc ccggtcgtac
     8461 cagatgtgta aatagcatac atctcattat ctaacatcgc tgtgttttca agttgattgc
     8521 catgtaaatc atcatattgt tcattttcct ttgattcaac aaagccttta gcattttcca
     8581 attgaatgac tggtaatgtc gtttcaattt caacgccgta agacatgact gcagttactt
     8641 tagcatcctc caaaattgca ccttgtcgtt tattcggaaa atcaatatcg ataggtatat
     8701 aagatgcacc tactttaact gtcgccaaca tcgccgcaat catttcaaaa ctacgttctg
     8761 taaacaaggc aacccgttga ccattgacca caccatttga taggagcatg tgcgcaatgg
     8821 catccacata gttgcgtaat gtttcatacg tcattgtcaa atcattcatg actagcgcaa
     8881 catgattacc ttgtcgtgag acaacttcat taaagtaact tatgatagat ttatttcccg
     8941 ggacattaag cattcgatcg ttaacatgcg tattgaccca atttagaagt tcctccgtgc
     9001 cgtttggtat atcacaaatt tgtagtgtat cttgatgctt caaaatataa tcaatcataa
     9061 tcatacattg attacccatg tgacgaactg tttctgagtg atataaatcg gtattatact
     9121 cgatattgat tgtatagtca tcgcgatctt cttcaatgat gaaagataaa tcaaatttcg
     9181 ccgtcactga tttgggttga atgtgtgtta atttactatg cccaaaatga gcatgattcg
     9241 tttcattgtt ttgtagtact aacatgacat caaataatgg attccgtgag gcatcatgtg
     9301 attgatctaa gtcatttact aaacattcga atgggtattc ttgatgctcg tatgcctcca
     9361 aactcatttc cttaacctct tgtaaaaact gtgtccacat tttatcaggt gacggttgcc
     9421 ctctatatac caacgtatta gcaaacatgc ctagcatttg ctccgtgcct ttatgcatac
     9481 gcgcactcat cacactaccg acaacaacat catcttttcg agcatatcta cttaacaacg
     9541 tcatgaccac actcataaag aacataaaat cagtaatttg atgcttttct acatactttt
     9601 gaagtagctg tctcatttgt tgattcattg taaatgacat cattgctcca tttgtcgttt
     9661 taatatttgg tctaacatag tctgtcggta agcttaaaat aggtacttca tctttgaatt
     9721 gagataacca atattgtcta tgtttcgtca tatcacgatg cgacatccac tcactatagt
     9781 ctttatattg caatttaagt ggtaacaata atttatgttg ataaagtgcg ttaagatcat
     9841 tcattaattg tatattactc ataccgtcat taatgatatg atgcgtatct ataaagaggt
     9901 atgcatgtaa gggacttcta atgtatctca ctctaatttg acttggcttt tccaaattaa
     9961 aaggtgctac aaattggcgc atgatttctt gttcatccgt aaaatgcgtg ttaacttctt
    10021 caaagtcaac tgcaacatct gccacaatac gttgtcgaac ctcatcatct acaacaatat
    10081 attgtgttcg taaaatctca tgtcgcgcta tcaaacgctg cactgcttgt cgcaattgag
    10141 ctacattaag ttctgatgat aaccgccata aaaaaggtac gttatacacc gtatctttat
    10201 ggtttgattt ccataataaa tacatacgct tttgtgcaga gctcagcaca taatcatctt
    10261 taactatagt ttctggaatc acttcatagt tttgttcttg aaccttagca atcgcttgtg
    10321 ctagttcaaa tacagttggc ttttgtaata aatcaccaat ttgtaatcgt ttcccagtag
    10381 atgcctctat ccgattcacc actaacgttg cttttaatga atggccacct aattcaaaga
    10441 aattatcatg aatacctact tgattcacat gtaaaatatc tgcaaaaatt tggcatagca
    10501 agtgttcggt atctgtactc ggtgctacat aggcatccgt atcgacatag tccatgatag
    10561 gcaatgcctt cttatctaat ttcccattaa tagtaatagg aatttgctca atatgcatga
    10621 aattaactgg tatcatgtac tccggtaagg tcatacgtaa ttgtgattta atcttattat
    10681 gtgataatgt atgcatcgct tcataataag caacgatata ctgatcttga tcatgatttt
    10741 gaacaataac aactgcttta ttaatacctt gtatacgctc gagcgcatgc tcaacctctg
    10801 acaactcaat cctaaaccct cgaatcttaa cttgtttgtc ctttcgatat aaataatcta
    10861 tgttgccatc gggtaacaaa cgaacgatat caccacttct atacatcagc tgatttatat
    10921 ttgaatcttt gataaattta tctgctgtca attctggctg atttaaataa cctgcagcta
    10981 acccaaagcc acttgtacat aattctccag gaataccaac gccacaccga cgctcgcctt
    11041 gcatgatata aacatgagta cccagaatcg gtttaccaat aggaatacga tttggaactt
    11101 tgttaggtat attatacgtc gttgtaaatg ttgtattttc agttggtcca taaccattaa
    11161 taatttgagg atgcttcggt ttttgattaa gcaaatccac ccacttagca ttcaatactt
    11221 ctccaccaat taataaatac tttaacggta ccaatacttc tattcgttca ctagcaatct
    11281 gattaaataa tgaggaggtt aaccacatag tattaacgtc attttcattg attaattgtt
    11341 ctaccgctat tggatttaat aattgttctt ttttagcaac aatcagcttt ccaccattga
    11401 gcaatgcacc atatatttca aatgttgcag catcaaaggc tatagttcct gataacaaaa
    11461 tcgtcgtctc ttcatttaat ggtacataat gattttgatg gaccaagcga acaatacctc
    11521 ggtgcggaat tagtgtccct ttagggttac cagttgtccc cgacgtgtaa ataacataag
    11581 catgatcttc taacgtgtta catttagaaa gattatcaat atttttccac gctatcttat
    11641 tcaaatcaat gtgattaata ttttgtttac cattttcata taaagcttgg tacgttatta
    11701 caactttagg cgttacatct tttaaaatgt actcctgacg atcacttgga tagttcagat
    11761 caattggcac gtaagcccca ccagctttca acacacctat catcgctatt atcatctcaa
    11821 tacttttttc agctatgaca gcgacacgat cattaggttc aacaccatac tggtttctca
    11881 aacggtgtgc taaatcattc gcgcgtgcat tcaatgtttg atatgttata aacactccgt
    11941 caaattgcac agcgacatga ttcggcgttg cttcaacttg ttgctcaaat aaggtaacaa
    12001 ctgtttgcgc atcatctatc tcaggcaaac ttaaattgat atcgtcatat aattgaatat
    12061 cacgttctgt catcaaatta agttcatcta cagttgttcg tttatttcca ttttcttcag
    12121 taatttgcaa ataaatattt cgaactaagt cactcagcgt ctcgattgag agcaaatcat
    12181 atgctgcact gttatatata attttgaacc cgtgttgatg tggaaaaatt tcaatatccg
    12241 ctaaagatgt atgtgcatca tgtatttgat gtacatcctc aataacatca ttacaacaag
    12301 acatcatatg atgataacaa tgaaaaatag tctctagtga aagcgaagac ttcgcgcact
    12361 gtaattgcga catattttgc aacacacatt tattaaaatc tgttgtaaaa cgttgacata
    12421 catcttttgc atcgattgtt aacgttaacg gcacaatatt tccgtgtaaa tcatttggta
    12481 aatgtgatgg tacatgtata cctaatgtga catcatgttg ttgactcatt atatgattag
    12541 ctaaatacac actaacagcc aacgatgcca tatctattga tgtcatatca tcaatcaaat
    12601 acgtttgata taaagcttgt tcaaatggat gcttaattgg aaaataacta tcaatatgga
    12661 tgtcagagtt attctctaac cgaaaatagt ttgagtctaa tgctatatgc gatgcatctt
    12721 gattgtcttt atcatcattt ctatttatat gtgcatgctg tcgagtattg ttaataacag
    12781 tattgccacg atatgcattg cataaatcat caagaaaaat atcaatttga ctatcatcga
    12841 aaatggacac atgaaaatct aatagtatat atgcagcatc agcgaactga aacaatttaa
    12901 ctttgaataa aggtgaatca ttaaaatggt aagtacttaa ttcttgctta aaaaaagctt
    12961 ctaaatcata gtttgcggaa gaagatggaa cttgttttat ctcaataaaa ggcagaaatt
    13021 catgaagtat cattgttaaa ttgtcatcgg tagtaacatc aaaaaaatgt cttatagatg
    13081 catgttgttg cacaattgtc gataatgcat acatcatttt agtagcttca acatttttag
    13141 cgagtttaac ccaatacgca ttacggtgtg tcgttgattc tgtattattt ttgtatatac
    13201 gaaaatattc ctgttgaaat ctcaaattac ccataatcat aaaaagtcct tctttcatat
    13261 cataatactc attacttact gaaattgcat gatgatatga taaccgacga aatgttaatt
    13321 aactcgttat gtaatgatta atataaaaca ccattcgcaa catatgagcg atatattcta
    13381 ccctaaaata catcttgtat catcgttaca attggtatat ttttcaatgt aaattacata
    13441 catcttcgat aaatagcaca ctacaactcg ttaatcactt tctgttgttc acatctcatt
    13501 gcaaactcaa tattgttgtt acaaaatatc catgaagcaa gtttatatta aacaaacaac
    13561 tcgcataaaa caattgttat ccttaaattt caacaaattc ttaataaatt tatctctatt
    13621 ttaattacga ccaaattaat aggttttcca tataaaaaga tgcataaaat aaatatttaa
    13681 ataaattcaa tttgtattca cttgtttttg tcccccaaat acaccagcaa caagcatgct
    13741 agcaccaatt gttaaaacga taaacatata cagtcccatt tgtaatgacg ttaagaaaac
    13801 acccaacaca atccctaacc tagctagtgt ttctgaaaaa tgaataccta atgcattaac
    13861 tgcactatat gttcctcttt tagctttagg aataatttta aagcgttgtt ctgaaactat
    13921 aggcgaataa ataatttcac ctacagtcgc aattatcata aaaacaacta ataagccaaa
    13981 ctgattaaga taggttagac cactatagcc aacaatataa atcagcaaac cagtgattaa
    14041 agctttttta aaatctattt tcaatacaac tttcgagatt gaatacgtga gtaaaatgac
    14101 gacgaccgta ttaatcatta gcaagattgc taacatctta gcacctgtaa tatcatatga
    14161 acctatactt attgtttcaa actgatcctt tagtctaata gcaatatatg aggagattga
    14221 aaattcaccc atcatgatga tactgaaccc cgaaatcaat aacatataat tacggtcttt
    14281 caaaactaat ttataactgc gaaatatatt cattatttgt aatttttgat aacgacttgc
    14341 atgcctcttg tcatcacttt gctttacttg atttcggtct tgaggtaacc aaatatataa
    14401 aataaagagt acaattaaaa atatacaagc tgctattaag aaaagtagta acatactgta
    14461 gccatacatc aagccaccta acaatgcccc aatagctacc gataagttcg tcatccaata
    14521 gctaatcttg taaatataat gttccacgtc ttcggtaatt gcatccataa ttaatgtgtc
    14581 cataactgga aattgtaatc cccaaacgat tgtaaatatg gcatatgcaa cacaaaaacc
    14641 aataatttgc cacaattgat gtgacccaaa tacgcccatg aacacaagca ttatcaccat
    14701 cgtcgcttga taaataagta ctagcaactt tttcggaaaa atctcaataa ggtaaccaga
    14761 tataatggac aatggaaatt ttagaaccac taaaccaaca agatatatac cgacaattga
    14821 ttgacttaac atatctgtta aatatagtgc tataaacggt ataaatgctg tcgtaataat
    14881 tagctgtaaa aaattgctaa tcaatcgtac tttcaaagtc gtacttaagc gtttcattaa
    14941 taactacacc cctatccaca tatatacaca ctaccattta tataaaatct attattagat
    15001 tatacgttga aacaaagaat ttttagttat acaaagttat ttaacaaaca aatgcatcga
    15061 ttttacaatg catcttgcat ataatgcctc aaaaaaatta gcactatatg actatatatt
    15121 attccaaatg aatgaaattt atcaataatt gcgtaagtga acacatatgt gcgtaaatgt
    15181 taaatcattg tcctaacata gccgtgacta tacttagatg taatcataaa aaaactactg
    15241 ccatctttac aacagcagta gtgcttatat gattcattta tataaaattg aaagcgttaa
    15301 tcgctagccc agcatcctta ttctatttag ctgtataact tttactagca atacgaccac
    15361 ttgcaacaat aatatcttta tcttggaaag gttcatgatt gaagaaacgc tctaaaatat
    15421 ctttaactcc atcttcaata cgtttttgtg cttctaaagt catacctgaa tagtgaaccg
    15481 tcatagcatt tctaggcatt gttctccatg gatgatcagc aggtgcaggt tgtggatacc
    15541 aaacatcacc agcatatcct tgtaaatgct cggatgctaa cgcttcaact aacgcatcgc
    15601 gatttacaat tttaccacgt gcagtattca ctaaataact gtgttttttc atacgactta
    15661 aaacatcttt atcaaataag ttatcagttt ctggtgttaa tggtgcatga attgtaatcg
    15721 catcacttgt tgaaacaagt tcatcaaagc ttacaaattt agacaattta tggtcttgtt
    15781 gattgattgg atcatagtgt tgtaatgtta cattaaatgg cgctaatctt tcagcaacaa
    15841 gttgtccaat tcgaccaaat ccaaaaatac caattgtttt gtgttgtaat tcatgcgcat
    15901 gattacctac ttgagacaag ttccattcac cttctactga ttgacgatga ccttcttcat
    15961 agtttctaag aagtattaat aaatccataa ccacatgttc tgccacacta actgtattac
    16021 ttcctgtaac ttcaacgaca ccaatattgt gttcacttgc tgccgctaaa tctacatggt
    16081 cagatcctac acctgctgta attgctaatt tcaagttcgg tgctttttca atacgttcac
    16141 gagtcatata tgcaggataa aatggcgcac taataatcac atccatatct ggtaaatgtt
    16201 tatctaagtc ttcaccatta tctgctaata taatgaactc atgtcctctt tcctctaaaa
    16261 atgtttttaa tcctaatgct tttttagtat taagtaattg attttcttga ccttctactg
    16321 cttctggaaa taatgctacg attttcatat ccatctctcc taaatgataa attttgactt
    16381 ccttaaagta cgtgttgcat gttgcttgtt ttaaaaatat gacaaaaaaa cggcaccgtt
    16441 tgacatacca cccccttgaa ttattgttaa attcaaagcg aagttagcaa acagcaccgt
    16501 gtaccttaca ccagcaacac ttattctatt gacttatcga aaacaaatat tgaaatgact
    16561 tcatcatcat tcaaatcaat atctgtaaat aattttacaa acttagcgcc tacaagactt
    16621 tccatctcat ttacatctat ttctttatac atctgtttaa tcttctctgt gcgtgtataa
    16681 tggagcatcg attcattgcg tttgtcgttt aggtaaaaac tctcaacttt actcaaaaca
    16741 cctgtcatat gtgcaatcgc ccaattatct ttaaacgaca ctcgaatact attgggtcct
    16801 ttacccacgt attctttacg ataagatctt actagattcg agaattgttg ttctttagta
    16861 tgcgtatcta tagtattcac cttctttaaa ataagaagtt cattaattgt tcacatttca
    16921 ttatacacct tattaacact ttttcaatga taacgattat tattattacg aaatgtatat
    16981 tttcctagtt aaaaggatat aacaaattaa cagaaaatgt ttataaacta agatttatat
    17041 tattttaaaa aatgtttcaa caaacgcatc taaacaaaaa atcagcttat cacacttacc
    17101 ccttcaactc tgcttcaagt ttacctaaat gtcttaatgt tggtgtcaat gctgcaaaga
    17161 agaatccttc ttttaacttg cgaacttgtg gcgaatatgg tgtgtacgct cttgaaccac
    17221 cattgacaac agaagcttga tttacatcta gcaataaata gccgatgtcc ttcttcaatg
    17281 atattaattc atttaaatgt gaagttaagt taccgtcatc taatattgca taatattctt
    17341 ctctaagttg acgataacgt tttttaaaag cttcaacatc atactctaaa tattgattta
    17401 ttccgttttg cgcatttgaa aatgcatcaa ttaactctaa agaactttta attgagccta
    17461 atcctattgg aatttgataa gcaataaatt gtgggcgaat agttgccgca aactgcttcg
    17521 catcatgcgt gataatttgt gattgtggca ctacgacttg attcaaagta atttgatacg
    17581 tagccgagcc atttacccct aaaaaatttg ttttttcaac gagagtgata ccatcttgat
    17641 tggcacgtag aatgaacatg acaaattcat ctgatgattc atgtttcgaa atcgcaccaa
    17701 aataatggtc ttcttgaata ttacttacag ctggcatacg tccactgaca accaattgtc
    17761 catcaacata agtgtgttca aggttcaact tttctaaatc attaaatgac ttcatcggat
    17821 tagacaatcc ggtagcacct aatatttctc cagataacaa ttgctgttgt aagtcattat
    17881 ttaaatgtgg ctgcgtggca ttttctaaat acgttgaaaa agctaattgg caccataaac
    17941 aaaatcctgt tgtcaaacaa gactgcgata cagcttcgat tacttcagca ttttttctca
    18001 atgccgcctc accgaaataa ccatctacaa ataattgttg aataaatgtt ctcggataat
    18061 acgtcccttc atcaacttct actaaatgag gatctaattg ttctttgata agcgtttcta
    18121 aagtcatcat catctctcct tattcaccaa atcttctaag tataaactgc tcaatataac
    18181 taatgaatcg atcaataata aaaccaaata atccgataaa gaatattgct gctaaaacat
    18241 cttctaagtt caacatattt cgtgcatcaa cgattaaaaa acctaatccc gattgtgcac
    18301 caatcatttc accagaaact aaaaatatcc aacttgttcc taccgccata tgtatcccag
    18361 ccatgatttg tttaaatgcc ccgggaaata atatattgcg atacaatgac cacccagtta
    18421 aatttaaatt tgccgctatt tttaaatatt gaggttcaat gtctctaacg cctttaatag
    18481 tattgaacac aattgggaaa aaagcggcga taaaaataat cgcaatcgct ggcaaactac
    18541 caataccaaa ccatagaaca acaaatggtg cccatgctat cggagatatc ggtctaatca
    18601 attgaaatag cggttcgata gcgttgtata gccaacgatt ccttccaagc aagaagccca
    18661 atggaatagc aaccaacaat gcgacaacaa agcccgctac aaatctccat aaactaattg
    18721 ctaaatgttg gaaaatttct ccagtaacaa tgaaagtcca tatacttttt cctacaagag
    18781 caggaccagg taacaataca ggttggtaat gcccaataat aatgaccatt tcccaaatgc
    18841 ctaagaaaat aataaatgtg ataataggta atataaattt gttatttgtg ggacgtgtca
    18901 tgaacgcgat gcctccttat acaatgacgg ttcaacaaag tcatcatatg caggtggatt
    18961 aaacaaatga tgttgtttta ccaatgtcgt aatttcttga tagccggatg gcttaattgt
    19021 taaatcacca taggatgtcc atgccgctga ctgtgttaaa acgtcacgac tttgtttaaa
    19081 atgatgcgtc ataatgtcta cactttgctt gcgatcattc attttaaagc cagacttttt
    19141 ataatcttgt acaaatgctt gcgctacatc cttgtgttga tcaagcaatt cccctctcag
    19201 tactagcaca cagcaatacg catcaggtat aacgtcatca ccatgtttca aagtcttacc
    19261 tttgcctaac ttttcaccca gtgcaccgaa tggttcggct acagaatacc ctgtaattct
    19321 gtgttcactc aatgcggctg gcatttctgc tggcgacatt tcatgatagc taaaatgccc
    19381 cggtttaatc tttaattgtt tacgtaattc ctcaagtaaa agataatgtg ttgaataacg
    19441 atgtggtata ccaaaatggt aatcatcgcc attattatta aattcattta agtgcatacc
    19501 tttttgtccc ataatgacat tgccttcatg atggcccaat gccacagcct ttatatttga
    19561 gcccttctgt tttgatttca tcgctagctc tattaaagtt gatgcaccat caatacgacc
    19621 actgtttaat gcgtccatta aatctggcca attattgaat ttaactaatt ctagtttata
    19681 tttcggatga ttgtattgtg ataataattt tttagtcatc atcaaattag ctgaatgtgt
    19741 aatcggcaaa tatccaattt taatcacttg ctgattttgg gcatttttag acggttcttt
    19801 agacgtcctt tgccaatcac atcctgtaat tataaagatt ccaatgatga cgattatgct
    19861 taaccttttc atcgtcactc actccttata aataatattc aggttcaact tgatgatgat
    19921 tcaatgcaaa tgtttccata atttcattac gaaccttaag taggtggcta tcattacgac
    19981 tgcgtggatg tgatgctgta atttcatatt gagaaataat attgcaccct tcacctaaca
    20041 gaacaatgcg gtcggaaaga taaatagctt catcaatgtc atgcgtcact aaaataatag
    20101 ttgattgcgt tttatgtttt agttgcacta gttgatcctg aagtttataa cgtgtaaatg
    20161 catctaatgc acctaatggc tcatccatca atataacgtt aggcttatgc acatgcgctc
    20221 gacatagtgc cacacgttgt ttcatacccc cggacagttg ctcgggaaaa tgctttcccc
    20281 tgccttctaa atcaactaat ttaagctgtg cgttaatctc ttcatcacta attttctgtc
    20341 gaaatccaat cctaatgttg tcattaatcg ttttccatgg cagcaaatta tgatgttgaa
    20401 atagcattaa acaatctgga gacggctgtt gtttaatttc gttatcaata atgacacgtc
    20461 cagatgatgg atgaataaat ccaccgatga tattgagtaa agtagacttt ccgcaaccac
    20521 tttttcctat gaaagtgact atttctcctt tgctaatgtc caaattaaag ttatgaatta
    20581 ctttatgtga tccaaagtga tgttgtaatt gttgtatttt aatcatgaca agtccccttt
    20641 taatccctac tattttaata ggaattaagt gtatatctaa agggctatgc caaacacagc
    20701 ccttatatcg tgaattgtta tatctataga tgattatggt ttggtatgag tgctcttata
    20761 acataagcgt tattcaatta acgacgttat attaatttca gctctactaa atttcaataa
    20821 aatgattgtt ttagacaata cttgcattta gttcattagt ttcgtcatat taactactta
    20881 catttatagc attgagctat tctaattaac tgcaatagaa atccacgtca aacctatata
    20941 catatatctc atcaacttgt tgtcttatta aagtttttcg atgccatcaa tcggtgtatc
    21001 aactgctttg cgcgcgcgac gtaccgcttc ttcatcaggt gcgttgtata aacaaataca
    21061 tttagacata tcttcacata catatgtgcg tttaaattca acttctggca cttcttcata
    21121 atgaacagaa tttttctttt tacgtgctaa atattgatcc atcgtaatgc cttccggaat
    21181 gttccaagtt acaaggtaat caacatgtgc atcaccattt ttaacttcat ctaaatcttt
    21241 accaactaag cgtacttctt tgactaattg aatactaata tctgcttctg ttaaaaattg
    21301 tttagcttct gcttcgtcat ttgcttccac aataaaataa ccatgagtta aattttcagt
    21361 agcttgtact tcaattaatg ttggaattgt cttcgtagat agtgatgctg cctttgcttc
    21421 tagttcttct ttcgttgatg caaaatcaag attgttagct tctaataaaa ataatgtcat
    21481 taataagacc tcctataaca atggttgaaa aaatattact ataattctta tcggattaaa
    21541 aggatatt
//
LOCUS       DBNENW010000034        21547 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNENW010000034 DBNENW010000000
VERSION     DBNENW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.034"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            54..1472
                     /locus_tag="AC1LW8_000989"
     CDS             54..1472
                     /locus_tag="AC1LW8_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HIP4772843.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLISLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGTAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1885..2793)
                     /gene="rarD"
                     /locus_tag="AC1LW8_000990"
     CDS             complement(1885..2793)
                     /gene="rarD"
                     /locus_tag="AC1LW8_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIP4772844.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDEIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFNIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3015..3590
                     /locus_tag="AC1LW8_000991"
     CDS             3015..3590
                     /locus_tag="AC1LW8_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIP4772845.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            3756..4772
                     /locus_tag="AC1LW8_000992"
     CDS             3756..4772
                     /locus_tag="AC1LW8_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HIP4772846.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGKGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            5015..5809
                     /locus_tag="AC1LW8_000993"
     CDS             5015..5809
                     /locus_tag="AC1LW8_000993"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIP4772847.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPALMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPIHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTNPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKLAVTKNNYKHFLEKYFGLDITINRIEKQ"
     gene            complement(5943..6455)
                     /locus_tag="AC1LW8_000994"
     CDS             complement(5943..6455)
                     /locus_tag="AC1LW8_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIP4772848.1"
                     /translation="MAKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6738..7496
                     /gene="vraD"
                     /locus_tag="AC1LW8_000995"
     CDS             6738..7496
                     /gene="vraD"
                     /locus_tag="AC1LW8_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HIP4772849.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7486..9366
                     /gene="vraE"
                     /locus_tag="AC1LW8_000996"
     CDS             7486..9366
                     /gene="vraE"
                     /locus_tag="AC1LW8_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HIP4772850.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQTIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9459..9650
                     /gene="vraH"
                     /locus_tag="AC1LW8_000997"
     CDS             9459..9650
                     /gene="vraH"
                     /locus_tag="AC1LW8_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HIP4772851.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            10043..10243
                     /locus_tag="AC1LW8_000998"
     CDS             10043..10243
                     /locus_tag="AC1LW8_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIP4772852.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10361..10930)
                     /locus_tag="AC1LW8_000999"
     CDS             complement(10361..10930)
                     /locus_tag="AC1LW8_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4772853.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(11190..11585)
                     /locus_tag="AC1LW8_001000"
     CDS             complement(11190..11585)
                     /locus_tag="AC1LW8_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HIP4772854.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11653..12006)
                     /locus_tag="AC1LW8_001001"
     CDS             complement(11653..12006)
                     /locus_tag="AC1LW8_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HIP4772855.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(12323..13162)
                     /gene="noc"
                     /locus_tag="AC1LW8_001002"
     CDS             complement(12323..13162)
                     /gene="noc"
                     /locus_tag="AC1LW8_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HIP4772856.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(13205..13924)
                     /gene="rsmG"
                     /locus_tag="AC1LW8_001003"
     CDS             complement(13205..13924)
                     /gene="rsmG"
                     /locus_tag="AC1LW8_001003"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIP4772857.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(13924..15801)
                     /gene="mnmG"
                     /locus_tag="AC1LW8_001004"
     CDS             complement(13924..15801)
                     /gene="mnmG"
                     /locus_tag="AC1LW8_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIP4772858.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(15868..17247)
                     /gene="mnmE"
                     /locus_tag="AC1LW8_001005"
     CDS             complement(15868..17247)
                     /gene="mnmE"
                     /locus_tag="AC1LW8_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIP4772859.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(17386..17739)
                     /gene="rnpA"
                     /locus_tag="AC1LW8_001006"
     CDS             complement(17386..17739)
                     /gene="rnpA"
                     /locus_tag="AC1LW8_001006"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIP4772860.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(17860..17997)
                     /gene="rpmH"
                     /locus_tag="AC1LW8_001007"
     CDS             complement(17860..17997)
                     /gene="rpmH"
                     /locus_tag="AC1LW8_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIP4772861.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            18728..20089
                     /gene="dnaA"
                     /locus_tag="AC1LW8_001008"
     CDS             18728..20089
                     /gene="dnaA"
                     /locus_tag="AC1LW8_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIP4772862.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            20367..21500
                     /gene="dnaN"
                     /locus_tag="AC1LW8_001009"
     CDS             20367..21500
                     /gene="dnaN"
                     /locus_tag="AC1LW8_001009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIP4772863.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         7105 a         3854 c         3266 g         7322 t
ORIGIN      
        1 ctttattctt aaattgaaat atgtataaaa catagaaaga aggacgtttt aacatggaaa
       61 acacggttaa atatcgtaag tttatactcc ctatcgttgt aggtctcctt atttgggcac
      121 ttacaccttt taaaccggat gctgtggatc caacagcatg gtatatgttc gcaatattcg
      181 tcgcgacaat cattgcttgt attacacaac cgatgccaat tggggccgtc tctataattg
      241 gatttacaat catggtactc gttggcattg ttgacatgaa aacggctgtc gctggttttg
      301 gtaataatag catttggtta attgctatgg catttttcat ttcgagagga tttgtgaaaa
      361 caggtcttgg tagacgtatc gcacttcatt tcgtcaaatt atttggtaaa aaaacattag
      421 gcttagcata ttctatcgtc ggtgtagatt taattctagc gcctgctaca ccaagtaata
      481 ccgcgcgtgc cggtggaatc atgttcccaa ttatcaaatc actttctgaa tcatttggtt
      541 cgaaaccgaa agacggatca gcacgcaaaa tgggtgcatt tcttgttttc acagaattcc
      601 aaggtaattt aattactgcg gctatgtttt taactgcaat ggccggtaac ccccttgcac
      661 aaaatttagc atctagcaca tctaatgttc acattacatg gatgaattgg ttcctagctg
      721 ctttagttcc tggacttatt tccttaattg ttgtaccttt tattatttat aaaatttatc
      781 caccaactgt taaagaaaca ccaaatgcta agagttgggc tgaaaatgaa ttagcgacta
      841 tgggtaaaat cgctttagct gaaaaattta tgattggtat ttttgtcgtt gcgttaacac
      901 tatggattgt cggaagtttc attcatattg atgcaacttt aacggccttt attgcgttag
      961 cattgttatt attgacaggc gtcttaacat ggcaagacat tttaaacgaa acaggtgctt
     1021 ggaacacatt agtatggttc tcagtattag tgttaatggc cgaccaatta aacaagcttg
     1081 gatttattcc ttggttaagt aaatccattg ctacaagtct tggtggctta agctggccta
     1141 tagtactggt cattttaata ttgttctact tctattcaca ttacttattt gcaagttcta
     1201 cagcacatat cagtgcgatg tatgcagcat tactaggcgt tgccatcgca gccggtgcac
     1261 caccattatt cagtgcatta atgttaggtt tcttcggtaa cctattagct tcaacaacac
     1321 actatagtag tggcacagcg ccgattctat tctcttcagg ttacgtgact caaaaacgtt
     1381 ggtggacaat gaacttgatc ttaggtttcg tctactttat tatctggatt ggtttaggat
     1441 cactttggat gaaagtaatt ggtatatttt aaaatattta aattagcgct cgaatctcat
     1501 tgatttgggc gctttttaat ttgtatttaa aatcaacctt tgctaaatca agactccctt
     1561 tttaaaatac gtttatcctt taaatcattg cgtgcttcac taaaaatcta tataacgctt
     1621 taaatcattg cgtaacatca cagaaaatac atttctgtat aaacgcgctt cgttgattaa
     1681 cattacgtat gccctcataa atcatgaaat taaaaacacc ttcgtttaaa ttcattttaa
     1741 gtgcgaattc aacgaaagtg atttatttca tatttaatgt ttcaaattta tacagctgtc
     1801 gctgttactg cacacatacc tcagtctcac cttatcatga cacaaaggat attgaaaaca
     1861 cgtataatta tctttcctcg tattttatag ggtttttgcg accggatgtt tcttcaattt
     1921 aatgtattga gaaagactat ataacacaat acctgtccaa ataaatataa acgtaattaa
     1981 ttgatctata ttaaaaggct ctttgaaaac aaatatgccg agtacaaaca ttattgttgg
     2041 tccaacgtat tgaataaatc ctattaacga aagtggaata cgttttgccc cggctgagaa
     2101 taggattagt ggtattgccg taatagcacc agaaaataac aaccaaaatg atgacatgtt
     2161 caatccaaat gacatctgat gttgctgcca taaataaata acgtatatta gtccagcagg
     2221 tgcggtaaca atacattcaa tcgtaatact gctgatggca tcaatatgta ctactttttt
     2281 caataatccg tatgtaccaa aggataacgc taatataata gaaacgattg ggaattctcc
     2341 aatcttgagc gtcatatata atacaccgat gaatgcgaat aaaatagcta gccattcaaa
     2401 tttattgaat ctttctttta aaaagataag tgcgagcaaa atgctaacaa gtggatttat
     2461 ataataacct aaacttgttt gtaggacgtg accgttcgtt acagcccaaa taaatgtacc
     2521 ccaatttaat gtaatgacat agcctgctac gacaatcgct aatagctgaa tgggcttgcc
     2581 taacaattga ttcatatctc gttgaaatgc attgcgttgt ttttgtccaa ccgcgagtat
     2641 gaaaatcatg aatattgctg aaaatataat acgaaaggct aaaatttcaa atgcgcctat
     2701 ttcatcaacg aactgccaat atataggtag tattccccac agaatgtatg cactgagtgc
     2761 taaaaatata ccttttttat attctgtatt cacttacata cctccttact ctcccaaatt
     2821 tttaacatgt tacgctcact tggttatgct aatataacga ttttactaat aatatttcga
     2881 taaagatatc attttgttta tatttcccac atttattcac caaccactaa acaatattaa
     2941 ttttataaat aattctgtac aaatcagggt atattgccag aaagactacc atacaacata
     3001 aaggatggat acaaatgact ttacctaaaa ttggaaagcc tgcaacacgc gcgctaaatt
     3061 cacaaggtat atacacatta gaagcagtat cacaatatac gaagtcatct ctaatggaga
     3121 tgcatggcgt tggtcctaaa gctatatcaa tattggaaca agctttattt cagcaccaac
     3181 tacattttaa aacggaagtt cagtcatcat taccttttaa gttaacagga gatgtgtcat
     3241 gcaatcatgc gcctaaacgt caacaaatga tagattttat agttgctaca gcagcattag
     3301 atattgaatt attacgttca ctggttacaa cagaatttat ttggtctgtc cctggccgct
     3361 ttgacatata cggacctcaa atattaattc aagaactatc taatcattac aatcaggttg
     3421 ctagtctcaa tatccattcc agcatcacac atgggtgctt aggttccatg catggtattg
     3481 aaatattaaa aactggtaaa gagattcatt ttgctcattt ttttgaattt gaaaatcata
     3541 aaaaggatgc caagttaagc aaagtgacat cttacattgt tgttggttaa tttatcaaat
     3601 tatatatatc gcaaaaaaat cttttgtggt tacatgtata ttgttatata acaaataatt
     3661 tggcataaca tttttgttat atgaacactt attactagag tgcccattga acatacagta
     3721 tgattaaata tcatgttatg aaagaaggat atattttgac tgtttttaaa aatgagcgct
     3781 taagctggtt accatacata gctattgtca ttttgttaca cgttattggg tttagttttt
     3841 tatggattgc tggaaaagac catcatatct tatttggtaa ggggattctt gcatatacat
     3901 taggtttgcg tcatgcattt gatgcagatc acattgctgc aatagataat acggttcgca
     3961 aattattaca gcaacgcaaa gatccatctg gtgtgggttt ctatttttca attggacatt
     4021 catctgtcgt atttttaatg gctgtttttt taggggtatc tgtaaaatgg gctaaagatg
     4081 aattaccgca tttccaagat attggtggaa cgattggtac actagtttca ggtttctttt
     4141 tagtgcttat cggtgtgttg aatctaatta ttttaatctc tttaattaac ttgttcgcta
     4201 aattacgtcg tgaacacatt gaagaagctg aagtcgatgc attacttgaa tctagaggat
     4261 tggtttctcg atttgtagga ccttatttca aattaatcac gcgtagttgg cacgtattgc
     4321 cacttggctt tttatttgga cttggttttg atacagctag tgaaattgcg ttactcgctc
     4381 tttcttcagg cgcatcacaa caagccattt cgtttatcgg aatcttatct ttaccaattt
     4441 tattcgcatc aggtatgagt ttattggata cattagatgg tgttgtaatg aagtatgcct
     4501 ataattgggc attcttcaat cctattcgca aaatctatta caatataacg attactgcga
     4561 tatcagtcat ggcagcatta gtgattggga tgattgaatt gctacaaatt cttgctgata
     4621 agctagattt acatggtgcg ttttgggcat tcattggttc gatcgaattt gattatttag
     4681 gctatatttt agttgcatta tttttaatta cttggcttat ttcaagttta atttggaagt
     4741 ttggtcgcat agaacacaaa tggtctaaat aaagtaactt gtcttgcaac tacctttgta
     4801 ttgttgcatt tttagacacc ctgcacactt cttccattat gctacgctct cagtagtcat
     4861 ctttaaatcg tttcttttag agatgacttc attttgagag cgtatttttc tatttaaaat
     4921 gtatttatcc aaaattcaat gctttcgaat ttaaaatttc agtatttctg tttaaaattt
     4981 aattataatc tttaatacag gaggcgctat gcttatgaat attgcgaagt tagagaatta
     5041 tttacaaatt gattcatctc gttataatcg accgagtatt gaagcattaa attattatgc
     5101 aacacgtttt atgttaactg taccttttga aaatattgat gttcaaaata gtaagcctat
     5161 atctataaat atcgatgcac tttttaataa aattgttcat gataaacgtg gtggtttttg
     5221 ttatgagtta aatacatttt tcaaagccta tttacaacaa aaaggattta atcctgcatt
     5281 aatgtcagct actattcaca cacccggtgg cggtcgtagt ctgaacggtt cacatgcttc
     5341 acttgttgtt tctataaatg atgtattcta tgtaactgat gttggctttg gagacttacc
     5401 tatacatgcc attcctatta cttcatctga gcatacacaa ccaattactg atatcagtgg
     5461 tacattccgc gctattttta acaacgaaga caaggatatt ttctatgttc aaaaattcga
     5521 aaatgatcat tggcatacaa aatatgaagc tgaattcaaa cctaaacaaa tagaagattt
     5581 taatagcaac atcgaatata atcaaaccaa cccagattca atatttgtac agcatttatt
     5641 aattacaatg ccacaatcat ttggccgtgc tacaatgtct gaaaatcatt taactttaac
     5701 aaggaatggt agctcagaaa agttagctgt cactaaaaat aactataaac attttttaga
     5761 aaaatatttt ggactagata taactattaa tcgtattgaa aaacaataat agtaaaatat
     5821 aaaaatatgg gcgtatctta cttattgtag gtagcgcccc gtttttatgc ttcataaaca
     5881 caacaaaaag gatatgacac aaacttcatg tcacaccctt cttttgaacc gcttcatttt
     5941 acttaagcaa gtgcatctac aacataaatg acacctacga atacaaatgc aatagcaatc
     6001 atcgtcgaaa taaagacgat acccattaat ggattaaata ataaaatgac accgaaaaca
     6061 atacctaata aattaaaaat cactgaaatt agttttaagc taccactacc cgtaaacgta
     6121 aataatcctg caatagaact aaaaataaac cagaatgcaa acatgtagat aaaaaatgcc
     6181 gagctagcgc caacattaaa aatgacaata agaccaaaga gaatatctac aatccccata
     6241 aacagaatcc aattttggtt accacctact aaagcttttg cttttctacg gtaaatgatt
     6301 tgaatcacac cgttaattaa tacaaacagt ccaatcaacc aggtaatagc ataaaaattt
     6361 tcaattggaa atgtaaaaat aacgactgcc aacattaata ataatacacc cattattaaa
     6421 ctagaccatt taataccttg gtttcctttt gccataacaa tcaattcctt tcaaataaag
     6481 ccctctttta atttttacaa aaaatttatt atacaataat taaattccct tttctcaatt
     6541 tacacaaaca tcatcactta gaaagcacca ttgcttttgt ctaatatgta cgatgaatct
     6601 gtatcctcac tcaaaaatct actacgtagc gatagctttc aactctgtaa ggttcaaaca
     6661 ttgaattgta agtttagata aaggctacta ctccaaacat tatttaaaat gaaattaaat
     6721 acaaaaggag tgagactatg acaatattat cagtgcaaca tgtttcaaaa acatacggta
     6781 aaaagcacac atttcaagca cttaaagata ttaactttga catacaaaaa ggcgaattcg
     6841 ttgcgattat ggggccttct ggatcaggta agacaacctt attaaatgta ctaagttcaa
     6901 ttgaccaaat ttctagcggt agcgtgattg ctaacggaca agagcttaat aaacttaatc
     6961 aaaaagcact tgccaaattc cgtaaagaat cattaggttt catcttccaa gattacagta
     7021 ttctgccgac attaactgtt aaagaaaaca ttatgttacc tttatctgtt caaaaaatgt
     7081 cgaaggcaac aatggaagaa aattacaaag cgatcacgac agcattaggt atttatgacc
     7141 taggaaataa ataccctagc gaattatctg gtggtcaaca acaaagaact gcagcagcga
     7201 gagcatttgt tcacaaacca caaatcatat ttgcagatga gccaacaggc gcactcgact
     7261 cgaaaagtgc aaatgactta ttacaacgtt tggaagaaat gaataaatcg tttgatacaa
     7321 ctattgtcat ggttacacat gatccggttg cagcaagttt tgcagaacgt gtcatcatgc
     7381 taaaagatgg ccaaattcat acacaacttt atcaggaagg acgttctaaa caggcctttt
     7441 atgaagacat tgtacatctt caatcagtat taggtggtgt ctcaaatgac atttaaccat
     7501 atcgttttca aaaatttacg ccaaaactta aaacattatg caatgtattt attttcatta
     7561 ttttttagca tcgtcttata tttcagtttt acaaccttac agtttactaa aggtgtaaat
     7621 aatgacgact cgatggccat cattaagaaa ggtgctttag tcggatcaat atttttattt
     7681 atcattattg tcatcttttt aatgtatgcc aatcatttat tcgtgaaacg tcgtacacgt
     7741 gaatttgcgc tatttcagtt gattggttta acacgacaaa acattttgaa aatgttagca
     7801 cttgaacaaa tgattgtatt tttaatcaca ggtgttgtcg gtgtattatg tggcattgca
     7861 ggtgcacaat tattgctttc catagtatct aaattgatgt cgttatcgat taacttatcg
     7921 atacatttcg aacctatggc ccttgtttta actattttca tgctaattat tgcgtatgta
     7981 ctgattttat ttcaaagtgc tttatttcta aaaagacgta gtatcttatc aatgatgaaa
     8041 gactcaatta aaactgatgt tacaactgcg aaagttacag ttatagaagt catttcaggc
     8101 gtattaggta ttgctatgat tgcactaggc tattatatgg cgacagaaat gtttggaaca
     8161 tttaaagcac taactatggc tatgacatca ccgtttatca ttttattttt aacggtagta
     8221 ggcgcctatt tattcttcag aagttccgtg tcactaattt ttaaaacttt gaaaaaatct
     8281 aaaaatggac gcgtatctat tacagatgtt gtatttacat cgtctattat gtaccgaatg
     8341 aagaaaaatg cgatgtcttt aactatcatt gcaattattt ctgcagttac agtaactgta
     8401 ttgtgtttcg cggcattatc taaatcaaat acagatcaaa cccttacatc tatggcacca
     8461 aatgaattta acgtggtcgc tactcaagat gctaaacaat ttgaaactaa actaagccaa
     8521 caacaaatta cattttctaa aaatgcttac gaaactatta ctgtagataa tgttaaagat
     8581 caggttatta ctttggaaaa tggtagcgat tcaggtcgca cgaattctat tttaagtgca
     8641 aataacaagt taacaggcaa caatgccatc attacaaata caaaatcgct gcctaacatt
     8701 attaatatcc atttaaacaa agatttagta gtaaaaggta ctaagaatga aactttccgt
     8761 gttacacaag aagacaaagg taaggtttat cctttaaatc taagcttcaa ctcacctgtc
     8821 atcgaagtaa gtcctgaaaa gtatcagcag ttgaaaacac aaaataacgt tcatactttt
     8881 tatggatatg atattaaaca aacatcacaa aaggaaaaag ctcaaacaat tgcaaaacag
     8941 tttggagaca aagtcatcac gtatgatgaa atgaaaaaag aggtcgatgc tactaacggt
     9001 attctaatat ttgttacatc atttttagga ttagcatttt tagtagcagc tggatgtatt
     9061 atttatatta aacaaatgga tgaaactgag gatgaactaa gtaacttcag aatattaaaa
     9121 cgcataggct ttacgcatac agatatgctt aagggattat tattaaaaat tactttcaac
     9181 ttcggtttac cattattgat tgctatatta catgcagtat tcgccgcaat agcatttatg
     9241 aaattgatgg gtaatatttc cttcatgccg gttattgtag taattgttgt atacacactc
     9301 atttacatta cttttgcttt aattgctttt gtacattcaa acaaattgat taagaaaacc
     9361 atttaacact tatgattaaa agatgcttca ttgagatttt gattttaagc aatgcagtat
     9421 aactattaac tgatttataa aaaaggagag attgaatcat gaaatttaga gaattagtaa
     9481 aacaatcata cgaggatttg aaaaatttga ctgtaacttg gtataacgtc attgctttag
     9541 caatgattgt tattgtctta agcgtagtta caacaccaat tataggtatt ccagctggtt
     9601 tattaggtgg cgcctattat ttaaaacgtc gtgaagaaaa aggcaagtaa gcacattcca
     9661 tcggtataga acattgctat gacctttcaa gtcagatttt aatatcaaca agtagcgttt
     9721 atcgctgctt gttttttatg gattgcttat aaaatcatca tgtttaaacg atatgtcact
     9781 ttgaaaacta gcaaacacca taaaagacta caattttttt atttttataa acccctttaa
     9841 atcaacgttt ctaaggtttt taaaaatctt ttttaattta ttttcaaaaa acactgtaca
     9901 ttatgccaat atgagcgtat agttggtctt acgtaataaa agctcgtgaa ttaaattgta
     9961 gtgtatttgt ttagaatatc ctctttttta gttatgaatt tgttacaaat attaagtgca
    10021 aaagcacatg gaggttttct atatgaataa cggtacagta aaatggttta acgcagaaaa
    10081 aggttttggt ttcatcgaac aagaaaatgg cggagacgta ttcgtacatt tctcaggtat
    10141 cgctagcgat ggctacaaaa ctttagaaga aggtcaaaaa gttactttcg aaatcactga
    10201 aggtcaacgt ggagaccaag cagttaacgt acaaactgtt taatcttaca aaataaaacg
    10261 actcattata aatgaatcgc tttgattacc aacaaggttc taaatcttgt tggtattttt
    10321 ttatgctatt ttggataata tgagacaata cgatgatgat ttataaaaac ttatttgcca
    10381 aatacacact aaggaaaatt atgaggctga gtataccaat tactgatata atagaaagcg
    10441 catttgtcat cgtatttcta gctttagtcg tttgtacttc actaggatct tttccattca
    10501 taaaagcaac aattcgatcg tagttgtcca ttttatgcac tttttttaaa tcttctattt
    10561 tcatagatgc ataaaaacct attcccaaaa tgattgccac cgttacaccc caagtccaat
    10621 tccaataaac aactaaaggt cccattaaaa ttgcagctaa tgtcatgaaa ataagcatca
    10681 catatgtcca taagttaaga cttcgttgcg ctttaatatc aggtacaaat ggaatggtcc
    10741 cttttactaa atcatctaaa gttacattga acaattcgca catcattaat aagttatgta
    10801 tgtctggtaa gcttttgtca ttttcccaat tagaaatact ctgcctagat acatataact
    10861 tttcagcaag atattcttgt gaataaccat ctcgttccct atatttttta atttgtttac
    10921 tcagattcaa ttacatacgc ctccagtcaa ttagtacaat cattacaatc agactatcag
    10981 ataaacttag ttaaagtcac ctttatgatt gactaaatgt tttgacatac gacaacatca
    11041 gggtttgaag acttcctcaa gacagacctg tatgattacg cgcttgtaat tttctcaatg
    11101 ccaacttgaa tttcatttat taatgatgca acttttccat gctattcaat gcttgcgaag
    11161 ttagtcctat ttaagagatt acgttcacac tatttactat tcgattttcc agctttccaa
    11221 ctatgctttt ctaatctttt ttgtgctata aattctactg cttcaaaaat acatactgca
    11281 ctgatgaacc agcttaggaa accaacaaca atgctaatca accacatgtg cgtcatatgt
    11341 aacatcatcc atgtaactaa aatacaaact aatacccctg tcataccaaa cactgcgcca
    11401 cgacttacat gcacagcgac tttatcacca tattgcatac ctgtaataaa catagacact
    11461 aaaaatactg ccggaaacgt tgcaaatata ccgccaaatt ctttccaagg tagtgttaca
    11521 gatacaatat aacttaataa tactgcaaat ccccccacaa aaaatttcat taatgttaat
    11581 ttcatgatgt tctgcctcca aaaatttatt acttaataac tgaatacaac taattatcac
    11641 gcattttcca aactataaat gtctaatact gaatattgca agggaaataa caaaccaaca
    11701 aacaactgtg aatattgcgc ctttccgata acctttatgc ttaatataga gagatgtcaa
    11761 aaatacagtt aatatacaag acagaatacc gacaattgct ccagtactaa gattcatcga
    11821 catctccact aattgtgtac cacgatgatc caaagctaat gcgataatag ccgcaagaaa
    11881 gactgccggc atagtagcta taatacctcc taacttacca cctaccttat cagcaataat
    11941 tgaagctaat gcaacagcga taccaccaat gacaaaatga agtattgcac ttccaattga
    12001 aaacatcttc aacgtacctt tctataatta attcaataca aaacgtatca ttcacacaaa
    12061 gtgtaaaact taaattttct actgtaaagg caactcaagc gatgttcata aagctttcat
    12121 gatactttca taaactagca ataaagggtt caaatgaagg tatatccagc tcaaattatt
    12181 gaaaattacc attaactgta attttcaaat caacatataa atataaattt ttatgtttca
    12241 cgtaaaacaa aaaaagacaa agctgttatg atcttagctt tgtcttttat gtgttagtca
    12301 tcatgtatac gacatcctac tactaacgtt tatatattcg aatttttatt tcataataat
    12361 cttcatgatc tttgtcttta tgctcaacat gtagtcctgt ttgttgaatc gcttggatac
    12421 ttttgcctac ctcatctcgt gcttgagtga catcttgtgc aaagcgaagg ttttgtgctt
    12481 tgactttttc gggcccagtt ttttggcgta ctctatcttc agtctgtttc acatttagtt
    12541 tttgtgcaat gacttgctca atcaacgctt cttgttcact atcagacaat gataataccg
    12601 ctctcgcatg gcgttcagta attttacctt ctcttaagcg aagtagtact ttcggcgcca
    12661 acttcaataa acgcaactta tttgcaataa agctttgact tttacctaaa ctttttgcca
    12721 attcactttg cgttgtatca ccaatttcca ataatttctt ataggcttcc gcttcttcaa
    12781 caacagacaa attttctctt tgaatattct caattaatgc aacaacagcc gtctcttcat
    12841 catccatatc acgaataata acgtctgctt gaggtaaatt tagtgattgt attgctctaa
    12901 atcggcgctc tccagcaata atttcaaaca tatcttcttc aatcggtctt acaacaatag
    12961 gctgtaataa accatgttca tgtattgatt cagcaagttc tttaatttta tttggttcaa
    13021 acacctgtct tggttgataa cggttgggaa cgatacgttc aatttgaatg gattcaacat
    13081 tactattgcg atcttcttca atatgtccaa tgatgtcatc tttgtttttc aaaccaaata
    13141 attttgaaaa aggttttttc attatccatt cgctcctttt atgttagttg taaaggatta
    13201 tgcattattt ttcaagtaaa ggagtcttat taggcgtccc tggttttctt ggatactttt
    13261 tcggcgtctg tcttttttta tcaataatga acatctggcg ctctccagca tcttctggca
    13321 attcgaaagt atgtgtttct gtaacattac cacctaacac actaattgca aattttgctt
    13381 cttctaattc ttcttcacct tttgaagatt ttaatgcaac aaactgtcca ccttttttaa
    13441 ctagcggtaa acacaattca cttaacacgg ataatctagc tactgctctt gcagtaacaa
    13501 catcataaga ctccctgtag acacccttac caaatgtttc tgctctatcg tgtataaagc
    13561 tgacatcctg taattgtaat tctgacgcta aatggtttaa aaattgaata cgcttattta
    13621 atgaatcaac aatcgtcact tttaactgcg gaaacattat ttttaacgga atacttggaa
    13681 aaccagctcc agcgcctaca tcacatatac ttataggctg attaaaatca aaataaaaac
    13741 taggtgcaat ggaatcataa aaatgtttca aatatacatc gtgttcatct gtaatacttg
    13801 tcaaattcat cttttcattc cattcaacaa gtaaacgata atatgtttga aactgttgtt
    13861 tttgagtctc agttaattga atattatgtt cttttaattg ttctgctaac cattctacag
    13921 tcattagtca ctcacccttt ggagtttacc ttgttctaag taaatcaata atatagatat
    13981 gtctgctgga tttacccctg atattctaga agcttgtgca atatttaaag gttttacttc
    14041 tgataatttt tctcgcgctt cagtcgccaa actatcaatc ttactataat ctaagtcttc
    14101 tggaattttc ttctcttcca tacgcttaac tttctcaact tgttgtagtg atttattgat
    14161 ataaccttca tattttgttt gtatttctac ttgttcttca acatctgcat tcaattgatg
    14221 ttcttcttct aaaatttcta aaattatatc gtaagtcatt tcaggtctgc gtaataaatc
    14281 gatagctaaa ataccatctt ttaagcgaga accaccatgt tgttcaataa tcgcttgcgt
    14341 atgttcgttt ggtttaatac gaatatctga taaacgctta atttccgcat caatttgctg
    14401 acgtttttca ttaaaacgtg catatcgttc ttcagaaatc ataccaagtt catatcccat
    14461 atccgtcaat ctcaaatcag cattatcatg acgtagtaac aaacgatatt ctgcacgtga
    14521 tgttagtaaa cgataaggtt cattagtacc tttagttaca agatcatcga ttaagacacc
    14581 aatatatgca tctgaacgac ttaatatctt ttcgcctgtg tttaacactt tacctgcagc
    14641 gttaatacct gccatcaatc cttgtcctgc tgcttcttca taaccagatg taccattaat
    14701 ttgacctgca gtatataagt ttttaatcat tttcgtttca agtgtaggcc ataactgcgt
    14761 tggcacaatc gcatcatatt caattgcgta gccggcacgc atcatatctg ctttttcaag
    14821 acctggtatc gtctctaaca tttgacgttg cacatgttca ggaagacttg tagacaatcc
    14881 ttgcacatat acttcatttg tattacgacc ttcaggctct aagaaaagtt gatgtcgcgg
    14941 cttatcatta aatcgaacaa atttatcttc aattgaaggg caataacgtg gcccggttcc
    15001 tttaatcatc cctgaataca ttgcagatag atgtaaatta tcatcgataa ctttgtgtgt
    15061 ttcagcatta gtatacgtta gccaacatgg caattgatct aatatatatt ctgttgtttc
    15121 aaagctgaat gcacgaccta catcgtcacc tggttgtatt tcagtcttcg aatagtcaat
    15181 tgtttttgaa tttacacgcg gtggtgtacc tgttttaaaa cgaacaatat caaaaccaag
    15241 ttctcttaaa ttgtctgata atgtgattga tggtaattgg tgatttggtc cacttgaata
    15301 cttcatatta cctaaaatga tttcaccacg taaaaatgtt cccgttgtaa taattactgc
    15361 tttagataaa tactctgtac caatatttgt acgtacacct ttaacttcat tatcttctat
    15421 aataagttcg tctaccatac cttgcattat atgcaaattt tcttcatctt caatcacgcg
    15481 tttcatttct tgttgataaa gtactttatc tgcttgcgct cttagtgctc ttacagcagg
    15541 tcctttacct gtatttaaca ttctcatttg aatgtgtgtt ttatcgattg tttttgccat
    15601 ttgtccacct aaagcatcaa tttcacgaac aacgatacct ttagctggtc cacctacaga
    15661 tgggttacat ggcataaatg caatattatc taaatttatt gttagcatta atgttttagc
    15721 accacgtctt gcagatgcta aacctgcttc tacacctgca tgtcccgcac ctataacgat
    15781 tacatcatat tcttgaacca caatataaac ctccttattt gatatcttac tagccttctt
    15841 aagacggtat tccgtctatt tcaattacta tttacctaag cagaattgac tgaataactg
    15901 atcgatgagt tcatcacttg cagtctcacc aataatttct cctaatattt cccaagttct
    15961 agttaaatca atttgtacca tatccatagg cacaccagat tctgctgcat caatcgcatc
    16021 ttgtatcgtt tgtcttgctt gttttaataa tgaaatatgt cttgaattag aaacataagt
    16081 catatcttga ttttgtactt ctccaccaaa gaacaaatct cgaatttgta tttctaattc
    16141 atcaatacct tcttgtttta acattgaagt ttgaattaat ggcgtatcac ctatcatatc
    16201 tttaacttca ttaatatcta tgttttgctc taaatccatt ttattaacaa ttacgattac
    16261 atcttcattt ttaaccactt catataatgt gtaatcttct tgagtcaatg cttcgttatt
    16321 gtttaataca aataaaatta agtctgcttg gctaagagcc tttctagagc gttcaacacc
    16381 aatcttctct actatatctt ctgtctcgcg tataccagca gtatcaacta atcttaatgg
    16441 cacgccacga acattgacgt actcttctaa gacatctctt gtagtacctg ctacctcagt
    16501 tacaatcgct ttattatctt gtattaaatt atttaacatc gatgatttac ctacgtttgg
    16561 tttaccaaca ataactgtag ataaaccttc acgcataatt ttaccctgcg caccggtatc
    16621 taataaacga ttaatttcct gtttgatttc tttagactgc tctaaaagaa attcagtagt
    16681 cgcatcttca acatcatcgt attcaggata atcaatattc acttccactt gagcgagtat
    16741 ctctaatata gattgacgtt gttttttgat taagtcactt agacgacctt caatttgatt
    16801 catcgcaact ttagaagctc tatctgtttt cgagcgaata aagtccataa ctgcttcagc
    16861 ttgagataaa tcaatacgac cattcaaaaa ggcacgtttt gtaaattcac ctggctcagc
    16921 cattctagcg ccatatgtca tagtaagttc cagcactcta ttaatcgtta aaataccacc
    16981 atgacaatta atttctataa tatcttcgcg tgtaaatgtt tttggcgctc ttaacacaga
    17041 caccataact tcttcaacca cttctttaga ctctggatca ataatatgac cgtaattaat
    17101 cgtatgtgat ggaacatcat ttaaaagatg ttttccttta tataatttgt cagcaatttc
    17161 aacagcttgc ggtccagaca atcgaacaat tccaattgcc ccttcaccca ttggtgttga
    17221 aatactcgta attgtatcta aatccatatt gctactcgcc tccttcaacg atgtgaatac
    17281 attttaaagt aagttattat aaccctaagg tcagtcttac gtttgtctga ggtaagactt
    17341 cgggatgcgt tgagtggtta atgttttcct tcccctaccc tatccttact taatcttttt
    17401 attaaaaact ttggcaattt taagtacgtg ctcaagacta ttctgtattt gtaaagtcgt
    17461 catatcttta gctggctgtc ttgctattac aataatatct ttggccaata tatgcgactt
    17521 atgtactttg aaattttcac gtattgctct tttaatcttg tttcttaaca ctgcattacc
    17581 tagtttttta gaaacactaa tacctaagcg aaaatggtct atttctttat tattacaagt
    17641 gtatacaaca aattgtctgt tggctacaga atgacctttt ttatatattc tctgaaaatc
    17701 tgcattcttt ttaattcggt aagctttttc caataacatc actcgcttat ttatcgtttt
    17761 tatttgaagc tatatttaaa cttctattga gcttataaca taaatttcta tttattctta
    17821 atttaaacga aaaaaaagat cactgatgag tcagtgatct tatgcagata aaactttacg
    17881 gcctttacga cgacggcgcg ctaaaacttt acggccattt tttgtgctca tgcgttttct
    17941 gaaaccatga actttactat gtttacgttt atttggttga taagtacgtt ttaccatgca
    18001 aaacacctcc atctttatac agttatctaa tataccagat tatgcagact ctgatcactt
    18061 taatagataa gattttgcgt ttcttagatt aaaattctcg tattagctca ttgattatct
    18121 agtcataatt caagcaacta ctacaatata acaaaatcct ttttataacg caagttcatt
    18181 ttatactact gctcaatttt tttactttta tcgattaaag atagaaatac acgatgcgag
    18241 caatcaaatt tcataacatc accatgagtt tggtccgaag catgagtgtt tacaatgttt
    18301 gaatacctta tacagttctt atacatactt tataaattat ttcccaagct gttttgatac
    18361 actcactaac agatattcta tagaaggaaa agttatccac ttatgcacat ttatagtttt
    18421 cagaattgtg gataattaga aattacacac aaagttatac tatttttagc aacatattca
    18481 caggtatttg acatatagag aactgaaaaa gtataattgt gtggataagt cgtccaactc
    18541 atgattttat aaggatttat ttattgatat ttacataaaa atactgtgca taactaataa
    18601 gcaggataaa gttatccacc gattgttatt aacttgtgga taattattaa catggtgtgt
    18661 ttagaagtta tccacggctg ttatttttgt gtataactta aaaatttaag aaagatggag
    18721 taaatttatg tcggaaaaag aaatttggga aaaagtgctt gaaattgctc aagaaaaatt
    18781 atcagctgta agttactcaa ctttcctaaa agatactgag ctttacacga ttaaagatgg
    18841 tgaagctatc gtattatcga gtattccttt taatgcaaat tggttaaatc aacaatatgc
    18901 tgaaattatc caagcaatct tatttgatgt tgtaggctat gaagttaaac ctcactttat
    18961 tactactgaa gaattagcaa attatagtaa taatgaaact gctactccaa aagaaacaac
    19021 aaaaccttct actgaaacaa ctgaggataa tcatgtgctt ggtagagagc aattcaatgc
    19081 ccataacaca tttgacactt ttgtaatcgg acctggtaac cgctttccac atgcagcaag
    19141 tttagctgta gccgaagcac cagccaaagc gtacaatcca ttatttatct atggaggggt
    19201 tggtttagga aaaacccatt taatgcatgc cattggtcat catgttttag ataataatcc
    19261 agatgccaaa gtgatttaca catcaagtga aaaattcaca aatgaattta ttaaatcaat
    19321 tcgtgataac gaaggtgaag ctttcagaga aagatatcgt aatatcgacg tcttattaat
    19381 cgatgatatt cagttcatac aaaacaaggt acaaacacaa gaagaatttt tctatacttt
    19441 taatgaattg catcagaata acaagcaaat agttatttcg agtgatcgac caccaaagga
    19501 aattgcacaa ttagaagacc gattacgttc acgctttgaa tgggggctaa ttgttgatat
    19561 tacgccacca gattatgaaa ctcgaatggc aattttgcag aagaaaattg aagaagaaaa
    19621 attagatatt ccaccagaag ctttaaatta tatagcaaat caaattcaat ctaatattcg
    19681 tgaattagaa ggtgcattaa cacgtttact tgcatattca caattattag gaaaaccaat
    19741 tacaactgaa ttaactgctg aagctttaaa agatatcatt caagcaccaa aatctaaaaa
    19801 gattaccatc caagatattc aaaaaattgt aggccagtac tataatgtta gaattgaaga
    19861 tttcagtgca aaaaaacgta caaagtcaat tgcatatccg cgtcaaatag ctatgtactt
    19921 gtctagagag cttacagatt tctcattacc taaaattggt gaagaatttg gtgggcgtga
    19981 tcatacgacc gtcattcatg ctcatgaaaa aatatctaaa gatttaaaag aagatcctat
    20041 ttttaaacaa gaagtagaga atcttgaaaa agaaataaga aatgtataag taggaaactt
    20101 tgggaaatgt aatctgttat ataacagcac taatgataac aatcattttt tacatttcta
    20161 tatgctaatg tggcaagatg agcaaaactc attttgtgga taatgtttaa aagtcataca
    20221 caccatacac aagttatcaa catgtgtata acttcgccaa atctatgttt ttaagactta
    20281 tccaccaatc cacagcacct actactatta ctaagaactt aaaacctata taattatata
    20341 taaacgactg gaaggagttt taattaatga tggaattcac tattaaaaga gattatttta
    20401 ttacacaatt aaatgacaca ttaaaagcta tttcaccaag aacaacatta cctatattaa
    20461 ctggtatcaa aatcgatgcg aaagaacatg aagttatatt aactggttca gactctgaaa
    20521 tttcaataga aatcactatt cctaaaactg tagatggcga agatattgtc aatatttcag
    20581 aaacaggctc agtagtactt cctggacgat tctttgttga tattataaaa aaattacctg
    20641 gtaaagatgt taaattatct acaaatgaac aattccagac attaattaca tcaggtcatt
    20701 ctgaatttaa tttaagtggc ttagatccag atcaatatcc tttattacct caagtttcta
    20761 gagatgacgc aattcaattg tcggtaaaag tgcttaaaaa cgtgattgca caaacaaatt
    20821 ttgcagtgtc cacctcagaa acacgcccag tactaactgg tgtgaactgg cttatacaag
    20881 aaaatgaatt aatatgcaca gcgactgact cacaccgctt ggctgtaaga aagttgcagt
    20941 tagaagatgt ttctgaaaac aaaaatgtca tcattccagg taaggcttta gctgaattaa
    21001 ataaaattat gtctgacaat gaagaagaca ttgatatctt ctttgcttca aaccaagttt
    21061 tatttaaagt tggaaatgtg aactttattt ctcgattatt agaaggacat tatcctgata
    21121 caacacgttt attccctgaa aactatgaaa ttaaattaag tatagacaat ggggagtttt
    21181 atcatgcgat tgatcgtgcc tctttattag cacgtgaagg tggtaacaac gttattaaat
    21241 taagtacagg tgatgacgtt gttgaattat cttctacatc accagaaatt ggtactgtaa
    21301 aagaagaagt tgatgcaaac gatgttgaag gtggtagcct gaaaatttca ttcaactcta
    21361 aatatatgat ggatgcttta aaagcaatcg ataatgatga ggttgaagtt gaattcttcg
    21421 gtacaatgaa accatttatt ctaaaaccaa aaggtgacga ctcggtaacg caattaattt
    21481 taccaatcag aacttactaa aaataaatat aaataaagga tgacgtgatt aattaaaacg
    21541 tcatcct
//
LOCUS       DBNENW010000035        21326 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.035, whole genome shotgun sequence.
ACCESSION   DBNENW010000035 DBNENW010000000
VERSION     DBNENW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.035"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..273
                     /locus_tag="AC1LW8_001010"
     CDS             <1..273
                     /locus_tag="AC1LW8_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP4772864.1"
                     /translation="KKNKNFLLDLMFNNKNGDTLIKDGVPKDYKVADKSGQAITYASR
                     NDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSVMKEF"
     gene            642..1175
                     /locus_tag="AC1LW8_001011"
     CDS             642..1175
                     /locus_tag="AC1LW8_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP4772865.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            1589..1882
                     /locus_tag="AC1LW8_001012"
     CDS             1589..1882
                     /locus_tag="AC1LW8_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HIP4772866.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            1957..3891
                     /locus_tag="AC1LW8_001013"
     CDS             1957..3891
                     /locus_tag="AC1LW8_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HIP4772867.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            3895..4206
                     /locus_tag="AC1LW8_001014"
     CDS             3895..4206
                     /locus_tag="AC1LW8_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIP4772868.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            4218..4844
                     /locus_tag="AC1LW8_001015"
     CDS             4218..4844
                     /locus_tag="AC1LW8_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772869.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            5082..5711
                     /gene="mobV"
                     /locus_tag="AC1LW8_001016"
                     /pseudo
     CDS             5082..5711
                     /gene="mobV"
                     /locus_tag="AC1LW8_001016"
                     /inference="COORDINATES: protein motif:HMM:NF013258.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
     gene            5805..6350
                     /locus_tag="AC1LW8_001017"
     CDS             5805..6350
                     /locus_tag="AC1LW8_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HIP4772870.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMIL
                     DDYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQEREIIFKEDREDGYTL"
     gene            complement(6392..6676)
                     /locus_tag="AC1LW8_001018"
     CDS             complement(6392..6676)
                     /locus_tag="AC1LW8_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772871.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYV
                     AKTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
     gene            6734..7594
                     /locus_tag="AC1LW8_001019"
     CDS             6734..7594
                     /locus_tag="AC1LW8_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIP4772872.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            7681..8022
                     /locus_tag="AC1LW8_001020"
     CDS             7681..8022
                     /locus_tag="AC1LW8_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4772873.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(8261..8332)
                     /locus_tag="AC1LW8_001021"
     CDS             complement(8261..8332)
                     /locus_tag="AC1LW8_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIP4772874.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
     gene            8481..8666
                     /locus_tag="AC1LW8_001022"
     CDS             8481..8666
                     /locus_tag="AC1LW8_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772875.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            complement(8885..9628)
                     /locus_tag="AC1LW8_001023"
     CDS             complement(8885..9628)
                     /locus_tag="AC1LW8_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HIP4772876.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NARQNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSA
                     NPNDNDSDIEEKSEETEAKKGFWSRLFGN"
     gene            10039..10715
                     /locus_tag="AC1LW8_001024"
                     /pseudo
     CDS             10039..10715
                     /locus_tag="AC1LW8_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            10726..11008
                     /locus_tag="AC1LW8_001025"
                     /pseudo
     CDS             10726..11008
                     /locus_tag="AC1LW8_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            12217..12834
                     /gene="cadD"
                     /locus_tag="AC1LW8_001026"
     CDS             12217..12834
                     /gene="cadD"
                     /locus_tag="AC1LW8_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HIP4772877.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            12853..13200
                     /locus_tag="AC1LW8_001027"
     CDS             12853..13200
                     /locus_tag="AC1LW8_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIP4772878.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNQEGF"
     gene            13366..13458
                     /locus_tag="AC1LW8_001028"
     CDS             13366..13458
                     /locus_tag="AC1LW8_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIP4772879.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(13630..14601)
                     /locus_tag="AC1LW8_001029"
     CDS             complement(13630..14601)
                     /locus_tag="AC1LW8_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIP4772880.1"
                     /translation="MFNAVVVRKSASNVSYKVETVDESYLSKGDVLVKVVYSSINYKD
                     MLALQYNGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILRKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNASILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            14725..15144
                     /locus_tag="AC1LW8_001030"
     CDS             14725..15144
                     /locus_tag="AC1LW8_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4772881.1"
                     /translation="MELDNCINYLLSISQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNNYFANLTNEETVVFKKILLKINETTF"
     gene            complement(15440..16255)
                     /locus_tag="AC1LW8_001031"
     CDS             complement(15440..16255)
                     /locus_tag="AC1LW8_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP4772882.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(16248..17691)
                     /locus_tag="AC1LW8_001032"
                     /pseudo
     CDS             complement(16248..17691)
                     /locus_tag="AC1LW8_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
     gene            complement(17663..18256)
                     /locus_tag="AC1LW8_001033"
     CDS             complement(17663..18256)
                     /locus_tag="AC1LW8_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP4772883.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            complement(18520..18900)
                     /gene="blaI"
                     /locus_tag="AC1LW8_001034"
     CDS             complement(18520..18900)
                     /gene="blaI"
                     /locus_tag="AC1LW8_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HIP4772884.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(18890..20647)
                     /gene="blaR1"
                     /locus_tag="AC1LW8_001035"
     CDS             complement(18890..20647)
                     /gene="blaR1"
                     /locus_tag="AC1LW8_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HIP4772885.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNGQ"
     gene            20754..>21326
                     /locus_tag="AC1LW8_001036"
     CDS             20754..>21326
                     /locus_tag="AC1LW8_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP4772886.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLS"
BASE COUNT         7664 a         2929 c         3170 g         7563 t
ORIGIN      
        1 aaaaaaaata aaaatttctt acttgattta atgtttaata ataaaaacgg agacacttta
       61 attaaagatg gtgttccaaa agactataag gttgctgata aaagtggtca agcaataaca
      121 tatgcttcta gaaatgatgt tgcttttgtt tatcctaagg gccaatctga acctattgtt
      181 ttagtcattt ttacgaataa agacaataaa agtgataagc caaatgataa gttgataagt
      241 gaaaccgcca agagtgtaat gaaggaattt taatattcta aatgcataat aaatactgat
      301 aacatcttat agtttgtatt atattttcta ttatcgttga catgtataat tttgatatca
      361 aaacctgatt ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact
      421 agaaatattg tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt
      481 catcaatcga aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag
      541 gttaaataat tctcatatat caagcaaagt gacataattg ttgaatttat agtaactgaa
      601 acgtatgatt agggtgtata ttaattatga gatggaaatc aatgattgtt ggctatgcga
      661 gagtatcatc gaacgatcaa aatttagaaa gacaacttga aaatttaaag acgttttgtg
      721 ctgaaaaaat atttacagaa aaacagtctg ggaaatctat tgaaaataga ccagtacttc
      781 aagaagcact taactttttg aggatgggag atcggtttgt agtagaatcc attgattgtt
      841 taggtcgtaa ttatgatgaa gtggttggca atccattact ggataagttt atgaaagatt
      901 taattattca aatattagca atggtttcag aacaagagag aaatgaaagt aaacgtcgac
      961 aagcgcaagg aattcaagtt gcgaaggaaa aaggcgtata taaagggcgc cccttacttt
     1021 attcaccaaa tgctaaagat ccacagaaac gtattattta tcatcgcgtt gtagaaatgt
     1081 tagaagaagg ccaatcaatt agtaaaattg cgaaagaagt aaatataaca aggcagacga
     1141 tttacagaat taaaaacgat aatgatttga tttaataaac tttgtttcaa tgcaactgag
     1201 agcttctcac agcgtctcaa aacagatgat tataatctat tatgagtttt taataataag
     1261 acaagaaaag agagcgtaaa tatgctctct ctttttctca ttaacgaacg tttataacgc
     1321 aatcatgatg agagtattaa tacagttaat tatttgaaat actttagttt atataatgtt
     1381 gaattatagg tagtgaaatt gtatttatta tacattgtca tatcgttgtt taatgatagt
     1441 taatttaagg ataatagaat taatattata tatagatgaa caaaacaagc aacagagcag
     1501 aaaaaacact tttagattat ttttactcat tctctatttt atttattttt gctataataa
     1561 ataaagaata ttaaatggag gtgtttttat gaaaaagaaa tttgtttcta gttgtattgc
     1621 tagtacaatc ttattcggca ctttattagg tgtaacttat aaagctgaag ctgcaacagt
     1681 acatgtagct ggtggtgttt ggagccatgg tattggaaaa cattacgtat ggtcttatta
     1741 tagtcacaat aaaagaaatc atggttcaac agcagtaggg aaatattcat cttttagtgg
     1801 tgttgctcga cctggtgttc aatctaaggc atctgctcca aaggcttggg gtggaaacaa
     1861 aacattttat agtcttcact aattacatat attaattaag gagaatagac agaattaagt
     1921 aaacttaatt ctgtctattc tcttgaaaga ggcaatatga aaaaattcaa attattttta
     1981 gattttagta ctttattatt aatttcaggg ctattatttt tatttttctt caaagaaaat
     2041 gaagaaatta tacctgaaag ttccaatatt ttaacaatct ctaattggga taaatctaac
     2101 agtaaatcaa aagtgttaga tgtcattgaa tcaggcgcca aaaatcaaaa tatacaaata
     2161 attaaatccg ttaaagattt tgataataaa aaagaatttt ttgttttcaa ctcaaaacga
     2221 aataattctg actttatacg taataaaaca tcacttttaa ctccatcaga tttattaaac
     2281 agagagataa aaggaaagta ttatattatt ggtgaacatt tcaatgtaga agagcttacg
     2341 agtgaattaa aaaaagctgg tttaacatct gaagttgaat acataaatag aaacatatta
     2401 ttttttgaat tagtcataga taatgattta ttagtgcctt tcatattctt aggcgtatta
     2461 tatcttttgt acttcttata tgacagagga tataatttga aattttatgc tattcaaaag
     2521 ctacatggct tttcaactac acgcattatt tttcataata ttcatataaa aatcatttat
     2581 tggctagtac ttaccgctat tttctttatt gctaatatat taatcattca tatagccaat
     2641 ttagaattgg atttcttaat gtttattcgt agatttatcg tgttgctttt tgtttttgct
     2701 tgtattacta cattgctgtg gctgatttcg tatagtcttc ttttgaagtt aagtattcca
     2761 ttaatcttaa aagggaaaaa aggatataag ttttcaatat ttatgggtac cttcaccaaa
     2821 tgtatcatgg tgctaatact atctttttta ctggcacaaa atttaaatgc ttatacgaag
     2881 ttaaagaata tatatgactc tgaaaaaatc tggcaaaagt tagataatta ctatacatta
     2941 gagatttctc ctaatattag aaattcagaa gaaaaacaaa ttttagaaac taatatatac
     3001 aatttaatta agaaaagcga acaacttggt ggattgcttt taaagaataa taatatagcc
     3061 aatcccgata aaaacaatta cattcctgaa aatggcaatg tcttttttat caataacaat
     3121 tttctgaatt tctataaaaa tataaatcat gattttaaaa tgattgacaa tcattcggat
     3181 aaaataaacg tgtttattcc tccaaggtta caatatcaaa aatctgaaat tcaaaaaaat
     3241 caccaaggat ggattgactt tcaaaagcaa caaaacaaaa aagtagatgt gcaagtactg
     3301 tcaaaaaacg tatctgtttt ttcttttgat cgcacaacta atttgaaatt tgggtatcta
     3361 gattctccaa tagtaatgtt gttgacactt gacgatttaa caggagattt ttatttagct
     3421 gctgtttctc aaggtggata tctatttaaa gatttagata cacttaaaca gttgcttaaa
     3481 gataatcacc tagaagaaga aattagcggt ataactaatt acaaggattc tgtgttaaat
     3541 gaattaaatg aaacgaaaac aaaaatgata ataacaatag ttactattat tattaacgtt
     3601 ttcattcttt tgatagctac tgtatttgaa acaattcaat attttagctg gaataaaaag
     3661 caactattat taagaaaaat tcatggttat tcattattca gcagtaatgt acgttattta
     3721 acaatttcta tcttactttc tattatgtta gcttacacta cgcatatttt atttggttca
     3781 aagatattac tattcataat aatgtctatt gctattattc aacatttact tcaaattgct
     3841 tatattaaat atcttgaaaa atactttaaa gatttaatga gggagattta aacaatgaaa
     3901 agaactttaa ttattgtaac aattgtacta ttaatcatca tacttagtct gttattggtt
     3961 agaaatgagc atttagatcg ttttaaccca ctgttaaaag aaaaagtatc ttatgccaaa
     4021 gttccaaaag atacccagga ctatagaaat ataactgtat actctaaaaa tggagaaaaa
     4081 aagagttata aattggattt tttaggttat gatcctacaa aagaatatgt gaaagtaaat
     4141 cataaaggga aatatgtgcg ttcgatagaa tacattacaa aagatattcc tagcttttta
     4201 aaatgaggtg accgatagtg ataaaacttg aaaatgtttt tgtaaaaaaa ggtaataaaa
     4261 acattttaga cggatgtaac tttaattttg aaaaaggaaa atcatatgct ttagttggag
     4321 aaagtggcgc tggaaaatct actttactta acataatcgc tgggttcgaa gatgtatcac
     4381 aaggaagcat atatattgaa gataagttac taaaaaagaa agtcgatttt tatagatata
     4441 cgttagggta tttgtttcaa aattttgctt tacttgaaaa tcaaacaatc agtcaaaatt
     4501 tagatttagc cctaaagttc aaaaagaata aaaaagataa tatgaactta aagaaagaag
     4561 tattaaaaaa ggtcggccta gatttagata tcaagagaat tgttagttct ctaagcggtg
     4621 gtgaacaaca aagagtagca ttggcaagat taatattaaa agatccgaaa atcattttag
     4681 ctgatgaacc tactgggtca ctagatacga aaaatggaaa aatagttatt gatttattat
     4741 tgaagctact tgatgaaaat aaaactatga ttgtcgtaac gcatgattta gagcttgcga
     4801 aacgatttga tgtcatagtc aatatttctg agcttagaag ttaagaaata ttgataggta
     4861 tagccataca tagagaaaaa acaatatgaa atatgattag tcattacttt tgagtttttt
     4921 aatttaagga ctggattttc cagtcctttt ttatgatgac aaaaaacaag cctcgcagag
     4981 cacacgtttt taatgactta tttggttttt aataagtcta gtatgttaaa acttttctgt
     5041 gtttaggtgt tataatcata tgcagtggaa gttgatttat tatgtcttat tcaattattc
     5101 gagttgttaa agtgaaatca aaaacaaata caagaggtat tcaaagacat attcaaagag
     5161 aaaataaaaa ttatgaaaat atagatattg atttaagtaa gtcatattta aactatgatt
     5221 tagtcaatga tactaagttt gattttaata aaaaaattga tgagaaaatc gaaaaaaatt
     5281 ataaagggaa acgcaaaatt agaacagatg ccattaaaca tattgatgga ttaattacat
     5341 cagataatgt gttttttaat caattatctg aagaagagac aaaatagttt tttaagcatg
     5401 ctaaagcgtt cttggaagaa gaatacggta aagaaaatct tttatatgcg acagtacata
     5461 tggatgagat aacaccacat atgcattatg gcgtagttcc tattactaaa gatggacgtt
     5521 taagtgcgaa agaagttgta ggtaataaaa aagcattaac cgaattccaa gatagattca
     5581 atacgtatat taataagcaa ggctatgatt taaaacgagg aatttctaga caacttacaa
     5641 aagaaaaaca tgatcaagtt agccgttata aacaaaaaac agaatatcat aaacaaatgc
     5701 atatgcgcta gaagcaaata gaggattatt taaagcaaga aaatgataaa ttgatacaaa
     5761 actaccaaaa atcattagat acgcttaaaa agcttctgag tgtcatgtat gaaattaaaa
     5821 ctaaaaatgt aggtggatgg tttcataaag agaagcaaga aacaggcaat attgtaatta
     5881 ctaaaactga ttttgaaaaa tacactaaac aaattaaagc agcacagatg atattagatg
     5941 attatgaatg tattaaaagt gggaagtcat taaaaaaagc agagaaacaa aatgaaagtt
     6001 tagttaatga attgactagt gtccatatgg aaaatgaaaa gctggttgaa gaatttaatg
     6061 atttagcaca acgttataat tatttgttgt cagagaatga aaagaaagat aaagaactaa
     6121 attatacgct taagttattc aatcaagttt ttaaaatcat taaaagtatg atgaaagaag
     6181 agcgttatca tacactaata aatcatattg ataatcattt agacaattct aaaatacgtg
     6241 aagtaatgac gatagataac aatgatgaac agttctttaa gaagaaatat caagcacaag
     6301 aacgagaaat catttttaaa gaagatagag aagatggtta tacattataa aaatagatat
     6361 ttaaatgcat ttgtttgagg ttaattattt tttaggatga aaaataggta gcccccctcg
     6421 aactttttaa tatattttta taaatctttt taaaaacctt tttaggggct tgttcagccc
     6481 tagagcaaca agggatttca atgcctaaag gagtgttttt gccacataaa aggagtgttt
     6541 ttgccacata aaaggagtgt ttttgccaca taaaaggagt gtttttgcca cataaaagga
     6601 gtgttttaaa aagttgtact ccttttatgg tttgacattg ataataaagt agtttattgt
     6661 gaataaaagt tttcaaatgg gagtgtatat tcaaacgata ctcctttagg ttcgtaataa
     6721 agggggctgt atcatgacag gagagacagt tgtatataaa aatgaaatga atttagttcc
     6781 attaaggcga tttacagcta ctgaaattaa tttatttttt gcgatgtgta ataaattaaa
     6841 agagcaagat actaatacat tacgtttgtc ttttgatgaa ttaaaaaaat taagtaatta
     6901 tagtccagaa acacgtaata ttaatagatt tgctaatgat ttagataatg tgtataaaaa
     6961 gatgttgaat ttaaccatta gatatgaaga tgatgatgta atagaaagat tcgttttatt
     7021 caatcattat agaatccata agcgagaaca atatctagaa atatcaactt cttcaaattt
     7081 aaaacacata ttgaattcaa ttactaataa ttttacaaaa tttgaattaa aagaaatgac
     7141 taggcttaaa tctacatatt ctaaaaatat gtttagatta cttaaacaat acaaacatac
     7201 aggctattta aaaattcaca tcgatgattt taaaaatcgt ttagatatac ctaaatctta
     7261 tcgtatgact gatattaaca aaaatgtttt taaacctatt attattgaat taggttctat
     7321 atttaataat cttactatca ataaaatcaa agcgaaaaaa gggcgtaaaa ttgaatggat
     7381 agagttcaca tttgacgctg agaaacgcat tcataacaaa cgacaacctc aaatgtccaa
     7441 gatagataaa tcaagacaat atgtgaggcg tgaaaagacg cctaaatggt tagaagaacg
     7501 gtcatatgaa aagcaacctc aaaaagatta cgacccacaa ttagagaaag aacgagagga
     7561 ttttttaaaa caacttgaac tgaattggga ataacgcaac ttttcgttgc gagttggtta
     7621 actaaaagta tcgtgacttt tcaattattt taattatagt ttgagttaag gagtgatttt
     7681 atgaaattag ctgaagctat aaaagaacag agagaattaa aaggatggtc tcaagaggaa
     7741 ttagccaata tattaaaggt ttccagacaa agtgtttcta aatgggaaag tgccaaaaac
     7801 tatccttcct tagatatttt gattgctatg agcgatttat tcggaatctc tttagagcat
     7861 ttaattaaag gagatgcacg ttttaaaaaa gttattttag agggaaatta tagagagagt
     7921 aacagggtac cttccatagt tgattttctt tatgattttt ggtggctttt ctttcctgtt
     7981 gccggatttt tagtttgggc cattcattcg ttcatgggtt gattttaaaa tcaaaattta
     8041 atgcacatca ggcaatatga aaaaggaaag tatttttacg acaccttgaa atgtattgat
     8101 aagaatagaa gagtttacaa aaggcaatat gaaatatata ctaaaagtaa caagaatttc
     8161 gtttgtaaac gcaccaaacc ccgaactatt cgccgtagtt cggggttttt tggtgaattg
     8221 gctttaatcg tctatattat tttttatcac gtttatggag ctaataacca aataacgtaa
     8281 cgagacaacc actgattgcc gttgtcatga tgtgaacaag aatttcattc ataaatcgca
     8341 cctcctctct acgccgaatt gacatctgag agatagacga ccttcacatt ataacaaatc
     8401 tctccttcca tgaaataaca tgaatattaa taaatgttgt tatatattaa agataaataa
     8461 atataagggt gggattgata atgtttggcg atcaaagaca agaagctaca aaatatgtca
     8521 ttaaagaagg ttatcaagat atttatttcc tcaacaaaaa cggggaatgg tattatttcg
     8581 aagtaagaag tgtctggcgt ggcaaacata ttattagagt taaagatggt ttgttaggtt
     8641 ggagaaaaga aatagtaact gaataaagat acaaaatact tgccaatgca aaagaatgaa
     8701 aacgcgtcaa atcaacgatg tataaaagat gcataaaaga taatgaaagt aaaaagccat
     8761 aaatgccgtc atataggcat ttatggcttt ttgtttggtt aatataatcg atgattatca
     8821 gaagttcaat tcacataaaa aagcacggct aatttatgaa cttgtcgtac ctttttaatt
     8881 aaaattagtt accaaataga cgactccaaa accccttttt agcttccgtt tcttcactct
     8941 tttcttctat atcactatca ttgtcatttg gattagctga aacgtcttct ctttgtgctt
     9001 cttcaccttg tgtactttcg tcgctttgac ttccagatat atccttatat tgaatctctg
     9061 tttctttagt ttcttcctga tctcggttaa tatttacagg actagtagta tatgtttttt
     9121 cttgtgcatc aacgttttgt ctagcgttag tagatgtatc aaagggataa ttaaggtgtt
     9181 tttctttttc taattgattt tctaattttt gaatcttttt attactctct aatgccagaa
     9241 cttgctgatt ctcaagcaat tttgatcttt cttgcaactc ttttctgtaa aattcaattg
     9301 ttgtttcatg acgactttct tgtttttcaa tttgattatt taaagtatta atctgatttt
     9361 taagtatttc tactatttga gcatcgcttt tagattcaaa attaatatta tccgactctt
     9421 ttttttgttt gtcttcagta gcactttcaa aaccatattt ctttttattt tcatttactc
     9481 tttgaatgat tttttctata tcggtatctt ctttaataaa cgaagtgttt tcctgtttta
     9541 ttgtctctat attcaatctc ttaatattat taaaaatcgt ttgcttacta acccctaatt
     9601 cttcactcaa cctttttaca cttttcatat catcaaccct ttataaaatg tttataaacc
     9661 cttagtaaaa tattataagc ttataacgat taataataaa gtaatcgaca aataaaaata
     9721 taaattcgcc aaccttttta tcaagggttt gtaaagggtt tatggtgttt atcaaaggtt
     9781 tataaaccta tattttccct tttttaagtt tattcaaagt gctataataa ccttaatata
     9841 acaaaaaccc caactactgg taatagtcgg ggtttggtaa taaaacgttt agaaactgtt
     9901 tatttatata attttcgata cttagattat ataatgaata atatcttagt gcaagcacct
     9961 agattattaa ataaacacaa aaaatgtaag cgttttatta cctcttacca aactaaaatt
    10021 cggaaagagg tttttattat gtcaattaaa ctttacaaca taaatgagga atacagagaa
    10081 aagttttatc aaataccgaa agtatttttt acaaatccta aatatatcaa gttatccaat
    10141 gatgcaaaaa tggcatgggg tattcttaga gatcgtttag atttatcaat taaaaatggt
    10201 tgggttgatt ctaaaacagg taatatttat ttttattaca aaaatgaaaa cccacaaaat
    10261 atattaaatt gtaaaaaaga taaagtaatt aaaataaaga aagaacttaa ttgaagccga
    10321 attactacta caagaaagac aaggtttaaa taagacaaat aaactttatt tagttaaacc
    10381 agaagtaaca gaagatgata tttataaaat tcaaaagcaa gaaaacactg ttgagagcag
    10441 caatggcaag gaagtcggaa aaaccgactt caagaagtcg ataaaacaga atctaatgat
    10501 actgaactta gctatactga atctaacaat actgaaataa atgatatgaa tgacttgaat
    10561 gatatagagt ccgaaaatga aattagtaat cattcaagtc aatgcaaggt tatttaatgc
    10621 aaacaatttt aactgaattt gaagaaacac atgcacttta tatgcgccgt aaaaacatga
    10681 aacaatataa tatctttaat caatcattca aatgatttta taacaatcaa agatacatca
    10741 aaatatttcg ttaaatcatc tgacttttta actgatagcg atgaaaaaat caagaaattg
    10801 tcagtgatct agaaaaagat ttgtatcgaa aacgtatgtt aagttatggt ggattgctta
    10861 aataaaaaca taaaatttta aacttagacg atgctgaaga tggcaattta attaatacaa
    10921 gtgatgaaga taaaatagca aatgaagaag aaaaggcaca ttcgattacg gcaattttga
    10981 aaagcaaaat tattatttaa ggcattagtg ataactgatg tcttttttta ttactttttt
    11041 gtaaaaaaag tgctgtctta tttttgtgac aaatgctgta tgtagtgtca caaaagtgtg
    11101 acacttgagc tttttatgac cccacacaaa aacattttcg aagccttgaa atatctggct
    11161 ttgccagatc ccccattcat tgatggggac aaatttccct tatgctctta cggagatttt
    11221 agagttaaat taaaatttct aagtttttga taagaaaagc cctgcaggaa tttagaagaa
    11281 cgtggggaag ttttaaagaa ttttatgcta atttttaatt tgcatgtaaa tgggcagtgt
    11341 ctaaaaaata gttattgttt ttgctcaaat ttttgttttt gaaaatagca tatatttatt
    11401 tggctcatat ttgcgtttta aaagcgtata taagttttag ataaaactat atgaatcacc
    11461 cctaaatctt taaaatgccc tcttaaaatt caaaataagg gcataaaaaa taaaagcccc
    11521 cccagataaa gtgaggtagc tttttctttc gacgccgtat cgtcgatgta attcaaaaaa
    11581 gttatgggct ataaatctac atcacttttt caagaatgta gtagcaatat tcaacgcaaa
    11641 ttaattttta taactgctcg gaatatctca agccgtttct ttaattttga aataaaaaaa
    11701 tcgacgaagg tcgattacgt ttttgcacat gtcgatttat ccgacgtata agtgcgccct
    11761 tacgggattt aactagatta taacgacgaa ttttagacct gtaaagcaat aacaattagg
    11821 tctaccaggt agataaagtg tacaaagtag aaccgaaaaa gttatttatt ttgaagattt
    11881 ttcgtagata taatttgcta ataaacttcc taaaactgaa taaagaaaaa tagttaaact
    11941 catgtaatca ctccataatt ctgaagtgtc tccatgtcta cgcatagaca cgcatttatg
    12001 ccgagaaaat ttattgtgcg ttgagaagaa cccttaacta aacttgcaga cgaatgtcgg
    12061 catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa gggttccgag
    12121 gctcaacgtc aataaagcaa ttggaataaa gcacctatga ttatttaaaa atataagtta
    12181 taatacattc aaaaatattt ttgaacgagg tgtattatga ttcaaacggt tgttgctgct
    12241 gctgttcttt atattgctac agcagttgat ttattagtaa ttttattaat attttttgct
    12301 agagcaaaga ctagaaaaga atatagaaat atttatgttg gtcaatattt agggtctatt
    12361 attttaatat tagttagttt gtttttagct tttgtattaa attatgttcc agaaaagtgg
    12421 atattaggtt tattgggttt aataccaatt tatcttggaa ttaaagtggc tatttatgat
    12481 gattgtgaag gagaaaagag agctaaaaaa gaattgaatg aaaaaggatt gtctaaatta
    12541 gttggtacgg ttgcaattgt tacgatagca agttgtggcg ccgataatat tggtttattt
    12601 gttccttatt ttgtgacatt aagtgttact aatttattac ttactctgtt tgtcttttta
    12661 attttaattt tcttcttggt gtttactgca caaaaattag ctaatatccc aggaattgga
    12721 gaaattgttg agaaatttag tcgttggatt atggctatca tttatatagc tttaggttta
    12781 tttattatta ttgaaaatga cactattcaa acaattttag gatttatatt ttaatttagg
    12841 gtgtgatttt atatgagtta tgaaaatact tgtgatgtga tctgtgtaca tgaggataaa
    12901 gttaacaatg ctttaagttt tttagaagat gataaatcta agaaattact taacatttta
    12961 gaaaaaattt gtgatgagaa gaaattgaaa atcatattat ctttgattaa agaagatgag
    13021 ttgtgtgttt gtgatatttc tttgatattg aaaatgagtg ttgcttcaac ttcacatcat
    13081 ttaaggcttt tatataaaaa tgaggtactt gatttttata aagatggaaa gatggcatat
    13141 tattttatta aagacgatga aataagagag tttttctcta aaaatcagga gggtttttga
    13201 aacgagtgaa acgagtttct tcttgtcttg atactataaa aaacaaagtt atataacata
    13261 agacgactta tcggaagtag atatgcttgt atattcacag atggtataat aatagagtcg
    13321 cctatctctc aggcgtcaat tgacgaagag aggaggtgca ttactttgct aatcattttc
    13381 gttcacatca tagcaccagt catcagtggc tgtgctgttg cgtattatac ttattggctt
    13441 agtaaacgca acaaataatt aataggcgac tttataagcc agcatcgaaa aaatccccac
    13501 actactgcaa tagtgtgggg attggtgcat taattgttaa tcattttcgt tatataaatt
    13561 ataacactgg ttagtagaga aatgcaaaat gattgaatta atagtaaatg tattaatgaa
    13621 tactcatact tatactttta aaatagttct tcctaaatgt tgtcctttta aaagctgatc
    13681 tatcgtttta tagaattctt caaatccaat ttcattgtat agaagtgaat ttcctatatt
    13741 ccattcattt gctaacttac tccatatatc ttcacggata ttatgatcaa catatacaga
    13801 atcaatgcca attaatttaa tacctctaag aataaacggt aatatactag cgttaagtga
    13861 atttcctgct acatttccac atacagtagc aacaccttga taatttagac gttttaaacc
    13921 gtataatgtt acattactcc ccactgtatc taggaaataa tcaaaaatac cttttttaag
    13981 tggtttatta tcatcttcaa ttattattac ttgttttgca cctaattttt ttgcgatttc
    14041 tatttgcttt tcttttctta cgatagcagt gatattttca tatccaattt tacgtaaaat
    14101 ttgtatagca acactgccca caccacctgt agccccagac actaaaattt caggttgtga
    14161 tttgatattc attccatatt tttctagttc aaaaattgat aatgctgccg taaaaccggc
    14221 tgtgccatat accatagact ctttaataga catttcttgg ggaataggaa caatccactc
    14281 tccaggcact tgtgcatatt ctgaaaatcc acctgtatgt gtcacgccga catcataacc
    14341 agtaactaat acattatcgc cttctttaaa cttatcattc gaacttgatt cgacaatacc
    14401 tgcaaaatct ataccaggaa tcataggata atctctaatt acaccgccgt tatattgtaa
    14461 agctagcata tccttataat ttattgaaga gtataccact tttactaaga catcaccctt
    14521 cgataaataa gattcatcaa cggtttctac tttataacta acatttgatg cactttttct
    14581 cactactacc gcattgaaca tttattagcc ttctttcaca attgttttgt agaggtatgt
    14641 ttttgttgca aaactatttt cctacaaaac ttatcatgat atgataagcg agtcaatact
    14701 aatgatttaa gagaggtttt agttgtggaa ttagataatt gtataaatta tttgttgagt
    14761 atttctcaga atagagtgtt taagcatttt aaaagtttat taagtcccta taatattact
    14821 ccagcagaat atggtgtttt gaattgcttg attaatagta atttgaatac acctaaagcg
    14881 attggtaata ctttaaattt agaaccttca acaatatctg gaatattaga taagatgtcc
    14941 aaaaaggatc taataacacg tcaaactgat aatgaaaata gaagaactgt gctaataaac
    15001 agtacaaata aagcaaaaga cttatggcca acattagaaa agaaagccta tcaacttaat
    15061 aataattact tcgctaattt aactaatgaa gaaactgtag tgtttaaaaa gatattacta
    15121 aagataaatg aaactacgtt ttaattttta cgttattaaa acgttgaagt gcgaattata
    15181 atcgagtaaa tataaatgat agcttagctt aattatatat taaagccaac tatactttta
    15241 ttcctgaaaa acctactatc caaattttta agatacttta gcctctataa taaaaatatt
    15301 gtaatataat ttatgtattt tttaggtaca ccctaatcat acatatataa ttgttgtcac
    15361 tttactcgat atatgagaat gactttacct aataaatgag aaaagcaacg cactatatta
    15421 aaatgcgttg ctttttctat tatcgaatac ctataactaa actatctcta gctgtttcta
    15481 caacttctgt acttatttta tctaaaccat ttatatccat aattctgtct atctgcgcaa
    15541 ataaacgatg aatcagccta aaattccctt ttgtaatttt aataatcgtc gttattgcct
    15601 cataatcagt aaagtcttcg agttttaaat caaatcctaa gtcttgccac ttatattcta
    15661 atatatgatg cgtctcatct ttacttagat tatcaaattc atgcgcaaaa cctatacgcg
    15721 aatatagttg aggataacga gatagttttt tctctatacc cggcatacca ataaatatca
    15781 ttgctaaatt atgttcatca taaatagctc ttaattgctc taaatgttgc actttaaggc
    15841 gatcaatctc atctactata attaaatcaa tatatttaaa catatcacta taatgtttac
    15901 tatgttcatg tccatactta tttacaacat gtaatttttg acccaaatca atgattgcag
    15961 taatactata taaatcattt cttaatttag tttgtttttc tgctggtgca ttataaaata
    16021 ttttatccac cgatagtatg tcatcggtcg ttttactagc taaatcttcc caacctctat
    16081 ggttaacttg tttttcaata gtatcccaat ttgtgtaata cctagacgat aaagtttttc
    16141 cgacaccagg ttgaccataa catataccaa tatactgata tttaatacaa gcatcacaaa
    16201 attcagcaaa gcgtttatac tctttcgttt caatgaaatt ttgattttta gtcattacga
    16261 tatctcttta attttttgac gtttttagtc gtttcttgag gtgaataacc acaatttttt
    16321 tcttccttaa ttaattccgt ggtacttgga ctagcaatat tttgttttaa gtgttttctt
    16381 ctctgactac gagcgtgctg tatgtctttt atatctatgg agtaatcagc tatttctgga
    16441 catatagcag tacaaagaaa ttcatctcta taaaacacac gtatttcagc catatcattt
    16501 gggttataac gaatcagtac atattcacct acataagctt ctagattagt attactgtat
    16561 cgaaaacctt gaaaatgaat gccatctgaa tgaacttttc tagatttagg tatttccaac
    16621 aataataaat caagttgttc taagctactt ggcatattag ggaaaaagtg attactattc
    16681 caatgactga taggcgtggt atgaatagta ctatgttctt tttgattata atcttcaatt
    16741 aaaaagtaac gtaatttttc ttcaaaattg tgaaaattta ttagattact aggagtatca
    16801 ttattgttta tatatccagg cagttgttct aagaaagttt gattaactgt ctgaaagaat
    16861 cgttctattt ttccacgacc acgaggaaca ccaactttcg aaaacatcaa gttaattttc
    16921 aaatcaatag cgacttgttc catatgatgt gaagtaaagt cacttccatg atctgtgtag
    16981 aatttttcgg gtataccaca cactggccaa ttggtatcat ttttattcca tattgcttgg
    17041 tgtagtgtta atgctgtgtt ttgagcattg gggggcatca aaacttaaga aatagcctgc
    17101 aattgcacga ctatagtcat ccataataat agttagccat ggtctattga tattaccttt
    17161 ttggtctaat ataaagatat ctagcaaagt atggtctgct tgccatattt cattgggtct
    17221 cgaggattct ctaatttgta ttaaatcgta cttattttga tagtattttt caccctgatg
    17281 agagaaatca ataacagact taggcatcgc tttaataaca ctataaattt gtttataact
    17341 tggtatatca aaattatttt ctttacagta attagtgatt ttcctatgaa ttgtggctat
    17401 actatttctt ttattcataa gcgcaaggcg ttcaatttca tcaattatct ttggttctat
    17461 tttacgtgtt cctttatcag ctcttgtcgc atagattagt cctttaagtc cttgttcttt
    17521 atatgactta ttccacctat aaagggtgct taacgcaatc cctttacttt tagaaatact
    17581 tgataaagat tgttcgccta ggataaaagg ttttataata ttgtactttt gagtagcttt
    17641 tttacgtttg gcttcagaaa aattagttag atacttctcc ttgtttttca tcttccttgt
    17701 cctccaattt attgaggtaa cgataaattg ttgtacgtga aacatgccac tgttctgcaa
    17761 ttgtcttaat tggcgtgcca ttatcataaa gggtcttaag taagtttaaa tctttttgat
    17821 ttaatttttc tggtctgcct ccatatcggc ctctagcacg tgctgcaatt cggccagctg
    17881 aagaacgctc taaaattaaa ttacgttcaa attctgcaaa tgctgcgaat aaatgaaata
    17941 ataattgtcc agttgaactt gatttatcca ttgttatatt ttcttctaaa ctatggaaac
    18001 ttacacctcg ttcgttaagc tcattaacta atgtgattaa atcctccata ttacgtccca
    18061 atctatctaa tctccaaaca acaatcgtat ctcctgacct cgcaaattca attgctttat
    18121 ctaaaccagg tcttttactt tttgaaccac tcatatggtc attaaatatc ttttcacaac
    18181 catatgtatt taatcgatcc tcctgtaaat ttaaattttg taatccagtt gaaactcttg
    18241 cataacctat tttcaaaata caaacctcct atatacatct tcagtgtacc ataacttaat
    18301 tacaaggtct tttatgaaca tagatttttt gttgggtttt tgaacacata atcctatgta
    18361 attcttactt atttagacat aaaagaagag tgtacagaaa atggttattt tctgtacact
    18421 ctcatcttta ataatatatt ttaaatgtca ttaacaaaac taatttaata agagtcaagc
    18481 atagtttaca tatgtattat gtttgaaaca tacctctaat tactttttac taatatcatt
    18541 taaaatatct cgcaattctt caatttcttt gttatttaat tcttcatttt tcgcaaaatt
    18601 caacactaaa cttttcatat cccctccata cagtttatta agaaaggttt tagcagtttt
    18661 cattttaata tcgtcttctt taatatttga tgagtaaaaa taaatatttt ctgatttgta
    18721 tcgttttata atctcttttt tatatagtct tgtgattaat gttctaatcg ttttatcact
    18781 aacttcttta tatttttgaa tttcaactac aatttcatta gctgatactg attttttatc
    18841 ccatattata ttcataacat cccattcagc catagatatt tcaacttgct tattggccat
    18901 ttaaaacacc cattcctttt aatatttttt cacttattaa ttcagcattt ttcccagacg
    18961 ggtttccatc tgataaatgt gtagcaaaat aatacttatc atgatttgta attacgtaac
    19021 ctacaaacca cccattatta tacttcccgt ttactatacc tgtacctgtt ttcccataca
    19081 gttcatactt ttcatttttc ttaatcaata atgaagaaga taattgattc tttgcttttt
    19141 tactaaaatg gttattttgt tccatcatat ttttaaaaac tattacttgt tcaagattag
    19201 atattttcaa actatcttcc atccaatagc ttttataact tcccaaattt tcattaccat
    19261 aatttaattg cttgagttga gccgcagtat agttctttgg tatttgatta ctaatacgtt
    19321 cgaagtacca attaactgaa ttttgcattg ctgtatttaa atcttgttcc ttattccaag
    19381 actcgaaaag ataatgttta tgattccatg acatacgtga atttttatca cttataatat
    19441 gacggtcaag tccaaacagt gctaaataaa ttttgtaagt tgaatcagga gaatatcttt
    19501 ttctactttc tttttcatta taaatatagt atttgtcttt cttcatgctg tacataacga
    19561 aagatccact atttgaacca aaatttttac tttcatctaa aatttggtaa tcgctttgta
    19621 agggcttttt ataattataa tcagttaagg attgccccat taaaaactgg ctttgaatta
    19681 tcatgataaa aaaagtaaaa atacaaataa aaattagaat taattttgac tgctttttca
    19741 gatcggcttc ttttatatta attaatctat ttttgagtaa tgatttttta ccattaaatg
    19801 aatggcttaa tatatttttg ttgaaaaatg aggtttttaa tacggagtcc ataatagatt
    19861 ccgcataagt tttaaattca tttttgttaa tattattaag gacaaatcta tcggcttcta
    19921 cttcattgtc atgaattatc tttcttttta caatatgtac taatggattg taactcataa
    19981 taatagagaa gatattaaag ataattaaat gtaaagtatc tctattttta gcatgagcat
    20041 attcatgcag tattatatat tttagtcttt tatcaattac acttttaaaa tatgaactag
    20101 gaatcaaaat aatatatttc ccataccaaa aagttattgg agattgaata gcctctgctt
    20161 ttcgaatcac aatatttttt ttatattgat ggttgaaaag tatcgtatct attttatttt
    20221 tttcattttc gtttagataa agtgactgtt tctttaaata tttaagatat aataaggctt
    20281 tcaaaaattt aaaacttaaa ataataacta aaactatcca aataactgtg cagatattat
    20341 caattgaatc ccaattaaac ttatggatat ctgttgcgaa ctcttgaaca ggtttggtgg
    20401 taattatgtt atggttcaag tcgtgtgact tactttcaac tgtgggcgct tgattattca
    20461 cattattaaa ttttataaaa gagaatttaa ttgggataaa aggaattaat ccagcaagca
    20521 cagttagata ccaaacttta taatttaaac tataattaaa atagcgtttt aatatgtacc
    20581 taaaaaatac taacaataga aatataaaac aaaagcttac tacactcatt attagtaatt
    20641 tagccatttt gacaccttct ttcaaatatt tataataaac aattgacatc aatattacaa
    20701 ttgtaatatt attgttttgt aaaaattaca actgtaatat cggagggttt attttgaaaa
    20761 agttaatatt tttaattgta atggctttag ttttaagtgc atgtaattca aacagttcac
    20821 atgccaaaga gttaaatgat ttagaaaaaa aatataatgc tcatattggt gtttatgctt
    20881 tagatactaa aagtggtaag gaagtaaaat ttaattcaga taagagattt gcctatgctt
    20941 cgacttcaaa agcgataaat agtgctattt tgttagaaca agtaccttat aataagttaa
    21001 ataaaaaagt acatattaac aaagatgata tagttgctta ttctcctatt ttagaaaaat
    21061 atgtaggaaa agacatcact ttaaaagaac ttattgaggc ttcaatgaca tatagtgata
    21121 atacagcaaa caataaaatt ataaaagaaa tcggtggaat caaaaaagtt aaacaacgtc
    21181 taaaagaact aggagataaa gtaacaaatc cagttagata tgagatagaa ttaaattact
    21241 attcgccaaa gagcaaaaaa gatacttcaa cgcctgctgc tttcggcaag actttaaata
    21301 aacttatcgc aaatggaaaa ttaagc
//
LOCUS       DBNENW010000036        20690 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNENW010000036 DBNENW010000000
VERSION     DBNENW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.036"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            66..1199
                     /locus_tag="AC1LW8_001037"
     CDS             66..1199
                     /locus_tag="AC1LW8_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptidase T"
                     /protein_id="HIP4772887.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            1267..2673
                     /gene="gndA"
                     /locus_tag="AC1LW8_001038"
     CDS             1267..2673
                     /gene="gndA"
                     /locus_tag="AC1LW8_001038"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIP4772888.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2892..3263
                     /locus_tag="AC1LW8_001039"
     CDS             2892..3263
                     /locus_tag="AC1LW8_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4772889.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            3202..3456
                     /locus_tag="AC1LW8_001040"
     CDS             3202..3456
                     /locus_tag="AC1LW8_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772890.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTYILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            3457..4476
                     /locus_tag="AC1LW8_001041"
     CDS             3457..4476
                     /locus_tag="AC1LW8_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP4772891.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNERKVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            4492..6141
                     /locus_tag="AC1LW8_001042"
     CDS             4492..6141
                     /locus_tag="AC1LW8_001042"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIP4772892.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(6223..7089)
                     /locus_tag="AC1LW8_001043"
     CDS             complement(6223..7089)
                     /locus_tag="AC1LW8_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HIP4772893.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERTPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7319..8803)
                     /gene="zwf"
                     /locus_tag="AC1LW8_001044"
     CDS             complement(7319..8803)
                     /gene="zwf"
                     /locus_tag="AC1LW8_001044"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIP4772894.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNFDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            9116..10036
                     /gene="rnz"
                     /locus_tag="AC1LW8_001045"
     CDS             9116..10036
                     /gene="rnz"
                     /locus_tag="AC1LW8_001045"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIP4772895.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGVPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            complement(10139..10954)
                     /gene="proC"
                     /locus_tag="AC1LW8_001046"
     CDS             complement(10139..10954)
                     /gene="proC"
                     /locus_tag="AC1LW8_001046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIP4772896.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            11096..11851
                     /locus_tag="AC1LW8_001047"
     CDS             11096..11851
                     /locus_tag="AC1LW8_001047"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP4772897.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELEKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKRMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            11867..12115
                     /locus_tag="AC1LW8_001048"
     CDS             11867..12115
                     /locus_tag="AC1LW8_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772898.1"
                     /translation="MNCQSESVVRFCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(12331..13239)
                     /locus_tag="AC1LW8_001049"
     CDS             complement(12331..13239)
                     /locus_tag="AC1LW8_001049"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4772899.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTKWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            13321..13863
                     /locus_tag="AC1LW8_001050"
     CDS             13321..13863
                     /locus_tag="AC1LW8_001050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP4772900.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            13968..14417
                     /locus_tag="AC1LW8_001051"
     CDS             13968..14417
                     /locus_tag="AC1LW8_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIP4772901.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            14466..15353
                     /gene="xerD"
                     /locus_tag="AC1LW8_001052"
     CDS             14466..15353
                     /gene="xerD"
                     /locus_tag="AC1LW8_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIP4772902.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(15431..15937)
                     /locus_tag="AC1LW8_001053"
     CDS             complement(15431..15937)
                     /locus_tag="AC1LW8_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIP4772903.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNQETSTDPFIVDHHMRRDRSEVISAKEEAI
                     QLRKHRRN"
     gene            16029..16760
                     /locus_tag="AC1LW8_001054"
     CDS             16029..16760
                     /locus_tag="AC1LW8_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIP4772904.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHILAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            16753..17295
                     /gene="scpB"
                     /locus_tag="AC1LW8_001055"
     CDS             16753..17295
                     /gene="scpB"
                     /locus_tag="AC1LW8_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIP4772905.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKNQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            17288..18025
                     /locus_tag="AC1LW8_001056"
     CDS             17288..18025
                     /locus_tag="AC1LW8_001056"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP4772906.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            18158..18883
                     /gene="srrA"
                     /locus_tag="AC1LW8_001057"
     CDS             18158..18883
                     /gene="srrA"
                     /locus_tag="AC1LW8_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HIP4772907.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTLEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            18864..20615
                     /gene="srrB"
                     /locus_tag="AC1LW8_001058"
     CDS             18864..20615
                     /gene="srrB"
                     /locus_tag="AC1LW8_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HIP4772908.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
BASE COUNT         7286 a         2986 c         3806 g         6612 t
ORIGIN      
        1 acattttatt tcatcaattt ttaccgtttg gcgtacatga aaattttgat aaaaggggtt
       61 ccattatgat taatgagcaa agattactta atacgttttt tgaacttgta caaatcgatt
      121 ctgagacagg gaatgaatca acaatccaac ctattttaaa agaaaaattc atagcattag
      181 ggttagatgt taaagaagat gaagcggcta aacatcctaa attaggtgcc aataatctag
      241 tgtgtacaat gaatagtact atcgaagaag gcgaagtacc taaattatat ttgacgagcc
      301 atatggatac tgttgtaccc gcaattaatg taaagccaat tgtaaaagat gacggctaca
      361 tatactctga tggtacgact atactaggtg cagatgataa agcaggatta gcagcgatgc
      421 ttgaagtttt gcaagtgata aaggaacaac aaatcccaca tggacaaatt caatttgtga
      481 ttactgttgg ggaagaatca ggtttaattg gtgctaaaga attaaattca gagttgttgg
      541 acgcggactt tggttatgct attgatgcaa gtgctgatgt cggcactacg gttgtaggtg
      601 caccgacgca aatgttaatt tcagctaaaa tttttggcaa aacggctcat gcaagtacgc
      661 caaaagaggg tgttagtgct attaatattg cggcgaaagc aattagtcga atgaaattag
      721 gacaagttga tgaaataact actgccaata ttggtaagtt tcatggcggt tcagctacga
      781 atattgttgc agatgaagtc attttagaag cagaggcacg ttctcacgat ccagaaagaa
      841 taaaaacaca agtcaaacat atgactgatg tgtttgaaac tactgcatca gaattaggtg
      901 ggaaagcaga agttacagtg gagcaaagtt atccaggatt taaaattaat gataatgaag
      961 cggtagttaa aattgcacaa gaaagtgctc gtaatttagg attatctgca aatacaatta
     1021 tttctggtgg cggttcagac ggcagtatta ttaatacatt tggtattcct tcagtcattt
     1081 taggagtggg ttatgaaaaa atccatacaa caaatgagag aatgccgatt aaatcattga
     1141 acttgcttgc aagtcaagtg ttagaaataa tcaaaatagt tgcgcgacaa tcgaaatgat
     1201 ttagtgtagt gtatgtctat tttgtgatac aatactaatg aaaaatcaac aagtgaggta
     1261 agtaatatga cacaacaaat tggagttatt ggtttagccg ttatgggtaa aaacctagct
     1321 tggaatattg aatcacgcgg atatagtgta tctgtattta accgctcaag tgaaaaaact
     1381 gatttaatgg ttgaagaatc aaaagggaaa aatattcatc caacgtactc attggaagaa
     1441 tttgttaatt ctttagaaaa accacgtaaa attttattaa tggttcaagc aggtaaagct
     1501 acggatgcaa cgattgatag tttgttacct ttattagatg atggagatat tttaattgat
     1561 ggtggtaata caaactatca agatactatc agacgtaata aagcattagc acaaagtgca
     1621 atcaacttta ttggtatggg cgtttctggt ggtgaaattg gagcgttaac tggtccgtca
     1681 ttaatgccgg gtggacaaga agaagcttat aacaaagttg cagatattct tgatgcaatt
     1741 gctgcaaagg caaaagacgg tgcttcatgt gttacttata ttggaccaaa tggtgctgga
     1801 cactatgtga aaatggtaca taatggtatt gaatatgctg acatgcaatt aattgcagaa
     1861 agttatgcta tgatgaaaga attattaggc atgtctcatg aagacattgc tcaaacgttc
     1921 aaagactgga atgctggtga attagaaagt tatttaattg aaatcactgg tgatattttc
     1981 atgaaattag atgaaaataa agaagcatta gttgaaaaaa ttcttgatac tgcaggtcaa
     2041 aaaggtacag gaaaatggac ttcaattaac gcattagaat taggtattcc attaacaatc
     2101 attactgaat ctgtatttgc acgttttatc tcttcaatca aagaagagcg cgtaaatgct
     2161 tctaaagaat taaatggtcc aaaagcatca tttgatggtg acaagaaaga ctttttagaa
     2221 aaaattcgta aagcattata tatgagtaaa atttgttctt atgcacaagg tttcgcgcaa
     2281 atgcgtaaag caagtgaaga taatgaatgg aatttaaaac ttggtgattt agctatgatt
     2341 tggagagaag gttgcattat ccgtgcacaa ttcttacaaa agatcaaaga tgcttacgat
     2401 aataatccag gattacaaaa cttattgcta gatccttact ttaaaaacat cgttactgaa
     2461 tatcaagatg cgttacgtga tgttgtcgca actggtgttc aaaatggtgt accaacacct
     2521 ggtttctcat caagtattaa ttactatgat agttaccgtg cagctgattt acctgcaaac
     2581 ttaattcaag cacaacgtga ctacttcggt gctcatacgt acgaaagaaa agataaagaa
     2641 ggcgtattcc atacacaatg gattgaagaa taatattgat taaaggatag ttgaaagata
     2701 ctatcctttt ttatatcttt tgataaatat aaaagagagt gtgatagagt agctacaacc
     2761 tataaaaaat tacacaagca atatctatta ccttaaaagc atcccgccat atttttaagc
     2821 attttgtgaa gtaataaaat atttactgtg ttatgatagc atgtactata gagaaataga
     2881 ggtattaact tatggaaaag ttaaatcaag ttatgttata tgttgatgat gttgacaaag
     2941 cgaaagcatt ttggacagaa actttagaat ttgttgttgt aagtgaaaca ccgttagctg
     3001 aggactatgt tgcagttgaa gtatcaccta cgaaagatgc tgaaacgtca ttgacgatta
     3061 tggcaaaaga atttattgaa aagtatagcc cggaagtgaa cctaggaaca ccatctttaa
     3121 tgtttaaaga gaaaaacttt gatgcattat actcaaaatt aaatgattta ggtttaactg
     3181 ggcatgatat agtagaaatg aatggtcaac gtgtatttaa ttttcaagat ggccaaggta
     3241 actattttgc agttagtgac taaacaatcg attttaatat ttatacaaac ctatatattg
     3301 attttcggat tgaatttcag tatgaaggtt ttatttttat cgttttatgc aatcgtttgc
     3361 acaaatcttt tgactttttt agaaagtgtg actaaactgg tattatacag tggttggaag
     3421 cgttttccga tgcagaaatt aacaggaggt gattaaatgg ttacgattaa agatgttgca
     3481 ctaaaagccg gtgtttctcc ttcgacagtt tcaagagtta taaaaggaaa tcaacgtatt
     3541 agcgaagcga caatttcaaa agtgaagaaa gttatggaag aattgaatta ttttcctaat
     3601 accgctgcta gaactttaat tacaaaccaa acatataaaa ttggtttagt gttaaaaggg
     3661 tctgaggagc ctattcgact gaatccattc tacatcaatg tattgctagg aatttctgaa
     3721 acgtgtaacc agcatggcta tggtacacaa acgacagtct caaataatat gaatgattta
     3781 atggatgaag tttataaaat gattaaacaa cgaatggttg atgcgtttat actgctttat
     3841 tcaaaagaaa atgatccaat taaacaaatg ttaattgatg aaagcatgcc atttattgtg
     3901 attggtaagc ctacatcgga tatagatcat caatttacac acatagataa tgataatata
     3961 ttagcttctg aaaatttgac acgacatgtt attgaacaag gtgtagatga attaatattt
     4021 attacagaaa aaggaaattt tgaagtttca aaagatagaa ttcaaggatt tgaaacggtt
     4081 gcatcacaat ttaatctgga ttatcaaatt attgagacta gtaatgaaag aaaagttatt
     4141 tttaattaca tgcaaaatct acatacgcgc ttgaaggacc caaatattaa acaggcaatc
     4201 atttcgttag atgctatgtt acatttagcg attttaagtg tgctatatga acttaatatt
     4261 gaaattccaa aagatgtaat gacagcaaca ttcaatgatt cttatttaac cgaaattgcg
     4321 tcaccacctc aaaccagtat tgacatcaag cctcgaatgt taggtcaaca ggctggttca
     4381 gcaattttaa atatattgaa aaacaaagca caggatgtta ttgaactcgt cattatagat
     4441 acagaattaa aaataagaaa atcaacacag cgatagcaaa ggagttttaa aatgaataag
     4501 caatggtgga aagaagcagt agcatatcaa gtatatccaa gaagttttaa tgatagtaat
     4561 cacgatggta ttggggattt acctggaatg attgataaat tggactactt aaaagattta
     4621 ggtatcgatg tcatttggct cagtccaatg tttaaatcac ctaatgatga caatggttat
     4681 gatattagtg actatcaaga aattatggat gaatttggaa cgatggaaga ctttgattgt
     4741 ttattaaaag gtgttcatga tagaggcatg aagcttattt tagatttagt tgtaaatcat
     4801 acatctgatg aacatccttg gtttatagaa tccaaatcta gtaaagacaa tcccaaacgt
     4861 gattggtaca tttggcaaga tccaaagcca gatggctctg aacctaacaa ctgggaaagt
     4921 atatttaatg gatctacatg ggaatatgat gctaatactg agcaatatta tttccattta
     4981 ttcagtaaaa aacaacctga tttgaattgg ggtaatccgg aagttagaga tgctgtattt
     5041 gaaatgatga actggtggtt tgataaaggc attgatggat ttagagtaga tgcaattacg
     5101 catattaaga agacgtttga agcgggtgac ttacctgtac ctgagggtaa aacatatgcc
     5161 ccagcatttg atgtagatat gaatcagcca ggtatacaaa cttggttaca agagatgaaa
     5221 gatcgctcat taagtaagta tgacattatg actgttggtg aagcgaatgg tgtaagccct
     5281 gatgatgctg atgactgggt cggggaagaa aatggtaaat ttaatatgat attccaattt
     5341 gaacatttgg gactgtggaa tagtggtgat tctcactttg atgtaaattc gtataaatct
     5401 gtattaaata gatggcaaaa acaacttgaa aataaaggtt ggaatgcgtt gtttattgaa
     5461 aatcacgacc aaccacgacg tgtatcgacg tggggtgacg atgacaagta ttggtatgaa
     5521 tcagcaacaa gtcatgcagc tgtttatttc ttgcaacaag gtacgccatt catttatcaa
     5581 ggtcaagaaa ttggtatgac gaattatcca tttgaaagta ttgaaacgtt taacgatgtt
     5641 gctgttaaaa atgactatca aatagtgaaa gctcaaggtg gagatgtaga cgctttactt
     5701 gcgaaatata aagatgagaa ccgagataat tctcgcacac caatgcaatg ggatgatacg
     5761 ttaaatggag gatttacaaa tggtgaaccg tggttcccag tgaatccgaa ttataaaact
     5821 atcaatgttg cacaacaatt agaagatgag cattcagtat tacaatttta taaagattta
     5881 attcaattaa gaaagtctaa tgatgtatac gtatatggtc aatttgattt agtagatgct
     5941 gaaaattcac aagtttttgc gtacacgaga acattaaatg aaaagcaagt tcttattgta
     6001 ggtaatctta ctaaccacga agcagaatta actgtaccat ttgatttaag ccatggagaa
     6061 gtaaagctat ttaattatga tgccaaagtt aatttaaaac agttacgtcc atatgaagca
     6121 tgtgttatcg aactaaatta acatattaat atgcccccta atagaatcta aggccatttg
     6181 tagacctata gatatattag ggggcttctt tgtttttaat ttttaatctt cgtcataagg
     6241 actctgttta acaggaatcc aaagctgaat tttagtgaat gggtcattaa atgaaatatc
     6301 gagagggtac acttcaacat ataaatcatt tcgttcatat ggcaatgtta actgcaaact
     6361 tgattcaata tagtaccaag cttcattagt tgcataatca atttcacctt gtaaattgaa
     6421 tttcgcacaa tgtttgccag gtaaaaatcg gctttctaag tgtgcaggat aacgttcact
     6481 tggtacacct acaaatattt ctaaaccatt ttcaagagga caactaataa caaatagttc
     6541 aaatggacta acgtcattat atcgtcgtaa ctctttaatt ttaccatcaa tgagcaagtc
     6601 ttctaaaaaa tccggaacat taaaaggatg tgacaaatac ttagtgtcta taaatcgtgc
     6661 atatccaacc aatgaaatat catctgtctc ttctaatctg tatggatagg gtgttctctc
     6721 agtagttgat aatttgatat ataatcgctc ttgaatttgt aattcatctt ttttagtaga
     6781 agcttgaata ggtgatacgc cgtgaaaatc actaaaatca tttgcaaaat catttgaatt
     6841 tgcatagtga tattttttag cgatatctac aagtcgtgta gcaccattaa tcacatcatt
     6901 tgcagcgagt gtcattttac gcgcgcgtgc ataagcttct ggagataagc cgacaatcat
     6961 tttaaatgat tgatcaagat ggtatggcga aagaccaacg taatcactta attcttgcaa
     7021 attgaaaggc tctaataaac gatcttcaat ataaacaatt gcctgttgta tttgcttgat
     7081 aacgtccaaa actttcactc caattaattc aatcatttat ttttattcta cattatttct
     7141 ataaattata cacccatttg ttcaatgatt attaaaatag ttttgggcat tgtaaaatat
     7201 aatttcataa tatagtctag aaaaaaagcg aatgatagaa caattgattt acttgattcg
     7261 taatcaatcc ttgtcattcg ctcatttatt tttgtttaac atgtgcgttt taattcaatt
     7321 attgaatatc gtcccaccaa tggttaccat cacgagcaag tagtaaatca ctttctaatg
     7381 gaccattagt acctgattca tagttaggga attctggatc aaccatattc cattcatctt
     7441 gaattgcatc aacaaatttc catgttgatt ttaattcttc ccagtgcgtg aagttagtgg
     7501 catcaccttt aagacaatcg aataatagat tttcatatgc atctacagta ttcattttat
     7561 cttgagcgct cattgaataa gacaattgaa caggttcagt ttcgatacct tgtgtatttt
     7621 tcttagcatt tagatgtaaa gatacacctt cattaggttg gatattgatt actaataggt
     7681 ttgaatctaa tagtttatca gtttcatagt ataagttcat tggtacttct ttaaattcaa
     7741 cgacaacttg aattgtttta gatttcatac gtttaccagt acgaatatag aatggtacac
     7801 cagcccatct aaagttatca attgttaatt tacctgaaac aaaggtaggt gtgttagagt
     7861 catctgcaac gcgatcttca tcacggtatg ctttaacttg tttaccatcg atatagcctt
     7921 cgccatattg accacgaaca aagttctttt taacatcttc agattggaaa tgacgcagtg
     7981 atttaagtac ttttactttc tcagcacgga tatcttcact atttaaacta ataggtgctt
     8041 ccatagctaa taatgcaacc atttgtaaca tgtggttttg caccatatct tttagcgcgc
     8101 cacttgattc ataataacca ccacgatctt caacacctag tatttcagat gatgtaactt
     8161 ggatgtttga aatatatttg ttattccata atggttcaaa catcgcattc gcaaaacgta
     8221 atacctcgat attttgaacc atgtcttttc ctaaatagtg gtcaatacga taaatttctt
     8281 cttctttaaa tgatttacga atttgattgt ttaatgcttc ggctgatttt aaatcactac
     8341 cgaatggttt ttcgataaca aggcgtttaa atccttttgt atcagtaaga ccagaagatt
     8401 ttagataatc agaaataacg ccaaagaatt gtggtgccat tgctaaatag aatagtcgat
     8461 taccttttaa ttcaaattgg ctatctaatt cattactaaa atctagtaat tcttgatagc
     8521 tttcttcatt actaacatca tgtctatgat agaagacatg ttccataaac gcgtcaattt
     8581 tgtttgtatc ttttacgtgc ttttgaattg atgattttac ttgattacgg aaatcatcat
     8641 tagtaatgtc acgacgtcca ataccgatga tggcaatatg ttcatcaaaa ttgtcttgtt
     8701 ggtagagatg gaatattgat ggaaacaact tacgatggct taagtcacca gttgcaccaa
     8761 agattgtgat taaacaaggg atgtgtttgt ttttagtact caagattaaa acctcaattc
     8821 ttaattagat atatgattta ttattataag ataatccatt cagcaggtca ttcaatatgc
     8881 tcgacagtat gcagtgtctt aaatgaaata tcgattcatg tatcatgttt aatgtgatac
     8941 ttattaatga taagtataac gtaattatca aaatttatat agttttgtct acgttaaagt
     9001 tagaaaaatt aactagcaaa gacgaatttt taacagattt tgattcaagt ataaattaaa
     9061 actaaattga tacaaatttt atgataaaat gaattgaaga aaaggagggg catatatgga
     9121 agttacattt tttggaacga gtgcaggttt gcctacaaaa gagagaaata cacaagcaat
     9181 cgccttaaat ttagaaccat attccaattc catatggctt ttcgacgttg gtgaaggtac
     9241 acagcaccaa attttacatc atgcaattaa attaggaaaa gtgacacata tatttattac
     9301 tcatatgcat ggcgatcata tttttggttt gccaggatta ctttctagtc gttcttttca
     9361 gggcggtgaa cagaagccgc ttacattggt tggaccaaaa ggaattaaag catatgtgga
     9421 aatgtctatg aatttatcag agtcacattt aaactatcca attacatata ttgaaatcga
     9481 tgatcatctg acatatcatc atgatggttt tactgtagag gcgcatttac ttaatcatgg
     9541 tgttccatca tatggatata gagtaatggc acctgaaact acaggtacaa tcaatgttga
     9601 agcgttaaaa aacattggtc ttgaaccagg tcctaaatat caagaagtaa aatctcatga
     9661 tacatttgaa cataatggtc aagtttatca atcaaaagat tttagaggtg agtccaaaca
     9721 gggtccagtt gtagcaattt ttggtgatac aaaaccatgt tctaatgaac gtgtgatttc
     9781 tagagatgct gatgtgatgg tacatgaagc gacctatata gatggtgaaa agcatttagc
     9841 caacaattat catcatagcc atattgaaga cgtatttgca ttgataaaag aagccaatgt
     9901 taaacgtacg ttgattacac atttgagcaa tcgctataat acagaagata taaatgaaat
     9961 ttatcaaaca ttgatacaaa atgaagatac acctaatttt aattttgtaa aagattttga
    10021 tagttttaaa gtctaatgac aaagtaataa acaatgcttg ccaaatagta ataatttata
    10081 aaaataaccc gtccaactat gtatgcgatg atgcatacat gtgttggcgg gtttgttttt
    10141 attggtcttc tacattagaa agttcaatac tacggtcgac ggcagcattt agacaatctt
    10201 cgaaaataga tactaaatca tattgtgaca atgtatcaag gccagcttgt gtcgtaccac
    10261 ctttagaggt aatatttttt cttaattgag ccatgctcaa atctgaacgt tcaatcatct
    10321 tacttgtacc tataataagg ttgcgtatag attcttcaac ttgttctttt tctagaccaa
    10381 gtttcgtacc agctttaacg tattgctcga atacatgata taaaaatgct gggccgcttc
    10441 cggtgatagc tgttacttga tgtaaatgat cttctgatac ttcaattaca gaaccaaatg
    10501 ctttaactaa atcgttaatt tcatctttag atttagggtc aaagttgttt gaaaaactaa
    10561 tgccagtaac agagtgtccg acttgcgcat ttgtgtttgg cataattcta gcaactggat
    10621 tttggcattc taattgttgt ttaatataat caatcggaat acctgccata attgaaatga
    10681 agcaattgtc ttttgtgata tgtggtttga tgcgtgttgc tagagcatca aagtcatgtg
    10741 gtttggtacc taaaaaaaca taatctgcat cttttaataa tgtcgcatca tcataactat
    10801 agttaacacc tagtttttca gcgaatgctt ttaaagcttg ttcattagat ttatttgtta
    10861 aatatatatc attggcatct aagttgcttg agttaataat tcctgtaaat atagcttgtg
    10921 ccatattacc agctccataa aatacgagtt tcattaacgt cacatccctt tagtttttga
    10981 tttgtatgtt atattttctt attattgtat atatattagg aaagataatc aacttgtctc
    11041 aattatgtaa agttgataaa ttgcattcta tataattgaa aaggaaggtt aattgatgaa
    11101 aggacaacat tttatagtga ctggtggtac gagtggttta ggtttatcga ttgtccgaaa
    11161 gttactagaa aataaagtgc atgtaacgtt gttggtgcgt gatgttgata aagcaactag
    11221 aatctttgaa caggaattgg agaaaactat aaatgttatt ccttgtgact taaatgattt
    11281 gaagtcgata caggcactgc aatttgaaga caatacatca ttcgatggat tcatttatag
    11341 tgcaggttta ggttatttca aatctatttc agaccatagt ttttcagaaa tgattgaaac
    11401 gtatcagctt aatttaatta gttttaatgt tttatatact gtgttgagac catatttaac
    11461 atctaacgca catattgtag gtatttcaag tcaagctgca ttttccacac aagcaaatgc
    11521 tgcacattat ggggcatcga aagcagggtt ttatgcatta atgaatgctt taagactaga
    11581 gtcaccaaac ttacatatca tgacagttaa tgttggcccg attgatacac cttttcatca
    11641 aaaagccgac ccatcaatga agtatgctaa aaggatgggg aaaattatgc ttgatgctaa
    11701 tcagcttgca gaagatatca tttatggcat taaaacaaaa caattggaaa ttaatcggcc
    11761 taaatggatg catcatgcgt tgaaaatgta tcaaattgcg cctagatttt ttgaacgatg
    11821 tttccctaaa ttatttaaaa ataaagcata aataggtggg atggcaatga actgtcaatc
    11881 ggagagtgtg gtaagatttt gtgtacgata tgcggaacaa ctatcagtgt ttgaagaatt
    11941 tacagtgctg gatattttag gcgatatttc tgtagaccaa atttcagaca gtacattata
    12001 ttatacttgt gaaaaattca aactacttgt gttgcaagga aatgtgctgg gtgtacaaat
    12061 aataactaat aatgatgaat caacatgcga agtgaaatat agaaagatgt tttaataaaa
    12121 ttattaagag atgaagtggc agaagtttgc ttaaggcaat gccgcaaggc gaaattacta
    12181 aatactgatg cgtgaggaca acgctttaat ttctatcata agtgagtaat cataatcata
    12241 aattatagcg aattgtacat aaacaacagc agtaaggcat ttttataatg aaacgcctca
    12301 tgctgctgtt tttattactg aaaatgactt ctacactaaa tgattggtat attccaaatc
    12361 ctttacacga gctcttgctg ttttaatctg atctaaacta actttagtat gatagttttc
    12421 aatattttct tttaattggt cgacgctact tgcacctaca atgatggaac caagcacgtc
    12481 atgtgatgtt aaataactaa atgtcaatgc agataaatta ctttcaattt ctttaataga
    12541 ggctattgtt tcacccaatt catcatgaga ataatcaaaa ataccatctt taaatttatt
    12601 gtcgagcaca ttaacactgt ttgaagttaa taatccttta gacacaggtc ctcttgccaa
    12661 tactttaaca tcattgttgt gaattgcatc taataatgat tctggacgat tatcaatcaa
    12721 attgaattga gacattatcg tttcgatttg actatgtttt aaataataat caattacatt
    12781 tgggcgaata gaagaaatac cgtaagcacg tataattcct tcttgtttca attcatcaaa
    12841 tgcgcttatt gtttcgtcta atgggtcatc aatggttccg ccatgaagtt gatataaatc
    12901 gatatgatcg atacctaaac gctttagtga acctttaact gcctctttaa tataggattt
    12961 actcggatcc cattttgtac tgccatcttt tgttaaacga ttgcctactt ttgtaccaat
    13021 gacaatatca tcgcgttgtt gatatttaag aagtgcctta ccaacaactg attcatttat
    13081 acctttatcg tacatatctg ctgtatcaaa ataagtgata ccattttcaa cagcacaatc
    13141 tataatttgt tcggcttttt ttaaatctgt acctaaactc atgcaaccta gacctaattc
    13201 agataatgaa ataccacttt ttaatatatt tttttgcatg tctgttctcc tttcgataca
    13261 ctttagctaa caagcatagt gtatcaatat ataatatgaa tttaaaggaa cgtgattata
    13321 atggatttaa atgaaaaaac aattgatcga acagttattt ataatggcaa aattgtagat
    13381 gtagaaattc atacagtgac attaccaaac ggtgaaacat caacaagaga attagtttat
    13441 cataatggtg cagttgctgt ttgtgcagtg acacctaaaa aagaagtcgt attagtgaaa
    13501 cagtatcgta aaccagtaga aaaaccattg ctagaaattc cagctggtaa attagaagat
    13561 gatgaagata gagtcgaagc ggcaaagcgt gaattagaag aagaaacagg atatattgcc
    13621 aaagagttaa cacatgttgt agatatgtat ggttcaccag gtttttgcga tgaacaatta
    13681 tcaatatatt ttacggataa tttagaagaa ggtacagttc acttagatga agatgaattt
    13741 gtcgaagtca ttaaagttcc gattgagaat gttaaatcga tgctaatgaa taaagaaata
    13801 gaagatgcga agactatcat tgcattgcaa cacctattat taaattataa tcattctaaa
    13861 taacaaatga gaaaagcttg cattttattg agaaaactgt tagttttaat tgtgaagttt
    13921 gaaataattt gtaatgattt taattattag taggggagtg gacatcgttg gaagaacgat
    13981 taaatcgcgt taagcaacaa ttacaacaat catcatataa gctaacgcca caacgcgaag
    14041 ctactgttag agttctaatt gaaaatgaaa aagatcatct aagtgctgaa gacgtatatc
    14101 tgaaagtaaa agataaagcg cctgaaattg gcttggcgac agtatacaga acgttagagt
    14161 tgttagctga actaaaagtt gtcgacaaaa ttaactttgg tgatggcgtc gctcgttttg
    14221 atttaagaaa agaaggcgca aaacatttcc accatcattt agtatgtatg gaatgtggtc
    14281 gtgtagatga aatcgatgaa gatttgttac cagaagttga aaatcgagtt gaaaatgagt
    14341 tcaattttaa aattttagat catcgtttaa ctttccatgg tgtgtgtgaa acgtgccaag
    14401 ctaaaggtaa aggatagtaa attgcgtagg ttaaattaac cttcgctttt tttagaggtg
    14461 tggttatgga aacaattatt gaagaatatt tgcgttttat acaaattgaa aaaggactaa
    14521 gttctaacac aattggtgct tatagacgtg atttgaaaaa gtatcaagat tatatgactg
    14581 aacatcatat ctcgcatatt gattttatag atcgacaatt aattcaagag tgtttggggc
    14641 atttaataga ccaagggcaa tctgctaaat ctattgcgcg atttatttca acaatccgta
    14701 gttttcatca atttgctata agagaaaaat atgcggcgaa agatccaacg gtattattag
    14761 attcaccaaa atatgacaaa aaattgcctg acgttttaaa tgttgacgaa gtgttggctt
    14821 tattagaaac gccggatcta aataaaatta atggttatcg tgatcgtacg atgttagaac
    14881 ttctgtacgc aacgggaatg cgtgtatctg aattgataca tttagagtta gaaaacgtga
    14941 acttaataat gggatttgta cgcgtatttg gtaaaggcga taaagaaaga attgtaccat
    15001 taggcgacgc agtcattgag tacttaacta cttatattga aacgattaga ccgcaacttt
    15061 taaaaaagac tgttaccgaa gtcttatttt taaatatgca tggtaaacct ttatcacgac
    15121 aagcaatatg gaaaatgatt aaacaaaatg gtgtaaaggc aaacattaaa aagacgttaa
    15181 cgccacatac gttacgccac tcttttgcga cacatttatt ggaaaatggc gcagatttaa
    15241 gagcagtgca agagatgtta ggtcactctg acatatctac tacgcaactc tatacacatg
    15301 tttcaaaatc tcaaattaga aaaatgtata accaatttca tcctagagca taaagtgaac
    15361 cataactcaa aagtcacaat acacatgact aaaaatgtct gtgctattgt ggctttttta
    15421 aattggttga ttaattacgt ctatgttttc ttaattgaat cgcttcttct tttgctgaaa
    15481 tcacttctga acgatcacgc cgcatgtgat ggtctacaat aaaaggatct gttgatgttt
    15541 cctgattata atcatagtct ggatagttgg ccttgatgat gcgttcaaag tctggagtta
    15601 ttggtaatat aacagatgaa aaaggctttg ctgcattcaa ttttgcaatc tgttgctcaa
    15661 ttaacaattg ataatcattt aaattaaggt ataacgcatc tctatcttta gcattttgta
    15721 ttatttcttt agatttatta aaagacttat aggcgccttt taaattattg cggcgataat
    15781 ggtaacaagc agttgcaaac aagattaaac taacaactgc atcttgctta ctgtagttat
    15841 tttcagcttt ccatgcatct tctaaaatgt catgacatag gaaataatgt tgcttagtat
    15901 gaaattgata atagaaattt atcagtgcct gttgcatttt gttatcaccc caatttaaaa
    15961 gtaagttatt ttcatgctat aatattttag agaattatgc acatatgacg caatacgagg
    16021 tagatattat gtatgaagtt aaattagatg ctttcaatgg accattagat ttattactgc
    16081 atcttatcca aaaatttgaa atagatattt atgatattcc tatgcaagca ttaacagagc
    16141 agtatatgca gtacgttcat gcaatgaaac agcttgaaat taatattgca agtgaatacc
    16201 tagtattagc gtcagaactc ttaatgatta aaagtaagat gctattacca caatcaacat
    16261 cagatatgga tgttgatgat gacccacggg aagatttagt tgggcgttta atagaatatc
    16321 aaaattataa agaatatact gctattttaa atgacatgaa agaagaaaga gatttttatt
    16381 ttactaaaag accgacagat ttatctcatt tggaaacgga tgaatcttgg gatccaaatc
    16441 atacgattga tttaactgaa ttaattgtag cttatcaaag agttaaaaat agagttgagt
    16501 taaatacacc taaatctgtt gaaatccgaa aagagacatt taccattcaa caagctacag
    16561 aacaagtgac atcgagattg aaagataaag atcattttaa cttctttagt ctgtttacgt
    16621 tttctgagcc aattgaacaa gtagtcactc acattttagc tattttagag atgtcaaaag
    16681 caggaataat taatattgag caacaacgta attttgaaga tattaacatt attagaggag
    16741 tgaactacca ttttggataa tcatggtata ttagagtcgc tattatttac agctggcgat
    16801 gaaggtttag atgaaaaaca actattagaa atattagata tgtcgaaaaa tcaactcgtt
    16861 gaattaattg aaaattattc atcacatgga ttaatgatac aacgatttgg aacgacgtat
    16921 gttttaacga ctaaaaaaga agcggcaacg tatattgaac aattaattga acaaaagtca
    16981 caaatgaaat tatcacaagc agcaatggaa gttctatcaa ttattgctta taaccagcca
    17041 ttatcaagaa gtgatattga attaattcgt agtatcaatt cagatggtgc ggtcaagaca
    17101 ttgattgcca aaggactagt tgaggctaaa gtggtcaatg aacagcgtag ccaacagtta
    17161 attactactg atttattttt aaatgtattt ggtatttcca atatagaaga tttgccgaca
    17221 actgaagaag acgatgaaga aatggatgct ttcttcagta atctagtcaa tcaaaaagga
    17281 gaaaataatg actaaagaat tagaacgatt acaaaaacgt atagctaata gcggttatac
    17341 ttcaagacgt aaggcagaaa ctttaatttc tgaaggaaaa gtgaaagtga atggtacgac
    17401 tgtcactgaa ttaggaacaa aagtaaagcc atcagatacg attgaagttg aaggtattaa
    17461 aatagaacta gaagacaaaa tttacatttt attccataaa ccaactcaag tgataacaag
    17521 tgtatcagat gatagaggac gtacagtggt tacggattac tttaaagata tagaagcacg
    17581 tatttatcct gttggtcgtt tagactatga tacttctgga ttgttattac ttacaaatga
    17641 tggtgaattt actaatttaa tgacacatcc aagatatcaa attaagaaga aatatgtagc
    17701 aaaattaaaa ggttacttaa tgagagaaga agtgaaagcc ctagaaaaag gtattgaatt
    17761 agaagatggt atgacgcaac cggctgaagt gaaagttaag aaacaagata aagataaaaa
    17821 cacaacattg gttgaaatta caattacaga aggtcgtaat cgccaagtca gaagaatgtt
    17881 cgaacatttt ggtcatcaag tgacgaaact gtctcgtatt gaatatgggc ctttaaatgt
    17941 tgtcggtttg aatgctggtg aaggacgtgt attgacgcct catgaagtaa aagtgatgcg
    18001 tcatttagca gaacatggga aataattaaa taaaatatgt atttatcaca aagtttgaga
    18061 attttttcac aaaattttag gtttttaata actgtatgcg ctttcctgtg atataatact
    18121 tttgtaaaga aaagcatgtg tgggaggtat gacctgtatg tcgaacgaaa tacttatcgt
    18181 agatgatgag gatagaatca gaagattact taaaatgtat ttagaaagag aatcttttga
    18241 aatccatgaa gcaagtaatg gccaagaggc ttatgaactt gcaatggaga ataattatgc
    18301 ttgcatacta ctagatttaa tgttgcctga aatggatggt atccaggtgg caactaaatt
    18361 gcgtgaacat aaacaaacac cgattattat gttgactgct aaaggtgaag aaacaaaccg
    18421 tgttgaaggt tttgaatctg gtgcagatga ttatatcgtc aaaccatttt caccaagaga
    18481 agtagtctta agagttaaag cacttctaag aagaacgcaa tctacaactt tagaacaaag
    18541 cgaacctcac gcacgtgatg tgattgaatt taaacattta gaaatagata atgatgcaca
    18601 tcgcgtactt gctgataatc aagaagttaa tttgactcct aaagagtacg aattattaat
    18661 atatttagct aaaacaccaa ataaagtatt tgaccgtgaa caattattaa aagaagtttg
    18721 gcattatgaa ttctatggtg atttaagaac agttgatact catgttaaac gacttagaga
    18781 aaagttaaat cgtgtgtcta gtgaagctgc gcatatgatt caaacagtct ggggcgttgg
    18841 gtataaattt gaggttaaat ctaatgatga gccggctaaa tagtgtcgta attaaactgt
    18901 ggttaactat tattttaata gtgacgacag ttttaatttt attaagtatt gctttaatta
    18961 cctttatgca atactatttc acacaagaaa ccgaaaatgc cataagagaa gatgctagac
    19021 gtataagttc actggtcgaa caatcacata ataaagaaga agcaataaaa tatagtcaaa
    19081 cattaattga aaatcctggt gggttgatga ttataaataa taaacatcgt caatcaacgg
    19141 cttcactttc taatattaaa aagcaaatgt tgaatgaagt agtcaacaac gaccattttg
    19201 acgatgtgtt tgataaaggt aaatctgtta ctcgaaatgt aacgattaaa gaaaagggct
    19261 catctcaaac atatattttg ttaggctatc caacaaaagc acagaagaat agtcatagca
    19321 aatatagtgg agtctttata tataaagact tgaaatcaat cgaagataca aataatgcta
    19381 ttacgattat cactataatt acggctgtta ttttcttaac aattacaaca gtctttgcgt
    19441 ttttcttatc atcaagaatt acaaaacctt taagacgttt aagagaccaa gctacacgtg
    19501 tatctgaagg ggattactct tataaacctt ctgtcacaac gaaagatgaa attggtcaat
    19561 tatcgcaggc atttaatcag atgagtacag aaatcgaaga gcatgtcgac gcattatcca
    19621 catctaaaaa tattagagac agcttaatta actctatggt agaaggtgtc ctaggtatta
    19681 atgagagtcg acaaattatc ttatctaata agatggcgaa tgatattatg gacaatattg
    19741 atgaagatgc taaagctttc ttattaagac aaattgaaga tacttttaaa tcaaaacaaa
    19801 cggaaatgcg tgatttagaa atgaatgcac gattctttgt tgtgaccaca agctatatcg
    19861 acaagattga acagggaggt aaaagtggtg ttgttgtgac agttcgtgat atgactaatg
    19921 agcacaatct agatcaaatg aagaaagatt tcattgctaa tgtatcacat gaattacgta
    19981 caccgatatc attacttcaa ggttatactg aatcaattgt agatggtatt gttacagaac
    20041 cggatgaaat aaaagaatcg cttgccattg tccttgatga atcgaaacgt ttaaatcgtt
    20101 tagttaatga attgttaaat gtcgcacgca tggatgctga agggttatcc gtaaataaag
    20161 aagttcagcc tattgcagcg ttactagata agatgaaaat taagtatcgc caacaagctg
    20221 atgatttagg tctaaatatg acttttaatt attgtaagaa gcgtgtttgg agttatgata
    20281 tggatcgcat ggaccaagta ctaacgaact taattgataa tgcatcacgt tatacgaaac
    20341 ctggagatga aattgcaatt acttgtgatg aaaatgaaag cgaagatatt ttatacatta
    20401 aagatacagg tacaggcatt gcaccagaac atttacaaca agtatttgat cgtttttata
    20461 aagttgatgc agcgagaacg cgaggtaaac aaggtaccgg tttaggtttg ttcatttgta
    20521 aaatgattat cgaagagcat ggtggttcca tagatgttaa aagcgaatta gggaaaggca
    20581 caacatttat tattaaacta ccaaaaccag aataaaattg aatatagtta tttcagaacg
    20641 catgttattg attagagact ctaatttata gcatgcgttt tttgattgat
//
LOCUS       DBNENW010000037        20560 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNENW010000037 DBNENW010000000
VERSION     DBNENW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.037"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(113..673)
                     /locus_tag="AC1LW8_001059"
     CDS             complement(113..673)
                     /locus_tag="AC1LW8_001059"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HIP4772909.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(842..2749)
                     /gene="sasF"
                     /locus_tag="AC1LW8_001060"
     CDS             complement(842..2749)
                     /gene="sasF"
                     /locus_tag="AC1LW8_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HIP4772910.1"
                     /translation="MAKYRGEPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKYASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(2991..4349)
                     /gene="gtfB"
                     /locus_tag="AC1LW8_001061"
     CDS             complement(2991..4349)
                     /gene="gtfB"
                     /locus_tag="AC1LW8_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HIP4772911.1"
                     /translation="MIKLFDYFNNHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFQDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(4342..5850)
                     /gene="gtfA"
                     /locus_tag="AC1LW8_001062"
     CDS             complement(4342..5850)
                     /gene="gtfA"
                     /locus_tag="AC1LW8_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HIP4772912.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(5868..8258)
                     /gene="secA2"
                     /locus_tag="AC1LW8_001063"
     CDS             complement(5868..8258)
                     /gene="secA2"
                     /locus_tag="AC1LW8_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HIP4772913.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWGDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNLYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(8248..9207)
                     /gene="asp3"
                     /locus_tag="AC1LW8_001064"
     CDS             complement(8248..9207)
                     /gene="asp3"
                     /locus_tag="AC1LW8_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HIP4772914.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLVFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDKINVIIAEPSDVYPTISNDFLKLFGHVWLVERWMEDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLKEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(9185..10753)
                     /gene="asp2"
                     /locus_tag="AC1LW8_001065"
     CDS             complement(9185..10753)
                     /gene="asp2"
                     /locus_tag="AC1LW8_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HIP4772915.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPNAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(10743..12296)
                     /gene="asp1"
                     /locus_tag="AC1LW8_001066"
     CDS             complement(10743..12296)
                     /gene="asp1"
                     /locus_tag="AC1LW8_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HIP4772916.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLTETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(12307..13518)
                     /gene="secY2"
                     /locus_tag="AC1LW8_001067"
     CDS             complement(12307..13518)
                     /gene="secY2"
                     /locus_tag="AC1LW8_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HIP4772917.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTIIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLSQYW"
     gene            complement(13648..20475)
                     /gene="sasA"
                     /locus_tag="AC1LW8_001068"
                     /gene_synonym="sraP"
     CDS             complement(13648..20475)
                     /gene="sasA"
                     /locus_tag="AC1LW8_001068"
                     /gene_synonym="sraP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HIP4772918.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLKVQPTNNTFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSTSESSSTSASLSDS
                     TSNAISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSLSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQSMSGSTYNSTSVSDSESVSTS
                     TSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHT
                     HSTSTSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGG
                     VMTLLVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         6002 a         3924 c         3275 g         7359 t
ORIGIN      
        1 caaatgttcc tcatcatatc cgtttcaatt gccatctcac acattttata aatatgagca
       61 aatgtactta ttctcaaaca ttactgccga gctttaattg acgttatatt aactataaac
      121 tacttttcca tgactctacg gattcaatgt cacatgagcg tgataaaatt tgttcaataa
      181 taaagtcatg tttatcatct gatctatcac caacagcatc ttctaaaaca gtaatataat
      241 agtctttatc tacactttct aatgccgtgc tcaatacagc tccacttgta gagacacccg
      301 ttaatactaa atgattaata tcatttgcac gtaaataaac ttccaagtaa ctacctgtaa
      361 atgcgctaaa gcgtcgctta gaaataatcg gctcatcttc tagtggtgct aaatcttcaa
      421 gtattcgtgt agatgcatct gcttcagtaa tcgcatatcc ttgagcttta attgttgaaa
      481 acactttatt actcgaggag acatcattaa aatgcttatc taacactaaa cgtatgaaaa
      541 tgactggtat tcgatgttgt cttgctgctt caattgctct ctgattcgct ttaataatat
      601 tttttattct aggtacacta ctcgctatac cttcttgcat atccaaaact aatagcgccg
      661 tttttcgaga catcttcatt ctcctttact tgtgtagttc taagtcgtta aattcattat
      721 aacgttaaaa tgatggacaa tctattcatt gcattttgca tatacttcac aataatttaa
      781 ggggaaataa gacgtcttat atacttaaaa aaaatatata gatgctcttc ccccaatata
      841 attatgcttt atttttcaac ttattgcgtc gtgataacca aatcattagt acacccattg
      901 caccaacaat tacagatatc ggcaaccaat gttcttttat cgtttccccc gctttaggca
      961 aagatacatt accatcagca tttaataatc cacttaacaa tccattacct ttaccaagtg
     1021 ttaacgtctt ttctggcttt ggtgtgggta tatctggaat actgtctaat aaatttgatc
     1081 cttgattcat taaatttgct aacttattta aatccgtcgt tttcccattt ttattcaatc
     1141 gatctagtaa acttggacga tttactattg gtgataaaat atagtctata tcttttttcg
     1201 tttgattgag tctcttttgt aaattcaata aatcatccgc tttaccattc aatgccgatt
     1261 taactaaatt aaaaatttta ttttgatctg tttctatttt agtaattaaa tctgccagta
     1321 attttgcctt ttgtctttct atacgtgttg ctaaaatcgt ttcaattgct tgttttttat
     1381 ctttcgcatt attcaaaatt gcttttaata tatcatctga agacgtgtcg ccagttgatg
     1441 caaaatgttt cttcaattgg tcaacgattt ggcgatttga taatccttta ttcgtccaat
     1501 ctttagccaa tttatctgct tcagcttttc ctaatttcgt ttgtaaaatt tgagaaatca
     1561 atagcgactt atcttgtgat tgatcaatca atgacgttaa taaatcatca ctcgttgtca
     1621 gagatagttg atcaatatga cgagtaattt gatctgcaat ttgttgatcc gttttaccat
     1681 caacacgtat atcttttaga attttatctg cctcgtcttt attaaatata ctttctaaaa
     1741 tgctttgtgt agcatacttt ttatcatcag tacgtgcaag ttcttccaaa ataatatttc
     1801 gttgactttt tatacgctct ttcgtcttat ttacttcgct cattaagtct gatttttgat
     1861 ttttaggaag ttgcgtattt gcaatacgtt gatctaaaga ttgtaacgta ttcagtttat
     1921 gatatgtgta atgttgcgtt gaggcattac ttttagccaa tttttcaatc atagcatgat
     1981 taattttatc gcttccttgt aatttatcag tgagtcgatt actatggctt tgattctctt
     2041 catttgaaag gaatttattt aacacaacat gtccagaacc atcattattt ggcgttttag
     2101 ctacttcatg attactatct gttgtagaca ctgctggatc tttcgatgca tatttcaatg
     2161 catctttcga tttgtgtatt tgctgattca aatggtctag gtcttctaac gccttattta
     2221 ccattgcttc atcattttta tcatcttttt ctccatgttt tgttgtagcc gtttgtgaca
     2281 tatcattttt cattgcatta agatcgtcct cgccactttg ttgaccccta tcaacatttg
     2341 aagaaacctc atttaaatct ttaagcaatt gatctaattt actgtctata tcactttgac
     2401 cgttcatttc agtgtgagaa cttttattgt ctttgctatc caactcatta gctcgtttta
     2461 tgatttcatc tatttgcgat gctgttttcg cttcatttag ttgtgcttta taatgtgctt
     2521 tagatgaagc cgataactgt tttaattgct caatttgacg aattgctttg tcaactttgt
     2581 ctaataaatc ttgcttagat aatatctctt ttgaaatttc agtatccttt tcagatgcag
     2641 cttgggcatc gtacggcaag atattcgtta aaatgatact tgtcgccatc attgtcgaac
     2701 acgataactt tacatataat tgaaacggtt cccctcgata tttagccatc aatatactcc
     2761 ttcctcactt acttccttca aagaattaca tactattata tacctgttca caagaaattt
     2821 atacttatct atctagttat tgttgttagt aattatctac ttattactta gcttatattt
     2881 aagtaaacaa aacaagcatg acgtaatatc atattgtcca tgtcgcttac atcatattac
     2941 gtcaaatctt ttatattaaa tgatgcttta ttttagactg ctttttcttt ttagctttcg
     3001 agcgcctgtt taaaaacttg ctcgaattgt tcacgcgaga tttcgtgtgc atgtgctttt
     3061 tgtgctaata aagcatcttg aaactgttgt tgatctttca aactctctaa catttgtatt
     3121 aattggtctt tactttccat tgttatctca tcattatgct caaataagtg ctctgataat
     3181 gttactttag catggtgtgc ggtttgacga taacctaaaa tcaacaactc atagtcaaac
     3241 gcttgttcca ccgcatttaa aatttcatta ccctcattga tatcaagata aatatcacat
     3301 aactggtata gttcatttac cctatctata ttaatagatg ggtataaatg cacattagca
     3361 tattgatcaa gttgcattag cttatcagac atctctgtaa tagcagcgat gtgaaactta
     3421 aaatctggta aagtttcaac caataccttg atgttacgaa gttgatccga gttagttaat
     3481 attacaattt ctttagtata tctattacga ctacgatagt tatatagata tccgccttgt
     3541 aaaatacgag attgaacctt tgcgtctgct atattgagca tcgtttcata ttcgttttta
     3601 tctggaataa taatattaca atgtcgtttc atatcacctt tacacatcaa ttgcatattt
     3661 cccgggacat taccattaca gtgttcttgc cataccaaaa catcactacc ttttgatggc
     3721 aaattatata acactgaaaa tggtagggct agtgagttaa taacgaaatg atgttccgta
     3781 atttcaagtt gcttgataaa aaataatacg aatgcgagct ttgaagggaa aaagtaagac
     3841 ttcccttgcc aatccaatat gacatcagat gttacaaaat tttcataaat cacttcttta
     3901 ccttctgctg tcatatattt cttcaagatc gctttacgat ttaaatcgta aacagtttgt
     3961 gcaaatttaa taccattctt agaataataa tcgacaaatc ggacacgttg ttggtcatca
     4021 aaccattcga cacgactaac aattctaggg cgctctccac tttgataaaa tattttgcct
     4081 cgtagacgtc ccatatcatt aattgtagcc gaattgttgt tacctttaat ttcccaaaaa
     4141 gctggtacag taacctgatt aaaaaatcgt ggtttcatat tttctgtatt atgattatct
     4201 gcaaaaaatt gatacggtga tataacatcg tccggtaaaa agccattgtc attgagtaca
     4261 attgttaaat cttcttccaa cttactggct ttaaaagact catataactt tcgtgaatga
     4321 ttgttaaagt aatcaaataa tttaatcatg tagcacctct tgaactaatg tttcccattt
     4381 taaaataata tcttgagtca taaattgctg tgccacttca taagagatgt catgtggtgt
     4441 ctggggacca ttgttaaaat acattacaat ggcatgagct agttttgcga taacatcatc
     4501 cacactatct tcatcggtat caaaaggtac caagtagcca ttttccccat ctcgaataaa
     4561 ggttgggtta ccataattca catttaatcc aatcatacct agtcctgagc ctaccgcttc
     4621 cattagtgtt aacccaaaac cttcgctagt tgatgcagaa agaaataact cataatcatt
     4681 ataaatttca tcaagtttaa catgccctag taaacgaata taatcttgcg cgcggtgtgt
     4741 atcaataatt ttacgcagtc gcgtcttctc gctaccttct ccataaatat caaatgttaa
     4801 ttctggcact tgtcgtttag ccacgataac cgctttgaca agccaatcaa tatgtttctc
     4861 atttgctaaa cgagatgcac taatcatcgc atatggcttt cttgataatt taggatatga
     4921 taatgcatca atgcttccca caggaatagt ataaacacgt gggcgataac cttgatattg
     4981 ctcaaattgt cgacaaacca tatgattttg aatatctgtt gctgtaataa agaaatcaat
     5041 gtatttagct tttgaaaatt gatattcata ataattgttc catagtatat gctgctcact
     5101 catcatatta ttactataat gatcagcatg aatcacaaca ccaactttac tatcaccttt
     5161 atgctgcaaa acagcctgac caatatcaga agcgcggtct aatatgacaa tatcgtctcg
     5221 ggttaaattc aatcgttgta aaaagtatgc aataaattcg gttttgttat acaacaccgc
     5281 atcttcaaac acatatatag agctgtctcc atcaatatat tcgttataag cgatggaacc
     5341 atcttcatta tagaattgtc gcatatataa tttcgcttta ttatcagctg gtgcataata
     5401 ctcagaaaat atacgcgtat aactataaaa atctttacgt actaacatac tattaattac
     5461 aaattctgca cgatccacaa catctttttg ttcattttgt agataacatg ttacaaatga
     5521 tgatttccca ttaaaatata gacggactat cttaccattt ctttctctaa aactaatttc
     5581 atgaccaagc tcacgttcaa tgtcatctaa cgtgtacgtt gttggtgcta tagaaatatc
     5641 actaaaatac tgatacaacc aaataacttc ttgatcttta aacccaatgt tttgcgttaa
     5701 tgtctgtatg ttctctgact gtataaaatc taaaaacaca aatttagtgt cttgatttgt
     5761 acgtctcaat aatttagcac ggtaagcttg tgcatattca acaccgctac tcgcccagcc
     5821 tataccaaag tttatattat atattgtcat gcgctacccc ttttcattta tggaaaatgt
     5881 ataactggca taccctcttt atcaaatgta atcatgcttt gacaaatatt tttcaccatt
     5941 ctttttttga tatttcgtgt cataacttca aatgaatcta atgcaactct atggtattca
     6001 aaaatagcgt tacgttgccc attttgtcgt tgattaacgc ttgcttttaa ttgttgtaaa
     6061 tagtcgactt gttctaacca acatgaatca attgctttca aaaagacttt ttgaacgaaa
     6121 atattataat aatatgcact ttgcatgttt ttacgattca aagctaattg cttttcaaat
     6181 tgctctaata aaaatgtcac tactgcttgc ttatctttaa aattaacaca agccacatct
     6241 ttattaaatt ggaaacttaa attttgataa atatactcga caacacgcga ttttgttagc
     6301 accttttcct catttacaaa catttcaaat acatctttag ctaacgcttt aaaatcttga
     6361 ttctcagcat catctatttc taaaactcga ttgcgttcct cgtatacaag atctcgctgt
     6421 atactaatgc ttttttcaaa ttcattagcc atttcacgag ctttaacccc ttgttcttcc
     6481 gagatacgct gcgctttaac tacaatttgc ttaactttgc gattaaacaa attactttgc
     6541 gataatcgtt gtgcatctaa tgaatataat tgattatttt ccgctaaatt actatcgctc
     6601 catcgcttaa ctaaataatc atctagtgaa atatatatac aagatgatcc cggatcccct
     6661 tgtctaccag aacgaccacg taattgcctg tctacacggc tattttccat atgttcatga
     6721 ataataacag ctaatccacc taatgcttcg acaccttcac caagtttaat atctgtgcct
     6781 cgacctgcca tactagtcgc aacagtcatg gaaccaattt gccctgcttc agctatcatc
     6841 tgcgcttctt ttgcaacatt ttgcgcaatg agtaaattat taggaatatc catttggaat
     6901 aatactttcg aaaagtattc agccgcttca gcagttctcg ttatgagtaa aaccggtcgc
     6961 cccgtttcat gaagttcaac tatatcatga atcatcgcga tgtttttctc atcaactgaa
     7021 cgaaacactt tatctggttc atcgatacgt tgaatcgctt tatcagttgg tacttgtacg
     7081 actatttttg aatacaaatc aaagaactct gattcgccta attttcctgt agctgtcata
     7141 cctgaaaatg attcaaaaag tttaaataaa ttctggaagg taattgttgc cataacactt
     7201 ttatctgttg aaacctccat accttctttc gcttcaatag cttggtgaag tccagcttgc
     7261 aacttagttc ccggtaacat acgacctgta atacggtcaa ttaaaacaat atcaccatta
     7321 tatacaaagt aatcgacatt agattcaaac aaatattgcg cgcgcagtgc tagattaata
     7381 ttacgcacta ggaccatcgc ttgttcgcta tataaatctt caacattaaa gtatgattgt
     7441 gccgcttcaa taccttgatt taacagccat atttcttttt tggtcttctt cattttaaaa
     7501 tgcacgtctt caatcaatgt atctacaaac tctttcacaa tatgaaatag atttgattgt
     7561 aatcttggtg cgcccgaaat aactaatggt gtttgcgcag catctaaaat gattgagtcc
     7621 acttcatcaa taataccgta atttaattgt ggtaaaaatt tcccttccgc actatcagcc
     7681 aaattatcaa ttaaataatc aaaaccgaga cgtccattag ttgtatatat aatatcatgt
     7741 tcatataaat tacgtttttc ccctttttga tactcataat ccacaatatc aacaaaacct
     7801 aatgaagcag ttaaacctaa ccattcatat aatggttgca tctcttcaaa atcacgcttc
     7861 gctaaataat cattcgttgt aattaaatat gttccttttc ccgaaagtgc atttaaatat
     7921 aaaggcatcg ttgctgttaa tgttttacct tcgcctgttt gcatctccgc aatgttacct
     7981 tcatgcaata caatcgctcc gattaactga acttctttag gatacatacc taatactctc
     8041 cagctcgctt cacgtgccac tgcataagct tcaggtaaca atgtatctag tgtatcaact
     8101 cctgatgcta aacgttcttt aaattctatt gtcttttgtt ttaacgcatc atcagaatat
     8161 gatttaactt catcgcccca tgtattgatg cgtttcacta tttttctaat cgactttagt
     8221 cttaattcgt ttatcgtaac atctagttta tgtttcattt acttccccac cattcagttt
     8281 cgatacatct aagtaatcta aaaatcgtac tggattcatt aaacgtgaca tataatttag
     8341 atggttgtct tgctcttctt taaaataaac ctcgacattt gtatctttta gttcatgatt
     8401 tcctgggaca tgttctttaa gccattcttt taaatcatca tcttcatggc ttgtacgata
     8461 tactttgcaa tctaaatgct gagcgacata agttgcaaaa acatttgact ttgacccata
     8521 actaatcaaa ttaatagccg ttagggtatc ttgactttgc aaatcatcct ttagttgctt
     8581 aatattttcc ttgatatcgt cttccatcca acgttcgacg agccaaacat gaccaaacag
     8641 tttcaaaaaa tcattcgaaa tagttggata gacgtcagat ggttctgcaa taatgacatt
     8701 tattttatca tttccatatt ggttatcgtc tatcttcgtt acccgcatgc ttttatattc
     8761 taaatcatct tgatgcgtca tctctgtgat tgttaaacat ctaaatacaa gactcgtcga
     8821 tgctgcattc atcattttta ttttataagc ataggcttca tcaggatatt gaatcgtaat
     8881 actatttgac tttacaatct cagtacttag ttttgtgcca tttttattat aaaaaatgat
     8941 gataaaatac actgaaccag caggcgttgc atcaaaatca aaatgcaatt tataatgctg
     9001 tcctctacgc aaaattggta aacttggcgc acttttatat tttgaaaatt gctttaacat
     9061 caaccactca tgaatcggta atccagaggg catcaaaggg tttataaaag tcacttcacc
     9121 atttgaaaat gatactttag agccatacat aaatgtagtt tgtgaaatat aattccaagt
     9181 aactttaaat gttttgtttt tcagcatgtt gaactctccc aaacttgtct tccaaaataa
     9241 tgttgtaaaa attaacaaac caacttgcaa tggtaggtga atcatcatta tgtcgcccag
     9301 gaatactgcg attcatcact cttgcttggt gtgctgtcaa tacaggtaat agctcttgaa
     9361 atgcatttgg atcataatca tcatgttgca tatatgctat ggcaaaaaca gtttgtgaca
     9421 atgatttctt ttgaaatgtt tgccaaaatt tttgatttaa tgcctgtatc gacgcttgag
     9481 atgtatcacc ttcattagac accaggacgt ctaatgctgt accgaactct tctggtctaa
     9541 gtaatcgcat atgttcagca atcgttccaa tattaacaag tggtttacca acaataattg
     9601 cctgaggatt taactgagca ccataataca aggcaccaaa tgaacccata gataaaccag
     9661 ataatattaa ttcatgtgat ttaaaattca gcttttctaa tgtctcgtca ataacattga
     9721 taataccttg ttcatattca gatgaaccga tataaaaact accaccttca acacgaggat
     9781 cgccgataag taaaaacggt gcattcatac gtttcatcat ataatatcct tcgaaacctt
     9841 ccgctgttcg ataaccacta aaatatacgt ttagtggtgg tttcatatca ccagggtgga
     9901 aataataaat aaattcctgt cgttgactat ctacgaaacg actaccaccg agtaaaaatt
     9961 gacccatatc taatctagac catcgtttgt gtataggtcc taaatgtacc gttccgttgc
    10021 cacgcgcctt aacagttaca cttatataag catcaaatgg tttcgtaggt atctctaaag
    10081 gactgtctaa catatcatca gtcaatacga tttgttcaat taatgcacca tcagcgccag
    10141 tctgaatcaa tctaaatgta tattgcaact cgaccgcacc atcaatatca aattctggcc
    10201 atatttgaat gactttatct ttatcgtaaa cgagattatt ttgccaagat gcgataggtt
    10261 taaattcttt tccaaattct ccactcaatg tgagctctga attaccttgg taaacgacat
    10321 ctcctttaaa attcggatgc acaagtgcta acttaggaga aaccttatct ccatactgtc
    10381 ctgagaagct aactgcctct aatttattat tacgttcttc aatattccgg taatgtaatg
    10441 gttgaacaac gtatttttgg acattttcgt cttgttcata ttcaactgac caaaatgatt
    10501 catcaacata cgtattgtat ggttcactta tcatttgtaa taaattcgtt aatgtctccg
    10561 agtatggtgc ttgaatatag ataaaatcaa agcgcccttc tgcttcaaca atcgcttcaa
    10621 tagcctctac ataaccacta tcaaattcaa acaatccaat atcgaagtaa tcccaactca
    10681 cacctttttt gtgttgaaaa ataggttcta aatcgtctcc tccaatttgc aaaactctaa
    10741 atttacgtgg catcattttc accttctatt aactcatcga gctgattaat aatattctta
    10801 gaagcatatg catctattaa ttttaaagaa taggcgtacg cataattcca atttttcaaa
    10861 taaaataaat aataatttaa cgcatcatct aattcatcaa ctgtatttat aatacggcca
    10921 ttgtcataat cagagacgta atctgtttgt tgaccattaa tttgtggaat cccagcgcta
    10981 attgcactaa tttgtaaata caagtcaggt tcttttgaca tatctatcac aagtcgcaac
    11041 gtccgcaatg cttctacaac atcatgttca gcatgtatcg tcttaacagc aatgatgtca
    11101 tcttgatctt caggtgtcat taatgctgaa acattaacat ccgcattctg tttagcttgg
    11161 tattcctcat ttaccgacgt aatacattca cgaagccaca tcggtatgtc attttgatgg
    11221 cgcgataata aaattaaacg gtaataatct tcctgtgcga tataatccac aagtcgttgc
    11281 atcatttgtt gcaaatcagc gtcactcata ccatctatcc atacacctat aaatgtttcc
    11341 gtcaattgac tacttatatt aggtgattgt ctcgtttcaa atggtgtgat tcgaatcatt
    11401 gtattctccc gctgatactt ctcttgatga ctaattaaat cacgttctag ttgcacacta
    11461 tcaacaatta aatgggcatt ctttacgata gattgatatt cctcatctga cacagtttca
    11521 tttctatttt taaaaaatga ataacttaat gatttcgctg gaatatgatt ggctatttgt
    11581 cgattgtgcc tagcatctga agccacaatc acatgatcat cttcatgtat ttgttgtgca
    11641 atcattgctt gaaatttttc ttcaattagt tgagccatat tgttatattc tgtttgttga
    11701 tagtgatgtt gatatctttt tgaaacagtg actctgccat ttttcaaatc ttcatgaagt
    11761 acacaatctc cattaatcgt taaatattct tggtaagaag cctctccctg atcatcaaaa
    11821 taacgtatcg ctgataaata acctctgtca tcaaaaatat aacgccgttg taactgatct
    11881 ctttcaaatt cttcaaacca aattgaatac ccttcttgac taaaataaat atttgtatag
    11941 gtctgttcac tcgtcacaca ttttaataaa tacggtgtgt acacaaactc aacatcatcc
    12001 ggccatttta agtgatgata attaatcgct tgtggcgcat ggtggctgaa tccttgaatt
    12061 tcatcaaaca cagacgaata ctttgtctca tataagtcat atcgatgtaa aaatgttctt
    12121 aaatttggtg catgattgag aacaatcagt tgataatcta agtcattttc aaggtgcatt
    12181 cccattaaac taatcatatc gtcaaattcc gtcttatttt gtagttgata atacggcaca
    12241 gtcgtgtctt gccaccatcg ttggtcatcg taccaagctg gaataaagta tttcataatt
    12301 acctccttac caatactggc ttaaaaatgg cttatattta tcaaaatata aatatgtacg
    12361 aattgtttct gcaatattaa tactgatgta aactaataca attagttgta ctgagaaata
    12421 aatttcagta gataaatgcg gtacaaacaa tgtgaaataa agcggtatac caataatgat
    12481 tgtaactaat gccgatccaa accaacatac gcgtcgtgct tgataattta aataacgttc
    12541 tgtatcctta ccaggtttaa ctcctgaaaa ataattgcca ctctttaaga aatctttgga
    12601 tttttgttta gtattgatta aaaatctcga taaaaaataa cccaataaca tttgaatcac
    12661 taaatacacc gaaataccaa ttggactatc aaatgtcagc attggcatgt catctgatat
    12721 gcttttatta aacatagata aaataaaatg aatgccactt tttaagaaaa caaaagctga
    12781 aatgctcatc attaaagtaa tactgcctgc agggttaact ttccaagata aataagattt
    12841 catatttgtt gcggaaacgt tcattaaatc gatatatggt attctcactt ctactaattc
    12901 aataaataat aagataaaca atgtgattat cacaaggatg attaacaacg caatcacaat
    12961 atgacttgca tctatatatt ccattttttg atgcatcatt gatttaataa tgctaaccat
    13021 tacaatcggc attggtcctg caatgccgta acgactattt ttgtcagcta accaaactaa
    13081 taacatcgtt ccagtaacca aaatcaatat tgttaagtaa atattgtctt gatgaacacg
    13141 ttctttcgaa acatattcat gaatcacaaa ataactttga ataacactta aaattaatgt
    13201 taagatgcgc tctttataat gcttctcaag acttgtttgt ttcatgtatt tatccatatt
    13261 ccgataagaa atcagcatca agataatcat tgatgttaac cacggaccta atcctaaagt
    13321 gaaaatgttt aaagtattaa cgtctccacc catattagaa atagctattt taaaaaatga
    13381 ctcatgtttt acttgcatat cgttatagga aacgatggaa atgtttgtgc ctaatatata
    13441 aataaacaag ataaaacatg tgtatagcat acgtttatat ataattttat attcgtattg
    13501 ttgtaaaagt tttaacatgt tgcacctctt ttatatcaaa aacattaaaa agactaaggg
    13561 ttcatcacta attattaaaa tcctatatcg atttttctag tgattggtgc ctcagtcttt
    13621 ttaattttag ccagctataa attcaattta tgcttgagaa tcatcttgat cattttcatc
    13681 tttctttttc tttctcttca ttaaacctaa accaactaat aatgtcataa cgccacctag
    13741 taatccattt tgttttattg agtcacctgt atctggcaat cttttttcac tttgtgctgg
    13801 cgtgccatta tgtttagtca cttcagatgt tgcacttaat gtagactgag attcactcgt
    13861 gctcgttgtt gcttcacttg ataagcgaga tgtgctcgtg ctgtgagtat gatgcatact
    13921 cattgagtct gacggatgca ttgagttaga ttcagatgta cttgttgagc cggacatact
    13981 tgttgatgtc gagtcagaaa tgctttgtga accagacata gatgtactca gtgattcgga
    14041 tgtgcttgtc gaatcggatg tgctcaatga cgttgatgtg cttgttgaca ctgattctga
    14101 gtcgctcact gaagtcgagt tgtacgttga accggacata ctttgtgaca ttgaaacact
    14161 tgttgaatcg gattcactca ttgatgttga gtcagatacg ctcgttgaac ctgaaccaga
    14221 cgtacttaat gattcagata tgcttgttga agttgaacca cttgttgagt ccgatgtact
    14281 tgtcgatgtc gagtctgaat ctgatgtact caatgattct gagtcactga tagaagttga
    14341 atcacttgta gattctgatt ctactgtact ttgtgaacca ctgatactta ttgaagtaga
    14401 atcactgata ctgtctgatg ttgataatga tgtcgacacc gatgtgcttt gtgatgacga
    14461 tgtactagca ctcattgaca ttgatgttga tatcgatgta cttaaggaac cagatgcact
    14521 tgtacttgtt gactggcttt gtgacattga atcacttaat gatgtagatg tgcttgttga
    14581 gctcgagtca cttaaacttg ttgaacctga tattgagtca cttaaacttg tcgatgttga
    14641 aactgatacg cttccgctca ttgagtcaga tgttgaaagt gatgtactcg ttgaatttga
    14701 tccactgatg ctagacgaat cacttgtaga cattgagtcg ctttctgatg cactgatgct
    14761 catagagtca aattgactat tacttgttga gcttgactgc gaatcgctca cacttgttga
    14821 cgttgattct gatccactca tactttgcga gctactcaat gattttgaat cacttaatga
    14881 atccgaagtg ctaagacttg tggaaccact taaagatatt gatccactta atgagtcgga
    14941 gtcacttgta ctagtagaat cactcattga tattgaatca cttagcgagg tagactcgct
    15001 tacgctttct gaaccactta atgatgttga ggtactcaat gaaccagatg tacttgttga
    15061 agtcgaacca cttgtcgatt ttgaatcact taatgaatca gattcactca cgctttccga
    15121 acttcttagt gacgtcgata cacttaatga tgacgaatcg cttgtgctta ctgaatcgct
    15181 catcgattgt gagccactca atgaactaga ctcgcttaca ctttctgatt ttcttaatga
    15241 cgttgagacg ctcaatgagc cagaatcact tacgcttgtt gaaccactca tcgattttga
    15301 gtcactttca gaattagatt cgcttacact ttctgaatca tttacagatt ctgacatact
    15361 ttgtgaatca gatatgcttg cgctcattac ttcactagcc gatgttgatg tgcttgtcga
    15421 atcacttaac gatatagaca cactcatcga accagatgta ctcgcacttg ttgagtctga
    15481 tgttgaatca ctaacactat cagataatga cgttgaatca ctcatacttg ttgatgtact
    15541 tgttgaaagc gacatacttt gtgaatcact tgtacttgta cgcatcgaag tactagttga
    15601 agctgatgta ctacgagagt cacttgttga tgttgatgta cttgctgatc cagatgcact
    15661 tgtacttctt gatgtgcttt gtgaatcgct taatgatgtt gatgttgaat cggattcact
    15721 tgtactttct gatgttgagc cagattctga tgtacttacc gatgttgata aacttgcaat
    15781 ggtcgacatg cggtttgaag ttgatgtact tagcgaatca cttaatgatg ctgatgtgct
    15841 ttgtgaatcg gattcactgc tttgcattga cgcagatgtt gaatcactta aagatgttga
    15901 cgacgaatcg gattcacttg cgcttgctga gttactgagc gatgttgacg tacttgctga
    15961 tcctgagtta cttgtacttg ttgagtcact gagtgatgtt gacgcacttt ctgatgttga
    16021 ttcacttgca cttgttgaga tactttgtga atcagattca ctctctgaac cacttaatga
    16081 cgctgatgtg cttaccgaac ctgattgact cgtactttta gatatacttg ttgaatcgct
    16141 tagtgacgtt gatgtcgatg tggattcact ttggcttgtt gactgacttg tatatgatga
    16201 tgtgctttgt gaatcggatt cgctcgtgct tgtacttgtt gagtttgagg cactttggct
    16261 tgctgagttt gagtctactc cgctttgatt cattgatgca cttagtgaca atgacgtact
    16321 cgttgagtca gacaaacttg tactcgttga cgtacttgta cttcctgatg ttgattgaga
    16381 catacttatg ctcattgatg ttgaatcgga tttactttca cttgatgatg ttgagtcgga
    16441 ttcactttca cttgtagaac cacttaatga tgtggatgta ctaatggaat cagatttact
    16501 tgtactgatt gaatcacttg tcgacattga tgtacttaat gaatcagact tactatcact
    16561 tgtggaatca cttaatgatg ttgacaaact tgtagagtca gacaaacttg tactcgttga
    16621 catacttagt gatgttgaaa cactctcgct cttaaacgtt gacgttgatt cactgatact
    16681 tgtcgatgtt gaaatggacg cactaccact tgttgaatta cttaatgatg ttgatgtgct
    16741 accagattct gatgtactgt ctgataatga cgtactctca cttgtcgaac tactcactga
    16801 ctctgatgtt gattcagacg tactttcact taatgattca cttaaaaagg cagatgcact
    16861 ttgtgattct gaatcgctag tactatttga ttcacttaat gatataaacg tgctctctga
    16921 agcagatatt gcttcactta tagagtcgct cgttgacgtt gattcactta ttgaatcaga
    16981 ctctgatgta cttaagcttg tggaatcact catagatgtt gatgtacgtt ctgaattact
    17041 tgtacttaac gatgtcgaag tacttaccga accagatgtg ctcgtagaag cactttgtga
    17101 tattgattca cttgatgcag ttgatgctga tttgctatca ctcgttgaat cactaaacga
    17161 cgttgatatg ctcattgaat cggattgact tgcactcatt gaaccagacg tactttgtga
    17221 ttccgaagta cgtactgaag cacttgtcga cgttgatgta cttgttgaac ctgattgact
    17281 agtacttagt gataatgact tactatcaga atcagatgta ctttgtgaat cacttaatga
    17341 ttctgatgta ctacctgact gagacgtgct cattgaacta cttacagaca ttgatttact
    17401 gtctgatgca gataatgacc cacttgtact gatagagtca cttactatct ctgaagtact
    17461 catcgagtct gatgtacttg ttgagacact ttgtgatgct gctatgctta gtgatccaga
    17521 aacagaacca cttgtgctcg tcgaatcgct caatgattct gatgtactca tcgattttga
    17581 atcacttgta cttaatgata ttgatgtact ttgtgaatct gatttgcttg ttgacgcact
    17641 ttgagagttg gctatgctat ttgaaatact gatagagtcc gaggtgctag ctgactcgct
    17701 caatgattct gatgtgctga ttgcattcga tgtgctgtca cttaatgatg ctgatgtact
    17761 agacgattct gatgtacttg ttgatagact ttgtgactta gacaagctcc ccgatgtact
    17821 catacttaat gagtcactga gtgacgttga tgtacgcaat gaatcagatg tacttgttga
    17881 tagactttcg gatttttcag tactgctaga gtttgaaata gaatcgctta atgatgttga
    17941 tttactagct gaatccgaca tgcttgatga tacactttgt gaattcacta aacttgtgct
    18001 tgttgagctt gatacactat tactttcaga tgtgcttaat gacttagatg cactcacaga
    18061 atcagatagg cttacacttg tcgatttcga ggtactagct gatgtagata ccacaatcga
    18121 tcctgatgta ctcgttgatg cactttgtga gtcagcttta cttgttgaga cactttggga
    18181 ttgttgtgta cttcctgatg ttgatacgga atcactcatg ctatttcttg ttacttcata
    18241 tttaaaagtt gttgtcgttt tgttaccact cgcatctgta gaaacgattg taatagtact
    18301 tgtaccaatg tttgttggtg taccactaat ggtattattt gtactatcga atgttagtcc
    18361 agatggcaat ccagtcactg tattcgtcac cgcatttcca ctgttgtcct gcgtagcaat
    18421 tttaatcggt gatattggcg aatacacttc tgatgattga tctcctattg gtgtcactgt
    18481 tggtgctgtc gtatccacaa catttattgt aaaagttgtc gtcgatttat tgtttgcttg
    18541 gtcagtagac acaactgtca ctgttgattg accaattttc gttggcgtcc caatgattga
    18601 attcgttgca ctatcataac ttaatccgct tggtaatcct gtaactgtat ttgtcacagt
    18661 cccagtacca ttatccgttg tagtcaacac aacaggattc attgttttac ccacttcaat
    18721 ggtttgattg cctacagtta cagttggtgc ttttacatca gtaaaataat atgtcactga
    18781 ttgtccagca ttcgtcattt ttacagtttt atttgtatca ttataagttg acgcatatga
    18841 actatcaacg gacgtgtagt tatatccttt agcagtcaat gcagattgct gattatcgat
    18901 tgtcacgact tgatcaacat ttcctgaata tgtttttggt ggaataatat ctttacctgt
    18961 tgttacatca acgtatctca cttgtgtaac agcagactct gtatattcga atgttccaaa
    19021 ttgtacttgt tgtaaatttg tcgcgccacc tgttgaggct gtcattgata atgaaaagtt
    19081 ggtcgtacca ctttttgcaa tccaatctga aatattacgt gtccatgttt gacctgcata
    19141 cgttacagtc ataacttttg tatcaccatt ataattaata tcaaaatctt ggaacgtgtt
    19201 gttcgtaggt tgcactttta acttcgcagc attatcagct gttgaacttg atgtatacgt
    19261 tgtcgcaaca ccataactat ctgttgttac aaaggcacca aacgcacctc caccagctac
    19321 attagatggg tcagcattcg cctttgcagc tgaatttggt ttagatgtat tgtgatacgt
    19381 atccaatttg aagccaaatg cgttacttaa gccaccaata cctactgcgg caccgtttaa
    19441 ccctgtttca cctaatacac ctggcgaaaa ggcaaagccg ataccatctc caccatttcc
    19501 attaccttcg tatttattac ctaaatttac ttttccagaa aaatgaaaac tcttattaga
    19561 gtcaatacgt gttcctaatg taatggcacc tttttggctg tatgcatcct gtgttaacgt
    19621 cacaataccg gtactttgat cataggtagc attacctgac gttgtcatat attgttttaa
    19681 gttatcttta ttaactgtaa ttgtattagc agttactgcg gttgtcgtcg ctgctgacgc
    19741 aaatgttgac atagctaagc gactgaaagt tcgaagtttt actggtgcgg tgctagttga
    19801 cgttgtgcta gttttgttta agttgaccga agatggcgtt gtgctttgtg aagtgttatt
    19861 tgatgcagta ctttgatttg ttgatgtatt aattggttgt tctgtacttg aagttgaagc
    19921 tacagattta gtatcagaac ttgatgtagt attctttgag gatgttgatt ctgatgtaga
    19981 tgtcaatttt tcttgttgat tgcttgtact attagttgtc gaagtgacct tttcagactt
    20041 ttcacttgag actgtgtcac tatttgatgt ttgtaccgaa ctactatttt tcgttacact
    20101 tgtggaatcg gctgttgatg ttgatgcttc gattgtcgtt gagttttgat tacctactgt
    20161 ttcactttgt gtgtttaatt cagaagttaa tggtgcatca gaagccgcaa aagcttgctg
    20221 gtcatgcaac atatttaccg tgaatgcacc accaataacc gccgtagttt tcagtccgta
    20281 tcccgtcatt tttttactaa tgctttgatt atcttgactc actaaactat gactaataaa
    20341 tggtagcccc ataattttga acatttctat ttctttaatt ccggatttta cccaattttt
    20401 tccagattta taaagtctta ctcttgtttt ttcgtttgct aagctgtcat gaaattcttt
    20461 ctgtctttta ctcatgtaat aactccttgt attatcttta cattcattag attataatat
    20521 atgccactat tcaatttaat acaactcttt tttgatacaa
//
LOCUS       DBNENW010000038        20035 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNENW010000038 DBNENW010000000
VERSION     DBNENW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.038"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            18..1547
                     /gene="aur"
                     /locus_tag="AC1LW8_001069"
     CDS             18..1547
                     /gene="aur"
                     /locus_tag="AC1LW8_001069"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HIP4772919.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1914..2363
                     /locus_tag="AC1LW8_001070"
     CDS             1914..2363
                     /locus_tag="AC1LW8_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP4772920.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2366..2533
                     /locus_tag="AC1LW8_001071"
     CDS             2366..2533
                     /locus_tag="AC1LW8_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772921.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGIFE"
     gene            2710..3945
                     /gene="arcA"
                     /locus_tag="AC1LW8_001072"
     CDS             2710..3945
                     /gene="arcA"
                     /locus_tag="AC1LW8_001072"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIP4772922.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            3978..4988
                     /gene="argF"
                     /locus_tag="AC1LW8_001073"
     CDS             3978..4988
                     /gene="argF"
                     /locus_tag="AC1LW8_001073"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIP4772923.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5079..6509
                     /gene="arcD"
                     /locus_tag="AC1LW8_001074"
     CDS             5079..6509
                     /gene="arcD"
                     /locus_tag="AC1LW8_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HIP4772924.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6526..7467
                     /gene="arcC"
                     /locus_tag="AC1LW8_001075"
     CDS             6526..7467
                     /gene="arcC"
                     /locus_tag="AC1LW8_001075"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIP4772925.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7566..8270
                     /locus_tag="AC1LW8_001076"
     CDS             7566..8270
                     /locus_tag="AC1LW8_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIP4772926.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLFCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8620..11271
                     /gene="clfB"
                     /locus_tag="AC1LW8_001077"
     CDS             8620..11271
                     /gene="clfB"
                     /locus_tag="AC1LW8_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HIP4772927.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNGANVDSTAKPMST
                     QTSNTTTTEPASTNETPQLTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTAKDFQLEKTTFDPNQSGNTFMAANFTVTGQVKSGDYFTAKLPDSVTGNGDVDYSNS
                     NNTMPIADIVNDKNEVVAKATYDILTKTYTFVFTDYVNDKQNINGKFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKDKITYKYQNVASINFGDINKTYVVLVEGHYDKTGKNLKTQVIQEN
                     VDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPEPTPDPEPSPDPDPDSDSDSDSGSDSDSDSDSDSDSDSDSGSDSDSDSDSDSES
                     DSESDSDSDSESDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     ESDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNA
                     TLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            11387..12148
                     /locus_tag="AC1LW8_001078"
                     /pseudo
     CDS             11387..12148
                     /locus_tag="AC1LW8_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            12393..12848
                     /locus_tag="AC1LW8_001079"
     CDS             12393..12848
                     /locus_tag="AC1LW8_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4772928.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            12970..13071
                     /locus_tag="AC1LW8_001080"
     CDS             12970..13071
                     /locus_tag="AC1LW8_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE2200 family small protein"
                     /protein_id="HIP4772929.1"
                     /translation="MKKLAVILTLVGGLYFAFKKYQERVNQAPNIEY"
     gene            complement(13131..14555)
                     /locus_tag="AC1LW8_001081"
     CDS             complement(13131..14555)
                     /locus_tag="AC1LW8_001081"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIP4772930.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKNDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAKVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            14753..14953
                     /locus_tag="AC1LW8_001082"
     CDS             14753..14953
                     /locus_tag="AC1LW8_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772931.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            14979..15644
                     /gene="nsaR"
                     /locus_tag="AC1LW8_001083"
     CDS             14979..15644
                     /gene="nsaR"
                     /locus_tag="AC1LW8_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HIP4772932.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            15655..16542
                     /gene="nsaS"
                     /locus_tag="AC1LW8_001084"
     CDS             15655..16542
                     /gene="nsaS"
                     /locus_tag="AC1LW8_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HIP4772933.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLLIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            16650..17405
                     /locus_tag="AC1LW8_001085"
     CDS             16650..17405
                     /locus_tag="AC1LW8_001085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772934.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            17402..19402
                     /locus_tag="AC1LW8_001086"
     CDS             17402..19402
                     /locus_tag="AC1LW8_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772935.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFIIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDFVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         7236 a         2961 c         3661 g         6177 t
ORIGIN      
        1 ttcaggagga atgaaatgtg aggaaatttt caagatatgc atttacaagt atggcagcag
       61 taacattgtt gagcacttta tcaccagcag cattagcgat tgattcaaaa aataaaccag
      121 ctaattctga tattaaattt gaggtgactc aaaagagtga tgcggtcaaa gcattaaaag
      181 aattgcctaa atccgaaaat gtaaaaaata tttatcaaga ttacgctgtt actgatgtaa
      241 aaactgataa aaaaggattt acgcattata cattgcaacc gagtgttgat ggtgttcatg
      301 cacctgacaa agaagtgaaa gtacacgcag acaaatcagg aaaagtcgtt ttaatcaatg
      361 gggatactga tgcgaagaaa gtaaagccaa cgaataaagt gacattaagt aaagatgacg
      421 cagccgacaa agcatttaaa gcagttaaga ttgataagaa taaagcgaaa aatcttaaag
      481 ataaagtcat taaagaaaac aaagttgaaa tcgatggtga cagtaataaa tacgtttata
      541 atgttgagtt aattacagtg acaccagaaa tttcacattg gaaagttaaa attgatgctc
      601 aaactggcga aattttagaa aaaatgaact tagttaaaga agctgcagaa actggtaaag
      661 gaaaaggtgt acttggcgat acaaaagata tcaatatcaa tagtattgac ggtggattta
      721 gcctagaaga tttaacgcat caaggtaaat tatcagcatt tagctttaat gatcaaacag
      781 gtcaagcaac attgattact aatgaagatg aaaacttcgt aaaagatgag caacgtgctg
      841 gcgtagatgc aaattattac gctaaacaaa catatgatta ttacaaagac acatttggtc
      901 gtgaatcata tgacaaccaa ggtagtccaa ttgtttcatt aacgcatgtt aataactacg
      961 gtggtcaaga taacagaaat aatgccgcat ggattggtga caaaatgatc tatggtgatg
     1021 gtgatggtcg cacattcaca agtttatcgg gtgcaaatga cgtagtagca cacgaattaa
     1081 cacacggtgt gacacaagag actgcaaact tagaatataa ggaccagtca ggcgctctaa
     1141 atgaaagctt ttcagatgtt tttggatact ttgtagatga cgaggatttc ttaatgggtg
     1201 aagatgtcta cacacctgga aaagagggag acgctttacg cagcatgtca aacccagaac
     1261 aatttggtca accagctcat atgaaagact atgtattcac tgaaaaagat aatggtggcg
     1321 tacatacgaa ttctggaatt ccaaataaag cagcttataa cgtgattcaa gcaataggga
     1381 aatctaaatc agaacaaatt tactaccgag cattaacgga atacttaaca agtaattcaa
     1441 acttcaaaga ttgtaaagat gcattatacc aagcggccaa agatttatat gacgagcaaa
     1501 cagctgaaca ggtgtatgaa gcatggaatg aagtaggcgt ggagtaaaaa tatataaaca
     1561 agaagaagta atgttaaaca cttataaata attaaatttt aaatacagct tatatatggg
     1621 gtatacctac attgataatt gactttgtag gtataccctc tctttgtaat tagatgagag
     1681 gagtgtgagg gttgtctgcc gaaagactac tcggcagtct aaaatcatta caagtagtag
     1741 atatgtgata attaaatgct gacttagaat acaaaattca ttttaaaagt tgtcacaaaa
     1801 aatttacatg tatttttatt atcttttgca aaacaaagtg ttaaattata aatgaaacat
     1861 gcatgaattt attttttaat acaagaaacg taactaccaa aggagtttac aatatgaaga
     1921 aaagtaaacg attagaaatt gtttctacaa tagttaaaaa gcataagatt tataaaaaag
     1981 aacaaatcat ttcatatatt gaagaatatt ttggtgtaag atatagtgca acaacaattg
     2041 ctaaagactt gaaagaactt aatatatatc gtgtccctat cgattgtgaa acatggattt
     2101 ataaagctat taataatcaa acagaacaag agatgagaga aaagtttaga cactattgtg
     2161 aacatgaagt tctaagttca atcatcaatg gttcatacat tatcgttaaa acctcacctg
     2221 gtttcgccca aggcataaac tattttatcg atcagctaaa tatagaagag atattaggta
     2281 cggtgagtgg aaatgacact acattaatct taactgcctc aaatgatatg gcagaatacg
     2341 tatatgcaaa attatttaaa tagacatgta tcaaatgaat aataaaaatt tgtttcgtat
     2401 cacgtgtact caagttagtt accaaatatt aacttgtgta cgcgtttttt tatggaaaga
     2461 aagaattcat agtcattcaa ttgactgtat aaaaaacttt atacaacatg tttttatggg
     2521 tatttttgaa taaaaaatgt atattttgac ccaaaatacc tttatttatg tataaaaatc
     2581 cattattatg tattgtataa caaaaagata tgaaattttc gactttcttt atgtgaatat
     2641 aatcacatgt aagcgtttga agattgtcta tactctaaat gaattcaaag ataaaaggag
     2701 gaaatagaca tgacagatgg tccaattaaa gtaaatagcg aaattggagc tttaaaaact
     2761 gtgttactta agcgtcctgg aaaagaatta gaaaatttag tacctgatta tttagatgga
     2821 ttactatttg atgatattcc atatttagaa gtagctcaaa aagagcatga ccattttgcg
     2881 caggtgctaa gagaagaggg tgttgaagta ctttaccttg agaagttagc agctgaaagt
     2941 attgaaaatc ctcaagtaag aagtgaattt attgatgatg tattagcaga gtctaaaaaa
     3001 acaatattag gtcatgaaga agaaattaag gcattatttg cgacactttc taatcaagaa
     3061 cttgtagata aaataatgtc aggggtacgt aaggaagaaa ttaatccgaa atgtacacat
     3121 ctagtagagt atatggatga taagtatcca ttctatttag atccaatgcc aaacctttat
     3181 tttactagag atccacaagc ctcaatagga cacggtataa caatcaatcg gatgttctgg
     3241 agagcacgac gacgagaatc aatatttatt caatatattg taaagcatca tcctagattt
     3301 aaagatgcga atattccaat ctggttagat cgagattgcc cattcaatat tgaaggcggc
     3361 gatgaacttg ttttatctaa agatgtcttg gctataggcg tttcagaacg tacatctgca
     3421 caagctattg aaaagttagc gcgacgtatt tttgaaaatc cgcaggcgac gtttaaaaaa
     3481 gtagtagcaa ttgaaattcc aactagtcga acttttatgc acttagatac agtatttaca
     3541 atgatagatt atgacaaatt tacaatgcat tcagccattt taaaggcaga aggcaatatg
     3601 aatatattta ttattgaata tgatgacgta aataaagata ttgccatcaa acaatctagt
     3661 catttaaaag atactttaga agacgtacta ggtatagatg atatccaatt cattccaaca
     3721 ggaaatggtg atgtcattga tggtgctaga gagcaatgga atgatggctc aaatacatta
     3781 tgtataagac caggcgttgt agtgacttac gatagaaact atgtatcgaa tgatttattg
     3841 agacaaaaag gcattaaagt cattgaaata tctggtagcg agttggtacg tggacgtggg
     3901 ggccctagat gtatgagtca accattattc agagaagaca tttaacgtaa taaattatag
     3961 aaacgagggt gaaaataatg acagaaattc aaaaaccgta tgatttaaaa ggcagatcat
     4021 tattaaaaga aagtgatttt accaaagcag aattcgaagg acttattgat tttgcaatta
     4081 cattaaaaga gtataagaaa aacggtatta agcatcacta cttatctgga aaaaatattg
     4141 cactactatt cgaaaagaat tcgacgagaa cgcgtgctgc gtttacagtt gcgtctattg
     4201 atttaggtgc gcatccagaa tttttaggga aaaatgatat tcaattaggc aaaaaagaat
     4261 ctgtagagga tactgcgaaa gtattaggta gaatgttcga tggtattgaa ttccgtggtt
     4321 tttcacaaca agctgttgaa gatttagcga agttctctgg tgtgccggtg tggaatggat
     4381 taacagacga ttggcatcct acacaaatgt tagctgattt tatgacaata aaagagaatt
     4441 ttggatatct agaaggaata aacttaactt acgttggaga tggacgtaat aatattgcgc
     4501 attcattaat ggtagcaggt gctatgttag gtgttaatgt aagaatttgt acacctaaat
     4561 cattaaatcc aaaagaggca tatgttgata ttgcaaaaga aaaagcgagt caatatggtg
     4621 gttcagtcat gattacggat aatattgcag aagcagttga aaatacagat gctatatata
     4681 cagatgtttg ggtatcgatg ggtgaagaaa gtgaatttga acaacgtatt aatttattaa
     4741 aagactatca agtgaatcaa cagatgtttg atttaacagg taaagattca acgatattct
     4801 tacattgttt accagcattc catgatacaa atacacttta tggacaagaa atttatgaaa
     4861 aatatggatt agctgaaatg gaagttacag accaaatctt tagaagtgaa cattcaaaag
     4921 tgtttgatca agctgaaaat agaatgcata caattaaggc agtaatggca gcaacattgg
     4981 ggagttaatc actaaatgga acgatatgaa tatgatgtgt ctgatgatat aagtgtcatg
     5041 tacagacacc tcatattggt attaaaggag aaatgaatat gaacgaatca ggagataaca
     5101 aactcagtaa atcttcttta attggactag ttataggatc catgattggt ggcggtgcgt
     5161 tcaatataat gtctgatatg ggcggtaaag ccggtggatt agccattatt attggttgga
     5221 ttattacagc tataggaatg atttcattag cgttcgtatt tcaaaattta accaatgaac
     5281 ggccggagct agacggtggt atttatagtt atgctcaagc aggatttggc gattttgtag
     5341 gatttatcag tgcttgggga tattggttct cagcgttttt aggcaatgtt gcctatgcaa
     5401 cactattgat gtcagcagta ggtaactttt tcccgatttt taaaggaggc aacacattac
     5461 caagtattat tgtcgcctcg ttactactct ggggtgtcca tttcttgatt ttaaaaggcg
     5521 ttgaaacagc agcatttatc aatagtattg ttactgttgc aaagttaata ccgattttac
     5581 ttgtaatcat atgcatgata attgcattca attttgacac ttttaaaaca ggctttttcg
     5641 gtatgacgtc agagggtgta ttgccattta gttgggcgag cacaatgagc caagttaaaa
     5701 gtacgatgct agtgacagtt tgggtgttta tcggtatcga gggtgcagta attttttcta
     5761 gtagagctaa aaataaaaaa gatgtaggta gtgccacggt tataggactt atatcagttt
     5821 taattatcta tttcttatta actgtattag ctcaaggcgt gattttgcaa aatcatattt
     5881 ctcaattaga ttcgccaagt atggcacagg tgcttgcaac tattgtaggt ggttggggat
     5941 ctacacttgt aaatattggt ttaattattt cggtactagg tgcatggtta ggatggacat
     6001 tgcttgctgg tgaattacct ttcattgttg caaaagatgg attatttcca aagtggtttg
     6061 ctaaagaaaa taaaaatgga gcacctgtaa atgcactgct tattaccaat atattagtac
     6121 aattattttt aataagtatg ctatttacac agagtgcgta tcaatttgca ttttcactag
     6181 catcaagtgc tattttatac ccttacatgt tcagtgcatt ttaccaagtt aaatacactt
     6241 tagagcatcg acagcaagca actactaagc aatggacgat tggcatcata gcctcaattt
     6301 atgctatatg gcttatatat gcagcaggta tcaattactt attattgacg atgttacttt
     6361 atattccagc tcttcttgtt tattcaatcg ttcaaaagaa taatcagaca cgtttgatta
     6421 aatcagacta tattcttttt atgattatta tcgtacttgc agttatcgga ttaattaagt
     6481 tattgatggg aacgataaat gttttttaaa aggagcgaca aaaatatgaa agagaaaatt
     6541 gtcattgcat taggcggtaa tgcgatacag acaaaagaag caacagctga agcacaacaa
     6601 acagctatta gacgtgcgat gcaaaacctt aaacctttat ttgattcacc agcgcgtatt
     6661 gtcatttcac atggtaatgg cccacaaatt ggaagtttat taatccaaca agctaaatcg
     6721 aacagtgaca caacgccggc aatgccattg gatacttgtg gtgcaatgtc acagggtatg
     6781 ataggctatt ggttggaaac tgaaatcaat cgcattttaa ctgaaatgaa tagtgataga
     6841 actgtaggca caatcgttac acgtgtggaa gtagataaag atgatccacg attcaataac
     6901 ccaaccaaac caattggtcc tttttatacg aaagaagaag ttgaagaatt acaaaaagaa
     6961 cagccagact cagtctttaa agaagatgca ggacgtggtt atagaaaagt agttgcgtca
     7021 ccactacctc aatctatact agaacaccag ttaattcgaa ctttagcaga cggtaaaaat
     7081 attgtcattg catgcggtgg tggcggtatt ccagttataa aaaaagaaaa tacctatgaa
     7141 ggtgttgaag cggttataga taaagatttt gctagtgaga aattagcaac gctgattgaa
     7201 gcagatacct taatgattct tacgaatgta gaaaatgtat ttattaactt taatgaacct
     7261 aatcaacaac aaatcgatga tattgatgta gcaacactga aaaaatacgc ggcacaaggt
     7321 aagtttgcgg aaggatcgat gttgccaaaa atagaagctg cgatacgatt tgttgaaagt
     7381 ggggaaaaca aaaaagttat cattaccaat ttagagcagg catacgaagc tttgattggt
     7441 aataaaggta cacacattca catgtagcac tttatcacgc gacaaaacat taaatatgtt
     7501 tctccgttga ttcaaatgaa aaagttgtct gctgacactt tgcaaggttt gaaggagttt
     7561 aacttatgac agaaaacttt attttgggta gaaataataa attagaacat gaactaaagg
     7621 cattagcaga ttacattaat atcccatata gtatattaca accatatcaa agtgaatgtt
     7681 ttgtcagaca ttatacgaaa ggccaagtta tttatttttc gccacaagaa agtagcaata
     7741 tttacttttt aattgaaggt aacattatta gagaacatta caatcaaaat ggagatgtat
     7801 atcgttattt taataaagag caagtattat ttccaatcag taacttattt catccgaaag
     7861 aggttaacga attgtgtaca gcattaaccg attgtacagt tcttggattg cctagagaat
     7921 tgatggcctt tttgtgcaaa gctaatgatg atatattttt gacacttttt gcattaataa
     7981 atgataatga gcagcaacac atgaactata acatggcatt aacaagtaaa tttgctaaag
     8041 atcgaattat taaattgttt tgtcatctat gtcagacagt agggtacgat caagatgaat
     8101 tttatgaaat caaacagttt ttaactattc aactcatgag tgatatggct gggatttccc
     8161 gggaaacagc tggtcatatt attcatgaac ttaaagatga aaaacttgtt gttaaagatc
     8221 ataaaaattg gttagtaagc aaacatttat tcaatgatgt atgtgtttaa tatacaatgt
     8281 aaaatgaata agttgaacat gaggtctaac gtacatttat acgttaggcc ttttttgcta
     8341 gcatgatgaa taatttaaaa tgttagttaa atttgattgt tgaaattaca gtaaaattta
     8401 aggtgatgaa aaatttagaa cttctaagtt tttgaaaagt aaaacatttg caatagtgta
     8461 aaaatggtat attgattttt gctagttaac agaaaatttt aagttatata aataggaaga
     8521 aaacaaattt tacgtaattt ttttcgaaaa gcaattgata taattcttat ttcattatac
     8581 aatttagact aatctagaaa ttgaaatgga gtaatatttt tgaaaaaaag aattgattat
     8641 ttgtcgaata agcagaataa gtattcgatt agacgtttta cagtaggtac cacatcagta
     8701 atagtagggg caactatact atttgggata ggcaatcatc aagcacaagc ttcagaacaa
     8761 tcgaacgata caacgcaatc ttcgaaaaat aatgcaagtg cagattccga aaaaaacaat
     8821 atgatagaaa cacctcaatt aaatacaacg gctaatgata catctgatat tagtgcaaac
     8881 acaaacggtg cgaatgtaga tagcacagca aaaccaatgt ctacacaaac gagcaatacc
     8941 actacaacag agccagcttc aacaaatgaa acacctcaac tgacggcaat taaagatcaa
     9001 gcaactgctg caaaaatgca agatcaaact gttcctcaag aagcaaattc tcaagtagat
     9061 aataaaacaa cgaatgatgc taatagcata gcgacaaaca gtgagcttaa aaatcctcaa
     9121 acattagatt taccacaatc atcaccacaa acaatttcca atgcgcaagg aactagtaaa
     9181 ccaagtgtta gaacgagagc tgtacgtagt cttgcagttg ctgaacctgt agtaaatgct
     9241 gctgatgcta aaggtacaaa tgtaaatgat aaagttacgg caaaagattt tcaattagaa
     9301 aagactacat ttgaccctaa ccaaagtggt aatactttta tggcggcaaa ctttacagtg
     9361 actggacaag tgaaatcagg ggattatttt acagcgaagt taccagatag tgtaactggt
     9421 aatggagacg tggattactc taattcgaat aatacgatgc caattgcaga tatagtaaac
     9481 gataaaaatg aagttgtagc aaaagcgaca tatgatattt tgactaagac atatacattt
     9541 gtctttacag attatgtaaa tgataagcaa aatattaatg ggaaattttc attaccacta
     9601 tttacagaca gagcaaaggc acctaaatca ggaacatatg atgcaaatat taatattgcg
     9661 gatgaaatgt ttaataataa aattacttat aactatagtt cgccaattgc aggaattgat
     9721 aagccaaatg gcgcgaacat ttcttctcaa attattggtg tagatacagc ttcaggtcaa
     9781 aatacataca agcaaacggt atttgttaac cctaagcaac gagttttagg taatacgtgg
     9841 gtgtatatta aaggttacca agataaaatc gaagaaagta gcggtaaagt aagtgctaca
     9901 gatacaaaac tgagaatttt tgaagtgaat gatacatcta aattatcaga tagctactat
     9961 gcagacccaa atgactctaa tcttaaagaa gtaactgatc aatttaagga taaaatcact
    10021 tataaatacc aaaatgtagc aagtattaat tttggtgata taaataaaac gtatgtcgtt
    10081 ttagtcgaag gtcattatga taaaacaggt aaaaacttga aaacgcaagt cattcaagaa
    10141 aatgttgacc cagcgacagg taaagactac agtattttcg gttggaataa tgagaatgtt
    10201 gtacgttatg gtggtggaag tgctgatggt gattcagcag taaatccgaa agacccaact
    10261 ccagggccac cggttgaccc agaaccaagt ccagacccag aaccggaacc aagtccagac
    10321 ccggatccgg aaccaacgcc agatccagaa ccaagcccag acccggatcc ggattcggat
    10381 tcagacagcg actcaggttc agatagcgat tcggattcag acagcgattc agactcagac
    10441 agcgactcag gttcagatag cgattcggat tcagacagcg actcagaatc agacagtgag
    10501 tcagattcag atagcgattc agaatcagat agcgactcag attcagatag tgagtcagat
    10561 tcagacagtg actcggactc agatagcgat tcagactcag acagcgattc agactcagac
    10621 agcgactcag attcagatag cgactcagac tcagacagcg attcagaatc agatagcgac
    10681 tcagattcag atagtgagtc agattcagat agtgactcgg actcagatag cgattcagac
    10741 tcagacagcg attcagactc agacagcgac tcagactcag atagcgactc agattcagat
    10801 agtgagtcag attcagacag tgactcagac tcagatagcg attcagactc agacagcgat
    10861 tcagattcag acagcgattc agactcagac agcgactcag actcagacag cgactcagaa
    10921 tcagatagcg attcagactc agacagcgat tcagattcag atagcgactc agactcagac
    10981 agcgattcag attcagacag cgactcagac tcggatagcg actcggattc agatagtgac
    11041 tccgattcaa gagttacacc accaaataat gaacagaaag caccatcaaa tcctaaaggt
    11101 gaagtaaacc attctaataa ggtatcaaaa caacacaaaa ctgatgcttt accagaaaca
    11161 ggagataaga gcgaaaacac aaatgcaact ttatttggtg caatgatggc attattagga
    11221 tcattactat tgtttagaaa acgcaagcaa gatcataaag aaaaagcgta aatacttttt
    11281 taggccgaat acatttgtat tcggtttttt tgttgaaaat gattttaaag tgaattgatt
    11341 aagcgtaaaa tgttgataga gtagaattag aaaggggtca tgacgtatgg cttatatttc
    11401 attaaactat cattcaccaa caattggtat gcatcaaaat ttgacagtca ttttaccgga
    11461 agatcaaagc ttctttaata acgatataac tgctaaacca ttaaaaactt taatgttgtt
    11521 acatggatta tcaagtgatg aaacgacata tatgagatat acaagcatag aaaggtatgc
    11581 gaatgaacac aaattagctg tgattatgcc caatgtggat catagcgcat atgctaacat
    11641 ggcatatggt catagctatt atgattatat tttggaagta tatgattatg ttcatcaaat
    11701 atttccactt tccaaaaagc gtgatgacaa ttttatagca ggtcactcta tgggaggata
    11761 tggcacaatt aaatttgcat taacacaagg ggataaattt gccaaagctg taccattatc
    11821 tgctgtgttt gaagcgcaaa atttaatgga tctagagtgg aatgattttt caaaagaggc
    11881 cataattggc aatctttcaa gtgttaaagg aactgaacat gatccgtatt atttgctaga
    11941 caaagctgta gctgaagata aataaattcc aaaattgctc attatgtgtg gtaaacaaga
    12001 ctttttatat caagacaact tagattttat cgattattta tcacgtaaaa atgttcctta
    12061 tcaatttgaa gatggaccag gagatcatga ttatgcatat tgggaccaag cgattaagcg
    12121 tgctataaca tggatggtga atgattaatt atttcctgga aaatatgtgg ttgcattaaa
    12181 tacacagagt gagagataca aactatttac gcacgactaa catttctgag tgtttaaatt
    12241 aaatttgtat taatatgatt ggcgcaattt gctgatacgc aaaaatgttt ctcgtgaaac
    12301 ttagatttag cttatagttt tatcatcatt tgtatgactt acattataaa ttttattata
    12361 atgaggttaa cgctttgaaa ggagtcatca tcatgtcgac caataaaaac gattatgagc
    12421 atatgttgtt ttattttgca tataaaacgt ttattactac cgctgatgaa attatagaga
    12481 agtatggtat gagccgtcag catcatcgtt ttttgttttt tatcaataaa ttacctggta
    12541 ttactattaa atcattacta gaaatattag aaatttctaa acaaggatca catgcaacac
    12601 ttcaaaaatt aaaagagcaa ggtctcatta ttgaaaaagt tttagagact gatcgacgtg
    12661 tcaaaaaatt atattcgacg gataaaggcg atcaactcat tgctgaattg aacaaggcgc
    12721 aagatgaatt attgcaaaat atatatcaac aagtcggttc ggattggtat gatgtgatgg
    12781 aagcattggc taaagggcga cctggctttg attttattaa gcatttgaaa gatgaaaaag
    12841 aaagctagca tcagaaatgt taaaaatctt cgcattctta aatttaaaaa atatgtcaaa
    12901 aagtgtataa taaaaacata taatttaatt gaactcagtt tcaacacatc ttagaaagga
    12961 gtttgaatga tgaaaaaatt agcagttatt ttaacattag ttggcggttt atacttcgca
    13021 tttaaaaaat accaagaacg tgttaaccaa gcacctaaca ttgagtacta aattaaacca
    13081 taaaaaattc ccgaacacct tgttatagtg ctcgggaatt tttttatgct ttacttgaat
    13141 atatcaaata ttatttttgc gctttctgta ttttcgatat taccactaaa tgattctgat
    13201 ctaggtccat aagcgtaggt attaacatcc tcgcctgtat gtccatcgga agtccaccct
    13261 gtataagatt tatcatttac tggcttctga atagcgtgtt gtagggcttt tgtttgcgtt
    13321 tctacttttg cggatttttc gtctttttct tttttaagta gtctttttag ctttttattc
    13381 tcttttttaa cctttttcat atcatcttgt gaaaattcaa agccataacc ttcattaata
    13441 actttttcag ggtcttcacc tttagccatt ttttctgtca tatatgatcc agagtgtttc
    13501 atcgatttaa tcggttgagg attccattcg tatcctttat ctttaccaat tgttagacca
    13561 ccagtagagt gatctgctgt tgcaacgaca agtgtatctt tatgtttctt agcatattga
    13621 atggcgtcat cgaatgcttt ttcaaaacct tccatttcag acataacgcc tgtaatatcg
    13681 ttggaatgcg ctgatttatc tatagaagca ccttcgacca ttaaaaagaa tcctttttta
    13741 ttacgctcaa gcttactaag tgcactttgt tgcatatcag ctaatgatgg ttcatcttta
    13801 gaagcatcta ttgcaagtgg catattttta tctgcaaaca aaccaagaac tttatcttta
    13861 tcagattttg ataactcctt actgtttgtg gcaaggtcgt aaccatcatt tttgaatttt
    13921 ttatctaaat tgccattact tttaccgaaa tatttagcgc cgccgcctaa taaaacatca
    13981 actttatgct ttccgttgat tttatcttta taaaattgtt tagcgatttc gtttttatca
    14041 tctctagaag tcacgtgtgc agcatatgct gctggtgttg catctgttaa ttcagctgtt
    14101 gaaacaagac cagtcgactt acctttttct tttgcacgtt caagcaccgt ctttactttc
    14161 tgcttgttac tgtcaacacc gatggcacca ttatatgtct tatgaccaga actaaaggct
    14221 gttccgccag ctgcagaatc agtaatattc tgttttgggt catttgaata tgtacgattt
    14281 gtgcctttta aatatgaatc aaaagcagta ggggtcattt ctttagcatg cggatcattt
    14341 ttataataac gataagctgt gttaaatgat ggacccatgc catcgccaac taaaaagata
    14401 acattttttg gatttttagt attaccaacc gcgaaacttt catctttaga acttttatcg
    14461 gattgcgcaa ttgcaggtgt gacagaacta aaaaccgttg acacgataat aaggttagca
    14521 actgcaaatt ttgtggcttt tttaactgat aacataagac atcctcctga gtatatgact
    14581 atgtcttcag tgtaaaagag gaattttgag caattatgta gttttagtta aaaatatgta
    14641 aacagagtga tttagaataa caaaaaatga atatatatga caatttgtta tagaaagcgt
    14701 tagaatagaa gcgtgtgaaa atatagaatt aaatataatt tgaggtggaa aaatgatact
    14761 agtaatgtta tctccattat taatcatatt ctttatagtg ttgtctattt tagaggagcg
    14821 taaacgtacg aagaaaaagc aactcgagaa agaaaaagca aatacactaa atcaaaatac
    14881 aaatgacacg gaaagttcaa atcaagagcc gtcattgcaa caggctaaag aacaaaaaga
    14941 taacaaagga taattcaatt gaaggaagaa gattatagat gaaaatatta attgttgaag
    15001 atgattttgt tatagcagaa agtttagcat ctgaacttaa aaaatggaat tacggtgtta
    15061 ttgtcgttga acaatttgat gatatactgt ctatctttaa ccaacatcaa cctcagcttg
    15121 tattgctaga tattaatttg ccaacgttaa atggttttca ttggtgtcaa gaaatccgaa
    15181 aaacatctaa tgtgccaatt atatttatta gttcccgtat tgataatatg gaccaaatta
    15241 tggcaataca aatgggggga gatgatttta tcgaaaaacc atttaacttg tcattaacga
    15301 ttgccaaaat tcaagcatta ttgagacgaa cttatgactt gtcagtagct aatgattcat
    15361 tgacggttaa aggttgtaca ttaatattag atgaagcaaa agtcgtgtat caagaacaaa
    15421 acatacagct atctttgact gaattacaaa tattaaagtt attatttcaa aatgaagata
    15481 aatatgtaag tagaactgct ttaattgaaa aatgttggga atcagaaaac ttcatagatg
    15541 ataacacatt agctgttaac atgacgcgcc tgcgtaaaaa attaaatact attggcgtta
    15601 atgattttat tattacaaag aaaaatgtcg gatataaagt atagggtgaa tgcaatgacc
    15661 tttcttaaaa gtattactca ggaaatagca atagtcatag ttatttttgc tttatttggc
    15721 ttaatgtttt acctgtatca tttgccatta gaagcatatt tactagcact tggcgttatt
    15781 ttattattat tactcatatt cataggtatt aaatatttaa gttttgtaaa aactataagc
    15841 caacaacaac aaattgaaaa cttagaaaat gcgttgtatc agcttaaaaa tgaacaaatt
    15901 gaatataaaa atgatgtaga gagctacttt ttaacatggg tacatcaaat gaaaacaccc
    15961 attactgcag cacaactgtt acttgaaaga gatgagccta atgttgtgaa tcgtgttcgt
    16021 caagaggtta ttcaaattga taactataca agtttagcac ttagttattt aaagttatta
    16081 aatgaaactt ctgatatttc tgtcactaaa atttcgatta acaatatcat tcgcccaatt
    16141 attatgaaat attcaataca gtttattgat caaaaaacaa aaatccatta tgaaccttgt
    16201 catcacgaag tattaactga cgttagatgg acctctttac tgatagaaca attaataaat
    16261 aatgcactta agtatgcgag aggtaaagat atatggattg aatttgatga gcaatccaat
    16321 caattatacg taaaagataa tggtatcggt attagtgaag cggacttgcc taaaatattt
    16381 gataagggct attcaggtta taatggccag cgtcaaagta actcaagtgg gattggctta
    16441 tttatcgtaa aacaaatttc aactcacaca aaccatcctg tttcagtcgt atctaaacaa
    16501 aatgagggta caacatttac gattcaattt ccagatgaat aaaaactttc aatattgtaa
    16561 gcatactagt aacatttttt cactaattta aatgttatta gtattttttt gttttaatat
    16621 agaactaaca aagaaatgag gtgcatgcca tgttgctaga agtgaaacat gtaaaaaagg
    16681 tttatggtaa aggtttgaat gctacgacag cacttaatca aatgaattta tcagttggag
    16741 ctggtgaatt tgttgcaatt atgggtgagt ctgggtcagg gaagtctaca ctactaaatt
    16801 taattgcttc ttttgatgga ctaactgaag gtgacattat tgtggatggc gcacatttaa
    16861 ataatatgaa aaataaaagt aaagcactgt atcgtcaaca aatggtaggt tttgtttttc
    16921 aagattttaa tcttttacca acaatgacga ataaagaaaa tataatgatg ccattaattt
    16981 tagctggtgc taaacgaaaa gatatagaac aaagggtaca tcagttggca gtacaattac
    17041 atttagaggg attcttaaac aagtatcctt ctgaaatctc ggggggtcag aagcaacgca
    17101 ttgccattgc acgtgcatta gttactaaac cgacgatttt actagccgat gaacctacag
    17161 gtgcacttga ttcaaaaaca tcaaaggcat tgatgatgtt atttcaagag attcatcaat
    17221 tggaacagac aattttaatg gtaactcatt caaatatcga tgcgtcttat gcagagcgag
    17281 taatttttat taaagatggg cgtctatatc atgaaatata tcgtggcgaa gaaagtcaat
    17341 tagcttttca acaacgaata acagatagct tagcacttgt gaatggagga agtgtcaata
    17401 tatgaagtta agattgttat ggcacatagt gcgacgtcaa tttattacgc agcgacttgt
    17461 aatcattcca ttcattttag cggtaagtgt actattcatg attgaatata cgcttgtgtc
    17521 aattgggtta aatagctaca taaaacagaa gaatgacttc ctagtaccat ttattatcat
    17581 agctaatttt tttatggcgc ttttaacttt tatttttatt ttctatgcaa atcactttat
    17641 gatgtcacaa agacgaaaag agtttagcat ttttatgaca ttgggcatga ccaagaaaag
    17701 tatgcgttta attgtagtaa tggaaactat cttacaattt ataataattt cagtcgttag
    17761 tattgccggc ggatacttac ttggtgcgat atttttcttg tttatacaga aaataatggg
    17821 cagtgaagtt gcgacgttaa ggtattatcc atttgacttt gtagcgatgt ttattacttt
    17881 gattatcatt gctgtattaa tgggcatgct acttatattc aacttgttca gtattaattt
    17941 tcaacggccg ataacttatc aacatcgttc cgattctagt gtcatatcac gatggttgcg
    18001 ttacgtttta attgttatag gaagcgcagc actatattta ggctacttta ttgcattaca
    18061 acaagatacg acgtttggtg ccttttttaa aatatggatt gtcataggat tagttattat
    18121 cggtacttat gcattttttg taggtataag tgaaataatt attagtatat tgcagcaggt
    18181 atcaaaagtt tactatcatc cacggtattt ttttgtggta gttgggatgc gtgtacgtct
    18241 taaaatgaat gcagtcagtc ttgcaacaat cactttgctg tgtacatttt tgattgtaac
    18301 gctcacaatg acattaacaa cctatcgtga tatgaatcat accattacga aattgattac
    18361 gaatgattat gatttgtcat ttagcgacaa ttctaagtca caaatagaac gtcaacaaac
    18421 aattgagaat attaaacgtg atatacaggg ttcggttaat gcaaaagact tcaaagtata
    18481 tgaaacgaca ttatttagag cttctttaga aaagaatcgt gcttattaca agttaaaaca
    18541 ggctagtgat gatattccaa tagacttcat tggtaatgaa ggcgcgatat tttcaagaca
    18601 atcagtaatg attacagctt ttactgcaca agattataat cgatatcacc aaaacaaatt
    18661 acacttagac aatcaaagta taggtatgat tacgaatgta cctattttta aaaagcagtc
    18721 taaggtcggt cttaataatg aagtctttaa agtgaaacaa cttgacgcac atgcgttaaa
    18781 tttgatgatg attcaagata gtatggtttt aattgttaaa gataacaatc aattacaaaa
    18841 agtaaaagga ttttatgcac atgaacaaaa gaattcgaca atatcgataa actttaatgt
    18901 atttggaaag acgaaattaa caacatcaca aatacaaaag ctatcgaaaa aatacgatgc
    18961 gtcaataaat tctaaatcag aaatgctaat ggtttgggat cgtttgacag gtggactaat
    19021 atttgtaggt ggcgtagtat caattgtact tgtcattgga atatttctaa tgatgtacta
    19081 taaacaagtt tcagaagcct atgcaaatca gcacaattat gacattatga aaaaacttgg
    19141 tttagataat ggaagaatag caaagataac acgaaatcaa atgactttct tgttcgcaat
    19201 accaataaca gtagcactga ttcacacatt aatttctagt aacattgtat atacgttact
    19261 aaatatgata ggtattaata accatcatat atttttaaca agttacgtac tagctgtcat
    19321 tataatttcg tttctatata tggcgatgta caaaataact tcctatattt atgctaaagt
    19381 tattcatcaa caacgcaatt aaccatgttt cagtgaaatc acaatcaatc atgtattttc
    19441 actgaaatat attatattaa aaagaaacct aaaaacatta caaataacaa taacaaattt
    19501 caaccgataa gataaaatgt cttgtcggtt tttaatataa caagtgatat tttggaaatg
    19561 ccaaaaatat atagattgtt tatataaaat gatttaaaat gttttataac aacatatttt
    19621 atgcaacaca taaaatgggt aactaacatc acgaaatcat tgtatttgtt gttacactga
    19681 tgaagcgatg tcacaaagaa atgtcgttat caaacgaagc ccgaaataca tactgatact
    19741 cgaaataatg taacgataaa taacaatgat aacgattgat tactacgcaa aaatatatgt
    19801 aaattgacga tttaaatgca attcgtatat tttgccaatg atttacacat taaaatgttt
    19861 gaaaattgtc ataattcagt ctaatttaaa aggaagttaa ttacaaaatt caaaaatcat
    19921 gcatcgtgtc atttagatat tgaaaaagat gagtcaaatt tgtttcatat attttccaat
    19981 aacaaattat tatatctaaa tatacaatca gaaatatatg aatagaaata tcaat
//
LOCUS       DBNENW010000039        19818 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNENW010000039 DBNENW010000000
VERSION     DBNENW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.039"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            208..648
                     /locus_tag="AC1LW8_001087"
     CDS             208..648
                     /locus_tag="AC1LW8_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIP4772936.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            991..1464
                     /locus_tag="AC1LW8_001088"
     CDS             991..1464
                     /locus_tag="AC1LW8_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIP4772937.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1439..2125
                     /gene="saeR"
                     /locus_tag="AC1LW8_001089"
     CDS             1439..2125
                     /gene="saeR"
                     /locus_tag="AC1LW8_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor SaeR"
                     /protein_id="HIP4772938.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2125..3180
                     /gene="saeS"
                     /locus_tag="AC1LW8_001090"
     CDS             2125..3180
                     /gene="saeS"
                     /locus_tag="AC1LW8_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HIP4772939.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3252..4235)
                     /gene="csbB"
                     /locus_tag="AC1LW8_001091"
     CDS             complement(3252..4235)
                     /gene="csbB"
                     /locus_tag="AC1LW8_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HIP4772940.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(4367..5206)
                     /locus_tag="AC1LW8_001092"
     CDS             complement(4367..5206)
                     /locus_tag="AC1LW8_001092"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4772941.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5428..6777)
                     /locus_tag="AC1LW8_001093"
     CDS             complement(5428..6777)
                     /locus_tag="AC1LW8_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIP4772942.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(6998..8179)
                     /gene="nagA"
                     /locus_tag="AC1LW8_001094"
     CDS             complement(6998..8179)
                     /gene="nagA"
                     /locus_tag="AC1LW8_001094"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIP4772943.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8487..10445)
                     /locus_tag="AC1LW8_001095"
     CDS             complement(8487..10445)
                     /locus_tag="AC1LW8_001095"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIP4772944.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GVDSFKPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFAAGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10451..11371)
                     /gene="pfkB"
                     /locus_tag="AC1LW8_001096"
     CDS             complement(10451..11371)
                     /gene="pfkB"
                     /locus_tag="AC1LW8_001096"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIP4772945.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11368..12132)
                     /locus_tag="AC1LW8_001097"
     CDS             complement(11368..12132)
                     /locus_tag="AC1LW8_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP4772946.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12382..12864)
                     /gene="ybaK"
                     /locus_tag="AC1LW8_001098"
     CDS             complement(12382..12864)
                     /gene="ybaK"
                     /locus_tag="AC1LW8_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIP4772947.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13037..13489)
                     /locus_tag="AC1LW8_001099"
     CDS             complement(13037..13489)
                     /locus_tag="AC1LW8_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772948.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENMKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(13782..14948)
                     /gene="norA"
                     /locus_tag="AC1LW8_001100"
     CDS             complement(13782..14948)
                     /gene="norA"
                     /locus_tag="AC1LW8_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HIP4772949.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15157..15579
                     /locus_tag="AC1LW8_001101"
     CDS             15157..15579
                     /locus_tag="AC1LW8_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIP4772950.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            15765..16382
                     /locus_tag="AC1LW8_001102"
     CDS             15765..16382
                     /locus_tag="AC1LW8_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HIP4772951.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            16379..16663
                     /locus_tag="AC1LW8_001103"
     CDS             16379..16663
                     /locus_tag="AC1LW8_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772952.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(16818..18191)
                     /locus_tag="AC1LW8_001104"
     CDS             complement(16818..18191)
                     /locus_tag="AC1LW8_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIP4772953.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(18277..>19818)
                     /locus_tag="AC1LW8_001105"
     CDS             complement(18277..>19818)
                     /locus_tag="AC1LW8_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIP4772954.1"
                     /translation="KHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPSSLPI
                     MAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPKSGSA
                     ILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLSIVGN
                     KTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLIITSV
                     QAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYFIMIK
                     VMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPIDSASI
                     TIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSDQTFG
                     VLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQSIGFV
                     LIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSMTYWK
                     WLGLL"
BASE COUNT         6549 a         3662 c         2781 g         6826 t
ORIGIN      
        1 cagataaata ttttctcacc taaaaaaatg ccctcctctt attttgactc ctatttattt
       61 aaatcagaca attattttca ttttcaaatt attctttctt caatattagt taagcgatat
      121 ttaaacgaag ttaagaatta gttaatggca tattatttgc cttcatttta aacttaactt
      181 atcaaattga agaaatgagg agttagcatg aatacaaaat attttttagc agttggtgct
      241 gttgcctctg tattaacttt aggcgcttgt ggtaattcta attcacaaga tcaaggtaac
      301 aaaactgaac aaaaaacaaa gtcagaagat agcaatgtta aaactgataa aacaaaacac
      361 ctaacaggta cattcagttc taaaaacggt gaaactgttg aaggtaaagc tgagattaaa
      421 aatggtaaat taatgcttac taactacaaa tcatcaaaag gtccagattt atacgtctac
      481 ctaacaaaaa atggcgacat taaaaacggt aaagaaatcg caatggttga ctacgataaa
      541 gaaaaacaaa catttgatct taaaaatgta gatttaagca aatatgatga agtaacaatc
      601 tattgtaaaa aagctcacgt catcttcggc ggcgctaaat taaaataatc aatttctgag
      661 ttaaactttt atttacaaca tactattact atacattaca aattttaaaa atatacatta
      721 cactcattac tcaatggaaa gcgtatgatt tcacagcccc cctagcttgt agaaatcata
      781 ctttcctttt ttcaatatat atacaactat taaatcccat aagattgtag agcacataag
      841 taaaattttt ttagagcttg aggtttgttt agcttaagca acccatgagc tcaaacactt
      901 cctgttcaca taacactaca aatcgcatta tgttgcttaa tcttatgttt atataaatta
      961 cacacaataa atagaaagaa tgtgaacatc atgaataaat tattgctact cgttacattt
     1021 atcattcgtg tgggttcagg tattgttatg ttaatgcaag gctacgaaaa attaacgggc
     1081 ggatttacgc tgaaaggttt agtaccagtc atcgctaaca atactgattc accagagtgg
     1141 tataagtggt ttttcgcaaa tatagttgca catacgacgt cattatttga tattgttgtc
     1201 ccactcggag agattgcaat tggattaggt ttaatttttg gagtttttgc atatgctgct
     1261 agtttctttg gagcctttgt tatgataaat tatatcttag cagatatgat atttacgtat
     1321 cctcttcaat taactttctt tatcctttta ctaatgagtc actcattgtt aaaacagatt
     1381 tcacttaaag aaatcattaa ttactttaga ggtcgtaaga acagaggtga aaaaatagat
     1441 gacccactta ctgatcgtgg atgatgaaca agacattgta gacatttgtc aaacctattt
     1501 tgaatatgaa ggttacaaag taacaacgac aactagcggt aaagaagcaa tttctttact
     1561 atcaaatgat attgatatca tggtacttga tatcatgatg ccagaagtta atggttacga
     1621 cattgtcaaa gaaatgaaaa ggcaaaaatt agatatcccc tttatctatt taactgccaa
     1681 aacacaagaa catgatacca tttacgcctt aactttaggt gcagatgact atgtcaaaaa
     1741 accatttagt ccaagggaac tcgttttacg tattaataat ttacttacaa gaatgaagaa
     1801 ataccatcat caaccagttg aacaactgtc gtttgatgaa ttaacactta ttaacttaag
     1861 taaagttgtg actgtaaatg gtcacgaagt ccctatgcgt attaaggaat ttgagttatt
     1921 gtggtattta gcttctagag aaaatgaagt tatttctaaa tcagaattac ttgaaaaagt
     1981 ttggggatat gactattacg aagatgctaa taccgtgaat gtccatatac accgtattag
     2041 agaaaaatta gaaaaagaga gctttacaac atataccatc acaactgtat ggggattagg
     2101 atataaattt gaaaggagcc gataatggtg ttatcaatta gaagtcaaat cattattggc
     2161 gtcgtatcga gtatactatt aacttcaact attttagcaa ttgcatatat tttaatgtgg
     2221 tttaacggcc atatgacact aactttgacc ttaacgacaa taattacaag ctgtttaacc
     2281 ttattaatat gtagtatttt tattaatcca cttatacaaa aaattaagca gtttaatata
     2341 aaaactaagc aatttgctaa cggaaattac gcaagcaatg ataaaacgtt taattcacca
     2401 aaagaaattt atgaattaaa tcaatctttt aataaaatgg cttctgaaat tacgcaacaa
     2461 atgaatcaaa ttaaatccga acaacaagaa aaaacagaac tgattcaaaa cttagcccat
     2521 gatttaaaaa cacctttagc aagcattatt tcatattctg aaggactacg tgatggtata
     2581 atcacaaagg atcatgagat taaagagtca tacgacatat taattaaaca agcaaacaga
     2641 ttatcaacat tatttgatga tatgactcat attatcactt taaatacagg taaaacatat
     2701 cccccagaat taatacaact agaccaatta cttgtatcaa tattgcaacc atatgagcaa
     2761 cgtatcaaac atgaaaaccg cacattagaa gtgaatttct gtagcgaaat tgatgcattt
     2821 tatcaatatc gaacgccact tgagcgtatt ttaacaaact tacttgataa tgcgctaaaa
     2881 ttttcaaatg ttggtagtcg cattgatatt aatattagtg aaaacaaaga tcaagatact
     2941 atcgacattg ctattagcga tgaaggtatt ggcattatac cagaactaca agaacgtata
     3001 ttcgaacgta cattcagagt agaaaactct cgtaatacaa aaacgggtgg ttctggatta
     3061 ggcttatata tagctaatga actcgcgcaa caaaataacg caaaaatcag tgtaagcagt
     3121 gatatagatg taggaactac gatgactgta acattacaca aattagacat tacgtcataa
     3181 tccgatttat ttataaaata aaatgcaaag actaaaaaga agctcccatt aatgagggct
     3241 tctttttttg tttatttaga ataaacttta tgggtatcct tctcatcatt ttcaatactt
     3301 gaaagatttg tagcttgaat aatatattta ggacgtgcct taacttcata atatatcctg
     3361 ccaatatatt cacctacaac accaattgaa attaactgta tgccgcctaa taataaaata
     3421 gctgcaatcg ttgaaaaata tcccggaata ttaacaccag atatcataat attgatgaat
     3481 aaatagataa tatatagcac gcttattgaa aagataaaca agccaagata aatcatcatt
     3541 ctcaaaggtt tactattaaa ggaaatcaat ccatcaatac cataattaaa taacttttta
     3601 aaggaccact tagattcccc tttttgtctc tcaacatttt gatacgtaaa cactttagta
     3661 ttatagccta tccattcaaa taaccctttt gaaaatcgat tatattcttc aagtgatgca
     3721 atggatttaa cagctctttg gcttaaaagt ctaaaatcac caacaccatc atcaaattgt
     3781 acttcttcta caaagcaatt aactaactta taatacaaat ggcttaatgt tttgcgacta
     3841 aaattttcac cacttctatc acgctttgca atcacttgat catagccttc cataaaacct
     3901 tcaaccattt tcggaatata ttcaggtgga tgttgcaaat cacaatctat catgatgact
     3961 gcatcaaatt cagtactatg ctggtaacct gcaatcatag ctgcttcttt tccaaaattt
     4021 ctactaaaag aaataaagct gacatgccta tctattgtgg caagatgttg catttcatct
     4081 atagtggtat ccgtactacc atcatctatg aaaagcatat tataatcata gcctttcaca
     4141 ctactatctt gtgaaagtat ttcagttaat tgttgatgtg tttgtgtaat gacttcccct
     4201 tcattaaaac aaggaatgac gactctaatt ttcatctatg tccatccttt ttatttaaaa
     4261 gattttgaat gtacctgagt attgttttat caaatagtaa aaaccaactc atttatttta
     4321 atgtacacat taaaaggcat tcataaacgt aaaatttaac atccagttat tttaaatccc
     4381 caattttcac atcatctgga tttttacctt gtcttgcatt tctatttaaa gcatcaattt
     4441 cagctacttc tgttaattct aaattaaaat ctaaaatatc tatattttct tgaatgcgtt
     4501 taggtgtctg agattttgga ataataattc tattgtgtgc taaatgccaa cgtaatacaa
     4561 cttgtgctgg tgttttatga tatttttcag caattttaac aatgacaggg ttgtctagta
     4621 gtcctctatt tctcatcaaa ggcatccatg cagtcacttt aatatcgtga cgatcacaaa
     4681 attcttgtaa ttcttgttgg ttaaaatatg gatgtacctc aatttgattc accattggtt
     4741 tgatacttga ttgagccatt aatttttcta gatgatgaac attaaaatta catacaccta
     4801 ttgcttttac cttaccttgc tcgtaaagtt cttccatagc tttatatgtt tctaaaaata
     4861 gaccatctgc ttcacaaggc caatgtatta gaaataaatc aagataatca gtttgtaaat
     4921 tttcaatcga tttgttgaaa tattcgaatg ttttctcata accttgatag tcattccata
     4981 acttcgttgt tataaacaaa tcttctctat cgacgccatt atcctttaat gctcgtccta
     5041 gtgaagcctc attatcataa aagtatgctg tatcaaacgc tctatagcct gcgtcaattg
     5101 cagcatttac aactttagtc atatcttcgt cagagatttt ataaacacct aatccaactg
     5161 aaggcatcgg gtatccatta tttaatattt gtatctcatt caacatcttt atatctctcc
     5221 aatctatgta tcttttatat ctttacatta ccctaaattt ttcaataaac tcaattaata
     5281 cgaattatcg ctttcaataa aaaatattca ttaaatcatt aaagatattg agttccaata
     5341 ctattttcac ttttcattaa ttttagtcaa aaaaataacc aaccaaaaat gaattaaatc
     5401 attcttagtt ggtttatata ttaatatcta ttctgatttt tcatcttcat cagactgtcc
     5461 gatagtaggt ctcgcttcat taaattcata gtttaatatc acccaaataa tttggtggtt
     5521 atcgatttct gaaacaaccc agcgatcata agttgtatcc acgtaatcat ctttttgtaa
     5581 attggtatta cgagattgta accatccacc tatcgtatca atatcctcag agtcatcaaa
     5641 ttctataccg aactcttcag ttaaatcatc caatagtact ctgccattta cttggaatgt
     5701 cttattatca attttaacga tatcattcac ttcatcatca tcaaattcat cacgaatttc
     5761 tccaacgatt tcttctaaaa tatcttccat cgttaaaata cctgccgttc caccatattc
     5821 atctataata agactcatat gtacatgttc acgttgcatt ctaattaatg catcactgat
     5881 acgtgttgtc tctgaaatca ttggcaactc atgtatatag tttgctattt taatcgtttt
     5941 tccagaagcg tattcagtta aaaattcttt gacgttaata aatcctttaa tgtggtcttt
     6001 atcaccatca tcagtaattg gataacgcgt aaattgatgt tcttttattg tttctagtaa
     6061 ttcgtctaca ttaaaaggtt catttagtgt aatcatttga gttctaggta ccattatatc
     6121 ttttgcatgt ctttcatcga atgaaaagat attttgcata tatgccaatt cagtttggtt
     6181 gatttctcca ccattataac tattgttaat aataattttg atttcttctt ctgacattgc
     6241 atcagtttgg gcatcaggat ttacaccaaa cattctaata ataacacgtg cagaaccatt
     6301 catcagccaa atcaatggtt tcataatgtt accgaaatag aacaatggtc ttgcatatac
     6361 taaagcaagc ttttcagtat gttgaatcgc tatagattta ggcgctaatt caccaagtac
     6421 tacatgcaaa tacgtaacga ttataaatga cactgcaaac gaaatcgtcg tcgttaatgc
     6481 agttggtaaa ttgattgctt caaatattgg gtgtaatagc ttttcaaacg ttggttcacc
     6541 aagccaacct aaccctaaag atgttactgt tatacctaac tgacaagcag aaagataata
     6601 atctagatta gcaatcatct tttttactat tttagcaggt ttatttcctt catctgctag
     6661 ctgttcaatt cttgttgctc taatttttac taatgcaaat tctgaaccaa caaatacagt
     6721 ggttaatgca attaatagaa taaatataat caaactaatt atggtcgaag tttccaatta
     6781 attccctatt tctagggatt cacctccatg ttatatgtgt cactcatggg taacacgcat
     6841 tcaaatttat cactatgact taaattacaa ctacatgtta tcgccttccc atttagacac
     6901 ccccagaaaa aaatgttatt gcttctattt tatcataata taaagtgctt atgttaacag
     6961 attaaatcta ttgcatacat ttaattatga ttatttatta gctaaatgtg tgaaccttac
     7021 cttgttttat tgtagatttt acattcatat catcatctag aataacaaga tctgcatcct
     7081 tatttacttt aatactacct tttctatcat cgatacctaa tgcaatggct tgatttaaac
     7141 ttgttactcg ccataaatga tctaatgtat cacctgtaaa tgatattaag ttacgtaacc
     7201 catgattcat ttttaaaata ctaccagcaa gcgcaccatt tgcaagacgt gcttgttgcg
     7261 attgaacagt tactttttgt ccacccaaat catattctcc ttcaggcata ccttttgcac
     7321 gcattgcatc ggtaattaaa taaaaacgtt cattaccttt catacggtaa gcaatagcaa
     7381 ccgatgccgg atgagaatgt gtgccatcaa caatcatttc ggtatgtaga gcatcattca
     7441 accatgctgc tccaaaaaca cctggttctc tatgctggaa tggcgtcgct gcattatata
     7501 aatgcgtgac atgtttagct cctcgctcaa cagcttcgac tgcttcttcg tatgttgcca
     7561 ctgtatgacc aattgaaaaa ataatgtcat ctttatacgt ttcaagcgct tcttttgcac
     7621 cttcaacttc aggtgcaaac gtcataatct ttattaatct gttagcagtc tcttgaaaat
     7681 gtttaatttt atcgataaat gggcgtacaa cgtattgcgg atgttgagca ccaactttat
     7741 tttcagatat aaatggtcct tctaaatgta tacctacaat ttccgctgca ttgtgaacat
     7801 cttgctccgc ttcatattta gcaatatttg taagtgcttt atctatttta tcagtagatt
     7861 gcgtcattgt agtggccaag tatgatgtcg tcccttcaga taacaaattt tcggatagat
     7921 attttaagcc atcgtatgac ccatccattg catcttgacc ataaccacca tgaatatgta
     7981 tatcaataaa acctggtaat acatgatgac ctttagcatc aatcacttga attttatttg
     8041 tcgtatcatt atcaattgct gctttatcat ccccttctcc aattgcaaca atctgtccat
     8101 ctttcacatg aatgtaacca ttatcgattt tgccatcttc agtataaact ttgccgttat
     8161 atataattaa ttctgacacg tataaaccct tctttattta taaattttca ctttaagaca
     8221 ctcatatttt actatatctg cttcgtttgg cataactttt tgcaaaatat taaaaaagtc
     8281 tatacatttg tggtgtctat ttcttcattc atttatctaa aaataaaaac accttcatat
     8341 caattcataa aaactgaatt aatagaaagt gtttttacag ctttgtataa gctaccgtta
     8401 acacgatgta taaaactaac atatattcat tgaacttaca acttaatatg aagctctttg
     8461 ctaacaatca ttttacatca ttaaaactac tcgtccattg attttgaagc ttcgatttct
     8521 gtttcagtta acttgggttt gattaaaccg taaattaatg ctgaaactaa tgtaccaact
     8581 agaagtgcaa taagagtttg aagtaagtgt gcaccatccg taccaacaat tacaataata
     8641 ccaccatgtg gcgcagtaat tcgtgaacct aagcctaaag caattgcgcc acctatacct
     8701 gaaccaatca tcattgaagg aataacacgt aatggatcgg cagctgcaaa tggaatcgca
     8761 ccttctgtaa taaaggacat acccatcaca tagttaggga taattgaacc acgttgttct
     8821 tttgtaaatt tacgtctgaa aattaacatc gctgtcgcaa ttgctaacgg tggaatcata
     8881 ccaccaatca ttgcagctgt aattggtgct gcattacctt caatcaacgc acctgttgca
     8941 aaaacatatg ccgctttgtt gaatggaccg cccatatcaa ttgccatcat agcgccaata
     9001 actaaaccta ataatacaat attagaacct gataaattgt ttaatccatc taataacaaa
     9061 tgatttaacc aagatgctgg tggattaaag acataaatca tcaataagcc tgtagctgtc
     9121 actgtcaata gtgggtaaat taatgttggt tttaatccct ctaatgcttg tggtaactta
     9181 cgtgtcatcg ctttaacacc ttgtgttaag taacctgcta agaaacctgc aataatacca
     9241 ccaataaatc ctgaaccacc tgaaattgct aacataccac ctacaagacc agctgcaaaa
     9301 ccaggtttat ccgcaatact acgtgcaatg aatccagata aaattggaat gattaacgcg
     9361 aatgcacttt tactaccaat gttccatagc tgctctgcaa acgcattgta ctctgagctt
     9421 tttggtttaa atgagtctac tccaaataaa aatacgattg ccattaaaat accaccagat
     9481 attacaagtg gcaacatgtt agaaacaccg ttcattaagt gtttatagaa tgctttacct
     9541 gggcttaatt tttcttgact gtcatttgag ttaccttttc tttgaccatc acgggcaaca
     9601 aaaggtttac gacttgtatc taatgcttta ttaattaatt cttctgggcg tttaatacca
     9661 tctgctactg gtacttctac gacattttta ccatcgaagc gatccgtctc aacatgaaca
     9721 tcagcagcaa caatgatacc tgttgcattt tcaatatctt gttcagttaa atggttttta
     9781 atgccgcttg aaccattcgt ttctacttta attttaatac ccatcttatc cgcttgtttt
     9841 ttcaatgcgt cacgtgccat gtacgtatgt gcaatacctg ttggacaagc agttactgct
     9901 aacacatatg gctcattaga ttgtttagat gattgttctg cagttgcaac ttgttgtgct
     9961 tcagcttctg cctcttcttc ttttgtcact tcatcatcag cctcatctat gatcgctagt
    10021 acttcttcag gtgatgaagc atgtaataat ttctcacgta cattttcatc cattaaaata
    10081 ccagacaact tagctaaagc atctagatgt gtttgggcgc caccttctgg cgctgcaatc
    10141 ataaagaata agtgtgctgg ttgcatatcc aaactttgat aatctacgcc tgctttagat
    10201 ttaccaaacg caatagctgg tgacttaact gcggccactt tggcatgtgg aatggcaata
    10261 ccttcgccga taccagttgt actttgtgat tctcgattgt gaatcgcttc cttaaatgac
    10321 gcgacatcac ttaatttacc tgctttgtct aattgattta ctaactcatc aataacacca
    10381 tttttgtcat ttgccattaa atccattgct attgtatctt ttgttaataa ctctgttact
    10441 ctcattattt tcactcccca tcaagtacgc taatcgtaac ttgtgatttt attttttcta
    10501 tagcgtcccg tgttgctaag tcctcatcaa atgccgtggc agtaccgcat gcgactgctt
    10561 gttggaatgc tttttcaatc gttaaacctg aagcaattcc agccaccatg cctgcaactg
    10621 tactatcacc agagccaact gtattaacca ctttcccttg tggattaact gctttaatac
    10681 tgatttcttt atcaatataa atagcaccat caccgccaag cgagacaata acagattgcg
    10741 cacctttatc aactaacaaa cgaccatatt taataacatc tgtgtctgag ttcactgttg
    10801 tattaaacat cacttctaat tcatctttat taggtttaat aaatagtgga tgatatggta
    10861 aaacgctttc agccaattct ttttcagcgt cgactactaa tttagcacct gtctgtgctg
    10921 taatttgtgc aatttgcgca tacgcatcgc ttggaatact acttggtaca cttccagcaa
    10981 caataactat atcttcgctt gttgtatttt taatttgttg taacagttgt tcaaattgtg
    11041 ttgacgttat atgaggaccc ggtgcattga tttctgtttc ttgtcctgtt tttaatttca
    11101 cattaatacg tgtatcttca tcaacttcaa taaaattcga ttgaattgca ctgttattta
    11161 atgtatctat aatgaatttc ccaggaaatc cacctgcaaa tcccaaggca gttgactcaa
    11221 catccaatgt ctttaagacg cgcgagacat taataccttt ccccccagcg aatttatatg
    11281 ttgctgttgc tctgttcaaa ccatcaattt taaaatcatt cgtaaaaatg acatagtcaa
    11341 ttgaaggatt gaaagtcact gtataaatca taaagtccct cctataaagt gatacttttg
    11401 ttggtattct tttaacgatt cttgatttaa tgctttttca gatgtgatga ttgtcgtact
    11461 ttctagcaaa ggtacacgag caaaatatac tttattaaac ttagaatgat ctataagtac
    11521 aaatgattga ttggctaatg acattgctgt ttgtttaact aatgcctctt gctcatcggg
    11581 agtagttaat ccaagttcaa tatctaatcc attcatcccg ataaaagctt tatcgaaaca
    11641 atatcgtctt aatatctcca tagcactaga accaatcgta gcaagtgtat tttctttaac
    11701 ttgaccacct agcataattg ttttaatacc ttttttaagt aaagcttcta catgtgttaa
    11761 accattggtt accacaatga tatctttcgc ttgaatatat ttaattagct ccaatgtaga
    11821 tgaaccagca tcgataaata agcattcatt atcgttgatt tgattagctg ctattttagc
    11881 aatcattttc ttttcatcaa gattcgttgc taatttttca gttaaattcg cctcaaccat
    11941 acgattttct tttaacattg caccaccatg cacacgttgc aatttcccta attgttgtag
    12001 tttagataaa tctcttcgta ttgttgaagc actgcatcca gttcgatcta ttagttcttg
    12061 taaagtcaaa aaatctttgt gcgaaagttc ttctaatatt aactcgtgtc ttttttctgt
    12121 aattatcatc atcaccccat ctcactctgt attataatga gtacgctttc acttttcaat
    12181 cataaattac caaaaacaat cacaaacaat caaattgtga cgataaaatt acatttttat
    12241 gttcaaatac tcatttgtaa tgactgttta tgatcgtaaa atgctaattc aatcaaaaag
    12301 gagtgtggca gaaatgaatt ttataaaaat ttcatctctg tcacactccg aatcttgccc
    12361 catacttttt gttgttatta atcattcatg gataactgct ccaattttgc ctttagttat
    12421 tgtaatcaaa tcctcaacag ctattgtgat ttgcattgtt cgaagcccac cactcacact
    12481 gatatgacta taattttcac acgatttgtc aacgactgtt ggaaacaatg ttttcatacc
    12541 aacaggcgaa cacccaccac gaatgtatcc cgttacattt ttcaaattat ctaaaggcat
    12601 aagctgcaat ttcttctctc caaccaaagc agctgccttt ttcatatcta aagtttcact
    12661 tactgggata acaaatacaa aatgttcatg ttttgtattt tctagaacaa gcgttttaaa
    12721 tactgtttta acatttgctt gtatgagttc tgcgacttct tgaccagata aatgttcttc
    12781 tggtacctca aagctatgaa cttcgtattt aatttttgca cgatcaagca tacgcatcgc
    12841 gttcgttttc ttatttttag ccatttcaac acctcattta tacttcttca gaactatatt
    12901 aacattttaa aatacattac ggattggttg attttgacat aacatttaca tcattttata
    12961 agttgatttc acctttttaa attaaattat tgcaaaataa aaacagtata tccaattaga
    13021 atacactgtt ccattattat ttcgttatta tgccttgatt tgaaatactt acatgttgct
    13081 tactagttga attatcattg gaataaacat aattatatcc atgatttgtc ttagaaattc
    13141 tttctaaacc ataattatca ttgatatgct tatctgcata acgatcagca ataacttggc
    13201 actcttgttc gttaaaagaa tttgatttat ttgcattcac acgatgttta aaatgatttt
    13261 gctttttcat attttcattc acttcagatg ctttagcagt atgaatattg agaatatcca
    13321 ttttcttttt ataagtatct tcagttgata catttatcgc gttaagcgta ttaattgttt
    13381 tatgtacctt agcaagcgca tcttcatttg ttgctaatat taataaacca attaaacaaa
    13441 cggttaatat tacagcaaca aagcctaaaa ataaattttt tactgtcatc cccttaccca
    13501 catttcctta ctcatttttt atcatcttaa cacattaacg ttaatgaatt aggtatgtgg
    13561 attgcaattt aaaaagaatg taatatttgt aacatgttta ttactcaaaa tcattttttg
    13621 ttacatataa catttaaata taaagagtaa aacatttagt caatcgaatc tttttaaatg
    13681 taaagcatcg aattttctta ataaaattca taagaaaaac gatgctaatc attcataata
    13741 ttacgcttta ctttatatca atatacacca aaatacttat gctacatatt ttgttctttc
    13801 aattttgctc tatgttgctt ttcaattaaa acaataacaa cacctgctaa tgaaacacct
    13861 atagccatat aaattggtgc ttcaatgtgt acatcaaata acgcacctgc gattaaagga
    13921 cctatgaaat tacccatact agtgaatgtc gagttcaatc cgcctgcaaa gccttgcctt
    13981 tctccagcaa tattagaaaa ataatttgta atggctggtc gtatcatatc aaaacctatg
    14041 aagacaacaa aactgattaa cattattgac caatagccat tagcaaaaac taataatatt
    14101 aagacaacaa ctgaatataa taatgaccaa gctataaatg ttaactctga gaaatacttc
    14161 ataaatttat cgaagaaata gatttggaaa agtgccccaa atataccgcc acccgtaata
    14221 gcaatcgaaa tatctttagg tgaataattt accttgtcag ctgtgtatag tgaatacaat
    14281 gtttcaaatg cagataaacc aaacgataat acaagtgtta aaataactgg tgtaataaac
    14341 actttccagt taattttcgt tagcaattgt ggctctaact tttggaaacc acttgtcgta
    14401 gactttttcg gatcgtgaat caatacaatt gacattataa atgctagaat acctaatgct
    14461 cctgcaaagt aaaatggcat acgatgtgaa acttctgcca taaatccacc aatccctggt
    14521 cctaaaatga atccagaatt gataatcgct gacatgtagc caaagttttt tgctttttga
    14581 tggcttggtg aaatgtcagc tattaaacct gtcacaccag gcattaccat accagcactc
    14641 ataccaccaa tcactctcga taacatcaat accgaaaaat tgtggccaac tgcaaacata
    14701 aattctgaca ctgaaaacaa aattaatcct atacatataa ttaatttctt ccctaatttg
    14761 tcagctagcg taccaccaaa cggcgatata atcatttgag ataacgcaaa agcagcaact
    14821 agtaatccta aatcactacc agttaatccc aaatctttta aataaacagg caagactggt
    14881 attactaaac cgatacctaa aaaaatcaag aaaatattaa aatataagac aaaaatctgt
    14941 ttattcatat gctcacctct tttttctcca tgtcatgctt aaagctgtta ttcttctttt
    15001 ccacgacaga ttgcaattca tgctaaatat tcgtaaaaag tttctatatt gtattacaac
    15061 attgctacat ttgacaatat tttttctttg taattatcac tattttccat ttaattgtat
    15121 aattaatgac atattaaaat aaaggggatt gatcatatga aattgcaaaa aagtaatcat
    15181 actatactac tagtgttgga aaaaggagaa gatatcgttg aatgtataac aacctttgca
    15241 gatgaccaag acttaacatt tacatctgtc agcggcattg gtgcatgtga tgacgttgta
    15301 ttaaaatttt tcaatttaac aacgaaacaa tacgaagaaa aacatattac tgagccactg
    15361 gaattgacga gcttattagg taatatttct cgtctagaca atggtcattt tgcacatttg
    15421 catgctactt tcggtactca atcatatgaa acttttagcg gacatctcgc taaagcaatt
    15481 gtttctgcaa ctgctgaaat cattcttact gtaactgatt tggacattca acgttcattc
    15541 aaagatgcag tcggtttaaa tttacttgat cctcaatagc actttacttt ttagttgaat
    15601 caaattttat tacaagttat cataagtgct taatcataaa tgcattctcg tttattaata
    15661 tacttcaacg agttaaatta aatttttaat taggaattga atgcgtttat aactatcaca
    15721 tttgaatgaa taactttttc attagaaagt agtgtttatt attgatgcaa tctcgataca
    15781 tcgctagaat ttacttttta attttattta tcgtctccct ttttgagact aaaatttttc
    15841 aatttatgac tttaaatttg tttttagcat atataccttt tgagcttagt ctattattac
    15901 gattattcaa accgaataaa aagtatgaat ggccactctt tatcatatat agttttatat
    15961 ttgtgttact tttaccaaat acattttata tggtaactga tttaatccat ttgaaccaat
    16021 ttcaattcaa cttttacgca ggactaaatt taagtgagtg gaaatacttt acctatttac
    16081 tgcttggtgt cttcttagcg gtttatgtga tgattttaat ctattttgaa atactaacat
    16141 tgacttctca ccgttggctt aatagattaa ttgttgtcgt gctaatgttt ttaaatggat
    16201 tcggaattta tattggtcgt ttcttacgct tacattccgt ttatttcttt aatgagccct
    16261 tacatgttct aaatcacatt ctagaaagtc taacgcttaa aactgctatg tttgtatgtt
    16321 ttatggttgt catgcaagca gcaattctat tatttggaaa aggagtgcgt ttgaacaagt
    16381 gacaataaat ataatcgttt taatagtatc tatcattgtc tttcaattga ttattggaca
    16441 catatggcat gatattggtt taagttattt aagaagtatc ttactcatga tgctgccatt
    16501 tggactaggt gtatttatac aacaagtgtc gtattacgaa cgacaatatc ctaaatggca
    16561 agtgcctcaa aacataaaag ttagattgaa atatatttat ctcgctacgt ttttagagta
    16621 tgtcgtacta tacctcactt tatttacgga catactaaga taacatcaaa ttaataagaa
    16681 aactaaatac aacttatata tttgaggaaa gccaatttct atcacatcaa taatagaaat
    16741 tggcttattt atattgctta agaactttat attctgctcc agattttact catgccagat
    16801 ctatcaattg agccgaatta gtctagcgct ttaaatgtag ctaaaacttg tgtacgtttt
    16861 tcttgatgat ctaccatttg tcttggataa tgactaccta attcaatccc ctgttcaaaa
    16921 aggttggact cattgcgttg tgtatcatgc aaatattttg catcaatttg attaaaaatc
    16981 ggaagatatg ttttgatata caaagcttta gcatcaaagc gttcactctg tcttattgga
    17041 ttaaacattc taaaatacgg cactgcatcc gtacctgtag aagcagacca ttgccatcca
    17101 tgaatatttg atgctgcatc atagtcaata aggtgctttc taaagaattt ttctccccat
    17161 gtccaatcta taaataaatc tttggttaaa aattgcgaca caaccattct cattcgatta
    17221 tgcataaaac cagtttgtgt caattccatt attgctgcat caatgattgg aaatcctgtt
    17281 tgcccttcgc accatgcatt aaaatccgct tcattttgcg accattttat ctgtcgatat
    17341 ttaggtttga aagcttggta tgaggtttca ggatactgtg tcattaacac ataataaaat
    17401 tctctaaaaa tgagttcacg tataaatgct tcatagtttg cctcatcttc atcataacgc
    17461 gccaataaat cattaaaaat ttcaataata tctaataatc catatgctaa agcaacactt
    17521 agctgacttg ttaatacttc aggtaaatag tctcttccgc tttcgtaatt ctgtatatct
    17581 tgatctaaaa aatgttgcca tttattctgt tcaattattt gtgaggatcc aaatgctatg
    17641 tcatcaaaca ctaaatcatc aggtgatgca atgacaaagt cttcgaatga ttttaaatca
    17701 tagtgatata cgtctctatg cctcaagtaa ggtcgccatt ttttatagaa actagtaaaa
    17761 actttgtatg gctccccttg ttgattcatt gttttactcg ctttaaagta atgattccca
    17821 cgtatcaaag taacggcaat gcgtgcttca ttgaataaag aacgttgatg taaaatatca
    17881 tattcttcaa gatgataact cataatatca cccgcagtca ctacttcaga gatattgttt
    17941 tctttacaaa atgaaatgat atcgtcataa gacatgacat aaggtgtgag cttttctgcg
    18001 tgcaacgatt ggtaaaagcg ctgcaaagta ccgcgataaa actgtgcttt caaatcagaa
    18061 gctgaagaca tgtcctcttc cggaatgata aaataacatg catcaatgtc ttctttttgt
    18121 tgataaatat attcaaataa tggattgtgt tccattcgaa acattcgatt taataacaca
    18181 gcaattgcca taaacaaaac acccttcaat tatcttttta ttttattaag ctttttgtca
    18241 ctttaaaata acctagattc ttatttatat ttttaattaa agcaaaccta accatttcca
    18301 ataagtcatg ctaaaaacta tcactagaat ataccctaca attgtcaatg gtatacctgc
    18361 cttcaagaaa tctttaaccg taaaagtacc agtgccataa gccaacatat tttgaggtgc
    18421 actaacaggt aataagaaac caaaactaat aacaaattgt tgaattaaaa caaatcctat
    18481 agactggtct cctaagtgta acgtagaggt tagcgaaata aaaacaggta ttaacgctga
    18541 tgataaactt gttgcactcg caaagcccaa atgaatcaat atattaaaaa gcgtgataag
    18601 tgcaattgtc gcgataatag gtaaatgttt taaacctaaa acaccaaaag tttgatcact
    18661 taaccattga gctgcacctg ttttcaaaag aacgttccct agtgaaatac ctacaccaaa
    18721 cacgataatt gttccccatg gtattttatt ttcaacatgt ttccatgtca tgacaccaat
    18781 tttcggcatt aacataacac ctaaagcaat aatagtaatg gatgcagagt caatcggatg
    18841 taatactttt tcagttgacc aaaacagtaa taataacatc gatataacaa ttaaacgcca
    18901 ttcacgtggg ctaacggggc caagtttatg caattcttct tttattaaat ctttaccacc
    18961 ttctattgta ttaatttctg gaggcatcac tttaatcatg atgaaatata aagctacgga
    19021 cataactatg gaccaaggcg ctgcatataa gaaccactcg ccccatgaaa catcaaatcc
    19081 taattgatgg tttataaaat taatcgctac gatattttgt gctgccgccg ttttgatacc
    19141 aatattccaa attgacacag cttgtactga agtaattatt aataaagacg ctaacttgct
    19201 atctttggaa actttaaatg ccgcaatcat acccagcaag attggtacaa ctgcccctgc
    19261 tctagctgtt gcagaaggaa cgaaaaatgc aagtacaatt gaaacgataa ttgctccaat
    19321 aactatattt ctagttttat taccaacaat tgataacact aaaagagcta gtcttttatg
    19381 caaattcgtt tcttgcatag cagcagccaa aaataatgca gctgcaacga gagctacagc
    19441 tgaagttgca tatccactaa acgctaatga taatgcatga ttagttccta gaaggtcact
    19501 tccagctaaa atagcactgc cacttttcgg attacctagc ttctccccta aattttgaac
    19561 cgggctaaat cctaaaagta atatcattaa gccaataatt aaagttgctg acaccggata
    19621 tgatacagct tccgttaccc acataataac tgcaaaagct aaaatagcta gtactgcctt
    19681 agccattatc ggcagacttg aaggtgccgg gagtaatagt acaactataa gtattataaa
    19741 actcatgaca atccaaaatg gcttgtattg cttttttgtc ttttctttct tttgttgttc
    19801 atgttccact acatgttt
//
LOCUS       DBNENW010000040        19659 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNENW010000040 DBNENW010000000
VERSION     DBNENW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.040"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(14..181)
                     /locus_tag="AC1LW8_001106"
     CDS             complement(14..181)
                     /locus_tag="AC1LW8_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772955.1"
                     /translation="MKRIVILENVIVDDPIRLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNSIHI"
     gene            complement(309..812)
                     /locus_tag="AC1LW8_001107"
     CDS             complement(309..812)
                     /locus_tag="AC1LW8_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HIP4772956.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(805..1392)
                     /locus_tag="AC1LW8_001108"
     CDS             complement(805..1392)
                     /locus_tag="AC1LW8_001108"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HIP4772957.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1404..2204)
                     /gene="racE"
                     /locus_tag="AC1LW8_001109"
     CDS             complement(1404..2204)
                     /gene="racE"
                     /locus_tag="AC1LW8_001109"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIP4772958.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2440..3255)
                     /gene="sdhB"
                     /locus_tag="AC1LW8_001110"
     CDS             complement(2440..3255)
                     /gene="sdhB"
                     /locus_tag="AC1LW8_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIP4772959.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3255..5021)
                     /gene="sdhA"
                     /locus_tag="AC1LW8_001111"
     CDS             complement(3255..5021)
                     /gene="sdhA"
                     /locus_tag="AC1LW8_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIP4772960.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5073..5687)
                     /locus_tag="AC1LW8_001112"
     CDS             complement(5073..5687)
                     /locus_tag="AC1LW8_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HIP4772961.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6011..7792)
                     /gene="uvrC"
                     /locus_tag="AC1LW8_001113"
     CDS             complement(6011..7792)
                     /gene="uvrC"
                     /locus_tag="AC1LW8_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIP4772962.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFIDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7958..8053
                     /locus_tag="AC1LW8_001114"
     CDS             7958..8053
                     /locus_tag="AC1LW8_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772963.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8116..8430)
                     /gene="trxA"
                     /locus_tag="AC1LW8_001115"
     CDS             complement(8116..8430)
                     /gene="trxA"
                     /locus_tag="AC1LW8_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIP4772964.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8603..10951)
                     /locus_tag="AC1LW8_001116"
     CDS             complement(8603..10951)
                     /locus_tag="AC1LW8_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIP4772965.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSVKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10961..12673)
                     /gene="polX"
                     /locus_tag="AC1LW8_001117"
     CDS             complement(10961..12673)
                     /gene="polX"
                     /locus_tag="AC1LW8_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIP4772966.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILLDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12746..13267)
                     /locus_tag="AC1LW8_001118"
     CDS             complement(12746..13267)
                     /locus_tag="AC1LW8_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIP4772967.1"
                     /translation="MVIDFIIIIFFVYFVMVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13275..13534)
                     /gene="zapA"
                     /locus_tag="AC1LW8_001119"
                     /pseudo
     CDS             complement(13275..13534)
                     /gene="zapA"
                     /locus_tag="AC1LW8_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
     gene            13904..14842
                     /gene="rnhC"
                     /locus_tag="AC1LW8_001120"
     CDS             13904..14842
                     /gene="rnhC"
                     /locus_tag="AC1LW8_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIP4772968.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYNHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSHLDTISKKHFKNREKAQKILKPL"
     gene            complement(15077..17479)
                     /gene="pheT"
                     /locus_tag="AC1LW8_001121"
     CDS             complement(15077..17479)
                     /gene="pheT"
                     /locus_tag="AC1LW8_001121"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIP4772969.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKDKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPDTTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMSVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17479..18537)
                     /gene="pheS"
                     /locus_tag="AC1LW8_001122"
     CDS             complement(17479..18537)
                     /gene="pheS"
                     /locus_tag="AC1LW8_001122"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIP4772970.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(18918..19658)
                     /locus_tag="AC1LW8_001123"
     CDS             complement(18918..19658)
                     /locus_tag="AC1LW8_001123"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIP4772971.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         6084 a         3794 c         2746 g         7035 t
ORIGIN      
        1 tttagaaatc cttttaaata tgtatactat tcatgatttt aactaaaatt tcaaatttcc
       61 tgacttcttg gaatcttaat tttatacttt ttaataatac acttttaacc aaataatcaa
      121 catgattcaa ccttataggg tcatcaacaa tcacattctc caaaattaca attcttttca
      181 taacataaat gtgttattca ttccaattat cttataaaat tgtcagaaat acttttaaac
      241 aaacattcat gacacgacct cctagttaaa tcagtctata aactaaaaaa agccatagct
      301 acttctattt aaaaagtagt atgactgatt gtttcgtgat gtcgattttt gaaattgatg
      361 gtgccatgta aagtttgatc atcaataata acttcagcat atgtttcttc cattgaactt
      421 ctagattgag atatacttcc aggattaata acatgaacac cattaatata ctcatatttt
      481 gccacatgtg tatgtccata aaatgcaaac aaacaaccta attcaagtcc tttttcagct
      541 aataaatctc ttgttcgatt gacttgatat aaatgtccat gagtataaaa tgctttcacg
      601 tcattatatt ttgcgaccgc ttcattttca aattctggat aaaaatcaca attgcccttt
      661 actctattaa ataagctaag ttccgtatca tcatacgcga attctgaatc tcctaaatgt
      721 aaatatacat ctgcatctgg gtgcatttca taaatttgat ataaaacgcc tgattcagta
      781 tggttatcac tcacaataat ccatttagac atttttatca ccttcaagaa aagcttgaag
      841 taaattaatc gcatttcgtc tatgactaat ttgccctttt tgttcttttg aaagttgagc
      901 catggtttta tctaatttcg gtacataaaa tatcggatca tatccgaaac cattttcgcc
      961 atattttcca tctgcaattt cacctgaaac agtaccttta aatacttttg tttccatatc
     1021 agggccactc atacttatga cacaaacaaa ttgcgcacga cgatcagttg tattaccaag
     1081 cttatttaat aatttttcaa tatttgcttc atcgctttta ttttcaccag cataacgtgc
     1141 agagtatata cctggctcac catttaatgc aaaaacttct agtccactgt catcagctat
     1201 gactgtttta ttcaatgctt ttgcagcagc ttctgatttt aatatagcat tttcttcaaa
     1261 tgttgatcct gtttcttcca catcaaaatc tggtattagt tctgaaatac ctattacgtg
     1321 gtaatctgga aatattactt taaagtcatt tattttccct tgattattcg atgcaataac
     1381 aatctctttc attaaaaatc ctcctagtca ttcactgata tacgttccac attgacagat
     1441 aaatttaacc actctttgat aatgttagta atgtgagtag gatcacctgt tgcaaaaaat
     1501 cgatgatctg gatgttcagt ataacttgca tgttcattac taaatgttag caatgcacta
     1561 acttcacgag ccgtttctaa tccagacgaa atcactgtct ttttaccacc aaaataatca
     1621 tagataggtt tatagagcaa tggatagtgg gtacatccta aaatgacagt atcagactca
     1681 ctattacgcc aacgtttcag tgtttgatga atgacaatgc ttgtaattgt tggatcacta
     1741 tatctcattt gttctacaag tggcacaaaa cctggacagg caacgccatg tacctctaca
     1801 tgtggattga tacgtttaat atgcgtacga tatgcttcag atttaattgt gccttccgtt
     1861 cctagtacta atacattttg atttctagta gtcattattg ctgttctagc acctggttca
     1921 attacgccaa tcactgggat tgataaggtc ttttgtaaat attctaaagc tacagcagtt
     1981 gctgtattac aagcaatcac gagcattttt atatcaaatt ccattaattt acgagcgatt
     2041 tcaactgtat attgttttac ttgttctcct ggtcttggcc catatggaca tcgtccaata
     2101 tcacctaagt aataaatcgt ctcatttggc aactgacgca taatttcttt agctactgtc
     2161 aaacctccga caccagagtc tattacacct attggtttat tcatattcgg tcatccttac
     2221 ttttaattta atttttagtt tatcataact aagcattgga ttttagtatt atgcactgtg
     2281 tttaccattt ttgtcattat aatatttatt ttaaatcagc ccactatcat attgtcatgt
     2341 aatctgctta ttaaaaaaat cccttccaag ttattgtgta tctccattca atttaatttt
     2401 gaaaggaaca taacatttta actcaaaagg gattaatttt tattctactt catggtctga
     2461 accaaagaat gatttaaaca tgtggaatgt tgtttctctg ttcattgctg caatggatgt
     2521 tgttaatgga atacctttag ggcaagcatt aacacagttt tgtgaattac cacactgctg
     2581 taagccacca gtacccatta atgcatttaa acgttcatct ttagtcatag atcctgttgg
     2641 gtgcaaatta aacaaacgaa cttgcgagat tgcttgcgca ccaacgaatt tattattttc
     2701 agtaacatta ggacaaacct ctaaacatac accacatgtc atacatttag ataattcata
     2761 agctgtttga cgttttttct ctggcatacg tggtcccgga cctaaatcat acgttccatc
     2821 aattgggatc catgctttca tacgttttaa gttatcgaac attctagaac gatcaacttg
     2881 taagtcacgg ataactggga aagtattcat tggctctaaa cgaataggtt gttctaattg
     2941 atcaacaatc gcagaacaag attgtcttgc acgaccattg ataaccatag aacatgctcc
     3001 acatacttct tctaagcagt tcatatccca gacaacaggt gttgtttttt cacctttaat
     3061 attaactggg ttacgtctaa tttccattaa acaagcaatg acgtttaaat tttcacgata
     3121 tggaatttca aatgtttctt cataaggctt agaatcactt gtatcttgtc gtttaataat
     3181 taattttact gttttttgtt tcggtttaga ttgtgtttca tgttgtggag tgtttttcac
     3241 tgattgttca gtcattattt tttaccccct ttagacttac ttgtgtaatc acgtttacga
     3301 ggtggtatta aactcacatc gacgtcatca taagtaaact gtggtttttc aaatgcgcct
     3361 tggaatgagg ccattgtcgt ttttaaccac tcttcatcat tacgctctgg gaattctggt
     3421 ttataatggg caccgcgtga ttcgttacgg ttatatgcac caatcgtaat aacacgtgca
     3481 agtactaaca tgttccatag ttgacgggta aagaataccg cttggttact ccaagtttga
     3541 gtatcttcca tatcaatatc ttcataacgt ttcatcaatt caacaatctt tttatctgtt
     3601 tctaacagtt tttcattttc acgaacaaca gttacatttg ctgtcataat ttcaccaagt
     3661 tcacggtgta atttataggc attttctgta ccgcgcatag ctaataattt atcaaaacgt
     3721 tcttgctctt cagctttacg tttttcaaaa atactttcgt ccatatcagt atatgatcga
     3781 tcaatatttg aaatataatc aatcgcgttt ggacctgcta ctgtaccacc ataaatcgct
     3841 gataacaatg aattggcacc taagcggtta ccaccatgtt gagagaagtc acattctcca
     3901 gctgcaaata accctttaat atttgtcatt tgatcataat ctacatatag accacccatt
     3961 gaatagtgaa cagctgggaa aatcttcatt ggtactttgc gtgggtcatc accagtgaat
     4021 ttttcataaa tctcaatgat accacctagt tttacatcta actcatgtgg atctttatgt
     4081 gacaaatcaa gatataccat gttttcgcca tttataccta atttttggtt aatacataca
     4141 tcgaaaattt cacgcgttgc gatatcacga ggtactaagt taccataatc aggatatttc
     4201 tcttctaaga agtaccaagg cttaccatct ttatatgtcc aaattcgtcc accttcacca
     4261 cgtgctgatt cactcattag tcgcagttta tcatcaccag ggattgcagt aggatgaatt
     4321 tgaatgaact caccattagc ataaatagcg ccttgttggt aaacaatgga agccgctgat
     4381 cctgtattaa tcattgagtt tgttgtttta ccgaaaataa taccagggcc acccgttgcc
     4441 ataataactg catctgaacc aaatgtttca atctcagcag ttgtcatatt ttgtgcaacg
     4501 atacctcttg cactatcatc gtcaccttta actatgccaa ggaattccca tccttcatac
     4561 ttcgtaacta atccatctac ttcatatgca cgaacttgtt catccaatgc atataataat
     4621 tgttgtccag ttgttgcccc tgcatatgct gttctgtgat gtaatgtacc accgaaacgt
     4681 ctaaaatcta atagaccttc atttgttcta ttgaacatta cgcccatacg gtctaataaa
     4741 tgaataattt taggtgctgc ctctgtcatc gctttaacag gtggttggtt tgcaaggaag
     4801 tcgccaccat acactgtatc atcaaagtga atccaaggag aatcgccttc ccctttagta
     4861 ttgaccgcac cattaatgcc accttgggca caaacagagt gcgaacgctt tactggtaca
     4921 actgagaaca aatctacatg tgcacctttt tctgccgctt taattgttga cattaagccc
     4981 gctaggccac ctccgacaac aataagatgt ttctctgcca taaaaatttc actcccctaa
     5041 attttcaatc tatatttgtt aaatgcgatg tattacataa aggcaataat tgcagtaaca
     5101 ccaatatacg aaataactaa aaatacgatt aatgaaaccc atgtaaatac tcgttgtgat
     5161 tttggagatt gaagtccacc ccaagtaact aagaatgacc ataagccatt tgcaaagtgg
     5221 aacacaacag caataataca aataatataa aatattgccc atccaggatg ttgcaatgtt
     5281 tcgtgcatta aatcgtaatt cacttctttg ccgtaaaatg ctttttgtaa acgtgtttgc
     5341 cataaatgga taccaataaa gataaatgtt aagataccac tcactctttg gaagaagaac
     5401 atccagtttc taaaaatcga gtaatgtcca acattttctt ttgctgtaaa tgcaatgtgt
     5461 ataccaaaca aaccgtgata taacaacgga atgtatataa ataaaaattc tacaataatt
     5521 agaaatggta atgattccat aaagttagat gccttattaa acgcttcagc accttgtgtt
     5581 gcttggtgat tcactaataa atgaacgacc aaaaatgcac ctattgggat aatacctaat
     5641 aacgagtgaa tacgtcttag ataaaattca ttttttgatt gagccaaaag gagtcccccc
     5701 tgtgaacgaa tatttaattt attgagctat ttatattaaa cgtacgctta accccctaaa
     5761 gtgatatcgt ttctagcgtt gtcattatca ttaagcgaaa cattttaaag acaaatacac
     5821 actgcacgat caccaaactg catgtcgaac aatgtaacat ttggattcga tatctaaaat
     5881 tgcttgtgat gataaacttt ctcatttaga aaacgcttcc acgtacattc aaaaaaataa
     5941 ctttgttaac catattgtaa cattatttca tatattttgg ggcatgagaa tgattctcac
     6001 gcccagtaat ttatttatgc aattgttcat gtaggttctt tgcgacgttt tcaggaatac
     6061 ctatattttt aaaatcttca agtgtagctt ccttcatttt cttgattgaa ccgaatgaac
     6121 gcaataataa tgttttacgt ttgttaccga taccatctat atcatcaagt attgatttca
     6181 agcctgtctt ttgacgtgtt tgtctatgaa atgtgattgc gaatctgtga acctcatctt
     6241 ggatacggtg caacaaataa aatgcctggc tatttttctt cagtggtaca atttctgcac
     6301 tagcgccata taataattca gatgtttggt gtttatcatt tttctgcaaa cctgcaacag
     6361 ggatatcaag acctaattcg ttttgtagca catcaataac cccgttcata tgtcctttac
     6421 caccatctac tattattaaa tcaggtaatg gtaatccttc gtttaaaacg cgagaatatc
     6481 gtcgtcttac tacttctctc attgatttgt aatcatctgg acctttaacc gttttgattt
     6541 tatactttct ataatttttc ttatctggtt taccgtcgat aaatgtaacc attgctgaca
     6601 ctggatccac accttgaata ttagaattat cgaatgcttc aattctaatt ggtgtttgaa
     6661 ttcccatttg tgttccaagt tcttcaatag ctttaatcgt tcttgactca tcacgtgata
     6721 ttaattcaaa tttattattt aaggatactt tagcgttatg tgcagctagg tcaaccatat
     6781 cttttttggg acctcgcgcg ggttgaacga ttttagtgtc cacaacagat tgaatcattt
     6841 ctttatccaa attacgtggt acatgaactt ccttaggtaa aatatgttgg tttaagctat
     6901 aaaattgtcc aataaatgta taaaattctt cttcttctgt ttgctgtaat ggaatcatcg
     6961 ttgtatctcg ctttatcata ttaccttgtc gtataaagaa aacttggata cacatccatc
     7021 ctttatcaac actataacca aagacatcac gaatcgtttt atctgatgac ataatttttt
     7081 gtttgtttgt cagattttga atatgttgaa ttaaatctct atattcttta gcccgttcaa
     7141 aatcaagtga ttcacttgca gttaacattc gctcttctaa actttttaaa attgttttgt
     7201 cttccccatt cagaaaatca gtaatttcct tcgtcatttg tgcgtattta ctcaaatcaa
     7261 cgtcatatac acatggtcct aaacattgtc caatatggta ataaagacat aatttatctg
     7321 gcatcttatc acatttgcga tatggatata ttctgtctaa taacttttta gtttcttgag
     7381 cagaatatgc attcggatac ggtccgaaat atttgccagt accttgtttt acagttctcg
     7441 tcactagtag tctaggatat ttctccttcg taattttaat aaatggataa cttttatcat
     7501 cctttaataa tatattatat cttggttgat attgtttaat cagattcaat tcaagtaaaa
     7561 gtgactctgt ttcactagac gtgacaataa actcaaagcg acgtatttca ccaaccagtc
     7621 tcgttgtttt agcatcatga gcacccgtaa aatatgatct caatcgattt cttagctttt
     7681 tagctttgcc aacatatatc acttgatcat tacgatcttt cattaaatag cagcctggtt
     7741 ccataggtac gacatttaat ttatttttaa ttcgttgctt atagtcttcc aaaaatatcc
     7801 ccccttctaa ttttaaaatg aatgatgcta caaaagtgct tcaaatttat caccactaat
     7861 tctagttaat aatcaacatc ttcgagtatg acttttcatg cacatcatgt aatattgaac
     7921 attgttttat caaaccaact gctacaaatc tatctgcatg ctagattatc atttaacgtt
     7981 catagattta ttaatcccac cattctcaag tcgtaaacaa tatgatgact tatcattcaa
     8041 ctttttatat taaaaacaca aacaaaaact ggagcaattc attaaagaaa tgccccagtc
     8101 gtcattggtt gtaacttata aatgtttatc taaaacttca gctaagtttt cttttggttg
     8161 gaaaccaaca actttatcaa ctggttgacc gtctttaaag acgattaatg ttggaatact
     8221 catcacttca tatttagctg cagttgatgg attttcatca acatctaatt ttaaaatgtc
     8281 agctttacct tcatagtcag ctgctaattc ttctaatacc ggagcgatca ttttacatgg
     8341 accacaccat gttgcccaaa aatctactag ttgtacacca gattctactt ttgaatcaaa
     8401 atctgcatct gttactttta cgattgccat aaatgccaat cctccttaat tattattatg
     8461 atgttcaaat tatatcagat aacatgcttc atgtatttct atttgctcaa tttttatgct
     8521 tatttcaaat aacatgtttg aaatgtattc ttgttgcttt acattatttt aactgcagct
     8581 atttaattta tcaaattata atttatttta gtgttgcaac ggtaacgcca aatccacctt
     8641 cgcttggcat accacctcta aagtcactaa cacttttatg ctttttcaaa tgttgttgta
     8701 cacctttttg aagtgcacct gtacctttac catgaatgat ataaacttgt tcgtaattac
     8761 ttaaaacggc ttgatctaaa tattgatcta gttcaattaa agcatcctca taacgatagc
     8821 ctcgtaagtc aagttcagtt ttaattgttt gacgattttg acgtgtaacc attttcgttg
     8881 gcttaacttt ttctttttgt tttttctcta aatcttcaat aggtaacttc attttaataa
     8941 ttcccatttg aacaattgct tcttcatcat tgacaatttc taaaacttca cccttttgac
     9001 cgtaagataa tacttttact tcatcaccag caacaatttt atcgtatttt tgcttttgta
     9061 cattttgctt tatagatttc gcttcataat gatcatctaa tcgtttcttc ttatcaatca
     9121 attcatgttc tttaacatct gcaccttttt gttcacgcaa ttgtcttaag tctttaataa
     9181 tatcgtcagc ttcttttgtt gcagccttaa tcttctgatt tgctttttct ttcgcttcct
     9241 ctattagaga cttttcataa ttttggaatt gttgatactg cttagataaa tcatcatgca
     9301 cttgctccgc ttcttttaca agacggtcca gttctaacct ctgtgtctct acacgtttgt
     9361 aattacgctc taatgattca atcatttcat ttatttcttt ttcatcagta ccaatcatcg
     9421 tcttagcctt attaataata ttcaaactaa gacctaactt tttagaaatg tcaaaagcat
     9481 ttgaacgacc cggcacaccc attaataact tatacgttgg actcaaagta tctacatcaa
     9541 attctacact cgcattcata acgccttctc gattataact atatgcttta agttcaggat
     9601 agtgcgtcgt tgccattact agagaaccaa tttttctaac atgatctaaa atgctcattg
     9661 ctaatgcagc accttcactc ggatctgtac ctgcacctaa ttcatcaaat aaaactaaac
     9721 tatgtttgtc tgcatgcttt aaaatttcaa ctatattcgt catatgagat gaaaaagttg
     9781 ataatgattg ttctattgat tgttcatctc cgatatcgca atatacattt ttaaatacac
     9841 ttaactgact accatcaagt gtgggaatca acaatcctga ttgagccata acaataatta
     9901 aacctaatgt ttttaatgtt acagttttac cacctgtatt cggtcctgta ataattaccg
     9961 tttcaatatc ttccataaat tcgatggtat tagctacaac agtctcacga tttaataatg
    10021 gatggtatgc tttaggtaaa tatacagtac ggtcctcttt aaatatcggc tttgttcctt
    10081 taacacttct actatatctc gcttttgcga ttaaaaaatc taactgaccc atgacttgtt
    10141 ctgccacaag tagtgcatct ttgtccgcag ccacataacc agttagttgc gttaaaatgc
    10201 gttctttttc aattgcttcg tcatgacgta atcgactaat ttgattattc atttcaacaa
    10261 ctgatgatgg ctcaatatac aatgtttgtc ctgaagcaga ttgatcatgt acaatcccat
    10321 taaaatcttg tcgatattca gctttgacag gtataacgtt tctttcattc ctaactgtta
    10381 caatagcatc tgataatttt ttctgatttg cttggctttt aacaatacgg tccaaatttt
    10441 gtctaatacg ttgattcgtg ctagaaattt tacttctaat cccttgcaat tcataactcg
    10501 cattatcata taaatcatac gtatcgcatg tttcatttat ttgatgaaaa agatcagtta
    10561 acacaggtaa ttgattcatc ttgtcatcta atattgggta tttaacacct tcatcttctt
    10621 caaccaattg attataaaat gtcttgaatt gattttgtac ttgaattaat ctttttatca
    10681 agttaagctc tgatacattt aaaacgccgc caatatcagc gcgatgaatg aatgctgata
    10741 ctttagataa gccactcaag cttggtaaac gatgcttatt ataaatttga gcaatctcat
    10801 ccgtttcttc catttgaaaa acaaccgttt caaaattagt agctggcatc atttgattga
    10861 ccttttccaa gcctaagtca ctaatagttt cattggcaac gagtgatttt attttttcaa
    10921 attctaagac gtctaatgtt ttttgtctca taaaatccct ctatttctta agttttgtat
    10981 tattttcaat aaagtcttta aaagcttcac gcgataatgc gttaatcact ctatcttttg
    11041 ttacaaatcc tttttgcgca gttgctacgc cataattcat aaaatctaaa tgatttgtat
    11101 gatgcgcatc agtgttaata gttaatttca catttggata tttacgaacg atatcagcgc
    11161 tcagatccag tcgatgtgga ttggcattaa tttctaatac tgtattcgtt tcttcagcta
    11221 atgccattaa ttgttcaata ttcggtttat aaccatctct tctacctata atacgccctg
    11281 ttggatgcgc tatatgtcgc acgtatggat tgcgacatgc attagctaat cgttccataa
    11341 tttgttcttc tgattggtta aagctttgat gaatagctcc aattacataa tcaagttgtg
    11401 ctaaaatttc atcatcataa tccagcgagc catcaagtaa tatatccatt tctgtacctg
    11461 aataaatatc aatttcacta tattctttat ctaaagcctt aatttcttcg ttttgtctta
    11521 aaagtctttc tacttgtaag ccattagcaa cacgtaaact ttgtgaatga tcagtaatta
    11581 ccatgaattt ataacctttt gcgatatttg cttctaccat gtctcgaata gaaaacgcac
    11641 catcactata cgttgtatgc atatgaatat caccattaat atcatctatt gtaatgatat
    11701 tacttagatc tttatcaaat tcgctaccat cttctcgcat agcaggtggt ataaaattca
    11761 cattaaaatg ttcatatatc ttggcttcac tatcatattg aattaatgta ccatcagctt
    11821 gttcaattcc atattcactt actttttcat cacgtgcttt agcaagttgt cgaattctta
    11881 tattatgttc ttttgaccca gtaaaatgct gcaatgtatg ataaaaagca cttggttcaa
    11941 ttaatcgaaa atcgacacca atcgtttcat catcatacgc taattctaat gaaacttttg
    12001 tgttccccac tgcaacttct tttactttat tgggaatatt taataattgc tgctgcactg
    12061 cttttgggtt atcggtactt attatgaaat ctaaatcttt gctcatttct ttaaaacgac
    12121 ggaagcttcc tgcagatgaa tattgatcga tataatttaa tgtatctata taatcaatga
    12181 tttcttgatt aagtcttctc atttgatcaa ttggatatct atctttctta gcaccaagtt
    12241 gtttcacagc ttctaatatg ttttgttccg ttttcttagc aaatccgctt aattcactaa
    12301 cttttccatt ttcacaagca acttgaagtg acgctttatc aacaatattc aactctttat
    12361 atagcttagc aattttcttg cttccaagtc cttgaatttt caaaagtgga ataagacctt
    12421 ccggaacttc ttcctgtaat tgctgtaaat actgagattc accggtctca cggtaatcat
    12481 tgattacttc tgcaacacct ttaccaatgc cttttaactc cgttacatca gatatttcat
    12541 ctaatggtcg ttcatctaat tcaagacttt gagctgcttt tcgatacgct gatattttaa
    12601 aagtattttc cccttttaat tccatataag tagcaatttg ttctaatagt ttgataacat
    12661 ctttttttgt cataataaca ctccataaaa agaagaccag gacgtatcat taatatatac
    12721 ctttgtcctg acctcttatg ataatttata aattaatggt gaaagccgat aaatatggga
    12781 tgtgaaaaat caatgaatga ctcacaatag atattttaag ctgatgttgt aatgcttcgt
    12841 taggatataa tgccaataga tataacgtaa agtgtaagac aattatcgtc ataaacacac
    12901 caactatcat tcccattgca cgactgaaaa tatgaatgtt ttgatacgct attattttat
    12961 caaaagttac gataattaga tataaaatga acttacaaaa caatgtaatc attaaaaaag
    13021 ctacaatcgc ttcaaatcga ttttgtagat gattaaaatg aaacgcaaaa gttgtattaa
    13081 atgctgttgt tttaggatat ggaataaata caattaatct ttctacaata gatttgtaaa
    13141 attgactggc aatccacaat gatacaatcg ttgcactcaa atgtatcata gataaccaaa
    13201 aacctcgtct gaatccaacc atgacaaaat acacaaagaa aatgattatg ataaaatcaa
    13261 tgaccattta ttgctcacgc tgctgcaatt tgtgaatttg ttgtttcaaa cgtcgatttt
    13321 cttcttctag tagtactttt tcatgcataa tattcacagc agttagtatt gcttttcttg
    13381 aagtatctaa acctgctgct ttatacccta attcttttat tttatcatca actaaatgtg
    13441 ctacatatcg tatgtgctct gggttatctt ccccaacaat tgtaaaaagc tgatcattaa
    13501 ttgatacatt taccttgttt ttaaactgtg ccatttataa tttctcctga tccttttttt
    13561 aaaatctaaa ttcacgttat aaaatatgac tggatagttt gtctgaattt gatactaata
    13621 ttgttatatt gcaattatga taaaacaaca acacaatctc tatggatgac ttaatgttct
    13681 ttttataatg aaataatgta aagaattttc tattcaatac tttatcatgt ttaaattgtg
    13741 tcactataac attttcataa acattataca tgaccactat gtattttgta agtatccgca
    13801 attaattctt tacaacatac ataaatgttt ctgacgttat tatcatttat gatatgatta
    13861 tttttgctaa agataatgaa attttatgaa aggatttaca caaatggcga atatcgtttt
    13921 taaattgtcg gataaagaca taacgacatt aatgtcacgc attacttttg atactgagaa
    13981 tttacctcaa ggtatgaaag cacgtgcaaa gtatcaaaat acaactgtaa atatttacca
    14041 atcaggtaaa gtcatgtttc aaggtaatca tgcagaagct gtgtctaaag aacttctacc
    14101 gcagcattct caattgaata ctaataaaac taaaaagaaa aatatggcta attcttttct
    14161 tgaacaaact ttaatgtatg atcaatttaa ctgtattggt agtgatgaag ctggaagtgg
    14221 cgattacttt ggtccattga ctgtatgtgc tgctttcgtt accaaagagc atgtccctat
    14281 tttaaaaact ttgggtgtag atgattcaaa aaaattaacc gataccaaaa ttgtagaact
    14341 tgctgagcaa ttggttactt tcatcccaca ttcattatta acactacaca atgaaaaata
    14401 taatattcag caagcaaaag gctggactca agtaaaaatg aaagctgttc ttcataatga
    14461 agccatcaaa aatgtacttg aaaaaattga ttcgtctcaa ttagactata tcgtcattga
    14521 tcagttcgca aaacgcgaag tgtataacca ttacgcatta tccgatatac cacttccaaa
    14581 gaaaacaaaa tttgaaacaa aaggagaatc gaagtcatta gccattgcag tggcaagcat
    14641 aatctctcga tacgcatttg ttacatatat ggatcaaata tcaaaaaata ttaatatgac
    14701 gattcctaaa ggtgccggtg ctaaagtcga tgtaatcgcc gccaaaatta taaaaaaata
    14761 cggtctttca catttagata ccatttcaaa aaagcatttt aaaaatcgtg aaaaagccca
    14821 aaaaatttta aagcctcttt gatttttaat cattataaat tggatcctaa taggaaatcc
    14881 tattaggtaa gtcatactaa tttgctttgc tcataagtat gactttatat gcagttgtgc
    14941 gaagcatcat ctgtattcca ctgttcctct ttgacttttt aaaccaatcg aatcctaatt
    15001 ggtgaatcca aaaataaaac cccatgttag ggatatcaat ctacttcaga tatccttata
    15061 catggggttt aaatcattat ctaataacag caccttgttc aattaatgct gcttcaattt
    15121 ccgcttgtac ttttgaaacg cgctcatctg tcaatgtttc ttctgtgtct aaataattta
    15181 aacgtattgc aattgatttt ttaccttttt ctaaatgttc gccctgatat acatcaaaga
    15241 caagtgtatc ttttaatata ttgccaccgt gtgcatgaat cgttgataat aaatcagctg
    15301 ctggaatatt ttgatctact tctaatgcaa tgtcacgaga catgcctggg aatctcggaa
    15361 ttggctggta attaatgtaa cctaccgaca cagacattaa tgcatcaaaa ttcaactcaa
    15421 aaacatacgt acgttttaaa tcattatcag ctgctaatgt tggatgtaat tcaccaataa
    15481 aaccaacaac tttattttct aataagattt cagctgtacg acctggatgt aatccatcaa
    15541 tatcggcacg acgataacta aattcaaggt ttaacttttc agatactcga tccacgacac
    15601 cttttgctaa atagaaatca accgtttctt tcttaccttg ccattgattg actacataat
    15661 ctccagttaa aataccactt aaatattcaa cttgatctgg tagttcacct tctccattag
    15721 caaagaagac attgccgatt tcaaataatt ttacatcttt atttttgcgt gccacattat
    15781 atgatgctgc ttcgattaaa tgtggtaata aactttgacg taatgacgca tgcgcttcac
    15841 tcattggcat caataaatca attgtttgac gctgttgcat cgaaaatgca gtagcatcct
    15901 ctttagaaac taacgaatac gtaatagctt gatctaatcc agcaccttct aacacttctt
    15961 taaccattct cgttttatat tggcgatcag ttagctgacc actagtaact ttatcgaaga
    16021 caggtaacgt tgatggaata tcgtcgtatc catatatacg tgcaacttct tcaattaaat
    16081 cttctttaat tgtaatatct ttacgacgtg atggtactag cactgtaata acatcatcat
    16141 ttatttctgt atcaaaccct agttgattaa aaatagtaac aatatcattt tgtgacaaat
    16201 caaatccaat agtgcgatta attttatcag cagtgatgtc gattggtgta ataaatgcac
    16261 caagttctcc tgaagacact ctatctttta gcacttttcc gtttgcataa gtttgtaata
    16321 aataacatgc acggtcgact gcttcatcta caaattcagt agctattcct ttttcaaaac
    16381 gactagatga ttcactgcgt aaatttaaac gtcttgatgt atgacgaatt gaaactggat
    16441 caaaaatagc accttcaatc actatatttg atgtttgttc tttaacttct gaaaaatcgc
    16501 cacccataac accagctaat gcaattggag tttgtccatt agtaatgaca atatcgctcg
    16561 ttaataattc acgttctgta tcatctaatg ttgtcatttt ttcgccttca ttagcttgac
    16621 gaacaacaat ttgttgtgaa ccaatcgcat cttgatcaaa catgtgcaat ggttgaccgt
    16681 attctaataa cacataattt gaaatgtcaa caacattatt aataggacgt atacccgctt
    16741 ttattaagcg tgcttgcatc caaattggcg agggttcaat tgtcacgtcg tgaacaacac
    16801 gtgcactata atatggtact ttatcttcat tttctattgt cacagtcagt tcatcatttg
    16861 cagataactc aagctcattt gatgttgtgt ctggcttagt catttttgta ttatacaatg
    16921 ctgcaacttc ataagcagta cctatcatac ttaaagcatc tgcacgattc ggcgttaaat
    16981 caaattccat tacttgatca tctaaatata aagcttgtaa ggcatctgtt cctggaactt
    17041 gggattcact aaatacataa atgcctgatt caaaactttt cggtatataa ttacttgaaa
    17101 taccaatttc ttgtaacgaa caaatcatac cttctgaacg ttcaccgcgt aatttggcac
    17161 gcttaatttt aataccacca ggcaatcttc cacctacttt agcaacaatg acatattgtc
    17221 ctgcatcaac gttcggtgca ccacatacta tttgtacagg ttcgtcttct ccgatatcaa
    17281 cttggcaaac atttaattta tctgcatcag gatgtttatc ttttgactta acgaagccga
    17341 caactaaatt tttgatatct tttgtgtagt caattaaatc atccacttca atacctgtgc
    17401 gcgtaatacg ttctgccaaa ttacttacag aatcatcgat tgttacatat tctttcaacc
    17461 attcatttga tatcaacatt acatgtcacc tctatcttct accgctttaa attgatctaa
    17521 aaatctcaca tcattagtat agaaatgacg aatatcttct ataccatatt tcaacattgc
    17581 aatacggtct ggtcccatac caaatgcaaa tccagagtac tctgaagaat caaaaccagc
    17641 catttctaat acattaggat gtaccattcc agcacctaaa atttcaatcc atcctgtgtg
    17701 tttacacaca ttacaacctt ttcctttaca tttaaaacat gacacatcaa cttctacaga
    17761 aggttcagtg aatgggaagt aacttggacg taaacgaatt tcacgatcag caccaaataa
    17821 tttcttagct aacaattcta aagtaccttt caaatcactc attttaacgt ttttatcaac
    17881 aactaatcct tcgatttgtg taaattgatg actatgtgtc gcatcatcag agtcacgacg
    17941 atacacttta ccagggcaaa taattttaac tggaccttga ccatgacgtg attccatcgt
    18001 acgtgcctgc actggtgatg tatgcgtacg taataaaatt tcatccgtaa tatagaaact
    18061 atcttgcata tcacgtgcag ggtgtgattt aggtaaattc agcatttcga agttataatg
    18121 atcttgttca acttcatatc cattcacaat ttcataacct aaacctaaga ataagtcttc
    18181 aatttcttct attgtacgtg ttaatggatg ctttgaaccg atttcaatat gacgacctgg
    18241 taatgataca tcaattgttt cttcagccaa ttgcttattt aatttttctt taactaacat
    18301 ctgttgtctt tcatctaatt cattttgaat tgtttgacgc aattcattca ctttttgacc
    18361 aaatgcaggt ttctcttcat tcggcaaatc cttcatcaat ttcattagtc cgctaactga
    18421 ccctttttta cctaagtatt tcactttaac ttcttgcagt gcacgttcat catttgcttc
    18481 attaatatct acaagcgctt gttgttttaa ctctgacatt gtttgttgtt cagacatatg
    18541 tcactgacct ccctattcga actattcatt ttatatttat catatttgtt gtttaactaa
    18601 aaaaagactt tcatcctctt aaaaaataag ggacgaaagt catttccgtg gtaccaccca
    18661 aatttatgcc taagcataca cttgtttaat attgataacg gattgtcttt ccgacttaaa
    18721 tctctttaag taaccaaaaa ggtgaaaaga ctttaaataa ttggaataac ttgcagtcac
    18781 gattattctc cctaaaacaa ttatttatat aaatgtccat gcctttttat tggtttatat
    18841 tcaattgtgt atgcaatcac gtagaaatgt tctgttaaac aattaattat aattatatcg
    18901 gaaaactttc aacacggtca acctttcaaa tgataaagta aaatactacc tgcaatcgct
    18961 acatttaaac tttcagcttt accataaata ggtatgatta aattttgtgt agtatgtgct
    19021 aataattcag gattaacacc ttctccctca ttacctaata gtaatgcaaa agaatcactt
    19081 gaagtaactt ctttgtatgc cactgcgttt tcaagtgctg taccataaac aggaccatta
    19141 aattgagtaa taaacgtatc gagatcttgt gtcataactg gcaaatggaa aacactacct
    19201 tgactcgctc gcaacacttt atcttgataa ggatctgtcg tacccttctc cattattaca
    19261 gcatccattc cagcagcatc cgcagttcta attaatgtgc ctaaatttcc aggatcttga
    19321 acacgatcga tgagcaatac ttgttgtgct ttagaaatat catagtgcgg cttctcaatg
    19381 attgcgaaaa acccttgtgg tgtcactgta cctgataaag attcagcaac tttcatgttt
    19441 atttcaaaaa cttcttgcgc gtatgcgata atttgctgat ctaatcttgc cggttcaatt
    19501 gcaaataatt gtgtaattac aattccactt tgataagctt cttcaattaa atgcacacct
    19561 tcaattaaag ctaatccagt tttatccctc tcacgtttct tttttagctt gttcgcttgt
    19621 ttaattctat tattttgtgc agaagtaatt tgttccata
//
LOCUS       DBNENW010000041        18433 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNENW010000041 DBNENW010000000
VERSION     DBNENW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.041"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            20..418
                     /locus_tag="AC1LW8_001124"
     CDS             20..418
                     /locus_tag="AC1LW8_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HIP4772972.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(668..1492)
                     /locus_tag="AC1LW8_001125"
     CDS             complement(668..1492)
                     /locus_tag="AC1LW8_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIP4772973.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(1731..3038)
                     /gene="brnQ2"
                     /locus_tag="AC1LW8_001126"
     CDS             complement(1731..3038)
                     /gene="brnQ2"
                     /locus_tag="AC1LW8_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein BrnQ2"
                     /protein_id="HIP4772974.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVSLLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            3622..4512
                     /locus_tag="AC1LW8_001127"
     CDS             3622..4512
                     /locus_tag="AC1LW8_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HIP4772975.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKVNLGNQNIMAVAWYQNSAEAKALYLQGYNSAKVQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKDVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            4762..5811
                     /locus_tag="AC1LW8_001128"
     CDS             4762..5811
                     /locus_tag="AC1LW8_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4772976.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            5824..6501
                     /locus_tag="AC1LW8_001129"
     CDS             5824..6501
                     /locus_tag="AC1LW8_001129"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4772977.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(6660..6800)
                     /locus_tag="AC1LW8_001130"
     CDS             complement(6660..6800)
                     /locus_tag="AC1LW8_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772978.1"
                     /translation="MARIPLGALYVNNVKITKTKNKNKVPINKSITIDPHYDNFIFNP
                     HI"
     gene            6751..7740
                     /locus_tag="AC1LW8_001131"
     CDS             6751..7740
                     /locus_tag="AC1LW8_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP4772979.1"
                     /translation="MIFTLFTYKAPNGMRAMGALANAAIATFLVEAFNKYVGGEVFGI
                     KFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMDLLPGFFAGYMIGYV
                     MKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIGDIIQSSTNTNPIIM
                     GIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAFMNGTLFHRLKLGDR
                     KSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGLINDATGTATPIAGF
                     LVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQDMINRGAVDA"
     gene            8083..9201
                     /locus_tag="AC1LW8_001132"
     CDS             8083..9201
                     /locus_tag="AC1LW8_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HIP4772980.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9176..10099
                     /locus_tag="AC1LW8_001133"
     CDS             9176..10099
                     /locus_tag="AC1LW8_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIP4772981.1"
                     /translation="MANLQKYIEYSREVQQALENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10110..11330
                     /locus_tag="AC1LW8_001134"
                     /pseudo
     CDS             10110..11330
                     /locus_tag="AC1LW8_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(11436..12968)
                     /locus_tag="AC1LW8_001135"
     CDS             complement(11436..12968)
                     /locus_tag="AC1LW8_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HIP4772982.1"
                     /translation="MKEIGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13008..13889)
                     /locus_tag="AC1LW8_001136"
     CDS             complement(13008..13889)
                     /locus_tag="AC1LW8_001136"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HIP4772983.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14047..14907
                     /locus_tag="AC1LW8_001137"
     CDS             14047..14907
                     /locus_tag="AC1LW8_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP4772984.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15184..15984)
                     /locus_tag="AC1LW8_001138"
     CDS             complement(15184..15984)
                     /locus_tag="AC1LW8_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP4772985.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLTHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16125..16793
                     /locus_tag="AC1LW8_001139"
     CDS             16125..16793
                     /locus_tag="AC1LW8_001139"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIP4772986.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYNHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(16924..18237)
                     /locus_tag="AC1LW8_001140"
     CDS             complement(16924..18237)
                     /locus_tag="AC1LW8_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HIP4772987.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6450 a         2833 c         3418 g         5732 t
ORIGIN      
        1 ataataaagg agtaagtcaa tggaaaaatc gatcaaaata atgacaataa taggaattgt
       61 tattcagggt ttagcaacgg tatttagttt actattgatg gttttagcag catcaggtgt
      121 aatgactaca gatgtgtcaa caacagttaa tggtgaggtt gacccagttg atgcagaaac
      181 agcagcagca attttcactg tattattctt attcctattc atatttggaa tcatttcaat
      241 tattttaggt gcaatcggta tgtttaaagc atctaaaaac aaaaaaatga gtggtatatt
      301 gttgattatt ggagctgtaa taagtggtaa cataattaca tttgctttat ggttagtcag
      361 tggtattaaa ctacttacta ataacaagcc taaagatgaa ataagcgact tatcataaac
      421 atcgtatatt gaaatttcaa gatttcttaa gtaattatat ttctgttagt ttttaataaa
      481 ttaaccgatt aaataaagtg ctctcttaga agtagatttt cagtcaataa ctgcttttaa
      541 gagagcattt tgtatttgat aatgctcata gcatcggaca aaaaccattg acgaacttta
      601 aaaaaatacg ttagacaatg cacttctaaa tattaaaggc atcgactaac gcattgttca
      661 aatagagtta attattttta taaaattgat gatgatcatt caagtaagca tagaataaac
      721 ctataatgag tcctcctcca atatagttac cgataaaagc cgcagcgata ttcgaaatag
      781 ctggtatgaa gtgcaatgta tcaacttgat aaattaaacc acccataaat aagcaactgt
      841 tgtaaacgac gtgttcataa cccataaagg cgaatatggt aacaccgaac atcatgacaa
      901 acatttttgc gagtacatcg tcaatttgca tggcaataac taatgaaata ttgataaaga
      961 aattggcgaa tatcgctttc attaatatac ttacaaaacc agtagacaac gttttgtgct
     1021 caataactgc tgataactga tttaacatat ctggcgtcat tacatttgaa aaacgcatga
     1081 aactaaataa aatggcagca cctaaaatat ttcctgcaaa gcacaataaa aatattttca
     1141 atactctagt cggtttaatt actttataat acaggcctac agtaaagtac atgaagttac
     1201 tggttagtag ttcggagttt gtaaataaaa tgagtactaa cgcaaagctg aatgtaatgg
     1261 cactggccat attcacaatg cctggcggta aatctggttc gtgtgttgct ttaactgata
     1321 atacgaagac cgtaataatc ccgataataa atcctgccat catcgcgcgt aataaataac
     1381 gttttaaata aacgctttgt aatatatctt ttgttcttat tgtttcgact acgttattta
     1441 cccagtcgtc cccataaaat attttatccc atttaatatg tttctccttc acaatgacat
     1501 cctcactttt catttgttaa tcagagtata ctatgaatcg tcttatttgt gaataatttc
     1561 acaaaattaa ttttaaaaac taatattttt gaataacagc taggattttt taacaaatca
     1621 tttattttaa aataattaat cgtcagttag tatcaatgaa taaatacaga ttttcataca
     1681 aaaaaaccta gtaatgatgc gacgatcatt actaggagat tatgcgtcta ttaagttgta
     1741 gcttgtttcg gacggcgtat aaatacatcg attatgaaac cgataatagc aaagagcatg
     1801 aatggtacaa gccaagctaa atcgatatct gctaaaggta acatcataaa cgatttcaaa
     1861 ataacaccgt gtaataagtt aaaactattt agtatttgta aaattgaaat aatcaatgta
     1921 ataacagttg cgagtcgata ggcccaactg aatctgaatg tgctaaacat gttagcaaat
     1981 gatatcagta caagtgcaat agacacggga tatattaatg tcaataatgg gacagcaatt
     2041 tttaaaatca tttctaaacc aagtgttgta aataagaacc ctatgataga gaaaataagt
     2101 gcgaatattt tataagaaaa cttaggtacg tgtttcttag taaatgtggc gcaagcattg
     2161 acgagtccta tacatgttgt taggcatgca aggataaccg tcattccaaa tacgaggtta
     2221 ccgaacgaac caaatactcg taatgagttg tacgtcaata tatctgtacc atctttaaag
     2281 tttcctggag ctgttgatgc cccaacgtat gcaagtgcaa agtaaatcat tccaagtaat
     2341 atggctgcaa taagacctga aaagcagaca tattttaaaa ttttcatgcg atctgtgagg
     2401 cctttaaact tatagccatt gacaatgaca acggaaaaag ctaacgcagc aacaagatcc
     2461 attgtaaaat agccttccaa acttcctgaa atgaaaggat gtgttatata tttatcctta
     2521 ggtgcactta gtgcagattc agggttgaaa atgacagcaa tacttaatag agagaccatt
     2581 aatagtaata acggtgttaa taatttacct aaattatcaa cgattttcga tggatttaaa
     2641 ctaatccagt aaacgatggc aaaaaagatt gctgcgaata taattaaagt ccattggttg
     2701 tgcacaggta aaatgtgtct tgtaccaatt tcgtacgcga catttgcagc acgtggaata
     2761 ccgtaaaatg ctccgataga catgtaaatc acgacagcaa aaataaaccc gaaccatgga
     2821 tgtatacgat tgcctacact ttcaacacct tcatcataaa atgcaacaac aataacagta
     2881 ataaagggga gtaatatgcc tgtaagagca aagcctagca taccaatcca catattttga
     2941 cccgctgtat ggccaagcat gggcgggaat attaaatttc cggctccaaa aaatagtgaa
     3001 aataacatga ggcccgaaat aataacttgt tttttcaatg taatttcctt cctaaaacta
     3061 gatattcata ataatttaaa aaatctgaaa aataaaaacg ttcttacttt atctttaaat
     3121 gacaatacta tgattctatt attttataaa aaagattgca ataataacgt cttgctatta
     3181 ttggataatg acaattaaag tgtcgttttt atgaattaaa ataagtgaaa ataagttgga
     3241 aatacgttgg agcgttgtga tttttatgag aaattgtatg agtgaggact gctatttata
     3301 aaaggtaaac tatggatgag tttatagttg ttccaatagg taaataagag atagcacact
     3361 gtacacataa tgatacgtgg atgagtgaat atgtctgata ggaaggccta gtcactcaaa
     3421 tgaatttaag tttattacgt gataaatcac aaatctctct catgtgatag gtctcccatt
     3481 aaatcatgct tatatgaaat gttcacatat ttgttagctt ttcaagaaat aatattaaat
     3541 atcttttcat agtatagcaa tattaaataa atgcatttat aattttaact gtattttaaa
     3601 tattaatcat gaggtgataa gatgaataaa atttcaaagt atattgcaat agcatcatta
     3661 tcggtagcgg ttacagtttc agcaccacaa acgacaaatt ctacagcgtt tgccaaaagt
     3721 tctgctgaag ttcaacaaac gcaacaagct tctataccag catcacaaaa ggtgaatctt
     3781 ggtaatcaaa atattatggc agtggcttgg tatcaaaatt cagctgaagc aaaagcatta
     3841 tatttacaag gttataacag tgcgaaagtt caactagata aagagattaa aaagaataaa
     3901 ggtaaacata agttagctat tgctttggat ttagatgaaa cagttttaga taattctcca
     3961 tatcaaggct atgcatcaat acataataaa cctttcccag aaggttggca tgaatgggta
     4021 caagctgcta aagctaaacc tgtctatggc gcaaaagaat tcttgaaata tgctgacaaa
     4081 aaagatgtcg atatctacta tatttctgat agagataaag aaaaagattt aaaggcaaca
     4141 caaaagaact taaaacaaca aggtatccct caagctaaga agagtcatat tttactaaaa
     4201 ggtaaagatg ataagagtaa agaatcacgc agacaaatgg ttcaaaagga tcataaactt
     4261 gtcatgctat ttggagataa tttattagac tttacagatc caaaagaagc tacagctgaa
     4321 tctcgtgaag cattaattga aaaacataaa gacgatttcg gtaagaaata tatcattttc
     4381 cctaacccaa tgtatggtag ttgggaagct acaatttaca acaataacta taaagcaagt
     4441 gacaaagcaa aagataaatt acgtaaaaat gctattaagc aattcgatcc taaaacaggc
     4501 gaagttaaat aatatatgaa ttggacgtct acatgtactt aaaggtatat gtaggcgttt
     4561 ttaaattgat ccttaaattt taaatgtacc gtacaaaaat aatagacaat taattgcatt
     4621 ttccataaat ttgcttttaa tataaaaagt tcgggtataa tgaaggataa gtgtatgtat
     4681 aacattacag agcgcaacac gacgtacgtg catctaagaa tataaacaat gtttaaatca
     4741 tagtagtagg gagagaaatg catgttttta gcttggaatg aaatacggcg caacaaattg
     4801 aagtttggac taattattgg tgtgttaacg atgattagtt acttgctatt tttattatct
     4861 ggattggcga atggtcttat caatatgaat aaagaaggta ttgataagtg gcaagcagat
     4921 gccattgttc taaataaaga tgccaatcaa actgtgcaac aatctgtttt taacaagaaa
     4981 gatattgaaa ataaatacaa gaagcaagct actttgaagc aaacagggga aattgtgtct
     5041 aatggccatc aaaaagacaa tgttttagtg ttcggtgttg aaaagtcatc atttttagtt
     5101 ccgagtttaa tagaagggca taaagcgact aaagataatg aagtgttagc tgatgaaaca
     5161 cttaaaaata aaggatttaa aattggcgac acattatcac tatctcaatc agatgaaaaa
     5221 ttgcatatcg taggttttac agaaagtgca aaatataatg cgtcaccagt cattttcacg
     5281 aatgacgcta ccattgccaa gatcaatcct agattgactg gagataaaat taatgcagtt
     5341 gttgtacgtg atacaaattg gaaagacaaa aaattaaacc aagagcttga agcggtaagt
     5401 attaatgact ttattgaaaa tttaccaggt tataaaccac agaacttaac attaaacttt
     5461 atgatttcat tcttatttgt catttcagct acagttatag gcattttctt atatgtcatg
     5521 acattacaaa aaacaagttt atttggcata ttaaaggctc aaggatttac gaatggctat
     5581 ttagcgaacg tagttatttc gcagacgctc atattagcat tatttggtac ggcatttggc
     5641 ttactgttaa caggcgttac aggtgcattt ttacctgatg cagtacctgt caaattcgat
     5701 gtactaacat tactcgtatt tgcaattgtg ttaatgattg tctctgtatt aggaagttta
     5761 ttctccattt taacaattag aaaaatagat ccgttaaagg cgattgggta ggaggtgtag
     5821 caaatgttga aatttgaaaa tgtaacaaag tcatttaaag atgggaatcg taacattgaa
     5881 gcggttaaag atacaaattt tgagataaat aaaggtgata ttatagcatt ggttggacct
     5941 tctggctctg gtaaaagtac atttctaact atggcaggtg ctttacaaac gccgacatct
     6001 gggcacattt taatcaataa ccaagatatt acgacaatga agcaaaaagc attggcaaaa
     6061 gttagaatgt ctgaaatagg ttttatttta caagctacaa accttgtgcc atttttaacg
     6121 gtaaagcaac aatttacatt attgaaaaag aaaaataaga atgttatgtc taatgaagac
     6181 tatcagcaac ttatgtcaca attagggcta acttcattgc ttaataagtt accttcagaa
     6241 atttcaggtg gtcagaaaca acgtgtggcg atagctaaag cgttatatac gaatccgtcg
     6301 attattttag cggatgaacc taccgcggcg ttagatactg aaaatgcgat tgaagtcatt
     6361 aaaattctac gtgatcaagc caaacaaaga aagaaagcat gtattattgt tacacatgat
     6421 gaacgactta aagcatattg tgatcgttca tatcatatga aagatggcgt ccttaatctt
     6481 gaaaatgaaa cagtagaata gttttattaa gccggtacat catgtgccgg tatttttatg
     6541 tttatgtatt atttgaataa actttcacat tcaattaata ataattatta tcgaaaatta
     6601 gaaatattcc gtgaaatata atattttttg tagtaaaatg gcctctaagt attcaatatt
     6661 taaatatggg gattgaatat aaaattatcg taatgggggt caatggttat ggatttattg
     6721 ataggtactt tatttttatt tttggtctta gtgattttta cattatttac atataaagca
     6781 cctaatggta tgcgtgccat gggagcatta gctaatgcag caatcgcaac atttttagta
     6841 gaagcattta ataaatatgt tggtggcgaa gtattcggta ttaaattttt agaagagcta
     6901 ggagacgctg cgggaggtct aggtggtgtc gctgccgctg gattaacagc attagctatc
     6961 ggtgtgtcac cagtatatgc attagttata gcagccgcgt gcggtggtat ggatttatta
     7021 ccaggtttct ttgcgggtta tatgattgga tatgtgatga aatatacaga gaaatatgtg
     7081 ccggatggtg tcgacttaat tggatcgatt gtcatcttag cgccattagc tcgtcttatt
     7141 gcagtattat taacgccagt agtgaatagt acattgattc gaataggtga tattatccaa
     7201 agtagtacga atacgaatcc aattatcatg ggtatcattt taggtggtat tatcacggtt
     7261 gtcggtacag cgccattgag ttcaatggca ttgacagcat tattaggttt aacgggtgta
     7321 cctatggcta ttggtgccat ggcagcattt agttcggcat ttatgaatgg gacgctattc
     7381 catcgcttaa aattaggtga tcgtaagtct acgattgcag taagtattga acctttatca
     7441 caagcagata ttgtatcagc caatccaatt ccaatctata ttacaaattt ctttggtggt
     7501 gcgattgctg gtttaattat tgctatgtca ggtttaatta acgatgcgac aggtacagct
     7561 acaccgattg caggattttt agttatgttt ggatttaatc atccgacgac aattgtgatt
     7621 tatggtgtag taatggcgat tgtaggtgcg cttgcaggtt atcttggttc aattgtattt
     7681 aaaaaatatc caattgttac taagcaagac atgattaatc gaggtgcagt agacgcatag
     7741 catcatcata ttgaatagta aaaacaaata aaacatagta acgtgattca gtcgatgtaa
     7801 cagtcgataa tgagtcacgt ttttttatag aaaaatataa gacataaaaa tgtcataatt
     7861 tattgtcgac aaatatcata ctgtataaac atttatcatt ttctcaagta ccttttacac
     7921 gatggaatga acttactttt tacgaaatta tgcgtatttt ataaacaaat atcattgata
     7981 taacggtaaa tgtaagcgtt tacaacagaa ataacagcat gctacgatat ttttgtgaat
     8041 tcactgattc aagtatttta agtcaatatg aggagggatg ttatgagcga ttctgagaaa
     8101 gaaattttaa aaagaattaa agataatccg tttatttcac aacgtgaact tgctgaggca
     8161 attggattat ctagacccag cgtagcaaac attatttcag gattaataca aaaggaatat
     8221 gttatgggaa aggcatatgt tttaaatgaa gattatccta ttgtttgtat tggcgcagcg
     8281 aatgtagatc gtaagtttta tgtgcataaa aatttagttg cagaaacatc aaatcctgta
     8341 acgtcaacac gctctattgg tggcgtagca agaaatattg ctgagaactt aggtaggctt
     8401 ggcgaaacgg tcgctttttt atctgctagt ggacaagata gtgaatggga aatgattaaa
     8461 cgattgtcca caccatttat gaatttggat catgttcaac aatttgaaaa tgcgagtaca
     8521 ggttcatata cagctttaat tagtaaagaa ggcgacatga catatggctt agcagatatg
     8581 gaagtgtttg actacattac gcctgaattt ttaattaagc gttcacactt attgaaaaag
     8641 gctaagtgca ttattgtcga tttgaattta ggcaaagagg cattaaactt cttatgtgcc
     8701 tataccacga aacatcaaat caaattagtt atcaccacgg tttcttctcc aaaaatgaaa
     8761 aatatgcctg attcattaca tgctattgat tggattatca cgaataaaga tgaaacagaa
     8821 acatacttaa atttaaaaat agaatctact gatgatttaa aaatagctgc taaacgctgg
     8881 aatgatttag gtgttaaaaa tgttattgtg acaaatggcg tgaaagaact catttatcga
     8941 agtggtgagg aagaaatcat taagtcagtt atgccatcaa atagtgtgaa agatgttaca
     9001 ggtgcaggcg attcattctg tgctgcagta gtgtatagct ggttaaatgg gatgtctact
     9061 gaagatatat taattgctgg tatggttaac gcaaagaaaa cgatagaaac gaaatataca
     9121 gttaggcaaa acctagatca acagcaactt tatcacgata tggaggatta taaaaatggc
     9181 aaatttacaa aagtatattg agtattctcg agaagttcag caagcactgg agaacaatca
     9241 accgattgta gcattagaat caacaattat atcgcatggt atgccgtacc cacaaaatgt
     9301 tgaaatggca acaacagtag agcaaattat caggaataat ggtgccattc cagcaaccat
     9361 agccattata gatggcaaaa ttaaaattgg tttagaaagc gaagatttag aaatactggc
     9421 aactagtaaa gacgttgcta aagtatctag aagggattta gcagaagttg ttgcgatgaa
     9481 gtgtgttggt gctactactg tagcgacgac gatgatatgt gctgcaatgg ctggtattca
     9541 attttttgtt acaggaggta ttgggggcgt ccataaaggt gcagaacata cgatggacat
     9601 ttcagcagac ttagaagaac tgtctaaaac aaatgtcact gttatctgtg caggtgccaa
     9661 atcaatttta gacttaccta agacgatgga gtatttagaa acaaaaggcg ttccagttat
     9721 tggatatcaa acgaatgaat tgccagcatt cttcactcgc gaaagcggtg ttaagttaac
     9781 aagttcggtt gaaacgccag aacgacttgc tgacattcat ttaacaaaac agcagttaaa
     9841 tcttgaaggt ggcattgttg ttgctaatcc aattccatat gagcatgcct tatcaaaagc
     9901 atatattgag gcaatcataa atgaagctgt tgttgaagcg gaaaatcaag gtattaaagg
     9961 taaggacgcc acaccgttct tgttagggaa aattgtagaa aaaacgaatg gtaaaagttt
    10021 agcagcaaat ataaaacttg ttgaaaacaa tgcggcgttg ggtgctaaaa ttgctgtcgc
    10081 tgttaataaa ttattgtagg tgatgataca tgaatatttt attcgctatc acagggatag
    10141 catttgcact atttgttgcg tttttattca gttttgatcg taaaaacata gacttcaaaa
    10201 agacgttaat aatgatattt attcaagtgt tgattgtgtt atttatgatg aacacaacga
    10261 ttggtttaac aatcttaact gcattaggct cattttttga agggttaata aatgttagta
    10321 aagcaggtat aaactttgtt tttggagata tacaaaataa aaatggcttt acgttctttt
    10381 taaatgtgtt gctgccatta gtgttcattt ctgtattaat aggcattttt aattatatta
    10441 aggtattacc atttattatc aaatatgtag gtatcgctat taataaaata acgagaatgg
    10501 ggcgcttaga aagttacttt gctatttcaa cagcaatgtt tggacaacca gaagtatatt
    10561 taacaataaa agatattatt ccgagattat ctagagcgaa attatataca attgcgacgt
    10621 ctggtatgag tgctgttagt atggcaatgt tgggttaata tatgcagatg attgagccca
    10681 agttcgtagt tacagctgta atgttaaata tttttagtgc gcttatcatc gccagtgtaa
    10741 tcaatcccta taaatctgat gatagtgatg ttgaaattga taacttaact aaatcaacgg
    10801 aaacgaaatc agtgaatgga aaaacaggaa aacctaagaa agttgccttt ttccaaatga
    10861 ttggtgatag tgcgatggat gggtttaaaa tcgctgttgt agtagccgta atgttgttag
    10921 catttatttc attaatggaa gcaattaata tcatgtttgg tagtgttggt ttgaacttta
    10981 aacagcttat tggctatgtg tttgcaccaa tcgcattctt aatggggatt ccatggagcg
    11041 aagctgttcc agctggctct ttaatggcga ctaaattaat tacaaatgag tttgtagcaa
    11101 tgcttgattt taaaaatgtc ctgggtgatg tatcagctcg aacacaaggt atcatttcag
    11161 tttacttagt aagcttcgct aattttggta cggttggtat catcgtaggt tcaattaaag
    11221 gcattagtga taaacaagga gaaaaagttg catcctttgc aatgaggttg ctacttggtt
    11281 caactctagc ttctatcatt tcaggatcaa tcattggcct agtattataa tttaatcgaa
    11341 gtacctaaat taaattcata acaacatcaa aaccccgtca ccaagttggc gcaacagcgc
    11401 atcataactt agtgacgggg ttttatcata acaatctact ttttcgtagc cgtttttgaa
    11461 atgtatgttg atggtttatc tttttcaaaa attgttaatc ccgttatatc ttttttatgt
    11521 tttgaaggga caatgaagct aagtatataa gcaaagacaa aagcaactgt aaatgaaatg
    11581 gtagatacat agaaaggtga gttacctttg ccaacaccat tatagacata agcaaagatg
    11641 atacccaata ttaatccaca aataacaccg aatgtattcg tacgtttagt gaaaatacca
    11701 actgcaaata caccagccaa tggaacgccg aataatccag tcacaaacaa gaataaatcc
    11761 cataagtcat ttgaattaga agcaattaag tatagtgaca ttccaaaacc gaaaatacct
    11821 gcaatgatga taatgaaacg tgcaaagtta acttcgtgtc gctcgctacc ttttccgaag
    11881 aagcgttgct taatgtcgat tgaaatacaa gcagatatag aatttaaact agatgaaatg
    11941 gtagactgtg cagcggcgaa aatggctgca ataagtaatc ctgctacaaa tggtggcatc
    12001 tcagtcaaaa tgaaatatgg cactacagat gatgtattga agccttttgg taaaacagct
    12061 tcatgtgtat aaaatgaata cagcattgta cccataccat aaaataaggg ggctgaaatt
    12121 aaagctagga taccatttgt ccataacgat ttatttgttt cttttaaact atcagaagct
    12181 tgataacgct gcacgacgtc ttgactcgct gtgtattgat acaagttgtt gaaaatattt
    12241 cctaggaaaa taattggaat ggcagctgcc gcagtattta gtttccaatt gtctgcacta
    12301 attaattttt tgtgctcaat cgcatctgca aagacagtgc caaaaccgcc tttaatgttc
    12361 ataacaccta gaataatgat aactaaagcg ccgcctaata aaatgacgcc ttgaatgaaa
    12421 tcactccaaa ccacaccttc gaatccacct aaaaatgtat ataaaataca tagtaaacca
    12481 acgagtgatg caacgatata agggttcatg tctgatacag atgtgattgc taatgttggt
    12541 aagtagataa cgattgcaac acgccctaaa tggtaaacga caaataataa tgagccaata
    12601 acacgtatgc tagggccaaa tctagcttct aaatattcat atgctgatgt tacttttaac
    12661 tttttaaaga aagggacata gaaataaatg agtaatggaa taattgcgac gatagcaatg
    12721 ttaccagcga tatatgacca atctgttaaa aatgctttct ctggtgtcga cataaatgta
    12781 atcgcactta acgtagtagc ataaattgaa aagccaacta cccaagatgg caagcgacca
    12841 cttgcggtaa agaaactatt ggtactttgg ctcgcgcgct tggtaaaata aacgccaatg
    12901 aacaacatag ctagtagata aatgataacg gcaacccagt ttagtgtgcc aaatccaatt
    12961 tctttcatgg gcaacatccc ctttacaatg tattgattct ttgatgtcta taaatcgtat
    13021 tttgcaatga gttgatctaa tgtttgtcga tgtgcttcgt taaaaggttt gaaaggtctt
    13081 ttcggtaatc ctgcatcaat gccacgatga cgtaatattt ctttcaatgt tggataaatc
    13141 cccattgata acactgtttc gataatgtcg tttgaatcat gttgtagttg gtaagcttct
    13201 tgaatttgac cttgtcttgc taagtcgaag atttttctag cgcggcgacc attaacgtta
    13261 tatgtagaac caattgcacc atctacgcca gaaatcgtag cttgaactaa catttcatca
    13321 aagccagata agattaattt gtctgggaat gcttttctaa tacgttcgag taggaagaag
    13381 tttggcgctg tatatttaac accaacaatt ttttcatgat taaatagctc gctgaattgt
    13441 tcaatagaaa tattcacacc tgttaaatct ggtattgcat aaataatcat attgttctga
    13501 gttgcttcga taatatcgaa atagtaatct ctaatttctt caaaagtaaa tggatagtag
    13561 aatggtgtta cggcagaaag tgcatcataa ccgagttctg tggcatattt tccaagttca
    13621 atggcttcat ttaaatctaa cgaacctact tgagcaatca atttcacttt atccccaact
    13681 gcctctttgg caactttgaa aacttgcttc ttctgctctg tatttaataa aaagttttcg
    13741 cctgagctac catttacata aagaccgtct aattcttcag tttcaatggc attttgagca
    13801 atttgtttga gtccttgttc atttacttga ccattttcat caaaaggaac gagtaacgct
    13861 gcatataaac cttttaaatc tttgttcatt atgaagtccc tccaaaaatc atttgataat
    13921 atagtttaca gctataattg taaacgctat cataaaatgt aacaatatct ttttgaaaat
    13981 tgtagtcata tttatgtata attaatgaaa atgtttttca aaatcaatag aaatggagtg
    14041 agtaaggtgt attacatcgc aatcgatatt ggaggcactc aaattaaatc ggcagttatt
    14101 gataagcaat tgaatatgtt tgactatcaa caaatatcaa cgccggacaa caaaagtgag
    14161 cttattactg acaaagtata tgagattgta acaggatata tgaagcaata tcagttgatc
    14221 caacctgtca taggtatttc atcagcaggc gttgttgatg aacaaaaagg cgaaattgta
    14281 tacgcagggc caaccattcc gaattataaa ggtactaatt ttaagcgatt attaaaatca
    14341 ctgtctcctt atgtcaaagt aaaaaatgat gtaaacgctg cattactagg cgaattgaaa
    14401 ttacatcaat atcaagcaga acggatcttt tgtatgacgc ttggtacagg cattgggggt
    14461 gcgtacaaga ataatcaagg tcatattgat aatggtgagc ttcataaggc aaatgaagtt
    14521 gggtatttat tgtatcgtcc aactgaaaat acaacgtttg agcaacgtgc tgcaacgagt
    14581 gcattgaaaa agcgcatgat tgccggagga tttacgagaa gcacacatgt gccagtattg
    14641 tttgaagcag ctgaagaagg tgatgatatt gcaaaacaaa tattgaatga gtgggcagaa
    14701 gatgtagcag aagggattgc ccaaatacag gtcatgtatg atccagggct tatattaatt
    14761 gggggcggta tatctgaaca aggagataat ctcattaaat atatcgagcc gaaagttgca
    14821 cactatttac caaaagacta tgtttatgca ccaatacaaa cgactaagag taaaaatgat
    14881 gcagcattat atggctgttt gcaatgatag ttgaaagaag gagtcattct aaaatagaat
    14941 ttgaaaccgt tacgagagat gagagctgtt gttagttcca cacatcacac tctatctagg
    15001 accaatctaa actatatcaa ccaacagtgt gccacagaca tattaaattg aagaagctga
    15061 gatattaaaa ttttagaaaa tgaaaaaaat atttggtatt gaaattaaaa aagcacctag
    15121 caactcgttg ggacaatcac gatgattgtc tacagttgca ggtggatttg aatatactac
    15181 tagttatttg ttgtctagga taatagattt agtatgttga taagtttgac tcagattcgt
    15241 attttctaat aaatgatagc tcacgatatc gattaaaaag agtgtcgcaa tttgtgtgtt
    15301 gataaattga tggtcggtat tacgcgattg atccgttgtt aaaagtacta aatctgcaca
    15361 atctgtaagt ttgctacctt cgaaatttgt gatggcaacg acatatgcac catgagattt
    15421 ggcgacttcc gctgcagaaa ttaattccga agtattacca ctatttgaca tagcaataaa
    15481 catatccgaa tgagatagta gggatgccga tattttcatt aaatgtgaat cggtagtaac
    15541 attacctttt agccccatac gaatcatacg ataataaaat tcagtcgctg ataaaccaga
    15601 gctacctagt ccagcaaaga gtatatgtcg acttgattga agtttgtcga taaaggtttg
    15661 gataatgtcg ttatcaataa attcaccagt ttgttgaatg atttgttgat gatatttatg
    15721 aattctttga ataattgggc tattttcaat aactgtctct gtcatttctt gttgaatatt
    15781 aaattttaaa tcttggaaat tctcataatc cagcttatga ctaaagcgtg tcatcgttgc
    15841 tggtgatgta ccaatcgcat gggttaagga gttaatcgtt gaaaaggcat cgctataacc
    15901 attttgtctt atataattga cgatgcgttt atcagttttt gtaaataaat gttgataacg
    15961 ttgaacacga ttctcaaatt tcattgtgtc accccttcat cttaatgatt actattatat
    16021 atgaaaaata ttttcaagat agtaaaaagc attgataaaa attatcttaa tgatatattg
    16081 taaatgactt tacgtgaaaa aaacgactta tggagtgagg aataatgtta ccacatggat
    16141 taatagtatc ttgtcaggca ctaccagatg aaccattgca ttcatctttt attatgtcga
    16201 aaatggcatt agctgcgtat gaaggtggtg ctgttggtat tcgcgcaaat actaaggaag
    16261 acattttagc aattaaagaa acggtagatt taccagttat tggcattgtg aaacgtgact
    16321 ataatcactc agatgttttc attacagcaa cgtcaaaaga agttgatgaa ctgatagaaa
    16381 gccaatgtga agtcattgca ttggatgcaa cgttacagca acgtccaaaa gaaacgttag
    16441 acgaattagt atcatatatt agaacacatg caccgaacgt tgaaatcatg gctgatatcg
    16501 cgaccgttga agaagctaaa aatgccgcac gacttggctt tgattatatt ggcacgacgt
    16561 tacatggcta tactagttat acgcaaggac aattacttta tcaaaatgac ttccaatttc
    16621 taaaagatgt actacaaagt gttgatgcaa aagttattgc ggaaggtaat gtcattacac
    16681 cggatatgta taaacgcgtg atggacttag gcgttcattg ttcagtcgtt ggtggtgcga
    16741 taacacgacc aaaagaaatt acgaaacgtt ttgttcaagt tatggaagat taaatgataa
    16801 cgataaaaaa acgagatgac catcattaat taaaggtacc taattatctt aggtggctga
    16861 atgaatgtaa tgggttcatc tcgttttgtt tgtttacgat agtgatttta ttttcaattt
    16921 gaattatcca aaaataagta aagcgacggg gatggtgatt aatagcgaca atgccacgcg
    16981 taaaaaccaa atgatgatga gtttccagac aggtatttta atttcagttg ctagtataca
    17041 tggcactaat gctgagaaaa agataatggc tgatacgctt actacaccga cgacaaattt
    17101 agtactcatt gcagctttag ttactaacaa agatggtaga aacatctcta caatagaaat
    17161 cgctgacgct tttgcaagta aagcctgatc agcaattggg aaaatataaa taaatggata
    17221 gaagatatag ccaagccaat caatgaatgg tgtatagttc gctacaatca gtcctaaaaa
    17281 accaatcgat aatatagaag gcaaaatacc aacagtcatt tctaaaccgt ctttcaaatt
    17341 gtcccaaacg ttcttcacga gggatggtgt taatgcattt tgtttcatcg cctctgcata
    17401 tgcagttttc agtctgcttc cttcaatagc aacttcttgt tctccttctt gtccgttata
    17461 atattctgtt gattcattgg tgattggcgg tagccatgca gtaattgcag tcacgacaaa
    17521 tgtgatgact aaagttatcc aaaagtataa attccaatgc ggcattaatc ctaaagtttt
    17581 agcaacgata atcataaaag ttgctgaaac tgttgaaaag ccagtcgcaa taatcgtggc
    17641 ttctcgtttg ttgtacatcc cttgcttata gacacgatta gtaatcaata atcctaagga
    17701 ataactgccg acaaacgaag ctactgcatc gacagcggat tttcctggtg ttttaaaaat
    17761 aggtctcata ataggctcca tataaacacc gacaaattct aataagccat agcctactaa
    17821 taaagaaagc gcaattgcac ctactggaat taagatactt aatggcatca ttaatttttc
    17881 aaacaagaac ggaccatagt tagctttaaa tagtattgat ggaccgattt taaatacata
    17941 cattatacca atcattgcac ctgcaacttt aaataatgta atgaccaagt ttgtgattga
    18001 agtcataaaa gtacgtctca ctattggtaa cgctgtacca attaaaatca taatcagtgc
    18061 aacatagggc ataagtggac ctatgattga gcgaatggct agatgaacat gatcgacgaa
    18121 aatagtgttg ttaccattaa tcgtaaaagg aataaagaaa catagtatgc ccactaaact
    18181 atagacaaaa aaacgccatg cacttggttg ttgtgcatta gaatgatatt gattcattaa
    18241 agcaacccct ttgtttaaat gaatacacaa aactgtatga tgcatcttcc ccttaatgag
    18301 atgaatcatt attttaattt agaaaaatct gaaaacttat tataattgta tagtttgaat
    18361 tattttcaga ccaatacaaa ttaactaatt atatatagat tgaaactata ttacttaata
    18421 aaatatttat ctt
//
LOCUS       DBNENW010000042        18233 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNENW010000042 DBNENW010000000
VERSION     DBNENW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.042"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(147..1502)
                     /gene="brnQ1"
                     /locus_tag="AC1LW8_001141"
     CDS             complement(147..1502)
                     /gene="brnQ1"
                     /locus_tag="AC1LW8_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein BrnQ1"
                     /protein_id="HIP4772988.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            complement(1778..2335)
                     /locus_tag="AC1LW8_001142"
     CDS             complement(1778..2335)
                     /locus_tag="AC1LW8_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIP4772989.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(2402..4042)
                     /locus_tag="AC1LW8_001143"
     CDS             complement(2402..4042)
                     /locus_tag="AC1LW8_001143"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HIP4772990.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEHAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLTSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENQYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            4235..4336
                     /locus_tag="AC1LW8_001144"
     CDS             4235..4336
                     /locus_tag="AC1LW8_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772991.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(4315..6360)
                     /gene="ptsG"
                     /locus_tag="AC1LW8_001145"
     CDS             complement(4315..6360)
                     /gene="ptsG"
                     /locus_tag="AC1LW8_001145"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0055056 - D-glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - D-glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HIP4772992.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            6945..8000
                     /gene="mupG"
                     /locus_tag="AC1LW8_001146"
     CDS             6945..8000
                     /gene="mupG"
                     /locus_tag="AC1LW8_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-N-acetylmuramidase"
                     /protein_id="HIP4772993.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            8000..8896
                     /gene="murQ"
                     /locus_tag="AC1LW8_001147"
     CDS             8000..8896
                     /gene="murQ"
                     /locus_tag="AC1LW8_001147"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIP4772994.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEGLITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            8908..10362
                     /locus_tag="AC1LW8_001148"
     CDS             8908..10362
                     /locus_tag="AC1LW8_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIP4772995.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            10365..11240
                     /locus_tag="AC1LW8_001149"
     CDS             10365..11240
                     /locus_tag="AC1LW8_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP4772996.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(11434..11715)
                     /locus_tag="AC1LW8_001150"
     CDS             complement(11434..11715)
                     /locus_tag="AC1LW8_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772997.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            11928..14717
                     /locus_tag="AC1LW8_001151"
     CDS             11928..14717
                     /locus_tag="AC1LW8_001151"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIP4772998.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEVYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(14864..15463)
                     /locus_tag="AC1LW8_001152"
     CDS             complement(14864..15463)
                     /locus_tag="AC1LW8_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4772999.1"
                     /translation="MSLSKLPLLNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            15589..16344
                     /locus_tag="AC1LW8_001153"
     CDS             15589..16344
                     /locus_tag="AC1LW8_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HIP4773000.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDKVNLSITSTGKTGAGI
                     IKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELSKYG
                     VIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIKRIN
                     GDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            16341..17021
                     /locus_tag="AC1LW8_001154"
     CDS             16341..17021
                     /locus_tag="AC1LW8_001154"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773001.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILDGR
                     IQHEEVIK"
     gene            17018..18196
                     /locus_tag="AC1LW8_001155"
     CDS             17018..18196
                     /locus_tag="AC1LW8_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773002.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
BASE COUNT         6391 a         2706 c         3451 g         5685 t
ORIGIN      
        1 gtgtttgtat taatgtgatt tgtaaaatga aagtactaat attttttgtg gataatcatt
       61 gatatgtatg tatacgattg cagtttaatg tagtatgaat aaaaaatgcc acaagcatat
      121 gtttaacata tgactgtggc atagtattat gcttgtggaa ttttacgatg cttaatttta
      181 taaataatga agccgataat gaaaccaatc aaactgagaa caacccagcc cataccaatg
      241 tctgataatg gtaaatattt ttggctgaaa ttaatcaaag tttgtgagaa tgatgtgctt
      301 gaaatgaact ctggactagc ttttaatcca tctactaatg cagcaatcat tgtaaagaaa
      361 atggtacatt gataaataag ttttgaatga tggaatttgc tactaaataa tgttagtaca
      421 atcaaggcaa ttgctaatgg atataagaac attaacactg ggactgagta cataataatc
      481 ttagttaaac caacattcgc gaataagaac gaaataaagc ttacaactgt tgcaatcgct
      541 aggtaattca ttttagggaa aaggtgttcg aatgtttctg aaaatgccgt aatcaaaccg
      601 atggctgttt ttaaacaagc aaccataacg ataagtgaca acaggacgat accgtagtta
      661 cctaagtagt attgagtaat ttgcgctaag gcaataccac cattttcact aagtttgaaa
      721 tgaccaatac ttaatgtacc catgattgct agtagggtat aaatgatccc catcataatg
      781 atactgatag taccagactt aattgtttct ttagcgatat cagttggatt ttcgatacct
      841 aactttttaa tcgttgcaac aatgataata ccaaatgcca atgacgctag cgcatctaag
      901 gtattgtatc catctaaaaa gccgttaaat aaggcatttg attgatattg tttactaata
      961 ggtgcatcag atatgccacc taatggatgg ataaaagcaa ataataaaat aattgctaat
     1021 aatactaaga ataccggatt taaaaattta ccgatatatt ctaaaattct tgatggcttt
     1081 ctcgcaaaaa accatgcaat cacaaagaag acgaagctaa aaataaataa atataaagtg
     1141 atttgctttg gtgataaaaa tggcgaaaat gcaatttcaa atgatgtcgt tgccagtcta
     1201 ggtaaggcga aaaatggtcc gataactaga tataaggcaa tcgtgaaaat gtaagcatat
     1261 gttttattaa cacgcgatgc aatttcaaat aaaccagatg tctttgaaat gccaatagca
     1321 atgataccta gaaatggtaa gccaattgct gtaattaaaa atcctaagtt agcgataaaa
     1381 acgttagaac cagcagcttg acccaagtgt attgggaaga taagattgcc ggcaccaaag
     1441 aataaaccaa ataacataga acctataaac atgttttctt taaatgttag tttcttcttc
     1501 atatatgtat ttgttctcct ttttagaact tataaaaatc tacttaatta agataatatc
     1561 aaaaattaaa cataaagtga tacataattt tgcaataaat actaaatttt ttgaaaattc
     1621 cggaataaat actgtttttt atgtagtagg ggcgtaagtt aagaacgatt taacagatat
     1681 agaatatgtt aaatatagaa ggttagacaa cccgacgtat taatactttg tgaaataaag
     1741 atccccctca acatgatcga aggggggaag tatatgttta gttcaatata tttagaaagt
     1801 cgtttgtagt tattgtttgt cccatcaatg ggaatacatt atctattgga aattgatgta
     1861 gcgtttcgtt ttgtgcactc atcatatctg taacaaaaaa ctgattgtag tttaattgat
     1921 aggcatctcg cgctgtcgta tctacgccaa tatgcgttgc gacaccacca agaacaatcg
     1981 tatcaattcc tcgacgtcgc aattgtaagt ccaaatctgt tcctacaaat gcactaaaat
     2041 gtcgtttgtc tatgacaaaa tcgtcatctc tcttgtctaa taaatgatgg aaacgactgt
     2101 agtcgtcgcc ttcttttggt ggtaatgaga tcattgcatt tggttgcaat acatctttac
     2161 catcatagaa attcacgcga acaaaagcga taaagccatt gttttttcta aaaacatcta
     2221 ttaatttatt agcgttttga acgacatttt cagctgtata tggggcataa tccattttaa
     2281 gaataccttc ttgcaggtcg attaacacta aagcggtttt attaaaatta atcatcttat
     2341 atcagagtcc tattcgttta ataaagtatt cgcttaaact tataccctgt atacgatgaa
     2401 attatttatt ttgttgtgaa aaagctttag cgatatcgat gagtttcttc ggtgcgtctt
     2461 cgacagacat tttgacttcg acaaaatgca tcacatcggg atgaccatta attgcattaa
     2521 acgtgtcttg taaatctttt gatgattcaa cgtcatgaat ttcaacattt ttaccaccaa
     2581 atacagctgg taaagcttta taatcccaca tgtgaatttc attataaggt tcatacatgc
     2641 cgtgaataag tcgttctacc gtatagccgt cattattaat cacaaataat accggtttaa
     2701 tatgctgtct aatcatagtt gaaatagctt gaacagttag ttgcaatgag ccatcaccaa
     2761 ttaataataa gttacgacga tctttgtctg ctaattgtga acctaatgtt gcaggtaatg
     2821 tatagccgat agaaccccat aacggttgcc ctataaaagt attgtttttg tataatgcta
     2881 aatcataagc accaaagaat gatgtacctt gatcagcaat gatgacatca tttggtttta
     2941 agaaattttg catcatttta aaataagttt gttgtgttaa tggttctgtg ccaacagtat
     3001 aatcgggtga tgttggacga tgatacgcag ggaacgttgc gttattcgta tatgaaatat
     3061 tggataactg ttttaacaat gatggtagag atatttcatc atttgtaaca tcgtcaattt
     3121 tgatattgtg atgatttaac ataacgacat catcgatatt gaattggtat gaaaaacctg
     3181 ctgttgctga atctgttaat ttggctccaa tatttaaaat taaatcgctg ttgtccacat
     3241 aatctcgtat tttatcttcg gcaattttcc catcgtaaat acccatataa tattgatttt
     3301 cctcattaaa agcacctttt cctaatgaaa gttgtgctac tggtatctgt gtttgattta
     3361 caaaatcttc taattcttga tgaaggtgaa aactgttgat ttcatgtcca gtaatgatga
     3421 taggctgctt cgattgatgc aatttagatg ttaataactc tatatatgtt gatgcatccg
     3481 tatctttggc tgccgttact tcaaatggtg tcggtatctc aatttcagag attgcgacat
     3541 cgattggtaa atgtaaatga actgggcgtc tttcggcgat tgctgtatta attaaacgtg
     3601 gtatttcggt tgttgcattt tcaggtgtga tataaccttg tgcaacggtt atatgtgcaa
     3661 acatttttcg gtagtcgtca aatgtacctt caccaagtga gtgatgtaca tatttaccgg
     3721 catgttcaac agcacgtgtc ggcgcacctg taatcgcaat gacaggtatg cgttcagcat
     3781 atgaacctgc gataccgttg acggcactta attcgccaac accaaatgta gtaactaatg
     3841 cagcgagtcc attaagacgg gcataaccgt ccgctgcgta acttgcgttt aattcatttg
     3901 tatttcctac ccaatctaca ttgggattgc tgataatatc gtctagaaaa gcgagattaa
     3961 aatcaccagg aacaccaaaa attttatcga cgcctgctcg atgaatagcg tcaattaagt
     4021 aagctccaat gcgttgtttc ataaaaaaac caccttttct ctatatttac acttaaaatg
     4081 atagcctcga atatagtgta aggtcaagga agttgacttt gttgtgaaaa cgctaacaat
     4141 agtgaacaat taatattttt taggaaatga gtatgaaggg gggctttaat tgatgatctt
     4201 aaaattcaaa atttaagggg agagcgaact atcaatgatg ctgcaaaata caatagcaaa
     4261 gaaaaaacag gcgcgtcacc ttatgaaggt atacgcacct gtttatagta agcattattt
     4321 agcttcaaat aattgatcgc caaatgaaat gttgccatgt tcaccttgtt taaaatcaag
     4381 gtttgtaatg tttccttgtg tcacgataat aggcgtaata tcactctttg catgattgcg
     4441 gatgtagtct aaatcaaagt tgattaataa atcaccttgt ttaacttctt gaccttcctc
     4501 aacatgtaaa gtaaagcctt ctccgtttaa tttaacagtg tctaaaccga tgtggattaa
     4561 taattctaaa ccactatctg atacaagacc aattgcatgt tttgttggga aaatcatttg
     4621 tactttaccg ttgaatggtg cacgaacttc accttgtgaa ggtttgatag cgataccgtc
     4681 acccatcatt ttttcgctga acacttgatc aggcacttct gataatggtg ttacttcacc
     4741 agttaatggt gcatgcacga tatggctcaa ttcgcttgtt gcagatttat cttctgcaac
     4801 aacaacagtt tcgtctttat cgtcttccat agtagtagga ttttctacta cttgaccatt
     4861 cataatctgt tgcatttcat gtttgatttg gtcagattta ggaccaaaaa ttgcttgcat
     4921 attattgccg acttctaata caccagatgc gcctaaatct ttcaaaccag gaacatcaac
     4981 tttagatttg tcgttaactt caacacgtag acgtgtgata caagcgtcta aatgtttaat
     5041 gtttgctttg ccacccatag cttctaatac tgcatatggt aattcagttg ctgaagcagt
     5101 agccgcttgt gattgtttat cttcacgacc tggtgttttg tattttaatt ttacaattaa
     5161 gaatcggaat acgaagtagt aaataactgc gtatacaaga cctacaggaa taactaacca
     5221 ccattgtgtc ttattaggta gtataccgag taagaagtag tcgatgaaac cacctgagaa
     5281 tgtataacct agatgaagat ctaataagta caatgttaag aatgataaac catcaagtac
     5341 tgcgtgaata aagaataata atggtgctac aaataagaat gagaattcta atggttctgt
     5401 aataccagtt aagaatgatg ttaaagcagc agaacccatt aaacctgcta ctactttctt
     5461 attttcaggt ttagctgtgt gataaattgc taaagctgct gcaggtaaac cgaacatcat
     5521 aacagggaat tcaccttgca tgaatttacc agctgtcaaa tgtgcgcctt cacgaatttg
     5581 ttcgataaag atacgttggt caccgtgaat aatttcacca gctgcatttt tccatgaacc
     5641 aaactcgaac cagaacggtg cgtggaaaat gtgatgtaga ccgaatggaa ttaataaacg
     5701 cttgatgaaa ccaaataaga atacggcaac accagtattt gaatctaata atcctgtact
     5761 gaatgcattt aatcctgatt gaatcgttgg ccaaattaat gccattggga atgctaaaat
     5821 aaatgatgtt gtagccatca taataggtac gaaacgctta ccagcgaaga aacctaaata
     5881 agatggtaag ttaatgttat agaacttgtt ataacaccaa gctgccaggg ccccgattat
     5941 aataccgccg aacacacctg tttgtaatgt tgggatacct aaaatgctag cgtaaccact
     6001 cgctggatca ccaatattct taggtgtaac ttgtaaaaag tcgcccattg ttttgttcat
     6061 gattatgtaa ccgacgaatg ctgcgatagc tgctacgcca tcaccgccag ctaatccgat
     6121 tgcgacacct aatgcgaaaa tcataggcaa gttatcaaaa atgataccac cagcacctgt
     6181 cattaattta gcgacagttt gtacgccacc attttgtata aacggcaagt agtgttgtaa
     6241 tgattcacct tgcatagctg taccgatagc taataacaga cccgctgctg gtaaaattgc
     6301 aacaggtaac attagcgctt taccaatacg ttgcaattga ccgaaaagtt tcttcctcac
     6361 ttgtccaacc tccaaagttg tatgtaatat ttaagattca ataaaaaaga cacgagcaaa
     6421 tagactgtgt tggtaaccat cacagcttaa tttaactcat gcctaatctt acttagtaac
     6481 acgttggtgt atgtaattaa tgtaaaagaa gcaattgact aagtagtata taaaactgat
     6541 acgttagttt atctagctta ccatcacatc ttattgaatc ttatttgttt gcgactccta
     6601 ttttagcatg gtgaagtatg cgttttcaat acaattatta aattcaattt caaatctctt
     6661 aattattcac agattattat taaaacataa tataagataa tcctgaataa aatgtataaa
     6721 agttgttgta ataatccagc agtagcagct attatcagca cttaaaagaa aggataatct
     6781 ttgatggtgt aataattaac ttgtgacaga aatatgtaag attattacaa tgaacaggct
     6841 ttaactgatt aatattgtaa tgtgttttct cttatagtaa aatgtaagcg attacacaac
     6901 tcaactatat tttcctaata attatattgt taaggagggc tactttgaca ggcttttcag
     6961 tgtatttagg acaaccttta gatgaagcgt atattaagcg aatgattaaa caaggttacc
     7021 aaatgatttt tacatctgta caaataccag aagaagatga cgagacaaaa tatcattatt
     7081 tcacaaaact actcaattta ttaaaacatg aacaagtgac ttacctcata gatgctaatc
     7141 catctatatt aacaccatct ttttatgagc atcttcgaca atatgatgcg caatttatga
     7201 ttcgtatcga tcatagtaca tcaattgagg caatcgaagc gataatggca cagggtttaa
     7261 agtgctgttt gaatgcaagt attatttccc gggaattgtt aacaagctta catcaacaat
     7321 tgaatgattt tacattactt tcattttgtc ataactatta tccaagacca gatacgggat
     7381 tatctgttga cttggtcaat aagaaaaatg aactcattta tcaatttaat ccaaaggcac
     7441 aaatatatgg ttttattgta gggagtgatt tgcgaggtcc tttgcataaa ggcttgccaa
     7501 caattgaagc aacgagacat agtcatcctg tcgttgcagc taaattatta caagaaactg
     7561 gtgtatctga agtgttagtt ggagactcat tgattgaaat gaggcaggca aaacaactta
     7621 tagatttttg caagcatagg catttcacgt tatgtattga agaagtgttt gatacgacag
     7681 tgacttacct tttcgatatg tgtcataaag tacgcccgga taatccggaa aatgtcattc
     7741 gttcggaaac gtcaagacaa atatgtccac attcgattca accacagttt acgacgcaac
     7801 gacgcattgg ttcagtaacc gttgataatt tgaataacgg acgttatcaa ggcgaaatgc
     7861 aaattgtgag acaaacgctt agtgcacatg acaatgtgaa tgttgttgca caaattatta
     7921 aagaagactt accactgtta agttgtatcg agccgaatga tacatttgat tttcaaaaaa
     7981 ctagggagtg taagaagtga tggaaaatag tacgaccgaa gcgcgtaatg aagcgacgat
     8041 gcatcttgat gaaatgactg tggaagaggg tttaattacg atgaataaag aagatcagca
     8101 agtcccgtta gcagttcgaa aggcaatacc acaattgaca aaagtaatta aaaaaacaat
     8161 tgcacagtat aaaaagggtg gacgattgat ttatatcggt gcaggtacaa gtggaaggtt
     8221 gggtgtctta gatgcagcgg agtgtgtacc tacattcaat actgaccctc atgaaattat
     8281 aggtattatt gctggtggac aacatgctat gacgatggct gtagaaggtg cggaagatca
     8341 caaaaaatta gcggaagaag atttgaaaaa tatagattta acatcaaaag atgtcgttat
     8401 aggaattgcc gcgagtggca aaacgccata tgttataggc ggtttaacat ttgctaacac
     8461 aatcggtgct acaacagtat ctatttcatg caatgaacat gcagttataa gtgaaattgc
     8521 gcagtatcca gtagaagtta aagttggtcc agaagtatta actggttcaa cacgtttaaa
     8581 gtctggtaca gcacaaaagt taattttaaa tatgatttca accatcacaa tggttggtgt
     8641 cggaaaagtt tacgataacc tcatgattga tgttaaagca accaatcaaa aactgatcga
     8701 ccgttcagtg cgtattattc aagaaatatg tgctatcaca tatgatgaag caatggcgtt
     8761 atatcaggta tctgagcatg atgtgaaagt tgcgacagtt atgggtatgt gtggcatttc
     8821 taaggaagaa gcaacaagac ggttattaaa caatggtgac attgttaaac gagcaatcag
     8881 agatagacaa ccttaggagg gatttaaatg accaaagaac aacaacttgc agaacgaatt
     8941 attgctgcag taggtggtat ggataatata gatagtgtca tgaactgtat gacacgtgtg
     9001 cgtattaaag tattagatga gaataaagta gatgaccaag aactaaggca tattgatggt
     9061 gtcatgggtg ttatacacga tgaacgcatt caagttgtgg ttggacctgg tacagtcaat
     9121 aaagtggcta atcatatggc ggaattaagt ggtgttaaac taggtgaccc aataccacac
     9181 catcacaatg atagtgaaaa aatggactat aaatcatatg cagctgataa agcaaaggcg
     9241 aataaggaag cgcataaagc aaaacaaaag aatggtaagt tgaataaagt attgaaatca
     9301 attgccaata tctttatacc gttgattcct gcatttattg gagctggatt aattggtggt
     9361 attgcagcag tactgagtaa cttaatggtg gcaggctata tttcaggtgc ttggattacg
     9421 caacttataa cagtatttaa tgtcattaaa gacggtatgt tagcatactt agctattttc
     9481 actggtatta atgcggctaa agaatttggt gcgacaccag gacttggtgg cgtgattggt
     9541 ggtacaacgt tattaacggg tattgctggt aaaaatattt taatgaatgt cttcactgga
     9601 gaaccattgc aacctggaca aggtgggatt attggcgtta tttttgccgt ttggatttta
     9661 agtattgtcg aaaagagatt acataaaatt gtgccaaatg cgattgatat tattgtaacg
     9721 ccgactattg cattgttgat tgtaggacta ttaactatct ttatctttat gccattagca
     9781 ggttttgttt cagacagttt agtttcagta gttaacggaa ttattagtat tggtggcgta
     9841 tttagtggat ttatcattgg tgcaagcttc ctaccgttag ttatgttagg gcttcatcat
     9901 atttttacgc caattcatat agaaatgatt aaccaatcag gtgctactta cttattgcca
     9961 attgcagcga tggctggtgc tggacaagta ggtgccgcat tagcactttg ggtaagatgt
    10021 aaacgcaaca caacattacg taatacttta aaaggtgcat tgccagttgg tttcctaggt
    10081 atcggagaac cattaatcta tggtgtgact ttgccattag gtcgaccttt cttaactgct
    10141 tgtattggtg gtggtattgg tggcgctgta ataggtggaa ttggacatat tggtgccaaa
    10201 gcaataggcc caagtggtgt gtcactatta ccattaatct cagataatat gtatttaggt
    10261 tatattgcag gattacttgc tgcgtatgct ggtggattcg tttgtacata tttatttgga
    10321 acgacaaagg cgatgcgaca gacagatttg ttgggtgatt aatgatgaca aatattttat
    10381 atcgcattga taagcagttg agtgatttta cgaagacaga aaagataatc gctgattaca
    10441 ttttaaagaa tccacataaa atcattgata tgactgtgaa tgatttggca gatgttacga
    10501 atgttagtac agcatcaatt gttagattta gtcggaaaat gacacatcaa ggttttcaag
    10561 agctaaagat tgcgatatct cgatacttac ccgaagatat tgcaaccaat ccacatttag
    10621 aattgattga aaatgaatct gtagaaactt tgaaaaataa aatgattgct agagcaacga
    10681 atacgatgcg atttgtagct actaatatta tggatgcgca aattgatgca atttgtgatg
    10741 tgttgaaaaa tgccaggaca atatttttat ttggatttgg cgcatcgagt ttgactattg
    10801 gtgatctttt tcaaaagtta tctcgtattg gcttaaatgt caggttatta catgaaacgc
    10861 atttacttgt gtcaacattt gcgacgcatg atgatagaga ttgcatgatt tttgtgacga
    10921 atcaaggtag tcatagtgaa ttgcagtcaa ttgcacaggt ggccacacat tacagtattc
    10981 ccatcataac tatatctagt acagctaata atccagtggc tcaaattgca gactatgcat
    11041 tgatttatgg cagaactgat gaaaatgaaa tgcgtatggc ggctacaacg tcactatttg
    11101 cacagttatt cacggtagat atattgtact atcgatttgt agcattaaat tatcatgcga
    11161 ttctagattg tataacccaa tcgaaaatgg cacttgataa ttacaggaag catcttgcga
    11221 cgatagattt taaacattag atattgtgtg cgttctaaca taaagttagt tgcaacgtaa
    11281 acaattgaag tttttgatgt gattcagttg cttgatgaaa aattgttgct acaagtttgt
    11341 aaaactggca tagaggctat tttgaaaatg ataaaggcgt gacaaattaa agcaaagttc
    11401 agatttgagc tttgcttttt tcatagatgg aaattaaatg ctgaagaata attttaatgc
    11461 taaatgaact aatacgaagg tgctaaaagc aattattatc aaattagtga tatgaatgaa
    11521 atgtttaaat cgtttgcgtt gattgtattc gggactttta tagagtaggt agtagtacat
    11581 aaagagtatg gctgcgaaca tgccgaatat tgaagtgaag ttacctacag tgactgccat
    11641 gtaatgattt aaagttaaat agtaacttag actaataata aaaaataaaa tgatttgata
    11701 ggtaattaac accattcaag tccctccatt aatcgtagac aaaaaattta tcaatgattt
    11761 gattatttgt attcaaattt tagtatacga cttacctcaa agacaaatat taaaagataa
    11821 gacaataaaa ttagaataaa tttacgtttg aagataaaaa acaaacattt ttcattaaag
    11881 tttatgatat atttagagca atagaaagtg tatggaaggg gatatgaatg gcataccaaa
    11941 gtgaatacgc attagaaaat gaaatgatga atcaacttga acaattgggt tacgaaagag
    12001 taacgatacg tgataataag caattgcttg ataattttag aacgatttta aatgagcgtc
    12061 atgcggacaa attagaaggc aatcccttaa cagataaaga atttcaacgt ctgttaacga
    12121 tgattgatgg aaaaagtatt ttcgagagtg cccgtatttt acgtgataaa ttaccactta
    12181 gacgtgatga tgagtctgag gtttatttgt cgtttttaga tacgaaaagt tggtgtaaaa
    12241 ataagtttca agtgacgaat caagtatctg tcgaggatac atataaagca cgttatgatg
    12301 taacgatatt aatcaacgga ctaccccttg tccaagttga attgaaacgt cgaggtattg
    12361 atattaatga ggcgtttaac caagtaaaac gttaccgcaa acaaaattac acaggcttat
    12421 tccgctacat acaaatgttt atcattagta atggtgttga aacgcgatac ttttctaata
    12481 atgatagcga actattgaag agtcacatgt tttattggag tgataaacag aataaccgta
    12541 tcaatacatt gcaatcgttt gctgagtcat ttatgagacc ttgtcaatta gctaagatga
    12601 tatcgcgcta tatgattatt aatgaaacag atagaatact gatggcaatg cgtccgtatc
    12661 aagtgtatgc ggtagaagca cttattcaac aagcgactga gacagggaat aatggatatg
    12721 tatggcatac aactggaagt ggtaagacgt tgacttcttt taaagcgagt cagattttat
    12781 cacagcaaga tgacattaag aaagttatct ttttggttga ccgtaaagac ttggatagtc
    12841 aaacagaaga ggaatttaat aaatttgcta agggtgctgt agacaaaact tttaatacct
    12901 cgcaactggt acgccaacta aatgataaaa gtttgccact tattgtaacg acgattcaaa
    12961 aaatggctaa agcgattcaa gggaatgccc atttattaga acagtataaa acgaataaag
    13021 ttgtatttat tattgatgag tgtcatcgca gtcaatttgg tgacatgcat cgtctagtta
    13081 aacaacattt caaaaatgcc caatactttg gattcactgg tacgccacgt tttccagaaa
    13141 atagtagtca agatggtaga acaactgcag atattttcgg tagatgctta catacgtatt
    13201 taattagaga tgccattcat gatggtaatg tacttggttt ctcagttgac tatattaata
    13261 cttttaaaaa taaagcttta aaagcagaag ataacagcat ggttgaagca attgatacgg
    13321 aagaagtatg gttagcggat aaacgtgtgg aattagtaac acgacatatc atcaataatc
    13381 atgataaata tacacgtaat cgtcaatatt caagtatatt tacagtccaa agtattcacg
    13441 cgcttattaa atattatgag acatttaagc gacttaacaa aaagttggaa caaccgttaa
    13501 cgatagctgg tatatttacg tttaaaccta atgaagatga tcgtgatggt gaagtgccat
    13561 atcattcacg tgaaaaatta gagataatga ttagtgatta taataaaaag ttcgagacga
    13621 atttttcaac agacacaact aatgagtatt ttaatcatat ttcaaaaaac gttaaaaagg
    13681 gcgttaaaga tagtaaaatt gatatcttaa tcgttgttaa tatgttctta actggttttg
    13741 atagtaaagt actgaacact ttatatgttg ataagaattt aatgtatcat gatttaattc
    13801 aagcgtattc acgtacaaat agggttgaaa aagaatcaaa gccatttggt aaaattgtaa
    13861 actatcgtga cttgaaaaaa gagacagacg atgcactgag agtattctca caaacaaatg
    13921 atacggatac aattttaatg cgcagttatg aagagtataa aaaagaattt atggacgctt
    13981 atcgtgagct taaaatgatt gtgccgacac cacacatggt tgatgacatt caagatgaag
    14041 aagagctaaa gcgctttgtt gaagcttatc gtttattagc taaaataata ttacgtttaa
    14101 aagcatttga cgagtttgag tttacaattg atgaaattgg aatggatgaa caagagaatg
    14161 aagactataa aagtaaatat ttagctgtgt acgatcaagt aaaaagagcg acggctgaga
    14221 aaaataaagt atccatttta aatgatattg atttcgaaat agaaatgatg cgtaatgata
    14281 cgattaatgt gaattatatt atgaatatat tgagacaaat tgatcttgaa gacaaagcgg
    14341 aacaacgtcg taaccaagaa caaattagac gcattttaga tcatgcagat gatccgacat
    14401 tgaggttaaa acgagatcta attagagaat tcatcgacaa tgttgtacct tctttaaata
    14461 aggatgatga tatcgatcaa gaatatgtta atttcgaaag tattaaaaaa gaagcggagt
    14521 tcaaaggatt tgctggagag agatctatcg atgaacaagc cctaaaaaca atttcaaatg
    14581 actaccagta tagtggtgtt gtaaacccac atcaccttaa aaaaatgatt ggtgatttgc
    14641 cattgaaaga aaagcgtaaa gcaagaaaag ccattgaatc tttcgtggca gaaacaactg
    14701 aaaaatacgg tgtgtaatga ttcagccccc tcgctagatt agtgtagggg gcattattat
    14761 ttcattagaa aaggctcatt tgttaaattc atcttatcaa gaatataacg aatgttcctt
    14821 gttttaaact tattaattaa tagagacatt ttatctgaaa atatcactga atgctaatac
    14881 taatcaaaga aaaagtgata attaaaataa gatatataat accccaaata gtggatgctc
    14941 gtatagaaag tttcgacgta ccaattagca tgactgtaag tatgtatgct tgtacaaaaa
    15001 attgtaagtc gaaagtaccc agaatgcgat ttcctttagc aaaaatattt aaactagtaa
    15061 tagaatagtc aattaatgat aaaccggcac ttatttggat ggtaagtata attaaactaa
    15121 tagcatttgt aaacagtatg tagcttaacg ttgcagagaa tatagatttt gtattagcat
    15181 ctgagcgcat aattctagag ataactagaa tgattaaaaa tgtaaataat acgctgaaaa
    15241 ctcttgagaa aaaaatacta atttgactaa aaatttttat ttgtgctaat gatgtctctg
    15301 agagtcctgc ttcactcaat aactttttat agtccacaga tagtccacta acaaatgtat
    15361 atgtaacaat tattatcaaa gtcagaaaca gcttaatgat taactttggt tgaatgcgga
    15421 tatttgcaaa gtgattaagc aaaggtagtt ttgataatga catatagacc tcctaatgat
    15481 ataattctcc tatattttat cataaaaata atataaaaaa tatattttat taaaaataag
    15541 tataaaaaat ggtttgaaga gtatttaaca tttataggag gacataaatt gaggcgtaaa
    15601 aggaaatggc taattgttat ttgtgtatta ttagtattga ttacgatatc acaaattttg
    15661 aaacatttta actatggaac tgagagtaaa gaagcataca atacatatac ggttgaaaaa
    15721 gaaagacaat tagatttaca aggtaaggca tatccacgtg aagttaaaac atattataaa
    15781 aataatcagg ttggaacata tttgggtgtg caagtagcgg acgggcaaac agtcaaaaaa
    15841 ggagacaaag ttaacttaag tattacgagt acgggtaaaa caggagcggg gattataaaa
    15901 caaatctctg agttgcctat tagttatgaa gagaatttaa gtcttcacga aaatgatgcc
    15961 ctgcatttac cagaaagtaa caacgatggg gaattagcaa attcaaaatc gatttcggca
    16021 tctccaatat ttaagagtaa taacaactct gaattatcaa agtatggagt tataattgat
    16081 gatcttagtc taccaatacg tgcaggttat tcgttggaaa ttaaagtccc attaaatgcg
    16141 attaaaatac ctaaatcagt attaacaaaa ggcaataatg tttttattgt taataaaaat
    16201 agtattgtgg aaaaacggaa tattcgtata aaaagaatca atggtgacat tattgtagaa
    16261 aaggggttaa aacctggaga caagttaatt aaacaaccta aaagtacaat gaatgatggt
    16321 gataaggttg agatttcgtc atgatagaat taaagaatgt ttataggact tttaaaaacg
    16381 gtgataaaaa tattcgagtt ttaaaagaca ttaatataca tatcaatgca ggtgaattca
    16441 tagcgattat gggaccatca ggctcaggga agagcacact gattaatatc ttaggattta
    16501 tagatcgaga gtatcatggt gaatatttgt ttgaaggaag taattatcag acacaatctg
    16561 ataatcaatt ggcaaacata agaaataaaa cagttggatt tgtttttcaa aattttaaac
    16621 taattcataa taatactatt ttagaaaata taagtatgcc actggtatat acaaatatga
    16681 atacgataga acgaaagaat aaagttatga ctgtgctcag tgctgtaggc ctagatggca
    16741 aaggtgattt tactcctagt aaactatcag gcggtcaaca acaacgtgtt gcaattgcgc
    16801 gagccattat aaataatcca aagtttatta ttgcagatga gccaacgggt gctttggatt
    16861 cagatacatc taaaattatt atggacatat ttacatcatt aaaccgagat aatcatacta
    16921 caattatttt agtcacacat gatcgtaaag tagcagaaaa agctgatcga ataattcata
    16981 ttttggatgg tcgtatacaa catgaagagg tgattaaatg aggaaagttt taaatatttt
    17041 tacaatagct tttaaatcaa tattgaaaaa taaaggtaga aatatattta caatgatagg
    17101 tataatcatc ggtatttctt ctgttattac gataatgtct ttgggaaatg gctttaaaaa
    17161 aactgctgct gatcaatttt cagatgctgg cgccggaaaa caagaagcgt tgattagctt
    17221 tacttttaaa gttgatgaga aaatcaaaaa gtatcctttt aatcaaagag atatagagtt
    17281 ggtaaatcaa gttgatggcg tactagatgc gaagctaaag gaaaataaag aagaaggaat
    17341 tgaagccaca ataactaatg tccagaaaaa aagtgacatc ttcattataa aaaaacaaaa
    17401 tttacactct tttaaagtag gtagagggtt tgataaagag gataatgaat tacgtaagaa
    17461 aatagtggtt ataaatgatc aagtagcaaa aactgtattc aataataatg ctattggtaa
    17521 atcgctatat attgagggac agggatttga agtcatagga attacggata aactatattc
    17581 tgactcatct accgttataa tgcctgaaaa tacattcaac tactatatgg gacacttaca
    17641 tcaaggtctg cctaccttac agataattat tgaagatggt tacaataaaa aaactgtagt
    17701 taaaaaagta gaatcactat taaataaaaa aggatcgggc tctgttttag gtgagtatac
    17761 atatacagac actgaagaaa ttatcaaaag tattgataaa atttttgata gtattactta
    17821 ttttgtagca gctgttgccg gtatttcact ttttattgct ggtattggag ttatgaatgt
    17881 catgtatata tctgtagctg aaagaacaga agaaattgcg atacgacgtg catttggtgc
    17941 aaaaagtcga gatattgaac tacaattttt aatagagagc attttaatat gtgtgacaag
    18001 tggttttatt ggacttattt taggtgttgt gtttgcaaca ataattgatg tattaacacc
    18061 agattatatt aaaagtgtag taagtttgag ttctgtcata attgcggtta gtgtgtcgat
    18121 attaattgga cttctttttg gatggatacc agctcgcgca gcatctaaga aagagctcat
    18181 agatattata aaatagtaaa tttaaaatgg taaaagtact gaagactaat tat
//
LOCUS       DBNENW010000043        18021 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNENW010000043 DBNENW010000000
VERSION     DBNENW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.043"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            31..828
                     /locus_tag="AC1LW8_001156"
     CDS             31..828
                     /locus_tag="AC1LW8_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773003.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            864..1868
                     /locus_tag="AC1LW8_001157"
     CDS             864..1868
                     /locus_tag="AC1LW8_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP4773004.1"
                     /translation="MTNRENSTPLKFLAYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNSLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            1865..2881
                     /locus_tag="AC1LW8_001158"
     CDS             1865..2881
                     /locus_tag="AC1LW8_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP4773005.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGIATALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            3115..4083
                     /gene="dhaK"
                     /locus_tag="AC1LW8_001159"
     CDS             3115..4083
                     /gene="dhaK"
                     /locus_tag="AC1LW8_001159"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIP4773006.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            4125..4709
                     /gene="dhaL"
                     /locus_tag="AC1LW8_001160"
     CDS             4125..4709
                     /gene="dhaL"
                     /locus_tag="AC1LW8_001160"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIP4773007.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            4702..5064
                     /gene="dhaM"
                     /locus_tag="AC1LW8_001161"
     CDS             4702..5064
                     /gene="dhaM"
                     /locus_tag="AC1LW8_001161"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIP4773008.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            5180..5677
                     /locus_tag="AC1LW8_001162"
     CDS             5180..5677
                     /locus_tag="AC1LW8_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773009.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            6134..7201
                     /locus_tag="AC1LW8_001163"
     CDS             6134..7201
                     /locus_tag="AC1LW8_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvI family membrane protein"
                     /protein_id="HIP4773010.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            7352..8395
                     /locus_tag="AC1LW8_001164"
     CDS             7352..8395
                     /locus_tag="AC1LW8_001164"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4773011.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            8724..9152
                     /locus_tag="AC1LW8_001165"
     CDS             8724..9152
                     /locus_tag="AC1LW8_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773012.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(9361..9867)
                     /locus_tag="AC1LW8_001166"
     CDS             complement(9361..9867)
                     /locus_tag="AC1LW8_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP4773013.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            9980..10903
                     /gene="graX"
                     /locus_tag="AC1LW8_001167"
                     /gene_synonym="apsX"
     CDS             9980..10903
                     /gene="graX"
                     /locus_tag="AC1LW8_001167"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HIP4773014.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            10919..11593
                     /gene="graR"
                     /locus_tag="AC1LW8_001168"
                     /gene_synonym="apsR"
     CDS             10919..11593
                     /gene="graR"
                     /locus_tag="AC1LW8_001168"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HIP4773015.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            11586..12626
                     /gene="graS"
                     /locus_tag="AC1LW8_001169"
                     /gene_synonym="apsS"
     CDS             11586..12626
                     /gene="graS"
                     /locus_tag="AC1LW8_001169"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HIP4773016.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            12770..13531
                     /gene="vraF"
                     /locus_tag="AC1LW8_001170"
     CDS             12770..13531
                     /gene="vraF"
                     /locus_tag="AC1LW8_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HIP4773017.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            13521..15410
                     /gene="vraG"
                     /locus_tag="AC1LW8_001171"
     CDS             13521..15410
                     /gene="vraG"
                     /locus_tag="AC1LW8_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HIP4773018.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAVIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            16135..16752
                     /locus_tag="AC1LW8_001172"
     CDS             16135..16752
                     /locus_tag="AC1LW8_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIP4773019.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            16768..17775
                     /locus_tag="AC1LW8_001173"
     CDS             16768..17775
                     /locus_tag="AC1LW8_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIP4773020.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
BASE COUNT         6341 a         2402 c         3301 g         5977 t
ORIGIN      
        1 agaaagaatt ttattgaaag tagggaaatt atgaatcgtt tgcatggaca acaagttaaa
       61 attggttacg gggataacac gattataaat aaattagatg ttgaaatacc agatggcaaa
      121 gtgacgtcaa tcattggtcc taacggctgc gggaaatcta ctttgctaaa ggcattgtca
      181 cgtttattgg cagttaaaga aggcgaagta tttttagatg gtgaaaatat tcatacacaa
      241 tctacgaaag agattgcaaa aaaaatagcc attttacctc aatcacctga agtagcagat
      301 ggcttaactg ttggggaatt agtttcatat ggtcgttttc cacatcaaaa aggatttggt
      361 agattaactg ctgaggataa gaaagaaatt gattgggcaa tggaagttac aggaactgat
      421 acattccgac accgttcaat caatgattta agtggtggtc aaagacaacg tgtttggatt
      481 gcaatggcat tagcacaaag aactgatatt atctttttag acgaaccaac aacatattta
      541 gatatctgtc atcaattaga aatactagaa ttagttcaga agctaaatca ggaacaaggt
      601 tgtacaattg tcatggttct tcatgatatc aaccaagcga ttcgtttctc agatcatctt
      661 attgcgatga aagaagggga tatcatcgct acaggttcaa cagaagacgt attaacacag
      721 gaaattttag aaaaagtttt caatattgat gttgttttaa gtaaagatcc taaaactgga
      781 aaacctttat tggtaactta tgacttatgt cgcagagctt attcttaatt aagtaagtta
      841 atatgataaa aaggacaatt aacatgacaa atagagagaa ttcaacgcca ttgaagtttt
      901 tagcctatat tataggttta agtatgatac tactaatcac actatttatt tctacattaa
      961 taggtgacgc caaaattcaa gcctctacaa ttatagaggc tatttttaat tataatccta
     1021 gcaatcaaca gcaaaacatc atcaatgaga ttaggattcc cagaaatata gcagcagtaa
     1081 ttgtgggtat ggcgcttgca gtttctggtg cgattataca aggtgttacc cgtaatagtc
     1141 ttgctgatcc ggcgctcata ggtttaaatt caggtgcttc atttgcttta gcattaacat
     1201 atgcagtttt accaaacact tcatttttaa tattgatgtt tgctggattt ttaggtgcta
     1261 ttctaggagg tgctattgta ttaatgatag gccgatctag acgtgatgga tttaatccga
     1321 tgcgtattat tttagcgggt gcagcagtaa gtgctatgtt aacagcgcta agtcaaggta
     1381 ttgcattagc ttttagacta aatcaaacag taacattttg gactgctgga ggcgtttcag
     1441 gcacaacatg gtcacacctt aagtgggcaa ttccattaat tggtattgcg ttattcatta
     1501 tattaacaat tagtaaacaa cttaccattt taaatcttgg tgaatcatta gctaaaggtt
     1561 taggtcaaaa tgtaacaatg atcagaggca tatgtttaat tattgctatg attctagcag
     1621 gtattgcagt tgctatcgct ggacaagttg catttgtagg tttgatggta cctcatatag
     1681 caagattttt aattggaact gattatgcta aaattctacc attaacagcc ttgttaggtg
     1741 ggatactcgt gcttgttgcc gatgtgatag cacgatattt aggagaagcg cctgttggtg
     1801 caatcatttc atttatcggt gttccttact ttttatattt agttaaaaaa ggaggacgct
     1861 caatatgatt agttcaaata ataaacgcag acaatggatt gcactggctg tttttagcat
     1921 tctactattt ctaggttgta cttggagtat tacctcaggt gaatacaaca tacctgttga
     1981 aagatttttc aaaactttaa ttggacaagg tgatgccatt gatgagttaa tcttattaga
     2041 tttcagatta cctcggatga tgattactat tttggctggc gcagcgctta gtattagtgg
     2101 tgcaatagtg caaagtgtca cgaaaaatcc aatagctgaa cccggtatat taggtattaa
     2161 cgcaggtggc ggatttgcaa tcgcattatt tattgcaatt ggtaaaatta atgctgacaa
     2221 ctttgtttat gtactgccgt taataagtat actaggtggt atcgccactg cattgattat
     2281 ttttattttc agttttaata aaaatgaagg tgttacacct gcgagtatgg tattaatagg
     2341 tgtaggttta caaacagcat tatatggtgg ctcaattaca attatgtcaa aatttgatga
     2401 taagcaatct gatttcatcg ctgcttggtt tgcaggtaat atttggggtg acgaatggcc
     2461 atttgtcatt gcatttttac cgtgggtgtt gattattatt ccttacttac tatttaaatc
     2521 gaatacacta aatattattc atacgggtga taatattgca cgaggtctag gtgtaaggtt
     2581 aagcagagaa cgtttaatat tattctttat cgcagtgatg ttatcatctg ctgctgtagc
     2641 agtagcaggt tcgatttcgt ttatcggatt aatgggtccg catattgcca aacgtatcgt
     2701 tggaccacgt caccagttgt ttttaccaat tgccatttta gtaggggcat gtttacttgt
     2761 tatagctgat acaattggca aaattgtatt acaaccaggt ggggttccag caggtattgt
     2821 cgtagcaatt attggtgcac cgtatttctt atatttaatg tacaaaacga aaaatgtata
     2881 gtgtcaaagg acacaactta ttgctatgaa aggcacttta ttataaggct tttcatagca
     2941 ttttttattt aatgagccac tcaagactat ttattttttc aataatgaac cattaagtta
     3001 tcaagaggat cttatcaaaa atatatttga taacggtatc aggttaattc tttatgatag
     3061 cgcattcatt tattctgttt tatactatga ctgataatac caaggaggta caacatgatg
     3121 aaaaagttaa tcaataaaaa agaaacattt ttaactgata tgcttgaagg attgttaatt
     3181 gcgcacccag agttagatct gattgctaat acagttattg taaaaaaagc taagaaagaa
     3241 catggtgtag caatagtctc tggaggtgga agcggacatg aacctgcgca tgccggtttt
     3301 gttgcagaag gtatgctaga tgcagcggtt tgtggcgaag tatttacatc acctacacct
     3361 gataaaatat tagaagctat taaagcagta gatactggtg atggtgtatt actagttgta
     3421 aaaaactatg caggtgacgt gatgaatttc gaaatggcac aagagcttgc agaaatggaa
     3481 ggtataaatg ttcaaactgt tattgttcgt gacgacattg ctgtgacaaa cgaagtacaa
     3541 cgtcgtggtg ttgcaggaac agtgtttgtt cataagcttg ccggttatct tgctgaaaaa
     3601 ggttattcat taacagagat aaaatcgcgt gtagaagcgt tgttacctga aattaaaagt
     3661 attggtatgg caattgagcc accgcttgtt ccaactactg gaaaatatgg ctttgatatt
     3721 gaagacgaca aaatggaaat cggtattggt atacatggtg aaaaaggtat tcatagggaa
     3781 gaagtaaagg atattgatca tattgttgga acattgttag acgaattgta taaagaagtt
     3841 actgccaatg atgtcatatt aatggtaaat ggtatgggtg gtacgccgtt atctgaatta
     3901 aatatcgtaa ctaaatatat tcaacaaaat ttagctgcaa gaacggttaa tgttgctaaa
     3961 tggtttgttg gtgattatat gacatcttta gacatgcaag gtttttctat aactatcgtg
     4021 cctaataaac cagaatattt ggaagcattt ttagcaccaa caacaagtca atactttaaa
     4081 taagaagtga atatgaataa atatacattt atgaggtggc acaaatgaaa gtgaatgata
     4141 tgaaagcacg tttattaaat ttagaagaaa cgtttaaaaa acatgaatct gaattaactg
     4201 aattagatcg agcaattggt gatggtgacc acggggttaa catggttcgt gggtttagta
     4261 gtcttaaaga caaacttgat gatagctcaa tgcaatcatt gttcaaatcg actggtatgg
     4321 cattgatgtc aaatgttggg ggtgcatcag gaccactgta tggctttagc tttgttaaaa
     4381 tgtctgcagt caccaaagat gatatggata atcaagattt cattacacta attcaggcat
     4441 ttgccgaagc ggttgaatca cgtggtaaag ttactttaaa tgaaaagaca atgtatgatg
     4501 tagtagcgcg agcagcagag aagcttgaaa atggtgaaac tttaacattc aatgatttac
     4561 agcaattagc agataataca aaagatatgg tagcaacgaa aggtagagct gcatattttg
     4621 gagaagaatc aaaaggttat attgatccag gtgctcaaag tatggtttat attttaaacg
     4681 ctttgattgg agatgaagat aatgcctaaa attatacttg ttagccacag taaagaaatt
     4741 gcaagtggta caaaatcttt gttaaagcaa atggcaggtg acgttgatat tataccaatc
     4801 gggggattac cagatggttc aattggaact tcatttgata tcatccaaga agttttgact
     4861 aaattagagg atgatgcatt gtgtttttac gatattggat cttcagaaat gaatgtagat
     4921 atggctattg aaatgtatga tggtaatcat cgtgtgttaa aagttgatgc accaattgtt
     4981 gaaggcagtt ttatcgcagc agtaaagcta tcaatcggcg gttcaattga tgatgcatta
     5041 gcagaaatca aacaatcatt ttagttaaaa tttactaata atgaaaaatg taaacctttt
     5101 tcaaatgaaa ctttataaaa aatatgatag tatatatgta aatgtttaat aaaatctgga
     5161 gaaataggag gacattgcca tgcaacacct tataaaaaaa catgtattga atggcgagtt
     5221 tgatttagta cgacaattga tgtccgaaac agattttatg gaatttgaag aagcatatat
     5281 ttcaagtgcg catgaagtag aaagtatgat gttttataca tgtattttag atatgattaa
     5341 gtacgaagaa tcatctgaaa tgcatgactt agcattttta ttgcttgtgt atccactaag
     5401 tgaatatgaa ggtgctttgg attctgctta ttatcatgca gacgcttcca taaaacttac
     5461 tgacggcaaa gaagttaaaa gtttgttaca aatgttatta ttgcatgcga taccaacacc
     5521 tgttatttca gataagaagg cttttgatat cgccaagcaa attttaaaat tagatcctaa
     5581 taataatgtt gctcgtaacg tcttaaaaga cactgccaaa cgtatggaca acgttgttgt
     5641 tgatataaat gaattacacc aacgtaatgc acgttaatta catttcaatt atattagctt
     5701 aataatagtt ttaacatttg gttgggttgg gacatatgtt ccagcctttt ttaatactta
     5761 aaaactaacg aagtatactt gtgtgcacaa atggttttta tacaacattt tataaattta
     5821 tacattttaa taaagaacat acgatagatg gtttaaacct tgttaactga gaaattttga
     5881 tatgtattct tcgaaattta actaaatata cgaaattcaa gaagcacaat aattaatcat
     5941 ttttcctata caaaagttcg tatgactgca ttataaaagc ataaatttat aattttttta
     6001 aatgtcattg aacgtgataa tgtgaatgga ttgagcaatt ttgaaaaagt gaaaaataac
     6061 ctatgcgact tgcaattaat tttcagtacg ttataatgca cactgtgcaa aattaaggag
     6121 gtctattatt cacatgatga tgaataaaga agcaacaaaa attggatttg cctacgtcgg
     6181 cattgtagtg ggcgcaggat tttcaactgg acaagaagtt atgcaatttt tcactaaata
     6241 tggcttgtgg gcttatttag gtgttattat atctggtttt attttagctt ttattgggcg
     6301 ccaagtagca aaaattggta ctgcctttga agcgacaaat catgaatcaa cattacaata
     6361 cgtattcggt gaaaagttta gtaaagtctt tgattatatt ttaatcttct tcttatttgg
     6421 tatagctgta accatgatag ctggtgcagg cgcaacattt gaagaaagtt ataacatacc
     6481 tacatggcta ggtgctttaa ttatgacatt agcgatttat attacgttgc tattagactt
     6541 taataaaata gtacgtgcac taggtatcgt tacaccattt ttaattgttt tagttgtatt
     6601 aatcgctggc gtttatttat ttaaaggtca tgtttcatta gcagaagtta accaagtagt
     6661 gcctgaagca agtatttgga agggaatctg gtttggtaca atatatggtg gattagcttt
     6721 ttctgtaggt tttagtacca tcgtagcaat cggtggggat actgaaaagc gtacagtgtc
     6781 aggtgcaggc gcgatgtatg gtggtattat ctatactgta ttactagcat tgatcaactt
     6841 tgcattgcaa agtgaatatc caactattaa aaatgcctca attcctacat tgacgttagc
     6901 aaataatatc catcctttaa tagcaacagt gttatctgtt attatgctgg cggttatgta
     6961 taatactatt ctaggactaa tgtattcatt tgcagcacgt tttacagaac catacagtaa
     7021 aaattatcat atctttatta ttataatgat ggtagcaggt tatttattaa gtttcgtagg
     7081 atttgctgaa ttaattaata agttatatac aattatggga tatgtaggct tatttattgt
     7141 agtagctgta attattaaat atttcaaacg taaaaatgcg gataaaaaac atattgctta
     7201 atatcatatg agggatatcc gaaactttac aattgaatca ctttgtttta accttaaaag
     7261 caattcgtct ctactcttat cgggcgaatt gctttttata tttattcagt ctattaatat
     7321 gagcgtctaa caaatagaga ggtacgatgt aatgaataaa gataataaat ggacgatgat
     7381 aactgcgctt tttataactg taatcagtgt attgttagca tttcatctga aacaacatta
     7441 tgaccaaatt acaaatgaga accatgctaa taaagacaaa attaatatta aaaataaaaa
     7501 tgtgcgcatt tatcaaaacc ttacatacaa tagagttttc cctaacagta aattagatat
     7561 tattacacct gttgatatgt cttctaatgc caaactgcca gttatttttt ggatgcacgg
     7621 tggtggttat attgcgggtg ataagcagta taaaaaccca ttattagcga aaattgctga
     7681 acaagggtac attgttgtga atgtaaatta tgcattggcg ccacaatata aatatcccac
     7741 accattaatt caaatgaatc aagcaactca attcattaaa gaaaataaaa tgaatttacc
     7801 tattgatttt aatcaagtaa ttattggcgg tgattctgca ggtgctcaat tagctagcca
     7861 atttacggca atacagacga atgatcgctt aagagaagcc atgaaatttg atcagtcatt
     7921 caaaccatcg caaattaaag gtgctatact atttggtggt ttttataata tgcaaacagt
     7981 tagagaaact gagtttccaa gaatacagtt atttatgaaa agttatactg gcgaagaaga
     8041 ttgggaaaag agttttaaaa acatttcaca aatgtcgaca gtaaaacaat cgacaaaaaa
     8101 ttatccacca acatttttat ctgttggaga tagcgatcca ttcgaaagtc aaaatataga
     8161 attcagtaag aaattacaag aattgaatgt accagtagat actttgtttt atgatggtac
     8221 gcatcattta catcatcagt atcaatttca ccttaataaa cctgaatcga tagataatat
     8281 caaaaaagtg ttacttttct taagtcgtaa tacatcttct agtggtattc aaactgaaga
     8341 gaaaccacaa atagaaaatc cgagtaatga attaccgtta aatcctttaa actaatgata
     8401 aacagtagta atttattact taagcaacat ttaagatctt caaattgaaa acgaagaatt
     8461 taaaacatgt ggggctaatg tgtaagagtc tgagatataa taaattgtta gcggttcttt
     8521 atcatttcta tctcacccta ttatgatgtg acatttattt tacaaactaa tttgttttga
     8581 acctgaaaat aactttttac aactaaattg tcgaaaaaca gtgtgtacat gatataataa
     8641 atttataaat tgaaaagaat tcaaaagaaa aaataaaata tagattgagc acgaagtgat
     8701 ttgaaataag gttgtgaaag ggaatgacaa ggtcagcatt aaaaccattt aaaaataaac
     8761 gcgttatggt tactggacgt atacaacgtg ttttgtttaa aaattattta gatagacata
     8821 gcacatttaa gccgaatgta aggatattat taaaagatgt atttgtttca ggtgtatcaa
     8881 tagatcattt atggttatat gagacaaata aatactatgc attggcaatg gaacttattc
     8941 atcaacgagt aaaatttagt gcgaatgttg taccatatta caaaataaat agaaataata
     9001 atttattcgt acaagattat ggaattaagc gtaaaggtag gttaattact gaagaagctt
     9061 acaatcaaaa caatcagtat caggataaga tatatgaaaa attaccggat atagatttta
     9121 gactcgaaga tttttatagt aaggaaaact aaacaacata tataaagcac cactaatcgt
     9181 agttgatagt tgatcaatta cacattagtg gtgctttatt ttgaatttaa atttgataaa
     9241 tgaataagag tatacgcatt catttaagat tagatgacat tgataagtat gagtaatgta
     9301 ttaggtaacc attaaattcg ttgttatgcg attctgatac aagattatga taaaatagct
     9361 ttaattaaga attttagcca tcatatagtc atcgtaatat ttaccatcga taaataactt
     9421 atcttttaaa acgccttcga tttgaaaatc ggcactttta aaaagctcga gggcaggttg
     9481 gttattgagt ggtacatttg cttcaattcg gtgtatttga ttgtttaaac accaagccat
     9541 aatggcatca agaagtgctt ggccaattcc acgatgttga tataatttct ttacacctaa
     9601 atcaatttta gcaacatgtt taatgcgttg aaatggtgtc gtattaacaa aggcaaagcc
     9661 aacgagttgt tcatcacttt cagcaacaaa gatgacttta tgtggagaag tgatatattc
     9721 ttctaattgt ttactagccg acgtgacgct aggatcatat tctcctggtg tgtagaacat
     9781 atacggagat tcgtcgtata ttttcgctaa cattgaaatg aaattttcta catctttgat
     9841 actaactcta cgtataatat gggccataaa aagcctccag tattttgaag ttttaaaaca
     9901 tttgctacta ttataatata tatgtaacta aaaggtggag taatatgttt tttggttaca
     9961 gtttaaagga gtattttaga tgaaacctaa agttttatta gcaggtggaa caggatatat
    10021 tggtaagtat ttaagtgaag tgattgaaaa tgatgctgaa ctttttacta tatcaaaata
    10081 tccagacaat aaaaaaacag atgatgttga aatgacttgg attcagtgtg atatatttca
    10141 ttacgaacag gttgttgcag caatgaatca aatagatatt gctgtattct ttatcgaccc
    10201 aacaaagaat tctgccaaaa taacacaatc atcagcaaga gatttaacat taatcgcagc
    10261 agataatttt ggtcgagcag cggctatcaa tcaagtaaaa aaagtaatct acatacctgg
    10321 gagtcgttat gataatgaaa caattgaacg cctaggtgca tatggcacac ctgtagaaac
    10381 aacaaattta gtttttaaac gttctttagt taatgtagaa ttacaagttt caaagtatga
    10441 tgatgttaga tcaacgatga aggtagtttt accaaaggga tggacattaa agaacgttgt
    10501 aaaccatttt attgcatgga tgggttacac taaaggaact tttgtgaaaa cagaaaaatc
    10561 acatgatcaa tttaagatat atattaagaa taaggtgcga ccgctcgcag tatttaaaat
    10621 agaagaaaca gctgacggaa taataacttt aattttattg agtggaagtt tagtgaaaaa
    10681 atacacagtt aatcaaggga agttagaatt tagattaatc aaagagtcgg cagtcgttta
    10741 tatacatcta tacgattata tccctcgatt attttggccg atttattact ttatacaagc
    10801 accaatgcaa aaaatgatga ttcatggctt tgaagttgac tgccggatta aagattttca
    10861 aagtcgatta aaatcaggag aaaatatgaa atatactaaa tgatattggg tgatatggat
    10921 gcaaatacta ctagtagaag atgacaatac tttgtttcaa gaattgaaaa aagaattaga
    10981 acaatgggat tttaatgttg ctggtattga agatttcggc aaagtaatgg atacatttga
    11041 aagttttaat cctgaaattg ttatattgga tgttcaatta cctaaatatg atgggtttta
    11101 ttggtgcaga aaaatgagag aagtttccaa cgtaccaata ttatttttat catctcgtga
    11161 taatccaatg gatcaagtga tgagtatgga acttggcgca gatgattata tgcaaaaacc
    11221 tttctatacc aatgtattaa ttgctaaatt acaagcgatt tatcgtcgtg tctatgagtt
    11281 tacagctgaa gaaaaacgta cattgacttg gcaagatgct gtcgttgatc tatcaaaaga
    11341 tagtatacaa aaaggtgacg atacgatttt tctatccaaa acagaaatga ttatattaga
    11401 aattcttatt accaaaaaaa atcaaatcgt ttcgagagat acaattatca ctgcattatg
    11461 ggatgatgaa gcatttgtta gtgataatac gttaacagta aatgtgaatc gtttacgaaa
    11521 aaaattatct gaaattagta tggatagtgc aatcgaaaca aaagtaggaa aaggatatat
    11581 ggctcatgaa taatttgaaa tgggtagctt attttttgaa atctcgcatg aactggatat
    11641 tttggatatt gtttttaaac ttgcttatgt taggcattag tctaatcgat tatgattttc
    11701 caatagacag tttattttat attgtttctt tgaatttaag tttaacaatg atttttctta
    11761 tattgacata ttttaaagaa gtaaaattat ataagcattt tgacaaagat aaagaaatag
    11821 aagaaattaa acataaagat ttagcggaaa cgccatttca acgtcataca gttgattatt
    11881 tatatcgtca aatctcagcg cacaaagaaa aggttgttga gcaacagtta caattgaaca
    11941 tgcatgaaca aaccattaca gaatttgtgc acgacataaa aacacctgtg acagccatga
    12001 aattattaat tgatcaagaa aaaaatcaag aaagaaaaca ggcattacta tatgaatggt
    12061 ctcgtataaa ctcgatgctg gatacacagc tgtatattac tagattagaa tctcaacgca
    12121 aagatatgta ttttgattac gtgtcactta aacgcatggt cattgacgaa atacaattaa
    12181 caagacatat tagtcaggtt aaaggtattg gttttgatgt tgactttaaa gtggatgatt
    12241 atgtttatac agatataaaa tggtgtcgta tgattattag acaaattttg tcaaacgcat
    12301 tgaaatatag tgagaatttt aatattgaaa ttgggacaga attaaatgat caacatgttt
    12361 cgttatatat taaagactat ggcagaggta ttagtaaaaa agatatgccg cgaatatttg
    12421 aacgaggatt tacgtcaacg gctaacagaa atgaaacgac gtcttcaggt atgggtctat
    12481 atttagtaaa tagtgtaaag gatcaattag gtattcacct gcaagtcacg tcgactgttg
    12541 gtaaggggac aactgtcaga ttgattttcc cattacaaaa tgaaattgtt gaacgcatgt
    12601 cggaagtgac aaatttgtca ttttaaacat gcgttttgtt acttagaatt gatacatcaa
    12661 tgcagcttca acgttataat aagatagatg ttagtcatat gttaaattga agatacaagt
    12721 gccaaagcct aaaggaaatg aagttaagat aaattatagg agtgttaaag tggcaatttt
    12781 agaagtaaaa caattaacaa aaatatatgg aactaaaaaa atggcacaag aagtgttgcg
    12841 agatatcaat atgtctattg aagaaggcga gtttattgct attatgggtc cctctggatc
    12901 tgggaaaacg acattattaa atgttttaag ttcaattgat tatatttcac aaggttctat
    12961 tacattaaaa ggaaaaaaat tagaaaagct ttcaaacaag gaattatctg atatacgcaa
    13021 gcatgatatt ggttttattt ttcaagagta taatttactg catacattga ctgttaaaga
    13081 aaacataatg ttaccactaa cggttcagaa gttagataaa gaacatatgt taaatcgtta
    13141 tgaaaaagta gcagaagcat taaatatatt ggatattagt gataaatacc cttctgaatt
    13201 gtctggtgga caaagacaac gaacatctgc tgcaagagcg tttattacat taccttctat
    13261 tatatttgct gacgaaccaa caggtgcact ggattctaaa agtactcaag atttattaaa
    13321 acgattaaca agaatgaatg aagcatttaa gtctacaatt attatggtaa cgcatgatcc
    13381 tgttgcagca agttatgcca atcgagtagt gatgctaaaa gatggtcaaa ttttcactga
    13441 attataccaa ggggatgacg ataaacatac ctttttcaaa gaaataatac gtgtacaaag
    13501 tgttttaggt ggcgttaatt atgaccttta acgagataat atttaaaaat ttccgtcaaa
    13561 atttatcaca ttatgccatc tatctttttt cattaattac gagtgtagta ttgtatttta
    13621 gctttgtagc attaaaatac gcgcataaac taaacatgac agagtcatat ccaattatta
    13681 aggaaggctc acaagtcgga agctactttc tatttttcat cataattgca tttttgttat
    13741 atgccaatgt gttatttatt aaacgacgaa gttatgagct tgcattatat caaacattag
    13801 gtttatctaa attcaacatt atttatatac taatgctcga acaattacta atatttataa
    13861 ttacggcaat attaggtatt attattggta tttttggttc aaaactgtta ttaatgattg
    13921 tctttacatt attaggaatt aaagaaaagg ttccaattat ttttagtttg agggcggtat
    13981 ttgaaacatt aatgttaatc ggtgtcgctt attttttaac ctctgctcaa aattttatat
    14041 tagtgttcaa acaatctatt tcacagatgt caaagaataa ccaggttaaa gaaacaaatc
    14101 ataataaaat tacatttgaa gaggttgttt taggcatctt aggtatagta ttgattacca
    14161 caggatacta tctatctttg aacattgttc aatattatga ttctatcggt atacttatgt
    14221 ttattttatt gtcaactgtg attggggcat acttattttt taaaagctct gtttctctag
    14281 tttttaaaat ggtgaagaag tttagaaaag gtgttataag tgtaaatgat gtcatgttct
    14341 catcatctat tatgtatcgt attaagaaaa atgctttttc acttacggtc atggcaatca
    14401 tttcagcgat tactgtttca gttctttgct ttgctgctat aagtagagcg tccttatcaa
    14461 gtgaaataaa atatactgca ccacacgacg ttacaattaa agaccaacaa aaagctaatc
    14521 aattagcaag tgaattaaac aatcaaaaaa ttcctcattt ttataattat aaagaagtaa
    14581 ttcatacgaa attgtataaa gataatttat ttgatgtaaa agcgaaagaa ccatacaatg
    14641 taacaattac tagtgataaa tatatcccta atactgattt gaaacgtgga caagctgatt
    14701 tgtttgtagc ggaaggttct atcaaagatt tagtgaaaca taagaagcat ggtaaagcag
    14761 ttataggaac gaaaaaacat catgttaata ttaagttgcg gaaagatatt aataaaatct
    14821 attttatgac agatgttgat ttaggtggac caacgtttgt cttaaatgac aaagactatc
    14881 aagaaataag aaagtataca aaagcaaagc atatcgtctc tcaatttgga ttcgatttga
    14941 aacataaaaa agatgcttta gcattagaaa aagtgaaaaa taaagttgat aaatctatta
    15001 aaacaagaag tgaagcgata agctcaatat caagtttaac cggaatatta ttatttgtaa
    15061 catcattttt aggtattaca ttcttgattg ctgtatgttg cattatatac ataaagcaaa
    15121 tagatgaaac cgaagatgag ttagagaatt atagtatttt gagaaagctt ggatttacac
    15181 aaaaagatat ggcaagggga ctaaagttta aaattatgtt taattttggg ttacctttag
    15241 ttattgcact atcacatgca tattttacat cattagcata tatgaaatta atgggtacaa
    15301 cgaatcaaat accggttttc atagtaatgg gattatacat ttgtatgtat gctgtttttg
    15361 cagtgacggc ttataatcat tccaagcgaa caattagaca ttccatataa aatatacaga
    15421 tggctttcag tagagtagtg gattcggatt cacgaactat actggaagct ttttattata
    15481 aatgaagaga agttatattt ttagcatgta tagttgaata ctgggttaaa ataccatatt
    15541 aataatgaag taaaggtatg agtgattatg aaagtgtttt gaatgaaata tatttaattg
    15601 gtgatgcttt taattgaaaa gattaacagg attcaacttt gtaaattgta ttaaatgtga
    15661 gaaaataaaa gtatattcat tgagagatat atgagtcaat gatcgtttta aacaagataa
    15721 gtgtatttta atatgtaaaa gttatgtaat aaatattgta tcgttgcaaa tttcccaact
    15781 atatccattt acaattttta gagttgtata gataaaaaat tgcctataca ataacttttt
    15841 caaaacaaat gcctactact atttaaattt tatgtaaaag ttaagtttcg gaattgttaa
    15901 aaatttgtaa attgaattgt gggattgtaa attttgtgct attttcatat aagggcaaat
    15961 acagtaaatt gtttactgtg aggcattttt gaaattaata tcagtacact aaaattatac
    16021 tgacgattga taataacaaa tttgtatcat tagtttgtaa attcacttgt cttaatttga
    16081 aacaaattat aactgaatgt gattggtgac aatcgcttaa atggaggatt ttaaatgttt
    16141 agtaagaaaa aagataagtt tatggttcaa ttagaagaga tggttttcaa tctggatcgt
    16201 gctgctattg aattcggtaa aatggatttc aatacacatt tagatttaaa agcatactca
    16261 gacaacatta aaacttatga gtcacatggt gacgaattag tacatcaagt aattactgat
    16321 ttaaatcaaa catttatcac accaattgaa cgtgaagata ttttatcatt atgtgatgca
    16381 attgatgatg ttttagatgc aattgaagaa acggcagcta tgtttgaaat gtattcaatc
    16441 gaatacacag atgaatatat ggctgagttt gttgataaca ttcaaaaagc agttgcagaa
    16501 atgaaacttg ctgtcggctt attagtcgat aaaaaattat cacatatgcg tattcattca
    16561 attaatatta aagaatttga aacaaactgt gatggtattt taagacagtc aattaaacat
    16621 attttcaata gcgaaacaga tccaatcact ttaattaaaa taaaagatat ttatgaaagc
    16681 atggaagaaa tcgctgataa atgtcaaatc gtagcaaata attttgaaac tattattatg
    16741 aaaaatagct aaggggagta tatatttatg tcatatataa tcatcgtcac tatagctgta
    16801 gttattttct cgctgatatt tgactttatc aatggattcc atgatacagc caatgcagta
    16861 gctactgctg tatctactag agcgttaacg cctaaaacgg caattttaat ggcagcagtg
    16921 atgaacttta taggtgcttt aacatttacg ggcgttgcag gcaccattac taaagacatt
    16981 gtcgatccat ttaaattgga aaatggatta gttgttgtgt tagctgcaat acttgcggct
    17041 attatttgga atttagctac ttggttttac ggaattccaa gttcgtcttc acatgcactt
    17101 ataggttcaa ttgcgggtgc agcaatcgca tctgaaggct catttggagt gttacattac
    17161 caaggtttca caaaaattat tattgtatta atcgtttcac cgataatcgc attttgtgtt
    17221 ggtttcttga tgtattcaat ttttaaagtt atctttaaaa atgcaaattt aacaagagcg
    17281 aatcgtaact ttagattttt ccaaattttc acagcagcgt tacaatcatt ctctcacggt
    17341 acgaatgatg cgcaaaagtc aatgggtatt attacgttgg cattaattgt tgctaatgta
    17401 cagaatgatg gcagcgttga accacagtta tgggtaaaat ttgcctgtgc gacagcaatg
    17461 gggcttggta ctgcaattgg tggctggaaa attatcaaaa ctgtaggtgg taatattatg
    17521 aaaatacgtc cagcaaatgg tgctgcggcc gatttatcat ctgcattaac aatttttgtt
    17581 gcatcatcgc tacatttccc attatcaaca actcacgttg tgtcatcatc aatcttaggt
    17641 gttggtgctt ctaaccgagc taaaggtgta aaatggagca ctgcgcaacg aatgatcatt
    17701 acatgggtga ttacattacc tatttcagca ttgttagcag gtttactatt ctatatactt
    17761 aacttatttt tctaattgaa aataaaacta aactgaactt cagtatcaca aacatatggt
    17821 ggtattgaag ttcagttttt tatgtaagta gataacatat tttatagagt ttacgtagaa
    17881 tgaccaagac aaagcatttt aattaggggt ctaagttata aatttattcc atggaacata
    17941 aacagttagg tcattaaaat ataagtaagc gtaaaataat gatttaatag tttatattct
    18001 tcaatgaagg aataattaaa t
//
LOCUS       DBNENW010000044        17652 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNENW010000044 DBNENW010000000
VERSION     DBNENW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.044"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..267)
                     /locus_tag="AC1LW8_001174"
     CDS             complement(<1..267)
                     /locus_tag="AC1LW8_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIP4773021.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNYLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALDLDNLMSVCYSCHNKIHAN"
     gene            complement(424..861)
                     /locus_tag="AC1LW8_001175"
     CDS             complement(424..861)
                     /locus_tag="AC1LW8_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP4773022.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            complement(874..2240)
                     /locus_tag="AC1LW8_001176"
                     /pseudo
     CDS             complement(874..2240)
                     /locus_tag="AC1LW8_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
     gene            complement(2221..2511)
                     /locus_tag="AC1LW8_001177"
     CDS             complement(2221..2511)
                     /locus_tag="AC1LW8_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HIP4773023.1"
                     /translation="MKESTLEKYLVKEITKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHTVYVLWNKEQVDEFIRMVGGTFGD"
     gene            2645..2749
                     /locus_tag="AC1LW8_001178"
     CDS             2645..2749
                     /locus_tag="AC1LW8_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773024.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHHQK"
     gene            complement(2852..5299)
                     /locus_tag="AC1LW8_001179"
     CDS             complement(2852..5299)
                     /locus_tag="AC1LW8_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HIP4773025.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTHWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVAGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDTILNEYVDWTDTLEWPTSS
                     REESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAIETFIPDLYEKHSNNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDTKTDIPVNRLPSY
                     KAMQQRAQNDEIVKKQLINDKMTDAMQDFDEIENSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWEDLTIFERRRFYQGDVDMLPTGNVDY
                     IERDKVCALEVFVECFGKDKGDSRGSMEIRKISNVLRQLDNWSVYEGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(5351..5551)
                     /locus_tag="AC1LW8_001180"
     CDS             complement(5351..5551)
                     /locus_tag="AC1LW8_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HIP4773026.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNAYLFKQLVKN
                     NGVEGIEDYENEVERIRKRFKS"
     gene            complement(5619..5771)
                     /gene="rinB"
                     /locus_tag="AC1LW8_001181"
     CDS             complement(5619..5771)
                     /gene="rinB"
                     /locus_tag="AC1LW8_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HIP4773027.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFEKI
                     RAEVSW"
     gene            complement(5768..5974)
                     /locus_tag="AC1LW8_001182"
     CDS             complement(5768..5974)
                     /locus_tag="AC1LW8_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HIP4773028.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFIVVEAESKEEAKEKY
                     EAQVKRDAVIKLGQLFENIRECGK"
     gene            complement(6011..6547)
                     /locus_tag="AC1LW8_001183"
     CDS             complement(6011..6547)
                     /locus_tag="AC1LW8_001183"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIP4773029.1"
                     /translation="MNNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKDTEF
                     HKMFNFKDKEFAQGAVVSTPQIIFKEFYPDQLAIVIAIDIAYNLYTIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(6540..6710)
                     /locus_tag="AC1LW8_001184"
     CDS             complement(6540..6710)
                     /locus_tag="AC1LW8_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773030.1"
                     /translation="MKKFNVQITYTGMIEEAIEAESLEEAEFEAHDIARMEVPFDCDE
                     FEINVEVEQENE"
     gene            complement(6697..6951)
                     /locus_tag="AC1LW8_001185"
     CDS             complement(6697..6951)
                     /locus_tag="AC1LW8_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HIP4773031.1"
                     /translation="MNNREQIEQSVISTSAYNGNDTEGLLKEIEDVYKKAQAFDEVLE
                     GMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQENEKI"
     gene            complement(7143..7466)
                     /locus_tag="AC1LW8_001186"
     CDS             complement(7143..7466)
                     /locus_tag="AC1LW8_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773032.1"
                     /translation="MNCFKFSFINENKELILDSESNIFFSLKDCANKLDLVVKFIHWV
                     SRCCIENISPERTQVFLQTGFELYIGKHLTNNDYEYMYRCFGNGLNSDGAYSYARRLL
                     NIPEG"
     gene            complement(7496..7645)
                     /locus_tag="AC1LW8_001187"
     CDS             complement(7496..7645)
                     /locus_tag="AC1LW8_001187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773033.1"
                     /translation="MNYETGFQLGVMEARLKKMRKQRDACKKQRDELIVDIAKLREKQ
                     KHLMR"
     gene            complement(7660..7908)
                     /locus_tag="AC1LW8_001188"
     CDS             complement(7660..7908)
                     /locus_tag="AC1LW8_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HIP4773034.1"
                     /translation="MTMTDNVCKEYLNQFFGSKRYLYQDNEGVAHIHVVNGTYYFHGH
                     IVPGWQSVKKTFDTAEELEIYIKQHGLEYEEQKELTLF"
     gene            complement(7908..8162)
                     /locus_tag="AC1LW8_001189"
     CDS             complement(7908..8162)
                     /locus_tag="AC1LW8_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HIP4773035.1"
                     /translation="MSVISNRKVDMNEAQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWE"
     gene            complement(8162..8562)
                     /locus_tag="AC1LW8_001190"
                     /pseudo
     CDS             complement(8162..8562)
                     /locus_tag="AC1LW8_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SA1788 family PVL leukocidin-associated protein"
     gene            complement(8562..8747)
                     /locus_tag="AC1LW8_001191"
     CDS             complement(8562..8747)
                     /locus_tag="AC1LW8_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HIP4773036.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(8760..10712)
                     /locus_tag="AC1LW8_001192"
     CDS             complement(8760..10712)
                     /locus_tag="AC1LW8_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIP4773037.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGPISAI
                     DMTKVDNEPFHADYETFKIALFDPTVKKYAFNANFERTCLAKHFNKPMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMAIANKIKDFPVTAIEQTYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLKQAKQLTGLENPNSPTQLLAWLKDEQGLDIPNLQKKTVQDYLKESTE
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGLRFYMKKGFLMIELPSGRALAYPKALVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(10781..11338)
                     /locus_tag="AC1LW8_001193"
     CDS             complement(10781..11338)
                     /locus_tag="AC1LW8_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HIP4773038.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQEAKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(11364..12530)
                     /locus_tag="AC1LW8_001194"
     CDS             complement(11364..12530)
                     /locus_tag="AC1LW8_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HIP4773039.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSSVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVSESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTVRMTIIQPRINNFSTEELPISRLLQWGTDFVKPLARL
                     AYNGEGEFKAGSHCRFCKIKHSCRTRAEYMQNVPQKSPHLLSDEEIAELLYKLPDIKK
                     WADEVEKYALEQAKENDKTYPGWKLVTGRSRRVITDTKAVRGRLVEAGYKPEDITETK
                     LLSITKLEKLIGKKAFSKITEGFIEKPQGKLTLAIESDKRPAIKQSAEDDFDKL"
     gene            complement(12527..12889)
                     /locus_tag="AC1LW8_001195"
     CDS             complement(12527..12889)
                     /locus_tag="AC1LW8_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773040.1"
                     /translation="MAILEDIFEELKLLNKNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLETIEEVKETSTDLTKDYILSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMEARINA"
     gene            complement(12904..13227)
                     /locus_tag="AC1LW8_001196"
     CDS             complement(12904..13227)
                     /locus_tag="AC1LW8_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HIP4773041.1"
                     /translation="MTLQQKILSHFATYDNFNPDDVVEVFGVSKTHAKSTLSRLKKKG
                     KVEMESWGKWRVIEAQLHLTVVERKKEILEEQFELLARLNEQSDDPREIEDRIKLMIR
                     LANQF"
     gene            complement(13306..13467)
                     /locus_tag="AC1LW8_001197"
     CDS             complement(13306..13467)
                     /locus_tag="AC1LW8_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HIP4773042.1"
                     /translation="MSDTYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYGE"
     gene            complement(13480..13743)
                     /locus_tag="AC1LW8_001198"
     CDS             complement(13480..13743)
                     /locus_tag="AC1LW8_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773043.1"
                     /translation="MPKIIIPPTPENTYRGEEKFVKKLYAKPTEIHQLFGVSRSTVYN
                     WLKYYHEDDLGIKNLCIIYSPAGQLINIPKLEAYLIKRQEKIL"
     gene            complement(13768..13983)
                     /locus_tag="AC1LW8_001199"
     CDS             complement(13768..13983)
                     /locus_tag="AC1LW8_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773044.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            14038..14403
                     /locus_tag="AC1LW8_001200"
     CDS             14038..14403
                     /locus_tag="AC1LW8_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773045.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            complement(14372..14617)
                     /locus_tag="AC1LW8_001201"
     CDS             complement(14372..14617)
                     /locus_tag="AC1LW8_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIP4773046.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            14673..14882
                     /locus_tag="AC1LW8_001202"
     CDS             14673..14882
                     /locus_tag="AC1LW8_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773047.1"
                     /translation="MKACLYLSNDKFVEIDNLEKVIKSGHRGTVEISKEKIKSSLFTN
                     GSYTFVGDKIVAIASAKIEFIEFID"
     gene            complement(14872..15015)
                     /locus_tag="AC1LW8_001203"
     CDS             complement(14872..15015)
                     /locus_tag="AC1LW8_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773048.1"
                     /translation="MLQKFRIAKEKSKLKLNLLKHANSNLETRNNPELLRAVAELLKE
                     INR"
     gene            complement(15044..15820)
                     /locus_tag="AC1LW8_001204"
     CDS             complement(15044..15820)
                     /locus_tag="AC1LW8_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HIP4773049.1"
                     /translation="MQALQIVEQNETHYVDSREVAEMIGKRHDNLVRDIKGYIKVLED
                     SSKLSSHNFFEESTYVNSQNKVQPCYLLTKKGCDIVANKMTGSKGILFTATYVDAFHK
                     MDEYIKQQAQLNVPQTPMQALEMMFKAQKDQEQFNKQMQQEITGIRHIVGIETKNWRN
                     DTNKMLSAIAQHLGGGAMHQKVKSEAYKALEEKGRCNLKIRMQNRKGKMLANGATKTQ
                     INKLSKLDVITDEPRLVEIYISVIKSMAIKYGVDISQFEI"
     gene            complement(15834..16070)
                     /locus_tag="AC1LW8_001205"
     CDS             complement(15834..16070)
                     /locus_tag="AC1LW8_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773050.1"
                     /translation="MPIDAKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFFNEDLRKKKVLT"
     gene            16222..16536
                     /locus_tag="AC1LW8_001206"
     CDS             16222..16536
                     /locus_tag="AC1LW8_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773051.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSK
                     RK"
     gene            16549..17010
                     /locus_tag="AC1LW8_001207"
     CDS             16549..17010
                     /locus_tag="AC1LW8_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HIP4773052.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVR
                     NLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            17028..17612
                     /locus_tag="AC1LW8_001208"
     CDS             17028..17612
                     /locus_tag="AC1LW8_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001636973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773053.1"
                     /translation="MKRLLGLTLASALVLGACGSHDGDKKEESKKTETKKDNKDKKKE
                     TKEKAEAKKENANQNDNNNQVNNENNTNVNNDQQTNRPLTKDEISQRVKNGHNVNGMV
                     DADGNTWYQAQGAGDVIGYTKPDGTQCTVGGCVTPQQQEQINEANYKEMEKYGYSREK
                     YDAIQKEASKLQQQKENGEITAEEFTNRYIELYD"
BASE COUNT         5138 a         3450 c         2530 g         6534 t
ORIGIN      
        1 atttgcatga attttgttat gacagctata acaaactgac attagattat ctaagtctaa
       61 agctttgtta aaatcttcat caacataaat aatgtgatgt acaatgtttg catctgttac
      121 aatatcttcg cgtaaacaca tttgacaaag ataattatct ctatctaatg ctatctctct
      181 taacttcttc catgcttttg aatgatagaa ccaatcgtat tgatatgact tacgaccatg
      241 cttataaatg ttattatgct tggtcatctc ttacacctct ttgattgcat aacaaaagac
      301 acaccgcata gcgatgtgcc tcgtttactt atgtcgtata acttttagat aactttatac
      361 atctttccga tactatcata ttactacaga tttgtaggcc ttttgcacaa tctttgcaca
      421 atgttatttg atacctgcat gatacgctac cgctttaaca aagttctttc gtatagtagt
      481 aacagtatta cgatgcatat ggcattcatg tcctatctgt tccatcttta acttcttttc
      541 tttattccaa tacttgagcc ttattacttt cttatgatct tcaggcaact ttaagtattc
      601 actctcaact gcttcgacca tttcttcaag gtttcgtaac atcttattag ttaataatct
      661 agttgccatt agttcagttg ttctaactgg ttcgcctttt tgtaatggtc catatacaat
      721 attggaatct tgttccttcg ttggattaag tatttccaac ctcaatcttt ttatttcttt
      781 cttgttctca tttaaattat atatttctga ttcaatatat ttaaatgttc ctggcttgat
      841 atcatatatt gtgttcccca tgttagacct ccattactta tgcttagcta ttcttgcttt
      901 aatagctttc attaattctt cttgcgttag ttctttattt tgtaaagctt tatatactct
      961 ttgatctatt gtgttatcgg tcatgatgtg atgaataata gtcgtatgat tttgtccttg
     1021 tctatataat ctagcatttg cttgttggta taattccaag gaccatgtaa gtccaaacca
     1081 aacaataatg tgcccacctt gttgtaagtt taatccatgt cctgcacttg ctggatgtgc
     1141 tataagcagc ttaatgtctc cactgttcca acgttcttta tagtttgaat cctctaatgt
     1201 ggttgcttcc ttaaaccttt gaagtattct ttctttatcg tgtttgaagt tataaaacaa
     1261 tagtattggt tggccttgag actcctctat aatttcctct aacttatcta acttcttatc
     1321 atgtataagt cttacatctt cctcatctgt ataaactgcg ccgttagata gttgaagtag
     1381 tttctgactt aatgatgccc cattttgagc tacaactgtt ccttcttctt ccgattctaa
     1441 aatatagttt ttttctaatt cttcatatac ttttctttct ttttctgata agactactgt
     1501 ttgtttagta tcaactctgt caggcatatc cagataatct ttcgctttca tgcttaaaca
     1561 tatatcttct atttgtttat atatcttttc ttcagatccg tctcttagct cccacttaaa
     1621 aatatgttcg ctaacttgat gagttggttt aaagtacctt tctcgataac gactgaatga
     1681 agactcaagt ctttcacctc tgtctatcaa ataaacttga gcccataaat cctgtaaact
     1741 atttggacta ggtgttcctg ttaatcctat aaatctatta atgagtggta atttcttttt
     1801 aatagattta aacctttgac tcttaggact tttaaatgta gacagttcat caatcacaac
     1861 catgtcaaat ggccattttt tttatattga tcacataacc atttagtatt ttctttattg
     1921 gttacataga tatcagcctc tgtgtttaat gcatcatttc tttctttagg tgttcctaaa
     1981 actaaagaca ctttcagatg atttaaatgg ttccacttat caacttcatc aacccatgta
     2041 tctttagcaa cttgtttagg tgctataact aacatttttt tagtgtctaa caactgcaat
     2101 tcactaaatg ctgtaagtgt cgatactgtt tttcctaaac ccatatcaag gaataaaccg
     2161 aatttctcat tatcaatcac tttatctatt gcatactttt gatagttatg tggtttgaag
     2221 tcaatcgcca aatgttccac ctaccattct gataaattca tcaacttgct ctttattcca
     2281 taacacatat actgtatgat ctctattttc aaattgccga tgcacatatt tttgtaaagg
     2341 atgcaactta cctttttctt gcttcatttc tacaaaatat gtttttcctt ctggcatgat
     2401 aataattcta tccggcacac ctcttgttcc aggtgcaacc cattttaaac atagaccgtt
     2461 taactttgtt atctctttca ctaaatattt ttctaatgtc gattctttca tttattcacc
     2521 ttgtatacaa aatttatatt tgtgttccga tgttgcatca attcttgcca aacttttaaa
     2581 aatagctgtt agagggttac ccctataccc ctttactccc taacactact ttttaaactt
     2641 tatagtgaat ttgatgcaac attggaaaca aacagggttg aaccttacag cgagaaggga
     2701 aagaggtgtt gtatcatttg ttgcatcaat gttgcatcac caaaaatgat acaacaccta
     2761 cgattacttt ttacaccacc gtgttgcatc actcaaaaaa tgatgcaaca tttgatacaa
     2821 catctaagaa tgtatattta ttcaatattc cttatattaa atcctctaaa ctttcatctc
     2881 ttacatacgc tatctgtaca ccataatctt ttccaaatcg aattttccca cttttattgc
     2941 cttcatatac agaccaattg tctaattgtc ttaagacgtt agaaatcttt ctaatttcca
     3001 tagatcctct actatctccc ttatctttac caaaacattc aacaaacact tcaagcgcac
     3061 agaccttatc tctttcaatg taatctacat ttcctgttgg taacatatca acatcacctt
     3121 gataaaatcg tcttctttca aagatagtta agtcttccca attgcttggg attggcgtgt
     3181 taagatattc atcaataata cctgtatatg gagattcttc agtatgttta ctttggattg
     3241 aacgcatttc ttcttctagt tcagggttaa ggaacaactc ttctccttgt tcataatagt
     3301 atttagcttc tgcccagatt tggtcgatct cttctttggt tagtttagac cagttcactt
     3361 caactctctc tggatttaca gtcattggcc aaaaacgtct tccaccagtt tcatctctta
     3421 agaaatcaac tttattagtt gtaccaatga aaatacattg ccttggaaaa tcttcaatat
     3481 aatgtccata agcaacacga aaccggtcaa cttgtttaga tataaaatgc ttaatagctt
     3541 caacttcagc ttttcttgta gctgcaagtt ctgccatttc cattagccaa acgccttgta
     3601 aggcctcata agcttcttta cctgtaacag aaactaaact gtcagaaaac catgcaccac
     3661 ctaatttttt tagcaaagca gatttaccta caccttgagg accataaagt gtaagcatat
     3721 agtcaaattt acatccaggc tccattactc gagcgattcc agcagtcaat gcttttttgg
     3781 tagttgttct attcacttca gtatcttcaa cacctaagta tttgataaat aacttttcaa
     3841 gacgtttatg tccatcccac gatattttat ttagataatc ccttactgga tgataagcat
     3901 tttgcattgc tacgcttata atggcatctt ttgttttacc tgaatggtgt atgtcataaa
     3961 tcttttcgat ataacttctt aaactgctat catcaccgtc ttgccattga cgtgtcttaa
     4021 aattagtatt ccatggcact ttccctaaac attcaatttg tttcgtaaat tcgttaaagg
     4081 ctatttttcc ttttaaattt ggatcattac gcaatataat ttctatattt ggaatactag
     4141 ctttgaaagt acctttcgaa gtaatttcta acgtctcaga ccatgcatca tcgctatttt
     4201 ctatttcatc gaaatcctgc attgcatcag tcattttgtc attaattaat tgctttttaa
     4261 caatctcatc attttgcgct ctttgctgca ttgctttata actaggtagt cgattaactg
     4321 gtatatctgt tttagtgtct tcatcttgag caccatataa gtgtatgcgt actaaatcaa
     4381 aactgttcac aagcataccg cttacgggat ccgtattatg atgagaatag gcaaacttgt
     4441 tattttcata taacaccaat ccacctgcag ttgaaccttc atggtaggta taacggttat
     4501 tagaatgttt ttcgtataag tcaggtataa aagtttctat agcttcttct atcgtataag
     4561 ctctgcaaaa tgcgccaaca attcccggct tttcttctgg gtcaccttgc ttatctgcta
     4621 atcttttagt cttactctct tcccttgaag acgttggcca ttctaatgtg tcagtccaat
     4681 caacatattc atttaaaatt gtatctggat ctaacaaagg taaatcttca taggtaaaga
     4741 aaaattctgc atcattgcta gttgaaggcc aatacattaa cctatgtggt tgataagttg
     4801 tatcatcgaa gtaatccatg ccaacgatat ctgcgacttt acgcccaata gcttcatact
     4861 catctgcatt tacatttcgt tttaaaggaa tcactaaacg cagtcttgga cttatctctc
     4921 tatgcttatg tgttgaatat aaacaatatg caaaatcata aaacatagat aatatgtcag
     4981 tcatatcttg agcagcataa tcgatatcaa gtgttagcat tgaacgattc atgacttgac
     5041 cagcacgccg tttgccttct tttaaatatc cagcgacaaa tccgccaaca tcttttatat
     5101 cggcttgttc agacttagac attttattgt actcagttaa atcttcttta gttctaactg
     5161 tttgtgctag cttctgcata aagtcagacc aagccatatt gtgattagtc caatgtgtag
     5221 ataaacgact agcagcataa gaatatgaga catcacgatc atatttaatt gtttctattt
     5281 gagtgacttt gtctaacatg ttcggctcct ttcattattt tagatagagc agagaagcca
     5341 acgcctctct ttagcttttg aatctttttc taattcgttc aacttcattt tcgtaatctt
     5401 ctataccttc gacaccatta ttttttacta actgcttgaa aagataagca ttcatatact
     5461 ccaatgcttc tatggttttc atcttatgag aaatgctact taacaagatc aataaaaata
     5521 tagctaaaac aattgaaatg acaatccaca tagttacaac acctccagtg ctattgctaa
     5581 acacattaat ataattaatt caaaaatgat aatagctatt accatgaaac ttcagctctg
     5641 attttttcaa agtcactcgg cgcctctaca tcatcgttag ccgtcatcat aatatatact
     5701 tgctcagtta catacttacc tagctcatac atcgctagta agaataatag tcttagtatt
     5761 tgtttaatca tttcccacac tcccttatat tttcaaacaa ctgacctaat ttaataactg
     5821 catctctttt aacttgcgcc tcgtacttct ctttcgcttc ttctttactc tctgcctcaa
     5881 caactataaa cctttgattg ctcttagctc gagttatgtg tgtatgcttg cgtcctgttg
     5941 aatctttgaa tgtcgtgact aagtattgtg tcacttcccc aaaacctcct tgactcgata
     6001 taagatgtct ttacacgtat ccttttcctg cgtctgctgt tccatcttgt ctttcgtggt
     6061 tccttttcat tttctttttg tatgcgtcaa tgagttggtc gatagtatat aagttgtaag
     6121 ctatgtctat cgctatcaca attgccaatt ggtcgggata aaattctttg aatattatct
     6181 gtggtgtact aacaactgcg ccttgagcaa attctttatc tttaaaatta aacattttgt
     6241 gaaattctgt atctttaaaa cttgattcaa tcgcttcttt tatctcttct gatgacactc
     6301 ctacttgatt cgcaatactc aaaccaaacg ccaacatatc cgccaactca tctaactgaa
     6361 catctaacgg cttacctggt ttcttcttcc agttcttaaa cgtttccaat gtattaaacc
     6421 attcaaagaa ttcaactaca tatgcaattt tgctatctcg taagttcagc gttggtattc
     6481 tatcgtcgaa ctctttttgt atttgtaata actcttgtaa ttgatcaatt gttaatgtgt
     6541 tattcatttt cctgttccac ctctacatta atttcaaatt catcacaatc aaatggcact
     6601 tccattctcg caatatcatg agcctcaaat tctgcttctt ctaaactttc agcctcgata
     6661 gcctcttcaa tcatgcctgt atatgtgatt tgaacattaa attttttcat tttcctgctc
     6721 ctcctcatat ttatagacca cttgacctgc cataatccct actgcttcat caagttcaac
     6781 accttcttta actgaatgtt gaatagcatt tgtcattccc tcaagtactt catcaaacgc
     6841 ttgcgctttc ttataaacgt cctcaatctc ttttagcaat ccctctgtgt cattgccgtt
     6901 atacgcacta gtacttataa cagactgttc gatttgttcg cgattattca ttagtgtctt
     6961 cctccatgaa aattttattg tttaattcca ttccaaattt aactctttca tcatcgttgc
     7021 cgaatttgtt tattaaatct ttttcaacac tcttgcaata cctatcccat gcgcttgctt
     7081 tcttctccgg ctctttgata cgttttttaa atttttcaac acttttttat ctactgtcat
     7141 ttttaaccct ccggaatatt taataatctt ctggcataac tatatgcgcc atcactattt
     7201 aatccgttgc caaagcatct atacatgtat tcgtaatcat tatttgttaa atgtttgcca
     7261 atataaagtt cgaaacctgt ttgtaaaaaa acctgtgttc tttcaggaga tatattttca
     7321 atacaacatc tgctaaccca atgaataaat ttaacaacta aatctaattt gttagcgcaa
     7381 tcttttagtg aaaagaaaat atttgattcg ctatcgagga taagctcttt attttcattg
     7441 ataaaactga atttaaagca attcatcatt tcgaatactt cataaatcag attatctatc
     7501 tcatcaaatg cttttgcttt tctcttaact tagctatatc cacgataagc tcatcacgtt
     7561 gcttcttgca cgcatcacgt tgttttctca tcttcttcaa cctagcttcc attacaccta
     7621 gttggaaccc tgtttcatag ttcactttca taacctcctc taaaataaag ttagttcctt
     7681 ctgttcttcg tattccaaac catgttgctt tatatatatt tcgagctctt cagcagtatc
     7741 aaatgtcttt ttaacacttt gccaacctgg cacgatatgc ccatgaaagt aataagtgcc
     7801 gtttactaca tggatatgtg ccactccttc gttatcctga tacagatatc tcttagaacc
     7861 gaaaaattgg tttaagtatt ctttacacac gttatctgtc atggtcatca ctcccacaag
     7921 tcaaaagctc tttggacgta aaacttcgcc tttgctaaat cctcatgacc attctttaac
     7981 ggtgctctag acaagtattt gattgcatta cctattgcga atgctagttg aggtggatac
     8041 tgtgcagtaa cctgttcgat aaaatctata atttcaatgt cgccgtatgt gtagtgcgct
     8101 ggttgcttaa cattgtcttg cgcttcgttc atatctactt ttctgttact gattacgctc
     8161 attatgcttc actccatttc ttgaacattt ggttataagt gacatcgaac cagtacggat
     8221 cacgtgaatg tttttgtggc gttccatcat aaagccatgg tctcaatctt ctctttcttt
     8281 cttcttcata ttccgctctc acatttcgtt ggtataggtt caaaatcgct tttttctgat
     8341 tttttctctc tctttttctt catcttttat ttgactctgc atatattcaa cttcatcttt
     8401 agattttgag tcttttcttc cacacaataa ttcatcgccg cgcattttat gtttgtatct
     8461 atatctaaga agttctggag atatgtgata tttttctgaa acttctctca atgtcattag
     8521 ttttcctttg atacgcactc ttataacttt tcttctagcc atcattccac ctctaaatct
     8581 aaaaccttaa tatttataac gttatatttt aatagttcac ctggattatt aaataaatag
     8641 tccgccaaat tttctttttc tttatcaatc tgattgtaat taacactttc gacttctgta
     8701 ggaattctaa tgtcaacaga agcattgata taagcttgat gttgcatgca atcacactcc
     8761 taatccttca tataaaacgg agaagtaaac ccgtcactat tcaaattcaa tccttttgcc
     8821 caatcaacag gcttattcat gatagtttcg atttccttaa gtccatttga acctctaggt
     8881 atttctacaa ttacttcatc atggacatgg ccaactattt taaaacctga tgcttcaagc
     8941 cttgctatag aaatcgcaag taaatccctt gcagttgctt gaacaatatt ctcgactaac
     9001 ttcccaccat acgtttttaa ctttgaccat ttacggttaa gatctaaccc cataaattca
     9061 acaacttgac taccccaact attttcacca actaaagctt ttggataagc taaagctctt
     9121 ccactaggca gttcaatcat taaaaaacct tttttcatat aaaatctaag tccatgtgta
     9181 tgatgcgtct ttcgggattt tacagtatta attgcagcct cttggcaagc cttccaaaaa
     9241 ttaactatgt taggatttgc gttacgccaa ctatcaacta aaccttgtaa ctcgttttct
     9301 tcaatgccca tttccaatgc acccattgct tttaaagctc cagcgccacc ttgatagcct
     9361 aaagctaatt cggacacttt tcccttttgt ctgagagggt cgcctttagt tatgctttct
     9421 accgggacat taaacatttg agaagccgat gcttcatata tctttccgtg tgtgttgaac
     9481 acatctaaac gccattgttc ttttgcatac catgctatga ctcttgcctc tattgcagaa
     9541 aaatcactta ctgctagttc attaccttct tcagcagtaa atgtcgtcct aactaattga
     9601 cttaataagt cttgaggatg aacattgagt aataaatcta aatcgtcaaa acgttgttct
     9661 ttaataagat ctcttgctat ttctaattca gtatctgaaa tataatgctt tgttaaattc
     9721 tgaagttgta cacctctacc tgcccatctt ccagtaccgg caccgtagaa ttgaaacaga
     9781 cctcttaccc gttcatcact gcacatcatg tcatgcattt tgttgtattt tttcacacta
     9841 gttttagaca tttgcaatct gatttctagc atttttttag ctttttctgt tgattctttt
     9901 aagtaatcct gaactgtttt cttttgtaaa ttaggtatat ctaacccttg ttcatccttt
     9961 aaccaagcca atagttgtgt aggactatta ggattttcta aacctgttaa ttgtttagct
    10021 tgtttaagca attcttcttt actctgttta tcgagcacat tagcacctaa catcaaggac
    10081 ttagaaagct taatacctct gtcgtttata tgttggtcaa aaacccaata tgtttgttca
    10141 attgcagtta ctggaaagtc tttaatttta ttagcaatcg ccatttctac ttctacatct
    10201 cgaatacagt aatctataaa ttgttgccat ttttcaagat catgttcagg taggtttctt
    10261 gttcttcctc cattaacttt tgttggttta caaggtatag aaaaataacg gattaaattt
    10321 ttacctgctt tatctttttg attttgtaac cttaaaactt ctccgacttt atcaagcgaa
    10381 gcaggtaatc caatacgcat tgaattaacc attgtgcaaa tccattcttc aggtggcatc
    10441 ggtttattaa aatgtttagc aagacatgtt ctttcaaaat tagcattgaa tgcatatttc
    10501 tttacagtag ggtcaaaaag agcaatttta aatgtctcat aatcagcatg gaaaggctca
    10561 ttatctactt tagtcatgtc aatcgcacta atcggtccac catctattga ataagctata
    10621 attaaaattt cgaaatcttc agcttctgtg tatttatagg caccacattt ggatatatcg
    10681 ttactgctat aggtttcaat atctatattc ataaatttca aattcttgac acctcaattt
    10741 ctttaaaatt aaagtggggc taaaaacccc acctattgac ttataagaaa tcctcatcat
    10801 cagtgtctaa ttcatcgaaa tcatcttctg ctgcactcgc accaccaaga ggttcgcctt
    10861 tttctacaag ttgaatgttg ttcaatccaa ctgcgatacc cttattacca tttgtgttga
    10921 aaggaaatag attaattgaa gctctaatat aatcaccact tacaacagtt ccagaatccg
    10981 ttaatctaat tttgttttgg tcaataatac caggtgcttg tttgcttgat gcgttaataa
    11041 aataagcgtc ttgataattg acatcatctt ctctttcagt atctccatca cgtaatggaa
    11101 gtttcagatt tgcaggaact ttgcctccaa acttactaac ttttccttct tctttagcag
    11161 cttctatagc ttgttcaatg gcttttatcg tacttgtatc tgatttagga atgattaaac
    11221 tgattgaata ctttgcttct tgcccttctt gcatactgtg aggttcaaaa atatgtgcat
    11281 atgatgctct tacttttcct gtaatcactt tagttttatt taatactttt gctttcatgt
    11341 ttatataccg tccttttaaa tttttatagt ttgtcaaaat catcttcagc agattgcttt
    11401 atagctggtc gtttatccga ctcgatagca agtgttaatt taccttgcgg cttttctata
    11461 aagccctctg taattttaga aaatgctttt ttaccaatta atttttctaa tttcgtaatg
    11521 ctaagtaact tggtttctgt aatatcttca ggtttataac ccgcttcaac taacctgcct
    11581 cggactgctt ttgtatcagt tatcactctc cttgaacgtc ccgtgactag cttccaacct
    11641 ggatacgttt tatcattctc tttcgcttgt tctaacgcat atttctctac ttcatcagcc
    11701 cattttttga tatcaggcag tttatataaa agttctgcaa tctcttcatc acttaacaaa
    11761 tgtggtgact tttgaggcac attttgcatg tattctgcac gtgttctaca tgaatgcttt
    11821 atcttacaga atctacaatg actacctgct ttaaactcac cttcaccgtt ataagcaagt
    11881 ctggctaatg gtttaacaaa atcggttccc cattgaagta atcttgatat gggtaactct
    11941 tcagtagaaa agttatttat tcgaggttgt atgatagtca tgcgaactgt atgaatgtca
    12001 tacattaaac taagcagttc atatgcgccc aatccatata atctaagttg aggattatct
    12061 atagctgaaa cttcaatgcc tttaccgtat ttaagatcaa taatttcaag cacaccacct
    12121 gaaaatataa tgacatcacc agtaccaaaa gattcagaga catatttacc taaatccaat
    12181 tttgtttcaa ataaagctat tacatcatta tccctactca aagcttcgtt atatttttct
    12241 tctacattag ctacatactc ttcaacatat tcacgcaact cttcactgta atattgattt
    12301 cgcttataat tttgaaaagc tttattaaac tcaaactgtg ttaggccttc atatttaaga
    12361 ctgaaatata actcacttaa ctcatgagcg aatgtacctt cttcagcaaa aactgaactt
    12421 ttatctgcaa taccttcact tgccttaata ctcggtggac agtttagcca ttgttttgct
    12481 ccacttgcac ttagctttgc atgagctcta tttgagtgat ctagcttcat gcattaattc
    12541 tcgcttccat gaaatcaaca attttttcat aatgttcttc tttgatagta gatagcttat
    12601 ccgcaccaag ttcgttaagt ttatttctaa attctttctt atcagaagta tctgcttttt
    12661 taaggaactc ttttcctact gataaaatat aatctttagt taaatcagta gacgtttcct
    12721 taacttcttc aattgtttcc agttgagctg tttcttcttt tggcattggt gcttctttaa
    12781 ctttctcttg tacgattgat gaatccacag ttgatagttc agtatttaac acacgtaaat
    12841 tcttatttaa tagttttaat tcttcaaaaa tatcttctaa tattgccatt gattaaatcc
    12901 tccttaaaat tggttagcta gacgaatcat taacttgata cgatcttcta tttctctagg
    12961 gtcatcactt tgttcattca atcttgctaa caattcaaat tgctcttcta aaatttcttt
    13021 tttacgttcg acgacagtta aatgtaattg tgcttcgata acacgccatt ttccccaact
    13081 ttccatttca acctttcctt ttttcttaag tctcgaaagt gtggattttg catgtgtttt
    13141 cgatactcca aaaacttcaa ctacatcatc aggattgaaa ttgtcatatg ttgcaaaatg
    13201 tgatagtatt ttttgttgta aggtcatatt aataactcct tatataatta tttaagacaa
    13261 ttgctcatct tgcactgtta cttgctccaa caagtagcag tttttttatt ctccataaaa
    13321 gtattcttta tagaatatga atgttgcgat acttgcgaat cccgcaatcg accacgctgt
    13381 agtgaagtat agaaacggca tgagtacaat cgctaagacc gtgaagcaca gtactgctac
    13441 taggtaactt ttatatgtgt cactcatttt attctctcct taaagtattt tctcttgcct
    13501 tttaattaaa tacgcttcta acttcggaat attaattaac tgtccagctg gagaatagat
    13561 gatacataag ttttttatac ctaaatcatc ttcgtgataa tatttcaacc agttgtatac
    13621 tgtacttcta cttactccga atagttgatg aatttctgtt ggttttgcgt ataacttttt
    13681 cacaaatttt tcttcgcctc gatatgtgtt ttctggtgtt ggtggtatta tgatttttgg
    13741 catctctatc actcctttag ataaatgtta aagtttgtta ttattcgccc tgtattgaag
    13801 ttctctatct aatgcataga aaactttgtt tatttctaag tagctgtaat cacttttttt
    13861 aataagctct aatatttccg ctcctaagtt acgttccttt tccgttaaat aggatgaaga
    13921 agcatcagct ttgctagaaa cttgtgggac gcctatacgc aatccttctg atcttgtgtt
    13981 catttgttta tgctcctttc gtgtataatg ttgttatcaa cctaaggagg tgataacatg
    14041 cccttgatat ctgatgaatt tgatacactt actaaagacc aacaatatat cttgtccgta
    14101 ctctacaaag attatttaga atgtgtaaag ttaggttcgg ttaaattaac ctgcaataat
    14161 tttggaagtg ctaaagatat acatacaaag tattttcaaa aactacattt cgaagatgta
    14221 aaatacgatt taaataaact taaaaactct gggttcctaa acggcgtgta tgctagtaac
    14281 actatttatc atgtaacaat ttcagacaag actgttgttt actttgaaaa tgagtttaaa
    14341 aacaatttaa aaagtatcat tgatagcatt tctaaaattg cttcaataat tcctggtctc
    14401 tagttgggtt tataacttcc caatcgtttg ccatgaggtc atcggctgaa ggttgccaat
    14461 atctgataag gtttgtccca tcgctatttg aaatgatgca ttgtaaaaaa ctatcatttg
    14521 ttggtaatat cttagttcga tgactttcct tccaatcttt ccgtgtcata gagacaagat
    14581 tttttgtagc tatcttagtt gcttcttgaa tgttcatttg ttattcctcc tttcgtgtat
    14641 aatgttgtta tcaacctaag gaggtgataa gtatgaaagc ttgtttatat ctttctaatg
    14701 ataaatttgt tgaaatcgat aatttagaaa aagtgataaa gtcaggtcat cgcggaactg
    14761 ttgaaatatc aaaagaaaaa attaaaagtt ccttgttcac taatggctca tatacttttg
    14821 ttggagacaa aatagtagct atcgcttcag ctaaaatcga attcatagaa tttatcgatt
    14881 aatctcttta agcaactctg caactgctcg caacagttca gggttgtttc ttgtttctaa
    14941 attactgttt gcatgtttta gtaaattgag ttttaattta cttttttctt tagcgattct
    15001 aaatttttgt aacatttgtt gttcctcctt ttaagatgtt tgtttaaatt tcaaattggc
    15061 taatatctac accgtattta atcgccatac tcttaatcac tgaaatgtat atctcaacca
    15121 atctaggttc atcagtaatc acatctaatt ttgacaactt gttaatctgg gttttcgttg
    15181 caccattcgc tagcattttg cctttgcggt tctgcatacg aatttttaaa ttacagcgtc
    15241 ctttttcttc taaagcttta tatgcttcag acttaacttt ctggtgcatt gctccgccac
    15301 ctaaatgttg tgcaatcgca gataacattt tgtttgtgtc gttacgccag tttttcgttt
    15361 caataccgac aatgtgacga atgcctgtga tttcttgttg catttgtttg ttaaactgtt
    15421 cttggtcttt ttgtgctttg aacatcatct ctaatgcttg cattggtgtt tgtggtacat
    15481 taagctgtgc ttgttgttta atgtattcat ccattttatg aaatgcatca acataagttg
    15541 cagtaaacaa aatgccttta ctacctgtca tcttgtttgc tactatgtcg caaccttttt
    15601 tggttagtag gtaacaaggt tgtactttgt tttgtgaatt aacataggtg ctttcttcaa
    15661 agaaattatg actactcaat tttgaggagt cctctaaaac cttgatataa cctttaatgt
    15721 ctcttactaa attgtcgtgt cgctttccta tcatttccgc aacttctcta ctgtctacat
    15781 aatgtgtttc gttctgttct actatttgta atgcttgcat ttcagtttcc tccttaagtt
    15841 aaaactttct ttttgcgtaa gtcttcgtta aaaaaaatat ctcttccttc ttgaggtgtc
    15901 aattctaacg caaaataaat accatttatt accgggtacg acggtttcgt tctcccgtga
    15961 atcatattag ataaagtatc tctattaaca ccaatttctt cagaaagggt tttgatgtta
    16021 tgttctttca aagccatttt agatttcaaa agtttagcat ctataggcat ttcttttcac
    16081 cacctttcgt attacgtaag taatcttatc atgatgttac gaaagaggtc aagcacttta
    16141 cgaaagtttt ttagaaaaat attgcaaatg ccgaaagttt tccttataat agaactatca
    16201 agtaaaagga gctgtattac gatgtgcttt tcaaaaagaa tgaaacaatc aagagaaaaa
    16261 caaggtatga ctttggccga actaggaaga aaaattggta aaactgaagc tactgtacag
    16321 cgttatgaaa gcggaaatat caaaaattta aaaaacgata ctatagaaag tatagctact
    16381 gcattaaatg ttaatcctgc atatttaatg gggtgggttg aagaaaacga tgatgaagta
    16441 caacatcgtg cagctcacct tgaaggagaa ttgacagatg atgaatggca aagagtttta
    16501 gattatgcag attatataag aagcaaacgt aagtaaagga tgtatcagat gggattatat
    16561 gaagaaactt taatacaaca tgattatatt gaaataagag aggctgatgt gcttccagat
    16621 aatttggatg gggtatggtt aggagattta attttaataa agcgtggctt atcagataga
    16681 gaaaaggcag gaattctctt tgaagaatta gcacataata aacttacata cggtgatata
    16741 gccgattact cgaaattcaa caatcgcaag ttcgaaaatt acgcaaggcg acacggcttt
    16801 atctcagcag tcccgttacg cgaaattgtg gaagcttaca attatggtgt acgtaacttg
    16861 tatgagttgt ctgagtatct gcaattgagt gaagaataca tattagaagc aatagaacaa
    16921 tataaaaaga tatatggtat tgggactcac tacggcgaat actcaattac atttgagcca
    16981 ttgagagttt ttaaatataa agaaatataa acaaaggaga aattaaaatg aaaagattat
    17041 taggtttaac attagcgagt gcgttagttt taggcgcttg tggtagccac gacggcgata
    17101 agaaagagga aagcaaaaaa actgaaacaa agaaagataa caaagataaa aagaaagaaa
    17161 ctaaagaaaa agcagaagcg aaaaaagaaa atgctaatca aaacgataac aataatcaag
    17221 taaacaacga gaacaacaca aacgttaaca acgatcaaca aaccaataga cctttaacta
    17281 aagacgaaat atcacaaaga gtaaaaaatg gtcacaatgt taacggcatg gtagatgcag
    17341 atggtaatac ttggtaccaa gcacaaggcg caggtgacgt tataggttac acaaaacctg
    17401 atggtacaca atgcacagtt ggtggttgtg tcacacctca gcaacaagaa caaataaacg
    17461 aagctaatta taaagagatg gaaaaatatg ggtattctcg tgaaaaatac gatgcaattc
    17521 aaaaagaagc ttctaaactt caacaacaaa aagaaaatgg agaaataaca gcagaagaat
    17581 ttactaatag gtatatagaa ttatacgact aagtatctta caatcaatta attgtattgt
    17641 gattaataac gt
//
LOCUS       DBNENW010000045        16871 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNENW010000045 DBNENW010000000
VERSION     DBNENW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.045"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            144..1025
                     /gene="rbsU"
                     /locus_tag="AC1LW8_001209"
     CDS             144..1025
                     /gene="rbsU"
                     /locus_tag="AC1LW8_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015591 - D-ribose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015752 - D-ribose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose/proton symporter RbsU"
                     /protein_id="HIP4773054.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            1040..1444
                     /gene="rbsD"
                     /locus_tag="AC1LW8_001210"
     CDS             1040..1444
                     /gene="rbsD"
                     /locus_tag="AC1LW8_001210"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIP4773055.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            1472..2386
                     /gene="rbsK"
                     /locus_tag="AC1LW8_001211"
     CDS             1472..2386
                     /gene="rbsK"
                     /locus_tag="AC1LW8_001211"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIP4773056.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKSTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(2484..2618)
                     /locus_tag="AC1LW8_001212"
     CDS             complement(2484..2618)
                     /locus_tag="AC1LW8_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773057.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            2637..3398
                     /locus_tag="AC1LW8_001213"
     CDS             2637..3398
                     /locus_tag="AC1LW8_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIP4773058.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPITIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(3779..5215)
                     /locus_tag="AC1LW8_001214"
     CDS             complement(3779..5215)
                     /locus_tag="AC1LW8_001214"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIP4773059.1"
                     /translation="MTKLPRNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITKSIDQNHYYPNHEGIDFYHRYKEDIALFNEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQDIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(5231..6022)
                     /locus_tag="AC1LW8_001215"
     CDS             complement(5231..6022)
                     /locus_tag="AC1LW8_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIP4773060.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YIPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVRIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            6171..6875
                     /locus_tag="AC1LW8_001216"
     CDS             6171..6875
                     /locus_tag="AC1LW8_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP4773061.1"
                     /translation="MLKYEHIAKQLNAFIHQSKFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(6983..7684)
                     /gene="lrgB"
                     /locus_tag="AC1LW8_001217"
     CDS             complement(6983..7684)
                     /gene="lrgB"
                     /locus_tag="AC1LW8_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HIP4773062.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(7677..8120)
                     /gene="lrgA"
                     /locus_tag="AC1LW8_001218"
     CDS             complement(7677..8120)
                     /gene="lrgA"
                     /locus_tag="AC1LW8_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HIP4773063.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPGSV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(8233..8973)
                     /locus_tag="AC1LW8_001219"
     CDS             complement(8233..8973)
                     /locus_tag="AC1LW8_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HIP4773064.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(8976..10730)
                     /locus_tag="AC1LW8_001220"
     CDS             complement(8976..10730)
                     /locus_tag="AC1LW8_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HIP4773065.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(10974..11648)
                     /gene="scdA"
                     /locus_tag="AC1LW8_001221"
     CDS             complement(10974..11648)
                     /gene="scdA"
                     /locus_tag="AC1LW8_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HIP4773066.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(11792..13513)
                     /gene="tarS"
                     /locus_tag="AC1LW8_001222"
     CDS             complement(11792..13513)
                     /gene="tarS"
                     /locus_tag="AC1LW8_001222"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIP4773067.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     TLLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(13546..15234)
                     /gene="tarL"
                     /locus_tag="AC1LW8_001223"
     CDS             complement(13546..15234)
                     /gene="tarL"
                     /locus_tag="AC1LW8_001223"
                     /EC_number="2.7.8.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_function: GO:0047356 - CDP-ribitol
                     ribitolphosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid ribitol-phosphate polymerase TarL"
                     /protein_id="HIP4773068.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(15256..16281)
                     /locus_tag="AC1LW8_001224"
     CDS             complement(15256..16281)
                     /locus_tag="AC1LW8_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HIP4773069.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(16274..>16871)
                     /locus_tag="AC1LW8_001225"
     CDS             complement(16274..>16871)
                     /locus_tag="AC1LW8_001225"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HIP4773070.1"
                     /translation="ILINDFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDR
                     NDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAI
                     DTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIV
                     ETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD"
BASE COUNT         5410 a         3158 c         2552 g         5751 t
ORIGIN      
        1 gaaattaaaa taataatgta tgaattttta aatatgattt aaacgttttc attttttatg
       61 aaaacgcatg cattttacaa ataaaaatgg tacgatggca ctggtaaaac gttttactaa
      121 aaacaaatcg tgaggtgtat aacatgagca ttgttgcatt acttatcggg ttaggcccct
      181 taattggctg gggcttcttc ccaacagtcg cttcaaagtt tggtggtaaa cctgtacatc
      241 aaattatcgg tgctactgta ggtacgttaa tcttcgctat tatattagcc gtagtcacat
      301 caagtggctt ccctactgga accaatttgc tattcgcctt attatcaggt gcaggatggg
      361 gattcggaca aatcattaca tttaaagcgt tcgaattagt cggctcatct cgtgccatgc
      421 cagtcacaac agcattccaa ttattaggcg catctttatg gggtgtcttt gcattaggaa
      481 attggccagg cattggtcat aaaatcattg gatttacagc tttagtcgtt attctaattg
      541 gagcgcgtat gacagtttgg agtgaacgca aagaagcaag taacgccaaa aatttacgtc
      601 gtgcagtggt acttctgtta attggtgaat ttggatactg gttatattca gctgcaccgc
      661 aagcaacttc tattgatggc ctaactgcct ttttacctca agcaatgggt atggtaattg
      721 ttgcagtcat ttatggcttt atgaatatga aagcagagaa tccattccgt aataaaatta
      781 cgtggttaca aattatttca ggtttcttct ttgcatttgg tgctttaaca tatcttattt
      841 cagcacaacc taatatgaat ggtttagcaa ctggatttat tctttctcaa acatccgttg
      901 tgcttgctac attaactggt atttatttct taaaacaaca taaaacgtca aaagaaatgg
      961 ttattacaat catcggctta gtactcattt tagtagccgc ttctgttaca gtatttataa
     1021 aataaggagt gtagatgtca tgaaaaaatc agctgtttta aatgaacata tttcaaaagc
     1081 aatcgcgaca attggtcatt ttgatttatt aacgattaat gacgctggca tgccaattcc
     1141 aaatgatcat cgtcgtatcg acctagctgt aactaaaaac ttaccacgct ttattgatgt
     1201 cttagctaca gtgttagaag aaatggaaat ccaaaaaata tacttagcag aagaaataaa
     1261 agaacataac cctacacaat tgcaacaaat taaacaattg atttcatcgg aaatcgaaat
     1321 cattttcatt cctcacgaag aaatgaaaag taacttagct cacccattaa ataaaggtaa
     1381 tattcgtact ggtgaaacaa cgccctactc taatattgca ttagaatcga atgttacttt
     1441 ttaaaagtta taacttgaaa ggagcataca catgaccaac aaagttgtta ttttaggttc
     1501 aacgaatgtc gatcaatttt taacagttga aagatatgca caaccaggcg aaacattaca
     1561 tgttgaagaa gcacaaaaag cattcggcgg aggtaaaggt gccaaccagg ctattgccac
     1621 tgcacgtatg caagcagaca caacatttat tactaaaatt ggcactgatg gcgttgctga
     1681 tttcatctta gaagatttta aagcggctca tattgataca tcatatatta tcaaatcaac
     1741 tgaagcaaaa acaggccaag cctttatcac tgtgaatgca gaaggacaaa acaccatcta
     1801 tgtttatggt ggtgcgaata tgacgatgac acctgaagat gttattaacg caaaagacgc
     1861 tataatcaat gcagactttg tcgttgcaca attagaagta cccatcccgg ctattatatc
     1921 tgcatttgaa attgccaagg cacatggtgt gacgacagta ttaaatcctg caccagcgaa
     1981 agcattacct aatgaattat tatcattaat cgatattatt gtgccaaacg aaacagaagc
     2041 cgaattgtta tctgggatta aagtaactaa tgaacaatct atgaaagaca atgccaatta
     2101 ctttttatct ttaggcatta agactgtttt gattacgcta ggtaagcaag gtacatattt
     2161 tgctactaaa aatcaaagcc aacacatcga agcttataaa gtaaatgcga ttgatacaac
     2221 tgctgcaggc gacacattta ttggtgcatt tgtcagtcgc ttaaacaagt cgcaagataa
     2281 cttagcagat gctattgatt ttggtaataa agcgagctca ctcactgtac aaaaacacgg
     2341 cgcgcaagca tctattcctc tactagaaga agtaaatcaa gtttaaatga agcaaacaca
     2401 gctatgatat gaaggtttag catataacat gcaacattcg tatatcatgg ctgtgctttt
     2461 ttatctttat aaaacatcat ctattagaaa taattttcca ctaaacctat gcttgttgac
     2521 tcatgtttag ttataaatga agtgacaatt ttttgtaatc tttttaactt ccaaattatt
     2581 gcatataaat atgctatatt aatgataata attatcaatt aaaaggaggt tatgctatgt
     2641 ctaaagaagc tggtcataca tttttagcta aattaggaaa aactcgtcta cgccccggtg
     2701 gtaaagaagc aacagattgg ttaatacaac aaggggcatt ttcacaagat aaacaagtgt
     2761 tagaagtggc atgtaatatg tgcacaacat ctatttatct agctcataca tatggctgtc
     2821 acattcaagg cgttgatata aataagaaag cattagaaaa agcacaggaa aatatttcag
     2881 cagcaggtct tgaatcatat attcaagttc aacaagcgaa tgctgttaaa ttgccctttg
     2941 atgacaatca attcgatatc gttttaaatg aagcaatgtt aacaatgtta cccatcacca
     3001 taaaggaaaa agcattacgc gagtactacc gagtcttaaa gcctgggggt atcttgttaa
     3061 cacatgatat tgtcatcgtt aatgaatcac atgccacaca tgtagttaaa tcattatctg
     3121 cagcaattaa tgtcaatgtc tcaccgcaga cgaaacttgg ctggttagat ttatataatc
     3181 aagctggttt taatcatgtg cattatcata ctggtccaat gagtttaatg acaccaaaag
     3241 gtttaattta tgacgaaggt attgttggaa ctataaagat tatcaacaat gctttaaaaa
     3301 aagaaaatcg accaatgttt tgtaaaatgt ttaaaacgat gactaaattg cgtaaagata
     3361 tgaattatat tacttttgtc gctaaaaaag aggactaaat ataatgccac taaccgtact
     3421 ttgtatctat gtttgactgc tactttaatt tctttgtgac actaatcatc tacttaacaa
     3481 tatcgttatc gttgattagt aagtcatcaa ttttggttaa agactttcat aaacactcaa
     3541 acattaacac tatacatact tagttagtgg cattattttt ttaaaaattt taacatcacg
     3601 ggattggaac agaaatgata ttttcgcaaa atttattcct gtccttcccc aacttgcaca
     3661 ttattgtatg ctgactttcc gccagcttct atgttggggc ccgccaatat aacactgtag
     3721 gtcctactat attaatttat gtctcgggct ctaaccctaa aatacttata atataccttt
     3781 ataatttatc tccatttgat gctatcactt gttgatacca atagaacgaa tcttttttca
     3841 agcgttccat cgtgccatga ccatcattat ctcgatcaac atatattaaa ccatagcgtt
     3901 tcttcatttc accggttgta aatgaaacaa tatcaatgat tccccacggt gtataaccga
     3961 ttaaatctac accatcttga tcaactgctt ctatcgctgc tgtaatatgt gcttttaaat
     4021 attcaatgcg ataatcatca tgaatatgtc catctaccac ttcatcaact gcaccaaaac
     4081 cattttccac aataaaaagt ggtaactgat aacgatcata taacacattc aatgtatatc
     4141 ttaagccatc tggatcaatc gcccaacccc aatcactcgt tgcgatatgc ggattctcca
     4201 cagaatgatt caaaccaccg ttgacgatgt tgttttcaac tgtagtatca acatcatgtt
     4261 ttacagccgt agacatgtaa taactaaagc caatataatc aaccgtgccc tgctgtaaaa
     4321 tactgtcgtc cccttcttgc catccaacat catatccttt tcgtgccaac atttttttag
     4381 catagcttgg ataataacct ctcacttgga catccgggaa aaagaaacgt aagcgattcg
     4441 caatttgtgc ttccatcata tctttcggat gacacgaata tggataaatg ggtacatgtg
     4501 aaatcattgt tccaatctta aacttcggat taatctcttt accaagacga actgctaaag
     4561 cactggctaa aagttcatga tgtgctactt gatacaagac ttcttcagga ttatcatttt
     4621 ctgtcaatgc taccccagaa ttcgtccata aaaagatagg atttgatgtg tccatctgat
     4681 tattaatttc attaaacgtc atccaatatg tcactttatc tttatatctt tcaaatacaa
     4741 cacgcgcaaa atgcacaaaa taatcgacaa cttctctatt tctaaatcca ccataatgtt
     4801 tcgctaaatg aagtggcatc tcaaaatgtg ataacgtcac aacaggttca atatcttgtg
     4861 caattaattc atcaaagata cgatcataaa aggcgagtcc ttcttcgttt ggcacatctt
     4921 catccccatt cggaaagata cgtgtccacg caatcgacgt acgtaaacat ttcaatccca
     4981 tttcattaaa caaggcaata tcttccttat aacgatgata aaaatcaata ccttcatgat
     5041 ttggataata gtgattttga tctatagact ttgtaatctg acgtgctttg ccatgtgcac
     5101 cactcgtcat aacatcaatt acacttaacc ctttaccccc tttatcatat ccaccttcaa
     5161 attgatttgc ggcaagagcg ccaccccaca taaaatttcg cggtaatttg gtcattatta
     5221 caacacactc ctagttttta aatattttaa aaataacatc ttcagtcgta atgatttcat
     5281 tcattgtcag ttctactttt cctaaatcgg cagagttgct aataacgaca ataacgtcag
     5341 cattgtagcc ttgttgttgt atatattgct ggtcgaattg taacaacgtt tgccctgctt
     5401 caacacgatc attttgcttt acaaagcact tgaacccttt accttccaag tcaactgtat
     5461 tcacgccaat atgaatgact atgtccacac cgtcttctga ttgaatacga actgcatgct
     5521 tagttggtac aatcatagat attaaaccat tgaacggtgc gattactttg gattcttcat
     5581 gagctttgat tgccaagcct tcgcctacca ttttctctct aaaaattgaa tctcttactt
     5641 ctttaagtaa aatacgacga ccagcgattg gtgctttcac taaaattggt gttgttactt
     5701 ttacttgagg tatataactt gtatcttggc tattaatatt gtcagtattc tcactctctt
     5761 cgacattagc attcttaatt aatcgctgta attgctgata cacttcatcg acatttccat
     5821 ttaattttat gactaactgc gcatcatttt ctgctacaga tgtcacatca actatttgtt
     5881 tcacttcatt tgctgtagaa ggaattgtat gttgcatatg aattgtaatg cacttcgtgt
     5941 catacgttgc atcaacaatg ttttcgacac cacctacagc tgtaagaatg tcttgggctt
     6001 gttctttata tttattgctc ataaatatta ccccctcaac ttgatagtag aatgaaaatg
     6061 tatttactta cctccaatta taattgtacc ggttcaattt gtaaacgccg atacaattat
     6121 aatattttgt gctataataa ttacagaaaa agtgaaacga ggacagaata ttgttaaagt
     6181 atgaacatat tgctaagcaa cttaatgcgt ttatacatca atctaaattc aaacccggtg
     6241 ataaattgcc aagcgtgacg caattaaaag aacgttatca agtaagtaag agtactatca
     6301 ttaaagcatt aggcttattg gaacaagatg gtttgatcta tcaagcacaa ggcagtggta
     6361 tttatgtgag aaatattgca gatgccaatc gtatcaacgt ctttaagact aatggtttct
     6421 ctaaaagttt aggtgaacat cgaatgacaa gtaaggtact tgtttttaag gagatggcaa
     6481 cgccacctaa atctgtacaa gatgaactcc aattaaatgc agatgatacc gtctactatt
     6541 tagagcgatt aagattcgtg gacgatgatg ttttatgtat cgaatattct tattatcata
     6601 aagaaatcgt gaaatattta aatgatgata ttgctaaggg ctctatcttc gattatttag
     6661 aatcaaacat gaaacttcgt attggttttt cagatatttt ctttaatgta gataaactca
     6721 cttcaagtga agcttcatta ctacaattgt ctacaggtga accatgttta cgttaccacc
     6781 agacttttta tacaatgact ggcaaaccct ttgattcgtc tgacatcgta tttcattatc
     6841 gtcatgcaca gttttatatt cctagtaaaa agtaataaat acataaaaac gtctatatcc
     6901 cagttataaa ctggagtata gacgtttttt tacgataata acaatggctc aaattgctat
     6961 tatcttgctt aggtttttcg ttttagaaga atattgctac aaagacaggc acaactgcta
     7021 caacaactac accaactaac actaaagcta tacttgccat tgattcttct acaggtccta
     7081 attctttggc tggtgctaca cctaatgtgt gaccacttgt tccaagtgct aatcctcggg
     7141 caatagggtt agtaattcgg aaaagcttca agaatttatt acctagggca taaataatga
     7201 caccatttaa aataactgct aatgatgtta attcttttat accaccgata ccagctgata
     7261 ctggtaacgc aatcgctgta gttgctgctt gaggtaacat tgataaaata acatcattgg
     7321 caaattgtgc taacttcgca aaagttaaaa taattaataa cgctacaact gtaccgatac
     7381 caatacctcc gatgatacga tgccaatgtt taacaagcac ttcacgcttt ttatataacg
     7441 gaatcgcaaa acagattgtt gccggttcta agaagaagta aataatgtct ccacctattt
     7501 tgtaagtctt atacggaatg cctgttaaat agaggaaggc cacaccaaat accatactga
     7561 caaatagcgg tgcgaataag aagaaacgat tagttttttc aaataatatg gtcgctaaga
     7621 aaaatggtat aacggataac agtattccga agtaaggtgt atttagtgct aagtggttaa
     7681 tcatgagctt gtgcctcctc tattttgatc ttttttgtga ctttgtcacc tttagatctc
     7741 gaagtaactt tcataataat ttgtgtgaca tagccagtac aaataagtaa tagtattgtt
     7801 gagacgatta ttagtccaat gattaaaaat ggtgcttggc taatgacacc taaagagtta
     7861 acaactgaga taccggctgg tacgaagagt aagccaatgt tatttgttag tgtcgttcct
     7921 actttttcga cttcgcctaa cttaacagca ccagtacata ataatacaaa taataatact
     7981 aaaccgatta ctgatccagg cataggaatt ggcataaatg attcaattat tttcgataca
     8041 aagagtacta aagcaattac aatgacttgg tgaaaaaagt gtgctggttt tgatgcgtct
     8101 ttttgttgtt tcacgaccat tgcctcctac gtttgattta actaaagtat agatggctca
     8161 cttcgatttg cgtaaatttt agtccgaaat acaaaatatc ataggtaaaa tgcataaaaa
     8221 aaaggattac tgttaaagta accctatcga cgctttaaaa tctttcataa atgaacgtcc
     8281 aacttgcatc ttgacaccat ttgtcaatat taccatataa gtgtagttaa accattgttg
     8341 cacttcttta atgtgtttcg tattaataat atatgaacga tgaatacgta tgaaataagc
     8401 gggattcaat cgtttttcat aacgattcaa tggctctgtt gtttcgtatt tatgattcgt
     8461 tgtatgtatg gttgtaatac cattatgtgt gccaatccca ataatatttt gttgctttaa
     8521 catgtgaatt ttatcgtcta tttcaacagg taaactttga tcaaaattcg ccgacatatc
     8581 attcgcaatt gcacttgcgc tattatcatc tttggcttta gtcgcacgca ctttattgac
     8641 tgcttgttca atacgttttt gaccaaacgg tttcaaaata tagtctgtcg catttaattc
     8701 aaatgcctgt actgcgtatt ggtcatgtgc agttgcaaaa ataatcgcag gtggctcttt
     8761 catcttttga atcttagctc ctaattcgat cccattttca tccattaaat tgacatctaa
     8821 aaatataatg tcatattgat tgatcagtag tgcttccaat gtttctttta cattttctgc
     8881 ctcattaatt tcttcaaaac caccaatttc atttaataaa tatgttaatt cattacgtgc
     8941 taatggctca tcatctatga ttaatgcttt catatttatt cctcctcttg tctttcataa
     9001 ggaagtacac accaaaaagt ggtaccgctc gatgtcgatt caaattgtaa tgctgcggat
     9061 tttccaaata atccttttag gcgtaagttt aaattttcta aagcactacc agttccagac
     9121 tctgattcta cagatgtttc tcccaacaaa tgcattttat ctttagaaat accctgacca
     9181 ttatcttgta caataatacg tacatgtgtt gcagtttctt taatcactga cacgtcaata
     9241 tcgttacctt gctttcgatt cgtaaacgca tgtttgatgg cattttcaac taaaatttga
     9301 attaaaaatg gtggtacaag cacatcgcga tatttgtctt caacattaat attgatatta
     9361 aatcttcctg gataacgtgc ttgttcgagt gataagtatg cacgcacttg acttaactct
     9421 ttatctaaag taatcgtatg ttgcttagag ccttgtaaat tcgctctgaa aaaataactc
     9481 aattctaata gtaactctcg tgccttttcg ctatttattc taactaaagc tgaaatcgtg
     9541 ttaattgaat tgaagaaaaa atgtggactc acttgtgcct gtaatgactt aatctcagca
     9601 tctttcaata acttactttg cgtttcggct tcaccaagtt caatttggct actaaaaata
     9661 tttgccaatc cttctgcaag ttgacgttcc acaaaagtta aatcattagg gtttgtaaaa
     9721 tacatcttca atgtaccgac gatagaacca tgcatctcaa gtggtatcac gatagctgct
     9781 ctaagcgggc aattcggatg actacaacca atctcttctt tagtatgaac ttctttcaac
     9841 tttcctgatt tcaatacatc tttagacaga cttgttaata tttcatttgt tggtatgtga
     9901 tgatcactac ctgcacctac atgcgataag atttcatttt tgcttgtaat tgctacggca
     9961 gatactttca ttaaattttt aataatcatc gcaatttgct gtgccgattc tctattcaat
    10021 ccttctttaa aatacggcaa tgtctggttc atcaattgca gtacatcatg tgtttgaaca
    10081 gccttcattt gctcctcttg ctttaatgtt gaaatgataa tagacataaa aatcgccgta
    10141 ccgacgctat taacaataat cattggtagt gcaattaatg atatgaggtc aaccgcatat
    10201 gctttgtcgt gggaaaatgt taaaatgctc aacatttgaa tcatttccat aacaattcca
    10261 atcatggcac ttttcgcaat actcgggtaa cgcttgcgtc tttgagcttg taagccaaaa
    10321 taaccagcaa ttataccgat aaatatagat gagataagat aaacttgtgc atccgcccca
    10381 cccatataca ctctgaaaat acctgaaata acgccaacaa atagacctac aaaagggcca
    10441 ccaactaatc ctgcgacacc tatcgttaat acacgtgtgt tagctaaaga tacatcatca
    10501 tctaaacgga agtacacact tcctgacaaa ctatgttgat gatcgatgac gataccagtt
    10561 aaattagaca ttaaggcaaa caaactgaaa ataatacata attgccaacg tgctttccat
    10621 gtacgtcgac gattcattaa gtttttaaaa tatggaatat tcatcaacac ataggccaaa
    10681 ataataatta aacctacacg ctcaagtaat aacattgtta gcgatagcac gatagcacct
    10741 cagtaaatta taattatgtt tattttatca taatagtgca tgtaaaattc atgaataaat
    10801 tattaaaaaa cacgctacta tttaattatc tataccatta atttttcata tcaattttta
    10861 tgctaagttg accatttttc ttttcaaata atgctgttca tatcactaaa tttctgttaa
    10921 gtatagcgaa aaactgctga cattactttg ccaacagttt ctaatcgcgt tatttatgat
    10981 acttttttaa ataaaacatg attctctaaa tgaacatgct catgtgttaa cacttcaagt
    11041 gcttttaatc tctgataaac gagtcgccac gtaccacatg cctctatcgg tggttcatag
    11101 ctagatgtta aatcgctcat tttcactaac aattgtcccg ttgcaatgtg atcagatact
    11161 aaatcatcaa tcactgtatt aatatcgtct actacttcac cttgttcata tttaattagt
    11221 tttggaaaat caacatcatc ttctttttgc atatgctcta acatgccatt tttaaatgta
    11281 tcatatgttt cttttaactc gactaaatat ggatggttag gtccatgtac tttcgataat
    11341 ttcgtcacat aaggtgttaa atttttaaat tcttctctaa gaggttcatg atatgctgct
    11401 tgaatatatt gaataagtga cgaaacattt aaaaatttag ggttaagcga acctggtgta
    11461 ttcgtttgtt caacgtcatt gagacgctgt aataattcgt tcaaatctac atttttcttt
    11521 tctaaggatg ctgcttctat acttacttgt ccaccgcaac aaaagtctat acctacgctt
    11581 ctaaaaatat ctgctgcttt cggataatct gttactacat ctgctactat gtcattttta
    11641 tttatcatta tagacgacct cctcattttc agtgtataca atttaatgta tatgaaaatg
    11701 aaaatcaggg aaatgtcata atcatttcat aaatagtaaa ataaaaccct caaatcatgt
    11761 tagttcacat agatttgagg gcatttatta tttattttag cgagtaagtc atatgtgcag
    11821 tgtttaactt aacaatattt ttgaagtctc gcttcttaat atctttacta agtaagattt
    11881 ccacttcgtt ttctgacgtt tggcttaaca ttcccataat catgtcgtct tttagcacga
    11941 cactagccat accttcttca aatacataac cacgatccag accagttaaa atgattttgt
    12001 ttttaccttt taacttcaat gagattgaat ccgttaatgt aatagctgta aagtataacg
    12061 aaatgttatt aaagtctttt gtgaaatacg gtgttaatct ataaaaatca ttattcaaat
    12121 gaattaatgc acttgtttct gcagtatatg catatttatt acgttgatta ccaacacgca
    12181 cttcaaatac ttccgtacct atttggactt ctaagaaaac atcccaaatt ttttctttag
    12241 gtaaatacgc tttaatatct tctaacataa tgttaaaacg atattgttta ttcgcattga
    12301 cacttaaagg tacatacatt gtgtgcttct tgtttcgtga atgtaattta agtctgacat
    12361 cataatgtcc ttcgccaatc ataaattcat ttaacgttag ctctgtaaca attttatgat
    12421 tatcaaattt aaatgctgtc attttcactt ttggcttagc gatatcaatg ccttcaaagt
    12481 atggttcgcc ttcattgaat tgaatgttta atttaccatc tacaatatca aaacggtagt
    12541 attgaccttg acggtaactt tcttctacag ttctatagtt gtctatatct ttcgctctcg
    12601 cgtagtgcaa caatggtcgt aatttactca tcaccaatgc atctacacgt tctggaactg
    12661 cttgtataaa gtctcctaga gcattaatcc attgcggttg atcttctagt ttaactttaa
    12721 gtgagaagcc attcgtacga gaaaaactaa aatgacgatt taaaaattct gctaagattt
    12781 gatctttatg agcttcttct aaatctgcat ttaatatttc tacagcaatc aatctcatga
    12841 cttcgtaaaa gtcttcaggt gaaacatacg cactactcat atgttcacct tcacgctttg
    12901 tagcataata atacgcttta tcacttaaca cactgattcg atttgcattc aaatatgctt
    12961 tcattgtaaa taattgatct tctgcactct ttaattcttc tggaaaataa atgtcattat
    13021 cttttagtaa tgttgttcta tagattttag tcgggcttaa agtatagata attcttgaat
    13081 ttaacaaagt aacttcaggt gctgtttctt taaacataga ttgtggtaca ccacgaccat
    13141 taacaccttt cattttaatc aataagacat cggagtgatg ttcatcaata aatgctgctg
    13201 catcttttaa agtctctttg tttatatagt catcggagtc cacaaataat acaaattcac
    13261 cttctgcttg ttttaacgcc acatttctag gtttacctgg accaccagaa tttgtttcga
    13321 gttgacttac tttcaagttc aatttattgc gatacttttc aactatttgt aacgtttgat
    13381 ctgttgaaca gtcatcaact acaaccactt caaattcagt tttcggaaaa tcttgtttcg
    13441 caaggctatt aagtaattct gttatatact tttctgaatt gtatgttgga actattactg
    13501 aaaatttcat cattatacct ctcccacttt gactactata taaacttagc taccaaataa
    13561 atttctgact aaacgctcac ttgatcggcc atcttgatat ttaaaatgtt tatctaagaa
    13621 tggaatgact ttttctcctt cataatcttc attgtccaag gcgtccatta atgcgtcaaa
    13681 tgattgcaca attttacctg gaacaaatga ttcatatggt tcataaaaat cacgcgtcgt
    13741 aatataatct tctaaatcaa atgcatagaa aatcattggc tttttaaata ctgcatattc
    13801 atatattaaa gatgaatagt cactaattaa taaatctgtt atgaacagta tatcattaac
    13861 ttctctaaag tcagaaacgt caacaaaata ttgtttatgt ttgtctgcaa tattaagtct
    13921 atttttcaca aatggatgca ttttaaataa tacaaccgcg ttattttttt cgcaatatct
    13981 tgctaaacgt tcaaaatcaa ttttgaaaaa tgggtaatgt gctgtaccat gaccactacc
    14041 tctaaatgtt ggtgcgaaaa gaatgacttt cttaccttta ataattggta attcatcttc
    14101 catctcttgt ttgatctgtg tcgcataagc ttcatcaaat agtacatcag tacgtggaac
    14161 acctgtaggc actacatttt tctctttaat accaaatgct tcagcgtaga atggaatatc
    14221 ggtttcagat gatacataag cttttgtata gctacgatga tttaatgaat caataaatgg
    14281 tccacccttt ttaccagtac gactaaagcc aactgtttta aaggcaccaa cggcatgcca
    14341 tacttgaata acttcttgag aacgtctaaa acgcactgta taaatcaatg ggtgaaagtc
    14401 atcaacaaag atgtagtctg ccttcccaag taaatatggc aatctaaact tgtcgatgat
    14461 gccacgtcta tctgtaatat tcgctttaaa aacagtgtga atatcatact ttttatctaa
    14521 attttgacgt aacatttcgt tatagatgta ttcaaagttt ccagacatcg ttggtctaga
    14581 gtctgatgta aacaacaccg tattcccttt tttcaagtgg aaaaatttcg tcgtattaaa
    14641 tatcgcttta aaaataaatt gtcttgtatt aaatgattgt ttgcggaaat acttacgtaa
    14701 ttctttatat ttacgaacga tataaatact tttaacttcc ggagtcgtta caacaacatc
    14761 aaggacaaat tcattaacat cgctagaaat ttcaggtgta acagtataaa ccgttttctt
    14821 cgaaatgccg ccttttctaa attcttttag gtaagtctgc aataagaaat tgattttacc
    14881 attttgtgtt tctaattcgt tgtattcttc ttcttgttct ggctttagat tttgatatgc
    14941 atcattaatc acatctgggt ttaactgtgc aatataatca agttcttgct cattcactaa
    15001 taagtactta tcttcaggta agtaataacc attatctaag atagctacat tgaaacgaca
    15061 aacgaattga ttcccatcta ttttgacatc attcgccttc attgtacgtg tctcagttaa
    15121 atttcttaat acaaaattac tatcttctaa atctaggttt tcactatgtc cttcaacgaa
    15181 taactgaaca cgttcccaat agattttatc tatatatatc ttacttttaa ccaacgttaa
    15241 ttcatccttt tctatttaca taatccattt taatactgtt ttaccccaag atgtagacag
    15301 gtctgcttca aaagcttctg taagatcatt aattgttgca atttcaaatt cttgaccttt
    15361 taacaacgct aatttatcta caatatctgg gtattgaatg tataagtcta caacatcttg
    15421 gaaatctttt gaaccacttc gactactacc aatcaacgtt agtccttttt ccaatactag
    15481 acgtgtatta acttctactg ggaactcact tacacctaac agtgcaatgc ttccttctgg
    15541 tgaaatgtaa tcgatcattt gatttatggc tgattgacta ccgcgaccac ccacacactc
    15601 aaatgcatga tcaaatgtta agccttcagg tattttatta ataaagaaga catcatcaac
    15661 aaatgagaag tgactcaatt tataatctgt tttaccaaag acatatattt tagactctgg
    15721 atataattta cgtaataaaa tggctgtaat gtaacctaag ttaccatcac cccaaatacc
    15781 aaatgtattt ttatttgaaa tagatttctt ttcaaaacga cgaatagcat gcaaacttac
    15841 tgttacaagc tctgtatatg aaataatact taaatcaata tcatcaggta gtggtacagc
    15901 tctatcatga tttagcaaca caaaatcttg cataaaccca tcatgtccac ttgatctgaa
    15961 gtagctcgat tttaaatagt tttcagcaat gacatcgtct ttttctgtcg gcgtattcgg
    16021 taccataact acttttgtac ctttattaaa cacaccttta ctgtcaaata cgacctcacc
    16081 aacaccttca tgaattaaag acattggcaa tttctgagat aagacattct catcacggct
    16141 accagtataa tatctttgat cggcagcaca aattgacata tataaaggac gtacaatgac
    16201 atagtcactg taaatatcta cgttgttata cgtaacttca aattgtctag gtgcaactaa
    16261 ttgatatact tgattaatca tcggcaatac cacctcgaat aatagcattc gctactttta
    16321 aatcgtaagg tgttgttact ttaatgttat ataactcacc ttttacaagt cgaaccggtt
    16381 tgtttgtttc tacaataatc ttacaagcat cagataaaat actcttttgc tcatcactca
    16441 actgtgcata gctttctttt aataaattaa tattaaacga ttgaggtgtt tgaccttggt
    16501 acatttcatt acgcactgga attgcatcaa tcgtttgatt atctttagat gtaacaatcg
    16561 tatctatagc atcaatcact gtatctactg caccgtactc taaagcagct tgaatatttt
    16621 ctttaataat acgatgcgtt aaaaatggtc taactgcatc atgtgtcaca atgacatcgt
    16681 catcgttaat accatttgtt gattcaatat gtttaacgat attcataatt gtatcgttac
    16741 gatcgctacc accttgaatg acttcaattc tttcatcaga aattttgaat tttctaagtg
    16801 tatctttcgt atgcgtcatc cattgttgtg gcgtcgcgat aataattttt tcaaaatcat
    16861 taattaaaat a
//
LOCUS       DBNENW010000046        16731 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNENW010000046 DBNENW010000000
VERSION     DBNENW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.046"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(208..1137)
                     /gene="mntC"
                     /locus_tag="AC1LW8_001226"
     CDS             complement(208..1137)
                     /gene="mntC"
                     /locus_tag="AC1LW8_001226"
                     /EC_number="7.2.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015410 - ABC-type manganese transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese ABC transporter substrate-binding
                     lipoprotein MntC"
                     /protein_id="HIP4773071.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(1134..1970)
                     /locus_tag="AC1LW8_001227"
     CDS             complement(1134..1970)
                     /locus_tag="AC1LW8_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP4773072.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(1964..2707)
                     /locus_tag="AC1LW8_001228"
     CDS             complement(1964..2707)
                     /locus_tag="AC1LW8_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP4773073.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            2829..3473
                     /locus_tag="AC1LW8_001229"
     CDS             2829..3473
                     /locus_tag="AC1LW8_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIP4773074.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(3553..4305)
                     /locus_tag="AC1LW8_001230"
     CDS             complement(3553..4305)
                     /locus_tag="AC1LW8_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIP4773075.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYLMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            4541..5305
                     /gene="tarA"
                     /locus_tag="AC1LW8_001231"
     CDS             4541..5305
                     /gene="tarA"
                     /locus_tag="AC1LW8_001231"
                     /EC_number="2.4.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047244 -
                     N-acetylglucosaminyldiphosphoundecaprenol
                     N-acetyl-beta-D-mannosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminyldiphosphoundecaprenol
                     N-acetyl-beta-D-mannosaminyltransferase TarA"
                     /protein_id="HIP4773076.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(5366..6160)
                     /gene="tagH"
                     /locus_tag="AC1LW8_001232"
     CDS             complement(5366..6160)
                     /gene="tagH"
                     /locus_tag="AC1LW8_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HIP4773077.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            6507..7319
                     /gene="tagG"
                     /locus_tag="AC1LW8_001233"
     CDS             6507..7319
                     /gene="tagG"
                     /locus_tag="AC1LW8_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HIP4773078.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            7418..8521
                     /gene="tarB"
                     /locus_tag="AC1LW8_001234"
     CDS             7418..8521
                     /gene="tarB"
                     /locus_tag="AC1LW8_001234"
                     /EC_number="2.7.8.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid glycerol-phosphate primase TarB"
                     /protein_id="HIP4773079.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKTMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLNPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            8518..9579
                     /locus_tag="AC1LW8_001235"
     CDS             8518..9579
                     /locus_tag="AC1LW8_001235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP4773080.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLPRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHTILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            9642..10040
                     /gene="tagD"
                     /locus_tag="AC1LW8_001236"
     CDS             9642..10040
                     /gene="tagD"
                     /locus_tag="AC1LW8_001236"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HIP4773081.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(10157..11452)
                     /gene="pbp4"
                     /locus_tag="AC1LW8_001237"
     CDS             complement(10157..11452)
                     /gene="pbp4"
                     /locus_tag="AC1LW8_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HIP4773082.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            11873..13600
                     /locus_tag="AC1LW8_001238"
     CDS             11873..13600
                     /locus_tag="AC1LW8_001238"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773083.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYNIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            13957..15186
                     /locus_tag="AC1LW8_001239"
     CDS             13957..15186
                     /locus_tag="AC1LW8_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIP4773084.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GMIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            15441..15533
                     /locus_tag="AC1LW8_001240"
     CDS             15441..15533
                     /locus_tag="AC1LW8_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773085.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            15711..16544
                     /locus_tag="AC1LW8_001241"
     CDS             15711..16544
                     /locus_tag="AC1LW8_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP4773086.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
BASE COUNT         5734 a         2453 c         2876 g         5668 t
ORIGIN      
        1 tcgttcaaag agaagttcta ataccaccat atcgtgcgat cgggaacggt atatatatta
       61 ataggagggt aatatatatt taacgcacga tatgggacta ttagccttcg actttgttat
      121 gttgatgtgt ggcctaaaat attggagata ccaatatttt aggttgcatc aacatcaatt
      181 catcttactt cattaaaaca cagcgtgtta tttcatgctt ccgtgtacag tttcaatatt
      241 tgatttcatc attttgtagt aagagtcacc tttagtgcct tctttaccga ttgaatctgt
      301 gtacacttca ccaaagatat ctttcttcgt ttcttcagat aaactttcca ttgctttctt
      361 atcaacactt gtttctacta ataagtgttt taatttgtgc tttttaacaa actcaatagc
      421 ttgtctcatt tgttcaggtg taccttgttt ttcagtgtta atttcccaaa tataacctgg
      481 tgtaataccg tattgttttg agaagtactt gaaggcacct tcacttgtaa tcatggcacg
      541 ttgttctttt ggaatgtcat taaatttgtc tttactgtca ttatttaatt tttccaattg
      601 agcaatgtat ttgttacctt gcttttcata atctgcttta tgttttttgt cgttatcgat
      661 aaatgtttgt tgaattgttt ttacgtattt aataccgtta tctaaactta accatgcgtg
      721 tggatcttgt ttatctttgt tgccttcttc accgtttaaa tagataggtt taacatcttt
      781 tgatactgcg ataacttttt tatcttttaa tgatttacca gcctgttcta aggctttttc
      841 aaaccaaccg ttaccagtct ctaaatttaa tccgttgtat aaaataacgt cagcgtcagt
      901 taacttttta atatctttag gtttaacttc atattcatga ggatcttgac caacaggtac
      961 aatactatga atatcgacgt tgtctccacc aacattttta gccatatcat ataaaattga
     1021 attcgtcgtt actactttta atttgccatt tgacttatca ctgctttgtt taccaccagt
     1081 accacatgca gcaactagaa gtaataaggc taataataaa ggtactaatt ttttcatgtt
     1141 aaacttcctc gtttctttct attcgtaaat tttgtgaaaa ataatgtgat gatataaatt
     1201 acaaacgtac aaagtacgat tgtcgcacca ctaggaatgt tgtaaatata gctgtaataa
     1261 agtccgacaa ttgaacttat gacacttatt aaacttgcta taatcatcat tgagtatagt
     1321 tttttactaa ttaaaaatgc tgtagatgca ggtgtaatta ataatgcaac tacaagaata
     1381 atacctaccg tttgaatact tgctactgtt actaatgaga gtaacaacat cacaaagtaa
     1441 tgtaataacg tcgtatttag accactcatt ctactaaacg ttggatcgaa tgtagaaatc
     1501 attaatggac gatagaaaat aatgattaga ataaggacga ttgaaccaat cacaatagtt
     1561 gttaaaaatg cactatttgt gattgccagt aaattaccaa acagaatatg gtacaaatct
     1621 gtcgtagtgt ttattaagct aataataata atccccgaag ctaagaaagc ggtaaaacta
     1681 attccaatag cggcgtcagg tttcgtttta ctactagatg tgatataacc gataaaaata
     1741 cttgcgatca taccagttat aagtgcgcct acaaacattg gaataccaaa taagaatgat
     1801 agggcaacac caggtaatac tgcgtgactc attgcatctc ccattaatga aagaccacgt
     1861 aatacaatta aactaccaac tgtaccacaa actatcccta caataattga agttatcaat
     1921 gctcgattca agaattgata tgtaaataaa tgttcgacaa actctaacat gttatattgc
     1981 tcctttgact agggtcacta cagtcagtgc tactcataaa tgtttcgttt aagcgagtga
     2041 cactcatagc ctcttcacta tcaccaaagt atcgtaatgt ttgatttaat agaataatgc
     2101 gatcaaagta ttgctttgct tttgatagat catggtggat gataagaata agttttcctt
     2161 gttgttttaa gttctcgatt tttgtcatga ttaatttttc gctactaaaa tcaattccga
     2221 caaacggctc atctagaaaa taaacttcac tttcggacat caatgctctt gctactagca
     2281 cacgttgtaa ttgtccacca cttaattctg aaatttgtcg atgacgtaaa gattctaatt
     2341 ctaaatcgct taataactgt ttgagtttat cccttgctga tttattaggt cgtctaaacc
     2401 atccaatttc tttgtagcaa cctgataaaa tcacttgttc cacacttata ggaaaatcta
     2461 aatcaatatg tgctttttgt ggaatatatg taatatgttg cagttgttgt tgtataggtt
     2521 tgttatataa caatttagta ccggtagcat taaattcacc aattaaagac ttgataaggg
     2581 aagatttacc agcaccattc gggcccatga taccaattat ttcgccgcgt actggtatcg
     2641 ataaggaaat gtttttaagt acatgcttat tacctaaaaa cagatttaaa tcttttgttt
     2701 ctaacaaacg tttatacctc ctaattaaaa gtttaggcta acctaattaa ttgtataata
     2761 aactgagaat atttatcatg tcaagtaaat tcgtgatata atatagacaa tgtatgtgag
     2821 gtgaaagtat gttaactgaa gaaaaagagg actatttaaa ggcaatcctt acgaataatg
     2881 gcgataaaaa ctttgtgaca aataaaatct tatctcaatt tttaaatatt aagcctccat
     2941 ctgtaagtga aatggtagga cgtcttgaaa aagcaggcta tgttgaaaca aaaccataca
     3001 aaggtgttag attaacagag gatggtttaa cgcatacgct tgatatcatt aagagacatc
     3061 gactattaga attattttta atagaaatat tgaaatataa ttgggaagaa gtacatcaag
     3121 aagcagaaat tttagaacat cgaatttcag atttatttgt tgaaaggctg gatagcctgt
     3181 taaatttccc agaaacttgc ccgcacggcg gtgtgattcc tagaaataat gaatataaag
     3241 agaaatatat aacaacgatt ttgaattatg aacctggtga tatcgttaca atcaaacgtg
     3301 tgagagataa gaccgatttg ctaatatatt tgtctagtaa agatatttct attggtaatg
     3361 aagtggaaat tgtatcgaaa gatgaaatga ataaagtaat tatcattaaa cgtaatgata
     3421 atgtaattat tgtcagttac gaaaatgcaa tgaacatgtt tgctgaaaaa taaaataaag
     3481 aagccataaa gatatccatg attgaactga taaagacata tggataattg ctttaggctt
     3541 cttttttatt agttaattta tcaagtgagt atatttgagt aaaatattca ctgcataaag
     3601 attgaagata atccagattg tactataaat gaagataggt acatgactga gttctttaag
     3661 tgcactacca tcccactgtg gactcggacg ctggaaagtc aatttagcaa tcgtccaact
     3721 agattgtaga acttcgccta ataatacacc taaaatatat tgataactca ttgtgacaag
     3781 tagttgaatt tctactatat tttcatcttt taatataaaa tacaacatga tagaaattaa
     3841 agttataaca acaatgggtg agccttttct agatgttaaa attaaaaaat aaataaatat
     3901 caataaatag gtaaatataa agaaactagg tatctgataa tggctcgacg ctaaacctat
     3961 caataacata ataggtggca ttaaataacc accaatcgtt gtaagccatt ggcctgctag
     4021 atgtctagat tgtgtaattg cgaatccttg ttgtaatgtc tgttgtcgct ctcgtggact
     4081 tgttacaatg actaaatctt ttgcacggcc accagcgagt ttattaaaca gtacatgacc
     4141 aaattcatgt gttaaaacag ggatatagtt taaaatgaca tctaaatagt tcaaaacagg
     4201 cttatgtcta tattgatgaa tagcaatata acaagctgca acaataacga taatgtatat
     4261 attaagttga attgtcgtat taaaaaagtt tgataaataa ttcattgtta acctcatata
     4321 agatattaat ttaaagtttg cttagcactt attataaatg atattggcat caatagcgtt
     4381 agactttaga cttaccttag ttaaactaat tttaattttt gaaaaggtga atatgtgtta
     4441 aaataaagca aaatcatttc gatataaata ggatgaatat aaatactgtt aatattgatt
     4501 acactaacat aataatgaaa taagatagga gattcctgtt atgactgttg aagaaagatc
     4561 caatacagcc aaagttgaca ttttaggagt cgattttgat aatacaacaa tgttgcaaat
     4621 ggttgaaaat attaaaacct tttttgcaaa tcaatcaacg aataatcttt ttatagtaac
     4681 agccaaccct gaaatagtga attacgcgac gacacatcaa gcgtatttag agttaataaa
     4741 tcaagcgagc tatattgttg ctgatgggac aggagtagtc aaagcttcgc atcgtttaaa
     4801 gcaacctcta gcgcatcgta tacctggtat tgagttgatg gatgaatgtt tgaaaattgc
     4861 tcatgtaaat catcaaaaag tatttttgct aggggcaact aatgaagttg tagaagcggc
     4921 acaatatgca ttgcaacaaa gatatccaaa catatcgttt gcacatcatc acggttatat
     4981 tgatttagaa gatgagacag tagtgaaacg aattaaactg tttaaacctg attacatatt
     5041 tgtaggtatg ggattcccta aacaagaaga atggattatg acacatgaaa accaatttga
     5101 atctacagtg atgatgggcg taggtggttc tcttgaagta tttgctgggg ctaaaaagag
     5161 agcgccttat atctttagaa aattaaacat tgaatggata tatagagcat taatagattg
     5221 gaaacgtatt ggtagattaa agagtattcc aatatttatg tataaaatag ccaaagcaaa
     5281 aagaaaaata aaaaaggcga aataatcatg atgacaaaaa taaaaccgag gaaatcctta
     5341 aatggagatt ctcggttttt tcggtttatt taataacgaa gcgggactca tcgagtttgt
     5401 ttctaaattc tttttgttcg gctttggatt tctttttaaa atcgttaagg aaagcttcat
     5461 atttaggtaa tacatcatca agttcaccgt aatcttttaa ctttccgcct tcaatccaag
     5521 caatcttagt acaaaattgt ctcacttgtc ctaagttatg actaacgaaa aagatggttt
     5581 tgttttgctc tttaaactcg taaattttat ctaaacattt ttgtgcaaaa gtttggtcac
     5641 ctacagataa agcttcgtca atgactaaga tatctggatt aactgtgata ttaattgaaa
     5701 aaccaagttt tgcacgcata ccacttgaat actttttaac tggttgataa ataaactcac
     5761 caagttcact aaattcaata atcttaggtg tcatcgcttt aatttctttt cgcttaaagc
     5821 ccatacataa cattttaaat tcgatatttt caatccctgt aagttgtcca ctcaagccag
     5881 cactaattgc gataacgctg acttcaccat tacgatccac tttgccaaca gtaggcgaca
     5941 aagaaccgcc aatgatattg ctcaacgttg atttgccgga accattgatg ccaacaagcc
     6001 ctatgacgtc gccttcatat gcttttaaac taatgtcatc taaagcgaaa aatgttttgt
     6061 ttttatgttt gggaatgagc gcatctttca tacgttcttt atttgtacga taaatacgat
     6121 attcttttgt tacattttta atgtttaccg aaacgttcat ttgtagacct tccttattca
     6181 catttatcta gattataata tactactcaa cagttgttaa attttaaaac ctgttgtaaa
     6241 gtgtatagaa gattttgtta ttatcagagt tggtgttttg acacaaaatg ttaatcatca
     6301 atgataacaa tgatatttaa aaactaaact tatttcaact tacatgattg tatactataa
     6361 tgtatttgta ataaactaat attttaaaga actagacaat aattttgata gcatccatgt
     6421 atagtgatag tatttacaac aattattata atactattta gttaagtaga gaaatagtta
     6481 aacatttgaa agtgtggttt aatggaatgt cagcaatagg aacagttttt aaagaacatg
     6541 taaagaactt ttatttaatt caaagactgg ctcagtttca agttaaaatt atcaatcata
     6601 gtaactattt aggtgtggct tgggaattaa ttaaccctgt tatgcaaata atggtttact
     6661 ggatggtttt tggattagga ataagaagta atgcaccaat tcatggtgta ccttttgttt
     6721 attggttatt ggttggtatc agtatgtggt tcttcatcaa ccaaggtatt ttagaaggta
     6781 ctaaagcaat tacacaaaag tttaatcaag tatcgaaaat gaacttcccg ttatcgataa
     6841 taccgacata tattgtgaca agtagatttt atggacattt aggcttactt ttacttgtga
     6901 taattgcatg tatgtttact ggtatttatc catcaataca tatcattcaa ttattgatat
     6961 atgtaccgtt ttgttttttc ttaactgcct cggtgacgtt attaacatca acactcggtg
     7021 tgttagttag agatacacaa atgttaatgc aagcaatatt aagaatatta ttttactttt
     7081 caccaatttt gtggctacca aagaaccatg gtatcagtgg tttaattcat gaaatgatga
     7141 aatataatcc agtttacttt attgctgaat cataccgtgc agcaatttta tatcacgaat
     7201 ggtatttcat ggatcattgg aaattaatgt tatacaattt cggtattgtt gccattttct
     7261 ttgcaattgg tgcgtaccta cacatgaaat atagagatca atttgcagac ttcttgtaat
     7321 atatttatat gacgaaaccc cgctaaccat taataaatgg aagtggggtt catttttgtt
     7381 tataatttaa gtaaataaca tattaagttg gtgtattatg aacgttttaa taaagaaatt
     7441 ttatcatttg gtagttcgaa tactttctaa aatgattacg cctcaagtga ttgataaacc
     7501 gcatatcgta tttatgatga cttttccaga agatattaag cctatcatca aagcattaaa
     7561 taattcgtcg tatcagaaaa ctgttttaac aacaccaaaa caagcgcctt atttatctga
     7621 acttagcgac gatgttgatg tgatagaaat gactaatcga acattggtaa aacaaattaa
     7681 ggctttgaaa agcgcgcaga tgattattat cgataattat tacctattac taggtggata
     7741 taataagact tctaatcaac acattgttca aacgtggcat gcaagtggtg cattaaaaaa
     7801 ctttggatta acagatcatc aagtcgatgt gtctgacaag acaatggttc agcagtaccg
     7861 taaagtttat caagcgacgg atttttactt agtgggttgt gaacaaatgt cacaatgttt
     7921 taaacagtct ttaggtgcaa cagaagagca aatgttgtat tttgggcttc cgagaattaa
     7981 taaatattac acagctgata gagcaacggt taaggcagag ttaaaggata aatatggaat
     8041 tacaaataag ttggcattat atgtaccaac atatagagaa gataaagcag ataatagggc
     8101 tattgataaa gcttattttg aaaaatgttt accaggatat acactgatta ataaattaca
     8161 tccatcaatt gaacattcag acattgatga cgtatcttca atcgacacgt ctacattaat
     8221 gctaatgtca gatataatta ttagcgacta tagttcgctg ccaatagaag ctagcttgtt
     8281 agatattcca actatatttt atgtgtatga tgaaggaaca tatgataaag tgagaggcct
     8341 gaatcaattt tacaaagcaa taccggatag ctacaaagtg tatactgaag aagatttaat
     8401 aatgacgata caagaaaaag aacatctatt aaatccgtta tttaaagatt ggcataagta
     8461 taatactgat aaaagtttac atcagctcac agaatatata gataagatgg tgacaaaatg
     8521 aggcttacga taatcatacc tacatgtaat aatgaggcaa caattcgaca attgttaata
     8581 tctattgaga gtaaagaaca ctatagaatc ctttgtattg atggtggttc tactgatcaa
     8641 acaattccta tgattgaacg gttaccaaga gaactcaagc atatttcatt aatacaatta
     8701 caaaatgctt cgatagctac gtgtattaat aaaggtttga tggatatcaa aatgacagat
     8761 ccacatgata gtgacgcatt tatggtcata aatccaacat caatcgtatt gccaggtaaa
     8821 ttagataggt taactgcagc tttcaaaaat aatgataata ttgatatggt aatagggcag
     8881 cgagcttaca attaccatgg tgaatggaaa ttgaaaagtg ctgatgagtt tattaaagat
     8941 aatcgaatcg ttacattaac ggaacaacca gatttgttat caatgatgtc ttttgacgga
     9001 aagttattca gtgctaaatt tgctgaatta cagtgtgacg aaactttagc taacacatac
     9061 aatcacacaa tacttgtcaa ggcgatgcaa aaagctacgg atatacattt agtttcacag
     9121 atgattgtcg gagataacga tatagataca catgctacaa gtaacgatga agattttaat
     9181 agatatatca cagaaattat gaaaataaga caacgagtca tggaaatgtt actattacct
     9241 gaacaaaggc tattatatag tgatatggtt gatcgtattt tattcaataa ttcattaaaa
     9301 tattatatga acgaacaccc agcagtaacg cacacgacaa ttcaactcgt aaaagactat
     9361 attatgtcta tgcagcattc tgattatgta tcgcaaaaca tgtttgacat tataaataca
     9421 gttgaattta ttggtgagaa ttgggataga gaaatatacg aattgtggcg acaaacatta
     9481 attcaagtgg gcattaatag gccgacttat aaaaaattct tgatacaact taaagggaga
     9541 aagtttgcac atcgaacaaa atcaatgtta aaacgataac gtgtacattg atgaccataa
     9601 actgcaatcc tatgatgtga caatatgagg aggataactt aatgaaacgt gtaataacat
     9661 atggcacata tgacttactt cactatggtc atatcgaatt gcttcgtcgt gcaagagaga
     9721 tgggcgatta tttaatagta gcattatcaa cagatgaatt taatcaaatt aaacataaaa
     9781 aatcttatta tgattatgaa caacgaaaaa tgatgcttga atcaatacgc tatgtcgatt
     9841 tagtcattcc agaaaagggc tggggacaaa aagaagacga tgtcgaaaaa tttgatgtag
     9901 atgtttttgt tatgggacat gactgggaag gtgaattcga cttcttaaag gataaatgtg
     9961 aagtcattta tttaaaacgt acagaaggca tttcgacgac taaaatcaaa caagaattat
    10021 atggtaaaga tgctaaataa attatataga actatcgata ctaaacgata aattaatgta
    10081 ggttattata aaataaatat aaaacggaca agtttcgcag ctttataatg tgcaacttgt
    10141 ccgtttttag tatgttttat tttctttttc taaataaacg attgattatc atatgaacaa
    10201 ttagtgctaa tccagcgaca aggcatgcac caccaatgat agtgaataat ggatgttctt
    10261 cccacatact tttagcaaca gtatttgcct tttgaataat tggctgatga acttctacag
    10321 ttggaggtcc ataatcttta ttaataaatt ctcttggata gtccgcgtgt actttaccat
    10381 cttcgactac aagtttataa tcttttttac taaaatcact tggtaaaaca tcgtaaagat
    10441 cgttttcaac ataatatttc ttaccattta tcctttgctc acctttagac aatattttta
    10501 catatttata ctgatcaaat gagcgttcca ttaatgcatt ccccatcata ttacgttgct
    10561 tctcgccacc aaggttttta tagtctcccg cacccatgat aacttgatta attctaaatt
    10621 tacctcgttt ggtagtaatc gtatggttgt aatttgctgt atcacttgat ccagttttta
    10681 aaccatctgt acccggcaaa ctcatttttg caccttccaa tgaaaagttg aatgtgtaat
    10741 acgtaactgc atgcgttgtt ggtgctaact gctttgtaaa gtctaatatt ttaggtgtct
    10801 ctttaatcac gtgtaaatct aaaatggcat agtctctagc agtcgttaca gtacgttctt
    10861 ggtctttata ctttgttggt gcaaatgaac gtaatcttga attttcagca cccgttggat
    10921 tgacgaaatg tgtatttttc attccgatag ctttagcttt gttattcatt aaatcaacga
    10981 aatcgctggt gttttttgat accttcttag ctaaaattaa tgccgcggca ttactagaat
    11041 tagatactgt aatttgtaat aggtctgcga ttgtccatac ttgtccagga tatagtttcg
    11101 tattactcaa ctcaggtagt gtagacataa tatattcttt gttcgtcatt gtgacagtgt
    11161 catcaagtga aagctgcccc ttatttacag cttccaatgt taagtacatt gtcattaatt
    11221 tggtcataga cgctggattc cacttagtat cgatattgta ttgatacagt aattgtccag
    11281 tttgacttac attaacagca ctcgtcggtt cgtatgcagc tgacaaacct gcataaccat
    11341 attgatttgc tgcttgtaca ggggttacgt cactgttagc agcttgtgca tatggtgtca
    11401 taatacttaa tgttaaacat aaaatgatga taatagatat taaatttttc ataaagcgtt
    11461 aatcttccct tttccaattc ttaaatattc cctaaaagca atggttattc ctacttacgg
    11521 aaatcattgc taattcactt caccttaatt aaattgttga aaataaagtt ttctgcagtt
    11581 aatttgaaaa ataatgcaaa tatattacgt gtgtagctaa aggtgttata atgtttgtac
    11641 gaagagcaaa cttactcaaa agcgattaat tttcatgttt taatataaag actttgagaa
    11701 gttattacaa aaaatgcaat agaaatattc tatcatataa atgttatgag cggtattttg
    11761 gggcaacact ttatttgatt tttaaagttt tgttgggaga aagtatatga tagaaatgca
    11821 tgtatctatc taaatgaatt aactataaat ttcaaacaga agaggtaaaa ctatgaaacg
    11881 agaaaatcca ttgtttttct tatttaaaaa actatcatgg ccagtgggtc ttatcgttgc
    11941 agctatcact atttcatcac tagggagctt aagtggacta ttagtgccac tgtttactgg
    12001 acgaattgta gataaatttt ccgtgagcca tatcaattgg aatctaatcg cattatttgg
    12061 tggtatcttt gttatcaatg ctttattaag cggattaggt ttatatttat taagtaaaat
    12121 tggtgaaaag attatttatg cgatacgctc agttttatgg gagcatatca tacaattaaa
    12181 aatgccattc tttgacaaaa atgaaagtgg tcaattaatg agtcgattaa ctgacgatac
    12241 gaaagtgata aatgaattta tttcacaaaa gctacctaac ttattaccat caatcgttac
    12301 attagttggg tcactaatca tgttatttat tttagattgg aaaatgacat tattaacatt
    12361 tataacgata ccgatattcg ttttaattat gattcctcta ggtcgtatta tgcaaaagat
    12421 atcgacaagt acacaatctg aaattgcaaa cttcagtggt ttgttagggc gtgtcctaac
    12481 tgaaatgcgt cttgttaaaa tatcaaatac agagcgtctt gaattagata atgcacataa
    12541 aaatttgaat gaaatatata aattaggttt aaaacaggct aaaattgcgg cagttgtaca
    12601 accaatttca ggtatagtta tgttgctaac aattgcaatt attttaggtt ttggtgcatt
    12661 agaaattgcg actggtgcaa tcactgcagg tacattaatt gcaatgatat tttatgttat
    12721 tcagttatct atgcctttaa tcaatctttc aacgttagtt acagattata aaaaggcagt
    12781 cggtgcaagt agtagaatat acgaaatcat gcaagaacct attgaaccga cagaagctct
    12841 tgaagattct gaaaatgtat taattgatga cggtgtattg tcatttgaac atgtagactt
    12901 taaatatgat gtgaagaaaa tattagatga tgtgtcgttc caaatcccac aaggtcaagt
    12961 gagtgctttt gtaggtcctt ctgggtctgg taaaagtacg atatttaatc tgatagaacg
    13021 tatgtatgaa attgagtcag gtgatattaa atatggcctt gaaagtgtct ataatatccc
    13081 gttatctaag tggcgacgca aaattggata tgttatgcaa tcaaattcga tgatgagtgg
    13141 tacaattaga gacaatattt tatacggaat taatcgtcat gtttcagatg aagaacttat
    13201 taattatgct aaattagcga actgtcatga ttttatcatg caatttgatg aaggatatga
    13261 cacgcttgta ggtgaacgag gattgaaact gtctggcgga caacgtcaac gtattgatat
    13321 tgctagaagt tttgttaaaa atcctgatat tttgttactt gatgaagcaa cagctaatct
    13381 cgatagtgaa agtgaattga aaattcaaga agctttagaa acattgatgg aaggtagaac
    13441 aacgattgtc attgcgcatc gtttgtctac aattaaaaaa gccggtcaaa ttatattctt
    13501 agacaaagga caggtaacag gtaaaggtac gcattcagaa ctgatggcat cacatgcgaa
    13561 gtataaaaac tttgtagtgt ctcaaaaatt aacagattaa ttttatatat aagtaagctt
    13621 ggagcaaata cacatatacc atcgaggaaa ttaaagtgtg gcacattgat ggatatagat
    13681 gttaataaat tgcttcaagc ttttgtctat tttaaatcat ttgagaagtt acgacataat
    13741 aattcttaaa ttaatgaaat cgatatttta agaaaaaaat gctcatggta taatacaagt
    13801 tataagcaaa catacatata ttaaatactg tagccacgag tcataattct tcatatttta
    13861 catagcaatt taactgattt tagagtccat ggtacagaag tttgatattt caatgtttct
    13921 aaatttttaa aaaattaaat cataggtggg tgccaaatgt ttttattaat caacattatt
    13981 ggtctaattg tatttcttgg tattgcggta ttattttcaa gagatcgcaa aaatatccaa
    14041 tggcaatcaa ttgggatctt agttgtttta aacctgtttt tagcatggtt ctttatttat
    14101 tttgattggg gtcaaaaagc agtaagagga gcagccaatg gtatcgcttg ggtagttcag
    14161 tcagcgcatg ctggtacagg ttttgcattt gcaagtttga caaatgttaa aatgatggat
    14221 atggctgttg cagccttatt cccaatatta ttaatagtgc cattatttga tatcttaatg
    14281 tactttaata ttttaccgaa aattattgga ggtattggtt ggttactagc taaagtaaca
    14341 agacaaccta aattcgagtc attctttggg atagaaatga tgttcttagg aaatactgaa
    14401 gcattagccg tatcaagtga gcaactaaaa cgtatgaatg aaatgcgtgt attaacaatc
    14461 gcaatgatgt caatgagctc tgtatcggga gctattgtag gtgcgtatgt acaaatggta
    14521 ccaggagaac tggtactaac ggcaattcca ctaaatatcg ttaacgcgat tattgtgtca
    14581 tgcttgttga atccagtaag tgttgaagag aaagaagata ttatttacag tcttaaaaac
    14641 aatgaagttg aacgtcaacc attcttctca ttccttggag attctgtatt agcagcaggt
    14701 aaattagtat taatcatcat cgcatttgtt attagttttg tagcgttagc tgatctattt
    14761 gatcgtttta tcaatttgat tacaggaatg atagcaggat ggataggcat aaaaggtagt
    14821 ttcggtttaa accaaatttt aggtgtgttt atgtatccat ttgcgctatt actcggttta
    14881 ccttatgatg aagcgtggtt ggtagcacaa caaatggcta agaaaattgt tacaaatgaa
    14941 tttgttgtta tgggtgaaat ttctaaagat attgcatctt atacaccaca ccatcgtgcg
    15001 gttattacaa cattcttaat ttcatttgca aacttctcaa cgattggtat gattatcggt
    15061 acattgaaag gcattgttga taaaaagaca tcagactttg tatctaaata tgtgcctatg
    15121 atgctattat caggtatcct agtttcatta ttaacagcag ctttcgttgg tttatttgca
    15181 tggtaatatg ttgaagagtg actatgataa tacattttaa ctaataaata tgtccaggca
    15241 tgtcgtctat taatataggt gagatgcttg gactttttta ttattggtat aaagatattt
    15301 aaatattttt aaagttaccg aaattgaagc attataaaaa ccaagtgcac atggtaatac
    15361 acttggcttt tatgggaaat gaatattatt gtacatatga cagtaaggac taggtacagt
    15421 catagtactt cgagcaaaat ttgttttgtt attataaaca acacaaagga gataacttct
    15481 ctattgaaga agttaaaaac attatagcag acaatgaaat gaaagtaaat taaaaattca
    15541 gaatattttt aattattata ttgtgagtga tatttattag ggaaagctat tcttcatata
    15601 aattagttaa atagtaaatc tttgttagaa ctctttccat agagttgtga cagcattttt
    15661 gttgtgctac tatttttata gtctaataaa tatataaagg ggatggtttc gtgaataaaa
    15721 cggttaaaga tttaatacta gttgtcttag gttcatttat ctttgctgca ggtgtaaatg
    15781 catttattat ttctggtaac ttaggtgaag gcggggttac aggtttagca attattttat
    15841 attatgcgtt tcatatttca ccagccatca ctaacttctt ggtcaacgca gtattgattg
    15901 ccataggtta taaatttttg agtaagagaa gtatgtactt aactattctt gtaacaattc
    15961 ttatttcaat atttttgagt ttaacagaat catggcaagt agaaactgga aacagcattg
    16021 tgaatgccat ttttggtggt gtaagcgttg gactaggaat cggagtaatt atccttgcag
    16081 gcggtacaac agcaggtaca acaattttgg cgagaattgc aacgaaatac ctcgatgtaa
    16141 gcacgccata tgctttgctt ttcttcgata tgatcgttgt tgcaatttca cttacagtta
    16201 ttccacttga taaagtatta gtaacagtaa tatcacttta tataggaaca aaagtgatgg
    16261 aatatgtcat agaaggttta aacactaaaa aagctatgac gattatttca actaatcccg
    16321 acaaacttgc caaagcaata gacgagcaaa ttggaagagg tttaaccatt ttaaacggac
    16381 atggctatta tacgcgtgaa gaaaaagatg tcttatacgt tgttatttct aaaacacaag
    16441 tttcaaaagc aaagcgatta attaaacaaa tcgataaaga tgcattcctc gtaattcatg
    16501 atgtaagaga tgtctatggt aatggctttc ttgcagatga ataaataaat ggtatgagca
    16561 cacatactta aatagaagtc cacggacaag tttttgaact atgaagactt atctgtgggc
    16621 gttttttatt ttataaaagt aatatataag acatgacaaa tcgagctatc caatttaaaa
    16681 agtaatgtta gtcaataaga ttgaaaaatg ttataatgat gttcatgata a
//
LOCUS       DBNENW010000047        15767 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNENW010000047 DBNENW010000000
VERSION     DBNENW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.047"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            56..592
                     /gene="amaP"
                     /locus_tag="AC1LW8_001242"
     CDS             56..592
                     /gene="amaP"
                     /locus_tag="AC1LW8_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HIP4773087.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            605..844
                     /locus_tag="AC1LW8_001243"
     CDS             605..844
                     /locus_tag="AC1LW8_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HIP4773088.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            907..1416
                     /locus_tag="AC1LW8_001244"
     CDS             907..1416
                     /locus_tag="AC1LW8_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP4773089.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(1576..3552)
                     /gene="sfaD"
                     /locus_tag="AC1LW8_001245"
                     /gene_synonym="sfnaD"
     CDS             complement(1576..3552)
                     /gene="sfaD"
                     /locus_tag="AC1LW8_001245"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HIP4773090.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLGRTMSLSKDYDTTVHQLFPELIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVTGI
                     HFYNEQDEVDYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSNFESSTKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKVQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PCIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            3654..4847
                     /gene="sfaA"
                     /locus_tag="AC1LW8_001246"
     CDS             3654..4847
                     /gene="sfaA"
                     /locus_tag="AC1LW8_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HIP4773091.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            4834..6591
                     /gene="sfaB"
                     /locus_tag="AC1LW8_001247"
     CDS             4834..6591
                     /gene="sfaB"
                     /locus_tag="AC1LW8_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HIP4773092.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEIVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHAKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            6598..7668
                     /gene="sfaC"
                     /locus_tag="AC1LW8_001248"
     CDS             6598..7668
                     /gene="sfaC"
                     /locus_tag="AC1LW8_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HIP4773093.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(7769..7894)
                     /locus_tag="AC1LW8_001249"
     CDS             complement(7769..7894)
                     /locus_tag="AC1LW8_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773094.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            8060..9043
                     /locus_tag="AC1LW8_001250"
     CDS             8060..9043
                     /locus_tag="AC1LW8_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4773095.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVNPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            9055..10086
                     /locus_tag="AC1LW8_001251"
     CDS             9055..10086
                     /locus_tag="AC1LW8_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP4773096.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            10083..11051
                     /locus_tag="AC1LW8_001252"
     CDS             10083..11051
                     /locus_tag="AC1LW8_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP4773097.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            11287..12657
                     /locus_tag="AC1LW8_001253"
     CDS             11287..12657
                     /locus_tag="AC1LW8_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HIP4773098.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(12789..13049)
                     /locus_tag="AC1LW8_001254"
     CDS             complement(12789..13049)
                     /locus_tag="AC1LW8_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773099.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            13226..13741
                     /locus_tag="AC1LW8_001255"
     CDS             13226..13741
                     /locus_tag="AC1LW8_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIP4773100.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            13785..14972
                     /locus_tag="AC1LW8_001256"
     CDS             13785..14972
                     /locus_tag="AC1LW8_001256"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIP4773101.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            14991..15674
                     /gene="trhA"
                     /locus_tag="AC1LW8_001257"
     CDS             14991..15674
                     /gene="trhA"
                     /locus_tag="AC1LW8_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIP4773102.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT         5304 a         2192 c         2859 g         5412 t
ORIGIN      
        1 ggtacatatt taatatgttt ttaaattaac tttaactaag gaggtgtcgt taggggtgaa
       61 acgacttaaa aactttatcc ttggcttatt aatagtagta atcgtggggt tcctgttatt
      121 catgtatatc caagatggac gtataactga atatcaagac tacttccttc aatttgaatg
      181 gttccaacca ttacttattt cacttgctgc actattgata ttaataggtc ttatattagt
      241 atttagtatc ttcaaaccta cgcatcgaaa acctggatta tacaaagact tcgatgatgg
      301 tcatgtttac gtatctcgta aagctgttga aaagacggca ttcgatacgg ttgcaaaata
      361 tgatcaagta agacaaccaa atgtagttgc gaaactatat aacaaaaaaa ataaatctta
      421 tatcgacatt aaaactgact ttttcgtacc aaataatgtt caagttcaat ctttaactga
      481 agcaattcgt tcggatatta aaaagaatgt agaatatttc acagaaatgc cagtacgtaa
      541 gttagaagtt aatgtgagag atcagaaaac ttctggtcca cgagtgttgt aagggaggta
      601 tatcatggct aacaatcata accaaaacgg acaagactct acacaacaag tgattaattt
      661 tttaaaagta tttaaatgga gaatcgttgg tttcctagcg tttctattaa tcgcgatatt
      721 attcttaacg ttaggttttt ggaaaacagt acttatcatc gttttatgtt taattggtgt
      781 aggtattggg tatatgaaag accgtaagca agattttatg aattttttaa atcgatggag
      841 ttaattcatc aatttagaat ttaattaaaa aaatataaaa attaacaagc aaaaggagaa
      901 tctattatga ctgtagataa caataaagca aaacaagcat acgacaatca aactggtgtt
      961 aacgaaaaag aaagagaaga gcgtcaaaaa caacaagaac aaaatcaaga gcctcaattc
     1021 aaaaataaat taacattctc tgatgaagtt gttgaaaaaa ttgctggtat cgctgcacgt
     1081 gaagttaaag gtatcttaga catgaaaggt ggcttaactg atacattcac taatgcattc
     1141 tcaagtggaa ataacgttac tcaaggtgta tctgttgaag ttggtgaaaa acaagctgct
     1201 gtagacttaa aagtaatttt agaatatggt gaatcagcac ctaaaatctt ccgtaaagta
     1261 actgaattag taaaagaaca agttaaatat attactggtt tagatgttgt tgaagttaac
     1321 atgcaagttg acgatgtaat gactcaaaaa gaatggaaac aaaaacacga aaaaaataat
     1381 gaaaacaata accaagaaag acaaggttta caataagtta ttcgataaaa agctcggcac
     1441 tcaatttaag tgccgagctt ttttgtgatt tttaataatt tgaatatgga aaatgtatta
     1501 ttctctcatt tgtatagatt gtatttaata agttaatgta atccttgagc tcacgattaa
     1561 taaaattcta taaccttaat tattttctcg atacaaaggg ttattaactt taatataagt
     1621 ataatgatgt gcctcatctt caagacgcat tgttgtaaca cacttataat ctatacatgg
     1681 cgcgaacatg gtatctttaa tttcatttat gcgattctca tttacttgat tagatttgtg
     1741 tgtcgaaagt acaagttgat caaaaatgtt atctagtaca tcacgaacga tataccacat
     1801 atgtctttct aagtttgaat catttgatgc tttagagatt gtaagaatta attcgcctaa
     1861 atggttttga acggtagaat aaaaggcttt gttaaacaca gacgtttttg aatcagtaag
     1921 aattcttgat ttttcatgga aatgagatgt actgtatccc atttcattta gttgtacttt
     1981 atcaattctt aaaccttcaa agtctctaat atacatcgtg tccaatagac catctttccg
     2041 aaatgttgca atagcatttt gcaagtgagc ctctaatgca atgccatatt tagtaactag
     2101 tgggattacg agaccaagca atgctttact ataagtttca atccacgatt tcgtcgatga
     2161 ttcaaaatta gacaatgatg cagctgattg ataacgttta atcaatgtca cgataggtga
     2221 ttcattgtta aatgggtagg ttgcaactaa gcttgaagga atcattggtg tgacttcttg
     2281 tggaatcatt tggtatatgt tttttctaaa taatgtgcct aattgttcac ttcgttcagt
     2341 ttgatagtct acttcgtctt gctcattata aaaatgaata ccagtaactt catcaataat
     2401 tgttgatgca taggacttaa atatgacatc tttctccaaa atatcattta aaatacgtgt
     2461 cattagtgga ccattgtgcg tcgtttgttc tgataatgta cgaatttcac ctgtaatatg
     2521 aacgtttgtc gacaatttga tgtgtggcga catagctggg tatttaggaa ctaatgttct
     2581 gaaagataaa ccggcataat aatccaacgt atgttttgct tcaatgatta attctttatc
     2641 tacttctgct tgataatcag aatgtaatac gtcatctaat tgccatggat gaacaatcat
     2701 aatgtgataa tcattaaagt taaactttgg cgtaaattca ttttctaatt gtttaattaa
     2761 ttccggaaat agttgatgaa cagttgtatc ataatcttta gacagtgaca tagtccggcc
     2821 taatttactg tgaatcagta ctattttcaa cttaataggt tgattgaatt ctgaagaata
     2881 taggaatgtt tgtaatgcat ttaaaccttt acgtaattta gccccaggat gtagcggatg
     2941 accttcaata acggcttgct ctgaacgcaa gtaactatct tcgctatttt cgataatatt
     3001 aaataaaggt gcagaatcat gttgcattga cagtgcttga tagctaattg caaatgtcat
     3061 attagttgca ctgtttatta aatcttgctg aaattgatca ctagcagcat tttttaaatc
     3121 tggtgcttca attaaaatac actcaagaat ttcatttgga tggtgtactc gtgtaatcgt
     3181 atttgtaatg tcatctttaa tgtagaaagg gccttcaaca tcaattcgat caaaggcgtg
     3241 ttctccagtg ataggagcat ataatgtttg cttagcttgt gggaactgga tttctagtat
     3301 atgagtcgtt gagatatcta acataatcaa atcacgactc aatattttct tactttgagt
     3361 gcgcgcttta actaagtttt cgcgatgcat tgatgtgact aatctctgag tgactttatc
     3421 tcttccttgt aaaatcatct ctttaaaaat attagcccaa tcgctattat gttgttgtaa
     3481 aaataaatat gtttcttgtt cttctttgtt aaattttaat gtctgttctt taaaaattaa
     3541 gttcaagttc ataattcacc tctatgaaat attttacaaa agcaagatag atttgtataa
     3601 tccatattaa tgataatgat tcttattatc aacagaatgc gggtgtaagt tttatgacaa
     3661 aatatttttt tagcagttct tttctactat ttctaggtaa ttggattgga caaatagggc
     3721 taaattggtt tgtacttacc acttatcata acgcagttta tctggggatt gtcaattttt
     3781 gcagacttgt accaatatta ttactaagtg tgtgggcagg ggcaattgcc gataaatatg
     3841 ataaagggcg attgctgaga attacaattt catcatcatt tttagtaact gcaattttat
     3901 gtgtgctcac gtatagtttc actgcaattc caattagcgt cattattata tatgcgacat
     3961 taagaggaat tttaagtgct gttgaaacac ctttaagaca agcaatctta ccagatttat
     4021 cagataaaat atctactaca caagctgtat catttcattc attcatcatt aatatttgtc
     4081 gttcaatagg gcctgccatt gctggtgtca tattagcagt ctatcatgcg ccaacaacat
     4141 ttcttgcaca ggcaatttgt tattttatcg cagttttatt atgtttacca ttacatttta
     4201 aagtaactaa aatacctgaa gatgcaacaa gatacatgcc gttaaaagtt attatagatt
     4261 acttcaaatt acatatggaa ggtcgacaaa tatttataac atcattattg attatggcga
     4321 caggtttttc atatacgaca cttttaccag ttttgacaaa caaagtattt ccggggaaat
     4381 ctgaaatatt tggtatcgct atgacgatgt gtgccattgg tggtattatt gcaacgctag
     4441 ttttacctag agtacttaaa tatattggta tggtaaatat gtattattta agttcacttt
     4501 tatttggcat tgctttgtta ggtgtggtat ttcacaatat tgtcatcatg ttcatttgta
     4561 ttacattgat tgggttattt agtcaatggg cacgtacgac aaatcgcgtt tattttcaaa
     4621 ataatgttaa agattatgaa cgtggtaaag tactgagtat tattatgatg gatagaggta
     4681 tgattccatt gggaagtctg ttaatgagta tatgtgcaga tgtgtttggc attgttagaa
     4741 ctttttcaat aatgggaata agtactatat gcattacaat ggtattctat tttataaata
     4801 gaaagttgaa gttaaagttg gaggaaagta atcatggtat atcttgaatg ggcaaaggca
     4861 gatagaaata ttcaatatcg tgtaattaac gccattatta aagaacgtat ttaccccgag
     4921 caaacattta tttcgcaaaa aggatcttta attgaaattc agtatcatat gcatgtgttg
     4981 actattgaaa ttgttagaaa aagtgcatta gaacgctacg agtttacagg tgatattact
     5041 tatttaaata aaggtgaaac gtcattaatt ataactttag aaggtttatt agatgtgttg
     5101 aatcatgact ttgatatccc tatttcagag cgactacgcg aagagttaat acacagtcga
     5161 gatagtttag ttgaaacata taagcaaatg tctcacagac aaacgttaat aagtcaaagt
     5221 tttaaatttt caagattacc acaagatatt aacttttttt catggttaca acatgtaaaa
     5281 gatagtgata agacagatga tttaacttat tctgagagtt tggtaccaga ggggcatcca
     5341 acacaccctt taaccaaaac gaaattgccc ttaactatgg aagaagtacg agcatatgca
     5401 cctgagtttg aaaaagaaat ccctttgcaa attatgatga ttgaaaaaga ccatgttgtg
     5461 tgcacagcta tggatggtaa tgatcaattt attattgatg aaataattcc tgaatactac
     5521 aatcagattc gtgtgttttt gaagagttta ggtttgaaaa gtgaagacta tagagcgatt
     5581 ttagtacatc cttggcaata tgatcatacg atagggaaat attttgaagc atggattgct
     5641 aaaaaaatat taattccaac gccgtttaca atactttcaa aagcaacttt atcattcagg
     5701 acgatgtctt taattgataa accatatcat gctaagttgc ccgtcgatgc acaagcaaca
     5761 agtgccgtta gaacagtctc aactgtgact actgtagatg gaccaaagtt aagttatgct
     5821 ttacaaaaca tgttgaatca atatccagga tttaaagttg ctatggaacc gttcggtgaa
     5881 tatgcaaatg ttgataaaga tagggcacgt cagcttgctt gtatcataag acaaaagcct
     5941 gaaattgatg gaaaaggtgc aacagtagtt agtgcaagtc tagttaataa aaatccaata
     6001 gatcaaaaag ttatcgtgga tagttactta gagtggttaa atcaaggaat tactaaagaa
     6061 agtattacga catttattga acgatactct caagcattaa tcccgccttt aattgctttt
     6121 attcaaaatt atggaattgc attagaagca cacatgcaaa atacagtagt gaacttgggg
     6181 ccacattttg acattcaatt tttagtgaga gatttaggtg gttctagaat tgatttagaa
     6241 acattacaac atcgtgtatc agatattaaa attacaaatg atagtttaat agctgattct
     6301 atagatgcag tgattgcaaa attccaacat gctgttattc aaaatcaaat ggcagaatta
     6361 atccatcatt ttaatcagta tgattgtgtt gaagaagccg aattatttaa catagtacag
     6421 caagtagtag cgcatgccat taacccaaca ctaccacatg caaatgagtt aaaagatatt
     6481 ttgtttggac caacaattac tgtcaaagcg ttgttaaata tgagaatgga aaataaagta
     6541 aagcaatatt taaatattga gttagataat ccgataaaaa aagaggtgta gtactacatg
     6601 gcacacgtta acataaatat atcgaagatt aaatataacg ccaaagtact tcaaacagtt
     6661 tttcaaagta aaaatatgca attcacacca gtaattaagt gcatagctgg tgaccgtaca
     6721 attgtagaaa gcttaaaagc gttaggtatc aatcatgttg cagaatccag attggataac
     6781 ataattagta ttgcagatca ggatttaaca tatacgttat tgcgtacgcc tgccaaaaaa
     6841 gagatttcag atatgataga aaaagttgat atgagtatcc aaacagaatt atctaccata
     6901 catcaaataa atgaagtagc agaagtttta ggaaaaaaac ataagatttt attaatggta
     6961 gattggaaag atggccgtga aggtgttctg acttatgatg ttttagatta tattaaagag
     7021 attattcact tgaaaaatat ccattttgta ggtttagcat tcaattttat gtgttttaaa
     7081 tctgatgcac caagtgatga tgatatcttt atgattaatc gattcgtctc agctgttgaa
     7141 agagaaatag gatatcgatt gaaaattatt tcaggtggta attcgagcat gttaccacaa
     7201 cttttatata atgatttggg taaaataaat gaattgcgaa taggtgaaac gttgtttaga
     7261 ggtgttgata caacgacaaa tcaagcgata gctatgttat atcaagatgc gattacattg
     7321 gaggctgaga ttttagaaat taaacctcga gttaatacac aaacgcatga atcattctta
     7381 caagctattg tagatattgg ttatttagat acaaaagtcg ataatatttc acctatggat
     7441 cagcatatca atattttagg cgcttcgagt gatcatttaa tgcttgattt aaatggtcaa
     7501 ggacactatc aagttggaga tcatatatct ttttcattaa attatgaagc actttctcat
     7561 tcgatgtata tgaaaaattt acataaggta tatattgatg attcaaagat tgatacgcta
     7621 cttcaaaatt ttgatgtgaa atctcctgct atggtaaacc aatattaata tatttatcaa
     7681 taagtctaag ttgacaagtt atactacata tcgtttgcac ggttgtatca taattgttca
     7741 acttagattt tttgtatttg ttgatttatc aaattaagtg caacagttcg tcacataaaa
     7801 ttgcaacaga taatatcagc tgaattacag ggataacggt catactaaat ggtgtcaatt
     7861 gtattaatgc aaagatgata tagcaatgat acattatcaa tattttttct aaggaaatgt
     7921 gctaattgta gtcataatta ctagaggaaa tatatagtca tacattttag taatataaaa
     7981 aagattgaac gctacttgac aatgataatt gttatcaata aaataataaa tgaagttata
     8041 catattaagg agtggaacga tgagaggtct aaaaactttt agtatattgg gattaatagt
     8101 tgccttactt ttagttgcag cttgtggtaa tacggataat tcaagtaaaa aagaatcatc
     8161 aactaaagat actatttcgg taaaagatga aaatggtaca gtaaaagtac ctaaagatgc
     8221 aaaacgtatc gttgtattag agtactcatt tgcagatgca ttagcagcat tagacgttaa
     8281 tccagttggt attgctgatg atggtaagaa aaaacgtatc attaaaccag ttagagaaaa
     8341 aattggggat tatacttctg taggtacacg taaacagcca aacttagaag aaattagtaa
     8401 attaaaaccg gatttaatta tcgctgatag cagtagacat aaaggtatta ataaagaatt
     8461 aaacaaaatt gcaccaacat tatcattaaa gagttttgat ggagactaca aacaaaacat
     8521 taattcgttc aaaacaattg ctaaagcttt aaataaagaa aaagaaggcg aaaaacgtct
     8581 tgctgagcat gataaattaa tcaataagta taaagatgaa attaaatttg atagaaatca
     8641 aaaagtgctt ccagcagtag ttgctaaagc tggtttatta gcacatccaa actattcata
     8701 tgttggacaa tttttaaacg aactaggatt taaaaatgca ttaagtgacg atgtaacaaa
     8761 aggtttaagt aaatatttga aaggacctta cttacaatta gacactgaac atttagctga
     8821 tttaaatcca gagcgtatga tcattatgac tgatcacgct aaaaaagatt ctgctgaatt
     8881 caagaaatta caagaagatg caacatggaa aaaattgaat gcagttaaaa ataatcgcgt
     8941 ggatattgtt gaccgtgatg tttgggcaag atctcgtggc ttaatttctt ctgaagaaat
     9001 ggctaaagaa cttgttgaat tatcaaaaaa agaacaaaag taaggtggaa gtaaatggct
     9061 ataaaagaaa taagtagcca atctgccata gatcataaaa gaaaaagacg cacaacactc
     9121 acgtatatag tgagtttgtg ctttcttttt atttgtatat atttaaatat ggcgattggt
     9181 tcttcgaaaa ttaattttag cgatatcgtt cactatgtta ctggtcatac agatacgaaa
     9241 gcaacgtttt tattgcataa tgtacgtatg ccaaggatga ttgcagggtt atttattggc
     9301 ggtgcattag cggtatctgg tttgttaatg caagcaatga cacgcaatcc gctagcatca
     9361 cctaaaattt ttggtgtcag ttctggtgca tcttttttca ttgtatttgt gacgattatc
     9421 attccatcat tagaatatta tgcattatat ttaggtgtga taggtgcatt tataggtggt
     9481 ttaactgttt atacactttc aggtgctacg aaaggtatga cacccattaa attagcattg
     9541 gctggtatgg ccatccattt gttttttagt agtatgaccg aaggtattat tattttgaat
     9601 gagaattcaa acgaacaagt gatgttttgg cttgtcggtt cattatccag tatgaagtgg
     9661 gatgaaattt taaccattct accatggctt attggtgctt taattgtaac gatttttatt
     9721 ggacgtcaac taacgataat ggaactaggc gatgacattg ctaagggatt aggtcaaaat
     9781 ataaataaag ttagaattat cattggacta ttagtcatta ttcttacagg tatgtcggta
     9841 tcagtggcag gaccaattgg ctttgttggc ttaatcgtac cgcatatagt caaacgctat
     9901 gtgagtaaga attatttagt catgattcca ttaacattta ttataggtgc agatttgtta
     9961 ctactatcag atgtattaag tcgtttaatt acatatcctt atgaatcacc agtagggatt
    10021 gttacatcat tcgtaggtgc actttacttc ttatttataa cgattaaagg ggtgaaaaga
    10081 atatgatgac aacgaaaatt atacgtcgtt atagtttaat cacaatcctt ttaattgtga
    10141 gtatattcat aagtttatgt gtagggtctg tcatgattca tccgattgat gctattaagg
    10201 gaatctttac acaagatgat tttattttaa atgagtatcg tatacctaga acattacttg
    10261 gtattattat cggcagtagc ttggctattt caggtgcaat tatacaagct gtaataagaa
    10321 acccgttagc ctcacctgat gttataggta tttctaaagg tgccagttta gctgctgtga
    10381 ttattattat gacctttcca acagcaccat tgtttgtatt acctataggt tcattcttag
    10441 gtgcctttgc agtaagttta tttttatcat ttttaatttc aaaatatgat gttaaaggtt
    10501 caaagcttgc cttaattggt ttagcaatag gcgcaatatg tacggcgatt gtgcagttct
    10561 tattaatcag aaatccactg gaagcaaaca aagcgcttgt ttggttgaca ggtagtctat
    10621 atggacatga tatgtcgaat atttatacta tattaccttg gtttttaata acgataccaa
    10681 tcatcatttt attgagttat caattagatg tattgaatct aggtgatgct gttgcaaccg
    10741 cactcggttt aaaagttaaa actattaaaa tgctattgtt aattttagca gtaatattag
    10801 caggatccgc aatttcagtt gtcggtggta ttagcttttt aggattaatt gcacctcata
    10861 ttgcgagaca attaatagga aataaaacat tacacgttat tatcatgtcg gggttaattg
    10921 gtgctatatt gttaacattt ggagatggtt tagcccgtgg cattcatccg ccactggata
    10981 ttcctgttgg ggttatcatt gcaattatcg gtgcaccata cttcttaata ttattacgaa
    11041 gaatgaaata actaacatat gattagagtt taaaaaaggt taagtacgaa atcagtaatt
    11101 gtacttaacc ttttttgaat tcatcttgaa aaattacaag aaatagcgta tatttaataa
    11161 ctgtaattat tcattatttt gcatacataa acattttgaa agatagaatg ataaggaata
    11221 tgtttgttaa ctgaatagaa ttctgtatat acttaaaacg aatacataga gaaatggatt
    11281 gattttatgg atagcttttc aaggaaagag atagttattg ggagacttaa gtttattacg
    11341 atgtctttaa ttggtatctt attattttta gtaccaattc cagtcgaaca agatggacag
    11401 aagcaaacaa cgttgcccgt ggcattttta gcaggcgtgc ttaaagatgt attaggtggc
    11461 gtaatgccat ttttaattgt gacgataatt actttatcgg gtatcataac tctgatttgt
    11521 tcaactattt taaaagataa attgaaacct gacgggctaa tgaacaatgc ttttaacgtt
    11581 agaattggtt ggttaatttt acgaatatta gcagttgttt ttgcatggat gacattttta
    11641 cgaattggtt caaaggttat ttactcagat gaaacgggtg gattattgtt ctcaagttta
    11701 ttgccaacat tagtggcagt attcttattt gcagctctat ttttaccgct attaatggaa
    11761 tacggtttgt tagagatgtt gggtcctatt tttagaccgg ttatgagacc tttatttaca
    11821 ttgccaggac gttcgacggt agataattta gcatcattta ttggagacgg aactgttggg
    11881 gtcttaatta caagtagaca atatggtgaa ggatattatt ctcgtcgtga ggcaacagtt
    11941 atttcaacga catttagtgt agtttctatc acctttgcaa ttgtagttgc tgaaacagtg
    12001 cacatgcaaa atcaattttt tgctttctat ttatcagtga tagtatcctg cctcgttgca
    12061 gcagtcatta tgccaagaat ttggccttta aataaaattc ctgatgagta tgctaaggag
    12121 gtacctgaaa gtgcgcgtac tgaggcattg ccagaaggaa agacggcatt gagacatggc
    12181 tttgatactg caacagaagt tggtattaaa gcaccgggcg ttattgactt ttttaagtca
    12241 ggattaaaaa cagtcattga tatgtggttt gttattttgc ctgtagtgat gagtattggt
    12301 actattgcta ccattattgc gaactacacg cccttttttg tcatattggg taaacctttt
    12361 gttccatttt tagagttaat gcaaatacct gaagcggcac aggcttctca aacgattatt
    12421 atcggttttg ccgatatgtt tttaccttct atattaattg aaggtgtaca aaatgatata
    12481 actcgttttg ttattggggc acttagtata tcgcagttaa tatacttgtc tgaagtaggt
    12541 ggcgtcatac ttggttcgaa gattcctgta agtatcggca aattgtttat gattttcttg
    12601 attagaacaa tcattacact accaattatt tctttgatgg cacatctttt gttataaata
    12661 tatgtgaaaa aggacaactg ttaaaattat gagaacagtt gtccttttgt atttgttttt
    12721 gctgggtgtt gaatcaatta aaaaaatcat tcatgaagcc ctcaccatga atgatttttt
    12781 tatacaattt atttagttaa ttcattgtaa actttttgat cattttgagt ataaataatg
    12841 tattcgtggt cttcagtatc gataataacg cgattcgttt taccaaacgt tgagccgatt
    12901 ctagatacat ctttgctgtc taatttagga actgcatgaa tatcttggtc ttgtgtaata
    12961 tttttaattt cacttgtagg aattttgata tcagcaactc tccattgaat acgcacttca
    13021 ttattatctt tctttactgt cattgccatt attcaaacac atcctttaaa tattttgtaa
    13081 atacatcata aacgattttg taatcgtttt caagagttat gtgatacttt tttaaattgt
    13141 gtcatactaa tgaattttaa cccgaaaata tgttcgtgtt tttgttaggg atgtggtaaa
    13201 atgtgagtat taaaggagaa caaatatgac aggtaaaaca catgcttcat gcggcatgct
    13261 cgttggagcg ctaacaacac aatattttca aacggatatt ttttcgtcgg ttacggttat
    13321 cattctagca actctagcta gcttattacc cgatatatgt cacactcaaa gtaagattgg
    13381 cagaaggttt aaggtaatta gtttttttgt gagattgatc tttggacatc gaacctttac
    13441 gcattcaata ttatttatcg caatcatagc atttttattg caaattattc agacgcctaa
    13501 ttattatatg gcagcaatta taattgggtt agtgtcacat gttatactag atatgataac
    13561 accaagaggc gtgaaattat tttatccgtt accgtttaat gtgaagacac cgattcaatt
    13621 taaaacaggt ggacttgtag acttatcttt agctacagcg ttaatggtcg gaacagttta
    13681 tgttttattt cagcctttca tcaatgatat aataagcaac tggaatacta agtttttata
    13741 gaaaataaat tattaaaggt tacaaactca gcaggaggca taaaatgcta gataaaaatc
    13801 aattagctaa atataaacaa gatcatttgt gtgaatatga aaaaataatg agtaacaatg
    13861 aaaaagaagc acttgaagag aaggtagcgt cattagattt agattttatt gccaaattat
    13921 ataacgattt atacatcaat aagaaaacaa tagatgatgt atctgcagtt tctgaagtga
    13981 aatatgatat taaatcacaa atgagtgatg atgagattaa gcgtttagag gaacaaggac
    14041 ttcaagctat taaagaagga caatttgcag tacttttgat ggcaggtggt caaggaacaa
    14101 gacttggtta caagggccct aaaggttcgt ttgaaattga aggtgtaagt ttatttgaac
    14161 ttcaagctaa ccaattaaaa acattgaatc atcaatctgg acatacgatt caatggtata
    14221 ttatgacaag tgatatcaat catgaagaaa ctttagctta tttcgaagca catagttatt
    14281 ttggatatga tcaagaagca atccatttct ttaaacaaga taatattgtg gcgcttagtg
    14341 aagaagggaa gttaatttta aatcaacagg gtcgtattat ggaaacgccg aatggtaatg
    14401 gtggcgtgtt taaatcttta gacaaggcag gatacttaga agaaatgtct aataatggcg
    14461 ttaagtatat tttcttgaat aatatcgaca atgttttagt aaaagtgttg gatccactat
    14521 ttgcaggatt tacggtagaa catgattacg atattacttc taaaacgatt caaccaaaac
    14581 caggagaaag tgttggacgt ttagttaatg tcgattgtaa agatacagta ctggagtatt
    14641 cagaattaga tccagaagtt gcaaatcaat ttaacaatgc taatatcggt attcatgcat
    14701 ttaaacttgg ttttatttta aacgctgtaa atcgtgagtt accttatcat ttagctatta
    14761 agaatttgaa acaattagat gagaactttg gggttattga acaaccaact ttaaaattcg
    14821 aattgttcta ttttgacata tttacttatg gaactagttt tgtcacgtta caagttccta
    14881 gagaagaaga attttcacct cttaaaaata aagaaggtaa agatagtgtt gcaactgcaa
    14941 cagaagattt acgtagaatg ggtttaattt aatgataggc ggtgactatg atgtccaaat
    15001 caactaaaaa aagtaatgac acaatcgtag aaacatttaa ggatatcatc cctttatcat
    15061 ttggagaaga aattggtaat gcggcatctc atggtgttgc agctttgtta acattattag
    15121 tgttacccta tgcggctgtg cacagttata ttgcacatgg tacgcttgcg tctgtgagta
    15181 tgtctattta tgttatttct atctttatga tgtttatttc atctactatt tatcattcta
    15241 tgcaaaatga aacacctcat aaatatattt taaggattat cgaccacagt atgatttacg
    15301 tagcaatttc tggtacatat acgcctattt tgttaacagt tgtaggtggt tggataggtt
    15361 ggacagtatt tattttatta tggggaacaa cactttgggg tattttatat aaatccatag
    15421 ctgtcaaagt taaccaaaag ttaagcttaa ttgtctattt agtgatgggc tgggtaggta
    15481 ttatcttgtt accaattatt tttattcgca catcatggat attcatatta ttcattttct
    15541 taggtggtgt gtcttataca attggtgcat ggttttatgc acaaaagaat agaccatatt
    15601 ttcatatgat ttggcatatt tttattgtaa tagcatcact attgcattta attgcaatct
    15661 tatattttat gtagtcgttt agaaaagctg ggggaaactc cgcttttttt attacataaa
    15721 tcattctaat aggggattgt attgtaatag aattatcgtt aagattt
//
LOCUS       DBNENW010000048        15740 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNENW010000048 DBNENW010000000
VERSION     DBNENW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.048"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(134..649)
                     /locus_tag="AC1LW8_001258"
     CDS             complement(134..649)
                     /locus_tag="AC1LW8_001258"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIP4773103.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(780..941)
                     /locus_tag="AC1LW8_001259"
     CDS             complement(780..941)
                     /locus_tag="AC1LW8_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1561 family protein"
                     /protein_id="HIP4773104.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            1165..2316
                     /gene="yfkAB"
                     /locus_tag="AC1LW8_001260"
     CDS             1165..2316
                     /gene="yfkAB"
                     /locus_tag="AC1LW8_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HIP4773105.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(2576..3106)
                     /locus_tag="AC1LW8_001261"
     CDS             complement(2576..3106)
                     /locus_tag="AC1LW8_001261"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIP4773106.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3195..4451)
                     /locus_tag="AC1LW8_001262"
     CDS             complement(3195..4451)
                     /locus_tag="AC1LW8_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIP4773107.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(4454..4654)
                     /locus_tag="AC1LW8_001263"
     CDS             complement(4454..4654)
                     /locus_tag="AC1LW8_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HIP4773108.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            4801..5265
                     /locus_tag="AC1LW8_001264"
     CDS             4801..5265
                     /locus_tag="AC1LW8_001264"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIP4773109.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            5272..5547
                     /locus_tag="AC1LW8_001265"
     CDS             5272..5547
                     /locus_tag="AC1LW8_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIP4773110.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            5825..7036
                     /locus_tag="AC1LW8_001266"
     CDS             5825..7036
                     /locus_tag="AC1LW8_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIP4773111.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKNKSNPKIDVDRTYIEPQQFQS
                     KKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPM
                     LLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIW
                     SASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISH
                     FLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFTSI
                     IWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSV
                     IKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDKNI
                     KDKIVDKLKKD"
     gene            complement(7148..7777)
                     /gene="vraR"
                     /locus_tag="AC1LW8_001267"
     CDS             complement(7148..7777)
                     /gene="vraR"
                     /locus_tag="AC1LW8_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VraR"
                     /protein_id="HIP4773112.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(7767..8810)
                     /gene="vraS"
                     /locus_tag="AC1LW8_001268"
     CDS             complement(7767..8810)
                     /gene="vraS"
                     /locus_tag="AC1LW8_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VraS"
                     /protein_id="HIP4773113.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(8807..9508)
                     /gene="vraT"
                     /locus_tag="AC1LW8_001269"
     CDS             complement(8807..9508)
                     /gene="vraT"
                     /locus_tag="AC1LW8_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     VraT"
                     /protein_id="HIP4773114.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(9523..9909)
                     /locus_tag="AC1LW8_001270"
     CDS             complement(9523..9909)
                     /locus_tag="AC1LW8_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773115.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(10126..10884)
                     /gene="map"
                     /locus_tag="AC1LW8_001271"
     CDS             complement(10126..10884)
                     /gene="map"
                     /locus_tag="AC1LW8_001271"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIP4773116.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            11415..12401
                     /locus_tag="AC1LW8_001272"
     CDS             11415..12401
                     /locus_tag="AC1LW8_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIP4773117.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(12891..13304)
                     /locus_tag="AC1LW8_001273"
     CDS             complement(12891..13304)
                     /locus_tag="AC1LW8_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773118.1"
                     /translation="MKPTKLILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(13333..13444)
                     /locus_tag="AC1LW8_001274"
                     /pseudo
     CDS             complement(13333..13444)
                     /locus_tag="AC1LW8_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13530..14261)
                     /locus_tag="AC1LW8_001275"
     CDS             complement(13530..14261)
                     /locus_tag="AC1LW8_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIP4773119.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(14263..15576)
                     /gene="murT"
                     /locus_tag="AC1LW8_001276"
     CDS             complement(14263..15576)
                     /gene="murT"
                     /locus_tag="AC1LW8_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HIP4773120.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
BASE COUNT         5067 a         2802 c         2275 g         5596 t
ORIGIN      
        1 aaaaataaaa gaccatccct attaaatgcc aatagagacg accttttatt tgttattcat
       61 tttttaaaac taaaatccat atttcatttc aaacgaaaat atataaattt taacaattga
      121 taaccgcaat actctattgt aattgcttaa cgatttctcg attaaaatca tctaaatcgt
      181 ctggtactcg acttgttaca atattgttgt ctacaactac tgactcatca actacatgtg
      241 cgcctgcatt tgataaatct ttgcgtacat ttaatactgc tgttaacgta cgacctttta
      301 aatcgtctgt atctattagt atttgtggcc catgacaaat ggcaaatgtt ggtacatcat
      361 ttttagtaaa gtatttagca aatgtgccat atcgaccttc tgtatctcca cgtaaatgat
      421 ctggtgaaaa tcctccagga attaataatg catcataatc ttctggttta gcttctgcaa
      481 tgcctacatc gacagtaact ttttcaccgt gtttaccaac aacttcacta tttgcagtat
      541 ctccaatcac tacagtatta aagcctgcat tctctaatgc ctctttaggg cttgaatatt
      601 ctatatcttc aaattcgttt gctagaataa ttgctacttt tttagtcatt gaaaatcacc
      661 tttctatata tcattgatat aattactata gacaagtaaa tcagtgatta aacatacaag
      721 atataaaaat tattaagcga ctgtcgcgat atctaaccct aacacatctt atgtggcatt
      781 tacttagata ctaatttaac cttttcttca agctgatcta acaatccaat ccattcatct
      841 atatcttcaa cacgtacttc atcaggattt acatgatcga tatcctcaat aaacttattt
      901 aaacgcgctt ttatctgttc gattgtttgc tgttcattca taaaaagtta actcctttta
      961 ttttgttttc tttttcatta ttatcctaac agaaattgcg ttaaagcgat ataatcttag
     1021 ctatatttat gacattcaaa ttattttgac ttttaaaaat ccccttttca attaactaaa
     1081 attaagagat aatttgttac gagtgataat acgaagtggt atcataccga tatgaaccaa
     1141 atagaaagaa ggaagtttaa gacgatgaat agcgtcaaat tgaagcaacc tgttagcatt
     1201 tacaatgatc catgggaatc atataacgat gttaaagaac atggccaatt aactttaagt
     1261 aacatcgaat ttacaactac aaatctttgt aatatgcgtt gtagccactg tgcagttggt
     1321 tatactttac aaactgtcga ccccgagcct ttagatatgg acttaattta tcgtagactt
     1381 gatgaaattc caaatctgcg aacgatgtca attacaggtg gcgaaccaat gttttctaaa
     1441 aagtctatta gaaatgttgt taaacctcta ttaaagtatg cacatcatcg aggtatatat
     1501 acacaaatga attcaaacct aacattgcct caagatcgtt atttagatat tgctgaatat
     1561 atcgatgtta tgcatatctc acataactgg ggaacaactg atgaattcgc aaatgttggc
     1621 tttggcgcaa tgaagaagca accaccgtta aaagctaagt taaaattata tgaacaaatg
     1681 atttcgaatg cacgtacatt atcagaacaa ggaatgtttg tatctgcgga aacaatgctc
     1741 aatcaaagta cgctaccaca tttacgaaaa atacatcaag aagtcgttca tgatatgaaa
     1801 tgtagcagac acgagattca ccctatgtat ccagctgact ttgcaagtca attaaatgtg
     1861 ttaactctag cggaaatgaa aaagacaatt catgatatat tggatttcag agatgaagat
     1921 atttggatgt tatttggtac tttgcctgtg tttccatgct taaaagatga tgaagatcaa
     1981 aagttactat cacgtttaag aactgctaaa aatgtaacga ctagaaatga cccggatggc
     2041 cgtagtcgtt taaatgtcaa tgtatttaca ggtaatgtaa tcgtaactga tttcggagat
     2101 gaaacaggta caatttcgaa tatacaaaaa gataaattaa cagatgtatt tgataaatgg
     2161 ttatcctctg atcttgctaa atcattaaat tgtcattgtt ccgagtttag ttgtttagga
     2221 ccaaatgttc ttgttaaaaa tatgtactat ccgaatatgg attttaaaga taatgagcgt
     2281 catatgcaca aacaaccaca aattatacaa ttttaaaaac tcttaattat gcggagaagc
     2341 actttatcga taagtagtct ccgcatattt taatgctatt ataaaataaa aaacaattaa
     2401 ttgctggcag tactctactt taataataaa ggtcatttaa taggactaat agtctataat
     2461 aaaagggtta aattttaact aaaagcataa acgtgcataa tcaaaaagac agattgtagg
     2521 tggaatattc gaacataaca gttcaattca tccttaacaa tctgtcttta tatttttagt
     2581 ctctaatatg ttgcacttga gctaaatatt caattgtttg tttactttca atgcgacgtg
     2641 cttttcttcg ttcaacacgt tgtggtgctg tatcataaaa ccatttttca acatcatctt
     2701 ctggatatac accaggtaca tgtttaggtt tgccttcatc atctaacgca acaaatgtta
     2761 ggtaacttaa tgcagctaaa tcatgcttgt tattaaatac atcgtcaatt ctaatttgaa
     2821 cgaccacttc cattgaacta gtcccagcgt atgaaaccat cgctacgtat tgtaatatgt
     2881 cccctgtttt aatcggcttt aagaaatcta ctgagtctgt agatgcggta actacttgcg
     2941 caccagcatg tttcatagct gtgattgctg caatttcatc aatattagcc atcaatgtac
     3001 caccaaacat tgtatgatgg tgattcgtat cttgagggaa aacttgtcta tttttataac
     3061 attttgattc tgacatagat ttcattggtc tgtcctgatt tgtcattatt ttatctccca
     3121 atctatactt taatcacgta tttgaccttt aaatacatgt ttcatttcct actcagcacc
     3181 tcaaaataca tttattaaaa tgttgatgcc caatttccat tttcaaatac tagttctttt
     3241 gaaccatctt cgaatatgcc ataaatagtc aaatcactac taccaatcat gaaatcgaca
     3301 tgtacgtttg aatcatttaa tccgcttgca atcttttcct caacagtcat ttccgttcca
     3361 ccttgaatat taaaggcgta agcagatcca atcgctaaat gacatgctgc attttcatca
     3421 aataaagtat tgtaaaaaat ggtattacga tttgatatcg gtgaatcatc aggtactaat
     3481 gctacttcac ctaatcttct tgaaccttca tcagtattga ttaaatcttt caataccgct
     3541 tcaccttttt cagctgaaaa atcaataatc tctccatctt taaacatcaa tttaaattga
     3601 tcgataattg tgccattata acttaaaggc aatttattag taacatatcc atctactcta
     3661 tttcgatctg gtgctgtaaa tacttcttca gttggtatat tagcaataaa agcttgttcc
     3721 ttgccgttaa cataacttgt agcatcttcc cagatatgat ttttcgctaa acctactgtt
     3781 aaatcagtac cttcagatac ataatgcaac gcatggtaat ttttttgttg taacttttga
     3841 gcgtaaacac ttaaattagc gatgtgttga cgccaatttt caactggatc gttaccatca
     3901 attcgcacaa tatcgaatac ctcgtcaata aacttaatat atgcttcttc cacagatagt
     3961 tcaggataca ctctcttcgc ccaagcttta gatggaaaag cagctacgac ccatggaaat
     4021 tgattttttt gtacactttc catatatcct ttaaatgcgc gtgcgttttg ttgttgaaat
     4081 gcttgtaatt tttggctatc gataccatcc attaaatcag gatcttcact aataagtgct
     4141 aaattagcag cgccacgttt aacgtaatcc attctcgctt caacatcata tgattttatc
     4201 tcatgattag caaaatgttc cacagattca ttttcaaatt ttaagcgttt taaggttgga
     4261 tcgctatata caacacgtac atctgaagcg ccacaatgat atgcttcttc tacaattaaa
     4321 tgcgttaatt ctaatgtttc tactgatgat ctaataaata ctggctgttt tggttgaaca
     4381 ttcataccaa ccttaactaa tagttcagcg tattgttgta acttttcttt gtaattagtc
     4441 aaaattccca ctcctagtcg tgtcgcaatt gtcctaaagc gtcagcaata gctgtcactt
     4501 cacttggcga caatctttct cttgattgaa caaaatcata tatatctgtt aattcttctt
     4561 catttgcatt tttatatttt tcaggatcta ataatccttg attaacaata tttaacttct
     4621 ctctaatagc taatactttc tcttcatttg tcatagccac aatgttcacc tctaataata
     4681 gttatgttat caaagtatca caatttaata aactttaata gaattttata taatctgctt
     4741 tcttattgtt taaagtcgct ataaaatggg gatataacta catagtttgg aggaatcaaa
     4801 atggtagatg tagcatttgt ctgtcttggc aatatatgtc gttctccaat ggcagaagca
     4861 atcatgcgac aaagacttaa agacagaaat attcatgata ttaaagtaca ttcaagaggt
     4921 actggtagct ggaatttagg agagccacct catgaaggta cacaaaaaat tctcaacaaa
     4981 cacaatattc catttgatgg catgattagt gaattattcg aagcgacaga tgattttgat
     5041 tacattgtgg ctatggatca aagtaacgtt gataatatta aatctatcaa tcctaatctt
     5101 aagggacaat tgttcaaact gttagaattt agtaatatgg aagagagtga tgtaccagat
     5161 ccatactaca cgaataattt tgaaggtgta tacgacatgg tattatcatc ttgtgataat
     5221 ttaatagact acatcgtaaa agatgcaaat ttgaaagagg ggtagttttt tatggaaaat
     5281 aaatttgttc ctggtatttt aattggtgcc gtaattggtg gtgcaattag tttagctgat
     5341 aaatctacac gtcaagcttt agttcaatca gttaaagatg caaaaaatgg taaccgcact
     5401 cgtaagcctt ctaaagtcag caagattaaa gacgaagttt tatactggaa agatgttgtt
     5461 gaagaaattc gtcgtaataa tcctgaatta gaacgttcat taaaagatgc gaaagaaaca
     5521 tttgttaata gaaaaaatca acgctaagat gattttgttt ttataaggca atacttaaca
     5581 atttggtgta cgcctttcat agttatcatt attaaatgat aatacaatga ttggccaaac
     5641 tgcattgttt cagtattgcc tttgttttaa ttttaaatca aaatagccta tgaaagattt
     5701 aaatcaatta atttctataa tattatcatt tttaaagcat atcattgttt agttttttta
     5761 taattggata aatactaata gttactttat aaaacattac atagagaaag gttaaggagt
     5821 gcacatgtcg aaaaaggatc actcttcttc aaaatatctt aattctgtta aggaagcaca
     5881 agaggagtca aaaaataaaa gtaatcccaa aattgatgtt gatcgtacat atattgaacc
     5941 tcaacaattc caatctaaga aacctaaaaa agatgatcag gttttcttct tatcaagatt
     6001 aaataaacct gcaaaatata agaaagactc taatttctta tcatatctca tctatcgcat
     6061 aggaaaagat gatgcctcag gactagcagc acaaatgact taccatttcg tacttgctat
     6121 gttccctatg ttgcttttcc tattaacatt attaccattt ttcaatatta agcagagtca
     6181 aattactaat atgttaagca atgcacccgc tgaaacatct actctaatta agagtgtaat
     6241 tggtgatata actcaaaact ccagtggtgg cttattatct atcggtttga ttttagcaat
     6301 ttggtcagct tcaaatggaa tgactgcaat tatgaattct ttcaatgttg cttacgatgt
     6361 agaagatagc cgtaatggaa tcgtattaaa actactaagt gttgtcttca ctgtagttat
     6421 gggcgttgtg tttgtagttg ctctagcatt accaacgctt ggttctgtaa ttagtcattt
     6481 cctattcggt ccacttggat ttgacgaaca agtgaaatgg atttttaacc ttattagaat
     6541 tgtgttacca atcattatta tatttatcgt atttatcgtg ttatattcgg ttgcacctaa
     6601 cgttaaaacg aagcttaagt cagtattacc aggtgcagta tttacttcaa ttatttggtt
     6661 agctggttca tttggttttg gttggtatat ttcaaatttt ggtaactatt ctaaaacata
     6721 tggcagtatc gcgggtatca tcattttgtt actatggtta tatatcacaa gttttattat
     6781 aattgtcggt gctgaaatca atgcaatcat tcatcagcgt agtgtaatta aaggtaaaac
     6841 acctgaagaa gcagcattag aacatgatga caataataaa aatcattata atgagaacac
     6901 tagatacgaa tatgatgaag atagtaatgc aacacatcaa cgtacgtacc atgttgatga
     6961 acatcctagt gatacctatc cagaagatga taaaaatata aaagacaaaa tcgttgataa
     7021 gcttaaaaaa gactaaacac caacaaaaca gaggttttcg ctaatgattg cgaagacctc
     7081 tgtttttggt gtctatttga tgttatattg tgattggcgt aagtaacttt tcttaattca
     7141 atacgaacta ttgaattaaa ttatgttgga atgcatagat gacagcttgt gttctatctt
     7201 gcacttctaa cttacttaaa atgttactca catgtgtctt aaccgtttta atagtaatat
     7261 gcgatgcact agcaatttct tgatttgagt aacctttcgc aatcaataat aatatttcca
     7321 tttctcgttc tgtaagcatt tcatataact ctgcgcgctt tttcatacgg ttacgcattt
     7381 tcactaaaac ttccggttca aaaacagatt ctcctctaga agttttacga actgcatcgg
     7441 cgatatcttt tgcacttgtt gtttttaaaa tgtaactatc gacacctgca tctaatgcac
     7501 gatatacctc tttatcttca ataaaactag ttaacattaa tactttaatt tgcggtaaat
     7561 cttttttaat ctgagtcgtc gcttctacac catccatgtc ttccataagt aaatccatta
     7621 aaattaaatc tggcttcaac tcatgggctt tggcaattgc ttccttacca gaagcgcctt
     7681 caccaactac ttcaatatca ctttgcgttg atagataact tgaaattcct atacgtacca
     7741 tttcatgatc atccacaaac aatactttaa tcgtcatacg aatcctcctt atttaaaggt
     7801 gctttcacct cgatacgtgt acctgaatct ggcaatgata caatatggaa cgttgcacca
     7861 atttccaaag ctctttcacg catattttta agtccataac tttgttctaa tttttcatca
     7921 acattaaaac ctttaccatt gtcttgaatt ctcaacaata aataatcgtc tttattaaac
     7981 aattctactg tcacttttgt accgtttgaa tgacgcaatg tattcgaaat tgcttcctgt
     8041 gtaattctga acaaatgatc ttcaatacct ttaggcactt taaaatcttg tatttcatgc
     8101 acaactttca tcggcacttt tttttgtaaa tcaataacta aatctttaat accctcacct
     8161 aaagatttgt cttttaaacc aagcggtctt aaatgtaaca gcaaagcacg catttctaac
     8221 tgcgaatctt gaaccatttt ctctaaaatc ggaatttgtt ggtctaatgg tggttctaac
     8281 ttcgtttctt tgatagcaga aagcatcata cttgccgcaa aaagttgctg actaacagaa
     8341 tcgtgaagtt ctcgagctag tctttgacgt tcatcttcaa taatcttttt aactttcaca
     8401 tcattaatat tataattttc attggttaaa ttttgagttt taagtcgcaa cttatgcaat
     8461 tcttgattta aaggtacgag tgtatggtat aaatctaacg tttcactata tatttctata
     8521 ttttgatcat taatgccaac tgtttcgcct tccattgaac gctcaatttg cgtcttaatc
     8581 caatcatttt gctgattgat tttgtatgcg agtaccgaac caacaataat acacaataat
     8641 atgatgatga gatttaaaaa taaaaagact ggtattccga atatttgtgt ataaaacata
     8701 ccttgaaaat agatgatatt tacaaaaact ttatcgatga atagaaatgc agctagcatg
     8761 ctatatacta agatgagcat tgaaccaatt gttctaatgt agtggttcat cgataaatca
     8821 cctctacgtc tccgataaac gttgatacgt agatattaac tgtatagtta tccggtttca
     8881 tcatttcttc aatatgaata ttgttatttt caactttata tgatttttca ttcacgtaag
     8941 tacttccata aaaagcagct acatgtaaat taatattgta attaaccggc aatataacct
     9001 gcactttacc taaaatgtgt ctaacaacaa tggtattatt ttccttaata tttgcagctt
     9061 ttgttaagtc aatatgtagg tcgccaattc catgttgaat ttggacatct tcccatttat
     9121 atacataaac tggtgtacgt tgctcaccaa accacttttg tttaataaaa gatggtgaag
     9181 tcataacctc atccgtcgca actacttttt ttggtttaaa tttgtgtatt aaataacgca
     9241 caataagtag taataaaaag acgaacaaga tgatgattgt atacttatta gacaataatg
     9301 tgaatgcaat taatagcgcg cctatccaaa acgccaaaag gccacgtatt ttatgaaaat
     9361 aaagatatcc tacataaact aatacacatc ccaataatag aacgagtaaa aagccaattt
     9421 tttcaaaaaa tatgtagtaa aaattggcaa taatcattaa tgcagtaaaa atgatcaaca
     9481 tttgcgttga tatatatttg tgtgtcatgt ttcgccgcct ttctatgcca cagcgttcat
     9541 ataatttatt agatacgatg ttttttctgt tgctattttt tcagttgaag tttgttgaat
     9601 ttgagtttca atttgtgcat aatatgcttc aactttattc aactctgatt taatattttc
     9661 aatttcttga ttattgattt ctcttgcaca ttgaatttga aatgcttcag caatatcaat
     9721 atatttattt tctaatgtta gcattagact gtcaattaac ttgctaagtt gttctttttt
     9781 agtaattaaa taacgcatat aatcatctaa atttttcatt ttttcatcta gcattaaaag
     9841 gtagtgcttg atattatttc ttactgttgc tctcaaaaac tgttcaatat aacgttcaac
     9901 atagttcata actatcacct tttataataa gttttaaaat accaaatgcg ctatgtatct
     9961 tcgttgatgt cgatgatatg tttgcttaaa aactacttta gtacaacata tttttgaaat
    10021 gacgcattga ttgtgttctt aattattata aaacagttcc gaaaccaata tgaataggct
    10081 ccggaactaa atgatgttca tactttagtc ttagtatatg ttgaactatt cttcttcaat
    10141 ttttgtcgtt aaaatcggac catccttagt cacgataacc gtatgctcaa tttgagcaac
    10201 aaaactttta tcgctcgttt caaaagccca ttcattttta ccttctgtaa caaatgatgc
    10261 atttgatgag ataaacggtt caatagctaa taccatacct tcagttaata atgttttgtc
    10321 ttttggatca aagtaattaa gtacatgtgc tggtgcttca tgtaatgata aaccaacacc
    10381 atgacctgtt aagtttttaa tgactttcaa atcattttgt ctagctgtat tatgcaccgc
    10441 tttaccaatg ttacttaact tagtacccgg ttttactttt gcaattgcat tctcaaatgc
    10501 catcgttgct acgtcacata ctttttgttt cattggatca tctgattctc caacgacaaa
    10561 tgaaatgcct gtatctgcat aatagccatt cttcaaagcc gatacatcaa tatttactaa
    10621 atctccttca cgaatgacac gcttacttgg aatcccatgt gccacctctt cattgacact
    10681 aatacacgtt tgaccaggaa aattttcatc atgaattggc gcagaaatag caccgtattc
    10741 ttcaaataac tctttcgcaa tattatcaag ctctttcgta gtgatacctg gtttggttgc
    10801 agcttgcatt gtattgcgca ctttagcgca tatgtatcca atttctttta acgcttgtaa
    10861 ttcttcttct gtttttacaa tcattttatc ccgttccttt tttatataat tataacttat
    10921 tatagcaaaa attctttgat atactaaaga tgttacttta gtattttgga aagtgagtga
    10981 gacatagtgt gataaagaaa aaatatttct ttatcgttgt accccaactt gcattgcctg
    11041 tagaaattgt agatccaatt tctctatgtt ggggccccgc caacctgcac attatcgtaa
    11101 gctgactttt cgtcagcttc tgtgttgggg cccacacccc aacttgcatt gcctgtagaa
    11161 tttctttttt gaaattctct atgttggggc cccggactat aattgaaaaa tgcttgttac
    11221 aagtgcattt tatttcagtc aactactaac aatataacat tgtggagccc agaactttga
    11281 ttaatgtata tgaaaatcaa agtaatgctt aagtatgatt atttcaaata ttttacatac
    11341 atgaactttt ccaatgtacg atactattat tataaagcgc tcgctaagat tttacgatga
    11401 ttagaggtta aaaaatgaac gatcaatggt ataaacattt aattggtgcc agaacaatta
    11461 aaactggtat tgccattttt ttaacagctg tcttttgtat ggcactagat ttaacaccca
    11521 tctatgccat tttaacagct gtagtcacaa ttgaaccaac tgccaaggca tcacttatta
    11581 aaggttatcg tagattacct gctacggtaa ttggtgcagg ctttgcagta ttatttacat
    11641 atttattcgg cgatcaatca ccttttacat atgcattgag tgcaacgttc acgattttat
    11701 tctgtacaaa actcaaattg caagttggta cgaatgtcgc tgtactaaca tcattagcta
    11761 tgattccagg tattcatgat gcctacatat tcaacttttt gtctcgaaca ttgacagcaa
    11821 ttattggact cgtgacatca ggtttaatta acttcatggt gtttccacct aagtattatg
    11881 gtcaggtcga agaaaaatta agtaagacag atgccttgat gtataaatta ttttacaatc
    11941 gctgccaaga acttatctta tcaagactgc aatctgataa aagcgaaaaa gcatataaaa
    12001 atattttcaa tttaaacaat caagttgaaa cgttaataag ttatcaacgt gatgaattaa
    12061 gctatcataa gaaaaaagaa tgtgattgga aattactcaa tcaattaact aaacgtgcat
    12121 atacgaatcg tttgtttatc actcatttat caaatatcat ttacttacct aaaaatactc
    12181 gtgttaactt ttcaggtgac gaaaaaatgg cattgctaaa aattagtagt agcatcaaag
    12241 atatatttta tgatggttct tttaagagag aagatgattc tgtagaaact ttacgttcga
    12301 caataaaagc acttgaaatt agtggtgaaa atcaaattaa aagtcatata ctatacgaag
    12361 ttctaatgat ttatcgactg ctagacagca gatatgctta atttaagtac cttatactaa
    12421 ttggcaccat gggagtggga cagaaatgat attttcgtaa aatttatttc gtcgtcccac
    12481 cccaactcgc attgcctgta gaatttcttt tcgaaattct ctgtgttggg gcccacaccc
    12541 caacttgcat tgtctgttga aattgggaat ccaatttctc tttgttgggg cccacacccc
    12601 aacttgcatt gtctgtagaa attgggaatc caatttctct ttgttggggc ccctgactag
    12661 aattgaaaaa agcttgttac aagcgcattt tcgttcagtc aactactgcc aatataactt
    12721 cgtagagcat agaacattga tttatgtccc cgcctctttt tatcgtatat agcatgtccc
    12781 tttttaaaat tacaagcata aatattcaga ctcaaatata gccacacccc taaactacgg
    12841 agggatgtgg ctgtcttttt tgattgtaat tatgtatcag atttaaatca ttaatgtaaa
    12901 gaggctaatt cattaataaa tttagtaaaa tctgatttag caacatttat ccttaatgaa
    12961 ataccagatt ctgttgccat gtttgaatgc cttaaaccag aatcaaaatc aatataaata
    13021 caaagcagtt cttgattaaa atcaataatt gtaaaatgta aatcaggttc tataaaatat
    13081 atttcagtaa caggattgtc gtaatccata tgtttcaaat tgtccatgtc actatcaaaa
    13141 agtgcaaatt caaatttaac ttcttgatct tcgaaagttg cactagtgtc gaaataataa
    13201 attttattat cttcttcgat aactacatct tttaaaataa atgttatcga tgttttgcta
    13261 tgtaaataag atgcatcttt taatatcaat ttagtaggct tcatcgtcaa attcctttca
    13321 tcattcattt tttcatgttg cataatcacc attactattg ataattctag gatagatact
    13381 cattgtataa taattattca ctagaacaaa aaaaacacac atcaatttgg ttgtttccat
    13441 ccaaaataat gtgtgtgttt atacatatat gattaattac taaacttcat cgtttatcaa
    13501 tattaaatcc attgatgcga tgttcagagt taacgagatt tcttctgtct atttgctctg
    13561 tctattaata cttgtttcgc ttgtatttcc gcttcattat ctatttcttt aggctcaaac
    13621 ggaataccct tacgttcaca agctttttct aacagataat cagtgatttc gtaattttta
    13681 ggtaaaattg gtccgtgtaa ataagtacct aataaatttt tataatgaat gccttctttt
    13741 ttatcttcat cattattacc ataaccaaaa gtgacatgac caagtgtacc gaaatcatga
    13801 tatgttctac caccgtgatt ttcaaaacct acaatagttc caaaagtatc actttcgata
    13861 acaatatctc ctgttaatcg gtttgtcttt gattcagtat aaaaatctaa aatacctaac
    13921 ccttctaatt ctgtaccatc aggcgtgata tatttttttc ctaaaaattg atagcctcca
    13981 caaattgtta atcccggcat accatcttca atcgcttctt taagtggtgt cttaatttta
    14041 cttaattctt ttgttgctaa tgcttgttct ctatcacttc caccaccgat aaagaaaata
    14101 tcacattcat caaaggtaat accttctgtt tcattgattt ctacgacatt aactttaata
    14161 tttcgttttt tagcacgttg tcttaaagca ataatatttc ctatatcact gtataaattc
    14221 aatttatctg acataaaatg ataaatagtc aattcatgca tattatgatt gacctccttc
    14281 aaacgaacgg tttaattgtt caagcatagg cgctaatgat gtatagtttg gtattgcaac
    14341 tgtgaaacct ttataatcca tagtctttgc cgttgcttta taaatatcac gctcaactat
    14401 aattggtact tcaacctctg ctaacttcaa tcgcaattga agttcttctg ctcgtgtacc
    14461 tgtcacgatg atagcttcaa tttgttgctt agataatttt tcaaaatctg catcataaat
    14521 ccatgaagta tctcgaccat ctgcagcgtt atcatttagc gaaataacat acactttttc
    14581 gccttctaat tgttcaccaa ctgataaact tgcattcatt cctgcaggat ttttagctaa
    14641 attgatcatc gcttcttttc gttctttttt aaagtactgc atacgaccat tgtctgatgt
    14701 atacgtttca aagccatttt taattgcttg ttcatttaac cctagctctc ttaaaacagt
    14761 atatgctgct aacgcgttat aagcgttaaa gtcacctgca attttcatat catatttttc
    14821 atcattgata tttaaatgta aaaacggtgc cacatcaaaa cttgatattt catattttgc
    14881 ttgctctcgt ttgaaaccac actgacagtg ataatgacca atttgattat aatgaatata
    14941 atcgtattgc aataagcgac cacagtttgg acaatatcta ctttcattca tcgtactttg
    15001 ttcaaattca tgggcatgtg ctttcatacc atagtacaca atcgtatcac ttgcgatttt
    15061 caaacgactc acaaatggat catcagcatt tagcaataat ttgatgcctt tattactaat
    15121 tgtctctgca atgttattaa ccataatatc aatttcaccg aagcgatcca tttgatctct
    15181 aaagaaatta gtaaatacca tcattgaagg tgtaacttct tttaacacac gtggaatcga
    15241 accttcatca atttcgatta ccgcaatttt agtcttaggt gttgattgca tgatgaatgc
    15301 agaagttata cctgcagcca tattagcacc ttcattattg tgtataattt gaatattatt
    15361 tgcttttaaa gtatgtccaa ttaagtttga agtcgttgtt ttaccatttg ttccactgat
    15421 aaatacaata tcatcaactt gctctgctaa ttttcttaat atatctgtat ccacttttct
    15481 agcgatttgt ccaggtaaat ctgttcctct tttacctact gctctacttg ctttacgcgc
    15541 caatttcgct agatggattg ccgtccactg tctcatgtgt ttcctcctca aatttccact
    15601 cgcatcatta taacatgaca aggcaacttc aaaaaagttt ctcaatcaca aattgataca
    15661 gtgtttctaa attataatca ttctcattta gataattaaa aatgatattt attctcaatt
    15721 aaatatcgct aaatgataca
//
LOCUS       DBNENW010000049        15643 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNENW010000049 DBNENW010000000
VERSION     DBNENW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.049"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            49..3501
                     /locus_tag="AC1LW8_001277"
     CDS             49..3501
                     /locus_tag="AC1LW8_001277"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HIP4773121.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEYGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(3641..4552)
                     /locus_tag="AC1LW8_001278"
     CDS             complement(3641..4552)
                     /locus_tag="AC1LW8_001278"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIP4773122.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            4992..5903
                     /gene="cyoE"
                     /locus_tag="AC1LW8_001279"
     CDS             4992..5903
                     /gene="cyoE"
                     /locus_tag="AC1LW8_001279"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIP4773123.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            5928..6389
                     /locus_tag="AC1LW8_001280"
     CDS             5928..6389
                     /locus_tag="AC1LW8_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIP4773124.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            6716..7753
                     /locus_tag="AC1LW8_001281"
     CDS             6716..7753
                     /locus_tag="AC1LW8_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HIP4773125.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            7769..8203
                     /locus_tag="AC1LW8_001282"
     CDS             7769..8203
                     /locus_tag="AC1LW8_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIP4773126.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(8266..9192)
                     /locus_tag="AC1LW8_001283"
     CDS             complement(8266..9192)
                     /locus_tag="AC1LW8_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIP4773127.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVSTKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            9431..9685
                     /locus_tag="AC1LW8_001284"
     CDS             9431..9685
                     /locus_tag="AC1LW8_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HIP4773128.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(9688..10077)
                     /locus_tag="AC1LW8_001285"
     CDS             complement(9688..10077)
                     /locus_tag="AC1LW8_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7147 family protein"
                     /protein_id="HIP4773129.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            10147..10689
                     /gene="rsmD"
                     /locus_tag="AC1LW8_001286"
     CDS             10147..10689
                     /gene="rsmD"
                     /locus_tag="AC1LW8_001286"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIP4773130.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            10691..11173
                     /gene="coaD"
                     /locus_tag="AC1LW8_001287"
     CDS             10691..11173
                     /gene="coaD"
                     /locus_tag="AC1LW8_001287"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIP4773131.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(11235..12374)
                     /locus_tag="AC1LW8_001288"
     CDS             complement(11235..12374)
                     /locus_tag="AC1LW8_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIP4773132.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            12501..13058
                     /locus_tag="AC1LW8_001289"
     CDS             12501..13058
                     /locus_tag="AC1LW8_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIP4773133.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            13138..13311
                     /gene="rpmF"
                     /locus_tag="AC1LW8_001290"
     CDS             13138..13311
                     /gene="rpmF"
                     /locus_tag="AC1LW8_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIP4773134.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(13428..14813)
                     /locus_tag="AC1LW8_001291"
     CDS             complement(13428..14813)
                     /locus_tag="AC1LW8_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001657254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP4773135.1"
                     /translation="MKVAIYTRVSTLEQKEKGHSIEEQERKLRAYSDINDWKIHKVYT
                     DAGYSGAKKDRPALQEMLNEIDNFDLVLVYKLDRLTRSVKDLLEILELFENKNVLFRS
                     ATEVYDTTSAMGRLFVTLVGAMAEWERTTIQERTAMGRRASARKGLAKTVPPFYYDRV
                     NDKFVPNEYKKVLRFAVEEAKKGTSLREITIKLNNSKYKAPLGKNWHRSVIGNALTSP
                     VARGHLVFGDIFVENTHEAIISEEEYEEIKLRISEKTNSTIVKHNAIFRSKLLCPNCN
                     QKLTLNTVKHTPKNKEVWYSKLYFCSNCKNTKNKNACNIDEGEVLKQFYNYLKQFDLT
                     SYKIENQPKEIEDVGIDIEKLRKERARCQTLFIEGMMDKDEAFPIISRIDKEIHEYEK
                     RKDNDKGKTFNYEKIKNFKYSLLNGWELMEDELKTEFIKMAIKNIHFEYVKGIKGKRQ
                     NSLKITGIEFY"
     gene            14922..15101
                     /locus_tag="AC1LW8_001292"
     CDS             14922..15101
                     /locus_tag="AC1LW8_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HIP4773136.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IKSEYKDTYLFRLIN"
     gene            complement(15081..>15643)
                     /locus_tag="AC1LW8_001293"
     CDS             complement(15081..>15643)
                     /locus_tag="AC1LW8_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773137.1"
                     /translation="GTVILQNNLDLFFNYGKRLANDHYNTLISKSKERLINKAHDEIQ
                     PFNHLDLNSMKEIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFLIKLKKVNK
                     IKHKDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAVAKGDCDFL
                     LNTLKDIVDENEKILREYTKSINKTE"
BASE COUNT         5273 a         2309 c         2770 g         5291 t
ORIGIN      
        1 ttatctaatt agttagaaaa ttctaatagt ttgaggagtg tatcgctatt gaaacaaata
       61 aaaaagttac ttgttgctaa ccgtggagaa attgcaattc gtatattcag agcggcggca
      121 gaattagaca tcagcacagt tgcaatttat tcgaatgaag acaaaagttc attacataga
      181 tataaagcag atgaatccta tttagttgga agtgatttag gtcctgctga aagttattta
      241 aatattgagc gtatcattga tgtagcaaaa caagcgaatg tggatgcgat tcatcctggc
      301 tatggatttt taagtgaaaa tgaacaattt gcgcgtcgtt gtgcagaaga aggaattaaa
      361 tttattggtc ctcatttaga acatttagat atgtttggag ataaagttaa agctcgtaca
      421 acggctatca aggcagattt accagttatt cctggtacag acggtccaat taaatcatat
      481 gaattagcaa aagaatttgc agaagaagct ggtttcccgc taatgattaa ggccacaagt
      541 ggtggcggcg gtaaaggtat gagaatcgtt cgtgaagaaa gtgaattaga agatgctttc
      601 catagagcaa aatcagaagc tgaaaaatca tttggtaata gtgaagttta catagagaga
      661 tacattgata atccaaagca tattgaagta caagttatag gtgacgaata tggaaatatc
      721 gtacacttat ttgaacgtga ttgttcagta caacgtcgtc atcaaaaagt tgtagaagtt
      781 gcaccatcag ttggattatc accaacatta cgtcaacgta tttgtgatgc tgcaattcaa
      841 ttgatggaaa atattaaata tgtcaatgca ggtactgttg aatttctagt atctggtgac
      901 gaattcttct ttatagaagt taaccctcgt gtacaagttg agcatacaat tacagagatg
      961 gtaacaggaa ttgatattgt taagacacaa attttagttg cagcaggtgc cgatttattt
     1021 ggtgaagaaa ttaatatgcc gcaacaaaaa gatattacaa cattgggcta tgccatccaa
     1081 tgtcgtatta caacagaaga tccgttaaat gatttcatgc cggatactgg aacaatcatt
     1141 gcttatcgtt caagcggtgg ctttggtgta cgtctcgatg ctggagatgg tttccaaggt
     1201 gctgagatat caccttatta tgattcatta ctcgtaaaat tatctacaca cgcgatatca
     1261 tttaaacaag cagaagaaaa aatggtacgt tcattacgag aaatgcgtat tcgtggtgtt
     1321 aaaactaata ttccattctt aattaatgta atgaagaata aaaagttcac aagtggtgat
     1381 tacacaacta aatttattga agaaacacca gaacttttcg acattcagcc gtctctagat
     1441 agaggtacta aaacattaga atatataggt aatgtaacaa ttaatggttt cccaaatgtt
     1501 gagaaacgtc cgaaaccaga ctatgaatta gcatcaattc caactgtatc ttcaagtaaa
     1561 atcgcttcat ttagtggtac gaaacaattg cttgatgaag taggtccaaa aggtgtagct
     1621 gaatgggtta aaaagcagga tgatgtttta ctaacagata caacctttag agatgcacac
     1681 caatcattat tagctacacg agttagaact aaggatatga ttaatatcgc atccaaaaca
     1741 gcggacgtat ttaaagatgg tttctcactt gaaatgtggg gcggtgctac atttgatgtg
     1801 gcatataatt tcttgaagga aaacccatgg gaacgacttg aacgtctacg taaagctatt
     1861 ccaaatgtat tattccaaat gttgttacgt gcttcaaacg cagttggtta taaaaactat
     1921 cctgataatg ttattcataa attcgtacaa gaaagtgcta aagcaggcat agatgtcttt
     1981 agaattttcg attctttaaa ctgggtagat caaatgaaag ttgccaatga agcagtacaa
     2041 gaagcgggca aaatctctga aggtactatt tgttatacag gtgacatttt aaatcctgag
     2101 cgatcaaaca tttatacttt agagtattat gtcaaactag ctaaagagtt agaacgtgaa
     2161 ggtttccata ttttagcgat taaagatatg gcaggcttat taaaacctaa agccgcttac
     2221 gaattgattg gtgagttaaa atcagctgta gatttaccaa ttcatcttca cactcatgat
     2281 acaagtggta atggtttatt aacatacaaa caagcaatag atgctggtgt cgatatcatt
     2341 gatactgctg ttgcttcaat gagtggttta acaagtcagc caagcgccaa ttcgttatat
     2401 tatgcattaa atggcttccc acgccacctt agaactgata ttgaaggtat ggagtcactt
     2461 agtcattatt ggtcaactgt acgtacttat tattcagact ttgaaagtga tatcaaatca
     2521 ccgaatactg aaatttatca acatgaaatg cctggtggac agtattcgaa tttaagtcaa
     2581 caagctaaaa gtttaggttt aggcgaaaga tttgatgaag tcaaagatat gtatcgcaga
     2641 gtgaatttct tatttggtga tatcgtaaaa gtaactccat cgtctaaagt agttggtgat
     2701 atggcacttt atatggtaca aaatgatctt gatgaacaat ccgtgattac agatggctat
     2761 aaattagatt tcccagaatc agtagtgtcg ttcttcaaag gcgaaatagg acaacctgta
     2821 aatggtttta ataaagattt acaagcggtt attttaaaag gccaagaagc actaacagct
     2881 cgtccaggtg aatatctaga gccagttgat tttgaaaaag tccgtgagtt gcttgaagaa
     2941 gagcaacaag gtcctgttac ggagcaagat attattagtt atgtattata tccaaaagta
     3001 tatgaacaat atattcaaac tagaaatcaa tacggaaact tatcgttact tgatacgcct
     3061 acattcttct ttggaatgcg taatggtgaa acagtagaaa tcgaaatcga taagggtaaa
     3121 cgattaatta ttaaactaga aacgattagt gaaccagatg aaaatggtaa tagaacgatt
     3181 tactatgcga tgaatggtca agcgagacgt atttacatta aagatgagaa tgtgcataca
     3241 aatgcgaacg ttaagccaaa agcagataag agtaatccaa gtcatatcgg tgcgcaaatg
     3301 ccaggttcag taactgaagt caaggttagt gtaggtgaat ctgtgaaagc taaccagccg
     3361 ttgctaatta ctgaagctat gaaaatggaa acaacaattc aagcaccatt tgacggtgtg
     3421 attaaacaag taactgtaaa taatggtgac acaatagcga caggcgattt attaatcgaa
     3481 attgaaaaag caactgacta aaatgattaa aataaaacga gattacacaa caattgacgt
     3541 gtaatctcgt ttttacatgg cttaggttta attttaaata ttgatataaa aaaccgtaat
     3601 tttaatgggc gtgccattaa aattacggtt tgcttttttg ttattgcttg tcacttctta
     3661 ctgatcgtaa cattagcatg ataaagtatg ttgtcatacc aaataaataa gtgataaata
     3721 atgcatggaa aagtgcgatt atcaggttaa catttgtcat aatagataat gcacctgtga
     3781 taacttgtaa tataacaagt ataaatgcag ctgtataacc ataatgaaca gtacggttat
     3841 ttggataatt tttaacagcg tgaatataag taatcataat aatcgtaaac actataaaag
     3901 ccatgatacg atgcgtgagt tgaacccaat cttgttctga atgtggtaca agatcgtgga
     3961 atggcaatgg ccaaccacca tatgctaaac ttgcatccgc atgtctcact agtgcaccag
     4021 tataaacacc acaataaatg atgattgcca ttaaccatgt taaacgtctt aatggctttt
     4081 tgatatataa ttcgtcagct tcatattttt gatctataga gaaaataatc aatgttatta
     4141 aaaatacaga tgagaaactg attaatgata taccaaagtg caatgctaaa acgtaatcgt
     4201 tttgttgcca aataacagca gcagctccga ttaatgcttg caataataag aatccaacac
     4261 taatgattga taaaggttta atttctttaa tatagcctat atgtttccat gcagtgataa
     4321 ctaaccataa gaccattaat aaagacaaag ctgaaacggc tctatgactt aactcaataa
     4381 tcgtatcaat aggaaagaat tctggaatca acgcaccatg acatagtggc caagaagaac
     4441 cacaaccatc agctgatccg gttttggtaa ctaaggctcc accaagttgt acaaatgtca
     4501 tcattaacgt tgctacgaca cctaaccatt ttaaattctt tttgccaaac aatttataca
     4561 ccccatcata aaaaaacgca tcactgagaa ttagaattaa tagccggatt tgagatgata
     4621 ctataaattg acctgattgt aggatatgaa aagatgggat ccaacgcagg aaatatgatt
     4681 taattttaca agtattgtat gtggacgaca taaaaagaac ttaatcgtaa tgattttttt
     4741 attgggataa ttatgtaatc cacgtttact caaatttgaa gcttaaatat taatctcttt
     4801 tagatgcatg attctactta ttaaaatttt ctaacatata cattatagca ataaattaaa
     4861 tgttatttgt gtcacaaaat tgacattttc tcttgtcgtt aatttgtcac aatttaattt
     4921 tattaaacct agtcttttta tgcaatctaa gatatcataa tcttatatga acaaatttaa
     4981 ggagggggat tatgagcaaa gagcatactt tgtcacaaaa tattagcaga gttaacttca
     5041 aagaattgca acagataatt aaaatgggac ttgttcaagg taacttaatt ccggcttttg
     5101 cgggcgcatg gctagcagtt gtaatgacaa atcattcctt cttatcatca atacctcaaa
     5161 ttttattaat gctattagga tctactttaa ttatgggtgg cgcatgtgcg ttaaataatt
     5221 attacgacca agatattgat cgtattatgc ctagtaaaca aaatagacca actgtaaata
     5281 atagaattac agatcaaaat ttattattat taagttttgg tatgatgtta gttggagaaa
     5341 tttgtttgtt tttattgaat ataccatcag gcgtacttgg tcttatgggg attgtaggtt
     5401 atgtgtctta ttactcaata tggtctaaaa gacatacaac atggaacaca gtgattggga
     5461 gttttcctgg agcagtacca ccactaattg gatgggttgc aattgaagga caaattagtt
     5521 taacagcgat tgcgctgttt ttagttgtat tttgttggca accaattcat ttttatgcct
     5581 tagctattaa acgtaaagat gaatatgcac ttgcaaatat tccaatgtta ccatcagtta
     5641 aaggctttaa acgtacacgt gtcagtatgt ttatctggtt gattatttta ttgccagtac
     5701 ctttattact aataaattta ggtgtcgtat tcgtagtgtt agctacatta ttaaatttag
     5761 gatggattgc attaggttta acaacattta agaaaaattc agatcaaaca aaatgggcaa
     5821 cacaaatgtt tatatattca ctaaattatt tagtgatctt tttcgtgtta gctgtgattg
     5881 tttcattact tactttgatc tagattaatt aagttaggat gaaaaatatg ggcgttccaa
     5941 ttttaccaac gattagtacg acatgtattg tcattagtgc aattttaatt gccattggtt
     6001 ggagacttat ttggaaaagg gaaataaata aacacaaaaa tgttatgtta gctgcagctg
     6061 tttttgcttt aacttttttc ttaatctatg caagtagaac gattttcatc ggtaatacag
     6121 catttggcgg accagcatca attaagaaat attatacgat tttcttattt ttccacatta
     6181 atttagcaac aattggtggt attctaggcc tagttcaaat tattactgca tttaaagata
     6241 aatataatgt acaccgcaaa ttcgggccat tcgcttcagt tatatggttc tgtactgcaa
     6301 ttacaggtgt agcagtttac ttattattat atgtattata tccaggtgga gaaacgacat
     6361 cactgattaa agcaacattt ggtcattaat atgcttatac aaatgatact aatctgataa
     6421 ggcaatttgt tttatcaggt tttttgatga agaatagtcg gacagttgat ggtattcatt
     6481 tgatacctta actgtccatt tttttataca tattttaatc ctaataatga aattcaacct
     6541 gaaaacataa agataggtta tgatatagag tgtagggaaa atctttatga cgttaaaatt
     6601 atgcctgaag atgattttgt ttcatcgaaa cgacacagtt atgtcagtgt tgaaaatttg
     6661 ctaattaatg tgttgttttt tacaatagta ttaatacttt ttaggtggtt gattgatgaa
     6721 aaagttaatt ataagagtcg tcggtgtatt atttttagtt ggatttttaa tatatctttt
     6781 ttattcaccg agattaaaat ttgatgtatt agagaacccg aataaaggta ataaagtaaa
     6841 tagatctgaa caggtgaata aatcaaataa ccatgctgaa aatccaaagc ctaaagaagg
     6901 tgttggtaca tgggtaggta aagatattaa agtgcttact tctaaatttg gacaagcaga
     6961 tcgtgtctac ccttttagag atggttacaa aaattatgtg tttaaagaca aaaacagtta
     7021 ttacattgtt tcaactaaac gtgaagaaat cgtttcagtg tatgctacag gtgagaaagt
     7081 caatgttagt ccgttaaaaa taggacaaca ttctgcagaa atttttaatc atacaagtat
     7141 taatccagaa ccgtccttta aagttgatgg taaaaaatat gaatttgaac tttcagatga
     7201 agatttaaaa acacaaacac tgattaaata tggcgacata tatgcacaag tgtattctga
     7261 tcaacaatcg aaaaaggtgc taagtgtacg atttttaaca aaagaaatgt tagcagatat
     7321 tgaaccttat caattaaatt ctaattctac gtcagaagag cataataagc gtccagttga
     7381 gcaaaatcca aatcaattaa tttctcttta tgaagtaacg aatgaaatga gaaaattaaa
     7441 aggattaaaa ccattgaaaa tcaatagcga tttagcacat attgcatcta ataacttata
     7501 tgaagcgacc tctaatggtt ctgatagtgt tgaatttaca gaggacgcat taagagggca
     7561 attagataaa aatcacgtta cttataaaac aactgctcaa aatgttggtt atgcgtttaa
     7621 tgatgtacca acattaatcc atagttggat gaattcagat atacatcgat ctcgtctatt
     7681 aaattcaaaa tacgatgaga tgggtggaga tgtaatgaga gattattatt cactaatttt
     7741 cttagaaaaa taacaatgag gggtatttat gattaatgaa gcttcactag cgatattaga
     7801 tgatattgat gaactagctg atatgatagt tgcatcagat atttatgcat catttgagca
     7861 agctaaacag gcgctcgaaa acaatgacga agcacattta ctttatcaat catttttaaa
     7921 atcaaaagag aaatatgatg aagtaatgcg tttcgggaaa tatcatcctg attataagaa
     7981 agtcatgtta gagacaagac aacgcaaaag agcatatgag atgcttgacg ttgtgatgca
     8041 ttacaaagct agggaaatgg cacttcaaca tttaattgat gaagttgtta cgaaaattgc
     8101 gtacgctgtt tcagagcatg tcaaaataga aacaggtaat ccattctttc aaacatcaca
     8161 tagtggttgt gcgacgggcg gatcctgtaa ttgttcatta taaaaaacat cgagtcagaa
     8221 aaaggtggtt attgaaacca ctaactagca tctgactcga tgtttttatt tattcgggat
     8281 tgtttgtttg aattgttgtg ctaaatctgg tcgatctgtc acaatcgtgt gtgcaccttt
     8341 ttggtataaa tcattcatca gatctatact atttacgcca taatagcctg gaatgatatt
     8401 catatcattt aaccatttga taaaacgaga tgaagtcaaa tcaatgcctt taaaatgagt
     8461 aggcatttgg aacgtttgtg ctaatggttg gtagtatcta ccacctaata aatgatattt
     8521 taaaaatgct tctgtaactt cctgttggct agcaccaatt gcgacggatc cttgtgcaat
     8581 tttattaaaa cgaacgattt gttctttata aaaacttgtc acaagaacgc ggtcaaatgc
     8641 ttgattttct gcaattgtat caaacataat ttgtggtgcg attgagcctt cataggattc
     8701 aggagcatct tttaagtcta cgtttatata catatcagga tattgcttca gcaactcatc
     8761 gaaggttagt atagctgtgt gtgcatgacc acgatatggt gttagtccat tgatatcttt
     8821 gaagtgataa gctgcgtcta atttctttaa ttctgctaat gtatgggcac taacttttcc
     8881 agagccgttc gtcgttctat caacagttgc gtcatgaaaa acgataagct gttgatcttt
     8941 tgtgagtctc acatccgttt caaagccatc aacgcctaat tgtttagcat agtcaaatgc
     9001 aagttgcgtt tgctctggtc ttaaagccat accaccgcga tgcgcaaata tatatggtgc
     9061 attgcctttg aaaaaagcag ggatggtttg ctttttagta ctcactttat ttttattgat
     9121 cattaataga ctacttacaa atccagcacc gactagtacc gcatttaaaa tgtttctgtt
     9181 tacttttttc atagaaaatt cctcctcgtt tacttttata gcttaacaaa atcatagttc
     9241 tgactacagt gaaatgtgtg gttaatcaaa tagtgatgct cttgagaatt gaattaatat
     9301 aatgatgttt aatataatct aaatacattt atcgagcaat tacacaagac atatttaagg
     9361 tagcttaata ttttaaaatt aataaagaca tgatatgatt aatgctgaaa ataaatggag
     9421 tgaggtttaa atgaatttaa tcccaagaac tagtattgta gtttatttaa aacatatgaa
     9481 acatgaacga caaatccgaa aatatggaca tatcgttcat tcaaatagag atcgtaaatt
     9541 tgtaattatg tatgtgaatg agcaagatgt tgatcaaatt gtacataaac taatgcaact
     9601 taaatacgtt agacacattg atggctcacc atataaatac ttaaagaaaa cttacgaaaa
     9661 agagaaacac gaaatatata attaatatta ttgaagttct ggtatccatt tttggagcct
     9721 taacacacta attaaataat tataatataa cgataaatca tgaaatgctt gagggggctg
     9781 tacatcgata cctaaaattg gtatgtcgaa tttttcagca caatcattaa tcatttgaaa
     9841 ctttttattt gaatctggat atggatattt aatagcgtta atcatgatat aaaatgcttg
     9901 gaaatgattg ccagttgtag ggttcataat gattgctaca gatgactttt tattttcagc
     9961 ttgtggtgaa tgaaaataga taattttatc aatacgttga tggttatatt caatcatcgt
    10021 catgaattcg aacaaatctg ttagaccttc accaagtgta ataaatgttt gtttcattgg
    10081 tttatcattc tcctttttct caataaatta tattaaataa ctaaatgaat tggaagtgtg
    10141 aacaacatgc gcgtcattgc aggtaaacat aaaagtaaag ctttagaaag tatggaaggc
    10201 cgtaatacga gaccaactat ggataaagtt aaagaaggta tctttaatag tttatatgat
    10261 gtgtcaggta taggtttaga tttatttgca ggaagtgggg cgcttggaat agaagcactc
    10321 tctcgaggta tggataaggt aatctttgtt gatcaaaatt ttaaagctgt aaaagttatt
    10381 aaatcaaatc ttgcgaattt ggatttagag gcacaatctg aagtttataa aaataatgca
    10441 gatagagctt taaaagcatt gtcaaaacgg gatattcaat ttgatgtcat tttcttagat
    10501 ccaccttata ataaaggtct cattgataaa gctttaaaac taatttcaga gtttaattta
    10561 ttgaaagaaa atggtatcat cgtttgtgaa tttagcaatc atgaagaaat agactatcaa
    10621 ccgtttaata tgattaaacg ttaccattat gggttgacag acacattgtt attagaaaag
    10681 ggagaatagc atggaacata caatagcggt cattccgggt agttttgacc ccattactta
    10741 tggtcattta gacattattg agagaagtac agatagattt gatgaaattc atgtctgtgt
    10801 tcttaaaaat agtaaaaaag aaggtacgtt tagtttagaa gagcgtatgg atttaattga
    10861 acaatctgtt aaacatttac ctaatgtcaa ggttcatcaa tttagtggtt tactagtcga
    10921 ttattgtgaa caagtaggag ctaaaacaat catacgtggt ttaagagcag tcagtgattt
    10981 tgaatatgaa ttacgcttaa cttcaatgaa taaaaagttg aacaatgaaa ttgaaacgtt
    11041 atatatgatg tctagtacta attattcatt tataagttca agtattgtta aagaagttgc
    11101 agcttatcga gcagatattt ctgaattcgt tccaccttat gttgaaaagg cattgaagaa
    11161 gaaatttaag taataaaaat aacagtattt taggtttatc atggtttaca atcctaaaat
    11221 actgttttca tttgttaacg atattgctgt atgacaggcg tgttgaaatc tgtttgttgt
    11281 tgcccgctta ttgcattgta tatgtgtgtt gctttgattt catttgtgaa gtaatgtgca
    11341 ttgcttttgt taatattggt tatatattgt ctttctggga acgctgtttt taaatgcttt
    11401 aaatattgtc tgccacggtc gttcatcgct aatactttaa ctgcgtgaat gttactcgta
    11461 acatctgtag gtttaatgtt taataataca ttcattaaca gtctttggat atgcgtatat
    11521 gtataacgct ttgtttttag taattttaca aaatgatgaa aatcagttgc ttcataaatg
    11581 ttagatttca aacgattttc aaaaccttca gtaacagtat aaatattttt taatgaatct
    11641 gtagtcatag ctatgatttg atatttcaaa tatggaaata tttgatttaa tgttatatga
    11701 ggtgttacgt acaagtgttg aatatcttta ggtaccacat gatgccaatg atcatcttga
    11761 ctaatgattg atgttctaat agatgtacca cttgcaaact gatggtgttg aattaatgaa
    11821 tcatgatgtt gagcattttc tcgtttgata gaaattgcat tgatgttttt agcattttta
    11881 gcaattgctt tcaggtaact aataccaagt atgttgttag gacttgctag tgcttcatga
    11941 tgctctaata attcgctaat gatacgaggg tagcttttac cttcttttac tttttgtgaa
    12001 aaggattcag attgttcaat ttcattaatg ctgtgtgcta attgctttaa tgttttgata
    12061 tcattatttt cactaccaaa tgcaattgta tcgacactca tataatctgc gactttaact
    12121 gctagttcgg caaaatgatc gccagatgat aaactggcag ttgctggtag ttcgataact
    12181 aaatcagctg ttgataacgc catttttgca cgagtaaact tattatagat tgctggttcg
    12241 ccacgcatga caaagttacc actcattatt gcaatagtaa cgtcagcatt tgtaagtttt
    12301 ttagattgat taatatgata ttgatgccca ttatgaaagg gattatattc tgtgattaag
    12361 ccaacgcttt tcatttgcaa ttcgtccttt cggtattcat tattaatatt gtaacagaac
    12421 atgatatgtt aagaaaaaat cttgacaact tgttcttaga aagttaaaat aaattttgtg
    12481 cttgttagag gtgaagccat atgaaatggt caattacgca attaaggaaa tatcaaggta
    12541 agccatttga atttgatcaa acggtgagtt ttgacaattt aaaagaatca ttagatttaa
    12601 ttgatttatc tccaattaca atccaaggtc agttaaccat taagtcaaca gaagtcgttg
    12661 cggatattca cattactgga acgtatacaa tgccttgtgc acgtactctt gtaccagtaa
    12721 aagtcccact agatgtaact actacagaag tatttgattt agaagggtac aatcagtata
    12781 acgatgatca agatgatgta gatgaacact atcacattat taaagatggt atggttaatc
    12841 ttcaggatat tgtcgaggat atagttatta ttgagaaacc aatgagagct tattcagagc
    12901 aaagtgacca aatgttgaca gtaggtaatg gttgggaagt aatcgatgag gatcaattag
    12961 atgagcttgc taaacagcaa gaacaagatg attcagaatc acgacaagtt gatccaaggc
    13021 ttcaaaaatt acaacaatta tatgataaag agcaataagt agtttaatta atgatataat
    13081 gttgttaatt aaactaacat ttagtatttt tcgtttataa tctaaggagg atatatcatg
    13141 gcagtaccaa aaagaagaac ttctaaaact agaaaaaaca aacgtcgtac gcatttcaaa
    13201 atttcagtac caggtatgac tgaatgccca aactgtggcg aatacaaatt atcacaccgt
    13261 gtatgtaaaa actgtggttc ttacaatggc gaagaagtag cagctaaata attttagtta
    13321 ctcaatataa aaagtcccgc ttaaaatgat tgttttaagt gggacttttt atattgcgaa
    13381 aaataattgg cgaacgaggt aactgcatag ttattccgaa cttccaatta ataaaactct
    13441 atacccgtaa tcttcaatga gttctggcgc ttccctttaa ttccttttac atattcaaaa
    13501 tgaatgtttt tgattgccat ctttatgaat tcagttttta actcatcttc cattaattcc
    13561 cagccgttta gcaatgaata cttgaaattt ttaatcttct catagttaaa agtcttaccc
    13621 ttatcattat ccttgcgctt ttcatactca tgtatttctt tgtcaatacg acttattatt
    13681 ggaaaagctt catccttatc catcatacct tctataaaaa gtgtttgaca tctagcgcgt
    13741 tcttttcgca acttttcaat atcgatgccg acatcttcta tttctttagg ttggttttcg
    13801 attttatatg atgttaaatc aaattgtttt agataattgt aaaattgttt taaaacctcg
    13861 ccttcgtcga tgttacatgc atttttattt ttagtatttt tgcagttaga acaaaagtat
    13921 agtttagaat accaaacttc tttattttta ggcgtatgct tgactgtgtt taaagtcaat
    13981 ttctggttac agtttggaca taatagttta cttctgaaaa tagcgttatg ttttacgatt
    14041 gtagagttag ttttttcact tatccttaat tttatttctt cgtattcttc ttcacttata
    14101 atagcttcgt gggtgttttc gacgaatatg tcaccgaaaa caagatgacc tctagctacc
    14161 ggactcgtta gagcattgcc tataactgat ctgtgccagt ttttacctaa gggtgctttg
    14221 tatttagagt tgttcaattt tatagttatt tctcttaaac tagtaccttt tttcgcttct
    14281 tctactgcaa atcgtaatac ttttttatat tcattaggca caaatttatc atttactctg
    14341 tcgtaataga aaggagggac agttttagct aacccttttc tagctgatgc gcgtcgaccc
    14401 attgcagtac gctcttgaat tgtagtacgc tcccactctg ccatagcacc tactaatgtt
    14461 acgaacaaac gtcccatagc agaagttgtg tcatatactt ctgttgcgct cctaaacaac
    14521 acgtttttat tctcaaacaa ttctagtatc tctagtaagt ctttaacact tcgagttaat
    14581 cgatctagtt tatagactaa aaccaaatca aaattatcta tttcattcaa catttcttgt
    14641 aaagcgggtc tgtctttttt agctccggag tatccagcgt cagtatatac tttatgaatt
    14701 ttccagtcgt ttatgtcgct gtaagctctt aattttcttt cttgttcttc gatagagtgt
    14761 cctttttctt tttgttcaag tgtactcact ctagtataaa ttgctacttt catgtgctcc
    14821 ctcctcaaaa ttggcaaaaa ataataaggg taggcgggct acccgaaatt ttagtactag
    14881 ctactaaatg tgatataata aaataaaaag taggtgatga aatgtgtgta aaatttactg
    14941 acgcagaaat agcttatata aaagaatcag ttgaaaatta tagtagtgaa tttgatattt
    15001 atgacgatga acaagaactt aaattaaaaa tttatgaaca aattatgtta aaaatcaaat
    15061 ctgaatacaa ggatacctat ttattccgtc ttattaattg atttggtata ttctcttaat
    15121 attttttcgt tttcatcaac aatgtctttt agtgtgttta aaagaaagtc acaatcacct
    15181 ttggctactg caccagcttg tgaatggttg attatgtttc tcatactata cgcaatttct
    15241 acccgttttt tggttctata atttacttta ccttctttag ttaattctcc taataatttt
    15301 gtgtacatag ttgaatcggt gtctttatgt ttgattttat taactttttt taatttgatt
    15361 aaaaacgttt ctatagcaac agcaaaggtt gctgcagctg gcaaatacaa ttccctttta
    15421 taagcttgta atccttgttc tatttgataa gaaaaagtta tatcatcaac aatctctttc
    15481 atactattta aatctaagtg gttgaacggt tgtatttcat catgtgcttt gtttatcaat
    15541 ctttctttcg acttcgatat caatgtattg taatgatcgt tagctaatct tttgccataa
    15601 ttaaaaaata aatctaaatt gttttgtaat attacggtcc cga
//
LOCUS       DBNENW010000050        15523 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNENW010000050 DBNENW010000000
VERSION     DBNENW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.050"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(8..1300)
                     /gene="asnS"
                     /locus_tag="AC1LW8_001294"
     CDS             complement(8..1300)
                     /gene="asnS"
                     /locus_tag="AC1LW8_001294"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIP4773138.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKSEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(1622..4315)
                     /locus_tag="AC1LW8_001295"
     CDS             complement(1622..4315)
                     /locus_tag="AC1LW8_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HIP4773139.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRSENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(4339..5310)
                     /locus_tag="AC1LW8_001296"
     CDS             complement(4339..5310)
                     /locus_tag="AC1LW8_001296"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIP4773140.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSVQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(5297..6499)
                     /locus_tag="AC1LW8_001297"
     CDS             complement(5297..6499)
                     /locus_tag="AC1LW8_001297"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIP4773141.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVIKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(6504..7646)
                     /gene="bshA"
                     /locus_tag="AC1LW8_001298"
     CDS             complement(6504..7646)
                     /gene="bshA"
                     /locus_tag="AC1LW8_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIP4773142.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYGLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(7888..8205)
                     /locus_tag="AC1LW8_001299"
     CDS             complement(7888..8205)
                     /locus_tag="AC1LW8_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIP4773143.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(8541..9221)
                     /locus_tag="AC1LW8_001300"
     CDS             complement(8541..9221)
                     /locus_tag="AC1LW8_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIP4773144.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILLARSSD"
     gene            complement(9293..9880)
                     /locus_tag="AC1LW8_001301"
     CDS             complement(9293..9880)
                     /locus_tag="AC1LW8_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HIP4773145.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILFLVISISMILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLILMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(9870..10445)
                     /locus_tag="AC1LW8_001302"
     CDS             complement(9870..10445)
                     /locus_tag="AC1LW8_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HIP4773146.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(10459..11703)
                     /locus_tag="AC1LW8_001303"
     CDS             complement(10459..11703)
                     /locus_tag="AC1LW8_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIP4773147.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIAKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLRQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLTEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(11710..13008)
                     /gene="aroA"
                     /locus_tag="AC1LW8_001304"
     CDS             complement(11710..13008)
                     /gene="aroA"
                     /locus_tag="AC1LW8_001304"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIP4773148.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNISIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(13018..14082)
                     /gene="aroB"
                     /locus_tag="AC1LW8_001305"
     CDS             complement(13018..14082)
                     /gene="aroB"
                     /locus_tag="AC1LW8_001305"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIP4773149.1"
                     /translation="MKLQTTYPSNNYPIYVERGAIDHISTYIDQFDQSFILIDEYVNQ
                     YFANKFDDILSYENVHKVIIPAGEKTKTFHQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHEINHYIQYLIQLGYPLDMITDLDFETLYEYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(14108..15274)
                     /gene="aroC"
                     /locus_tag="AC1LW8_001306"
     CDS             complement(14108..15274)
                     /gene="aroC"
                     /locus_tag="AC1LW8_001306"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIP4773150.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSKTFKANLDRNDVRVIDDSIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         4926 a         2859 c         2095 g         5643 t
ORIGIN      
        1 ctaaaactta tggatataaa cggtttaata atcttgggaa tggcgctgtt tcacgaacgt
       61 gttcaacacc agaaatccat gctactgtac gttctaaacc taaaccaaat ccacagtgtg
      121 gcacactacc ataacgacgt aagtctaagt agtaactata tgcttcttcg tctaatccat
      181 gttctttaac gcgttgttct aacaattcta agtcatccac acgttcagat ccaccaataa
      241 tttcaccgta tccttcaggt gcaattaagt ctgcacataa tacagtttct tcattttcag
      301 gatttggttg catatagaaa ggcttaattt tagttggata attagtaata aacaccggta
      361 aatcataatg attagcaatg gctgtttcat gtggcgcacc aaaatcttcg ccccattcaa
      421 tatcatcaaa gccttctgat tttaagaatt caattgcatc atcatatgaa attctaggga
      481 atggtgtcgc aactttttca agttttgatg tatcacgctc taaaattttc aactctagtt
      541 tacaattttc taaaactgat tttacaacat gtgttacata ttgttcttga atttctaaac
      601 tttcagcatg atttgtgaaa gccatttccc cttcaatcat ccagaactcg atcaagtgtc
      661 tacgtgtttt tgatttttca gctctgaaag ttggaccaaa tgaaaatact tttccgtgtg
      721 ccattgctgc agcttctaag tataactgac cactttgaga taaaaacgca tcttgatcaa
      781 agtatttagt atggaataat tcacttgtac cttctggcgc acttgctgtc aaaattggtg
      841 gatcaacctt tgtaaatcca tctttgttga aaaattcata cgttgcacga ataacttcat
      901 ttctaatttt cattacagca tgttgttttt tagaacgtaa ccataaatga cggtgatcca
      961 ttaagaattc tgtaccatga tttttaggtg taatcggata gtcatgcgct tctgaaataa
     1021 cttcaattga tttcacttgc atttcgtatc ctaagtcaga acgattatct tctgtaattg
     1081 tgcctgtaac gtatagagat gattcttgag taatttcttt cgcaagtttg aatacctctt
     1141 catcaacttc tgatttaact actacgcctt gcataaagcc tgttccatca cgtaattgta
     1201 aaaaggcgat tttaccactt gaacgtttat ttgttagcca agcaccaatt gtaacgtctt
     1261 ggtttaaatg atctttcgct tgtttaatcg ttgttttcat aaccattctc ctatttattt
     1321 tttcgttata caatactcat tcattttaac aaaatccgct ttcaagttct agaactagac
     1381 taaaagataa cgtgtaaatg gtaatgattt aagcacagat ttgaacatta atttatatta
     1441 agacaataca atcatcagtt tcagacaaaa tatataataa agcctcaata ttattagtat
     1501 gaaggttgtt ttttgtgcta acattcaagt ttaattataa aactcaaatt ttgacgacca
     1561 aacaaaaatt cttgtaacag aacatagcac atgtcatgtc acaagaattt tactttgtat
     1621 ttcacttttt ctttttttga atttgtctta ataattttcc aaactgttga atgtcgcctt
     1681 ttttctgacg ataattttca agtgtttgtt caaaaaagtt tttataatta ctgtttataa
     1741 gtcgatcatc aaatgaaact attatgccgc gatcattttc acttctaatt aatcttccaa
     1801 gtccttgtct aaaacgtgta actgcatcag gtaatacata ttccttgaaa gttgaagtga
     1861 attcagaatc cataagccaa tattttgcat tatgcttgtt cataaacggt aacttcgcta
     1921 tcatcacaca tttaatacca tttgcttgaa aatcaaaacc ttcaaaaaat gttgacgtac
     1981 caagcagtat ggccttatca aaattattaa actgttgtac tattttataa ttttggttct
     2041 gctgttgtgt taatacaaca taatcttcaa attctggcaa ttcatttagc atatcttgta
     2101 ccatatgcat cattttataa ctcgtaaata agactaaaca ttttgatgac gttatagtcg
     2161 tatattcaat aatatagctt acaatcgatg ctacatactc atctatattt ttatattgat
     2221 aagatgctac atcactcggt ataaatacac ttgtattttt tgcactttgt aacgacgtgt
     2281 taacttcaaa tgtattaaag tgaacatctt tgttgaataa ctgtttaaaa gcttcaaacg
     2341 aatgattaaa ttttaaagta ccagatataa aaatgagtga tttaaatttt tccaatactt
     2401 gtttcgttaa tacatctttt acagcgtaat ctttcacata taaacgtatt gttgattttt
     2461 gagataaatt tttaatcgaa atgaaactag tatgtccagc ttttaaactt tgttcgatat
     2521 ttttaaattt atcttttaaa tacaacaact gtttccgtaa tgatttaact gttttatgac
     2581 taatgccatt gaatatttct agcgttttat ttaacttatc gataatcgca tgtaaatcct
     2641 tcaaaatgtc ttttgtttca aaagtaaata cattatggaa gcgatgaata tcatcatcat
     2701 aaacatcaga atcattgata atcgtaaata tcgttgagaa caattgctca tttaactcat
     2761 gaatctcatt catactagcc ttcaagccaa aaatatcaat tggtgcaata tctaattttt
     2821 ccaaaattcg ctgcttttcc agttgatcaa ttgcctttaa caatttttca ttttcgtttt
     2881 taccaatcaa accaagctga tatttaatat cagcataact caactcattt gtcacttgat
     2941 ttaaggcata gtctggtaag cgatgtgctt catccactat acaatcatca aacaattgat
     3001 atattgaatt ttcaacatca gaatgaatta aatgtgcatg atttgtaata ccaatttgaa
     3061 tgttctgtgc atttcgctta ataaaattat aataatgaac atcgtgacgt gccggtacat
     3121 atgtttcaat tttctggtca aaatacatct tttgaccacc ttttaaattt aattcctgta
     3181 tatctccgga cggcgtctct gtaatccaaa tcagcaattg cattttcaag atattcactt
     3241 cgtaattact tgtgtcatct tttaaaattt gactaataag ccccaatgaa atgtaatcac
     3301 ttttactttt aatcaatagt gcattaattt taaaattcaa cgcttcattc attgctggaa
     3361 tatctttttc taacaattga ctttgcagta atttagtatt ggtagaaatc atgacatgct
     3421 tcccagtttc aatattatac atcaaggccg caagtaaata tgctaatgat ttaccactgc
     3481 ctagtgatgc ttcaatcatt gctttttcac tatgcatgag ctgatctaat atagtttccg
     3541 ctaaatataa ttgttgcggt cgatatgtta agccaagttg atctacagct ttgctatata
     3601 aagacttcaa gctgccatta taatttgttg tcggcttttt aaaatcaact tgcttacgat
     3661 agataatctg ttcgaacttt tcgtacgatt tatccaatgg ctttgcatca tattgcctaa
     3721 ccatctcaaa gaaaatatca tacaaatcgt atttcaactg tttacttaaa taatataatt
     3781 gcttcaaagt atctaacggt aacttttcaa atttttcaaa agctaatatc atcaatttag
     3841 cagtagtagc ggcatcttcg tcagctcgat gggcatttgc taaggtaata ccatgtgcct
     3901 ctgctaattc acttaattga tagcttttat ctgtaggaaa agctatttta aagatttcta
     3961 gtgtatctat aacttttttg ggacgatatt gaatattaca atctttaaat gcctttttaa
     4021 taaaattcaa atcaaaatct acattatgag ctacaaaaat gcaatctttt atcttatcgt
     4081 agatttcttg tgcaacttga ttaaaatatg gcgcttgttg tagcatattt tcttcaatgg
     4141 atgttaacgc ttgaatgaac ggcggaatct ctaaatttgt tctaatcata gaatgatatg
     4201 tatcaataat ttggttattg cgcacaaacg ttataccaat ttgaatgata tcgtcaaaat
     4261 ctaattggtt gcctgttgtt tccaaatcca caacggcata ggttgccata cccatagcta
     4321 tctctccttg ctttagtgtt aaaaatctat atctgcacta attaaacggt gtgattcacc
     4381 cgcttcatct ctaacaatta gatagccatc gtaatctaaa tcaattgctt gtcctttaaa
     4441 ctgtttatca ttttctgtaa atagcaacgt tctattccaa atattagaag ctgcaatata
     4501 ttcttcacga atttcagaaa aaggtaacgt taaaaattga ttatatcttt tttcaatttc
     4561 ttgaagtaat ctctctaaaa attgatatct atctaattta tttttatcat gtaattgtac
     4621 acttgttgct ctatgtctaa tactttcatc aaagttttct agttgttgcg tcaaattaat
     4681 acctatacca catattattg cttctatacc atcattatta gcaaccattt cagttaagaa
     4741 accacacact ttaccattat caatatatat atcattcggc catttcactt tgacttcatc
     4801 ttgactaaaa tgttgaatcg catctcttat ccctaatgca ataaataaat taaatttaga
     4861 tatcattgag aatgcaacgt taggtcttaa cacgacagac atccaaagtc cttgcccttt
     4921 tgaagaactc caatgtctat taaatcgccc acgacctttc gtttgttcat cactcaagat
     4981 aaaaaatgaa gattgatttc caacaagtga ctttttcgca gcaagttgtg tagaatctat
     5041 tgaatcgtat acttcactaa aatcaaacaa agcagaactt tttgtatatt ggtctattat
     5101 accttgatac caaatatctg ggagctgttg taataaatgc cctttatgat ttactgaatc
     5161 tattttacat ccctctaact ttaattggtc aatcactttt tttactgcag tgcgcgatat
     5221 attaagtgat tccgcaatgc tttgtccaga tatataattc ggtttatttt tatagagtaa
     5281 ttgaagtaca tcttgactat attttgacat gattatccac ccatttcaaa atttcagttt
     5341 cttcgttgct tactttacct gttacaatcg ctatctcaat ttgtcttagc acatctttta
     5401 accacggacc acttttggca tttaaatgtg ccataagtac accgccatta accatcatgt
     5461 cttttctatt atgcataggt aaacgatgta atgtttcatc aatcgtttga aggttaacga
     5521 ttaatggttc atgtccttga atatcatttg cctttatcac gtctgctgca accattacat
     5581 ttttaatgag atgcgtatca taatcataaa caaacatttt taattgttct tttgtaataa
     5641 tacttggtaa tgcattcata atttgaatat attgattgat atcttttact tgtcggttac
     5701 ttagctttaa aggcttcaat gagtaattaa tatcaaattt aactgatact atagcaatca
     5761 acaattctaa atcaattgct tcagttacat taatttgatt catatcaaga tgttcgaaat
     5821 acggcatata attaaatgct ttcagcgatt ttaaatgatt aaaactcttt tcaacattaa
     5881 tacctcgcat taatttagtt agttcaatca ctatacgctc aattgataaa aatttaatat
     5941 ctgccatttg tatacgcatc gcttcgaatg tttccgttgc aatatcaaat gataattgtg
     6001 actggaacct taaacatcga atcatacgta aagcatcttc ttggaatcgt tcctcagcta
     6061 tacctacagt tcttattatt cgattattaa tatcttgttg accatcaaaa taatcataca
     6121 atttgtatgc tgtatccatt gctatcgcat tcatcgtgaa atctcgtcgt tgcaaatctt
     6181 cgtataaatc acgaacaaat gtaacaccac ttggtctacg gtgatcgaca taatcttctt
     6241 cagcccggaa tgttgtcact tcataatttt catcattaaa aactacattt atcgtgccat
     6301 gttctttacc tacaggtatc gtatgactaa agatagattc tatttcatcc ggcgttgcac
     6361 ttgttgtgat atctatatca tgaatatttc ttcccatgac ataatctctt acagagccac
     6421 ctacataata tgcttcaaaa ccattgtctt gaatttgttc taatataggc cttgcctgtt
     6481 caaataatga tttatccata ttattactcg cctttacttt tgttatgctc atttagcatt
     6541 ttttgataat aatactcata ttgatctgta ataagttctg atccaaaacg ttcagcaata
     6601 tctgctagca tgtttttctg aagtttgttg tataacacct tatcttcaag taatcggata
     6661 gcatagtcac tcgctgaatc acaatcaccc acatctacga caaatccagt ttcaccatgt
     6721 ttaataacct ctttaattcc accggcattt gaaccaattg gaacgacgcc tgttttcata
     6781 gcctcaagta aagttagtcc aaagctttct ttttcactta ataataatac taagtcagat
     6841 aattggtaaa attcacttac gcaatcttgt ttccctaaaa ataaaacatc ctcttctacg
     6901 tttaactctt tcgtcaattg acgcattggc actaattcag gaccatctcc aagtaaaatt
     6961 aatttactag gtatcttttc acgtactttt gcaaatgttt ctataatagt atctatgcgt
     7021 tttacttgtc taaaattcga tacatgtatt aacacttttt catctggtgc tataccaaat
     7081 tgtgatttta atgctgtgtt atgtttagtt ggaaactcat tttcacgtac aaaattataa
     7141 atcggtataa tttctttgtt agtttcaata atttcatgtg tttcttgtgc taaagattta
     7201 ctcacacttg tcacaatatc acttttttca atgccaaatt taattgcacc ttggagtgaa
     7261 tgatcatagc ccaaaacagt aatatcagta ccgtgtagcg ttgtcataat ttttatatct
     7321 ttacctgaca tctcacgagc taaaatccca caaattgcat gaggtacagc atagtgcata
     7381 tgcaacaaat caagaccata ttctttaata acttcagcga ttttagtact taacgtaata
     7441 tcatacggtg gatactgaaa tactgcatat tgattcactt caacttgatg aaaaatcata
     7501 ttcggtaatg gttttcttat tctaaacggg atatttgaag tgataaaatg tacttcgtga
     7561 cctcgctctg ctaatttaat tcctaattct gtggcaataa ttccagaacc acccatggac
     7621 gggtaacatg ttatacctat cttcattcgc ttgcccatcc tttctttcta tttctctcta
     7681 ttattctcga tgcgtagata attgtttaaa tttaagttta tagtaatgtt gagtttataa
     7741 tttcaaatat ctaaaaaaca ggtgttgtat atatattcat ttatctagtt atatttactt
     7801 taaaaataat atgatttcat gcgatgcaat tcattgatgg atgtttttaa tcttaatcaa
     7861 atccaaataa agcatatatt tttatattca ctttctttcg aatcgatttt tatctcttgt
     7921 attaaacttt tccatagttt cattaaagct ctctgtcata tctattccca ttgaattcgc
     7981 taaacataac aacacaaata aattatcacc taattctgct ttaatcgtat ttgcttcctc
     8041 tgaatctttc ttcttttttt caccataggt atgatttatt tcacgtgcaa gttcgcccac
     8101 ttcttcagtc aatctagcta agttagctaa tggtgaaaaa tatcctgttt taaattgtcc
     8161 aatatattca tcaacttcac gttgcatttc taccattgat ttcatttcta cgttctcctt
     8221 atattgcatt tctaatatag tatatatcaa tttgaagtct catgcatgtt tagtgcaatc
     8281 tactgactct agctaaacta gatgacaagt tgtttatttt agaagtttaa taattcagtt
     8341 tatataaatg taatgcattc ctaactaaat taaatcaatt gaaaatggga ttataacttt
     8401 atgatacgta ctactacaat aaaataatat agtgaataat ctaccattag aaaaataagc
     8461 acaaaaaaac tagcaaccac acaaaatgtg attagctagt taataagtgt ctaatttaag
     8521 ttaattgtta atctataaga ttaatcactt gaacgcgcaa gcaaaatgat acgtacaagc
     8581 tctgctacag cgacagcagt tgctgcaaca taagtcattg ctgctgcaga taatacttta
     8641 cgcgcatgct tgtattcttt ttcatttaca atgttcaatg ccgtaatttg tttcatcgct
     8701 cttgaactcg catcaaactc aactggtaac gtaacaattg agaataatac cgctaatgac
     8761 attaaaccag caccaatcca taaagcagtt gaaccaaatg cactacctat cgctgttaag
     8821 ataataccta acatgatgat catataactt aatgaactcc ctaggtttgc aacaggtact
     8881 aatgctgctc tgaatcttaa gaaccaatat ccttggtgat cttgaatggc atgaccaact
     8941 tcgtgggctg caattgcagt tccagcaact gatggtctgt catagtttgc aggagatagt
     9001 gaaacaactt tctttttagg atcgtaatga tctgttaaga atccttcacc tttaacaact
     9061 tcgacatcat aaataccgtt tgcatgtaaa atttctaatg caacttcacg acccgtttta
     9121 ccactagttg atctaacttg tgaatatttc tcatagttag atttaacttt gtgttgtgcc
     9181 cataaaggaa gcaccattaa tattacgaaa taaattatca tagtaaaaat tgaagacaat
     9241 aaactcactc tcctttataa atattttact gtcatttgcc gtttttatca aatcatttac
     9301 actttaataa tttgtttaat tcaatataaa gcaaaagtcc aaaaacactc agacaacatg
     9361 ataatacacc aatttgccac acatgtgtag ttataaaatc ataatatgga aattgaaggt
     9421 gaaaatagtc aatataatca ttcaaaaata cccaagtcat cgctacactg attccaatca
     9481 tagaacgttt aaacctagga tagaagtaaa ttgcctgaac ggccattata ctgtgggaaa
     9541 acattaatat caggccattt actgtaatat caccttgttc aataataaat aatatattca
     9601 taataactgc ccaaatccca tatttgaata atgttacaaa tgccagtgca tcgataatac
     9661 tattttgttt ttggattaat atcattgaga tagaaataac taagaataat attgcagttg
     9721 ggctatctgg aacaaaaatc ttaaaatgcc acggcgtatg acttaattgt tcaccatacc
     9781 atatataacc ataaatcatc cctaatatat tacaaatgag tagcatcatt aaccaagaac
     9841 gttgataaag tgtatattgc caaaatgtat taatcgtcat ctgctaagtc ctcaaattga
     9901 ttatgtttat ttactagctt gagtgtattt aaaatttgcg ttagttgata aaaacgttgc
     9961 ttttcattca tctgtaaact taaatcaata ttgtgtaaca agtaatctat taataacgca
    10021 tgtttatgcc gatctatagc catactattt aagtcatgaa gataagtttg ataactgggc
    10081 gattcagtta gttgagcaac tattaaatca tctagccttt gctcacgctt tgacacgttt
    10141 gcgaagtgaa tttgaatatc aaaagcacag ttatgattag cgatataatc aaatatttca
    10201 tttgtattca ttaactttat attacgctta gtaaattgaa ttgcagaagc gtgacttccc
    10261 acttctgcaa tttctaatgt ttcatgatga ttaatttttg tatctacaaa atgaatgttt
    10321 gccaatttcg cctcattcac ttttatatag ttaagcaccc aaactgcaat acgcgactta
    10381 aatcgatatt gaaaaagtaa atattcaata aaactttctt taatttgatt gagtgtctct
    10441 gacatcaaat accccatttt aagattgcaa tcttgataat tcgtcatgcc aattttcgtt
    10501 acttggctct agttccaaca attgatttaa aatagtaatt gcttgttcct tttgaccaat
    10561 ttcagttaaa tagaaataat aatcactcat aaaatcaata tttgttttca tcgttggata
    10621 tgctaattca aagaaatgtt gagcttcttt atctcgctct tcttgaccat aggcgaacgc
    10681 taaatgccac atgaatgtag gatccaaatc ttcttcatct acatatgtta ataattcaat
    10741 gattgcttca taatcttctt cattacgata taaatcgctt aaaatcaata aaggttcttg
    10801 gtaagcatta tcaacctcta atgcttgtct taacaataat acaccttcat tagcatcgcc
    10861 gtgttctatt tccaaacatc cagttgtata cattaactct ttataaaatt gacttagtcg
    10921 taatccttct ttacccgtct caatggcatc tggataattt ttttcatttt catataatga
    10981 ttgtaaatac aagtagcctt gaatataatc aggatcttta gaaagtaatg tagtcattat
    11041 tttaattgct tcttgagtga tgtcattttt atcgtaagaa atggcttttt tgagataatc
    11101 ttctgaagtc atttcatctt cattaatttc atcgtataag cgaatcgcat cactatagtt
    11161 accactttgt aaactacaat ctgccatacg agagaataag tttacaccat taacttgata
    11221 ttcaccagtt tctaaaacgg tttcgtattc agaggtagca cgtaaatatt gaccatcata
    11281 atataacatt tcagccaatg caaaatggat tattggatca tttggttcta gttcaagtgc
    11341 ttcttgtaat ttagcaatag caacttccat catattaatt tgttgatata aatctgcttc
    11401 taacatcaac ttttcaggtg atggttcaac ataacttaaa tattctaacg cttcgtcagt
    11461 ttgattttca gacattaaac cttcaataaa ataaatcagc aattcacttt cgtctggata
    11521 tttgtgatat aacacgcgaa atacttccaa accttgtggc attaatccaa aattgtaaag
    11581 tgtctctcct agaataaata atgcgtcatc gttgtcagta gttattgctt cattaacacg
    11641 agagtctaaa ttttctagtt tttgtagatt gatatcgtct attaatttat agatatcttc
    11701 cattttatat tatccctcat tttctaaaag ctttagtttt ggtaaaaatc ctggaaatga
    11761 tacatttaca gcatcaaatt gtttgatttt gacaggctcg cttgaaagta gagaagcaac
    11821 tgcaagcatc attcctattc gatgatcagt taaactatca actgttgcat ttgttttaaa
    11881 ttctgacgga tgaataatca atccatcatt agttggttgt aattcaaacc ctaacaagtt
    11941 taacatatca gccgttgtat caattctatt tgtttctttt acttttaatt cctcggcatc
    12001 tttaattgta ctcgtgccaa ctgcttgtgt acaaagtaat gctattacag gcagttcatc
    12061 aattgctttt ggaactaatt ctccttcgat tgttattggt tgaagcattg gtgtgtattg
    12121 aatacgaata gaagcagtag gttcagcacc agttgtttga ttgaaaagtt ggatattacc
    12181 gcccattttt tcaacaatat caataatacc tgaacgtgtt ggattgattc caacattatg
    12241 aattgttaca tcacttcctg gtgtgataag tgctgcaaca ataaagaacg ctgcagatga
    12301 aatatcgcca ggaacatgaa aatctgcagg tttaatgtat cgaattgctt caggggttgt
    12361 attaattgat aacccttctg cttcaattga aatattaaaa tgtttgaaca tcgtctcagt
    12421 atgatttcga cttacatcta attctttaat gatggtcggt tccttagaaa acaaacttgc
    12481 aaataaaatg gcacttttta cttgtgcact tgcaacttcc atttgataat ttataccttt
    12541 tatgacagat ggcttaataa ttaatggtgt ataattatct tcaatacctt caatattcgc
    12601 atccataagt ttcaatggtc tcaagacacg atccattggc cttttaccaa ttgaaacatc
    12661 gccagacaaa acactttcaa tacctaaacc acttaacaaa cctgccaata atcgtgttgt
    12721 cgtaccagaa ttacctgtat acaatacttg atgtggcgtg ttaaaagatt gatatcctgg
    12781 ggaagtcaca actaattttt catcatcttc tttgatttct acacctaaca gtcggaaaat
    12841 gtccatcgta cgacgacaat cttcgccaag tagtggctta tatatagtag atacaccttc
    12901 agctagcgac gccaacatga ttgcacggtg tgtcattgac ttatcgcccg gcacttctat
    12961 ttcgcccttt aacggacctg aaatatcaat gatttgttca tttaccattt cattcaccta
    13021 cttaaaatat gtttttaatt gttcacatgc atgttgtaat gttagttgat caacatgttg
    13081 tacaacgata tctccaaatt gtctaatcaa gaccatttgt acaccttgct tatcattctt
    13141 tttatcactt agcatatatt cgtataacgt ttcaaaatcc aagtcagtta tcatgtctaa
    13201 aggataaccg agttgtatta aatattgaat ataatgatta atttcatgct tagaatcaaa
    13261 caaagcattc gcaactataa attgatagat aatgccaacc atcactgcat gaccatgagg
    13321 tattttatga tagtattcaa cagcatgccc aaatgtatga cctaaattta aaaatttacg
    13381 tacaccttgt tctttttcat ctgcaacaac aatatccagc ttcgtttcaa tacctttagc
    13441 aatatattta tccataccat ttaatgactg taatatctct ctatctttaa agtgctgttc
    13501 gatttcttgc gtcgttgatt caccattcaa taacgcatgc ttataaactt ctgcatagcc
    13561 acttaatatt tgctcaaatg gtaacgtctt taaaaagtct aaatcataaa tcacagcagt
    13621 tggacgataa aatgcaccga taaggttttt accttgtttt gagttaatac ccactttacc
    13681 gccaacacta gaatcatgcg ccaaaatcgt tgtaggaact tgtataaaat ggacacctct
    13741 taatagtgtt gctgctacaa atcctgcaaa atcaccagtt gcaccaccac caacagcaat
    13801 aattgctgta ttacgagtca catgatggga taaaatatac tctaatgttt cttgatattg
    13861 atgaaatgtt tttgtctttt cacctgctgg aataatgact ttatgcacat tttcatatga
    13921 taaaatatca tcaaatttat tcgcaaaata ttgatttaca tattcatcaa ttaatataaa
    13981 actttgatca aactgatcaa tatacgtgct aatatggtca attgcaccgc gttcaacata
    14041 tattggataa ttatttgaag ggtatgttgt ttgtaatttc atgattacac ctcaattgtt
    14101 cttgttgtta aaactcaata tttaattgtc tgcgctcaat aatttgttgt ttaagttgct
    14161 caatatgatt tgattggaat tcttccaata atgctttcgc aatttcaaat gctacgacat
    14221 gttcgcagac gatacttgct gcaggaacag cacaactatc agaacgttca attgttgctt
    14281 taaagtcttc tttagtatta atgtctactg aatttaatgg tttatataac gttggaattg
    14341 gtttcattac accattaacg ataattggca ttccatttga cataccgcct tctaaaccac
    14401 ctaagtgatt tgacccacga taatagccca attcagtatt gtagagaatt tcgtcttgaa
    14461 tttcgctacc aggcttttca gctgctttaa atccttctcc aaaacttaca cctttaaatg
    14521 cattaatact aacgacaccc tgtgctattc ttccatctaa tttacgatca taatgtacat
    14581 aactacctac accaacaggc atattttcaa ctacaacttg aactactccc cctattgaat
    14641 caccatcgtt tttagcttcg tcaattttat ctcgcattgc ttgtgcgata ctgtcatcaa
    14701 ttacacgaac atcattacga tcaagatttg ctttaaatgt ttttgaatca taaaaatctt
    14761 tatctttaat tccacctatt tcaacaacac gactgtatat atcgatatct aactgttgta
    14821 ataacacttt acataaggca ccgactgcaa ctcgagctgc tgtttctcta gcagatgatc
    14881 gctctagcac atttcgtaaa tcacgatgat tatatttcat acctccaacc aaatctgcat
    14941 gaccaggtct tggttttgta atagtacgtt tcatattttc acgttcttct tcacttattg
    15001 gagctgctcc cataattttt ctccaatgcg taaagtcatc attggttaca accatagtaa
    15061 ttggactacc taatgtataa ccatttctaa cgcctgatac tatttctact gtatctttct
    15121 caatttgcat gcgtcggcca cgaccataac caccttgacg cttgaacatt tctttattaa
    15181 tatcttcaac tttaatttct aaatttgctg gtataccttc gacaataact gttaattgag
    15241 gtccatgtga ttctccagaa gttagatatc tcatacacac tcgctccttt catacttccg
    15301 caataacatt ataatatgtt gttaatctta tcacattcat tgtggattca taaatgtttg
    15361 ttttcaagct taattaagaa aatggatgac atctcatttt aaatgtcaga attatcttaa
    15421 tattaaacga actaaaatta attataatat attcgtctgt agaaaagtat catgaatttc
    15481 taaacagttg catcatataa tcattaatta aaaatgatta gcc
//
LOCUS       DBNENW010000051        15517 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNENW010000051 DBNENW010000000
VERSION     DBNENW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.051"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            117..845
                     /locus_tag="AC1LW8_001307"
     CDS             117..845
                     /locus_tag="AC1LW8_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HIP4773151.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
     gene            complement(1257..1618)
                     /gene="ssrA"
                     /locus_tag="AC1LW8_001308"
     tmRNA           complement(1257..1618)
                     /gene="ssrA"
                     /locus_tag="AC1LW8_001308"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1709..2173)
                     /gene="smpB"
                     /locus_tag="AC1LW8_001309"
     CDS             complement(1709..2173)
                     /gene="smpB"
                     /locus_tag="AC1LW8_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIP4773152.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(2195..4567)
                     /gene="rnr"
                     /locus_tag="AC1LW8_001310"
     CDS             complement(2195..4567)
                     /gene="rnr"
                     /locus_tag="AC1LW8_001310"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIP4773153.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRNLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDINEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGSQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(4601..5341)
                     /locus_tag="AC1LW8_001311"
     CDS             complement(4601..5341)
                     /locus_tag="AC1LW8_001311"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4773154.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQAENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(5457..5690)
                     /gene="secG"
                     /locus_tag="AC1LW8_001312"
     CDS             complement(5457..5690)
                     /gene="secG"
                     /locus_tag="AC1LW8_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIP4773155.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(5757..6215)
                     /locus_tag="AC1LW8_001313"
     CDS             complement(5757..6215)
                     /locus_tag="AC1LW8_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773156.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(6554..7858)
                     /gene="eno"
                     /locus_tag="AC1LW8_001314"
     CDS             complement(6554..7858)
                     /gene="eno"
                     /locus_tag="AC1LW8_001314"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HIP4773157.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILVKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            complement(7988..9505)
                     /gene="gpmI"
                     /locus_tag="AC1LW8_001315"
     CDS             complement(7988..9505)
                     /gene="gpmI"
                     /locus_tag="AC1LW8_001315"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIP4773158.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            complement(9508..10269)
                     /gene="tpiA"
                     /locus_tag="AC1LW8_001316"
     CDS             complement(9508..10269)
                     /gene="tpiA"
                     /locus_tag="AC1LW8_001316"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIP4773159.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            complement(10391..11581)
                     /locus_tag="AC1LW8_001317"
     CDS             complement(10391..11581)
                     /locus_tag="AC1LW8_001317"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIP4773160.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            complement(11720..12730)
                     /gene="gap"
                     /locus_tag="AC1LW8_001318"
     CDS             complement(11720..12730)
                     /gene="gap"
                     /locus_tag="AC1LW8_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIP4773161.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            complement(12783..13796)
                     /locus_tag="AC1LW8_001319"
     CDS             complement(12783..13796)
                     /locus_tag="AC1LW8_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIP4773162.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            complement(14438..15067)
                     /locus_tag="AC1LW8_001320"
     CDS             complement(14438..15067)
                     /locus_tag="AC1LW8_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HIP4773163.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKPDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT         4789 a         2973 c         2300 g         5455 t
ORIGIN      
        1 attatttaat atcatcttag aatctcacaa aatatcaact ttgtttaatt actcaaaatg
       61 ataagtcatt tagagttttt aaaataaatt tttgcgaaat aaaggagaca tgttaaatga
      121 aaaaagtaat ggggatatta ttagcaagta cacttatctt aggtgcttgt ggacatcatc
      181 aagatagtgc aaaaaaagag agcactagtc acaaaaagaa agaaaatgac aatgaagaat
      241 taaatgaaga acttaaagaa tttaaaagca aaaaaaatat ggatataaaa attaaaggcg
      301 atactattgt tagtgacaaa tttgaagcta aaataaaaga accgtttatc atcaatgaaa
      361 aagatgagaa aaagaaatat atcgctttta aaatggaaat tactgctaaa aaagacgata
      421 aagatttaaa tccatcttct atttctcatg actatattaa tatcactcaa gatgataaaa
      481 atacagtaaa taaattaaga gatggttatc ttttaagtga taaaaaatat aaagattgga
      541 cagaacataa ccaagatcaa attaaaaaag gcaaaactgc acaagccatg tttatctatg
      601 agttaagagg tgatggaaat attaatttaa atgtccataa atactcagaa gataaaacag
      661 ttgattctaa atcattcaaa tttagtaaac ttaaaaccga ggatttttct catagagcgg
      721 aaacaagaga agaagtagaa aagaaagaaa aagaatttga agaagagtac aaaaaagaac
      781 aagaacgaga gaaagaaaaa gaaaagcaaa aagatgacga ccacagtagt ttagatgaag
      841 tataaattta tgaatgctcg catacagcgt tataatcttt gtaattatta ttgtcattat
      901 aaattagctt acctacactc ataatgacac actaatttaa ttgttaaata caacaaaaaa
      961 gagtagctcg cctactcttt tttgttgttt tgaatgaaga attatcttat ataaagaatt
     1021 ttttatgaac aatattaaaa ctctaagctc aacttttttg acacttattt gacacatatt
     1081 ctttcaaccc ttaacgcaaa atcttatatg tacacaagta ttaatttttc attcatgaaa
     1141 atttgaacct aagcaaatac tatcatatca acgtacacag ctaatttaac cttcctcaca
     1201 agaatagata aaaaacatat aaaaaagccc acatccacaa aggttgtagg ctacaaatat
     1261 ggagacggcg ggatttgaac ccgcgtccag aggtcctgat acacatcttt ctacgtgtgt
     1321 agtttatcga aaggtttcgc atcatgaaaa gtgataaaca accaacatga tgctagcttg
     1381 attaagtttc ttctaaacag acttcaaacg gcagtgttta gcatatccta ttaaggttga
     1441 atcgcgttaa cagcatatag gaaatgctgt taggcgatgc agagtgcgat taggcagcta
     1501 ctgcgaaatt attgtttgat ttgccagtta ttataaactg tgtgtgttga tgacgaagac
     1561 aaccctccga cacgcttaat gagctcgggg gacccctgtc gaatccatga acatccccga
     1621 aataataaat caaacaaatc atacttttgt aatttacaaa agcacatata actatatcaa
     1681 atatagcccg attaagcaac taaatcgctt aataacgggc tttcatatcg cgcgcaacat
     1741 ctcgtttgac tgctttttct ttcaaagctt gacgtttatc atattttttc ttacctcgtg
     1801 cgacaccaag taatacttta caatgcccat gcttcaaata aagctttaat ggcacaatcg
     1861 aataaccaat ttcacgtgtt tgatcaccca atttaatgat ttcacgttta tgcaataata
     1921 attttcgaga acgaagagga tcgtgattaa aacgattccc ttcttcgtat ggtgctatat
     1981 gcatattatt caaatacatt tcaccgtttt taacttgcgc gtaactatct ttaaggttag
     2041 cactacctcg gcgaatcgat tttatttctg taccttgtaa tacgattccc gcttcaatcg
     2101 tgtcttcaat attataatca tgtcttgctt tacgattttc cgctaatgta cctggtgatt
     2161 tcttcttagc cattcatact cacctcattg ctgcttattt tttcttacga cgtgctttct
     2221 ttttcacact tttatcttta taaaatggct tatgtttact atttcctgat ttgtcatttt
     2281 tgcgttcatt tttaccttta cgttgactac cctttttctt acgtccttta tcatcagatt
     2341 ttgatttatc taatgattta ccacgcgttt tggcttgaat tgtcttacct cgcgctgggc
     2401 gctgtgatcg atcatttttc ggtaaaggca tacctacaat ttgaaaatca attaatcgtt
     2461 catctacatc aacatgcgtc actttaacct taactgtgtc accaattcta aatactttag
     2521 cttgacgctc accaattaat gccatttgac gctcttcaaa acggtaataa tcatcagtca
     2581 tattcgcaat atgaaccata ccttctatcg tatttggcaa ttcaatgaac ataccgaagt
     2641 tagctactga gctgacaata ccttcaaatt catcaccaat atgttgaatc atatattctg
     2701 cttttttcaa ttcatcagta tcacgttctg cctcaatagc acgacgttca cgtttagaag
     2761 tatgttcagc taactcaggc aatttgtctt cccaacgctt cacttctttg ttatccattg
     2821 atttctcaat taaatactta cggattaaac gatgaactgt taaatcagga taacgtctaa
     2881 ttggtgatgt aaaatgcgta taatattcag ctgataagcc aaaatgtccc aagttcacat
     2941 catcataatg cgcttgttgc attgaacgca acatcattgt tgaaatgacc atttgttcag
     3001 gtcgaccttc tacttcttct tgaacctttt gaagtgttgt tggatgaata tcttcgccag
     3061 tacccttaat catgatgcca aagtttgtaa taaaatcaaa gaattgtctt aagcgatctg
     3121 atttaggttg ctcatgcact cggtaaataa aaggtacatc taacttacta aaatgttcag
     3181 caactgtttc atttgcaatt aacataaatg attcaattag acgttcaccc tcgccacgtt
     3241 gtcttaattg aacatctgtt ggtataccgt cttcgttaac taatactttt gcttcattaa
     3301 tatcaaaatc gatttcacca cgtcgttttc tcatttgaat caaacgatta gataaatctt
     3361 gtgctaaatc taacataggc gtaatttctt tatattgttc gcgaatgtta ggatcctttt
     3421 cagtaataat ctgatttacc gcatcatacg tcattcgata atcagaatgt ataacactat
     3481 caaaaatttc atgtttaaca acgcgaccac tagcatcgat ttccatgcga cagcttagag
     3541 ttaaacgatc aacattagga ttcaatgaac aaataccatt acttaatcga tgtggaatca
     3601 ttggaattac acggtcaaca agatatacac ttgtcgctct atcatatgcc tctttatcca
     3661 atgcagaacc ttctgttaca taatagctga catcagcaat acttacagtt aattgcgtat
     3721 taccgttcgc taacttttta acactaattg cgtcatctaa gtctttagca tcagcaccat
     3781 caattgtgat tgtcaattca tcacgtaaat catgacggcc tttaatttca gtattttcaa
     3841 tatgatcagg tactgcttca gcttcttgta acacttcatc aggaaattca atttctatgc
     3901 catgttgata gataatagat aaaatatcta cgccaggatc atttttatgt cctaaaatag
     3961 cagaaatatg tccttctgga ttatctgaac catcagcata tttagtaatt tgaacaagta
     4021 ccttatgccc atctactgca cctaaacttt gtcctttagg aatgaaaata tcttgcataa
     4081 tacgtttatc atctggaata acaaatccga aatgtctagc ttcactatac gtaccaacaa
     4141 cttgagttac tgaatgcttc tcaatcgact taacttcccc ttcaatttta cctttatgtt
     4201 cacctttaga ttgatgtatt tctacaatta cagtatctcc atctaaggca cgattaattt
     4261 ttgttggggg aataaatata tcttccatat cctcatcttc aggtcttaag aatgcaaagc
     4321 cttttttatt ttgacttaac gttcccttta tcaattttga atgacctttt gaactatgct
     4381 ttttttgata tctgtctgtt tttgtacgtt caattaatcc tgattgttct aattctacga
     4441 gcaccttaat taaatttcta aatgagtcgg cactacttaa acctaatgca tcttgaaaat
     4501 ctgacactga cataggttca tattcaggtt gattaataat ctcttctatt gattgcttta
     4561 aattcattat gcccctcctt tctaatgtta aaatctcttt ttattctgac cagtctaatg
     4621 actctaaaaa ttgataaata tcttcaaata cttgttcttt ttctttatca atcgtaataa
     4681 catgtcctga ttgactatac cacttgatgt ctttgtcatc agaatctaca tggtcatata
     4741 tataatttgc ggattgtgga tcaatcatat tgtcgttttc tgcttgaatc actaaaattg
     4801 ggtcaaatac ctcatcaact tgatctttaa tcgtatctaa tgcttcactt agttctttta
     4861 aagtttcagt tggtttaaaa tgatccattt cattatcaat agtctcttga tttttacctt
     4921 catacttttt aaaattacgt gcatactcta aaaagccttc ataaatggca ccttcagttt
     4981 tgccacccat tggtgcacac atcgttacaa taccctttac atctctattt aagcttaatt
     5041 ttaaagcaaa atccccacct aatgatagac cagcaacaac aatttcatca taaccttgtt
     5101 caacaagata atcataacca tctaacgcat ctttaaacca aacgaaagga ctagatttca
     5161 gtatttcctc tggtggtgcc gcgtgacctt catattgtgg tgcatacgaa gtatatccct
     5221 ttttttgtaa aaatcgacct aattgacgta catcagacga attgcctgta aatccatgta
     5281 ataataacac ggcacgtttg ccttcttcaa aaaagaaagg ttttggtaat tttatctgca
     5341 taaatctaac gctccctttg catacttaac taggctatat attttacata gcttattgtt
     5401 aaactattac attcttaatt ataaataaaa aagcccgaca tttacatcgc cggaccttac
     5461 ataccaagat aacttatgca aatcataagt acaaaaaata atattgataa aataattgtt
     5521 aatctattta agaataaatc gacgccacgt tgtttttgtt taccgaataa ctgctcagca
     5581 ccaccactga tggcacctga aagtccactg cttttacctt cttggagtag tacaacagtt
     5641 attaatgcaa tacaatcaat gattaataat acgattaaaa atgtatgcat aaattgtcct
     5701 ccgttcctta tatacgacaa gtagtatatc atatttttat tgttaaaatc aattttctag
     5761 cgattaaatg tacttttacg tttgatagaa acaacaagca tataaatcgc taatgcaacc
     5821 gaacctataa taacgattaa aattggcaat gttaagtacg caccgtgtgc agcttttatt
     5881 gcttgaattg tgattgataa attaatcaac gtaaacaaac tatttgatac ataaactgca
     5941 aacataaacc accacacata agtatgtcga ttccaaaaag tgatgaagcc tgctagataa
     6001 gccaaaatat acataatacc agtgaatcta aagctgttta aaatgtaatc aatcgcttta
     6061 tctgaagtgt tttgattcgt taaattaagt aatgctttgg cagtcgcatt atccaacgaa
     6121 acaaagtaat gaataatcaa taatattgag aatacgattg atgcagttgc aataaagcgt
     6181 cctgttttaa tttctttatt cgttctatca gccatttgtt tactcctcat atatttaaat
     6241 acgttctata aaacctttat ttaattttac cttttaataa aagtgaatac cctgtcatac
     6301 aatattcatt tcaacataaa tttattatac cagtttaaga atacaagtta aatatgtgtt
     6361 attagttacc tcatgattat tagtctgtta tgaataatac ttcaaaataa aaaacctacc
     6421 acagtctcaa ctctgtggca ggttataaat acaagcgtga actttttaag cgtgaactca
     6481 aaaactttat tcaatttata ccgacataat aatcctcaac taaaattatg gcagtactta
     6541 atgtttaaaa tgattattta tctaagttat agaatgattt gataccgtca tatttagcag
     6601 tttcaaataa ttcatcttcg atacgtaata attgattgta tttagcaata cggtcagtac
     6661 gtgataatga accagtttta atttgaccag cgtttgtagc aacagcaata tcagcaattg
     6721 ttgtatcttc tgtttcacct gaacggtgag aaactactgc tgtgtaacca gctttttgag
     6781 ccatttcgat tgcatcaaat gtttcagtta atgtaccgat ttggttaact ttaattaaga
     6841 ttgagttacc aattccgttt tcaatacctt ttactaaaat ttcagtgttt gttacgaata
     6901 aatcgtcacc tactaattgt acacggtcac cgatacgttc tgtaagttgt ttccaaccat
     6961 cccagtcgtt ttcgtccata ccgtcttcaa ttgtaatgat aggatatttg tctactaatt
     7021 gttctaagta gtcaacttgt tctgcagctg tacgttttgc accgtgttcg ccttcgaact
     7081 tactgtagtc atatacacca ttttcataga attctgatga tgcacagtca aatcctaaga
     7141 atacttcttc acctggtttg taaccagctg cttcgattgc ttggataatt gtttcaacag
     7201 catcttcagt accttcaaat ttaggagcga aaccaccttc gtcacctact gcagtttcta
     7261 aaccacgttt gcttaaaatt gattttaagt tgtggaaaat ttcagtaccc caacgtaatg
     7321 attctttgaa cgttgtagca cctacaggta aaatcatgaa ttcttggaat gcaattggag
     7381 catctgagtg agaaccacca ttaacgatgt tcatcattgg tactggtaac tgcttaccat
     7441 taaatccacc taaatatttg taaagtggtt gacctaataa gtcagctgct gcacgtgcta
     7501 ctgcgataga tacacctaaa atagcatttg cacctaattt acctttgttt ggagtaccgt
     7561 ctaatgcgat catcatttta tcaatagata cttgatctaa tactgaaaat tcaccttcaa
     7621 taatttctgg tgcgatgatt tcattaacgt tttcaactgc tttagtaaca cctttaccta
     7681 aataacgtga tttgtctcca tcacgtaatt caacagcttc gtgttcacca gttgaagcac
     7741 ctgatggtac taatgcacga ccaaatgcgc cactttcagt taatacttct acttcaacag
     7801 ttgggttacc acgagagtct aagacttcgc gagcgtaaac atctgtaata attggcatgt
     7861 ttataatctc ctttaaatag ttattctata attattatag ctttattctt tcatttaaca
     7921 aaatgatatt actattattt tcaaaaaata agtgtcatta agcgtttact taacatcttt
     7981 tacaatatta gtgtttaatt aaagattcac ctgtcatgtc ttcaggttgt tctacattta
     8041 ataaatctaa taatgtaggt gctaagtcac ctaagcgacc agtttctcga agtgtaacgc
     8101 cttcttttgt tacaatcact ggtactgggt tcgttgtatg cgtagtcatt ggttgatcat
     8161 catccgtcaa tacttgatca gagttaccat ggtcagcagt aataattgca taaccgtcca
     8221 tgtctaaaat cttatcaacg acttcaccta aacattcatc aaccgcttcg attgctttga
     8281 ttgtcggctc aagcatacca ctatgtccaa ccatatcagg gttagcaaag tttaaaataa
     8341 ttaagtccaa gtcaccttta tttaactctt ctaataatgc atctttaact tcataagcac
     8401 tcatttctgg tttcaagtca tacgttgcaa ctttaggtga atcaattaaa cgacggcgtt
     8461 cacctttaaa ttcctcgtta cgtccaccac tcataaagta agtaacgtga ggatattttt
     8521 cagtttctgc aatacgtaat tgtgttaaat tgttattttg tgcaatttca ccaattgtat
     8581 tatttaaatc aactttttcg aaaacgatag ccgcatcgat attgtcgtta tacttagtga
     8641 atgttgcata gaataagtct ttaacttgtt caactttaaa gccttcgaat gctctgttcg
     8701 caaaaatttc cgataattgt gctgctctat caggtcggaa attatagaag atcactgcat
     8761 ctccatcatt aacaccgtca ttttgattct caacgatgaa tggtactacg aattcgtcag
     8821 ttaagccctc attatagctt gcttctacac cttctttggc agttgcataa gttggggcat
     8881 caaaattacg aatagcattg taagcttttt cttcacgttc ccaacgtttg tcacggtcca
     8941 ttgcataata acgaccagac acagatgcaa attgaccaat gcctaattca ttgaatttag
     9001 cttcagtctc ttcgatgtat ttcaaagcgg atttttgatc tacgtcacga ccatctaaaa
     9061 atgcgtgtac gtaaactttt tcaacacctt gttttttagc aagttctaac aaagcaaata
     9121 aatgtttgta atgactgtgc acaccaccgt cagacaataa accaaagatg tgtaacgctg
     9181 aatcatgtga attcacgtgt gcaattgcat tatttaaaac atcattttca aagaaatcac
     9241 cgtcttcaat tgatttattg attcgagtta aactttgata aacgatacgt cctgcaccga
     9301 tattcatatg accaacttct gagttaccca tttgtccttc aggtagtcca acatctaagc
     9361 cactcgcttc gatttgagtc gttggatatt tgttgtaata acgatcaaaa ttaggcttgt
     9421 ttgctaattt taccgcatta ccatgttcgc tttcgcggtt cgcaaaacca tctaaaataa
     9481 ttaacgcagt tggtttctta gccatgatta ttttgcacct tctaacaatt gtacgaaatc
     9541 ttcaacttta agtgatgcgc cacctactaa tgccccatca atatcagttt gtgccatgta
     9601 ttctttaatg ttgttaggtt taacactacc accatattga atacgagttg cttctgatac
     9661 ttctttgctt gataagtcag caatagtttg acgtacaaat gcacacattt catttgcatc
     9721 ttcagatgtt gatgatttac cagttccgat tgcccagatt ggctcataag caattacaac
     9781 tgatttaagt tgatcttcag ataaacctgc aacagctttc ttaacttgct cacctacaac
     9841 atcgttagct ttaccacttt cacgctcttc gtctgtttca ccaacacaaa taattggagt
     9901 cattccatgt ttgaaaatag cgtgcgcttt tttgttaatt tcttcatctg tttcgtggaa
     9961 taattcacga cgttcagaat gaccgataac aacgtattta acgcctaaat ctgctaatgc
    10021 aactggagac gtttcacctg tgaacgcacc attatcttcg aaatacgtat tttgagcacc
    10081 gatttctaaa ccttgtgctt ttccttcttt aactgcagta gttaatgcat ctaattgaat
    10141 tgctggtgca caaattactg attctacttc ttttgaatct ggtaatgttg gtaatgcatt
    10201 gacgaagtct tttgcttctt gtactgtttt gttcattttc cagttaccag ctataattgg
    10261 tgttctcatt aaagacactc cttgctttgt aaatattttt gaaaagtgat gaaacacgat
    10321 gtcatcttgt gactgttttc ccgtaacaat gttaaacaaa catgccacat cactttaaac
    10381 tatcacttta ttatttatta ttgattgctt tgataccagg caattcttta ccttctaggt
    10441 actctaatga cgcgccgcca ccagttgaaa tatgagtgaa gtcattttca aaacctaaag
    10501 agattgctgc tgcagctgaa tcaccgccac cgataatcgt aattgcatct ttaaggtttg
    10561 caattgcttt acatacacca attgtacctt gtgcaaagtt actgaactcg aatacaccca
    10621 taggtccatt ccatacaaca gtgtgcgcac cttctaattc atctgcaaat aattttacag
    10681 tgtttggtcc aatatccata ccttcttggt ctgctggaat tgaatcagat ggtactacag
    10741 tgattttggc atcattagaa aattctttag caactttagt gtctactggt aatacaattt
    10801 tatcaccatg tttttctaat aaatcttttg cgaagtcgat tttatcttct tctaataatg
    10861 aaataccaat ttctttacct tgcgctttta agaaagtata agccatacct ccgccgatga
    10921 taattttatc agctatgtta actaagtttt tgatgacatt aattttgtca gatacttttg
    10981 ctccacctaa aatagcaaca actggtttat gtggatcgtt aactacgcca ccaataaact
    11041 taatttcttt atccattaag aatccagctg cagtttctaa atgtgtagaa ataccaacat
    11101 tagatgcatg ctcacgatgc gcagtaccaa aagcatcatt tacaaacaca tcacctaaag
    11161 atgcccagta tttacctaat tctggatcat ttttagattc ttttttaccg tctaaatctt
    11221 cataacgtgt attttcaact aataatacgt cgccttcttt aaggtcttta atagcagctt
    11281 caagtttttc gccgcgtgtt tctggtacga aaacaacttc tttatctaat ttcttagata
    11341 agtcttcagc aactggacgt aaagttaatt ttgctttatc actttcttct ttcactttac
    11401 ctaaatgtga aaatagtacg attttaccac cttgttcgat gatgtattga attgtaggta
    11461 aagcttgaac gatacggttg tcattagtaa tttcaccgtc ttttaaaggt acgttaaaat
    11521 cagcacgtac taggactgtt ttacctttaa gatctaaatc agaaacaatt tttttagcca
    11581 tggtgtttcc tccttgtaat taggaaattt tatataaaaa taagcggaga agcgtttgtg
    11641 ctccccgctt actcataagc tttaattaat gctaaatagt tctgagcact agcgtatttg
    11701 aataaaaact atactaaaat tatttagaaa gttcagctaa gtatgctaat gtacgaacta
    11761 attgtgcagt atatgacatt tcgttatcat accaagctgc aactttaact aattgacggt
    11821 cgccaactga cattacacga gtttgtgtag cgtcgaataa tgaaccgtaa gtcataccta
    11881 caacgtctga agaaacgatt tcgtcttcag tgtaaccgaa tgattcgttt gaagcatttt
    11941 tcatagcttc gttaacttgt tcaactgtta cgtcttgttt ttctaatact actgttaatt
    12001 cagttaatga acctgtagct acaggaacac gttgtgcacc accatctaat ttaccatcga
    12061 tttcaggaat aactttaccg atagctttag cagcacctgt tgagttaggg atgatgtttt
    12121 ctgccgctgc acgagcacga cgtttgtcac cttttctgtg aggtgcgtct tgtgtatttt
    12181 gatcacctgt gtaagcgtga attgtagtca ttaaaccttc aactaaacca aagtcatcgt
    12241 ttaaaacttt agcaactggt gctaatgagt ttgtagtaca tgaagcacct gaaacaactg
    12301 tttcagaacc gtctaactct tggtggttag tgttgaatac gattgttttt aagtcaccag
    12361 tagctggtgc tgagattaat acttttttag cgcctgcttc aatatgagct tgtgctttat
    12421 ctttatcagt gtagaaacca gtacattcta acactacatc gatatttaag tctttccaag
    12481 gtaatttgct tgcatctggt tcactgaatg atttaacttc tttaccattt acgcggaaac
    12541 caccatcaac tacctctact tcacctgtga aacgaccttg catagtgtca tattttaata
    12601 aatgcgctaa catgtcgtca tctgttaagt cgtttactgc tacaacttca agaccttcta
    12661 cttcttgaat tcttctgaat gctaaacgac caattctacc aaaaccatta attgctactt
    12721 ttactgccat tataatggcc tcctttaaaa tgatatttaa aaagtattaa actttttatc
    12781 tcttattcaa gtattatctt tgctgcggct tcatcagtga ttaacactgt attcttgggt
    12841 gcaatcgtca agtatgcttt aattgcttca cctttcgatt tgcctcctgc aactgcaaaa
    12901 ataaagtctt ttgattcaag gtcttctaat tgaagtccaa ttgttttaac cttatggaca
    12961 atttgacctt gtgtatcaaa ataataacca aatgcctctc cgacagcttg atgatgttga
    13021 agttgttcaa tgaccttttc aggtgattga cgtcgatgcg ccatcttcag cgcatcacca
    13081 atgccgtgta atataacgtt tgcttgttta attttgtcta aagtgtttat gactgatggc
    13141 tctaacaaca atgtgttata tgttgtttca ctgacattat caggtacata catcgtcgta
    13201 taataaccgc cagcttgttg tgccatactg gctgcaattg tgtttgcctg aaagacaaca
    13261 ttttcgccta gtccacctct ggctggtacg aagaatacat tatatggtaa taaatgaatt
    13321 gcttcactaa cacatgccat cgtggatccg ccagttacag caactatcgc gtcttcttgt
    13381 aatatgcctt caagtaattg acctgcttgt ctacctaatt ctgttttaac agattgacta
    13441 ttatcagcat caccaggaac aacatgaact tccttaattt gaaatttatt cttaatacct
    13501 tctgacagac gattatcatc tgcatagata tcaaagtaac ctttcaattg cgaaatcagt
    13561 tgctcacctt cagctgtaat ttccattccg gtaggcttaa cttttatcaa atcttgtttc
    13621 ttaagcatat ctgtttcaga acgcagtaca cgttcagtca tatccatatg ttcgcttaaa
    13681 cttcgacgtc cgacaggctg attttttgag atagtagtaa gaatagaaaa acgtttatac
    13741 attttatcta tgagatccgg tataagcttt tgctgtgctt gcaataagtc tttcactact
    13801 acctcctcct tatatttata aatgtaaata aggatatgtc acaaccagtg gttgcctttt
    13861 taagtcccgc gtgggacaaa aattaacctc tttacacttg caatagtact attctatttc
    13921 aaaaattgca agcataaaac ttaaattttc taaataattt gataaagcgc ttgcaatata
    13981 ttttcgattt gttattcaac aaaacgtttt attttattcg ctgtagtcaa atgtataccc
    14041 ttatactgac tcgctaatca tttgcttatt atttgaacta agacataaat caatgctcta
    14101 gaatccgaaa agtcacatta gtagtagttg accgaacgaa aatgcacttg taacaagcat
    14161 ttttcaatta aagtcagggg ccccaacaca gaagctgacg gaaagtcagc ttacaataat
    14221 gtggaagttg gggtcagacg acgaaataaa ttttgcgaaa atatcatttc tgtcccacct
    14281 cactcaaaat aaatttcact ttccgtaaaa taaacaacta actatttaag tatcatctct
    14341 cccctaatta acaattataa aaataagaac caatattcga gacaacctaa ttaattgtgt
    14401 tgtctgtcat actggttcat ttttgtttga ctaaatacta cttatcatta gcattatttt
    14461 gtttatttgt tgttgattgg ctattttgtt gtggtgcttg tggttttgct tgctgatttg
    14521 attgattatt ttgttgatta ttatttggct ttggtgttgg ttgttgcggc tgtggtttag
    14581 gctgagctgg ttgtttatta tcatcagttt tcttctgatt atcatctggt ttatcttgag
    14641 atgcattgtc atccttatct ttcgactcat cctttgatgc tttatcatca ttctcttctt
    14701 tagcaggacg ttgctttgta ggtgcttgtt gctgaggtgg cacatattga tattgattct
    14761 gctgtggcaa ttgctgatat tggtttgctt gtgaatctag ctcattctgc tctttttcct
    14821 cttgttgctt tttctttctc tctttttctg cattttcttt ttgacgcttt tctttttgtt
    14881 cctgcttttg ttcgttcaac atacgttctt tagctttatt cgaatgatct acatatgcaa
    14941 aaattgcaaa tactaatcct ccgagtaata gcaataccgt cgcaatgcta actactttcg
    15001 ctgttgtact cattttcttc ttacttccat cttcattttt attgtagtcg taattttgat
    15061 tcgacatact ctccctcact tcaaactaat ttaaatatag aaattatcac gcaaccatta
    15121 acattttcac aactaatttg cttacctagt ttctacaaat attaataaac atttactctt
    15181 tttgccttct tcaacttaaa actgccttca ctattactca ctcgtaaatc ggattcattt
    15241 ttacaaagaa atagtagtct tatatatctt aacatttaat aactaaatca aacatatttt
    15301 gagccttttt ttgaaaatta acattttaac ctttttgatt ttacaacaaa ttatagctac
    15361 gtattgaaaa ttaaagcatt ggtttaagtg ttgtgttaaa agtttttatg tttagattgt
    15421 tatattacta tgtattctta aatttgttta aaaaattaat gctttaaatt gatgtataat
    15481 gggaataaga aataaataaa aacgacccgc acgatta
//
LOCUS       DBNENW010000052        15458 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNENW010000052 DBNENW010000000
VERSION     DBNENW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.052"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            75..2597
                     /gene="mprF"
                     /locus_tag="AC1LW8_001321"
     CDS             75..2597
                     /gene="mprF"
                     /locus_tag="AC1LW8_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIP4773164.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSEIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2800..3309)
                     /gene="msrA"
                     /locus_tag="AC1LW8_001322"
     CDS             complement(2800..3309)
                     /gene="msrA"
                     /locus_tag="AC1LW8_001322"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIP4773165.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3446..4429
                     /locus_tag="AC1LW8_001323"
     CDS             3446..4429
                     /locus_tag="AC1LW8_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP4773166.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4517..4702)
                     /locus_tag="AC1LW8_001324"
     CDS             complement(4517..4702)
                     /locus_tag="AC1LW8_001324"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HIP4773167.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4850..6112
                     /locus_tag="AC1LW8_001325"
     CDS             4850..6112
                     /locus_tag="AC1LW8_001325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIP4773168.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6248..7339)
                     /locus_tag="AC1LW8_001326"
     CDS             complement(6248..7339)
                     /locus_tag="AC1LW8_001326"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIP4773169.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7504..8535
                     /locus_tag="AC1LW8_001327"
     CDS             7504..8535
                     /locus_tag="AC1LW8_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIP4773170.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAIILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9027..10433
                     /locus_tag="AC1LW8_001328"
     CDS             9027..10433
                     /locus_tag="AC1LW8_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIP4773171.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQISERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10430..10996
                     /locus_tag="AC1LW8_001329"
     CDS             10430..10996
                     /locus_tag="AC1LW8_001329"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIP4773172.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            11002..12000
                     /gene="trpD"
                     /locus_tag="AC1LW8_001330"
     CDS             11002..12000
                     /gene="trpD"
                     /locus_tag="AC1LW8_001330"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIP4773173.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            12002..12784
                     /gene="trpC"
                     /locus_tag="AC1LW8_001331"
     CDS             12002..12784
                     /gene="trpC"
                     /locus_tag="AC1LW8_001331"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIP4773174.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12784..13416
                     /locus_tag="AC1LW8_001332"
     CDS             12784..13416
                     /locus_tag="AC1LW8_001332"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIP4773175.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13409..14623
                     /gene="trpB"
                     /locus_tag="AC1LW8_001333"
     CDS             13409..14623
                     /gene="trpB"
                     /locus_tag="AC1LW8_001333"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIP4773176.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            14616..15344
                     /gene="trpA"
                     /locus_tag="AC1LW8_001334"
     CDS             14616..15344
                     /gene="trpA"
                     /locus_tag="AC1LW8_001334"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIP4773177.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
BASE COUNT         5418 a         2245 c         2842 g         4953 t
ORIGIN      
        1 atgatatgct atttttatgg tttatatgat atattttgat ttataacaga aataattaga
       61 attgatgtga aaaaatgaat caggaagtta aaaacaaaat attttcaatc ttaaaaatta
      121 cgtttgctac agctttattt atttttgtag taatcacatt gtatcgggag ttatctggta
      181 ttaactttaa agatacgttg gttgaattta gtaagattaa ccgtatgtcc ttagttttac
      241 tatttattgg tggtggggca tcgcttgtta ttctatcaat gtatgatgtg attttatcta
      301 gagctttaaa aatggatata tccttaggca aagttttaag agtaagttat atcatcaatg
      361 cattgaatgc gattgtaggt ttcggtggct ttattggtgc aggcgttaga gctatggttt
      421 ataaaaacta tacgcatgat aaaaagaaat tagttcactt tatatcctta atacttattt
      481 caatgttgac aggtttaagc ttattatcat tgttaattgt attccatgtt ttcgatgcat
      541 ctttaatttt agataagatt acatgggtaa gatgggtatt atatgtagtg tcatttttct
      601 taccattatt cattatttat tcaatggtta gaccacctga taaaaacaat cgttttgtag
      661 gattgtactg cactttagtg tcgtgtgttg aatggttagc agctgcagtt gtattatatt
      721 tctgtggtgt aattgttgac gctcatgtat cattcatgtc ctttattgca atatttatca
      781 ttgctgcatt atcaggttta gtcagcttta ttcctggtgg tttcggcgct ttcgatttag
      841 ttgtattact aggatttaaa actttaggtg tccctgagga aaaagtatta ttaatgctac
      901 ttctatatcg ttttgcgtac tattttgtac cggtaattat tgcattaatt ttatcatcat
      961 ttgaatttgg tacatcagct aagaagtaca ttgagggatc taaatacttt attcctgcta
     1021 aagatgttac gtcattttta atgtcttatc aaaaggatat tattgctaaa attccatcat
     1081 tatcattagc aattttagta ttctttacaa gtatgatctt ttttgtaaat aacttaacga
     1141 ttgtttacga tgctttatat gatggaaatc acttaacgta ttatattcta ttggcaattc
     1201 atactagtgc ttgtttatta cttttactga atgtagttgg tatttataag caaagtagac
     1261 gtgccatcat ctttgctatg atttcaattt tattaatcac agtggcgaca ttcttcactt
     1321 acgcttcata tattttaata acatggttag ctattatttt tgttctgctt attgtagctt
     1381 tccgtagagc acgtaggttg aaacgcccag taagaatgag aaatatagtt gcaatgcttt
     1441 tattcagttt atttatttta tatgttaacc atatatttat tgctggaacg ttatatgcat
     1501 tagatattta tacgattgaa atgcatacat ctgtattgcg ctattacttc tggcttacga
     1561 ttttaatcat cgctatcatc ataggtatga ttgcatggtt gtttgattat caatttagca
     1621 aagtacgcat ttcttctgaa attgaagatt gcgaggagat tattaatcag tatggcggta
     1681 attatttgag tcacttgata tatagtggtg acaagcagtt tttcactaat gaaaataaaa
     1741 cagcattttt aatgtatcgt tataaagcaa gttcattagt ggttcttgga gatccgttag
     1801 gtgatgaaaa tgcctttgat gaattgttag aagcattcta taattacgct gagtatttag
     1861 gctatgatgt tatattctat caagttacag atcaacacat gcctttatat cataatttcg
     1921 gtaaccaatt tttcaaatta ggtgaagaag caattattga tttaacgcaa ttttcaactt
     1981 caggtaaaaa acgccgtgga tttagagcga ctttaaacaa attcgatgaa cttaatattt
     2041 cattcgaaat tattgaacca ccgttttcaa ctgaatttat aaatgaactt caacatgtaa
     2101 gtgatttatg gctagataat cgtcaggaaa tgcatttctc tgtgggtcaa tttaatgaag
     2161 aatacttatc taaagcgcca attggtgtaa tgcgaaatga agaaaatgaa gtaattgcat
     2221 tttgtagttt aatgccaaca tactttaatg atgccatttc agtcgattta attagatggt
     2281 tgccagagtt agatttacca ttaatggatg gtctatactt gcatatgtta ctttggagta
     2341 aagaacaagg ttatacaaaa tttaatatgg gtatggcaac gttatcgaac gttggtcaat
     2401 tgcattattc atatttaaga gaacgacttg caggccgtgt ctttgaacat ttcaacggtc
     2461 tatatcgttt ccaaggatta cgtcgttata aatctaaata taatccgaat tgggaaccac
     2521 gctttttagt ttatcgtaaa gataattcgc tttgggaatc actttctaaa gtaatgcgtg
     2581 taatacgtca caaataatta aaatccaagt gctaagaggt atacagttat gcctgaagcg
     2641 cttggatttt ttggtttcta taaagaatga tttataaaaa acggtacaga taagaatatt
     2701 taagtttcct atctgtaccg tattttaata ttgaaatgag cttaagttaa gtcgcctaac
     2761 ttttattatc agtccaatca gtttcatcaa gtataaatgt tattgcttat ttttgtattc
     2821 ttggcgtatt ttttgttctt ctgcatagcg ctctggattt ttcttataaa aatcttggtg
     2881 atagtcttcg gctttgtaaa attgtgacgc gggtaatatt tttgttgcaa ttgccttatc
     2941 agcattaatc gtatttttaa gctgctcgat ataagtctca gcgagttctt tttgatgatc
     3001 attagtgtag aaaatagctg tttgatattg aggaccacgg tcttgatatt gaccccctgt
     3061 atctaatggg tcaatgactg agaaaaatat ttctaataac ttattgtatg agaataatgc
     3121 aacatcatat tgaatttcaa cagtttctaa atgaccactc gtacctgatt ttacttgttc
     3181 gtaagtagga ttttcaatat gtccgcccat atatccagaa gttacttttt ctatgccgtc
     3241 aaaggtgtca aatggtttcg tcatacacca aaagcaacct ccggcaaaat aagctgtatt
     3301 aatattcatt tttgacatcc tttcattaga ccttagtacg atttattaag aaatcacttg
     3361 ctttttgaat tgtttttata taacgttaat atgtgattat tatagtacac aaaaatctat
     3421 ttttgaagat ataaggtagg taaatatgga taaagaaact aatgacaacg aatatagacg
     3481 tcaaagtgaa catcgcactt cggcgcctaa gcgaaaaaag aagaagaaaa ttaggaaatt
     3541 acctatcatt cttctgattg ttgtaatttt acttatcgca ttagttgtat atattgtgca
     3601 tagttacaat agcggtgtag aatatgccaa gaaacatgcg aaagatgtta aagtacatca
     3661 atttaatgga ccagtaaaaa atgatggtaa aatttctatt cttgtactcg gtgcagataa
     3721 agcacaaggt ggacaatcaa gaacagattc tatcatggtt gttcaatatg actttatcaa
     3781 taaaaagatg aaaatgatgt ctgtcatgcg tgatatttat gcagatattc caggatatgg
     3841 aaaacacaaa attaattcag catacgcttt aggtggtcca gagctactta gaaaaacact
     3901 tgataaaaat ttaggaatta atcctgaata ttatgcagta gttgatttta ctggatttga
     3961 gaaaatgatt gatgaattaa tgccagaagg tgtaccaatt aatgtcgaaa aggatatgtc
     4021 gaaaaatatt ggtgtatctt tgaaaaaggg taaccatagg ttgaatggta aagaattact
     4081 tggttatgca agattccgtc acgaccctga aggtgacttc ggacgcgtgc gacgtcagca
     4141 acaagtgatg caaacattga aaaaagaaat ggttaatttt agaacagttg ttaaattacc
     4201 aaaagttgca ggtattttaa gaggctatgt gaatacaaac attcctgatt cagggatttt
     4261 ccaaacaggt ttgagttttg gtatccgagg tgaaaaagat gttaagtcat tgactgtgcc
     4321 aatcaagaac tcatacgaag atgtcaatac aaatactgat ggtagtgcat tacagattaa
     4381 taaaaacaca aataaacaag ctattaaaga ctttttagat gaagattaaa aataaacaag
     4441 gcgatttcta tcatactcag atagaagtcg ccttgttcgt tttcaaagaa ttaattggca
     4501 tgcatcttat gaagaattat tgatctgact ttcttacgcc agccacacca taatggtttg
     4561 gtttcatttc ttctataaca acgtgaattg cttgtctatt tgcccccgtt gttttttcta
     4621 cggcgtcagt tacttcgcta actaaatttt ttaattgttc atccgaacga ccttctaata
     4681 attttacatt gacgattggc atcatccaaa cctcctaatc attattaatt tgagtatatc
     4741 atgtaatact taaaaattca tttgcaaaat aacagaacgt ttgttctaaa atagttgaaa
     4801 tggaacgcgt gttcgtatat aatgagattc aagtaaggga ggaataacag tgtataatta
     4861 tcatttatta gaagataggg atgttctatg tattgaccaa aaaagttttt ttgcgagtgt
     4921 ttcttgtatt gaaaaggggc tagatccatt agaaacaaag ctagctgttg ttgcagatac
     4981 taagcgtcag ggttctgtaa tattggctgc gacacctaaa ttaaaagaat taggcatcaa
     5041 gacagggtcg cgattgtttg aaataccaca tagaaatgat atttacatta tcaatccaag
     5101 tatgcgtaaa tatcttaatg tttcagttgc tatttctaag attgcattgc gttatattcc
     5161 acctgaagat ttacaccaat atagtattga cgaatttttt atggatgtta ctgatagcta
     5221 tcatagattt agttctacag tacatgcatt ttgcgaaaga cttaaacgtg aaatttatga
     5281 agaaacaggc atttattgta ctgtgggcat tggttctaat atgttattaa gtaaaattgc
     5341 tatggatgtt gaagcgaagc atagtcaaaa tggtatagct gaatggcgat atcaagatgt
     5401 accaacgaaa ttatggccaa ttcagccctt gcgagatttt tggggtatta atcgtcgaac
     5461 agaagccaaa ttgaataaaa gaggaatttt tactatagga gatttagcga aatatccata
     5521 taaattttta aaaaaagagt tcggtatttt aggtgttgat atgcatctac atgcgaatgg
     5581 gatagatcag agtaaagtac gtgaaaagca caagatcagc aatccatcga tatgcaaaag
     5641 tcaaatatta atgagagatt atcattttga tgaagcaaaa gtagtaatgc aagagttaat
     5701 tgaagatgtt gctagcagag ttcgagcaag aaaaaaagtg gcaagaacga tacattttgc
     5761 ctttggctat agtgatgaag gcggtgtaca taagcaatat actttgaaag atccaacaaa
     5821 cttagaaaaa gatatttata aagtagtaat gcatttcgca gataaattat gtaataaaca
     5881 agcactatat cgtacgctaa gtatatcttt gagtcaattt attaatgagg atgagcgaca
     5941 gttaagtctg tttgaagatg aataccaacg caaacgtgac gaatgtctag ctaaaacgat
     6001 agaccaatta catttgaaat acggcaaagg tattgtgtcc aaagcagtat cgtttacaga
     6061 agcaggtaca aaacacggca gattaggttt aatggctgga cataaaatgt aatgactata
     6121 cggtttaagt aatatataac tgtgattcgt ataaaataag tctctaaaga taaaaatttc
     6181 atatatcaca atagatttca caataatatc taagaataca tggaatttat caaaagagac
     6241 ttaataatta ttggatataa caatcaaaat cactcaatgc ttgcataccg cgttctcggt
     6301 cagtagggtt tttgaaacta atttttaaag caccgtatat atcttcgcgt acttctaaga
     6361 ttcttaagtt gcttatagat atgttatgta aactcaggat ataagtcact ttacttatca
     6421 tacctgattc atccggaatg tctacatata gatcatacgc agtatttagt ccacctagtt
     6481 gtttagcggg tagtgcgtcg cgatacgatt tagcttgggc aaaaaatgat aacaattttt
     6541 cagaatcatt gctttcaatt agtctttcta aatcttgaaa ctgacttttt agctgtcgaa
     6601 tcatttctaa aatatacgtt ttattactca aggtgatatc tttccacatt tgtgcattac
     6661 tactagctat acgagtgata tcacgaaaac caccagctgc aagtttatta actaaatgat
     6721 gttcttgacc gttcttttga ctaacatgaa ctaaactaga tgcaacgata tgaggtaaat
     6781 gacttacgac gcttgttacg tagtcgtgtt cttcagcagt agttacaata aatttagcaa
     6841 gagtaggtga taacagttct tttaacgtgt ttgctgcttg ctcatttctt ggctcattgt
     6901 agactaaaat ataataagcg ttttcaaata agtgcttttt agcatttagt acaccagatt
     6961 tatgactacc agccattgga tgaccactga ctaaatgaat attatgcttt aataaattgc
     7021 attcgtgttg ctgtatcatt gctttagtac taccagtatc agaaacaata acaccaggtt
     7081 tagttggcat atctataagc tcgctaagat atttatttgt gatagcaaca ggtgttgcat
     7141 aaataattac atcggctttt ttaatagctt cactataatt taaacatttt tcattaataa
     7201 tgccgattga tttagcttta tctaactgag aagtatctgc atcgtatgca ataatattag
     7261 tattagggtt atggtatttt atattgctag caagacttcc accaattaat ccaagcccaa
     7321 caaataaaac tgttgtcata taaatcacct tatttcgaaa ttttcagaat aataacattg
     7381 taaatgagct gttgacacag tgcaatagta aataaaaatc gataatagca ttaatagata
     7441 aacggagata aatcatctac aataaagagt atagtaacac aatggcaacg gaggggtaaa
     7501 tcaatggaac caatattaga aatgattaaa acattaacag gtattaatag tccttcagga
     7561 gacacagaag aagcaattca atttgtcgaa aaatatgcaa aagacttggg ttatcaaaca
     7621 acactaacaa ataaaggtgc gttattaata acagtgccag gcaaaaatga tgaagtacaa
     7681 cgctgtatta ctgctcatgt tgatacttta ggtgcaatgg ttaaagaaat taaagaagat
     7741 ggtcgcttag caatagaatt aattggagga ttcacgtata acgcgattga gggtgaatat
     7801 tgccaaatta aaactgatgc tggtcaaata tatacaggaa caatttgtct gcatgaaaca
     7861 agtgttcatg tatatagaaa taatcatgaa atacctagag atcaaaagca tatggaaata
     7921 agaattgatg aagtaactac atcagaagaa gatacaaaga gtttaggtat ttcagtaggt
     7981 gattttgtta gctttgatcc acgtacagtt atcacgtcat caggttttat taaatctcgt
     8041 catttagatg ataaagctag cgtagcgatt atactacaat tactaaagaa attaaaagaa
     8101 gagcaaataa tattaccaca tacaacgcaa ttttatattt ctaataacga agaaataggt
     8161 tacggtgcaa atgcatcaat tgattcgaaa atcaaagaat atattgcatt agatatgggc
     8221 gcgttgggag acggtcaagc atcggatgaa tatacagttt ctatttgtgc caaagatgct
     8281 tcaggtccat atcataagca attgaaatcg cacctagtta atctttgcaa aataaataac
     8341 attccatata aagtcgacat atatccatat tatggttcag atgcttcagc agctttacat
     8401 gctggtgcgg atatcagaca tggtttattt ggcgctggca ttgaatcatc tcatgcaatg
     8461 gaacgaacac atattgattc tattaaagcg acagagaaat tactatatgc atattgctta
     8521 tcaccaattg agtaaacaat tagtgttgac aaatgtgaac gacctatgta atataatgaa
     8581 ctataaaaat aattagaatt ttctaaagaa atagtagcag atatgaaacg tagcaaatag
     8641 aaagctaatg ggtgatggga attagcacgc catatcttgt gaattggact ttggaaaaca
     8701 attgaatgag ttttgaaagt gaacatgaat tatgttaact aaggtggcac cacggtaacg
     8761 cgtccttaca ggtatatgcg ttatgtggtg tctttttatt tagacaaaat gtagtagtta
     8821 attaaaggta gcaacagaaa gttagtggat gatgtgaact aacaccgaga ttaatgaaat
     8881 tgggttttgt ctgcaacaga aaaattatat atagtaaaga gtgaactatg aatatttcga
     8941 atattcggtt aatttaggtg gtaccacgcg tcagcgtcct ttatattgat aaggatgctg
     9001 gcgctttttt gaaaggagcg tatagaatgg atatatttta taaaaaaata aaagcaaatg
     9061 taacgccaga agttttagca caacttcatt ccaagaagat cattttggaa agtacaaatc
     9121 aacaacaaac taaaggtcgc tattcagttg ttatttttga tatttatggc actttaactt
     9181 tagataatga tgtattatca gtaagcactt taaaagaatc gtatcaaatc agtgaaagac
     9241 cgtaccatta tttaacgact aaaataaatg aagactacca taatattcaa gatgagcaac
     9301 ttaagtcatt accgtttata tctggatatg ttgggacgtg tagctttgat ttagtaagac
     9361 atgaatttcc taaattgcaa tcaatacaat tagaagatca caagcagcac gatgtaaggt
     9421 tatatatggt tgaacaagtt tatgtatttg accattacaa agatgagtta tatatcatcg
     9481 cgacgaatca attttcaaat tcaacaaaat cagatcttga gaatcgagtt aataagtcta
     9541 tcgaagactt aactaaaatc caaccattca tgcctacaca agattttgat tttaaaacta
     9601 aagaaattca atcaaacatt tctgaagaaa gatttatcga aatgattcag tatttcaaag
     9661 agaaaataac agaaggggat atgtttcaag ttgtgccatc aagaatttac aaatatgcgc
     9721 atcatgctag tcagcattta aatcaacttt cgtttcaact gtatcaaaat ttaaaacgac
     9781 aaaacccaag tccatatatg tattatctta atatcgatca accatatatt gtcggtagtt
     9841 ctcccgaaag tttcgtaagt gtcaaagatc aaattgtaac aactaatcct attgcaggta
     9901 cgattcaacg tggtgagacg acacaaatag ataatgagaa tatgaaacaa ctacttaatg
     9961 atccaaaaga atgcagcgaa catcgtatgc tagttgattt agggcgtaat gatattcata
    10021 gagtaagtaa aatcggtacc tcaaaaatta ctaaattaat ggttattgaa aaatatgaac
    10081 atgttatgca tatcgtaagt gaagtcacag gtaaaataaa tcaaaattta tcgccaatga
    10141 cagttattgc gaatttatta ccaacaggta ccgtttcagg tgcaccaaaa ttacgtgcaa
    10201 ttgaaagaat atatgaacaa tatccacata aacggggcgt ttatagtggt ggtgttggat
    10261 acataaattg taatcataac ttagattttg cattagcaat tcgaacgatg atgatagatg
    10321 agcagtatat caacgtagaa gctggttgtg gcgttgtata tgattctatt cctgaaaaag
    10381 aactgaatga aacgaaattg aaagctaaaa gcttattgga ggtgagccca tgatcttagt
    10441 tgtagataat tatgattcct ttacatataa cctagtggat attgttgctc aacatactga
    10501 cgtcattgtt caataccctg atgatgataa tgtgctgaat caatcggtgg acgctgttat
    10561 tatatctcct ggtccagggc atccattaga cgatcaacag ttgatgaaaa tcatatcaac
    10621 ctatcaacac aaacccattt taggtatatg tttaggggct caggcactga cttgttacta
    10681 cggtggagaa gtcattaaag gcgacaaggt tatgcacggc aaagttgata cactaaaggt
    10741 tatatcgcat catcaacatc tgttatatca agatatacca gaacagtttt caattatgag
    10801 atatcattca ttaataagta accctgacaa ttttccagaa gaattgaaaa ttactggacg
    10861 taccaaagat tgtatacagt cattcgagca taaagaaaga ccgcattatg gtattcagta
    10921 ccatcctgaa tcatttgcta cagactatgg tgtcaaaata attacaaatt tcattaatct
    10981 agtgaaggaa ggatgaaaac catgacatta ctaacaagaa taaaaactga aactatatta
    11041 cttgaaagcg acattaaaga gctaatcgat atacttattt ctcctagtat tggaactgat
    11101 attaaatatg aattacttag ttcctattcg gagcgagaaa tccaacaaca agaattaaca
    11161 tatattgtac gtagcttaat taatacaatg tatccacatc aaccatgtta tgaaggggct
    11221 atgtgtgtgt gcggcacagg tggtgacaag tcaaatagtt tcaacatttc aacgactgtt
    11281 gcttttgttg tagcaagtgc tggcgtaaaa gttataaaac atggtaataa aagtattacc
    11341 tcaaattcag gtagtacgga tttgttaaat caaatgaaca tacaaacaac aactgttgat
    11401 gatacaccta accaattaaa tgaaaaagac cttgtattca ttggtgcaac tgaatcatat
    11461 ccaatcatga agtatatgca accagttaga aaaatgattg gaaagcctac aatattaaac
    11521 cttgtgggtc cattaattaa tccatatcac ttaacgtatc aaatggtagg cgtctttgat
    11581 cctacaaagt taaagttagt tgctaaaacg attaaagatt taggtagaaa acgtgcaatc
    11641 gttttacatg gtgcaaatgg tatggatgaa gcaacactat ctggtgataa tttgatatat
    11701 gaattgactg aagatggaga aatcaaaaat tacacattaa atgcgactga ttatggtttg
    11761 aaacatgcgc cgaatagtga ttttaaaggc ggttcacctg aagaaaattt agcaatctcc
    11821 cttaatatct tgaatggtaa agatcagtca agtcgacgtg atgttgtctt actaaatgcg
    11881 ggtttaagcc tttatgttgc agagaaagtg gataccatcg cagaaggcat agaacttgca
    11941 actacattga ttgataatgg tgaagcattg aaaaaatacc atcaaatgag aggtgaataa
    12001 tatgacgatt ttatcagaaa ttgttaaata taaacagtca cttttacaaa atggctatta
    12061 tcaagacaaa cttaatacct tgaaaagtgt gaagattcag aataaaaaat cttttataaa
    12121 cgcaattgag aaagaaccaa agctagcaat tattgcagaa attaaatcga agagtcctac
    12181 agttaatgac ttacctgaac gagatttatc gcaacaaatc tcagattatg accaatatgg
    12241 tgcaaatgcc gtgtccattt taactgatga aaagtacttt ggtggtagtt ttgaaagatt
    12301 acaagcattg acgacaaaaa caacattacc cgtattatgc aaagacttta ttatagaccc
    12361 gcttcaaatt gatgttgcta aacaagctgg tgcatctatg attttattga tcgttaacat
    12421 cttatctgat aaacaattga aagatttata taactacgct atatcgcaaa atctagaagt
    12481 gttagttgaa gtacatgatc gccatgaatt agaacgtgcc tataaggtta atgctaaatt
    12541 gattggtgta aataacaggg acttaaaacg atttgttaca aatgtggaac atacaaatac
    12601 tattttagaa aataaaaaaa caaatcatta ttatatttct gaaagtggta ttcacgatgc
    12661 atctgatgta agaaaaatct tgcatagtgg tatcgatggc ttactaatag gtgaggcgct
    12721 tatgcgttgt gacaatctat ctgaattttt accacaactg aaaatgcaaa aggtgaagtc
    12781 atgatgaaat tgaaattttg tggctttaca tcaataaagg atgttacagc ggccagtcaa
    12841 ttacctattg atgcgatagg tttcatccat tatgaaaaaa gtaaaaggca tcaaacaatt
    12901 acccaaataa aaaagttagc gtctgctgtt ccaaatcata tcgataaagt atgtgtcatg
    12961 gtaaatcctg atttaacaac aattgaacac gtattaagca atacgtcaat taacacaata
    13021 cagttacacg gcacagaatc tattgatttt atacaggaaa ttaaaaagaa atattcaagc
    13081 attaaaatca ctaaagcttt agctgcagat gaaaacataa tccaaaacat aaataaatat
    13141 aaagggttcg tagatttatt tattatcgac acaccctcag tgtcgtatgg tggtaccggt
    13201 caaacatatg actggactat tttgaagcac ataaaagaca taccttattt gatagcagga
    13261 ggcattaact ctgaaaatat tcaaacagtt aatcaactta aattatcaca tcaaggttat
    13321 gatcttgcat caggtataga agtaaatggg cgaaaagata tagaaaaaat gacagcaatt
    13381 gtaaatattg tgaaaggaga tagagacaat gaataaacaa atacaaacag aagcagatga
    13441 attaggtttc tttggtgaat atggagggca atatgttcca gaaacattaa tgccagcaat
    13501 tattgagttg aaaaaagctt ataaagaggc aaaagcagac ccagagtttc aaagagaact
    13561 ggaatactat ttatcagagt atgtaggacg cgcgacacca cttacatatg ctgcatcata
    13621 tactgaaagc ctaggtggcg ctaaaatata tttgaaacga gaggatctaa atcatacagg
    13681 cgcccataaa attaataatg cgttaggtca agcgttgctt gctaaaagaa tgggcaagaa
    13741 gaagcttgtt gctgaaactg gtgcgggtca acatggtgta gctagtgcta cggttgctgc
    13801 attatttgat atggaacttg ttgtctttat gggaagtgaa gatattaaaa gacaacaact
    13861 taatgtattt agaatggaat tacttggtgc aaaggttgtg gcagttgaag atggtcaagg
    13921 gactttatcg gatgcagtta ataaagcatt gcaatattgg gtaagtcatg tagatgatac
    13981 acattattta ttaggttctg cattaggtcc agacccgttc ccaacgattg ttagagattt
    14041 tcagagtgtg attggtaaag aaataaaatc acagatattg aagaaagaag gtcgacttcc
    14101 ggatgcaatt gtagcatgta tcggtggtgg ctcaaatgca atcggtacat tttatccatt
    14161 tattaaagat gatgttgcat tatacggtgt tgaagccgca ggtcaaggcg atgatactga
    14221 taaacatgca cttgcaattg gcaaaggatc acctggcgta ttacatggta ctaaaatgta
    14281 tttaattcaa gatgaagatg ggcaagtgca actagcacat tctatttcag caggacttga
    14341 ttatcctggt attggaccag aacattctta ttaccacgac attggtagag taacttttga
    14401 aaatgctagt gatacacaag caatgaatgc tttaatcaac tttacaaaac atgaaggtat
    14461 tatacctgca attgaaagtg cacatgcact gagttatgtt gaaagactag cgcctacgat
    14521 gtcgaaagaa gatattattg tagtaactat ttctggacgt ggcgataaag atatggaaac
    14581 aattagacaa tatatggtag agcgaggtct tgcaaatgac taaattattt ataccttata
    14641 ttatgggcaa taaagatttg attgaaaatg caacattgtt gagtgaaaat ggtgcagata
    14701 taattgaaat tggagtacct ttctctgatc cggttgctga tggtccagtt atcatggaag
    14761 caggtcaaca agcgattaaa caaggcatca cgatagatta tattttcaat caattagaaa
    14821 aacatggtga tcaaattaag tgtaactatg tattaatgac gtattataat attatttgtc
    14881 attatggaga acaagcgttt tttgaaaaat gtcgagatac tggtgtctac ggcttaatta
    14941 ttcctgattt accatatgaa ttatcgcagc gtttaaaaca acaatttagt cactatggcg
    15001 tcaaaatcat atcgttagtt gcgatgacta ctgatgacaa acgtataaaa gatatcgtat
    15061 cccatgcgga aggctttatt tatactgtga cgatgaatgc gacaacaggg caaaacggtg
    15121 cgtttcatcc agaattaaaa cgaaaaattg agtcaattaa agcgatagcc aatgtgccag
    15181 ttgtcgcagg atttggtata agaacaccac aacatgttgc agatataaaa gaggttgcag
    15241 atggcattgt cattggtagc gaaatcgtta agcgatttaa atctaacacg cgtgaggaaa
    15301 tcattaaata tttacaatct atccaacaaa cattgaataa ttaagtttac ttgatttaaa
    15361 aaaattaggc gaatactgtt tgaaaaagtg aaaaacggtg aattataaaa ttgaatagaa
    15421 atgatacaaa agtaatatga gcaaacccaa acgttcat
//
LOCUS       DBNENW010000053        15409 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNENW010000053 DBNENW010000000
VERSION     DBNENW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.053"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(285..773)
                     /locus_tag="AC1LW8_001335"
     CDS             complement(285..773)
                     /locus_tag="AC1LW8_001335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773178.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(1049..1750)
                     /gene="isaA"
                     /locus_tag="AC1LW8_001336"
     CDS             complement(1049..1750)
                     /gene="isaA"
                     /locus_tag="AC1LW8_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HIP4773179.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(2358..3413)
                     /locus_tag="AC1LW8_001337"
     CDS             complement(2358..3413)
                     /locus_tag="AC1LW8_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIP4773180.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGISGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(3531..3668)
                     /locus_tag="AC1LW8_001338"
     CDS             complement(3531..3668)
                     /locus_tag="AC1LW8_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773181.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYRCY
                     C"
     gene            3823..4392
                     /locus_tag="AC1LW8_001339"
     CDS             3823..4392
                     /locus_tag="AC1LW8_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP4773182.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            4590..5090
                     /locus_tag="AC1LW8_001340"
     CDS             4590..5090
                     /locus_tag="AC1LW8_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773183.1"
                     /translation="MTSIFVFAYLNNTSDNMLFRQQMICACLLANVQHSSIVCLNNTC
                     CIVDIRCLNTKIGGIDVSETDEPQGFERTHNILNINQNRQSLMTFIISKLKSTLKQRI
                     INARGKKRIDYRLSYNFYITYYDTVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            5310..5540
                     /locus_tag="AC1LW8_001341"
     CDS             5310..5540
                     /locus_tag="AC1LW8_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIP4773184.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(5703..6080)
                     /locus_tag="AC1LW8_001342"
     CDS             complement(5703..6080)
                     /locus_tag="AC1LW8_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4773185.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(6097..6918)
                     /locus_tag="AC1LW8_001343"
     CDS             complement(6097..6918)
                     /locus_tag="AC1LW8_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HIP4773186.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(6938..7237)
                     /locus_tag="AC1LW8_001344"
     CDS             complement(6938..7237)
                     /locus_tag="AC1LW8_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HIP4773187.1"
                     /translation="MSLNKEQRRITCEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            7369..7926
                     /locus_tag="AC1LW8_001345"
     CDS             7369..7926
                     /locus_tag="AC1LW8_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP4773188.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            7919..8623
                     /locus_tag="AC1LW8_001346"
     CDS             7919..8623
                     /locus_tag="AC1LW8_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP4773189.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            8721..9731
                     /locus_tag="AC1LW8_001347"
     CDS             8721..9731
                     /locus_tag="AC1LW8_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIP4773190.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSA
                     NN"
     gene            9751..10569
                     /locus_tag="AC1LW8_001348"
     CDS             9751..10569
                     /locus_tag="AC1LW8_001348"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4773191.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     IVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGMKTFG
                     ETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKYSDKI
                     TLLNGTDSRGSFPQDVNFYINKETSIPIVDIPGGHLGYIQKPEGFADVLLNMWG"
     gene            complement(10716..11606)
                     /locus_tag="AC1LW8_001349"
     CDS             complement(10716..11606)
                     /locus_tag="AC1LW8_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIP4773192.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPAKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(11703..13043)
                     /locus_tag="AC1LW8_001350"
     CDS             complement(11703..13043)
                     /locus_tag="AC1LW8_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIP4773193.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(13037..14143)
                     /locus_tag="AC1LW8_001351"
     CDS             complement(13037..14143)
                     /locus_tag="AC1LW8_001351"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP4773194.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(14438..14734)
                     /locus_tag="AC1LW8_001352"
     CDS             complement(14438..14734)
                     /locus_tag="AC1LW8_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HIP4773195.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(14722..15090)
                     /locus_tag="AC1LW8_001353"
     CDS             complement(14722..15090)
                     /locus_tag="AC1LW8_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773196.1"
                     /translation="MSNKLDEINKIITAKHKQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(15105..15383)
                     /locus_tag="AC1LW8_001354"
     CDS             complement(15105..15383)
                     /locus_tag="AC1LW8_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HIP4773197.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
BASE COUNT         5051 a         2794 c         2376 g         5188 t
ORIGIN      
        1 tttagcaaaa taactgttat tttcattata tttttacaaa aaaaggttcg ttttatattt
       61 tatgcctctt actgtaacag aatcattaag atatgctatt cgaatatact ttttcaaaat
      121 ttatataatg aataaattaa catgtattga aaaaaagcga aatgcagcct atcctctaat
      181 gtaaaccaaa cgatatatct cgtcagactt tatatttaaa cgctatgtgt cacttttaaa
      241 atgaatatta ctaagattgt catatcaatt attattgcat cgaattaatc ttttaaattt
      301 ctgtaataaa acctattatc tacatctaat ttaggaaaac tacttggtaa atctcctcgc
      361 ttaatttcac ggaagccatt attagaataa aaatactgtg cactaataaa tttatcaatt
      421 gttcctaaat aaataccatc gatattttgt tctttacatg tcattataac tttatctaaa
      481 agttttttac ctatttttaa attcctataa cctttatcaa caaacatttt tttaagtgca
      541 gacatattat tatctagtct aatcaaacct atagtaccaa caatattttg atgattgttt
      601 attgcaagcc aaaattgccc tccattattc aaatagttat gttcgatgtt ctccaaatca
      661 ggttgatcat ctctatcaat ttttatatta aattcatttt tttgaatcga taaaataaac
      721 tcgattagct cttccttata agacctatta tattcaatta tgtttatagc catttttatc
      781 tcctttttca tttaatttaa ttataaaatg tgcgtttagt ttgtatctag tgtactcagt
      841 acagcctcaa atgaagtttc attccacttg gcacttaata aagacaagta ttttagcagt
      901 aatacaataa agtccaataa atttccctaa cttcaatatc cactttttaa aaaatgtatt
      961 tttaattaat aaaaaaactc tccccaattt ctatgggaag agctatatat ttaatgtcta
     1021 aacattactt ttatttatta tgaaggaatt agaatcccca agcacctaaa ccttgtgctt
     1081 tgtatgcttt aacagctgcg ttgatttgtt ggtcaacagt gtttgttgga ccccaacctg
     1141 gcatagtttg gaataaacct gaagcacctg atgggttgta agcatttact tgaccatttg
     1201 attcacgagc gatgattgca gcccatgtag aagctgaaac accagtacgt tgagccatga
     1261 tttgagctgc tgatgaacca gtagcacctg cagtattacc attgcttaat ctcactgaac
     1321 ttgaagtagt tgaagtgctg tagttatggt aagttggagc tgaaacagct tcaacgtttg
     1381 agttacttga ttgtgcattg tagcttactg attgtacatt tgaaccttgg ttgtatgaag
     1441 tagtgtagtc tgcacctgca acgtttgaga aaccagcagt ttgaccatta gctgcttcat
     1501 agctccatga ccatgtagta ccatttgaag tgaagttata ttggaaacca tcttttacaa
     1561 agtggatgtc atatgcacca tctttgattg gagctgcatt taattgatct tggtgattgt
     1621 gtgctaagtc aactaagtgt gcttgatcaa cgtttacttc agcagcgtgt gcttgatgtc
     1681 ctgtacctgc tgcgtaacct gttacaccta atgccactgc taatgatgat gccataattg
     1741 tctttttcat agtaaaaaat cctccagtaa taattgtaag tttatgtttt tagtaattat
     1801 attttgaatt tgaatgtcgt agtgcaagtt taaattgtct tttatttctt tcaacggtac
     1861 tcactatatc acaaaaaacc agccagtaaa ttacactttc tttacaaaac attacaatat
     1921 caagttttat ttgtaatgtt gaaatatggc tgttttatac tgtaatgtga aatatgtgcc
     1981 ctttagaatc caataaaccc ttgaaatagt ctttaacgca taagattttt actatattta
     2041 gctcaactat tacagctttc gtaatattac agattgtatt tttgttacat agctgtaata
     2101 tatctgacat gtaacttctc ttattttcgt acattaaacg caattaaaaa gcaatcaaca
     2161 catatgtttc tacacatgta ttgattgcta ttattgttgt atatccaaag ttttaaaaca
     2221 cacatctttt gtgaattgtc ttatctttta taagcacaaa taaactgcac cttaattata
     2281 ttgctaaact ctattatcgt aattcacaat aacattaaat aatttttgat ctcatatttt
     2341 caaaaaacat actgttatta tcccatgaat ttaaaaatat cattagtata taaacgaaac
     2401 actttacgat aaatgatatc tgcaagccaa gctgttacaa atggtacaac aaagaacgct
     2461 actacaatta gtaagacact caaccaagca gaatcaacct ccataaattt aaatgcatta
     2521 atcggtccta ccattcctat aaaaccaaat ccagctgact ctttcgttcc atgaatacct
     2581 actaatgctg ataccaaacc tgatacaatg gctgtcgtta atattggtaa cataagaatt
     2641 ggatatttca ccatattagg tatcatcatt ttaacgcctc caaagaagac ggataacggc
     2701 acccctaaac gattcacttt acttgtacca attatcaata ctgcttcagt cgcggagata
     2761 ccaattgaag ctgatccagc tgctaaacct gaaataccta tcgcaaaggc aatggccaca
     2821 gttgatagtg gcgaaataat aataagacta aataccattg aaatcaaaat actcatgaca
     2881 atcggttgta attctgtaaa accattaacc atattaccga tggctgttgt aatcattttc
     2941 gtatacggca atattaaaac accaattgca cctgaaatac cgccaacaac tgttgggaat
     3001 acaatcaatg ccatactacc tacgcgatgt tgaataagta aaatgaacaa cactgcaatc
     3061 gctgctgtaa tcattgtatt aattaaatca ccaatacccg taatcatcca agcaccattt
     3121 ttaaactgcg ctgcaccgct tcctacatat gctgcactcg ccacaacagc aattgctaat
     3181 ggcgataggt caaatttcat ggcaaccaat gcaccaatca aagcaggtac tgtaaattga
     3241 attgcaacga caacgcctaa taacgtttta aaaatcggat gataatccat aaagtattta
     3301 aaaatttctc caagtatcgc attaggaact aaacccgcaa caatacctat ggcgacacct
     3361 gataaaactc taaatataaa atctttgggt gtaattgttt taattgatgt cataatatca
     3421 tccttccatt tatgtatata catctttatg caaataataa agagccttaa gttataagct
     3481 gccactagct taaattctaa gatgtgcatg ccgatgttgt tatatttagg ctagcagtaa
     3541 catctataac tcaagactat gaaaaatagt atatcacaaa attctgaatt tttagataaa
     3601 gaaattggca atttttcaaa catattgtta caatacactt ttattttatc ttcattttta
     3661 aaatccatta atacaataga agaaagacat tcaaatgctt accaaaaagg tacattattt
     3721 gttaggagcg tatcagcact tatatatcat caacacaatt gacaatataa tagaagatac
     3781 tgataataag tgttaaaaca acagatgtta ggtagtgaac aaatgatgga aagtaaatcc
     3841 atagatccaa gaatcgttag aaccaaacaa ttgcttgtcg atgcttttct taaaatttct
     3901 agagaaaaga aattatctca aattacagtt aaagatatca ctgatattgc tacgctaaat
     3961 cgtgcaacat tttacgctca tttcactgat aaagaagacc tcctagacta cacattatct
     4021 gtaaccattt taaaagactt gaatgataat ttgagcattt ctaatgtcat taatgaaaag
     4081 gttctgcgta atattttcat ttcaattgcg agttatatca aagatgctgc aaagtcttgc
     4141 gaattaaata gtgaagcatt ttgcaacaaa gcacatcaac gtattaataa tgaattagaa
     4201 gatatttttg cgattatgtt agaaaacagc tatccggagc atcaacgaga tatcattgta
     4261 aatagtgcga gttttttagc agctggtatc tcaggcttag cattacattg gtttaacacg
     4321 agtcaagaga cagccgatgt gtttatcgat cgcaaccttc catttttaat tcatcatata
     4381 gcacattttt aataaaactt ggtatttagt catgcatctt gaaatcacta tgtgacttag
     4441 gttcatactt gtacacacaa taaaatttaa cgtattacga ttgattagcc gtgtctagga
     4501 cataaatcaa cgtcctatac tctacaatgt catattagca gtcgttaact gaatgaaaat
     4561 aagcttgtca ttaaaacata tagattttag tgacaagcat ttttgttttt gcgtacttaa
     4621 acaacacttc agacaatatg ttgtttaggc aacaaatgat atgtgcgtgt ttattggcaa
     4681 acgtacaaca tagtagtata gtatgtctaa acaacacatg ttgcatagtt gatatacgtt
     4741 gtttaaatac taagatagga gggattgacg tgagcgagac agatgaacct caggggtttg
     4801 aacgcacgca taatatatta aatattaatc agaataggca gagtctaatg acattcatta
     4861 taagtaaatt gaagtcgacg ttgaagcaac gcataataaa tgctcgtggt aaaaagcgaa
     4921 tcgactatcg actgtcgtat aacttttaca ttacgtatta tgatactgta gaaatcaaga
     4981 aaatcgactg tgaatatacc tatgctatgc ccattgcaat tttaataaga cacacgatgt
     5041 cattcgacaa tgctcatttc tttgctcagt tacgtcatcc tgtcttataa aacaacattg
     5101 cagacatgta tatcaaacga cacttcaata acatcacttt gccatcgtac tactattaaa
     5161 atcgtgtctc aaacccctta ttttaattcc aaaaatctgc tggtcaaaag accgagaaac
     5221 taaaaacatt actggatgtg ttgataaatt atcttataga aataaactca aaatatatca
     5281 acacttgatt ctaaggagga tatgacaata tgaaaatttt agatagaatt aatgaacttg
     5341 caaataaaga aaaagtccaa ccacttactg tagctgaaaa acaagaacaa catgcattgc
     5401 gtcaagacta cttaagcatg atccgaggac aagtattaac aacattttcc acaataaaag
     5461 tggttgatcc aatcggtcag gatgtcacac cagataaagt gtatgatctt cgccaacaat
     5521 acggttatat tcaaaattaa tatttgctca cgaggtattg cacttaaggt gccaactgac
     5581 ctcataaaca aagcccatac tgattgaaga cactaatgtg tccaccatgt ctcttacgac
     5641 atgtgcacat taaaccttca tctttgtatg ggctttttat ttattctttt gagaatttca
     5701 ttttagcaga ccaaaaaatt aaaatggtgt ccatcaagat ctttgaacat tgcaccataa
     5761 aacccttggc ttactgttgg ttcttgaacg actgtgccac ccgcttcttt cactttattc
     5821 accaactggt caacttcttc atttgtgttc acacctagag aaatcatcac ttcatttggt
     5881 tcagtattaa ggctttgctg acttacattt tgaaaatgct tgttttctat taaaattacg
     5941 gttgtttgac ctatttgaat gccgaccatt ttatctaaca tttgtgggtt tctatttatt
     6001 ttaaatccta acgctttata aaactgtgcg ctcttttcta aatcttgcac atgcaaatta
     6061 aaccacattg attgaatcat gattgcaccc cattcattac ttattatagt tttggacttt
     6121 aagccaatca cttaatgata atcttgttgg atttatttca gccattaatt caaagtctac
     6181 ttcataacct ttttcttcca accattgctt ttctgcaaca ccactaacaa attctccttc
     6241 tataacagta gatttacctg tcacttcact aaaaattgtt gctgcttcac ttaatgtaac
     6301 ttcatcggaa ccaatctcta ttgattgatg cgtaaagctt tgtggatgtg caaaaatata
     6361 cgatgcaatt ttagctatat caatagaaga aatcattgtg aattttatat tcggattaat
     6421 aaattctggt aatgtaatac gttcatcttc gactttagca atgcgtaaaa aattatccat
     6481 aaagaatgat ggtttgataa ctgttgcatt tatattagat tccattaatc tattttctat
     6541 ttttgctagt acttcaaagt gtgggccagt tcgatttcga ttaacccctc ccgcagtact
     6601 atacacaata tgttgaatat tttcttgctc agctatttca attatcttca taccttgtct
     6661 taattcttcg ctaacatcat ctttaacgat tggctgaata ctgtataagc catacttacc
     6721 tttcatcgct gattgcaaac taacattatc actcagatca ccttcaacga ttgataaatg
     6781 cggatgtcct atgtctgaaa gtttacgatt attcttattt ctagttaatg cacttacata
     6841 ccatccatcc tctaacaact gttttacaac tgcattacct tgcttccctg ttgcgcctat
     6901 tactaaaata tctttcattt tagatccact cctcttttta tcttaaaaac caatagtcct
     6961 ctttttcacc ttttaaatac gtatatggtt ctggtgtttg tccatttcct ttaatattgt
     7021 cgtttattac atctctgaca tcccatacta aatgaataaa tcgttgtaat tgatgactaa
     7081 aaatgcctag tggcgctgtc atagctaata ctttttccac atcttctgta gtgacattca
     7141 gtttttcagc aatcatttga accgataagg tagattcttc aaaatgtgct tgcaactctt
     7201 cgcacgtaat gcgtctttgc tctttattta aagacatatg caacactctt ttctttttta
     7261 aatatttaca gcactttgca aattcattgc tatctttctt aacacgtttt ataataaacg
     7321 gagagttatc cgtttgtcaa ttttagagaa ttttaagtag gtgtaatcat gcgaaaagat
     7381 gcaaaagaga ataggcaacg aattgaagaa atagcgcaca aactttttga tgaagaaggc
     7441 gttgaaaaca ttagtatgaa tcgtattgct aaagaactag gtatcggtat ggggacattg
     7501 tatcgacatt tcaaagataa aagcgattta tgctactacg tcatacaaag agaccttgat
     7561 atttttatca ctcattttaa gcaaattaaa gatgactatc attctaatta tgaggttatg
     7621 caagtgtcgt tagattatct tttacaattt aaaatcgata ataaggcact actgcaatgt
     7681 attgaagcag gcaacaataa attacgtttt taccaaagcg cattttatca agaattattt
     7741 gatttttatt atgacttatt taaaagcgat gacgacactt acacgaagtt caaaacagat
     7801 atgctgttgc aatcattatc aactagtgta tttgccttcc aaatagagca tcgccatatt
     7861 tcaattgaag cgtacagaaa ttatttatta aacatttatt tagatgaggt ggaacgtaat
     7921 gactaaaatt gtgttaatca caggaggcaa taaagggtta ggctatgcaa gtgcagaggc
     7981 acttaaagca ttgggttaca aagtttatat aggttctcgg aatgatgtaa gaggacaaca
     8041 agcatcacaa aaattaggtg ttcattatgt acagttagat gttacaagtg actactcagt
     8101 caaaaatgct tataacatga ttgctgaaaa agaaggccgt cttgatatcc tcattaacaa
     8161 tgcaggtata tctggtcaat tttcagcacc ttccaaattg acacctcgtg acgttgaaga
     8221 agtatatcaa acgaatgtat tcggtatcgt acgaatgatg aatacattcg tccctctctt
     8281 agaaaaatct gaacaacctg ttgtcgtcaa cgtatcaagt ggtttaggtt catttggaat
     8341 ggttacaaac cccgaaacag ctgaatctaa agtgaattca ttagcttatt gttcgtctaa
     8401 atcggcagta acaatgctaa cgctacaata cgctaaagga ttacctaaca tgcaaatcaa
     8461 tgctgctgat ccaggcgcta caaatactga tttagttggt gattttagta ataattccaa
     8521 acacgtttct gaaggaatca agccaatcat tcaattagca acgattggtg cagatggccc
     8581 aacaggtaca tttattaatg gcgatggaga gatgccttgg tagatcaatt ttatgttaca
     8641 aacattgaaa ttagaggtat ataaaaaatg cttctgcaat agatgcaata aacatgcaat
     8701 acaatatgga ggcgaagtaa atgaaaagta ttacgtttga agaacattat gtcattgaag
     8761 atattcaaaa agaaacgatg aatgcgatat cagcagatcc taaaggtgta ccgatgaaag
     8821 taatgttaga aggccttgaa aaaaagacag gttttacaaa tgccgacgaa ttatcacatc
     8881 atgatgaacg tatccaattt atgaataatc aagacgttca aattcaagtc ttatcttatg
     8941 gaaatggttc tccttcaaat ttggttggtc agaaagccat tgaattatgt caaaaagcaa
     9001 atgatcaatt ggcaaactat attgcacaat atcccaatcg ctttgtaggc tttgcaactt
     9061 tacctatcaa tgagcctgaa gccgcagcgc gagaattcga acgttgcatc aatgatttag
     9121 gattcaaagg tgcgctcatt atgggacgtg cacaagatgg ttttcttgat caagacaaat
     9181 atgacattat tttcaaaaca gctgaaaatt tagacgtacc gatttatcta catcccgcgc
     9241 cagttaacag tgacatttat caatcatact ataaaggaaa ttatcctgaa gtaactgcgg
     9301 caacatttgc ttgttttggt tatggttggc acattgatgt cggtattcat gcaatacatc
     9361 tagtattatc tggtattttt gatcgttatc caaagttaaa tatgattatt ggacattggg
     9421 gtgagtttat cccattcttc ttagaacgta tggatgaagc tttattcgct gaacatttga
     9481 accaccctgt aagctattac tttaaaaata atttttatat cacaccgagt ggcatgttaa
     9541 cgaagccaca gtttgattta gtcaagaaag aagtaggtat tgatagaatt ctttatgctg
     9601 ctgattatcc atatattgag cctgaaaaat taggtgtatt tttagatgaa ctgggtttaa
     9661 cagatgaaga aaaagagaaa ataagttata caaatggtgc taaattatta ggtttatcag
     9721 ctaacaatta acaaatggag gtcattgaat atggaaactt tagaattaca aggcgctaaa
     9781 ttacgatacc accaagtcgg acaaggaccc gtgctcatct ttattcctgg tgcaaacggt
     9841 accggagaca tttttctgcc tcttgcagaa cagttaaaag accattttac tgttgtagcc
     9901 gttgatcgtc gtgattatgg agaaagcgag ttaactgaac cactccctga ttccgcttca
     9961 aaccctgaca gtgattatcg tgtcaaacgc gacgcacagg acattgccga acttgcaaag
    10021 tcattaagtg atgaacctgt ctatatatta ggttcaatcg ttgcgatgca tgtgttaaaa
    10081 gattaccctg aagtcgttaa aaagattgca tttcatgaac caccaattaa tacattttta
    10141 ccagacagta cttattggaa agataaaaat gatgacattg tgcatcaaat tttaacagaa
    10201 ggcttagaaa aaggcatgaa aacatttggt gaaacattaa atatcgcacc aattgatgca
    10261 aaaatgatgt ctcaacctgc tgatactgaa gaaggacgta tagaacagta caaacgtacg
    10321 atgttctggt cagaatttga aattagacaa tatacgcatt cagatattac tttggatgat
    10381 ttcacaaaat acagcgataa aatcacccta ctaaatggga cagattctag aggctccttc
    10441 ccacaagatg ttaactttta tataaacaaa gaaactagca tacctattgt agatattcca
    10501 ggcggtcact taggttatat tcaaaaacct gaaggctttg ctgacgtatt attaaatatg
    10561 tggggttaat ctatcgttct ctaataaaac agcttggaca gttttcaaat tatatttgat
    10621 ggctgaccaa gcttattttt gtttacgttt aaaagttcaa atcaactcgt tattatacat
    10681 gaaggcatgt taaccattct cttccactga aaacactact gttccaaaca ttgatagtct
    10741 tcttttgaaa taccctttcc gataagcacc aaatattctg gcactttttt tgaaaatgct
    10801 acaggtgtta attcatattg tccttgtgta aattgaatga gatacgtatg tgcggtgtct
    10861 tcaaatgtca taaacccttt caacctataa atatgagacg gaaggcatgc taaacgtttt
    10921 ataaactctg ctttagtaca tttggctggt gattgcacaa attgatgatt aatcatttga
    10981 tgtaacgact tatgtttatt gtcagaagtt gctgtcattt gtctaactga tattggcaaa
    11041 gtgacgctgc catgcatacc gacttgtata tcggcctctg ggttaataac ttctaaatct
    11101 ttcaatagtt tgctcgttgt ttcaacatct gctgaatcta ttttattaac aaataagaca
    11161 gaacaatatg ctaattgctc ataaaataag ccttggatgt ctttagggaa tgatttaatg
    11221 tgtttataca tgcttgcgtc tattacacca atcatatgtg taattgttgt aaacggagct
    11281 aaaataggcg ttaaacaagc atctaagaca gagactggtt ctgcaatccc actacattca
    11341 ataaatacaa tgtctggttg ctcttttaaa tataattgat gtaactgttc tgatacatct
    11401 gctttcattg cgcaacagat acacccctct gtcagttcac ttaaaggtat gtcttcagat
    11461 actaaggcac catcaacact cattttccca aattcattca tgatgactgc tggtttcagc
    11521 gattccttta atgattcagc gagcaaatga tttaagacag tcgttttacc gccacctaaa
    11581 aacccaccta taataactat tttcatctgc gactctcctc ttatacttca tatgaatagc
    11641 atattccaag atagatctcc tattgactga catgactcta tattcagtta tagttcattt
    11701 ttttaaataa tcagcattac tattttaaca atgatgccta caccaataac taaagacaat
    11761 gatgtcacca tttgctttcc aattaatttt agtgctgact gaccacctaa atgtttcaaa
    11821 agcatcgtca ttgtgaccga gcacgctgta aaagttgaac taaaaaacac aagtagtagt
    11881 aactgtgttg ctgtcattcc ttgtaataag gcgccctgat gcaaattaaa caagagcatg
    11941 ccgtcttttc gaatcattga aaataaaatc cctggtgaca attctgacga gatgcctaat
    12001 aacgataata taggtgtaaa tatttgtgat aaaacattca aaattggcgt tagtgataaa
    12061 atactaacaa taagacagat tgtgataaaa ataggtagcg cttgaacgat aaacatttgt
    12121 atattttgcc acatttgcag caacatttga cgtatatttg gcatatgtaa ttgtctatca
    12181 taaggtagcg gaacgctaag ttgttgatca ttctttttca accatatcct attatgtaag
    12241 atgccaccta aaagtactaa tattaagtac ggcataaata gccatgactt tccagctaca
    12301 ctaaaaatag ataatgtcgc acctatttga taactacaag aactaccaaa gcttattaaa
    12361 ctcatacact gtgtcttcgt gcaggtatgg cattggtgtg ctgcttgtga aatagctgct
    12421 gcattacatc caaatccttc taatagtggc acgatatcat taccttgtaa tccgatccat
    12481 aacattgacg gttcaattgc ccatatcatc catgatttga gtcctgtttg atcaattatt
    12541 gcagttgata aactaattaa tattacaacc ggcaatgccc atacgagcga atatgttcca
    12601 agtgatagca caccataatc accaaataat atatgctgta atatatcgtg attgagcaat
    12661 gttgtttgag ttaaatattc tatccatgct gatacatact gattttgaaa ccaatctgaa
    12721 aattgataag cgataccgat tggtaatgca aacattaaaa atatcaacaa ccaggctaaa
    12781 aatgtcgcac ttattttagg ccttaaatgt cgtcgctcat gttgaaatag tgtcaattgt
    12841 tctatcacat gttcgctcgc tataggcgaa gaaactacac tttctgccaa tgaaatatct
    12901 aacttctgtt ttatgattaa taatcttaat ttcgcttttt ctgttgcggt aagtttatcc
    12961 gcatgactaa taactaaata atgaggttca ttaggtatgg gcgtataatg cccagttctc
    13021 atatcatatt tcacttctaa catgtgcatg acacttcaca acatgaataa tcatctaaat
    13081 acatttgatt tttttgataa ttttcaatga catcttgttt agctggtaag ccttcccgct
    13141 gtgttaaaag atgggcaagt actgcaaatc gcgctctgaa tttatagata tagcataatt
    13201 tggcattatc attttcaact gttgcaccaa tcataaaaat attcggataa cgtgtcgatt
    13261 catcatgtgt tgttaattta atatcttgat ttgttgtcac aaataattgt tgaacgattg
    13321 gattttttgt tgcatcaaag ccagttgcta gtattggttc atgaggcgta tgcacacttt
    13381 gtccgctatc aaaactgata tgatactgtc cattgttaaa atcaatatct ttaactgtat
    13441 aatgtacatt catttcgatg cgagcacctt gcttaatgac attacctagt cgctgacgtg
    13501 tataaggtga caatctaaca ctaggatcag catccggatc atttaaaccg gttgtgctag
    13561 tataaagtgc gatgtcagag ccattttttg caagttgata tgcagcatca aagccacttt
    13621 cattacctcc gataaccaca tattgcccct tattaaagtt atcaaagtct tcaatttcac
    13681 tataatgaat accatattta aatggctttt tagggaaatt ataatcacct gttgcgacaa
    13741 agatataatc cgcgtgatat atctctgttg tcgttgcaat cgtataatat gcatcatcta
    13801 cagatatatt tgtgacaact gtattttcaa agatattcag ctcgtaatgg ttggcaacca
    13861 cttgtaaata ttcagcatat gtttctccgg aaatatgttc ttcattaaat gtaaatgctg
    13921 gtgaagtatc catggaaatt gcattcatat caggcatgcc aaatccatta gacgtaaatg
    13981 atggcgtgat cgtacgagtc gatttcggcc aatgtttaaa tgaatgtcct actgttcctt
    14041 tttctaaaat aatgacatct gttataccga aatcttttaa ggtaatggcc atacctatac
    14101 ctgcagcacc ggcaccgata atagccactt tatgatgttg catattaaca ctccctcaat
    14161 gatctatttc aagttattgt ttttgactat aaatcgtaat aattacgatt tataaagtat
    14221 agacctatcc ttgagaaatt tcaatattaa aagaaaaact tccgttattg tagagaattc
    14281 atctacattt cggaagttgg tatgtgtctt ttttattaaa tttaaagatg ctacggttag
    14341 ttcaaatgat tatgaatgac atttcatggt atcatccagc ctcagtactt ggagataaca
    14401 ttttggcggt gagacagaaa tcatattttt acaaaattta ttggggtgta acttctccac
    14461 gttgtatttc actatccaaa ttttgtttca tattttcaaa caactcttga aatctaacct
    14521 catcttgatc agaataacct ttaaaaataa ctgaatctat attttcctcg ttaaaataat
    14581 aaatttcatc ttctacaaca ccaatcggat atttacatgc agaatagtca aacatatact
    14641 tttgattttc aatttctaca attggtcctc tattaataat cattaacttt tgcgaacctt
    14701 cttttaaata aataatgctt cctattgttt ccatttattt tcctcctttg tcaccgtctc
    14761 aatttcaaca ttccttcgca tttccaagtc aatcatctct tcttctgctt tacgaatgcc
    14821 atgttgaatt tgctgttgct gctcgtataa cgcttccgtt gaacgtcgtt tcgcaaaatg
    14881 aaattcatcg cggaactgtt ccatacgact agccatattg ctactataat aacgctcacc
    14941 taaatgttga tataattgat ctatcgaatg attaagcgca tcactttcat ctatcaatgc
    15001 tttaacctct cgcttttcat catataagtc atccatttgc ttatgtttcg ctgtgattat
    15061 tttattgatt tcatccagtt tattcgacat catttcacca cgttttaatt tttaccaatt
    15121 tcattaccaa tcattacgtc agtttggtca aaagcctcac caactttttg tatattatcg
    15181 ctcgcagttc gtattgcttc agcaacagat tgattcttac tcatcaaatc atctatcaca
    15241 ctatgagcat tattatttcc acttactgtt gtactttcat ctttatccgg tttcgacaca
    15301 ttattacatg tttcaacagc gcttttaata gatgatgatg cactattggt cttaaaaata
    15361 tcactttgaa ctgtactcat catttacacc tctctctcca ttttaatta
//
LOCUS       DBNENW010000054        15363 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNENW010000054 DBNENW010000000
VERSION     DBNENW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.054"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(155..256)
                     /locus_tag="AC1LW8_001355"
     CDS             complement(155..256)
                     /locus_tag="AC1LW8_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773198.1"
                     /translation="MCQYLCLRTIELGVFIVMIYCLKVVALTVRLHN"
     gene            314..754
                     /gene="hypR"
                     /locus_tag="AC1LW8_001356"
     CDS             314..754
                     /gene="hypR"
                     /locus_tag="AC1LW8_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional regulator HypR"
                     /protein_id="HIP4773199.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLSNDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYEQDEQNIIINFYKGKTIKDVIEDIQKEDLCHENI"
     gene            741..2065
                     /gene="merA"
                     /locus_tag="AC1LW8_001357"
                     /pseudo
     CDS             741..2065
                     /gene="merA"
                     /locus_tag="AC1LW8_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothiocyanous acid reductase MerA"
     gene            complement(2117..2458)
                     /locus_tag="AC1LW8_001358"
     CDS             complement(2117..2458)
                     /locus_tag="AC1LW8_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HIP4773200.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(2635..3711)
                     /locus_tag="AC1LW8_001359"
     CDS             complement(2635..3711)
                     /locus_tag="AC1LW8_001359"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HIP4773201.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHHCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            complement(3724..4707)
                     /gene="mvaD"
                     /locus_tag="AC1LW8_001360"
     CDS             complement(3724..4707)
                     /gene="mvaD"
                     /locus_tag="AC1LW8_001360"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIP4773202.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            complement(4712..5632)
                     /gene="mvk"
                     /locus_tag="AC1LW8_001361"
     CDS             complement(4712..5632)
                     /gene="mvk"
                     /locus_tag="AC1LW8_001361"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIP4773203.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            complement(6219..7055)
                     /locus_tag="AC1LW8_001362"
     CDS             complement(6219..7055)
                     /locus_tag="AC1LW8_001362"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIP4773204.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(7058..8044)
                     /gene="pta"
                     /locus_tag="AC1LW8_001363"
     CDS             complement(7058..8044)
                     /gene="pta"
                     /locus_tag="AC1LW8_001363"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIP4773205.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDV
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            8212..8964
                     /gene="hemQ"
                     /locus_tag="AC1LW8_001364"
     CDS             8212..8964
                     /gene="hemQ"
                     /locus_tag="AC1LW8_001364"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HIP4773206.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
     gene            9513..10277
                     /locus_tag="AC1LW8_001365"
     CDS             9513..10277
                     /locus_tag="AC1LW8_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP4773207.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            10290..10742
                     /locus_tag="AC1LW8_001366"
     CDS             10290..10742
                     /locus_tag="AC1LW8_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP4773208.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(10883..12367)
                     /locus_tag="AC1LW8_001367"
     CDS             complement(10883..12367)
                     /locus_tag="AC1LW8_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIP4773209.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGTLLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(12488..12856)
                     /locus_tag="AC1LW8_001368"
     CDS             complement(12488..12856)
                     /locus_tag="AC1LW8_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIP4773210.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(12989..13369)
                     /locus_tag="AC1LW8_001369"
     CDS             complement(12989..13369)
                     /locus_tag="AC1LW8_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HIP4773211.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(13370..14026)
                     /locus_tag="AC1LW8_001370"
     CDS             complement(13370..14026)
                     /locus_tag="AC1LW8_001370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIP4773212.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            14210..15040
                     /gene="thiD"
                     /locus_tag="AC1LW8_001371"
     CDS             14210..15040
                     /gene="thiD"
                     /locus_tag="AC1LW8_001371"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIP4773213.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         4927 a         2858 c         2344 g         5234 t
ORIGIN      
        1 agttgttgaa aatatttcct catcatgcga aatatcaaaa cgtatctaaa atacgaataa
       61 gtttatacaa tcacacaaca tcatcattca aaattttatt gcccttattt tcaatattca
      121 tttcatatct atcagttcat acataactaa aatttcaatt gtgcaatctc accgttaatg
      181 caacaacttt caaacaataa atcatcacaa taaacacacc taattcaata gttctcaaac
      241 ataagtattg acacattgag caaaatgatt tttaattgta actaatatag ttacaattat
      301 gaggtgagaa acattgaatt tagaatttaa cattgccgtg catgtattag cttttttaac
      361 taagcatcat tcagaaaaat tcaatagtag ttcattagca gaattaactt gtttaaatcc
      421 tgttcaatta cgacgcgtga cgactcaact tgtcgattta aaaatgatta acacaatacg
      481 gggtaaagat ggtggttatt tatcaaatga tcgtagcgct gatgtctctc tagcaacatt
      541 atataaacat tttgtcttag agaaagaaca tcacacacga ctatttactg gcgacgaagg
      601 cagtcactgt caaattgctc gtaatattgc aactactatg tcacattatg agcaagacga
      661 acagaatatc attattaatt tttataaagg aaaaacaatc aaagatgtca ttgaagacat
      721 tcaaaaggag gatttatgtc atgaaaacat atgatttaat tgtaatagga tttggggaag
      781 ctggtaaaac tttagccaaa tatgctgcat caacaggtca acacgtcgca gttatcgaac
      841 aatctccgaa aatgtatgga ggcacttgta taaacatagg atgtattgct tcaaagacac
      901 ttgtacatga tggattagaa ggcaaatctt ttgaagcaag ttataaccgt aaaaacgatg
      961 ttgtcaatgc gctaaacaat aaaaattacc atttattagc agacgataac aacattgatg
     1021 tactggattt taaagcgcag tttaaatcta atactgaagt gaatttatta gatcagcatg
     1081 acgatatcgc cgatagtatt actgcacctc atatcattat taatactggt gctacctctg
     1141 ttattcctaa cattaaaggc cttgatcaag ctaaacacgt cttcgattca acaggtttat
     1201 taaacattaa ctatcaacct aagcacctcg ttattgtagg tggcggttat atcgccttag
     1261 aatttgcttc aatgtttgcg aatttaggta gtaaggttac agtattagaa cgtggtgaat
     1321 catttatgcc acgtgaagat caagatgtcg ttgcacatgc tattactgac ttagaaaata
     1381 aaggcattac attgcataca aatgttgaaa cgactgaatt gtcatctgac aatcatcata
     1441 caacagtcca taccaacgtt ggtaactttg aggctgatgc agtacttttg gctatcgggc
     1501 gcaaaccgaa tacggattta gctttagaaa atactgatat cgaattaggc gacagaggcg
     1561 aaattaaagt caatgctcat cttcaaacaa ctgtaccgca tatttatgct gcaggtgatg
     1621 ttaaaggcgg acttcaattt acgtatatat atctttagac gattatcgaa ttatcaaatc
     1681 agcgttatat ggtaatcagt cacgtacgac tgacaataga ggtagcgtgc cttatacagt
     1741 atttatagat ccaccattat cacgtgttgg attaactagt aaagaagctg ccgctcaaca
     1801 ttatgattac actgaacatc aacttttagt aagtgctata cctcgtcaca aaattaacaa
     1861 tgatccaaga ggtttattta aagtagtcat taataatgaa aataatatga ttttaggtgc
     1921 tacattatat ggtaagcaat ctgaagaatt aattaatata attaaacttg cgattgatca
     1981 aaacattcca tataccgtat tacgagataa tatttatacg catcctacga tggccgaatc
     2041 atttaatgat ttatttaatt tctagacaaa acataaaaac ctggtggcac gcattgaatg
     2101 atgctgccat caggctttat tgttgtgctt ttcgcttttc taatttttct ttaagctttc
     2161 tatcttgttc ttctttacga cgtttacgtt cttcatgtcg ttttcttaaa cgctcttctt
     2221 cttcaggatc acgtggtttc tttaattgtt gagaaacttt ttcgattaat tcttcttcag
     2281 taagcgcagc cagtgggcgg ttattaacaa aagtgaatgt ttttcggcgt ccaggtccac
     2341 aataagattg acaacctatc acgatttcag catcgggatc taatttttcc aacttctttt
     2401 gtaacgttct tatattgact gcctgacatt catcacaaat aaggaatgta tttttcatat
     2461 tgctacccac ctttctttat catatctata tcgtcgattt cattaatttt ttcgttaact
     2521 ctatctattt tactcttttt aatatttttt tcaagatacg taacacggct ggcaataaaa
     2581 aatggagcat ttatcttcta attaaattag atgattgctc ccctatcaaa tcatttattg
     2641 cccatgataa atattaaatt ttaatggttt aataccatgt tttgtccatt catcataaat
     2701 tttttcttta tctacatctt tattgataat tgtaatacca cagtctccac caccagcgcc
     2761 tgatgtttta gatgcgccgt gatacttttc agcaatatca cacaaatatt ttagcttttc
     2821 agtttctata tcaactgtag cttctttatc catacgttga ataattgtac gattctgacg
     2881 caccatcttt tgcacacctt taatgttatt tgttttaaaa gcatgaataa gtttttcaac
     2941 acaatgatgt gaatcttcta agaagtcacc gtaaaatgaa ggatctgatt tcaaacgttt
     3001 cacttcgcta acaaagtgtg gtgatgacgc cggtgagcca gtccaaccga taagtacttc
     3061 catattttca ggtgcttgca atggttcgat gtgcaatcca ggccagtttt tgattaaaac
     3121 ttcttcaacc gtagtatctt caatttgatg cttaacccat tcatgatcaa aagtactata
     3181 cgctaaccat ccactatata cactcacagc aatatctccg catgaactta aactttgtaa
     3241 cttcatattt gcaatcactg ctagtttata aatgtataaa ttagataact tcatatcata
     3301 aaattcattt aatactttta taactgacac aagtactgct gcacttgaac ctaatccata
     3361 tttatgacca tttgaatcat ctaaattact atcaatagtc agatgaaaat gcttcatcgc
     3421 tatatcgcaa cttttcgcgt attgttcaaa tatttcaata gctgtgacca cataatttaa
     3481 ttgttttgct gcatgtggat ctgaaatgac aatactatct tcatctctac taaatgtaac
     3541 tgggttatga tgtaatgctt ttgaatgaat ggtaccttta tattggtctg cttcttcaat
     3601 agtagcagtt acaaaacgat ctaacgcaat aagtacagat ttatatcctg gttctgttac
     3661 agcatattct ccagcaatat aaagttttcc gggtgctttg acctgaatca ttttatctct
     3721 tccttactca attatttcaa ttcctgtggc aataatgtca ctatcaataa tttggttatt
     3781 atcaaactgt gttaataatt tatctataat ctgttgcttg tttttctttt ctacaagtat
     3841 tttcacatta ggtcccgcat ccattgtaaa ataacacgga taccccgctt ctcggcattc
     3901 gtgaacaagc gccatgacat cataactttc ttgcacaaga tatgtgaacg gcggtgttga
     3961 tcctagattc gtggcatgca tacgcaaacc attttcttca attacttcac caaggcgttt
     4021 aaaatctttg tcttgaatcg ctgcttttgc ttcagctaaa tcttcatcaa tatgatctaa
     4081 ccaatattga taaaaccttg atgtgtttcg tgtcaatgac ataccatatc gactaggtac
     4141 ctttttagaa tgttgattaa tcacaacaaa tatcatggca aggtcatctt caaaatgatt
     4201 cgattcaagt ggaacggcat atgacgtctc atcactatac cctttttccc attctgcaaa
     4261 tccaccataa atactacgcg acgcagaacc cgaaccaatt cgcgccaatc tcgataaatc
     4321 cttatctgac agctgcatgt ctagcgcttg attacaagct gctgctaaag ctgcatatgc
     4381 gcttgccgat gaagccaacc ctgctgctgt tggtacaaaa ttgtcgcttt caatttctgc
     4441 ataccaatcg atgccagctc tatttctgac aatatccata tattttgaaa ttttctctaa
     4501 ttctttgcca ctaacctttt caccattcaa ccaaaattga tcctgtgtta actggtcgtt
     4561 aaaagtgact ttcgtttcag tgtaaaattt ttctaatgta acagatatgc tattattcat
     4621 tggaatgatt agtgcttcat cttttttacc ccaatatttt ataagtgcaa tattcgtatg
     4681 tgcacgtgct ttgccacttt taatcaacgc attaacctcc taaattctca atccaagtat
     4741 gtgctgcacc agctttttct acagctttta caatattttt cgctgttggt aaatctttgg
     4801 caagcaataa catacttcca ccacgaccag cgccagtaag ttttccagca atcgcaccat
     4861 tttctttacc aattttcatt aattgttcta ttttatcatg actaactgtc aacgccttta
     4921 aatccgcatg acattcatta aaaatatccg ctaaggcttc aaagttatga tgttcaatca
     4981 catcactcgc acgtaaaact aacttaccga tatgttttac atgtgacatg tactgagggt
     5041 cctcacaaag tttatgaaca tcttctactg cttgtcttgt tgaacctttc acaccagtat
     5101 ctataacaac catatagccg tctaaactta acgttttcaa cgtttcagca tgaccttttt
     5161 ggaaccaaac tggtttgcct gatacaatcg tttgcgtatc aataccactt ggtttaccat
     5221 gtgcaatttg ctctgcccaa ttagcctttt caatgagttc ttctttcgtt aatgatttcc
     5281 ctaaaaaatc ataacttgca cgaacaaaag caaccgcgac agctgcactc gatcctaatc
     5341 cacgtgatgg tggtaaattc gtttggatcg ttactgctag cggctctgta atattattta
     5401 attctacaaa acggttcacc aaagacttaa gatggtcagg cgcatcatat aacataccat
     5461 cgtaaacatc gcttttaata gacgaatagt tcccgctctc taaggcttct attaaaactt
     5521 tgattttacc tgcgttaaac ggtactgcaa tagcaggctc tccaaatgta acagcatgtt
     5581 ctcctattaa aataatctta cctgtcgatt ccccatatcc ttttcttgtc atgtcaatat
     5641 caccttttat atttatccta tacttgattc attattttta tttattagta aaagacatca
     5701 tattctaagt tgcatacgca ttcgcgttaa atttcattgc agtctttatc tcacattatt
     5761 catattatgt ataatcttta ttttgaattt atatttgact taacttgatt agtataaaac
     5821 taactttcgt ttacttcaaa gtttaaatct tatcgagtga tatttcagat tctttatctt
     5881 tttataaaat agccctacaa tttataattt tccaccctaa ctataatact acaaataata
     5941 attggaatat atagatttac tactaaagta ttagaacatt tcaatagaag gtcgtttctt
     6001 tcatagtcat acgcattata tataccctat tctcaatcta tttaatacgt aaaacatgaa
     6061 attttcttat taaatttatt atttccatca tatcattact tttaatttaa tgatgttcaa
     6121 tttaaatatt aggtcaataa catatttatg ctttttatgg atactttcaa aaataacagc
     6181 cccaaacgat aacttgaaag gggctgttaa atatttaact attgcatttg atctatcatt
     6241 ttcttgtttc tttcaatcat tttatcaaaa tacgtatcgt atctttgcca ttcttcttga
     6301 gtaattggcg tcatatttaa tacaccgcca agatctttga ttgcatataa tagtaaaaac
     6361 attacatctt gaacagtaat cgttttgtta agcaatgtct ctaatgaggc catacaagat
     6421 ggttcaattt caggatattt aaatttagtc acttcacctt ttaaagcatg ttcataaaat
     6481 gtttgcatca tcaatgcacg ttctgaacca gagccttcaa cacaaagata aatttgtaca
     6541 gcaataccgc ctctaactct tcgttgcgat atgcctgcaa atttcttacc atcgatactt
     6601 aagtcaaatt ttcctgggca ataagaatgt tcaatttcca tcgtatcaat atcaacattc
     6661 tcattttcga acattttgct aattaagagg tacatcacag taaacgcttc atcaatcgtt
     6721 gtttctgttt gtcctttgaa catcagcgat atatttaata caccttgatc tagaacgaca
     6781 cctaagccac cagaatttct aacaatggca ttataaccaa tctcattcgt taaataatca
     6841 atgccatctt ttaaaaacgg caatcttgaa tcatgaatac caagaataac agtatgttga
     6901 tgaatccaag tacgcacaac attatctgat atatctttgc ccacactttc gcaaaatgta
     6961 tcatcgaatg cgaaagattg cataggttct aatccagaag aatgatcgat atatcgccag
     7021 ttgacgccat taaaatattt actcgctaaa tccatcgtta ttgtaaggct tgcgctgctg
     7081 tgataattga aagattgtat acatcttcaa ttgagcagcc acgtgacaag tcatttactg
     7141 gagaatttaa accttgtaat actggaccaa ctgcatcata tccacctaaa cgttgtgcaa
     7201 ttttgtaacc aatattacca gcttctaaac ttgggaatac aaagacattt gcatcacctt
     7261 gtaatttagc acctggcgct tttttctcag caacacctgg tacaatcgca gcatcaaatt
     7321 ggaattcgcc atcaatgatt gcttctaatt tttcttcttc agctttttgt tgtgctaatt
     7381 tgacagcttc ttgaactttt gtcacgtcgt ctgatttagc agaccctttt gttgaaaagc
     7441 ttaacattgc aacttttgga tccatgccaa agcttaatgc tgattttgca ctttctactg
     7501 caatttctgc aagtccttgt gaatcaagtt ctggattgat tgcacaatca ccaaagatgt
     7561 attgtacatc acctttaatc ataaagaaga tacctgatgt tcttgataca cctggtttcg
     7621 ttttgatgat ttgtaaagct ggacgcacag tgtctcctgt tgaatgtgct gcaccactaa
     7681 ctaaaccatc tgctttacca gcataaacaa gcattgtacc gaagtagttc acattgttta
     7741 ataattcttg tgcttgttct tcagtcgctt tacctttacg tcgttcaaca aatgattgaa
     7801 ctaattcagc tttcaattca cttgtcgcag gattaattaa ttcaatatta gaaatatcaa
     7861 gatcaagttt ttgcgctaaa gattgaacct tagtctcatc acctaacacg attggtgtaa
     7921 cataatctgt tgcttgtaat tgtgttgcag ctgttagaac acgttcgtcc tctccttcag
     7981 gtaatacgat tttaacgttt ttaccagaaa gtttgtcttt taatacattt aataaatcag
     8041 ccataatgtc ctcctgtaat ataaatctta ttaatcattc acggtataat tatacgccat
     8101 tttatttcat aatgccacgt caccaatttg taatcataat tttatcgtta cccctttgct
     8161 ttatgataaa atttataaag aactgatgat ttttgaaaag gagcgataaa catgagtcaa
     8221 gcagccgaaa cattagatgg ttggtatagt ctacatttat tttatgcagt tgattgggca
     8281 tcattacgtc tagttccaaa ggacgaacgc gatgcacttg tcactgaatt tcaatcattt
     8341 ttagaaaata cagcaactgt aagatcatca aaatctggtg atcaagctat ttataatata
     8401 actggtcaaa aagcagattt gttattatgg ttcttacgtc ctgaaatgaa gtctttaaat
     8461 catattgaaa atgaatttaa caaattgcgc attgctgact tcttaatccc tacatattca
     8521 tatgtatcag tcattgaatt gagcaattat ttagctggta aatctgatga agatccttat
     8581 gagaaccctc atatcaaagc aagattatac ccagaattac cacattctga ttatatttgt
     8641 ttctatccaa tgaacaaacg tcgtaatgaa acttataact ggtacatgtt aactatggaa
     8701 gaacgccaaa aattaatgta tgaccatggt atgattggta gaaaatatgc tggcaaaatc
     8761 aaacaattta ttactggttc tgtagggttt gatgatttcg aatggggcgt aacattgttc
     8821 tcagatgacg tattacaatt caaaaaaatt gtatacgaaa tgcgctttga tgaaacaaca
     8881 gcacgatacg gtgaattcgg tagtttcttt gtaggacatc ttattaacac aaacgaattc
     8941 gatcaattct ttgcgatttc ttaatacatt ggtacgttta taaattaata aaaaaattcc
     9001 aagcttatcg gtttaagctt ggaatttttc gtttatcttc agtatattcc cgtatacata
     9061 agacgtgatt tggtaaatag ttgaaatctg tatgtttaaa cttatatata tgtgctaatg
     9121 tattatcaat aacaaagtac actttgctca tagcaaagtg tacccgagta gtcttccttg
     9181 ggagaacttt aactactatc actacatata aacgttaacc tcaatagaaa ttatacagtc
     9241 gctactctat acaatttttg taatggttaa ctaatattat tttaacctat ttgaaatatt
     9301 tgaaacatat ttttgtcgaa ttcttttcaa taatttttcc tttttatact tcaagagaat
     9361 tttaactact aaaaattccg atgattatta ttacaatagt atcaaatatt agttttttaa
     9421 aatcaataac aacttatcaa aaagctcatg tggttatttt atagtgtata aactataatg
     9481 agtattaaat tcttataaac aatggtgatg aaatggacat aaattcagaa gaatacaaac
     9541 aagaggtact tatcaaagac gttgtcatgc ttgctgctcg catactatta gaatctggtg
     9601 cagaaggtac gcgtgtagaa gataccatga cacgtattgc aaaaaaactt ggttacagtg
     9661 aaagtaacag ctttgttaca aacactgtca tccagtttac gttacattcg gaatcgtttc
     9721 ctagaatatt tagaattacc tctcgagata caaacttaat aaaaatttcg caagctaata
     9781 aaatttcgcg tcaaattaca aacaatgaaa tttctttagc cgaagcaaaa acgcaacttg
     9841 aaaaaatata tgttgctaag cgtgacagca gtcttccctt taaaggtttt gctgcagcaa
     9901 tgattgcaat gagtttctta tatttacaag gtggtagatt gattgatgtt ttaactgcga
     9961 tattagcagg tagtctagga tacctagtca ctgagatttt agatcgcaag ttacatgcac
    10021 agtttatccc agaattcatt ggttcattag ttattgggat tatcgccgtt attggacata
    10081 cacttatccc aacaggtgac ttggcaacta ttatcattgc agcagtcatg cctattgttc
    10141 ctggtgtatt aataacaaac gcaatacaag atttatttgg tggacacatg ttgatgttca
    10201 caacgaaatc attagaagca ttggttactg cgtttggcat cggtgctggc gttggtagcg
    10261 tattaatttt agtataggag tatcagacta tgttttggat cttaaacttt atctttagct
    10321 ttttagcttc aatgttcttc tgcgtcattt ttgatgcacc tagaaaatta tatctatcat
    10381 gtggtttcgt tggtacatgt ggatggatgg tttacacctt attcttcaac ggctttaatg
    10441 tgcacactat atactctagt ttctttggta gtttagcatt aggcttgtta agtcattata
    10501 tggctcgtaa acaaaaagaa cctgccatca tttttatggt aacgggtatc attccattag
    10561 tacctggtgg cttagcatac gatgctacaa aaaatttagt cttattaaat ttcagcacag
    10621 caataaatac aatgttagaa gtaacactta ttgcaggagc catcgcatta ggtttattat
    10681 tcgccgacca aatttctaaa ttaattgttt ctgggttcgt gaaatctttt aaacgattat
    10741 aaatattgtt agaattggct cagcccaaac tattagcaat tatatataag gttcagctgc
    10801 ctcgtcttaa atgtcaattt ataatacaaa aaatcgcacg atacgcctcc gaatgaagaa
    10861 ggtatattgt gcgattcttt tattatttag ttttttcttt ttcaggttct tcaatcatat
    10921 catcaacttc attttcactt ctatttaaaa tcaagcgact taattcatca ttatccatat
    10981 ttttcaattt aggatatcga atcacaaaga atattaaacc gatgattaac catccaagta
    11041 atgcaatata agacggtgca gtcagtgctg caggagaacc tggcactaat aacaacgcta
    11101 agaaaatgaa tgatacaaat gagccgataa tagcaaacgt tttgtaaacc ggtgcatacg
    11161 tattactttg tttgttataa ctgaataatt tcgctgcaga caaacatgta ataaagtagg
    11221 caatggatac acctgtagat gacatatcta caatccaagt caatgcagtt cttcctagcc
    11281 aaggtgcaat taacgacaca cctactagga atatgattgc gacatatggt gttttgtatt
    11341 tactatgtaa tttactaaac attgttggca taatacctga acgtcccata gaaaataaca
    11401 agcgacttga actcatcaag aatccattta aaccagtaaa tatacccatc ataattgcaa
    11461 ttgctaatac acctaatcca atataaccaa atgctgtttg tgtaacagca cctgttaacc
    11521 acaactgccc atttaaactt tgatgacttg ttgataacca accagtgtat aaaatcatga
    11581 caacataagt taatgatgct gctaataaac tgtacacgat aagcttaaat gtcttgtttg
    11641 gtgcaaagtt aaactcttct gctgtttgtg gaatattatc aaatccaaca tatgcccatg
    11701 gtgccacgga tacaataacc acaatagaca ctaaccatcc tttgctaggt tcagctaacg
    11761 gttgtaaatt ttcaagtgca aaattattac caaagaatga accaaagaac atcaataata
    11821 cgacgattac catcgccaca cagaaataat attgtaatga tccagataca cttgcgccac
    11881 gaatcgttac tagcatgaat acaagtagta atacggtcgc aataatgatt tccgtaatat
    11941 aaacgtccca gcccgcaatg gtgtatagtt tcccattatt taagacatct ggcaataaga
    12001 atttaactag taaactgaat gcggtcgcat ttaaagcaac gacacagaca taaccaaaag
    12061 ttaaaaacca tgatgagaag aaactcacat atctgccgaa acttaagaaa ctaaaggcaa
    12121 acgcgccccc tgatactgga aatctctcta ctaatgcgcc ataactaacc gcaattaata
    12181 tcattaataa tgtaccaata actataccaa ttgatgctgc aatcggacct gactgcttaa
    12241 tccagtctcc tggtaagatg aatgcgcccc atccgataca tgaaccatat gcaatcgccc
    12301 atacaaactt ttcagatagg ttttgtttta aatcgcctct atctatttgc ttattctttt
    12361 tttccataaa taaaaactca cctcgaaggt attctatacc caaaagatga gtttgtaact
    12421 ctttttcttg attattttta ttaaaaattt aatgtttagt tacataggta atctaaagtt
    12481 ttaaaattta accagcaaat ttgaatgtcg caatgattaa cattatccat ccaatgatga
    12541 acaatacgcc accaattggc gtaatcgcac ctaaaacttt aatttgagtt aatactaaaa
    12601 tatataatga tccactaaag aaaataatac cagcaaatat taaccagcca gcccagttaa
    12661 cattgattga agttgtacca ctaattacac ctataattaa taatgctaag ccatggtaca
    12721 tttgatacgt cgttgctttt tcccatactg ataaatagtg atcacttatt tttccttgta
    12781 aaccatgcgc accaaatgca cctgttccga cagccatcat cgcgtttaat gcacctaaaa
    12841 taataaataa tttcattgct acctctcctt atataattca gtattaataa atgtttaaat
    12901 ccgaacactc gaataaaagt atatgcgtct tgattattaa cctacatact taacaagcta
    12961 ttaagtaatt atgtttcatt gttgcttttt aaaaatcaaa tatggattca ccattgccaa
    13021 tgtcatcatc tgtcgctaat ctttgttgtt ggtttgaagg ttgtgaatat gacttattat
    13081 gatgatgtgc tgaatgcgta tttactttac cacccatcgc ttcaatttca gcagctgtca
    13141 cttgatgagt tggctgcgtt atttgtgatt gtggcatttg attatggtta gcaatacgac
    13201 gattcatttg ttgttcacca atatgtggtg tatttgatgt tgaagcatat aaagatgtta
    13261 atatatgtat ggcatacata tgtttttcaa agtcatggtc atgctgtgcc tgctctgctt
    13321 gtactaattc ttgctcaatt cgtgctatta aagtttctct attcaacatc tacgcctcac
    13381 tttcacacca attaattggt gattttccta ctgactctaa ataggcattc gctttggaat
    13441 acggttttga tccaaagaat cctctatatg cagacagtgg actaggatgc actgatttta
    13501 taatacaatg tttagatgta tcgataagct ttattttttg ctgtgcaggt ttcccccaca
    13561 aaataaagac aacatgttct ttataatcag acactgcttt aataatttca tcagtaaatg
    13621 tttcccaacc aatatcacga tgagaatttg cttcaccctg tcttacggtt aaaactgtat
    13681 tcaataacaa gacgccttct cttgcccaat cttgtaaatg cggtgtttgt ctaacgcatc
    13741 caatatcatc tgctaattct ttatacatat tacgtaaaga tggagggaat tttgcgttag
    13801 gttgcactga aaatgctaat ccatgtgctt ggtttggacc atgatacggg tcttgtccta
    13861 atataacaac tttgatattt tcaaacggtg ttaaatcaaa cgcttgatat atattttccc
    13921 tatcagggta tacgattgca gtcgaatatt ctttttctaa aaaatcatgc atagctttaa
    13981 agtcatgttt cgttgttatg tcatgaaaaa tttgcgacca ttccattcaa tctcacctca
    14041 ggacatattt taacatatca cacttatcta aagtgaatga cattttcaaa agttacaata
    14101 gtatgaatga ttcatttcaa caatcatcaa atattgaatg cttaatattt ttcaattagg
    14161 ttaaaaatga tatgatgaat acaatattat tatgtaggag tgaagctcaa tggctttaaa
    14221 gaaagtttta acaattgccg gttctgacac aagtgctggc gcaggtatgc aagcagattt
    14281 gaaaacgttc caagaattag atacgtatgg catggtcgct ttaactgcca tcgttactat
    14341 ggataaagat acatggtcac acgatgtcac accattacct atggacgtat ttgaaaagca
    14401 acttgaaacg gctttatcaa tcggacctga tgctattaaa acaggtatgt taggtactga
    14461 ggagataatc aaacgtgctg gagaagtata tgaggcatct aatgcacaat attttgtagt
    14521 agatcctgtc atggtttgta aaggcgaaga tgaagtgctt aatcctggaa atactgaggc
    14581 gatgattaaa tatttactac ctaaagcaac ggtagtgaca ccaaacttat ttgaagcggg
    14641 tcaattatca ggattaggta aattaaattc aattgaagat atgaaaaagg ctgcaacaat
    14701 tatctttgat aaaggcgcac agcatgtcat cattaaaggt ggcaaagctt tagatcaaga
    14761 taaatcgtat gacttatact atgatggtca aacattttat caactaacaa cagacatgtt
    14821 ccaacaaagt tataaccatg gtgctggttg tacatttgct gcggcaacaa ctgcatattt
    14881 agctaacggt aagtcaccga aagaagctgt gattagcgcg aaagctttcg tcgcttctgc
    14941 tattaaaaac ggttggaaaa tgaatgattt tgttggtcct gtggatcacg gtgcatacaa
    15001 ccgtattgaa catatcgatg ttgaagtaac agaggtttaa cgattaggcg gttcatctat
    15061 aaatcaacat tattagccac accacttaaa ttgggtatga actcaattta tgtgatgtgg
    15121 ttattttttt ataatcatca tgctctgtat ataatttaaa tttgaaagtt acatataagc
    15181 gttattagct tttattgaaa atttcataca tatcgaacaa tacatacaaa tatttcctat
    15241 acatctacat ctatgactaa ataacccttt attttcaaga tgaacttaaa ctcatctata
    15301 tgctcaatac ctctatcatt atcattcttt tgcgatagat gcctctctaa tcatatatgt
    15361 tat
//
LOCUS       DBNENW010000055        15294 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNENW010000055 DBNENW010000000
VERSION     DBNENW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.055"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(11..262)
                     /gene="rpsT"
                     /locus_tag="AC1LW8_001372"
     CDS             complement(11..262)
                     /gene="rpsT"
                     /locus_tag="AC1LW8_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIP4773214.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            608..2431
                     /gene="lepA"
                     /locus_tag="AC1LW8_001373"
     CDS             608..2431
                     /gene="lepA"
                     /locus_tag="AC1LW8_001373"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIP4773215.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            2998..4122
                     /gene="hemW"
                     /locus_tag="AC1LW8_001374"
     CDS             2998..4122
                     /gene="hemW"
                     /locus_tag="AC1LW8_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIP4773216.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            4223..5200
                     /gene="hrcA"
                     /locus_tag="AC1LW8_001375"
     CDS             4223..5200
                     /gene="hrcA"
                     /locus_tag="AC1LW8_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIP4773217.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5232..5858
                     /gene="grpE"
                     /locus_tag="AC1LW8_001376"
     CDS             5232..5858
                     /gene="grpE"
                     /locus_tag="AC1LW8_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIP4773218.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            5927..7759
                     /gene="dnaK"
                     /locus_tag="AC1LW8_001377"
     CDS             5927..7759
                     /gene="dnaK"
                     /locus_tag="AC1LW8_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIP4773219.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7895..9034
                     /gene="dnaJ"
                     /locus_tag="AC1LW8_001378"
     CDS             7895..9034
                     /gene="dnaJ"
                     /locus_tag="AC1LW8_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031072 - heat shock protein binding
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIP4773220.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            9038..9976
                     /gene="prmA"
                     /locus_tag="AC1LW8_001379"
     CDS             9038..9976
                     /gene="prmA"
                     /locus_tag="AC1LW8_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIP4773221.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            9978..10730
                     /locus_tag="AC1LW8_001380"
     CDS             9978..10730
                     /locus_tag="AC1LW8_001380"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIP4773222.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10737..12083
                     /gene="mtaB"
                     /locus_tag="AC1LW8_001381"
     CDS             10737..12083
                     /gene="mtaB"
                     /locus_tag="AC1LW8_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIP4773223.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12376..12552
                     /gene="rpsU"
                     /locus_tag="AC1LW8_001382"
     CDS             12376..12552
                     /gene="rpsU"
                     /locus_tag="AC1LW8_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIP4773224.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            12796..13479
                     /locus_tag="AC1LW8_001383"
     CDS             12796..13479
                     /locus_tag="AC1LW8_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIP4773225.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            13497..14486
                     /gene="floA"
                     /locus_tag="AC1LW8_001384"
     CDS             13497..14486
                     /gene="floA"
                     /locus_tag="AC1LW8_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HIP4773226.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            14503..15201
                     /locus_tag="AC1LW8_001385"
     CDS             14503..15201
                     /locus_tag="AC1LW8_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIP4773227.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPSLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
BASE COUNT         5689 a         2113 c         2934 g         4558 t
ORIGIN      
        1 tttaaaaaga ttatttattt gcagtcatta attgtgattt aatacggtca gctttgtttg
       61 aatgtattaa attactttga gcagctttgt ctactaactt aactgctaag cttactaatt
      121 catttttatt atcagcgtta tttgaaacag ctgttttagc gtttttaact gctgtacgca
      181 ttgcactctt ttgtgaaatg ttgcgtgctt cagctttttc agttgttttt acacgtttaa
      241 ttgcagattt gatatttgcc atttctgtca cctcctaaaa gtgagttcat cttatcaaaa
      301 ttttatttga ttacaacaag aaatatttta acaaaagtct aaccgttgtg caatcattta
      361 tttgatgggg ttagcttaaa attttgcaga tttaataaat tgtgttggaa acacaatcaa
      421 tacgttttat aattataagt acgtaattaa catattaaca tatcgcaagt atgtatttaa
      481 ataaagattg ttataatttc aaagttcatc caagattatg gcgtttgcat ttacctatta
      541 aaaacgttat tatatcaaag atgcgaaaga taatacgggt ttattttatg aaagtgagaa
      601 ggataaaatg gataatgagc aacgcttaaa aagaagagag aatataagga atttctcgat
      661 tatagcacat attgaccacg gaaaatctac attggctgat agaattttag aaaataccaa
      721 atcagttgaa acaagagata tgcaagatca gttactagat tcaatggatt tagaaagaga
      781 acgtggtatt acaatcaaat taaacgcagt tcgtttaaag tacgaagcta aagatggaaa
      841 tacttataca ttccatttaa tcgatacgcc tggacacgtc gattttacat atgaagtgtc
      901 acgttctttg gcagcttgtg agggcgcgat tttagtagta gatgcggctc aaggtatcga
      961 agcacaaaca ttagcaaatg tttatttagc attagataat gagttagagt tattgcctgt
     1021 tattaacaaa attgatttac ctgctgcaga acctgaacgc gtgaaacaag aaattgaaga
     1081 tatgataggt ttagaccaag acgatgttgt tttagcaagt gctaaatcta acattggaat
     1141 tgaagagata ctagagaaaa tagttgaagt tgtgccagct ccagatggcg acccagaagc
     1201 accactaaaa gcgttaatat ttgattctga gtatgatcca tatagagggg taatttcatc
     1261 gataagaatt gtagacggtg ttgttaaagc cggagataaa attcgaatga tggcgactgg
     1321 taaagagttc gaagtaacag aagttggaat taatacacct aagcagcttc cagttgatga
     1381 attaacagtt ggtgatgttg gttatattat tgcaagtatt aaaaatgttg atgattctag
     1441 ggttggtgac accatcacat tagctagtag acctgcatca gaaccattgc aaggttataa
     1501 gaaaatgaat ccaatggtat attgcggact gttcccaata gataacaaaa attataatga
     1561 tttaagagaa gcattagaaa aattacaatt gaatgatgca tcattagaat ttgagcctga
     1621 atcgtcacaa gcattaggtt ttggttatag aactggtttc ttaggtatgt tacacatgga
     1681 aataattcaa gaaagaattg aaagagaatt tggcattgaa ttaattgcaa cagcaccatc
     1741 tgtaatttat caatgtattt taagggacgg ttcagaagtg acggttgata acccagcaca
     1801 aatgccagat cgtgataaaa ttgataaaat atttgagcca tatgttcgtg caactatgat
     1861 ggttccaaat gactatgtag gtgcagtaat ggaattatgt caacgtaaac gtggacaatt
     1921 tataaatatg gactatttag atgatattcg tgtaaatatt gtttatgaat tacctttagc
     1981 tgaagttgta tttgatttct tcgatcaact taaatctaat actaaaggat atgcatcatt
     2041 tgattatgaa ttcatcgaaa ataaagaaag taatttagtt aagatggata ttttattaaa
     2101 cggtgataaa gtggatgcgc taagcttcat agttcataga gattttgcat atgaacgtgg
     2161 taaagcatta gttgaaaaac ttaaaacgtt aattccaaga cagcaatttg aagtacctgt
     2221 acaggctgca ataggacaaa aaattgtagc gcgtacaaat attaaatcaa tgggtaaaaa
     2281 cgttttagct aaatgttatg gcggtgacat aagccgtaaa cgtaaattac ttgaaaaaca
     2341 aaaagcaggt aaagctaaga tgaaagcagt tggtaatgtt gaaattccac aggatgcttt
     2401 cttggctgta ttgaaaatgg atgacgaata agttttttaa aaatttatta acaatttaca
     2461 atgaataaag tttaataact aaaaagaggg agcctaggat aaattaacgt cctgggcttt
     2521 acaatgttat attggcagcc atcgacagag ttaaaaatga gcttataaca atggggcccc
     2581 aacatagaag ctgacgaaaa gtcagcttac aataatgtgc aagttggggt ggggccccaa
     2641 catagagaaa ttggattgcc aatttctaca gacaatgcaa gttggggtgg gacaacgaaa
     2701 taaattttgc gaaaatatca tttctgtccc actcccttat gcatgagttt tactcatgta
     2761 attttatttt tatggacata ttacatctgg ctaatgtgta agagccacta cataataaat
     2821 cattagtggt tctttattat ttctatctca ctccctctaa acaagaataa atatcaaaat
     2881 gaatcgatat attagacaac cattgattaa acgttaaagt taaaagtaag aataattgca
     2941 gatagtccaa caggatatag ccgattggat aaacagtctg agaagcgggg cattaaaatg
     3001 acggtacaaa gtgcatatat acatattcca ttttgtgtaa gaatatgtac atattgtgat
     3061 ttcaataaat attttataca gaatcaacct gtagatgagt acttagatgc actaataaca
     3121 gaaatgtcta cagcaaaata taggaactta aagaccatgt atgtaggtgg cggcacacca
     3181 acggcccttt ctattaatca gttggaaaga ttacttaaag caatacgtga tacgtttaca
     3241 atcacaggcg agtatacatt tgaagcaaat cctgatgagt taactaaaga gaaagtccaa
     3301 ctattagaaa aatatggagt aaacagaatt tcaatgggcg ttcaaacatt caagccggag
     3361 ttattgtctg ttttaggtag aacgcacaat actgaagata tttacacttc ggtgttaaat
     3421 gctaaaaacg caggtattaa atcaatcagt ttagatttaa tgtatcattt accgaaacag
     3481 acgattgaag attttgaaca aagtttagat ctagctttag atatggatat tcaacatatt
     3541 tcgagttacg gcttaatact tgaacctaaa acccaatttt ataatatgta tagaaaaggg
     3601 ttgcttaaac ttcctaatga ggatttaggt gctgacatgt atcagttgct gatgtctaag
     3661 atagaacaat ctcctttcca tcaatacgaa atatctaatt ttgcattaga tggccatgaa
     3721 tcagaacata ataaggttta ctggtttaat gaggaatatt atggatttgg agcaggtgca
     3781 agtggttatg tagatggtgt gcgttatacg aatatcaatc cagtgaatca ttatatcaaa
     3841 gctataaata aagaaagtaa agcaatttta gtatcaaata aaccttcttt gactgagaga
     3901 atggaagaag aaatgtttct tggtttgcgt ttaaatgaag gtgtgagtag tagtaggttc
     3961 aaaaagaagt ttgaccaatc tattgaaagt gtctttggtc aaacaataaa taatttaaaa
     4021 gagaaggaat taattgtaga aaagaacgat gcgattgcac taacaaagcg agggaaagtc
     4081 ataggtaatg aggtttttga agctttccta ataaatgatt aaaaaaaatt gaaatttcga
     4141 gtctttaaca ttgacttact ttgaccaatt tgataaatta taattagcac ttgagataag
     4201 tgagtgctaa tgaggtgaaa acatgattac agataggcaa ttgagtatat taaacgcaat
     4261 tgttgaggat tatgttgatt ttggacaacc cgttggttct aaaacactaa ttgagcgaca
     4321 taacttgaat gttagtcctg ctacaattag aaatgagatg aaacagcttg aagatttaaa
     4381 ctatatcgaa aagacacata gttcttcagg gcgttcgcca tcacaattag gttttaggta
     4441 ttatgtcaat cgtttacttg aacaaacatc tcatcaaaaa acaaataaat taagacgatt
     4501 aaatcaattg ttagttgaga atcaatatga tgtatcatca gcattgacat attttgcaga
     4561 tgaattatca aatatatctc aatatacaac tttagttgtt catcctaatc ataaacaaga
     4621 tattatcaat aatgtacact tgattcgtgc taatcctaat ttagttataa tggttatcgt
     4681 attttcatca ggtcatgttg agcatgtaca tcttgcttca gacataccgt ttaataatga
     4741 caaactaaat acaatatcaa attttgttac aaacaaacta actgagttta atcagaattt
     4801 acaagatgat attgtctctt ttgttcaatc agagcaggaa gaaatattta tcaataaatt
     4861 gttaaatacg atgaataatc atatttctaa tcaaagtaat agcatttata tgggtggaaa
     4921 ggttaaactt attgatgcat tgaatgaaag taatgtatct tcaattcagc ccattctaca
     4981 atatatagaa tcaaatagaa ttgcagaatt attacaagat atatcttcac caaatattaa
     5041 tgtaaaaatt ggtaatgaaa ttgatgacag cttaagtgat atttcaattg taacaagtca
     5101 atatcatttt gatgaaacgt taaaaggtca aatcgctgtg attggaccta cagctatgca
     5161 ttatcaaaat gtcattcaat tattaaatag aatttggtaa tgatggatta gaaagtattg
     5221 gaggacagaa aatgacaaat aaagacgaat cagttgaaaa aaacactgaa tcaacagttg
     5281 aagaaacaaa cgtcaaacaa aatattgatg attcagttga acaagctgaa gaaagtaaag
     5341 gtcatttaca agatgaagca atagaagaaa cgtctgacga gaatgttatt gaagaaatag
     5401 atccaaaaga tcaaaaaatt aatgaacttc aacaattagc agatgaaaac gaagagaaat
     5461 atttaaggct ctacgctgag tttgaaaatt ataagcgtag aattcaaaaa gaaaatgaaa
     5521 taaataaaac atatcaagca caacgtgtgt taacagatat tttaccagca atagacaata
     5581 tagaacgtgc acttcaaatt gaaggtgatg atgagacttt taaatctctt caaaaaggtg
     5641 tacaaatggt gcatgaaagt ttgattaacg cactaaaaga taatggtctt gaagttatta
     5701 aaactgaagg tgaagcattt gatccaaata ttcaccaagc tgtagttcaa gatgataacc
     5761 ctgattttga atctggcgaa atcactcaag aactacaaaa aggatacaag cttaaagata
     5821 gagtattaag accatcaatg gtcaaagtaa accaataact taaatttggc gaaaagacat
     5881 tgtttaaaat taaattaatt taatgattaa atggaggaat tttattatga gtaaaattat
     5941 tggtatagac ttaggtacaa caaattcatg tgtaacagta ttagaaggcg atgagccaaa
     6001 agtaattcaa aaccctgaag gttcacgtac aacaccatct gttgtagctt tcaaaaatgg
     6061 agaaactcaa gttggtgaag tagctaaacg tcaagctatt acaaacccaa acactgttca
     6121 atctattaaa cgtcatatgg gtactgatta taaagtagat attgaaggta aatcatacac
     6181 accacaagaa atctcagcta tgattttaca aaacttaaaa aatacagctg aaagctattt
     6241 aggtgagaaa gttgacaaag ctgtaattac agtacctgca tactttaacg atgctgaacg
     6301 tcaagcaact aaagatgctg gtaaaattgc tggtttagaa gttgagcgta tcattaatga
     6361 accaacagct gcagcattag catatggttt agataaaact gataaagatg aaaaagttct
     6421 tgtttttgac ttaggtggcg gtacatttga cgtatctatc ctagaattag gtgacggtgt
     6481 attcgaagta ctatcaacag ccggtgacaa caaacttggc ggtgatgatt ttgaccaagt
     6541 aattattgac tacctagttg cagaattcaa aaaagaaaat ggcgtagact tatctcaaga
     6601 taaaatggca ttacaacgtt tgaaagatgc tgctgaaaaa gctaaaaaag acttatcagg
     6661 tgtatcacaa actcaaatct cattaccatt tatctcagct ggtgaaaacg gtccattaca
     6721 cttagaagta aacttaactc gttctaaatt tgaagaatta tcagattcat taattagaag
     6781 aacaatggaa cctacacgcc aagcaatgaa agacgctggc ttaacaaact cagatatcga
     6841 tgaagttatc ttagttggtg gatcaactcg tattccagca gtacaagaag ctgtcaaaaa
     6901 agaaatcggt aaagaaccta acaaaggagt aaacccggac gaagtagtgg caatgggagc
     6961 tgcaatccaa ggtggcgtta tcacaggtga cgttaaagac gtagtattat tagacgtaac
     7021 accactatct ttaggtattg aaattttagg tggacgtatg aatacgttaa ttgaacgtaa
     7081 cactacgatt cctacatcta aatctcaaat ctattcaaca gcagtagata atcaaccatc
     7141 agtagatgta cacgtattac aaggtgaacg tccaatggct gcggataata aaacacttgg
     7201 tagattccaa ttgactgata ttccaccagc tgaacgtggt aaacctcaaa ttgaagtaac
     7261 gtttgatatc gataaaaacg gtattgtaaa tgtaactgca aaagacttag gtacaaataa
     7321 agaacaaaga attacaattc aatcaagttc ttcattatca gacgaagaaa tcgaccgtat
     7381 ggtaaaagat gctgaagtta acgctgaagc agataaaaaa cgtcgtgaag aagtagactt
     7441 aagaaacgaa gctgacagtc tagtattcca agttgaaaaa actttaactg atttaggcga
     7501 aaatatcggt gaagaagata aaaaatctgc tgaagagaaa aaagacgctc ttaaaactgc
     7561 tttagaaggt caagatatag aagatattaa atctaaaaaa gaagaacttg aaaaagtgat
     7621 tcaagaatta tcagcaaaag tatatgagca agcggctcaa cagcaacaac aagcacaagg
     7681 tgcaaatgct ggtcaaaaca acgatagtac tgtagaagat gctgaattta aagaagtaaa
     7741 agacgacgac aaaaaataat taaacaatac aattagaatg ttgtcatgat ctgaaaagtc
     7801 aaagtcaata gaacattggc tttggctttt tatttggata gtatgacata ttacgttaaa
     7861 ctatatgagt taattaagaa aaggagagat agctgtggcc aaaagagact attatgaagt
     7921 tttaggcatt agtaaagatg cttcaaaaga tgaaatcaaa aaagcgtatc gaaagctttc
     7981 gaaaaaatat catccagata ttaacaaaga agaaggtgca gatgaaaagt ttaaagaaat
     8041 ttctgaagcc tatgaagttt taagtgatga taataaacgc gcaagctatg atcaatttgg
     8101 acatgatggt ccacaaggtt ttggtggaca aggattcaat ggctctgact ttggcggttt
     8161 tagtggcttc ggtggcggcg gttttgaaga tatctttagc tctttcttcg gtggcggcag
     8221 acaaagagat ccaaatgcac cgcaaaaagg tgatgatctt cagtatacaa tgacattaac
     8281 atttgaagaa gcggtatttg gtacaacaaa agaaatttca atccgtaaag atgtaacatg
     8341 cgaaacatgt catggtgatg gtgcaaagcc tggaacaagt aaaaagactt gtagttactg
     8401 taatggcgct ggtcatgtag ctgtagaaca aaacacaatt ttaggtagag ttcgtactga
     8461 acaagtttgt cctaaatgta atggaagtgg tcaagaattt gaagaagctt gtccaacatg
     8521 tcacggaaaa ggtactgaaa ataaaacagt taaattagaa gtgaaagtac ctgaaggtgt
     8581 cgacaatgaa caacaaatta gattagctgg tgaaggctct ccaggcgtga atggaggacc
     8641 tgctggtgat ttatacgtag tatttagagt taaaccatct gaaacattta aacgtgatgg
     8701 tgatgatatt tattacaaat taaatgttag cttcccacaa gcagctttag gcgatgaaat
     8761 aaaaatacca acattaaata atgaagttat gttaacaatc cctgctggta cacaaacagg
     8821 taagcaattc cgcttaaaag aaaagggtat aaaaaatgtt catggctatg gatacggaga
     8881 tttatatgtt gatattaaag tagtaacgcc tactaaatta acagatagac aaaaagaact
     8941 aatgaaagaa tttgcccaat taaatggtga agaaattaat gaacaacctt cgaattttaa
     9001 agatagagca aaaagattct ttaagggaga ataaagtatg aactggacag agctttcaat
     9061 tattattaat catgaagcag tagaattggc taccaatata cttgaaaatc atggatcaaa
     9121 tggtgtcgtg atagaagatt cagatgattt aattaaccaa ccagaagata aatacggtga
     9181 aatttacgct ttgaaaaaag aggattatcc agataaggga gtaagattga aagcctattt
     9241 taatgaaatg acttatgatg ataagttgcg acagcaaatt aaagatgagt tattaaattt
     9301 agatgaactt gatcaacata acgttcaatt cagtgagcaa attattgcag agacggattg
     9361 ggaaaatgaa tggaaaaact atttccatcc attccgagcg tcgaagaagt tcacaatagt
     9421 tcctagttgg gaaacatatg ctaaagaagc ggatgaagag ctttgcattg agctcgaccc
     9481 aggtatggct tttggaacag gtgatcatcc gactacaagt atgtgtttga aggcaataga
     9541 aacatatgta ttgccacagc attcagtaat tgatgttggt actggctcag gtatattaag
     9601 tattgcaagt catctaatcg gtgtaaaacg tattaaagcg ttggatattg atgaaatggc
     9661 agtgagtgta gctaaagaaa acttcagaag aaatcattgt gaaacgttaa ttgaagctgt
     9721 tccaggtaac ttattgaaag acgaaacaga aaaatttgat attgtaatag cgaatatttt
     9781 agcgcatatt attgatgaaa tgattgaaga tgcttataat actctaaatg aaggcggtta
     9841 ttttattact tctggtatta taaaagagaa gtatgaaggt atacagtcac atatggagcg
     9901 tgtaggtttt aaaattattt cagaacaaca tgacaacggt tgggtttgtc ttgttggcca
     9961 gaaagtgagt gaataatgtg caacgttatt tcatagacca aaacgctgat gtaagtcagc
    10021 gtttttttat tacaaaaaaa gaagatattc atcatataac aaatgtaatg agatatgatg
    10081 ttggtagtaa aattatttta acttttaatg atcaaacagt ttatttatgt gaaattgctg
    10141 atataacaag tgataaaatt gcagttgatt taaaagaaaa acaaaatatt aatacagaat
    10201 tgccagttga tgttacgatt tgcagtggac taatcaaagc tgacaaatat gagtggttgc
    10261 tacaaaaagc tactgaattg ggtgcttcat catttatagc tgtgagcatg gaacgttcaa
    10321 ttgttaaatt aaacgaggca aaagttgcca aaaagataga gcgttggcaa aaaataatta
    10381 aagaagctgc tgaacaaagc tatcgtttag tgattccatc tattcaattc gagtcgaatt
    10441 taaaattaat ttgtgatact attgataatt atgactatat tcttattgca tatgaagagg
    10501 aagcaaaaga tggtgagtta agcaatttca agcaaacttt acaacaattc aaggctcagg
    10561 ataaagtgtt gatgattttt ggtcctgagg gtggtttgtc agaaaatgaa atttcattat
    10621 ttagtaatac tagtacagtt gttggtcttg gaccgagaat tttacgggca gaaactgcgc
    10681 cattgtatgc gttaagtgca ataagttatg aaaaagaatt aatggggtga atatttatgt
    10741 caacagttgc gtttcacaca ttaggttgta aagtaaacca ttatgaaact gaagcaatct
    10801 ggcaattatt taaagaagca aactatgagc gcgttgactt tgaagcgaat gctgatgtat
    10861 ttgttattaa tacttgtaca gtaacgaata caggtgataa aaaaagtcgt caaataatta
    10921 gacgtgcaat aagacaaaat cctgatgctg taatctgtgt aacaggttgt tatgcgcaaa
    10981 cttcatcagc tgaaattatg gaaattcctg gtgtcgatgt agtagttggt acacaagata
    11041 gacataaact attaggttac attgatgaat tccgtaaaga acgccaacca attaatggtg
    11101 ttggaaatat catgaaaaat cgtaaatatg aagaattaga tgtcccatat tttacagata
    11161 gaacacgtgc gtcattaaaa attcaagaag gttgtaacaa cttctgcaca ttctgtatta
    11221 ttccatgggc tcgtggctta atgcgttcaa gagatccgga aaaagtagtt gaacaagcga
    11281 cgcaactagt gaattcagga tataaggaaa ttgtattgac gggaattcat acaggtggat
    11341 atggtcaaga tttaaaagat tataacttgg cccaattatt acgtgatctt gaaacgatta
    11401 atggattaga acgaattcga atttcttcaa ttgaagcaag tcaacttaca gatgaagtaa
    11461 ttgacgtttt agaacgttca acaaaagttg tgcgtcattt gcatattcca ttacaatctg
    11521 gttcagatac agtattaaaa cgtatgagac gtaagtatac aatggataga ttttcagaac
    11581 gattaacaaa attgcataaa gctttaccag acttggcagt tacgagtgat gtaattgttg
    11641 gtttcccagg tgaaactgaa gcagagttcc aagaaacata tgattttatc gtaaagcata
    11701 agttctctga actgcatgtt ttcccttatt ctcctagaat tggtacgcca gctgcaagaa
    11761 tggatgacca aattgatgaa gaaattaaaa atgaacgggt gcataagtta attacgctaa
    11821 gcaatcaact cggaaagtta tatgcgtcta aatttgatca agatgtgctt gaagtaattc
    11881 ctgaggaaca gggtgacaca gaaggcacat tagttggata tgcagataat tatatgaaag
    11941 tacaatttga aggtgacgaa tcactcatag gtcaaattgt aaaagttaaa attacgcaag
    12001 caaattatcc attaaatgaa gggcaagcaa ttaaagttgt tgatttcgca acaaataaat
    12061 ctgatagaga agttttagtt taagtattcc ttgattcata ccataaggtt ccgtattaaa
    12121 gatataggtt ttgtaacgtt gttttaacgg cttcacaatt tactatataa gcaactcttc
    12181 tcattaaata aattattaag agatttttag aacaaaatct aaaagtcttt gtgattttga
    12241 tgtgaaatat gtttcaaata aaaaacaatt tactaattga ccataaatta cagatatatt
    12301 atacttataa atgcatagtt ttactgtgca attgactata aagttccgtt gatatttgga
    12361 gggagggaaa tacagatgtc taaaacagta gtacgtaaaa atgaatcact tgaagatgcg
    12421 ttacgtagat ttaaacgttc agtttctaaa agtggaacaa tccaagaagt acgtaaacgt
    12481 gaattttacg aaaaaccaag cgtaaaacgt aaaaagaaat cagaagctgc acgtaaacgt
    12541 aaattcaaat aattaatacc tctgttgact ccctcaacac gaatattaat tatataaaac
    12601 aaacatcaca agttagtgtc ttacactaat atgtgatgtt tttttgttgt caatttttaa
    12661 ttaaaaaaag ttatatagtt tataaataat caaattgata ttctataggt tcttataact
    12721 ataaagtata ttcaatttca tgtataatta atgtgagggc gaggtgaaat tgtgagttat
    12781 aataattttt tacaaatgac aactatcttg gaatcaacgg ctggagatac ttgggttgaa
    12841 caagttagta atataattgt tcaacctatt tttacgttaa tattaacctg tttgacattc
    12901 ttaggatttg tatatcaact ttactctaaa aaaatcaatg cagctggtat tatcgccaca
    12961 ttatcattac ttattttatt tttgggattt ctaatccaag gaaatgtcaa tatgcattct
    13021 atcttaatat tctcaattgg cgttatatta gttgtaattg aattatttgt agttggtgca
    13081 gtaattggta ttattggcat gatactgata actataagca taacaacgct cggtgataat
    13141 ttgctattta tgcttgcgaa tgttatcgtt gccttgattt taacgattgt agaatgggtg
    13201 atattagtga agattttcaa cagaaagatt ccgtttttgg ataaagttat cttaaaagat
    13261 tcaactaatt ctgagtcagg ttacaattct catgataacc gctcgcacct cgtaggaaag
    13321 actgctcaaa cagttacaga tcttcgacct gcagggatta ttttttgtga aaatgaacgt
    13381 attgatgctg tttcagatgg caactttatt ttacgcaata aaacggtaaa aatccttgaa
    13441 gttgaaggaa caagagtagt tgtgagggaa gtagattaat taaaaggagc gataccatgt
    13501 ttagtttaag ttttatcgta atagcagtta ttatagtagt tgcattactt attttattct
    13561 catttgtacc cattggttta tggatttcag cgttagcagc tggcgttcat gttggtatag
    13621 gtacattggt tggtatgcgt ttacgtcgtg tatctccaag aaaagttata gcgccattaa
    13681 ttaaagcgca taaagcagga ctagcattaa caacaaacca attagaatcg cattatctag
    13741 caggaggaaa tgttgacaga gttgttgacg ctaatattgc tgcacaacgt gctgacattg
    13801 atcttccttt cgaacgtgct gctgcaattg accttgcagg acgtgacgta ttagaagcgg
    13861 ttcaaatgtc tgttaatcct aaagtcattg aaacaccatt tatcgcaggt gtagcaatga
    13921 acggtattga agtgaaagcc aaagctcgta tcacagttag agctaatatt gctcgacttg
    13981 ttggtggtgc tggtgaagaa acaatcatcg cacgtgttgg tgaaggtatc gtttcaacaa
    14041 ttggttctag taagcatcat acagaagtac ttgaaaaccc agataatatt tctaaaacag
    14101 ttttaagcaa aggtttagat tcaggtactg catttgaaat tttatcaatt gatattgctg
    14161 acgttgatat tagtaaaaat attggtgcag acttacaaac tgaacaagca ttagcagaca
    14221 aaaatattgc acaagcaaaa gctgaagaac gtagagctat ggctgtagca actgagcaag
    14281 aaatgaaagc gcgtgtacaa gaaatgcatg ctaaagtagt tgaagccgaa tctgaagtac
    14341 cattagctat ggctgaagca ttacgttcag gtaatatcag tgttaaagat tattataatt
    14401 tgaaaaatat cgaagctgat acaggcatga gaaatgcaat taataaacga actgatcaaa
    14461 gtgatgatga gtcacctgaa cattaagtcg agaggtgatt aaatgagtgt cggtattcta
    14521 atttttgtca tatcagtgat catttctatc attactacta tgcgcgaaaa tagtcataaa
    14581 gatagacaaa atcaaaagcc acctcaaaaa acatctaccg ataatgaacc aaaaaaaggt
    14641 ggcttttttg aagaaattga gcgaacgttt aaagaaataa gtgaagaatt aaatgaagaa
    14701 gaaaagaaat catcgaaacg aaaatatgat gatacgttac catctttatt cgatgaactt
    14761 ccaaaggaag agcctaaatc gaaacctgtt gtagaaccta tggcacctaa aaaacaacca
    14821 gaaacaaaac cgatgacaga gaaaccaatt acagtgccta aagcagaacc ggtggagcag
    14881 aaacatagac cttctagaca agataattct gacgaaatta gacgtcaatt agaaaaatca
    14941 cttagagatg atattaaaac gattcgtact gacattgata gagaaaaaga aaagcaaatt
    15001 gctaaaatgg aaaaacgtgc tagagatatt attgaggata aatacttatc tgaacgtaca
    15061 aaacgtttga aattaaagca gttgcttaat tctcaaaatg tcgaaaaaga tttgactaaa
    15121 tcagcgttcc aatttgataa agatgaagta atcaatggta tgatatggtc agaaatttta
    15181 gctaaaccaa aacaattata aaattttttg aaaacaagca ctatcgtaat ggtagttgct
    15241 tgttttttta cgttaagaaa aattgaaaaa caaagagaat ctttcgagaa atat
//
LOCUS       DBNENW010000056        14634 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNENW010000056 DBNENW010000000
VERSION     DBNENW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.056"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            206..715
                     /locus_tag="AC1LW8_001386"
     CDS             206..715
                     /locus_tag="AC1LW8_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HIP4773228.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(828..2018)
                     /gene="ltaA"
                     /locus_tag="AC1LW8_001387"
     CDS             complement(828..2018)
                     /gene="ltaA"
                     /locus_tag="AC1LW8_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid biosynthesis MFS flippase
                     LtaA"
                     /protein_id="HIP4773229.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(1996..3171)
                     /locus_tag="AC1LW8_001388"
     CDS             complement(1996..3171)
                     /locus_tag="AC1LW8_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HIP4773230.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3604..5088
                     /locus_tag="AC1LW8_001389"
     CDS             3604..5088
                     /locus_tag="AC1LW8_001389"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HIP4773231.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYSVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            5078..5329
                     /locus_tag="AC1LW8_001390"
     CDS             5078..5329
                     /locus_tag="AC1LW8_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HIP4773232.1"
                     /translation="MVNKRFIDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMTLDE
                     ETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            5329..6891
                     /locus_tag="AC1LW8_001391"
     CDS             5329..6891
                     /locus_tag="AC1LW8_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIP4773233.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     regulatory      7011..7118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            7193..7996
                     /locus_tag="AC1LW8_001392"
     CDS             7193..7996
                     /locus_tag="AC1LW8_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIP4773234.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKRPEIE
                     SPEAGDNHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            8215..10538
                     /locus_tag="AC1LW8_001393"
                     /pseudo
     CDS             8215..10538
                     /locus_tag="AC1LW8_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            10555..11913
                     /locus_tag="AC1LW8_001394"
     CDS             10555..11913
                     /locus_tag="AC1LW8_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIP4773235.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            12052..13563
                     /locus_tag="AC1LW8_001395"
     CDS             12052..13563
                     /locus_tag="AC1LW8_001395"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIP4773236.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLSKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYHISCSDNFIVKNR"
     gene            complement(13743..13831)
                     /locus_tag="AC1LW8_001396"
     tRNA            complement(13743..13831)
                     /locus_tag="AC1LW8_001396"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13794..13796),aa:Ser,seq:gct)
     gene            complement(13834..13908)
                     /locus_tag="AC1LW8_001397"
     tRNA            complement(13834..13908)
                     /locus_tag="AC1LW8_001397"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13874..13876),aa:Asn,seq:gtt)
     gene            complement(14050..14619)
                     /locus_tag="AC1LW8_001398"
     CDS             complement(14050..14619)
                     /locus_tag="AC1LW8_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HIP4773237.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         5208 a         2038 c         2693 g         4695 t
ORIGIN      
        1 cattatgagt aatttacatt agtagattta gtatgaattg tcttcatata gtttggtcta
       61 taatataatt tataaaagat tttactgttt gatttaattt aaatttgtcg aaattgcaaa
      121 agatgtataa tgaattattt ttaatgtaac ggttttcaaa gaaatttgat ataatagcaa
      181 taggttaaac aaaggaggaa ttcagatgat tttaggatta gcattaattc catcaaagtc
      241 atttcaagaa gcggtggatt cttaccgtaa aagatatgat aaacagtatt cacgaattaa
      301 accacatgtg acaattaaag cgccatttga aattgaagat ggtgatttag attctgtcat
      361 tgaacaggtt agagctcgta ttaatggtat accagcagta gaagttcatg ctacaaaagc
      421 ttctagcttc aaaccaacga acaatgtgat ttactttaaa gttgcgaaga cggacgactt
      481 agaagaattg tttaatcgct ttaatggaga agatttctat ggagaagctg aacatgtttt
      541 tgtgccacac tttacaatag cacaaggact atctagccaa gaattcgaag atatttttgg
      601 tcaagtagca ttagctgggg tagaccataa agaaattatc gatgaattaa ctttgttacg
      661 ttttgacgat gacgaagata aatggaaagt tattgaaacg tttaaattag cttaagtaac
      721 ataatagtat tgttaatcgt agtatgtttg aattaataag aaaatggtca tttttattga
      781 atgtaataaa aatgaccatt ttctttattt taaaatacgt tttaacctta cttagctttt
      841 tctctatttg ctataaagta gcttccataa aatacagcta agactaaaaa gattaatgcc
      901 gagaaataaa atgtattgtt taaattgttg gtaaattgtg taattaatcc gccgaataat
      961 ggccctatca ttgagccgaa tccttggata ctattaaaaa caccccaagt ttcttcttgt
     1021 tcatctgatt tgataaatcg tgccataaag gtattccatg ctggtaataa gatgccatac
     1081 attagaccga tagctaaagc gataatccac acgatgtgaa tattaacaat catagataga
     1141 gtgaaaatta atatcatgta taaaataaat ccgcttagaa taacaccata cataaagttt
     1201 ctgctgcggt tatctattag tttcgataaa aatagcatcg aaactgcaca gccgatacca
     1261 ccaataatga ttgcaacagt atattcaatt gtacttacgt taataacctt agtagcatat
     1321 gttggtaata taggaactag ggcagcaatt gcggcacctt gtaaaagaat accagggaac
     1381 aacaataaat ggcgctttgt cacatcaaca atttgtctca attgagcttt aactggacga
     1441 gtattataat ttgttaactt tacatcgaca aaataatata atatccatgc aattaaaacg
     1501 actaaagaca tcatgaaggc aaagcgtgtt gggtgcactt tgataagtag attcataaaa
     1561 accataccta ccaataggcc taacaaccat gaaaaataaa catagcccat ttgtttgcca
     1621 cgtttatctt cttcaacact ggataacata atgacccaaa taggactaac tgcaataccg
     1681 agcatcatag cactaaatat gattacaaaa ggtgatgctg gaaaccaaat aactaaaaat
     1741 aaacttgtaa atgctaaaat aaatccagtc gttaaaacga tttttgtgcc gaattttttc
     1801 agtaaaaatc ctataacaaa gtttgtagat gcatcagcaa taaaatgtat tgaaaatgct
     1861 agagacgtta ttgctacagc aatggatgta actgttggca agaaattaat atagcttagg
     1921 atatacatgc ctctcgcaaa ttccattaaa aataagataa taagcattaa aatgaaattt
     1981 ttatgattag cgtaattatt taacgaagaa tcttgcatat aaaggaacct ttccataaat
     2041 ctcttgtggt tgtgatgaat gaccgattaa atcaagtaag tctcgacata ttgtctgtgt
     2101 agcatactta attttatctt gttccattgt actaatcata ttagttaatt gctcattacc
     2161 gttcgttaaa cttgctacaa tttttattgc ttcttctgga gtatcagcga ttttaccaaa
     2221 acctttttct tcaaagtaaa gggcattttc aagctcttga ccaggtgcag gatttaggaa
     2281 aatcattgga atacaacggg cgaaaccttc agttattgtg ataccaccag gtttcgtaat
     2341 cataagttga cttgatgcca tccattcatt catgtgtttg gtataaccta gaatcaatac
     2401 attctcgtta gatttaaact tagctgttaa agaacgcttt agctctttgc tcttaccaca
     2461 aatcataact acttgtgcat ttgcactttt cgctaatata tcagtgatca tcgtgtcaaa
     2521 acctttagat acaccaaatg caccagctga cattaaaata gtttgcttat ctggatctaa
     2581 gttgttgtct attaaccact gcttttgatt aataggcgtt tcaaatttgt tatcaatagg
     2641 aatacctgtc actttaactg ttgaaggatc aatacctacg tctatgaagt cttgtttcgt
     2701 ttcttttgtt gccacataat atcttgttga atacggcgta atccagtttt tatgtaagcg
     2761 atagtctgtc atcactgtag caactggaat attaatgtta aattgctcag tgagtaccga
     2821 cataactggt gtaggaaacg ttaataatat taaatctggc ttttctttta tcaataaatt
     2881 gattaactta ttaagtccat agtatttgta aaaacatttg tctagtttat ctgggcggct
     2941 gtaataaaac cctttgtaca tatttctaaa atatttaaag ctattgatat accatttttt
     3001 acaaatagaa gtcaaaattg gatgagcttc cataaataaa tcgtgctcaa tgacgcttaa
     3061 atggtctaga ttcatatcat taagttgatt aacgatactc tgtgtaactt gcatatgacc
     3121 gttaccgaat gagccagtaa taatcaatat ctttttattt tgagtaacca ttaatagcca
     3181 ccctccatta gtttgaaaat tttatttaag tgtaacttat tttacggcat tataaaagaa
     3241 ataaagacgc aaagtcgtta catttatagc aattttaatc aatagatgaa ttgatacaaa
     3301 ataaaacgtt attttataaa gcaatttatt gttctatgtt ttatttgtat atttaaaatt
     3361 atccagtata caattatagc atatttttgg aaacaattat gatattatac catgttacaa
     3421 gatggtttta ataatttaag atgagccata attgtgaaac taattcataa taacgtatgt
     3481 tttattttta atagtagaaa ttagaaaatg ctgattagta ggatataaca gtgaaattat
     3541 aaatttatta acatcaacaa aacgtgtata ataaacatat tgtagaaaaa ggagcggttc
     3601 agtttggatg caagtacgtt gtttaagaaa gtaaaagtaa agcgtgtatt gggttcttta
     3661 gaacaacaaa tagatgatat cactactgat tcacgtacag cgagagaagg tagcattttt
     3721 gtcgcttcag ttggatatac tgtagacagt cataagttct gtcaaagtgt agctgatcaa
     3781 gggtgtaagt tggtagtggt caataaagaa caatcattac cagctaacgt aacacaagtg
     3841 gttgtgccgg acacattaag agtagctagt attctagcac acacattata tgattatccg
     3901 agtcatcagt tagtgacatt tggtgtaacg ggtacaaatg gtaaaacttc tattgcgacg
     3961 atgattcatt taattcaaag aaagttacaa aaaaatagtg catatttagg aactaatggt
     4021 ttccaaatta atgaaacaaa gacaaaaggt gcaaatacga caccagaaac agtttcttta
     4081 actaagaaaa ttaaagaagc agttgatgca ggcgctgaat ccatgacatt agaagtatca
     4141 agccatggct tagtattagg acgactgcga ggcgttgaat ttgacgttgc aatattttca
     4201 aatttaacac aagaccattt agattttcat ggcacaatgg aagcatacgg acacgcgaag
     4261 tctttattgt ttagtcaatt aggtgaagat ttgtcgaaag aaaagtatgt cgtgttaaac
     4321 aatgacgatt cattttctga gtatttaaga acagtgacgc cttatgaagt atttagttat
     4381 ggaattgatg aggaagccca atttatggct aaaaatattc aagaatcttt acaaggtgtc
     4441 agctttgatt ttgtaacgcc ttttggaact tactcagtaa aatcgcctta tgttggtaag
     4501 tttaatattt ctaatattat ggcggcaatg attgcggtgt ggagtaaagg tacatcttta
     4561 gaaacgatta ttaaagctgt tgaaaattta gaacctgttg aagggcgatt agaagtttta
     4621 gatccttcgt tacctattga tttaattatc gattatgcac atacagctga tggtatgaac
     4681 aaattaatcg atgcagtaca gccttttgta aagcaaaagt tgatattttt agttggtatg
     4741 gcaggcgaac gtgatttaac taaaacgcct gaaatggggc gagttgcctg tcgtgcagat
     4801 tatgtcattt tcacaccgga taatccggca aatgatgacc cgaaaatgtt aacggcagaa
     4861 ttagccaaag gtgcaacaca tcaaaactat attgaatttg atgatcgtgc agaagggata
     4921 aaacatgcaa ttgacatagc tgagcctggg gatactgtcg ttttagcatc aaaaggaaga
     4981 gaaccatatc aaatcatgcc agggcatatt aaggtgccac atcgagatga tttaattggc
     5041 cttgaagcag cttacaaaaa gttcggtggt ggccctgttg gtcaataaaa gatttattga
     5101 tgaaggtaaa actattgatg tttatttatt cgaagcatta aatgaccaga taatcattgc
     5161 tataccagat tggttttggt catatcagat ggcaatgaca ttagatgaag aaacttgttt
     5221 tgaagcaata ctcatgcaat tgtttgtttt taaagaagag gaagaggcag aatcgattgc
     5281 atcacaacta acagattgga tagaaacata taaaaaggag aaagactaat gaacttaaag
     5341 caagaagttg agtctagaaa gacttttgcg attatttcac atcccgatgc agggaaaaca
     5401 acgttaactg aaaaactatt gtacttcagt ggtgccattc gtgaagcggg tacagttaaa
     5461 gggaagaaga ctggtaaatt tgcgacaagt gactggatga aagttgaaca agaacgtggt
     5521 atttctgtaa caagttcagt aatgcaattt gattacgatg attataaaat caatatctta
     5581 gatacaccag gacatgaaga cttttcagaa gatacgtata gaacattaat ggcagttgac
     5641 agtgctgtca tggtcataga ctgtgcaaaa ggtattgaac cacaaacatt gaagttattt
     5701 aaagtttgta aaatgcgtgg tattccaatc tttacattca ttaataaatt agaccgagta
     5761 ggtaaagaac catttgaatt attagatgaa atcgaagaga cattaaatat tgaaacatac
     5821 cctatgaatt ggccaattgg tatgggacaa agtttctttg gcatcattga tagaaagtct
     5881 aaaacaattg aaccatttag agatgaagaa aatatattac atttgaatga tgattttgag
     5941 ttggaagaag atcatgcaat tacaaatgat agtgcttttg aacaagcgat tgaagaatta
     6001 atgttggttg aagaagcggg tgaagccttt gataatgacg cgctgttgag tggagactta
     6061 acacctgtat ttttcggttc agctttagct aactttggtg tacaaaattt cttaaatgca
     6121 tatgttgatt ttgcgccaat gccaaatgca agacaaacaa aagaagaggt tgaagtaagc
     6181 ccgtttgatg attcattttc aggatttatc tttaaaattc aagccaacat ggaccctaaa
     6241 caccgtgata gaattgcatt tatgcgtgtc gttagtggtg catttgaacg tggtatggat
     6301 gttactttgc aacgtactaa taaaaagcaa aagatcacac gttcaacgtc atttatggca
     6361 gacgataaag aaactgtgaa tcatgctgta gcaggcgata tcattggact atatgatact
     6421 ggtaattatc aaattggaga tactttagtt ggtggaaaac aaacctacag tttccaagat
     6481 ttaccacaat ttacgccaga aatttttatg aaagtttctg ctaaaaacgt catgaaacag
     6541 aagcatttcc ataaaggtat tgaacaatta gtacaagaag gtgcgattca atactataaa
     6601 acattacaca caaaccaaat tattttaggt gctgttggtc agttacaatt tgaagttttc
     6661 gaacatagaa tgaaaaatga atataatgtt gatgttgtta tggagccagt aggccgtaaa
     6721 attgcacgtt ggattgaaaa tgaagaccaa attacagata agatgaacac atcaagatcg
     6781 attttagtga aagatagata tgacgattta gtattcttat ttgaaaatga atttgcaaca
     6841 agatggtttg aagagaaatt ccctgaaatt aaattgtata gtttacttta acagctcaat
     6901 tgtataatcg aatttgttac attaaaaata attgtttcgt tgaagaaaaa taaattgtat
     6961 attttaaaag aaaaaggtat actatgatgt atcaaatgaa taacctatgg cattttgtca
     7021 gaggggagta acttaagaat catgaccgta taaatgattc gacactttat cgtcattacg
     7081 aagatatctt ccggtaaagt gggcaattta aattgcttag tgagaccttt gctatttatt
     7141 tagcataggt ctttttgttt gtacttaact tatttattta aaggagttgt acatgttaat
     7201 ggatccaagt ttgatcttac cttatttatg ggtacttgtc gttttagtat ttttagaagg
     7261 cttattagca gcagataacg cgattgttat ggctgtaatg gttaagcact taccacccga
     7321 acaacgtaaa aaagctttgt tttacggttt gttaggtgca tttgtattta gatttttagc
     7381 attattctta attagtatta tcgcgaactt ttggtttatt caagctgcag gagcggttta
     7441 cttaatttat atgtcaatca aaaatctgtg gcagttcttt aaacgcccag aaattgaaag
     7501 tcctgaagct ggagataatc atcattatga tgaatctggt gaagagatta aagcaagtaa
     7561 caaatcattc tggggaactg tgttgaaaat agaatttgca gatatcgcat ttgccattga
     7621 ttctatgctt gctgctttag ctattgctgt aacacttcct aaagttggta ttcactttgg
     7681 tggtatggac ttaggtcagt tcgtagtcat gttcctaggt ggaatgattg gtgttattct
     7741 aatgcgttat gcagcaacat ggtttgtaga gctattaaac aaatatccag gacttgaagg
     7801 tgcagccttc gcgatcgttg gttgggtagg tgttaaatta gttgtcatgg tattagcgca
     7861 cccagacatc gctgtattgc ctgagcactt cccacatggc gtattatggc aatctatttt
     7921 ctggacagta ctaattggat tagtaattat cggttggtta ggttcagttg ttaaaaataa
     7981 aaaatcgcat aaataattga tgtgaagcgg acaatcttaa tttagtttaa ggttgtcctt
     8041 tttcatttaa ttgagtgatt tatgaaaaat ggattttgaa gaatgtgaat caaaagatgc
     8101 gatatagtat taagaaaatg tgccttttat atttagcatt ttttcaatag aaattatata
     8161 gattttaaag caaattaggt gttaatgtgt cataatgata agtgatttta ttgaatggag
     8221 tggacattag tggatattgg taaaaaacat gtaattccta aaagtcagta ccgacgtaag
     8281 cgtcgtgaat tcttccacaa cgaagacaga gaagaaaatt taaatcaaca tcaagataaa
     8341 caaaatatag ataatacaac atcaaaaaaa gcagataagc aaatacataa agattcaatt
     8401 gataagcacg aacgttttaa aaatagttta tcatcgcatt tagaacagag aaaccgtgat
     8461 gttaatgaga ataaagctga agaaagtaaa agtaatcagg atagtaagtc agcatataac
     8521 agagatcatt atttaacaga cgatgtatct aaaaaacaaa attcattaga ttcagtggac
     8581 caagatacag agaaatcaaa atattatgag caaaattctg aagcgacttt atcaactaaa
     8641 tcaaccgata aagtagaatc aactgacatg agaaagctaa gttcagataa aaacaaagtt
     8701 ggtcatgaag agcaacatgt actttctaaa ccttcagaac atgataaaga gactagaatt
     8761 gattttgagt cttcaagaac tgattcagac agctcgatgc agacagagaa aataaaaaaa
     8821 gacagttcag atggaaataa aagtagtaat ctgaaatctg aagtaatatc agacaaatca
     8881 aatacagtac caaaattgtc ggaatctgat gatgaagtaa ataatcagaa gccattaact
     8941 ttgccggaag aacagaaatt gaaaagacag caaagtcaaa atgagcaaac aaaaacctat
     9001 acatatggtg atagcgaaca aaatgacaag tctaattatg aaaatgattt aagtcatcat
     9061 atgccatcga taagtgatga taaagataac gtcatgagag aaaatcatat tgttgacgat
     9121 aatcctgata atgatatcaa tacaccatca ttatcaaaaa cagatgacga tcgaaaactt
     9181 gatgaaaaaa ttcatgttga agataaacat aaacaaaatg cagactcgtc tgaaacggtg
     9241 ggatatcaaa gtcagtcaag tgcatctcat cgtatcactg aaaaaagaaa taatgctatt
     9301 aatgaccatg ataaattaaa cggtcaaaaa ccaaatgcaa agacatcggc aaataataat
     9361 caaaaaaggc tacatcaaaa ttgaacaaag ggcgcgctac aaataataat tatagtgaca
     9421 ttttgaaaaa gttttggatg atgtattggc ctaaattagt tattctaatg ggtattatta
     9481 ttctaattgt tattttgaat gctattttta ataatgtgaa caaaaatgat cgcatgaatg
     9541 ataataatga tgcagatgct caaaaatata cgacaacgat gaaaaatgcc aataacacag
     9601 ttaaatcggt cgttacagtt gaaaatgaaa catcaaaaga ttcatcatta cctaaagata
     9661 aagcatctca agacgaagta ggatcaggtg ttgtatataa aaaatctgga gatacgttat
     9721 atattgttac gaatgcacac gttgtcggtg ataaagaaaa tcaaaaaata actttttcga
     9781 ataataaaag tgttgttggg aaagtgcttg gtaaagataa atggtcagat ttagctgttg
     9841 ttaaagcgac ttcttcagac agttcagtga aagagatagc tattggagat tcaaataatt
     9901 tagtgttagg agagccaata ttagtcgtag gtaatccact tggtgtagac tttaaaggca
     9961 ctgtgacaga aggtattatt tcaggtctga acagaaatgt tcctattgat ttcgataaag
    10021 ataataaata tgatatgttg atgaaagctt tccaaattga tgcatcagta aatccaggta
    10081 actcgggtgg tgctgtagtc aatagagaag gaaaattaat tggtgtagtt gcagctaaaa
    10141 ttagtatgcc aaacgttgaa aatatgtcat ttgcaatacc tgttaatgaa gtacaaaaga
    10201 ttgtaaaaga tttagaaaca aaaggtaaaa ttgactatcc cgatgtaggt gttaaaatga
    10261 agaatattgc cagtctaaat agttttgaaa gacaagcagt taaattgcca ggaaaagtta
    10321 agaacggtgt tgttgtagat caagttgaca acaatggttt agcagatcaa tctggtctga
    10381 aaaaaggtga tgtaattact gaattagatg gcaaactttt agaagatgat ttacgcttta
    10441 ggcagattat atttagtcat aaagatgact tgaaatcaat tacagcgaag atttatagag
    10501 atggtaaaga gaaagaaatt aatattaaac taaaataatg ttgaggtgaa caacgtgtca
    10561 atttttagcc agtttttaaa aagatcaagc cctcaacaag gtattgtatt gtactatatc
    10621 gtcgcaattg tcattgcatt tttattatta aacttaccgt atgttcataa accaggtgta
    10681 gaagtaaatc caattgacac attatttgtt gccgtatccg gaattagtgt tactggattg
    10741 tctccgataa gtattgtcga tacctattct acatttggac aattaattat cctcgtgata
    10801 ttaaatattg gtggaattgg cgtcatggca attggtacga tgttatgggt ggtactaggt
    10861 aaacatattg gaattagaga acgtcagtta attatgttag ataataacaa aaacacaatg
    10921 agtggtaccg tcaaattgat tattgatatt gtaaaatcaa tatttgtaat cgaactcgta
    10981 ggagccatgt tattagcatt ttacttttat cgagataatc cagatttaaa atatgcaatc
    11041 atgcaaggtg tttttgtttc tatttctgcc actaccaatg gtggattaga tattacaggt
    11101 aagtcattaa ttccttatgc acatgattat tttgtacaag cgatagttat atttttaata
    11161 attttaggat caatcggctt cccagtatta ttagaagtta aagcttatat tcaaaatagg
    11221 gttactaatt ttagattttc attatttact aaaattacga catcaacata tttattccta
    11281 tttattgttg gggtattagc cattctatta tttgaacata accatgcgtt caaaggatta
    11341 agttggcatc aatcgttatt ctattcgctg tttcaatcag cgactacaag aagtgcgggt
    11401 cttcaaacaa ttgatgtgac aacactaagt gaccccacta atattatcat gggtatttta
    11461 atgtttatag gatcttcgcc aagttcggtt ggtggcggta ttcgtacaac aactttcgct
    11521 attttaattt tgtttttaat taactttagt aataatgccg ataaaacatc cattaaagtt
    11581 tacaatagag aagtacacat tatggatatt caacgttcat ttgcagtatt tacaatggcg
    11641 acaattttaa catttttagg aatgctaatt atatcagcta ctgaaaatgg taagcttaca
    11701 tttttacaag tattttttga agtcatgtct gcatttggaa cttgtggact atcgcttggt
    11761 gtcacaagtg atattagtga tatttctaag gtcgtactaa tgatactcat gtttatagga
    11821 cgtgttggct taatatcatt tatcattatg atagcaggac gtcgagaacc agataaattc
    11881 cattatccaa aagaacgtat tcaaatagga taatataata gcaatctaag tttagttaat
    11941 gtagatttta actggaactt agattgcttt tttagtttgt atttttaact tattttataa
    12001 gacgattggt ttcgaaaatg gtaaactagt aacaatgaga ggtgtaacat gatggaaaaa
    12061 aatgaaaaca ttaatgtaga gattttaact acgtcagata tgcatagtca tttcttaaat
    12121 ggtgattatg gttcaaatat ttatagagct ggtacttatg ttaaccaagt aagagcacaa
    12181 aatcatcgcg tcattttatt agatagtggc ggaagtttag ctggctcgtt agcggcctat
    12241 tattatgcta ttgttgcacc ttataaacga catccaatga taaagttaat gaacagaatg
    12301 cattatgatg ctagcggtgt gagtccaagt gaattcaagt ttggtttatc atttttaact
    12361 cgttcaattg ctttggcacg ttttccatgg ttatcagcaa atattgaata caatgttact
    12421 aaggagcctt atttttcaac tccatattgt attaaacatt ttggtgactt aaaaattgct
    12481 atcgtaggcg ttacagcaga tggtttaatg gaaaatgagt attctgaaat ggagcaagat
    12541 gtatctattg aaaagacatt agtggcatca aaacgatgga ttagatatat ccatgaagtt
    12601 gaagagccgg attttttgat tgtaatttat catggtggat tgaataaaat tagtaatagt
    12661 acgaaaaata aaaaggcaag ttcgaatgaa gctgaaaaat taatggaaga actcggtgtt
    12721 atagatttaa tgattacagc tcatcagcat caaacaatag tcggtcaaga tcatgaaacg
    12781 tattatgttc aggctggtca agatgcaaaa gagcttgtac atctttcgat taattttaaa
    12841 aagagaacaa caacttatga tgttgaaagc attgattcta aagtgattga cttaaatgag
    12901 tatgaagagg accaagaatt attagattta acattctatg atagaaaagc agtggcttat
    12961 tggtcacagg aaatcataag tgataaaggt ttgatgttat cagtaaatgg gttacaagat
    13021 ttagtctgtc aaacacatcc attttcacaa ttattacatg atgcaattca ccttgcattt
    13081 gataatgata taacatgtgt ccacgtgcct atgaacggag agaaggggtt gagtggacag
    13141 attcgaaatg aagatttgta tcatgcatac ccatatccag ataagccaat ggatatgaca
    13201 attagtggtc aaaatatcaa agatatattg gagtatagtt attcacattt agattttgtt
    13261 aacgagcaat taagcttaac aattattgat gaaacgttat gtacaatgtg gcaaggattc
    13321 aattatgaga ttgatatgaa tcaagaacct gggcaacgag taatgttaga tcaaattgat
    13381 ttgagtaaga gttatagagt tacaatgact gactattgtt atcgtaacta caagaattat
    13441 ttaaaaaatg ctattataca tgaatcatac gatgaaacaa tgagtacatt aattgcagag
    13501 aagttaagag atccgaatta tcatatttca tgtagtgata attttatagt taaaaacagg
    13561 taaatattgc tatgtaataa attaattata agttcaacaa gtataaaaac ctttcttaga
    13621 gaagattatc taggaaaggt ttttagaatt ttaaaattga tataaaagaa cagtgaaatt
    13681 ctcattcatt atatataagt aaacaaaaaa gctaaaatgg tatataccat tttagcaagt
    13741 aacggaaacg gagggattcg aaccctcgcg ccgctttcgc gacctacacc cttagcaggg
    13801 gcgcctcttc agccaacttg agtacgtttc caatggctcc acaggtagga ctcgaaccta
    13861 cgaccgatcg gttaacagcc gatagctcta ccactgagct actgtggatt aatttgtatg
    13921 tgtttgaata tcaagcacaa atattattat aacaagatta agttaatttg caatagatac
    13981 cttaaaaaaa tatgcagaac atttacacta tatttataaa acattccgca tattttaaaa
    14041 ataaacaatt tatttatctt caaataaaga atagcgtgtc aatgcttcaa acacattcaa
    14101 tgtggcttta ttataatcta ttggttggtc gggatttttt gatatcatgc gagattgttt
    14161 atctaccatg taatagtaaa aaatggaatt gttatattga tgccataaac tgtggtatga
    14221 aacatgaaga atgattgatt cattattaat aaatgtcact ttacatttac gattttttaa
    14281 ttctttaata ctttcgatat aatgcatatt taaccaaata ttttcatttt gtctgtcaga
    14341 atgtgttggg aaaaaataag ttggaaataa tggtgttagt aaaataggtg gtttactaga
    14401 aatgccagta atgcggtttg tctctgcttt cttgccaaga taagtattac cataaaattt
    14461 gcaagagcgt tcaatgattt tctggacttt aaaaggattt tgaatacgcg ttttgtcaaa
    14521 ccgaattatt tcactaccgt ttctttgatc gtcatcatca aatgctggtc gaataaccat
    14581 gtctcctttg cgtatcacat aaatattttg agaatacata agcaaaacct cgct
//
LOCUS       DBNENW010000057        14608 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNENW010000057 DBNENW010000000
VERSION     DBNENW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.057"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(93..404)
                     /locus_tag="AC1LW8_001399"
     CDS             complement(93..404)
                     /locus_tag="AC1LW8_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP4773238.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            complement(426..2849)
                     /gene="leuS"
                     /locus_tag="AC1LW8_001400"
     CDS             complement(426..2849)
                     /gene="leuS"
                     /locus_tag="AC1LW8_001400"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIP4773239.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEAEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDE
                     YKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYG
                     TGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEA
                     AITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPL
                     LLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYID
                     PKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKL
                     FNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLD
                     GSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQ
                     LMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEA
                     LLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVP
                     QKLVNIVAK"
     gene            complement(3137..4318)
                     /locus_tag="AC1LW8_001401"
     CDS             complement(3137..4318)
                     /locus_tag="AC1LW8_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIP4773240.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTQLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            4428..5381
                     /locus_tag="AC1LW8_001402"
     CDS             4428..5381
                     /locus_tag="AC1LW8_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIP4773241.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            5378..5941
                     /locus_tag="AC1LW8_001403"
     CDS             5378..5941
                     /locus_tag="AC1LW8_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4773242.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(6061..6462)
                     /locus_tag="AC1LW8_001404"
                     /pseudo
     CDS             complement(6061..6462)
                     /locus_tag="AC1LW8_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator, SarA/Rot family"
     gene            complement(7036..7863)
                     /locus_tag="AC1LW8_001405"
     CDS             complement(7036..7863)
                     /locus_tag="AC1LW8_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP4773243.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(7866..7985)
                     /locus_tag="AC1LW8_001406"
     CDS             complement(7866..7985)
                     /locus_tag="AC1LW8_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773244.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            8097..9098
                     /locus_tag="AC1LW8_001407"
     CDS             8097..9098
                     /locus_tag="AC1LW8_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIP4773245.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(9220..9684)
                     /gene="ribH"
                     /locus_tag="AC1LW8_001408"
     CDS             complement(9220..9684)
                     /gene="ribH"
                     /locus_tag="AC1LW8_001408"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIP4773246.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(9697..10878)
                     /gene="ribB"
                     /locus_tag="AC1LW8_001409"
     CDS             complement(9697..10878)
                     /gene="ribB"
                     /locus_tag="AC1LW8_001409"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIP4773247.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(10889..11521)
                     /locus_tag="AC1LW8_001410"
     CDS             complement(10889..11521)
                     /locus_tag="AC1LW8_001410"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIP4773248.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(11528..12571)
                     /gene="ribD"
                     /locus_tag="AC1LW8_001411"
     CDS             complement(11528..12571)
                     /gene="ribD"
                     /locus_tag="AC1LW8_001411"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIP4773249.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKITKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKKPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(12698..12833)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13052..14554)
                     /locus_tag="AC1LW8_001412"
     CDS             complement(13052..14554)
                     /locus_tag="AC1LW8_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIP4773250.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVYRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHKVSQLYDALESNEQQQRSDNQ"
BASE COUNT         4747 a         2737 c         2045 g         5079 t
ORIGIN      
        1 cagaaagtaa ttcataccaa aaaacaccaa gcaaccgaaa cgttacttgg tgtcattaca
       61 aatgtaatat tattttaaat tatgtcacta ctttaaatac tttttatttc caaaccttca
      121 tcgccccatg cgtgcatgcc gccttcgaca tttacggcat caatgccatt tgcctctaaa
      181 tattctacaa ctttagcgct tcgaactcca ccagcacata caatataata tatttcattt
      241 ttattaaatg aatttaaatt atccggaatg gtatccattg gaatcaactt tgcattagga
      301 atatatccca ttgctgtttc ttcgtcagta cgaacatcaa caatttgaac tggtttagat
      361 tctaaaagtt tgttttttaa ttcatctgta gtaattgact tcatttcaaa gtcctcctta
      421 aaacattatt tagctacaat attgactaat ttttgaggaa cagcgatgac tttcatgatg
      481 tctttacctt caatactcgc tttaacattg tcattagata aggcaatttc ttgcatttct
      541 tcttttgatg tatctttagc aattttaatt ttagctctca atttaccatt tacttgaaca
      601 acgatttcta cttcatcatc tacaagtagt gcttcgtcat aagttggcca aggttggtac
      661 gtaatagact cttcatgtcc taattttgac cataattctt caccgatatg tggtgcaata
      721 ggtgctaaca ttttaacgaa gccttcaatg taaggtttat aaacttcatc aactttataa
      781 cactcattaa taaataccat taattgacta atagcagtat taaatcctaa tgtttcaaag
      841 tcttctgtta cctttttaac agtttggtta taaactttat ctaaagattt attatttgta
      901 gttacaattt ttgaactcaa tgttccatct tcatttacca ttaaacgcca tacgcgatct
      961 aagaatcgac gagacccatc taatcctttt tcactccatg caattgcagc atctaaaggt
     1021 cccataaaca tttcgtaaag acgtaaagta tctgcaccat gagactgtac tatatcatca
     1081 ggattgatta catttccttt agatttactc atcttctcat taccttctcc taaaatcata
     1141 ccttggttaa ataatttttg gaaaggttct ttagtaggta cgatacccaa atcataaagg
     1201 actttatgcc aaaatcttgc atataataag tgaagaaccg catgttctac tccaccgata
     1261 tataaatcaa caggtaacca atgttttaat ttttcaggat ctgctaacat attttcattt
     1321 ttaggatcga tgtaacgtaa ataataccaa caactacctg cccattgtgg cattgtattt
     1381 gtttcacgac gtcctttcat acctgttttt tcatctacaa catttacaaa tgaatcaata
     1441 ttagctagtg gagactcacc agtccctgat ggcttgattt catctgtttc aggtaacaac
     1501 aatggtagct cttcttcagg aacagttgtc attgttccat cttcccaatg aatgacagga
     1561 attggttcgc cccaataacg ctgacgactg aataaccaat ctcttaattt gtaattaact
     1621 ttcttttcgc cagcaccttt ttgctctaat aattgaatag ctttagtaat tgccgcttca
     1681 ttttctaaac catcaagttc accagaatta atatgtttac cttcaccagt gtatgctgct
     1741 tcttcaacat ttccaccttc gatgacttca atgattggca aatcaaactt tttagcaaat
     1801 tcataatctc tgtcatcatg cgctggtact gccataattg ctccagtacc atatgttgat
     1861 aatacataat cagcaatcca aatttgtact ttttcaccag ataaaggatt aattgcatat
     1921 gcaccagtaa acacacctga tttatctttt gctaaatctg tacgttctaa atctgacttt
     1981 ttagaagctt ctgtttgata agcttttact ttttctttat attcatcagt tgtaattgaa
     2041 ttaactaatg catgttcagg acttaagact aagaatgatg caccatagat tgtatctggt
     2101 ctagtcgtaa atacttctac ttttccttcc gcttccgtat tatctacatc aaatgaaact
     2161 ttggcccctt cagaacgtcc aatccaattg cgctgcatat cttttaaaga ttcaggccaa
     2221 tctaaatcat ctaaatctgc taataattga tctgcatatt ctgtgatttt aagtacccat
     2281 tgtttcatcg gcttacgata aactggatgt ccaccacgtt cagagacacc atcaatcact
     2341 tcttcgttag ataaaacagt gcctaatgct ggacaccagt taactgcaac ttcatcaacg
     2401 tatgctaaac ctttgttata taactgtatg aaaatccact gtgtccattt atagtattct
     2461 ggatctgttg tattaacttc acgatcccaa tcataactga accctaattc tttaatttgt
     2521 cgtttaaaag tttggatatt tttctttgta aattcacgtg ggtcgttgcc agtgtctaaa
     2581 gcatattgct ctgctggtaa tccgaatgca tcccacccca tcggatgtaa tacattatat
     2641 ccttgcattc ttttatatct tgaaatgata tctgttgctg tatagccctc aggatgtcca
     2701 acatgtaaac cagcacctga tggatatgga aacatgtcta aagcataaaa tttcttttga
     2761 cctaagttat catttgtttt aaatgtttta ttttcgtccc aatagtcttg ccatttcttt
     2821 tcaatttgat tgtggttgta attcaacaca tttcctccta ttcaataaaa aagacacctc
     2881 taactattct atttacatag ggacgacatt tgccgcgtta ccaccctatt tcacaaaaat
     2941 tgtgcactca ttttaatagt aagagatgtc attcattcaa tttaaaatta atacgattta
     3001 catttagtcc ttaacactag ctctcagcat cactagcttt ctgtagaaaa gctccaaacg
     3061 aaattaaatt atacaaatta atattatgtt ataacgacat ttatttcaag tctataacgt
     3121 taattctatt tactcattat gcatctattt ttttaggttg cgtattattc tccttgaaaa
     3181 ccattaataa tattaatgca aatacaagta gtagcatcat accgataaac atcatgcgca
     3241 tattaaacgc atcaactaat acaccaccaa gaaatggacc aaatgctttt cctactgtag
     3301 cagctgaatt cacaaaacct tggtactgtc cttgcttacc atctggcgct aactgattgg
     3361 ctatagttgg aactgctggc catacaaaca tttctccaaa agttaaaata atcataccga
     3421 caacaaatat tgtaaagttt tcggcaaaac tcgtgacaaa gaacgacaac ataaaaatga
     3481 tgatgccgac aaacatttgc ttctttaagt ttccttttaa cagatagaga atcggtttaa
     3541 ttaatggttg tgctactaaa atcattattc cgttaattgt ccataaaaca ctatattgtg
     3601 ccattgaaat attaatagat tgtgtaaatg aagcgattgt agactcccat tgaatatatg
     3661 caacccaaca aattgcaaac attgcacaaa ttagtactaa tgaaataaat cttgctttat
     3721 tctttttacc agtaatatct aattgagttg gatatttaac tttcgcatta atttcaatat
     3781 taaattgcgt taccgcgaca agcgcaaaca caacatacat aataagattg gctaaaaaga
     3841 tatagttaaa gctaaattct gcgacaaagc cgcccattgc agcaccgaca gccacaccaa
     3901 tattttgcgc taagtatatc gcattaaacg tttgtcttcc gccatttggc cacactgctc
     3961 cagccatagc gtatatcgca ggaataatca ttccgccacc aaaccctaac attacaagcc
     4021 atacagcata ccaaggccac ccgtgaaaga aattaagtag cgttgtacta caaagacaag
     4081 tgaaagttcc aattaaaatc gtcttgtatc cacctaattt atcaaatagt gaaccaccta
     4141 ataagtttcc aataaccatg ccaaatgaat ttatcattag cactaaacca gcaacagtta
     4201 aactttttcc aagttcttgt ttcatataaa ttgtatttaa aggccacaaa aaactggaac
     4261 cagtaatatt taacgccatg ccaattacta gccaccagac tgatttaggt atattcatat
     4321 ttcatagttc ctctctcttt tttgtcaaac tataataaac actataacat taaaatatgt
     4381 taaaatctgt acttagaact ctttttttat agaaaggaaa atgacatatg ggcaatcatt
     4441 tccaatacgc ttttgaaaac aaacgttatc acacatggaa ttaccattta aaaaataaat
     4501 ttggacaaaa aatatttaaa gttgcattgg atggcgggtt tgactgtcct aaccgcgatg
     4561 gcactgtagc acatggtgga tgtacatttt gttctgctgc aggtagcgga gactttgcag
     4621 gtaatcgtgc agattcaatc gcagtacaat ttaaagaaat taaagaaaag atgcatgaga
     4681 aatggcacga aggaaaatat attgcttatt ttcaggcatt tacaaataca catgcgccag
     4741 ttgaagtatt aaaagaaaaa ttcgaacctg tacttaaaga accgggtgtt gtaggattat
     4801 ctattggtac gcgtcctgac tgtctaccag acgatgttgt cgaatattta gcagatttga
     4861 atcaacgaac atacttatgg gttgaattag gactacaaac aatccatcaa tcaacatctg
     4921 atttaatcaa tcgtgcccat gatatgaaaa cttattatga tggtgtggca aaattacgta
     4981 agcataatat caatgtatgt acacacatca ttaatggctt acctggcgaa gactatgaca
     5041 tgatgatggc tactgccaaa gaagttgcac aaatggatgt acaaggtatt aaaattcatt
     5101 tacttcattt gttaaaaggt acaccgatgg taaaacaata cgataaaggt ttattaactt
     5161 ttatgactca agaagagtac acaaacctag ttgtggacca attagaagtg attccccctg
     5221 aaatgatcgt tcaccgaatt accggtgatg gtccaataga tatcatggtc ggtccaatgt
     5281 ggagtgttaa taaatgggaa gtattaaatg gcatcgatgc tgaattagca cgtagaaatt
     5341 catatcaagg cttgcgttac aagtctaagg tgaagcaatg aaattagaac gtatactccc
     5401 tttttcaaaa acacttatta aacaacatat aacaccagaa agtattgttg tagacgcaac
     5461 ttgcggtaac ggcaatgaca ctttattttt agccgaacaa gtaccagaag gacatgttta
     5521 tggtttcgac attcaagatt tagctttgga aaatacacgt gataaagtta aggatttcaa
     5581 tcatgtttct ttaataaaag atggacatga aaatattgaa catcatataa atgatgcaca
     5641 taaaggtcat attgatgcag ccatctttaa cctaggttat ttgcctaaag gtgataaatc
     5701 tatcgtgaca aagcctgaca cgacaatcca agctattaat tcattgctat cattaatgtc
     5761 aattgaaggt attattgtac ttgttatata tcatggtcat agcgaaggac aaattgagaa
     5821 gcatgcattg cttgattact tgagtacttt agatcaaaag catgcgcaag ttttgcaata
     5881 tcaattttta aaccaacgta atcatgctcc attcatttgt gccatagaaa aaatttctta
     5941 agcatttgct gcgctatact atttataatt taggattgag tagtcaacat ttgaaaaatt
     6001 aataccaaca acaaaaagag gttttcaact taatgaaaac ctctttttat gctattaaac
     6061 ttacacagca ataattgcgt ttaaactatt ttgcattgct gttgctctac ttgcaatcgc
     6121 atcactgatg aaattcaaca actctacttt ctcttgttgt aacttttcat taaaatgaat
     6181 aataactgtt ctttcatcgt caacaggacg ctctttgtaa attcattcta attcaactaa
     6241 attattatac gttctcgtac gcttatacgg tttaacttca acaaatctgt ccatttcttt
     6301 aagcgtcata gaaccttttt gccataaagt tagtaaaatt aaaatttctt ctctagacat
     6361 tttgtattcg ctttcaatct cgctgaaaat tgagttaatg tcacccaaaa gtgtttctaa
     6421 ttgcaaaatc ccaaatacag tgtcgttatt tacttttttc atgctaaaca tctcccaatt
     6481 aataatcaca tatttagcta ccatacaagt attaacaaac atcccaacaa tcccgaaact
     6541 tgtatgtgct aacttatgca taaaactaca agtgtaaata aacttgcttt ctattcaatt
     6601 gtcaaagttg aataaaatta aataagtata aaaaccaata atcaatagag ttaatattta
     6661 gaaataagat aatagtacat aaaacaaatc actctattac atattaattt tataatttat
     6721 atacaaagaa aatatatcaa tattaccatt aaattgcaac aattctacat atatttcttc
     6781 aaaaaagata cgcattgaat ttttattaca tataactctc atgaaaattg attaaactaa
     6841 attaattagt tagccttaaa tttaacaaat taaatcaaac cgaatgaggc taaattggtt
     6901 atttttcaag atatgatttg tataaatcaa aacatttctt acaaatcaaa tgtgtataaa
     6961 atgtgatata cattctcaac cttaaaaaag ttctaaaaaa aagatgagca tctactgtct
     7021 catcttttta gaatattaaa tttctacaat ttcgtcatct tcaacaataa agcccattgt
     7081 attgacgctg ttatttaaga aagtcagaat ataacgcatt acttcatcgc gttctggctc
     7141 attgtgaacc tcgtggtaaa aaccttgcca agctttaaaa tataattcag gtgtttgata
     7201 tttttcttta aactcatcaa ttgccctagt atcaacaatt aaatccttcg ttccatacat
     7261 taatagcgtt ggcattggtt gaatgtcatg aatatgagcc atcgtatctt tcatcgtctc
     7321 attaattgta ttataccaat gatacgttgc tttttttaac attaaaccat cgttaactgt
     7381 ttcttcaaca atttctaaat tacgtgttaa atcttttggt tctacaccaa cattaaaacg
     7441 tgtgtctttt gaaattttac ctatatttga aacaagttta tctttacgat tttttccatt
     7501 cttttgaagt tctagcatag gagaaattaa catcatcccc tcgattggca attctacttt
     7561 ttcaagtaaa tttaataaaa tcaaaccgcc aagtcctacc cctaatacat aagtaggaat
     7621 tttatattca ttagctatct ttaaccagtc tagcaaactt tcgtgatacg tttgaaagtt
     7681 ttcaatttgt cctttattag ctcttgaagt ttgaccttga ccaggcaaat ctcccataat
     7741 cacatgatag ccatttcttc ttaacatcgt aataacatat gcatatcttc ccgtatgttc
     7801 taatatatta tgagcaataa caacgacgcc tttcgcatca ttttcagctt cccacttcca
     7861 cattattata ctgccccttt ttcattaatc ttcaataaca taattatagc aaattcacta
     7921 tgtagatttc tatttatagt attattgttg tccatattat tatatataaa tgaaatcaac
     7981 atcaatgata gtgtaattat acataattat ttttgattgt ttttgatgaa aacgctttct
     8041 cgaatatttt tttcatgcta aacttattgt aaacacaagg gtttggagga gtagcaatgg
     8101 cactattaaa gaattttttt atcggattat ctaataatag ttttttaaac aacgcagcaa
     8161 aaaaagtggg cccacgtttg ggcgccaata aagtcgttgc cggaaataca attccagagt
     8221 taattaatac aatcgaatac ttaaatgaca agaatatcgc tgttacagta gacaatttag
     8281 gggaatttgt cggtacagtt gaagaaagta atcatgctaa agaacaaatt ttaacaatta
     8341 tggacgcgct tcatcaacat ggcgtaaagg cacatatgtc tgttaaattg agtcagttag
     8401 gtgcagaatt cgacttagaa ttagcttacc aaaatttaag agagatttta cttaaagcaa
     8461 atacttacaa caatatgcat ataaatattg atactgaaaa atatgctagc ctgcaacaaa
     8521 ttgttcaagt tttagatcga ttaaaaggcg aatttagaaa tgttggtact gtaattcaag
     8581 catatttata cgatagccat gaattagttg ataagtacca agatttacga ttacgtttgg
     8641 ttaaaggtgc atataaagaa aacgaatcaa ttgcatttca atctaaggaa gacgtagatg
     8701 caaattacat caaaataatt gaacaacgtt tgttaaacgc acgcaatttc acttcaattg
     8761 caacacatga ccatcgcatc attaatcatg taaaacaatt tatgaaagaa aatcacattg
     8821 aaaaagatcg tatggaattc caaatgctct atggttttag atcagagtta gcagaagaaa
     8881 tcgcaaatga aggctataat ttcactattt atgtacctta tggcgatgat tggtttgcgt
     8941 attttatgag aagattagca gaacgcccac aaaacctatc tcttgctgta aaagaatttg
     9001 tgaaacctgc tggcttaaaa cgtgttggca taattgcagc tttaggagct acagttatgt
     9061 taggtttaag tacaattaaa aaattatgcc gtaaatagag caagacataa acaataattt
     9121 aggagtctgg aacaataatc aatgttctag gctcctaaat gttatattgg cagttctgaa
     9181 acatttataa atctttgatt atgaactcac aacagtgctc tatgctttta tagattttaa
     9241 taaattagcc atttcaattg cacttactgc tgcttcggca cctttattac cagctttcgt
     9301 acctgctctt tccacagctt gttcaatact ttcagtcgtt aaaataccaa atatgactgg
     9361 tacattagtt tgatcattta ctttagaaac acctttcgcg acttcattac aaacataatc
     9421 ataatgagac gtagcaccgc gaattacgca tcctaatgta attattgcat cataatttcc
     9481 tgatgaggct aattttttag ctactaaagg aatttcaaac gcacctggca caaatgctac
     9541 atcaatattg tcttcattaa catcatgtcg aatcaaagta tcttttgcac cttcaagtaa
     9601 tcttccagtg ataaaatcat taaatcgact aactacgatt gcaactttca aatcttttcc
     9661 aattaattta ccttcaaaat tcatgttaaa atcctcctat attaaatgac ccatttttat
     9721 ttttttcgtt tccatataat catgattatg taccgtttct ggtacgataa cttcaattct
     9781 ttctgcaata tcaatgccat attgttttaa tccctcaaat ttacttggat tattacttaa
     9841 taaattgata tgttcgatgt taaaatattt taaaatctgt gcagcaatat gataatctcg
     9901 caaatcttca tcaaaaccta atgctaaatt tgcagttact gtatcatatc cttgctcaat
     9961 taattcatat gcgcgtaatt tgtttaacaa tcctatgcca cgaccttctt gaggtagata
    10021 aataatcatg ccaccatgtt cattgatata cttcatagac gattcaagtt gagcaccaca
    10081 atcacaacgt tgactatgga aaatatcgcc tgtaaggcac gcagaatgta agcgtacatt
    10141 ttcatgttgt cgaattgcac cttttgtcag tacaactatc tcttcatctg tgtaagtcgc
    10201 tttaaaacca tacatatcaa atgttccgaa atctgtaggc attttcactt ttgccttaaa
    10261 ttcaatttct ggttctaatt ttttacgata ttcaattaaa tcatcaatcg taatcatctt
    10321 taattgatgt ttttctttaa acttttgtaa atcttgtcct ttcgccatcg tgccgtcatc
    10381 attcataatc tcacaaatga caccagcggg cttggcacca gtaagtttag ctaaatcaac
    10441 agccgcttct gtgtgtccat ttctagctaa tacgccttta tcttgtgcta ctaatggaaa
    10501 taaatgacca ggacgattaa aatctttagc ttcactacta ggatcaatga gctttttggc
    10561 agtcaatgta cgttcataag cactaattcc tgttgttgta tctacatgat caatacttac
    10621 tgtaaattgc gtaccaaaga tgtcggagtt atcatcaacc atttgtacca aatccaaacg
    10681 ttgtgcaata tctttagaca ctggtgcgca tattaatccc cttgcttctt tcgccataaa
    10741 attaatggta ttatcgttca tccattcagt gaccgctact aaatcacctt cattttcacg
    10801 attctcatca tctactacaa taattgtttc tccatttttt aaagccatta aagcactgtc
    10861 aatattatcg aattgcatgc tacccctcct aaaaaccaaa tgctcttaat ttatctacag
    10921 ataattggtc tttatcttta tttaaaatat tttcaacata tttaaacaaa acgtctgttt
    10981 ctaaatgtac tttatctcct aattttttgg atgataaaat cgttgaacgc ctcgtttctg
    11041 gaataagatg aatgtcaaaa ctgttatcat gcttatcaaa taccgttaga cttacaccat
    11101 ccacagtaat agacccttgc ttaactaact gattattaat atgttggcta cattgaatcg
    11161 taataatttt ggcattggct gtttcattta tttttgaaac tgttcctagt tcatctacat
    11221 gaccgaggac aaaatgtcca ccaaacctac cgttaccact catggcacgc tctaaattta
    11281 cttctgattg tcgcttaaca tctgctaaat aggttttatt ttcagtgcct ttaattactt
    11341 gaacagtaaa agatgtctga tcaaaatcaa tcactgttaa acatgcacca ttaacactga
    11401 tggaatcacc aatatgcata tctgccgtaa tcttatgtgc ttcgatttca atcgtcctga
    11461 ctgattgacg aatttgaaca cttttaacga cacctatttc ttcaacgatg ccagtaaaca
    11521 tgcatcatca cttctttcgt aaagttaatt taacattttg atttaataac tcggaatgaa
    11581 caatttcaaa ttggttcgca tctggtatct caatcacatc atttgtttga taaaattgat
    11641 aatttccaga tccgccaatt aatttcgggg cataatagag aataaattca tctatataat
    11701 tagattggag aaattctgaa gtagtggttg gacctgcctc gactagcaaa gttccaactc
    11761 ctcttttata taaattgtga agaattgttg ttaaatcgca agacttcaag taaataattt
    11821 caatatgtgt ttgattggtt gttaaatttg gattttcagt atatatccaa attggtgttg
    11881 attcatcttg ataaatttgc tgattaaaat gaatattccc agacttagac aatattactt
    11941 ttataggttt ttttccatct tgaatacgtg tagtatattg tggatcatct aattcaactg
    12001 tacgtcttcc agttaacact gcgtcgtgtc gatgtcttaa cttatagaca tcttgtttaa
    12061 cctctttgtt agtaatccat tgactttgtc cattatcatt cgcttgttta ccatctaaac
    12121 ttgcagatac tttcactgta atttgtggca gttgctttgc ttttgcttta aaaaagtctt
    12181 ggtataattg tgatgcccgt tcatcatcaa cgcattcaac ctcaataccg tgagcccgta
    12241 acgtctcatc accatgtgtg tctaacgaat tgtcttttgt tgcgtaaact actttagtta
    12301 tcttacaatc aataattttg ttaacacagg gtggtgttga accaaaatga ctacatggct
    12361 ctaacgtaat ataaatcgtc gcaccttcag cattttgttg tgccatatca agtgcttgaa
    12421 cctccgcatg cttgtcacct tttctcaagt gtgcaccaat accaacaatc ctaccttctt
    12481 taactacaac agcgccaacg ggtggattaa cacctgtttg accttgtacc atatttgcaa
    12541 gttgaatcgc ataatccata aattgactca aatgatcacc tctataaaca aaaatcctca
    12601 catcatgaat taagatgcaa ggagaaaaat ttatcgttaa ataagcctat ttgtacacat
    12661 ttttacaaat acgctacatt atctttgtcg ataattaaca ttctttctcc catccagact
    12721 ttaactgtcg gctctagaat ctcactagat cagccactaa tatgaaacat attagcaggt
    12781 cgcaggcttt atttactgcc ggttgggaat ttcaccctgc cccgaaagaa ttatatatga
    12841 aattgttata gattatttga gtacgtagta tgtcaactac atttaaaatg atactatatg
    12901 ttttctgaaa aaacaattaa tgacggtttt aatttaatat aatctgagta ctataggcat
    12961 ctcattgata tgattcttac taacagacat taaaatcaaa ccttcaattc gtctctatag
    13021 agcgttctct ttattatctt ctagttacaa attattgatt gtcactgcgc tgttgttgct
    13081 cattcgattc taaagcatca tataattgag atactttatg cgcaacttgt tctacaatca
    13141 ttttcacacc atttcgtagt ttattaacac cgtttgtcat ttgacctatc gcaatcatat
    13201 ttgttaatgt tccaaacctt ggactaataa cttgattggt ttccggaatg atttgtatgc
    13261 ctcccattgg gtgtgcttgt acaatttgtc tattttcaag atttttaatt aattgatcat
    13321 cttgatccaa ttcatttaaa tgactttttg cacctgtcgc gttaatgaca acattatata
    13381 tgtctactga ttcttggttt ttgtatgaaa aataatacaa cttgccatca tgtttcacat
    13441 cttctaaatc ttttttcaaa attaaagact tattttctat taattcaata attagttcag
    13501 cagttcttgg aggcattgga tttgaattta attgaatcat ctttgagtat ttttgattaa
    13561 attgatgttg gtcttcaata cttaagctat tccatatcca atttaaattc tctttcaaat
    13621 gttcaatcat actttggaaa atgcccattt ctgttggacg cgctaaatca tacttcaaat
    13681 ctgcaatatg atttcctgta cgtctatata ctaatttttt aaaatcaatg tcatattcag
    13741 cacattcttt taaaaataaa gaaactaaag tatcaagcgg tgcattgccg aaatgatgtt
    13801 ttttaatgtc atttaatttg tctttagtta agtacttgaa tgtcacgtct atcattgtac
    13861 ctcttacact tggtaaatga gcagaacgac tcgtcatagt aattggtaat ttcggatgat
    13921 gagcagcaac ataacggaca acatctaaac tggcaaggcc tgtaccaata atcgcaatat
    13981 cgtccagttc atttacttcg tctaacgtat tatatgttgg ataaggcgta gcgatatatc
    14041 cttttttacc ctttaagtta tatggatcat ggtaggcaaa tgtaccacat gttaaaaata
    14101 cataatcgta cgcttgccat gattgttctg aatttgtagt acatatgtaa taagttaaat
    14161 tcgtttcatc gatattagaa tttgtataaa tctcttgaac tttattataa ttagttgata
    14221 tatttggata ttttttcgtg aacatagata aataagattt catataatgt ccgaatacaa
    14281 atctcggtaa atatgcaggt tcatcaaaat taaaatcagt ttgttgttta taccacttcc
    14341 aaaattcagt ctcatcatct aaatttaaac tcatcttttt cgaaggcata ttaattagca
    14401 gctcagaact atcattttga aatggtacgc cctgtcccat atttacttta tcatcgtata
    14461 aatctatatc taattgatta aacttcgggt gcttaactaa ctctctcaat acacttacac
    14521 cagcagttcc catgcctatt attgctacac gcatatatca tccatccttt tgtaattcaa
    14581 aatattatat ttaaactatt acctataa
//
LOCUS       DBNENW010000058        14425 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNENW010000058 DBNENW010000000
VERSION     DBNENW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.058"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(141..656)
                     /locus_tag="AC1LW8_001413"
     CDS             complement(141..656)
                     /locus_tag="AC1LW8_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIP4773251.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            1036..1884
                     /locus_tag="AC1LW8_001414"
     CDS             1036..1884
                     /locus_tag="AC1LW8_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIP4773252.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            1913..2467
                     /locus_tag="AC1LW8_001415"
     CDS             1913..2467
                     /locus_tag="AC1LW8_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HIP4773253.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            2520..4232
                     /locus_tag="AC1LW8_001416"
     CDS             2520..4232
                     /locus_tag="AC1LW8_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773254.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQKINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIVALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            4229..5062
                     /locus_tag="AC1LW8_001417"
     CDS             4229..5062
                     /locus_tag="AC1LW8_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP4773255.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISVSSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELI
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     Y"
     gene            5109..5606
                     /locus_tag="AC1LW8_001418"
     CDS             5109..5606
                     /locus_tag="AC1LW8_001418"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773256.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            5724..6848
                     /locus_tag="AC1LW8_001419"
     CDS             5724..6848
                     /locus_tag="AC1LW8_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIP4773257.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            7152..7970
                     /locus_tag="AC1LW8_001420"
     CDS             7152..7970
                     /locus_tag="AC1LW8_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIP4773258.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            7988..8602
                     /gene="hisG"
                     /locus_tag="AC1LW8_001421"
     CDS             7988..8602
                     /gene="hisG"
                     /locus_tag="AC1LW8_001421"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIP4773259.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            8595..9845
                     /gene="hisD"
                     /locus_tag="AC1LW8_001422"
     CDS             8595..9845
                     /gene="hisD"
                     /locus_tag="AC1LW8_001422"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIP4773260.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            9861..10874
                     /locus_tag="AC1LW8_001423"
     CDS             9861..10874
                     /locus_tag="AC1LW8_001423"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP4773261.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLLPEQIIAGNGSDELIQKLMLIMPKGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGMAYDVEMAPHILRMRTLSKAFGIAGLRLGVLISTAGTIQRIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYGEPVMKGYVRYSIATAPQLNQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            10843..11421
                     /gene="hisB"
                     /locus_tag="AC1LW8_001424"
     CDS             10843..11421
                     /gene="hisB"
                     /locus_tag="AC1LW8_001424"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIP4773262.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            11418..11996
                     /gene="hisH"
                     /locus_tag="AC1LW8_001425"
     CDS             11418..11996
                     /gene="hisH"
                     /locus_tag="AC1LW8_001425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIP4773263.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            11989..12693
                     /gene="hisA"
                     /locus_tag="AC1LW8_001426"
     CDS             11989..12693
                     /gene="hisA"
                     /locus_tag="AC1LW8_001426"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIP4773264.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            12690..14077
                     /gene="hisF"
                     /locus_tag="AC1LW8_001427"
                     /pseudo
     CDS             12690..14077
                     /gene="hisF"
                     /locus_tag="AC1LW8_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
     gene            14237..>14425
                     /locus_tag="AC1LW8_001428"
     CDS             14237..>14425
                     /locus_tag="AC1LW8_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773265.1"
                     /translation="MDNHNTKPKIIMKSELSNSGIDVTVKDVEKYMNRYNEVMKGKNG
                     EKAKELCLSLLPINIIVVF"
BASE COUNT         5097 a         2069 c         2771 g         4488 t
ORIGIN      
        1 ttgttacttt atggtgttat tatcaattaa gttaaatatg atttttaatt tagaagttaa
       61 aaaatgccaa ctatacctag ggatgatata gttggcattt cgtatatata tattaaaaca
      121 atgaggattg tgtaatgaga ttattctgag attgagaatt cagctgatgc ttcgaattta
      181 acatctttgc ctagcattac gccaccagtt tcaagtgctt ggttaaagtt aatgccgtag
      241 ttttctctat tgattgtacc agtaacaata acacctgtta cttgagaacc atccattgga
      301 tttttactta ctccgttgaa ttcaacatcg aatgtttctt cgttagtgat gcctttaatt
      361 gttaaatcac caacaacttt gctttcagat actgattttg tcacaaatgt aattttatca
      421 aattcgtcag taccaaagaa atcaccagat tttaagtggt tatcacgtgc ttcgttttta
      481 gtgttaattg agcttggaat aattgttgca gtagctttca aagtactgaa gtcattaata
      541 tctccttcaa cagctacatc aaattgatca aatgaacctt tcactttaga aaccattaaa
      601 tgtttaattt ggaattctaa actactgtgt gcaccatcaa aagtaaagtt agtcataata
      661 taaaacctcc atatttttat ctcttagata tattttttat acttgatata catacaatac
      721 cacctaaaaa acaaagatgc aagcatttta tttcgaattc gaatattttc tgtgatgtat
      781 ttaaagaatg acctataaaa caccacacta ttgttatacc ctagtcaatt aattcaaaca
      841 taaagaagtg attacaagtc cgtgaaattt agttaatgag ataaatctga ctattattat
      901 catttttata gtcactacta ttgactttgc atcaagtgac tcgtactatt ttaatataat
      961 tctgaaaatt caaaatacag aacattaaat atgtagataa tatttttttg aagttttaaa
     1021 aaaaggaggc attttatggg aaattatttg gtattgcaat cagattttgg acttagtgat
     1081 ggcgctgtaa gtgcaatgta tggagttgca tatactgtaa gtgatgatat acgtgttgaa
     1141 aacttaacgc atgatatacc accgtatgac atttgggtag cgtcatatcg tttatatcaa
     1201 actgtaaaat actggcctaa aggcacagtt tttgtatcag tggtagaccc gggtgtaggt
     1261 agtgataggc ggagcattgc ttgcttaaca tattcaggtc attacattat tacacctgat
     1321 aatggttctt taagtcatat taaacattac gaaggtatta aaaaagttat cgaaattgat
     1381 gaagtgaaaa gccgattgcc tcattcggaa gaaagtcata cttttcatgg tagagatgtt
     1441 tatgcataca atggtgcgag attagctgca aataagatag cgtttgaacg tctcggtcag
     1501 gcaattaatg ttgatagtat tgaggcatta gaaataagag aagcgacaaa aaatgaggat
     1561 tctgttacag gaagcattga tattttagat atcagatttg ggtcattatg gacaaatatt
     1621 ccattggcat tcttaaaatc cttggaaatt gttcatggta ataatttggt ggtaacaatt
     1681 tatcatcaag ataagaaagt ctatcaaaat attattaaat ttgcgcgatc gtttgcagat
     1741 gttaatgtgg gtgaacctct tgtgtatgtt aactcgctag taaatattgg tgtagcggtc
     1801 aatcaagact cattttcaga tttatatcat attggtactg gaaatgattg gacgattcat
     1861 ttatctaaag caccatcaat ttagtgaatt ttttatgtaa ggagtaatga agatgaaaaa
     1921 acaagatatt tcagttaaaa ctgttgttgc gattggtata ggagcagcgg tatttgtgat
     1981 tttagggcgt tttgttgtaa taccaacagg ttttcctaat acgaatatag aaacatctta
     2041 tgcattttta gcattaatat ctgcaatatt tgggcctttt gctggtttaa tgactggatt
     2101 agttggacac gctattaaag atttcacgac atacggtagt gcttggtgga gttgggttat
     2161 ttgttcagga attataggct gtttatatgg atggattggc ctaaaattaa atctttcttc
     2221 aggccgattt tctaggaaat cgatgattta ttttaatatc ggtcaaatta ttgcgaatat
     2281 tatttgctgg gcacttattg caccaacatt agatattttg atttataacg aaccggctaa
     2341 caaggtttat acacaaggtg ttatctctgc agtattaaat attatttcag ttggcattat
     2401 tgggacaata ttattaaaag catatgcttc atctcaaata aaaaaaggta gtttacgtaa
     2461 agaataatca ttttgttgaa tcagatatgt aaatgaatgt agaaaggtaa tgatatatca
     2521 tgactgaacc aattatctcg ttcaaagact ttagttttca atatcatagt caagcaacac
     2581 ctacattaca gaaaataaat gttgatattt atccaggaga aaaagtatta gtagttggtg
     2641 cttcgggtag tggtaaatcg acttttgcaa attgcataaa cggattaatt ccatttaaaa
     2701 ctaaaggtaa cataactggg gaactatata taaataatca agatgcaacc gttagttgtt
     2761 tacatgatag atctaatgtt gttggtacag ttttacaaga tacagatgga cagttcatag
     2821 gcttaacagc agctgaagat atggcctttt tattagaaaa taattgtgtt gaacaagatg
     2881 atatgaagaa aaatgtaagt tattgggctg aaaaagttgg catgatagaa catttaaatc
     2941 accgaccgca agatttatct ggaggtcaaa aacaacgcgt ttcattaggt ggtatattaa
     3001 tccatcgtac gcctatttta atattggatg agccgctggc caatttagat cctgcgacag
     3061 gacatgaaac gctgagattg ttaaacaata ttcatgaaga aacaaagtca acgatgatta
     3121 ttgtcgaaca tcgattagaa gaatcattag atgatacgtt tgatagagtt cttcttttta
     3181 aagatggaaa aatcatcgct aatacaacgc ctagtgattt gctaaaatca tctaagctta
     3241 aagaagcagg catacgtgaa ccattatact gtacagcgct taaatatgca gaagtagatg
     3301 tcgaatccat tgataactta gcgaatttac gtgacgtttg tatgagtgaa catgttaaat
     3361 ttaaagtgaa aaaatggata gatgaaacat ctgcaaataa tgataataag tacaaatctg
     3421 aaccgctatt agaattaaat gaggtatgtg ttcaatatag tgactatagt aatagtgtac
     3481 ttaataatgt tcaattaaat gtttatagaa gagaaatgct tagtatagtt ggtcacaatg
     3541 gtgccggcaa atcaacactt gctaaagcaa tttgtggatt tttagatata acaggtaata
     3601 tccaattttg taatagggga tttaatcaat tgtcaattag cgaacgatct gaattcgtag
     3661 gttatgtgat gcaaaatcca aatcatatga tttccgaaaa aatgatttac gatgaagtag
     3721 cattagggtt aagagcacga ggtatgaaag aatcagatat aaagatacgg gtagagaatg
     3781 tgctcaaaat atgcggactt tatgcatttc ggaattggcc aattgtagct ttaagctatg
     3841 gtcaaaaaaa gagggtcact atagcatctg ttttagtctt aaatccggaa ataatcatat
     3901 tggatgaacc gactgctggt caagatttct atcattataa tgagataatg tcatttttaa
     3961 ttgagctaaa cagacaaggg aagacgatta ttatgattac gcatgatatg catttattgt
     4021 ctgagtatag ttcaagaaca gttgtattat caaaaggtca agtcgttgct gataccacgc
     4081 cagtattggt tttaaatgat aaaaaaatct gtgagattgc atcattgaga caaacatcgc
     4141 tatttgaaat ggccgaatat atagggatta gcgagccaca gaaattagta caattattta
     4201 ttaaccatga taggaaggtg agacgccaat gaatcaatat aatactatag gttttcaccc
     4261 gggaaatagt cgtattcatc aattaaatgc gactgttaaa cttttattct tattagttgt
     4321 ttctatttct gcaatggtga cttatgacac aagatattta attttaatta gtgtttcatc
     4381 tattttattg ttcaaatatg ctcatattga atggaaacaa gttcgttttg ttgttaaatt
     4441 cattctgttt ttcacaatat taaatattat tgccgtatac atatttgatc ctgaatatgg
     4501 tgtgaagatt tataatcagc gtacagagtt aatcaatggt attggtcgat ttacgctaac
     4561 atcacaggaa ttattctatc tttttaatct aatattaaaa tatattagta cagttccttt
     4621 agcgttaata tttttattca caacgaatcc gagtcatttt gctgcaagtt taaatcagct
     4681 aggtgtgaat tataaaatca gttacgcagt ctcactagca ttaaggtata ttccagatat
     4741 tcaagaaaca tattttaata tttcacaagc acaacaagca agaggatatg ataattcgaa
     4801 aaaggcgaaa tttactagcc gtgttaaagg tatcaaacgc attgtgttac ctttaatatt
     4861 ttctagtatc gaaagaattg acactattag tactgctatg gagttaagac aattcggaca
     4921 gtataaaagg agaacctggt acgtcaaaaa acaattaaaa aaagatgatt atgttgtttt
     4981 gtgtttgacg ttaatacttc tgatgttagt agttacatta ttctttttaa ataatagtcg
     5041 atatttcaac ccgtggtatt agcattcata taaatagtct ttaaatagaa ataggaggga
     5101 gacatttaat gataaatact gaaagattaa atttaatgat tccaagttcc tcgcatttaa
     5161 ttgaacttta taatatttgt agtcatccac aagcaaatat atacactccc aaaggtttac
     5221 ataattccaa attagacaca caacggtgga ttgaaaaatg gcgaaaccat tggcaacaat
     5281 atcaatttgg ttactttgta ttggtaaaaa aaatagattg tagtgttatt ggtatttgtg
     5341 gatatgaata tcgacaatta aagcaagaaa cagtattaaa tttattttat aaattacatc
     5401 caagttttga aggacaaggg tacgcatgtg aggctattac agcaatcaca aattttgtga
     5461 attatatcga tcaagaaaca gtaaaagtta tcaggacaaa taagtgtaac caacgttcaa
     5521 taaatttagc agaaaggctt aaattcaagc gagacaaagc tatggacgac attatcaatc
     5581 aaggagatat tgtgttttat aaataaaata ctatgacatt atctaaaaaa taaaattaaa
     5641 atcccaacag tttcttaaaa agttatgagc cacataataa aaatgttata attgtgcata
     5701 ataaaacata gggagaatgt ttaatgcata aaaaatattt attgctattt tttctgttat
     5761 tatcagttgc attgctatat agctgttcca agcaaaatga gaaagctcaa aataaatctg
     5821 ttaatccaaa tgagtatgca aagcataaat cgaaaatgga tgaacattgg gaaacatatc
     5881 acggccaagt ttatcatgtg ttttatcacc cattaattac agacccaaaa gttgcgttta
     5941 caggagatac acaccaagct aagggcaaca atgattggat gattactgta agtgaattta
     6001 aaaaatcatt gaacgaatta tataaaaata attacatcat cgttaatcca catgatgtat
     6061 acgacttatc atctcatcat gtaaaagcca aaaaaataaa attgccgaaa ggtaaaaagc
     6121 cactaatcat ctcaattgat gatatgaatt actatagtta catgagacac catggttatg
     6181 ctgaccgttt agtactcgat aaaaataaac atgttgtttc tgaaactaca gacaaaaaag
     6241 gtcatgctac ttattctgat gacaatgata tcgtgccaat tttaaataaa tttgtaaaag
     6301 agcatccaga tttttcttta aatggtgaaa aaggtgttgt tgctttaacc ggttatgagg
     6361 gcgtattagg ttatcgcacg aatgaattaa cgagtaaaga ttatacaaag aataaaaagg
     6421 cagctgagga agttgttcgc gcaatgaaac gggatggatg gtcatttgcg agtcattcat
     6481 atggtcacat taattttgaa aaaacatctt tagaaggtat taaacgtgat actaaaagat
     6541 ggaaagatga agttgaacca attgtaggta aaacagatat gtttgtattt ccacatggcg
     6601 cacaggatag acatacacaa gcgtatgatt atttggtaga tgaagcagag tttaagttta
     6661 ttgcaggtgt cggtccaaac aattttacag atatatcagc aacgaacgtt taccaagata
     6721 gggttgcgat tgatggtcta aacttatttg aatttaaata taaactcaaa ccgtttttta
     6781 atcctgaaaa tgtatatagt aaacaagata gacaattctt taaaggtaat cgggattatg
     6841 aagaatagtt aaatttaata aaaagataga aagaaaataa ctttacataa taaggcaagc
     6901 acaatatgtg caagaagtac aacttcaata taaaaatctt ggtttagcat ttttataggc
     6961 acaaatgtct tgcctttttc ttttgtaaaa atttaaaaac aatacaccga attttctgaa
     7021 aaaggttgta atgtattgct aaaaatgata agttattaat catattacac tttagtgtgg
     7081 tagagaagta aagtaaacaa cgaaaatacc aagtgatttt aaatacagta ttgaaagtaa
     7141 agggtggtcg agtgaataat tcagaacaat taattgcgtt aaaagaatcg gaaacagcat
     7201 ttttaaaata ttttaataaa gcggattatg agttagttga ttttagtgtg gttgaaaagt
     7261 tggactggaa acaattgaac catgaggatt tgcagcagat gggtgagcga aatttttggc
     7321 aacatgagca tcaaatttat gcacttcgaa atgattttac agatcaatta ttgcgttatt
     7381 actcaatgta tccaactgca gcaacaaaag tggcatacac aggtttaatc attcggaata
     7441 atgaagcggc tgtacaagtg ggtttggaaa actatgcacc atcattagcg aatgtacaac
     7501 agagcttaaa gctatttatt cagtttattc aacagcagtt gagagacaac gtgcattttg
     7561 ttgtattggg acattatcaa ttgcttgatg ccttactcga taaatcgtta caaacaccag
     7621 acatactatc aatgattgag gaacgtaatt tatcgggact ggttacatat ttgtcgactg
     7681 aacatccaat cgtgcaaatc ttgaaagaga atacgcaaca acaattaaat gtattggaac
     7741 actatattcc aaatgaccat ccagcactcg ttgaattaaa aatatgggaa cgttggttac
     7801 atacacaagg ttacaaagac atccatttag atattactgc gcagccacct agatcctatt
     7861 acacaggctt atttattcaa tgtcattttg ctgaaaatga atctcgagtt ttaacaggtg
     7921 gttattacaa aggaagcatc gaagggtttg gattaggatt aacactttaa gtaagggagt
     7981 atgcacaatg ttaagaatcg ccatagccaa aggacgtcta atggatagtt taattaacta
     8041 tttagatgta attgaatata cgacattatc agaaacatta aaaaatagag aacgccaatt
     8101 attattaagt gtagataata ttgaatgcat tttagtaaaa ggaagtgacg tgccaatcta
     8161 tgtggaacaa ggaatggccg acataggcat tgttggtagc gacatattag atgagcgcca
     8221 atataatgtt aataatttgt tgaatatgcc ttttggagca tgtcattttg cggttgcagc
     8281 gaaacctgaa acgaccaatt atcgtaaaat cgcaacgagt tatgttcata ctgctgaaac
     8341 atattttaaa tcaaaaggta ttgatgtcga attgattaaa ttgaatggtt ctgttgaatt
     8401 agcttgtgtc gtagatatgg tagacggaat tgtcgacatc gttcaaacag gtactacgct
     8461 aaaagcgaac ggactggttg aaaagcaaca tattagtgat atcaatgcaa gattaataac
     8521 taataaagca gcttatttta aaaaatcaca attaatagag caatttattc gctctttgga
     8581 ggtgtctatt gccaatgctt aatgcacaac aatttttaaa tcaattttca ttagaagcac
     8641 cattagatga gtcattgtat ccaattattc gcgatatttg tcaggaagtt aaagttcatg
     8701 gagataaggc tttgaaaatg tataatctaa cattcgatca tacgaaaaca gatcatttag
     8761 aaattagtca tgaacaaatt aaagcagcat ttgacacatt agatgaaaaa acaaaacaag
     8821 cattacaaca aagttatgaa agaattaaag catatcaaga aagtattaag cagacgaatc
     8881 aacagttaga agaatcagtg gagtgttatg aaatatacca tccactagaa agtgtgggta
     8941 tttatgtgcc tggtggcaaa gcaagttatc catcaacggt tctaatgaca gcgactttag
     9001 cacaagtagc gggtgtagaa aatattgttg ttgtgacacc acctcaacct aacggagtat
     9061 cccaagaggt attagctgca tgttatatta cgcaagttaa tcaagtgttt caagttggtg
     9121 gtgctcaaag tattgctgca ttgacttatg gaacagaaac gatacctaaa gttgataaga
     9181 ttgtaggtcc aggtaaccaa tttgttgcat atgccaaaaa atatttattt ggacaggtag
     9241 gtattgatca aattgcagga ccaacagaaa tagcactgat tattgacgac accgcagatt
     9301 tagatgccat cgtatatgat gtatttgcgc aagcagaaca tgatgaatta gcacgtacat
     9361 atgtcattgg tgaagatgcg caagtcctta aagatttaga atcacgtatt gctaaagcat
     9421 tgcctaatgt ggacagatac gacattgttt ctaaaagtat cgctaatcaa cactacctta
     9481 tccatgctag taattttgat gaagcatgcc atgtcatgaa tacaatcgcg cctgaacatg
     9541 cgtcgattca aacagtaaat cctcaaccat atattgaaaa agtgaaatat gtgggtgcat
     9601 tgtttattgg acattattcg ccagaggtca taggagatta cgttgcaggt ccaagtcatg
     9661 tattacctac aaatagaaca gctagattta ccaatgggtt atcggtcaat gatttcttaa
     9721 cacggaacac ggtcatccat ttatcaaaag atacgtttga acaaattgct gattcagcac
     9781 aacatattgc tcatgttgaa gcattataca atcaccagca gtctatttta atacgtcagt
     9841 cttaggggag tgtaattgaa atgatttata ttgataaaaa tgaaagtcca gttacgccgt
     9901 tggatgaaaa aacaatgacg tctattatta gtgcaacgcc atataattta tatcctgatg
     9961 cagcatatga acaattcaag gaagcttatg ctaagtttta cggattattg cctgaacaaa
    10021 ttattgcagg aaatggctct gatgaattga ttcaaaagtt aatgctaatc atgccaaaag
    10081 gtccggcatt aacgctaaat cctgattttt ttatgtatca agcatatgcg gcacaagtaa
    10141 atcgtgaaat tgcatttgta gatgcaggat cagatttaac gtttgatttg gaaaccattt
    10201 taacgaaaat cgatgaagta caaccatcat tttttattat gagtaatcca cataaccctt
    10261 caggcaagca atttgatacg gcatttttaa cagctattgc agataagatg aaagcattaa
    10321 acggatactt cgtcattgat gaagcatatt tagattatgg catggcatat gacgttgaaa
    10381 tggcaccaca cattttaaga atgcgcacat tatcaaaggc gtttggaata gctggcttaa
    10441 gattaggtgt cttaattagt actgctggaa cgatacagcg tattcaaaaa atagaacatc
    10501 catatccatt aaatgtattt acgctaaata ttgcgactta tatttttaga catagagaag
    10561 agacaagagt atttttaacg atgcaacgac agttagctga gcagttaaaa caaatatttg
    10621 atacacatgt tgcagataaa atgtcagtat tcccatcaaa tgccaatttt gtacttacta
    10681 aaggttcagc agcgcaacaa ttaggacaat acgtatatga acaaggattt aaacctcgct
    10741 tttatggtga gccggtaatg aagggctatg taagatactc aattgcaaca gcaccacagt
    10801 taaaccaatt agaagaaatt gttaaagaat ggagcgcaaa atatgattta tcaaaaacaa
    10861 cgaaacacag ctgaaacaca actaaatatt tcaatatcag atgaccagtc accatcgcat
    10921 attaatacag gtgtgggctt tttaaatcat atgttaacat tgtttacatt tcatagcggt
    10981 ctgtcattaa acattgaggc acaaggtgat attgacgtag atgatcacca cgtaactgaa
    11041 gatatcggca ttgtcattgg ccaattgtta cttgaaatga ttaaagataa aaagcatttc
    11101 gttcgttatg gaacgatgta cattccaatg gatgaaacat tagcacgtgt cgttgtggat
    11161 ataagtgggc gcccatacct atcattcaat gcatcattaa gtaaagaaaa agttggtacg
    11221 tttgatacgg agttagtaga agaatttttt agagcggtcg taatcaatgc aagattaaca
    11281 acgcatattg atttaattcg tggaggtaat acacaccatg aaattgaagc tatattcaaa
    11341 gcgttttccc gtgcattagg catagcgcta actgcaactg atgatcagcg tgtgccgtca
    11401 tcgaaaggtg tgattgaatg attgtcatcg ttgattatgg attagggaat attagtaatg
    11461 taaaacgcgc tattgaacat ttagggtatg aggtggttgt ctcaaatacc tcaaaaataa
    11521 tcgatcaagc agaaacaatc atattgcccg gtgtcggcca ttttaaagat gcgatgtcag
    11581 agataaagcg attaaatctc aatgcaatat tggctaagaa tactgataag aagatgattg
    11641 gtatttgttt aggcatgcaa ttaatgtatg agcatagtga tgaaggcgat gcatctggat
    11701 tagggtttat cccaggaaat atttcgcgta tccaaacaga atacccagtg ccacacttag
    11761 gctggaataa tttagtgagt aagcacccta tgttaaatca agatgtttat ttcgtacatt
    11821 cttatcaagc gccgatgtca gaaaatgtaa ttgcatatgc gcagtatggg gctgatattc
    11881 cggcaattgt tcaatttaac aattatattg gtattcaatt ccatcctgaa aaaagcggta
    11941 catatgggtt acaaattttg cgtcaggcaa tacaaggggg atttataaat gattgaatta
    12001 tggccagcga ttgatttgat tgggtcaaca agtgtgaggt taacagaggg taaatatgat
    12061 agtgaagaaa aaatgtcacg ctcggctgaa gaaagtattg cttactatag tcaatttgaa
    12121 tgtgtgaatc gtattcatat cgtcgacttg ataggtgcta aggcacagca tgcccgagag
    12181 tttgattata ttaagtcatt gaggagatta acaaccaaag atattgaagt aggtggtggc
    12241 attcgtacga agtcacaaat catggactac tttgccgcag ggattaatta ttgcatagtt
    12301 ggaacgaaag gtattcaaga tactgattgg cttaaagaga tggcacatac atttccaggt
    12361 cgcatttatt tatctgttga tgcctatgga gaagatatta aagtgaacgg atgggaagag
    12421 gacacagagt taaatttatt tagttttgtg agacggttat cggatatacc tcttggcggc
    12481 attatatata ctgatattgc taaagatggc aaaatgtccg gacctaactt tgaattaact
    12541 ggtcaattag taaaggcaac gacgattcct gtcattgctt ccggtggtat tagacatcag
    12601 caagatattc aacgattagc gtcgctaaat gttcacgctg ctattatagg aaaggctgca
    12661 catcaagcat ctttttggga ggggctaaaa tgattaaaaa acgtatcatt ccatgtttag
    12721 atgtcaaaga tggtcgtgtc gttaaaggga ttcaatttaa aggattaagg gatattggga
    12781 atcctgttga tttagcaatg tattacaatg aagcgggtgc tgatgaatta gtatttttag
    12841 acatctctaa gacagaagag ggtcatagct taatgctaga agtgattgaa cagacagcgt
    12901 cacgcttgtt tatccctctt actgtagggg gtgggattca aagtctcgat gatattaccc
    12961 aattgctaaa tcatggtgca gataaagtat cattaaattc aagtgcttta aaaaatccac
    13021 agctcattaa acaagcgagt gataaattcg gtagacaatg catctgcata gcaattgata
    13081 gctattatga tcctgaaaga aaagcacatt attgttgtac gactggtggt aaaaaaatga
    13141 caaatattaa agtatatgac tgggtacagc aagtagaaca gttaggtgca ggtgagctcc
    13201 tcgttacaag tatgggacat gatggtatga aacaaggctt tgatattgaa cacctagcaa
    13261 atattaagtc tcttgtaaat attccaatca ttgcttctgg tggtggtggc aatgcacaac
    13321 actttgtaga attatttgat cagacggatg tttctgcagg tttagctgca agtatattac
    13381 atgatcgaga aacgacggtt caatctatta aagaagtgat acggcaagga ggtatagcag
    13441 taagatgacc aattacaaaa ttgattttag caaaggttta gtgccagcaa ttttacaaga
    13501 taatcaaaca aaacaagtat tgatgttggg ttatatgaat caagaagctt ttgataaaac
    13561 gatagaagat ggtgtagtat gtttctattc gcggtcgaaa caacgtctat ggacaaaagg
    13621 tgaaacatct ggtcatacgc aacgtgttaa agatattcat gtagattgcg acaatgatac
    13681 tattttaatt gatgtcatac caaatggacc aacatgtcat acaggcagtc aaagttgttt
    13741 caacacagaa gttccatttt cagtgcaaac attagcgcag acagttcaag atagtgccca
    13801 atccaataat gaaaagtcat atacaaaata tttattaaca gaaggtatag aaaagattac
    13861 gaaaaaatat ggtgaagaag cttttgaagt cgtaattgaa gcaattaaag gtgacaaaaa
    13921 agcatttgta agtgaagtag cagatgaact ttatcattta tttgtcttga tgcatgcgct
    13981 aggcgtcgat ttttcagaaa ttgaggcaga attagcgcgt agacatcata agcgcaataa
    14041 ctttaaaggc gaacgacaaa atatcgaaca gtggtaaaac aagtatgggc tgagatatag
    14101 agaaaaggat catggcttat acacttacaa atattgtgga aaacgtgaca ttttcaagtt
    14161 taaaatacga caccaacata ttttaactat gaatgctgtg atggtactaa agttgcaaac
    14221 gcgttataga taagtagtgg ataatcacaa tacgaaacca aaaataatta tgaaaagtga
    14281 attgagcaac tcaggaatag atgtcactgt taaagatgtt gaaaagtata tgaatcgata
    14341 taatgaagtt atgaagggaa aaaatggcga aaaagctaaa gagttatgtt tgtcgttact
    14401 acctattaat atcatagttg tcttt
//
LOCUS       DBNENW010000059        14380 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNENW010000059 DBNENW010000000
VERSION     DBNENW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.059"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            50..343
                     /gene="esxA"
                     /locus_tag="AC1LW8_001429"
     CDS             50..343
                     /gene="esxA"
                     /locus_tag="AC1LW8_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HIP4773266.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            426..3455
                     /gene="esaA"
                     /locus_tag="AC1LW8_001430"
     CDS             426..3455
                     /gene="esaA"
                     /locus_tag="AC1LW8_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HIP4773267.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSRSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKTTSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3455..3913
                     /gene="essA"
                     /locus_tag="AC1LW8_001431"
     CDS             3455..3913
                     /gene="essA"
                     /locus_tag="AC1LW8_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HIP4773268.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3885..4127
                     /gene="esaB"
                     /locus_tag="AC1LW8_001432"
     CDS             3885..4127
                     /gene="esaB"
                     /locus_tag="AC1LW8_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HIP4773269.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4140..5474
                     /gene="essB"
                     /locus_tag="AC1LW8_001433"
     CDS             4140..5474
                     /gene="essB"
                     /locus_tag="AC1LW8_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HIP4773270.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5496..9935
                     /gene="essC"
                     /locus_tag="AC1LW8_001434"
     CDS             5496..9935
                     /gene="essC"
                     /locus_tag="AC1LW8_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HIP4773271.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSSVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKDYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSIFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSKSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPSDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            9965..10357
                     /gene="esaC"
                     /locus_tag="AC1LW8_001435"
     CDS             9965..10357
                     /gene="esaC"
                     /locus_tag="AC1LW8_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HIP4773272.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKTSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10373..10687
                     /gene="esxB"
                     /locus_tag="AC1LW8_001436"
     CDS             10373..10687
                     /gene="esxB"
                     /locus_tag="AC1LW8_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HIP4773273.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPIADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10687..11361
                     /locus_tag="AC1LW8_001437"
     CDS             10687..11361
                     /locus_tag="AC1LW8_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HIP4773274.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFNTDENH
                     INKAEE"
     gene            11361..11678
                     /gene="esxD"
                     /locus_tag="AC1LW8_001438"
     CDS             11361..11678
                     /gene="esxD"
                     /locus_tag="AC1LW8_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion system extracellular protein
                     EsxD"
                     /protein_id="HIP4773275.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKASHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11688..13532
                     /locus_tag="AC1LW8_001439"
     CDS             11688..13532
                     /locus_tag="AC1LW8_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIP4773276.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRSFKENGQWYKLEKL
                     WEKAIKSGKSIENVKVEVKYKGNSQRPTEFSVRYNINGEQFLKNIINK"
     gene            13543..14037
                     /locus_tag="AC1LW8_001440"
     CDS             13543..14037
                     /locus_tag="AC1LW8_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4773277.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYAMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQKLRNIFKKEGHEPWTSCEF
                     DFTNEGKLKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKQIEQYIKE
                     QEEE"
     gene            14248..>14380
                     /locus_tag="AC1LW8_001441"
     CDS             14248..>14380
                     /locus_tag="AC1LW8_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HIP4773278.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     "
BASE COUNT         5632 a         1969 c         2709 g         4070 t
ORIGIN      
        1 gtccctaaaa agttttgata aacttaaaat attcaggagg tttctagtta tggcaatgat
       61 taagatgagt ccagaggaaa tcagagcaaa atcgcaatct tacgggcaag gttcagacca
      121 aatccgtcaa attttatctg atttaacacg tgcacaaggt gaaattgcag cgaactggga
      181 aggtcaagct ttcagccgtt tcgaagagca attccaacaa cttagtccta aagtagaaaa
      241 atttgcacaa ttattagaag aaattaaaca acaattgaat agcactgctg atgccgttca
      301 agaacaagac caacaacttt ctaataattt cggtttgcaa taagcattct gaaattggca
      361 aagtcacatt ttctaatgtg gctttgctta tcatttttta agaaaacaac tgaaaggaaa
      421 taagcatgaa aaagaaaaat tggatttatg cattaattgt cactttaatt attataattg
      481 ccatagttag tatgatattt tttgttcaaa caaaatatgg agatcaatca gaaaaaggat
      541 cccgaagtgt aagtaataaa aataataaaa tacatatcgc aattgttaac gaggatcaac
      601 caacgacata taacggtaaa aaagttgagc tgggtcaagc atttattaaa aggttagcaa
      661 atgagaaaaa ctataaattt gaaacagtaa cacgaaacgt tgctgagtct ggtttgaaaa
      721 atggtggata ccaagtcatg attgttatcc cagaaaactt ttcaaaattg gcaatgcaat
      781 tagacgctaa aacaccatcg aaaatatcgc tacagtataa aacagctgta ggacaaaaag
      841 aagaagtagc taaaaacaca gaaaaagttg taagtaatgt acttaacgac tttaacaaaa
      901 acttagtcga aatttattta acaagtatca ttgataattt acataatgca caaaaaaatg
      961 ttggcgctat tatgacgcgt gaacatggtg tgaatagtaa attctcgaat tacttattaa
     1021 atccaattaa cgacttcccg gaattattta cagatacgct tgtaaattca atttctgcaa
     1081 acaaagatat tacaaaatgg ttccaaacat acaataaatc attattgagt gcgaattcag
     1141 atacgttcag agtgaacaca gattataatg tttcgacttt aattgaaaaa caaaattcat
     1201 tatttgacga gcacaataca gcgatggata aaatgttaca agattataaa tcgcaaaaag
     1261 atagcgtgga acttgataac tatatcaatg cattaaaaca gatggacagc caaattgatc
     1321 aacaatcaag tatgcaagat acaggtaaag aagaatataa acaaactgtt aaagaaaact
     1381 tagataaatt aagagaaatc attcaatcac aagagtcacc attttcaaaa ggtatgattg
     1441 aagactatcg taagcaatta acagaatcgc tgcaagatga gcttgcaaat aacaaagact
     1501 tacaagatgc gctaaatagc attaaaatga acaatgctca attcgctgaa aacttagaga
     1561 aacaacttca tgatgatatt gtcaaagaac ctgatacaga tacaacattt atctataaca
     1621 tgtctaaaca agactttata gctgcaggtt taaatgagga tgaagctaat aaatacgaag
     1681 caattgtcaa agaagcaaaa cgttataaaa acgaatataa tttgaaaaaa ccgttagcag
     1741 aacacattaa tttaacagat tacgataacc aagttgcgca agacacaagt agtttgatta
     1801 atgatggtgt gaaagtgcaa cgtactgaaa cgattaaaag taatgatatt aatcaattaa
     1861 ctgttgcaac agatcctcat tttaattttg aaggcgacat taaaattaat ggtaaaaaat
     1921 atgacattaa ggatcaaagt gttcaactcg atacatctaa caaggaatat aaagttgaag
     1981 tcaatggcgt tgctaaattg aaaaaggatg ctgagaaaga tttcttaaaa gataaaacaa
     2041 tgcatttaca attgttattt ggacaagcaa atcgtcaaga tgaaccaaat gataagaaaa
     2101 caacgagtgt tgtggatgta acattgaatc ataaccttga tggtcgctta tcgaaagatg
     2161 cattaagcca gcaattgagt gcattatcta ggtttgatgc gcattataaa atgtacacag
     2221 atacaaaagg cagagaagat aaaccattcg acaacaaacg tttaattgat atgatggttg
     2281 accaagttat caatgacatg gaaagtttca aagacgataa agtagctgtg ttacatcaaa
     2341 ttgattcaat ggaagaaaac tcagacaaac tgattgatga cattttaaat aacaaaaaga
     2401 atacaacaaa aaataaagaa gatatttcta agctgattga tcagttagaa aacgttaaaa
     2461 agacttttgc tgaagagcca caagaaccaa aaattgataa aggcaaaaat gatgaattta
     2521 atacgatgtc ttcaaattta gataaagaaa ttagtagaat ttctgagaaa agtacgcaat
     2581 tgctatcaga tacacaagaa tcaaaaacaa ttgcagattc agttagtgga caattaaatc
     2641 aattagataa taatgtgaat aaactacatg cgacaggtcg agcattaggc gtaagagcga
     2701 atgatttgaa ccgtcaaatg gctaaaaacg ataaagataa tgagttattc gctaaagagt
     2761 ttaaaaaagt attacaaaat tctaaagatg gcgacagaca aaaccaagca ttaaaagcat
     2821 ttatgagtaa tccggttcaa aagaaaaact tagaaaatgt tttagctaat aatggtaata
     2881 cagacgtgat ttcaccgaca ttattcgtat tattgatgta tttactatca atgattacag
     2941 catatatttt ctatagttat gaacgtgcca aaggacaaat gaatttcatt aaagatgatt
     3001 atagtagtaa aaaccatctt tggaataatg tcattacgtc aggtgttatt ggtacaactg
     3061 gtttggtaga aggattaatt gtcggtttaa ttgcaatgaa taagttccat gtattagctg
     3121 gctatagagc gaaattcatc ttaatggtga ttttaactat gatggtcttc gtacttatta
     3181 atacgtattt actaagacag gtaaaatcta tcggtatgtt cttaatgatt gctgcattgg
     3241 gtctatactt tgtagctatg aataatttga aagcggctgg acaaggtgtg actaataaaa
     3301 tttcaccatt gtcttatatc gataacatgt tcttcaatta tttaaatgca gagcatccta
     3361 taggcttggc gctagtaata ttaacagtac ttgtgattat tggctttgta ctgaacatgt
     3421 ttataaaaca ctttaagaaa gagagattaa tctaatgttg atgaatagcg tgattgcttt
     3481 aactttttta acagcatcta gcaataatgg cggacttaat attgacgtgc aacaagaaga
     3541 ggaaaagcga atcaataatg atttaaatca atatgataca acgctattta ataaagacag
     3601 caaagcggtt aatgatgcga ttgctaagca gaaaaaagaa cgacaacaac aaataaaaaa
     3661 tgatatgttt caaaatcaag cgagtcactc gactcgcttg aatgaaacta aaaaagtgtt
     3721 attttccaaa tctaacttag aaaagacttc ggagagtgat aaaagcccct atattcaaaa
     3781 caagcaggag aaaaaaatat tcccgtacat tttgatgtct gtaggggctt ttttgacttt
     3841 aggatttgtc attttttcaa ttcataaagg gagacgaacg aaaaatgaat cagcacgtaa
     3901 aagtaacatt tgattttact aattataatt acggcacata tgacttagca gtaccagcat
     3961 atttaccgat aaaaaactta atagctttag tattggatag tttggacatt tcaatatttg
     4021 atgtcaatac acaaattaaa gtgatgacga aaggtcaatt acttgttgaa aatgatcgac
     4081 tcattgatta tcaaatcgct gatggagata ttttgaagtt actataggag gaaaaataga
     4141 tggttaaaaa tcataaccct aaaaatgaaa tgcaagatat gttaacgcct ttagatgctg
     4201 aagaagcagc taaaacaaaa ttacgcttag atatgagaga gattcctaag tcttcaatta
     4261 aaccagaaca ttttcattta atgtacttat tagaacaaca ttctccatat tttatagatg
     4321 ctgaattaac tgaactacgt gacagtttcc aaatacatta tgacattaat gacaatcata
     4381 caccttttga taatattaaa tcatttacta aaaatgaaaa attacgttac ttactcaata
     4441 tcaaaaattt agaagaagta aatcgtacac gctacacatt tgtgttggca ccagatgaat
     4501 tatttttcac aagagatgga ttacccattg ctaaaacaag agggttacaa aatgttgttg
     4561 atccattacc tgtgtcagaa gctgaatttt taacaagata taaagcactg gttatctgtg
     4621 cattcaatga gaaacaatca tttgatgctt tagttgaagg aaacttagaa ctacataaag
     4681 gaacgccatt tgaaactaaa gttattgaag cggcaacgtt agatttacta acggcatttt
     4741 tagatgaaca gtatcagaaa caagaacaag attatagtca aaattatgca tatgtacgca
     4801 aagtaggaca taccgttttc aaatgggttg ctatcggtat gacaacgtta agtgttttat
     4861 taattgcatt cttagccttt ttatattttt cagtaatgaa gcataatgag cgcattgaaa
     4921 aaggatacca agcatttgta aaggatgatt atacgcaagt actaaatacg tatgatgatt
     4981 tagatggtaa aaaattagat aaagaggcac tttacattta tgccaaaagt tatatccaaa
     5041 caaataaaca aggtttagaa aaagataaga aagaaaattt acttaataat gtgacaccaa
     5101 attcaaacaa agactactta ttatattgga tggaattagg acaaggacat cttgatgaag
     5161 cgattaatat tgccacttat ttagatgata acgatattac aaagttagcg ttgattaata
     5221 aattaaatga gattaaaaat aacggagatt tatcgaatga taaacgttct gaagaaacga
     5281 aaaagtataa cgataaattg caagatattt tagacaaaga aaaacaagtt aaagatgaaa
     5341 aagcgaaatc tgaagaagag aaagcaaaag cgaaagatga gaaattaaag caacaagaag
     5401 agaacgaaaa gaaacaaaaa gaacaagcac aaaaagataa agaaaaacgc caagaagctg
     5461 aaagaaaaaa atagtatagg actgaggcaa agacaatgca taaattgatt ataaaatata
     5521 acaaacaatt gaagatgctc aatttgcgag atggtaagac atatactatt agcgaagacg
     5581 agcgtgcaga tattacgttg aaatcgttag gcgaagtcat tcatttagaa caaaataatc
     5641 aaggtacttg gcaagcgaat catacttcta ttaataaggt gcttgttaga aaaggtgacc
     5701 ttgatgacat tacattacag ctttatacag aagctgatta tgcatcattt gcgtatcctt
     5761 caattcaaga tacgatgaca attggaccaa atgcgtatga tgatatggtt attcaaagct
     5821 tgatgaatgc catcattatt aaagattttc aatcaataca agaatcacaa tacgtacgca
     5881 ttgtgcacga taaaaataca gatgtgtata ttaactatga actacaagag caactaacga
     5941 acaaagctta cattggtgat catatttatg ttgaagggat atggctcgaa gtacaagctg
     6001 atggtttaaa tgtattgagt cagaatacag tggcatcgtc attaattcgc ttaacacaag
     6061 agatgccaca tgcacaggca gatgattaca atacgtacca tcgttcgcca aggattattc
     6121 accgtgaacc gacggatgat attaagattg aaagaccgcc acagccaata cagaagaaca
     6181 atacagtgat atggcgttcc attataccgc cattagtaat gattgcttta actgttgtca
     6241 tctttttagt gagaccaatt ggtatttata ttttaatgat gattggtatg agttcagtaa
     6301 cgatagtatt tggtattaca acgtatttct ctgaaaagaa aaagtataac aaagatgttg
     6361 aaaaacgaga gaaagattac aaagattatt tggataataa atctaaagaa attaataaag
     6421 cgattaaagc acaacgtttt agtttgaatt accattatcc aacggttgct gaaattaaag
     6481 atatcgttga aacgaaagca ccaagaatat atgaaaaaac atcgcatcat cacgatttct
     6541 tacattataa gttaggtatt gcgaatgtag aaaagtcatt caaattagat taccaagaag
     6601 aagaatttaa ccaacgtcgt gatgaattat tcgacgatgc taaagaattg tatgaatttt
     6661 acacagatgt agaacaagca ccattaatca atgatttaaa tcatggaccg attgcatata
     6721 ttggtgcacg acatctcatt ttagaagaat tggaaaaaat gttaatccaa ttgtcaatat
     6781 tccatagtta tcatgattta gagtttctat ttgtgacacg tgaagatgaa gttgaaacat
     6841 tgaaatgggc acgttggttg ccacatatga cattgagagg tcaaaacatt agaggatttg
     6901 tttacaatca acgaacacgt gaccaaattt taacgtcaat ttacagcatg attaaagaac
     6961 gtatccaagc tgtgcgtgaa cgcagtaaaa gtaatgagca aattattttt acaccgcaat
     7021 tagtgtttgt cattacagat atgtcattaa ttattgatca tgtcatttta gaatatgtaa
     7081 accaagattt atcagaatat ggtatttcat taatctttgt tgaagatgtg attgaaagtt
     7141 tgccagagca tgtagatacc attattgata tcaagtctcg tactgaagga gaactgatta
     7201 cgaaagaaaa agaattagtt caattgaaat ttacacctga aaatattgat aacgtcgata
     7261 aagaatatat cgcgcgacgt ttggcgaatt tgatacacgt cgaacatttg aaaaatgcaa
     7321 ttcctgatag tattacattt ttagagatgt ataacgtgaa agaagtagat cagcttgatg
     7381 tggttaatcg atggagacaa aacgaaacat acaaaacgat ggcagtacct ttaggtgtaa
     7441 gaggtaaaga tgacatttta tcattgaact tacatgaaaa agcacacgga ccacatggtt
     7501 tagttgctgg taccactggg tcagggaaat ctgagattat ccaatcatac attttatctt
     7561 tagctattaa ttttcaccca catgaagttg cgttcctatt gattgactat aaaggtgggg
     7621 gtatggcgaa cttatttaaa gatttagtcc atttagttgg tacgattaca aacttagatg
     7681 gtgatgaagc gatgcgtgcc ttaacatcaa tcaaagccga attgagaaaa cgtcaacgtt
     7741 tattcggaga gcatgatgtt aaccatatta atcaatacca taagttattt aaagaaggta
     7801 ttgcgacaga accaatgcca catttattca ttatttccga tgagtttgcc gaattaaaat
     7861 cagaacaacc tgattttatg aaagaacttg tatcaacggc acgtattgga cgttcgttag
     7921 gtattcattt aatacttgcg acacaaaaac catcgggtgt tgttgatgac caaatttggt
     7981 ctaactctaa atttaaattg gcattaaaag tacaagatag acaagacagt aatgaaattt
     8041 taaaaacacc agatgcagca gacattacat taccaggtcg tgcgtattta caagttggta
     8101 ataatgaaat ttatgaattg ttccaatctg catggagtgg agcaacatat gacatcgaag
     8161 gcgataaatt agaagttgaa gataagacga tttacatgat taatgattat ggtcaacttc
     8221 aagcaatcaa caaagacttg agtggacttg aagatgaaga aacgaaagaa aatcaaactg
     8281 agttagaagc ggttatcgat catatagaat ctattacaac acgattagaa attgaagaag
     8341 ttaagcgtcc atggttacca ccattaccag aaaatgtata tcaagaagat ttagtagaaa
     8401 cagatttcag aaaattatgg tcagatgatg caaaagaagt ggaattaaca ttaggactta
     8461 aagacgtacc agaagaacaa tatcaagggc cgatggtatt acaattgaaa aaagctggtc
     8521 acatcgcgtt aatcggaagt ccaggatatg gtagaacaac gttcttacac aacattattt
     8581 tcgatgttgc aagacaccat cgtcctgatc aagcacacat gtacttgttc gatttcggta
     8641 ccaatggttt gatgccagtc acagacatac cacatgtcgc tgattacttt acagtagatc
     8701 aagaagacaa gattgcgaag gcgatacgta tatttaatga tgaaatcgat cgccgtaaga
     8761 agattttaag tcagtatcgt gttactagta tttctgaata tcgaaaatta actggtgaaa
     8821 caattccgca tgtctttatt cttattgata actttgacgc agtaaaagat tcaccttttc
     8881 aagaagtttt tgaaaatatg atgattaaaa tgacacgtga agggctagca ttagacatgc
     8941 aagtaacctt aactgcctca agagctaacg ctatgaaaac accaatgtac attaatatga
     9001 aaacgcgtat cgccatgttt ttatatgata aatcagaagt gtcgaacgta gtaggacagc
     9061 aaaaatttgc ggttaaagat gtagtgggtc gagcattgtt aagtagcgat gacaacgtat
     9121 cattccatat tggccaacca tttaaacatg atgagaccaa atcatataat gatcaaatta
     9181 atgatgaagt atcggcgatg acagaatttt ataaaggtga aacaccaagt gatattccta
     9241 tgatgccaga tgaaattaaa tatgaagatt acagagaatc attaagctta ccggatatag
     9301 ttgcaaatgg tgctttacca attggattag attatgaagg tgttacacta caaaaaatta
     9361 aattaactga accagcaatg atttcatcag aaaatccgag agaaattgcg catattgctg
     9421 aaattatgat gaaagaaatt gacatattaa atgaaaaata tgcgatttgt atcgcagatt
     9481 caagtggaga gtttaaagct tatagacatc aagtggctaa ctttgccgaa gaaagagaag
     9541 acattaaagc gattcatcaa ctaatgattg aagacttaaa acaaagagaa atggacggtc
     9601 cgtttgaaaa agattcactt tacattatca atgattttaa aacatttatt gattgcacgt
     9661 atattccgga agatgatgtt aaaaaactta ttacaaaagg accagaactt ggcttgaaca
     9721 ttttatttgt cggcattcat aaagaattaa tagatgctta tgataaacag attgatgttg
     9781 cacgtaaaat gattaaccaa tttagtatag gtattcgtat ttcagaccaa caattcttta
     9841 aatttagatt tattcaacga gaacctgtta ttaaagaaaa tgaagcatat atggtcgcaa
     9901 accaagctta tcaaaagatt agatggttta aatagcaatg aattaaatag gagggaggta
     9961 tgttatgaat tttaatgata ttgaaacaat ggttaagtcg aaatttaaag atattaaaaa
    10021 gcatgctgaa gagattgcgc atgaaattga agttcgttct ggatatttaa gaaaagctga
    10081 acaatataag cgattagaat ttaatttgag ttttgcacta gatgatattg aaagcacagc
    10141 aaaggacgta caaactgcaa aaactagtgc taataaggac agtgtaactg ttaaaggaaa
    10201 agcgcccaat acgttatata tcgaaaaaag aaatttgatg aaacaaaagc ttgaaatgtt
    10261 gggtgaagat atcgataaaa ataaagaatc cctccaaaaa gctaaggaaa ttgctggaga
    10321 aaaggcaagt gaatatttta ataaagcaat gaattaatat tgaggtgaag atatgggtgg
    10381 atataaaggt attaaagcag atggtggcaa ggttgatcaa gcaaaacaat tagcggcaaa
    10441 aacagctaaa gatattgaag catgtcaaaa gcaaacgcaa cagctcgctg agtatatcga
    10501 aggtagtgat tgggaaggac agttcgccaa taaggtgaaa gatgtgttac tcattatggc
    10561 aaagtttcaa gaagaattag tacaaccgat tgctgaccat caaaaagcaa ttgataactt
    10621 aagtcaaaat ctagcgaaat acgatacatt atcaattaag caagggcttg atagggtgaa
    10681 cccatgatga aagatgttaa gcgaatagat tatttttctt acgaagaatt aacaatttta
    10741 ggtggtagta aattacctct cgtaaatttt gaattgtttg atccatcaaa ttttgaagaa
    10801 gctaaagcgg ctttaattga aaaggaatta gtaacagaga atgacaagtt aactgatgca
    10861 ggttttaaag tggctacatt agtcagagag tatattagcg caattgtaaa tattcgaatt
    10921 aatgatatgt attttgcacc atttagctat gaaaaagatg aatatatttt gttaagccgg
    10981 tttaaaaata atggatttca aatacgaatt ataaataaag acattgcatg gtggtcgatt
    11041 gtacaatcat atcctttatt aatgagacaa gaaaagtcca atgattggga ctttaaacaa
    11101 attgacgatg aaacattgga gaacttaaat aatgaaagta tcgatacgat tgggcgtgtt
    11161 ttagaaattg aaatatacaa tcatcaaggt gaccctcaac aaagtttata taacatttat
    11221 gaacaaaatg atttgctact cattcgatac ccattaaaag ataaagtact gaatgttcat
    11281 attggcgtca ttaatacatt tatacgagaa ttatttggat tcaatactga tgaaaatcat
    11341 attaataaag cagaggagta atgacgttga gtggaaaaat tagtgttaaa gctgaaacga
    11401 ttgcacatgt tgtaaaagaa ttggaaagca ttagtcaaaa gtatgatgaa atagctcaaa
    11461 actttggaaa aattgcgcaa ttaaattact acagtagtga aaaagcttca cattctatgg
    11521 aaaacggcta tagtagtgct gcaacagtca ttagtggtct caaaggtcca ttgagtacac
    11581 tcggtggtgg tgtcatgaat tcagcacaaa agttctttga agcagatgaa cattggggta
    11641 cggaatttgc caagctttac tataatattg agggataggt gcatgacatg acaaaagata
    11701 ttgaatattt aacagctgat tatgacaatg aaaagtcatc tatccaaagt gtaatagatg
    11761 caatagaggg gcaagacttc ttagatgtag atacaacaat ggatgatgcg gtaagcgatg
    11821 tcagttcttt agacgaagat ggcgcaatat cattaacaag tagtgtagta ggtccacaag
    11881 gatctaaatt aatgggctat tatcaaaatg agttatatga ttatgcatct caattagatt
    11941 cgaaaatgaa agaaattatt gacacgccat ttatagaaga tatagataaa gcattcaaag
    12001 gtataacgaa tgttaaattg gaaaatatat taattaaaaa tggcggtggt catggtagag
    12061 atacctatgg ggcttctggg aaaattgcaa agggagatgc caagaaaagt gacagcgatg
    12121 tttatagcat cgatgaaata ttaaaatcgg atcaagaatt tgtaaaagta attgatcagc
    12181 attacaaaga aatgaaaaaa gaagataaga aattatctaa gagtgatttt gaaaaaatga
    12241 tgactcaggg cgcttcttgt gattacatga cagtagctga agcggaagag ctagaggagc
    12301 aaaagaaaaa agaagaagct atagagattg cagcactagc tggtatggta gttttatctt
    12361 gtattaatcc tgttgctgga gcagtagcta ttggtgctta ttccgcctat tcagcagcaa
    12421 atgcagccac aggaaaaaat attgtaactg gaagaaagct atctaaagaa gaacgaatca
    12481 tggaaggact ttcgcttatt ccattgccag gtatgggctt cctcaaaggt gctgggaaaa
    12541 gtttaatgaa attaggcttc aaaggcggag aaaaatttgc agttaaaaca ggattgcaaa
    12601 agacaatgca acaagcggtt agtcgtattt cacctaaaat gggaatgatg aaaaacagtg
    12661 tgttgaatca atctcgtaac tttgctcaaa atactcatgt tggacaaatg ctgagtaaca
    12721 tgcgtggtca agcaactcat actgttcaac aaagtagaaa ttggattgga caacaagcgc
    12781 aaaatgtcaa acgaatagtg aataatggac ttgataaaga aatagcacat ccatttaaac
    12841 aacaacttgc accagcggga atgggtggta taaaatttgc tgaaacaact actttgagaa
    12901 acatgggtca aaacatgaaa cgtgctgtta caccacaaaa tcacgtgaca catggtccga
    12961 aagatagtat ggtgagaagt gaaggtaaac atagtgtaag tagccatgaa ataaattcat
    13021 caaaatatgt tgaatcacca aactacacca aggttgaatt cggagaacac tatgcaagac
    13081 ttagacctaa gaaactaaaa gcaaatatag aatatacaac accaactggt cacatatatc
    13141 ggaccgatca taaagggcgc ataaaagaag tttatgtaga caatctttct ctaaaagatg
    13201 gcggtcgtaa taaccatgca caaagaactg tggggggaga ggatagatta ccagacgatg
    13261 atggaggtca tttaatcgct agaatgtttg gtgggtcaaa agacatagat aaccttgtgg
    13321 cacaaagtaa atttatcaac cgttcattta aggaaaatgg acaatggtat aagttggaaa
    13381 aactatggga aaaagcaatc aagtctggga agtcaataga aaatgttaaa gttgaagtta
    13441 aatataaagg taatagtcaa aggccaaccg aattttcagt tagatataat attaatggtg
    13501 agcagttttt aaaaaatata attaataaat aggaggatta acatgacttt cgaagaaaaa
    13561 ctaagtcaaa tgtataacga gattgcgaat aagattagca gtatgatacc ggtagaatgg
    13621 gaaaaggtat atgcaatggc atatatagat gatgaaggag gcgaagtgtt ctactattac
    13681 acagaacctg gaagtaatga attatactat tatactagtg tattaaataa atatgatata
    13741 tcggaatcag aatttatgga ttcagcgtat gagttgtata aacaatttca aaagttaaga
    13801 aatatattta aaaaagaagg acatgaacca tggacatcat gcgaatttga ttttacaaat
    13861 gaaggtaaat taaaagtttc atttgattat attgattgga taaatacaga gtttgatcaa
    13921 ttgggccgtc aaaattatta tatgtacaaa aaatttgggg ttataccaga aatggaatat
    13981 gaaatggagg aggttaaaca aatcgagcaa tatattaaag agcaagaaga agaatagaca
    14041 atcttaattt gagactaaac aataaagctt tgtttagtct tttttggcgg ttaagtaaaa
    14101 agcaatagag accgtaaagt tgatgctcat caaataataa tataaagata attttaggtt
    14161 tttaaacttt taatcgaaaa gaatctaagt atatatagct ttaaatacgt aatatgctat
    14221 ttaaaagata atgtatagga gatagatatg actactaaag aaaatattga tactcttcga
    14281 aagccaggtg cacaagcctt aagtttaata tcattgtttt tgatactttt ttcatgtcta
    14341 actttctttt ttggtttaga ttatgaaagg tttccaaact
//
LOCUS       DBNENW010000060        14218 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.060, whole genome shotgun sequence.
ACCESSION   DBNENW010000060 DBNENW010000000
VERSION     DBNENW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.060"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..422
                     /locus_tag="AC1LW8_001442"
     CDS             <1..422
                     /locus_tag="AC1LW8_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIP4773279.1"
                     /translation="VIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSLT
                     LLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVEK
                     GFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            796..2760
                     /locus_tag="AC1LW8_001443"
     CDS             796..2760
                     /locus_tag="AC1LW8_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HIP4773280.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNADEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            3102..4181
                     /locus_tag="AC1LW8_001444"
     CDS             3102..4181
                     /locus_tag="AC1LW8_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvI family membrane protein"
                     /protein_id="HIP4773281.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(4286..4879)
                     /gene="mhqD"
                     /locus_tag="AC1LW8_001445"
     CDS             complement(4286..4879)
                     /gene="mhqD"
                     /locus_tag="AC1LW8_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylhydroquinone degradation carboxylesterase
                     MhqD"
                     /protein_id="HIP4773282.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(4910..5869)
                     /gene="mhqE"
                     /locus_tag="AC1LW8_001446"
     CDS             complement(4910..5869)
                     /gene="mhqE"
                     /locus_tag="AC1LW8_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase MhqE"
                     /protein_id="HIP4773283.1"
                     /translation="MANNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(5946..6380)
                     /gene="mhqR"
                     /locus_tag="AC1LW8_001447"
     CDS             complement(5946..6380)
                     /gene="mhqR"
                     /locus_tag="AC1LW8_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator MhqR"
                     /protein_id="HIP4773284.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            6606..6890
                     /locus_tag="AC1LW8_001448"
     CDS             6606..6890
                     /locus_tag="AC1LW8_001448"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773285.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            7064..7870
                     /locus_tag="AC1LW8_001449"
     CDS             7064..7870
                     /locus_tag="AC1LW8_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4773286.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIALLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIASNTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7991..8662)
                     /locus_tag="AC1LW8_001450"
     CDS             complement(7991..8662)
                     /locus_tag="AC1LW8_001450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIP4773287.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            8932..9924
                     /locus_tag="AC1LW8_001451"
     CDS             8932..9924
                     /locus_tag="AC1LW8_001451"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HIP4773288.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLEDDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTSTCETRLN"
     gene            complement(10171..10971)
                     /locus_tag="AC1LW8_001452"
     CDS             complement(10171..10971)
                     /locus_tag="AC1LW8_001452"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP4773289.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKSRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFKIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            11292..11900
                     /locus_tag="AC1LW8_001453"
     CDS             11292..11900
                     /locus_tag="AC1LW8_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4773290.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKE"
     gene            11933..12664
                     /locus_tag="AC1LW8_001454"
     CDS             11933..12664
                     /locus_tag="AC1LW8_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HIP4773291.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNTLFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(12826..13446)
                     /gene="srtA"
                     /locus_tag="AC1LW8_001455"
     CDS             complement(12826..13446)
                     /gene="srtA"
                     /locus_tag="AC1LW8_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HIP4773292.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            13640..14131
                     /locus_tag="AC1LW8_001456"
     CDS             13640..14131
                     /locus_tag="AC1LW8_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP4773293.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
BASE COUNT         4595 a         2522 c         2169 g         4932 t
ORIGIN      
        1 ttgttattgg gtctttattc tttaatgatt taaatatatt tgaaagtagc ggtaatcaat
       61 tatctttatt gaatcaatta ttattagtta gtttaggcac atttgttggc gtttggttag
      121 ttttaagctt aacgttgcta ttatcatctg caacaaattc aacgggagta gccattgctg
      181 taggtattgt tttttatttt gcaagttcta ttttagcagt tattcaaacg gcacttttag
      241 aaaaaataga ctggctaaag tggaatccta ttaatatgat gaatattatg cttcaaacag
      301 ttgaaaaagg ctttagtaag tcgacgaaat tagaacttca tgaattgttt attggtaata
      361 ttgcttatat ttctattttc ttaatacttg tagtatttat tttcaagaag aaaaatattt
      421 agtaacttca agttattaaa tgtctaaata cacacatatt ccatcgtaat tcaaaatcat
      481 tttcaaatcc cttcacccaa ataatggtgc ggggattttt ttatccaaat tttggcaatt
      541 ccacatttta catatcgtaa ttttttagga aactagtgaa tataacaaat tcctcctctc
      601 atttttaaaa taaatatatc actttcccac ttttacttaa ctaaactgca acgactccta
      661 ataccaaaat cctgccctct atttttatca atccaagcat acttattgaa aaatgttaac
      721 gttttcttga taatcattgt aagcgcattt attttataaa ctaacgtttg aaatatacta
      781 caggagtgac acgtaatgac tcaaattact gaaaaagaat taaaaaagaa gtatttagat
      841 ttactatccc aaaattttga tactccagaa aaacttgcaa ctgaaattat caatttagaa
      901 tcaattttag aattacctaa aggtacggaa catttcgtca gcgatttaca tggtgaatac
      961 gaagctttcc aacacgtatt acgcaacggt tctgggaatg tgcgagcgaa aatcaatgat
     1021 attttcaaag agagactttc aactaaggag cttaatgatt taactgctct tgtctactat
     1081 ccagaagaca aattaaaatt gattaaaagt gatttccaaa gttgcggtca acttaatgtc
     1141 tggtatatca caacaatcga acatttaatt gagttgatta aatattgttc ttcaaaatat
     1201 acgcgttcaa aactgcgtaa agcattgcca aaacaatacg tttatattat tgaggaacta
     1261 ctgtataaaa gtaatgaata tcaaaataaa aaatcatatt acgaaacact tgttaaccaa
     1321 gtaattgaac ttaaacaggc agatgattta attattggac tcgcttattc cgtacaacgc
     1381 ttagtcgtcg atcatttaca cgttgtcggt gatatttatg atcgtggacc acaaccagat
     1441 aaaattatgg atacactgat taattatcat tccctagata ttcaatgggg taatcatgat
     1501 gtgctttggg ttggagccta tgctgggtca aaagtatgct tagcaaactt acttcgaatt
     1561 tgtgcacgct atgataattt agatattatc gaagacgctt atggcattaa tttaagacca
     1621 ctgcttactt tagctgaaaa atactatgac gcagataatc ctgcttttaa gcctaaaaaa
     1681 agacctgaca aacacgaacg tttaactcaa cgtgaagaaa gtcaaattac taaaattcat
     1741 caagctattg cgatgattca attcaagtta gaaataccaa ttattaaacg tcgtccaaat
     1801 ttcgaaatgg aagaacgtct tgtgcttgaa aaggttaatt atgatacaaa tgaaattaca
     1861 gtttatggta atacataccc attgaaagac acatgtttcc aaactgtcaa tcgtgataat
     1921 ccagcagaat tactacctga agaagaagaa gtcatgaata aactattatt gtcattccaa
     1981 caatctgaaa aattacgtcg tcatatgtct ttcttgatgc gtaaaggctc tctttactta
     2041 ccatataatg gcaatttact cattcatggt tgtattccag ttgatgaaaa tggtgagatg
     2101 gaatcatttg aaattgatgg tcatacttac agcggccaag aattattaga tgtatttgag
     2161 tatcatgtcc gtaaatcatt tgatgaaaaa gaaaatactg atgacttatc gacggattta
     2221 gtttggtatt tatggactgg gaaatattcg tcattatttg gaaaacgtgc catgactacg
     2281 tttgaacgat actttattgc agataaagct tctcataaag aagaaaaaaa tccatactat
     2341 catctacgtg aagatgtgaa tatggttcgt aaaatgctca gtgattttgg attaaatgca
     2401 gatgaaggtc gcattattaa tggtcacaca ccagtgaaag aaatcaatgg cgaagatcct
     2461 atcaaggctg atggaaagat gcttgtcatt gatggtggct tttcaaaagc ttatcagtca
     2521 acaactggca ttgcaggcta tacactattg tataattcat tcggtatgca actcgttgca
     2581 caccaacaat ttaacgctaa agaaaaaata ctttccgaag gtatcgatga actttctata
     2641 aagcgcgtcg tagataaaga attacaacgt aagaagattc gtgataccaa tattggtaaa
     2701 gatcttcaag ctcaaataga tattttgaaa atgttaatgc atgatcgcta cttagactaa
     2761 agacgacatt ttatgcatgt attgtatact gttgtattga tgcggtttac gcattggcta
     2821 cgcaaacgcg atgataaata ggcgacctag ctacaccgaa aaaaatcccc tcactattgc
     2881 gagtagtgag gggattggtg tatctatgct aattcctgtt aactgtatta taacaacttt
     2941 acagcaaaaa aatgcaaaat caactaataa attaatgaaa tgaccgacgc cctcgcttct
     3001 acttcaattt aacaaaatac tatattagta ctatattttt cattaacatg tatgttacat
     3061 tttctatatt acttaaaatt acgaagggac atttttaaat catgcgtatc ttaaaagagt
     3121 ccattattgt ggcatttgcc tttgttggtg ttgtcgttgg tgccggcttt gctactggtc
     3181 aagaaatttt ccagtttttc acaagtcatg gcgcatatag catttcaggc attattgtaa
     3241 caggactatt gattacttta ggtggaatgg ttgtcatgca tacaggtcat catctaaagt
     3301 ccagaaatca ttctgattca attaactatt tcttataccc ctctattgca agaggttttg
     3361 atattatttt gacaatgttt atgttgtctt tagctattat tatgactgca ggtggtgcgt
     3421 caaccattca tcaaagtttc aacttaccgt attggctgag cgcactcata ttagtcgcct
     3481 ttattttagc aacactgttt ctaaaattcg atcgtttaat tgctgtgctt ggcggtgtta
     3541 ccccattttt aattgcgatt gtcattatga ttgcggtcta ctatttcaca acaagtcatc
     3601 ttgattttac tgccgctaat aatgatgctc agattcataa gcagaaatca ttatcacctg
     3661 gatggtggtt tgatgcgatt aactatgcaa gcttgcaaat tgctgctgcc ttcagcttct
     3721 tatcagtgat gggtagtaaa gttaaatatc gtgactcaac gttatacggg ggcttgattg
     3781 gcggtttaat cattacattt ttactcatga tgattaacct aggtttaatt tctcaattcg
     3841 ataaaattaa acacgtcgat ctacctacat taaaattagc gacacaaatg tctccgtcaa
     3901 ttggtattat tatgtctgtc attatgatac ttgtcatcta caatactgtt gttggattaa
     3961 tgtatgcatt tgcgtcacgt ttcagcgttc catttagcag acgttacttc atcattatta
     4021 ttacaatggc tgtcatcact tatattagta catttatcgg tttcatttca ttaattggaa
     4081 aagtattccc tattatggga ttgttcggtt tcatcttact catacctgta ctctataaag
     4141 gtttaattaa gcgtattacc ggcaaatctc atatcgatta aaatatctat actattgtaa
     4201 aatttagtac aacacttcaa aatcaaaaaa gcgggcagat gaaaagtaaa aatcttttcc
     4261 atctgtccgc ttcttaatag aactgttatt tcccaagtat ttgttgacca gccgttaatc
     4321 cagtttctgt aatttcatga ccattcaccc aaacttcttc gactactgcc ccacgtgtct
     4381 taaacaagtt aatgacttgt tcacttgcag ctaatggcac aattggatca tgtttcccca
     4441 tagaaagcaa cacacttaca tctgataaat cctttgttga cgttacttca actgggtata
     4501 acggtgcata taacaatgct tttttaaatg gtgcttccga acgcaacatt aagttaatcg
     4561 ctatatttga tccatttgaa aatccaacaa gtactgcttt ttcaatatca aattcataac
     4621 gttcagcagc ttctttaatg aacgctaaca attcttgtcc acgaaattcc aaatcttctt
     4681 catcataaac accttcacca agacgtttga aataccggtt catcccattt tctgaaactt
     4741 gtcctctaat acttaacaag tgataatttt cattcaatgc ttcgcctaat ggcaataaat
     4801 cgaattcatc gccacctgta ccatgcaaca atattagcgt tggcgcacct ttttgtcctt
     4861 cacgaaaaat atgttccatc acaaatcctc ctcattccaa ttaatcatgt taacgcttag
     4921 tattaaatgg tcttatttct gattctatat actctctttt attttctaaa aatggtggca
     4981 atgataaacc ttcacctaat gtttcatacg gttcatcttc cataaatcct ggtccatctg
     5041 ttgaaatttc tattaaaata tgccccacac gtgcatataa tgcttcaaaa tagaaacgat
     5101 taacgatgcc tgagttgtta atacctacct ctttatattt cgttgcccac gcttctattg
     5161 catcatgatc tttcacacga aatgacacat gatgtacctc accataacct tgacgtgctg
     5221 ctggcccttt atcatctttt attaagatta cctggccacc attgcctcct tcgccaactt
     5281 caagtaatgc gacattatct tcatgcgcaa tagttgtcat accgtaaaca gtctctaaaa
     5341 tatttttaaa gtcgtcaaaa taacttactt taatttcaat gggtcctaat ccataaatcg
     5401 ctttatctac tggaaccggt ccattcttcc aaggtacacc aggtgctacc ccttcattta
     5461 actcatctga aattaattga tacacttggc catcgacttc ttcaaatggc aacacttttt
     5521 taccaaataa ttcttgaata ccttcgtgtt taacaccaaa ttcatcaaag cgctgttcat
     5581 aatacattaa tgcgtcatcg ttaggcactc taaaagacgg tcttgtaatg gaatttgttc
     5641 ctgcctgccc ttttgtaata tttggaaaat caaagaacgt catgtctgta cctgccgaac
     5701 ctacatcatc tgcaaaaaaa gtatgatacg tataaatatc atcttgattg actgtctttt
     5761 taactaaacg catgcctagt acttctgtaa aaaatttata attacgttct gcatcatctg
     5821 tcattgctgt aacatggtga atacctaata gttcatgatt atttgccatt ttatataccc
     5881 tctttgtctt ttagcatatt tatctcgaaa ccgagatatt ttaatgtaaa tttttagtgc
     5941 acgccttaca cttctgtaga ttgtgcactt aatttcttaa acgcttgttg taagattgtt
     6001 aattcatcct ttgttaacac atcaaacgct tttgttaatg tctcagcatg cttagggaaa
     6061 atatctgcca tttgactttg acctttttca gttaaacaag ccatatatac acgtttatca
     6121 tctttatcct tttcacgtgt aatccaacct ttgtcctcta attgacttac aacatatgaa
     6181 atgctgctac ttgcaattaa tacgcggtct ctaatacgtt gaattggttg cggaccttta
     6241 ttataaagca actcgagcac tgcaaattca gtaatattta agccatatcg ctttacgtct
     6301 tcttttgtaa tttgctctaa tgtgtctaac gccctattca ttccgacaaa gacattgagc
     6361 gattgtttcg ttcgatccat atcatcacca actttttaat ttatttcgat ttcgagataa
     6421 attatatagc taaatgaatt taatgtcaag atacgtgttt tcatcttcat ttcaaactgc
     6481 gcttaataaa aacgactaca taaatgtggc aatgtcgtca catgactgtc atgcaccttt
     6541 gacttcgttt aagttaaaat agaataggga aatactataa tcaatcatga taggagttga
     6601 tatgtatgag taaccttgaa attaaacaag gcgagaacaa attctatatt ggtgatgatg
     6661 aaaatcatgc tttagctgaa atcacatacc gttttgtgga taataatgaa attaacattg
     6721 atcatacagg cgtatctgat gaactcggtg gtcaaggtgt tggcaaaaaa ctagttaaag
     6781 cagttgttga acatgctcgc gaaaatcatt taaaaatcat tgcctcgtgt tcatttgcca
     6841 aacatatgtt agaaaaagaa gattcatatc aagatgtata tcttggttaa ataaattttg
     6901 cgatggtgca actgtgccat cgctttttat ttgtacgaat caatataaaa ttgcaaaacg
     6961 acaagttatt ttgcatttca aactttatgc tatcaattca ttttcttttc tggtatactt
     7021 tttatactaa cttatagaaa aatagaaaac gaggcgatgc ataatggcat ttcacgataa
     7081 aacagcaaca caagtgacaa acattgaact gaacgtaaga gacttagact taatgacaac
     7141 attttacaaa aacattttag gactatccgt taaaagttct gacgataaca caaccgtatt
     7201 atctgttggg actggtggcc atactctaac gttacattta ttagaagacg gccgtcagac
     7261 ttccccacgt gaagcagggc tttttcatat agcactttta ttaccaacta ctgaggatct
     7321 agctaacttc ttatatttcg tggcacaaaa aaacatgggc atcggcgctg gtgatcattt
     7381 agtaagtgaa gctttatatt tcaacgaccc cgaaggtaat ggtattgaag tctatcgcga
     7441 tagaccttca tcttcatggg aatggcaaaa tggcaaagta aaaatggata ctttagaagt
     7501 tgatagccaa actttattaa cacatcgtac tgacgaaggt tggcaaggaa tgccagcaaa
     7561 aggcatgatt ggacacttac atttaaaaac acatgatttg gacgcagcat atcaatgtta
     7621 cattgaacaa ttaggattcc aacatgtgtc tgattttcca cgtgcgttat ttatgtcaac
     7681 gaatcattat caccatcata ttgcgtctaa cacttggcaa tccaatcaag caagacaaaa
     7741 caatgcacaa agttatggat taacacatgt tgatatttat caacccgacg cctcaacaca
     7801 tacatttacg gcaccagaag ggtttgacat cacagttcat tcaaatactg accttgttcc
     7861 agaaaaataa tcatccatac tttattaata caattttttc acgcaaaagc ccgcgattca
     7921 tttatatgaa cgcaggcttt tattattgct aactttttaa ttatatatat ttagacatgc
     7981 tgtattctat ttattctttt ggtccaaccc attcaataac atcttcatat gcttggcgtg
     8041 ttttattttt cggtggatct tgttgtctgt agccaaatgc aaccatcact gataaaccaa
     8101 attgctcagt atctaagata cctttatttg ctaaaatgtc tgtcactgta tccagactaa
     8161 aaccttccat cggacatgaa tcaataccta acaatgcggc tgtcgtcatc atattgccta
     8221 atgcgatata cgtttgttta cttgaccagt catacaaggc ttgatcatta tcagaaatat
     8281 ggaaatctgc ttggaatgca tcgaattttt gttcaacagc tgggatcgtt tgtgcctcat
     8341 attttttaat atctcttaac atatgttgta catacggtga tcttgacgtt acatttttac
     8401 gcgcaaaaat tagcacaaaa tggctcgctg tatctaattg ttttgctgcg ccccaactgt
     8461 gcgcttttaa ttcatcacgt aacgcttgat cttgaatcac gacaaacttc caaggttcta
     8521 acccaagaga acttggagac aatctacctg actctaatat tgtttcaaaa tcttctttcg
     8581 aaactttctt ttgtggatcg aattgcttag tcgcatgtct gaaatgaaat gcatccataa
     8641 ttgtttgatt catattgctc attgaacgta gcctccaatt tttatattta ttactataca
     8701 aattatactt catgttttaa aaagactaaa gtaatccgtt tgcaattcac tataacttgt
     8761 gcacatgtta taagttttct gcaataaaac acacttttga attttggaaa atttagtgtt
     8821 ataatatatt tgtgatattt ttcacaatta aattgtggcg tgattttaat gtacataatc
     8881 tttaatttca ctcatttact tttcaatctt ttaaaagcga ggtttttaat aatgacaaaa
     8941 attatgttct ttggtacgcg tgattatgag aaagagatgg cattaaattg ggggaaaaag
     9001 aataatgtcg aagtaactac ttctaaagag ctattatcaa gtgctacagt cgatcaatta
     9061 aaagattacg atggcgtaac tacaatgcaa tttggtaagt tagaagatga cgtttatcct
     9121 aaattagaat cttacggtat taaacaaatt gcacaacgta ctgctggatt tgatatgtat
     9181 gatttagatt tagctaaaaa acacaacatt gtgatatcta acgttcctag ttattcacct
     9241 gaaacaattg cagagtattc tgtttctatc gccctacaat tagtgcgtcg cttcccagat
     9301 attgaacgcc gtgtacaagc acatgatttt acttggcaag cagaaatcat gtctaaacca
     9361 gttaaaaata tgactgttgc aattatcggt acgggtcgta tcggtgctgc tactgctaaa
     9421 atatatgcag gatttggtgc tacaattaca gcttatgacg cttatcctaa taaagattta
     9481 gactttttaa cttataaaga tagtgttaaa gaagctatta aagatgccga tattatttct
     9541 ttacatgttc cagcgaacaa ggaaagctat catttattcg ataaagcaat gtttgatcat
     9601 gtgaaaaaag gtgcaatttt agttaacgca gcacgtggtg cagtcatcaa tacacctgat
     9661 ttaatcgctg cagtgaacga tggtactttg ttaggtgctg cgattgatac ttatgaaaat
     9721 gaagcagcat acttcacaaa tgactggact aataaagaca ttgacgataa aacattatta
     9781 gagttaatcg aacatgaaag aattttagta acaccacata ttgctttctt ctctgatgaa
     9841 gcagtacaaa accttgttga aggtggttta aacgcagcat tatctgtcat taacactagt
     9901 acatgtgaaa cacgtttaaa ttaatatata tttaaaagca aaggcgcgcc atgtgccttt
     9961 gctttttatt ttgtatatct gtataaaaac cagtttgatt aaaatccgct ttattttatc
    10021 atctattcaa atgattttag tgcgcttatt ttactatggc attattgcct aacgtgtttg
    10081 agatatatta atatttgatt attcgtattc aaagttttca tttaacatgt atttagtatc
    10141 ataatagccg cttcattgat gatgtactac ctacttgttc aatacttctt gttcgataat
    10201 cgttaaaaca ccctgcttat cattacttgg agcgacagca gaggcaatct tgaacaattc
    10261 ttcatcatga ctattttcca tcacataact atgcttagcg aacgcaagca tatctttatc
    10321 attattcgca tctccgaagg ccatgagctc tgaagaagac attccccatt tatctaacaa
    10381 tcgttttaat gcctgcccct tagtcatgtt tggcataata atatcaatgc tatcgtgccc
    10441 acttgagaca agtttaatat cattgctgaa ttgcgttgct acttcttcgt caacatttgg
    10501 atgcgtctca cgattaatat taaatgctat tttgacataa tcatcctcag gtaattgttg
    10561 tagactgtca atttctttta actgatgata ataaaatctc gtatcttctt taaatgcttc
    10621 agaagtatgt tttaaaatat acgcgctttt caaaccacag atgacgagtt gatcaatact
    10681 ttggttcata tttaaataat cgacaacctg ttgaaacacc tgacgattaa agcttttata
    10741 attatataac tcattgccat tataaataac tgcaccattt tcagaaataa aatacatatc
    10801 tctatccccg aaaatagact tcaacttcgc atattgattg ccactcgcag caataaatgt
    10861 aatatctcta tttctaagtt cagtaaaaat cgcttcaaat cttgatttat cgtatgtctt
    10921 tttcgaatct aaaaacgtcc cgtccatatc taccgcaata gccttaacca tgattatcct
    10981 cctcattgct cttttagtcg tcttcattat atccatctca tgacaagcaa tgaagtttgt
    11041 cataccacaa tgaacctata ttcacatttt gaaattgctt tcatatatgc aaaacacatt
    11101 acactggata tagcattcag aggcgaacat aaatttcaga atattgtaaa aatataacga
    11161 tatatttatc atttggacaa aatatagtgt gatatactaa tttataacat ctatacataa
    11221 tgtgaaagaa tttttaaaaa tacaattaaa tatcggtgta ataaatccct ttatacatag
    11281 gagttgtata catgaaacta aataactatt ctttaaaagt taaaaacaaa caactagttg
    11341 acaattgtga tttaaatttc tatcttggtc agatcaatca cattgttggt aaaaatggtg
    11401 taggaaaatc tttattagct aaagatttct tactaaataa tagtggaaat atccctaagt
    11461 ccatttctca aaatgtaacc ttaatatcaa gttcatcaaa tattcctaat gatataacaa
    11521 aagatttttt attatcattg ttaaaatcaa aatttgaaaa caatcgacaa acattcgata
    11581 agatttataa catactaaac atcgaagcaa taccgtctaa cgtactacta aaaaacttga
    11641 gtgatggtca aaaacaaaag cttaaattat taagtttctt attggaagat catgatttaa
    11701 tcatattaga tgaagttaca aacgctttag acaagaaaac agttaatgaa atttatgaat
    11761 ttttaaatga ttttattcaa agccatcaaa ctaaaactat tatcaatatt acacataatt
    11821 tatccgattt aagtgctttg ccgggtaaat actttatttt taaagacctt caaatagaag
    11881 agtaccaatc aaaagaataa gtcataaatg attacattaa tttataattt aggtgtccac
    11941 tttgaaatta gaatttaaaa aaagtatttc caataaaatt atttacacgc ttggtgtgct
    12001 attcatattc cttttcttac ttgggtattt tttaccaata ggaatagaca aagttaaaag
    12061 tttaagctat agccagtttt tcttcagttc atatactgtt gcaacgcaat tagggttctt
    12121 actattttca tttgttattg catattttat taataaagag tattcgaata aaaataccct
    12181 gttttataaa ttgattggcg ataatatctt taccttcttc tataaaaagg tagcagtgtt
    12241 attttttgaa tgtttagtgt tcataattct aagtatcaca attatttcaa ttatatattc
    12301 ggatttctca cattacttac tattaataat cttattttca ctcgttatct tacaatatat
    12361 cttagttgtt ggaacgatta gtatggtttc acctaatatt ttaatcagct taggtatcag
    12421 cattgtatat tggattggtt cagtcatctt agttgccatt aataaaaata tatttggtat
    12481 cgttgctcca tttgaggcga gcaatacgat gtatcgtgcc gtagaaaaaa tattgaataa
    12541 cgagtcaaca tttatgtgtc ctactgaaat tataaataca gtatcattct tcgttcttct
    12601 atttatagtt aatactattg tcttattact aagtagaaag agatggttga aaatcggtat
    12661 gtagtacata aaaaggagcg gcatccattg tcgctccttt ttattatgta tttttacaat
    12721 ctccaccaat gacacgtcgt cattgctacc tcataccaaa caaaaaaaca gtctcgcaat
    12781 caagactgtt ttccactcaa tatattcatc cattagcgta atagattatt tgacttctgt
    12841 agctacaaag attttacgtt tttcccaaac gcctgtcttt tcattgtaat catcacaagt
    12901 aattaatgtt aattgtttat ctttaccttt ttgttcatct agaacttcta catctgtagg
    12961 cttaacatct cttatacttg tcattttata cttacgtgtt tcattaccaa ctttaaagta
    13021 caccatacta ccttttttgg ctgctttaag atttgtaaat tgatagttcg gacggtcaat
    13081 gaaagtgtgt cctgcaattg aaatattttg atcatctagt gattcgtttt cttctgcaaa
    13141 gcttacacct ctatttaatt gttcaggtgt tgctggtcct ggatatactg gttctttaat
    13201 atcagcatct ggaatttcaa tatagcctgc cactttcgat ttatctttcg gaatttgagg
    13261 tttagcttgc tgctttttat ctttactcgc ctgttctttt acatttttat catattgttc
    13321 aatcttttca tctttatctt tatcgtgaag ataattatcg atatgtggtt tagcaaacaa
    13381 atatgctgcc actaggataa gtaccacacc agcgattgtc attaatcgat ttgtccattt
    13441 tttcatacgt taaggctcct tttatacatt tcacaatgcc tgttaacatt tcatatagta
    13501 taccattaat ttcaaaatga ctcatagcaa ttcattttat actataaaat ttacatgtat
    13561 acttttacgt tagatttcat tacacatatt tgcattcaaa taacgaaacg cttttaataa
    13621 ttactaaggg ggaattgata tgattagata cgctaaaaaa gaggatttaa acgctatatt
    13681 agcgatatac aatgatgcca ttatcaatac tacagctgtt tatacttatg aaccacaaac
    13741 catagacgaa cgtgtcgcat ggtttgaaac gaaacaacgt aagcatgagc ctatctttgt
    13801 atttgaggaa aatggaagtg tcttagggtt tgcgacgttc ggttcgttta gaccttggcc
    13861 agcatatcta tatacaatcg aacattctat ttatgtcgat gcttcagcta gaggaaaagg
    13921 tattgctagt caattactac accatttaat tgttgaagca aaagctaaag gttatcgtgc
    13981 gctagttgca ggcattgatg cttccaacaa agcgagtatt cagttgcatc aaaagtttgc
    14041 ttttaagcat gccggcacac tgaccaatgt aggttttaaa ttcaatcaat ggttagattt
    14101 agcattttac gaattagatt tacaagacta gtaatgtttg aatcacataa tataaacaag
    14161 acaaccatgt taattccctt aacataacaa gccaacatat aaaattttaa acttctca
//
LOCUS       DBNENW010000061        13942 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNENW010000061 DBNENW010000000
VERSION     DBNENW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.061"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            60..2333
                     /gene="recJ"
                     /locus_tag="AC1LW8_001457"
     CDS             60..2333
                     /gene="recJ"
                     /locus_tag="AC1LW8_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIP4773294.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     AIVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2355..2873
                     /locus_tag="AC1LW8_001458"
     CDS             2355..2873
                     /locus_tag="AC1LW8_001458"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIP4773295.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            3301..5490
                     /locus_tag="AC1LW8_001459"
     CDS             3301..5490
                     /locus_tag="AC1LW8_001459"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIP4773296.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            5502..5954
                     /gene="dtd"
                     /locus_tag="AC1LW8_001460"
     CDS             5502..5954
                     /gene="dtd"
                     /locus_tag="AC1LW8_001460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIP4773297.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            5951..6826
                     /locus_tag="AC1LW8_001461"
     CDS             5951..6826
                     /locus_tag="AC1LW8_001461"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIP4773298.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            7286..8548
                     /gene="hisS"
                     /locus_tag="AC1LW8_001462"
     CDS             7286..8548
                     /gene="hisS"
                     /locus_tag="AC1LW8_001462"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIP4773299.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            8564..10330
                     /gene="aspS"
                     /locus_tag="AC1LW8_001463"
     CDS             8564..10330
                     /gene="aspS"
                     /locus_tag="AC1LW8_001463"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIP4773300.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            10408..10598
                     /gene="ssrS"
                     /locus_tag="AC1LW8_001464"
     ncRNA           10408..10598
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC1LW8_001464"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10663..10791
                     /locus_tag="AC1LW8_001465"
     CDS             10663..10791
                     /locus_tag="AC1LW8_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773301.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            10791..11564
                     /locus_tag="AC1LW8_001466"
     CDS             10791..11564
                     /locus_tag="AC1LW8_001466"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIP4773302.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(11725..12999)
                     /locus_tag="AC1LW8_001467"
     CDS             complement(11725..12999)
                     /locus_tag="AC1LW8_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIP4773303.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            13084..13506
                     /gene="cymR"
                     /locus_tag="AC1LW8_001468"
     CDS             13084..13506
                     /gene="cymR"
                     /locus_tag="AC1LW8_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine metabolism transcriptional regulator
                     CymR"
                     /protein_id="HIP4773304.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            13606..13788
                     /locus_tag="AC1LW8_001469"
     CDS             13606..13788
                     /locus_tag="AC1LW8_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIP4773305.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            13828..>13942
                     /locus_tag="AC1LW8_001470"
     CDS             13828..>13942
                     /locus_tag="AC1LW8_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAS049 family protein"
                     /protein_id="HIP4773306.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNE"
BASE COUNT         5062 a         1923 c         2702 g         4255 t
ORIGIN      
        1 gctttttatt taaagtaatt ttagatataa tgtcttttgg aaatgaggcg taaatatata
       61 tgattaaacc gaagtataag tggaaattaa ctaaaccagc tgaatacata agtgatgaat
      121 taacatcaaa attaaagtta acgccaatcg taaaaaaaat attagaaagt aaatcgatta
      181 ttgatgaaca agcaatagaa tccatcattt cagatacaga tatcaatcat gatgcattgc
      241 aattgagcga tatgactaag actattgaaa gaattaaaag agcgatagcg aatgatgaga
      301 aaatcctagt ttatggtgat tacgatgctg acggtgttac ctcaactaca attttagtga
      361 caacattaca acttttaggt gcacaagtag gatggcatat tcctaaccgc tttaccgaag
      421 gatatggacc aaacgaacta gcatttagaa atgctcatga tgaaggcata acattaatca
      481 taactgttga taatggcata caaggtcata atgaaattaa aatggttcaa gatttaggag
      541 ttgatgtcat tgtaactgat catcatgaaa ttggtagtac attaccagaa gcatatgcaa
      601 tcgtacatcc aatgcatcca tcgtttaatt atccgttcca acaattgtgt ggtgcaggtg
      661 ttgcatacaa attagcacaa gcattgattg aaaatgtacc tgattatttt aaagcaatag
      721 ttgcgatagg taccattgcg gatttagtat ccttaactga tgaaaatcga tcattagtga
      781 aacaaggatt aaaagtctta aatgatcaat gtccgacctc tgttaaagca ttgctaaaag
      841 aagcgggata caatgacaat attgatgaag aaacaattgg ttttattatt ggacctagac
      901 ttaatgctgt aggtcgttta gatgacgcat cattagcttg cgaattgttg atgactgacg
      961 acgaagaaga agctgcattt ttagcagaac aagttgaaca ttttaaccgt gaaagaaaag
     1021 atattgtagc aactatcaca gaagaagcaa tggctatggc agaaacgaaa gttaaaaagg
     1081 gagatttatt tttactttta gcaaaagaaa attggcatga aggtgtctta ggtattgttg
     1141 cttcgaaaat tgtcgaaaca tttgctttgc caacattaat tttaaatatt gatagggagc
     1201 aaaatcatgc aaaaggttcc gccagatcaa ttgatcaagt gtcgatgttt gaaatactat
     1261 cagcacatca agagttaatc gctaaatttg gcggccatca tatggctgca ggtatgacga
     1321 tggatattga aaatatagaa tcgttagctg aaggtttaaa taaatggatg aaagaattat
     1381 ctaaaacaac ttcgttagat cccgtaaagc aagtagatgt attattaaca gaaaatgata
     1441 taacgattaa aaatattcgt gatatgaatc gtttaagacc cttcggaaca gatttttcta
     1501 gacctatttt tgaaatggat gatttatcag tttcttcagt taaggcgata ggtcaacaaa
     1561 aaaatcatct aaaattgaca cttggtgaaa gtaatatcgc cgcgctattt tggcaaaatg
     1621 gacacttaga acctgagtta caagatgaac agccaattaa tatattagga tctgttcaaa
     1681 tcaacgaatg gaatggtaat caatcaccgc aaataattat tcaagatatt gcgatgaatg
     1741 aacagcaaat attagattat agaagtaagc gaaaaagttt accttttaca gaaaatgatg
     1801 aaaatattgt cgtgcttatt catcctaaaa gtgataaagt aaatgcgaat gaatattatt
     1861 atggtgaaga aattaaacaa caaactgata aagtagtatt aagagattta ccaacgtcaa
     1921 tggaagactt gtctaattcc ttgcaacaac tgcaattttc tcaactttat atagttttgc
     1981 aacataatca ttcgatttac ttcgatggta tacctaatat ggatattttt aaaaagtgtt
     2041 ataaagcatt aataactaaa caagaaacaa atatccagaa agagggtatg ttattgtgtc
     2101 aacatttaag tgtgaaacca gatacactta aattcatgtt gaaagttttc ttagacttaa
     2161 aatttgtaac acaagaagat ggtttaattc gaatcaatca acaacctgat aaaagatcga
     2221 ttgattccag caaagtatat caattaagac aacaacgtat ggatgttgaa aagcaattat
     2281 tatatcaaga tttttcagaa ataaaaaatt ggataaagtc acaattgtcg tgagcaattt
     2341 aggaggaaat attaatggat ttaaagcaat acgtatcaga agttcaagat tggccgaaac
     2401 caggtgttag tttcaaggat attactacaa ttatggataa tggtgaagca tatggctatg
     2461 caacagataa aattgtagaa tacgcaaaag acagagatgt tgatatcgtt gtaggacctg
     2521 aagcgcgtgg ctttatcatt ggctgtcctg tagcttattc aatggggatt ggctttgcac
     2581 ctgttagaaa agaagggaaa ttacctcgtg aagtcattcg ttatgagtat gacctagaat
     2641 atggtacaaa tgttttaaca atgcacaaag atgcaattaa accaggtcaa cgtgtgttaa
     2701 ttacagatga tttattagct actggtggta cgattgaagc agcaataaaa ttagttgaaa
     2761 aattaggcgg tatcgtagta ggtattgcat ttataattga attgaaatat ttaaatggta
     2821 ttgaaaaaat taaagattac gatgttatga gtttaatctc atacgacgaa taataaataa
     2881 tataatttta tcaaatgaaa tccttcatca aatgtataag aaccaatgac ttaattaaaa
     2941 aagttgttta agttttctta acatgagatg ttaggatttt ttatttactg aaaatgttag
     3001 atgattgagc attatacctt aataacatcg tttatttatt tcataaattg tagtatcata
     3061 gaactaatat ttaaaaaatg aaacagtaga tttaggtcga atttttgtaa aagttttaaa
     3121 agtaggaata gtatacaaat taaactcgct caagtaaaat taatattacg attaatgacg
     3181 acaggataaa tatttatcgt cgacggacgt atgattggtg tgggacaaat actattcaac
     3241 aagagtacct aaatcattgt ttaaggcgaa gtaataaata tgaatggggt gtatcatata
     3301 atgaacaacg aatatccata tagtgcagat gaagttcttc acaaagcaaa atcatatttg
     3361 tcagcagatg aatatgagta tgttttaaaa agctatcata ttgcttatga agcacataaa
     3421 ggtcagttcc gaaaaaacgg attaccatac attatgcatc ctatacaagt tgcaggtatt
     3481 ttaacagaaa tgcgattaga cggaccgacg attgtcgcag gttttttgca tgatgtaatt
     3541 gaagatacac cgtatacatt tgaagatgta aaagaaatgt tcaatgaaga agttgctcga
     3601 attgttgatg gtgtgacgaa gcttaaaaaa gtaaaatacc gctcaaaaga agaacaacaa
     3661 gctgaaaatc atcgcaagtt atttattgcg attgccaaag atgtacgcgt aattttggtg
     3721 aaattagcag acagattaca taatatgcgt accttgaaag ccatgccgcg tgaaaaacaa
     3781 attagaattt ctcgagaaac attagaaatt tatgcacctt tagcacatcg tcttggtatt
     3841 aatacaatta aatgggaact agaagatacg gctcttcgtt atattgataa tgtgcaatat
     3901 tttagaatag tcaatttaat gaagaagaaa cgtagtgaac gtgaagcgta tatcgaaacg
     3961 gctattgata gaatacgtac tgaaatggac cgaatgaata tcgaaggcga tataaatggt
     4021 agacctaaac atatttacag tatttatcgg aaaatgatga agcagaaaaa acaatttgat
     4081 caaatttttg atttgttggc gatacgtgtt attgtcaatt ctattaatga ttgttatgcg
     4141 atacttgggt tggtgcatac gttatggaaa ccgatgccag gacgttttaa agattatatt
     4201 gcaatgccta aacaaaattt gtatcagtca ttgcatacta cagtagtagg tccaaatgga
     4261 gacccgctcg aaatccaaat acgaacgttt gatatgcacg aaattgctga gcatggtgtt
     4321 gcagcacact gggcttacaa agaaggtaaa aaagtaagtg aaaaagatca aacttatcaa
     4381 aataagttaa attggttaaa agaattagct gaagcggatc atacatcgtc tgacgctcaa
     4441 gaatttatgg aaaccttaaa atatgactta cagagtgaca aagtatacgc atttacccca
     4501 gcgagtgatg ttattgagtt gccatatggt gctgtgccga ttgattttgc ttatgcgatt
     4561 cacagtgaag taggtaataa gatgattggt gccaaggtga atggcaaaat tgtaccaatt
     4621 gactatattt tacaaacagg cgatattgtt gaaatacgta ctagtaaaca ttcatatgga
     4681 ccaagtcgtg attggttgaa aattgttaaa tcgtctagtg ccaaaggtaa aattaaaagt
     4741 ttcttcaaaa aacaagatcg ttcatctaat attgaaaaag gccgaatgat ggttgaagtt
     4801 gaaataaaag agcaaggatt tagagtcgaa gatattttga cagagaaaaa tattcaggtt
     4861 gttaatgaaa aatataactt tgcaaatgaa gatgatttat tcgcagctgt aggatttggc
     4921 ggcgtgacat ccttacagat tgttaataaa ttaactgaaa gacaacgtat tttagataaa
     4981 caacgtgctt taaatgaagc acaagaagtt acgaaatcat tgcctattaa agacaacatc
     5041 attactgata gtggtgtcta tgtagaaggt ttagaaaatg tacttatcaa gttgtcaaaa
     5101 tgttgtaatc ctataccagg tgatgatatt gtaggttata tcaccaaagg tcacggtatt
     5161 aaagtacatc gcactgattg cccaaatatt aagaacgaaa ctgaacgact aattaatgtt
     5221 gaatgggtaa aatcaaaaga cgcaactcaa aaatatcagg ttgatttaga ggtaactgcg
     5281 tatgaccgaa atggcttgtt gaatgaagta ctacaagctg ttagctcgac agccggcaat
     5341 ttaattaaag tttcaggacg ttcagatatt gataaaaatg caataataaa tattagtgtc
     5401 atggtgaaaa acgtgaatga tgtttatcgt gtggtagaaa agatcaaaca acttggtgat
     5461 gtttatacag taacaagagt ttggaactag aggtgcaaaa tatgaaagta gttgtacaaa
     5521 gagttaaaga agcatcggtg acgaatgata cattaaataa tcaaatcaaa aaaggatatt
     5581 gtttattagt cggtatcggt cagaactcta cagagcaaga tgcagatgta attgcaaaga
     5641 aaattgctaa tgcaagatta tttgaagatg acaataataa attaaacttt aatatccaac
     5701 aaatgaatgg tgaaatacta tcagtttcac aatttactct ctatgcagat gtaaaaaaag
     5761 gtaaccgtcc aggtttctca aattctaaaa atcctgatca agcggtaaaa atttatgagt
     5821 attttaatga tgcgctacga gcgtatggtc ttactgtgaa aacaggtgaa tttggaacac
     5881 acatgaatgt tagcataaat aatgatggtc cagtcactat tatttatgaa agtcaggacg
     5941 gcaaaattca atgaaaaaaa tagaggcatg gttatctaaa aagggtctta aaaataaacg
     6001 tactctaata gtagtgattg cctttgtctt atttatcatc tttttatttt tattgctgaa
     6061 tagcaatagt gaagatagtg ggaacatcac gataactgaa aacgctgaat tacgtacagg
     6121 tccaaacgct gcgtatccag tcatatataa agttgagaaa ggtgaccatt ttaaaaagat
     6181 tggtaaagta ggtaaatgga ttgaagttga agatacatcc agtaatgaaa aaggttggat
     6241 agctggatgg cacacaaatt tagatattgt cgcggataat acgaaggaga aaaatccttt
     6301 gcaaggtaaa acaatagtgc ttgatcctgg tcatggaggt agtgaccagg gtgcttcaag
     6361 caatactaaa tataaaagtt tagaaaaaga ctatacgttg aaaacagcaa aagaattgca
     6421 gcgtacttta gaaaaagaag gcgcaactgt taagatgaca agaacagacg atacatatgt
     6481 ttcactagaa aatcgtgata tcaaaggcga tgcctatttg agtatacata atgatgcatt
     6541 agaatcatct aatgcaaatg gaatgacagt ttattggtat catgataatc aaagagcttt
     6601 agcagatacg ttagacgcta cgattcagaa gaaaggtcta ctttctaatc gcggttcaag
     6661 acaagaaaat tatcaagtgt taagacaaac aaaagttcct gctgttttat tagaattagg
     6721 ttatattagt aacccaactg atgaaacgat gattaaagat caattacata gacaaatttt
     6781 agaacaagca attgttgatg gccttaaaat ttatttttct gcgtagggct tgcaaaaata
     6841 tgtgaaagta gttatcattg atattgaatt ttataactaa aaccgttagt attcttgaaa
     6901 tggtaaatga aataggtagc aatctaacta agattgtgta ggaatataat ccatagactg
     6961 aaagattatg ctgagtagtt tatatacatt gaacacaaga agaggtgctt tatgaaaagt
     7021 aaagccgtta aacgtacgtt aaacgttttg agtgggttta tttaatgcac gcttataaaa
     7081 agtaatgatg attacaatta ggcatgtttt taaaccaatg agggtggcaa cacggatatt
     7141 cgtcccttgt gcagtttcag gctgtgcaag ggctttttct tttgctttta aaattatgat
     7201 ttatcatgca aagttaagtg atcgtatata gttttagttt taaaaaggta attaaataaa
     7261 atagtttgcc gagggagatg tcaaaatgat taaaatacct agagggacgc aggatatttt
     7321 acctgaagat tcaaagaaat ggcgttacat tgaaaatcaa ttagatgaat taatgacatt
     7381 ttataattat aaagaaataa gaacaccaat ttttgaaagt acagatcttt ttgcaagagg
     7441 tgttggtgat tcaaccgatg tcgtacaaaa agaaatgtat acatttaaag ataaaggcga
     7501 tagaagtatt acattaagac ctgagggaac agctgcagtt gtgcgttcat atattgaaca
     7561 taaaatgcaa ggtaatccaa accaaccaat taaactttat tacaatggac cgatgtttag
     7621 atatgaacgt aagcaaaaag ggcgctatcg tcaatttaat caatttggtg tagaagctat
     7681 tggtgctgaa aatcctagcg tagatgcaga agtattagct atggttatgc atatttatca
     7741 atcatttgga ttaaaacatt taaagcttgt tattaatagt gtaggggata tggcgtctcg
     7801 aaaagaatat aacgaagcgt tagtgaaaca ctttgaacca gtaattcatg aattttgttc
     7861 agattgtcaa tcacgtttgc atacaaatcc gatgcgaatt ttggattgta aagttgaccg
     7921 tgataaagaa gcgattaaga ctgcacctag aatcactgat ttcttaaatg aggaatctaa
     7981 ggcatattat gaacaagtaa aagcttattt agatgattta ggtattccat atattgaaga
     8041 tcctaactta gttcgtggat tggattatta tacacataca gcatttgaat taatgatgga
     8101 taaccctaac tatgatggtg ccattacaac gctttgtggt ggtggccgtt ataatggttt
     8161 attagaattg ctagatggtc caagtgaaac aggtattggt tttgcgctaa gtatagaacg
     8221 attattgctt gcacttgaag aagaaggtat cgaattagat attgaagaaa acttagattt
     8281 attcattgtt acaatgggtg atcaagcaga tcgatatgct gtgaagttat taaatcattt
     8341 gagacataat ggtattaaag cagataaaga ctacttacag cgtaaaatta aaggacaaat
     8401 gaaacaagca gatcgtttag gtgccaagtt tacaatcgtt attggtgatc aagaattaga
     8461 aaataataaa atcgatgtta aaaatatgac aactggtgaa tctgaaacaa ttgaattaga
     8521 cgcattagtc gaatatttta agaagtagag agggcgttaa aatatgagta agagaacaac
     8581 ttattgtgga ttagttactg aagcattttt aggacaagaa attacattaa aaggatgggt
     8641 taacaatcgt cgtgacctag gtggattgat tttcgttgat ttaagagata gagaaggaat
     8701 tgtacaagtc gtgtttaatc ctgcattttc agaagaggca ttgaaaattg ctgaaacagt
     8761 acgttctgaa tatgttgtag aagttcaagg tacagttacg aagcgtgacc ctgaaacagt
     8821 taatcctaaa attaaaactg gccaagttga agtacaagtt acaaatatta aagtgattaa
     8881 taaatctgag acaccaccat tttctataaa tgaagaaaat gttaacgttg atgaaaatat
     8941 tcgattaaaa taccgttatt tagatttacg tcgtcaagag ttagcgcaaa catttaaaat
     9001 gagacatcaa attacacgtt ctattcgtca atatttggat gatgaagggt tctttgacat
     9061 cgaaacacca gtactaacga agtcaacacc tgagggtgca cgtgactatt tagtaccatc
     9121 tcgtgttcat gatggtgaat tttatgcatt accacaatca ccacaattat ttaagcaatt
     9181 attgatgatt agtggatttg acaaatacta ccaaatcgta aaatgcttcc gtgacgaaga
     9241 tttacgtgca gatcgtcaac ctgaatttac acaagtcgat attgaaatga gttttgtaga
     9301 ccaagaagat gtgatgcaaa tgggtgaaga aatgcttaaa aaagttgtta aagaagttaa
     9361 aggcgttgaa attaatggcg ctttcccacg catgacatat aaagaagcga tgcgtcgcta
     9421 tggttctgat aaaccagata cgcgttttga aatggaatta attgacgttt ctcaattagg
     9481 acgtgatatg gactttaaag tatttaaaga tactgttgaa aatgatggtg aaattaaagc
     9541 aattgtcgct aaaggtgcag ctgaacaata tactcgtaaa gatatggatg ctttaacaga
     9601 atttgtaaac atctatggtg ctaaaggatt agcgtgggtt aaagttgtgg aagatggttt
     9661 gacaggtcca attggacgtt tctttgaaac agaaaatgtt gaaacattac ttacattaac
     9721 tggtgctgaa gctggtgact tagtaatgtt tgttgcagac aaaccaaatg tcgttgcaca
     9781 aagtttaggt gcattacgtg tcaaattagc taaagaatta ggtttaatcg atgaaacaaa
     9841 attaaacttc ttatgggtga cagattggcc attattggaa tatgatgaag atgcgaaacg
     9901 ttacgttgca gcacatcatc catttacatc tccaaaagaa gctgatattg ctaagcttgg
     9961 cactgcgcca gaagaagctg aggcaaatgc ttatgacata gtattaaatg gttatgaatt
    10021 aggtggcggt tcaatcagaa ttcatgatgg tgagttacaa gaaaaaatgt tcgaagttct
    10081 tggatttact aaagaacaag cacaagaaca gttcggcttc ttactagatg catttaaata
    10141 tggtgcacca ccacatggcg gtattgcatt aggattagac cgattagtca tgttattgac
    10201 taatagaaca aacttgagag atacaatcgc attccctaaa acagcatctg ctacatgttt
    10261 attaacgaat gcgcctggtg aagtttctga taaacaatta gaagaacttt ctttgcgaat
    10321 tcgtcactaa attttaaaaa atcaataaac tttggtttgt tataatttca ttaattttaa
    10381 ttatgtggta atataacatc ataagatagt catctgagtt gttcgtaagt ttgcttttta
    10441 atttgggcct aacactcttt gatcaaggga gcccaatggg ttttcttgca gcgtacacgc
    10501 cttatatgaa ggacgtacaa aacaagaaac agggcaccca cctgtatata acaggccgaa
    10561 tgatcaagct attataacta cggcaatacg gactctatcg atacgcaaga ctttgtcttg
    10621 cgtattttta tgcgtttctt taagttttta ataaagaatt agttgggttt tatagcgttt
    10681 aataaatgtt tcctcgttga attttgggaa aataatcaaa gcgataatgc taaatataaa
    10741 attttaagtt actatgtact tatgtataaa tcagtgaaag aggtaaaata atgaaacatc
    10801 aattttcaag aaatgaacta gcgattggac aacaaggttt agatttattg aagcaacaaa
    10861 ctgttgtcat acttggcgtt ggaggtgttg gttcatttgc tgctgaagca ttagcaagaa
    10921 cgaatattgg tcatattata ttaatagata aagatgatgt tgatattaca aatgtaaaca
    10981 gacaattaca cgctttaact acaacggtag gacaaagtaa agtttctttg atggaagaac
    11041 gtattaaatt aattaatcca gattgtaaag tcacgccctt acatatgttt tatacagaag
    11101 agacatatga agaaattttc gataactacg atatagatta ttttgtagat gcaagtgata
    11161 caataatgta caaagtacac ttaatgaaag agtgtcttga ccgagggatt aaagtgattt
    11221 ccagtatggg tgctgcaaat aaaacagatc caacacgttt tacaatttca gatatctcta
    11281 aaacacatac cgatccaatg gccaaaatta ttcgtcaaaa attgaaaaaa ctaggaattc
    11341 gtaaagggat tccagttgta ttttcagatg aaagtccaat tgtcataaga gaagatgtaa
    11401 aagatatagt tggagataaa aatgctatca atcgaaaagg acaaatgcca ccttcttcaa
    11461 atgcctttgt gccaagtgtt gttggattaa tttgtgctag ttatgtggtg aatgacgtat
    11521 taaaagatat tccagttcgt cgcattaaag acaaagggca ataattcatt ttgaaagtca
    11581 taattttcaa cgtaaggcaa gtgtaaccac accataaaac taaaactgac tagttcgcaa
    11641 gcatagtaga atatgcctcg tatactagtc agttttgatt tgattatagc taaaatatca
    11701 aaggtaatcg atttaatctt atttttacgg cctttgttta agtaagttgt tatatatttc
    11761 tttgagttgt tgttcacttt tagatgtcgt ttttggttca taataaattt tgtttttaag
    11821 tttatcaggt aaatattgct gtgaaacata gccattaaca tattggtgtg gatatttgta
    11881 accaatagat cggcctagat ctttagcacc ttgataatgt ccatctttta aatggtttgg
    11941 aatttggccc acatgaccgt ttctaatatc ggacaatgca ctatcaatgg cactcattgc
    12001 tgaatttgac ttaggtgata agcatagttc gattactgct tggcttagtg gaattctagc
    12061 ttctggtaaa cctagacgtt ctgctgattc aatagcagca agtgttctct gaccagcatt
    12121 aggtgaggct aagcctatat cctcatagct aattacaagt aatcgtcgaa ctattgtagg
    12181 taaatctcca gcttcaatta atcgtgctaa ataatgtaaa gcggcattga cgtcgctacc
    12241 acggatagat ttttggaaag cgctcataac atcgtaatgc atgtcaccat ccttatcact
    12301 tacaaatgca cctttttgta aacagtcttt agcatcttgc aatgtaacat gtcgataacc
    12361 gtctttgtca ttatctgcgc ttaatacagc taattccaat gcatttaacg cactacgaac
    12421 atcaccttga ctttgtgtag aaaagtaggt catggcatct tcatcaattt tgggttgata
    12481 tgttttcaaa ccattctctt catcttctat tgcacgagtt aacgcttggc gcacatcttc
    12541 atcatttaaa ggatataact caaaaatttg cgctcttgaa cgaatcgctg gattgatagc
    12601 atgataagga tttgaagttg tagcaccgat caagacgatt ttgccatttt ctaaatgagg
    12661 taataaaaag tcttgtttag ctttatctag tcgatgtatt tcatctaata acaagataac
    12721 ttgaccagac attttagctt cttcaacaac aagttgcata tcttttttag tgttagttac
    12781 agcattcaat tgtctgaatt tatattgcgt actgcccgaa attgctttgg caatacttgt
    12841 tttgcctata ccaggtggac cataaaaaat cattgaagtt aatttttttg tatcaaccat
    12901 tcttctgata atgcctcttg gtccaactaa atgttgttgg gaaatgattt catctatatt
    12961 ttttgggcgc attctcgatg ctaatggttc tgtactcaca tttgtcacac ctttcttata
    13021 ctatcttaac tcaaaaaatg ctaaaatggg aatatcaata ttcgaattaa gtgaggttat
    13081 acaatgaaaa tttctactaa agggagatat ggacttacat tgatgatttc tcttgctaaa
    13141 aaagaggggc aaggatgtat atcattaaag tcaattgctg aagaaaataa tttgagtgat
    13201 ttatatttag aacagcttgt aggtccttta agaaatgcgg ggttaattcg aagtgtacgc
    13261 ggtgctaaag gtggatacca attaagagtg ccagcggaag aaatctcagc aggggatatt
    13321 ataagactgt tggaaggtcc aattacattt gttgaaagta ttgaatcaga accacctgcg
    13381 caaaaacaac tatggattcg catgagagat gcagtgagag atgttttaga taatacaaca
    13441 ttgaaatatt tagcggaata tgtagataca agcgaagatt tagacggata catgttttat
    13501 atttaatcgt ataaatacta tactataaca taaaaacttc atattataat gtttagcgaa
    13561 cctccttagt ggtatataaa tatatacatc caaggaggtt tcactatggc agacgaaagt
    13621 aagttcgatc aatttaaagg aaatgttaaa gaaacagtag gtaacgttac tgataacaaa
    13681 gaattagaaa aagaaggtca gcaagataaa gcgactggta aagcaaaaga agttgttgaa
    13741 aatgctaaaa acaaaataac tgatgcaatt gataaactta aaaaataagt tcaattaaac
    13801 taattaaata taagaggagc gattaatatg agttttatgg ataaagctaa agacgcagta
    13861 gaaaaattta aaaatagcga taatgaacaa gttaaaaatg taaaagataa gataaatgaa
    13921 tatacaggat cgaataacga ag
//
LOCUS       DBNENW010000062        13916 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNENW010000062 DBNENW010000000
VERSION     DBNENW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13916
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.062"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            14..652
                     /locus_tag="AC1LW8_001471"
     CDS             14..652
                     /locus_tag="AC1LW8_001471"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIP4773307.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            655..1578
                     /locus_tag="AC1LW8_001472"
     CDS             655..1578
                     /locus_tag="AC1LW8_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP4773308.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1590..2858
                     /locus_tag="AC1LW8_001473"
     CDS             1590..2858
                     /locus_tag="AC1LW8_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP4773309.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2858..3481
                     /gene="udk"
                     /locus_tag="AC1LW8_001474"
     CDS             2858..3481
                     /gene="udk"
                     /locus_tag="AC1LW8_001474"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIP4773310.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3509..3985
                     /gene="greA"
                     /locus_tag="AC1LW8_001475"
     CDS             3509..3985
                     /gene="greA"
                     /locus_tag="AC1LW8_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIP4773311.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4311..5045
                     /gene="pxpB"
                     /locus_tag="AC1LW8_001476"
     CDS             4311..5045
                     /gene="pxpB"
                     /locus_tag="AC1LW8_001476"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HIP4773312.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            5035..6045
                     /locus_tag="AC1LW8_001477"
     CDS             5035..6045
                     /locus_tag="AC1LW8_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HIP4773313.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQLL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6047..6496
                     /locus_tag="AC1LW8_001478"
     CDS             6047..6496
                     /locus_tag="AC1LW8_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIP4773314.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6510..7871
                     /locus_tag="AC1LW8_001479"
     CDS             6510..7871
                     /locus_tag="AC1LW8_001479"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIP4773315.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALGSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NFVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7871..8623
                     /gene="pxpA"
                     /locus_tag="AC1LW8_001480"
     CDS             7871..8623
                     /gene="pxpA"
                     /locus_tag="AC1LW8_001480"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HIP4773316.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8635..9864
                     /locus_tag="AC1LW8_001481"
     CDS             8635..9864
                     /locus_tag="AC1LW8_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIP4773317.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            complement(10710..10880)
                     /locus_tag="AC1LW8_001482"
     CDS             complement(10710..10880)
                     /locus_tag="AC1LW8_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773318.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(10942..11040)
                     /locus_tag="AC1LW8_001483"
     CDS             complement(10942..11040)
                     /locus_tag="AC1LW8_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773319.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(11059..11958)
                     /gene="sdaAA"
                     /locus_tag="AC1LW8_001484"
     CDS             complement(11059..11958)
                     /gene="sdaAA"
                     /locus_tag="AC1LW8_001484"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIP4773320.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(11972..12652)
                     /gene="sdaAB"
                     /locus_tag="AC1LW8_001485"
     CDS             complement(11972..12652)
                     /gene="sdaAB"
                     /locus_tag="AC1LW8_001485"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIP4773321.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(12655..13698)
                     /locus_tag="AC1LW8_001486"
     CDS             complement(12655..13698)
                     /locus_tag="AC1LW8_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP4773322.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         4776 a         2171 c         2588 g         4381 t
ORIGIN      
        1 ggaagtttaa aacatggatg acctaaataa aaaatattta atagatttac atcaacatca
       61 aaatagttca atcgaagttt tgcgtgaatt tgccgaagta aatgaagtgc caattgtaga
      121 tcgtttaaca ttagatttaa ttaagcaatt aattcgtatg aataatgtta aaaatatttt
      181 agaaattggt acagcaatcg gctatagttc tatgcaattc gcttctatat ctgatgacat
      241 tcatgtcaca acgatagagc gtaatgaaac gatgattcaa tatgctaaac aaaatttagc
      301 tacttatcat tttgaaaacc aggttcgaat tattgaaggt aatgctttag agcaatttga
      361 aaatgtaaat gacaaagttt atgatatgat atttattgat gcagcaaaag cgcaatcaaa
      421 gaaatttttt gaaatatata caccactttt aaagcaccaa ggtctcgtaa ttacagataa
      481 tgttttatat cacggttttg tatcggatat tgggattgtt agatcgagaa atgtaagaca
      541 aatggttaaa aaggttcaag attataatga gtggttaata aagcaaccag gatatacaac
      601 gaatttttta aatatagacg atggattagc gatttcaatt aaaggagaat gactatgaca
      661 gaattacttg tcacacccaa gtcattaagc catatggaaa cacttattga tttaggtgca
      721 gatgcttttg taattggcga gcaaaagttt ggtttaagac ttccagggga attcaataga
      781 caacaaatga cagaggctgt cgcattagct cacaaaaacg ataaaaaagt atatgcggcg
      841 gtgaatggat tatttcataa ttatcattta gatgctgtag aagattatat taatttttta
      901 catgaaatac gagttgacag aattattttc ggggatccag cagtagtaat gtatgtgaag
      961 gcgcaagaga atccaatacc acttcattgg aatgcagaga cgttagtaac taatcatttt
     1021 caatgtaatt actggggtaa aagaggcgca tcaagagctg tcttagcacg tgagctgaat
     1081 ttggatgaaa ttattaatat aaaagaaaat tcaaatgtgg aaattgaggt gcaagttcaa
     1141 ggaatgactt gtatgttcca atcaaagcgt atgctactag gcaattatta tacgttccaa
     1201 gatcgccaaa tgaaaattga acgtcgcaat gatgaacaat ctttattact ttatgatgaa
     1261 gaaagacaaa ataattaccc agtttacgaa gattacaatg gtacgcacat tatgtcacca
     1321 aacgacattt gtttaattga agaattagca ccattttttg aggcgggtat agattcattt
     1381 aaaatcgatg gtattctaca aacggaagaa tatattaatg tggtaacgga acagtatcgt
     1441 caagcgatag atttgtacaa tgaagatcct gaaatctatg aagatgagaa atttatgttg
     1501 atggacccaa ttgaagaaat tcaacctgat catcgaccat ttgacgaagg tttcttatat
     1561 aaacaaacgg tatattaagg aggttaatca tgaagacaat agaagagatt aaatcaactc
     1621 ctaaaacagt tatgaagaaa ccagaattat tagcacctgc tggaaactta gaaaagttaa
     1681 aaatagcagt acattatggc gctgatgccg tatttttagg tggtcaagaa tatggattac
     1741 gttcaaatgc tgataatttc acgatggaag aaatagctga aggtgttgaa tttgcgaacc
     1801 gttacggtgc caaaatttat gttacgacaa atattattgc acatgatgag aatattgaag
     1861 gtctagaatc atatttgcgt aatttggaaa agactggtgc gacaggtatc attgttgcag
     1921 atcctttaat tatcgaaaca tgtaaagaag ttgcgccaaa acttgaaatt catttatcta
     1981 ctcaacaatc actttctaat tacaaagctg tagaatattg gaaagaagaa ggattggatc
     2041 gtgttgtatt agcacgtgag accggcgcga tggaaatgcg tgaaatgaag gaaaaagtag
     2101 atattgaaat cgaagcattt attcatggtg ctatgtgtat cgcctattca ggtagatgta
     2161 cattaagtaa tcatatgact gcaagggatt ccaacagagg cggttgctgt caaagttgcc
     2221 gttgggatta tgaattatta gaagttgatg ataatggtga acttgatgtt ttttataatc
     2281 aaggtgaagt tacaccgttt gcgatgagtc ctaaagattt aaaattaatc gaatcaattc
     2341 ctcaaatgat ggatattggt gtggactcat taaaaattga aggacgtatg aagtcaattc
     2401 attatattgc aacagttgtc tcagtatatc gtaaagtcat tgatgcgtat gcggcagatc
     2461 ctgacaactt taagattaat ccggaatggt taatagagtt agataaatgt gcaaatagag
     2521 acactgcacc agcattcttt gagggaacac ctggttatga agaacagatg tttggtcaac
     2581 aacaatctaa aaaatcacca tttgattttt gtggtttggt attagactat aatgaagata
     2641 caaaaattgc gactattcaa caacgaaata actttaaacc aggtcaagaa atagaattct
     2701 ttggtccaga aattgaaaca tttacacaag tagtagaagc aatttatgat gaagaaggta
     2761 acagcttaga cgcggcgcgc catccattac aaatcgttca aattaaagtg gatcgcccga
     2821 tatatccaaa caacatgatg agaaaggaaa ttggctaatg aaagctacta caatcattgg
     2881 catagctggt ggatctggct caggaaaaac aactgtaact aacgaaatta tgaaaaactt
     2941 agaaggtcat agtgtcgctt tacttgctca agattactat tataaagatc aaaagcactt
     3001 gactttcgac gagcgcctag aaaccaatta tgaccatcca tttgcattcg ataatgattt
     3061 attaattgaa aatcttaaag acttgaaaaa tggtaaagca gtagaagtac cgacatatga
     3121 ttatgctagt catacaagaa gtgacattac cattgatttt aaacctaaag atgttattat
     3181 cgtagaaggt attttcgctt tagaaaataa ggtattacgt gatatgatgg atgttaaaat
     3241 atatgttgat acagatgcag acttgagaat attacgccgt ttaacacgag atactaaaga
     3301 gcgtgggcgt tcaatggact ctgttatcaa tcaatattta agtgttgtta gacctatgca
     3361 tgaccaattt attgaaccga ctaagaaata tgctgatata attattcctg aaggtgggag
     3421 caataaagtt gcaatagata ttatgacaac aaaaattcag tctttagtta gcaagcaata
     3481 gtataattca gtaaaggaag atgacaatat ggaaaatcaa aagcaatatc caatgactca
     3541 agaaggtttt gaaaaattag agcgtgaact tgaagaatta aaaacagtta agcgtcctga
     3601 agttgtagag aaaattaaag ttgcacgttc atttggtgac ttatcagaga actctgagta
     3661 tgatgcagca aaagatgaac aaggattcat cgaacaagat attcaaagaa ttgagcatat
     3721 gttaagaaat gcattaatca ttgaagatac tggagataac aacgttgtta aaattggtaa
     3781 aacagtaacg tttgtagaat taccaggtga tgaagaggaa agttatcaaa tcgttggttc
     3841 agctgaatca gatgcattta atggtaagat ttcaaatgaa tcaccaatgg ctaaagcgtt
     3901 aattggtaaa ggtttagatg atgaagttcg tgttccacta cctaatggtg gcgaaatgaa
     3961 cgtaaaaatt gttaatatcc aataaatgat caaatagatt gtttaattgc gcgattgaag
     4021 tgtgttaatg atacttcggt cgtgcttttt tatattcaaa atcaaaaaat cggacagatg
     4081 aaaagtaaaa attttccatc agaccgaatg atagcagaac ctaaattagt gtaagagtta
     4141 caatattaac tactccattt taaaaaagca aaatatttcc gagaaaacaa attgattatc
     4201 atttgttatt gtatgcgatt tgaattactt attaaacaac ttgtgcttct caatgataca
     4261 atgaaaaggt attcaatact ggtttattgc aatacaaagg gggatttaaa gtggatgtaa
     4321 gatttattaa cgagcagaca attatgattt attttgaaaa taaaatatcc gaagagacat
     4381 atcgtaatgt cacagcaatg gtacggtgga tacgagaaaa agaaatatta gaaattcaag
     4441 atattgtccc ttcgtatcga gcagttctaa tatattttga tgaacaagct ataacatctt
     4501 ctaaattaat tgaaaattta gaacttaaca aatttaatga aaaaaatgtt catgctgtga
     4561 atcaaaccaa tcgtattatt aaaatacctg ttcaatatgg tggtacatat ggaccagata
     4621 ttgaagaagt agcaaagcat aatcgaataa ctgttgagca agttattgaa aaacatacaa
     4681 gtaaacctta tttaatatat atgctaggat ttatgccagg atttccatac ttaggcggac
     4741 ttgatgaaca actacataca cctagacgta atcagccaag gttaaaaatt catgcaggtt
     4801 ctgtaggtat tgcgaataat caaacaggac tatatccatc agattctcct ggcggatggc
     4861 aaattattgg acgcacaccg ttaaaagtat ttagcagcga acgtgaacca atgtcaatgt
     4921 acgaagcagg agaatggata caattttatg caatagatga acaagaattt atacaaatcg
     4981 aaagagatat atcagatggc aactttaatg ttgatgactg ggtggtgatt gaaaatgtca
     5041 attaagattt tacaaccagg tctcttttca acggtacaag atctaggaag aaaaggttat
     5101 gaacatattg gattttcagg cgcaggtgcc atggatcaat ttagttttaa ggttgcgcag
     5161 ttattaatta acaatgatgg tccagcgatt gaatatactt tgattggtcc taccattcaa
     5221 tttaatacgc aaaatacatt tgttataacc ggtggtagtg ttaatgcctc gctaaataat
     5281 aaaactatat caatgaattc tgtcatatta gctgagaaag gtgacatttt aaaaataggt
     5341 gctataacca aaggtgcacg cggttatctt acttttggtc attctatcaa cgtacctcct
     5401 attgctgaaa gttatgcaac acatacccga agcagtatag gaggattcaa aggtagaaag
     5461 ttattagcta atgatgtaat aacagtgaaa ataaataatg actttaaaga aaacattgga
     5521 aagacgattc atttacagga cgatttattg ccagaaaata atattataca cattcttcaa
     5581 ggacctcaat tcgaggcatt ttctgaagag gctagagcga aaattgtaaa tcatccatat
     5641 ttaattactg aacaatcaga ccgtatgggc tatcgtttgg aaggtgacag cgttgcacca
     5701 attaatcaag cagatatcat ttctgaaccg gttgctcttg gtagtgttca agtaccaaat
     5761 gatggtcaac ctattattct gcttaatgat aaacaaacga taggtggtta taccaaaatt
     5821 gcaactgtat gcaaatttga tctgccaaag ttagcacaga tgaaaccaca agatacaatt
     5881 caatttaaat ggatatcatt ccaagaagct gtagataaga atcgggaaca aatgtctctg
     5941 tttaatgaaa ttcttaagag tcatcaaaag actccaattt ttgatacatc tagcttgcga
     6001 catacttcga aaaaattagc aacaatttta aaaggggatt tgtaaaatga atattgaaaa
     6061 aatcgaacaa ataatcaaat tagtgaagga aaatgatgtt aagaaattta aatataaaaa
     6121 ttttgaagat gaaatagaaa ttgacttcac tgactcgaat catttggctg cacacagtaa
     6181 tcaatcaaat caaagtatga acaataatga tttgacagct tcaaaagcga atgataactc
     6241 cgatgtttcg acaaatgatt atcatgacat taaatcacca atggtaggta cattcttttt
     6301 acaagatagt aaagaattaa ctgaaccaat tgtgaatgtc ggtgacaaag ttaacaaggg
     6361 agatattata ggatatgttg aagcgatgaa agtattaaac gaggtaacaa cagatgttgc
     6421 tggagaaatt actgaaatag tagctgatca tggaacaaat gttgaatacg accaagtttt
     6481 ggtacgtatt aagtaaaggg ggtaagtaaa tgcttcgttg tttaattgcg aatagaggtg
     6541 aaattgctgt aaggattata agagcttgca gagaatatgg gattgaaact gtagcagttt
     6601 atgcaaaagg ggacgagcaa agcttgcatg tacatttagc agatcaagct atatgtattg
     6661 gggaagctaa tgctttaggt agttatttaa atattgaccg catcatatct gctgcacaaa
     6721 tcactggtgc taatgcaatt cacccaggat atggcttttt atcagaatca acaaaatttg
     6781 ctcaaaccgt tgaggaacaa ggcatagcgt ttatagggcc tactaaaaaa acgatggaaa
     6841 tgatggggga taaaataaca gcaagacaaa cagttcatca tgcaggtgtt cctgtgattc
     6901 caggatcaaa tggtgctgtg aaccatgtgt ctgaaattga gaagcttgcc aaagacatcg
     6961 gttatccagt tgttatcaaa gctgccagtg gcggtggtgg taaaggtata cgtattgtaa
     7021 agaaagctga agatttagaa aaggcattta aagaagctaa aagtgaagga aaaaaatact
     7081 ttgatgatga tcgtgtttat gttgaggctt ttatacctgt tgcaaaacat gttgaagttc
     7141 aagttatggg agatgggcag gataattttg tacatttagg ggaacgtgat tgttctgtac
     7201 aacgtaaaaa tcaaaaatta atcgaagaat ctccttgtgc agcattaact gaagaaagac
     7261 gacaacaaat atgtaacgat gctgttaaag ttgcacgtgc agcaaattat agaagtgcag
     7321 gtacgattga atttttagta actgataccg cacattactt tattgaaatg aatgctcgaa
     7381 tacaagttga acatactgtt acagagatgc gagcagaacg tgatttggtt gcagcacaat
     7441 tgtatttatt ggaacataat catttacctt tttcacaaag cgatattcaa tttaatggtc
     7501 atgtgataga agcgagaata aatgccgaaa atcctgaaaa gaaatttcaa ccaacaccag
     7561 gtaaagtgac agcgttacat ttacctcaag gctttaatgt gcgagtcgat tctttactat
     7621 atcacggtta tcaagtttca ccatattatg attctttagt agctaaagta attgtaaaat
     7681 ctcatgatag agcttcagca attgataagt tgaaagccac cttagatgaa atggtgatag
     7741 atggattttc tacaactgcc gattttttat atgcagtttt aaattatcca ctatatcgtg
     7801 atggagatgc aaaagatgtt gatattaaat ttttagaaaa acatcaaatt gttaaagggg
     7861 tggaattata atgcgagttg atttgaattg tgatttaggc gaagcatttg gaaattattc
     7921 ctttggtggt gatcatcaaa ttattccgtt aattacaagt gcgaatgttg cttgtggttt
     7981 tcacgctggt gatgaaaatg taatgaatga aacggtaaaa cttgccaaag cacataatgt
     8041 tgcagtaggt gcacatcctg gtttacctga tttgaaaggc tttggcagac gaaatataga
     8101 tatctctaac gacgagattt ataatttgat gatttatcaa ttaggtgcat tacaagggtt
     8161 ttgtcgcatt catcaagtta aaattaatca tgttaaaccg catggtgcgt tgtatcagat
     8221 gggtgcaaaa gacagagaaa tagcaagcgt tatagcacaa gctgtttatg actttgatcc
     8281 atcactagtg ttagtaggat tagcaaattc atatctaatt tcagaagcaa agaatgtcgg
     8341 attaattaca gcttctgaag tgtttgctga tagacgatac gaagatgatg ggcagctcgt
     8401 tagtagaaaa gaaagtgatg ctgtgattac tgatactgac gaagcactta agcaggtttt
     8461 aaagatggtg aaggaaaata aagttatttc aaaaaacaat aaggaagtaa cgttacaagc
     8521 agatacaatt tgtgtgcatg gtgatggaga acatgcatta ttatttgttt cgaaaattag
     8581 agaaatttta atgaaagaag gcattgatat tcaatcctta tagggggcta aaaaatggga
     8641 agaaatttaa agcttaaaaa agaaagtgac tttgaattca caaaaaatca taaaaggtta
     8701 ttattaggtt ctgtattttt gatggcaact tctgcaattg ggccagcatt tttaacgcaa
     8761 acagcagtat ttacatcaca attttttgca agtttcgcat ttgccatatt actgtctatc
     8821 atcattgaca ttggtgcaca aattaatata tggcgcatat tagttgtaac tggtttaaga
     8881 ggtcaagaaa tatcaaataa agttgttcct gggcttggta ctgttatctc aatattgatt
     8941 gcatttggtg gtctagcttt taatattggt aatattgctg gtgccggttt aggtttaaat
     9001 gcaatttttg gattagatgt aaaatggggc gcagctatta ctgcaatctt tgcaatatta
     9061 atctttgtaa gtaaaagtgg ccaaaaaatt atggacgttg tttcaatgat tcttggtatt
     9121 gtgatgattt tagttgtggc atatgtgatg tttgtttcta atccacctta tggtgatgct
     9181 tttgtgcata catttgcgcc agaacatcca atgaaattag tcttgccaat cattacgtta
     9241 gttggtggaa ctgtaggtgg ttatattacc tttgcaggtg cacatcgtat attagactct
     9301 ggcattaaag gtaagcaata tttaccattt gtaaatcaat cagcaattgc tggtatttta
     9361 actacaggta ttatgagaac gttactattc ctagcggtat taggtgttgt tgtaacaggt
     9421 gtgacactaa gttctgaaaa tccaccagcg tcagtttttg aacacgcaat tggaccaatt
     9481 ggaaagaata tttttggtat tgtgttattt gctgcagcta tgtcatcagt aattggctca
     9541 gcatacacaa gcgcaacatt tttaaaaaca cttcataaat cacttaacga aagaagtaat
     9601 ttaattgtga ttgtgtttat cgttatttca acaatgattt tcttatttat tggaaaacca
     9661 atcagccttt taattatagc aggcgcgata aatggatgga ttttacctat tacgttaggt
     9721 gcaattttga ttgcaagtaa aaagaaatca attgttggtg attataaaca cccaaattgg
     9781 atgttcatct ttggtatcgt tgcagtactt gttacaatat tgacaggtat attctcattc
     9841 aaagaagtac ttcagttatt ttaaaagaca ttttgactaa tagcatcact gtaaatgttg
     9901 ttagtattag gcaagatgta ttttgatttc cggtgattca tttataaagc ataattagat
     9961 atatttgttg agagtggtgc atgacaaatg tactgctctt tttgttgtgt atcagtattg
    10021 tgatgtttta atgcgaatat tatatgaatc attatgaaat ttaataaaaa taaaagaaat
    10081 gattatcatt ttttctgata tactgttaaa cggtttggaa tttttatgtt tacactgtat
    10141 tggttgatat aactcaacta ataattgcga acagggtatt tcaaattgga aagtattatg
    10201 aacgtgatac ataatcaaaa ttgtaggctc aagggagtgg gacagaaatg ataaagagcc
    10261 actaatgatt tattatgtag tggttcttac acattagcca cagctaatgt gtacttaaaa
    10321 ataggaatac atgagtaaaa ctcatgcata agaaatacta atttctatag aaaaagtatt
    10381 tctttatcgt cgtcccaccc caacttacat tgtctgtaga aattgggaat ccaatttctc
    10441 tttgttgggg ccccgccaac ttgcacatta ttgtaagctg atttttcgtc agcttctgtg
    10501 ttggggccca caccccaact cgcattgcct gtagaatttc atttcgaaat tctctgtgtt
    10561 ggggcccctg actagaatcg aaaaaagctt gttacaagcg cattttcgtt cagtcaacta
    10621 ctgccaatat aactttgtag agcatattac attgatttac atcgtccctt ttatttattc
    10681 ttttcaaata ctatccccat agctttgatt taacgctttt tctcaataac aaaacgaata
    10741 tagtagaaca tgaaaacgat aatcatgctg agcgataaag atttaaataa tagattgacc
    10801 cacgttccct cagtcgtata tccatatgta atcgttgtgt taatgatgaa tgctataaag
    10861 atgattgata gtcttagcat atcatcactc cttttaagtt attttagata tacggggacg
    10921 cttttgcaat cactattttg attagtatgc attttccata aatctttcaa cttcttcaga
    10981 gataattaag aagcatctat ctggtactaa tgatccagac agatgcttct tttttatcaa
    11041 tattttattg ttatctcatt aattattttt aaccatatct tcagctgtgc caaagatttt
    11101 acgtttaatt gcttcgccag ttggtgtgcc tgctagtcca cccaatccag tttcacgtaa
    11161 tgatgcagga aggttacgac caaccttatc cattgcttca ataacttcat caacagggat
    11221 tctactttca atacctgcta atgctaaatc tgctgaaatt aaagcgttac ccgaaccaat
    11281 tgcatttctc ataacacaag gaatttcaac aagtccggct actggatcac aaactaaacc
    11341 taataaatta cttatcgcta atgccatagc gtgcccggat gcttctggtg atcctccgaa
    11401 tatagctact gctgcagctg cggccattgc agatgctgaa ccaacttcag cttggcagcc
    11461 acctgttgca ccagctacac ttgcattgtt tgctacgaca cgcccaaaca atgctgaagt
    11521 gaataagaaa tcaatcattt gctcttctgt taaatcatgt gttttttcta atttaaaaag
    11581 tgcaccggga atggtacccg aggaaccagc tgttggcgtt gcacaaataa tacccatcgc
    11641 agcattgact tcatttgttg caatggcacc tttgactgcg tcaatcattt catatccaga
    11701 caaagcatga tgtgtttcat tataatcacg tagtttagca gcatcatgac cagtgtagcc
    11761 cgttacactt tcaaccccat cacctgtcgt ccctttgatt actgcgtctc gcatgacatc
    11821 taaattttgt ttcatttgcg ctcgcacttc atcacgtgat ttaccgctta attccatttc
    11881 ttctttaacc atgatatccg caaatgacat attattttct acggcataat ctatagtctc
    11941 tctaattgaa tcaaacatgt ttattccccc tctaatttat ataggaaacg tttacgtcac
    12001 tgtatttctc tttaattgta tttaatgttg attctgagat tgctttattt aatggtatta
    12061 caaccaagca tttatcttca tctatcttaa taaattcatc tttacagtct aatttcatat
    12121 cgttgatatc attgatgaaa tgatttactt gtgctttagt catatttccg tcaacaacta
    12181 aaattggtaa tccatgattt aaatctactt ctagtccatt tatatgaata cctttaattt
    12241 taattgtacc accaccgatt gaaataccga taatttcaat gtagcgacca tcattacgag
    12301 atgatttgat ataagcacag tttggatgtt gaccaatact atcgccttct tcttcgatga
    12361 tatctatttt aataccatca tcagctgcaa tttctaatga agatttaatt cggttatcaa
    12421 atgttgaata tcccattgct ccacccacaa tagcgacatc tgtaccatgt ccttggtgtg
    12481 tttgagcaaa tgattcataa taatgtattt caatattttt aggaatatct cccaatattg
    12541 cgcgtgctga attcccaatc tttactgcac cagccgtatg agaacttgaa gggcccatca
    12601 taactggtcc gataatatcg aaagcacttt gataatcata gctctttgcc ataattaaac
    12661 actctcctta atatgattct ttttgctcgg cattttaaag ttgatattca ttaaattaaa
    12721 cttattaatt agtgttccaa taatataggc taagacaatg ctgacaataa tcactgttgc
    12781 gattgttaca attgatgtca ctgcattatt aaagccaaac aatacgatgg cgcctgcaat
    12841 tggtgttgcc atacctttga cacctattac tagtccgcta aatgtcacga tacatgcgtt
    12901 gacgacccca atcagtgcat ttgtaccata tagttgtact ggatattgcg ctattaaatc
    12961 aatttgcgtc aatggctcaa tacaaactgc aaatgctttt gacggtccac caatgtttaa
    13021 ttttcggaat aaaataaggt taacaaatga gctacctgta catgttagtg ctccaatagc
    13081 cataggaaca cctgtcagtc ctaataaact tgttaatacc attgaactta gcggtgtcat
    13141 acctgtaaca ggaatcacta gtcctaaaat gaccgctaat gcatatggat tgttatcacc
    13201 taccgcagtg acagcactac ctatttgttt taatgttgct agcacaccag gtgtaatgat
    13261 tgatgcaagt ccgaaagcaa ttgctggtgc aaataagatc accacaatta agtccaagcc
    13321 ttctggaact ttcttttcaa tccatttaat taaaaaagct acgccataag ctgcgatgaa
    13381 tgctggtaat aatttaaagt catgtaatac taaaccaaca ataaccgcaa atactggtgc
    13441 aacgcctaag tttaagcacg ttagaatacc tactgcgata ccgcttaaac ttcctgctaa
    13501 atccccaata tcttgtagaa atttaatatc aaatacgcca ccaatagcgt aacttaagaa
    13561 tgcttgtggt agaaatgtcg cacaagctgc accggataat gcttgtagtc cttgtttacc
    13621 gtacggtgca tactttaaaa atagcgtcat gatcactaaa accaagacta atgtgcctac
    13681 acctaacaga atatccattt cccaaaaacc tctctctatg tttattttat tttcagacca
    13741 taaacatcgt acacccttaa gaaaacgttt tcaactttta tctgttatca aatcaaatat
    13801 ttaagtgaaa tatttcatat ttgtgaaaga tttttaaaat ggattgtttc aaaaactact
    13861 tatgttgtca ttaatattta ctaattaact ttttactcta tatttcaaac agttgt
//
LOCUS       DBNENW010000063        13879 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNENW010000063 DBNENW010000000
VERSION     DBNENW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.063"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            245..820
                     /locus_tag="AC1LW8_001487"
     CDS             245..820
                     /locus_tag="AC1LW8_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773323.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(874..2256)
                     /locus_tag="AC1LW8_001488"
     CDS             complement(874..2256)
                     /locus_tag="AC1LW8_001488"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIP4773324.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            2447..3727
                     /locus_tag="AC1LW8_001489"
     CDS             2447..3727
                     /locus_tag="AC1LW8_001489"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIP4773325.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHIFV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEI
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            3733..4794
                     /gene="thrC"
                     /locus_tag="AC1LW8_001490"
     CDS             3733..4794
                     /gene="thrC"
                     /locus_tag="AC1LW8_001490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIP4773326.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            4796..5710
                     /gene="thrB"
                     /locus_tag="AC1LW8_001491"
     CDS             4796..5710
                     /gene="thrB"
                     /locus_tag="AC1LW8_001491"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIP4773327.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIAIQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            5768..6571
                     /locus_tag="AC1LW8_001492"
     CDS             5768..6571
                     /locus_tag="AC1LW8_001492"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP4773328.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(6864..7178)
                     /locus_tag="AC1LW8_001493"
     CDS             complement(6864..7178)
                     /locus_tag="AC1LW8_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773329.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(7153..7257)
                     /locus_tag="AC1LW8_001494"
     CDS             complement(7153..7257)
                     /locus_tag="AC1LW8_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773330.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(7396..8850)
                     /locus_tag="AC1LW8_001495"
     CDS             complement(7396..8850)
                     /locus_tag="AC1LW8_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP4773331.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            9054..10571
                     /locus_tag="AC1LW8_001496"
     CDS             9054..10571
                     /locus_tag="AC1LW8_001496"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HIP4773332.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            10662..10811
                     /gene="rpmG"
                     /locus_tag="AC1LW8_001497"
     CDS             10662..10811
                     /gene="rpmG"
                     /locus_tag="AC1LW8_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP4773333.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            11265..11534
                     /gene="rpsN"
                     /locus_tag="AC1LW8_001498"
     CDS             11265..11534
                     /gene="rpsN"
                     /locus_tag="AC1LW8_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIP4773334.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            11691..12668
                     /gene="guaC"
                     /locus_tag="AC1LW8_001499"
     CDS             11691..12668
                     /gene="guaC"
                     /locus_tag="AC1LW8_001499"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIP4773335.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            12690..13706
                     /locus_tag="AC1LW8_001500"
     CDS             12690..13706
                     /locus_tag="AC1LW8_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIP4773336.1"
                     /translation="MKKFIVTVVAFLSITIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLFTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSVYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
BASE COUNT         5046 a         1945 c         2485 g         4403 t
ORIGIN      
        1 tattagttga ttttagttca tcattgttat aatcaaatta taaactgaca gatattgatg
       61 ttcaatgaat atgacgtgaa agattcgtga attcaagttt atgtcgaatt tatgttataa
      121 cggtcattta aatgacagaa ttaggtcact catagtattt tgaagattga attcattaat
      181 tttaaaatgt ataatgatat ttgtgaaagc gcttgcttag gaggtgtatt tgagagtgaa
      241 tgaaatgaat gctaaagaac aattagtgga caatttaatg aaaacatcat cgcaattatt
      301 taaatttcac ggtgaagttg ccatgcagct tttcttaaat gatgaattaa aattaccttc
      361 tattgttgaa atatgcgtgg aacgtaagcg tttaagtgat attgtgaaag ttattccgca
      421 atcatatgcg ttactataca tagataagca agatcaagca atagctaaag aagatttatc
      481 actttcaaaa attgcaaaag tttatgtgca atatgatgat acaacaataa tgagtatttt
      541 cgtttatgat gtagtaaacg atgaatggat ttttagattg gatccgaata tacgtatacc
      601 taagagtaac atatacttcc atagtttaaa ttgggatgtg gattatatta aaccggagat
      661 tgttctaatg tatgatctaa tgcaacacca tcagtatcat cattattcca attataaacg
      721 agtcatagat gcattaagct actatcaatt ttttatttta aaatttgtag taggtgagca
      781 acgtattaag gatgcaatcc agagaacaat aaataattaa gaaaaagcaa ttcataacgc
      841 agttgaatac atgtgttacg aattgctttt atattagttt ttatcacaca agttttttaa
      901 tgcaaccccg tgatagcaaa actcatatgt agataataca gctttttcag catcatctac
      961 atgaattcca aacatcatcg agatttccga agcaccttgg ttaatcattt ttaagttaat
     1021 ttttgattca gctaaggcat gtgtaatttt atttgcagta ccgatgactt tattcatacc
     1081 ttcgccgaca atcattaata ttgctaaatc atgctcaata cttagctcat caacatcaca
     1141 tttttgacga atttcattta atacttttgt ttctttattt tgaatttgtt ttgaacgcat
     1201 aacgatactg atagtatcaa tacctgaagg catatgatca aatgaaatat tattatcctc
     1261 taagacacct aatatctttc tagtaaaccc gacttgtcta ttcattaaat actttttgat
     1321 attaataacg gtaaaatctt tatcacaact tataccgcta atcacatttt tcgcatttat
     1381 ttctctatca tgcactataa atgtaccttt atcttgaggg cggttcgtat ttttaatcac
     1441 cacagggatg cgatctttat aaagtggttg taaggcttca tcatggaaaa cactaaaacc
     1501 agcataagat aattcacgca tttctcgata agtgatttct tcgattaatt caggatcttt
     1561 gatgatattt ggattagctt tataaatacc agacacatcg gtgaaatttt cataaattgt
     1621 agctctaaca ccacttgata tgatggcgcc agttatatct gatccgccac gtggaaatgt
     1681 aactatatat ccttcatgag atacgccgaa aaatcctggg ataattagtt tctcatcata
     1741 atctcttaat tttttaattt cagagtaagc actatctaat atttgtgctt cttgtgggac
     1801 gtcagtaaca aaaatacccg cttccttcgg tgatatatat tttgttggta taccttgact
     1861 attattatat aaagctatca attgcgcatt aaaatcttca ccacaagaaa gtaatgcatc
     1921 taatagtctc tttggttcat tttttaattg attaatataa tgttccaaag tcacatctat
     1981 cgtccgtaaa atactttcat ccatttgcaa ttcttttaca atatcatcat aacgctgaat
     2041 aatttctctt tttttatcat gataatcaag atgattaatg accttttcat ataatctgat
     2101 taacaaatca gttgttttaa tatcattatc atgtctttta cctggagcag aaacgataac
     2161 aatctttcgc tctggatcag aattaacaat atttaaaacc tttttaattt gagtagcatt
     2221 ggagacggag ctaccaccga atttggaaac tttcatagtg catgccaacc tttctaaaaa
     2281 atcagaaaat tgtattcagt tttgtaagta agaatgatat tatgtattta tattttgaat
     2341 attacgaact ttacaaaaat aaagaataat tctatactat actatcatct agtgtttttc
     2401 aataatacat ttgtttttct caaaagcaca aacggaggta cataaaatga aaaaattaaa
     2461 tatagcatta ttaggattag gtactgtcgg atctggtgtt gttaaaatca tcgaagagaa
     2521 ccgacagcaa attcaagata cattaaataa agatattgtc ataaagcata tttttgttcg
     2581 agataaatct aaaaagagac cgctaaatat tagccaatat catttaactg aagatgttaa
     2641 tgaaatttta aatgatgatt cattagatat tatcgttgaa gtcatgggag gaattgaacc
     2701 aactgtagat tggttaagaa cagcacttaa aaataaaaaa catgttatta ccgcaaataa
     2761 agatttatta gcagtacatc ttaaactttt agaagattta gcagaagaaa atggtgtagc
     2821 tttaaagttt gaagcgagtg tagcaggtgg tattccgatc gtaaatgcca taaataatgg
     2881 tttgaatgcg aataatattt caaaatttat gggaatttta aatggtacct ctaattttat
     2941 tttatctaaa atgactaaag agcaaacgac atttgaggaa gcacttgatg aagcgaaaag
     3001 acttggtttt gctgaagcgg atccaactga tgatgtagaa ggggtagatg cagcgcgtaa
     3061 agttgtcatt acatcatatt tatcatttaa ccaagtcatt aaattaaacg acgttaaacg
     3121 aagaggaatt agtggcgtaa ctttaactga tattaatgta gccgatcaac tggggtataa
     3181 aattaaattg attggtaagg gaatatatga aaatggcaaa gttaatgcat cggtagaacc
     3241 aacgttaatt gataaaaagc atcaattagc agctgtagag gatgaatata acgcgattta
     3301 tgtcattggt gatgccgttg gtgacacgat gttttatgga aaaggagcag gcagtttagc
     3361 aacaggtagt gccgttgtca gtgatttatt gaatgtagca ttattctttg aatcagattt
     3421 acacacattg ccaccacatt ttgaaataaa gacagataaa acacgggaaa tgatggattc
     3481 agatgcagaa attaatatta aagaaaaatc caatttcttt gtagtagtga atcatgtcaa
     3541 aggttcaatt gaaaattttg aaaatgagtt aaaggcaata ttaccatttc accgatcatt
     3601 aagagttgca aattacgata atcaatcata tgccgctgtt atagttggat tggaatcatc
     3661 accggaagaa ttaatcacta agcatggata cgaagttgac aaagtatacc cagtagaagg
     3721 agtttaatta taatgagaag atggcaagga ttagtagaag agtttaaagc acatttacca
     3781 gtaaatgaaa atacaccaaa attaacattg aacgagggaa atacaccact cattcattgt
     3841 gaaaatatgt ctaaaatact aggtatagat ttatatgtga agtatgaagg tgccaatccg
     3901 acaggttcat ttaaagatcg cggtatggta atggctgtga caaaagcaaa agagcaaggt
     3961 aagaaaattg taatatgcgc ttcgactgga aatacatcag cgtctgcagc agcatatgca
     4021 gcgagagcag gtttaaaagc tatcgtcgta ataccagaag gtaaaattgc attaggtaaa
     4081 ttgtcgcaag cagtaatgta tggtgcagaa atcgtttcta ttgaaggaaa ctttgatgaa
     4141 gctttagaaa ttgtaaaaga aattgcaaaa agtggcgaaa tagagcttgt aaactctgtc
     4201 aatccattta gaatcgaagg gcaaaagaca ggctcatttg aaattgtaca acaattagac
     4261 ggtgaagcac ctgatatttt agcgattcct gtaggtaatg caggtaatat tactgcatat
     4321 tggaaaggct ttaaagaata tcatgaagct aaaggttcac aattgccgaa aatgtttggc
     4381 ttccaagctg aaggcgcatc accaattgtt caaaataaag tcattaaaaa tcctgaaacg
     4441 attgcaactg ctattcgaat tggtaatcct gctagttggg ataaggcgac taatgctctt
     4501 aaagaatcaa atggattaat agatagtgtt actgatgatg aaattctaga agcatatcag
     4561 ttaatgacaa ctaaagaagg tgtctttagt gaaccagcga gtaatgcttc tattgcaggt
     4621 ttaattaaat tgcatagaca aggtaaatta cctcaaggta aaaaagtagt tgctatttta
     4681 actggtaatg gattaaaaga tcctgatact gctatttcac tactagataa tccgataaag
     4741 ccattgccaa atgataaaga tagcattatc gattatatta aaggagcttt ataacatgtc
     4801 gaatgttttg gagttaacaa ttcctgcatc aacagccaac cttggagttg gctttgattc
     4861 tataggtatg gctttagata aatttttgca tctgtctgta aaggaaacat cagggacaaa
     4921 atgggaatat attttccatg atgatgcatc taagcaattg cctactgacg aaacaaactt
     4981 tatttatcat gtagcacaac aagttgcttc taaatatagt gttgacttgc ctaatttatg
     5041 tatcgaaatg agaagtgata ttccattggc aagagggtta ggttcgtcag cttctgcttt
     5101 agtaggagct atatatatcg caaattattt tggtgatatc caactgtcta aacatgaggt
     5161 attacaatta gcgactgaaa tcgaaggaca tcctgataat gttgcgccga ccatttatgg
     5221 tggtttaatc gctggatatt ataatgatgt ctcgaaagaa acgtcagttg cacatatcga
     5281 cataccagac gtggatgtga ttgtaacgat accaacttat gaactaaaaa cagaagcatc
     5341 aagacgtgct ttaccacaaa aattaacaca tagtgaagcg gttaaaagta gtgcaattag
     5401 taatacaatg atttgtgcat tagcacagca caattatgaa ttagcaggta aactcatgca
     5461 acaagatggc tttcatgaac cgtatcgtca gcatttaatt gctgaatttg atgaagtgaa
     5521 aacaattgct attcaacata atgcctatgc aactgtaatt agtggtgctg gaccaactat
     5581 tttaatattt agtcgtaaag aaaatagtgg ggaattggtt cgctctttaa atagtcaggt
     5641 agtatcatgc cattctgaat tggtcgatat taatatcagt ggtgttaaag aacgaattgt
     5701 ataccaatag acgctttata ttgtaaaata gtattaaatg cagaatagag aggagattta
     5761 atgcgatatg acaaattata aagttgtcgt tttagatatg gatgacacat tgctaaattc
     5821 agataatgtg atatcagaag aaactgcaaa ttatttaaca gcaattcaag atgaaggtta
     5881 ttatgttgtt ttagcatctg gtagacctac tgaaggtatg attccaactg ctagagattt
     5941 aaaattacct gaacatcata gctatattat tagttataac ggtagtaaaa cgattaacat
     6001 gactaatgaa gaagtagaag taagtaaatc gattggtaag caagatttcg atgaaattgt
     6061 agattattgt cgagatagag gctttttcgt tcttacatat catgatggtc aaattattta
     6121 cgacagcgaa catgagtata tgaatattga agcagaatta acaggtttac cgatgaaacg
     6181 tgttgatgat atcaaagcgt atattcaagg cgatgtaccc aaggtcatgg gtgtagatta
     6241 tgtagcgaat attacagaag ctagaattga tttgaatggt gtgttcaatg ataatgtaga
     6301 tgctacgaca agtaagccat tcttcttaga atttatggcc aaagacgttt caaaaggtaa
     6361 tgcaattaaa gcgttatgtc acaaattggg atattcggtg gatcaagtca ttgcttttgg
     6421 tgatagtatg aatgataaat caatgtttga agtcgcaggt ctagctattg ctatggggaa
     6481 tgcatcagat gaacttaagc aatatgcaaa tgaagttacg ttggatcata atgaaaatgg
     6541 tattccacat gcgctcaaaa aattgttata aattttaaaa taagccttaa cacatgatat
     6601 ttgaataaga tatcttgtgg ttaaggcttt ttatttttgt gaaaatgact tcagttatac
     6661 tatggaggat ttgaaataca tattttagat tagtaatgat atcaaacgaa tagagtaaat
     6721 gtatattttt tgaataaatc aagtattaac tagtcactga aggtagataa ataaatttga
     6781 tcattctgaa taataacgaa tgcattaaaa aatgcagtga caatctaaca ttaattgtta
     6841 aaatcactgc aagcatcttg attttattta gctgtttgtg tccagtcatg cttacctctg
     6901 aaacgtttta catgagcata tatttggtca gcagtataat ctaaatttgt ataaactgta
     6961 atactgaacg ctgattggtt ttcagcaaat gattcttcgg aaaataattc ctttaagtgc
     7021 ataaataaat cattcatttg tttatggttt aaaccgggat attcccttaa agttctcttt
     7081 aataattgtc cgttcttttc ttctaatgct tgaactacaa taacaaatct ttcatcttgc
     7141 tttagtacat catcaaataa atttgtttgt ccaatcattg aatttcaccc ctgaaaagtt
     7201 attgttatct atattataca cgatatgcaa aatatctaaa ataaagaatg gtaacatggt
     7261 aataataaaa tataaacaga atactagtta atacacttta tactagaaaa agatattaaa
     7321 gtatatctgc tttacaccat taaaaagcgg caaatgctat agataatcta aagcatctgc
     7381 cgtatgagaa tttatttatt tttgattgtc ataatcgtgt ggttttaaat taattgtttc
     7441 tagctttaca aattttgttt tgtgaatgat tttatgaata aagtaaatca acgctagaat
     7501 gattaaaggt aaaaagtttt taaaagcatt taaccattga tcttttaaaa tatattcaac
     7561 tgagccacca aatagcaaga ataatagtgt agtgatgaca atgattggtc ctaatggata
     7621 aaaaggtgct ttatatggta ggaccttatt agggtcttga ccttgttttt taatagcttg
     7681 tcgcaatcgt atttgtgacc aaatgcttga tccccaaaca actataatca ttgaaccaat
     7741 aatttcaagt aaattaaaaa cggcatttga attaaagttt gcataaataa taacaataac
     7801 aacgactgca taagtagtta ataatgctct taaaggtaac ttagttgtct tgtttaattt
     7861 acttaaaaat tggggtgctt ttttgtctga acttaaggaa tacaacattc tgcctgttgt
     7921 ataaacacct gaatttgcag cggataataa tgaagttaaa ataaccgcgt tgattactga
     7981 tgctgcaaag gctatgccta ctctatcgaa tacaattgta aatgggcttt gacttattga
     8041 actacttgct cttaataatg atggatctgt gtacggaata attgcaccaa ttactgcaat
     8101 tgataagaca tagaataaaa gaatacgcca aaatacttgt ttaattgcct taggcataga
     8161 ctttttaggg tcatctgatt caccagcagt tactgctact acttctgtac caccaaccga
     8221 aaatccggcg actaataata cgcctaagaa accagagata ccaccaacaa acggtgcttg
     8281 gccttttgta tagttttcaa atccatatgt atgaccacct aagataccga aaatcattaa
     8341 aaagccaaaa ataacgaata cgataattgt taacacttta atcaatgata accaaaactc
     8401 agtttctcca aatgatttta cagaaaaaat gtttaataat agtaaaattg taataaagat
     8461 taagctccaa gtaatggggt ggaaaaattt aaatgtgtcc cagaaataaa gcacatttga
     8521 cgctactatg acatcaacac ttgtaactaa tgaccacaat gcccaataca accatcccat
     8581 ggtaaagcca agagatgagt caataaagcg tgttgaataa gagctgaatg aacctgatac
     8641 tggataaaat gttgccaact ctccaattga tgacattaag aaatatagca tgacaccaat
     8701 aacaagataa gcgagtatag cgcctccagg accagcttga gaaatgatat taccagtagc
     8761 tacaaataga ccagtcccaa ttgcaccacc tatagcaatc atggaaatgt gtcttgagtt
     8821 aagactacgg ttcattttat tatcttccat atttagtctc ccatctattt aaatataccc
     8881 attattgtaa gctttttaag tgtactattc aataactatt tagtactgta aagcgaaaaa
     8941 attaaaattt tctgattttt taatcatctt gagcatgttt aattgtaatt ttgatggggt
     9001 taaattataa tatgtattaa attataatta ttataaattg tggagggatg actatgtcac
     9061 aacaagacaa aaagttaact ggtgtttttg ggcatccagt atcagaccga gaaaatagta
     9121 tgacagcagg gcctagggga cctcttttaa tgcaagatat ttacttttta gagcaaatgt
     9181 ctcaatttga tagagaagta ataccagaac gtcgaatgca tgccaaaggt tctggtgcat
     9241 ttgggacatt tactgtaact aaagatataa caaaatatac gaatgctaaa atattctctg
     9301 aaataggtaa gcaaaccgaa atgtttgccc gtttctctac tgtagcagga gaacgtggtg
     9361 ctgctgatgc ggagcgtgac attcgaggat ttgcgttaaa gttctacact gaagaaggga
     9421 actgggattt agtagggaat aacacaccag tattcttctt tagagatcca aagttatttg
     9481 ttagtttaaa tcgtgcggtg aaacgagatc ctagaacaaa tatgagagat gcacaaaata
     9541 actgggattt ctggacgggt cttccagaag cattgcacca agtaacgatc ttaatgtcag
     9601 atagagggat tcctaaagat ttacgtcata tgcatgggtt cggttctcac acatactcta
     9661 tgtataatga ttctggtgaa cgtgtttggg ttaaattcca ttttagaacg caacaaggta
     9721 ttgaaaactt aactgatgaa gaagctgctg aaattatagc tacagatcgt gattcatctc
     9781 aacgcgattt attcgaagcc attgaaaaag gtgattatcc aaaatggaca atgtatattc
     9841 aagtaatgac tgaggaacaa gctaaaaacc ataaagataa tccatttgat ttaacaaaag
     9901 tatggtatca cgatgagtat cctctaattg aagttggaga gtttgaatta aatagaaatc
     9961 cagataatta ctttatggat gttgaacaag ctgcgtttgc accaactaat attattccag
    10021 gattagattt ttctccagac aaaatgctgc aagggcgttt attctcatat ggcgatgcgc
    10081 aaagatatcg attaggagtt aatcattggc agattcctgt aaaccaacct aaaggtgttg
    10141 gtattgaaaa tatttgtcct tttagtagag atggtcaaat gcgcgtagtt gacaataatc
    10201 aaggtggagg aacacattat tatccaaata accatggtaa atttgattct caacctgaat
    10261 ataaaaagcc accattccca actgatggat atggctatga atataatcaa cgtcaagatg
    10321 atgataatta ttttgaacaa ccaggtaaat tgtttagatt acaatcagag gacgctaaag
    10381 aaagaatttt tacaaataca gcaaatgcaa tggaaggcgt aacggatgat gttaaacgac
    10441 gtcatattcg tcattgttac aaagctgacc cagaatatgg taaaggtgtt gcaaaagcat
    10501 taggtattga tataaattct attgatcttg aaactgaaaa tgatgaaaca tacgaaaact
    10561 ttgaaaaata aatttgatat gtagtttcta tattgcgtag ttgagcagtt tatgatatca
    10621 taataaatca taaagattcc taacaagaga gggtgtttaa cgtgcgcgta aacgtaacat
    10681 tagcatgcac agaatgtggc gatcgtaact atatcactac taaaaataaa cgtaataatc
    10741 ctgagcgtat tgaaatgaaa aaatattgcc caagattaaa caaatatacg ttacatcgtg
    10801 aaactaagta attcttatca ttcaaatacg acgatttgaa aataaagcgg gcttacctat
    10861 tatattgggg agctcgcttt tttatgaaat ttttgtgaag agtgattaat ggattgagtt
    10921 tcatcggtag aacaatatat gattgtatta gttgttactt tattaaaatt tgagaatatt
    10981 tatagaagga aatagattac tgattttata aagtcacttt gttagcgaat gcttgaaaga
    11041 gtatttaata tagtagaatt taaaatttca aagcggaatt taataagtac gaagtaattc
    11101 tgggtatgtt ttataaatgt tcgataatac actttaatct taaatatgat ggtttagaaa
    11161 atgatttaac aaagaaatga aactttactg ttgaattatg tgaggattgt gttattatat
    11221 aaatcgtaat aattacgatt tgataaaaag tgaggtaact atatatggct aagaaatcta
    11281 aaatagcaaa agagagaaaa agagaagagt tagtaaataa atattacgaa ttacgtaaag
    11341 agttaaaagc aaaaggtgat tacgaagcgt taagaaaatt accaagagat tcatcaccta
    11401 cacgtttaac tagaagatgt aaagtaactg gaagacctag aggtgtatta cgtaaatttg
    11461 aaatgtctcg tattgcgttt agagaacatg cgcacaaagg acaaattcca ggtgttaaaa
    11521 aatcaagttg gtaaaatcaa atttcgtact ttagcccatt tacaatatca ataaaacaat
    11581 gtacagtata tacgaatgct ataaactgaa tgttttctca tattaataag gaaacattcg
    11641 gtttttaatt tgcatttaaa aataattagt ttttaaaggg gctatttaaa gtgaaaatat
    11701 ttgattacga agatattcaa ttaataccta ataaatgcat agttgaaagt aggtctgaat
    11761 gtgatacaac tatccaattt ggtccgaaaa aattcaagct acctgtagtt cctgcaaata
    11821 tgcaaacagt tatgaatgag aaattagcga aatggtttgc tgaaaatgat tacttttata
    11881 tcatgcatcg ttttgatgaa gaagcaagaa taccttttat aaaacatatg caaaattcag
    11941 gcttatttgc atctatttca gttggtgtaa agaaagcgga atttgatttt attgaaaagt
    12001 tagctcaaga aaaattaatc cccgaatata ttacaataga tattgcgcat ggtcactcag
    12061 attcagtgat aaacatgatt aaacatataa aaaaccatat acctgatagt tttgttattg
    12121 ctggtaatgt tggcacgcca gaaggtgtta gagaattaga aaatgctggt gctgatgcta
    12181 ccaaagtcgg tataggtcct ggtagagttt gtattacaaa gattaaaaca ggttttggta
    12241 ctggtggttg gcagttagcg gcattaaaca tatgtagtaa agcagctcgt aaacctttga
    12301 ttgccgatgg tggtataaga acgcatggcg acattgctaa atcaattaga tttggtgcat
    12361 caatggtcat gattggttca ttatttgctg cacacgaaga atcacctggt gaaactgtag
    12421 aacttgatgg taaacagtat aaagaatatt ttggtagtgc atctgaattt caaaaaggcg
    12481 aacataaaaa tgtagaaggt aaaaaaatgt ttgtagaaca taagggttca ttaatggata
    12541 ccttaaaaga aatgcaacaa gatttacaaa gctcaatttc atatgccggt ggaaaagact
    12601 tgaaatcatt acgtactgta gattatgtta ttgttagaaa ctctattttc aacggtgata
    12661 gagattaata tttatagtag gtgatgtaaa ttaaaaaatt catagtaact gttgttgcgt
    12721 ttttatcaat tactatcatt gcgccaataa cagaatttaa accattcatt catttacaaa
    12781 atgaagtaag acaatatatt gacattcaca tcaataaaga aacaatttct gcggaaaata
    12841 aattggatac accgaagaaa caacaatttg cctttaataa tatacaaatg aacatgtcga
    12901 aatcagatgt tgagaaaaca ttaaataaac caaaaagagt gacatttaat gaatatggta
    12961 cgaagtggta tacgtattat gatgacgatt acaataattt tataatgata agttacatga
    13021 aagataaagt taatgcgtta tatacaaatc aaaatataat cacttcaaaa tcaaaaatta
    13081 aatacaatac acctaaatcg gttgtaaggc aaagattagg cgaaccagaa acagagattg
    13141 ttaaaggtag agtgcgttac gaacaaaata ataaagaata tgatgttttc cataaaaatc
    13201 acatttatac gacggtattt tatgataagc atcgacgtaa taatgtaaca gctgttttac
    13261 aagtaagtga tgctatggaa aatagattaa aagaacaata tggagcacct tcgaaatcgc
    13321 ttgcagatag ttttgaacta caaaattttg atttagttaa tgctgaaaga aaacaacatc
    13381 aattatttac attgaagtat tctaaacaga attctgaaac tgcacgtaag catagtaaag
    13441 atatggccaa caatcattat tttgatcata caaatttaaa aggtcaatca ccatttgatc
    13501 gattgaaaaa agatggtatt acatttaact cagccggaga gaatttagca tatggtcaag
    13561 ttagtagtgt ctatgcacat caaggattaa tgaattctat tggtcacaga aaaaatattt
    13621 taaatgatac gtttaaaata ttaggtgttg gtgttgattt taatgatgaa aaacaacctt
    13681 tttggacaga aaattatact ggttaatgtt tttggcatga gattaaaggg taatgtttgt
    13741 ctgtaaggaa gtagataaac atgatgacaa cagataaacc aaaagatgcg gatatacttg
    13801 aacgcgtcaa agatatactt aataaaaaag aaagaaaaaa ataataatgg ctcgctcctg
    13861 taaattatta cgggggcga
//
LOCUS       DBNENW010000064        13818 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNENW010000064 DBNENW010000000
VERSION     DBNENW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.064"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            622..1917
                     /gene="purB"
                     /locus_tag="AC1LW8_001501"
     CDS             622..1917
                     /gene="purB"
                     /locus_tag="AC1LW8_001501"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIP4773337.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2026..2328
                     /locus_tag="AC1LW8_001502"
     CDS             2026..2328
                     /locus_tag="AC1LW8_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIP4773338.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2500..3192
                     /locus_tag="AC1LW8_001503"
     CDS             2500..3192
                     /locus_tag="AC1LW8_001503"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HIP4773339.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3189..5381
                     /gene="pcrA"
                     /locus_tag="AC1LW8_001504"
     CDS             3189..5381
                     /gene="pcrA"
                     /locus_tag="AC1LW8_001504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIP4773340.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5385..7388
                     /gene="ligA"
                     /locus_tag="AC1LW8_001505"
     CDS             5385..7388
                     /gene="ligA"
                     /locus_tag="AC1LW8_001505"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIP4773341.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLMAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7401..8600
                     /locus_tag="AC1LW8_001506"
     CDS             7401..8600
                     /locus_tag="AC1LW8_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HIP4773342.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAVDYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8689..10227)
                     /gene="putP"
                     /locus_tag="AC1LW8_001507"
     CDS             complement(8689..10227)
                     /gene="putP"
                     /locus_tag="AC1LW8_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HIP4773343.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10594..10896
                     /gene="gatC"
                     /locus_tag="AC1LW8_001508"
     CDS             10594..10896
                     /gene="gatC"
                     /locus_tag="AC1LW8_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIP4773344.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10898..12355
                     /gene="gatA"
                     /locus_tag="AC1LW8_001509"
     CDS             10898..12355
                     /gene="gatA"
                     /locus_tag="AC1LW8_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIP4773345.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12368..13795
                     /gene="gatB"
                     /locus_tag="AC1LW8_001510"
     CDS             12368..13795
                     /gene="gatB"
                     /locus_tag="AC1LW8_001510"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIP4773346.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         5186 a         2013 c         2573 g         4046 t
ORIGIN      
        1 ataaaaattt actattaaga aaatctctgc gtatttttct ataatcagtt tcgtatttat
       61 acgattctgg agttcaatat taatgtctta tatttaaaat gccataccta tgcaatagaa
      121 catatattct ttttgtgagt agaaagtttg ttatattaga agtgaataat tttaaaacat
      181 attatggaaa tgtaagaagg agtattatga aactaggtaa agtagattgt tgaaaattcc
      241 atacaatagg agaagtaaag tgaataaaat ctggtattac taaagctatt gattgtccta
      301 attgtaataa aaagttggat agtgtagttg aggggagaaa aactaaaatg caataatata
      361 ttcttaatgg atcattataa ataccaatta gattaaagta ttgcgaacat ttacaattag
      421 aaatcagtat taatctgcca tagataattg tattaatgta aaaaataaaa tagaatgaga
      481 atatacggaa attttttata catttataac caattctgtt aaactgtgaa gagtagagtt
      541 aaaaacgaac tttttgttta tttttattaa aaatgttctg tttttgaaaa ttcgagtata
      601 catacgatag gagttaaaaa aatgattgaa cgctattcta gagaagaaat gtctaatatt
      661 tggactgatc aaaatcgcta tgaagcatgg ttagaagtgg aaattttagc atgtgaagca
      721 tggagtgaat taggacatat accgaaagct gacgtacaaa aaattcgtca aaacgcaaag
      781 gtaaatgtcg aacgtgcaca agaaattgaa caagaaacgc gccatgatgt tgtagccttt
      841 actagacaag tttctgaaac gctaggtgaa gaacgtaagt gggtacatta tggtttaact
      901 tctactgatg ttgtagatac agctttaagt ttcgttatta aacaagcaaa tgacattatt
      961 gaaaaagatt tagaaagatt tatcgacgtt ttagctgaaa aagcaaaaaa ttataaatat
     1021 acattgatga tgggacgtac acatggagtg catgcagaac caactacatt tggtgtcaaa
     1081 atggcattgt ggtacacgga aatgcaacgt aacttacaac gtttcaaaca agtaagagaa
     1141 gaaattgaag tagggaaaat gagtggtgca gtaggtactt ttgctaacat tcctcctgaa
     1201 atagaaagtt atgtgtgtaa acatttaggt attggcacag cgcctgtatc aacgcaaaca
     1261 ttgcaacgcg atcgacatgc atactatatt gcgacattag cattaatcgc gacgtcacta
     1321 gaaaaatttg ccgttgaaat tcgtaacctt caaaaaaccg aaacaagaga agttgaagaa
     1381 gcatttgcaa aaggccaaaa aggttcatct gcaatgcctc ataaacgaaa tccaattggt
     1441 tctgaaaata tcacaggtat ttcaagagtc attcgtggtt atattactac agcttatgag
     1501 aatgtgccat tatggcatga aagagatatt tctcattctt ctgctgagcg tattatgtta
     1561 ccagatgtta caatagcact agattatgca ttgaatcgtt tcacaaatat tgtggaccgt
     1621 ttaactgtat ttgaagataa tatgcgtaat aacatagata aaacatttgg tctgattttc
     1681 tcacaacgtg tcttattagc attaattaat aaaggtatgg ttcgtgaaga agcatacgac
     1741 aaagtacaac caaaagcgat gatatcttgg gaaacgaaaa cacctttccg tgaattaatc
     1801 gaacaagatg agtctattac aagtgtttta acaaaagaag aattggatga atgtttcgat
     1861 cctaaacatc atttgaatca agttgacact attttcgaac gcgctggatt agcataaaat
     1921 taaataaagt aacgattcag atttaataaa atataaatcg ctacacttta atagattaat
     1981 gcgttaaaat gacataaaga ataaaattat aggggttgtt tcgatatgca aattgaaaaa
     2041 ttacgaggtg cagcgttaga tgaattgttt gatgcaatac taacgttaga aaatagagaa
     2101 gaatgttacc aatttttcga tgatttgtgt actgtaaatg aaattcaatc actgtctcaa
     2161 agattacaag ttgctaaaat gattaagcaa ggttatacct atgcaacgat tgaacaagaa
     2221 tctggagcat cgactgcaac gatttctaga gtgaagcgtt cattacaatg gggtaatgat
     2281 gcttatacaa tgattttaga tcgtatgaat attgaaacaa atgaataata aatgaacgat
     2341 aaacaatggt tatctatctg cactaataag gtagataatc attgtttttt cacgaaaaaa
     2401 tttacagagt aaaagaactt aaatttcata ttaagtcttt agaactcgac acttaaaaat
     2461 gctataatca tatgtatgtt aaaaaaggag tttcggaaaa tgtatgacat taaaaaatgg
     2521 cgccatattt ttaaattaga cccagctaaa catatttcag atgatgattt agatgcgatt
     2581 tgtatgtctc aaacagatgc aattatgatt ggtggaactg atgacgttac tgaagataat
     2641 gtcattcatt taatgagcag agtaagaaga tacccattgc ctttagtact tgaaatatca
     2701 aacatcgaaa gtgtaatgcc tggttttgat ttttattttg tacctacagt actgaacagt
     2761 acagatgttg tatttcacaa tggtacatta ttagaagcgc ttaaaacata tggacatagt
     2821 atagattttg aggaagtaat atttgaaggg tatgtcgtgt gcaatgctga tagcaaagtg
     2881 gcaaaacata ccaaagcaaa tacagattta acaacagaag atttagaagc atatgcccaa
     2941 atggtcaatc atatgtatcg attaccggtt atgtatatag agtatagtgg catttatggc
     3001 gacgtatcaa aggttcaagc tgtctcagaa catctaacag aaacgcaact tttttatggt
     3061 ggcggtattt cctcagaaca acaagcgaca gagatggcag ctattgcaga tacaattatc
     3121 gtcggtgata ttatttataa agatattaaa aaagctttaa aaacagtaaa aataaaggag
     3181 tctagtaaat gaatgcgtta ttaaatcata tgaatacaga gcaaagtgaa gctgtaaaga
     3241 caacagaagg accattgtta attatggcag gtgctggttc agggaagaca cgtgttttaa
     3301 cacatagaat tgcttattta ttagacgaaa aagatgtctc accatacaat gttttggcta
     3361 ttacttttac aaataaagct gcaagagaaa tgaaagaacg tgttcaaaaa ttagtaggtg
     3421 atcaagcaga agttatttgg atgtcaacat tccactcaat gtgtgttcgt attttacgtc
     3481 gtgatgcaga tcgaattggt atagaacgca attttacgat aattgatcct acagaccaaa
     3541 aatctgttat taaagacgtc ttaaaaaatg aaaatattga tagtaaaaag tttgaacctc
     3601 gtatgtttat cggtgcgatc agtaatttga aaaatgaact taaaacacct gcagatgctc
     3661 aaaaagaagc cacagattat cactcgcaaa tggtagcaac ggtttatagt ggatatcaac
     3721 gccaattgtc acgtaatgaa gcgttagatt ttgatgacct tattatgaca acgattaact
     3781 tatttgagcg tgtaccagaa gttctagaat attatcagaa caaattccaa tatattcatg
     3841 tagatgagta tcaagatact aataaagcac aatacacatt agttaaatta ttagcaagta
     3901 agtttaaaaa cttatgtgtt gtaggtgact cagatcagtc aatttatggt tggcgtggtg
     3961 ctgatattca aaatatctta tcatttgaaa aagactatcc agaagcgaat acaatctttt
     4021 tagagcaaaa ttatcgttcg acgaaaacga ttttaaatgc ggctaacgaa gtgattaaaa
     4081 ataattctga acgtaagcca aaaggactgt ggactgcaaa tacgaatggt gagaaaattc
     4141 attactatga agcaatgacg gaacgtgatg aagcggaatt tgtaatacga gaaattatga
     4201 agcatcaacg taatggtaag aaatatcaag atatggcaat tttatataga acgaatgcac
     4261 aatcacgtgt acttgaggaa acattcatga aatctaatat gccatacaca atggttggtg
     4321 gccaaaagtt ctatgaccgt aaagaaatca aagatttatt aagttatttg cgtataattg
     4381 ccaatagtaa tgatgacatt agtttgcaac gtattattaa tgttccaaaa agaggtgtag
     4441 gtccttcatc tgttgaaaaa gttcaaaact atgcacttca aaacaatatc agtatgtttg
     4501 atgcacttgg agaagctgat tttatcggat tgtcaaaaaa ggtgacacaa gagtgtctta
     4561 atttttacga gttaatccaa agcttgataa aagaacaaga atttttagaa attcatgaaa
     4621 tagttgatga agtattacaa aagtctggct atcgagaaat gcttgaacgt gaaaatacat
     4681 tagaatctcg aagtagatta gaaaacatcg atgaatttat gtcagtacca aaagactatg
     4741 aggaaaatac cccattagaa gaacagtcat taattaactt tttaacggat ttatcgttag
     4801 tagctgatat tgatgaggca gatactgaaa atggcgtaac actaatgacg atgcactcgg
     4861 ctaagggcct tgaatttcca attgtcttta taatggggat ggaagaatct ttattcccac
     4921 atattagagc gattaagagt gaagatgatc atgaaatgca agaagaacgt cgtatttgtt
     4981 atgtagcaat tacaagggct gaagaggtgt tatatatcac tcatgcgaca tcaagaatgt
     5041 tatttggtcg ccctcagtca aatatgccat ccagattttt aaaggaaatt ccagaatcac
     5101 tattagaaaa tcattcaagt ggcaaacgac aaacgataca acctaaggca aaaccttttg
     5161 ctaaacgcgg atttagtcaa cgaacaacgt caacgaaaaa acaagtattg tcatctgatt
     5221 ggaatgtagg tgacaaagtg atgcataaag cctggggaga aggcatggtg agtaatgtaa
     5281 acgagaaaaa tggctcaatc gaactagata ttatctttaa atcacaaggg ccaaaacgtt
     5341 tgttagcgca atttgcacca attgaaaaaa aggaggatta agggatggct gatttatcgt
     5401 ctcgtgtgaa cgagttacat gatttattaa atcaatacag ttatgaatac tatgtagagg
     5461 ataatccatc tgtaccagat agtgaatatg acaaattact tcatgaactg attaaaatag
     5521 aagaggagca tcctgagtat aagactgtag attctccaac agttagagtt ggcggtgaag
     5581 cccaagcctc tttcaataaa gtcaaccatg acacgccaat gttaagttta gggaatgcat
     5641 ttaatgagga tgatttgaga aaattcgacc aacgcatacg tgaacaaatt ggcaacgttg
     5701 aatatatgtg cgaattaaaa attgatggct tagcagtatc attgaaatat gttgatggat
     5761 acttcgttca aggtttaaca cgtggtgatg gaacaacagg tgaagatatt accgaaaatt
     5821 taaaaacaat tcatgcgata cctttgaaaa tgaaagaacc attaaatgta gaagttcgtg
     5881 gtgaagcata tatgccgaga cgttcatttt tacgattaaa tgaagaaaaa gaaaaaaatg
     5941 atgagcagtt atttgcaaat ccaagaaacg ctgctgcggg atcattaaga cagttagatt
     6001 ctaaattaat ggcaaaacga aagctaagcg tatttatata tagtgtcaat gatttcactg
     6061 atttcaatgc gcgttcgcaa agtgaagcat tagatgagtt agataaatta ggttttacaa
     6121 cgaataaaaa tagagcgcgt gtaaataata tcgatggtgt tttagagtat attgaaaaat
     6181 ggacaagcca aagagagtca ttaccttatg atattgatgg gattgttatt aaggttaatg
     6241 atttagatca acaggatgag atgggattca cacaaaaatc tcctagatgg gccattgctt
     6301 ataaatttcc agctgaggaa gtagtaacta aattattaga tattgaatta agtattggac
     6361 gaacaggtgt agtcacacct actgctattt tagaaccagt aaaagtagct ggtacaactg
     6421 tatcaagagc atctttgcac aatgaggatt taattcatga cagagatatt cgaattggtg
     6481 atagtgttgt agtgaaaaaa gcaggtgaca tcatacctga agttgtacgt agtattccag
     6541 aacgtagacc tgaggatgct gtcacatatc atatgccaac ccattgtcca agttgtggac
     6601 atgaattagt acgtattgaa ggcgaagtag cacttcgttg cattaatcca aaatgccaag
     6661 cacaacttgt tgaaggattg attcactttg tatcaagaca agccatgaat attgatggtt
     6721 taggcactaa aattattcaa cagctttatc aaagcgaatt aattaaagat gttgctgata
     6781 ttttctattt aacagaagaa gatttattac ctttagacag aatggggcag aaaaaagttg
     6841 ataatttatt agctgccatt caacaagcta aggacaactc tttagaaaat ttattatttg
     6901 gtctaggtat taggcattta ggtgttaaag cgagccaagt gttagcagaa aaatatgaaa
     6961 cgatagatcg attactaacg gtaactgaag cggaattagt agaaattcat gatataggtg
     7021 ataaagtagc acaatctgta gttacttatt tagaaaatga agatattcgt gctttaattc
     7081 aaaaattaaa agataaacat gttaatatga tttataaagg tatcaaaaca tcagatattg
     7141 aaggacatcc tgaatttagt ggtaaaacga tagtactgac tggtaagcta catcaaatga
     7201 cacgcaatga agcatctaaa tggcttgcat cacaaggtgc taaagttaca agtagcgtta
     7261 ctaaaaatac agatgtcgtt attgctggtg aagatgcagg ttcaaaatta acaaaagcac
     7321 aaagtttagg tattgaaatt tggacagagc aacaatttgt agataagcaa aatgaattaa
     7381 atagttagag gggtatgtcg atgaagcgta cattagtatt attgattaca gctatcttta
     7441 tactcgctgc ttgtggtaac cataaggatg accaggctgg aaaagataat caaaaacata
     7501 acaatagttc aaatcaagta aaagaaattg ctacggataa aaatgtacaa ggtgataact
     7561 atcgtacatt gttaccattt aaagaaagcc aggcaagagg acttttacaa gataacatgg
     7621 caaatagtta taatggcggc gactttgaag atggtttatt gaacttaagt aaagaagtat
     7681 ttccaacaga taaatatttg tatcaagatg gtcaattttt ggacaagaaa acaattaatg
     7741 cctatttaaa tcctaagtat acaaaacgtg aaatcgataa gatgtctgaa aaagataaaa
     7801 aagacaagaa agcgaatgaa aatttaggac ttaatccatc acacgaaggt gaaacagatc
     7861 ctgaaaagat tgcagaaaaa tcaccagcct atttatctaa cattttagag caagattttt
     7921 atggtggtgg agatacaaaa ggtaagaata ttaaaggtat gacgattggt ttagctatga
     7981 atagtgttta ttactataaa aaagaaaaag atggaccgac ttttagtaaa aaactagatg
     8041 atagcgaagt taaaaagcaa ggtaaacaaa tggctagtga gatattatca aggttacgtg
     8101 aaaatgatga tttaaaagat ataccaattc attttgcaat ttataagcaa tcaagtgaag
     8161 attcaatcac accaggtgaa tttatcactc aagcgactgc agaaaagagt caaacaaagc
     8221 ttaatgaatg gcataatatc aatgaaaaat cagctttatt accttcttca acagcagtag
     8281 attatgatga aaatttaaat aataatttca agcaatttaa tgataatttg caatcatatt
     8341 tttctaattt cacacaagca gtagggaaag ttaaatttgt tgataaaaag ccacaacgat
     8401 tagtagtaga tttaccaatc gattactatg gacaagctga aacaattggt attacacagt
     8461 acgttactga acaagcgaat aaatatttcg ataaaatcga taactatgaa attcgtatta
     8521 aagatggtaa ccaaccacgt gctttaatta gtaagacaaa agatgacaaa gaaccgcaag
     8581 ttcatattta cagtaattaa ttgtaattta aagataataa aaaggtacca atgttagtca
     8641 gaattttgat taacattagt acctttttaa tttgttaaga cttatgaatt atttttctct
     8701 aacgatgtcg cggactttgt ttaagtcagt ttcaacaaat gcaccaggtt ttttagtaag
     8761 tttacttaca acatatgtaa cgattacact tacaataaat ccaggaataa tttcatataa
     8821 gccgaatatt tcgttgatat gtgccaatgg tttaatccac gcaatccaaa cgataacgac
     8881 taaggcacct gaaaccattc cacttacagc accggcacgt gtcaaacctt tccagtaaag
     8941 tgcaaatagc acaagtggac tgaacgatgc accaaatccg gcccaagcgt tacctactaa
     9001 gtttagaatt gtgtcgtttg gattccatgc aatcgcgatg gcaacaattg ctacaactaa
     9061 tacagataat cttccaatca taacaaattc tttttggtgc gttttagctt tttcttcacc
     9121 acgaattaat ttataaaagt cttccgttag tgaactagat gttacaagta attgtgaaga
     9181 aatcgtgctc ataattgcag ctagaatcgc agcaagtaag aaaccaccta caagaggatg
     9241 gaagagtact tgactcatca cgatgaataa tgtctcagga tcttctagtt taatatgata
     9301 agcaggtacg aatgcaatac ctgttaaacc aacagccaca gcgcctaata aaccaacagc
     9361 catccagcta atacctaaac gtctagcttt aggtagcatc ttgtgtgatt taatagacat
     9421 aaaccttaca atgatatgag gttgaccgaa ataacctaat ccccatgaaa atagagagat
     9481 aattcctata aatgataacc ctttaaataa atttaaattt gtaggtttca tagctgctac
     9541 atcatgaaac gttccccagc cgtttaaatt catcatagca acaataggga ccataaccat
     9601 cgcaattaac ataatgacac cttggaagaa atctgtaatt gatacagcta aatatccacc
     9661 aaagaaagta tagaaaatga caatgaaagc aactaatatt aaaccgaaat gataatctaa
     9721 tccaaaagca ctttcaaata gtttaccacc agatacgaaa ccagaatgtg tatataatgt
     9781 aaagaatact acgataatca atccagaaat aatctttaac acattatttt tatcgtttaa
     9841 acgattttta aagaaatctg gtaatgtaat tgcatctcca gctaattcgg tataaacacg
     9901 aagtctagga gcaacaacaa agtaatttat ataagcacct aatgttaaac cgattgtaat
     9961 ccacatagct gatagaccag tgctatagac agaaccaggt agccccataa tcatccatcc
    10021 actcatatct gaagctccag ctgataatgc agtaatatac ggtccaatac tacgtccacc
    10081 taacatgtac tcgcttaggt taccagttgc ttgcttgtaa ccgtaaaagc caataacgat
    10141 tagtatcaag aagtagacgg caatcataat ataagtttgc caattggcat ctacttgttg
    10201 acttaatgct gtccccattg taagcatgat aaacactccc tttatttaat ttataattta
    10261 gttacttgaa aacattatac ataaagataa atgtttagaa aagtaggaaa attaaattta
    10321 ttctttttga aatacttgca taccaatata gaaagcgctt tcgtattttt tggatatgag
    10381 caaattttta taattaaaat tgcataatca taatattgaa ttgggttgtc taatattaaa
    10441 agagtgttgt tttcataaaa aatgatttga taaacgaatg ataatgttta tacagtttat
    10501 atgaaattaa agtagcacct cataaatact tagattttta attggaaatt tgatacaatt
    10561 tagtgatgaa tgacttaaag gaggctttta ttaatgacaa aagtaacacg tgaagaagtt
    10621 gagcatatcg cgaatcttgc aagacttcaa atttctcctg aagaaacgga agaaatggcc
    10681 aacacattag aaagcatttt agattttgca aaacaaaatg atagcgctga tacagaaggc
    10741 gttgaaccta catatcacgt tttagattta caaaacgttt tacgtgaaga taaagcaatt
    10801 aaaggtattc cgcaagaatt agctttgaaa aatgccaaag aaacagaaga tggacaattt
    10861 aaagtgccta caatcatgaa tgaggaggac gcgtaagatg agcattcgct acgaatcggt
    10921 tgagaattta ttaactttaa taaaagacaa aaaaatcaaa ccatctgatg ttgttaaaga
    10981 tatatatgat gcaattgaag agactgatcc aacaattaag tcttttctag cgctggataa
    11041 agaaaatgca atcaaaaaag cgcaagaatt ggatgaatta caagcaaaag atcaaatgga
    11101 tggcaaatta tttggtattc caatgggtat aaaagataac attattacaa acggattaga
    11161 aacaacatgt gcaagtaaaa tgttagaagg ttttgtgcca atttacgaat ctactgtaat
    11221 ggaaaaacta cataaagaga atgccgtttt aatcggtaaa ttaaatatgg atgagtttgc
    11281 aatgggtggt tcaacagaaa catcttattt caaaaaaaca gttaacccat ttgaccataa
    11341 agcagtacca ggtggttcat caggtggatc tgcagcagca gttgcagctg gtttagtacc
    11401 atttagctta ggttcagaca caggtggttc aattagacaa ccggctgcat attgtggcgt
    11461 tgtcggtatg aaaccaacat acggtcgtgt atctcgattt ggattagttg cttttgcatc
    11521 ttcattagac caaattggtc cattgactcg aaatgtaaaa gataatgcaa tcgtattaga
    11581 agctatttct ggtgcagatg ttaatgactc tacaagtgca ccagttgatg atgtagactt
    11641 tacatctgaa attggtaaag atattaaagg attaaaagtt gcattaccta aagaatactt
    11701 aggtgaaggt gtagctgatg acgtaaaaga agcagttcaa aacgctgtag aaactttaaa
    11761 atctttaggt gctgtcgttg aggaagtatc attgccaaat actaaatttg gtattccatc
    11821 atattacgtg attgcatcat cagaagcttc gtcaaacctt tctcgttttg acggaattcg
    11881 ttatggttat cattctaaag aagctcattc attagaagaa ttatataaaa tgtcaagatc
    11941 tgaaggtttc ggtaaagaag taaaacgtcg tattttctta ggtacatttg cattaagttc
    12001 aggttactac gatgcttact ataaaaaatc tcaaaaagtt agaacattga ttaaaaatga
    12061 ctttgataaa gtattcgaaa attatgatgt agtagttggt ccaacagcgc ctacaactgc
    12121 gtttaattta ggtgaagaaa ttgatgatcc attaacaatg tatgccaatg atttattaac
    12181 aacaccagta aacttagctg gattacctgg tatttctgtt ccttgtggac aatcaaatgg
    12241 ccgaccaatc ggtttacagt tcattggtaa accattcgat gaaaaaacgt tatatcgtgt
    12301 cgcttatcaa tatgaaacac aatacaattt acatgacgtt tatgaaaaat tataaggagt
    12361 ggaaatcatg cattttgaaa cagttatagg acttgaagtt cacgtagagt taaaaacgga
    12421 ctcaaaaatg ttttctccat caccagcgca ttttggagca gaacctaact caaatacaaa
    12481 tgttatcgac ttagcatatc caggtgtctt accagttgtt aataagcgtg cagtagactg
    12541 ggcaatgcgt gctgcaatgg cactaaatat ggaaatcgca acagaatcta agtttgaccg
    12601 taagaactat ttctatccag ataatccaaa agcatatcaa atttctcaat ttgatcaacc
    12661 aattggtgaa aatggatata tcgatatcga agtcgacggt gaaacaaaac gaatcggtat
    12721 tactcgtctt cacatggaag aagatgctgg taagtcaaca cataaaggtg agtattcatt
    12781 agttgacttg aaccgtcaag gtacaccgct aattgaaatc gtatctgaac cagatattcg
    12841 ttcacctaaa gaagcatatg catatttaga aaaattgcgt tcaattattc aatacactgg
    12901 tgtatcagac gttaagatgg aagagggatc tttacgttgt gatgctaaca tctctttacg
    12961 tccatatggt caagaaaaat ttggtactaa agccgaattg aaaaacttaa actcatttaa
    13021 ctatgtacgt aaaggtttag aatatgaaga aaaacgccaa gaagaagaat tgttaaatgg
    13081 tggagaaatc ggacaagaaa cacgtcgatt tgatgaatct acaggtaaaa caattttaat
    13141 gcgtgttaaa gaaggttctg atgattaccg ttacttccca gagcctgaca ttgtaccttt
    13201 atatattgat gatgcttgga aagagcgtgt tcgtcagaca attcctgaat taccagatga
    13261 acgtaaagct aagtatgtaa atgaattagg tttacctgca tacgatgcac acgtattaac
    13321 attgactaaa gaaatgtcag atttctttga atcaacaatt gaacacggtg cagatgttaa
    13381 attaacatct aactggttaa tgggtggcgt aaacgaatat ttaaataaaa atcaagtaga
    13441 attattagat actaaattaa caccagaaaa tttagcaggt atgattaaac ttatcgaaga
    13501 cggaacaatg agcagtaaaa ttgcgaagaa agtcttccca gagttagcag ctaaaggtgg
    13561 taatgctaaa cagattatgg aagataatgg cttagttcaa atttctgatg aagcaacact
    13621 tctaaaattt gtaaatgaag cattagacaa taacgaacaa tcagttgaag attacaaaaa
    13681 tggtaaaggc aaagctatgg gcttcttagt tggtcaaatt atgaaagcgt ctaaaggtca
    13741 agctaatcca caattagtaa atcaactatt aaaacaagaa ttagataaaa gataatttaa
    13801 atcatcaaac tatgaaga
//
LOCUS       DBNENW010000065        13601 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNENW010000065 DBNENW010000000
VERSION     DBNENW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.065"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            63..2594
                     /gene="secA"
                     /locus_tag="AC1LW8_001511"
     CDS             63..2594
                     /gene="secA"
                     /locus_tag="AC1LW8_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIP4773347.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2636..2740
                     /locus_tag="AC1LW8_001512"
     CDS             2636..2740
                     /locus_tag="AC1LW8_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773348.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            2909..4019
                     /gene="prfB"
                     /locus_tag="AC1LW8_001513"
     CDS             join(2909..2980,2982..4019)
                     /gene="prfB"
                     /locus_tag="AC1LW8_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIP4773349.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            4173..4280
                     /locus_tag="AC1LW8_001514"
     CDS             4173..4280
                     /locus_tag="AC1LW8_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773350.1"
                     /translation="MYLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            4428..5267
                     /locus_tag="AC1LW8_001515"
     CDS             4428..5267
                     /locus_tag="AC1LW8_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG3942 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIP4773351.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKTPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            5441..6091
                     /locus_tag="AC1LW8_001516"
     CDS             5441..6091
                     /locus_tag="AC1LW8_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HIP4773352.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            6088..6324
                     /locus_tag="AC1LW8_001517"
     CDS             6088..6324
                     /locus_tag="AC1LW8_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HIP4773353.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            6593..8578
                     /gene="uvrB"
                     /locus_tag="AC1LW8_001518"
     CDS             6593..8578
                     /gene="uvrB"
                     /locus_tag="AC1LW8_001518"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIP4773354.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIEEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            8586..11432
                     /gene="uvrA"
                     /locus_tag="AC1LW8_001519"
     CDS             8586..11432
                     /gene="uvrA"
                     /locus_tag="AC1LW8_001519"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIP4773355.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            12039..12971
                     /gene="hprK"
                     /locus_tag="AC1LW8_001520"
     CDS             12039..12971
                     /gene="hprK"
                     /locus_tag="AC1LW8_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIP4773356.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            12977..>13601
                     /gene="lgt"
                     /locus_tag="AC1LW8_001521"
     CDS             12977..>13601
                     /gene="lgt"
                     /locus_tag="AC1LW8_001521"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIP4773357.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYVENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEKLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFF"
BASE COUNT         4975 a         1892 c         2704 g         4030 t
ORIGIN      
        1 ctataaatga taagatatat tgttgtaggc caaacagttt tttagctaaa ggagcgaacg
       61 aaatgggatt tttatcaaaa attcttgatg gcaataataa agaaattaaa cagttaggta
      121 aacttgctga taaagtaatc gctttagaag aaaaaacggc aattttaact gatgaagaaa
      181 ttcgtaataa aacgaaacaa ttccaaacag aattagctga cattgataat gtcaaaaagc
      241 aaaatgatta tttagataaa attttaccag aagcatatgc acttgttaga gaaggctcta
      301 aacgtgtatt caatatgaca ccatataaag ttcaaattat gggtggtatt gcaattcata
      361 aaggtgatat cgctgagatg agaacaggtg aaggtaaaac attaacagcg acaatgccaa
      421 catacttaaa tgcattagct ggtagaggtg ttcacgttat tacagtcaat gaatacttat
      481 caagtgttca aagtgaagaa atggctgagt tatataactt cttaggtttg actgtcggat
      541 taaacttaaa cagtaagacg acagaagaaa aacgtgaagc atacgcacaa gacattactt
      601 acagtactaa taatgagcta ggttttgatt acttacgaga taacatggtg aattattctg
      661 aagatagagt aatgcgtcca ttacattttg caatcattga tgaggttgac tcaattttaa
      721 tcgacgaggc acgtacgcca ttaattattt ctggtgaagc tgaaaagtca acgtcacttt
      781 atacacaagc aaatgttttt gcgaaaatgt taaaacagga cgaagattat aaatacgatg
      841 aaaaaacgaa agctgtacat ttaacagaac aaggtgcgga taaagctgaa cgtatgttca
      901 aagttgaaaa cttatatgat gtacaaaatg ttgatgttat tagtcatatc aacacagctt
      961 tacgtgcgca cgttacatta caacgtgacg tagactatat ggttgttgat ggcgaagtat
     1021 taattgtcga tcaatttaca ggacgtacaa tgccaggccg tcgtttctcg gaaggtttac
     1081 accaagctat tgaagcgaag gaaggcgttc aaattcaaaa tgaatctaaa actatggcgt
     1141 ctattacatt ccaaaactat ttcagaatgt acaataaact tgcaggtatg acaggtacag
     1201 ctaaaactga agaagaagaa tttagaaata tttataacat gacagtaact caaattccga
     1261 caaataaacc tgtgcaacgt aacgataagt ctgatttaat ttacattagc caaaaaggta
     1321 aatttgatgc agtagtagaa gatgttgttg aaaaacacaa ggcagggcaa ccagtgctat
     1381 taggtactgt tgcagttgag acttctgaat atatttcaaa tttacttaaa aaacgtggta
     1441 tccgtcatga tgtgttaaat gcgaaaaatc atgaacgtga agctgaaatt gttgcaggcg
     1501 ctggacaaaa aggtgccgtt actattgcca ctaacatggc tggtcgtggt acagatatca
     1561 aattaggtga aggcgtagag gaattaggcg gtttagcagt aataggtaca gaacgacatg
     1621 aatctcgtcg tattgatgac cagttacgtg gtcgttctgg acgtcaaggt gataaagggg
     1681 atagtcgctt ctatttatca ttacaagatg aattaatgat tcgttttggt tctgaacgtt
     1741 tacagaaaat gatgagccga ctaggtttag atgactctac accaattgaa tcaaaaatgg
     1801 tatcaagagc tgtagaatca gcacaaaaac gtgtagaagg taataacttc gacgcgcgta
     1861 aacgtatctt agaatacgat gaagtattac gtaaacaacg tgaaattatc tataacgaaa
     1921 gaaatagtat tattgatgaa gaagacagct ctcaagttgt agatgcaatg ctacgttcaa
     1981 cgttacaacg tagtatcaat tactatatta atacagcaga tgacgagcct gaatatcaac
     2041 cattcatcga ctacattaat gacatcttct tacaagaagg tgacattaca gaggatgata
     2101 tcaaaggtaa agatgctgaa gatattttcg aagtcgtttg ggctaagatt gaagcagcat
     2161 atcaaagtca aaaagatatc ttagaagaac aaatgaatga gtttgagcgt atgattttac
     2221 ttcgttctat tgatagccat tggactgatc atatcgacac aatggatcaa ttacgtcaag
     2281 gtattcactt acgttcttat gcacagcaaa atccattacg tgactatcaa aatgaaggtc
     2341 atgaattatt tgatatcatg atgcaaaata ttgaagaaga tacttgtaaa ttcattttaa
     2401 aatctgtagt acaagttgaa gataatattg aacgtgaaaa aacaacagag tttggtgaag
     2461 cgaagcacgt ttcagctgaa gatggtaaag aaaaagtgaa accgaaacca atcgttaaag
     2521 gcgatcaagt tggtcgtaac gatgattgtc catgtggtag tggtaaaaaa ttcaaaaatt
     2581 gccatggaaa ataaatgata taaaataact ccttccaatt aaacacctat agtttgtgtt
     2641 atgggaggag tctttttatt ttacaagcgt taaatacttt aaaaaatgtg aagaagttgt
     2701 taaacgttgt tatgtactta gttttaaaaa atcggtttag gcatatgtcg atgataaatg
     2761 tactgatttt taacaataaa tgcataaact aattgtcagt gtgcttatat ttcttaacat
     2821 tgttatttaa caaaattatg ttaaaattta gcattataaa agatacaaat caatgacttg
     2881 aattgaaata taaataggag cgaatgctat ggaattatca gaaatcaaac gaaatataga
     2941 taagtataat caagatttaa cacaaattag ggggtctctt tgacttagag aacaaagaaa
     3001 ctaatattca agaatatgaa gaaatgatgg cagaacctaa tttttgggat aaccaaacga
     3061 aagcgcaaga tattatagat aaaaataatg cgttaaaagc aatagttaat ggttataaaa
     3121 cactacaagc agaagtagat gacatggatg ctacttggga tttattacaa gaagaatttg
     3181 atgaagaaat gaaagaagac ttagagcaag aggtcattaa ttttaaggct aaagtggatg
     3241 aatacgaatt gcaattatta ttagatgggc ctcacgatgc caataacgca attctagagt
     3301 tacatcctgg tgcaggtggc acggagtctc aagattgggc taatatgcta tttagaatgt
     3361 atcaacgtta ttgtgagaag aaaggcttta aagttgaaac tgttgattat ctacctgggg
     3421 atgaagcagg gattaaaagt gtaacattgc tcatcaaagg gcataatgct tatggttatt
     3481 taaaagctga aaaaggtgta caccgactag tacgaatttc tccatttgat tcatcaggac
     3541 gtcgtcatac atcatttgca tcatgcgacg ttattccaga ttttaataat gatgaaatag
     3601 agattgaaat caatccggat gatattacag ttgatacatt cagagcttct ggtgcaggtg
     3661 gtcagcatat taacaaaact gaatcggcaa tacgaattac ccaccacccc tcaggtatag
     3721 ttgttaataa ccaaaatgaa cgttctcaaa ttaaaaaccg tgaagcagct atgaaaatgt
     3781 taaagtctaa attatatcaa ttaaaattgg aagagcaggc acgtgaaatg gctgaaattc
     3841 gtggcgaaca aaaagaaatc ggctggggaa gccaaattag atcatatgtt ttccatccat
     3901 actcaatggt gaaagatcat cgtacgaacg aagaaacagg taaggttgat gcagtgatgg
     3961 atggagacat tggaccattt atcgaatcat atttaagaca gacaatgtcg cacgattaat
     4021 atatatttta aaaccgaggc tctaaaaggg cgtcggtttt tggttttttt aaaggtagct
     4081 aaataaattg taaattagat tttggaatat gatttgttta tgaatattta agtacaattc
     4141 ggtagataga gttagaatat attttttgaa agttgtattt gttaaaacaa catatgcagt
     4201 gtgcatttag taatattacc tatggcgatt ttcaaggtat tgaattaatc gttgaaaacg
     4261 ttctcaatta catggtatga atacatttta cactatgata aaaggttgta ttctttttat
     4321 attgttaacc atttgattac atcgttataa caatagcttt tgacaaaatg tattgtgcta
     4381 tagtatttgc atacttaaaa tactaacagc aaaggaatga cagcaagatg aaaaaaactc
     4441 ttacagtgac ggtttcgtca gtgttagctt ttttagcttt aaataatgca gcacatgcac
     4501 aacaacatgg cacacaagta aaaacacctg ttcaacataa ttatgtatca aatgttcaag
     4561 cacaaacgca atcaccgaca acttatacag tagttgctgg cgattcatta tataagattg
     4621 cattagagca tcacttaacg ttgaatcaat tatattcata caatcctggt gtaacacctt
     4681 taatttttcc tggtgacgtg atttcacttg tgcctcaaaa taaagtgaaa caaactaaag
     4741 cggttaaaac accagtaaga aaagcaagcc aagctaaaaa ggtagtaaaa caacctgtac
     4801 aacaagcgtc taaaaaagta gtagttaagc aagcacctaa gcaagcagta gctaagacag
     4861 ttaatgtagc atacaaacct gctcaagtac aaaaatcagt accaactgta cctgttgcac
     4921 ataactacaa taaatcagtt gctaacagag ggaacttata tgcttatgga aactgcacat
     4981 attatgcttt cgatcgtcgt gcacaattag gtagaagtat aggaagttta tggggcaatg
     5041 caaataactg gaattacgca gcaaaagttg caggatttaa agtagataaa acaccagaag
     5101 ttggcgctat tttccaaaca gctgctggcc catatggaca tgttggtgtt gttgaatctg
     5161 taaaccctaa tggaacaatt actgtttctg aaatgaacta tgctggattt aatgttaaat
     5221 cttcaagaac aattttaaat ccaggaaaat ataattacat ccactaagta atatatcaag
     5281 acaagactat cctcttagcc tgtttaagta acaggttgag aggatttttt ggtatcattt
     5341 aatcagagtt atataaagaa gatatttaaa tgatatttaa ttgattgata tgtaaaaaga
     5401 aagtataatt tatgattaat taatggagga ggtaattgaa atgggtgtac atcaatattt
     5461 taaaagatta tcagatatgg aaagacttat aagattacct ggaaaattta aatattttga
     5521 acacaatgtt gcagcacact cctttaaagt aactaaaatt gctcaatatc tagcaacagt
     5581 tgaagaatat catggacgaa agattaattg gaaaagctta tatgaaaaag cattaaatca
     5641 tgatttcgcc gaagtgttta ctggtgatat aaaaacacct gttaaatatg cgagtagtga
     5701 attaaaaaaa ttattttcgc aagttgaaga agaaatggta gagaccttta ttgaagaaga
     5761 aattccatta caatatagag atgtttataa gcaacgactg caagaaggta aagatgattc
     5821 attagaaggc caaatacttt cagttgctga taaaattgat ttgctttatg aaacatttgg
     5881 agaaatacaa aaacgtaatc ccgaagaatt atttttcgaa atttatgaaa tgagtctaga
     5941 aacaattatt caatttgacc atttagcatc tgtacaagat tttattaata atatcattcc
     6001 agaaatgttg actgaaaact ttatacctag aacagaatta agagaaacaa ccatgaacat
     6061 tttaaataaa agaaaagagg aaaatgaatg atatggtatt ttagcgcagc attctttcca
     6121 tgtgtcttgg tagtattatt tagtgtaata acaagaagta aatgggtcgg tactattctg
     6181 acattaattt taattggtgc ctcaatctat aaagagtatt tccataacga gtggattatt
     6241 tttattgatg tagtgtcatt attagctggt tatttaatta tagatcaact cgaatttcat
     6301 aaacatcaag atgaagatcg ctaagattaa ctttaaaata atgtttcaaa caaatttgtt
     6361 gaaacaaaat gatgattaat ataatgtgta tttacatact aaaaataaca agataaacga
     6421 tttgatttaa ggcaagcata gttagcacaa ctatgtttgt ttttctttgt tcgacatttt
     6481 tacgaacaaa cgtttgcttt ttgtgtgact actttgctaa aatatgtaat gagaaagcaa
     6541 agagttgagc ggaaatataa ataatacgtt gaaagaggag gcatatgtga caatggttga
     6601 acattatcct tttaaaatac attctgattt tgagcctcaa ggtgaccaac cgcaagcaat
     6661 tgaagaaatc gtggaaggta ttaaagcggg gaaaagacat caaactttat taggtgctac
     6721 tggcacaggg aaaacattta cgatgagtaa tgttattaaa gaagttggga aaccaacgtt
     6781 aattatcgcg cataacaaaa cattagcagg acaattatat agtgagttta aagaattttt
     6841 tcctgaaaac agggtggaat actttgtaag ttactatgat tattatcaac cagaggcata
     6901 cgtaccgtct actgacactt ttattgaaaa agatgcctca atcaatgatg aaattgatca
     6961 actacgacat tctgctacaa gtgcattatt tgaacgcgat gatgtaatta ttattgctag
     7021 tgtaagttgt atatatggtt taggtaatcc tgaagaatat aaagatttag tagtaagtgt
     7081 tcgagttggt atggaaatgg atagaagtga attacttaga aaacttgtag atgtgcaata
     7141 tacacgaaat gacatcgatt tccaacgagg aacgtttcga gtgcgtggtg atgtagttga
     7201 aatattccca gcctctaaag aagaactttg tataagggtt gagtttttcg gcgatgagat
     7261 tgaccgtatc cgagaagtta actacctaac aggtgaagtg ttgaaagaaa gagaacattt
     7321 tgcgatattc ccagcttctc acttcgtaac acgtgaagaa aagttgaaag ttgcgattga
     7381 acgtattgaa aaagaattgg aagaacgatt gaaagaatta cgagatgaga ataaattact
     7441 agaagcgcaa aggttagaac agcgtaccaa ctatgattta gaaatgatgc gagagatggg
     7501 attctgttca ggaattgaaa actattccgt acatttaact ttgcggccac tgggttcgac
     7561 accatatact ttattggatt actttggcga tgattggtta gtaatgattg atgaatcaca
     7621 tgtgacatta ccgcaagttc gaggcatgta taacggagac agagcgcgta aacaagtttt
     7681 ggtggatcat ggatttagat taccgagtgc attagataac cgtccactta aatttgaaga
     7741 atttgaagaa aagacaaaac aacttgtgta tgtatctgca acgcctggac catacgaaat
     7801 tgaacatacg gataagatgg ttgaacaaat tattcgtcct actggtttac tggatcctaa
     7861 gattgaggtt agacctactg aaaatcaaat tgacgattta ttaagtgaaa ttcaaacaag
     7921 agttgagcgt aatgaacgcg tacttgttac aacgctcact aaaaagatga gtgaagattt
     7981 aaccacatac atgaaagaag cgggtattaa agttaattat ctgcattcag aaatcaagac
     8041 attagaacga attgaaataa ttagagactt acgaatgggt acatatgatg ttatcgtagg
     8101 tattaattta ttaagagagg gtattgatat accagaagtt tctctagttg tcatattaga
     8161 tgcagataaa gaagggtttt tacgttctaa ccgctcatta attcaaacaa taggtagagc
     8221 tgcgcgtaac gataaaggtg aagtcattat gtatgccgat aaaatgactg attcgatgaa
     8281 gtatgcaatt gatgagacac aacgtcgtcg agaaatacag atgaaacata atgaaaaaca
     8341 tggtattaca cctaaaacaa ttaataaaaa aatacatgat ttaattagtg ctactgttga
     8401 aaatgacgaa aataatgaca aagcacaaac tgtgatacct aagaagatga cgaaaaaaga
     8461 acgtcaaaag acaatcgaca atatagaaaa agaaatgaaa caagcagcga aagatttaga
     8521 tttcgagaaa gctacagaat taagagatat gttatttgaa ttaaaagcag aagggtgaca
     8581 agtaaatgaa agaaccatcc atagtagtaa aaggtgctcg tgcgcataac ttgaaagata
     8641 ttgatatcga actacctaaa aataaattaa ttgttatgac aggtttatct gggtcaggta
     8701 aatcgtcatt agcattcgat actatatatg ctgaaggaca acgacgttat gttgaatcat
     8761 taagtgccta tgcgcgtcaa tttttaggcc aaatggacaa accagatgtt gatacaattg
     8821 aaggattatc gccagcaatt tcaatagatc aaaaaacaac aagtaaaaat ccaagatcaa
     8881 ctgtagcaac agtaacagaa atatatgatt atatacgttt gttatatgca cgtgttggta
     8941 aaccttactg tccaaatcac aatatagaaa ttgaatcgca aacagtacaa caaatggttg
     9001 accgcattat ggaattagag gcacgtacaa agattcaatt attagcacct gtcatcgctc
     9061 atcgtaaagg tagtcatgaa aagctaatcg aagatattgg taaaaaaggt tatgtacgtt
     9121 taagaatcga tggcgaaatt gttgatgtaa atgatgtacc tactttagat aagaacaaga
     9181 atcatacaat agaagttgtt gtagaccgat tagttgttaa agatggaatt gaaacacgac
     9241 tagctgactc tatagaaact gccttagagc tttcagaagg acaattaaca gtcgatgtca
     9301 ttgacgggga agaccttaag ttttcagaaa gccatgcttg tcctatatgt ggattttcaa
     9361 tcggagagtt agaaccaaga atgtttagct ttaacagtcc ttttggtgct tgtccgacat
     9421 gtgatggctt aggccaaaag ttaacagtcg atgtagactt ggttgttccc gacaaagata
     9481 agacgctaaa cgaaggtgca atagaacctt ggataccgac gagttctgat ttttatccaa
     9541 cattgttaaa acgtgtttgt gaagtttata aaatcaatat ggataaacct tttaaaaagt
     9601 taacagaacg tcaacgtgat attttattgt atggttctgg tgacaaagaa attgaattta
     9661 catttacaca acgtcaaggt ggtactagaa aacgaacaat ggttttcgag ggtgtagttc
     9721 ctaatataag tagacgattc catgaatctc cttcagaata tacacgtgaa atgatgagta
     9781 aatatatgac tgaactacct tgcgaaactt gtcatggaaa gcgattgagt cgtgaagcgt
     9841 tatctgttta tgtaggtggt ttaaatattg gtgaagtagt cgaatattca atcagtcaag
     9901 cgctgaacta ttataaaaac attgatttgt cagaacaaga tcaagcgatt gcaaatcaaa
     9961 tattgaaaga aattatttcc cgactcactt ttttaaataa tgtgggactt gaatatttaa
    10021 cgttaaacag agcttcaggt acactttcag gtggtgaagc acaacgtatt cgattagcaa
    10081 cgcaaattgg gtcgcgtttg actggtgtct tatatgtatt agatgagcca tcaattggac
    10141 tgcatcaaag agataatgat cgattaatta atacacttaa agaaatgaga gatttaggaa
    10201 atactttaat tgtagttgaa cacgatgatg atacaatgcg tgcggctgat tacttagtgg
    10261 atataggtcc tggtgctggt gaacatggag ggcagattgt gtctagtggt actcctcaaa
    10321 aggtaatgaa agataaaaaa tcattaacag gacaatactt gagtggtaag aaacgtattg
    10381 aagtacctga atatcgcaga ccggcttcag atcgtaaaat ttctatacgt ggagctagaa
    10441 gcaacaatct taaaggggtt gatgtggaca taccactatc aatcatgacg gttgttacag
    10501 gtgtatcagg ttctggtaaa agctcattag taaatgaagt attatacaaa tcattagctc
    10561 aaaaaattaa taaatctaaa gtaaagccag gattgtacga taagattgaa ggtattgatc
    10621 aacttgataa aattattgat attgatcaat caccaatagg tagaacgcca cgctctaatc
    10681 cagcaacata tactggtgtg tttgatgata tacgtgatgt gtttgcgcaa acaaatgaag
    10741 ctaaaattcg aggatatcaa aaagggcgtt ttagttttaa tgtaaaaggt ggacgctgtg
    10801 aagcttgtaa aggtgacggt attattaaaa ttgaaatgca ttttttacct gatgtttatg
    10861 ttccttgtga agtgtgtgat ggtaaacgat ataatcgtga gacactagag gttacttaca
    10921 aaggtaaaaa tattgctgac attttagaaa tgactgttga agaagcaaca caattttttg
    10981 aaaatattcc taagattaag cgcaagttac aaacactagt tgatgttggt cttggatacg
    11041 tcacattagg tcaacaagct acaacgttat caggtggtga ggctcaacgt gtgaaacttg
    11101 catctgaact tcataaacgt tcaactggta aatctattta tatcctagat gaaccgacaa
    11161 cagggttaca tgttgacgat attagtagat tattaaaagt attaaaccga ttagttgaaa
    11221 atggtgatac tgttgtaatt attgaacata acctagatgt tatcaaaaca gcagactata
    11281 ttatagactt aggtcctgaa ggtggtagtg gcggtggtac tattgttgcg actggcacac
    11341 ccgaagatat tgctcagaca aagtcatcat atacaggaaa gtatttaaaa gaagtacttg
    11401 aacgagataa acaaaatact gaagataaat aagattaaaa gaagtgaagg atgttataaa
    11461 tttatccttc gcttcttttt attaatttag taatgaatag tagaaagaaa agatgcgtaa
    11521 aaagaattat gttaagatag ggtcaatcta gagtagttaa acataaatcg aactgggagt
    11581 gggacagaaa tgataaagaa tcactaatga tttattatgt agtggttctt tgtcattagc
    11641 cacagctatt gtgtacttaa aaataggaat gcatgagtgc aactcatgca taagaaatac
    11701 taatttctaa agaaaaagta tttctttatg ttggggcccc gccaacttgc attgtttgta
    11761 gaatttcttt tcgaaattct ttatgttggg gccccgccaa ctaattccaa tatatcattg
    11821 tagagcttag gtcattgatt tttggctcgg acttttatgg cgatatgaac catgtaaatt
    11881 aagcaagcaa taaattaatg attgatattg acttgtaaaa taataacaat aatgaacaat
    11941 taatatttat tttagctttt caatgtagat tggtgttata tttttgatat gataagaaga
    12001 gatgtaagag tagggataaa tacaattgag gtgaacccat gttaacgaca gaaaaactag
    12061 ttgaaacatt aaagttagat ttaatcgctg gtgaagaagg actatcgaag ccaattaaaa
    12121 atgctgatat atcaagaccg ggcttagaga tggcaggtta tttttcacat tatgcgtcag
    12181 atagaataca actattagga acaacggaac tatcgtttta caatttatta ccagataagg
    12241 atcgcgcagg tcgtatgcgt aaactatgca gaccagaaac gcctgcaatt attgtgacgc
    12301 gtggattgca gccaccagaa gaattagttg aagctgcaaa agaattaaat accccactta
    12361 tagttgctaa agatgcgact acaagtttaa tgagtcgctt aacaacgttt ttagagcatg
    12421 cacttgcaaa gacgacatct ttacatggtg ttttagtaga tgtttacggt gttggtgtac
    12481 taattaccgg tgattcagga ataggtaaaa gtgagactgc gttggaatta gttaaacgtg
    12541 ggcatagatt agtagcagat gataatgtag aaatacgtca aattaataaa gatgaactaa
    12601 tagggaaacc accaaagtta atagaacatc tattagaaat acgtggacta ggtattatca
    12661 atgttatgac tttatttggc gcgggttcaa tattaactga aaaacgaatt agattaaata
    12721 ttaatttgga aaactggaac aagcaaaagt tatatgaccg cgtaggtctt aatgaagaga
    12781 cgctaagtat tttagatact gaaatcacta aaaaaacaat acctgtaaga cctggtagaa
    12841 atgttgcggt aattattgag gtcgctgcaa tgaactatcg attaaatatc atgggcatta
    12901 acactgccga agaatttagt gaaagattaa atgaagaaat tatcaagaac agtcataaga
    12961 gtgaggagta ggttgaatgg gtattgtatt taactatata gatcctgtgg catttaactt
    13021 aggaccactg agtgtacgat ggtatggaat tatcattgct gtcggaatat tacttggtta
    13081 ctttgttgca caacgtgcac tagttaaagc aggattacat aaagatactt tagtagatat
    13141 tattttttat agtgcactat ttggatttat cgcggcacga atctattttg tgattttcca
    13201 atggccatat tacgtggaaa atccaagtga aattattaaa atatggcatg gtggaatagc
    13261 aatacatggt ggtttaatag gtggctttat tgctggtgtt attgtatgta aagtgaaaaa
    13321 tttaaaccca tttcaaattg gtgatatcgt tgcgccaagt ataattttag cgcaaggaat
    13381 tggacgctgg ggtaacttta tgaatcacga ggcacatggt ggacctgtgt cacgcgcttt
    13441 tttagaaaaa ttacatttgc caaattttat aatagaaaat atgtatatta acggccaata
    13501 ttatcatcca acattcttat atgaatccat ttgggatgtc gctggattta ttatcttagt
    13561 taatattcgt aaacatttaa aattaggaga aacattcttt t
//
LOCUS       DBNENW010000066        13014 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNENW010000066 DBNENW010000000
VERSION     DBNENW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.066"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(213..875)
                     /locus_tag="AC1LW8_001522"
     CDS             complement(213..875)
                     /locus_tag="AC1LW8_001522"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4773358.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1267..1326
                     /locus_tag="AC1LW8_001523"
     CDS             1267..1326
                     /locus_tag="AC1LW8_001523"
                     /inference="COORDINATES: protein motif:HMM:NF019858.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HIP4773359.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            1384..2115
                     /gene="erm(A)"
                     /locus_tag="AC1LW8_001524"
     CDS             1384..2115
                     /gene="erm(A)"
                     /locus_tag="AC1LW8_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HIP4773360.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2241..3023)
                     /locus_tag="AC1LW8_001525"
     CDS             complement(2241..3023)
                     /locus_tag="AC1LW8_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HIP4773361.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3174..3551)
                     /locus_tag="AC1LW8_001526"
     CDS             complement(3174..3551)
                     /locus_tag="AC1LW8_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HIP4773362.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(3558..5450)
                     /locus_tag="AC1LW8_001527"
     CDS             complement(3558..5450)
                     /locus_tag="AC1LW8_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP4773363.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(5447..6532)
                     /locus_tag="AC1LW8_001528"
     CDS             complement(5447..6532)
                     /locus_tag="AC1LW8_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP4773364.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            6665..6961
                     /locus_tag="AC1LW8_001529"
                     /pseudo
     CDS             6665..6961
                     /locus_tag="AC1LW8_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
     gene            7702..8088
                     /locus_tag="AC1LW8_001530"
     CDS             7702..8088
                     /locus_tag="AC1LW8_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIP4773365.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            8081..8377
                     /locus_tag="AC1LW8_001531"
                     /pseudo
     CDS             8081..8377
                     /locus_tag="AC1LW8_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     regulatory      8435..8538
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            8627..9652
                     /locus_tag="AC1LW8_001532"
     CDS             8627..9652
                     /locus_tag="AC1LW8_001532"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIP4773366.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            9645..10340
                     /locus_tag="AC1LW8_001533"
     CDS             9645..10340
                     /locus_tag="AC1LW8_001533"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIP4773367.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            10358..11179
                     /locus_tag="AC1LW8_001534"
     CDS             10358..11179
                     /locus_tag="AC1LW8_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIP4773368.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMALYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            11533..11727
                     /locus_tag="AC1LW8_001535"
     CDS             11533..11727
                     /locus_tag="AC1LW8_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIP4773369.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(11960..12811)
                     /locus_tag="AC1LW8_001536"
     CDS             complement(11960..12811)
                     /locus_tag="AC1LW8_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIP4773370.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         4386 a         2175 c         2031 g         4422 t
ORIGIN      
        1 acaaaagaat catttaaatc aatactttca ctttgaatac atgtatttga agtggaaggt
       61 acttatttca aaaatagtaa aacctgtatc ttaaattact taatagtaac ataagataca
      121 ggctgatttt ttattcattg ttatttatac taaagcaccc gatagctctg aaaacaatca
      181 caaattcaac ttttcaaagc cacagcttta agttattttg tccagacaac ccccattgcc
      241 caacccattt tatggaattg gcatccaggc aacaactttt catataaatc gtaataattt
      301 tgttcagata ggtacttatc tgaagctaaa tgctcaagcc atgatttaga tgtgttgtga
      361 ttataaattc taatcgcatt ttttattcca aatttgaaac aatgcggtaa aaactcttgg
      421 attgccccta atttataaac ataggtaggc ggtgtctcga cttcagatac attatcaaga
      481 ataactattc ttccttcctc attcaacagt tccttcattt gctgtattac gctggctata
      541 tcatccaaat gatgaaaggt tgttcggctt acaataaaat caaacttctc attaaaatta
      601 agttgttctg cattcatatt cagatagacc gtatttgtta gttgacgttt agatttggca
      661 agatcgagca tttgattaga aatatcaatc cctaccactt catcataata acttgctaat
      721 ttctccacta acaaacccga gccacatccg atatctaatg ctctgccttt ctttggagac
      781 atattagaca caaagaatga ataatcattc aaaagctcat tcacgaaatc gtaatcttct
      841 gcaaccttat caaactgtga ttctattgta ttcaaaaaga tcccccattc ctactttatc
      901 gacattcttt cattacttac cactttagat gttttttcgt tggggataaa acttcccttt
      961 agacaatttt atccaaagac aatacaacag tgcaacttta ttaaagtcac tgtcctttat
     1021 cgcagccttt actttttagt aaagacagtg gcttctctta tcaagtttca aaacatatta
     1081 ttttgaagaa aacgtccatc tgaagtgtca agtgcaaaat tacatataaa ggtttattct
     1141 aaaatgaaaa gatgatacaa tcatattcag ttacataagg aggtttcaat tatgtgcacc
     1201 agtatcgcag tagtagaaat tactttatct cattcataat gaaaaaaatg gaaaggagat
     1261 aaaagtatgg gtactttttc tatatttgtt attaataaag ttcgttatca accaaatcaa
     1321 aattaattgg ttataatgaa cgcttaatgt cagttcatta taaccagtaa ggagaaggtt
     1381 ataatgaacc agaaaaaccc taaagacacg caaaatttta ttacttctaa aaagcatgta
     1441 aaagaaatat tgaatcacac gaatatcagt aaacaagaca acgtaataga aatcggatca
     1501 ggaaaaggac attttaccaa agagctagtc aaaatgagtc gatcagttac tgctatagaa
     1561 attgatggag gcttatgtca agtgactaaa gaagcggtaa acccctctga gaatataaaa
     1621 gtgattcaaa cggatattct aaaattttcc ttcccaaaac atataaacta taagatatat
     1681 ggtaatattc cttataacat cagtacggat attgtcaaaa gaattacctt tgaaagtcag
     1741 gctaaatata gctatcttat cgttgagaag ggatttgcga aaagattgca aaatctgcaa
     1801 cgagctttgg gtttactatt aatggtggag atggatataa aaatgctcaa aaaagtacca
     1861 ccactatatt ttcatcctaa gccaagtgta gactctgtat tgattgttct tgagcgacat
     1921 caaccattga tttcaaagaa ggactacaaa aagtatcgat cttttgttta taagtgggta
     1981 aaccgtgaat atcgtgttct tttcactaaa aaccaattcc gacaggcttt gaagcatgca
     2041 aatgtcacta atattaataa actatcgaag gaacaatttc tttctatttt caatagttac
     2101 aaattgtttc actaaattaa agtaataaag cgttctctaa tttcacaaga ggacgcttta
     2161 ttcttcccaa aaattgttca atatttatca ataaatcagt agttttaaaa gtaagcacct
     2221 gttattgcaa taaaattagc ctaattgaga gaagtttcta tagaattttt catatactta
     2281 acgagtgctt tcacctttga atatagtcct tcccacttat catcacactc tccccgatag
     2341 ccttttctag ctatatccag taaagttaca tgctctttag gtaaaagagg tatagcccat
     2401 tctgcagcga catctttcga ggtaatttca ccagtagtca ctgtttgcca cattcgagct
     2461 agggttaaaa ttacattacg ctcatcacct tttatcccct caattagttc tggcaaagaa
     2521 tccttaattg ctcttcgaat atctgtcaaa ggtacggaga caagtatact tgaagaatca
     2581 ggaccaaata gagaaatact attctttctt gcttgtgcta aaacaatagc caaatcagga
     2641 tcatagcttg gttcctgaat ttgtccattc tcaaattcac ccctgagcca ctcaccgtat
     2701 ataaattctc tttttggagg atattgccaa gggacaactt cactcctatt tataaccgta
     2761 acttcaagtg gtctaacaga atccgtattt ccaatctttc ctgatatagt cattagtctt
     2821 tctgttagtt tttttcgagt taattgaggt aaactatgat tcacgacgac tagaacatct
     2881 acatcgctgt taatgcgtaa accaccattt actgctgaac caaatagata tactccaact
     2941 attgaacttc caaataaatc ttttacgatt tttaatgttt gaatcgcttg atttggtatt
     3001 tttccgttaa tcaaattgct catgatttca cctcgttgat tatgttcata taaagtttat
     3061 attgatactc aatttactta ccctagattg gacatatact taaattactg ttcaataaag
     3121 ctgaccgtta gcgtttaagt acatcctttc acaatttgtc tacagattaa taattattct
     3181 ttattataca gatctccata taatttttga atttggttct gtaatttttt attttctttt
     3241 tctaattcca ttactcttct ttttaaggtt ttaataagga tttcctccga acgagaactt
     3301 ttcttgggtt ttgagactac atttgctgtt atttgacgct cacgaaggga ttcgattctt
     3361 tgcctaatat cgtgttcctt ataaagccat gatttagaaa cattagcttc ctttgctatt
     3421 gaattaaaat taataacttt accttcaatc gaaaatttag aaatcgcttt gtctactttt
     3481 tcccttgtct tttttgattt ctgcttcgcc aaacgtacaa tttctgttgt atttctaact
     3541 tgtttatcca ttgataatta ccccgtcaaa cttccaatga tttgttctaa acgctcttta
     3601 acacggctat tagtctctac ttgtctttgc cattgtttat ccttagctat ggctaataac
     3661 tcttctgtac gctctaactg ttcttcgtgc tgtggtaaga attgcttact ggtacagaag
     3721 tgagtgcaat ctaagcatgc attcgcatgt ggacaaccac ctgctactac tggcaatcta
     3781 caataaccat ttggaagcac ttgtgcattt atatttttct tgaaccattg aagctctaca
     3841 tcatcgactt cattatcttc atctagatca agcacatctc cattattggt aaccagtttt
     3901 tcctgaaatt tagtaaattc attttttaga gtttcatcaa agatatgagc gtatctgctt
     3961 gtcatttctg ggctttcatg ccccaaaaat ttctgcacaa tatgctgggg catcccgttg
     4021 ttaatcattc ttgttcctac tgtatggcga aaggcatggg catggaatct ataaatctca
     4081 cctgatttat ccactatatt ttgctcataa gctaatttat ttaactcacc tctaaatgtt
     4141 tcttgtttta atggcgatcc atcttttctt ggaaagaggt attcactatc tggaaattcc
     4201 tctgaaactt tatcttcccg aactttaata agtaaagcta cctctttaga tattggaact
     4261 atatgctcct ttttcatttt ccattgataa tactttaaaa agaaatctcc atctttgtcc
     4321 tctaatagac agcctttttt caaggtgcac aattcactta tcctcattcc acattcttga
     4381 acaatcatag tcatcgtagc tatatattcg ggtaatttat caagatgact gttcaattgc
     4441 tctaggacga attcatctat aaagcgtggt tttgctcttg gtattttcgg atagtcctca
     4501 gaataaatta atattttgga aggaacatca tcccattcta gcctaagaag ggtactaaat
     4561 agtccttcca atatagagat cctcccagtt attgtactag gttttattcc catcatgttt
     4621 agttcactta aatatgcttc aatttccact ctcgttaatt ggtgtactct ctgaacttgt
     4681 ttaaatttca tgtccagaaa attaaagaac tctttaagtc tttgggcaat atcacttaca
     4741 taggaaaagc tatccacgtt caatctcaac ttacaatatc tttttacaag ttgtttaaaa
     4801 tatgtattcc gaaacccttt aaagttaatt gtatattcat attgtgttgg gttaacctta
     4861 tcatctggca aaggtaagtt acgtctatcc caaacgtctt tatcccactc ctctccatca
     4921 aaataaaagt tctcataaaa ctccataaat tgttttagat tagtaacata gtaggaatta
     4981 gcttttacag gtgttttttc ttgattagca gtaatcttat aattagtagt ggtaattcta
     5041 acaccccgtt ttgtcaaata agttctatac tccgtcattg ctttttcaat aggaacttca
     5101 gtaattgaag taatgctagg atactttaaa tctaagaaat ctaacatttt attaattact
     5161 gttccttttc taatccagac agtttttgca ttccatattc cattgtttaa atggtaaaaa
     5221 taaaaatatt tcaattctgt tcttaaccac agatttttaa cacgttcaaa acgaacccaa
     5281 cgattcctta aagcaggatt cttacttaat tctatggcag aaggatgtgg acattttctt
     5341 atatcccaac tattattagc ccaaaacccc tgcatttctt cattcattac agctattttt
     5401 ttgctaatct cactctgact aataattttc cttttactag aagcattcat ttcttatgct
     5461 cctttctctc gaggtattta ttaaactcat ttttcatatc ctgatctgaa agatgaacat
     5521 aggtatttaa cgttgtctga acatgtgcgt gacctaatct cttttgaacg aacgcaacat
     5581 cccatccttc cctaattagc tgcgttgcgt gagtgtggcg aagcatatgt gatgtaaatt
     5641 ctattccagt ccttttaact attcttctaa ctagatcaag aacactttgg tactttagtg
     5701 gtttcccaaa atagccttct tttaaggaaa taaaaacata atcatgctcc aattcctcac
     5761 tatactcata tatcaagtaa tctgtataaa gtgacataag ttctttactc acatgtattg
     5821 ttctttcctt ccttaattta atataagctt cattaacatt aacatctcta ggtgttaaat
     5881 ggatttgatt gtcccaagtg acaatatctt caagcctaag cgataacact tcaccgattc
     5941 ttaaaccacc ctcatacata agcattaaaa ttaatttatc tcttttcgta tgacaagcat
     6001 caataatttg cttaacttcc tttgatctca atgttcttat ctgtttcttt ttaaccctta
     6061 actttaagac attcttttgg tatctaccct tattaacatg atgtaaaaat cctttgaaat
     6121 ttcttccctt tgcttgttta aatacatcaa ttgatttaaa ttctcctaat ctacttaaat
     6181 aatcaagaaa actcataact acatttaaaa ttgtattcac tgtcgtttct tctcttatgg
     6241 ctttttttga ctgaagatca attacatttg atgctgaagg atatctcaac caacctacga
     6301 agtctgctaa caactcaaag ttaatatcat taagaataac acctctctgt tccatgaact
     6361 cgtacagcaa ctttaaatga tagcagtatg ccttaatggt attaggagac ttaccagtat
     6421 tatctaagta tttaataaat ttcattactg gttctattag ctggtattct ttatctagta
     6481 ataaatacaa tggatacggc ttattctcca cttctatcct ttgaaccttc acatgttccc
     6541 acctctttaa atactttaac tactacaaat tataaacctt gttaattttt atttaaagta
     6601 aaatatccct ttaatatctc ctcttaaact actttaacta ctattattta ttatactatg
     6661 gttaataaaa tcaatgtttg gtggttatga tgaattgatg gaacctgtgt gtaacacatt
     6721 tactgctaag gaaccattta atcaacttgg tggttatcca tattttgacc aaatagatcc
     6781 aagaacgaac gatcaagaac tgaaaatgta tgatagagtc ttactgcaaa ttgattctac
     6841 aagagatggt aattcttcga ttatatgggg tgatttaggt attgccaata tcttagtaaa
     6901 atctactgac cttgaggcta tgaagtttga tgattacatg tattcatggg attgcagcta
     6961 agagaatcat aaaaatatat tactttaata agtgtagtat ctatagatca atctagggag
     7021 tgggacagaa atgatatttt cgcaaaattt atttcgtcgt cccaccccaa cttgcattgt
     7081 ctgtagaaat tgagaatcca atttctcttt gttggggccc atccccaact tgcattgcct
     7141 gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg actagaattg aaaaaagctt
     7201 gttacaagcg cattttcgtt cagtcaacta ctgccaatat aacttcgtag agcatagaac
     7261 attgatttat gtcccagcct gttttcataa gccacagcta atgtgtattt aaaaatagga
     7321 atacatgagt aaaactcatg cataagaaat actaatttct aaagaaaaag tatttcttta
     7381 tgttggggcc ccccaacttg cacattattg taagctgact ttccgccagc ttctgtgttg
     7441 gggccccgct ataattgaaa agtttgttat aggtgtattt tcttttggtt aactattgtt
     7501 aatataacat tgtagatttt aggatgttga tttttgactt agcctttgtc tgcttatgtt
     7561 aaatgttcca gctagtcaaa cattgagttt gtaattatta tattaagcta gtttaaattt
     7621 tttgtgattt atgttttatt cataccaatt ttgtgaaatg atacagtgac agatgtttat
     7681 tgattttaaa gggtgatgat gatggctatt gtaaataaag tgataattgt tgaaggaaaa
     7741 tctgataaaa aaagggtgca acaggttatt gcagaaccag tcaatattat ttgtactcat
     7801 ggaacaatga gtatagataa gcttgatgat atgatagaat cactgtatga taaacaagtt
     7861 tttgtattag ccgattctga tgacgaagga gatcgaatta gaaattggtt taaacgttat
     7921 ttgagtgaaa gtgaacatat atttattgat aaaacttact gtcaagttgc gaattgcccc
     7981 aaacaatatt tggcgcatgt actttcaaaa catggcttta cttgtaagaa agaaacacct
     8041 cttttaccga atataaataa tgaaaggtta gttttagtaa atgaataatt cattagacat
     8101 caaagatgta actacatttt atgaggaaga caaacattta atctttggtt atacaccaac
     8161 gtgtggtact tgtaaggttt cagaaagaat gttagacatt gctaatgaaa tattgcagtt
     8221 accattattg aaaatagatt taaactttta tcctcagttt tgtaaagata tgtaaatcat
     8281 gtctacgccg attttattgt tgatgaataa agataaagaa gtaaaacgaa tttatgcatt
     8341 taaatcggtg actgatttgt tagaaaattt aaaatagtta ttgacgaaat tttactaagg
     8401 tgttaggatt aatacaaatt aaatgatgcg taaactctta tcgagagtgg tggagggatg
     8461 tgccctacga agcccggcaa ccgtcttata tagaaatggt gccaattcac ataaagtttt
     8521 aacttttgaa gatgagagaa acaatactac tattgctttc tcaatttttc tatcgatatt
     8581 gagaaagcat tttttatttt attaagcaac acagggagga atcaacgtga ttgaattaaa
     8641 agaagttgtt aaagaatatc ggactaaaaa taaagaagtc cttgctgtag atcacgttaa
     8701 tttatcgatt cgagcaggat cgatttatgg cgtcattggt ttttctggag caggaaaaag
     8761 tactttgatt cgaatgttta atcatttaga agcgcctaca tcaggtgaag ttattataga
     8821 tggagaccat ataggtcaat tgtccaaaaa tggattaaga gcaaaaagac aaaaagtaag
     8881 tatgatcttc caacatttta atttgttatg gtcaaggact gtgttaaaaa atattatgtt
     8941 tccgcttgaa attgcaggtg tccctagaag gagagctaag caaaaagcat tagaacttgt
     9001 cgaactcgtc ggtttaaaag gtagagaaaa ggcttatcca tcagagttat caggtggaca
     9061 aaagcaacgt gttgggattg cacgagcgtt agctaatgat ccaacggtct tgctttgtga
     9121 tgaggcaaca agtgcacttg atccgcaaac aacagatgaa attttagatc tactactaaa
     9181 aattagagaa caacaaaatt taacaattgt actaattacg catgaaatgc atgtcattcg
     9241 tcgtatttgt gatgaagttg cagttatgga aagtggtaaa gtgatagaac aaggaccggt
     9301 gacacaggtt tttgaaaatc cgcaacacac tgtgacaaaa cgatttgtga aagacgattt
     9361 aaatgatgat ttcgaaacat ctttaacaga attagagcca ttagaaaaag atgcatatat
     9421 cgttagatta gttttcgctg gttcaacaac aaccgagcct attgtatcga gtctatcaac
     9481 tgcctatgat attaaaatta atattttaga agcaaatatt aaaaatacaa aaaatggaac
     9541 agtcggcttt ttagttctgc atattccata tatttcaagt gtagatttcg gaaaattcga
     9601 aaaagagtta attgagcgac aagttaaaat ggaggtgtta agacatgggt aaatcattta
     9661 gtgaaattat aaatgaaatg attacaatgc ctaatattca gtggccagaa gtttggactg
     9721 caatagtcga aacactatac atgacagtcg tctcaactat atttgcattt atacttggtc
     9781 ttattttagg tgtgttatta ttcttgtctg ctaaaggtaa gtctatcggt gcaaggttat
     9841 tttattctat cgtttctttc attgttaact tatttagagc gataccattt attattttaa
     9901 ttttattatt aattccattt acaagtttga tacttggaac gataagtggt ccgacaggtg
     9961 cgttaccagc cttgatcatt ggcgcagcac cgttttatgc aaggctcgta gaaattgctt
    10021 ttaaagaaat tgataaaggt gtcatcgaag cggcttggtc aatgggcgct aatacttgga
    10081 cagtaattcg taaagtcctt ttacctgaag ctatgccagc gctagtgtct ggcattacag
    10141 ttacagcaat cgctttagtt ggttcaacag cagttgcagg tgtaattggt gccggtggtt
    10201 taggaaattt agcatactta acaggtttca ctcgaaatca aaatgatgtc attttagtat
    10261 caacagtttt tattttaatt attgtattta taatccaatt cattggggat tggcttacaa
    10321 ataaacttga taaacgataa attgggggtt tcattttatg aaaaaattat ttggtcttat
    10381 tttagtatta acatttgcag ttgtattagc agcttgcggt aatggaaaca aaagtggcag
    10441 tgacgacaag aaaataacag taggtgcttc accagcacca catgctgaaa ttttagaaaa
    10501 agcaaaacca ttattagaga aaaaaggtta tgaactagat attaaaacaa ttaacgatta
    10561 cactacacct aataaattac tagacaaagg tgaaattgac gcaaactatt tccaacatac
    10621 accatattta aacacagaga aaaaggataa aggttacaaa atcgtaagtg ccggtgatgt
    10681 tcacttagaa cctatggctc tatactctaa aaagtataaa agtttaaaag aattaccaaa
    10741 aggtgcaaca gtctatgtgt ctaataatcc agctgaacaa ggacgtttct taaaattctt
    10801 cgttgatgca ggtttaatta aaatcaaaaa aggcgtaaaa attgaagatg ctaagtttag
    10861 tgatattaca gagaataaaa aagatattaa gtttaataat aaacaatcag cagaattctt
    10921 acctaaaatt tatcaaaatg aagacgctga tgctgttatc attaattcga actttgcaat
    10981 cgaacaaaaa ctaaatccta aaaaagattc tattgctgta gaaagtgcga aagataatcc
    11041 ttatgcaaac ttaattgctg ttaaagaagg acatcaagat gataagaaaa tcaaagcatt
    11101 aattgaagta ttacaatcta aagatattca agacttcatt aatgaaaaat acaatggtgc
    11161 agttattcct gctaaataaa tcatattaat ggctcagtaa cgtgatgttg ctgggctttt
    11221 taatttaaac aggtatttat atgatattta ggaatgagat gctattacgg ataaagtaaa
    11281 atcctatatg catgcgtgct actcatttat tcttattttt aatttcacat ggctagtgac
    11341 taatacatca taaatcatta gtgattcttt attatttcag tcccactccc tcaagttaat
    11401 gatgaatcta tttcgctagt tataaaatga cgtcaaatca atatcaaaaa ttatttaagt
    11461 aaaatgttta gataattttt cagtgggtaa gtattatata taacatttaa ttattccgag
    11521 gaggcattta ttatggcaga cgaaagtaaa tttgaacaag caaaaggtaa tgttaaagaa
    11581 acagtaggta atgttactga taataaaaat ttagaaaacg aaggtaaaga agataaagct
    11641 tctggtaaag cgaaagaatt cgttgaaaat gcaaaagaaa aagcaactga ttttattgat
    11701 aaagtaaaag gtaacaaagg cgagtaattt tactttttat tcctaataca ctgcacatgc
    11761 cacttagtcg gtataaccat cggctaggtg gtttttgtat taaaaaagtg gataccaaaa
    11821 tttattaata attattttaa tgttagaaaa aaactaaata aaaactcgct aatgatatcc
    11881 aataatatgt atacaaaacg agacatatat tgcatatgat taacgagata ctgaaaatat
    11941 tttatcaccc ctaaaatgat tattcatttt cagcggtaat tcgacctaaa gtcaaactta
    12001 caataaaacc gatgataaat actactaatg aaacgaacca catcacgata ttagttggta
    12061 aacctggaaa tactgcaaag agggagccaa caacaaaacc aatgattaat gcaaaagtca
    12121 ttagtttatg atgtgttagg aaatactgga taattttgct tgaaataatg aatccagcaa
    12181 gcacgccaaa tccgactgca agtaatatag gaagacctgc aaagttaagt ttaacaactt
    12241 cagatattgc tagcatgacc gtaccataga cgccaaatac taataacata aatgaccctg
    12301 aaatacctgg gagtaacata gcactagatg cacacatacc tgcaataaaa tatttaataa
    12361 taagactagt tgatagagta agtgtttctc cagcatgttt atcaccatta ttcattaatg
    12421 taataacaat taagatagcg ataccagcta taaccatcat gtaatgttta gttgtaaatg
    12481 acgttttata gttagaaatt ttcaataaat atggaacgat accaatgatt aatccaccaa
    12541 agaaaaacat agttggaata tggtgttggc ttaataaata attaaaaaga ttacttagtg
    12601 atcccattgc cagtaacatt ccaattataa tggggattaa aaatgtaaaa cttggccaaa
    12661 aacgtcgtga gaatatgccg ctaattgaag cgataaattg attgtaaata cctaacaata
    12721 atgcgatagt cccaccgcta acaccaggta ccaagtcact cgttcccata gcaaaacctt
    12781 ttagaatatt aatccattta aattgttgca tgaataactc ctttcaaacg attggaataa
    12841 aatcataaat agcatcatac catattacaa atgtcctagt gaaatgataa catattttaa
    12901 attcataaaa tccattgaga aattatgtgc acttattatc atttatattt ttaaagagag
    12961 cggattagag ttgtacttta gagttattaa aaataataaa aagggtttaa gttg
//
LOCUS       DBNENW010000067        12972 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNENW010000067 DBNENW010000000
VERSION     DBNENW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.067"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            149..440
                     /locus_tag="AC1LW8_001537"
                     /pseudo
     CDS             149..440
                     /locus_tag="AC1LW8_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="AC1LW8_001538"
     CDS             complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="AC1LW8_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HIP4773371.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRMDSKE"
     gene            1624..3225
                     /locus_tag="AC1LW8_001539"
     CDS             1624..3225
                     /locus_tag="AC1LW8_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP4773372.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     regulatory      3835..4010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            4092..5297
                     /locus_tag="AC1LW8_001540"
     CDS             4092..5297
                     /locus_tag="AC1LW8_001540"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIP4773373.1"
                     /translation="MVTRSVLKYGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVAGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            5361..6350
                     /locus_tag="AC1LW8_001541"
     CDS             5361..6350
                     /locus_tag="AC1LW8_001541"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIP4773374.1"
                     /translation="MTKLAVVGSTGLVGTKMLETLNRKNIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            6352..7239
                     /gene="dapA"
                     /locus_tag="AC1LW8_001542"
                     /pseudo
     CDS             6352..7239
                     /gene="dapA"
                     /locus_tag="AC1LW8_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
     gene            7236..7958
                     /gene="dapB"
                     /locus_tag="AC1LW8_001543"
     CDS             7236..7958
                     /gene="dapB"
                     /locus_tag="AC1LW8_001543"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIP4773375.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNIPVFF
                     SANMSYGVHALTKILAAAVPLLDEFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7985..8704
                     /gene="dapD"
                     /locus_tag="AC1LW8_001544"
     CDS             7985..8704
                     /gene="dapD"
                     /locus_tag="AC1LW8_001544"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIP4773376.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8847..9998
                     /locus_tag="AC1LW8_001545"
     CDS             8847..9998
                     /locus_tag="AC1LW8_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIP4773377.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            10003..11088
                     /locus_tag="AC1LW8_001546"
     CDS             10003..11088
                     /locus_tag="AC1LW8_001546"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIP4773378.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLVPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYTDE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            11078..12343
                     /gene="lysA"
                     /locus_tag="AC1LW8_001547"
     CDS             11078..12343
                     /gene="lysA"
                     /locus_tag="AC1LW8_001547"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIP4773379.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGNESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12583..>12972)
                     /gene="msaC"
                     /locus_tag="AC1LW8_001548"
                     /gene_synonym="msa"
     CDS             complement(12583..>12972)
                     /gene="msaC"
                     /locus_tag="AC1LW8_001548"
                     /gene_synonym="msa"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator MsaC"
                     /protein_id="HIP4773380.1"
                     /translation="ILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGILFFK
                     TNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKDFGFD
                     EILFYTLYLLLILIVLYYLKKQVKHKI"
BASE COUNT         4532 a         2038 c         2351 g         4051 t
ORIGIN      
        1 ggtgccgtct cagccacccc aaccggcaca ttgttgtaag ctgactatat gtcagcttct
       61 gtgttggggc ccctgtcttc gactggcact gctccctcag gagtctcgcc attaatacta
      121 cgtattaaca tgtaatttta ctttgaaata cattaaagcc caaattaatc aaaagctttc
      181 tgaaccagaa acgaaaaaaa atctatagtc atagaaaaat ttatgtagag cctgtttttg
      241 gatttatgaa ggttattttg ggtttcactc gaatgtcagt tcgaggaata aataaagtta
      301 aacgagagct aggttttgta ttaatggcac ttaatataag gaaaatagca gctcaacgag
      361 ctgtacatta taaaatacat atcaaaaaag ctaatttcca tcaaataatt aatagaaatc
      421 agctttttta cattgcctaa gaacttaatg tcccagccta ttcgtttgaa ttatttaatt
      481 ttttactttg aaaggaatgt ttaaaaaact acttcatctg tccgatttaa tattgaataa
      541 aaaaacacgc atcctacgac acgtgtaaaa atgattattc ttttgagtcc attcgactcc
      601 aacctttttt agttaaagtg attttacctg tttctatatt aataatcttc tgtttatata
      661 agtgaccgat tgcacgtttg aatgaacctt tactcatatt gaatacttct ttaatcgctt
      721 cagggcttga tttgtcccag aatggtaatt caccatcata ttcaactagt aaatcaaaga
      781 tgacttggcc gtcatcgtct aaacgttcat gtgcaagtgg taaaaatgaa ccatttaact
      841 cacctttatc attatgcccg ataattctaa cttgaacaga ttcacctaat cttggttcag
      901 ctttacgttc tgattcatgt acgaaaattt tgtaacctga ttcgcttaat aaaaagctac
      961 caattcgtaa tacgcggtaa ggtttggctt caatgacttc gttttttaaa ttatcgtcgt
     1021 gtacaggtgt aaacatattt tctacaacag attcactcgc taatcttcca tacatatgat
     1081 tctcacggtc aattcgtaat gtgactagca aatgatcacc aggttgtggc catagtgatt
     1141 tcacttttgg taaatcttcc catggtacta acacttcacg gggtaatcca acatctatac
     1201 gtgccccatc gcgatccgtt ttaagtactt tagcaaagtc atatttatct ttcgtaatat
     1261 caggcatatt ttgagttgca aataattcac ctgaacggtt tggataaatg aagaaactat
     1321 attcttcacc tacttctaat tcatcatcat cgttcatttc tgattggttt aactttacgt
     1381 tttcaccgtt tggtcctttt aaaaggtaag ttgagccttg taaccctact acttcaagga
     1441 attctataga acctactata tctttgtcta atgccatgtt attctccttc gattcttgta
     1501 ttctttatta ttataacatg tgtgagtcat tctatcatta caacttatat gaggtcgaaa
     1561 ctgtgacgat aatatgatat aattcttccg ttagaatatc gaatgaaaaa aaggagaaaa
     1621 tgcatgttac aagtaactga tgtgagttta cgttttggag atcgtaaact atttgaagat
     1681 gtaaatatta aatttacaga aggtaattgt tatggattaa ttggtgcgaa tggtgcaggt
     1741 aaatcaacat tcttaaaaat attatctggt gaattagatt ctcaaacagg acatgtttca
     1801 ttaggtaaaa atgaacgtct agctgtttta aaacaggacc actatgctta tgaagatgaa
     1861 cgcgtgcttg atgttgtaat taaaggtcac gaacgtcttt atgaggttat gaaagaaaaa
     1921 gatgaaatct atatgaagcc agatttcagt gatgaagatg gtatccgtgc tgctgaactt
     1981 gaaggtgaat ttgcagaaat gaatggttgg aatgctgaag ctgatgctgc taacctttta
     2041 tctggtttag gtatcgatcc aactttacac gataaaaaaa tggctgaatt agaaaacaac
     2101 caaaaaatta aagtattatt agcgcaaagt ttattcggtg aaccagacgt actattactg
     2161 gatgagccta ctaacggtct cgatattcca gcaatcagtt ggttagaaga tttcttaatt
     2221 aactttgata atactgttat cgtagtatcg catgaccgtc atttcttaaa taacgtatgt
     2281 acacatatcg ctgatttaga ctttggtaaa attaaagttt atgttggtaa ctatgatttt
     2341 tggtatcaat ctagtcagtt agctcaaaag atggctcaag aacaaaacaa gaaaaaagaa
     2401 gaaaaaatga aagagttaca ggactttatt gctcgtttct cagctaacgc ttctaaatct
     2461 aaacaagcaa caagtcgtaa aaaacaactt gagaaaattg aattagatga tattcaacca
     2521 tcatcaagaa gatatccttt cgttaaattc acgcctgagc gtgagattgg taatgactta
     2581 ttaatcgttc aaaatctttc taaaacgatt gacggtgaaa aagtattaga taacatttca
     2641 ttcacaatga atccaaatga taaagcaatt ttaattggag atagtgaaat cgcgaaaaca
     2701 acattactta aaatattagc tggtgaaatg gaaccagacg aaggttctta taaatggggt
     2761 gtaacaacat cattaagtta cttccctaaa gataactcag aattctttga gggcgttaat
     2821 atgaaccttg tagattggtt aagacaatat gcaccggaag atgaacaaac agaaacattt
     2881 ttacgtggtt tcttaggtcg tatgttattt agtggtgaag aagttaagaa aaaagctagt
     2941 gtgctttcag gtggagaaaa agtacgttgt atgctaagta aaatgatgtt atcaagtgcg
     3001 aatgtacttt tacttgacga acctactaac cacttagact tagaaagtat tactgctgtc
     3061 aatgatggtc ttaaatcatt taaaggttct atcatcttta cttcttatga cttcgaattt
     3121 atcaacacga ttgcaaaccg tgttatcgat ttaaataaac aaggcggcgt ttcaaaagaa
     3181 attccatatg aagaatactt gcaagaaatc ggcgttttaa aataaacata atatgtagaa
     3241 tttcttttcg aaaatctctg tgttgaggcc cgaccaactt gcacattatt gtaagttggc
     3301 ttactgtcac cttctatatt gggacttatt ccgcaacttg tattgtttgt agaaattagg
     3361 aatccaattt ctctatgtgt ggcaccgaca aggattgaaa aaagctagct acaaagtcat
     3421 tttcattcag tcaactactg tcaatataac attgcagggc ctagggcatt gattcatgtc
     3481 cgagactata ttcaatttaa tagacattaa ctgggcgtct cgatgctaat atttatagca
     3541 tcaaggtgtc ctttttattt tattttttag cacatcagca cagtgaacac cccttctcta
     3601 aattacaaat acagttatca ccgcattaaa agagtacatt tatttaatgt gcatattgag
     3661 tatggagtat gctataagta ttttgaattt tatttttcaa aaagtctcat tatcaccacc
     3721 ttttaaaata taagcaacgc tataacttga attttcaggt tattccaaaa aatctaaatt
     3781 atcggtttac attcatattt gtcatatgta taatgaacac ataccaattt aataatattt
     3841 tgatgaggcg catcaatcat gagtaaagtt tagattactg tctgctaaca gctaaatttg
     3901 aaagggtgcg atgccgaagc gattataata gcagttataa tttgttggac tttttggtta
     3961 agagctgaga gtttgtcatt atttaaaaat aatggagtgc atcacttgta tatagattaa
     4021 gagcaagttc gcattccgaa cttgttcttt ttttattatt gtgtgccctt cctaacaatt
     4081 aggaggatta tatggtaaca agaagtgtgt tgaaatatgg cggatcatcc gtcagtgatt
     4141 ttacaaaaat aaaaaggatc gctgaaatgt taaaggagcg ggtcaatcaa gatgaacaat
     4201 taattgtcgt tgtaagtgct atgggtaaca caacagatca attaatgacg aatgtatcga
     4261 ccttgactaa agcaccaaaa caacaagaac tggcattatt attgacaacc ggagagcaac
     4321 aaactgtatc ttatttatca atggtattaa atgatatcgg tgtgaatgcc aaagcaatga
     4381 ctggctatca agcgggtatt aaaaccattg gccatcattt aaaaagtaaa attgctcaaa
     4441 ttaatcctca aacatttgaa caagcctttc aagaaaacga tattttagta gttgctggat
     4501 ttcaaggcat caatgaacat caggaattaa caactttagg cagaggcggt tctgatacga
     4561 ccgctgtggc acttgctgtt agtaatcaaa taccttgtga aatttatacc gacgttgatg
     4621 gtgtgtatgc cactgaccca agacttttac caaaagctaa acgactagac atcgtctcat
     4681 atgaagaaat gatggaaatg agcgctttag gtgctggtgt acttgaaaca agaagtgttg
     4741 aattagctaa aaactataat atccctttat atttaggaaa aactttatcg aacgtgaaag
     4801 gaacatggat tatgtcaaat gaagaaatat tagagaaaaa agcagttgct ggtgtggctt
     4861 tggataaaca tatgatgcat gtaacaatta gttatcccct acctgacaat cagctactta
     4921 cccaactatt tacggaactt gaagaaggtg ctgtaaatgt tgatatgatt tcacaaatcg
     4981 tcaacttgga tggactacaa ctatccttca cgattaaaga tagtgatttt catcaaattt
     5041 ctatgattct tgaaacatta aagaatcaat atgaagcatt agcttataaa atcaatgagc
     5101 attatgtcaa aatttcatta attggctcag gcatgcgtga tatgtcaggt gtggcatcaa
     5161 aagcattttt gacattaatt gaaaataata tacctttcta ccaaacaaca acttctgaaa
     5221 taagtatttc atacgtcatt gatgatttta atgggcaaca agcggtggaa aaactatatg
     5281 acgcatttaa catttaatgg taaaatgatt gttaaaatat tctaaaaatt ggaaattatt
     5341 ataaaatgga gtgacaagtt atgacaaaat tagcagttgt gggttcaaca ggattagtag
     5401 gtacaaaaat gttggagaca ttaaatcgta aaaatattcc tttcgatgaa ttagtattat
     5461 tttcatcagc acgttctgca gggaaagaag ttgaatttca aggaaaaaca tatacagttc
     5521 aagaattaac tgatgctcgt gcaagtgaac atttcgatta tgtattaatg agtgctggtg
     5581 gcggtacaag cgaacacttt gccccactct ttgaaaaagc tggtgcaatc gttatagaca
     5641 attcaagtca atggcgtatg gcagaagata ttgatttaat cgttccggaa gtcaatgaac
     5701 ctacttttac aagaggtatc attgccaatc caaactgctc tacgattcag tctgttgtgc
     5761 cattaaaagt attgcaagat gcttatggtt taaaacgagt ggcatataca acatatcaag
     5821 ctgtatcagg ttcagggatg aaaggtaaga aagatttagc tgaaggtgta aatggtaaag
     5881 caccagaagc atatccacat ccaatttata ataatgtgtt accgcatatt gatgtgtttt
     5941 tagaaaacgg atatacaaaa gaagaacaaa aaatgattga tgagacgaga aaaattttaa
     6001 atgcgccaga cttaaaagta acagcaacat gtgcacgtgt gcctgttcaa gatagtcata
     6061 gtgttgaaat tgatgtaacg cttgaaaaag aagcgactgc agaagatatt aaagcattat
     6121 ttgatcaaga tgaccgcgtt gttttagtag acaatccaga gaacaatgaa tatccaatgg
     6181 caatcaattc tactaataaa gatgaagtgt ttgttggccg tatacgtaga gatgattcat
     6241 tagaaaatac tttccatgta tggtgtacat cagacaattt attaaaaggt gctgcattaa
     6301 atgctgtaca agtattggaa caagttatgc gtttaaaagg agcgaattaa aatgacacat
     6361 ttattttagg gtgttggcgt tgcacttaca acccctttta caaataacaa aattaatatt
     6421 gaagctttga aaacacacgt taatttttta ctagaaaata atgcccaagc aatcatcgtt
     6481 aatggaacta ctgctgagag ccctacttta acaacagatg aaaaagaacg cattctaaaa
     6541 acagttattg atcttgtaga taaacgtgtt cctgtcatag caggaactgg cactaatgat
     6601 actgaaaagt caatccaagc ttcaatccaa gctaaagcct taggggctga tgcaattatg
     6661 ttaattacgc cctactacaa caaaacgaat caacgtggtt tagtcaaaca ctttgaagcg
     6721 attgcagatg ctgtgaaatt accagtcgtg ctgtacaatg ttccttcaag aacgaacatg
     6781 acaattgaac cagaaactgt agaaatatta agtcaacatc cttatatagt tgctttaaaa
     6841 gatgctacga atgattttga gtatttagaa gaagtgaaaa agcgaattga tacaaattca
     6901 tttgcattat atagtggcaa tgatgacaac gtcgttgaat actatcaacg tggcggtcaa
     6961 ggggttatct ctgttattgc caatgtcatt cctaaagaat ttcaagcgtt atacgatgct
     7021 caacaaagtg gattagatat tcaagatcaa tttaaaccaa tcggcacact gttatcagcc
     7081 ctatcagttg atattaaccc aattcctatt aaagctttaa caagttattt aggatttgga
     7141 aattatgaat tacgtttacc attggttagc ctagaagata cagatactaa agtgcttcgt
     7201 gaagcatatg acacatttaa agcgggtgaa aatgagtgaa aatattacta attggctatg
     7261 gtgcaatgaa tcaacgcgtt gctagattag cagaagaaaa aggacatgaa atcgttgggg
     7321 tcattgaaaa tacaccgaaa gcaacaacgc catatcaaca atatcaacat attgcagatg
     7381 ttaaagatgc cgatgttgca atagattttt caaatccaaa tctgcttttc cctttattag
     7441 atgaagagtt tcatttgcca ttagttgtgg caacaactgg cgagaaagaa aaactactta
     7501 ataagttaga tgaattgagt caaaatatac ctgtattttt cagcgcgaac atgagttatg
     7561 gcgttcatgc actgactaaa attttagcag ctgctgttcc cctacttgat gaattcgaca
     7621 tcgaattaac tgaggcacat cataataaaa aagtagatgc accaagtggt acgttagaaa
     7681 aattgtatga tgtgatcgta tctttgaaag aaaatgtaac acctgtgtat gatagacatg
     7741 aattaaatga aaaacgccag ccacaagata ttggtataca ttctattcgt ggaggtacga
     7801 ttgtcggtga acatgaagtt ctatttgctg gcactgatga aacgattcaa atcacgcatc
     7861 gtgcacaatc aaaagatatt tttgcgaatg gtgcaataca agcagcagaa cgcttagtta
     7921 ataaaccaaa cggcttttat acgtttgaca acttataaac atattaaagg agattgatta
     7981 ttttatggta caacatttaa cagctgaaga aattattcaa tatataagtg atgctaaaaa
     8041 gtctacacca ataaaagtat atttaaatgg taattttgaa ggcatcacat atccagaaag
     8101 ttttaaagta tttggttcag aacaatctaa agtaatcttt tgtgaagcgg atgattggaa
     8161 acctttttac gaagcatatg gtagtcaatt cgaagatata gaaattgaaa tggatcgtcg
     8221 caattctgcc attccattaa aagacttaac aaatacgaat gcacgaattg aaccaggtgc
     8281 gtttattaga gaacaagcca ttattgaaga tggtgctgtc gttatgatgg gcgcaacaat
     8341 taatattggc gcagtcgttg gcgaaggtac aatgattgat atgaatgcta ctctcggtgg
     8401 tcgtgctaca actggtaaaa atgtacatgt aggggctggc gcagtattag caggtgtgat
     8461 tgaaccccct agtgcatcac cggttataat cgaggatgat gtattaatcg gtgcaaatgc
     8521 agttatttta gaaggtgtac gtgttggtaa aggtgctatt gttgcagctg gcgcgattgt
     8581 gacacaagat gtaccagctg gtgcagttgt tgctggtaca cctgcaaaag tgattaagca
     8641 agcttctgaa gtacaagata ctaaaaaaga gattgtagca gcattaagaa aactgaatga
     8701 ctagtgacgt caacgtataa taatttcaag gttgagatac gattatgtct caaccttatt
     8761 gtttatcatt gaataaactt atgatcacaa atttcgaaat acgttacact atttatatag
     8821 taatttaaca caaaggattg ataattatga atgaattaga atttgttacg aaacatcgcc
     8881 gtcatttaca tcaacatcct gaattaagct tacatgaatt tgaaacaact gcttatatta
     8941 aagcgttttt agatagttta aatattaaat acgattgccc attggaaact ggcgtcattg
     9001 catacttaga aggtaatggc tcacatacga tagcgtatag agctgatatt gatgccttac
     9061 ctattttaga ggaaaatgat gtgccttatc gcagtcaatc tgatcatgtg atgcatgctt
     9121 gtggacatga tggtcataca actgcattaa tgctttttgt acaacgttgc aaagacatgc
     9181 aagacgcagg tcaattaccg caaaatgtcg ttttcatttt ccaacctgca gaagaaactg
     9241 gtggcggtgc aaatcgatta ataaaagccg gtgcctttga taagtatcca attgaagcgg
     9301 tatttggtat tcatgttaac ccatttgctg atgaaggcat tgcagtgata agagatgaag
     9361 aaattacggc cagcgcaaca gagtatcgct ttttcttaac aggcctgtca agtcatgttg
     9421 ctgataaaga acaaggtcat tcttgtggtg aagcattaca acatgtatta actcaaatat
     9481 cacaaattca acaatttcac cttaacggtt tgaaacgaaa tattgttcat attggtcatt
     9541 ttaaagctgg tgaagcgatt aacactgtac caagtaatgg ttatttagaa ggtacaattc
     9601 gtacatatga tattgatgat ttaacaatcg ttaaaaatca aatgcacaag atagcagaaa
     9661 gtgtcaagct tctgtttaat gtagattgtg aagttaaatt tgcagaaggt tatcccccta
     9721 caatcaatag tccgaaatta cgtactcaaa tagaggacgc cttaataaaa gctgatttaa
     9781 atgtctatga caaaccaacg ccattcttat ttggggaaga ttttagtttt tatggtcaac
     9841 aactagctcc agcttacttt gtttttatag gaacacgaaa tgaagataaa ggttttgtaa
     9901 ctggtttgca cacatcacat ttaaattttg atgaaaaagt gttaataaac gtggttaatt
     9961 tttacgaaaa tttattaaat aattacaaag aggtgtaata cattgacagc aacatggtct
    10021 gtaaataaga aaatattttt acaaaatgca atcacagtca aaaacaatca gccattaatg
    10081 gcagttgtta aaaataatgc atatcactat gacctagaat ttgctgtaac tcagtttatc
    10141 catgcaggta tagatacatt tagcacaaca tcactacgag aagcaattca aattagacaa
    10201 cttgttccag atgcaacaat ctttttaatg aatgcagttt acgagtttga tttagtccgt
    10261 gaacatcaaa tacacatgac tttgccgtcg ttgacatatt actataacca taaaaatgat
    10321 ttagcaggta ttcatgttca cttagaattt gaaaatttat tacatcggtc tggatttaaa
    10381 gatttaaacg aaattaaaga agtattgaaa gatcaccatc ataatcaaaa tgcaaaaatg
    10441 attattagtg gtttatggac ccattttgga tatactgatg aattcgatgt gtcagattat
    10501 aatgttgaac gttcacaatg gatggaaatt gttgaagcac ttttatctga aggttatcag
    10561 ttcgacctaa tccatgctca aaatagtgcg agtttttatc gggaaggaca aatattacta
    10621 ccccaccata cacatgcacg tgtaggtatt gcgttatacg gttcaagacc atatagttca
    10681 ctgaatcaac atgatatagt tcagtcatta actgtaaaag cacatgttat tcaagtgcgc
    10741 gaagtacaag ctggtgatta ttgcggttat agctttgcct ttgaagtgac taaaaacaat
    10801 acaaaattag ctgtagttga tatcggttat ggcgatggaa ttttaagaac acgtgctaaa
    10861 catgaagcac ttatcaatgg taaacgctac ccgatacgtg cattaatgat gagccatatg
    10921 tttgttgaag tagatggcaa tgtacatgca caagatgaag ttattcttta taataatgat
    10981 atccgcatcg atgaatatac ctttaaaggt gttggtgcaa attctgaaca attaagtgct
    11041 atgaatcatg attctttaaa aaaggagtac atttcaaatg actgttaaat ataatcaaaa
    11101 tggcgaatta acaatggatg gtattagttt aaaaacgatt gcgcaaagct ttggtacacc
    11161 taccattgtt tatgatgaac tacaaattag agaacagatg cgccgttacc atcgcgcatt
    11221 taaagatagt ggattaaaat acaatatttc atacgcctca aaggcattta cttgcattca
    11281 aatggtcaaa cttgtagctg aggaagattt acagttagat gttgtttctg aaggtgaatt
    11341 atatacagct ttagaagcag gttttgaacc gagtcgcatc catttccatg gtaacaataa
    11401 aacgaaacat gaaattaggt atgctttaga aaataatatc ggttattttg ttatagattc
    11461 attagaagaa attgaattaa tagaccgcta tgctaatgat acggtgcaag ttgtattacg
    11521 agttaatcca ggtgttgaag cacatacaca cgaatttatt caaactgggc aagaagatag
    11581 taagtttgga ttatcaattc aatatggctt agctaaaaaa gcaattgaca aagtccaaca
    11641 atctaaacac ttaaaattaa aaggtgtaca ttgtcatatt ggttcacaga ttgaaggtac
    11701 agaagcattt attgaaactg ctaaaattgt tttacgttgg cttaaagagc aaggcattca
    11761 agttgaatta ttaaaccttg gtggtggctt tggtattaaa tatgttgaag gtaacgaaag
    11821 tttccctatc gaaagtggta ttaaagatat tacagacgca ataaaatccg aaattaaagt
    11881 tctaggtata gatgcaccag aaataggtat tgaaccggga cgatcaattg taggtgaagc
    11941 tggcgttact ttatatgaag ttggaaccat taaagaaatt ccagagatta ataaatatgt
    12001 ttcaatcgat ggcggtatga gtgatcatat cagaactgca ctttatgacg caaagtatca
    12061 agcattgctt gttaatagaa atgaagaagc agatgacagt gtaactatag ctggaaaatt
    12121 atgtgagtct ggtgatatca ttattaaaga cgctaaatta ccttcatcag tcaaacgtgg
    12181 agactatctt gctatattat caactggtgc atatcattac tctatggcat ccaattacaa
    12241 tcaaatgcaa aagccttctg tgtttttctt aaaagatggc aaagcacgtg aagttataaa
    12301 gcgacaatcg ttaagacaac tcattattaa tgatacaaaa taaaaataaa caaagtaatc
    12361 cccgaagcac agaaaattat actgtgactc ggggaatttt tatgcattat agatgactgt
    12421 agaaaacatg cttggacata tactctaaaa aaggaagaac agcacatttt tagttagaaa
    12481 gtatttattt ctttttgtca ttaatccttt atatttagtt agccctattc aactaattta
    12541 aagcatcata tacataccac actttaaaca agcatcatga aattagattt tatgctttac
    12601 ttgctttttc aaataatata aaacgataag aattaacaac aaatataatg tataaaacag
    12661 aatttcatca aatccaaaat ctttacttgt aattaataca tcaatatctt ctaattccat
    12721 catctctgct ttaatataat tattaaactt tggcaaagtc attaaatgta cattgtaaat
    12781 atagtaataa aagtcgataa atatgatgtt aaaaaatatg tatgttttat caatattagt
    12841 tttgaaaaat aatatcccag aaatcattat cggaatcact aatacaatca tcattgctgc
    12901 taaaatatta atatttaatc caattgcaga taatacaccg aaaactaata aattagcaac
    12961 taaactaagg at
//
LOCUS       DBNENW010000068        12740 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNENW010000068 DBNENW010000000
VERSION     DBNENW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.068"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(192..1058)
                     /locus_tag="AC1LW8_001549"
     CDS             complement(192..1058)
                     /locus_tag="AC1LW8_001549"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIP4773381.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDVYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMNKILQDTTS"
     gene            1276..2340
                     /locus_tag="AC1LW8_001550"
     CDS             1276..2340
                     /locus_tag="AC1LW8_001550"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIP4773382.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGTLELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2495..2770
                     /locus_tag="AC1LW8_001551"
     CDS             2495..2770
                     /locus_tag="AC1LW8_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773383.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2956..3678)
                     /locus_tag="AC1LW8_001552"
     CDS             complement(2956..3678)
                     /locus_tag="AC1LW8_001552"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIP4773384.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4032..4481
                     /locus_tag="AC1LW8_001553"
     CDS             4032..4481
                     /locus_tag="AC1LW8_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4773385.1"
                     /translation="MTALFPYIAFENSKEAIAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4562..5149)
                     /locus_tag="AC1LW8_001554"
     CDS             complement(4562..5149)
                     /locus_tag="AC1LW8_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP4773386.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(5127..5235)
                     /locus_tag="AC1LW8_001555"
                     /pseudo
     CDS             complement(5127..5235)
                     /locus_tag="AC1LW8_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5292..6974
                     /locus_tag="AC1LW8_001556"
     CDS             5292..6974
                     /locus_tag="AC1LW8_001556"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIP4773387.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQHQVQLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAVSEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7072..7554)
                     /locus_tag="AC1LW8_001557"
     CDS             complement(7072..7554)
                     /locus_tag="AC1LW8_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP4773388.1"
                     /translation="MIVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPDANYPGP
                     VSDIARVKKLDNLIDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQ
                     QSIHLDSKSKSVHKQQLKELPKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ
                     "
     gene            complement(7654..8037)
                     /gene="panD"
                     /locus_tag="AC1LW8_001558"
     CDS             complement(7654..8037)
                     /gene="panD"
                     /locus_tag="AC1LW8_001558"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIP4773389.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIQNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(8039..8890)
                     /gene="panC"
                     /locus_tag="AC1LW8_001559"
     CDS             complement(8039..8890)
                     /gene="panC"
                     /locus_tag="AC1LW8_001559"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIP4773390.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(8883..9701)
                     /gene="panB"
                     /locus_tag="AC1LW8_001560"
     CDS             complement(8883..9701)
                     /gene="panB"
                     /locus_tag="AC1LW8_001560"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIP4773391.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            9774..10634
                     /locus_tag="AC1LW8_001561"
     CDS             9774..10634
                     /locus_tag="AC1LW8_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIP4773392.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(10720..11424)
                     /gene="budA"
                     /locus_tag="AC1LW8_001562"
     CDS             complement(10720..11424)
                     /gene="budA"
                     /locus_tag="AC1LW8_001562"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIP4773393.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFSLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQNIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(11702..12661)
                     /locus_tag="AC1LW8_001563"
     CDS             complement(11702..12661)
                     /locus_tag="AC1LW8_001563"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIP4773394.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
BASE COUNT         4107 a         2421 c         1940 g         4272 t
ORIGIN      
        1 ttattgaaca aaaagttata aaataacttt tattgcaact caaccttgtt ttacattaca
       61 gaaattaaca atagcgatta aaattttatt ttccaacaat aaaaaagcac aaatattttt
      121 attgaggcag ctgtatcgct tctcattgta aaaatatctg tgcatattat tatctcaatc
      181 taacgtaata actaacttgt tgtatcttgt aaaatcttat tcatagaatg cgcaacacta
      241 tcacggtaca tttcaccaaa tttcaataaa tggaccatca ataaatataa acgataaaat
      301 tcaagtctat aggatgcacc ttttgcgagt ggataatgtt tattatacgc atcataaaat
      361 tcgctcgtaa aaccaccaaa taccgttgta ataccgatat cgaattctct gtcaccatat
      421 aatggtgctg gatcaaataa tgcaggacgt ccatctttta aaaacatata attaccaccc
      481 cataggtcac catgtaataa agacggttta ctttgatgct tttctaattc cgccacaatt
      541 tgacgtcgca ctttgtcata tactttgata tcgttggcat cccatagccc tctatttagc
      601 aactcatctt tcaaatggtc caagcgcttg tcgacaaaaa tggtacacca atcgtcttgc
      661 caatgattat caaaagaaat atcgccacct tcataaggta atgagaagcc aaatttgcct
      721 tcttcttgtt gctgactgtg taattgagct acgagttgcc ctaattggcg ttgactccct
      781 gaagcccctt cttctaaata cgtcatcact aaatacacat caccgttaac ctcgccactt
      841 gcaattactc taggtgccgt gatacctgca cgttcaaatt catttaaacc tgcaatttct
      901 gcagcataaa atgattcttt acgtccacgt tggacaagta agaaaaatgt atccgtatct
      961 gtttcgactc gatatgcttc gtttacatca ccaccactca ctggtgaaat ctctttaata
     1021 tcttttaaag gtaaatgctc taaccattgt tcattcatac aaacaccttc caatctactt
     1081 actttatact ataatattgc ctaattagga tgatttaatc ttaaaaacgt tattcaaact
     1141 tgcgtcaatt ttcaaaactt tttagtttta actaggaaat tatgtaggga aatgctagtc
     1201 tattaaaaga atatatttca catatattaa gttgaggata gtaagcctca aatataatga
     1261 aataggagca tactcatgta caaattaatt aaacctttct tattcaaaat cgaacccgaa
     1321 aaagcacacg gactaactat cgatgcatta aaaacgttac aaaagtttcc ggttttattc
     1381 ccagtcgtcg ataaactatt tacttataag aatccaacgt tatcacaaac gatacaaggt
     1441 aatacgtacg acaatccaat tggcttagca gctggtttcg acaaatcttg cgaagtacca
     1501 aaagcattgg aacaccttgg attcggtact ttagaattag gtggtatcac acctaaacct
     1561 caaccgggta accctcaacc acgcatgttt agattattag aagatgatgc cttgataaat
     1621 cgaatgggct tcaataatat tggtatgaac aaagcactca gtcatttgcg taaaaatgct
     1681 tatcaagtac ctgttggtat caatgttggt gtgaataaaa tgacacctta tgaagcgcgt
     1741 tatcaagatt atataaaagt cattgatacg tttaaacacg acgtttcatt tttcacagtc
     1801 aatatcagtt ctccaaatac tgaaaacctt caaaatttcc atgataaaga tgaattttca
     1861 atgttatgtc aagctttgac aacatttaaa aaacaacatg atgtaacagt gccaatttat
     1921 ttaaaactaa cgtctgatat ggatttcgat ggcttaaaag cactattacc agcgattact
     1981 gagacatttg acggtatcat cttagcaaac acaacgcgac aacgagatgg tttaacttct
     2041 gctaataaag tcgaagaagg cggtttgagt ggtcgtccat tatttgaacg taatttaaaa
     2101 ttgattaagt atgcttatca gcaaacaaat ggtgaatttt taattatagg tacaggcggc
     2161 gtattcagta ctgaagatgc aatcaaaatg atgcgtcacg gtgcgtcact tattcaaatt
     2221 tattcatcac ttgttattga aggcccaggt ttaactaaga aaatgaacaa aggcatcgca
     2281 cgttacttaa aagatcatca ttttgacaat gtcagtgata ttataggact agatgcctaa
     2341 tccaacatag tcaaataata ctttaaaact tgctgaatcg tcttattaaa attatgatat
     2401 aactcaagaa gatatattta tcaccatttt tcaaaataat ggcttaaata tcacatatca
     2461 ttaaatatag cgttttataa cggaggcgtt tgttatgact aaagaaacag ataataaaaa
     2521 aacagtagcg acttctgaca acaaagacac acagcaacaa aatcaacctg aagaagatga
     2581 acgtcaaggt ttgaatggtt atcgtaaaac agatcttgat ttagaaatcg agcaagagct
     2641 acgtgaaatg atgaaaacag gagaaaatga aacgaaaaat gactataaaa agtttaaagt
     2701 gttttcactt atttcaacac ttgtcattgt cattttagca attataagat ttgttcataa
     2761 aatgatgtaa ttagagtgag acattgtttt atgtctcagg atccagttat tcattatatc
     2821 tacaatattt acgattatat aaataacccg agattttagt atgattcatt tcactaaaat
     2881 ctcgggtttc tatttgataa tttttaatgg gatatggcat gtatacgttc ttgcctttta
     2941 tctcatttcc aatgattaat ctggatattg ttctaaaaat gctttcgctt ctttattaac
     3001 tgttttaaaa tcaatacctt gttgcatcgc tgcaaagaca catccacaat aacactgcct
     3061 aaagatatta tagtcattac acatttctat ggaacgctca taacctttac ttttcttaaa
     3121 atcacttggc aaatagttca catcgtatat tttttggaca tccataccaa gttcattgat
     3181 taattgtgcg ttctttttag gtgataatgt gattgcactg ccaaaataat catagccatg
     3241 ttctacagct gctttcgcta caatatccaa acgcatttca aaacaagcgg tacaacgtaa
     3301 gccgccttct ttttcatcag ctaattcttt atccttcacc attttcacaa acttatgcgg
     3361 ttcataaggt gcttcaatat acttcacatt cgcaccagtc ttgcgattaa aatcttccac
     3421 aaattgttct tgcactttag cacgtcgtaa gtactcattt ttcggatgaa tatttgaatt
     3481 cgcgaaataa attgcaatgt ctgcatattg tgttaaaaac tctaatgtat atgtactaca
     3541 aggtgcacaa caactatgca ataagatttt aggtctgatt gcttctcttt cccactgccc
     3601 gattaatttc ttcaacacct tgtcataatt aattttttga tttttcattt tactgataat
     3661 aggttcagca ttaatcatga tttaacctcc cacatttaat cattaacttc tattatatat
     3721 gattcatatt aaatgtcagt caaaaaagtt agaaattcat tttaatgcat ttatatttcg
     3781 aaaatccctt atgtatctaa aagcattttt taaacttgaa ttttaaaaca ctaaacaaca
     3841 catacgtctc tgtgtcattt tcattttttg tatgtcatat atatgtttac ttcatttaaa
     3901 tcaatttcat cttataattt atcgtgtatt ttacaaaaga ttgacttcaa ttcatcgtaa
     3961 aagttatact tttgccattt tttaatgtaa catggtgtta gtaataaaaa taatacattg
     4021 aggtgtttta tatgacagca ttattccctt atatcgcttt tgaaaattca aaagaagcca
     4081 ttgcatatta cgaagaagta tttggtgcaa ctgacgttaa acgtttagaa gttggcgaag
     4141 aacaagcgtc acattttggc atgactaagg aagaagcgca agaagcaact atgcatgctg
     4201 agtttgaagt gcttggcgta aaagtgctat gttctgattc ttttggtcgc gctgacaaaa
     4261 ttaataatgg catatcatta ttaattgatt atgatgttaa caataaggaa gatgctgata
     4321 aagttgaagc attctatgag caaattaaag atcattcttc aattgaaata gaattaccgt
     4381 ttgctgacca attctggggt ggcaaaatgg gcgtctttac cgataaatac ggtgttcgtt
     4441 ggatgttaca tggtcaagac tatactgcaa tccaacaata atgttattga taaagatatc
     4501 atttaataag cttatgttat ttagcaaggc aagccactct cgattcatgg cttgccttat
     4561 tttattgtat atttttaatt atcgttgctg gcccttgatt gacaatcgta ttaaaatgcc
     4621 ttattaagtc aactttgtct atacggtttg gatcttctac ccaatgtctt ataaaagaca
     4681 atcccgcacc tgaaacataa ctcatgaaat aagaaaatgg tataccatta atttgatcat
     4741 tttcattttt ataacgttgt aaatgacaca taattaaatc ataaagtttt tcgtggatat
     4801 ttgaatcttt acccatacga aacatcaatt gataaaatgc gatgtctttt tctatcattt
     4861 ctattaaaac ggtcataatt tgatgtatgt tatccgtgga taacttaact gctccattta
     4921 acttctcatc atgaatgaag tctcttattt cctccaactg ctggtcctct aatttttcaa
     4981 gcaaatcata cttatcataa taatgcgtat aaaatgtact acggttaaca tcagctaaat
     5041 ctgcaatttg ttgcacagta atctcttcta attggtgttg atgtaaaagt tcaataaatg
     5101 catttctcat tgcaacttgt gattttctaa tacgtcgatc tatagtcatt tgtatcaagt
     5161 cctccccaat gattataaac aattataaat gttattttca ttatcctaca aatctccaac
     5221 attgatgatt ggcacacaat gtttacctgt ttaatatagg tgatacaaac aaacagaaaa
     5281 aggtgataac aatgaaccaa catttactag gaaatccaaa attaactgta actcatgtca
     5341 atgaagtgaa agccggtatt aaccacatcg ttgtcgacag tgttcaatat ggaaatcaag
     5401 aaatgattat ggaaaaagat gtcactgtgg aaatgcgcga tggcgaaaaa ttatatatta
     5461 atattttcag accaaataaa gatggcaaat tccctgtagt tatgtctgca gatacttatg
     5521 gtaaagataa taagcctaaa atcaccaata tgggtgccct ttggccaaca ttaggcgcca
     5581 ttccgacatc tagttttaca cctgaagaat caccagaccc aggattttgg gtgccaaatg
     5641 attatgttgt agttaaagtt gcattacgcg gtagtgacaa atcaaaaggc gtcttatctc
     5701 catggtcaaa aagagaagcg gaagattatt acgaagtgat tgaatgggca gcaaatcagt
     5761 catggagtaa tggaaatatc gggacaaatg gtgtttctta tcttgcggtg actcaatggt
     5821 gggtcgcatc attaaatcca ccacatttaa aagcaatgat tccttgggaa ggcttaaatg
     5881 atatgtatag agaagtagcc tttcatggag gtataccgga tactggcttt tatcgtttct
     5941 ggactcaagg tatttttgcg agatggacag ataatccaaa tatcgaagac ttgattcaag
     6001 cacaacaaga acaccctttg ttcgatgatt tttggaaaca gcatcaagtg caattatcac
     6061 aaattaaaac acctctacta acatgtgcta gttggtctac acaaggtttg cacaaccgtg
     6121 gctcttttga aggatttaaa caagctgtat cagaagaaaa atggctatat gtgcatggac
     6181 gtaaagagtg ggaaagttac tacgctagag aaaatctcga acgccaaaaa tcattctttg
     6241 atttttacct taaagaagaa aataacgatt ggaaagatac gcctcatgtc atttatgaag
     6301 ttagagatca attttataaa ggcgaattca aatcagcgtc agcgttccct ttacctaaca
     6361 cagaatatac accattgtat ttgaatgctg aaaatcacac attgaatcat gcaaagatta
     6421 gtagcgcgta tgtcgcacaa tatgactctg aagataaacg acaagatgta agttttaaat
     6481 atacgtttga caaagatact gagttagttg gaaacatgaa cttaaaacta tgggtaagca
     6541 ctaaagactc agacgatatg gatttatttg caggtattaa aaagttagat cgtcgtggta
     6601 atgaagttaa cttccctgat tttaatcata ttgaaaatgg tcaagtagct accggttggt
     6661 tacgcgtatc acatcgtgaa ttagatcaag aaaaatccac aattgcgcaa ccttggcata
     6721 aacatgaaac agaattaaag ttgtcacaag atgagattgt acctgttgaa atcgaattgt
     6781 taccttcagg cacgctattt aaacaaggcg aaacattgga agttgttgta aaaggtagtg
     6841 aaattgtaat tggtaatagt acccctggca tgaaaacgcg ttatgaacat gaagaaaccg
     6901 taaataaagg catgcacatg atttatactg gtggtaaata tgattcacaa ttaatcattc
     6961 ctatcgttaa ttgatatgca gcaattacgg tcgcttttga ttaaaagtga catagtgata
     7021 gaagagaagg ccacacgctt ggaatcttaa accaaggtgt ggcccttttt attattgatg
     7081 gctatttaat tttataaatc ttgtcactaa acttactaca agcaatatta aaccaataaa
     7141 taaagccatt attgctgtat tactttcgtt gtttactcct gtttttggca attctttcaa
     7201 ttgttgctta tgtactgact tgctcttgct atccaaatga atactttgtt gtttagccat
     7261 ttgtttatta ctttttgtgt gaatatcatt tgctttaata cttggattta cagcactatt
     7321 attcgatgtt ccatttgcgt ttttcaactg attaataaca tttggatctt tcgtgccatc
     7381 tattaaatta tcaagttttt tcacacgtgc tatgtcacta actggcccag gataatttgc
     7441 atccggttgt ctaactggta ttaaattgct gttttcattc acaccttcag gcttgttatt
     7501 ttcggcgttc gcattatccg taaaaatcaa cactgataaa attgcaacga taataccttt
     7561 acttctatta cgctaaccca attggcataa aaataaaaac ccctaatgga taactacatt
     7621 gttatctact agaggtttct aacttatact attttataac actatcgtat tttctttttc
     7681 atgaatcatt tcaataatga cattgtcttc attcattact gctactttag gtgcatggtt
     7741 ttgaatttct tcttcattca actgtgcata agtcatgatt atgactacat cgcctacttc
     7801 aacaagtctt gatgctgcac cgtttaaaca aattttacca ctacctcttt caccagctat
     7861 tacgtatgtt tcaaaacgtg caccattatt attattcacg atggctactt tttcatttgg
     7921 caagatgtct accgcttcca atatatctga atcaatcgta atgctaccta cataatttaa
     7981 atttgactca gtcactcttg ctctatgaat tttagcattc atcattgttc ttatcacttt
     8041 attcagctcc aattattata ttatctatta aacgcgcttt tgaaaattta acagctaacg
     8101 agataaatat gcgtccagtt atttcgtgtt gttctactaa ttgaggataa ctataaacag
     8161 caacttcttc aatgcgtcca cttatatgtg attcaagata ttcagtaacc ctgtctataa
     8221 ttactttact ttgacgttca ccgtcttgat acaacgcttg tgctaatagc aaacttttac
     8281 ttaaatgtac cgcttcttgt cgttcttgct ctgttaaata aacatttctt gaacttttcg
     8341 ccaaaccatc tgcttctcga acgatatcaa taccaataat ttcaacggca tgattgaagt
     8401 cttttaccat ttgctcgaca atagccaatt gctgggcatc ttttttacca aaataagcat
     8461 aatccggcat aacaatatta aatagcttat taactactgt taccacccca tcaaaatgcc
     8521 ctggacgctt cgctccttct aacacatcag ctaatggacc tactttgaca tcaataccta
     8581 attcacctgg atacatatct tctactgcag gatgaaaaac aatgtccgcc cctacttccg
     8641 atactaattc taaatcttta tcaatttgtc tcggataagc atcgaaatct tcgtttggac
     8701 caaattgtaa tggattaaca aatacactca caactgtaat atcatttgta ctaactgatt
     8761 cgcgtaccat cgttaaatgt ccatcatgta aggcacccat tgttgggata aaaccaatcg
     8821 ttgttcctga gcgtttggct gctttaacaa tgtgttgcat ctcttttacc gtagtaatca
     8881 gcttagtcat tgttattaac ctcattcata attttctttt tatacgtata ttcttctgat
     8941 ggaaatgcac cagatttaac ttcttgatcg tattgtttta aaccatccac accaacacta
     9001 aaatcagcaa attgcttcac aaatttcgct ttatgttcaa caccataatt taacatatcg
     9061 tgataaacca atacttgacc atctgtacct tttcctgcac caataccaat gactggaatt
     9121 gttaagtgct tgctaatttc ttctgctaaa tcatttggaa ttgcttcaag tactaacgca
     9181 actgcaccag cttgttctac atttttcgca tctaaaataa gttgctccgc tgcttctttc
     9241 gttgcacctt gtaatttata ccccataacg ccaacacttt gaggtgttaa tcctaaatgt
     9301 gcaacaacag gaataccaat tgccgttgct ttttcaataa atggtgtaat atgcgctcct
     9361 tctgctttaa ttgcatttgc attcgtctcc tgataaagct ttagagcatg atttaagtct
     9421 tgtgtcatag agatgcctac tgcaccaatc ggcatatcaa caactacaaa tgtatttggt
     9481 gcgcctcttc ttactgcacg accgtgatga atcatatctg ctaacgtcac ttgtacggta
     9541 ctttcataac ctaatacagt cataccaagt gaatccccaa caagaatcat atcaataccc
     9601 gctgcttcca cttgtttagc acttggaaaa tcataagctg ttaccataga aattttagtt
     9661 tgcttttgtt tcatatctat taattgactt actgttttca atgttattca acctcttttt
     9721 gcagtattat tagaaatagt ataatgaatc ttttatagaa aggaaagaag aagatgttat
     9781 cagttgcgat tatcggccca ggcgctgtcg gtacaactat tgcctatgaa ttacaacaat
     9841 cattgcctca tacaacgctt atcgggagac acgccaaaac aataacatat tatactgtac
     9901 cacatgcacc tgcacaagat attgttgtga aaggttatga agatgtcaca aacacatttg
     9961 atgttattat tattgcagtc aaaacacatc aacttgatgc agttattcct catttaactc
    10021 atcttgcaca tgaggacacg ctcatcattt tagcccaaaa tggttatggt caactcgaac
    10081 atatttcatt taaaaacgta tgccaagcag ttgtctatat aagtggtcaa aagaaaggcg
    10141 atgttgttac gcacttcaga gattatcaat tacgtataca agataatgca ttaactcgac
    10201 aatttagaga tttggtccaa gacagtcaaa tcgacattgt attagaagca aatattcaac
    10261 aagctatttg gtataaattg ttagttaact tgggcattaa ttctatcaca gcactcggta
    10321 gacagacagt tgcaatcatg cataatcctg aaatacgtac actgtgtaga cagctattac
    10381 tagatggttg tcgtgttgca caggcagagg ggttaaactt ttcagaacaa actgtcgata
    10441 ctattatgac tatatatcaa ggttatcccg acgaaatggg aacgagcatg tattacgaca
    10501 ttgtgcatca gcaacctctt gaagtcgaag caattcaagg ctttatttat cgacgtgcac
    10561 gtgaacacaa tcttgacacc ccatatcttg atactattta tagcttttta cgcgcgtatc
    10621 aacaaaatat gtaagctcat atataatata aaaagacctt aacgatgcat ttagaaccac
    10681 ctgttcctta tatcgctaag gtcttttaat tatattagac tattcagctt ctctaatttc
    10741 ttctgccaca tctttatagt ctattttcgc tttaacaaac gtctcgttat ttaccgggaa
    10801 atgttgttgg aatgtttcaa agttttgtat ctcaacgaca acgtcatcca cttcaaagtc
    10861 aagaacatgt ccaccataag ctctttcatc atctgcaaaa tgtatatgaa aaccagcaga
    10921 tcctacgcca tgaaataatt ctggtgtaaa aaatccaaca atggcaccac gaatattttg
    10981 tcttttttcc tcaggttgtc tgcgtgctga atcaatcaaa cgtgtatatg gcggttgctg
    11041 agcaggcatc attcgtacat gcatatgttt aaatgtgcca taaattttaa cagccgaaaa
    11101 taaattctca cttaacattt catttttaat ttgtgcgaat acatcatctt gtgataattg
    11161 ttgcaatgaa aatgtcttac tcgctttaaa attagtaatc gatgcatacg gtactttctc
    11221 atcgcctttt aattctataa attctttatg ctcgttagca tgatatgcct tcccgtctaa
    11281 aaatattact tcgccatcag accctgttaa cgttgcaatc cctaaattcc catgttctaa
    11341 caattcatta attgtagctg tgccttctag taagccagcc attaacgtac ctaatgtacc
    11401 atgttgatac aagacattcg tcattccaaa accatctccc tatagttatt cctcgttgtc
    11461 atgctctact tcgtgattaa tgcagaacta ctttccttta tactcaatga cgcctcaaca
    11521 tttttattca tgtcactaaa ttttcacgag tcataatact ttattcaatt attattttac
    11581 gcaaaagtat tgcctctatc aatgcttgct aaaaaaatgg acctatcagt gccacgtgtt
    11641 actgcaacac taatagatcc tcttaccatt aatatatttc taattaatac ttaaaatagg
    11701 cttagtcttc taataaatat ttaattgaat caaatgtatc ttctaatgtt ttaacagatt
    11761 tatcgaacaa cgcttgttct tcggcactta atggcatttc ataaatttta actgcgccat
    11821 gttggttaac taatgttggt acacctaggt aaacgccttt gtgaccacca tattgaccat
    11881 ctaattgtat agaaacattt aagacattat tttcattatt taaaatggct tttgaaatgc
    11941 gcatcaatgc taatgcaata ccatagtatg ttgacccttt agcttggata atttcataag
    12001 cagcgtcacg tgtatttaca taaatttctt ccgctttagc ttcgctacca gtttgttctt
    12061 ttaatgtgtc atatactgaa atacctgcta catttgcttg tgaccaaact gcaagttcag
    12121 tatcaccatg ctcaccaata atactagcgt caacacttga aggtgcaaca ccaagttctt
    12181 ggctaattaa atattgtaaa cgtgcactgt ccaatacagt acctgaaccg ataacacgct
    12241 ctgctggtaa tccagtatat tcttttacaa atcttgttaa aatgtctaca gggtttgccg
    12301 cgattaagaa atatccatca aagccactat ccataacact cttaacgatg ctcttcataa
    12361 tcttagtatt tttttcaact aattgtaaac gtgtttcacc tggcttttga ggtgcaccag
    12421 ctgtaataac aactaaatct gcatctttac agtcttcgta ttcacctgct ttcacatcaa
    12481 ctggtgaagg actgtggact gtaccatggt ttaaatcttg aacatctgct tttactttgt
    12541 cttttgcaat gtcaataatt acaaattcat cagcaacacc ttgcgtaacc attgcaaagg
    12601 catagcttga tcctacagat ccatctccga ttaatacaac ctttttacca aatgttttca
    12661 taattatgtc tcctaaccta attgaattta ttttacacgt gactacacgt aaaaatatct
    12721 atactgaaat taaagattaa
//
LOCUS       DBNENW010000069        12655 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNENW010000069 DBNENW010000000
VERSION     DBNENW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.069"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            64..2160
                     /gene="pnp"
                     /locus_tag="AC1LW8_001564"
     CDS             64..2160
                     /gene="pnp"
                     /locus_tag="AC1LW8_001564"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIP4773395.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPVERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2396..4069
                     /gene="rnjB"
                     /locus_tag="AC1LW8_001565"
     CDS             2396..4069
                     /gene="rnjB"
                     /locus_tag="AC1LW8_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J2"
                     /protein_id="HIP4773396.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4326..6695
                     /locus_tag="AC1LW8_001566"
     CDS             4326..6695
                     /locus_tag="AC1LW8_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIP4773397.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVVLENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6700..7413
                     /locus_tag="AC1LW8_001567"
     CDS             6700..7413
                     /locus_tag="AC1LW8_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP4773398.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7444..8709
                     /gene="yfmF"
                     /locus_tag="AC1LW8_001568"
     CDS             7444..8709
                     /gene="yfmF"
                     /locus_tag="AC1LW8_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIP4773399.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTTTARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8709..9995
                     /gene="yfmH"
                     /locus_tag="AC1LW8_001569"
     CDS             8709..9995
                     /gene="yfmH"
                     /locus_tag="AC1LW8_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIP4773400.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFVIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVNEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            9995..10699
                     /gene="ymfI"
                     /locus_tag="AC1LW8_001570"
     CDS             9995..10699
                     /gene="ymfI"
                     /locus_tag="AC1LW8_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P 5-aminopentanone reductase"
                     /protein_id="HIP4773401.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLILPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10804..11631
                     /locus_tag="AC1LW8_001571"
     CDS             10804..11631
                     /locus_tag="AC1LW8_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HIP4773402.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11650..12042
                     /locus_tag="AC1LW8_001572"
     CDS             11650..12042
                     /locus_tag="AC1LW8_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773403.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12076..12654
                     /gene="pgsA"
                     /locus_tag="AC1LW8_001573"
     CDS             12076..12654
                     /gene="pgsA"
                     /locus_tag="AC1LW8_001573"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIP4773404.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
BASE COUNT         4618 a         1699 c         2454 g         3884 t
ORIGIN      
        1 aatattaaaa tacgataata aatgatatga tataactatt agattcaaga gaggagattt
       61 ataatgtctc aagaaaagaa agtttttaaa actgaatggg caggaagatc tttaacgatt
      121 gaaacagggc aattagctaa acaagcaaat ggcgctgtat tggttcgtta tggagataca
      181 gtcgtgttat cgacggcaac tgcatcaaaa gaacctcgtg atggagattt cttcccatta
      241 acagtgaact atgaagaaaa aatgtacgct gcgggtaaaa ttcctggtgg atttaaaaag
      301 agagaaggac gtcctggtga cgatgcaaca ttaactgcgc gattaattga tagaccaatt
      361 agacctttat tccctaaagg atataagcat gatgttcaaa ttatgaacat ggtattaagt
      421 gcagatcctg attgttcacc acaaatggct gcaatgattg gttcatctat ggcgcttagt
      481 gtgtcggata ttccattcca agggccaatc gccggtgtaa atgtgggtta tattgacggt
      541 aaatatatca ttaacccaac agtagaagaa aaagaagttt ctcgcttaga ccttgaagta
      601 gctggtcata aagatgcagt aaacatggta gaggcaggcg ctagtgagat tactgaacaa
      661 gaaatgttag aggcgatttt ctttggtcat gaagagattc aacgtttagt tgatttccaa
      721 caacaaatcg tcgaccacat tcaacctgtt aaacaagaat ttattccagt agagcgtgat
      781 gaagcgctag ttgaacgtgt aaaatcttta accgaagaaa agggacttaa agaaacagtt
      841 ttaacatttg ataaacaaca acgagatgaa aatcttgata acttaaaaga agaaatcgtc
      901 aatgaattta tcgatgaaga agatccagaa aatgaattac ttattaaaga agtttatgca
      961 attttaaatg aattagtgaa agaagaagtt cgacgtttaa ttgcagatga aaaaattaga
     1021 ccagacggcc gtaaacctga tgaaatccgt ccattagatt ctgaagttgg tattttacct
     1081 agaacgcatg gttcaggtct atttacacgt ggtcagactc aagcactttc agttttaaca
     1141 ttaggtgctt taggcgatta tcaattaatt gatggtttag gacctgaaga agaaaaaaga
     1201 ttcatgcatc attacaactt cccgaatttt tcagtaggtg aaactggtcc agtacgtgcg
     1261 ccaggtcgtc gtgaaattgg acatggtgcg ttaggtgaaa gagcattaaa atatattatt
     1321 cctgatactg ctgatttccc atatacaatt cgtattgtaa gtgaggtact tgaatcaaat
     1381 ggttcatcat ctcaagcgtc aatttgtgga tcaacattag cattaatgga tgcgggcgta
     1441 ccgattaaag caccagttgc tggtattgct atgggccttg ttacacgtga agatagctat
     1501 acgattttaa ctgatatcca aggtatggaa gatgcattag gtgatatgga ctttaaagtc
     1561 gctggtacta aagaaggtat tacagcaatc caaatggata ttaaaattga cggtttaacg
     1621 cgtgaaatta tcgaagaggc tctagaacaa gcgagacgtg gtcgtttaga aataatgaat
     1681 catatgttac aaacaattga tcaaccacgt actgaattaa gtgcttacgc gccaaaagtt
     1741 gtaactatga caattaaacc agataagatt agagatgtta tcggacctgg tggtaaaaaa
     1801 attaacgaaa ttattgatga aacaggcgtt aaattagata ttgaacaaga tggtactatc
     1861 tttattggtg ccgttgatca agctatgata aatcgcgctc gtgaaatcat tgaggaaatt
     1921 acacgtgaag cggaagtagg tcaaacttat caagccactg ttaaacgtat tgaaaaatac
     1981 ggtgcgtttg taggcctatt cccgggtaaa gatgcgttgc ttcacatttc acaaatttca
     2041 aaaaatagaa ttgaaaaagt ggaagatgta ttaaaaatcg gtgacacaat tgaagttaag
     2101 attactgaaa ttgataaaca aggtcgagta aatgcttcac acagagcatt agaagaataa
     2161 tatttaaagt catatgacga caatgtatcg tcatgtgatt tttttatgcc attttttacg
     2221 aagtgatcca atttgaattt gttgtattga acattttaaa acgctttatt attttgtgtg
     2281 caactgttaa ttatcctgta tgtatagtga ttaatagtgt acatcaagtg ttttttaact
     2341 tataatgaat agtgagttta tatatggacg ggtaacaaat ttaggaggta agattttgag
     2401 tttaataaag aaaaagaata aagatattcg cattatacca ttaggcggtg ttggcgaaat
     2461 tgctaaaaat atgtatatcg ttgaagtaga cgatgaaatg tttatgttag atgctggact
     2521 tatgtttcca gaagacgaaa tgctaggtat tgatattgtt ataccagaca tttcatacgt
     2581 acttgaaaat aaagataaat tgaagggtat attccttaca cacggacatg agcacgcgat
     2641 tggtgcagtg agttatgttt tagaacaatt agatgcacca gtatatggat ctaaattgac
     2701 aatagcgtta attaaagaaa atatgaaagc ccgtaatatt gataaaaaag ttcgctacta
     2761 cacagttaat aatgattcaa ttatgagatt caaaaacgtg aatattagtt tctttaatac
     2821 gacacacagt attcctgata gtctaggtgt ttgtattcac acttcatatg gtgccattgt
     2881 gtatacaggt gaatttaagt ttgaccaaag tttacatgga cattatgcac cagatattaa
     2941 acgtatggca gagattggtg aagaaggtgt atttgtctta atcagtgatt ctactgaggc
     3001 agagaaacct ggatataata ctccggaaaa tgtgattgaa catcatatgt atgacgcttt
     3061 tgcaaaagtg cgaggtcgct tgattgtttc atgttatgct tcgaacttta tacgtataca
     3121 gcaagtttta aatattgcta gcaagctaaa tcgtaaagtg tcatttttag gaagatcact
     3181 tgaaagttca tttaatattg ctcgtaaaat ggggtatttc gacattccta aagatttgct
     3241 aattcctata acagaagttg ataattatcc taaaaatgaa gtgataatta tagctactgg
     3301 tatgcaagga gaacctgtag aagccttaag tcaaatggcg caacataagc ataaaattat
     3361 gaatatcgaa gaaggcgatt ctgtattttt agcaattacg gcttctgcaa atatggaagt
     3421 tatcattgcg aatacattaa atgagcttgt acgtgctggc gcacatatta ttccaaataa
     3481 caagaagatt catgcttcaa gtcatggttg catggaagaa ttaaaaatga tgattaatat
     3541 tatgaaacct gaatacttta ttcctgtaca aggtgaattt aaaatgcaga tagcacatgc
     3601 gaagctagca gctgaagcag gtgttgcacc agaaaagatt ttccttgtgg aaaaaggaga
     3661 tgtcattaat tacaacggta aagatatgat attaaatgaa aaggtaaatt caggaaatat
     3721 tttaatagat ggtattggta ttggggatgt aggaaatatc gtgttgagag accgtcatct
     3781 tttagcagaa gatggtatct ttattgctgt agtaacgtta gatcctaaaa atagacgtat
     3841 agctgcggga cctgaaattc aatctcgtgg gtttgtatat gtacgtgaaa gtgaagactt
     3901 attacgtgaa gcagaagaga aagtacgtga aatagtagag gctggtttac aagaaaaacg
     3961 catagaatgg tctgaaatta agcaaaatat gcgtgatcaa attagtaaac tattattcga
     4021 aagtacaaaa cgtcgtccta tgattattcc agtaatttct gaaatttaat caaaaagtca
     4081 ttaacataaa agaggtcaga acaagtcact gaaatataat ggttgtcatg gacaatttac
     4141 ttatatttta tgatggtcaa ttgaaagggg taacgattaa tctgttatct taagtaaatt
     4201 gatacataga tgatattgtt ctaacctctt tcatcgtctg tttggactac atattctaaa
     4261 catcaaatag gaaattatat ataataacgt cgttttaact aaggcaacat aaggaggtgc
     4321 gtcaattggc acaagcaaaa aagaaatcga cagctaagaa aaaaacaaca tcaaaaaaaa
     4381 gaacaaattc gaggaaaaag aagaatgata atccgatacg ttatgtcata gctattttag
     4441 tagttgtatt aatggtgttg ggtgttttcc aattaggaat aataggtcgt ctaattgaca
     4501 gcttctttaa ttatttattt gggtacagta gatatttaac atatatttta gtactcttag
     4561 caactggttt tattacatac tctaaacgta ttcctaaaac tagacgaacg gctggttcga
     4621 ttgtattgca aattgcattg ctatttgtat cacagttagt ttttcatttt aatagtggta
     4681 tcaaagctga aagagaacct gtactttctt atgtgtatca gtcataccaa cacagtcatt
     4741 tcccaaattt tggtggcggt gtattaggct tttatttatt agagttaagc gtacctttaa
     4801 tttcattatt tggtgtatgt attattacta ttttattatt atgctcaagt gttattttat
     4861 taacaaacca tcaacatcgt gaagtcgcaa aagttgtact ggaaaatata aaagcttggt
     4921 ttggttcatt taatgaaaaa atgtcggaaa gaaaccaaga aaaacaattg aagcgtgaag
     4981 aaaaagcgag acttaaagaa gaacaaaagg cacgtcaaaa tgaacagcca caaataaaag
     5041 atgtgagtga ttttacggaa gtgcctcaag aaagagatat tccaatttat gggcatactg
     5101 aaaatgaaag taaaagtcag agtcaaccaa gtcggaaaaa gcgagtgttt gatgcagaga
     5161 atagttcgaa taacatcgta aatcatcatc aagcagatca gcaagaacaa ttaacagaac
     5221 aaactcataa cagtgttgaa agtgaaaaca ctattgaaga agctggtgaa gttacgaatg
     5281 tatcgtatgt tgttccaccg ttaactttac ttaatcaacc tgcaaaacaa aaagcaacat
     5341 ctaaagctga agtgcaacgt aaaggacaag tactagagaa tacattaaaa gattttgggg
     5401 taaatgcaaa agtgacacaa attaaaattg gtcctgcagt aactcaatat gaaattcaac
     5461 cagctcaagg ggttaaagtg agtaaaattg taaacttgca taatgatatt gcattagctt
     5521 tagcagcaaa agatgttaga atcgaagcgc caatacctgg tcgctctgca gtaggtattg
     5581 aagtgccaaa tgagaaaatt tcattagttt cactaaaaga agttttagat gaaaaattcc
     5641 cgtctaataa taaactagaa gttggattag gaagagatat atcaggtgat ccaattactg
     5701 ttccactaaa tgaaatgcca cacttattgg tggcaggatc gacgggtagt ggtaaatctg
     5761 tttgtataaa tggtattatt acaagtattt tattaaatgc taagccgcat gaagttaaac
     5821 ttatgttaat cgatccgaaa atggttgaac taaatgttta taacggaatt ccacatttat
     5881 taattccggt tgttacaaat cctcataaag ctgctcaagc tttagaaaaa attgtagctg
     5941 agatggaaag acgttatgat ttattccaac attcatcaac tagaaatatt aaaggttata
     6001 acgaattaat ccgtaagcaa aatcaagaat tagatgagaa gcaaccagaa ttaccttata
     6061 tcgttgttat tgtagatgag cttgcagatt taatgatggt agctggtaaa gaagttgaaa
     6121 atgcgattca acgtattaca caaatggcac gtgcagcagg tatacattta attgtagcga
     6181 cacaaagacc ttctgtggat gtaattacag gtatcattaa aaataatatt ccatctagaa
     6241 ttgcttttgc tgtaagttct caaacagatt caagaactat tattggtact ggcggcgcag
     6301 aaaagttact tggtaaaggt gacatgttat acgttggaaa tggtgattca tcacaaacac
     6361 gtattcaagg ggcgttttta agtgaccaag aggtgcaaga tgttgtaaat tatgtagtag
     6421 aacaacaaca ggcaaattat gtaaaagaaa tggaaccaga tgcaccagtg gataaatcgg
     6481 aaatgaaaag tgaagatgct ttatatgatg aagcgtattt gtttgttgtt gaacaacaaa
     6541 aggcaagtac atcattgtta caacgccaat ttagaattgg ttataataga gcatctaggt
     6601 tgatggatga tttagaacgc aatcaggtaa tcggtccaca aaaaggaagc aagcctagac
     6661 aagttttaat agatcttaat aatgacgagg tgtaaaaaaa tgtcagaaat gaatgcggta
     6721 tataacgtta aacaatacat tttaaatttg attaagcaaa ataaattgga atatggtgac
     6781 caacttccaa gtaatttatc aattgccaga gaattaaatg taaaaaccga cgatgtttat
     6841 gaagcaattc aggcattgat tactgaacaa gtcattaaag ataattttga agagggcaca
     6901 agtgttaagt cactgccccc tttcttttat ccattgaatg aacttataag tattgggcaa
     6961 atgattaaaa atgcaggatt tgaatgcgga actgaatact taaattttga tgagcaacca
     7021 gcaactatgt tagatgcaaa tttgttgagc gttgaagaag gatatccagt aaccattata
     7081 gaacgattac gaactgccga tggagaaccg gtcgtctatt gtttagataa aattgctaaa
     7141 aaagaattaa catgtacaga gtatcaaatg agcaatggat cgatactaag tgcgataaaa
     7201 gaacaaagta atcataatat ttgttacgct gatacagaaa ttgaagcggt aaattatgaa
     7261 cctcgaatat ccgaagtact gaatgcttcg ccacacgaag gtttgatttt attaaaaatt
     7321 acgcactaca atgaattaga tgaacctatt ttgtattcat taaattatat gaaaaatagc
     7381 ttagttcaat ttaaaatcac tagaaaaata tagatgtaag aagatataga ggaggctata
     7441 tatttgagta gacaatctca accaaatata catatcaaag tttcaccaac aactaaattt
     7501 aaaacaacta ctatagtttt taaatttatg gcacctttag aatatgacac aactacagct
     7561 agatcattat taagcaaatt attagttcga gcaactaaga aatggccaac cgataagtcg
     7621 tttaataatc acttagccga tttatatggt gcatatgtga atagtacaat ttcaaaattc
     7681 aaagatcagc atgtcattac attttcatta gaaattgtaa atgaacgtta tttaagaaac
     7741 ggtgaatcat tatttaatca aggattagat ttattacaag aaatcatttg gaatccatta
     7801 attgaaaata aagcattcaa tgataatttt gttaaccaag agaaaacatt attagccaaa
     7861 aaaatagaag caatggtaga taacaaagca caatattcgt ttttaaaatt actcgaccat
     7921 atgtttgaaa atgaagcata caaatactta tctacaggac aactagaaca aatcccacat
     7981 attactgctg aaacactata tcatacatat caatcaatga ttaataatga tcaatgttct
     8041 gtttatgttg tcggcaatgt agaacctgaa agtgttgaga aacaaatacg tgaaaaattt
     8101 gcacttaaac cattcgataa acatcaattc caacattcta cacatcattt acacgatgaa
     8161 gaagttgatt atattgttga atatgatgac gtggatcaag ctaaattaaa tatgggatat
     8221 cgttttccaa cacaatatgg acaaagtgga tatgctgctt ttgttgtatt taacatgatg
     8281 tttggaggag atccttcatc tgttttattt aatgaagtgc gagaaaagca aagtttagcg
     8341 tactctatac attcacaaat tgatggcaaa aatggctatt tatttgtttt gagtggggtt
     8401 tcaagtgata agtacgaaac tgcaaaagac actattataa gtgaatttga aaaaataaaa
     8461 gcaggagatt tcactgaaga aaaattagag ttagctaaaa aagtaatcat ttctcatcga
     8521 tatgaatctg aagatcgtcc gaaaagtatt atagagatta tgcataacca aatattatta
     8581 gagcaaccac aaagcaaaga aacatttata aatgatatac agaaggtaag tcgcgaagat
     8641 attgtttctg ttgctgaaaa agcattttta gatacaatct atgtgttgac aaaaggaggg
     8701 gataaataat gaaagagcgt tattatgaat taatagacga aagagtattc gaacaagaat
     8761 tagaaaatgg tttacgatta tttgttatcc ctaaaccagg ttttcaaaag acatttgtca
     8821 cttacactac acaatttggt tcattagata atcaattcaa acccctcgga caagaccaat
     8881 ttgttactgt acctgatgga gttgctcact ttttagaaca taaattattt gaaaaagaag
     8941 aagaagactt atttactgca tttgctgaag ataatgcaca agcaaatgcg tttacaagct
     9001 ttgatcgtac aagctacttg ttcagtgcaa ctgataatat tgaaaacaac attaaacgtt
     9061 tacttacaat ggttgaaacg ccttatttta caaaagaaac tgttgataaa gaaaaaggta
     9121 ttattgcaga agaaataaaa atgtatcaag agcaacctgg atataaatta atgtttaata
     9181 cattgcgtgc aatgtatcaa caacatccaa tacgtgttga tattgccggt agtgtagaaa
     9241 gtatatacga tattacaaaa gatgatttgt atctatgtta tgaaacgttt tatcatccat
     9301 caaatatggt tttatttgtt gttggcgatg tggatcctga agcaatatgt cgaatagtaa
     9361 aacaacacga ggatgctcgt aataaagtta accaacccaa aatcgaacga ggacttgtta
     9421 atgaaccgga ggatgttaaa gaagcatttg ttactgaatc tatgaaaatt caatcaccaa
     9481 gactaatgct tggttttaaa aataaaccat tacaagaagc gcctcaaaaa tatgtacaac
     9541 gtgatttaga aatgtcatta ttctttgagt taatttttgg ggaagaaaca gatttttatc
     9601 agaatttatt aaacgaagga cttatcgatg atacatttgg ttatcaattt gtactagagc
     9661 cgacttatag tttttcaatc gtgacaagcg ctactgaaga accagataaa ttaaaaaaat
     9721 tattattaga tgagttgcgt gataaaaaag gcaatttcca agatgcagaa gcatttgaac
     9781 ttttgaaaaa gcaatttata ggtgaattca tatcaagttt aaactcacct gaatatattg
     9841 ctaatcaata tactaaattg tattttgaag gtgttagcgt gtttgacatg ttagacattg
     9901 ttgaaaatat caccttagat agtattaacg aaacgtcatc gttatattta aatttagatc
     9961 agcaagtcga tagtcgtttg gagattaaaa agtaatgaaa gcattagtat taggtggttc
    10021 tggttcaatt ggttctgaga tagtcaaaca attattaact gatggatttg aagtttatgt
    10081 gcaatattat cgtactgata taaatgaatt aactagcaaa tttaatgatg ataaagttcg
    10141 ttttatacaa gcggatttat ctcaaacaat tgatattgac aaaacatttg gtgacattaa
    10201 atcattagac tgtttaatat atgcaagtgg tcagtcttta tatggtgttt tacaagatat
    10261 gaaagaccat gatattgatg catgttatca gttaaatgtc ttgcaattaa ttcgattatg
    10321 tagatatttc gttgatgttt tacgtcaaag tgacaatgga agaattattg taatttcatc
    10381 aatttggggt gagacaggag ctagtatgga aactatttat tcgacgatga aaagtgcaca
    10441 attaggtttc gttaaggcgc ttagtcaaga gcttgcacta acatcagtga cagtaaatgc
    10501 tatcgcacct ggatttgtag ccggtaatat ggcaagtgag tggcaagaag atgaacttca
    10561 agcaatgata actgaattac cacaacagcg attgatttta ccgagtgagg ttgctcatac
    10621 atgcgcctat ttatatcacc caaatgctag aagtgttact ggaactatac agaaagttaa
    10681 tggtgcttgg tatatttaaa atatagaact atttttatga gcaaatgttt ttatgattga
    10741 tgttataata tgaatattac aatgaatcat aatttgttaa ttaaacaagg ggtgaggttc
    10801 accatgacag ttgcagagaa aaaagaatgg tacctagaat acgaaattga aattaataga
    10861 ccgggtcttt taggtgatgt atctagttta ttaggtatgt taggtataag tattgttaca
    10921 attaatggtg ttgatcaagg taaacgaggc cttttaatta aaacagacaa tcttgaaaaa
    10981 gttgaacgtt ttgagcaaat agctcgtggt ataaatgaaa ttgaaataac aaagcttaaa
    11041 aaaccagaat taagagaccg tcttgcagta agacatggta gatatattga gcaagatgca
    11101 aaagataaga aaacctttcg atttgagcgt gaagatttag gcttgttagt agacttttta
    11161 gctgaattgt tcaaagaaga aggtcataag ttgattggca ttagaggtat gccacgagtt
    11221 ggtaaaactg aatcaattgt tgcgggaagt gtttgtgcac ataagagatg gttatttatt
    11281 agttctactt taataaaaca aactgtacgt agctctctaa ttaaagggga atatgatgcc
    11341 aatcatgtat acattattga tggtgcagtt actgccagag aatctaatcc aaaacatcaa
    11401 gagcttgtta acgaagttat gacgttacca tcaatcaaag tcgttgaaca tccagattta
    11461 tttgttgaaa caagtacttg tacaatggaa gattttgact atattatcga attgagagaa
    11521 aatgaaaatc aagaaataca ttacgaagaa atgaagaaac agacagtcca aagtaagaat
    11581 aatttagatt ttggagatcc gtttggtggt ggttttggtt tcttcgagta agtgtaagga
    11641 ggctatgaat tgaaaacggt cggtgaagcg ctaaaaggta gacgtgaaag gttaggaatg
    11701 actttaacag aattagagca acgtactgga attaaacgtg aaatgctagt gcatattgaa
    11761 aataatgaat tcgatcaact accgaataaa aattacagcg aaggatttat tagaaaatat
    11821 gcaagcgtag taaatattga acctaaccaa ttaattcaag ctcatcaaga tgaaattcca
    11881 tcgaaccaag ccgaatggga cgaagtaatt acagttttca ataataataa agacttagat
    11941 tataagagta aatcaaaaga gccaatacaa ttattagtaa tcatgggtat tacagtttta
    12001 ataactttat tgttatggat catgttagtt ttaatatttt aacagaaata aattagtgag
    12061 aaatgaggat gtataatgaa tattccgaac cagattacgg tttttagagt agtgttaata
    12121 ccagttttta tattgtttgc gttagttgat tttggatttg gcaatgtgtc atttctagga
    12181 ggatatgaaa taagaattga gttattaatc agtggtttta tttttatatt ggcttccctt
    12241 agcgattttg ttgatggtta tttagctaga aaatggaatt tagttacaaa tatggggaaa
    12301 tttttggatc cattagcgga taaattatta gttgcaagtg ctttaattgt acttgtgcaa
    12361 ctaggactaa caaattctgt agtagcaatc attattattg ccagagaatt tgccgtaact
    12421 ggtttacgtt tactacaaat tgaacaagga ttcgtaagtg cagctggtca attaggtaaa
    12481 attaaaacag cagttactat ggtagcaatt acttggttgt tattaggtga tccattggca
    12541 acattgattg gtttgtcatt aggacaaatt ttattataca ttggcgttat ttttactatc
    12601 ttatctggta ttgaatactt ttataaaggt agagatgttt ttaaacaaaa ataaa
//
LOCUS       DBNENW010000070        12578 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNENW010000070 DBNENW010000000
VERSION     DBNENW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.070"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            150..764
                     /locus_tag="AC1LW8_001574"
     CDS             150..764
                     /locus_tag="AC1LW8_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773405.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQANNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(918..2591)
                     /locus_tag="AC1LW8_001575"
     CDS             complement(918..2591)
                     /locus_tag="AC1LW8_001575"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIP4773406.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPDLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPN
                     SERADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            2908..4506
                     /locus_tag="AC1LW8_001576"
     CDS             2908..4506
                     /locus_tag="AC1LW8_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIP4773407.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            4887..6365
                     /gene="mqo"
                     /locus_tag="AC1LW8_001577"
     CDS             4887..6365
                     /gene="mqo"
                     /locus_tag="AC1LW8_001577"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HIP4773408.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(6431..6886)
                     /locus_tag="AC1LW8_001578"
     CDS             complement(6431..6886)
                     /locus_tag="AC1LW8_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HIP4773409.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVNTINKQLKQLK"
     gene            complement(6883..7326)
                     /locus_tag="AC1LW8_001579"
     CDS             complement(6883..7326)
                     /locus_tag="AC1LW8_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIP4773410.1"
                     /translation="MMKLNLFINAKETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(7489..8862)
                     /gene="hssS"
                     /locus_tag="AC1LW8_001580"
     CDS             complement(7489..8862)
                     /gene="hssS"
                     /locus_tag="AC1LW8_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HIP4773411.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDNTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(8855..9529)
                     /gene="hssR"
                     /locus_tag="AC1LW8_001581"
     CDS             complement(8855..9529)
                     /gene="hssR"
                     /locus_tag="AC1LW8_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HIP4773412.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            9665..10720
                     /locus_tag="AC1LW8_001582"
     CDS             9665..10720
                     /locus_tag="AC1LW8_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773413.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFVITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            10720..11385
                     /locus_tag="AC1LW8_001583"
     CDS             10720..11385
                     /locus_tag="AC1LW8_001583"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773414.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(11616..>12578)
                     /locus_tag="AC1LW8_001584"
     CDS             complement(11616..>12578)
                     /locus_tag="AC1LW8_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIP4773415.1"
                     /translation="LNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYSIM
                     TYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFIST
                     IQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTEFV
                     TPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDILM
                     ESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGSHT
                     PYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT         4349 a         1789 c         2216 g         4224 t
ORIGIN      
        1 aaagaacctt agattgtagt tatggatatt gtgataattc agatcatatt aatgttgacg
       61 tctttggtaa caagccatga aaagatacac atggtagata tgtatttcaa gattattcaa
      121 tgaatatcga attataggag gagatatgta tgaaaagatt agttacaggg ttactagcat
      181 tatcattatt tttagctgca tgtggtcaag atagtgacca acaaaaagac ggtaataaag
      241 aaaaagatga taaagcgaaa actgaacaac aagataaaaa aacaaatgat tcatctaaag
      301 ataagaaaga taataaagat gatagtaaag acgtaaacaa agataataaa gataatagtg
      361 caaacgataa ccagcaacaa tctaattcaa atgcaacaaa caatgaccaa aaccaaacaa
      421 ataataacca agcgaataat aatcaaaaat caagttacgt tgcaccatat tatggacaaa
      481 atgccgcgcc ggttgcacgt caaatttatc cgtttaatgg aaataaaact caagctttac
      541 agcaattgcc aaatttccaa acagctttaa atgcggctaa taatgaagca aataaatttg
      601 gtagtaataa taaagtgtat aatgattatt ctattgaaga acataatggc aactataagt
      661 atgtgtttag ttttaaagac ccaaatgcaa atggaaaata ttcaattgta acggttgatt
      721 atactggaca agcaatggtt actgatccaa actaccaaca ataatgttaa tataactatg
      781 atgcaagtta aaaaaataaa acggtaaact ctataatatg aattagggtt taccgttttt
      841 tgcgtatttt aaagtatcaa agtttacgtt ttatagtact tttaaacctc attcacagcc
      901 actgagaaat ttaaacacta ttgattagat gtcatcttag acaatataag ttgtgaaata
      961 gattctaatg gttgttcttt atgttgtatt tgtggatatt taatcactgg tattgcctgg
     1021 gaaattaaat tattaattaa taattgtcgg tctgtatacc aaatttttga tagatgacgc
     1081 caaatatctt cgttaacacc ttgagactca atgtattgac tgtgatttgg agtgtataat
     1141 gctactgttt tgtctatagc catcgcatca aatattatat ttgagtaatc agtgataacg
     1201 acatcagaca ttagaattaa gtcttgagca ctaagatact ttggtgcaac aagcgcctct
     1261 tctggtaaga tcgcttcatc aacaccctga accacaacat gatatgcttt gaataaagca
     1321 tctgatagtg gtaattgttg cgcactcact aatccaatag gtgcgtataa taaaactggt
     1381 ttttgatcat ttattttaaa cgatttctta tactgttgtt gagtagtact ttcgttacgc
     1441 ttttgtagta aatattggtg tttaggatta ccataagcca atacatttgc ttggtgacta
     1501 ggaaaagccg tttgataaaa gtggctaata tcatcagcag aatgtataac ataatcgaat
     1561 tgcaaccagc gattatacct tttagcacga taaaaaggtg tttctatatt tttaataggc
     1621 tctttactat ctaaaaataa ttgcattatt ggtgtgcctt gatgtaattg aattaaagta
     1681 ccgtttggtt gtaacgtttc aggtatatca ttttcaacta taactacgcg tgcgctgtta
     1741 atcaattcgt cagctctttc agaattaggt gatatgaaat gtggtccgcg acgatcgtca
     1801 gtaacaaaat aacaggcagt cattggatta tgtttaacga agtaagtaaa taaatatttt
     1861 gaattaccta tatattgatg atgatcaccg ataaaaacga tgtgatcttt taaaacgttt
     1921 ggttgcttcg tatacaggcc gaagttttgt aattgtttgt tatcagcacg ttgctttgct
     1981 attttatata accatttagg tagcataaac ttggtagaat acaaccaatt atctggcgaa
     2041 ttaagttgtt ctgaatcctc gataaaaagg ttgcgaaaag gtataggatg atgatcaata
     2101 taagccattt tattcaaata aaactgagac atcatatcaa aatcttcaat agttttaata
     2161 gtttcccata ttgaagcact tgttgctata ggggctttaa aatcaaaaac gacatgacca
     2221 tcgattgata aatcaccttt cagaagtgtc aataaagcta aatcgaaaat cgtttctaca
     2281 gagtaataat gtattaaatc aagagtctgt tgaatcgcat atatcaggtc aggataataa
     2341 ttattgtttt gagcccaatc ggcaatgttg atcgatgatt gaccgaaata gtgacaatca
     2401 tttttgtatt gtccatccac agtataatca accacgatag acttaggata taattgaacc
     2461 gcatctaaca tcgattgata aatcattatc tcatttgcat atggaacgac atgtgtataa
     2521 ccttcagact gagctttcat taattgagat tcaaactttt gcatattaga aacatttatc
     2581 tgtttaatca ttgctataac ttccatttct catcgtagta tcttccttat tttagcacgg
     2641 tcttatgtaa aattcatgaa tagtataagt tacttcatta cattaagtac aattcatttt
     2701 gatgaacaga tttaaaatga aaaataaaac aattaaatag gaaagtgata ttgttaatag
     2761 tctgaaaaaa tggtatattt taattgtgaa aaaaatcaca ttaatctcac tgttctgtaa
     2821 aatgttatcg ataacaaaat gctatagcca gagcatgatt ttaatgaagc aattacacta
     2881 ctactttatg gaaagaagag tcagcctatg ttagtaaata cgtttaaccc atttgataat
     2941 ttattattat cgtctttaat tgcagcaata ccgattgtac tgtttttact atgtttaact
     3001 gtttttaaaa tgaaaggtat ttatgcagct attacaacac ttgttgtaac attactaatt
     3061 gcaataccat ttttcaaatt gccagttggt atcgcttctg gtgcagtagt cgaaggtttc
     3121 ttccaaggta tcattccgat tggctatatc gttatgatgg cagtattgtt atacaaaatt
     3181 actgttgaat ctggacaatt tttaacaatt caagatagta ttacaaatat ttcacaagac
     3241 caacgtattc aagttttact tattggattt gcattcaacg catttttaga aggtgcagca
     3301 ggatttggtg taccaattgc aatttgtgca cttttattaa cacaattagg atttaatcca
     3361 ttaaaagctg cgatgttatg tttagtcgca aatgcagcgt ctggtgcttt tggtgcgatt
     3421 ggtatccctg taggtgttgt agaaacgttg aaattacctg gagatgtttc agtattaggt
     3481 gtttctcaat cagcaacttt aacattggca atcataaatt tcattattcc attcttactt
     3541 atctttatta ttgatggttt tagaggtgtt aaagaaacat taccagcaat tttagtagtt
     3601 tcaatcacat atacacttac tcaaggatta ttaactgtat tcagtggacc tgaattggca
     3661 gatattattc caccgttatt aacaatgtta gcattagcag tattttctaa aaaattccaa
     3721 ccaaaacaca tttatcgtgt taataaagat gaagaaattg aacctgcaaa agcacattct
     3781 gcaaaagcag tattacatgc atggagtcca tttattgtat taacagtcat tgtaatgatt
     3841 tggagtgcgc cattctttaa aaacttattc ttaccaaatg gtgctttatc atcattagta
     3901 tttaaattca acttacctgg aacaatcagc gaagttacgc ataaaccatt agtattgact
     3961 ttaaatatta ttggacaaac aggtacagct attttattaa ctattattat tacaatttta
     4021 atgtctaaaa aggttaactt taaagatgct ggtagattat tcggcgttac atttaaagag
     4081 ttgtggttac cagttcttac aatttgtttc atcttagcaa tttctaaaat cacaacttat
     4141 ggtggtttaa gtgcagcaat gggtcaaggt atagctaaag caggtaatgt cttcccagtt
     4201 ctatcaccaa tcttaggttg gataggtgtg tttatgacag gatcagttgt aaataacaat
     4261 tcattatttg caccaattca agcttctgtt gcacaacaaa ttggaacaag tggttcactt
     4321 cttgtatctg ctaatacagt tggtggtgta gcggcaaaat tgatttcacc acaatcaatt
     4381 gcaattgcaa ctgcagcagt aaaacaagtt ggtaaggaat cagaattatt aaaaatgaca
     4441 ttgaaataca gtgtatgttt actaatattc atctgtattt ggactttcat cttgtcatta
     4501 ttataaaaaa acgtatttca aaatataaat atacagaggg tgagatgttt tctaacatct
     4561 catctttttt taatggatca ttaatgaaag aagtttgaca ttataataat ggtagcgctt
     4621 tatgttaaaa tgaatagtga gtaatcagca atcaaattaa attggttgat agctgttaag
     4681 gtttgtggtt ttgtctttgt gctatcgcac ataaagtata taattaaagt agtttcgtta
     4741 ttataaaata ttaatataca tagtagatag taatagagca tcaccatggg aacctattga
     4801 gacacttatt gatttaaagt ggtattaata tgtcgtattt ctcgaacgtt ccattattca
     4861 ttttaaaaag ggggactgta tttgttatga caacacaaca tagcaaaaca gatgtcatct
     4921 taattggtgg cggtattatg agtgcaacat taggaacatt acttaaagaa ttatcacctg
     4981 agaaaaatat taaagtgttt gaaaaattag cacaacctgg cgaagagagt tcaaatgtat
     5041 ggaataatgc cggtacaggg cattcagcac tttgcgagtt gaactataca aaagaaggta
     5101 aggatggcac agttgattgt agtaaagcaa ttaagataaa tgagcagtac caaatttcaa
     5161 aacagttttg ggcatattta gttaaaacag gacaattaga taacccagat cgctttattc
     5221 aagcggtgcc acacatgagt tttgtcattg gcgaagataa tgtagctttt ataaaaagtc
     5281 gtgttgcaac gttaaagaaa agtgttttat tcgaaaaaat gaaattatcg caagatgaag
     5341 aagaaatgaa atcttgggta ccgttaatga ttgaaggtcg taagtctgat gaaccaattg
     5401 ctttaactta tgatgaaact ggtacagatg ttaactttgg tgcgttaact gcaaagttat
     5461 ttgaaaattt agagcaacgt ggtgtgggaa ttcaatataa gcagaatgta ttagacatca
     5521 agaaacagaa atctggggca tggctagtta aagttaaaga tttagaaact aatgaaacga
     5581 caacatatga atctgatttt gtatttattg gtgctggcgg tgcgagttta ccattactcc
     5641 aaaagactgg gattaaacaa tcaaaacata ttggtggttt cccggtaagt ggattattcc
     5701 tgcgctgtac aaatcaagaa gtgattgatc gtcatcatgc taaagtgtac ggaaaagcag
     5761 cagtgggtgc gccaccaatg tcagtgccgc acttagatac acgttttgta gacggcaagc
     5821 gttcattgtt atttggtcca tttgcaggtt tctcacctaa atttttaaaa acaggttcac
     5881 atatggattt aattaaatcg gttaaaccaa ataatatcgt gacgatgtta tctgcaggta
     5941 tcaaagaaat gagtcttacg aagtatttag tgtcacaatt gatgttatct aatgatgagc
     6001 gtatggatga tttaagagtc tttttcccaa atgctaaaaa tgaagattgg gaagtgatta
     6061 cagcagggca acgtgtccaa gtaatcaagg atactgagga ttctaaaggt aacttacaat
     6121 ttggtactga agtcattacg tcagatgatg gcacattagc tgcattactt ggtgcatcac
     6181 ctggtgcgtc aacagctgta gatattatgt ttgatgtttt acagagatgc tatcgtgatg
     6241 aattcaaagg atgggaacca aagattaaag aaatggtgcc gtcatttggt tatcgcttaa
     6301 cagatcatga ggatttatat cacaaaatta atgaagaagt aactaagtat ttacaagtta
     6361 aataataaat gaaacggtaa tgtctttttt aatgtgatag acattaccgt tttttagttg
     6421 ttaatgaaaa tcattttaat tgtttcagtt gcttgttaat agtgtttacg tagttcttat
     6481 ttttaaagaa ttgaattatc caaattaata cataaaccac aatgaagata attgtgaata
     6541 tgattagata atgcactgtt agtggaaacc aaccggcaag cattgctaaa ggcaagaatc
     6601 cgacatacgt tgttatgaaa tgcattatag ttgctttagt aatgctccaa tctgtgtatt
     6661 taaagataaa atctccaagg aaaaagacga cgcctatgag taaccataaa atgatagaaa
     6721 tcaacattac ggtagtttct gtgaaatgcg tataatacaa tatgccaata gttgattgtg
     6781 ggttcagtgg ataatatttg ccgtctgcaa ataacatact aaagaacagt gaaagggaca
     6841 aaccaatgat taagctaata aataatgagt ttttcaaatt tttcatattg ataagcgctc
     6901 ctttatagat tttaaataac gtctagaaga ataggtgtag tgtgcatctt taagatacat
     6961 acgtataagt ccatttggct ctaataataa tttttcaatg taatacttgt tgacgatttc
     7021 tgatttggaa atgcgaatga aatgttgtgg taactgtttt tctagttcat aaagtcgtaa
     7081 ttttagtttg aatttttgat tgcttgtaat ggctgttaca tttttattaa taacttggaa
     7141 tgttataaca tctgatacat taatgatatg gatttctttg tctatgtatc cgactaatgt
     7201 atgtgatttg tctaaatcat tgactgcatt aataatactt tgaacgtgat cattcatttt
     7261 aggtgcatgt atatcaatat aagattccgt ctcttttgca ttgataaata aattgagttt
     7321 catcataggt taatgcctcc ttcaaaatta ttaaaccata aatgaccatc gatatattta
     7381 aattttgttg aatggtagaa attaaatgtt aagtggctag aaagcgctaa tcaatataaa
     7441 agatacctcc tgaaataaaa acagaaatgt tttttcagga ggtagagatt aaagtgaatt
     7501 atttggcagt gtaatagtaa aggtggttac atactcgtta ctttgtgtga attggattgt
     7561 accatgatgc aattcaatga tggattttgt aattgcaaga cctaaaccat tgctattatc
     7621 atgtttgctc actttataaa aacgttcaaa taaacgtgct tcagcttgtg gactaattgg
     7681 tgaaccatca ttacttattg taaaaatgat gttgttgtga ctatgttgca aagcgatgtc
     7741 aatggcacca ccaacatctg tatacttaat agcatttatt aataaattac tcaatgcttg
     7801 atgtaacaaa cgttgatttc ctaggaaatt gattgattct agatcagcta aaataattaa
     7861 cgacttttca tcagcagcaa attgttcatg tcgaatgata tctttaatga gttgatcgac
     7921 ttgaatctta tcatcgaata ataaatgttg gtgattatct aattcggata agagcaacaa
     7981 ttcagttgtt aatccgctca gctgcgttgt aatggtataa atatcattta tatattgttg
     8041 tcttaatgta ttatcagaag tttgttgtaa ctcgcttaat aaatgatgaa tatgtgttaa
     8101 tggtgttttg atttcatgag atacattttg tacaaaatgc tgcctcattt gatcaacttg
     8161 tcccaatgac tctctcatct tattaaagtg atactgtaat gttccaattt catctttgcg
     8221 tgtttgtttg ataggtgttt caaaatcacc atcaattaag cgttcggtcg ctagctttaa
     8281 cttttttact ggacgaatga ttgaataggt tgatgcgata actaatgaaa tcgagataaa
     8341 taataacaac attaacaaga cggctaagaa tgttctaaac tcgctaaatg tttctccaat
     8401 atctggacgc atgaatactg ctattgaacc gtcttttgtc ttaaagttaa taccaacagt
     8461 attatctgtt acattgtcga aaaatccagt tacgaataat gcgaatggtt tatctttaat
     8521 accatggtaa tctttattat ttaatacgtt gttaatagca ttttgtgata aagtatcttc
     8581 tctaaatggc tccccataaa atgtcttatg acctttttga tcaacggtca ttatttggta
     8641 attcatttgc cctaaatgtt tgaaatattg ttgaatgtgc gttggtttag ctgattgttc
     8701 atattgtctt gcttctttaa gtgtcttcat gattttcgcg tcattagatg cttttaaatt
     8761 ataatgatag taaacatttg ttaatacaaa acttattaat gcactaaata aaataaccgt
     8821 aatggaataa atcgcaattc tagcatagag tgttttaaac atgattctcc accttatagc
     8881 cttgtcctct tactgtttca attgtaagtg tggcatttaa tttttttaat ctttggcgta
     8941 gtcgcttaat atgaacgtca actgttcgtt catctccttc ataatcatag ccccaaattt
     9001 tttctattat ctgttcacgt gtaaagattt gcttaggacg tgctgcaagc ataaataata
     9061 attgaaattc cttgttcggt aacgtcatcg ttttattaga tacttggagt tccaaatagg
     9121 attggtttag cgttaagttg ccaatagtca tttctgaatt tgaattgata ttatatcgac
     9181 gtaataccgc acgaattcta aaaataagtt ccttaacctc aaagggtttg gttacataat
     9241 cgtcagtacc gcttataaac gcacgctctt tgtcactaag tgcatcccgc gctgttaaca
     9301 taataactgg tatatcataa tcatttttta atgtattaca taattgaaag ccgtccatac
     9361 catccatcat aatatctacc actgcaatat cgacacgctg tttttctaaa agttttaatg
     9421 cagcttcacc acttggttgt gtgtatgcat caatgtgctc tgtttgtaaa tggctagcta
     9481 tataatttag gattcgagga tcgtcatcga caacaagaca ttgcaccata gctataaact
     9541 cccttatctt tttcatttat tatacatgta aaatattttt gcgtaaaaaa acaattgttc
     9601 atattgagtt catatttcaa ccttatactg acgctaaaga agaaataggg agaagtgaat
     9661 cgatatgaaa ttagcgataa aagagattat gttttacaaa tttcgttata ttttaatcac
     9721 attaattatt cttttattaa gtattatggt gttatttatt agtggtttag ctcaagggct
     9781 tggtagggag aatatttcgt tatttgaaca ctttgataat gatgaatatg ttgttcaaaa
     9841 aatgaaagag ccgcaaattg agaaatcgca acttagtgat acgcaacaga atcaaattaa
     9901 aaaggtaatt catcaagaac catataaaat gaatattcaa acattgaaat tatctaataa
     9961 agaacaagat gtgattacaa tgaatgacgt taaacaacaa cgcatacaac ttaaaaaagg
    10021 tgattatcct aaaaatgctc atgaggtcgc tattaatgat aagttagctg cagacaacat
    10081 tagagtcggg gatagattac attttaaaaa taattcaact agttatagag tttctggtat
    10141 tttaaacgac acaatgtatg cgcatagttc cattgtgcta ttgaacgata acggatttaa
    10201 tgcattgaat aaggttaata cggcatttta tccagtgaaa aatttaacac aacaacaacg
    10261 tgatgagctt aataaaataa atgacgttca agtggtgagt gaaaaagatt taacaggtaa
    10321 tattgcgagt tatcaagcag agcaagcacc gttaaatatg atgattgtta gtttgtttgt
    10381 tattacagca atcgttctaa gtgcattttt ctatgttatg acgattcaaa aaatatcaca
    10441 aattggcatt ttgaaagcaa ttggtattaa gacaagacat ttattgagtg cgttagtttt
    10501 acaaatttta acactaacaa taattggggt aggtattgct gtgatcatca tagtaggact
    10561 atcatttatg atgccggtaa cgatgccttt ttacttaaca acgcaaaata ttttattaat
    10621 ggtggggata tttatattag tagcgatttt aggtgcctca ctatcattta tcaaattatt
    10681 taaagtggat cctatcgaag caattggagg tgcagaataa tggcattagt cgttgaagat
    10741 atcgtcaaaa atttcggaga aggtttgtct gaaacaaaag ttttaaaagg tattaatttt
    10801 gaagtggaac aaggggaatt tgtcatttta aatggtgcct ctggttctgg gaaaacaaca
    10861 ttgctaacga tattaggcgg attgttaagt caaacgagtg gtacagtgct ttacaatgat
    10921 gcgccattgt ttgataaaca gcatcgtcct agtgatttac gattggaaga tattggtttt
    10981 atttttcaat cttcacattt agttccttat ttaaaagtga tagagcaatt gacactcgta
    11041 ggtcaagaag cgggaatgac caaacaacaa agttcaacaa gagcaataca acttttgaaa
    11101 aatattggtt tagaagatcg cttgaatgta tatccgcatc agttatctgg cggtgaaaag
    11161 caacgtgttg cgattatgag agcatttatg aataatccga aaatcatttt agcagatgag
    11221 cccacagcaa gtttagatgc cgatagagca acaaaagttg ttgagatgat acgtcaacaa
    11281 attaaagaac aacaaatgat tggtattatg attacacacg atcgaagatt atttgaatat
    11341 gcagatcgag tgattgaatt agaagatggc aaaataactg attagtggct tgtaaagacg
    11401 ctaaatgtta atgatttaag acatagtagt ataaaagtta gataacagaa tacgatttgg
    11461 gtttacaaaa aagcacgtca ctattatata aaatgatgac gtgctttagt ttatttattt
    11521 tcaatataac aattgtctaa tcaaggatta acgaatattt aaagatagtt tgacgcaata
    11581 ttagaaacaa cctataataa tagtttgttt gtggattaac tattataaat aaaagcggcg
    11641 taaagacata taaaccaact acttgaacaa tataacgtta ataacaatct atactgatac
    11701 attacgccta gataatcttt aatgagcaca tgtaagaaaa agtgatatgg tgtatgactt
    11761 ccgacaccat cgatagataa acctaatttt tgggctagtc gtaaggcgcg taatacatga
    11821 aactgacttg ttacacaaac aattttaact gcttcatgat acaaattgtt gatgatttgt
    11881 ttagaatata aaaagtttgt gtatgtattt atagagtgag attccattag tatatctgtt
    11941 ttatcaacac catgtgcaat caaataacgt tgcatagcta aagcttcaga aattggttcg
    12001 tctggtcctt gtccgccaga tacaatgatc tttgttgctg atgcttgttg ttgatagata
    12061 tcaagtgcac ggtctaaacg cgctgcaagc attggtgtga caaattcggt aaaaatacca
    12121 gcacctaaca caattatgat atcaacttct ttgttgtatg atctatgtct atatgatact
    12181 gtccaaacga gataacaaat aaaggttagt aacagggaaa gacataatat agctaaccac
    12241 atagacaaac ctttcacaat aggtgactga atcgtactta taaatagaag tgctgatgtg
    12301 tagagtacaa atttatatga aaaagataat aattttttaa taaataagcg actagaagta
    12361 tgagaaaata aatatctatg tttgaatagc atgataatac tgattattat aaatgttaca
    12421 aacatagacc aagggaaagt ataggtcatg atgctataga tgagtgacaa aaatatcgat
    12481 atgacaacta agatgtagca tgttaaattt aacgtcagag tatagttgaa aattaacgga
    12541 caaataacga taagtataaa tattaataat aaattcaa
//
LOCUS       DBNENW010000071        12576 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNENW010000071 DBNENW010000000
VERSION     DBNENW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.071"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..537)
                     /gene="radC"
                     /locus_tag="AC1LW8_001585"
     CDS             complement(<1..537)
                     /gene="radC"
                     /locus_tag="AC1LW8_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIP4773416.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGD"
     gene            complement(534..1241)
                     /locus_tag="AC1LW8_001586"
     CDS             complement(534..1241)
                     /locus_tag="AC1LW8_001586"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIP4773417.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(1511..2782)
                     /locus_tag="AC1LW8_001587"
     CDS             complement(1511..2782)
                     /locus_tag="AC1LW8_001587"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIP4773418.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(2795..5425)
                     /locus_tag="AC1LW8_001588"
     CDS             complement(2795..5425)
                     /locus_tag="AC1LW8_001588"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIP4773419.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(5469..5770)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5828..6388
                     /locus_tag="AC1LW8_001589"
     CDS             5828..6388
                     /locus_tag="AC1LW8_001589"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIP4773420.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     gene            complement(6611..7684)
                     /locus_tag="AC1LW8_001590"
     CDS             complement(6611..7684)
                     /locus_tag="AC1LW8_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HIP4773421.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7718..7819)
                     /locus_tag="AC1LW8_001591"
     CDS             complement(7718..7819)
                     /locus_tag="AC1LW8_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773422.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(7880..9166)
                     /gene="hemL"
                     /locus_tag="AC1LW8_001592"
     CDS             complement(7880..9166)
                     /gene="hemL"
                     /locus_tag="AC1LW8_001592"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIP4773423.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRNITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(9214..10188)
                     /gene="hemB"
                     /locus_tag="AC1LW8_001593"
     CDS             complement(9214..10188)
                     /gene="hemB"
                     /locus_tag="AC1LW8_001593"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIP4773424.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(10191..10859)
                     /locus_tag="AC1LW8_001594"
     CDS             complement(10191..10859)
                     /locus_tag="AC1LW8_001594"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIP4773425.1"
                     /translation="MKPVVVMTQTNDMQSDLASIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(10881..11807)
                     /gene="hemC"
                     /locus_tag="AC1LW8_001595"
     CDS             complement(10881..11807)
                     /gene="hemC"
                     /locus_tag="AC1LW8_001595"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIP4773426.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(11849..>12576)
                     /gene="ccsA"
                     /locus_tag="AC1LW8_001596"
     CDS             complement(11849..>12576)
                     /gene="ccsA"
                     /locus_tag="AC1LW8_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIP4773427.1"
                     /translation="KSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSISDVFY
                     TLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAVVDELL
                     LVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIVFYSTL
                     SGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWLNSRYL
                     IYYNIILFCLNMINLFFATHFVN"
BASE COUNT         4147 a         2387 c         1732 g         4310 t
ORIGIN      
        1 atcaccggat ggatgattat gaactgcgat gattgcattg gcattttctc tcaccgcaat
       61 actaaaaatt tcacgtggat gtacaatcga actatttaat gtacctttaa aaacacaggt
      121 ttctttaatc actacatttt ttgaatttaa caataaaatg acaaaatgtt cttgtgttaa
      181 atctttcatt gttggaatca tataatcagc aacatcactt ggttgcgtta tttttatacg
      241 attattttca gctcttctcc ccatcctttc ccctaactca aatgctgctt ttaaagtaat
      301 tgctttttgt aatccaatcc ctttaacttt tatcaaatcg ttaattgaag attttttcaa
      361 ttcattcaga ttcgaagcag atttaagcag ttcattacta atgtctatgc tcgagaatcc
      421 ttttcttccg gtgttaatta atatagctaa taattctgta ttcgaaagac tttttgcacc
      481 atggcttaac aaacgttctc ttggcatttc tgaagttacc atttctttaa ttttcaaaaa
      541 tatacgcctc ctaaaaattg atggatatca ttataaaaaa gtgaattgat aaaaaaggaa
      601 ataaatataa atggaacaag gggtaatagt ttaatcggct taaatatcat ggtaattaaa
      661 gcaactaaac cagcaatgac aaatgtaaat aaaatgacat aaatagtgaa ttggagaggg
      721 aaaaacaaag aaagtgcaga tattagtaaa acgtcaccat aaccaatata tgcccgaaat
      781 aaaaagtaga atatatgcgt ggtcatacta ataatgataa aagctactgg ataaatcata
      841 cttaacgaga gagaaacgat acaataaatt ataattaagc gacaatctaa cattaaagaa
      901 gtgatatcgg tcatagtaaa aataagcaga aaaacatatg tagttataaa tagcgtagca
      961 tttacgtatg tgaaatcata cttaataaag acgataggta ttaaagcaaa ggtttcccct
     1021 aagaaatgtg ttagggaaat acgctttcga cagtttcgac atcgcccttt taataataaa
     1081 aaactaataa tcggcattaa ttcataccat ttgagtgacg aattacaata atcacatttc
     1141 gatcttctat gtaaataatc aaatgacgtt tcttctatag atataaattg atatagaaaa
     1201 ctaaaaatac aactgcaact ataagataac aatactacca aatgacaacc tccttatgta
     1261 aattatagtt agttattacc aaaatgtaaa tatacactat ttttcaagaa ttgaaccgct
     1321 ttttcattta aatttttcaa tattgctaag cataattgat ggatacttta acaacccatt
     1381 actgctcggc aaaattaata atggcaagaa attgaacctt ataaacacat acgatttaga
     1441 gcataaaaaa taaccatgaa gctctaccta ttgattaaat agattcttca tggctatttt
     1501 agttttagtt ttataatgct tcaaagtcta attttgattt aacttcactt atgaaataca
     1561 gactaccggt aattactaat gtatcacctt gataattttt tataaattca acgtagtcat
     1621 ctactaattg tatttcatca ttttcaatac tacctacaat ttcttctttg cgtaacgctt
     1681 tcggaaaatc aaattcagtt gcataaaacg tatgcgcaat taaacttaaa tgtttgacca
     1741 tctcgttaat cggttttccg tttattgctg agaacaaaat atctactttt tctttatcat
     1801 ggtactgttt aattgtatca attagagcat ctatactctc tgaattatgt gcgccatcca
     1861 aaatgattaa aggtttgtca tgcacctgct caatacgtcc agtccaacga actgattcaa
     1921 taccgtctat catcttattg aaatctaatt caattaatcc ttgttcattt aattcaataa
     1981 gagctgttat ggctaatgca gcattttgtt tctgatgttc acctaacatg cttaaaatga
     2041 ttgtttctaa ttcataatct ttataacggt aagtaaattc atcattttgc gatacaacaa
     2101 caatttctct atctaattca attggctttg catgttgttc aattgcgcgt tcacgaacat
     2161 attttaatgc atcttcattt ttaacagcat atatcactgg aacgttaggc tttataatcg
     2221 cgcctttatc cctagcaata tctagataag taccacctaa aatatctgta tggtctagac
     2281 cgatactagt taagattgat aaaaccggtg taaagacatt tgtcgaatcg ttctttatac
     2341 ccaatccagc ctcaacaatg acaaaatcaa caggatgtat ttcaccaaaa tataaaaaca
     2401 tcatcgctgt gattatttcg aattcagttg caacacctaa atctgtttca cgttccatca
     2461 tttcacttac tggtttaata cgtgatacta attctacaat agcgtcattt gatattggca
     2521 caccatttag actaattcgt tcattaaatg tttcaataaa cggcgacgta aatgtaccta
     2581 cttcataacc attttcaact aaagctgttc taaggtaagc aactgtagag cctttaccat
     2641 ttgtgccacc tacatgaata cccttaatgt tattttgagg attattaaat tgtgctagca
     2701 tccattccat acgtttaaca cctggtttga tgccaaattt agttctttcg tgtatccaat
     2761 acaagctctc taggtaattc attgttacta actcctatgc ttttaattgt tcaattcttg
     2821 ccttcacacc atcatatttt tcttgataat cttgtttttt acgtttttct tcatttataa
     2881 ccttttcagg tgctttactt acaaagtttt cattagagag ctttttatct actctatcta
     2941 attcgctttg aagtttagct aattcttttt ccaaacggct gatttcctta tccatatcaa
     3001 ttagcccttc taatggtaat accactttac ctgcaattac aactgatgtc attgctttct
     3061 caggaatttc cacgtcagtg ctaatattta aggtactagg attacagaat ttgattaaat
     3121 aatctttgtt ttgtgataaa gttgtttcaa tttctttatc tttagcttga attaaaatag
     3181 gtatttcttt agacaatggc gtatttactt ctacacgtga ttgtcttaca gatttaatga
     3241 tttcaacaag ttgttgcatt gtttgtttac tttcttcaaa aatcaatgat tcacgcactt
     3301 ctggccatga agctttaaca attgtgtcac cttcatgtgg taaactttgc catattttct
     3361 ctgttacaaa tggcatgaat ggatgtagca ttctcataat attgtctaaa gtataactca
     3421 atactgaacg tgtaacttgt ttttgttctt catcattact attcattgga attttactca
     3481 tttcaatgta ccaatcacag aaatcatccc aaatgaaatt atataatgca cgtccaactt
     3541 cgccgaattc atatttgtca cttaaatcag taactgttgc aatcgtttca tttaaacgtg
     3601 ttagaatcca tttatctgct aatgataagt taccacttaa atcgatatct tcaactttaa
     3661 agtcttcacc gatattcatt aaactgaaac gtgccccatt ccagatttta ttgataaagt
     3721 tccacactga ctcaactttt tcagttgagt atcttaaatc atgtcctgga gatgaacctg
     3781 ttgctaagaa gtaacgcaag ctatcagcac cgtattcgtc aataacatcc attggatcca
     3841 caccattacc taatgattta ctcatcttac gcccgtcttc agcacgaact aaaccgtgta
     3901 ataatacatc attaaatgga cgacgatctg taaattctaa gccttggaat atcatgcgtg
     3961 ctacccagaa aaagataata tcgtaacctg taactaaggc atttgttggg tagtatcgtt
     4021 taaagtcttc actttctaaa tcaggccaac ctaacgtaga gaaaggccat aaagcacttg
     4081 agaaccacgt atctaataca tcttcatctt gttgccaatt ttcaatatca gttggcgctt
     4141 cttctccaac atatatttcg cctgtttctt tatgatacca agccggaatt tgatgacccc
     4201 accataattg tcttgaaatc gtccaatctc taatattttc catccattgg ttaaatgtat
     4261 gttcgaaacg ttgcggataa aaatcaatac gatcatctgt tttttggtta tctaatgaac
     4321 gtttcgctaa gtcttccatg cgcacaaacc attgtgttga taaatatggt tcaacaacag
     4381 cgccagatcg ttctgaatga cctacagaat gaacatgatc ttcaatcttg ataactaaat
     4441 cttgttcttt taaatcttta actagctgtt tacgacaatc aaaacggtcc ataccttcat
     4501 atttacccgc tttgtcgttc attttaccat tttcatccat aacgataata ttttctaatt
     4561 gatgtctttg accaatttca aaatcattag ggtcatgtgc tggtgtcact ttcatagcac
     4621 cagaaccgaa gtctatatca acatactcat ctgctaaaat aggcagttcg cgtcctacga
     4681 ttggtaatat aacagtttta ccgattacat ctttgtatcg ttcgtcatta gggttaacaa
     4741 caatcgctgt atcacctaac atcgtttctg gtcttgttgt tgcaatttca ataaaacctt
     4801 caccatcagc gtaaggatat ttaaaatgat aaaacgcacc ttgaacatct tcatgtatta
     4861 cttcaatatc agataaagct gtacgtgctt taggatccca atttataata cgttcgccac
     4921 gataaataat tcctttattg tataaatcaa caaaaacttt tttaactgct ttacttaaac
     4981 cttcatctaa agtaaaacgt tctctactat aatctaaacc tagacctaat ttagcccatt
     5041 gcgcacgaat aaatgacgca tactcttctt tccaatccca tgcctgttct aaaaactttt
     5101 cacgaccaag atcatatcta gttattcctt gttcatttaa tttagcttct acctttgcct
     5161 gtgtcgcaat accagcatga tccatacctg gtaagtataa cgtatcgtat ccttgcatac
     5221 gtttcatacg tgtaatgata tcttgtaaag tcgtatccca tgcatgtcct aaatgtaatt
     5281 taccagttac atttggtggc gggataacaa ttgtatatgt ttcttttgat ttatcttctg
     5341 acggtttaaa ataaccattc tttacccatt cttcataacg tcccgcttca acttcacgag
     5401 gatcatattt tggtttcatt tccatttgct atacctccta aaaaaataaa aatatccatc
     5461 ctatatacaa ataggacgga tattccgtgg taccacctat attcaagaag gatgtttaat
     5521 atcaaattca ctcttttaac ataattggaa taatcatacc aatactatca tcgtgaaatt
     5581 tgaaatgctt catctcttca agcactctag attatgatta acgctcaaac acgtcttagc
     5641 ctactattaa tcacgttcag ctaagatact ctgtgggcta ccttcagtaa aaatcattta
     5701 catactcaca ccaaatcata tgctctcttt aaaataattt gaacttactc ttcccaaatc
     5761 ctatattaaa ctcttaactt atagtataat gattgacaaa ataagtcaat gtataggtgg
     5821 gaataaaatg aatgaatgcg catttggtac taaagatcca gtctacttaa actatcatga
     5881 tcatgtatgg ggacaaccgc tctatgatag caaggcattg tttaaacttt tagcattaga
     5941 atcacaacat gctgggctat cttggttaac tattttaaaa aagaaagaag cctatgaaga
     6001 agcattttat gatttcgaac cagaaaaggt agcacaaatg accgctcaag atatcgaccg
     6061 cttaatgact tttccaaata tcgttcatca tcgtaaaaaa ttagaagcaa ttgttaatca
     6121 agctcaaggg tatttaaaaa ttgaacaagc atatggtagt tttagtaaat ttttatggtc
     6181 ttatgtaaat ggtaagccta aagatttgca gtatgaacat gcttctgatc gtatcacagt
     6241 tgatgatact gcaacacaac tatctaaaga tttaaaacaa tacgggttta aatttttagg
     6301 tccagtaaca gtattttcgt ttttagaagc agctggttta tatgatgcac atttaaaaga
     6361 ttgtccatca aagcctaaac acaattaatt tgctatagca tcataaagta aacgagacat
     6421 catctatttt taactaatag actgtctcgt tttttgatgt gaaaatttat tcaaatcata
     6481 agttaaagtt ccaagttaat gcactgctaa tattagatat cttaatctaa aaatagatgt
     6541 acaacctttg cttaaacctt aataatgaat tctatcctaa gcataaatca acttaaatgg
     6601 atgatagttt ctaatcttca cgcttattcg aacgatattt taaaaagtaa ttaattcctg
     6661 gtgcaataat aaataggatg aaaaagattc ggaaaatatg ataactcgta atcatagcaa
     6721 catcggcacc agtagctaat gcaactaaaa ctatctgatt aacccctcct ggtgctgcac
     6781 caagaaacaa ttcattaata ggattattat caaagaaatg tatgacataa accatgatta
     6841 gcgcaccaat tatcaacata atattttgaa ttgtaattgc gattgctagt ctacctttta
     6901 aatctgacaa taaatgcgca atttgaactc caattctaat catatatatt agttgtgcca
     6961 tgttcaacaa ccaatgatct agtgtaaatg ttaaacctgt agaaaaattc caaacaatta
     7021 atacaatgag tggtgctaat aattgaaatg ttggaaactt tattttagac ataattagat
     7081 aaactataaa gatagctatc gctaaaataa ctatttgccc tatgtttaat acttgtgata
     7141 aaggcaagac ttttgttaac tttccattcg catgcatgtt accatcatga aaaaaatatg
     7201 aaatgaacgg tactaaaaca acaacaaata taattcgtga tgtttgcgtt aagctaacaa
     7261 ctaacaaatt agcacgtttg tcttgttcag ccatgaccag catttgtgtt agtgctcctg
     7321 gtataacact taaaatagct gtttctgtat taatacgtgc aattttttta aaaataaatg
     7381 ccattactat tgcaattaat aatatcgaaa tagatacaac aataatcgaa agccaattgt
     7441 ctttaatatc cataacgaca tttttcgtaa acgttgatcc gatttgcaca cctaatagta
     7501 caatacctaa ttcactaagt aagaatggcc atttaatatc aagtttgaaa acttttacac
     7561 aaatgattga tgcgataata ggaccaaaca taaatgggag taatacgtgc gatgaataca
     7621 gtagaatact aataaaaaat gataaaacga acacaatgaa attatttcta tatatcattg
     7681 tcatgttttc cacttctttc aataaaaaat aaaatgacta aattgctgct tgagcttcac
     7741 gtttgttaag ataacaatat ccgctagcag ttttgactac aaagcatata tggactttca
     7801 ctatcaagtc gccgcccatg cctgatatac atttaaaaag agcctgaaca aagttcaggc
     7861 tctcaatttg tccgtatatt tattttacaa tacgacttaa agccgtatca aatgcttgaa
     7921 tcgttttttc aatatcttct ttcgtgtgtg ccgtagatag gaatgtacct tcaaattgag
     7981 atggtggtaa aaacacacct tcttttgcca tttctcgata catttctgca aataatttca
     8041 aatcactttt attcgcttgt tcaaaattag ttacaggtcc ttcatttaag aaataaccaa
     8101 tcattgaacc tgctctattt acagttattg gtacattgtg tttagcaaat acacgcttta
     8161 aaccgtcttc aagtatatcg cctaacatat taaaatactc atatgtctct ggcgttaatt
     8221 ggcttaacgt ttcataacca cttgtcattg caagaggatt tcctgataac gtacccgctt
     8281 gataaatatt tcctaatggt gctatatgat ccatgatttc ttttttacca ccaaaagcac
     8341 ctacaggtag tcctccaccg ataacttttc ctaagcaagt taaatctggt gtcacaccaa
     8401 agtaaccttg tgcacaatga taaccgactc tgaaaccagt cattacttca tcgaaaatta
     8461 gcaatgcgcc gtattcagtc gtaatatttc ttaatccctg taaaaaacct tcaatcggcg
     8521 gtacgacacc catattacca gcaacaggtt ctacgattac accagcaata tcgtctccaa
     8581 atttttcgaa agcgatttta agtgcatcta aatcattgta tggaactgta attgtatttt
     8641 tagcaatacc ttcaggcaca ccaggagaat ccggcaatcc taatgttgcc accccagaac
     8701 cagctttgat taataacgaa tcactatgac catgatagca accttcaaat ttcacaattt
     8761 tatttcttcc agtataacca cgtgctaatc ttaaagtatc caatgtagct tctgtaccag
     8821 atgacaccat acgcactttt tctattgaag gtactcggtc aataacgagc tgcgccaatt
     8881 tattttcaag taatgttgat gcaccaaaac ttgtaccttt atcaattgct tcatgtaaat
     8941 gactaataac ttgagggtct ctatgtccta aaataagtgg cccccaactt agtacatagt
     9001 cgatatactc gttaccatcg atatcataaa tttttgaacc tttaccgtga tccataaaaa
     9061 ttgctggtgt atctactgat ttaaatgcgc gtactggact atttacacca ccaggcatta
     9121 aagtttcagc aaccttcatt gcttcttctg atttcgtata tctcataaag taaattcctc
     9181 ctatagttta tggaaaatca tacatataaa accttattta tctaaatagc gacaaatgtc
     9241 ctttgcaaaa tacgtaataa tcatatcagc acctgcacgt ttcattgaaa ccatttgttc
     9301 cataacgaca cgttcttcat ctatccaacc attttgtgcc gctgctttag tcatactata
     9361 ttctccactc acattatatg caacaactgg aacattcgta tgatttttaa catctcgaac
     9421 tatgtctaaa taacttagag caggtttaac aatcatcatg tcgcaccctt ctttaagatc
     9481 actttctaat tcacgaagtg cttccaaacg gttagcaggg tccatctgat acgtttttct
     9541 atccccaaat gatggcgctg aatctgctgc atctctaaaa ggtccaaaga aacttgatgc
     9601 atacttgaca ccataactca ttataggaat attgtaatag ccggcttcat ctaatccacg
     9661 acgaatttca gcaacaaaac catccatcat attacttggc gcaataatat cagcaccagc
     9721 ttccacttga gaaattgctg ttttaacaag cagtggcaat gatttatcat tgtcaacgtc
     9781 atgtgtatgg tcatcaatca cgccacaatg accatgatca gtatattcac ataaacaagt
     9841 gtctgcaaca attaataagt catcatacat ttttttagca atacgtgttg cctgttgaat
     9901 aacaccatcg tgaatgtatg caccagtacc tatatcatct tttgagtttg gaacaccgaa
     9961 aaacataatg gcacgtatgc ctaagtcata agcttctttt aattcacttt caagtaaatt
    10021 caaactgatt tggtatacac ctggcaatga cttaatttct tttttcacat cgtctttttc
    10081 aactacaaaa attggatata ttaaatcttc ttttcttaca tgattctctc taaccatatc
    10141 tctcattgtc gctgatgatc tcaatcttct atgtctatca aatttcattt ttagccccta
    10201 ctttctaaaa tcttttcaat tagtgattcg agtgtttgaa tttctgcaat tgttactggt
    10261 tgttgatatg atttaatggt ccgtgctgtt tgttctccaa tagcaaaata cgacttgaat
    10321 tttggtacaa atccttcatt aaaataataa cgtactgccg acgaacttga aaatgttaat
    10381 gcatcgattt gttgatgttc tatcatttct ttaacatctt gtatattttg tttgttaggc
    10441 actgaagtat ataaatctat tttaacaact tcattatctt tagataacgc tgctaataac
    10501 aatggtctcg ccaattcact cgaaggcaaa agtatttttt ggttagtttg attaaatgat
    10561 tttaaaaatc cttcttgaga aaagtcgttt ggcataaaat caactcgaat gccaagtgat
    10621 tcacaatatt gcgctgtctt acttcctatc acagcaatgt tatcaacatt aattcctttt
    10681 agatatttat aaaagaattt cacagcattt ttagatgaaa aaataagcca gtcatagcgt
    10741 tgatttaaca aatgaatatc aaaatttagt ggctttatat caataaaggg tttgtgaata
    10801 attgatgcta aatcactttg catgtcattt gtttgtgtca taactacaac tggcttcata
    10861 tttaaacgtc actccattat ttaatgttgt tcatttaagc gttttataat ttcataagca
    10921 ccttgctctt ttaatttgtt actcactgtt ttgcctaact caaccggatc tgttccgttc
    10981 attgtatatt caaatcgttc tttaccatct ggggtcataa ttaaacctgt aaattcgatt
    11041 tctttttgat ctgagattgt agcatatcct gcgattggca cctgacaact accatccatt
    11101 tctgctaaaa acgttcgttc agcagtcaca cattttgcaa cctcatcatt atgtactttg
    11161 cttaataatg ttaatagttc ttcatcgtca ctacgacatt ctatccctaa agcaccttgt
    11221 ccgattgcag gtaacaatgt atctctatca agataagatg ttacaatatc atctgaccag
    11281 cccattcttc ttaaaccagc tgcagctaaa ataatcgcat cataatcttc agtttgtaac
    11341 ttttctaatc gtgtatctat attacctcta atccatttaa tctctaaatt aggatactta
    11401 gataatattt gtgcaccacg acgtaatgaa ctagtaccaa taatactgcc ttctggcaat
    11461 tgggatagtg gtgtatgtgt tttagaaata tacgcatcaa aaggtaattc tctatcaggg
    11521 atacaaccta atgttaaacc ttccggaatt acacttggta catctttaag cgagtgtatt
    11581 gccatatcga tatttttttc aaaaagttca tgttgtattt ctttaacaaa taagcctttg
    11641 cctccgactt tagacaattg cttatctact atacgatcgc ctttcgtgac aatttcttta
    11701 atttcaattt ctagatttgg ctcgacagct tttaatttat caataaattg ctggctttgt
    11761 gttaaagcta atttacttct tctggagcca acgactaatt tacgcatgtt caattcctcc
    11821 taggaacgga ttgctctaga ttattttctc aattcacaaa atgtgttgca aaaaataaat
    11881 taatcatatt taagcaaaat aaaataatgt tatagtatat taaatacctt gaattcaacc
    11941 atttgttgat tctaagcaaa atataacttc catataatac tgtaataatt gaagagagta
    12001 ttaccttcgg gtcaatgaat atacgttcac caactgaaat tacaccccac tgtgtaccta
    12061 aaataatact aaatatgaga attatccacc cacttaacgt tgagtaaaac acaattgatt
    12121 caagtgtagc aacgctacct attctaaagt atttttgatc aaaacgtttt tccttcaaat
    12181 tacggtattg catgatatac agtaatgcat tgacaaaagc taaggcaaag aagacataac
    12241 ttaacacagc tagaccgata tggactaaca gtaactcgtc tacaacagca attttctgaa
    12301 ccttattagt ataatgtgtc ggttgaaatg tattcatccc taaaagtgtt agccctatta
    12361 aattcaagaa aaacacagag aaattcaaaa ctttgataag gttaagaatt aaagagattg
    12421 aaataatcag ccaacttaaa gtatagaata catctgaaat agaccctaat ggaatatgtc
    12481 tagtttgtat aataaaaata gataaagaga ttgtttgtaa aacccaaaca atccccaata
    12541 aatatatgcc taaacttcta atcttatgac tttttt
//
LOCUS       DBNENW010000072        12539 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNENW010000072 DBNENW010000000
VERSION     DBNENW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.072"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..227
                     /locus_tag="AC1LW8_001597"
     CDS             <1..227
                     /locus_tag="AC1LW8_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HIP4773428.1"
                     /translation="SAKYQLTNDDYNVKQLRERYDIPTNKAPKLLLKGSGNLKGSSVG
                     YKNIEFTFVEEKGKNIYFSDSLDYKKSGEV"
     gene            230..1606
                     /locus_tag="AC1LW8_001598"
     CDS             230..1606
                     /locus_tag="AC1LW8_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773429.1"
                     /translation="MAKAEYEIDPGKITSNSEETSAISNISYEIENANDNNLDNEKIR
                     GQITKLKKGGNFPKNLDYIDSYTDPSTGTTATAFLNKDTGKVTVGMAGTNFHGDQLKR
                     VALSSMSPLLFPPSKQDMKDVRGTMKDGVADLAIGVGMVNYKGKHFANTQQFIENLQK
                     KYEIDTVTGHSLGGRDAIFLGLRYNIKNVVAYNPAPLEVKSIRNKFGGQLFRNTTFPD
                     EKYLKELMDNYDGDITKVITQKDGLDYLVKRTDHLTCGDVLRINNGQGHAMENFLGEK
                     EQREIIEELMLVKGYRDANDKAFKALKKNTEKKLGKIDEIKSKLLQTNGGALSSSQQK
                     LLETLVAFSVAEGLSKMVDQELQQLKNMFNLMDEKFEANWKDAQEASDIVGKHLSYPE
                     KVSALDNGGVNESKLATEPHNEIKDKLNKITDLSKKYNSYLQQIEKSINEIVAKDQQL
                     AGQIGDLI"
     gene            1606..1932
                     /locus_tag="AC1LW8_001599"
     CDS             1606..1932
                     /locus_tag="AC1LW8_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773430.1"
                     /translation="MFEEYKKIDDLENAYEIELKRIEREIQNLSDLKYHLRRENEQSY
                     DAFLYLKNKMNYSEESNAKVRRLVEEFDYEADTYIRQKELKLEDYKEEIRREYIQQSE
                     KIMEAK"
     gene            1936..2247
                     /locus_tag="AC1LW8_001600"
     CDS             1936..2247
                     /locus_tag="AC1LW8_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773431.1"
                     /translation="MGIPDPLSNPMSDPLLGDSWMVGDLKKRQEKKLITTVIAPEHDE
                     KHRENKSKMKKSEKQLINQLINHGHTFKSDFKNVAKGDWVNKAMQDLDKLGNELKKIM
                     N"
     gene            2499..4736
                     /gene="aryK"
                     /locus_tag="AC1LW8_001601"
     CDS             2499..4736
                     /gene="aryK"
                     /locus_tag="AC1LW8_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AryK"
                     /protein_id="HIP4773432.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHNALIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYFIDLIHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4887..6065
                     /locus_tag="AC1LW8_001602"
     CDS             4887..6065
                     /locus_tag="AC1LW8_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIP4773433.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNIGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIETEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6067..7455
                     /gene="norC"
                     /locus_tag="AC1LW8_001603"
     CDS             6067..7455
                     /gene="norC"
                     /locus_tag="AC1LW8_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorC"
                     /protein_id="HIP4773434.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7942..9603)
                     /locus_tag="AC1LW8_001604"
     CDS             complement(7942..9603)
                     /locus_tag="AC1LW8_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIP4773435.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9927..11702)
                     /locus_tag="AC1LW8_001605"
     CDS             complement(9927..11702)
                     /locus_tag="AC1LW8_001605"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIP4773436.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYGVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPQKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            12053..12526
                     /locus_tag="AC1LW8_001606"
     CDS             12053..12526
                     /locus_tag="AC1LW8_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HIP4773437.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT         4302 a         1971 c         2278 g         3988 t
ORIGIN      
        1 attcagcgaa atatcaacta actaatgatg attataatgt aaaacaatta cgtgaaagat
       61 atgatatacc gacgaataaa gcgccaaagt tattgttaaa agggtcaggg aacttaaaag
      121 gttcatcagt tggatataaa aatatagaat ttacttttgt agaggaaaaa ggtaaaaata
      181 tatactttag tgatagctta gattataaaa aaagtggaga agtataatta tggctaaagc
      241 agaatatgaa attgatcctg gaaaaataac aagtaattcg gaagaaacta gcgcaatatc
      301 taatattagt tatgaaatag aaaatgcaaa tgataataat ttagataatg aaaaaattag
      361 agggcaaatt actaaattaa aaaagggtgg gaattttcct aaaaacttag actacataga
      421 tagttataca gaccccagta cggggaccac ggcaacggca tttttaaata aagatacagg
      481 taaagttact gttggaatgg ctggtacaaa ctttcacggc gatcaactta aaagagtagc
      541 acttagttcg atgtctccgt tattattccc accctctaag caagatatga aagatgtacg
      601 aggcactatg aaggatggtg tagcggattt agccattgga gtaggaatgg tcaattataa
      661 aggaaaacat tttgcaaata cacagcagtt tattgagaat ttacaaaaaa agtacgaaat
      721 tgataccgtt actggtcatt ctttaggagg gagagatgct atctttcttg ggcttcgtta
      781 taacatcaaa aatgttgtgg catacaatcc tgctccgtta gaagtaaaaa gcattcgtaa
      841 taaatttggt ggtcaattgt ttagaaatac gacatttcca gatgaaaaat atttaaaaga
      901 gttaatggat aattatgatg gagatattac taaggttata actcaaaagg atggattaga
      961 ctatttagtg aagcgtacag atcatttaac ttgtggtgat gtgttacgta taaacaatgg
     1021 tcagggccat gcgatggaga attttttagg agaaaaagaa caacgtgaaa taatcgaaga
     1081 actaatgtta gtaaaaggat atcgcgatgc aaatgacaaa gcatttaagg ctttgaaaaa
     1141 aaatactgag aaaaaactcg gtaaaataga tgaaatcaaa tcgaagttac tgcaaacgaa
     1201 cggtggcgcc ctctcgtctt ctcaacaaaa attgcttgaa actttagtgg cattttcggt
     1261 agctgaagga ctaagtaaaa tggttgacca agaacttcag cagttgaaaa atatgttcaa
     1321 tcttatggat gagaaatttg aagcaaattg gaaagacgca caagaagcta gtgatattgt
     1381 aggtaaacat ctttcatacc cagaaaaggt aagtgcatta gataacggtg gtgtcaatga
     1441 aagtaaactt gctaccgaac cacataatga aataaaagac aaactaaata aaattacgga
     1501 cttatctaag aaatataatt cgtatttaca gcaaatcgaa aaaagtatta atgaaatagt
     1561 tgctaaggat caacaattgg cagggcaaat aggtgattta atatgatgtt tgaagaatac
     1621 aagaaaatag atgatcttga aaatgcatat gaaatagagt tgaaacgtat tgaaagggag
     1681 atacaaaatc ttagtgattt aaaatatcac cttagaagag aaaatgaaca aagttatgat
     1741 gcatttttgt atttaaagaa taaaatgaac tatagtgaag agtcaaatgc taaggtgaga
     1801 cgactagttg aagaatttga ttatgaagca gatacttata ttagacaaaa agagttaaag
     1861 ttagaagatt ataaagaaga aataagaaga gaatatatac agcaatctga aaaaataatg
     1921 gaggcgaagt agcttatggg aatccctgat ccgttatcta atcctatgtc tgacccttta
     1981 ctaggagata gttggatggt aggtgatttg aaaaaaagac aggaaaaaaa actaatcaca
     2041 acagtcatcg cacctgaaca tgatgaaaag catcgtgaaa ataaaagtaa aatgaaaaaa
     2101 tctgaaaagc aattgatcaa tcagttaatt aatcatggcc atacatttaa gagtgatttt
     2161 aaaaatgtag ctaaaggtga ttgggttaat aaagccatgc aagacttaga taaattaggc
     2221 aatgagctta aaaaaataat gaattagttg aattgtcaca tactttttca aaccaacaac
     2281 gaactaaaaa atccatataa atatgattaa aagaagtcat aaccattcgt aaaggatggt
     2341 tatttttaat agtaaacaaa attgctgcta acctacatct tgaaccaagt gaggataaat
     2401 aacctcctgc ctcccacacg attcaacacg aatttcaaat atattgctgt aaaataatgt
     2461 aaaataaacg tattcatatt actgaattga gggatagtat gcaacgtgat tatttaattc
     2521 gagtagaaac tgaaagcatg ccagatttca aaaggctcaa tggtttaatg attggttttg
     2581 ttattaaagg tgaggcacat atttatgatg aaaataacat gacgcaatgc aacagtggcg
     2641 acattttcat cattaaccac cgtgacttgt atcgatttca acttcaacaa gatggcatca
     2701 tatgttatat ccaattccaa atgaaatatt tggcagacaa gtttgatgat gcgcattgtc
     2761 tatattttca cttaacagat gcgaccacaa ccaagaatat acatcaactg agaaatataa
     2821 tggcaagact ggtttcaaca cacattcgac ataatgagtt gtctaaattg actgagcaac
     2881 aacttgtgat tcagttgctt atgcatatga ttcattatgt cccgcgtaca tatcattcga
     2941 accaaagtat cttaaatgat gataaagtga atcaagtatg cgactatatc gagttacatt
     3001 ttcatgaaga tttaagcctt tcagaattaa gcgaatacgt tgggtggtca gagagccatc
     3061 tgtctaaaaa gtttacagaa tcgctaggtg taggattcca acatttctta aatacgacgc
     3121 gaattgagca tgcgaaactc gatttaacat acacagatga aacgattact gatattgcat
     3181 tgcaaaatgg cttttcaagt gcagcgagct ttgcgagaac atttaaacac tttacgcatc
     3241 aaacgcctaa acaatatcga ggtgatcgtc cagcaatcac tgaaaatcaa caatcggcac
     3301 aacataatta tcacgaccgt gaattgatat tacttttaaa tgactacatt gaagaaatga
     3361 atcatttcat tgaagatatt gaaaagatga actataaaga gattgccttt aaaccaacta
     3421 atcaacaact aaatcaattt aatcatatta ttcaagtggg ctatttgagg aatttgctca
     3481 atacacagta tcaatcacag ttgcttacat gtcatcatga ttttcaagtc aatgaagtat
     3541 tagcatatga tgtgatgcca tatattatga aaaagctcaa tgcgccattc acgtatgatg
     3601 cagagatttc gaatatattt tatgatatcg atttgtgttt agacttttta ttagatcata
     3661 attttagtct aaccatgcat ttgaatcagt atgactcacg agattatatc gatgcattca
     3721 aagtatttat ccatcacgtt gccctgcatg tcagtcatag aaaagatttg aagttcaact
     3781 tgtatgtgac gacattgcac aatgctttga ttgaaatgat tgattatttt aaagcgttat
     3841 tccctaatgg tggcttgtac attcacttag atcaagctac ggaaagacat ctaccattgt
     3901 tgaaacgact tgagccacac atcgaccatt ttgtatttga tgccaattca aatgatgctg
     3961 ttgattttaa taaaatgaat gatgatgaat ttaaaaccgc gagtcaaatg attattaata
     4021 aaacgaatta ctttatcgac ttaatacatc gtcataacct aaagcgtcca ctcattttac
     4081 tcaattggaa tacattgacg ggtgatacat ttataaccaa cggcgaatat tttagaggtg
     4141 gtatcatcat tgagcagtta ttaaagttaa gctctaaagt agagggtatc gggtattggt
     4201 tgaattatga tttgcacgtt agtcattgta aaaatgaacg ggattatatg aattctattg
     4261 aactgtttca tcaatataat ggaaaacgtc cggtctattt cacggcattg ctatttaata
     4321 aattaacaag caatattttg tattctgatg atacatgtat tgtcacggga actgattcaa
     4381 attttcaaat attgttatat gatgcaaagc attttaatcc gtacttagcg ttggacaatc
     4441 aaatgaatat gcgtgcaacg gaaatgatcc atttgaacat taatgccttg gaagaaggta
     4501 tgtataagat taaacatttt accttagata aagaaaatgg tgcgttattt aatctttggc
     4561 gcaaacatca tacgatacat ggcatggaca aggactctat agattacgtt aatcgaatga
     4621 gttttccgaa attagaagta tatgatatag atatcacgga cacactggca ttaaacatta
     4681 aaatgattac gaatgggatt cacttaattg aagtaaaacg ttacccaagt tcataaaatg
     4741 atcacaaatc acaaattttg atatacataa tttgtgattt tttatattca aagctaaaat
     4801 tgcaaaaaat taatggttaa catctctgtt gttggcaata taaataatga ttaatcattt
     4861 atgatgtaac taaggagatg aaggatatga atcaacaatt aattgaaact ttaaaatcta
     4921 aagaaggcaa aatgattgag atcagacgtt atttacatca gcatccagaa ttatcttttc
     4981 atgaagatga aacggcgaaa tacatcgctg aattttacaa aggtaaagat gtggaagtag
     5041 aaacgaatat cggaccacgt ggaattaaag taacgattga ttcagggaaa cctggtaaaa
     5101 cattagcaat ccgtgcagac tttgatgcat tacctattac tgaagataca ggattatctt
     5161 ttgcatcaca aaataaaggt gttatgcacg catgtggtca cgatgcacat acagcataca
     5221 tgcttgtatt agcagagacg cttgctgaaa tgaaagatag ttttacagga aaagtcgttg
     5281 tgatacatca accagctgaa gaagtaccac caggtggtgc taaagcaatg attgaaaatg
     5341 gtgtattaga cggtgttgat catgtattag gtgtacacgt catgagcaca atgaaaacag
     5401 gtaatgtgta ttacagacct ggttatgttc aaacaggacg cgcatttttc aaattgaaag
     5461 ttcaaggtaa aggtggtcat ggttcatcac cccatatggc caatgatgcc attgttgcag
     5521 gtagctactt cgtcacagcg ttacaaacag ttgtatctag acgactaagt ccatttgaaa
     5581 ccggtgttgt cacaatcggt tcatttgacg gtaaaggtca attcaatgtc attaaagatg
     5641 ttgttgaaat tgaaggtgat gtacgtggat taacagatgc tacaaaagca acaattgaaa
     5701 cagaaattaa acgtttatca aaaggattag aagcattgta tggtgtaact tgcacattag
     5761 aatataacga tgattatcct gcattatata atgatccaga gtttactgag tacgtggcta
     5821 agacgttaaa agaagcaaac cttgattttg gtgtcgaaat atgtgaacca caaccacctt
     5881 cagaagactt tgcatactat gctaaagaac gtccaagtgc ctttatttat acaggtgcag
     5941 ctgtggaaga tggtgaaatt tacccacatc atcatcctaa atttaacatt tcagaaaaat
     6001 cattacttat ttcggcagaa gctgtaggga cagttgtttt agattacctt aaaggagata
     6061 actaacatga atgaaacgta tcgcgggggc aacaagttaa tcttaggtat tgtattaggt
     6121 gttattacat tttggttgtt tgcacaatca cttgtaaatg ttgtaccaaa tttacaacaa
     6181 agttttggtg cagacatggg aacaattagt attgcggtaa gtctaaccgc actattttca
     6241 ggcatgtttg ttgttggagc aggcggccta gcagataaaa ttgggcgcgt gaaaatgacg
     6301 aatatcggtt tattgttaag tattattggt tcagcattaa ttattattac gaatttaccg
     6361 gcattattaa ttttaggtcg tattatacaa ggcgtatcag cagcgtgtat tatgccttcc
     6421 acattggcca ttatgaaaac ttattatgag ggtgctgaac gtcagcgcgc cttaagctac
     6481 tggtctatcg gttcttgggg tggaagtggt atctgttcac tcttcggtgg ggcagttgcg
     6541 acaactatgg gttggagatg gattttcatc ttctcaatta tcgttgccgt actttcaatg
     6601 ttactcatca aagggacgcc tgaaacgaaa tcagaagtta ccaatacaca taaatttgac
     6661 gttgcagggc taattgttct agtagttatg ttgctaagtt taaacgttgt cattactaaa
     6721 ggtgcagcac ttggttacac atcattatgg ttctttggtt taattgcaat cgtaattgta
     6781 gcattcttta ttttcttaaa agttgagaaa aaagtagata atccgcttat tgattttaaa
     6841 ttatttgaaa ataaaccata tacaggtgca acgatttcga acttcttatt aaacggtgtt
     6901 gcaggtacat taattgtagc gaatacattc gtgcaacaag gtttaggtta tacagcattg
     6961 caggcaggat acttatcaat tacttattta atcatggtgt tattgatgat tcgagttggt
     7021 gaaaaattat tacaaaaaat gggttctaag cgaccaatgt tattaggtac attcattgtg
     7081 gtcattggta ttgcacttat ttcattagta ttcttaccag gcatatttta tgttattagt
     7141 tgtgtcgtag gatatttatg tttcggacta ggcttaggta tttatgcaac accttctaca
     7201 gatacagcta tttcgaatgc accgttagat aaagttggcg ttgcttcagg tatttataaa
     7261 atggcttcat cacttggtgg tgcattcggt gtcgcaatta gtggtgcagt atatgccggt
     7321 gcagttgctg caactagcat tcatacaggt gcgatgattg cactttgggt taacgtatta
     7381 atgggaatca tggcatttat cgcaatttta ttcgcgattc ctaatgatga taaacgtgtc
     7441 aaagatgcga aataataacg atgacacgcc aagtgttttg ataatgatat aaatgaaagt
     7501 aaagcaaatt aaatgaggaa atgtatagag cagtgcatat agataaatct atgattggat
     7561 taacactaat ataaatataa atgaaaaacc atccacttaa acttttgata agttaggtgg
     7621 atggttattt attgtgttgt tctaaaaatt tggacattta tatagaagga actatatcta
     7681 atgtgaagat aatggctatt cgcttcaatg aaggcgacgt gtaaaagtgt atattaaacg
     7741 tttcggggtc agactccgag aacagtgtcc gtcatgctta ccgcatacta atgaagcatt
     7801 attatatcag tgtttatagc cgatttaaga taataaattt tgctaaacaa aaaagccaac
     7861 ccatgaatgt tggattggct ttttacatgc atctgaatct ctaattttaa aaaaatatga
     7921 atataaataa gacagtaaaa attaaatttc agttgttgca atttcttcat ctgtaggtac
     7981 atcatcgtta aggccaacaa gtgcttcaga aacatttcgt gaatgataac cgatacgttc
     8041 aagaacacca atcatatcga tatatagtaa tccgcctttt gttgtacatt caccacgatt
     8101 aaggcgttta atatgacctt tgcgtagttt atgttcaata ttaaatgatt ctctactacg
     8161 ttctacaatt tcatcttttt tcgttttgtc ataaacatct aacatgtcga tggctttatc
     8221 aaatgactca gcaacatggt tgaataattt atccataccg cgttgtgcat cttctgtaat
     8281 gcgaatatct tcatcatgtt ggcgttttaa ttgagcgaca tactcttctg ttagctctgc
     8341 tacttttaaa atagagcgat tgacatcaaa cataactgct aaacgctcaa cgtcagcctt
     8401 cgttatggct tttgtagaaa ttctaactaa ataatttcga atgctatcat tgattgtttc
     8461 aacagcttgg tgcttttgtt caagcttttt gatcaatttt ttatcgtctt ttgtaatttc
     8521 gcgaatgtct tcaaacattg ataagacaat ctgaccaaca ttttgtaatt ctttttgagt
     8581 ttcttgtaat gcaacaccag gtgcgtgata aacaagatct ttgtttaagt gctgaggttt
     8641 atagtcatca gcaatatctt tacctgggac aagctttgta acaatccatg ctaaacctgc
     8701 tacaaatggt aattgaatca aagtatttgt tatgttgaag ataccatgtg atactgcaat
     8761 cgtcatcgct ggttttaagt gccatacatc ttgtaacaaa ctaatcaaat gaatcacaac
     8821 tggcaagaaa attgtgaaga taattacccc gattaagtta aagatgacgt gtacaagcgc
     8881 cgcacgtttt gcagcgattg agccggctaa actagctaag atagctgtaa tcgttgtacc
     8941 aatattatcg cctagtaaca cagggattgc tgcgtttaag ctaattaaat cttgttgata
     9001 aaattcttgt aaaataccaa tcgtcgcact tgaactttga actagtgctg ttaaccctgc
     9061 gccgacaata acagcaagta ttggatttga tgacatatca agcattaatt gcttaaatcc
     9121 atctaatgat gctaaaggtt taacggcatc acccataaat tctagaccga agaatagaga
     9181 accgaaaccg aatagtatgc ggccaatgtt attaatttta gagcgtttaa agaaaaagat
     9241 taaaaatgca cctaatgcta aaattggcat tgcatattcg cctaaatcta taccgataat
     9301 aaatgcagtt accgttgttc cgatattagc acccattatc actccaatgg cttgtttcaa
     9361 tgtcataaat ccagctgtta ccagtccgat tgtgataact gtcgtacctg aactactttg
     9421 tattaaaata gttacaacga tacctgcaat aacacctaat actggatttg atgtaaattt
     9481 gtttaaaata tctcgtagcc tgtctcctgc tgatgcttga agcccgtctc ccatgatttt
     9541 taagccgtaa aggaaaatac ctaaaccacc taaaaaggag aaaatgactt ctgtaaccga
     9601 catttccatt atttcacctc aaataagctt tatatttaga ttatcgctta taattgtaaa
     9661 tttaatgtta agattaggta aaattattta acaatatatg ttatttgtat atgacttgta
     9721 aaatatcgtc acttattatg taaattttca gtgtgaaatg gcaggtttgc aatcacttgt
     9781 ttaacaaaat gatgcaatca atcatgtaat tatgtttcat caaaaaaatc atgtgagtgg
     9841 gataacgaaa taaagtttgt gatcatatca cttctatccc actccatgat ttgaaatcac
     9901 catatagaca gatttaatgt gcctcgttat aacaatttgt gttcttttaa taatgactca
     9961 atgtacgtac cttttatctt tttaaggaat cctgctaatg cgagtttttg cattttcgaa
    10021 tctttagtaa tctcgcgcaa atcttgatgg tcattcagtt cgtatatggc atccattaag
    10081 acgcgaagat caaatgtact attgatgacc tctggaatac cacgatctat atttagtaat
    10141 tgataaacag cttccatggc agtacgaacc gaatattctg ttgtaaatac agtatctcgc
    10201 tctgtttctg caaagttacc aataaatgct aaattttgtg attgatgcgg gacgactaaa
    10261 ggtctgtcgc cgatagcacg cgtcatgaaa taagaggtaa tatatggcat ataaacagga
    10321 atcgtattag atgcatgttt tgctaagtct tcaattttgt cagttggtac acctaagtga
    10381 tacagccatt cttgacatat ttcattacca ctacattctg tgattggctt tttaatataa
    10441 tcgccgttta catctgaata taaggcataa atccatgtag atatttcatt tttaggttgg
    10501 tctttaaact gttgctgacg attgattgta aaactgattt gccatgcaga atcattgatt
    10561 gtaataattc cgccagtaac cgttttgcct gcaagagggt cacgtttaca aatgctttct
    10621 attgtatcga taatatcttt attgtttgtt gtcgatgttg cagaaacaaa ccaactcttt
    10681 tgaggaatat tttggcaaaa cttatcagga ttaccaaatt caggactttg tcgcgctaaa
    10741 tttttccata gtgtccaact accacctaat tcgtcagttg gtggtgctgg tgtatcatta
    10801 tcaccatacg tagagctttc cgtaatacta ccgtttgtca caaagacaag atcatctaca
    10861 gtcagtttaa ttgactctgc tttgccatgt cggtgaatta atatttctcg ggcaattttt
    10921 tgacttgtcg taacatctac tttaatatct tctactttta caccgtattc aaattgaacc
    10981 ccatgcgatt ttaaatattc aaccataggt aatactaaag attcatattg attatattta
    11041 gtgaatttta aagctgaaaa gtctgcgaga ccaccaatat gatgaacgaa tcgcattaga
    11101 tagcgacgca tttccatagc agaatgccac ggttcaaatg caaacatcgt tttccagtaa
    11161 atccaaaagt ttgaattaaa gaagtcatcg gaaaatacat ctgttatttt gacatcatct
    11221 aaatcttctt cattcgttaa gcataaatct aaaatttctt taatcgcttt tttggtcaaa
    11281 gtgaagtctc catcggtaac taaacgttga ccctgttttt caataactcg acagcgagaa
    11341 tagttagggt cttctttgtt tagccaatag aactcatcta atacagacgc gttatcgatt
    11401 tctaatgaag ggatagatct gaataagtcc cacaaacatt caaagtggtt ctccatttca
    11461 cgaccacctc ggacaacata gccttttaaa ggcatatttt caccatcaag actaccacct
    11521 gctttaggta actcttctaa aatatgaatc ttcgaacctt ccatttgacc atcccttatt
    11581 aaaaaacaag ctgcagcaag tgaagctaga ccagatccga ttaagtaagc ggatttgttt
    11641 tctacatttt caggtttttt agggcgcgca aatgcttcat aatttccata actgtaatac
    11701 atatccacca agtcctttcg tattagaata cactcagact ataccccttt gtgaattgca
    11761 aatattagta tttatattgg taaatttgac gaaattagat catttttata tttttgattt
    11821 ttaaaatttt atcactcgaa tgtttttgga ttctgaggtg cgggacttgg aataattcga
    11881 atcattatgt ggcatgttaa acaggtaaat ataagctatg cacatttaac aagcggatac
    11941 tcctgacgca attatagtat cattacatat tgtttaatcg tttggggtaa tgtaattaaa
    12001 aggtaaaata ggcagagtgt tatacataaa atgtaaagaa agaggcgata gaatgtctaa
    12061 aatcaggtct tttacaatat taagtctact tatttactta gctatgatgt gctatacagt
    12121 agtgacctat tcaaaattac caaccaaagt acctattcat tataatttag caggggatgc
    12181 tgataacttt gctgataaat gggtgctgct tttgattaat agcgcattta tagtgatttg
    12241 gcttatattt ttcattgcag gtagatacta tgaacgattt gccaaatggt cacattataa
    12301 tcatacacca cgtgaaattc gagcgattaa attattttta agtacgttaa atttagagat
    12361 tatgagctat atgtctatct tcacagtatt agaaatttgg caaatacaac accatcatca
    12421 attcaattta ctatggttta atatgatatt tatcattatc attggtttga cgcttgtcat
    12481 attttgttta cttcctacaa ttcataaaat gagagattct caataaaaat ataaactgt
//
LOCUS       DBNENW010000073        12431 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNENW010000073 DBNENW010000000
VERSION     DBNENW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.073"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..1070)
                     /locus_tag="AC1LW8_001607"
     CDS             complement(<1..1070)
                     /locus_tag="AC1LW8_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIP4773438.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNRFNTEKINIDVEAST"
     gene            complement(1073..1750)
                     /locus_tag="AC1LW8_001608"
     CDS             complement(1073..1750)
                     /locus_tag="AC1LW8_001608"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HIP4773439.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDGLKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(1881..2987)
                     /gene="rpoD"
                     /locus_tag="AC1LW8_001609"
     CDS             complement(1881..2987)
                     /gene="rpoD"
                     /locus_tag="AC1LW8_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIP4773440.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(3211..5028)
                     /gene="dnaG"
                     /locus_tag="AC1LW8_001610"
     CDS             complement(3211..5028)
                     /gene="dnaG"
                     /locus_tag="AC1LW8_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIP4773441.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQF
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTLNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPDDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(5089..5907)
                     /locus_tag="AC1LW8_001611"
     CDS             complement(5089..5907)
                     /locus_tag="AC1LW8_001611"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIP4773442.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(5918..6541)
                     /locus_tag="AC1LW8_001612"
     CDS             complement(5918..6541)
                     /locus_tag="AC1LW8_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4773443.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6876..8267
                     /locus_tag="AC1LW8_001613"
     CDS             6876..8267
                     /locus_tag="AC1LW8_001613"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIP4773444.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(8418..9164)
                     /gene="recO"
                     /locus_tag="AC1LW8_001614"
     CDS             complement(8418..9164)
                     /gene="recO"
                     /locus_tag="AC1LW8_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIP4773445.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLFVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(9192..10091)
                     /gene="era"
                     /locus_tag="AC1LW8_001615"
     CDS             complement(9192..10091)
                     /gene="era"
                     /locus_tag="AC1LW8_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIP4773446.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(10092..10496)
                     /gene="cdd"
                     /locus_tag="AC1LW8_001616"
     CDS             complement(10092..10496)
                     /gene="cdd"
                     /locus_tag="AC1LW8_001616"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIP4773447.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(10507..10851)
                     /locus_tag="AC1LW8_001617"
     CDS             complement(10507..10851)
                     /locus_tag="AC1LW8_001617"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIP4773448.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(10854..11321)
                     /gene="ybeY"
                     /locus_tag="AC1LW8_001618"
     CDS             complement(10854..11321)
                     /gene="ybeY"
                     /locus_tag="AC1LW8_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIP4773449.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRG"
     gene            complement(11322..12269)
                     /locus_tag="AC1LW8_001619"
     CDS             complement(11322..12269)
                     /locus_tag="AC1LW8_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIP4773450.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         3874 a         2332 c         1684 g         4541 t
ORIGIN      
        1 gtagatgctt caacatctat atttattttt tctgtattga accgattcat tagtaacgtt
       61 tttaaacctt ctttcatcac atattcgctg taatgattaa tatcaattaa attcacacca
      121 tgaattttag catctaaggc atcatgatgt ttaatatcac ctgtaacaaa gacatctgcg
      181 ccttgttgga cagcttgata ttcatatcca atacctgaac caccaataat tgcaatacgt
      241 ttaattttct gattagactc accaacaaaa cggacacttg ggatatttaa tttagattta
      301 atatcagctg cgaaatcttc caatgtcatt tgattatcca cttctgccat aacgccaagt
      361 ccataaaggg atgtttgttt tatctcaata aaatcaaata ccggtgtttc atatggatgg
      421 tattgtttaa ttaattgctc agcccttgac ttttgatatg catctatcat aaattcaatt
      481 ttaacttcat ctacatattc aattttatca atttgtccta ttgttggatt agcttcacca
      541 actggtttga attgccctct tccttcactt tcaaagaaac aatattcata attaccttct
      601 tgcgctaatc cattttcact aagcttatct ttaaatggtc caacattatc cttaggtata
      661 tatgtttgaa ctttatagta tacatcttgt tgattattta ttattgaaat gttcttcaaa
      721 cccatcgcct tcgccaacat catattgaca ccatacggat ttacatctaa atttgtatgc
      781 atcgctatta aattaatgtc atgttgaatt agttttctaa tgatcaaacc ataaccatta
      841 gcttttaatg atgttacgcc tttaaagatt agaggatgat gactaataat agtattataa
      901 cctttttcga ttgcttcatt tactacttcc aacgtacagt ctaatgctgt taaaacacca
      961 gtaacttcaa catcttcatc acctattaac aatcctacat tatcccaaga ttcagcagta
     1021 ctaaatggca catgatgatc taacaatgtc attaaatcag ctattttcat tactataaca
     1081 ccctttcaat tacagcaatt tcgtcattaa tttgagctaa acgttgatga tgttgttcag
     1141 tattgagttt cgatttaata tgataaagtg cttctaactc tctttgccat ttttttataa
     1201 aatattcatt tttgttgttc agcaattttg gtccgaattt caatccatca gatgatagct
     1261 ctattaattg tgtagaatat tctgctacaa caatttcata aatatggcct ttttcttcca
     1321 ttattatttc atcaattatt tcataattca attgttgtaa tgtttgtctt aaattttcag
     1381 tttggatatt actttgtaaa atcaaccttg gatgttgact taacttatct tgcccatctt
     1441 ttaaaatttt agcaataagt ggtccgccca taccacatat tgtgatatta tcgattacgt
     1501 cctcaggttg aataacactt aagccatccc ctaaacgtac atcaattcta tctactaatt
     1561 ggtttgcagc tacatttttt acagcagctt gaaaagggcc ttgaataact tctccagcaa
     1621 taccgcattc gcataaatgg ttttgaattg catagattgg caaataagca tgatctgagc
     1681 caatatccgc gattgtacct tgttttaaaa atcgacttac cgtcgttaat cggttattta
     1741 acgaaatcat tttattctcc tttatgaaaa aagaggttga acattattca atgtaccaac
     1801 ctcactcaag ttaaatctta atctaaaaat aaataagcgc ttattcatgt cttggtatca
     1861 atcattaaaa ttgatataaa ttaatccata aagtctttca aacgtttact acgacttgga
     1921 tgtcttaatt ttctaagtgc ttttgcttca atttgtcgaa tacgttcacg tgtaacaccg
     1981 aaaactttac caacttcttc aagtgttctt gttctgccgt catcaagacc aaatcttaat
     2041 cgtaatacat tttcttctct atcagttaat gtatcaagca catcttctaa ttgctctttt
     2101 aataattcat aagcagcatg atctgaagga ctttgtgctt cctgatcctc aataaagtct
     2161 cctaaatgac tatcatcttc ttcaccaatt ggcgtttcta atgaaacagg ttcttgcgca
     2221 atttttaaaa tttcacgaac tttttctgct ggtaaatcca tttcttcacc aatttcttct
     2281 ggtgctggat ctcgacctaa gtcctgtaat aattgacgtt gaacacgaat taatttatta
     2341 attgtttcta ccatatgcac agggatacga atcgtacgtg cttggtcagc aattgcacga
     2401 gtgattgctt gtctaatcca ccatgttgca tatgttgaaa acttaaatcc tttgttaaag
     2461 tcaaattttt caacagcttt aataagaccc atattacctt cttggattaa atcaaggaat
     2521 aacataccac gacctacgta tcttttagca atacttacaa ctaaacgtaa gttcgcttct
     2581 gctagtcttg attttgctac ttcatcacct tgttcaatac gtttggctaa ttcgatttct
     2641 tcttgtgcac ttaataagtt aacacgccca atttctttaa ggtacatacg aactgggtca
     2701 tttattttaa cacctggagg ggcactaaga tcacttggat tcagtttctc gtcagtatct
     2761 gaactatctt tttcattaac tagtgaaata tcattatcat ttaattgatc aaagaaatca
     2821 tccatttgat cagagtcgat atcaaaattc tgaagttttt cagcaatttc ttcatgactt
     2881 aaatgaccct cttttttacc cttttcaatt aattgcttct taacatcttc taatgttaat
     2941 gtcggatcaa ttgtttgttt tttaatttta actgtgttat cagacatgaa acggcctccc
     3001 gattttaaat atgaacattc gaaatttatt caatattgct attttaaact aaattcttaa
     3061 ttaattcatc catattttta attttatttt acaaattgga actaatccca atatttattt
     3121 ttcaatagtg ttgtttaaat gcggtaacta tcatttaccc ttatacattt tgacataatc
     3181 attattcgta ttattcttta aaatcacatg ctacatgcgt tctttattct tagcaacaat
     3241 ttgctgtaaa tagtattttt gtaattctac atcgccaatc cttgtagctt cccttaattt
     3301 atgattcaat gactcaattg tttcttgtcc tttttcatta ataacattga cataatcatc
     3361 aatttcattt tcatatggtt cgtcattcaa attatattgt tctaagctaa ttagtgtttc
     3421 tctcaactca tttgaattaa catactgcac agcatcactg atattatatt gatcattttc
     3481 cgcataaaaa tcatgtaaga cttcgaatac atatttaaaa tgctgatttg tgaagttatc
     3541 cttatcaaca ctttcataat aatttaaaaa tgtatcttta tctctcatta aatgttttaa
     3601 aaatgctcgc tccgcttttt cttgacggct caaattgtca aattgtgcca taccaattgg
     3661 ctcaggttca atataaccgc catactcatc ataaccgcca tactcatcat ctggataata
     3721 attggctggt gcttgattga attgtatttc gttagctaat tgctcaggac taacattgaa
     3781 aaatggtgca acatcattta aagccttttg ttgcaaaatc gatgatttca taagcgaaat
     3841 atcatgactt agttctttca aataacgttc atatgaaagg tcattatgtg caatttcatc
     3901 ttttaatata ctcactttat aatgtgcaaa tgactttttg tcatttttta caaaagcagt
     3961 aaatgcgtcg ttgccatact taccaatgta ttcatccgga tccatgcctg atggcaattg
     4021 tataacaaat acatttagcc cttgctgtaa taaatgttga cctgttttaa gtgttgcttc
     4081 actacccgca aaatccccat caaacattaa tgttatattt aatgttaact ttcgtataaa
     4141 agtaatatgt tcatctgaca actgtgtccc cattgttgca acaacgtttt tcaagccagc
     4201 agtatcagat tttataacat ccataaaacc ttctagtaat acgatttcat ctaattttct
     4261 aattgattta cgcgctttat ctaagttgta taacaacttt cttttttgaa agataggtgt
     4321 ttcaggacta tttaggtatt ttggttcttg accggtatat gttcgacctg aatatccaac
     4381 aattcttcct tgcgcatttt tcaaaggaaa cataatacga tttcgaaatc tatcgtaata
     4441 actgaaattt tcttcgttac gtgataataa tccggcttca tatgctaatt caatatcgta
     4501 accctttttt tgaagaaaat catgacaaaa atgtgagcta tcgggtgcaa agccaatgcc
     4561 tcgctcttta ataagcgcat ctgtaaaacc acgttcttgt aagtatgtta atgcttgttc
     4621 gccttcgact gtctttgtta aagcgtaata ataaaattct tgtattaact catgcatttc
     4681 aatcatttgt aaatcatcag aagcaatttg aacatttgag ttaaattgtg ttgcctcaat
     4741 atctacagca acattaactc tatcacctaa ttctttaacc gcttcaacaa atgatatgtc
     4801 tttaatttct tgagtaaatt gaaaaacatt gccacctttt ttacaaccaa aacaatgaca
     4861 gatttgttta tcttcagaaa ctgtaaatga aggtgtcttt tcatcatgaa aaggacacaa
     4921 acctatataa ttgcgtcctc tcttttctag ttttacatat tcacttacca agtctaaaat
     4981 gtcggtttta tcttttattt cattaatgat cgattgatct attcgcaaat taaatcacct
     5041 attaagcatt agttatacta ttatacctaa tttttgacaa aaatgaaatc atttcgattt
     5101 attttgttca atataatgga ttatatcgtt tgctgtttct tcgattgctt tttgagaaac
     5161 atcaatgaca ggacatccaa tttcacttac gatttcttca aagtaattca attcttcttg
     5221 gattcgagct tctgttgcat aacgagctgt gtcacctagt cccaattgtt ttaatcgctc
     5281 ttttctaatg cgatttaatt tttcttcact tattttaagt gcgatacatt tctttggatc
     5341 aatatcatat aagccatctg gcggtgtcac ttctggtaca atcggtacat tcataacttt
     5401 gtaactttta tgcgctaaat actgggataa tggtgtcttt gaagttctcg aaataccaag
     5461 taaaacaata tcagctttag gtaatccttt aggatcttta ccatcatcgt atttaactgc
     5521 aaactctatc gcatcaattt tcttgaaata tgcatcatct aatctatgaa cgatacctgg
     5581 ctcattataa ggtttttctt caaccgaagc tgataataaa tccattaatg gccccatgat
     5641 atcgacagac ttcaattgga attctgctac tttctcactc atatattgct tcatttcagg
     5701 tttaataagt gtataaacaa tgatagcatt tgtatctttt gcaacttgaa tcacttcatc
     5761 aacatcttca aaagattcaa tatatggata tcttaataat tcatttttac attgcttagg
     5821 attgaattgt gaaatacctg ccctagcaac taactctgcc gtttcaccta tagaatctga
     5881 agctacgata attttaattt tttccattac ttctcaccta ttctttatat aatgctacta
     5941 ataacttagc tattgttgtt ttggaaattc ttccaattac ttcatacttt tgattatctt
     6001 tttttcttac aattggaatc gaatcaattt ctttttcaat cattctatct gctgcgtata
     6061 tgactaattc gctttcctct aaataagtga cattaggcat acgtgtcata tttacactga
     6121 taggtactgt atgaatatct gctccaatca ttgaagctct taataaatct tttcttgaac
     6181 acacaccaac aaaatcgtta tcttcattaa taataaataa tgtacttaca tcttctaaaa
     6241 aaattgtaca aatagcgtca taaactgttg tattctctct tagcacaaca ggttgagaca
     6301 tatagtcctt aacctcaaat tgtcgaagtt tttcattaaa aaacttacct tttgatttac
     6361 ctgaataata atatccaact cggggacgcg cttctaaaaa acctgacatt gttaatatcg
     6421 ctaaatctgg tctaagcgtt gctcttgtta aattcaactt atctgctatt tgttcaccag
     6481 taatgggtcc tttagtttta acaatttcga tgattcgttc ttgtctttga ctgagttcta
     6541 taggtcttca cccctttttt attcacttat tattataccg atatttcttt catgtcacaa
     6601 acacattaat tttacttgcc tttaaataat ctatcaatta gaataaacgt acagcgagtt
     6661 aaggatagtg taagcttaac aataagattg gcgcaacgaa tcattttaaa ataaaagcga
     6721 gtgactacac taatttgggt ggaaccgcgg gttaaatacg taacttattt tgtattatat
     6781 acaatttaaa atattaactc gtcccatgga agtaaagcat atttactatg ctgttatttt
     6841 ccatggggcg ttttcatgta tgaggagagg taattatggc aaaagatatg gatacaattg
     6901 tttcattagc aaaacacaga ggttttgtgt tccctggtag tgatatttac ggtggtttat
     6961 caaacacatg ggattatggt cctttaggtg ttgaattaaa gaataatgtt aaaaaagctt
     7021 ggtggcaaaa attcattaca caatcaccgt ttaacgttgg tatcgatgct gcaatcttaa
     7081 tgaatccaaa agtatgggaa gcttcaggac acttaaacaa cttcaacgac ccaatgattg
     7141 ataataaaga tagtaaaatt cgatatcgcg ctgataaatt aattgaagat tatatgcaag
     7201 atgttaaagg tgatgaaaac ttcattgccg atggtttaag ttttgaacaa atgaaaaaaa
     7261 ttattgacga tgaaggtatt gtttgtcctg taagtaaaac tgctaactgg actgaaattc
     7321 gccaattcaa tttaatgttt aaaacattcc aaggtgtaac tgaagattct acaaatgaaa
     7381 ttttcttacg tcctgaaaca gcacaaggta tttttgtaaa ctataaaaac gtgcaacgtt
     7441 caatgcgtaa aaaattacca tttggtatcg gtcaaattgg taaatcattc cgtaatgaaa
     7501 tcactccagg taacttcatt ttcagaacaa gagaatttga acaaatggaa cttgaattct
     7561 tctgtaaacc tggagaagaa atcgaatggc aaaattattg gaaaactttt gcaagtgact
     7621 ggttaacaag cttaaatatg agcagtgaaa atatgcgttt acgtgatcat gatgaagatg
     7681 aattatctca ttactcaaat gcaacaactg atattgaata taaattccca tttggttggg
     7741 gtgagttatg gggtatcgca agtcgtacag acttcgactt acgtaaacat gctgaacact
     7801 ctggtgaaga tttcagatac catgatccag aaacgaacga aaaatatatt ccatattgta
     7861 tcgagccatc acttggtgca gatcgtgtaa cattagcttt cttatgtgat gcatatgatg
     7921 aagaaggcgt tgaaggtagt aaagatgcac gtacagtttt acacttccat cctgcattag
     7981 caccatataa agcagcgatt ttacctttaa gtaagaaatt atctggcgaa gcgattaaga
     8041 tttttgagca attaagttct aaattctcaa tcgatttcga tgaatcacaa tctatcggta
     8101 aaagataccg tcgtcaagat gaaatcggta caccttattg tgtaacattc gactttgatt
     8161 cattagaaga taatcaagtt acagtacgtg acagagattc aatggaacaa gttcgtatgc
     8221 caatctcaga gttagaagct ttcttaactg aaaaaacaaa attctaatta aaaagactct
     8281 ccaccatatg tggtggctat ataatttata aaaagagata ctggttagta aaattgccag
     8341 tatctctttt ctattcgaaa aaggtattca aataaacaca tctatgtggc tacttgaata
     8401 ccttttatta ttttatgtta ttgttccaat ctttttaatt ggttgattag tttctgactt
     8461 ttaaaaaaca tacctgcata ttcacgatat aacattaaaa tgatatctga catttcatca
     8521 ataatttctt gatggatatt caatgaattc attttatcta tcggtaattt ttgtaataca
     8581 tctaataaat atagtgtttt attcgataat ataactgcat gtacatcttt agaagcctct
     8641 tgccttgaaa tcgcaccgtc aaacttaaaa ctataaccta ttaaatctgc ttgtgtgtca
     8701 ttaccactca cagcacagcg attaaatgat gcagtaaaac caaatcgttt catacacttt
     8761 aacataacta cgactgacat taactgtgca gatgtacctg attctatttt ttcaagaaca
     8821 aattgtaata attgatagtt atatggtgca atgtcacctt catccattga gcgctcaata
     8881 gtttcagctg ccagagacgc ataactgctt acgaaaaggt ccatttgtaa tttataatgt
     8941 tgactaataa catctacaga atttaacgtt cccatacctc gccactgatt gtaaataaac
     9001 aaaccataaa caaacaattg cgtttgcgct tgtaaacccg tcttaacttt tttagcacgc
     9061 ctagccataa gtggtacttt tgcaccatgc tcatttaaaa tcgtgataat tttatcagat
     9121 tcaccataat caactgcttt gatgataatc cctttttggc gcattaacaa ttatcttcac
     9181 cacttttaag attaatcttg gtcttcaaca taaccaattt ggcgaataaa gttaactttg
     9241 tttcgccagt ctctttgaac tttgacccat aattctaagt aaactttaga gcctagaagc
     9301 atttctatat cacgtctcgc acgtttccct acttctttta actttttacc gccttttcca
     9361 atgacaattc ctttttgcga atctctttca acatatatag ttgcttcgat atgaacacga
     9421 tcttcgcttt ctttaaccat acggtccaca ttaacaccaa tcgcatgagg gatttcttca
     9481 cttgtaagat gaaggatttt ttcacgaatg atttcaccca ctacaaattg ttcaggatgg
     9541 tctgaaattt gatcatctgg ataatattta ggtccttcgg gtaaatacgt ctttaaaaca
     9601 tcaataaaat gatctacatt tagcccttct aatgctgaaa taggtacaat ctctgtaaag
     9661 tccatataac tttgatattc ttcaatcttt ggcattaatt catctggatg cactaaatct
     9721 attttattta atactaaaaa tactggtgtc ttaacatttt tcaacatttc tataatatat
     9781 tcatcacctc gtccaatttc ctcattggca ttaaccataa acatgattgc atctatctca
     9841 gataatgtat ttttagcgac tttcatcata tagtcaccta atttgtgttt aggtttatga
     9901 atacctggcg tatcaatgaa tataatttgc gcgtcatctc ttgtcataac accttgaatt
     9961 ttatttctag ttgtttgagc tttatcggac atgattgcta ttttatggcc gatcactcta
    10021 ttaacaaatg ttgacttccc tacatttggt ctacctataa ttgaaacaaa tcctgattta
    10081 tgttctgtca tttattctaa atcctttcct gaaaatccaa atggtagtaa ctctgcgact
    10141 gtcatcataa ccatatctcc tttatgattt gtcatataca caggcatatc atcatcacat
    10201 aattccttta aaacttgacg acatgcacca caaggtgatg acggtttatc tgcatctacg
    10261 gttacagtta ttgattcaaa atcacctggt ctgtatcctt gagaaattgc cgataccaaa
    10321 ctagctcgtt cagcacatat cgataatgga taagaagcat tttctacatt ggtaccataa
    10381 aaagttctac cgtctttcgt ttttaaataa gctcctactt taaattgact gtatggcgaa
    10441 tatgattctt gttgtgcttt tctaacttct tgaaaataat gaggttgata actcatatat
    10501 gcctctctaa aataacgcta taaaatgtgg taaaaatact attaaaccta taataaatgc
    10561 taatattgaa actataagta cactaaaagc cgctatatct tttgcgtatt tagctaaatc
    10621 atgatattca acggtcacta aatcgacaac atattcaata gcagtgttta aagcttcaac
    10681 agtgagaact aatgcaatag caatgagtat aaatatccac tcaatacgat taatatttag
    10741 tacgagacca aagacaatag caacaatcat tgcaaacaca tgtaaaagaa atttatagtc
    10801 tttttgaatt aagattttca gcccatcaag tgcatattta aaccttttca taattagcct
    10861 cgtgttaatc catatgcgtt taatattgta tcttgtcgac caaacatttc cttttcatcc
    10921 gcttcagtca tatgatcata acctaataga tgcaaaaatc catgtaatgc taaaaatcct
    10981 aattctcgtt caaaagaatg tccgtaattg tttgcttgtt cttgcgctac atccgtacag
    11041 ataattatat cccctaaaac acgtggtata tcaagaccac taaaatcaat ctctggctca
    11101 tcttcttcta aagcaaatga gattacatct gtaactttat ctttatctct atatgttcga
    11161 ttaatttctt gtatttcttg tttatctaca aatgtaacag aaagctcagc atcgtcttct
    11221 atatgctctt cttttttagc aaattctaat aaatcttcaa tttgtttata ccacgcatct
    11281 ttaactaagc ctgtgtgatc gctaaaatct atcgtaaaca tttaattctc tccttcataa
    11341 tgttcaatga tcttacttac caatggatgt cttactacat cgctctgatc taatttcaat
    11401 atacttatac ctttaacgtt gtgtaaccta ctgaccgctt ccttaagtcc acttttaaca
    11461 cctttaggta aatcgatttg agtttggtca ccagtaacta ccatttttga gccaaaacct
    11521 agtcttgtta aaaacatttt catttgcgca tgtgtcgtat tctgcgcctc atcaagaatt
    11581 acaaatgcat cttctaatgt tcgtccgcgc atatatgcaa gtggcgctat ttcgataatg
    11641 cctctttcaa taaatcgctc tgtttgttca cgcccaagaa cagtatatag accatcatat
    11701 aaaggtctta aatatggatc taccttttct ttcaaatctc ctggtaaaaa tccaagtgac
    11761 tctcctgctt caacagcagg tcttgttaat acaatacgtt taacagcacc tttacggagt
    11821 tgctttgctg cataaactac agctaagaat gtcttacctg taccagcagg acctatacca
    11881 aatactaaat cattattttt catggcatta acatatatac gttgccccat cgttttcgca
    11941 cgaatcgtct taccaaatgc atctttagtt atctcttcat catataaatc taacagatgt
    12001 tgaattgtgt tattatgcgc cattttaata gctgcttcaa catctttaat tgtaatatta
    12061 ttacctaaat caataacctt cagcaaattg attaatactg attccgcttt ttctacgttt
    12121 tctatttttg tacctttaac ggcaacttct tgtcctcttg catggatgac aacatcgaaa
    12181 ctctcttcaa ttgcttttaa atgttcatca ttatttccaa ttaaagcttg agattgattc
    12241 atatcgtcta tttgtataat tccaggcata cacgcgctcc ttttcatata tcatttatat
    12301 tcattctatg atgctttaat atctacttaa ttatatcatg caattgattg atttaaacat
    12361 ctttgcattg acttactcat ttttctttaa tttaaaataa tcatttatac ttttcacctt
    12421 gtcataaaag c
//
LOCUS       DBNENW010000074        12421 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNENW010000074 DBNENW010000000
VERSION     DBNENW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.074"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..355)
                     /locus_tag="AC1LW8_001620"
     CDS             complement(<1..355)
                     /locus_tag="AC1LW8_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773451.1"
                     /translation="MNEQIIGSIYTLAGGVVLYSVKEIFRYFTDSNLQRKKINLEQIY
                     PIYLDCFKKAKKMIGAYIIPTEQHEFLDFFDIEVFNNLDKQSKKAYENVIGFRQMINL
                     SNRVKAMEDFKMSFNN"
     gene            complement(514..663)
                     /gene="rinB"
                     /locus_tag="AC1LW8_001621"
     CDS             complement(514..663)
                     /gene="rinB"
                     /locus_tag="AC1LW8_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HIP4773452.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            complement(707..991)
                     /locus_tag="AC1LW8_001622"
                     /pseudo
     CDS             complement(707..991)
                     /locus_tag="AC1LW8_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SA1788 family PVL leukocidin-associated protein"
     gene            complement(1004..1408)
                     /locus_tag="AC1LW8_001623"
     CDS             complement(1004..1408)
                     /locus_tag="AC1LW8_001623"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HIP4773453.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYVHTYMPTNYTKHKKYL
                     QSQMPRLNLENALKIELAFYFTPPESWSKKKKAQAIGQLKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            complement(1417..1635)
                     /locus_tag="AC1LW8_001624"
     CDS             complement(1417..1635)
                     /locus_tag="AC1LW8_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773454.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKFNGRRW
                     YLDDYELNTLIDNEQMELGHQTNLFEYI"
     gene            complement(1642..2535)
                     /locus_tag="AC1LW8_001625"
     CDS             complement(1642..2535)
                     /locus_tag="AC1LW8_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIP4773455.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KAAAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQIESKKQTYKPHVKQSKEKTPKWLTDGTRETKTAE
                     VDENLEKDREAFIKRLNSKWE"
     gene            complement(2565..3035)
                     /gene="ssb"
                     /locus_tag="AC1LW8_001626"
     CDS             complement(2565..3035)
                     /gene="ssb"
                     /locus_tag="AC1LW8_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP4773456.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNTDDNQQDLYQQQAQQTRGQSQYSNNKPVKDNPFANANCPIEIDDNDLPF"
     gene            complement(3036..3653)
                     /locus_tag="AC1LW8_001627"
     CDS             complement(3036..3653)
                     /locus_tag="AC1LW8_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP4773457.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKIL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSALANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKREIQKVTGVPVYVGGL"
     gene            complement(3734..4654)
                     /locus_tag="AC1LW8_001628"
     CDS             complement(3734..4654)
                     /locus_tag="AC1LW8_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HIP4773458.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            complement(4656..6611)
                     /locus_tag="AC1LW8_001629"
     CDS             complement(4656..6611)
                     /locus_tag="AC1LW8_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP4773459.1"
                     /translation="MMMDIKINKLTISNFAGIKEESFNFDGKDTKIYGNNATGKTTTA
                     TALQWLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPK
                     YTINQKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLD
                     WKKRRSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIK
                     DIPIRINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGAEEINLRNQLADKQ
                     SELKRIEDNNSASNENKIHTLTNELHVENGTVANLKTRLKQNKQQIAHEENRRNQLLE
                     NHKGLKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVNEAREKALQKFNASKSKELET
                     IQTSINHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVT
                     QTDEYKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNK
                     HLDDVISELRNEEDRLLDEKEKYSHNLYILKEFTTTKVKMLTENINNEFDIAEFKLFN
                     TLVNGELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESV
                     TELIITESQQIQLIVNEQDKKLRMETI"
     gene            complement(6608..6871)
                     /locus_tag="AC1LW8_001630"
     CDS             complement(6608..6871)
                     /locus_tag="AC1LW8_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773460.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            complement(6880..7140)
                     /locus_tag="AC1LW8_001631"
     CDS             complement(6880..7140)
                     /locus_tag="AC1LW8_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HIP4773461.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     GINITDENDLYTALDILNQSIYEWIEENTDERDRLINLVMRW"
     gene            complement(7233..7394)
                     /locus_tag="AC1LW8_001632"
     CDS             complement(7233..7394)
                     /locus_tag="AC1LW8_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HIP4773462.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(7391..7711)
                     /locus_tag="AC1LW8_001633"
     CDS             complement(7391..7711)
                     /locus_tag="AC1LW8_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HIP4773463.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            7770..8402
                     /locus_tag="AC1LW8_001634"
     CDS             7770..8402
                     /locus_tag="AC1LW8_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773464.1"
                     /translation="MYIDPLKNVRFSINNVISNVEISKSMAIKQSLKPKYQLDIINRN
                     NINLFSDFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEETIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQSTYKRHDEFVKRIENDFPHPFKKLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            complement(8417..8557)
                     /locus_tag="AC1LW8_001635"
     CDS             complement(8417..8557)
                     /locus_tag="AC1LW8_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773465.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(8588..8785)
                     /locus_tag="AC1LW8_001636"
     CDS             complement(8588..8785)
                     /locus_tag="AC1LW8_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773466.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRPVNIQYNDGYDLMIDPRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            complement(8801..9550)
                     /locus_tag="AC1LW8_001637"
     CDS             complement(8801..9550)
                     /locus_tag="AC1LW8_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HIP4773467.1"
                     /translation="MQALKTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            9607..10146
                     /locus_tag="AC1LW8_001638"
     CDS             9607..10146
                     /locus_tag="AC1LW8_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773468.1"
                     /translation="MDIIAICIAIFSFLLTALKYYLDYMKDSLNIDVIPTRSFNYLVD
                     DKSSYNDITFINFTKFPISVIDVEFDIKNKVNEQKTFKPIRYKDKNYSIPFTLGPYES
                     VECTFLLEEYPVIWEWDVTIKVTTNKGIYIKPVIIESRTEHRESEPQVTELTSANKVS
                     ALSNPKDGFLKKFLYHLKP"
     gene            complement(10170..10430)
                     /locus_tag="AC1LW8_001639"
     CDS             complement(10170..10430)
                     /locus_tag="AC1LW8_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP4773469.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDEKINATPIKE"
     gene            complement(10448..10672)
                     /locus_tag="AC1LW8_001640"
     CDS             complement(10448..10672)
                     /locus_tag="AC1LW8_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HIP4773470.1"
                     /translation="MCYDYSRLSGKIVEKYGTQYNFAIAMKLSERSLSLKLNGKVGWK
                     DSEIWKAIQLLDIPVEKIHLYFFKEKVHVI"
     gene            10831..11601
                     /locus_tag="AC1LW8_001641"
     CDS             10831..11601
                     /locus_tag="AC1LW8_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIP4773471.1"
                     /translation="MREKVSNRLKHIMKIRNLKQVDIINKSKPYQKKLGISLSKSTLS
                     QYINDVQSPDQDRIYLLSKTLNVGEAWLMGYDVDSYRVPDEERQDETIMSKINNIFSQ
                     LTPPRQENVLNYANEQLEEQNKVTSIDGYKESKLVSYIACGATGAGIGEELYDDILHE
                     EVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLI
                     KRVDICEDYINLVSLNPKYDDIKVASFSDIKVMGKVVL"
     gene            11797..>12421
                     /gene="seq"
                     /locus_tag="AC1LW8_001642"
     CDS             11797..>12421
                     /gene="seq"
                     /locus_tag="AC1LW8_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HIP4773472.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYANYQPEK
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDKVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYKSKFNSGFNKGKITFHLNNEPSFTYDLFYTG"
BASE COUNT         3567 a         2163 c         1829 g         4862 t
ORIGIN      
        1 cattgttgaa actcatctta aaatcttcca ttgccttaac tctatttgat aaattaatca
       61 tttgtctaaa tccaataaca ttttcatacg cttttttact ttgcttatct aaattattaa
      121 agacttcaat atcaaaaaaa tctaaaaatt catgctgttc tgttggaata atataagctc
      181 caatcatctt tttagccttt ttaaaacaat ctaaatatat cggatatatt tgttctaaat
      241 tgattttttt acgttgtaag ttagaatctg taaaatacct aaaaatctct ttaactgaat
      301 aaagcacaac acctcctgct aaagtatata tgcttcctat tatttgctcg ttcattttta
      361 tctacctctt tataatattt tctgaaaagg aatctataat tttatacata ccaaaaattc
      421 ctaatgcatt tattatcact ccatcatcaa taatatatat ggatattaag aaagcaaaca
      481 gcaaaacgat caaatcataa ataaatattc tcattattca ctcacctccg ctcgaaagac
      541 gtaatcactc ggcgcctcta catcatcatt agccgtcatc ataatatata cttgctcagt
      601 tacatactta cctaactcgt acattgctag taggaataat aatcttagta tttgcttaat
      661 catttcccac actcccttat attttcaaac aactgaccca ctttaataac tgcacctctt
      721 ttaacttgcg cctcgtacct ctctttcgct ttctttccaa tttgcgttcg agtctagctt
      781 gttccagtct ttctattgtt ttcttttctc tgtactcgct taaacgcgta ccttctggtg
      841 cgtccattgc ttcatgtagt tcccaaccgt cttttactct cttagaaacc attccagcgt
      901 ttataccgtg actttctatt aattccattt caaatttact gaacctataa ggtttatcat
      961 ttattgttac aatccttgct tttctcgcca ttttatccac ctcttatatt tcttctattc
     1021 gtatgattat tttgggctca attccataac gctttgagct agttatttct gtaatttggt
     1081 tatcgtcttt ccatacatgg ccattacaag catctaatac cgttttaatt aagttgtcga
     1141 tatccggctt agtcactttt aattgtccaa tcgcttgagc tttctttttc ttcgaccatg
     1201 attcaggtgg agtaaagtaa aacgctaatt cgatttttaa tgcattttct agatttagcc
     1261 ttggcatttg actttgtaaa tattttttat gttttgtata gtttgtaggc atgtatgtgt
     1321 gtacgtatct gccttttgtg ctaaaacgcg gtcgaggcga ccccatcggc gcattaaaca
     1381 cttcgttaaa tttaatttct atctccatgc aatccctcat atatattcaa ataagtttgt
     1441 ttggtgtcct aactccattt gttcattatc aataagtgta tttaattcat aatcgtctaa
     1501 ataccaacga cgaccattaa attttgtttc ttttattcca acaactaaat gccgaccatc
     1561 tttaaaatgt ggtgtaactg aaaacatttt gttgccgtca tgatcaaata gatagtattt
     1621 atcaaatgca tccattttca atcactccca tttgctattt agacgcttaa taaaagcttc
     1681 tctgtctttc tcaaggtttt catctacttc cgccgttttc gtttctctcg tgccgtctgt
     1741 gagccattta ggcgtttttt cttttgattg tttaacatga ggtttgtatg tttgcttttt
     1801 gctttcaatt tgttgctttt caaatgcacg tacttgttcg atagatttca agtttgcatt
     1861 aagccatgta ttcaaaatgc ttttagcata tccccaagta actttgtttc tgtctttagc
     1921 gattttaagt gatgccgtaa ctatttcatc tgaatcattt tcaaatgaat caagataata
     1981 atttaaatcg tctaaattgt atggagttat gaaaccgaat ccgttatctt ggaagaagtc
     2041 gaaggcagct gccttcttct tctcattatt cacattcttt tcattattat ctttattatc
     2101 attattgttt gtgttggttt gatgttgttt tgatgttgga ttgatgtttg actgatgttg
     2161 ttttgatgtt ggtttgatgt cgttttgatg ttggttcctt ccctgctcac tttgataaaa
     2221 gtcataattg acaatggtta taagggtata ttttgatgtt gttttgactt ctaacattcc
     2281 atcactctcg agtaagtcaa ggaaggtttt cactttaaat cgtgaccagt taaaaaggtc
     2341 agacaaggtc aaaatcgatg ttaatctttg tcctctttct acggttacaa tttggtttcc
     2401 aataggcact tttgcctttg aatgattcgc ttccatgagt aaatatatcc atgcttcaaa
     2461 ctttgaaaat gttctctttt ctttaaatag ccaatgattt tgaattgagc gatcaatact
     2521 tatccaacca gtcatataca cacctcactt tcaaaccggt taaattagaa tggtaaatca
     2581 ttgtcatcta tttcaatcgg acaatttgca ttcgcaaacg gattatcttt tactggtttg
     2641 ttatttgaat attgcgattg tccacgtgtt tgttgcgctt gttgttggta taaatcttgt
     2701 tgattatcat ctgtgttctt cggttctaaa aattgaatac tatcggcaac aacttccgta
     2761 acgtatacac gttgaccttc cttattttca tagttccgcg tttgtaacct accatctacg
     2821 cccgccaacg atcctttaga taggtattta ttaacgttct ctgcttgttt tttaaatacg
     2881 atgatattaa taaagtctgc ctcgcgctct ccttgtgcat tcgtaaatgt gcggtttact
     2941 gctaatgtga atgatgctac atttacacca ctttgagtgg ttcttaattc tgggtctcta
     3001 gttaaacgac caactaatat tgttctgttt agcatttata aacctccaac ataaacgggc
     3061 acgcccgtca ctttttgtat ttctctttta atgtattttg catttgaatt ttggctactt
     3121 aaatgaatta aatgtatttc ttcgagtcta gttaaatcat ttgctttcaa cattcctata
     3181 gcatgttcta agctaaaatg agactccata attctatttg ctaatgcgct gtgtacactg
     3241 ccgtttttta tgttttcttg catttgttca tagatataat taacttctaa catcatgtgt
     3301 gtaatgccgt taaatttgta tttcagatac ttcgtatcag taacatacag gatcttataa
     3361 cctagtgtgc tttgtaataa gaaagccaca ggctcgttag catcatgttc gatgtcaaac
     3421 ggtagaattg accatgtacc tattcgcagc tcttgctttg ccttaatcgt gcataagcga
     3481 tgactttcaa aattcatagc ttgttgtgtt ccagcagtca tatagctgat tacaccattg
     3541 tcgacaaact gctttgtata ctttgcatga tcaccatgtt cgtgtgtgat aagacaccct
     3601 gctatatgtc ttgttttata tttgaaatgc ttttgaacac gttcaaattt tatacctgcc
     3661 tcaagtagta acgtagtacg tccatcattt aagacgtagc agttaccact tgaaccagtt
     3721 gctattgttt caattaaaat ggctcttctt cgctttcttt ttctgttgca ggttctttta
     3781 tttcttcaaa gtcagataca tcaataggct tatcattttc taattctgtg tattgtgctt
     3841 cttcaagaac tggttgttca aagtccaatt gttcttgatt tgcattttct tcaacttctg
     3901 cgtccaacac ttctttgcgt tgacgttgtt cggattcttg tgcgtatttg aaaagattgc
     3961 tatctgttga tgtgttgata taacgtttag cagctctatt gataactgtt tttttagcca
     4021 tttcttcttt gaaattatta tgtgttttag aattttgtaa tgctttttca tctttaatca
     4081 ttgatgactg catccatgct tgtttaattt gttcaatagt catgacttca atatagttat
     4141 ctcgtccatc attaaatacg attgtgcagt acgcaccgat aatgttttct ttgtcgatgt
     4201 taaagaagtc ttgttcgtgc ttaatcgctt tgatacgtcc tgtttctccc atttcttgct
     4261 tgaatgtatc gcctttataa atcacttgag caacaacatc ttgagcacct gcatcacgtt
     4321 ttaacatcat tacattaccg tgatagctac gttgtaactg cattttgttg ccgtaaggaa
     4381 taaagtagca ttgattttta gctggattta aaccttgcgt taccatgtct aataaggcat
     4441 ttgctttgct tgtatcgtta caactcatta atttgttatc ttggctgatt tgtaaccatg
     4501 cttgtttcat ggcattactt ggtgaataat catttggcaa ttccaaattg ccttgtgact
     4561 ctaaaactct cactttgttt aatacgttgt cagatacgtt cttttcttgt actaattgtt
     4621 gttcaatagt ttgtaattta ttattttcag tcattttata tagtctccat tcttaatttt
     4681 ttatcttgtt catttactat caattgaatt tgttgtgatt ctgttattat aagctctgtt
     4741 actgattcag cattatcaat aaatattggc gctgtaactt taaaatgttt tgacagtgta
     4801 ttgatgatat ctaagccaac attaattctt gaggcgttat ttaaaccgct gtcgtattcg
     4861 acgccgttaa ccgttgttga acatgtttct tctaattcgc cgttaactaa ggtattgaat
     4921 agcttaaatt cagcaatatc aaattcgtta ttgatatttt cagtaagcat tttgactttt
     4981 gttgttgtaa attcttttaa gatataaagg ttatgtgaat acttttcttt ttcatccaat
     5041 aatctgtctt cttcatttct taattcagaa ataacatcat ctagatgttt atttgatttt
     5101 tcgattgatc ttgacacttc aatttctgat ttttcttgag taagttcgct tattttgtca
     5161 tctattcctg aaactttatc ttgaatagtt ttcctgatgt ttgagcgttt ttgattaatt
     5221 tcatttatct ctaacattac tgctttgtat tcgtcagttt gtgtaacgtc aacgtgagtc
     5281 gtttttaact tattaatttt gttttgtatt cttgctgaac gctcttctgc ttcgttgatt
     5341 ttaatttgaa gattattatt gtcatcctct aacttctcga tgattggctt tattttcttg
     5401 ccttctgaaa taatgtgatt gatagatgtt tgtattgttt ctaattcttt cgatttgcta
     5461 gcattgaatt tctgcaatgc tttttctctt gcctcattca cttgttcagc tggtaactgt
     5521 tgaccacaac aactacatac attgtcatca agatattcaa atttttgatt tttagctttt
     5581 tctaaatcac tttttaatcc tttatgattt tctaataatt gattacgtct attttcttca
     5641 tgtgcgattt gttgtttgtt ttgctttaat cttgttttaa ggttcgcaac cgttccattt
     5701 tcaacgtgta actcatttgt taaagtatgg attttgttct cattacttgc gctgttattg
     5761 tcttcaatgc gcttcaattc tgattgttta tcagctaatt gattacgcaa attaatttct
     5821 tctgcaccgt tttgaatatc tatacgctca ttttcaagtt gctcaatttc ttgtttgata
     5881 attgcgtatc tatcattatc gaattcaggt acatcctgct tattttgttg tgtttggtta
     5941 atacgtatcg gaatatcttt gatatctttg ttaatctgtt ttatcttgtc cgtaagaatc
     6001 tttttctttg tttcaatttc atgatctcca agaatattat ttaattcttt aaaatcatca
     6061 tttgttttaa tgacatcctc atcattgatt ggtttagcaa tttcaaacaa caaacttctt
     6121 cgcttcttcc aatctagtaa gttaaatgct tgagggttcg taattaactt gaatacatct
     6181 tcatcaatca gttcatcaat acgagcttta taatccttta cttttattga ttcatcattg
     6241 atatattgtt tcttcgttcg acttcgtgag tattccttgc gattcgtttt ttgatttatt
     6301 gtgtatttag gatgtgactc ttttttgaaa gtcgttattt ttccgtcgat ttcaaattct
     6361 gcgaaaacag tcggaattaa ctcataattt tcttcgtttt tttcgtttaa aggtacaggg
     6421 ttaaatgatt tggttgatcc gtccaaacct ttatcgaaca gcagccattg taatgcggtt
     6481 gcagtcgtag tcttaccagt cgcattattg ccgtatattt ttgtgtcttt accgtcaaag
     6541 ttaaagcttt cttctttgat tccagcaaag tttgatattg ttagcttatt tatttttata
     6601 tccatcatca tgctcctttt ttaatctttc ggtgacctct taacacctcg ataattaaat
     6661 tttttattcg ttcgtggctg tccggattga tttcatgtat ctgtacaagc ttattgttcg
     6721 ttttgtaact gtcgtgatag tgcaagaaat taatcgataa gtatccgtga tgattacgtt
     6781 caatttccaa taatgctcgt tggtttgaca aagtatattc gtcgaataac gttttaaaaa
     6841 tattcaatat atttctttct gtatctctca tgcttatacc taccatctca tgactaagtt
     6901 aattagcctg tctctttcgt ctgtgttctc ttcaatccat tcataaatag attgatttaa
     6961 tatgtctaat gctgtgtata gatcattctc atctgttata tttataccgt cgataaatct
     7021 atcttctaaa tctaagacat tcactagaat gctgtaatct tgtttcttaa ctgctaattt
     7081 aaaatcgaat ccgtctacat taattacttt ttgacataca tcgccaattt tgtagtacat
     7141 tgttgacact tcctttattt cgttttatat tgaatatgca ttaattttct aattgtttag
     7201 actgttactc attgccgtga gtaacagttt ttttattctt cataaaagta ttccttataa
     7261 aatatgaatg ttgcgatact tgcgaatccc gcaatcgacc atgcagtagt gaagtataga
     7321 aacggcataa gtacaattgc taagactgta aagcacagta ctgctactag gtagctttta
     7381 taaatgttgc tcattttatt ctctccttat atatttcatt gaaatgctca tcgacgaatt
     7441 tattcatctt tcttgcgtta aatctccagc gattaaaatt ctcatcaggg taatgcacaa
     7501 ttccttgcgc tcttagttct ttttcaaatc taggatgaaa tagtaatctg tccttgatag
     7561 tctcatcaga tgcaattttt aatttcttct ttaagtcact catgttccat acagggtcta
     7621 atgaataacc aattagctca tcatattcat cttttgtgat aagtacatgt gtttcaggta
     7681 ttggaactgt tacgtttaaa atatgtggca tttctatctt tcctttcgtg tataatgttg
     7741 ttatctccta gtgaaaggag gtgatattgg tgtatattga tcctttaaaa aatgttcgtt
     7801 tttctattaa taacgtaatt agtaatgttg aaatttctaa aagtatggca attaaacaat
     7861 ctttaaaacc taagtaccaa ttagatataa ttaatagaaa caacataaat ttattttctg
     7921 acttcaaagt agactttcat ctaaacaact taattgaaat gaattttaat ttgcgtaatt
     7981 ctttttcatc tctaacattt caaagaaatt tattttctga agaaacgata aaatctttta
     8041 aggaactcta taggtttgat gatgagattg tacttcaagc acaacagacc attagagatt
     8101 tttatatcaa tccaactgct atctctactt tggctgaagc catcaattcg acctatccaa
     8161 taaatgagca aagtacctac aagagacacg atgaatttgt caaacgtatc gaaaatgatt
     8221 ttccacatcc tttcaaaaag ttaataagat ggtctaatgg cattgcagca ggtgctgaca
     8281 ttcaaatctt tgtaacaaac tatataaacg agaacgattt acatattcaa aattcattga
     8341 tagttgctat agtttgttta ttaagttttt tatcgaccta ttgttcacat tctaaaaagt
     8401 aataataagg cctaatttag ttaaccttct ttaacaactc tgcaactgct cgcaacaatt
     8461 cagggttgtt acttctttct aaacagtaac tagcatgctt tagtaatttg agttttaatt
     8521 tatttttttc tttcgcgatt ctaaattttt gtaacatttg ttatgcctcc tttgcatttc
     8581 caaaaattta atctaactta aattcttttc catctattaa tccataaaag ttatttttta
     8641 aatgcggatg tctttcaagc gtcatttcaa taaaacgcgg gtctatcatt aagtcgtagc
     8701 catcgttgta ttgaatatta acgggtcgtc tattaccttc ttcgtcatag tagtaataga
     8761 tgactttttt gttttgagct tgcattgttc gttcctccta ttaagatgtt tgtttttctc
     8821 ctaaaaactt attaacaaag tattgttgtc ctttgcctgt tacttttggc gtcttactaa
     8881 ttgatgtgtg accgtccgaa tgtgtgattg atgtttcttt aatttcgaat aactcacgtt
     8941 ccattgaata ctgtgtaggc atgttataat ccacaccctt gcgtttaata aggaatccgt
     9001 tttgacgtaa ccactcaaac aatctgcgtt gcccgatgtt tataccgttt tgtttaatga
     9061 tctttgctaa ctctccaact aaaattgatg tcttagtagt agctactgca tctgcaaata
     9121 caatttttgg tttatcacgt tcaatctttg tttctaattg attgattgtg ttgttagcaa
     9181 tttttaaagc acgttgcata atcatttctg gactgttcca tgctttctct acttggatga
     9241 aatactctct aaaatcaaaa cctttttctg tacctgacat catcgcaaca tgtttagcta
     9301 catcaagtgt taaagcataa tcttctagtt gtcttacagc tccgttatta acaaccgtac
     9361 ttgtaagtac acttgtaaaa tccctatttt ctttgaaatg cttcaagtta atttctgccc
     9421 aagcgctaaa acgcttttta acttccaaag ctttatataa ctctcttgca cttattgcga
     9481 tttctccatt ttctttttct tgtatgttga acatttcgcc gatgttcgat tttgttttta
     9541 atgcttgcat attgtttatg ctcctttcgt gtataatgtt gttatcaacc taaggaggtg
     9601 ataagtatgg acataatcgc gatttgtatc gcaattttta gtttcttact gactgcactt
     9661 aaatattatt tagactatat gaaagattct cttaacatcg atgttatacc taccagaagc
     9721 tttaattact tggtcgatga caaatcaagt tacaacgata taacatttat taatttcaca
     9781 aagtttccca tttctgttat tgacgttgaa tttgatatta aaaataaagt aaatgaacaa
     9841 aaaacgttca aacctatacg atataaagat aaaaactact ccattccatt tactttagga
     9901 ccttatgaaa gtgtagaatg tacttttttg ctcgaagaat atccagtgat atgggaatgg
     9961 gatgtgacta tcaaagtcac taccaacaaa ggaatctata taaagcctgt tatcatagaa
    10021 tcgcggacag aacaccgaga atcagagcca caagtgacag agttaacatc agcaaataag
    10081 gtaagtgctc tttccaaccc caaggatggt tttttaaaga agtttttata tcatttaaaa
    10141 ccttaaacat ttaaaatcct ccctttccgt cactctttaa ttggagtggc gttgattttt
    10201 tcgtctaact ttttcaatgc taatttgtaa ataactgaag catgttcggt tttaaaatga
    10261 gattcagcaa taattttcaa tgtttctaat ttatttcttg catcaccgta tgtggtactt
    10321 tctgatagaa caccttctaa aatttgttga actcgataat ctaaaagttt taagtcttta
    10381 ttgatgcatt gttcgacaca ctcttctttg gttaacgtga tttgttccat tgtgtccctc
    10441 cttaagttca tataacatga actttttctt taaaaaaata taagtgtatt ttctctaccg
    10501 gtatatctag tagttgtata gctttccata tttcactgtc tttccaacca actttaccgt
    10561 tgagttttaa ggataaactt ctctctgaca atttcatagc aattgcaaaa ttgtactgag
    10621 tgccatactt ttctactatt ttcccgctca aacgtgagta gtcgtaacac ataaaagcac
    10681 ctcctctcaa gttcacgtat catgaactta actatacttt acaccttgtt ttgaatcaag
    10741 tcaatacaaa aattcatgat ttatgaactt ttttgttgaa tttttgttca acaagcttta
    10801 ttatgaagtt atcaaacgga ggtgcactaa atgagagaaa aagtttcaaa tagacttaaa
    10861 cacattatga aaataagaaa cttaaaacaa gtagatatca ttaataaatc gaaaccttat
    10921 caaaagaaac taggtatatc tttaagtaaa agcactttat ctcaatatat taacgacgta
    10981 caatcacccg accaagatag aatttaccta ctttctaaaa ctctgaacgt tggtgaagcg
    11041 tggcttatgg ggtatgatgt agattcttat cgagttcctg atgaagaacg tcaagatgaa
    11101 acgataatgt caaaaatcaa taacatattt tctcaactca cacctccccg ccaagaaaac
    11161 gtacttaact atgcaaatga acaattggaa gaacagaata aagtcacttc tatagatgga
    11221 tataaagagt ctaaactagt atcgtatatt gcatgtggtg caactggtgc tggcatagga
    11281 gaagaattat atgatgacat attgcatgaa gaagtatttt ttaaagaaga cgaaacgcca
    11341 tcaaatgctg atttttgtat tttagttaat ggtgattcaa tggaacctat gttaaaacaa
    11401 ggaacatacg cttttattaa gaaagaagat tctattaaag atggtacaat tgcactcgtt
    11461 gtattagatg gagtaagtct tatcaagcgt gtagatatat gcgaagacta tattaatttg
    11521 gtatctctaa atccgaagta tgatgatatc aaagtcgctt cgtttagtga tattaaagta
    11581 atgggcaaag ttgtattgtg attaataacg tatatttagc gctttaatat aaatataaac
    11641 aaaggagaaa ttgacatgaa aaaagcaatc ttaactttaa gtcttatatt tattacctac
    11701 tacctcactt ttaaatatat gtggattaaa gaattgaagt tttaacagcg cctatgtggc
    11761 gcttaaatat aaaaagtaag caaaggagaa atgaaaatga ataaaatatt tagagttctc
    11821 actgttagct tgtttttctt cacattttta ataaaaaaca atctagcata tgctgatgta
    11881 ggggtaatca atcttagaaa cttttatgct aattatcaac ctgaaaagct tcaaggagtt
    11941 agttctggaa atttttctac ttctcatcaa ttagagtata ttgatggaaa atacacttta
    12001 tattcacagt ttcataatga atatgaagcg aagagattaa aagatcataa agtagatatc
    12061 tttggaataa gttactcagg tctttgtaat acaaaatata tgtatggtgg aattacgttg
    12121 gcaaatcaaa atttagataa acctagaaat atacctatta atctctgggt caatggtaag
    12181 caaaatacta tatctacaga caaagtttct actcaaaaaa aagaagtaac tgctcaagag
    12241 attgatatta agttacgaaa atacctacaa aacgaataca atatatatgg ttttaataaa
    12301 acaaagaaag gtcaggaata tggatataag tcaaaattta attctgggtt taacaaagga
    12361 aaaattactt tccatttaaa taatgaacct tcttttacat acgatttgtt ttacaccgga
    12421 a
//
LOCUS       DBNENW010000075        11997 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNENW010000075 DBNENW010000000
VERSION     DBNENW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.075"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1314
                     /gene="xseA"
                     /locus_tag="AC1LW8_001643"
     CDS             <1..1314
                     /gene="xseA"
                     /locus_tag="AC1LW8_001643"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIP4773473.1"
                     /translation="ALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKE
                     SVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQK
                     LEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQ
                     ISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKT
                     PIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKH
                     LEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQ
                     RYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLR
                     GYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            1307..1537
                     /locus_tag="AC1LW8_001644"
     CDS             1307..1537
                     /locus_tag="AC1LW8_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIP4773474.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            1515..2396
                     /locus_tag="AC1LW8_001645"
     CDS             1515..2396
                     /locus_tag="AC1LW8_001645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP4773475.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            2828..3280
                     /gene="ahrC"
                     /locus_tag="AC1LW8_001646"
                     /gene_synonym="argR"
     CDS             2828..3280
                     /gene="ahrC"
                     /locus_tag="AC1LW8_001646"
                     /gene_synonym="argR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AhrC/ArgR"
                     /protein_id="HIP4773476.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            3296..4975
                     /gene="recN"
                     /locus_tag="AC1LW8_001647"
     CDS             3296..4975
                     /gene="recN"
                     /locus_tag="AC1LW8_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIP4773477.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            5126..6547
                     /gene="lpdA"
                     /locus_tag="AC1LW8_001648"
     CDS             5126..6547
                     /gene="lpdA"
                     /locus_tag="AC1LW8_001648"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIP4773478.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            6563..7555
                     /locus_tag="AC1LW8_001649"
     CDS             6563..7555
                     /locus_tag="AC1LW8_001649"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIP4773479.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            7555..8538
                     /locus_tag="AC1LW8_001650"
     CDS             7555..8538
                     /locus_tag="AC1LW8_001650"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIP4773480.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            8551..9825
                     /locus_tag="AC1LW8_001651"
     CDS             8551..9825
                     /locus_tag="AC1LW8_001651"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIP4773481.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            10176..10613
                     /gene="brxB"
                     /locus_tag="AC1LW8_001652"
     CDS             10176..10613
                     /gene="brxB"
                     /locus_tag="AC1LW8_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacilliredoxin BrxB"
                     /protein_id="HIP4773482.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            10627..11607
                     /locus_tag="AC1LW8_001653"
     CDS             10627..11607
                     /locus_tag="AC1LW8_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIP4773483.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(11734..11913)
                     /gene="prli42"
                     /locus_tag="AC1LW8_001654"
     CDS             complement(11734..11913)
                     /gene="prli42"
                     /locus_tag="AC1LW8_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HIP4773484.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         4480 a         1630 c         2258 g         3629 t
ORIGIN      
        1 gctttaacga aatatattaa atataaattt gatcaagatc cacatctcca atctgtattg
       61 attaaaggtg agctttcaaa tttcaaaaag cattcaagtg gtcatttata ctttaatgtg
      121 aaagataaag aaagcgttat aagtgccatg atgttcaaag gtagtgcttc taaattaaac
      181 ttcgaaccta aagaaggaga tgaagtctta ttagaagcac gtgtttctgt ttttgaacgt
      241 cgtggaaact atcaaattta tgtaaataaa atgcaattag atggtatagg gaacttatat
      301 caaaaattag aagcattaaa gaaaaaatta actgaagaag gttgttttga taaagcgaat
      361 aagaaatcaa tacctaagtt tcctaaaaaa attgcagttt taacagcgag tacaggtgcc
      421 gcaattcgag atatccattc aacgataaat agtcgttttc cattagctga acaaatacaa
      481 attagtactt tggttcaagg tgaaaaagca aaggacgaca ttattgaaaa aattgaatat
      541 gcagatagtt taggtgtaga taccattatt gtaggtcgag gcggtggatc catagaagat
      601 ttatggaatt ttaacgaaga agctgtcgtc cgtgcaatat ataattgtaa gacaccgatt
      661 atatcagcag taggtcatga aacagacttt acattaagtg attttgctgc agacatcaga
      721 gctgcgactc caactcaagc tgctgttatt gcaacaccag accaatatga attgctgcaa
      781 caaatacagc aatatcagtt tacattgact cgtttcatca agaaacattt agaacaacaa
      841 cgtaaacatg ttgaacattt gtcatcgtat tacaagttta aacaaccaac tttattgtat
      901 gatcaacaga tacaacgtcg agatgattta gaaaagagac tgaaacaaca aattcaggca
      961 acttttgaac aacagagaca tcgattaatg ctgttgcaac aacgttataa tttaaaagca
     1021 ttactaagta gtgttaatca agaacaacaa aataatcttc aattgacaaa ccaacttgtt
     1081 aaattattga attctaagat attaagttat aaaaatgatt tgaaaaataa agtagaaaac
     1141 ttaaacaatt taagtccaac taatacaatg ttgcgtggat atgcaattgt taataaaaaa
     1201 gacgaagtca ttacgagtac taaagattta acagaaaatg atcaattgac gttaacaatg
     1261 aaagatggct tagtagatgc aaaagttacg aaagtaaggt gtaataatga ctaaagaaac
     1321 gcaaagtttt gaagaaatga tgcaagaatt agagcaaatt gttcaaaaat tagataatga
     1381 aacagtatct ttagaggaat cattagattt atatcaacgt ggtatgaaac tatcagcagc
     1441 ttgtgacaca actttaaaaa atgccgaaaa aaaggtgaat gacttaataa aagaagaagc
     1501 tgaggatgta aaaaatgacg aatctaccga tgaataaatt aatagatgaa gtcaataatg
     1561 aattatcggt tgcgataaat aaatcagtaa tggatactca gctagaagaa agtatgttgt
     1621 attcattaaa tgctggaggt aaacgcatcc gaccagttct gttattactc actttagatt
     1681 cactaaatac cgagtatgag ttaggtatga agagcgcaat tgcactagaa atgattcata
     1741 catattcact tattcatgat gacctaccag cgatggataa tgatgattat cgacgaggaa
     1801 aattaacaaa tcataaagta tatggtgagt ggactgcgat attagcaggt gatgctttat
     1861 taactaaagc atttgaactt atttcaagtg atgatagatt aactgatgaa gtaaaaataa
     1921 aagttctaca acggctgtca atagcaagtg gtcatgttgg aatggtcggc ggtcaaatgt
     1981 tagatatgca aagcgaaggc caaccaattg atcttgaaac tttggaaatg atacacaaaa
     2041 caaaaacagg agcattatta acttttgcgg ttatgagtgc agcagatatc gctaatgtcg
     2101 atgatgcaac taaagaacat ttagaaagtt atagttatca tttaggtatg atgttccaga
     2161 ttaaagatga tttattagac tgctatggtg atgaagcaaa gttaggtaaa aaagtgggca
     2221 gcgatcttga aaataataaa agtacgtacg tgagtttatt agggaaagat ggcgcagaag
     2281 ataaattgac ttatcataga gacgcagcag tggatgaact aacgcaaatt gatgaacaat
     2341 tcaatacaaa acacttatta gaaatcgttg atttatttta tagcagggat cactaaaagc
     2401 aaatatatcg attagcaaca attgtaaagt ttgaaatgtc tcaaaaggca tattcaactg
     2461 acgttgtttg ttaatcgatt tttctcatgt tgaaacttaa aattatgttg aagactttca
     2521 tttaaaaaat agaattttca atttctatac gacaatattc gtatctgtga tatcaatgtg
     2581 attttaatta aatatgttca aacaattgac gcttattaaa tctaataatt agcaagttta
     2641 ttacacatat attcattttt atacatattt taagcaattg ttatgcgtaa tatgaaaaat
     2701 atttataaat agataaccaa gatagataaa ccatgtttac cctttttacg taaaggtgtt
     2761 tagtagtcgt aaatcagtgc atgtgttaaa ataaatgtat aaatattcgt aatcagaggt
     2821 gtttactgtg cccaaaaaat cggttaggca tataaaaatt agagaaatta tttcaaatga
     2881 acagatagag acacaagatg aattagttaa acgattaaac gattatgatt taaatgtcac
     2941 tcaagcaact gtttctcgtg atattaaaga actacaactt attaaagtac ctataccttc
     3001 aggtcaatat gtttatagtt taccaaatga tagaaaattc catcctttag aaaaattggg
     3061 acgttattta atggattcct ttgttaatat agatggtact gataatttac ttgttctaaa
     3121 aacgttacct ggtaatgcac aatctattgg agctatatta gaccaaatca attgggaaga
     3181 agtactaggc acaatttgtg gtgatgatac ttgtttaatt atttgtcgaa gcaaagaggc
     3241 aagtgatgaa atcaagtcaa gaattttcaa tttgttataa ggatgcgatg agtttatgtt
     3301 acaaacctta tcaatcaagc aatttgctat tattgaagag ttagaaattc aattttctga
     3361 tggtttaact gttttaagtg gtgaaacggg ttcaggtaag tcaattatta tagatgcaat
     3421 tggacaatta attggaatga gagcttcatc tgattttgta agacatggtg aaaaaaaggc
     3481 tgtcatcgaa ggtatatttg atattgacga gagtaaagat gctatacata tattgaaaaa
     3541 tatggatatt gatgtagatg aagatttttt attagttaaa cgggaaattt tcagctctgg
     3601 taaaagtctt tgtaaaatta ataatcaaac agttacttta caagatttac gaaaagttat
     3661 gcaagaatta ttagatattc atggccaaca tgaaacgcag tctttgttga aacaaaaata
     3721 ccatttaaca ttattggata attacgcaga atcacgttat caagatttgt tggataaata
     3781 tcatcaaact tttcaaaatt ataaagccaa aaagcaagag ttagaagatt tagaatcagc
     3841 agaccaagca ttgctgcagc gtttagattt aatgaaattc caattagaag aattgtctga
     3901 agcacatcta aaagaaggcg aaatagagca acttgaaata gatattaaac gcattcaaaa
     3961 ctctgaaaaa ttaagcttag cgcttaataa cgcacatatg acattaacgg atgaaaatgc
     4021 tatcactgat cgtttgtatg aactaagcaa ccatttgtta acaataaacg atatagttcc
     4081 aaacaaatat gacaaattaa aagaagatat tgatcaattt tactacattt tagaagatgc
     4141 aaaacatgag ttatatgatg aaatggctaa tacagaattc gacgaacaag tgttaaacga
     4201 gtatgaatct cgtatgaatt tgctgaataa tttaaaacgt aaatatggaa aagatatttc
     4261 agaattaatc gcatatcaag aaaagcttaa taatgaaatc aataaaattg aaaactatga
     4321 acaaagtaca tcgcagttac gagaagaaat aaatgcattg tataatcaag ttatagaggt
     4381 tggacaagcg ttatcaaagc agcgtcgtat agtcgctagg gagttaagag accacattgt
     4441 atcggaaatt caaaacttac aaatgaaaga cgcaaatctt gagatttcat ttaaaaaatt
     4501 agaagaaccg aatattgatg gaatcgaatt tgtagaattt ttaatcagtc caaataaagg
     4561 ggaaccatta aaaagtttaa ataaaattgc gtcaggtgga gaactttcta gaattatgtt
     4621 agcgttaaaa agtatttttg ttaaatcgag aggtcaaact gcaattttat tcgatgaggt
     4681 tgactcaggt gtatctggac aagctgcaca aaaaatggca gagaaaatgc gagatatagc
     4741 tgaatatata caagttattt gtatctctca cttaccacaa gttgcatcga tgagcgacca
     4801 tcatttatta atttcgaaat catcaaaaga tgatcgtaca acaacacaag ttcaagagtt
     4861 aattagcgat gataaagtag atgaaattgc acgaatgatt tcaggtgcaa gtgttactga
     4921 tttaactcga gaaaatgcaa gagaaatgat acaacacaat caaagacgta gataatttcc
     4981 aaagtgtgtt gaatactcat ttatggattt atttatgtgc attcaaacta aatagacaat
     5041 catgtaaaat aggtaattaa tggacatgaa aactatgtat taataaattt tcaatggata
     5101 tatatcatta gttaggagta agaacatgtc agagaaacaa tatgatttag tcgttctcgg
     5161 tggaggtact gcaggatatg ttgctgcgat acgtgcgtct caattaggta aaaaagtggc
     5221 aatagtagaa cgacaactat taggaggtac ttgtctccat aaaggttgta ttcctactaa
     5281 atcgctgctt aaatctgctg aagtatttca aacagtaaaa caggcagcaa tgtttggcgt
     5341 tgatgtcaaa gatgctaatg ttaattttga aaatatgttg gcacgaaaag aagacattat
     5401 taatcaaatg tatcaaggtg taaagcattt aatgcaacac aatcacattg acatttataa
     5461 tggcacggga cgtattctag gtacatctat attttcacct caaagtggca caatttctgt
     5521 tgaatatgaa gatggtgaat cagacttatt accaaaccaa tttgtattaa ttgcaactgg
     5581 ttcttcgcct gcagagttgc cattcttatc atttgatcat gataaaattt tatctagcga
     5641 tgatatatta tcattaaaga cgttaccatc aagtattggt attattggtg gtggtgttat
     5701 cggaatggaa tttgcatcat tgatgataga tttaggtgtt gacgtaacag ttatagaagc
     5761 aggtgaaaga attttaccaa ctgaaagtaa acaagcttca caactattaa aaaaatcatt
     5821 gtcagcacgt ggtgttaaat tctatgaggg aataaaactt tctgaaaatg atataaatgt
     5881 taacgaagat ggtgttacgt ttgaaatttc ctcagacata attaaagtag ataaagtgtt
     5941 gctatcgatt ggtagaaaac ctaacacatc agatataggt ttaaacaaca ctaaaataaa
     6001 actttcaaca tcaggacata ttttaacgaa cgaatttcaa caaactgaag ataaacatat
     6061 ttatgcagct ggtgattgta ttggaaaatt acaattggca catgttggat caaaagaagg
     6121 tgttgtggct gttgatcata tgtttgaggg gaacccaatc ccagtaaact ataacatgat
     6181 gcctaagtgt atttattcac aacctgaaat tgcttctatc ggtttaaaca ttgaacaagc
     6241 aaaggcagag ggaatgaaag ttaaaagttt taaagtacca tttaaagcaa ttggtaaagc
     6301 agtgattgat agccatgaca caaacgaagg gtatagcgaa atggtgattg atcaatcaac
     6361 tgaagaaatt gtgggtatta atatgattgg tccacatgta acagaattga ttaatgaggc
     6421 atcactgttg cagttcatga atggctcggc attagaatta ggactaacaa cacacgcaca
     6481 tccttccatc tctgaagtgt tgatggaatt aggattgaaa gcagaaagta gagctattca
     6541 cgtataaata ggaggattta acatgattga ttataaatca ttaggcctta gcgaagaaga
     6601 cctaaaagta atatataaat ggatggattt aggtagaaaa atagatgaaa gactgtggtt
     6661 attgaaccgt gcaggtaaaa ttccatttgt tgtaagtggt caaggacaag aagcaactca
     6721 gattgggatg gcatacgcat tagaagaagg tgatattaca gcgccttatt atagagatct
     6781 agctttcgtt acttatatgg gtatctcagc gtatgatacg tttttatcag cgtttggaaa
     6841 gaaagacgat gttaactctg gtggaaaaca aatgccatca cactttagta gtagagctaa
     6901 gaatattttg tctcaaagtt caccggtagc aactcaaata ccccatgctg ttggtgctgc
     6961 tttagcttta aaaatggatg gtaagaagaa aattgcaaca gcaactgttg gtgaaggtag
     7021 ctcaaaccaa ggtgacttcc atgaaggttt aaacttcgcg ggtgtacaca aacttccttt
     7081 tgtatgtgtc attataaata ataaatatgc aatttcagta cctgattcat tacaatacgc
     7141 agctgaaaaa ttatcggata gagcacttgg ttacggtata catggtgaac aagtagatgg
     7201 taatgatcca ttagcaatgt ataaagcgat gaaagaagca agagatcgag ctatttctgg
     7261 tcaaggttca actttgattg aagcagtaac aagtcgtatg acagctcatt catcagatga
     7321 tgacgaccaa tatcgtacga aagaagagcg tgaagcgctt aaaaaagctg actgcaatga
     7381 aaagttcaaa aaagagttgc tttcagctgg cattatcgat gatgcttggt tggcagaaat
     7441 agaagcggag cataaagaca tcatcaataa agcgacaaag gctgctgaag acgcgccata
     7501 tccaagtgtc gaagaagcct atgcatttgt gtacgaagaa gggagtctta actaatggct
     7561 aaattatctt atttagaggc gatacgccag gcacaagact tagcattaca acaaaataaa
     7621 gatgtgttta ttcttggtga agatgttggt aaaaaaggtg gcgtatttgg tactactcaa
     7681 ggactacaac aacaatatgg tgaagacaga gttatcgata cgccattagc tgagtcaaat
     7741 attgttggaa ctgccattgg tgcagctatg gtaggtaaaa gaccaattgc agaaattcaa
     7801 tttgcagatt ttattttacc tgcgacaaat caaattatta gtgaagctgc gaaaatgcgt
     7861 tatcgttcaa ataatgattg gcaatgccca ttaacgattc gtgcaccatt tggtggcggt
     7921 gttcacggag gactatatca ttcacaaagt attgaaagta tatttgcttc atcaccaggt
     7981 ttaacaattg ttattccttc cacaccgtat gatgctaaag ggcttttatt atcatctatc
     8041 gaatcaaatg atccagtatt gtactttgag cataaaaaag cttatcgttt cttaaaggaa
     8101 gaagtgccag aagaatatta cactgtacct ttaggaaaag cagatgttaa acgtgaaggt
     8161 gaagatttaa cagtattttg ttatggatta atggtcaatt attgtttaca agcagcggat
     8221 attttagctg cagacggtat caacgttgaa gtcgttgatt tacgaactgt ttatccatta
     8281 gataaagaaa caattattga tcgtgctaaa aatacaggta aagttttact tgtaactgaa
     8341 gataatttag aaggtagtat tatgtcagaa gtgtcagcga ttattgcaga gcattgcttg
     8401 ttcgatttag atgcaccaat catgcgttta gctgctccag atgtaccatc tatgccattt
     8461 tctcctgtat tagaaaatga aattatgatg aatccagaaa aaatcttaaa taaaatgcgt
     8521 gaattagcag aattctaggg agggaaagtc atggaaataa caatgcctaa gttaggtgag
     8581 agtgttcatg aaggtaccat tgaacaatgg ttagtttctg ttggtgatca tattgatgaa
     8641 tatgaaccat tatgtgaagt tattacagat aaagtgacag ctgaagtccc ttccacgata
     8701 tcaggaacaa ttacagaaat tttagttgaa gcggggcaga cagtagctat tgatacaatt
     8761 atctgtaaaa ttgaaactgc tgatgaaaag acaaatgaaa caactgaaga gatacaagca
     8821 aaagtggatg agcatactca gaaatctact aaaaaagcta gtgcaacagt ggaacagaca
     8881 tttactgcta aacaaaatca acctcgtaat aatggtcgct tttcacctgt tgtatttaaa
     8941 ctcgcttcag agcatgacat tgatttatca caagttgtag gtagtggatt tgaaggtcgt
     9001 gtaactaaga aggatataat gtcagttatt gaaaatggtg gtaccacagc tcaatctgac
     9061 aaacaagttc aaacaaaatc aacatcagta gatacatcaa gtaaccaatc atctgaagac
     9121 aatagtgaaa acagcacaat accagtaaat ggtgtgcgta aagcaattgc gcaaaatatg
     9181 gttaatagtg taacagagat tccacatgca tggatgatga ttgaagtaga tgctacaaat
     9241 cttgtgaata cgagaaatca ttataaaaac agctttaaaa ataaagaagg atataatcta
     9301 acgttctttg ctttctttgt aaaagctgta gcagatgctt taaaagcata tcctttatta
     9361 aatagtagct ggcaaggaaa tgaaattgtc ttacataaag acattaatat ttcaattgct
     9421 gttgctgatg aaaataaatt atacgtacct gtgattaagc atgcagacga aaagtcaatc
     9481 aaaggtatag ctagagaaat taatacttta gcaacgaaag cgcgtaataa gcaattgaca
     9541 gctgaagata tgcagggcgg tacatttacg gtaaataata ctggtacatt tggttcagta
     9601 tcatcaatgg gtattataaa tcatccacaa gcagcgattt tacaagtaga atcaatcgtt
     9661 aaaaagccag tagtaattaa tgatatgatt gcaattcgta acatggttaa tttatgtatt
     9721 tcaattgatc atcgtatttt agatggttta caaacaggta aatttatgaa tcatattaaa
     9781 cagcgtatcg aacagtatac tttagaaaat acaaatatat attagtgata acatagatgc
     9841 atctatcgac aacttgtttt atcttgttct tgtcgatgga tgtattattt tttggcacta
     9901 aaatatgtgc aatatattca aaaaagataa agaacaataa tcaacatggt tgaatgcatt
     9961 tttgcagtaa agtcaaaata agacatcata cttgaaacat attaatgaaa acatgtgaac
    10021 aaattagtta ccatgatttt aagcacaata atgtttggta tattgttaaa attgtgtcta
    10081 aatataggtg tgattcagat tagtttattg aacaatatgt tattaattag tagaatgagg
    10141 atagtttaaa tataaaggga taggtgattg aacttatgga catgaatttc gatttataca
    10201 tgaacggtgt tgtagaacaa gcaaggaatg aaattgaatc tgcgggatat gagcaattaa
    10261 ctactgcaga agatgttgac aaagttctta aacaagatgg tacaacacta gttatgatca
    10321 attctgtatg tggttgtgca ggtggtatcg caagaccagc agcatcacat gctttacatt
    10381 atgacgtatt acctgatcgt ctagtgacag tatttgctgg acaagataaa gaagcgacac
    10441 aaagagcgcg tgaatacttc gaaggttatg cgccttcaag tccgtcattt gcattagtaa
    10501 aagatggaaa gattacagaa atgattgaaa gacatcaaat cgaaggtcat gatgtgatga
    10561 acgtaattaa tcaattacaa acattattca ataaatattg tgaagaaaga taagaggcgc
    10621 taacccatgt taaagttaaa tccttacaag attggattta gaacaataaa aacagcagtg
    10681 ggtatgactt taggtgtaat tattagtaag ctgttaggtt tagataatta tgcttcaagc
    10741 gccatattag tcgtattatg tattaaacat acaaaagtac attcgctaca agcgattatt
    10801 tcaagattag tatcatgttt tttagtattg tttttaggtt cagcaatatt tagtttatta
    10861 ggtcagagtc caattgtact cggtattatc gtattgttat ttataccatt aactgtcgta
    10921 ttaaaagtac aagaaggtgt cattacgagt tgcgttatat tacttcatgt ttttaatgca
    10981 aaatcaattg atgcacattt aattgttaat gaaacattat tactgttaat tggactgagc
    11041 attgcattta caatgaattt aatgatgcca agtttagaca aacaactaga cgaatacaaa
    11101 tgtaaaattg agcaacaaat tgctgatatt tttagtaaat atagttatat ttgtgaaaag
    11161 tatgaagata ccattgcgat tgaatttgaa gtgttacttt taaatattaa aaaggcgaag
    11221 tctatcgcgt tccgagatgt taaaaatcat tttgttagaa acgaaaattc atactatcat
    11281 tattttgata tgcgagaaga gcaagtggaa ttgttaatga gaatgaaacc gctcatcgaa
    11341 agtatctgtc ataaagatcc acttttaaat gatgttgcgt cactgctgaa agaaattggt
    11401 ctaaatgtga atagtaatga ctatacagca atgcgtttac acaatttgta tgagacgcgt
    11461 ttgaaattgg atgaacttcc attgccaaca acacacaaag cgcttaactc gagagcaagt
    11521 attattcaaa tattaaatga attagaagaa tatttacaaa tcaaatcgca atttggttca
    11581 ttgaaaatgc atagtgaagt agcttaaaaa ataatccatc cgtatgaccc atgtttgaaa
    11641 ataatcgttg caatacgata acttattaat acatgcgttt ataacggatg gatttttaat
    11701 tgtatgattt tgtataggta gttgagatat agtttataaa attggaatga tactcgttaa
    11761 taacaatgct aatacaactg atacaagcat aacgataatg ataattctta aaactttatt
    11821 ttgcattttg tgtgcctcca aatgaataac ctttgtatta ataattacga cataagatat
    11881 aaaactttta acttgagacg atataactag caatcatcat taaggaatat aataaaaaac
    11941 atgagtaagt ttgcttatgt cattttcctc attatacgat atctttgaaa aatcaat
//
LOCUS       DBNENW010000076        11952 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNENW010000076 DBNENW010000000
VERSION     DBNENW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.076"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(143..763)
                     /locus_tag="AC1LW8_001655"
     CDS             complement(143..763)
                     /locus_tag="AC1LW8_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIP4773485.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPKTPEETDNQL"
     gene            1162..1770
                     /locus_tag="AC1LW8_001656"
     CDS             1162..1770
                     /locus_tag="AC1LW8_001656"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIP4773486.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            2107..3315
                     /gene="gltS"
                     /locus_tag="AC1LW8_001657"
     CDS             2107..3315
                     /gene="gltS"
                     /locus_tag="AC1LW8_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIP4773487.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKLLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTSQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(3363..4412)
                     /gene="fni"
                     /locus_tag="AC1LW8_001658"
     CDS             complement(3363..4412)
                     /gene="fni"
                     /locus_tag="AC1LW8_001658"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIP4773488.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMYSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(4443..5390)
                     /gene="corA"
                     /locus_tag="AC1LW8_001659"
     CDS             complement(4443..5390)
                     /gene="corA"
                     /locus_tag="AC1LW8_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIP4773489.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            5487..5978
                     /locus_tag="AC1LW8_001660"
     CDS             5487..5978
                     /locus_tag="AC1LW8_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIP4773490.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            5975..6394
                     /locus_tag="AC1LW8_001661"
     CDS             5975..6394
                     /locus_tag="AC1LW8_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773491.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(6502..7422)
                     /locus_tag="AC1LW8_001662"
     CDS             complement(6502..7422)
                     /locus_tag="AC1LW8_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIP4773492.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGPQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            7630..8268
                     /locus_tag="AC1LW8_001663"
     CDS             7630..8268
                     /locus_tag="AC1LW8_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773493.1"
                     /translation="MTNPNQRLEPFDETFQQSNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(8514..10445)
                     /locus_tag="AC1LW8_001664"
     CDS             complement(8514..10445)
                     /locus_tag="AC1LW8_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIP4773494.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(10458..11105)
                     /locus_tag="AC1LW8_001665"
     CDS             complement(10458..11105)
                     /locus_tag="AC1LW8_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIP4773495.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            11226..11777
                     /locus_tag="AC1LW8_001666"
     CDS             11226..11777
                     /locus_tag="AC1LW8_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP4773496.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
BASE COUNT         3958 a         2074 c         1756 g         4164 t
ORIGIN      
        1 aaaatttctg aaattgaaat atttaaatag tatcttcttt gcatgttaag tatcattagc
       61 gctagatacg catttcccat atctttattg ccttatttgt aaaaaaatat cccagcatca
      121 ttgtgccgag atatttatat cattacaatt gattatctgt ttcttccggt gttttcggtg
      181 tttcagatgt cactggttga tttttaattg tagacgggta ttttgggctt tcgccatatt
      241 tatttgccgg cttactgtca aagcatagga atactatcat aacaattgtt aggcctaaat
      301 aaacaaaata aagaagtact aacaaaatat taagacccat cggcattaat gtaaaatcac
      361 tgtcataata actatcgata atctgtaata ctatataaaa tataatactg aatactgtca
      421 taatcattgg aaataacatt gttcttgata tatcgtgaaa tcttcgaacg cacaacgcta
      481 aatttggaat aaacgttgcc aaactataga caaaagtata cacagatgta aggataatca
      541 tcaatatact cataactatt aatgtttcgt tatctgccgc tatagaaata aagaatagaa
      601 ataggtttat tattagcaca cacacagctg gaaccataag tatcaaatgc catagtgcca
      661 tataccaata ttcactacgt cttgatctcc ccttaaaatt tacataattt ttccaaaata
      721 atacgaatga tttcataaaa cctacttgag gtaattgttc cattgtaatc tccctttcgt
      781 taatcatatt tatattttta attattgtta ccgttataat ttacaagatt cattatcaag
      841 ccacttcatt tcaatcgtca atcactttgg aaaatgaact ttatatttca tcactagtaa
      901 aatgaaaacc cgctacaagt acacatctat atggagactc atttgaaagt caacgcttcg
      961 ttaactatac taaaaatatg tcacactgca atgttcacgt ttaaaagagt ctcaatctct
     1021 gcaaataaaa tattccataa caaagtatat actttacatt tttataattc ttaacaatac
     1081 tattttatca aacatttacc acaataaaaa tatctttatc atttttattt aatttaatca
     1141 tataattgcg aggagaatat tatggatttc gttaataatg atacaagaca aattgctaaa
     1201 aacttattag gtgtcaaagt gatttatcag gataccactc aaacgtatac aggctacatc
     1261 gtggaaacgg aagcttactt aggtttgaat gatcgtgcgg ctcatggcta tggcggtaaa
     1321 ataacaccta aagtcacgtc attatataaa cgtggtggta caatttatgc acatgtcatg
     1381 catacgcatt tactcattaa ttttgtaaca aaatctgaag gtatacctga aggcgtactt
     1441 atacgcgcaa ttgaaccaga agaaggttta tccgctatgt tccgtaacag aggtaagaaa
     1501 ggctacgagg taacgaatgg cccaggaaaa tggactaagg catttaacat tccacgggct
     1561 atcgatggcg ctacgttaaa tgactgtaga ttgtctattg atactaagaa tcgtaaatat
     1621 cctaaagata ttattgctag tccacgaatc ggtattccaa ataaaggtga ttggacacat
     1681 aaatctttac gttacacagt gaaaggtaat ccatttgtgt ctcgcatgcg taaatcagat
     1741 tgtatgtttc ccgaagatac ttggaaataa atgccatctt tcattgatta ctatcatgaa
     1801 aatgaaatct atctccttat aagtcaatca atcgtgccgt caacatgcgg atgggttgat
     1861 tgtttttctt tgtatccatc atattttttg attcatctcc tcttattgaa cttgttctta
     1921 attataaaat ataacaatag aattatttat aattattaaa tttagatgca ttaatattat
     1981 tgatattatt ttcaaaaact agaaatattg atttgttgca tgtataatgt taaaagcgcc
     2041 cttttataac gcttacatat aaaagcttat ttagggagag ggatattcaa caagggggat
     2101 ttgaaaatga tagaacttaa tgcaattaca acattatgtt tagcttgtat cctttattta
     2161 cttggtaagg ctatcgttaa tcacgttaat tttttaaaac gtatttgtat accagcacca
     2221 gtgattggcg gcttaatctt tgctatttta gttgcggctt tggattcatt tggcatggtt
     2281 aagattaaat tagatgcttc attcattcaa gatttcttca tgttagcatt ctttacgaca
     2341 atcggtcttg gtgcatcatt gaaattattt aaattaggtg gcaaactctt gctattatac
     2401 tttatgtttt gtgctatcat ttcagtcatt caaaacatag ttggtgtatc actagcaaaa
     2461 gtattaaata ttaaaccttt gttaggatta acagcaggtt ccatgtctat ggaaggcggt
     2521 catggtaatg ctgctgctta tggtaagaca attcaagatt taggtattga ttcggcactg
     2581 acagcggctc ttgcagctgc aactttaggt cttgtatttg gagggcttat cggtggtcca
     2641 gttgtaaaat ttttaatcaa acgttataac ttaaagcctc aacatagtga cgatacattt
     2701 aaagattata gccaagtagc atataacgaa catttacata gtaaatttaa tgccactgaa
     2761 gtattcttca ttcaatttac aatcgttgta ttctgtatgg cagttggaag ttatttcagt
     2821 catttgttta catctcaaac agggattaat gttccaattt acgttggctc attatttgta
     2881 gctgttattg tccgaaatat ctctgaaagt tttaatttta atattgtaga tttaaaaatt
     2941 actaatcaaa ttggcgatgt cgcattaggt attttcttat ctcttgcgct aatgagcatt
     3001 caattaatcg aaatttataa acttgctata cctcttatta ttattgtttt agttcaagtt
     3061 gtcgttatga ttttatttgc tgttttaatt ttatttagag gtttaggaaa agattatgat
     3121 gctgcagtaa tggtaggtgg ttttatcggg catgggcttg gtgcaacgcc aaatgccatg
     3181 gcaaatttag atgttattac taaaaaatat ggtaactcac ctaaagcata tttagttgta
     3241 cctattgttg gtgcattctt aatcgattta attggtgtta tagtcattat gggattcatt
     3301 caatggttta gttaaacatc aaactcataa ataaaagagg aggtcttcgc ctcctctttt
     3361 atttatcctc gatgtatatt caagctacgt tgttctatcc atgacaatat ttccggacta
     3421 aatacgattt gtttttgtgt taagtcgtca atatttttag catctaacat cgtcattatt
     3481 gatttcatgt gttcaataaa tgattctaca taagctactg tatgtgcaat gccattattt
     3541 tcaacttgat ttaaaaacgg acgagacata ccagttgcct ttgcaccaag tgctaaactt
     3601 ttaattgcat cgagtggtgt acgtaaacca ccactcgcga aaactgaaat ttcgctttga
     3661 taagccgttg tttcaagtaa tgactcaact gtagactgtc cccatgatga taagtaatcc
     3721 atatctttat ttgcacgacg ttcattttca atatctacaa agttagtacc acctttgcca
     3781 ctaacatcga catacttgac gcctatttgt tgtaagtcat gcattaattc tttgctcata
     3841 ccaaatccaa cttcttttat aatgactgga acagacactc gtgatacaat cgacgctata
     3901 ttatctaacc aagtcacaaa ttcacgattc ccttcaggca taactaattc ttgaggagaa
     3961 ttaacatgga tttgtaacgc ttgtgcctca agtaattcaa ctgcttccaa agccttttct
     4021 actggtacgt ccgcaccaac attgctaaaa atcatgcctt caggattcat ttttcgcgca
     4081 atcgtaaacg tctcagccat gcgtggattt ctcaatgccg catgtgttga tccaactgcc
     4141 atcgctaagc cagtttctct tgcaactaca gctagctttt cattgatgtt tttcgtccac
     4201 tcgctaccac ccgtcattgc attaatataa accggatatg ccatcgttaa gtcaggcgtc
     4261 tgtgatgtca aatcgatatc atttacatta attgatggga tagaatgatg cacaaaacgc
     4321 atcttatcaa aatctgaata cattgcgtca gattgagcca ttgctatttc aacatgttca
     4381 ttttttctct gttctctttg aaaatcactc atgattaaac ctaccttttc gtcatttcta
     4441 atttaaaacc attttttacg tctgaaaaag atgattaata cgacagctat aacaaacatt
     4501 acagctaagc aaatataata tccataatgt aaatgtaatg ccggcatatt tacaaagttc
     4561 ataccataaa tcccagctat gaatgttaac ggtgaaaata taactgatac taacgttaat
     4621 acttgcataa tactattcat tctaaatgac gtgtatgact caaaattttc tcgtatttcg
     4681 tttgtcattt cttgagcagt acgaatgata ttacgttgct taatcaagtg gtcatcgata
     4741 tgttgaatgt atagcgaatg tttattatct ataatcaaat caccattttg tttcattgta
     4801 tcaattagct cttgcatagg aaacagtaca cgttttactt taatcaaatc cgaacgtaac
     4861 ttaaagacac tatccatgac cattttatta aagcgatcat ctacatggcg gtcttcaaaa
     4921 tgataaacac tatcttcaag tgcatataca aagttgaaat atttatcaac catcatatct
     4981 aaaattaata tgacgacatc tgcacaatct aattctgcat ctaatgtatt catatactta
     5041 tagactactt tatttaatga ttccaacgtt tgatgatgat atgttactaa tacattgtct
     5101 tgtataaaaa tatttagtgc tattggtgaa tagtttgacc ccataatact atggaatact
     5161 aagtattgat aatctttata agatttatat ttagctcgtg gcataccgtt aattgcatca
     5221 tccacttcta aatcattaaa attaaaatgt gctttaaacc attcattttc ttgttcattc
     5281 ggttcatcaa aatcatacca aacaatagtc gcatcttttg gtatctcttt gatatcatca
     5341 actactttaa acggttcata tgtagtttga taccgtatct ttaaagccat cgatactccc
     5401 cctaaataac gaattctcta ttattttatc atgaattaaa taacgtgtat gtcttaattt
     5461 attttagtat gatagtcact aaggagatgg ttattatcaa acaacttttt acacatactc
     5521 aaaccgtaac atctgaattc attgaccata acaatcatat gcatgatgca aattataata
     5581 tcatttttag tgacgtcgtg aatcgtttta attacagcca cggtctttct ttaaaagaac
     5641 gcgaaaattt agcatatacg ctatttacac tagaagaaca tacgacatac ctctcagaat
     5701 tgtctcttgg cgatgtattt actgttactt tatatattta tgattacgat tataagcggt
     5761 tgcatttatt tttaacatta actaaagaag atggtacact agcatcaaca aatgaagtaa
     5821 tgatgatggg aattaatcag cacacacgtc gttctgatgc ttttcctgaa tcattttcaa
     5881 cacaaatagc acactattat aaaaatcaac caactatcac ttggcctgaa caattaggac
     5941 ataaaatagc aattccacac aaaggagcat taaaatgaca gatgcattac aacaaaagat
     6001 tcatatcgaa ttactagatt tattagatga tgttaagttt gaattaacag aattaaatgc
     6061 acaaaaaggg ttatacatta acggaccagc aaatcagcta cttaagcgtg gcgtgcatat
     6121 ggcttatgtt caaggacaaa agcaagccat cgataatatt atgactattg tggaacaaca
     6181 gcttgaagat caacatttcc tagaacatta tgataaattt caaaatgagg ttgctcatcg
     6241 caattatgat aaaacagcta actttgcaga acttagtgat attccacgtc aattcgataa
     6301 ttttttagat caattttatc aaattaaagg gcaatacttt atcatcacac atatcaatac
     6361 acttattggt gattttcact cagaagctca ttaacaatta gtctatataa tccttgctat
     6421 attttcaaaa acaaaaccca attacgattt catgtcaaat atcatcttgc atgaaatcgt
     6481 aactgggtca tttatatgtt attagttatt ttgtgttaca tcctcatcta tcgatttggc
     6541 aatttgttta atagctttat gtgattgtct aattggataa attggaaaat catgtaccat
     6601 cttaggataa tcataaaact caatgtattg atgatgttgc aacatcattt gttcaaatag
     6661 cttcatatca ggatgtgtca tttcacgtcc accaccaaac atataaactg gtggcaatcc
     6721 ttctattgtg ccattaattg gcgatatgcg cttatctgtt aatggtaggc cattcgccca
     6781 ttttttcata atctcattga caccaaactg acttagaacc gcatcttgtt cgattaaggc
     6841 gtccgaaata tctttattag atagtgttgc atctaaaatt ggtgagatta aatacaattt
     6901 attcggtaat ggctgttgat tatctaaaag agattgtaca aaggataatg ccagtgcacc
     6961 acctgaacca tcacccatga ctacgacatt ttgaggtcct acttcagata ctaattgatc
     7021 ataaacacgt tgtatcgctt ggaaagtatc gtcaatatga aactctggtg tctttggata
     7081 gataggcagt acaacctcat ataatgtact taaagtgatt ttatccaaca atctccaatg
     7141 gaacggtgat ggttgtagtg cattgaatcc accgtgaata tataaaattt tcttatcaat
     7201 ttgatgtctg aaattaaagc gaaagacttg catatcatct aatgataatt tttctaaatt
     7261 tgctttaaca tttaatgttg aaggctgctt atgttttttt ctattttcaa tttctctttt
     7321 ataaaaaaat ctttcaacat cttgatcatt tttaaacata atcgagcgat tgtgaagcaa
     7381 atatttattg acaacgctat tcataacacg gtttctaatc aatgtcttaa cctaccttta
     7441 tatattttat gtatccaatg attgtctatc ccctacattc tttgccaaaa aaagtatata
     7501 atgtagaaga tattttcttt ttcactttca aatttaagac tacaattgaa cagtgatttt
     7561 tcatcattat aacagacaac tagacatatt gataagtaaa gaaaagaact ttatacggag
     7621 gtaccttgca tgacaaatcc aaatcaacga ttagaaccat ttgatgagac atttcaacaa
     7681 tcgaatattc atcgtggtaa gcgatatggt aagaaaaaac gttcattggt aagcatgatt
     7741 attcaaatca ttgttgttat attaaccgca atcgctggat acagcatgtg gaaacaaccc
     7801 atttttaata ttgtcttcac caaagaatct gtagattttg atggcattaa aaattttaac
     7861 gagacaattg gaaaaatgca tcaattaaat attaatttgg gtaatatcaa tgatttacaa
     7921 caaggaatcg atcgcttaat tttagtcttt aacggtttct ttattttatg tatcgttagc
     7981 ctagtcataa caatactgac aattgtattt aatcgcacag ttctaaaagt agtaaatatt
     8041 ttagtgttaa caattatgtt agtcattaca ttgtatttca gttatattat taaagcactc
     8101 gcacaagaaa ttgcagatgc tttaaaaaaa tattatttaa atgtaccacc agatcaagtg
     8161 attacagaag ctgacgctat tcataacggc atcattttac tgggctgtag catcggttta
     8221 cttgttataa gcttattctt ccgtaatcgc atgccccgtt taaaataatg ttatatagta
     8281 gggattggga cagaaatgtt attttcacaa aattcatttc gttgacccac ttcctgcttt
     8341 ttctttatta atgacctact acgtcaatgc caaattttaa tataattcct ctaacattct
     8401 acaaatcaac aaataaaaaa agtatcgata accatatttg gcaccgatac tttaaaaatc
     8461 atgtccccac aacataattt ttgattagtc acacttcaat taatttattt tatttattca
     8521 tgattgatac tattatctgc atctaacttt tgagctgtag cttttgcttt tttattactt
     8581 tgtaaaaata aacataaaat taaggcgatg atgctaaata tcgttgcaac tataaatgca
     8641 tcattaatac cttcaaccgt tgctagttta tttacaaatt gtagtaacac tttcattgcg
     8701 ccttcttgtc cgccatattg tgatgctaat tcacgcatat gatcttgtac aacaggattc
     8761 gttttatcta actcttcccc aaaagctgat aagtgttgtg tagtttgtgt tgtcattaca
     8821 gtaactaaga ttgctgtacc tatagagcct gctaattgac gcatcgtatt taagaaagca
     8881 ttaccatgag aggcaagtcg tcccggtaac gcattaatag ctgcagttac cattggcatc
     8941 attataaatg ccataccaaa tgaacgaagt acatagatac ccatgattgt catatatggt
     9001 gtatccatat ttaatttagt taattcccat gttgcataag tcatgacagc aataccaaaa
     9061 atggctaaag gttttaaacc aatcgtgtct aacaacttac cagcaaatgg tcctagtaga
     9121 cccataatta gagaaccagg taataataac aatccggaat ctaatgctga gaaaccacgt
     9181 aaattttgta agtaaattgg taataaaatc ataccaccat acaaacttaa cattacaacc
     9241 atattaataa ttgttgttaa tgtaaatgtt gggaatttca atacttctaa attcaacatt
     9301 ggcgccttca ttcttaattc tcttattaca aatagaataa taaagataat accaatcgca
     9361 aacattgttt ctatctcaac agaaccccag cctttgttcc ccgcttctga aaagccgtat
     9421 aacaaagcac caaaaccaat cgtactaaaa atgatacctg ggatatcagc tttagggttt
     9481 gttgtatatt gatataactt aaaccataca aaaccaacta aaatagcgat aatcccgata
     9541 atgaacatac cgtaaaacat cacattccaa tggtaatttt gtacaatata acctgataat
     9601 gttggaccaa ttgcaggtgc taaaatcatt gcgataccca ttgtacccat ggcagcacca
     9661 cgtttttcag gtggataaat tgtaataata acaattgaac ctaatggcat tagtacacct
     9721 gcaccaatgg cttgtaatac acgtccaacc atcatgattg ggaaattcat tgaaatcgca
     9781 cagattaatg aaccaattgt aaagagtact aacgcaacta aaaataattt tcgatatgaa
     9841 tatttattaa atagatacgc cgtaattggt attaaaatac cgtttactaa catgaatccc
     9901 gtcatcaacc attgccctgt tgacgcagaa atattaaatt ccgtattaat ttttggcaaa
     9961 gcaacattta ataatgtttg gtttaaaatc gcaataaaca taccgaataa taatgccgct
    10021 aatattttac cgcgtgaaac accttcacca aaaataaagt ttttatgttc tttttttatt
    10081 ttttcattca ctttatattc ttctgattca ggatttttag cagcaactgc ttcctcacca
    10141 ttattattag tgaatgcttc ttgatctttc tcagacccct ttgtgtaacc atttagacta
    10201 acttggctat gatcatcttg attgtcaaca cgcaactctt catgcgtcat acgttgtgtc
    10261 gattgatcag ttgttttgtc taaatcacta gctttaaatt tagattgatt tgattgacgt
    10321 gtcgtaaatt gttgttcctt ttgttggcgt ttgccttttt ttcttgatct tattaaaaat
    10381 aaattgataa ccccaacaat aatgagcgct aaaataatgt agctaataat gaaggtcgta
    10441 gtcatttaat gaccccctta atttttatgg atttttactt cagcgttcat tccaggaaca
    10501 acttgtttag acggttctga ttctagagtg attttaacag gtattacttg agaaacttta
    10561 gtgtagttac catcactatt tgatgatggc attaatgaaa agcttgcagc agttgctttt
    10621 ccaatactat caactttacc tttaatagaa gctttttgac cgtcaatagt cacatcaaca
    10681 tctttaccta cttcaacatc tttaatatct ttttcgtcaa tatttgctgt tacatataaa
    10741 tcatctaaat tgtatgcata agcgattggg ttaccagctt gcaccattga accttccata
    10801 ccatctaatt tggcaattgt acctttttga ggcattttaa gatccatatc tttcgtttcg
    10861 ccatcttgac cttgtacagt aacagttgct actttgtcac ctttatcgag tttgtcacct
    10921 tgtttaacat taagtgattt aatttgtcca gatgcaggac ttgctatttt aatttgatcg
    10981 ccatttactt ttgcattatc agttgttaca tagcttgttg ttttattcca gaaataaaag
    11041 ccagcaatcc caattgctaa cagtacaaca accgtaatga cattaattaa tatcatcttt
    11101 ttcatgaaac atttcctcct atttgctttt aaaattcact ttatttatta tagagacatg
    11161 ataactaaaa aaacaccaac aatttttata agagtgttgg ttaaaattca caaaggtgta
    11221 acaatatgaa acgacaagcc aaaatagaaa ttcaaaacgc acttgttgat ttgatggctg
    11281 aatatccttt tcaagaaata tccactaaaa tgatttgcgc gtattgtaat attaatcgtt
    11341 ctacttttta tgattattac aaagataaat ttgatttatt ggataccatc aactcaaagc
    11401 ataaagaaaa attccaattt cttctaagcg cgctacatca taatttcgaa aatattaaac
    11461 aggataagct taaactttat aaattcttca ttattattgc taagtatatc aagcacaatg
    11521 agcaattttt caaagacatt ttagttacct atccaatgaa aacattattc atagactata
    11581 taaatttagc gagagattac tatcaacaaa ttatgaatga ctatagtact acagtaacca
    11641 ataaacagct ttatgttaca tatatcatag gcggtcaagc tggggtgttt attaattggc
    11701 tttcgaatgg ttgtaatgaa tcacctgaag aagttgctga cattttattg gctaatacga
    11761 ttaaacttca aaactaataa tacggtttca ttctaataaa gaaatgctat aaaacaacta
    11821 gataaagtta aaccaaatgg atagataata gtacatatgt tacggtgcta tcaatataaa
    11881 aatgccgaga tttccacata ataatctcgg catttttatt tttgatgcta aattcatagg
    11941 tctatgaata gc
//
LOCUS       DBNENW010000077        11790 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNENW010000077 DBNENW010000000
VERSION     DBNENW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.077"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..459
                     /gene="cap8J"
                     /locus_tag="AC1LW8_001667"
     CDS             <1..459
                     /gene="cap8J"
                     /locus_tag="AC1LW8_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HIP4773497.1"
                     /translation="ENKVAGWLWAWKAVPFKLLGINTSLPFPADITVRMHNPNNIVFD
                     KNDIHIFQSPGTYFNNFSAVIYIGRGVYIAPNVGIITANHNIKNLKSHAPGEDVKIGN
                     YSWIGMNSVILPGVELGEHTIVGAGSVVTKSFPEGNVVIGGNPAKIIKKI"
     gene            468..1706
                     /gene="cap8K"
                     /locus_tag="AC1LW8_001668"
     CDS             468..1706
                     /gene="cap8K"
                     /locus_tag="AC1LW8_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HIP4773498.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISACL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEH
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGDSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTIVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            1740..2945
                     /gene="cap8L"
                     /locus_tag="AC1LW8_001669"
     CDS             1740..2945
                     /gene="cap8L"
                     /locus_tag="AC1LW8_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HIP4773499.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGICIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            2956..3513
                     /gene="cap8M"
                     /locus_tag="AC1LW8_001670"
     CDS             2956..3513
                     /gene="cap8M"
                     /locus_tag="AC1LW8_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HIP4773500.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKVNVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            3513..4400
                     /gene="capN"
                     /locus_tag="AC1LW8_001671"
     CDS             3513..4400
                     /gene="capN"
                     /locus_tag="AC1LW8_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HIP4773501.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKSEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            4454..5716
                     /gene="cap8O"
                     /locus_tag="AC1LW8_001672"
     CDS             4454..5716
                     /gene="cap8O"
                     /locus_tag="AC1LW8_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HIP4773502.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEIYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLKKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            5793..6938
                     /gene="cap8P"
                     /locus_tag="AC1LW8_001673"
     CDS             5793..6938
                     /gene="cap8P"
                     /locus_tag="AC1LW8_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HIP4773503.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(7003..7329)
                     /gene="isdI"
                     /locus_tag="AC1LW8_001674"
     CDS             complement(7003..7329)
                     /gene="isdI"
                     /locus_tag="AC1LW8_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HIP4773504.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(7336..7719)
                     /locus_tag="AC1LW8_001675"
     CDS             complement(7336..7719)
                     /locus_tag="AC1LW8_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIP4773505.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            8146..9633
                     /locus_tag="AC1LW8_001676"
     CDS             8146..9633
                     /locus_tag="AC1LW8_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIP4773506.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            10279..11238
                     /locus_tag="AC1LW8_001677"
     CDS             10279..11238
                     /locus_tag="AC1LW8_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIP4773507.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(11591..>11790)
                     /locus_tag="AC1LW8_001678"
     CDS             complement(11591..>11790)
                     /locus_tag="AC1LW8_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773508.1"
                     /translation="KESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILKPK
                     DVVLDVDSVKEIDENHLNDAT"
BASE COUNT         4085 a         1705 c         2191 g         3809 t
ORIGIN      
        1 gaaaataaag tggctggatg gttatgggcg tggaaagctg ttccgttcaa gttgttagga
       61 ataaatacaa gtttgccatt tcctgcagat ataactgtta gaatgcataa ccctaataac
      121 attgtttttg ataaaaatga tattcatatt tttcaatcgc ccgggacgta ttttaataat
      181 ttttcagcag ttatatatat aggtagaggt gtttatatag cgcctaacgt aggtattatt
      241 acagctaatc ataatattaa aaatttgaag tcacatgcac caggtgaaga tgtcaaaata
      301 gggaattata gttggattgg aatgaactca gttatattac caggagtaga attgggggaa
      361 catacaattg taggggctgg gtcggttgta acaaaaagtt ttccagaagg taacgttgtt
      421 ataggtggca atccagcgaa aatcatcaag aaaatctgag gttaattatg agattaaata
      481 aatttattgg cgattcgttt ttaatgattt taagcagtgg catcgctcaa gtcatattaa
      541 tcatcactac cccaattatt acaagactat attcacctac agaatttggt gagtttacaa
      601 ttttttcaaa tatcgcaatg attttaatac caataataaa tgcaagatac gatttgttga
      661 ttgtgaatac caaaaatgac cgtagtgcta atatactttc acaaatcagt tttttgatat
      721 cattgcttat tttattaata ctgataccaa tatttgcgat tagtgcatgt ttatacccaa
      781 actttatatt agattttatt ttcattatta ttatgttgtt tttggtaagt ttaacaaaca
      841 tttttacaaa ttatctaaat aaggaaagaa agtataaagt gttaagtttg attaatgtgt
      901 ttagagctgg atcaatggct ttacttcaaa tcattttcgg acttttagca ttaggaagtt
      961 taggattaat tattggtttt tcattatcct atatcgcagg cattacacta ggatataaaa
     1021 cgtttaaaaa gcactttaat attgtgagag ataaagaaga aactaaagca ttatttttag
     1081 aaaataaaaa tcagttagtt tattcaacac catcaatatt attaaatagt ttgtctttct
     1141 cggttgttgt gttctttata ggtattttgt ataccaatac agaagtgggt atttatggta
     1201 tggccataag agtactaggc ataccagtga caattatttc attagggtta tcaaaaatat
     1261 ttatgcaaca agccaatgac tattatattg aacatggtaa cttccgaaat ttattactta
     1321 aatttagttc catactggtt atagtttcta taattcttta tgtgccactt tatttgttca
     1381 gtgaagaatt agtcaatata ttattaggag atagctgggt tgacgcaatt acagttataa
     1441 aaattgttat cccattattt gttataaggc tgattgtatc aacggtatca ctttctgtga
     1501 ttgtattaca aaaacaacag ttagaattaa tactacaagc gttattttta ataggtacta
     1561 ctgcaacatt tgttatatca aaaatgctta atttaacttt tttaaacttt gtatctatta
     1621 atacaattgt tttaatcgta tcgtacatga tatttttcat agcactctat tattttgcta
     1681 aaaataaaca gttcaaaaat tcttagtcta aattcattaa taaaaaggac gtcgattaga
     1741 tgagtgaaaa aaagattttg attttatgtc agtattttta tccggaatat gtatcttctg
     1801 cgacgttacc aactcaattg gcggaagatt taattgcgaa tcacattaat gtcgatgtca
     1861 tgtgtggatg gccatatgaa tatagtaatc ataaacaggt ttctaaaacc gagatgcatc
     1921 gtggtatttg cattcgacgt ctcaagtatt cgaggtttaa taacaaaagt aaggttggaa
     1981 ggatcatcaa tttctttagt ttattttcaa aattcgtgat taatatacct aaaatgttga
     2041 aatatgatca gattcttgtt tactctaatc caccaatctt gccattaata ccagacgttt
     2101 tacacagact gcttaagaaa aaatattctt ttgtggtgta tgatatagca cctgataatg
     2161 cgattaagac aggtgcaact cgtccaggta gcatgattga taaactgatg cgttacatta
     2221 atagacatgt ctacaagaat gctgaaaatg tcattgtcct tggtacggaa atgaaaaact
     2281 acttactaaa tcatcaaatt tctaaaaatg ctgacaatat ccatgtgatt cctaactggt
     2341 atgacatgcg tcaattacaa gacaatcgta tctataatga cacatttaaa gcttaccgtg
     2401 agcaatacga caaaatttta ttgtatagcg gtaatatggg gcagttacag gatatggaga
     2461 cacttatctc atttttaaaa ttaaataagg atcagcctca aacgttaaca atactttgtg
     2521 gtcatggtaa gaaatttgca gatgtcaaaa cggcaataga agaccatcgt attgaaaatg
     2581 ttaaaatgtt tgagttttta acaggtacag actatgctga cgtattaaaa attgcggatg
     2641 tatgtattgc atcgctgatt aaagaaggcg tcggtttagg cgtgccgagc aagaattatg
     2701 gctatcttgc agctaagaaa gcgttggtac tcatcatgga taagcaatct gatatcgttc
     2761 aacatgttga acaatatgat gcgggtatcc aaattgataa tggcgatgca catgccattt
     2821 ataacttcat caacactcac tcgagtaagg aattgcacga gatgggtgag cgcgcacatc
     2881 aactgtttaa agataaatat acgagagaaa ttaatactat gaagtattac aatctgttga
     2941 agtgaggaga taattatgaa gcgattattc gatgtagtga gttcaatata tggtttagta
     3001 gttttaagtc cgattctgtt aattacagca ttactaatta aaatggaatc acctggacca
     3061 gccattttca aacaaaaaag accgacgatt aataatgaat tgtttaatat ttataagttt
     3121 agatcaatga aaatagacac acctaatgtt gcaactgatt taatggattc aacatcgtat
     3181 ataacaaaga cagggaaggt cattcgtaag acctctattg atgaattgcc acaattattg
     3241 aatgttttaa aaggagaaat gtcaattgta ggtcctagac cagcgcttta taatcaatac
     3301 gaattaatcg aaaaacgtac aaaagtgaac gtgcatacga ttagaccagg tgtgacagga
     3361 ctagctcaag tgatggggag agatgatatc actgatgatc aaaaagtagc gtatgatcat
     3421 tattacttaa cacatcaatc tatgatgctt gatatgtata tcatatataa aacaattaaa
     3481 aatatcgtta cttcagaagg tgtgcatcac taatgagaaa aaatatttta attacaggcg
     3541 tacatggata tatcggtaat gctttaaaag ataagcttat tgaacaagga catcaagtag
     3601 atcaaattaa tgttaggaat caattatgga agtcgacctc gttcaaagat tatgatgttt
     3661 taattcatac agcagctttg gttcacaaca attcacctca agcaaggcta tctgattata
     3721 tgcaagtgaa tatgttgctg acgaaacaat tggcacaaaa ggctaaatct gaagacgtta
     3781 aacaatttat ttttatgagt actatggcag tttatggaaa agaaggtcag gttggtaaat
     3841 cagatcaaat tgatacacaa acaccaatga accctacgac caactatggt atttccaaaa
     3901 agttcgctga acaagcatta caagagttga ttagtgattc gtttaaagta gcaattgtga
     3961 gaccaccaat gatttatggt gcacattgcc caggaaattt ccaacggtta atgcaattgt
     4021 caaagcgact gccaatcatt cccaatatta acaatcagcg cagtgcatta tatattaaac
     4081 atctgacagc atttattgat caattaatat cattagaagt gacaggcgtg tatcatcctc
     4141 aagatagctt ttactttgat acatcgtcag taatgtatga aatacgtcgc caatcacatc
     4201 gtaaaacggt attgatcaac atgccttcag tgttaaataa gtattttaat aagttgtcgg
     4261 tctttagaaa attattcggc aatttaatat acagcaatac gttatatgaa aataataatg
     4321 cacttgaagt tattcctgga aaaatgtcac ttgttattgc ggacatcatg gatgaaacga
     4381 caaccaaaga taaggcataa gtcatctatt aaataaaatc aacatacaaa tcgttttatt
     4441 tggaggttat agtatgaagt taacagtagt tggcttaggt tatattggtt taccaacatc
     4501 aattatgttt gcaaagcatg gcgtcgatgt gcttggtgtt gatattaatc agcaaacgat
     4561 tgataagtta caaagtggtc aaattagtat tgaagaacct ggattacaag agatttatga
     4621 agaggtactg tcatcgggaa aattgaaggt atctacaacg ccagaagcat ctgatgtttt
     4681 tatcattgcc gttccgacgc cgaataatga tgatcagtac cggtcatgtg acatttcgct
     4741 agttatgcgt gcattagata gtattttacc atttttaaaa aaagggaata ctattattgt
     4801 agagtcgaca attgcgccta aaacgatgga tgattttgta aaaccagtca ttgaaaattt
     4861 agggtttaca ataggtgaag atatttattt agtgcattgt ccagaacgtg tactgccagg
     4921 aaaaatttta gaagaattag ttcataacaa tcgcatcatt ggcggtgtga ctaaagcttg
     4981 tattgaagcg ggtaaacgtg tctatcgcac atttgttcag ggagaaatga ttgaaacaga
     5041 tgcacgtact gctgaaatga gtaagctaat ggaaaacaca tatagagacg tgaacattgc
     5101 tttagctaat gaattaacaa aaatttgcaa caacttaaat attaatgtat tagatgtgat
     5161 tgaaatggca aacaaacatc cgcgtgttaa tatccatcaa cctggtccag gtgtaggcgg
     5221 tcattgttta gctgttgatc cgtactttat tattgctaaa gaccctgaaa atgcaaagtt
     5281 aattcaaact ggacgtgaaa ttaataattc aatgccggcc tatgttgttg atacaacgaa
     5341 gcaaatcatc aaagcattga gcgggaataa agtcacagta tttggtttaa cttataaagg
     5401 tgatgttgat gatataagag aatcgccagc atttgatatt tatgagctat taaatcaaga
     5461 accagacata gaagtatgtg cttatgatcc acatgttgaa ttagattttg tggaacatga
     5521 tatgtcacat gctgtcaaag acgcatcgct agtattgatt ttaagtgacc actcagaatt
     5581 taaaaattta tcggacagtc attttgataa aatgaagcat aaagtgattt ttgatacaaa
     5641 aaatgttgtg aaatcatcat ttgaagatgt atcgtattat aattatggca atatatttaa
     5701 ttttatcgac aaataaaatg tgtcaaacta gggcatacat gattaaggaa agataagctg
     5761 tcatgtgttt gaacttcaga gaggataatg ttatgaaaaa aattatggtt attttcggta
     5821 cgagacccga agcaataaaa atggcaccat tagtaaaaga aattgatcat aatgggaact
     5881 ttgaagcgaa cattgtgatt acagcacaac atagagatat gttagatagt gtgttaagta
     5941 tatttgatat tcaagctgat catgatttaa atattatgca agatcaacaa acgttagcgg
     6001 gccttacggc taatgcgctt gctaaacttg atagcatcat taatgaggaa cagccggata
     6061 tgattttagt acatggtgat actacaacga cttttgtagg aagtttggca gcattttatc
     6121 atcaaattcc ggtcggacat gtagaagctg gacttcgaac acatcagaaa tactcaccat
     6181 ttcctgaaga gttaaatcga gtcatggtaa gtaatattgc tgaattgaat tttgcgccaa
     6241 cagtaattgc agctaaaaat ttactttttg aaaacaaaga caaagagcgt atctttatta
     6301 ctggaaatac agttattgac gcattgtcaa caacagttca aaatgatttt gtttcaacga
     6361 ttattaataa acataaaggc aagaaagtta ttttactaac agcgcatcgt cgtgaaaata
     6421 ttggggaacc gatgcatcag atttttaaag cagtaagaga tttggcagat gaatataaag
     6481 atgttgtctt catttatcca atgcatcgta atccaaaggt aagagcgatt gccgaaaaat
     6541 atttatctgg gagaaatcgg attgaattaa ttgagccatt agatgcgatt gagttccata
     6601 attttacaaa tcaatcgtac ctcgtgctga cagattctgg tggtattcaa gaggaggctc
     6661 ctacatttgg aaaacctgtg ttggtattaa ggaatcatac agagcgtccc gaaggcgttg
     6721 aggcgggaac atcgagagta attggcacag attatgacaa tattgttcga aatgtgaaac
     6781 aattgattga ggatgatgaa gcatatcaac gtatgagtca agcgaataat ccatatggtg
     6841 atggacaagc atcacgacgt atttgtgaag caatagaata ttattttgga ttgcgcacag
     6901 acaagccgga tgaattcgta cctttacgtc acaaataata aaaaacccct aatcatgaag
     6961 ttggtttaga caaccagcgg tgactagggg tttttaatat atttattttt gatagtggta
     7021 gccaatatca tatttgaata ctttatttga taatattgga ctttgctgtc catcgtcatc
     7081 actttttaaa cgtacatttt tatgagcttc tttaaataca tcggaattca accaattatt
     7141 aaagctatct tcagattccc aaatagttaa gattttaact tcgtctgtat cctcagtatt
     7201 taatgtttta gtgacaaaca tttgttggaa gccttcaata gtttcaatgc cttgtctatt
     7261 gtaaaaacgt tcaatcgttt cttccgcact gcctttttgt aattgtaatc tattttctgc
     7321 cataaacatg ggcaatcact cctctatttt atgatttgat ttgggtaatg tttttacaaa
     7381 tgtaaagagt acagcggttt gtatgataac cattatgatt aatcctatac gtactgcaag
     7441 aacatccacc atataaattg aaaaacctat tacaatgtat aagctaatta aaattttaat
     7501 tttctgttgt agcgtgtagc ctcgatgtaa ataaaagttt tctacatatt ctttataaat
     7561 tttttgatta ataagccaat tgtaaaagcg atctgaactt cgagcaaaac aaaaaactgc
     7621 tacgagtaaa aaaggggtcg ttggcagtaa aggtaatacg gcacctgcaa taccaagcgc
     7681 tgtaaatatt aagccaatga cgattaaaat aagtcgcatt gaaaaaactc cattctagta
     7741 ctaatgcgca tgtaatattg ttttagtaat ataactcatg ctaaatataa tgtgtatgat
     7801 aagtgcaatg actcagtaaa atgaaacgat gttgaattat ccttgtcaca ttaacgcatt
     7861 ttaagcgcga ctttcataac aaccaaacta tttaatgaga attattctca agtattatag
     7921 ttatattatg tgttttattt ttgaaaagtg caatatgttt tcgaaaataa gattattttt
     7981 atgtgcaaaa acgacgcaaa agttttaaaa atgagacttc tgtgagctga ttattttata
     8041 aaatgtaaac gcttactata taatgtgaat catatcgttt aaaagcatta ttaaatatga
     8101 tgctaagaga tttatattat agccaataaa caaaggagag ataatatggc agtaaacgtt
     8161 cgagattata tagcagagaa ttatggttta tttatcaatg gggaatttgt taaaggtagc
     8221 agtgacgaaa caatcgaagt gactaatcca gcaactggag aaacactatc acatattaca
     8281 agagcaaaag ataaagatgt cgatcatgca gtcaaagtgg cgcaagaggc atttgaatca
     8341 tggtcattaa cttctaaatc agaacgtgca caaatgttgc gtgatattgg tgataaatta
     8401 atggcacaaa aagataaaat tgcaatgatt gaaacattaa ataatggtaa accgattcgt
     8461 gagacaacag caattgatat tccatttgct gcaagacatt tccattattt cgcaagtgtt
     8521 attgaaacag aagaaggtac agtaaatgat atcgataaag acacaatgag tatcgtacga
     8581 catgagccga ttggcgtcgt aggtgctgtt gttgcttgga acttcccaat gctattagct
     8641 gcatggaaga ttgcgccagc cattgctgca ggtaatacaa ttgtgattca accttcgtct
     8701 tcaacaccat taagtttatt ggaagttgct aaaattttcc aagaggtatt acctaaaggt
     8761 gttgtcaata tactaacggg taaaggttca gaatcaggta atgcaatttt caatcatgat
     8821 ggtgtagata aattatcatt tacgggctca actgatgtag gttatcaagt tgccgaagct
     8881 gcagcaaaac atctagtacc cgctacatta gagcttggtg gtaaaagcgc caatatcata
     8941 ttagatgatg ctaatttaga ccttgcagtt gaaggtattc agttaggtat tttattcaac
     9001 caaggtgaag tatgtagtgc aggttctcga ttattagttc atgaaaaaat ttatgatcaa
     9061 ttggtgccac gtttacaaga ggcattttca aatattaaag ttggagatcc acaagatgaa
     9121 gctacacaaa tgggtagtca aactggtaag gatcaattag ataaaattca atcatatatt
     9181 gatgcagcaa aagaatcaga tgcacaaatt ttagcaggtg gtcatcgctt aactgaaaat
     9241 ggattagata aagggttctt ctttgagccg acattaattg ctgtgccaga caatcatcac
     9301 aaattagcac aagaagaaat atttggacca gtgttaacag tgattaaagt gaaggacgat
     9361 caagaagcaa ttgatatagc taatgattct gagtatggtt tagcaggcgg tgtattttct
     9421 caaaatatca cacgtgcatt aaatattgct aaagctgtac gtacaggacg tatttggatt
     9481 aacacttaca accaagtacc agaaggcgca ccatttggtg gttataaaaa atcaggtatc
     9541 ggtcgagaaa cttataaagg tgcgttaagt aactatcaac aagttaaaaa tatttatatt
     9601 gatacaagca atgctttaaa aggtttgtac tagaataaat atcgtttctg aagcgtgttt
     9661 gtaggtcagt ctagcggtaa gtcttaacat ttaacggcgt tgtttagatt ttaagcaaaa
     9721 caaaatatat aggaacacgt atcatgatat taggatataa tgactaaaat aatagcagta
     9781 ggatggtttt taattgcaaa tcatcttact gctgttttta attatgctaa tttgcgatgc
     9841 ggctattata aggacagagt tgtttattaa ttatggtgat ttagaaatat gaagttcaat
     9901 atgcaaagtc atcgtttgtt ttaatatgcg gaacaatcat taaagttatt gcgatttttt
     9961 gaacttaatg aaactaaaca ataaatttga gatacttttt tgtcattttt atgtaactaa
    10021 cacaataatc tcgtacatta ttaaaatttt ctatatgata ggaataaagc aaagagcgag
    10081 tgtgctgtaa aagttttcca aggtgatatt acataaagct ataaagggta aagattaatg
    10141 agttgtcatg taaatgacga tgatgtataa atcatggtta attacggaag cattaatatt
    10201 aacctgagaa gctataaaga attattttta aaagcgacaa tattaaatac gacgcattta
    10261 tttaggagtg gcaaacgtat gaatgggaaa aaggcgaata cgataaacag atacaaatat
    10321 tttcatcatg tcaatcatca aaaaattcaa caaagttcta aaaagacgct gtgggcatca
    10381 cttatcatca cattgttatt tacagtgatt gaatttgtcg gaggtttagt atctaattca
    10441 ttggcattac tgtcagattc atttcatatg cttagtgatg tattagcact tggtttatct
    10501 atgttggcca tttattttgc aagtaaaaag ccgactgcac gatacacatt tggatattta
    10561 agatttgaga tattagctgc atttttaaat ggtttagcat taattgtaat ttcaatctgg
    10621 attttatatg aagctattgt acgtattatt tatccgcaac caattgaaag tggcattatg
    10681 tttatgattg ctagtattgg tttactcgtt aatattattt tgacaattat ccttgtaagg
    10741 tctttaaaac aagaagacaa tatcaatatt caaagtgcat tatggcattt catgggagac
    10801 ttattgaact ctattggtgt catcgttgca gttgtattga tttactttac aggatggcgc
    10861 atcatcgacc caatcattag tattgtaatt tcactcatca ttttacgtgg tggttataaa
    10921 attacgcgta atgcgtggtt aattttaatg gaaagtgtgc ctcaacattt ggatactgat
    10981 caaattatgg cagatattaa aaacatagat ggcatattag atgtacatga atttcatttg
    11041 tggagtatta caacagagca ttattcatta agtgcccatg ttgtgttaga taaaaaatat
    11101 gagggtgatg attatcaagc gattgatcaa gtatcatcat tgttgaaaga aaaatatggc
    11161 attgcacatt caacgttgca aattgaaaac ttgcaattga atccattaga tgagccatac
    11221 ttcgacaaat taacataaat aaaacattgt acaggctggg acataaatca atgttctatg
    11281 ctctacgaag ttatattggc agtagttgac tgaacgaaaa tgcgcttgta acaagctttt
    11341 ttcaattcta gtcaggggcc ccaacacaga gaatttcgaa aagaaattct acaggcaatg
    11401 caagttgggg tgtgggcccc aacacagaga atttcgaaaa gaaattctac aggcaatgca
    11461 agttggggtg tgggccccaa caaagagaaa ttggattccc aatttctaca gacaatgcaa
    11521 gttggggtgg gacaacgaaa taaattttgc gaaaatatca tttctgtccc actcccacgt
    11581 ttgttttgga ttatgttgca tcatttaaat gattttcgtc aatttctttg acgctatcta
    11641 catctaacac gacatcttta ggtttcaaaa tatgaatatg tttttcatca tttgtatgta
    11701 aaatgcgttc tatgatgtat ctttgaccgg ccattgtttc tacagcaatc tttttgtttc
    11761 tagctaaact tgctacgaca gattctttat
//
LOCUS       DBNENW010000078        11766 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNENW010000078 DBNENW010000000
VERSION     DBNENW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.078"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(43..255)
                     /locus_tag="AC1LW8_001679"
     CDS             complement(43..255)
                     /locus_tag="AC1LW8_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773509.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            complement(285..896)
                     /gene="pyrE"
                     /locus_tag="AC1LW8_001680"
     CDS             complement(285..896)
                     /gene="pyrE"
                     /locus_tag="AC1LW8_001680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIP4773510.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYSELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(896..1588)
                     /gene="pyrF"
                     /locus_tag="AC1LW8_001681"
     CDS             complement(896..1588)
                     /gene="pyrF"
                     /locus_tag="AC1LW8_001681"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIP4773511.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(1698..4871)
                     /gene="carB"
                     /locus_tag="AC1LW8_001682"
     CDS             complement(1698..4871)
                     /gene="carB"
                     /locus_tag="AC1LW8_001682"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIP4773512.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(4864..5964)
                     /locus_tag="AC1LW8_001683"
     CDS             complement(4864..5964)
                     /locus_tag="AC1LW8_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIP4773513.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(5966..7240)
                     /locus_tag="AC1LW8_001684"
     CDS             complement(5966..7240)
                     /locus_tag="AC1LW8_001684"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIP4773514.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(7258..8139)
                     /locus_tag="AC1LW8_001685"
     CDS             complement(7258..8139)
                     /locus_tag="AC1LW8_001685"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIP4773515.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(8167..9474)
                     /locus_tag="AC1LW8_001686"
     CDS             complement(8167..9474)
                     /locus_tag="AC1LW8_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIP4773516.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(9692..10219)
                     /gene="pyrR"
                     /locus_tag="AC1LW8_001687"
     CDS             complement(9692..10219)
                     /gene="pyrR"
                     /locus_tag="AC1LW8_001687"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIP4773517.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(10619..11536)
                     /locus_tag="AC1LW8_001688"
     CDS             complement(10619..11536)
                     /locus_tag="AC1LW8_001688"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP4773518.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKTKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(11536..>11766)
                     /gene="lspA"
                     /locus_tag="AC1LW8_001689"
     CDS             complement(11536..>11766)
                     /gene="lspA"
                     /locus_tag="AC1LW8_001689"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIP4773519.1"
                     /translation="YNLFMQVAISLLFAGALGNFIDRILTGEVVDFIDTNIFGYDFPI
                     FNIADSSLTIGVILIIIALLKDTSNKKEKEVK"
BASE COUNT         3555 a         2299 c         1713 g         4199 t
ORIGIN      
        1 gttaagcagc ccctgatatt aaacactata gcttttttgt ttttaaattt gtccttgttt
       61 acgtaatata tcctttacat gtttaaataa atcatcatta tggtctatag ttgttttatt
      121 ttttatatgt ttttgcttcg ttgaactttc gtcgacatcg tgactactag atagattgga
      181 atcatttgaa tgattgataa attcgttaca atgttttatc ttcccatata aatcattaga
      241 tgttttgtca ttcatttgtt gaattcctcc ttctagtgtc tatattatgc taagttgtct
      301 ctccattcaa ctaatgtttg gatatcttca ctagaaattt taccttcatt ttcagctact
      361 tcaattaatt cactgtaatc acttaaagtg taaaaaggta gttgaatatt gctaaatgta
      421 tcatctgctt ttttcaaacc gtaagtaaag atagctacaa cacctaatac ttctgcacct
      481 gctagtttta aggcttcaac tgctgtgact gaagatcccc ctgtcgaaat taaatcttct
      541 atcacaacta cttttttacc ttcactttta gcaccttcga tttgattttg cttaccatga
      601 ctcttatttg atgaacgaac ataattcatt ggtaatttta atttttcagc aataaaagct
      661 gcatgtggaa taccagctgt tgcagtacca gaaataactt ctacttcagg aaagtgttct
      721 ttaattaagt taattaaacc atcgcggatt gcgcctcgaa ctaaaggata acctaacgta
      781 acacggttat cacagtaaat cggtgattta atacctgaac tccatgtata taaatcattt
      841 ggtgataatg ttacagcttc aatatctaat aatgattttg caatttcttt agccattata
      901 ctaaccaact ttctttaatt ttatgataac tttcgactgg attgtcactt tgtgtaatcg
      961 gtctaccgac tacaatatgc gtcgaaccaa gctgtcttgc ttcttccggt gtcgtaatac
     1021 ggtgttggtc atcttgagat gcacctttag gtctaatacc tggtgttact tttaaaaatg
     1081 atgtacctaa cttttcagtc aacatacgac tttcaagagg tgaacaaaca acgccatcta
     1141 aaccagctgc atttgctaac ttggcataat ttaaaacggc ctcttcaatc gatgtttgta
     1201 tattttgttc gtgtcgtaac atgtcttctg ttgttgacgt aagctgtgtt actgcaataa
     1261 tttttgtatg ttgattatgt tttcttaatc ctttaatggc ctcagacatc atttttacgc
     1321 caccagcagc atgaacattt accagatcaa cattcaattt agctagtcct tccatcgcct
     1381 tacctactgt attaggaata tcatgcaatt ttaaatctaa aaatacatca tggcctcttt
     1441 cttttatctc attaattaat tgaggacctt cttgataaaa aagttccata cctactttta
     1501 cgaataatga ttcatcaaat aaatctaaaa attgatttac tttttctttt gattcaaaat
     1561 ctaatgcaat aattggtaaa tctttcatca tatatgtctc cctttatatt aagaaattca
     1621 ttaaaataat gaggctgtaa tataaacttc tttaagtaaa tggttaatta cagccccacc
     1681 gatacagttt gattgattta catttgacgc attgtaaatg tcatactttc aattacattc
     1741 gttaaggcat tagctgtatc taaagatgtc aaacatggaa taccattttc aactgtagta
     1801 cgtctaattt ggaagccatc tctttctact tctttacctt tagtcattgt atttataacg
     1861 atttgaacat caccattttg aatacgtgtt aataaatcat tttcgccacc aattttgcct
     1921 actacttctg caggtatgtc atactcagct aatttattag ctgttccaga cgttgctaaa
     1981 attttatagc caacttcatt taagcgttgt gccaatttaa caacttcctc tttatctttg
     2041 tcactgacgg tcattaatac tgtaccgtga tctttaactt caacaccact acctgttaac
     2101 cctttgaata acgccttttc taatgtagta tctttcccca tcacttcacc tgttgacttc
     2161 atttcaggtc ctaaagtaat atcaacattt ttcaatttat tgaaactaaa taccggtgct
     2221 ttcacaaaga caccctcagc atatggttga accccttctt gataacccat atctgttagt
     2281 ttttccccaa tgattgctcg catagctaat tgtgccattg gaatatcagt aattttactt
     2341 aagaatggta ccgttctact agaacgtgga tttacttcta aaacatacac accatcgtga
     2401 gctataacga attgaatgtt gattaagcca atgatgttta aacctttagc taattttata
     2461 gtatagtcct caagtgttgc taactcgtct tctgtcaaag tttgtggtgg atatacagcg
     2521 attgagtcac cactatgcac accagcacgt tcaatatgtt ccattattcc tggaataatg
     2581 accgtttctc catcacaaat cgcatcaact tcaatttctt taccagttaa atatctatcg
     2641 actagtaccg gatgttccgg actcgctttt acagcctggg tcatatagtt ttctaactct
     2701 ttgtcattgt ctacaatttc cattgcgcga ccacctaata cataagaagg tcttactaca
     2761 accggatatc cgatttctgc agcattcgct aatgcttcct caggtgatgt agctgttttc
     2821 ccttgtggct gtggcacgtt aatttttctt aatagtgctt caaattcttt tctatcttca
     2881 gcacgattta gattttctag tgaagtacca agtattttaa caccatgttt agccaatttg
     2941 tctgctaaat taatcgctgt ttgtcctcca aattgtacaa cgacaccttt aggtttttct
     3001 aaattaatga tattcatcac atcttcttca gttaaaggtt caaagtataa tttgtcagaa
     3061 attgagaagt ctgttgaaac tgtttctgga ttgttattca caattatcgc ttcgtaccct
     3121 gctttttgaa ttgcccaaac ggcgtgaact gtcgcatagt caaattctac accttggccg
     3181 attcgaattg gtccagagcc taatactaag attttttctt tgtcagtaac tatggattca
     3241 ttttcagttt cgtatgtacc ataataatat ggtgttgaag attcaaattc agctgcgcaa
     3301 gtatcaacca tcttgtaaac aggtttaata tcattttcca tacgcaattg atatacttct
     3361 tcttccgtca tattaaagcg atgcgctatt gttttatcac taaatccata atcttttgca
     3421 tatttaagat attctaaatc accttgatgc tcttttagtt gatgctcaat atcaataatg
     3481 ttttggaact tgtgtaagaa gaagtaatca atctgagtca tattatgaat ttcttctaat
     3541 gttgtgcctc ttctaattgc ttcgccgatg aaaaataatc gttcatcatc ttggtgtgaa
     3601 atacgttctt taatataatc aagatcgaag ctttcaccat ttggtaatcc taagtgatgc
     3661 acaccatact caagtgatcg aattgctttt aacaatgatt cttcgtaagt tcgaccaatg
     3721 gccataactt cacctgttgc tttcatttgt gtgccaagct ctcgttctcc tttttcaaat
     3781 ttatcaaaag gaaatcttgg tatttttgaa atcacatagt ctaaagttgg ttcaaacgct
     3841 gcataagatg ttcctgtaat tggatttaac atttcatcta atgttagacc aaccgcgatt
     3901 ttagcagcta attttgcaat aggatatcct gttgcttttg aagctaacgc tgatgaacgt
     3961 gatacacgcg gatttacttc tataatataa taatcgaatg aatgggggtc taatgctaat
     4021 tgaacattac aaccaccttc gatacctaaa gctcgaataa cttttaatga aacatcacgt
     4081 aacatttgat actcaacatc tgataatgtt tgactaggag ccacaacaat tgaatcgcca
     4141 gtatgaatac caactggatc aatattttcc atgttacata caacgatggc attatcgttt
     4201 ttatcacgca ttacttcgta ttcgatttct ttaaaacctg cgatagattt ttctaataaa
     4261 cattgcgttg ctggactata atgaagacca tttgagacga tttcatgtaa ttcttcatca
     4321 ttatgacaaa taccgcctcc ggtaccaccc atcgtaaatg ccggtctaac aattagcggg
     4381 tatcccactt gctctttgaa tttaaaggct tgctctactg tatttacaat gtcactctct
     4441 ggtacaggaa cgtttaaatc attcattaat gttctaaaca tttcacggtc ttctgcttgt
     4501 tgaattgatg ttagctcagt tcctaataat tggacgttat tatcttgaag cacaccactt
     4561 tcgtgtagtt gaatcgccat gtttaaacct gtttgaccac ctaaagttgg aagtaaagcg
     4621 tcaggttgct ctttacgtat aattcgcgct ataaaatcat gagttaacgg ttcgatatat
     4681 actttatccg caatttcctt atcagtcatg attgtcgctg gatttgaatt tacaagaata
     4741 actcgatatc cctcttcttt taaagctaga catgcttgtg ttccagcata atcaaattca
     4801 gctgcttgac ctatgataat tggcccagac cctattacta aaattgtttt gatatcatta
     4861 cgtttaggca ttgatatgac gctccttttc cttaaaatta gtcatcattg ctacaaaatc
     4921 atcaaataga tagtttgaat ctgacggtcc tggatttgct tcaggatggt attgaacaga
     4981 aaatgctggt aatgttttat gttttaaacc ttctacagta ccatcattta atgcaagatg
     5041 agtaacttct aaatcagtac tttttaacga atctatatct attgcatatc catggttttg
     5101 actcgtaata tcaacttttc cagtctctaa atttttaact ggatggttcg caccacgatg
     5161 accaaacttc attttaaatg agcttgctcc ttgagataat gcaaaaagtt gatgtcctag
     5221 acagatacca aagaacggaa ttttccctaa aattccttga atcattggaa tcgcacattc
     5281 tacaacttca ggattacctg gtccgtttga tagcataacg ccatctggag ccattgctaa
     5341 aatttcttcg gcagtagttg tatatggtac aactgtgacg ttacaacctc tgacgtttaa
     5401 ttctcgaaca atattttgct tcttaccaaa gtctacaagt acgacactta gatccttacc
     5461 tgtcgaaaca tacggtgttt tagtcgaaac gatttctact tcgtttttag gtaattctac
     5521 ttgttgtaaa tgtttgacaa gttgatcaat atcttctttt cgatcagtaa aaccagcttt
     5581 taacacaccg tgttgtcgaa ttttacgcgt aatacttctt gtatcaacac ctgcaatccc
     5641 tggaatttga tgcaattcta aaacgtcatg aagtgtcttt tgttgtctaa aattacttgg
     5701 atgcgcactc gcttctttca ctacaatacc gtttaatgta ggtactaatg attcaaaatc
     5761 gtctctattg ataccataat taccgattaa tggatacgta aaagtaatga tctgacctgt
     5821 atatgatgga tctgaaatag tttcttgata acctgtcatc gctgtattaa atacaatttc
     5881 tcctacagtt aagttatcag atcctaaacg gtagccttcg taaaaagaac cgtcttctaa
     5941 cactagataa cgtttgcttt gcatattatt tatccccctc aaatttaact tcgccttcaa
     6001 ccattgttaa gatcggattt ccataaactt tatagccgat aaatggtgta ttatctgctt
     6061 ttgataagaa atcttctcct ttaatttctt gttcactatc taaatcaatg attgttaaat
     6121 ctgcataacc attttctttt aatgtgccgt attctaaatt aaatgtctca catggtttaa
     6181 ttgttaagta atctactaat tgttgtaatg tccaatcacc attttttaca aaatgcgtat
     6241 ataataatgg gaatgctgtt tcactaccaa caattccgaa tggtgctttt tccattggtt
     6301 gcgctttttc atcacgtgca tgtggtgcat ggtctgttgc gatacaatca attgtaccgt
     6361 ctagtaaccc ttctaacaaa gcctctctat cttcagtact tctcaatggt ggattcattt
     6421 tataaatggc attattacca ggaatatcat cttctgttaa caataaatga tgtggtgtaa
     6481 cttcagctgt aacatgaatg cctgcgcgtt tagcatcacg aatgactcta acactttctt
     6541 tagtagaaac atgacataca tgataatgac aacctgctgc ttcagctaat agtacatctc
     6601 ttgcgatttg aacagattca caaatgtttg gaatacctgg tatacctaac tctttactgc
     6661 gtttcccttc atgcattgca ccaccataga ttaatgaatt atcttcacag tgtgctacga
     6721 tggctttgtt tacttttgca gcttcaatca taccttcata catcatgctt gcagtttgta
     6781 ctcctacacc gtcatctgta aacgcaaagg caccttcttt tactagtgct gggaaatcaa
     6841 ccaattcttt acctaattga cgtgttgtaa ttgaagcata aggtaataca cgtacttgag
     6901 cattgtcatc gattaatttt tgtaaagctt caaaatgttc tacagaatct ggtaccggtc
     6961 ttgtgttagg cattggacat acagttgtaa atccgcctct agcagcagct ttagtaccag
     7021 tttcaattgt ctctttatat tcaccaccag gttcacgtaa atgaacatga acatcgacaa
     7081 atccaggtga cacaaagtga cctttcgcat ctatgatgtc aacaccattg cttggttcaa
     7141 ttgcaggtgc aatttgttta attaccttac catcaattaa aatatctgct tgttgtaatt
     7201 cgccattttg taatacttta ccgtttttaa ttaatttcat cattttcgtc cccttaccta
     7261 ttttaataat tcatcaatga ctgccattct taagtaaacg ccattttcca tttgcttaaa
     7321 aattcttgat tttgaagctt ctactaaatc gctttgtatt tctactcctc tattcacagg
     7381 tgccggatgc ataacaatag catgttcttg taatttgtta tagcgcactt catttaagcc
     7441 atgcttttga tgataatcat ctgctgcaaa tctagtttct tctgcaagcc catgtctttc
     7501 atgttgaatt cttaataaca taactatatc tactgtttct ataacatcat ctatatttac
     7561 ataaggtgct tctaaagaat catcaatcca agcatttggg ctattaaaca ttacgtttgc
     7621 acctaatgct tttaaactat ggtaattact acgtgcgaca cgtgaatttt taatgtctcc
     7681 acaaatcaat acattcaagc cttcaaaata tccatattct tcatatatcg tcattaaatc
     7741 aagtaaactt tgtgttggat gttgtccact accatcacca gcattcgcaa ttgggatgtt
     7801 aatattcgct aatttttcat agtagttatt aaacggatgt ctaatgacta ataaatcaca
     7861 gccaatactt tctaaagttt tacatgtgtc atataaagat tcaccttttg aaacagatga
     7921 tgttgatgtt tcaaagctaa tcgtttttaa ccctagctta agttctgcca tttcaaaact
     7981 acattttgtt cgagtagaat tttcaaagaa taaatttgcg acatatttcc cttcaaagtt
     8041 tggtagttga cgttcaccag atttaaattg acttgccttt tggataagtt tgtatatttg
     8101 atctgtagat aaatgttcca ttgataataa atgattcata aaagcgccct ccttaatttg
     8161 taaatcttaa ttgttttgtt ttttctcttt aggtaagatt aagttcaaaa taattcctga
     8221 aagtgcagct aatgccatcc cctcaatttg aaggttgata ccaatttctt ttaagttaaa
     8281 tactaaatta ccgataccta caactaaaat tactgaagct ataactaaat ttcgattgtt
     8341 cgcaaaatct actttgcttt caactagcat tcttaagcca cttgctgcaa taataccgaa
     8401 aagtaatata gatactcctc ccataactgg tgtaggtata gaagaaatta atgcagtgaa
     8461 cttaccaatg aatgctaaaa caattgctat aactgctgca ccaccaatga cgtaaatact
     8521 gtatattctg gtaatcgcta atacaccgat attttcaccg tatgttgtac ttggtggtcc
     8581 accaataata ctggcaaaca ttgtagaaac accatcacca atgattgatt tatcaagtcc
     8641 tggcttttca aagaagtttc tacctacgat tttattcaat accatttggt gcccaatatg
     8701 ttcacttact gttacaaaca caatcggaat cattaccagt acaagtccta agtgaaatga
     8761 aggtacataa tctttaaatg gcagataaat atgagggaaa tctatccatt ttgctgacat
     8821 aattgcatca aatttcacaa tccccataaa aatcgctacc acataaccta cgataatacc
     8881 tacaagtact ggaattaaag ataggaatcc tttaaaaaat ccttgaacga ctattgttac
     8941 aagcaaggtt atcattgcaa caattaagaa actgatattg taacctttca tatctccagg
     9001 attttcatac attgccatat tgactgcagt aggcgctaag cttaaaccaa ttaccatgat
     9061 gactggtcca acaacaactg gtggtaataa tttcattaac catgctgtcc cacttaattt
     9121 gattagaatc ccgatgatga cgtacataac accactcatg aataatgcta caagcatgtc
     9181 tcctaagcta tgcgtactta atcccgtgat aattggcgtg ataaatgcaa agctagatcc
     9241 caagtatgct ggtatttgcg ccttcgttat taagatataa agtaatgtac cgattcccga
     9301 agctagtaac gctgcggata ttggtaatcc tgttaagaat ggtactagta ctgttgcgcc
     9361 aaacatcgca aataaatgtt gtaagcttaa aaatgcccat tgcgctggtt gtggtttttc
     9421 atttacatct agtacaggtt ttactgttcg ttcaaacatt tcatcatttt gcataatatt
     9481 catttcctcc gataataaaa aaatctcttt acatcagtat atgtaaagag acaaaaagtg
     9541 tgacaagttg ctacaagtca ttttcgtcca tagaaattga cttatagttg tcgaacatga
     9601 gggtattatt agataaacaa gcatatgaaa acttatttat cattcaactc ccccaccttt
     9661 ttcagtctct cgtactgaat taaaaggggt attatttaat tataactgca tttctttgat
     9721 ccatttcttc taaatagaca cttaccgttt cctctttaga agtaggtata tttttaccaa
     9781 caaaatctgc tcgaattggt aactcacgat gtcctcgatc aaccaaagca gctaaaccaa
     9841 ttttaatagg tctagcattt agcaaaatag catcaagtga agcacgaacc gttcgaccag
     9901 tatacagcac atcatcaata atgatgacta ctttatctgt aatatctgtg tcgatgtcta
     9961 ttgcgtcttt tgtcgtaagt gatgacatgt gctctatatc atctctaaag tatgtaatat
    10021 caattgttcc agtaggtata cgttgttgct caatttgatg aattttatct tgtatacgat
    10081 tcgctaaata ttcacctctt gttttgatac ctaaaagaat taaattatca gtacctttgt
    10141 tatactccaa aatttcgtga gcgattctcg tcactgtacg ttgtattgcg gcatcatcca
    10201 ttatgatacg ttcagacata ttctcaccct ttcaaaattt agaaacaaaa aacctcatgt
    10261 ctttaatatc gacatgaggt ttagtatata caatcagcct atttacatag aagaatcgta
    10321 gtattactac ttattcttgc atatgtgaaa gtgttatgat cattttagta tatatctata
    10381 ttttcatgtc tttgacgtct ctctggacgc tcaattaaag actctttatg ttgtacttaa
    10441 actgttataa ggataatact aatcaggcgc tttgtcaatc tatattgaaa aattaaccga
    10501 tctcagtaaa aatgttaacc ttaatctatt ttataatgtg ttttcatttt aaaaaaacga
    10561 aaacgataaa tacttatagt ttacataata atgttatcgt aattatagca caacacaatt
    10621 atgcatctct ttttcgaatt gtatctaata aatcttcaaa gtcttgtggt aattcagcat
    10681 gtctttcaat atattcacct gttactggat gttcgaatcc aataagtcca gcatgtagag
    10741 cttgaccacc aatatccaat gtctttttcg gtccatactt tggatcacca actaatggga
    10801 agccaatata tttcatgtgc acacggattt gatgcgtacg tcctgtttca agttgacatt
    10861 caacaagcgt ataatcttta aaatgttcta gtacgttaaa atgtgtcact gcttccttac
    10921 catcatcaac aacagccata gattgacgat catttttgtt tctaccaatt ggcgcatcga
    10981 ttgtaccgta atcatgagga atattcccgt gaactaaagc gatatatttt cttttaacag
    11041 atttatccat taattgttct acaagcccac gatgagcaat atcattttta gcaaccatta
    11101 ataaaccaga agtatccata tctatacggt gaacaatacc tggacgaatt tctccattaa
    11161 tacctgacaa atttttaatt tgatacatta aaccattaac taatgtattg gtataatgcc
    11221 ctggtgatgg atgaactacc atgcctttcg ttttatatac aactgcaaca tcgtcatctt
    11281 cataataaat atctaaattt aaattttcag gtagaatatc agcttcaacc acttcttttt
    11341 cagtgacaac tatatgatca ttaagtttca ctttataatt agatttaaca actttatcgt
    11401 ttgcaacgac taaacctgct ttaatccaat cttgtatctg gttacgagac caatcactat
    11461 ttaattcagg cagcaactta tctacacgca tacctgtttg ttctttatct gtaatgttaa
    11521 attcataagt ctccattact taacctcctt ctccttttta ttggaagtat cctttaataa
    11581 ggcaataata attaatatta caccaattgt taaacttgaa tctgcgatat taaatattgg
    11641 aaaatcataa ccaaaaatat ttgtatcaat aaagtcaaca acttctcctg ttaaaattct
    11701 atcaataaag tttccaagtg cacctgcaaa aagtaaacta atagcaactt gcataaacaa
    11761 attata
//
LOCUS       DBNENW010000079        11587 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNENW010000079 DBNENW010000000
VERSION     DBNENW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.079"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(91..399)
                     /locus_tag="AC1LW8_001690"
     CDS             complement(91..399)
                     /locus_tag="AC1LW8_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIP4773520.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDNMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(414..842)
                     /gene="ruvX"
                     /locus_tag="AC1LW8_001691"
     CDS             complement(414..842)
                     /gene="ruvX"
                     /locus_tag="AC1LW8_001691"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIP4773521.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(846..1106)
                     /locus_tag="AC1LW8_001692"
     CDS             complement(846..1106)
                     /locus_tag="AC1LW8_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIP4773522.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1169..3799)
                     /gene="alaS"
                     /locus_tag="AC1LW8_001693"
     CDS             complement(1169..3799)
                     /gene="alaS"
                     /locus_tag="AC1LW8_001693"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIP4773523.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIENQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     misc_binding    complement(3931..4108)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4142..6619)
                     /gene="recD2"
                     /locus_tag="AC1LW8_001694"
     CDS             complement(4142..6619)
                     /gene="recD2"
                     /locus_tag="AC1LW8_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIP4773524.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6621..7289)
                     /locus_tag="AC1LW8_001695"
     CDS             complement(6621..7289)
                     /locus_tag="AC1LW8_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIP4773525.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(7870..8988)
                     /gene="mnmA"
                     /locus_tag="AC1LW8_001696"
     CDS             complement(7870..8988)
                     /gene="mnmA"
                     /locus_tag="AC1LW8_001696"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIP4773526.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKIEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(8989..10131)
                     /locus_tag="AC1LW8_001697"
     CDS             complement(8989..10131)
                     /locus_tag="AC1LW8_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP4773527.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            10443..11456
                     /locus_tag="AC1LW8_001698"
     CDS             10443..11456
                     /locus_tag="AC1LW8_001698"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIP4773528.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT         3514 a         2247 c         1633 g         4193 t
ORIGIN      
        1 ttataagcgt taattattta tacattgctt aacttaaaaa aatactcttg cctccccatc
       61 ttaaggttag caagagtaaa atctttttaa ttattcttcc atttcagtat ttacaacttc
      121 ttcaatcatg tcccattctt catcagtttc gattggtact aacttaccac cgtcacctga
      181 ctcatctggt tcattgatca ttggtacaag ctcaatcata ttgtcttcat ctgattgagc
      241 accttcttca gctaagataa catactcttt tttgaattca ggatgataaa attctaaaac
      301 ttttcggtat aaaacttcat ttccctcttc atcgaataaa gttaataatt cttcttcgtt
      361 attaatttct agttgtgaat catgattatg ttcagtcata gtaaaatctc cttttaatgt
      421 agtgaatcta aatagccttg taaaataaat accgctgcca ttttatcaat cacttgtttt
      481 cttttttgtc ttgaaacatc tgcttctaat aatgatcgtt cagcagccat tgtgcttaat
      541 ctttcatccc acatcacaat ctcaatagaa ggataagctt ctaataattt ttctttatat
      601 gttaacgaag cttcgcctcg aaatcctatt gaattattca tgtttttagg tagtcctatt
      661 acgactgtac ccacattatg ttttttaata atgtctacta attggtcaat acctaattca
      721 ttattttctt cattgattcg gagtgtgtct aatccttgtg ccgtccaacc cattatatca
      781 ctaattgcaa ttcctaccgt tctactaccg acatcgagtc ctaaaatttt atgttgtaac
      841 ataaattatt tattttgctc ttttaaatag taagaaacaa gttcttccat aataacatct
      901 ctatcaatat gacgaatttg atttcttgct tcattttggc gtggaatata cgcagggtca
      961 cctgataata aataacctac aatttggttt acggcattat atcctcgttc atctaatgtt
     1021 cgataaacat tatttaaaac atctcttaca tcttgcgttg gaagttcttc atagtcgaat
     1081 ttcattgttt tatcaaagtt ttccatttgc gacactcctt taattacaaa tataactcac
     1141 tatcatcata caatattatg gctttaaatt atagattttt aatgtaatct ttaataaagc
     1201 ttaatgattt tgagatattt tcaggttgtg taccgccacc ttgagccata tctggacgac
     1261 cgccaccttt accaccaacg attggtgcca tttgtttgat aagatcaccg gctttaacgt
     1321 tatttgttaa agatttaggg acagttgcaa ccatcgatac tttatcatca acattacttg
     1381 caagaatgat aattgtatct tgtagtttag atttaaaatc gtccattgtc gagcgaattg
     1441 ctttcgcatt tggtacatcc acttcagtaa ccaatacttt atagccattg atttcttcaa
     1501 cttgattttc aatattaccc attttaagtg atgtgatttc tttgtcacgt tgctctaatt
     1561 gttttaataa tgctttttct tcatcttgta attgtgttaa cttatcgact acttgatcat
     1621 cagatttcac tttcagctgt gatttcatcg tattaaattt ctcttgaata tcttctaaat
     1681 ataagaaagc tgctttacct gttaatgctt caatacgacg cacaccagct cctgtacctg
     1741 actcacttac tattttgaat aagccaattt cagaagtatt gcggacatga ataccaccac
     1801 ataattcaat tgaaaatggt gccatattta ctacacgcac aacatcacca tatttttcac
     1861 cgaataatgc cattgcgccc atttctttag ctgaagcaat atccatttct tgaatgttaa
     1921 cgtcaatacc tttccaaatt tcttcattta ctaagcgttc aacttgatca atttcatcat
     1981 tagtcattgg accaaaatga gagaaatcaa aacgtaaacg atctgcttct actagtgaac
     2041 cagcttggtt aacatgatca cccagtactg atttcaacgc tgcatgtaat aaatgtgttg
     2101 cactatggtt cttttgaatg tcacgtcgat cattttggtt cacttcagca gacactgtag
     2161 cgccaacatt tacttggcca aattgtacta ctcctttatg caagttttga ccatttggtg
     2221 ctttggttac ttcactaaca gcaatttcaa aattgtcatt ataaacaata cctgtatccg
     2281 caacttgtcc accactgact gcataaaatg gtgtttccgt taacatgaag tatactgttt
     2341 cacccgcttc aacttgtgaa acttcttcac cattatatat caagtgtgtt agtgttgttt
     2401 gagctgtcgc agtatcataa ccaacaaaag tacttgcaga tgtaatattt ttcaatactt
     2461 cactttgaac ttgcattgat tgagaatttt gacgtgcttg acgtgcacga tcacgttgtt
     2521 gttgcatttc tgactcgaat gttgtcatat caactttcaa tcctgcttgc actgctattt
     2581 cttcagttaa ttcaattggg aacccgtacg tatcatacaa tttaaatgca tctttcccat
     2641 taatttcatt tgttgtcgct ttagcttttt taattaattc atttaaaatc gctaaaccat
     2701 cttctaatgt ttcatggaat cgttcttctt cagactttat aacacgctta atgaaatctg
     2761 ctttttcctt aacatttgga taatatggtt ccataatgtc tgcaacaata tcaacaagtt
     2821 tgtacataaa tggctcattg attcctaacg tttgactaaa acgaacggca cgacgtaaca
     2881 atcgacgtaa tacatatcct ctaccttcat tggcaggtaa tgcaccatca gaaattgcaa
     2941 atgcaatcgt acgaatgtgg tcagcaatta ctttaaatgc cacatcttgt tcgttgttta
     3001 ctaaatattg tttacctgat actttttcga tttcattcat tataggcata aataaatctg
     3061 tttcatagtt agtacgtaca ttttgagaaa ctgaggccat acgctcaagc cccatgccag
     3121 tatcaatgtt tttattaggt aatggtgtgt aactatgatc tttattatga ttgaattcac
     3181 taaatactaa gttccatact tcaagatagc gttcattttc tccacctgga tacatttctt
     3241 ctgccggatc gtcttgtcca tatgcttctc cgcgatcata gaaaatctca gtgtttggtc
     3301 ctgaaggccc ttcaccaata tcccagaagt taccttcaat gcgaataata cgactttctt
     3361 caagcccaat atctttatgc caaatgttgt atgcttccat atcttccgga tgaatcgtaa
     3421 cgtacaattt atctggctcc atccccatcc atttatcact cgttaaaaat tcccaagcaa
     3481 attcaatcgc ttcttgttta aaataatcac caattgagaa gttacctaac atttcaaaga
     3541 atgtatggtg acgcgctgtg aaaccaacat tttcaatatc atttgtacga atagcttttt
     3601 gagagtttac aattcttggc tttttaggtg tttcacgtcc atcaaaatat ttctttaatg
     3661 ttgctacacc tgaattaatc cataataatg tatcatcatc aattggcact aatggtgcag
     3721 aaggttcaac catatgtcct ttttcaacaa agaaatctag atatttttgt ctaatttcac
     3781 tcgcttttaa ctttttcatc ttttacacat cctatttact gtttttaaat taccattcca
     3841 taaaaattga tgacacagat agtcgatttg aaaaactagt ataaatcaat atcatttttt
     3901 attattaaaa aataaaaaac gcccatcctc aaaagggacg aacgttatcg cggtaccacc
     3961 ctagttataa atgcaattca acacatttat cactttaaat cgactataca gttgtgcata
     4021 aagtagcgtt cactaatgtt tgttgtactt ttcaccaacc agtacatctc tgataaacaa
     4081 atcattaact actcatcttt atacgaattt aattctattt tagttacatt tactcttgtt
     4141 gtcaacgttc tataaagtca tacggcgtga tttctcccat attaatcatt gggtcaattt
     4201 taaacattgt agcttccgtt aatacattta tatctgtttt tgttgaatca gacgtaactt
     4261 ctccactatc attcgatgac attggcgctt ctacttgatc atctattgtc gtttgtgaag
     4321 ctcctgtatc attagttgct gtgttttcca gcatttcttc atcttctgaa ttaaaataat
     4381 ttttcaacaa tgtacataat tgtgttaaac gcgcttgacc atttgttttt aatccaatat
     4441 caaatgcttc tggatcacca agtagaacta aactcgtttt cgctctagtt aaaccagtat
     4501 ataatatcgg tctttgtaac attctaaaat actgtttaac aataggcatg ataacaatag
     4561 gaaattctga accttgtgat ttatggattg atgtacaata agcatgtgtt aattccatca
     4621 tatcttgttt cgtaaatgta atttcattac cttcaaaatc cacaacaagt acatctttat
     4681 taagggcatt ttctttcgcc caaaaaatac caacaataac tcctatgtca ccattgaata
     4741 tgttatcatt tggcctatta acaagttgta atactttgtc accttttcta aagactacat
     4801 caccaaactc aatttctcgt gtgtctttct ttttagggtt taaaatatct tgtaaaactt
     4861 gatttaaacg tttaataccg gcatttcctt tatacattgg tgcaagcact tgaatatcag
     4921 ccatagtata ccctttatta acagcactag taactacctt ctctacaact gttggtattt
     4981 ggtttgcctg acagttaata aaacttctat catgaaaacg ctgtgtaata tcaattttct
     5041 gacccaactt cattcgatgt gctaattcta taatgcttga accatcttgt tgacgatata
     5101 cttcagtcag attaactcgt ggtatagctt tcgattcaat taaatcttta aatacttgac
     5161 caggacctac agaaggcaat tggtcctcat cacctacaaa tatcaattgt gcatctaaag
     5221 gaactgcact taaaaattgg tggaacaacc aagtatctac catagacatc tcatcaatga
     5281 ttatgagtcg tgcgtttatt tcattttcta atatatcctc tggctttgtg tcttgattcc
     5341 aacctattaa acgatgaatc gtcatagctt ctaatccagt tgactcttgt agtctcttgg
     5401 acgctcttcc tgttggcgct gctaatacaa ctggataatc atcattgaca taatcatcat
     5461 aatctaatga taagccatga atctcagcat ataattcaac aataccttta attactgtcg
     5521 tttttcctgt tcccggtcca ccggttaata gcatcacctt agaattgata gccgtttgca
     5581 aagcttcttt ttgtgaagct gcatagttca cttgattcgc atcttctatt tcaccaatat
     5641 gcatttgtaa atctgactgt tcaatttctg taagtttatt tgtatgcgtc tttattctga
     5701 ataagttttg aacacttttg atttcagaat aatacaaact tggaattgca acttgttcat
     5761 tgtcaataat tagtcgtttt tcctcattta agtattgcaa catttcgtct aatttttcag
     5821 gttcgatgac ctcttcatct tgataattta atacatcaac cgttaaatct ataacaacat
     5881 tgataggcaa atatgtatgt ccctgtttaa tacattcttc ttctaacgta tagagcaacg
     5941 cagcttttaa tcgttcatta tcgttataag cgataccaat atttctagca agttgatctg
     6001 ctttattaaa accaatacct ttaatatcat aaatcaattg atatggattt cgatctaaaa
     6061 tagtcagtgt atcgccgaga taaaactgat aaattgccat tgaaagttta ggaccaaacc
     6121 ctaaatcatg taaacgaatc attatttttt cagattcttg atttgctgaa atttgttctg
     6181 caatttgttt ctgtttcttt ttagataatc ccgaaacttt ttctagcact gaatggtcat
     6241 ctaatatatc atttatcgca ttgtcaccta atgtattaac aatattttga gctgtctttt
     6301 tacctacacc tttaaacaaa tcactagata aataacttat aattgcttct ttcgtttgtg
     6361 gcatttcttt ttcaaaagtc tctgctttta attgtttacc ataacgtgga tgatcaacaa
     6421 cttgcccttt aaatgtgtag acatcgcctt caacaatatt cggaagaaac cctacaacag
     6481 ttggcattgt atcaaagtct tcatttgttt caatagtatc tactttaagc actgtataaa
     6541 aattatcact gttttgaaac aatatcgctt caacagtacc tttgatcatt gaataatcaa
     6601 atagtgtagg gtctgacatg ttactcctcc tctttcattt tagtgaatgt tttcagcgca
     6661 tgctgactta ataagtgttt agggtcgata gtcacagctt ctttaaaatg agttattgct
     6721 tcatcaatat cttcattttt cataaataac gctaagccca aattgtatct tgcatcaaca
     6781 tgatttttat caatcgttaa tacatgttta agttgagtta tggcttcatt aaacatttct
     6841 aattgacata atacaagacc atattgaaat tgaacttctg catctttgtc tttatctagt
     6901 tccgcagcag tcattaaata cggcaatgcc agcttaaatg attctaactg attaaacgcc
     6961 ataccgatca tataattaca atcaacttgt tcaatctctg tttgtaatgc ttgttgatat
     7021 aatttaatag cttcttgata acgttgctga ttataatata catttgctag attataaaat
     7081 acgacgccat tcttcggatc tattgttaaa gctttttgga aaaagcgctc tgccttttca
     7141 atctcattcg catcagcaag tacgatacca gcattaatat aattttcaat aattgtagga
     7201 ttttcttcga tatttccgaa caatgcttgt aacgcttctt ctatttttcc attttgtatg
     7261 tattgataaa ttgtttgttg atctatcatt tacgaacctc atttctcatc aattataaca
     7321 tcttgataaa ttgtatgtct cgaatcactt aacaacgaat aaaatataat ctaatatcat
     7381 cttcattcat gaaaaagcgg gaatggaata gaaatgatta agaaccatta acggtttatt
     7441 atgtaatggt tcttccacat tagccactac tattatgtac ttaaaaatag gaatacataa
     7501 ttagactcat gcatagggag tgggacagaa atgatatttt aacaaaatta aattcgttta
     7561 tccccaactc gcattgcctg tagaatttct ttacgaaatt ctctgtgttg gggccccatc
     7621 cccaacttgc acattattga aatctgactt ttggtcagct tctgtgttgg ggccccgtcc
     7681 ccaacttcca ttgcctgttg aaattggggt cccaatttct ctgtgttggg gccccgccaa
     7741 tataacattg tagagcctag gacattgtga tgtcccagac tctatcatca tgaattattc
     7801 tcatcaaaaa ctgtctttcg tcattttcga cgttgaaact tcaaataagt aatttattgt
     7861 tgccattgtt tatacaacat aatttaattg accttcattt ttgaacacat cgtcaattgt
     7921 tgctccacca agacacacat caccttgata aaaaacaact gcttgtccag gtgtgattgc
     7981 tcttactggc tcagcaaaag taacacgtag tgcatggtcg ttttcacgtt tcacaaaaac
     8041 tttcgtatct ttttggcgat atctaaattt agctgtacat tcaaaacctt gatctaagtc
     8101 attatcttct ggatttacaa atgaatagtc tgaagcaatt aagtaatcac tgtataatgc
     8161 atcgtgatgg aatccttgtt ctacatataa aacattatct tttagatttt taccgacaac
     8221 aaaccaagga tcgccatctc cacctatacc taaaccatgt ctttgtccta ttgtgtaata
     8281 catcaaccca ctatgtttac ccattttctt accatcaagt gttatcatat cacccggttg
     8341 cgcaggtaga tattgagata aaaatgtttt aaagtttttt tcgccgataa aacaaatacc
     8401 tgtagaatct tttttcttag cagtaactag tccttgttct tcagcaattc gacgcacttc
     8461 aatcttttcg atgtcgccaa ttgggaacat cacttttgaa agttgttgtt gggataattg
     8521 attcaagaag tatgtttgat ctttattatt atctacacca cgtaacattt caacatgacc
     8581 atcttcatga cgatgtatgc gtgcgtaatg ccctgttgct acataatctg cacctaaatt
     8641 catcgcatga tctaaaaagg ctttaaactt aatttcttta ttacacataa cgtctgggtt
     8701 tggagtacga ccttttttat attcatctaa gaaatacgta aagactttat cccaatattc
     8761 tttttcaaaa ttaacagcgt aatatggaat gccaatttga ttacacactt caataacatc
     8821 gttgtaatct tcagttgcag tacatacgcc attttcgtca gtgtcatccc agtttttcat
     8881 aaatatgcca atgacatcat aaccttgttc ttttaagacg tgggctgtta cagaactatc
     8941 tacaccgcct gacataccaa cgacaacacg tatatcttta tttgacaatt atgactcctc
     9001 cttaaattta aaatatattt tatgaatttc agctacaatt gcattaattt cattttcagt
     9061 agtcaattcg ttaaaactaa atcgaatcga atgatttgat cgctcttcat cttcgaacat
     9121 tgcatctaaa acatgcgacg gttgtgtaga gcctgctgta catgcagatc cagacgacac
     9181 atagatttgt gccatatcca acaatgttaa catcgtttca acttcaacaa acggaaaata
     9241 tagatttaca atatggcctg tagcatccgt cattgaacca tttaattcaa atggaatcgc
     9301 tctttcttgt aatttaacta aaaattgttc ttttaaattc attaaatgaa tattgttatc
     9361 gtctcgattc ttttctgcta attgtaatgc tttagccatc ccgacaattt gtgcaagatt
     9421 ttcagtacct gcacggcgtt tcaattcttg ttcaccgcca agttgaggat aatctaatgt
     9481 gacatggtct ttaactaata atgcaccgac accttttggt cctccaaact tatgagcagt
     9541 aatactcatt gcgtcgatct caaattcgtc aaacttaaca tcaagatgtc caattgcttg
     9601 aaccgcatca acatggaaat atgcatttgt ctcagcaata atatcttgaa tatcataaat
     9661 ttgttgcact gtgccaactt cattatttac aaacataata gatactaaaa tcgtcttatc
     9721 tgtaattgtt tcttcaagtt ggtctaaatc aatagcacct gtatcatcaa catctagata
     9781 tgttacatca aaaccttctc gctctaattg ttcaaaaaca tgtaacacag aatgatgttc
     9841 aatcttcgat gtgataatgt gattacccaa ttgttcattt gcttttacta tgcctttaat
     9901 tgccgtatta ttcgattctg ttgcaccact cgtaaatata atttcatgtg tatctgcacc
     9961 aagtaattgt gcaatttgac gtcttgactc atctaaatat ttacgcgcat ctcttccctt
    10021 agcatgtatt gatgatggat taccataatg cgaattgtaa atcatcatca tcgcatctac
    10081 tacttcaggt tttactggtg tggtcgcagc ataatctgca taaatttcca tgtttggaca
    10141 ctcctcacaa ttttatcaat gttccaataa tagcacctta catactattt ttctactttt
    10201 ctgtttaact ttatttataa tgtttttaat tatattttac cattttctac acatgctttt
    10261 cgataggctt ttttaagttt atcgctttat tcttgtcttt tttataaatt ttagtatttg
    10321 cagatatttt ttcattcgta aaatgtaacg tactattatt ttggttatga gcaatttaat
    10381 atttatctgg ttattcgatt ggtatacttc ttatatcata caaaaggaag gacgatacaa
    10441 aaatggcgat taaatattca gcattaaacc ttgtccctat tcgagaaggt gaagatgaac
    10501 gaacagcaat taatgatatg gttaagctcg cacaacattt agacgaatta tcatatgaaa
    10561 gatattggat tgctgaacac cataacgctc ccaacctagt aagttcagcg actgctttat
    10621 tgattcaaca tacgttagaa catacgaaac acatacgtgt aggttctgga ggaatcatgt
    10681 tacctaacca tgctccatta atcgttgcgg aacaatttgg cacgatggca acattatttc
    10741 caaatcgtgt cgatttagga ttaggacgtg cacctggaac agatatgatg accgcaagtg
    10801 cattaagacg agatcaacat gatggtgttt ataaatttcc ggaagaagtt tcattattac
    10861 aacaatattt cggtcccgct caccaacaag catatgttcg tgcttatcca gcagtaggta
    10921 aaaatgtgcc tttatatatt cttggttctt caacagattc tgcacattta gctgctcgta
    10981 aagggcttcc atatgtgttc gctggacatt ttgcacctca acaaatgaaa gaagctatcg
    11041 aaatttacaa aacgttattt gaaccttctg atgtattaga cgaaccttat gttattgtat
    11101 gtttaaatac aatcgttgct gaaaatgatg acgaagcaca atatttagct tcatctatgg
    11161 cacaagtaat ggttagtatc actcgtggca gaatgcagcc cgttcaaccg ccaacacatg
    11221 aactacaaaa tatattaacg ccgagagaat atgcgatggc tatggaaaga cagaaaatat
    11281 cattaatagg ttcagaaaat actgttcaac aaaaaattca agattttatg gaaacttatg
    11341 gtgaagtcaa cgaaattatg gcaataagtt atatttatga taaagatatg caattagact
    11401 cttatcgtcg gttcaagaat gttataaatc agataaatga aaaaaacact ttataatgtg
    11461 ataaataaac taagtgaaag tatgtatcca taatattaat aaaaataaac agtaacagca
    11521 ttttgaataa aagatgtctt tattgttcaa tcatttattt tagtaatgat tcaaattcac
    11581 ttaaaat
//
LOCUS       DBNENW010000080        11576 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNENW010000080 DBNENW010000000
VERSION     DBNENW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.080"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(411..1340)
                     /locus_tag="AC1LW8_001699"
     CDS             complement(411..1340)
                     /locus_tag="AC1LW8_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIP4773529.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1535..1759)
                     /locus_tag="AC1LW8_001700"
     CDS             complement(1535..1759)
                     /locus_tag="AC1LW8_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HIP4773530.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1776..1979)
                     /gene="sroA"
                     /locus_tag="AC1LW8_001701"
     CDS             complement(1776..1979)
                     /gene="sroA"
                     /locus_tag="AC1LW8_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigS mRNA-stabilizing protein SroA"
                     /protein_id="HIP4773531.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
     gene            2138..2698
                     /locus_tag="AC1LW8_001702"
     CDS             2138..2698
                     /locus_tag="AC1LW8_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP4773532.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            2717..5119
                     /gene="mnhA2"
                     /locus_tag="AC1LW8_001703"
     CDS             2717..5119
                     /gene="mnhA2"
                     /locus_tag="AC1LW8_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HIP4773533.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            5106..5531
                     /gene="mnhB2"
                     /locus_tag="AC1LW8_001704"
     CDS             5106..5531
                     /gene="mnhB2"
                     /locus_tag="AC1LW8_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HIP4773534.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5528..5872
                     /gene="mnhC2"
                     /locus_tag="AC1LW8_001705"
     CDS             5528..5872
                     /gene="mnhC2"
                     /locus_tag="AC1LW8_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HIP4773535.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5862..7358
                     /gene="mnhD2"
                     /locus_tag="AC1LW8_001706"
     CDS             5862..7358
                     /gene="mnhD2"
                     /locus_tag="AC1LW8_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HIP4773536.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            7359..7841
                     /gene="mnhE2"
                     /locus_tag="AC1LW8_001707"
     CDS             7359..7841
                     /gene="mnhE2"
                     /locus_tag="AC1LW8_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HIP4773537.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            7838..8140
                     /gene="mnhF2"
                     /locus_tag="AC1LW8_001708"
     CDS             7838..8140
                     /gene="mnhF2"
                     /locus_tag="AC1LW8_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HIP4773538.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            8115..8552
                     /gene="mnhG2"
                     /locus_tag="AC1LW8_001709"
     CDS             8115..8552
                     /gene="mnhG2"
                     /locus_tag="AC1LW8_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HIP4773539.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            8892..10934
                     /locus_tag="AC1LW8_001710"
     CDS             8892..10934
                     /locus_tag="AC1LW8_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIP4773540.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         3843 a         1585 c         2024 g         4124 t
ORIGIN      
        1 aatctatagc acgttaaagt gtatatacgt tattccgatt gataacgggt aacaaataat
       61 agaaagtacg ataattgatc atgcattaaa gcacttttat ttttcaaact cgactcaatg
      121 aattataaaa agttgtgcat tttgacttat ttttaattaa ttgaataacc aactatgaaa
      181 tattagcacg aataacataa tttttgctat taatttggtg taaattatta aaaaataaca
      241 ttaatttagg tcttaagaat gagttgacta tcgaatttgc taaattaaaa tttgttgtgt
      301 caaagtatac tacccaaatc aacgcttttc taaagatttt tagaccacaa aaaaatagct
      361 tatcactttt aaaaataatt acaagttaaa atgataagct aaaagttaat ttaaaataat
      421 cttaaaagta taatgcctgt caaaatacat aatagaccaa tagtttttct ggatgtcatt
      481 gctattttag gtgaaccaaa taatccaaag tgatctatca atatgcccat tagaatctgg
      541 ccaaacatcc caataagtgt tgttaatgct gcacccatat gaggcattaa gataatgtta
      601 gctgttacaa aagccatacc aagtataccg ccagtaaaat agataggctt taatttaccg
      661 aattttaaat gacttgtttt tagttttaaa gaacgattaa aaatagcggt taaaatcaat
      721 agcgttattg acccaattgt aaatgatact aatgatgcaa aggctggtga atgagtatga
      781 cttgctaaag cactattaat tgtcgtttga ataggtggaa agaaaccaaa aataaatcct
      841 aagagaagcc aaaacagtaa atacttttga tcagttagta ataaattatt cttgttaaat
      901 tgattcatta tgacgatgcc gacaatgagt aacaatactc caattgcttt aattaaatta
      961 aaatcatgaa ttgtagcgcc aaataagcca aatgtatcaa taatgacacc cataataatt
     1021 tgacccgcaa ctgttgcaat tacagttaat gttgcaccta attttggcaa taacaataaa
     1081 ttgccagtta aaaagctaac cccaagcaaa ccaccgacta cccatgtgta gttaaatgat
     1141 tgattattgt aaaagtgaat agtaaatact tctggattga taatgatatt taaaataatt
     1201 aaacaaattg ttccaactga aaatgaaatg aatgaagtat agaaaggcga tttagtatac
     1261 agtgatagtc ttgaattgac agatgtttgg ataggaataa gcattccaac taggacacca
     1321 atgatataga aaagaagcat atgaataaag tctcactttc caaagttaat aataaaatca
     1381 tatgaataca ttatcattat agagaaaata tacaactcat ttacaataat gttgaaaaaa
     1441 acaggacatt atcacaacaa aatcacccta gacaatcatg atgaaagtct agggtgttag
     1501 tatttatact aaattaattg cgctttcttt atatctattt cttatcttca aataaaacag
     1561 ttgttgtttt atcaataatt tgagcagaat gtactttggt gatagtacca ctatttgact
     1621 ttataatatt taaatctact aatgcattca tactatcacg tgcaacttgc tcggtcacta
     1681 cgttgtttaa ttgtggtaat tgcagtttta caggcttgtc tagtgaagat ttaaaactta
     1741 attcaagtgt tttagtcatg aatatgtcct cctgattaaa ttgataaaga tttgatgagt
     1801 tcgatattgt tatatgtttc tccagtaagt cgctcaataa gtttgctgaa tgttttaatt
     1861 tggtcgtttg atgcatcagg gttaatgtta gcgaatcgac gcttaaattc tgtttgtttg
     1921 ccgttagcgt ctactttagt aaatgataat acaatagtga tgtggtttat tttactcata
     1981 ttttaaaacc tcctttcaca ctatatatcg aaataaaata ataaaatggc taattttatt
     2041 ttctatgttt aaaatctata aaaaaggcaa tagatatatg taactaaaat ataaaatagc
     2101 tatgattaag acacttaaag caaggggagg ttgagtaatg aataaagtag aagcgattaa
     2161 atttaatgat gatattgtta aaatgtatga agcgctcaag ataaaatctg aacgtgacta
     2221 tttattcttt aagttagcta tacatagtgg attgaaagta tcagaattat taacaattac
     2281 agtctctcaa gttaagagac taattgaaaa gtgtacgtta tcagaaatgt gtaaagcaca
     2341 ttttcattcg ttgattaaaa ttaggttacc agaaacatta tcgaaagaac tacttcaata
     2401 tatagaggac aggagtcttt cgaatgaaga cgttcttttt caatcactac gaacaaatca
     2461 agtattatct agacagcaag catatcgaat aattcaccaa gcatcaattg aagctggtat
     2521 agataatgta ggactaacga cattgcgtaa gacatttgca tatcatgctt atcaaaaagg
     2581 tatacctata ccagtcattc aaaagtattt agggcatcaa tctgctattg aaacactaaa
     2641 ttttatcggt ttagaaaatg agtgtgaaca tagtatttat atttcattac aattatagaa
     2701 acaaaggagg ctaataatga gtttggttta tttattaatt gctatacttg tgattatggc
     2761 gatgatactt ctaatgtcta aacgtagagc attggctaaa tatgccgggt acatagcgtt
     2821 ggttgcacct gtaatttcat ctatctattt tttgattcaa ataccatcag tagctaaact
     2881 gcaatatctt tctacctcta ttccatggat taagacatta gatattaatt tagatttacg
     2941 tttagatggt ttaagtttaa tgttttctct tattatttca cttattggaa ttgcagtatt
     3001 cttctatgca actcaatatt tatcctctcg aaaagacaat ttaccaaggt tttattttta
     3061 tttaacgtta tttatgttca gtatgattgg tattgtatta tcagacaata cgatattgat
     3121 gtacattttt tgggaattaa cgagtgtatc atcattttta ttgatttcat attggtataa
     3181 caacggagat agtcaatttg gtgcgatgca atcatttatg attacagtat ttggtggttt
     3241 ggcattatta gttggtttta tcatgttgta tattatgaca ggaacgaata acatcacaga
     3301 gatattagga caagcagatc atattaagaa tcatggattg tttatcccta tgatttttat
     3361 gtttttatta ggtgcattta caaaatcagc acaatttcca tttcatattt ggctacctag
     3421 agcaatggct gcacctacac ctgtaagtgc ttatttacat tcagccacga tggtaaaagc
     3481 tggtatcttt ttattacttc gatttacacc attattaggt cttagcaata tgtacgtata
     3541 tatcgttacg tttgttggtt taataacaat gttatttggt tcaattacag ctttaaaaca
     3601 atgggattta aaaggtatcc tagcgtactc tacaatcagt caacttggga tgattatggc
     3661 tatggtgggt ataggtggcg gatatgctca acaccaacaa gacgcaatag catctattta
     3721 tgtatttgta ttatttggtg cgctatttca tctaatgaat catgccatct ttaaatgtgc
     3781 gcttttcatg ggagtaggta ttttagatca tgaagcaggt tcaagggata tacgaatttt
     3841 aagtggaatg cgtcaactat ttcctaaaat gaatctagtc atgacgatag cggctctatc
     3901 tatggctgga gtaccatttt taaatggatt tttaagtaaa gaaatgtttt tagatgcatt
     3961 aacacaaact ggacaattat cccaatttag tttgatttca atgatagcta tcgtgtttgt
     4021 tggtgttatt gcgagtgttt ttacattcac atatgcacta tacatggtaa aagaagtatt
     4081 ttggacaaaa tatgattcta aggtttttac taaaaaaaat atccacgaac catggttgtt
     4141 tagtttacca tctcttatat taatggtgct agtacctgta atcttttttg taccaaatat
     4201 atttgggaag gggattatcg ttctagcatt aagagctgta tcaggtggta atcatcaaat
     4261 tgatcaattg gcaccacatg tttcgcaatg gcatggattt aacataccgc ttcttttaac
     4321 catcatcatt attttattgg gtagtgtact agcaatcaaa gtagattgga aaaaagtgtt
     4381 cacaggtaaa attagacaga tttcagtttc aaaaagctat gagatggtat atcgacattt
     4441 tgaaaagttt gctacgaagc gatttaaacg tgttatgcaa gatcgtttaa accaatacat
     4501 tattatgacc ttaggcatat ttatgattat cattggatat ggttatattc gaattggact
     4561 tcctaaagta catcagttac atgtttctga atttggggca ttagaaatta tattagcaat
     4621 cgtaactgtc acaattggta tttctttaat ttttatacgt caacgactga caatggtcat
     4681 tttaaatgga gtcatcggat ttgttgtgac cttattcttt atagcaatga aagcccctga
     4741 tctagcattg actcagctag tagttgaaac aataacgacg atactattta ttgtcagttt
     4801 ttcaagatta ccaaacgtgc caagatctaa cgctaacaaa aaaagagaaa taattaaaat
     4861 ttctgtatca ctcttgatgg cacttattgt tgtatcatta atttttatta cacaacaaac
     4921 agatggttta tcatcaatat cagactttta tttaaaagct gacaaactaa caggtggtaa
     4981 aaatattgta aatgcgatac ttggtgactt tagagcatta gatacattat ttgaaggatt
     5041 agtgttaatt attactgggc taggtattta cacattatta aattatcaag atcggagggg
     5101 acaagatgaa agagaatgat gtcgtgttaa gaacggtcac gaaacttgtt gtatttattt
     5161 tattgacttt cggattctat gtcttcttcg caggtcataa taatcctggt ggtgggttta
     5221 ttggtggttt aatatttagt tcagcgttta ttttaatgtt tctggctttt aatgttgaag
     5281 aggttttaga aagtttaccg attgatttta gaattttaat gattattgga gcattggtat
     5341 catctattac tgcgataata cctatgtttt ttggaaaacc atttttgtct caatatgaaa
     5401 caacttggat acttccaatt ttaggacaaa ttcatgtaag tacaataaca ctttttgaat
     5461 taggtatttt attctcagtt gttggtgtta ttgtcacagt gatgttgtcg cttagcggag
     5521 gtcgatcatg aatttaatat tattactagt tataggattt ttagtgttta taggaacata
     5581 tatgatttta tcaatcaatt taattcgtat tgtaatcgga atttcaatat atactcatgc
     5641 tggtaatctc attattatga gtatgggaac gtatggttct agtagatcag aaccactaat
     5701 aactggtgga aaccaattgt ttgttgatcc cttgttacaa gctattgtac taactgcaat
     5761 agttataggg tttgggatga ctgcgttttt acttgtactt gtttatagaa cttataaagt
     5821 aacaaaagaa gatgaaattg aaggcctaag gggggaagat gatgctaagt aacttattga
     5881 ttttaccaat gttattacca ttcctttgtg ccttaatcct tgtattttta aaaaataatg
     5941 atcgtatttc taaatattta tacttaggta caatgactat caccacaatt atttcattaa
     6001 tgctattaat ttatgttcag cgtcaccgtc caattacgct agactttgga ggatggtcag
     6061 cgccctttgg tatacagttt ttaggagatt ctttaagttt aattatggtt acaaccgctt
     6121 cgtttgtgat tactttaatt atggcatacg gatttgggcg tggcgaacat aaagcaaatc
     6181 gttatcactt gccatcgttc atattatttt taagtgttgg cgtgataggc tcttttctaa
     6241 catcagattt atttaattta tacgtcatgt ttgaaattat gttactagcg tcatttgtac
     6301 tcattacact tggacaatct gtagaacaat tacgtgctgc aattatttat gttgtcttga
     6361 atattattgg ttcatggcta ttcttattag gtataggttt actttataaa acagtaggta
     6421 cattaaactt ttcacatatt gcaatgcgtt tgaatgacat gggagataat cgcactgtta
     6481 caatgatttc attaatcttc ttagtcgcat ttagtgcgaa agcagcgctg gtccttttta
     6541 tgtggctacc caaagcctac gctgtgttaa atactgagct tgcagcatta tttgcagcgt
     6601 taatgaccaa agtaggggcc tatgcattaa ttcgattctt cactttacta tttgatcaac
     6661 ataatgatct catacatcca ttgctagcaa ctatggctgc tataactatg gtcatcggcg
     6721 ctataggtgt cattgcttat aaagatatta aaaagattgc agcttaccaa gtcataatct
     6781 caataggatt tatcatttta ggtttaggaa caaacacgtt tgcaggtatt aatggtgcaa
     6841 tattttattt ggtaaatgac attgttgtaa aaacattgct attttttatt attggtagtt
     6901 tagtttacat tacaggctat cgacaatatc aatatttgaa tggcttagct aaaaaagaac
     6961 ctttatttgg agttgcgttt attataatga tttttgctat tggcggcgtg cctccattta
     7021 gtggctttcc ggggaaagta cttattttcc aaggtgcatt gcaaaatggc aattatattg
     7081 gactagcgtt aatgattatt actagtctaa ttgcaatgta cagtttattt aggatacttt
     7141 tttatatgta ttttggagat aaagatgggg aggaagttaa ttttaagaaa atcccgctat
     7201 atcgaaaaag aattttaagt attttagtag ttgtggttat cgcaatcgga attgctgcac
     7261 ctgttgtgtt aaatgttaca agtgatgcaa ctgagttgaa cacgagtgat caattatatc
     7321 aaaaacttgt aaatccgcat ttgaaaggag aggactaaat gaatcaaata gttttaaata
     7381 ttatcattgc attcttatgg gtattatttc aagatgaaga tcattttaaa ttctcgactt
     7441 tcttttctgg atatctaatt ggtttaattg tcatttatat attacacagg tttttcagcg
     7501 atgattttta tgttagaaaa atatgggtag ctattaaatt tttaggtgtt tatttatatc
     7561 aattaataac atctagcatt agcacgatta attatattct ttttaaaaca aaagatatga
     7621 accctggatt actttcatat gaaacaagac taacaagtga ttggtcaata acatttttaa
     7681 caattttaat tattataact ccagggtcta cagtaatacg aatttctcaa gactctaaaa
     7741 agttttttat tcatagtatc gacgtgtcag aaaaagaaaa agatagtttg ttaagaagta
     7801 ttaagcatta tgaagactta atattggagg tgtcgcgatg atacaaacaa taacacatat
     7861 tatgattatt agttcactca ttatttttgg aattgcatta atcatctgtt tatttagatt
     7921 aatcaaggga cctacaacag cagatcgtgt cgttacattt gatacaacaa gtgctgtcgt
     7981 aatgtcaatt gtgggtgtgt taagtgtact tatgggcacc gtttctttct tagattcaat
     8041 catgctcatt gccattatat cttttgtaag ttctgtttca atatcacgct ttattggtgg
     8101 ggggcatgtg tttaatggaa ataacaaaag aaatctttag tcttattgct gctgtgatgt
     8161 tgttgttagg tagttttatt gctcttatta gtgcaatagg tatcgtgaaa ttccaagatg
     8221 ttttcttaag aagtcacgct gcgacaaaaa gttcaacttt atccgtgtta ttaactttaa
     8281 tcggtgtatt aatttatttt attgtgaata caggattttt cagtgtgcgt ttattactgt
     8341 cacttgtttt tattaattta acttcaccag tcggcatgca cttagtcgct cgcgctgctt
     8401 atcgcaacgg cgcttatatg tatcgaaaaa atgatgctca cacacatgca tcaatattat
     8461 taagttcaaa tgaacaaaac tctacagaag cattacaatt acgtgctgaa aaacgagaag
     8521 agcatcgtaa gaaatggtat caaaacgatt gaagtgttgg ctaaattgtt aagcataaca
     8581 atgcttttga aaatctgttt tcaaaattaa tcattaaaag agtactttga aattggttag
     8641 tcgattttaa ggtgctcatt ttttgtatta taagttaatg ttgttatgtg aaagcaagtt
     8701 gtttatttat acaaacaatt agtgataaaa taaaattaaa taattgcaaa tttaagtact
     8761 gcaatcgtag acatatatct atattaaagt gcttttgtgg tcgctaactc aatttatcct
     8821 ctaaagtata attacaacga gatgtgatac ttaattttca attaaacaat actttttaga
     8881 gaggtgaaag tttggaaata tttgaaacaa ttcttatatt tatagctgtt gtgatactaa
     8941 gttcgtttgt ccatactttc atacctaaag tacccctagc atttatacaa attttcttgg
     9001 gcatgttact atttattacc ccaatccctg ttcaatttaa ttttgattct gaattgttta
     9061 tggtaacaat gattgcgcct ttgttatttg tagaaggtgt taatgtttct agagtccatt
     9121 taaggaaata tattaagcca gtgatgatga tggcattagg attagtcatt actactgtga
     9181 taggtgtagg tttatttatt cattggattt ggccagattt acctattgga gcagcatttg
     9241 caattgctgc cattctttgt cctactgatg cagtagcagt gcaagcaatc actaaaggaa
     9301 aggtcttgcc aaaaggagca atgacaattc ttgaaggtga gtcattattg aatgatgctg
     9361 ctggtattat ttcatttaaa atagctgttg gagtattagt tacaggtgct ttttcacttg
     9421 ttgatgctgt tcagttgttt ttaattgcat caattggtgg cgcagtggtt ggtttactta
     9481 taggtatggc attagtaagg ttccgattaa cattgatgcg tcgaggatat gaaaacatta
     9541 atatgtttac aattattcaa ttgttaacac catttgttac gtatttaatt gctgaattgt
     9601 ttcacgcatc aggaatcatt gcagcagtag ttgcaggact tgtacatggt ttcgaacgtg
     9661 acagaattat gcaagtacgt acacaactgc aaatgagtta caatcataca tggaatatac
     9721 taggttatgt tttaaatggc tttgtttttt caatattagg atttttagta cctgaagtta
     9781 ttattaaaat tatcaaaaca gaaccgcaca atttaatctt tttaataggc atcactattg
     9841 ttgttgcttt agctgtctat ctatttagat ttgtttgggt ttatgtctta tatccttatt
     9901 tttatttagc catcagtcca ttccaaaaaa tgatgactaa aaatgatgat gataatccaa
     9961 cgactgagaa accaccaaag cgaagtttat acgctttaat tatgacgtta tgtggtgtgc
    10021 atggaacaat ttctttagca atcgcattaa cgttaccgta ttttttagca gggcatcatg
    10081 cttttacgta tagaaacgac ttattattta ttgcatctgg tatggttatt attagtttgg
    10141 tagttgcgca agtattattg ccattattaa cgaaacctgc acctaaaaca gtaattggca
    10201 atatgtcgtt taaagttgct agaatttata tattagaaca agttattgat tatctaaatc
    10261 aaaaatctac tttcgaaaca agttttaaat atggtaacgt gattaaagaa tatcatgata
    10321 aattagcatt tttaaaaact gtagagaaag atgatgaaaa ctctaaagaa ttagaacgtc
    10381 tacaaaaaat tgcttttaat gtagaaacaa aaacattaga gtctttagta gatgaaggac
    10441 aaataacgaa tagtgtactt gaaaactata tgcgttatgc tgaaagaaca caggtatata
    10501 gacaagcatc attaataaga agaatgattg tattattacg aggtgcttta ttaaaacgaa
    10561 gagtacaaac gagagtgaac tccgcatctt cacttagtgt tacggataac ttaatggaat
    10621 taaataaaat taataaatta gtccattata atgtggttag tcgtttgtct aaggaaacaa
    10681 caaaagataa tacacttgaa gttggaatgg tttgtgacgg ttatttaatg cgaattgaaa
    10741 acttaacacc atcaaatttc ttcaactcag caagtgaaga tacgattact aaaattaaat
    10801 taaatgcatt gagagaacaa cgtcgcattt tacgtgagtt gattgataca gatgaagtat
    10861 cagaaggtac agcgttaaaa ctaagagaag ccatcaatta cgatgaaatg gttattgtag
    10921 atagtatgac gtagttccta attatgctaa aagggattga tgaaaaactg aagggctttt
    10981 catcaatcct ttttatttta tgggaattga atagatagtt ttaaactata cgaattatta
    11041 atatttgaga tttaattgaa ataagtttta aaaattggag gagatagatt aagcgaagtc
    11101 atttaaaggt gaagttaagt gtattcacaa aaaatagcca cactcatatg acatcggatg
    11161 agtgtggctt aaggatctat ggggggagga aaccatagat gtttactttg ataggccaga
    11221 ttaaatatca aagtatgcga ttatttatag cttgatgcaa aagtggtatg cctattaaaa
    11281 gttactgcac atagctttta atattccgtt caaaggaaag gggcatacaa ttgaacaatc
    11341 tgtaatagta cttttaacca gctatgctaa aagtctagta gggagaacag ttgtccaatc
    11401 acataagaac ctctaacttc gttagtacga ttaagaaaag ctttttagtt agtatgtaat
    11461 acaatttatt gacgcgcgtg aatctctttt ataagagtgt gtagggaatg gcgttgtata
    11521 aattgtatta gaagaacttc taacgcatct ctgtggttaa aagagatgaa gggaac
//
LOCUS       DBNENW010000081        11421 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNENW010000081 DBNENW010000000
VERSION     DBNENW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.081"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            17..322
                     /locus_tag="AC1LW8_001711"
     CDS             17..322
                     /locus_tag="AC1LW8_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HIP4773541.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            312..2003
                     /locus_tag="AC1LW8_001712"
     CDS             312..2003
                     /locus_tag="AC1LW8_001712"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIP4773542.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLRENFRPLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVNMVEAGRDTLDRIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSIDLDEMKEEWEKAKRTPDERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNDIISLDELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLTHSWI
                     PKHKVEYSNEKIPYIEWEEDGLLTIQDNPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            2008..3246
                     /locus_tag="AC1LW8_001713"
     CDS             2008..3246
                     /locus_tag="AC1LW8_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIP4773543.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVSTEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSIFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3230..4003
                     /locus_tag="AC1LW8_001714"
     CDS             3230..4003
                     /locus_tag="AC1LW8_001714"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HIP4773544.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIANVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            4015..5178
                     /locus_tag="AC1LW8_001715"
     CDS             4015..5178
                     /locus_tag="AC1LW8_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIP4773545.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5247..5525
                     /locus_tag="AC1LW8_001716"
     CDS             5247..5525
                     /locus_tag="AC1LW8_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIP4773546.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5537..5869
                     /locus_tag="AC1LW8_001717"
     CDS             5537..5869
                     /locus_tag="AC1LW8_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773547.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5866..6267
                     /locus_tag="AC1LW8_001718"
     CDS             5866..6267
                     /locus_tag="AC1LW8_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773548.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDRALNKASEFFIKTLK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6268..6663
                     /locus_tag="AC1LW8_001719"
     CDS             6268..6663
                     /locus_tag="AC1LW8_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HIP4773549.1"
                     /translation="MNILNTVKGILLSDAELQTYINSRIYYYKVTENAETSKPFVVIT
                     PIYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6698..7339
                     /locus_tag="AC1LW8_001720"
     CDS             6698..7339
                     /locus_tag="AC1LW8_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HIP4773550.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDRGEHSDDSLGSLPS"
     gene            7431..7886
                     /locus_tag="AC1LW8_001721"
     CDS             7431..7886
                     /locus_tag="AC1LW8_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIP4773551.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLDADTTYPKGTYQVS
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSITVNTDDNVEPNIAPSTATNKTLK
                     YTSEHPEFVTVDERTGAIHGVAEGTSVITATSTDGSDKSGQISVTVTNG"
     gene            7944..8294
                     /gene="gpG"
                     /locus_tag="AC1LW8_001722"
     CDS             7944..8294
                     /gene="gpG"
                     /locus_tag="AC1LW8_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone G"
                     /protein_id="HIP4773552.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALKDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8336..8494
                     /locus_tag="AC1LW8_001723"
     CDS             8336..8494
                     /locus_tag="AC1LW8_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773553.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8508..>11421
                     /locus_tag="AC1LW8_001724"
     CDS             8508..>11421
                     /locus_tag="AC1LW8_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIP4773554.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAFQKLKQLREAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KSTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLAIFGASIGPAVLAGGLLIRAVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMLFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENNRIMEKVRLNSGQITEDKAKKLLKIEADLSNNLIAEIEKRNKKELE"
BASE COUNT         4473 a         1566 c         2241 g         3141 t
ORIGIN      
        1 gaaagggggt ctttatatga aattaacaaa aaaacagctg aaagaatata tagaggatta
       61 taaaaaatct gatgacatat taattaattt gtatatagaa acgtatgaat tttattgtcg
      121 gttaagagat gaacttaaaa atagtgattt gatgatagag catacaaaca aggctggtgc
      181 gagcaatatt gttaagaatc cattaagcat agaactgaca aaaacagttc aaacactaaa
      241 taacttactc aagtctatgg gtttaactgc agcacaaaga aaaaagatag ttcaagaaga
      301 aggtggattc ggtgactatt aaagttttaa atgaaccttc accaaaacta ttaacaacat
      361 ggtatgcaga gcaagtcact caagggaaaa taaaaacaag caaatatgtt aaaaaagaat
      421 gtgagagaca ccttagatat ctagaaaatg gaggtaaatg ggtatttgat gaagaattag
      481 cgcaccgtcc tattcgattc atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc
      541 aacttgtatt acaaccatgg caacatttta ttattggcag tttgtttggt tgggttcata
      601 aagaaacaaa actgcgcagg tttaaagaag ctttgatatt tatggggcga aaaaatggta
      661 aaacaactac tatatctggt gttgctaact atgctgtttc tcaagatgga gaaaacggcg
      721 ctgaaatcca tcttttagca aacgtaatga aacaagctag gattctattt gatgaatcta
      781 aggcgatgat taaagctagc ccaaagctta gagaaaattt tagacctttg agagatgaaa
      841 ttcattacga tgcaactata tctaaaatta tgccacaggc ttcagacagt gataagttgg
      901 atggtttaaa tacacatatg ggcatttttg atgaaattca tgaatttaaa gattataaat
      961 tgatttcagt tataaaaaac tcaagagcgg caaggttaca accccttctt atctacatta
     1021 cgacagcagg gtaccaacta gatggaccac ttgttaatat ggtagaagcg ggaagagaca
     1081 ccttagatcg aatcatcgaa gatgaaagaa ctttttacta tttagcttct ctcgatgatg
     1141 acgatgatat aaatgattcg tcgaattgga ttaaagcaaa tcctaaccta ggtgtttcta
     1201 tcgatttaga tgaaatgaaa gaagagtggg aaaaggctaa gagaacacca gacgaacgtg
     1261 gagattttat aaccaaaagg tttaacatct ttgctaataa tgatgagatg agttttattg
     1321 attatccaac acttcaaaaa aataatgaca ttatttcctt agatgagttg gaaggtagac
     1381 catgtactat aggttatgat ttatcagaaa cagaggactt tacagccgca tgtgccactt
     1441 ttgcattaga taatggcaaa gttgctgtct taacacattc ttggattcct aagcataaag
     1501 ttgaatattc taacgaaaag ataccctata tagaatggga agaagacgga ttactaacaa
     1561 tacaagataa tccttatata gactaccaag atgttttaaa ttggataata aagatgaatg
     1621 agcattatgt tgtcgaaaaa atcacttatg atagggcgaa tgcttttaaa ttaaatcaag
     1681 agttaaagaa ttatggcttt gaaacagaag aaacaagaca aggggctttg accttgagcc
     1741 ctgcattgaa ggatctaaaa gaaatgtttt tagatgggaa aataatattt aataataatc
     1801 ctttaatgaa atggtatatc aataatgttc agctgaaact agacagaaat gggaactggc
     1861 tgccatctaa acaaagcaga tatcgtaaaa tagatggttt tgcagcattt ttaaacacat
     1921 atacagatat tatgaataaa gttgtttctg acaagggtga aggaaacata gaatttatta
     1981 gtattaaaga tataatgcgt taaggaggtg aatgttatcg caaaagagaa tattgtcaca
     2041 cgcataaaga aaaaattgat agacaattgg attgatcagt cagcttctaa gctttatgac
     2101 tttagcccat ggaaaaataa atctttttgg ggtgtaatca ataatacgct tgaaactaat
     2161 gaaacgatat tttcagctat tacaaagtta tctaattcga tggctagttt gcccttgaaa
     2221 atgtatgaag attataaagt agttaataca gaagtatctg atttacttac agtgtcaccg
     2281 aataattctc tgagcagttt tgattttatt aatcaaattg aaacaatcag aaatgaaaaa
     2341 ggtaatgcat atgtgctaat tgaacgagac atctatcatc aaccatcaaa gcttttctta
     2401 ttaaatccag atgttgttga aatgttaatt gaaaaccaat cacgtgaact ttattattcc
     2461 attcatgctg caactggaaa taaattgatt gttcataata tggacatgtt gcattttaaa
     2521 cacatcgtgg catctaatat ggttcaaggt attagtccga ttgatgtgtt gaagaataca
     2581 actgattttg acaatgcagt aagaaccttt aatcttacag aaatgcaaaa accagattct
     2641 tttatgctta aatatggttc caatgtaagt acagaaaaaa ggcaacaagt gttagaagat
     2701 ttcaaacagt actatgaaga aaacggtgga atattattcc aagagcctgg tgttgaaatc
     2761 gaacctttac ctaaaaaata cgtctctgaa gatatagtgg caagcgagaa tttaacaaga
     2821 gaaagagtag ctaacgtttt tcaattgccc tcaatattct taaatgcaag atcaaataca
     2881 aatttcgcga aaaatgaaga gttaaacaga ttttacttgc agcatacctt attgccaatc
     2941 gtcaaacagt atgaagaaga atttaatcgg aaactactta ctaaaacaga cagagaaaaa
     3001 aataggtatt ttaaatttaa cgttaaatct tatttaaggg ctgatagtgc aacacaagca
     3061 gaagtgtact ttaaagcagt tcgtagtggt tactacacta taaatgacat tagagagtgg
     3121 gaagatttac caccagttga aggtggagat aagccgctaa taagcggtga tttataccca
     3181 attgacacgc cacttgaatt aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa
     3241 agctaagtat tttcaaatga aaagaaaatc aaaaagtaaa ggtgaaatat tcatttatgg
     3301 tgatattgta agtgataaat ggtttgaaag tgatgtaact gctacagatt tcaaaaataa
     3361 actagatgaa ctaggagaca tcagtgaaat agatgttcat ataaattcat ctggaggcag
     3421 tgtatttgaa ggacatgcaa tatacaatat gctaaaaatg catcctgcaa aaattaatat
     3481 ctatgtcgat gccttagcgg catcaattgc taatgttatc gctatgagtg gtgacactat
     3541 ttttatgcac aaaaatagtt ttttaatgat tcataattca tgggttatga ctgtaggtaa
     3601 tgcagaagaa ttaagaaaga cagcggattt acttgaaaaa acagatgctg ttagtaattc
     3661 agcttattta gataaagcaa aagatttaga tcaagaacac ttaaaacaga tgttagatgc
     3721 agaaacttgg cttactgcag aagaagcctt gtctttcggc ttgatagatg aaattttagg
     3781 agctaatgaa atagctgcta gtatctctaa agagcaatat aagcgtttcg agaacgtccc
     3841 agaagattta aagaaagatg tagacaaaat cactaaaatt gatgatgtag atacatctga
     3901 attggttgaa acacctaaag aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat
     3961 taaacgcgaa tgcgaaattt taaaaatgac aatgaattat taggaggaaa tgaaatgccg
     4021 acattatatg aattaaaaca atccttaggt atgattggac aacaattaaa aaataaaaat
     4081 gatgaattga gtcagaaagc aacagatcca aatattgata tggaagacat caaacaacta
     4141 gaaacagaaa aagcaggttt acaacaaaga tttaacattg ttgaaagaca agtgcaagac
     4201 attgaagaaa aagaaaaagc gaaagttaaa gataaaggag aagcttatca atctttaagt
     4261 gataatgaga agatggttaa agctaaggca gagttttatc gtcacgcgat tttaccaaat
     4321 gaatttgaaa aaccttcaat ggaggcacaa cgtttattac acgctttacc aacaggaaat
     4381 gattcaggtg gagataagct cttaccaaaa acactttcta aagaaattgt ttcagaacca
     4441 tttgctaaaa accaattacg tgaaaaagct cgtctaacta acattaaagg tttagagatt
     4501 ccaagagttt catacacttt agacgatgat gatttcatta cagacgtaga aacagcaaaa
     4561 gaattaaaag caaaaggtga tacagtcaag ttcactacta ataaattcaa agtatttgct
     4621 gcaatttcag atactgtaat tcatggatca gatgtagatt tagtaaactg ggttgaaaac
     4681 gcactacaat caggattagc agctaaagag cgtaaagatg ccttagcagt aagtcctaaa
     4741 tctggattag aacacatgtc attttataat ggatctgtta aagaagttga gggagcagac
     4801 atgtatgatg ctattattaa cgctttagca gatttacatg aagattatcg tgataacgca
     4861 acaatttata tgcgatatgc agattatgtc aaaattatta gtgttctttc aaatggaaca
     4921 acaaatttct ttgacacacc agcagaaaaa gtatttggca aaccagtagt atttacagat
     4981 gcagcagtta aacctattgt gggagatttc aattattttg gaattaacta tgatggaaca
     5041 acttatgaca ctgataaaga tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg
     5101 tatgatcagc aacgtacatt agacagtgca ttcagaattg caaaagcaaa agaaaataca
     5161 ggtccattac ccagctaagc cccaaaaggt taatgtaaca gctaaggcta aatcagctgt
     5221 aatatcagcc gaataggggt gatgaaatga gtttagaaga aattaaattg tggttgagaa
     5281 ttgactataa tttcgaaaat gatttaattg aaggtctcat tcaatcggct aagtctgaat
     5341 tactattaag tggggttcca gattatgaca aagatgactt ggaatacccg cttttttgta
     5401 cagcgattaa atatatcatt gcaagagatt atgaaagtcg tggatactca aatgaccaat
     5461 ctagaagcaa ggtgtttaat gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt
     5521 ggtaggtgat ttttaaatgg aatttaatga atttaaagat cgcgcgtatt tttttcaata
     5581 tataaacaaa ggaccatatc cagatgaaga ggaaaaaatg aaattgtata gttgcttttg
     5641 taaaatttat aatccttcta tgaaagatag agaaatttta aaagcgactg aatcaaaatc
     5701 aggattaacc ataattgtca ggtcttctaa aactgaatat ctaccacaaa caaatcactt
     5761 agttaaaatt gacagtgcat tatattccga taaattattc aacattgtag aaataagaat
     5821 tgatacacca gatattggct ataatacagt ggttttatca gaaaaatgag tgtagaaatt
     5881 aaagggatac ctgaagtgtt gaataaatta gaatcggtat acggtaaaca agcaatgcaa
     5941 gctaagagtg atagagcttt aaataaagca tctgaatttt ttataaagac tttaaagaaa
     6001 gaattcgaga gttttaaaga tacgggtgct agtatagaag aaatgactaa atctaaacct
     6061 tatacaaaag ttggcagtca agaaagagct gttttaattg aatgggtagg tcctatgaat
     6121 cgcaaaaaca ttattcactt gaatgaacat ggttatacaa gagatggtaa aaaatataca
     6181 ccaagaggtt ttggagttat tgcaaaaaca ttagctgcta gtgaaaggaa gtatagagaa
     6241 attataaaaa aggagttggc cagataaatg aatatattaa acactgtaaa aggaatttta
     6301 ttatctgatg cagagctcca aacatatata aattctagaa tatactatta taaagtcact
     6361 gaaaatgctg aaacttccaa accttttgtt gttattacac ctatttatga tttaccttca
     6421 gactttatgt ctgataaata tcttagtgaa gaatacttaa ttcaaataga tgtagaatct
     6481 tcaaataatc agaaaacaat tgatataaca aaacgaataa gatacctgtt atatcaacaa
     6541 aatttaattc aagcatctag tcagttagat gcttattttg aagaaactaa acgttatgtg
     6601 atgtcgagac gttatcaagg cataccaaaa aatatatatt ataaaaatca gcgcatcgaa
     6661 taggtgtgct ttttaatatt taaggaggaa ataagcaatg gcagaaggac aaggttctta
     6721 taaagtaggt tttaaaagat tatacgttgg agtttttaac ccagaagcaa caaaagtagt
     6781 taaacgcatg acatgggaag atgaaaaagg tggtacagtt gacctaaata tcacaggttt
     6841 agcaccagat ttagtagata tgtttgcatc taacaaacgt gtatggatga aaaaacaagg
     6901 tactaatgaa gttaagtctg acatgagtat tttcaatatt ccaagtgatg atttaaacac
     6961 agttattgga cgtactaaag ataaaaatgg tacatcttgg gtaggagaga atacaagagc
     7021 accgtatgta acagtaattg gcgaatcgga agatggttta acaggtcagc cggtatatgt
     7081 agccttactt aaaggtactt ttagtttaga ttcaattgaa tttaaaacac gaggtgaaaa
     7141 agcagaagcc ccagaaccta caaaattaac aggtgactgg atgaatagaa aagttgatgt
     7201 tgatggaacg tcacaaggta ttgtatacgg ttatcatgaa ggtaaagaag gagaagcaga
     7261 attcttcaaa aaagtattcg ttggatacac tgacagagga gaacattctg atgattcttt
     7321 aggttcatta cccagctaat ccccaaaatg ttgaagtttc agttaattcg aaatctgcaa
     7381 cagtttcagc agaatagggg ctttcaaaaa aatcaaaagg agaataattt atgactaaaa
     7441 ctttaaaggt ttataaagga gatgacgtcg tagcctctga gcaaggcgaa ggcaaagtat
     7501 ctgtaacttt atctaattta gatgctgata caacatatcc aaaaggcact taccaagtgt
     7561 cgtgggaaga aaacggtaaa gaatctagta aagttgatgt acctcaattc aaaaccaatc
     7621 caattctagt ttcaggcgta tcatttacac cagaaactaa atcaattacg gtaaataccg
     7681 atgacaatgt tgagccaaac attgcaccaa gtacagcaac gaataaaaca ttgaaatata
     7741 caagtgaaca tccagagttt gttactgttg atgagagaac aggagcaatt cacggtgtag
     7801 ctgaaggtac ttcagtaatc actgctacgt ctactgatgg aagcgataag tcaggacaaa
     7861 tttcagtgac agtaacaaac ggataggaat ttaaggcgca gtatatctgc gtctttttta
     7921 tttgaataaa aggagctaat acaatgatta aatttgaaat taaagatcgt aaaacaggaa
     7981 aaacagagag ctatacaaaa gaagatgtaa caatgggcga agcagaaaaa tgctatgagt
     8041 atttagaatt agtaaatcaa gagaataaaa aagaagcacc taacgcaaca aaaatgagac
     8101 aaaaagagcg acagttatta gtagatttat ttaaagatga aggattgact gaagaagatg
     8161 ttttgaacaa gatgagcact aaaacttata caaaagcctt gaaagatata tttcgagaaa
     8221 tcaatggtga agatgaagaa gattcagaaa ctgaaccaga agagatggga aagacagaag
     8281 aacaatctca ataaaagaca ttttatcgaa cattaagaaa atacaacgtt tctgtatgga
     8341 gcagtatggg tggacattaa ctgaagtcag aaaacagccg tatgtaaaac ttttagaaat
     8401 acttaatgaa gagaataaag aagagactga agaaaaacaa agtgaacaaa aagtcattac
     8461 aggtacggat ttaagaaaac tttttggaag ctagaaagga ggttaatatg aatgaaaaag
     8521 tagaaggcat gaccttggag ctgaaattag accatttagg tgtccaagaa ggcatgaaag
     8581 gtttaaagcg acaattaggt gttgttaata gtgaaatgaa agctaatctg tcagcatttg
     8641 acaagtctga aaaatcaatg gaaaaatatc aggcgagaat taaggggtta aatgataggc
     8701 ttaaagttca aaaaaagatg tattctcaag tagaagatga gcttaaacaa gttaacgcta
     8761 attaccaaaa agctaaatcc agtgtaaaag atgttgagaa agcatattta aagttagtag
     8821 aagccaataa aaaagaaaaa ttagctcttg ataaatctaa agaagcctta aaatcatcga
     8881 atacagaact taaaaaagct gaaaatcaat ataaacgtac aaatcaacgt aaacaagatg
     8941 cgtttcaaaa acttaaacag ttgagagaag ctgagcagaa gcttaagaat agtaaccaag
     9001 ctactactgc acaactaaaa agagcaagtg acgcagtaca gaagcagtcc gctaagcata
     9061 aagcacttgt tgaacaatat aaacaagaag gtaatcaagt tcaaaaacta aaagtgcaaa
     9121 atgacaatct ttcaaaatca aatgataaaa ttgaaagttc ttacgctaaa actaatacta
     9181 aattaaagca aacagaaaaa gaatttaatg atttaaacaa tactattaag aatcatagcg
     9241 ctaatgtcgc aaaagctgaa acagctgtta ataaagaaaa agctgcttta aataatttgg
     9301 agcgttcaat agataaagct tcatccgaaa tgaagacttt taacaaagaa caaatgatag
     9361 ctcaaagtca tttcggtaaa cttgcaagtc aagcggatgt catgtcaaag aaatttagtt
     9421 ctattggaga caaaatgact tccctaggac gtacgatgac gatgggcgta tctacaccga
     9481 ttactttagg gttaggtgca gcattaaaaa caagtgcaga cttcgaaggg caaatgtctc
     9541 gagttggagc gattgcacaa gcaagcagta aagacttaaa aagcatgtct aatcaagcgg
     9601 ttgacttagg cgctaaaaca agtaaaagtg ctaacgaagt tgctaaaggt atggaagaat
     9661 tggcagcttt aggctttaat gccaaacaaa caatggaggc tatgccaggt gttatcagcg
     9721 cagcagaagc aagtggtgca gaaatggcta caactgcaac tgtaatggct tcagcgatta
     9781 actctttcgg tttaaaagca tctgatgcaa accatgttgc tgatttactt gcgagatcag
     9841 ctaatgatag tgctgcagat attcaataca tgggagatgc attaaaatat gcaggtactc
     9901 cagcaaaagc attaggagtt tcaatagagg acacttctgc agcaattgaa gttttatcta
     9961 actcagggtt agaggggtct caagcaggta ctgcattaag agcttcgttt attaggctag
    10021 ctaatccaag taaaagtaca gctaaggaaa tgaaaaaatt aggtattcat ttgtctgatg
    10081 ctaaaggtca atttgttggc atgggtgaat tgattagaca gttccaagac aacatgaaag
    10141 gcatgacgag agaacaaaaa ctagcaacag tggctacaat agttggcact gaagcagcaa
    10201 gtggattttt agccttgatt gaagcgggtc cagataaaat taatagctat agcaaatcat
    10261 tgaagaactc taatggtgaa agtaaaaaag cagctgattt gatgaaagac aacctcaaag
    10321 gtgctctgga acaattaggt ggcgcttttg aatcgttagc aattgaagtt ggtaaagatt
    10381 taacgcctat gattagagca ggtgcggaag gattaacaaa attagttgat ggatttacac
    10441 atcttcctgg ttgggttaga aaggcttcgg taggcttagc aatttttggt gcatctattg
    10501 gtcctgctgt tcttgctgga ggcttattaa tacgtgcagt tggaagcgcg gccaaaggat
    10561 atgcatcatt aaatagacgc attgctgaaa atacaatact ttctaatacc aattcaaaag
    10621 caatgaaatc tttaggtctt caaaccttat ttcttggttc tacaacagga aaaacgtcaa
    10681 aaggctttaa aggattagcc ggagctatgt tgtttaattt aaaacctata aatgttttga
    10741 aaaattctgc aaagctagca attttaccgt tcaaactttt gaaaaacggt ttaggattag
    10801 ccgcaaaatc cttatttgca gtaagtggag gcgcaagatt tgctggtgta gccttaaggt
    10861 ttttaacagg acctataggt gctacaataa ctgctattac aattgcatat aaagttttta
    10921 aaaccgcata tgatcgtgtg gaatggttca gaaacggtat taacggttta ggagaaacta
    10981 taaagttttt tggtggcaaa attattggcg gtgctgttag gaagctagga gagtttaaaa
    11041 attatcttgg aagtataggc aaaagcttca aagaaaagtt ttcaaaggat atgaaagatg
    11101 gttataaatc tttgagtgac gatgaccttc tgaaagtagg agtcaacaag tttaaaggat
    11161 ttatgcaaac catgggcaca gcttctaaaa aagcatctga tactgtaaaa gtgttgggga
    11221 aaggtgtttc aaaagaaaca gaaaaagctt tagaaaaata cgtacactat tctgaagaga
    11281 acaacagaat catggaaaaa gtacgtttaa actcgggtca aataacagaa gacaaagcaa
    11341 aaaaactttt gaaaattgaa gcggatttat ctaataacct tatagctgaa atagaaaaaa
    11401 gaaataaaaa ggaactcgaa a
//
LOCUS       DBNENW010000082        11321 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNENW010000082 DBNENW010000000
VERSION     DBNENW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.082"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            24..731
                     /gene="deoD"
                     /locus_tag="AC1LW8_001725"
     CDS             24..731
                     /gene="deoD"
                     /locus_tag="AC1LW8_001725"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP4773555.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVGTARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            738..2090
                     /gene="tet(38)"
                     /locus_tag="AC1LW8_001726"
     CDS             738..2090
                     /gene="tet(38)"
                     /locus_tag="AC1LW8_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HIP4773556.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            2171..2833
                     /gene="deoC"
                     /locus_tag="AC1LW8_001727"
     CDS             2171..2833
                     /gene="deoC"
                     /locus_tag="AC1LW8_001727"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIP4773557.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKVAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            2861..4039
                     /gene="deoB"
                     /locus_tag="AC1LW8_001728"
     CDS             2861..4039
                     /gene="deoB"
                     /locus_tag="AC1LW8_001728"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIP4773558.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(4170..4985)
                     /gene="phnE"
                     /locus_tag="AC1LW8_001729"
     CDS             complement(4170..4985)
                     /gene="phnE"
                     /locus_tag="AC1LW8_001729"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIP4773559.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAIESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(4982..5782)
                     /gene="phnE"
                     /locus_tag="AC1LW8_001730"
     CDS             complement(4982..5782)
                     /gene="phnE"
                     /locus_tag="AC1LW8_001730"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIP4773560.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(5784..6557)
                     /gene="phnC"
                     /locus_tag="AC1LW8_001731"
     CDS             complement(5784..6557)
                     /gene="phnC"
                     /locus_tag="AC1LW8_001731"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIP4773561.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(6771..7727)
                     /locus_tag="AC1LW8_001732"
     CDS             complement(6771..7727)
                     /locus_tag="AC1LW8_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIP4773562.1"
                     /translation="MKKFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            7956..9500
                     /locus_tag="AC1LW8_001733"
     CDS             7956..9500
                     /locus_tag="AC1LW8_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIP4773563.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITDWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            9551..11086
                     /locus_tag="AC1LW8_001734"
     CDS             9551..11086
                     /locus_tag="AC1LW8_001734"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIP4773564.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            11155..11321
                     /locus_tag="AC1LW8_001735"
                     /pseudo
     CDS             11155..11321
                     /locus_tag="AC1LW8_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
BASE COUNT         3747 a         1818 c         2121 g         3635 t
ORIGIN      
        1 acaaatattg ggaggaatat tttatgaaat caacaccaca cattaaacca atgaatgacg
       61 tcgaaattgc agaaacggtt ctattgccag gagatccgtt aagagctaag ttcattgcag
      121 aaacttattt ggatgatgtg gaacagttca atacagtgcg aaacatgttt ggttttaccg
      181 gaacatataa aggtaaaaaa gtttctgtca tgggttcagg tatgggtatg ccatctattg
      241 gcatttactc ttatgaatta attcatacat ttggttgtaa aaaattaatt cgcgttggtt
      301 cttgtggcgc gatgcaagaa aacattgatt tatatgatgt gattattgca caaggtgcct
      361 ctactgattc aaattacgtt caacaatatc aattaccagg tcattttgcg ccaattgctt
      421 cttatcaatt attagaaaaa gcagttggaa cagcacgtga caaaggtgta cgtcatcatg
      481 taggtaatgt gttatcaagt gatattttct ataacgcgga tacaacagcg agtgaacgtt
      541 ggatgcgtat gggtatttta ggtgtagaaa tggaatcagc tgcattatac atgaatgcaa
      601 tttacgctgg tgtcgaagca ttaggtgtgt tcacagtgag cgatcattta attcatgaaa
      661 cgtcaacaac acctgaggaa agggaacgtg catttacaga tatgattgaa attgcactgt
      721 cattggttta gataattatg aatgttgaat attctaaaat aaagaaagca gtacctattt
      781 tattattctt atttgtattc agtttggtta tagacaactc atttaaattg atttctgtag
      841 ccattgctga tgacttaaac atttctgtaa cgacagtaag ttggcaagcg acattagccg
      901 gtttagtaat tggtattggc gctgtagtat acgcttcatt atctgatgcc attagtatac
      961 gcacactatt tatttatggc gtgatattaa tcattatcgg atcaattatt ggttacattt
     1021 tccaacatca attcgcatta cttttagttg gacgtattat tcaaactgcc ggtttagccg
     1081 ctgcagagac attatatgtg atatatgttg caaagtatct ttctaaagag gaccagaaga
     1141 cttaccttgg cttaagtacg agcagttatt ccttgtcatt agttatcggt acattatcag
     1201 gtggatttat ttctacgtat ttacactgga caaatatgtt tttaattgca ttaatcgtag
     1261 tatttacgtt gccattccta tttaaattat taccaaaaga aaataatacg aataaagctc
     1321 atttagattt tgttggctta attctagtgg caactattgc tacaacagtc atgctgttta
     1381 ttacgaactt taattggtta tatatgattg gtgccttaat tgcgattatc gtttttgcgc
     1441 tatatattaa aaatgcgcaa cgtccattag taaataaatc atttttccaa aataaacgtt
     1501 atgcttcatt tttatttata gtatttgtaa tgtatgctat ccaattgggt tatattttta
     1561 cgttcccatt cataatggag caaatttatc atctgcaact agacacaaca tcactgttat
     1621 tagtaccggg ttatatagta gcagtcatcg ttggtgcact aagtggtaaa atcggcgaat
     1681 atctgaattc aaaacaagcg attatcacag caattatttt aatcgcactg agcttgattt
     1741 tacctgcatt tgcagtaggt aatcacattt caatcttcgt catttctatg atattctttg
     1801 caggtagctt tgctttaatg tatgcacctt tacttaacga agccattaaa acaatagatc
     1861 ttaatatgac aggtgtggct attggttttt ataatttaat tattaatgtg gcggtatctg
     1921 taggtattgc gattgctgcg gctctaatcg attttaaagc attaaatttc ccaggcaatg
     1981 atgcattaag ttcacatttc ggtattattt taattatttt aggtttaatg agtattgtcg
     2041 gattagtttt attcgtcagc ttaaatcgtt ggacacaatc tgaaaaataa atagatataa
     2101 attcgcgaga tatattcgta tttatagtaa aattaaataa agagattata taacacgagg
     2161 agtagtaagt atgaaatttg agaaatatat agatcacact ttattgaagc ctgagtcaac
     2221 acgtacgcaa atcgatcaaa tcatcgatga agcgaaagca tacaatttta aatctgtatg
     2281 tgtgaatcca acacatgtta aatatgcagc agagcgacta gctgattcag aggtgctcgt
     2341 ttgtacggta ataggattcc cattaggtgc gtcgacaact gcaacgaaag catttgaaac
     2401 agaagatgca attcaaaatg gtgcagatga aattgacatg gtcatcaaca tcggcgcatt
     2461 aaaagatgga cgttttgatg atgtacaaca agacattgaa gcagtggtta aagttgcgaa
     2521 aggtcacaca gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa
     2581 agcgagtgaa ttaacaaaag cggctggtgc ggacttcgtt aaaacttcaa caggttttgc
     2641 aggtggcggt gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt
     2701 agaagtaaaa gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc
     2761 aggtgcgaca cgtattggtg cgagcgcagg tgttcaaatt atgcaaggtt tagaagcaga
     2821 ttcagattac taatatatat aaattttggg agtgatagct atgacaagac catttaatcg
     2881 tgtacattta atcgtaatgg attcagtagg tattggtgaa gcgccagacg cagctgattt
     2941 taaagatgaa ggttcacata ctttaagaca taccttagaa ggtttcgatc aaactttacc
     3001 aaaccttgaa aagttaggtc tagggaacat cgataaatta ccagtagtaa atgcagttga
     3061 acaaccagaa gcatactata ctaaattgag tgaagcttca gttggtaaag atacaatgac
     3121 tggtcactgg gaaattatgg gtttaaatat tatgcaacct tttaaagtat accctaatgg
     3181 attccctgaa gagttaattc aacaaattga agaaatgaca ggtcgtaaag ttgttgctaa
     3241 caaaccggca tcgggtacgc aaattatcga tgagtggggc gagcaccaaa tgaaaactgg
     3301 tgacttaatt gtttatacaa gtgcagaccc agtattgcaa attgctgcac atgaagacat
     3361 tatcccatta gaagagttat atgatatttg tgaaaaggtt cgtgagttga caaaagaccc
     3421 taaatattta attggtcgta ttatcgcacg tccatatgtt ggtgaaccag gaaactttac
     3481 acgtacatct aatcgacatg actatgcgtt aaaacctttt ggtaaaactg tcttagatca
     3541 tttgaaagac ggtggttatg atgttattgc catcggtaaa attaatgaca tttatgatgg
     3601 tgaaggtgta acagaagcgg ttcgtacgaa gagtaacatg gacggtatgg atcaattgat
     3661 gaaaattgtt aagaaagatt tcacaggtat tagcttctta aacttagtag actttgatgc
     3721 attatacggt catcgtcgtg ataaaccagg ttatgcacaa gcaattaaag atttcgatga
     3781 tcgcttgcca gaactgttta gcaacttaaa agaagacgat ttagtaatta ttacagcaga
     3841 ccatggtaat gacccgacag cgccaggtac ggaccatacg agagaatata tcccagtaat
     3901 tatgtacagt ccgaaattta aaggtggtca tgcactagaa agtgatacta cattcagttc
     3961 tatcggtgca actatagcag ataatttcaa cgtaacatta ccagagttcg gtaaaagtta
     4021 tttaaaggaa ttgaaataga ataaatttag atataataaa aacagcagtg aagttaacta
     4081 taacaatagt tttcttcact gctgttttta ttataataga gaaacgtaag acggtaggac
     4141 ctcttattta ggagtatcct gatttaatgt taaacaatac gttttcggat tgaaccggaa
     4201 attaaatcta caattgcgac cattagtact aaaccgatta atataatacc tacacggtcc
     4261 caagaacgtg tttgaatggc aaatatgagt ggtgtcccga taccaccagc cccaattagc
     4321 cccagtatag aagctgaacg taagtttagt tcaaagcgat aaagtatgag agatagaaag
     4381 gcaggcataa tttgtggtat gactgcaaat acgagtgttt taatcttatt cgcaccactg
     4441 gcctttaatg attctatagc actgaaatct agaccttcaa tatcttcagc taaaagtttc
     4501 ccaagcatac ctacagaatg gatacctaaa gctaatacac ctgaaaatga acctgggcca
     4561 acagctttga taaatataag tgccattaca atttctggga agacacgtat aacacttaaa
     4621 ataaatttgc taacacctga aaccgggcgt aactttacca tattatttgc acctagaaat
     4681 gctaatggaa tacagataat tgcggcgatg aaagtaccta caacggctat cgcaaaggtt
     4741 tcaagtaaac cacgtaataa gtcttcgcca tctggtatat agatatagct gatgtcagga
     4801 tggaataatc cgctgaatat ggattttaag atttctaatg atttactttt aagttctaaa
     4861 cttggtacac ctgcaaatgc ccagatgata atagctaaga caacaattgc aataagccat
     4921 cttttaatca attttcgttt gtgtgctttt gtgtgaacat tatattttgc tatttcctgt
     4981 gtcatgcgag atgtgccctc actttcgtac tgatgtaatc aatgacgacg acaataacta
     5041 aagtaaataa aataatcgtt gctgtttttg gatattgaaa taaaccaagt gtttgatcat
     5101 aaaacaatcc aataccgcca gcgccgacta atccaagcac agctgaagca cgtatattta
     5161 cttcaaatgc atataatacg tatgacataa atgacgatat ggcttgtggt acaacaccga
     5221 aaacaatcca ttttatttta ttagcgccaa cagccgtcat tgcttccatt ggacctggat
     5281 ctatcgtttc caatgattca tataataatt ttccaataat acagatagtt aaaataaaca
     5341 gtgctaatat ccctggaatt tgaccgattc caaatacagc cacaaagatt gctgctaata
     5401 gcaaatccgg tatagtacga actatattta aaataaaacg cgagggtatt gaaatccact
     5461 tttgatgaac gatattgcta gcacataata acgcaattgg tattgaaacg atgctaccta
     5521 atactgtact tacgatagcc attcgaatag tatctaacat tggcgttgta atttgttgta
     5581 aatactcgaa atcaggtgga atcatttgtt tgaatagatc acctatttga ggtattccta
     5641 tcattaaatc tccaaaatta aaccccgtat aaatgaagct ccaaatgata agcacaatga
     5701 ttaacatgaa ggtaaaactc gtttttaaag aaaccttttt ctttaaaagg gagtcatact
     5761 ttgtaggtgt ttctaaaggc atgttagttc actcctagct tttcatcttc tttaattgta
     5821 cgtccatata tttcactaaa tacgtcatct gttgcttcag atgcaggacc atcatagaca
     5881 acctcaccat cacgtaaacc aatgatgcgt gtgccatatt cttttgctaa gtcaacaaaa
     5941 tgtaagttaa ttaaaattgt gatgcctaat tcttggttga tttttcttaa atcatccata
     6001 acctgtttcg tagttaatgg gtctaatgaa gcaactggct catctgcaag aataatttca
     6061 gattcttggc atagtgcacg tgcaatagat atacgttgtt gttgacctcc tgataattca
     6121 tcagagcgtt gattatattt atctaagatg ttgacccgtt ctagtgcatc cattgcttta
     6181 attttgtctt cttttgggaa taaacctaat accattttcc aagtagggtg ataacctaca
     6241 cgtccactta gtacatttcg taatacactt gaccgtttaa ctaaattaaa atgttggaaa
     6301 atcataccta tatttcggcg catttctaat agtgctttac catgggcttt agtgattgat
     6361 ttaccttgga tgaaaatttc acctgacgtg atatcatgca aacgatttac agatcttaat
     6421 aacgtggatt tcccagcacc agatagtccg acaatgactg caaattcacc tttttcaata
     6481 tttaagttaa tatttttcaa gcctacatga ccgttaggat agactttact gacgttttta
     6541 aattcgattt gactcatgtt tgacaccttt ctttaataaa gaaaagccaa ggtaaactga
     6601 aatatcatag acattgggtt tctgtgtaca aatggtttca aatgtacagc gacacctttc
     6661 tatatctgtt tcagtttagc gcctagcttt tcaatattaa tctagaatat atctattgaa
     6721 cgaaagcttt taataccaaa ttcgctaatg attcatttgt taaataatga ttatttcata
     6781 tctttaacta atttttcgta ctctcttaca atgtcgaaat ttgaatcttt cgtttctgtg
     6841 tatccttcat gtgaataaac ttcgctaata attttgtgac cttcttttga tttagcaatg
     6901 tctataaaag cttttttcaa tttttcttgg aaatctttat ccatatctgg tcttacagaa
     6961 attgtgtcat tcggaatagc ttgtgttaat tttaaaattc gtgtgtcttt aaatacattt
     7021 ggttggtctt ttttcacagt attacgtgca tcgttaaata cagccgcagc atctacatct
     7081 ccatttaata atgagataac tgcttggtca tgacctttaa cattcacaat tttcatatct
     7141 ttagttgcat taatacctgc ttcgtttttt aacatcgcaa gtgggaatgt atatccagca
     7201 gttgatgtta catcttgtaa ggcaattttc ttacctttta aatctttcaa gcttttaatt
     7261 tttgagtctt ttttaacaag aatttctgac ttataactat ctacaagttc tttacttgct
     7321 gaaccatctt cttttacacc gaaacgttgt gcttgtaata ataaatcagc tgctttttga
     7381 tcatgtgcta atgtgtatgc cgttggtggt aagaaaccaa catcaacttt tttagacttc
     7441 atagcttcaa caattgtatt gtagttagtt gatacagaca ctttaactgg aatccctaat
     7501 tctttagata gtaatttttc taatggtttt gctttagcct ctaatgttcc agcattttgc
     7561 gaaggtacaa attgtactgt taattcttta ggtttgtatc ctcctgattt agaatccgaa
     7621 tcattactag cgttcttttg attatctaaa gaacttgaat ttccacatgc tgctgcaaaa
     7681 acaatgactg ctaacattaa tacaaataaa cacttaaatt ttttcatttg ataactgtcc
     7741 ccttcacatt cttaatcgtg taatatgtat gcaatgaaat tacacttata gcgtaccaca
     7801 tttaaatgcg aaaaagtatt ttataaaaat gaattttaat aactttattt aagagtttga
     7861 taagattttg taaatttttc gttaaattgc ccatttcaaa catatattta tcctataatt
     7921 caagaagaaa tttattttaa aaggagcctc acaaaatgaa aaaaatatat aagtcattaa
     7981 ctgtctctgc aattgttgca acggtatcat taagtgcttt accgcaatct ttagctataa
     8041 cgcatgaatc gcaacctaca aagcaacagc gaacggtatt attcgatcgt tctcatggtc
     8101 aaacagctgg tgctgcagat tgggttagtg atggtgcatt ttcagattat gcggattcaa
     8161 tacaaaaaca aggttatgac gttaaagcta ttgatggtca ttcgaacata acagaagcaa
     8221 gtttgaaaag ttctaaaata tttgtaattc ctgaggctaa tattcctttc aaagaatcag
     8281 aacaggcagc aattgttaac tatgtgaaac aaggtggcaa tgttgtcttt atttcagatc
     8341 attacaatgc tgaccgaaat ttaaatcgta ttgattcatc agaggcaatg aatggttatc
     8401 gacgtggagc atatgaagat atgtcgaaag gtatgaatgc agaagaaaaa agttctactg
     8461 caatgcaagg tgtgaaaagt tcagattggt tatctacaaa ctttggcgta cgttttcgat
     8521 ataatgcact aggtgattta aatacgagca atattgtttc ttcaaaagag agtttcggta
     8581 ttactgaagg tgtgaaatct gtctctatgc atgccggatc aacattagca attactaatc
     8641 cagagaaagc aaaaggtatt gtgtatacac cagaacaatt gccagcgaaa agtaaatggt
     8701 cacatgctgt agatcaaggt atttataatg ggggcggtaa agcagaaggc ccctatgtag
     8761 caatttctaa agttggaaaa ggtaaagcag catttatcgg tgattcatca cttgtggaag
     8821 atagttcgcc caaatatgta agagaagata atggagaaaa gaagaaaaca tatgatggtt
     8881 ttaaagaaca agacaacggt aagctattaa ataatataac ggattggatg tctaaagata
     8941 gtgatgggaa atcacttaag gcgagtggac taacattaga tacaaagact aagttgcttg
     9001 attttgaacg accagagcgt tcaactgagc ctgaaaaaga gccatggtca caaccgccga
     9061 gtggttataa atggtatgat ccaacaacat ttaaagcagg tagttatggc agcgaaaaag
     9121 gcgcagatcc tcagccaaac acaccagatg atcatacgcc accaaatcag aacgaaaaag
     9181 taacatttga tatcccgcaa aatgtttctg taaatgagcc atttgaaatg acaatacatt
     9241 taaaaggatt tgaagcaaat caaacacttg aaaatcttag agttggtatt tacaaagaag
     9301 gcggacgtca aatcggacaa ttttcaagta aagataacga ttataaccca ccaggttaca
     9361 gtactttgcc aacagttaaa gcagatgaaa acggaaatgt cacaattaag gtcaatgcta
     9421 aagtacttga aagtatggaa ggttcaaaga ttcgtttaaa actcggtgac aaaaccttga
     9481 ttacaacaga cttcaaataa atatataata gaaataagaa agatgtttgt gattgaagga
     9541 gtgagtgagg atgtcaaaca tagcatttta tgtcgtgagt gacgtacatg gttatatttt
     9601 cccaacagat tttacgagta gaaatcaata tcaacctatg ggattgttac tagcgaatca
     9661 tgttatagaa caagacagaa ggcagtatga ccaaagtttt aaaatagata atggtgattt
     9721 tttgcaaggg tcaccatttt gtaattactt aatcgcgcat agcggcagta gccagccttt
     9781 agttgatttt tataatcgaa tggcattcga ctttggtacg cttggtaatc atgaatttaa
     9841 ttatggattg ccatacttaa aagacacttt acgcagactc aattatccag ttttgtgcgc
     9901 taatatatat gaaaatgata gtacattgac tgataacggt gtgaagtatt ttcaggttgg
     9961 agatcaaact gttggtgtga taggtttaac gacacaattt attccccatt gggaacaacc
    10021 agagcatatt cagtcactta cgtttcatag tgcttttgaa atacttcaac aatacttacc
    10081 tgaaatgaag cgacatgcag atatcattgt ggtttgttac catggtggat ttgaaaagga
    10141 tttagaaagt ggtacgccga ccgaagtatt aacgggtgaa aatgagggat atgccatgtt
    10201 agaagcgttt tctaaagata tagatatctt tattacgggt caccaacatc gacaaattgc
    10261 tgaaaggttt aagcaaacgg ctgtgattca acctggtacg agaggtacaa ctgtaggcag
    10321 agtagtcttg agtactgatg aatatgaaaa tctatccgtt gaatcatgtg aattacttcc
    10381 tgttatagat gattccacat ttactattga tgaagatgac caacatttac gaaagcagtt
    10441 agaggactgg ttagattacg aaattactac attgccatat gatatgacga ttaatcatgc
    10501 atttgaggca cgtgtggcac cgcatccttt tacaaatttt atgaattacg ctttattaga
    10561 aaaaagtgac gcagatgttg cctgtacagc tttgtttgat tctgctagtg gtttcaagca
    10621 agtcgtgacg atgcgagatg ttattaacaa ttacccattt ccaaatacat ttaaagtttt
    10681 agctgtaagt ggtgccaaac ttaaagaagc cattgaacga tcagcagaat attttgacgt
    10741 gaaaaatgat gaagttagtg tgagcgcaga cttccttgga cccaaaccac aacactttaa
    10801 ttatgatata tatggtggcg taagttatac cattcatgtt ggaagaccaa agggacaacg
    10861 tgtgagcaat atgatgattc aaggtcacgc agttgattta aagcagacat atacaatttg
    10921 tgtaaataat tatcgtgcag ttggcggtgg tcagtatgat atgtttatcg acgcgccagt
    10981 tgtgaaagat attcaagttg aaggcgcaca attacttatt gattttttat caaataataa
    11041 tttgatgcgt atcccgcaag ttgttgattt taaagttgaa aagtgacgga tatatgaaaa
    11101 acttacttga ttttaatcgg gtaagttttt gtttaaagcg agaaagtatt atctttagac
    11161 gacgtgattg aaatgtcacg tcctgtcaaa atgaatagtt tattaaagtt gtattttgat
    11221 agaaatttac tgacaccaca aaaattattg aattatttaa aagttgatga aacatttttg
    11281 aatcatctag caggtattaa tttaaaactc tttaaggatt a
//
LOCUS       DBNENW010000083        11318 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNENW010000083 DBNENW010000000
VERSION     DBNENW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.083"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..945
                     /gene="hlgC"
                     /locus_tag="AC1LW8_001736"
     CDS             <1..945
                     /gene="hlgC"
                     /locus_tag="AC1LW8_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HIP4773565.1"
                     /translation="LKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEII
                     KRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAM
                     RWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFN
                     YSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDP
                     RDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHY
                     GNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            947..1924
                     /gene="hlgB"
                     /locus_tag="AC1LW8_001737"
     CDS             947..1924
                     /gene="hlgB"
                     /locus_tag="AC1LW8_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HIP4773566.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1976..2443)
                     /locus_tag="AC1LW8_001738"
     CDS             complement(1976..2443)
                     /locus_tag="AC1LW8_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIP4773567.1"
                     /translation="MTSKDITQISVIAAILTILAALKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2454..3146)
                     /locus_tag="AC1LW8_001739"
     CDS             complement(2454..3146)
                     /locus_tag="AC1LW8_001739"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HIP4773568.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYISFL
                     ESTPKQVVYERV"
     gene            complement(3157..4272)
                     /locus_tag="AC1LW8_001740"
     CDS             complement(3157..4272)
                     /locus_tag="AC1LW8_001740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4773569.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSAFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4250..5257)
                     /gene="bioB"
                     /locus_tag="AC1LW8_001741"
     CDS             complement(4250..5257)
                     /gene="bioB"
                     /locus_tag="AC1LW8_001741"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIP4773570.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(5259..6617)
                     /gene="bioA"
                     /locus_tag="AC1LW8_001742"
     CDS             complement(5259..6617)
                     /gene="bioA"
                     /locus_tag="AC1LW8_001742"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIP4773571.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEETIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6595..7281)
                     /gene="bioD"
                     /locus_tag="AC1LW8_001743"
     CDS             complement(6595..7281)
                     /gene="bioD"
                     /locus_tag="AC1LW8_001743"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIP4773572.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(7336..7503)
                     /locus_tag="AC1LW8_001744"
     CDS             complement(7336..7503)
                     /locus_tag="AC1LW8_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773573.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(7734..9467)
                     /locus_tag="AC1LW8_001745"
     CDS             complement(7734..9467)
                     /locus_tag="AC1LW8_001745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773574.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9492..11255)
                     /locus_tag="AC1LW8_001746"
     CDS             complement(9492..11255)
                     /locus_tag="AC1LW8_001746"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773575.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         3754 a         2061 c         1539 g         3964 t
ORIGIN      
        1 cttaaaaata aaatattagc cacaacttta tctgtgagct tacttgcccc tcttgccaat
       61 ccgttattag aaaatgctaa agctgccaac gatactgaag acatcggtaa aggaagcgat
      121 atagaaatta tcaaaaggac agaagataaa acaagtaata aatggggcgt gactcaaaat
      181 attcaatttg attttgtgaa ggataaaaaa tataacaaag atgctttgat attaaagatg
      241 caaggattca ttagctctag aacaacatat tacaactata aaaaaactaa tcatgttaaa
      301 gctatgcgat ggccattcca atataatatt ggtttaaaaa caaatgataa atatgtttct
      361 ttaattaatt atttacctaa aaataaaatt gaatctacaa acgtgagtca gacattagga
      421 tacaatatcg gtggtaattt ccaatcagcc ccatcactcg gtggtaatgg atcatttaac
      481 tattctaaat cgattagcta tacacaacaa aattatgtaa gtgaagtaga acaacaaaac
      541 tcaaaaagtg ttttatgggg cgtcaaagcg aattcattcg ccactgaatc aggtcaaaaa
      601 tcagcatttg atagcgattt atttgtaggc tacaaacctc atagtaaaga tcctagagat
      661 tatttcgttc cagacagtga gttaccacct cttgtacaaa gtggatttaa cccttcattt
      721 atcgccacag tatctcatga aaaaggttca agcgatacaa gcgaatttga aattacttac
      781 ggaagaaaca tggatgtcac tcatgccatt aaaagatcaa cgcattatgg caacagttat
      841 ttagacggac atagagtcca taatgcattt gtaaatagaa actatactgt gaaatacgag
      901 gtcaattgga agactcatga aatcaaggtg aaaggacaga attgatatga aaatgaataa
      961 attagtcaaa tcatccgttg ctacatctat ggcattatta ttactttctg gtactgctaa
     1021 tgctgaaggt aaaataacac cagtcagcgt aaaaaaagtc gatgacaaag ttactttata
     1081 caaaacaaca gccacagcag attctgataa atttaaaatt tcacagattt taacatttaa
     1141 tttcatcaaa gataaaagtt atgataaaga tactttagta cttaaagcta ctgggaatat
     1201 taactcaggc tttgtgaaac ctaatcctaa tgactatgac ttttcaaaat tatattgggg
     1261 agctaaatac aatgtatcta taagctcaca atctaatgat tcagtaaacg tcgttgatta
     1321 tgcaccaaaa aatcaaaatg aagagtttca agttcaaaat actttaggct atacatttgg
     1381 tggtgacatt agtatctcta atggtttatc tggtggactt aatggaaata cagctttttc
     1441 tgaaacaatt aattataaac aagaaagtta cagaacaaca ttaagtcgca acacaaatta
     1501 taaaaatgtt ggctggggag ttgaagcaca taaaattatg aataatggtt ggggacctta
     1561 tggacgagat agcttccacc caacatatgg taatgaactc ttcttagctg gcagacaaag
     1621 cagtgcatac gctggccaaa acttcatagc gcaacaccaa atgccattat tatctagaag
     1681 taacttcaat ccagaatttt taagcgtact atcacacaga caagatggcg ctaaaaaatc
     1741 taaaattaca gtaacttatc aacgtgaaat ggatttatac caaattcgtt ggaatggctt
     1801 ctactgggca ggcgcaaatt ataaaaactt taaaactaga acatttaaat caacatatga
     1861 aattgattgg gaaaatcaca aagtgaaatt gttagataca aaagaaactg aaaacaataa
     1921 atagctagta aaacacggtc gccaacagta attgtgacga ccgtgttttg atttattatc
     1981 ttagtaagac tgccattctt tttctcaatg tgagatataa aggaatagct acaattaaag
     2041 tgaatattac gcctggaatc gcgtttaaca acactaccca cacaggtaaa tttaaaataa
     2101 tagataatag tagcctagat accaaactgc ctaatacact tgctaaaact aatgatagta
     2161 catttatttt caataaataa acaactgcaa tagctataac tctaaatata atagaaataa
     2221 tcacattaat tggattaaat acgccaaata ctagtaataa taggctagat aataatccac
     2281 ctaaaaagta ctttttaatt ccaaagaaag ctaatatcaa taatgctgcc ggtgcagata
     2341 attgaaaatc taatcctggt ataatggacg gtattttcaa agctgccaaa atggttaaaa
     2401 tcgcagcaat gacactaatt tgagtaatat cttttgatgt catactaaaa cccctatacc
     2461 ctttcataaa caacttgctt cggtgtgctt tctaaaaatg atatgtaatg atttaaatca
     2521 atacgatcgt ccacaaatat tattctgcct ccatatctcg tattaactgg tttaatatca
     2581 aataatcgat ggtaaccaat tttagcagag gcaaaataac ctgtcgtata cgttaagtca
     2641 tctgacacac aaagttctcc tttgacataa ggatgcgcat tgatacaact agcaattgct
     2701 aaggcatcag tcactctttc attaagatca cctttattat ttatatcttc aaacgaaaaa
     2761 tgtgttgccc taatccccct ttgtccaaat gaatctaaac gtttaccaga tatagcagat
     2821 agaataatag ctcctgtata aaccgtttca tttttaatat atgtcatccc ttgatttagc
     2881 gcttgttcag tgacaccaca ttcttgtgtt aaatgttgga gatttgcttt atcatcctca
     2941 ataatttgta atgcttttat ttgttgaatc ggttccatga ttttttgtat ttttatattg
     3001 agaaaatccg gttgcccatt ttcatgaaaa aaacctttat tataaaacct ttgtaccgtc
     3061 tgttctattt tgtcaaactc acaaatcgtt tcagcaccac taatatgaac atcttgattg
     3121 ctagaacgca tttttatact atacatgacg atcacctcaa tcttcttgat gcaaaatttc
     3181 aaacaaccta tctatatctt gttcagtatg aaaatacgac aatgatattc ttaacattgg
     3241 cttagtcaca gttggatacc ttaaataact tgtaaacaca tgatgcttta ataatgtttg
     3301 atgaatgttc tcagccgctt ctatgtcatc aaactcaata aacttaatcg gcgagtttga
     3361 actattataa ttaacattga gtgcttttaa cttttggtta aaatatttac tcaaactatt
     3421 taatttagtg cgtctatcat cagcatttat taacttttca atgtttcttt ttataaaata
     3481 caaattataa attggcaaac tacttgagta gatgagtggt ctaccgtgat taattaacat
     3541 atccttcaca tcatttgaac ttaaaatcac acccccgtat gcaccacatg ctttagataa
     3601 actagaagtg agtatatcta caccttgata attcgagtaa ttctctattc caaaactatg
     3661 tgaaacatcg agtatcagtg ttgcgttata tttatgcttt aatgagacta attgaccaat
     3721 atccacaacg tcgccattcg ttgaaaatgc actatcagat atgattattt ttggtatatt
     3781 ttgattaggg tatttctcta accttttttc taaatcagca atatctaaat gcttatatat
     3841 cactttttct aaaccactta acttaatacc gtcaataata ctcgcatgat tttcttgatc
     3901 tgaaaacacg acacaatttg tatttttgaa aatattaaat aacgccaaat tagcatcata
     3961 accactattt aagatagtac atgcactata tccgagccaa cctgctaaca ttgtttcaat
     4021 ttcttcataa gctgtcgaac ttccactaat taatcttgaa cttgataagt gataactata
     4081 cttccgcata aatctttcga aatcatcctt atcaaacgct atttgaccta atcctaaata
     4141 atcattagat gtatagttcg tacatctctt attttctact tcaatatact gtctatctat
     4201 ataccctacc gatttaagcg accgatataa ccctttctgt tgtaataaat caatttgctc
     4261 ttgaaacatc attcttgttt tccttatttt cacaagtgtc ataatcaatt tcaaagccca
     4321 aatcattaat catatcgtag tctaattggt tcggttgccc accagtaatt agataatcac
     4381 cgacaaatat tgaattcgcc gcttttaatg ctaatggctg taacgaacgt aagttgacct
     4441 ctcttcctcc agcaatacga atttctttcg taggattgat taatcggaat aatgctacga
     4501 ttcttaaaca tttcattggt gttaaatcat ccatgcttcc aaactttgtg cctttgattg
     4561 gatgcaaaaa attaatcgga atactgtcgg catccatttc tttcaaagca aatgccatat
     4621 caacaatatc ttgattagat tctcccatac cacaaatcac gccagaacat ggtgatatat
     4681 tattcgcttt cattagttct atcgtatctg ttctatcttt ataactatgc gttgtcacga
     4741 cgttatcatg gtaattttca cttgtattaa tattgtggtt atatctgtct acaccagctg
     4801 acttaagctt cttagcttgt tcgtcattcg ttaatcctaa acatgcacag atttttagtt
     4861 gcgggtgttg agatttaatc gttcttacag tattactaat atgatcaact tctttatcgc
     4921 tcggtcctct accactcata acaatacaat atgttccaat atgattatca tgtgccacct
     4981 ttgctccatc gataatttgt tcctctggaa ttaaagcata tcgctgtttt tgtttaatat
     5041 ctcgtgattg tccacagtac ccacaattct caggacatat accactttta gcatttaaaa
     5101 tcatgtttaa ttttactttt ttaccgaaat aatgttttct taaaatgtac gcctcattta
     5161 ataaatctaa ggtatcaata ttagtatcct cataaatttt caatacagtc tcttttgtta
     5221 attgttcccc ttgtaatatg cgtttagcca aattcatatt aacacttcct atctaaaatg
     5281 ttatgtactg cctttttaaa aatacgtacc atcgttttca cttctttatt tgacatactt
     5341 aacactggca caaacgtaat gacattttct aaatttctaa tcattagccc gttttcttta
     5401 catttacgta caatacgttc aactttttca atttctaacg gcgttttact atctttatct
     5461 gtaactaatt caactccaaa cattaagcct cgtcctctta catcaccaac atttcgatga
     5521 cgcttcagcg catgcaactg tttctcaagt gttgatgatg tcgtttcaat atgtgacaat
     5581 aacttacgtt tttcataaag tcttatattt tctaatgcaa ccgtacaaac gatttgattc
     5641 ccggtgtatg tatgaccatg gaaaaaggta ttcacaccat gcgaatcact taaaaatgca
     5701 ttgtatattt tttgagatgt caatgtagct gcaagtggta agtagccacc tgtaatcgcc
     5761 ttacctaaac acataatatc cggttgaaca tcttcatgat tgcatgcaaa catctttcca
     5821 gttctcccaa aaccaactgc tacctcatca caaattaata agacatcgta ttttttgcac
     5881 aatttctcga cttctttcaa aaagccttta ggatgaacaa acaagcctgt cgcaccttga
     5941 atcaacggtt ccaatataaa ccctgcgatt tcatcatttc tttcaattag aatttgctct
     6001 atattcgtta aaatagctgt catcatttca ttttcagtat cgtaattact gcgatagaaa
     6061 gatgggcttt ctacttgaat attctcaaat attaagtctt taaatatttt atgaaaggtc
     6121 ttgataccac caacacttac cgcaccaatc gtatccccat gataaccgtg atttagcgtt
     6181 ataaacttgt ttttcttggc atatttttct ctatcaatat ttttccaata ctgatatgcc
     6241 atctttattg cgatttcaac agacgcactg cctgtatcag aataaaatac ttttcttaga
     6301 ttacttggcg tgatttcgat taatttttcc gcaagttcta ttgacggaat atttgatgat
     6361 cctagcagcg tagaatgggc aattttattg agttgctttt taattacctt attcaagtat
     6421 ttgttattat gaccatgcac attgacccac aacgatgcat aaccatctaa atatttattg
     6481 ccattcgtat cgtaaaggta actacccttt cctttttcaa tgatgattgt ttcttctttg
     6541 ctatatacac ccatttgtgt aaatggatgc caaacatatt ctgagtcttt ttgtttaagt
     6601 tgttgtgtgt aattcatttt taactcctat taattgtttt aaaaatgctt ctgagaaatt
     6661 ttcatacgtg gcatgttctt caaatgtata gactgtttta tttgttaatt ttccaatcgt
     6721 aatttgattg tctttttcaa tatagctgtc tgtatagcga ttcattatta aaaaattact
     6781 cgccgataca ttctgattaa cataatcttg gtgaacaatg gcatcgctaa tagcacctaa
     6841 ttttgatggc aacacactga tgacacaatc tgcacaatca ttgattagat ctttagtcat
     6901 gtagaaatca tctgtacctt catatattgg tacggcaatt cccccagcac cctcaattaa
     6961 gataaaatca aattccttat ctaaaacttt taccttatct aatacacgct gcttatttag
     7021 aaaaatttga tctgtcattt taaatgcaag gtgtggtgat acaggttgct taaaagtata
     7081 aagtgacgtt atgtcatagc ttaaatcaca ttcattttta aatacttcta aatctggaaa
     7141 cgtcccgtct tggcgttcct cagtttgaaa tggtttaaaa atacaaactc gatgaccacg
     7201 tgttttcaaa gcatggtata aatgctttgt aacataggtt ttgcctacat cagtattcgt
     7261 acttgtaata aaaatcctca taaaaaataa gcacctcaat ccgaatgtaa acttttataa
     7321 ttaataagtt tacatttaag gcgcatgttt ttcaagaaaa catgaattta aacttctcag
     7381 tataactata aattttggca aaattttgac cttgctattg aaatgactaa gacttcttaa
     7441 ggaattcggg cagatataaa cagcgattcg ataaaatatg tcagttaaaa ttaataattg
     7501 cattatttct taacaaaaag acatcctagc tttaccatct tgtgaaacaa tccattaatt
     7561 gtaatattac gttgagcgcc ttgttcatca atgctataat tagttacata ataaattgaa
     7621 catctaaata caccaaatcc cctcactatt gccctagtga ggggatttat tttgctgttg
     7681 attatctatc acccttttta tttccaaatt ctgattccat attaaactat tatttataat
     7741 ttccagcctg ctgatttact ttttattctt ataaagtctt gatacattcc tggttttcga
     7801 attaattcat catgactacc tttttgaatt atttcacctt cattgagcac tataatctga
     7861 tctgcatttt taatagtttc aagtttatgt gcaattgtaa ttactgtttt gcctttactt
     7921 aattcattaa ttgccgtttg aatcagctgt tcattttcag ggtcaatact tgcagttgct
     7981 tcatctaaaa taattattgg tgcatctttt aatatcgccc tagcaataga aatcctttgc
     8041 ttttctccgc cagataaatt actacctttt tcatttagca ttgtttgata tccctcaggt
     8101 aatgacatga tgaagtcgtg gcaacatgct tgcttcgctg cacgaataat ttcttctttc
     8161 gttgcacctg gattgccaaa caatatgtta ttttcaatcg tatcattaaa taaatacacc
     8221 ttttgaaata ctgcactaat tttcgacatt aacgtactta atgtcatatc tttaatatca
     8281 acaccatcga tgcgaatatt tccatcatcg atatcataaa agcgcaagag taagtgacat
     8341 aatgtagatt tcccacttcc tgaaggtcca attattgctg ttgatgtttg tgtaggtatc
     8401 tcaaaattca cattctttat cacttgttta tcatcatatg aaaagttcac gttttgaaaa
     8461 gcgatatttt catttttaat cgtcaaattt tttccattct catccagttc tggagcattt
     8521 tttatctttt caatatcatc tatcgtcata tctatcattt caagtacgtg tgcagcacta
     8581 ttaatatttt caacactatc aaatatcaca aatgaaaaaa ttgaaatcat aataagggta
     8641 ggtaaatcaa tactatgatt catatataat aagcatgcaa ctaatacaat cattattgaa
     8701 acaactttta aactaagtaa atgcaataaa ttgaatggga tatattgcat ttctattttt
     8761 gtatttatac gtttactttc attgactgct tggttaaaac ttttaagact cgtattttct
     8821 ttcgcgaatg attttattac ttgaatacca cgaataacct ccaatacctt ttccactaat
     8881 tggttttgta cattatgata cgctggcgca ttttgtcgac tctttctttc taataattga
     8941 atcgcaaaaa atgatagtaa tacgccaatg catgctaata atgatacttg ccacgaaact
     9001 acaagtagag acaatatgag tactgtaatt aatatgtatc cattaacaac aacgtccacc
     9061 attttcatag caaagttttc taaaaaggtt aaatctgttg ttactattgt tgttaactca
     9121 tttgaatgat gcgaattaaa ataacctaac cttacatttt tcaatttatc ccctatatct
     9181 aaacgttctt tcgcactcat ttcataagcg atgctctcat ggcttttgct tttgaaatat
     9241 gctgtaataa atcgtccaat cactaataaa accatgataa ttacaacatt caatatatct
     9301 ttcatataaa taggtttatg agatagcaca ttattaaata tttttgcagc taaaaagata
     9361 ggtagcgcaa taaaaatagc atttaaaaat gacatgctaa atcctaaaat cattcttgct
     9421 ttatatggtc ttatccagtt taaaatttta aatgtaattt gaaacatatt tgacctcctt
     9481 ttctcattag tttaattatg tccagtattg attccccaat cttttgtatg catgtgcgta
     9541 tcccacattt tcttataatt accgtttaat tttagcaata agtgatgtga ccctttttct
     9601 aaaatttgtt gtttacctaa aacaataatt tgatctgcat gttgaatcgt agataaccga
     9661 tgtgcaataa caatcaatgt tttatcctgc gtcaacacat ttaatgcttc ttgaattttt
     9721 tgttcgttgt ctggatcaac gtaagcagtc gcttcatcta atacaatgat aggcgcatct
     9781 tttaatatca ttcttgcaat agtgactcgt tgtttttcac caccagacaa tttatctccc
     9841 actgtaccaa cattcgtatc atatccatct ggcaactttt caataaattc atgacattgt
     9901 gctaacttgg cagctttttc aactgcctca tccgtagctt ctggattgcc aagtttaata
     9961 ttttctttaa aagtaagatt taataaaaag ttatcttgtc caacaaaacc aactaaatcg
    10021 ttaagttgtt tcgattcaat atcttttata tttataccgc caatcgtaat ttcacctgaa
    10081 gtcacatccc agtatcgtga tataagcttg gcaatggttg acttaccgct accagatgcc
    10141 ccgacgatag ctgtgaaatt attttctggt actgtaaatg ataaatgctt aaagaccaga
    10201 tcgtccttat ctttgttata tgaaaatcca acattattaa aagcaatttc ataatgttga
    10261 ggcttcttaa attttgttga caatactaac tcttctagac ttaatatttg attcacttca
    10321 gttaatgcgt attgtataga ctttaaatga tttacataat tagtaaaatt cttaatcggt
    10381 gctactacac ctaaagataa tacgatgcat aggaaaaatt ccgcatagtt taattggttg
    10441 atagatatca aatacatgcc gaccggtaaa atccctaaaa atgttgaagg taatacacta
    10501 gctcctaaat tcatataccc ccatgtattt ttaaaccaat tcaaagtgtg aatcttataa
    10561 ttatctactg catctttata ttttttatat gaactttgag attgattaaa tgttttaatc
    10621 acttcaatgc cttcgataaa ctctacaatc gcgctattca tataattatt cgatttcatt
    10681 tgttcagcat atgtctcatt aaatccagac ataacttttt taaaagcgaa aattgaaatt
    10741 ggtatcgtta ctaataaggc actagccata cgccaatcaa tgagcattat gtataaaaag
    10801 atagcagctg acaaaagtaa gtttcctata acttcaggaa tcatatgtgc taaaggtaat
    10861 tctattgttt caaccttatc gacaaatata ttttttaatt cacctatttt cttagattcc
    10921 actacgccta aagggagacg cattaatttt tgagctaatt ttttacgaat ttcagataaa
    10981 atttcatatg ccgtaatatg tgatagcatc gttgacgctc caaaacaaca cacttgtgaa
    11041 atataagcga ttaaagcaat aaagatataa accataatcg aattaatcgt atatgcattg
    11101 ttaatcatca ttaaaataat tttaaagact gcccaatatg gaactaatcc agaaaagaca
    11161 ctgatgatag acaacaaaat tgataacata attttccatt tactatcaga agcaaatccg
    11221 ctgagtatct ttatccaatt tgtctgattg ttcatcgtat atgtaactcc tttcaattaa
    11281 taatctaaat taagccgctt atattattta tttcactg
//
LOCUS       DBNENW010000084        11206 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNENW010000084 DBNENW010000000
VERSION     DBNENW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.084"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            24..2729
                     /gene="acnA"
                     /locus_tag="AC1LW8_001747"
     CDS             24..2729
                     /gene="acnA"
                     /locus_tag="AC1LW8_001747"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIP4773576.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2909..3376
                     /gene="menI"
                     /locus_tag="AC1LW8_001748"
     CDS             2909..3376
                     /gene="menI"
                     /locus_tag="AC1LW8_001748"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase MenI"
                     /protein_id="HIP4773577.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDDIDSL"
     gene            complement(3583..3879)
                     /locus_tag="AC1LW8_001749"
     CDS             complement(3583..3879)
                     /locus_tag="AC1LW8_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HIP4773578.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4254..4862)
                     /gene="plsY"
                     /locus_tag="AC1LW8_001750"
     CDS             complement(4254..4862)
                     /gene="plsY"
                     /locus_tag="AC1LW8_001750"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIP4773579.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5068..7059
                     /gene="parE"
                     /locus_tag="AC1LW8_001751"
     CDS             5068..7059
                     /gene="parE"
                     /locus_tag="AC1LW8_001751"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIP4773580.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7059..9461
                     /gene="parC"
                     /locus_tag="AC1LW8_001752"
     CDS             7059..9461
                     /gene="parC"
                     /locus_tag="AC1LW8_001752"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIP4773581.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYGAMVRLSQGWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9711..11171
                     /locus_tag="AC1LW8_001753"
     CDS             9711..11171
                     /locus_tag="AC1LW8_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIP4773582.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         3898 a         1621 c         2167 g         3520 t
ORIGIN      
        1 aatgtatcaa gggggatcat taaatggctg caaattttaa agagcaatca aaaaaacatt
       61 ttgacttgaa tggccaaagt tatacttact atgatttaaa agctgtagaa gagcaaggta
      121 ttactaaagt ttccaattta ccttattcaa ttcgtgtttt gttagaatct ttacttcgtc
      181 aagaagatga ttttgtaatt acagacgatc atattaaagc tttaagtcag tttggaaaag
      241 atggaaatga aggcgaggta ccatttaaac cttctcgtgt tattttacaa gatttcacag
      301 gtgtaccagc cgtagttgat ttagcttctt tacgtaaagc aatggatgac gttgggggag
      361 atattactaa aattaatcca gaagtaccgg tggatttagt tattgaccac tcagttcaag
      421 tggatagcta tgcaaatcca gaagctcttg aacgtaatat gaaattagaa tttgaacgta
      481 actatgaacg ttatcagttt ttaaattggg caacgaaagc atttgataat tacaatgcag
      541 ttcctcctgc aactggaata gttcaccaag ttaacttaga atatttagca agtgttgtac
      601 atgttcgtga tgtagatggt gaaaaaactg catttccaga tacattagtt ggtactgatt
      661 cacatacaac aatgataaat ggtattggcg tactaggatg gggtgttggt ggtattgaag
      721 ctgaagctgg aatgcttgga caaccttctt atttcccaat tccagaggtt attggtgtac
      781 gactagtaaa ttcattacca caaggcgcaa cagcaactga tttagcgtta agagtaactc
      841 aagagctacg taaaaaaggt gttgttggta aatttgtgga gttctttggt ccaggtgtac
      901 aacatttacc actagcagac cgtgctacaa ttgcaaacat ggcaccagag tatggagcaa
      961 cttgcggatt cttcccagtt gatgatgaat ctcttaaata tatgaagtta actggtagat
     1021 cagacgaaca tatcgcgcta gtaaaagaat atttgaaaca aaaccatatg ttctttgatg
     1081 ttgagaaaga agatcctaat tatacagatg ttatcgaatt ggatttatca acagttgaag
     1141 catcgctttc aggaccaaaa cgtcctcaag atttaatttt cttaagtgat atgaaatcat
     1201 catttgaaaa ttctgtaaca gctccagcag gcaaccaagg acacggttta gataaaagtg
     1261 aatttgataa gaaagctgaa attaacttta aagatggatc aaaagctaca atgaaaacag
     1321 gtgatattgc aatagcagca attacatcat gtacaaatac atctaaccct tatgtaatgt
     1381 taggtgcagg tttagttgct aaaaaagcag ttgaaaaagg cttgaaagtt cctgaatacg
     1441 ttaaaacttc tctagcacca ggatcaaaag ttgttaccgg atatttaaga gatgctggct
     1501 tacaacctta tttagatgat ttaggcttca acttggttgg ttatggatgt acaacttgta
     1561 tcggtaattc aggtccttta ttaccagaaa ttgaaaaagc gattgctgat gaggacctat
     1621 tagtgacatc tgtattatct ggtaaccgta actttgaagg tcgtatccat cctcttgtta
     1681 aagccaatta cctagcttca ccacagttag ttgttgctta tgcattagct ggaacggttg
     1741 atattgattt acagaatgaa cctattggta aaggtaatga cggtgaagat gtatatttga
     1801 aagatatttg gccatcaatt aaagaagttt cagataccgt tgatagtgtt gtaacacctg
     1861 aattatttat tgaagaatat aataacgtat acaataacaa cgaattatgg aatgagattg
     1921 atgtaactga tcaacctcta tatgactttg atcctaattc aacatacatt caaaatccat
     1981 cattcttcca aggattatct aaagaaccgg gtacgattgt tccattaaat ggtttacgtg
     2041 ttatgggtaa attcggtgat tctgtgacaa ctgaccacat ctctccagca ggtgcaattg
     2101 gtaaagatac gccagctggt aaatatttac aagatcatca agtgcctatt cgtgaattta
     2161 attcatatgg ttcaagacgt ggtaatcacg aagtaatggt tcgaggtacg tttgctaata
     2221 tacgtattaa aaaccaatta gcgccaggta ctgaaggtgg ttttacaact tattggccaa
     2281 caaatgaagt aatgcctatc tttgatgctg caatgaaata taaagaagat ggtacaggtt
     2341 tagttgtatt agctggtaac gattatggta tgggttcatc tcgtgactgg gcagcaaaag
     2401 gtacaaactt attaggtgtt aaaacagtta ttgcacaaag ttatgaacgt atccatcgtt
     2461 caaatttagt tatgatgggt gtattaccat tagagtttaa aaaaggtgaa tcagctgatt
     2521 ctcttggtct agatggtaca gaagaaattt ctgttaatat tgatgaaaat gttcaaccac
     2581 atgactacgt caaagttact gctaagaagc aagatggtga tttggtagaa tttgacgcta
     2641 tggttcgttt tgactcactt gttgaaatgg attactatcg tcacggtgga attttacaaa
     2701 tggttttaag aaataaatta gcgcaataaa aaatagatat cacagtaaaa ttttaatcgg
     2761 tatttgaggc actttgatag tgctcaaaaa tacttctata aactttatga tataataggt
     2821 cggttcaaaa cctttgtgtt cttggccggc ctcttcttgt attttatgtt agaatgagat
     2881 ataaagttaa actgaaagaa ggcttaaaat gatatatagt attacagaaa tagaagcgcg
     2941 ttatgctgaa actgataaga tgggtgtaat ttatcacggg aattatgcaa cttggtttga
     3001 agttgcgcgg ttggattata tatcgaagtt aggttttagt tatgctgata tggaaaaaca
     3061 aggaatcatt tcacctgtga ctgacctcaa tgtcaattat aaaaagtcta ttttttatcc
     3121 agaaaaagtt aaagttaaaa cttgggttga aaaatattcg agattacgtt cagtgtataa
     3181 atatgagatt tttaatgaaa aaggtgaact tgcaactaca ggttccacag aattgatttg
     3241 cattaaagaa gatactttta agcctatacg gttggatcgt tatttcccag attggcatga
     3301 agcttatagt aaagtgcaaa cgctcaataa tgaagggaaa acagtagaga taatggatga
     3361 tattgattct ttataagtta ctgtaaatgt ttggagaggc aatattaaca attaatctat
     3421 aaaaaatagc caatacgtta acattaatgt aattagcgtg aagataagct aatatatagc
     3481 aaaaacgaac ttgttcatac aacatcagat gtgctaaacc aaagtacaac ttccgaggtt
     3541 caggacacat cagaatgtgt aaacaagttc gttttatttt agttattttg tggaataaga
     3601 aatttcatct tcgtgatcaa ctacatttac aataatgtgg tcatcttcaa agtaccacaa
     3661 atctttttcc gctacgacaa catttaaatc gtcatattgt tgttcatagc caatatcaac
     3721 atcttccttt ggttcaactg taaaagcagg actaaatcct tgcttgagtt ggaattcgcc
     3781 accatatctt acaaaaaaca cgagcacttt attattttca ggcaactcaa gttcattttt
     3841 aaaccaagtt actgctgcat cagtaagttc tatttgcata acaatgacct cctatattta
     3901 ttacagaatg ggagtgggcc aacttgtatt atttgtggaa tttcttatcg aaattctctg
     3961 tgttggagcc caccccaacc tgctcattat tgtaagctga atttccataa gcttctatgt
     4021 tgggaccccc gaccagaatt gaaaaaacct agttataagc gcattttgtt cggttaacta
     4081 cagctaatat aacattgtgg agcctatgac attgatttat gtctcggtct caaatgttct
     4141 tgtaaaaaac taagtatagt tgaattttaa gttaaaaaaa ttgtttttgc aaaaataaaa
     4201 taatccatca actgcaaaaa aatatgcaat gatggactaa tgtcaaaaaa ttgttacatc
     4261 cattttattt taggttcttc gcctctaaaa atccttgcga tattagagcg atgtctaatt
     4321 atcaatatga ttgaaactaa gaaactaacg actaataaaa tatagtcttg aatgataagc
     4381 gagccaatca cacagcaaat tgctgcaacg atacttgcta aagaaacata tttaaaaatc
     4441 ttcaatacaa taaagaagat aattgcaagt attagtaaaa gtatcggatt gactcccaag
     4501 acgacacctg cactagttgc aacagctttg ccaccttgga attttaaata aacaggataa
     4561 acgtgtccaa gtatagcgaa taagccaaca attaaaccat ttgtaaaaaa agtactaata
     4621 gggccatctg cgtgaactgg taaccataaa gggaagaaaa cagttatgaa ccctttgaaa
     4681 atatctagaa atgttaccaa gaatcctgca ggacgaccta atactctaaa gctattagta
     4741 gcgccagtat taccactacc aaattgtcta atatcttttt tgaaaaataa ttttccaatt
     4801 acgaatccac ttgggaaagc gccgataaga taacttagta gtaacatgac gattatcatc
     4861 ataaatatta cacatccttt aatatcttag gactatttta tcatattcgt ttaaattacg
     4921 gctaaaaatt ttaaaaacgg ggattaatat atggaattaa gctatgaaag ttaattgata
     4981 cttgcatttt acgctgattt atataagaat aactattgta tagttttaaa aacgaacgta
     5041 cgtttgcagg aggcgaaatc attggcaatg aataaacaaa ataattattc agatgattca
     5101 atacaggttt tagaggggtt agaagcagtt cgtaaaagac ctggtatgta tattggatca
     5161 actgataaac ggggattaca tcatctagta tatgaaattg tcgataactc cgtcgatgaa
     5221 gtattgaatg gttacggtaa cgaaatagat gtaacaatta ataaagatgg tagtatttct
     5281 atagaagata atggacgtgg tatgccaaca ggtatacata aatcaggtaa accgacagtc
     5341 gaagttatct ttactgtttt acatgcagga ggtaaatttg gacaaggcgg ctataaaact
     5401 tcaggtggtc ttcacggtgt tggtgcttca gttgtaaatg cattgagtga atggcttgaa
     5461 gttgaaatcc atcgagatgg taatatatat catcaaagtt ttaaaaacgg tggttcgcca
     5521 tcttctggtt tagtgaaaaa aggtaaaact aagaaaacag gtaccaaagt aacatttaaa
     5581 cctgatgaca caatttttaa agcatctaca tcatttaatt ttgatgtttt aagcgaacga
     5641 ctacaagagt ctgcgttctt attgaaaaat ttaaaaataa cgcttaatga tttacgcagt
     5701 ggtaaagagc gtcaagagca ttaccattat gaagaaggaa tcaaagagtt tgttagttat
     5761 gtcaatgaag gaaaagaagt tttgcatgac gtggctacat tttcaggtga agcaaatggt
     5821 atagaggtag acgtagcttt ccaatataat gatcaatatt cagaaagtat tttaagtttt
     5881 gtaaataatg tacgtactaa agatggtggt acacatgaag ttggttttaa aacagcaatg
     5941 acacgtgtat ttaatgatta tgcacgtcgt attaatgaac ttaaaacaaa agataaaaac
     6001 ttagatggta atgatattcg tgaaggttta acagctgttg tgtctgtacg tattccagaa
     6061 gaattattac aatttgaagg acaaacgaaa tctaaattgg gtacttctga agctagaagt
     6121 gctgttgatt cagttgttgc agacaaattg ccattctatt tagaagaaaa aggacaattg
     6181 tctaaatcac ttgtgaaaaa agcgattaaa gcacaacaag caagggaagc tgcacgtaaa
     6241 gctcgtgaag atgctcgttc aggtaagaaa aacaagcgta aagacacttt gctatctggt
     6301 aaattaacac ctgcacaaag taaaaacact gaaaaaaatg aattgtattt agtcgaaggt
     6361 gattctgcgg gaggttcagc aaaacttgga cgagaccgca aattccaagc gatattacca
     6421 ttacgtggta aggtaattaa tacagagaaa gcacgtctag aagatatttt taaaaatgaa
     6481 gaaattaata caattatcca cacaatcggg gcaggcgttg gtactgactt taaaattgaa
     6541 gatagtaatt ataatcgtgt aattattatg actgatgctg atactgatgg tgcgcatatt
     6601 caagtgctat tgttaacatt cttcttcaaa tatatgaaac cgcttgttca agcaggtcgt
     6661 gtatttattg ctttacctcc actttataaa ttggaaaaag gtaaaggcaa aacaaagcga
     6721 gttgaatacg cttggacaga cgaagagctt aataaattac aaaaagaact tggtaaaggc
     6781 ttcacgttac aacgttacaa aggtttgggt gagatgaacc ctgaacaatt atgggaaacg
     6841 acgatgaacc cagaaacacg aactttaatt cgtgtacaag ttgaagatga agtgcgttca
     6901 tctaaacgtg taacaacatt aatgggtgac aaagtacaac ctagacgtga atggattgaa
     6961 aagcatgttg agtttggtat gcaagaggac caaagtattt tagataattc tgaagtacaa
     7021 gtgcttgaaa atgatcaatt tgatgaggag gaaatctagt gagtgaaata attcaagatt
     7081 tatcacttga agatgtttta ggtgatcgct ttggaagata tagtaaatat attattcaag
     7141 agcgtgcatt gccagatgtt cgtgatggtt taaaaccagt acaacgtcgt attttatatg
     7201 caatgtattc aagtggtaat acacacgata aaaatttccg taaaagtgcg aaaacagtcg
     7261 gtgatgttat tggtcaatat catccacatg gagacttctc agtgtacgga gcaatggtcc
     7321 gtttaagtca aggctggaag ttacgacatg tcttaataga aatgcatggt aataatggta
     7381 gtatcgataa tgatccgcca gcggcaatgc gttacactga agctaagtta agcttactag
     7441 ctgaagagtt attacgtgat attaataaag agacagtttc tttcattcca aactatgatg
     7501 atacgacact cgaaccaatg gtattgccat caagatttcc taacttacta gtgaatggtt
     7561 ctacaggtat atctgcaggt tacgcgacag atataccacc acataattta gctgaagtga
     7621 ttcaagcaac acttaaatat attgataatc cggatattac agtcaatcaa ttaatgaaat
     7681 atattaaagg tcctgatttt ccaactggtg gtattattca aggtattgat ggtattaaaa
     7741 aagcttatga atcaggtaaa ggtagaatta tagttcgttc taaagttgaa gaagaaactt
     7801 tacgcaatgg acgtaaacag ttaattatta ctgaaattcc atatgaagtg aacaaaagta
     7861 gcttagtaaa acgtatcgat gaattacgtg ctgacaaaaa agtcgatggt atcgttgaag
     7921 tacgtgatga aactgataga actggtttac gaatagcaat tgaattgaaa aaagatgtga
     7981 acagtgaatc aatcaaaaat tatctttata aaaactctga tttacagatt tcatataatt
     8041 tcaacatggt cgctattagt gatggtcgtc caaaattgat gggtattcgt caaattatag
     8101 atagttattt gaatcaccaa attgaggttg ttgcaaatag aacgaagttt gaattagata
     8161 atgcagaaaa acgtatgcat atcgttgaag gtttgattaa agcgttgtca attttagata
     8221 aagtaatcga attgattcgt agctctaaaa acaagcgtga cgctaaagaa aaccttatcg
     8281 aagtatacga gttcacagaa gaacaggctg aagcaattgt aatgttacag ttatatcgtt
     8341 taacaaacac tgatatagtt gcgcttgaag gtgaacataa agaacttgaa gcattaatca
     8401 aacaattacg tcatattctt gataaccatg atgcattatt gaatgtcata aaagaagaat
     8461 tgaatgaaat taaaaagaaa ttcaaatctg aacgactgtc tttaattgaa gcagaaattg
     8521 aagaaattaa aattgacaaa gaagttatgg tgcctagtga agaagttatt ttaagtatga
     8581 cacgtcatgg atatattaaa cgtacttcta ttcgtagctt taatgctagc ggtgttgaag
     8641 atattggttt aaaagatggt gacagtttac ttaaacatca agaagtaaat acgcaagata
     8701 ccgtactagt atttacaaat aaaggtcgtt atctatttat accggttcat aaattagcag
     8761 atattcgttg gaaagaattg ggacaacatg tatcacaaat agttcctatc gaagaagatg
     8821 aagtggttat taatgtcttt aatgaaaagg actttaatac agatgcattt tatgtttttg
     8881 cgactcaaaa tggcatgatt aagaaaagta cagtgcctct atttaaaaca acgcgtttta
     8941 ataaaccttt aattgctact aaagttaaag aaaatgatga tttgattagt gttatgcgct
     9001 ttgaaaaaga tcaattaatt accgtcatta ctaataaagg tatgtcatta acgtataata
     9061 caagtgaact atcagatacc ggattaaggg cagctggtgt taaatcaata aatcttaaag
     9121 ctgaagattt cgttgttatg acagaaggtg tttctgaaaa tgatactata ttgatggcca
     9181 cacaacgcgg ctcgttaaaa cgtattagtt ttaaaatctt acaagttgct aaaagagcac
     9241 aacgtggaat aactttatta aaagaattaa agaaaaatcc acatcgtatt gtagctgcac
     9301 atgtagtgac aggtgaacat agtcaatata cattatattc aaaatcaaat gaagaacatg
     9361 gtttaattaa tgatattcat aaatctgaac aatatacaaa tggctcattc attgtagata
     9421 cagatgattt tggtgaagta atagacatgt atattagcta aaaactatat gcaatcacga
     9481 aattaaatga taaaatacag taatgttaaa ttttgactaa attcaaggga tttatattaa
     9541 atgctgacca agtacttatc gttaaattag cgatatactt tgaccaaact aatataaatc
     9601 tcttgtattg tataagaaat gatgatgtat ctctatattt actgaagttt caattcggtc
     9661 taattttata gaaggtacat gattcatgta gaggatgcga ggggtttatt ttgaaagatt
     9721 tcgatagttt aattcctgga tggtttaaag aatttgtcca tgttggtacc gatttaatat
     9781 ggtctcaata tttaattggt ctattattga cagctggatt cttctttaca attagttcta
     9841 aattcgtcca attacgaatg ttacctgaaa tgtttagagc tttagtagaa cgtccagaaa
     9901 ctttagaaga tggtaagaag ggtatttcgc cattccaagc atttgcgatt agtgctggtt
     9961 cgagagttgg tactggtaat attgctggtg ttgcgactgc gattgtttta ggcggtccag
    10021 gtgcagtgtt ttggatgtgg gttattgcat ttataggtgc agcgagtgca tttatagaag
    10081 cgactttggc tcaggtttat aaagtacatg ataaagatgg tggattccgt ggtggtccag
    10141 cttactatat tactaaaggt ttaaatcaaa aatggctagg tatcgtattt gcgattttaa
    10201 ttacaattac atttgcattt gtatttaaca cagtgcaatc taatacaatt gcggagtcgt
    10261 taaatacgca atataatatt agtccagtaa tcacaggtat tattttagca atcgtaacag
    10321 ctattattat atttggtggt gtacgtagta ttgctacgtt atcttcgtta attgtaccga
    10381 ttatggctat catttacatt ggtatggttt tagtaatatt gctatttaat ttagatcaaa
    10441 ttgttcctat gataggtacg attattaaaa gtgcatttgg tatcgaacaa gtaactggtg
    10501 gcgctgtagg tgctgcggtt cttcaaggta tcaaacgtgg tttattctct aacgaagctg
    10561 gtatgggttc tgcgccgaat gcagcggcaa ctgctgccgt accacaccct gttaagcaag
    10621 gtttaatcca atcattaggt gtgttctttg atacaatgtt ggtttgtaca gcaactgcaa
    10681 tcatgatttt actatattca ggactgaaat ttggtgataa cgcacctcaa ggtgttgcag
    10741 ttactcaatc agcacttaat gagcatttag gttctgctgg aggtattttc ttaacaatag
    10801 cagttacact gtttgcattt tcatctgtgg taggtaatta ctattacggt caatctaata
    10861 ttgaattttt atcaacaaac cgtgtaatat tatttatctt tagatgtctt gttgtagtac
    10921 ttgtctttgt cggtgcagtt gtaaaaacag aaacagtatg gaatacggca gacttattta
    10981 tgggcttaat ggcaattgta aacattattt ccattatagg actgtccaat gtagcttttg
    11041 cattgatgaa agattatcaa aagcagaaaa aagaaggcaa gaaccctgtc tttaaacctg
    11101 aaaacttaga aattaactta tttggaatta gtgcttgggg cgctaacaaa tataagaact
    11161 ctgataaata aatattttta atgtaacgat tcattatata aaaatc
//
LOCUS       DBNENW010000085        11198 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNENW010000085 DBNENW010000000
VERSION     DBNENW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.085"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            46..1734
                     /gene="ilvD"
                     /locus_tag="AC1LW8_001754"
     CDS             46..1734
                     /gene="ilvD"
                     /locus_tag="AC1LW8_001754"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIP4773583.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1762..3531
                     /gene="ilvB"
                     /locus_tag="AC1LW8_001755"
     CDS             1762..3531
                     /gene="ilvB"
                     /locus_tag="AC1LW8_001755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIP4773584.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3531..3785
                     /locus_tag="AC1LW8_001756"
     CDS             3531..3785
                     /locus_tag="AC1LW8_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIP4773585.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            3922..4926
                     /gene="ilvC"
                     /locus_tag="AC1LW8_001757"
     CDS             3922..4926
                     /gene="ilvC"
                     /locus_tag="AC1LW8_001757"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIP4773586.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQDASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            4956..6485
                     /locus_tag="AC1LW8_001758"
     CDS             4956..6485
                     /locus_tag="AC1LW8_001758"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIP4773587.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYGIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6488..7534
                     /gene="leuB"
                     /locus_tag="AC1LW8_001759"
     CDS             6488..7534
                     /gene="leuB"
                     /locus_tag="AC1LW8_001759"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIP4773588.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7548..8918
                     /gene="leuC"
                     /locus_tag="AC1LW8_001760"
     CDS             7548..8918
                     /gene="leuC"
                     /locus_tag="AC1LW8_001760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIP4773589.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFVKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEISDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            8919..9491
                     /gene="leuD"
                     /locus_tag="AC1LW8_001761"
     CDS             8919..9491
                     /gene="leuD"
                     /locus_tag="AC1LW8_001761"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIP4773590.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9506..10774
                     /gene="ilvA"
                     /locus_tag="AC1LW8_001762"
     CDS             9506..10774
                     /gene="ilvA"
                     /locus_tag="AC1LW8_001762"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HIP4773591.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3754 a         1868 c         2170 g         3406 t
ORIGIN      
        1 actataaaac atgattaatc attaaaattt acgggggaat ttactatgcg aagcgacatg
       61 atcaaaaaag gagatcacca agcaccagca agaagtcttt tacatgccac gggcgcgcta
      121 aaaagtccaa ctgatatgaa caaaccattt gtagctattt gtaactctta tattgatatt
      181 gttcctggac atgttcactt gagagagctt gcagatatag ctaaagaagc aattagagaa
      241 gccggtgcca ttccatttga attcaataca attggtgttg atgatggaat agctatggga
      301 catatcggaa tgcgatattc tctaccatca cgtgaaatta ttgcagatgc agctgaaact
      361 gtaattaacg ctcattggtt tgacggcgta ttttacattc ctaattgtga caagattaca
      421 cccggtatga ttttagcagc catgaggaca aacgtaccag ctatcttttg ctctggtgga
      481 ccaatgaaag ctggcttatc tgcacatgga aaagcattaa cactttcatc aatgtttgaa
      541 gcagtcggcg catttaaaga aggatcgatt tctaaagaag aatttttaga tatggaacaa
      601 aatgcctgcc ctacttgtgg ttcatgtgct gggatgttta ctgcaaattc aatgaactgt
      661 ttgatggaag ttttaggtct agcattacca tacaacggta ctgcacttgc agtcagtgat
      721 cagcgacgag aaatgattcg ccaagcagct tttaaattag ttgaaaatat taaaaatgat
      781 ttaaaaccac gtgatatcgt tactcgcgaa gcaattgatg atgcatttgc acttgatatg
      841 gctatgggtg gttcaacaaa cacggtactg catacgttag ccattgccaa tgaagctggt
      901 attgattatg acttagagcg cattaatgct attgccaaac gcacgccata tttatcaaaa
      961 atagcaccta gttcatcgta ttcaatgcat gatgtgcatg aagctggtgg cgtcccagca
     1021 attattaatg aattgatgaa gaaagatggc acgttacacc cagatagaat cacagttact
     1081 ggcaaaacgt tacgtgaaaa taacgaaggc aaagaaatta agaactttga tgtcattcac
     1141 cctcttgatg caccatatga tgcacaaggc ggtttatcta tcttatttgg taatatcgcc
     1201 cctaaaggcg cagttattaa agttggcggc gttgatccat ctatcaaaac atttactggg
     1261 aaagcaattt gtttcaattc gcatgatgaa gctgttgaag caatagacaa tcgtaccgtt
     1321 cgtgcaggcc acgtcgttgt cattagatat gaaggaccta aaggtggacc aggtatgcct
     1381 gaaatgttag cacctacttc ctctattgtt ggtcgcggct taggtaaaga tgttgcatta
     1441 attactgatg ggcgtttttc cggtgccaca agaggtattg cagttggtca tatttcccct
     1501 gaagctgcat ctggtggacc aattgcctta attgaagatg gagatgagat tactattgat
     1561 ttaacaaatc gtacattaaa cgtaaaccag cctgaagatg ttctagcgcg tcgccgagaa
     1621 tctttaacac cgtttaaagc gaaagtaaaa acaggttatc tagctcgtta tactgcccta
     1681 gtaactagcg caaatacagg tggcgtcatg caagtccctg agaatttaat ttaatttatt
     1741 tttatattgg agatggttaa aatgtctaaa actcaacatg aagtaaacca aaatattgac
     1801 cctttaaaaa tggctgaatc acttgaactt gaacaactaa atgaaaaaac tttaaatgac
     1861 atgcgttcag gatcagaagt gctagtagaa gctctactta aagaaaatgt ggattattta
     1921 ttcggttatc ctggtggtgc cgtactacct ttatacgaca cattttatga tggtaaaatc
     1981 aaacatattt tagcaagaca cgaacaaggt gctgttcatg ctgcagaagg ttatgcacgt
     2041 gtatctggta aaactggcgt cgttgtagtt acaagcggtc caggtgcaac taatgtaatg
     2101 acaggtatta cggatgcaca ttgcgactct ttacctctag ttgtattcac tggacaagtt
     2161 gctacaccag gcattggtaa agatgcattc caagaagcgg atattctatc tatgacttca
     2221 ccaattacaa aacaaaatta tcaagtgaaa cgtgttgaag atatccctaa aatcgtacac
     2281 gaagctttcc atgtagctaa ttctggacgc aaaggtcctg tagtgattga ttttccaaaa
     2341 gatatgggtg ttttagctac aaatgtggat ttatgcgacg aaatcaatat tccaggttat
     2401 gaagttgtta cagaaccaga aaataaagac attgacactt tcatctcact tttaaaagaa
     2461 gcgaaaaagc ctgtcgtatt agccggcgca ggtattaatc aatcaaaatc aaatcaatta
     2521 ttaacacagt ttgttaataa acatcaaatc ccaacagtta ctactttact aggtctaggt
     2581 gctgtacctt atgaggatac actattttta ggtatgggag gtatgcatgg ttcttatgct
     2641 agtaacatgg cattaactga gtgtgattta ctcattaatt taggtagccg cttcgatgat
     2701 agattagcaa gcaaacctga tgcctttgca cctaacgcca aaattgtaca tgtagatatt
     2761 gatccttcag aaatcaataa agttattcat gtagatttag gtattattgc agactgtaaa
     2821 agatttttag aatgtttaaa tgataaaaat gttgagacta tagaacacag tgactgggtt
     2881 aaacattgtc aaaataataa gcagaaacac ccatttaaac ttggtgaaga agatcaagta
     2941 ttttgtaagc cacaacaaac aatcgaatat atcggcaaaa ttacaaatgg tgaagcaatt
     3001 gttactacag acgtgggaca acatcaaatg tgggcagctc aattttatcc atttaaaaat
     3061 cacggacaat gggttacaag cggtggttta ggaacaatgg gattcggtat tccttcgtca
     3121 attggtgcca aattagctaa tcctgataaa acagtcgtat gtttcgtcgg tgacggtggt
     3181 ttccaaatga caaaccaaga aatggcactt ttacccgaat atggtttaga tgtcaaaatc
     3241 gtactaatca ataatggaac attaggtatg gttaaacaat ggcaagataa gttctttaat
     3301 caacgcttct cacactcagt atttaatggt caacctgatt ttatgaaaat ggcagaagca
     3361 tatggcgtca aaggtttctt aatcgataag ccagaacaac tggaagaaca attagatgca
     3421 gcgtttgctt atcaaggacc agctttaatt gaggttcgta tttcccctac tgaagctgta
     3481 accccaatgg ttccgagtgg caaatcaaat catgaaatgg agggcttata atgacaagaa
     3541 ttcttaaatt acaagttgcg gatcaagtca gcacgctaaa tcgaattaca agtgcttttg
     3601 ttcgcctaca atataatatc gatacattac atgttacaca ttctgaacaa cctgggattt
     3661 ctaacatgga aattcaagtc gatattcaag atgatacatc acttcatata ttaattaaaa
     3721 aattaaaaca acaaattaat gttttaacgg ttgaatgcta cgaccttgtt gataacgaag
     3781 cttaatttta agacaaaggc aatgatgcgc taattagtta tagataaatc ataggctgct
     3841 agttaacatc tgccactatt acaaagttat atttcagaat tttcgaaaaa caaaatattt
     3901 aattatttgg aggaatttat tatgacaaca gtttattatg atcaagatgt aaaaacggac
     3961 gctttacaag gcaaaaaaat tgcagtagta ggttatggat cacaaggtca cgcgcatgca
     4021 caaaacttaa aagacaatgg atatgatgta gtcatcggca ttcgcccagg tcgttctttt
     4081 gacaaagcta aagaagatgg atttgatgtg ttccctgttg cagaagcagt taagcaagct
     4141 gatgtaatta tggtgctatt acctgatgaa attcaaggtg atgtatacaa aaacgaaatt
     4201 gaaccaaatt tagaaaaaca taatgcgctt gcatttgctc atggctttaa cattcatttt
     4261 ggtgttattc aaccaccagc tgatgttgat gtatttttag tagctcctaa aggaccgggt
     4321 catttagtta gacgtacatt tgttgaaggt tctgctgtac catcactatt tggtattcaa
     4381 caagacgctt caggtcaagc acgtaatatt gctttaagtt atgcaaaagg tattggtgca
     4441 actcgtgcag gtgttattga aacaacattt aaagaagaaa ctgagacaga tttatttggt
     4501 gaacaagcag tactttgcgg tggtgtatcg aaattaattc aaagtggctt tgaaacatta
     4561 gtagaagcgg gttatcaacc agaattagct tattttgaag tattacatga aatgaaatta
     4621 atcgttgatt tgatgtatga aggcggtatg gaaaatgtac gttactcaat ttcaaatact
     4681 gctgaatttg gtgactatgt ttcaggacca cgtgttatca caccagatgt taaagaaaat
     4741 atgaaagctg tattaactga tatccaaaat ggtaacttca gtaatcgctt tatcgaagac
     4801 aataaaaatg gattcaaaga attttataaa ttacgcgaag aacaacatgg tcatcaaatt
     4861 gaaaaagttg gtcgtgaatt acgcgaaatg atgcctttta ttaaatctaa aagcattgaa
     4921 aaataagata gacctacaat gaggagttgt taaatatgag tagtcatatt caaatttttg
     4981 atacgacact aagagacggt gaacaaacac caggagtgaa ttttactttt gatgaacgct
     5041 tgcgtattgc attgcaatta gaaaaatggg gtgtagatgt tattgaagct ggatttcctg
     5101 cttcaagtac aggtagcttt aaatctgttc aagcaattgc acaaacatta acaacaacgg
     5161 ctgtatgtgg tttagctaga tgtaaaaaat ctgacatcga tgctgtatat gaagcaacaa
     5221 aagatgcagc gaagccggtc gtgcatgttt ttatagcaac atcacctatt catcttgaac
     5281 ataaacttaa aatgtctcaa gaagacgttt tagcatctat taaagaacat gtcacatacg
     5341 cgaaacaatt atttgacgtt gttcaatttt cacctgaaga tgcaacgcgt actgaattac
     5401 cattcttagt gaaatgtgta caaactgccg ttgacgctgg agctacagtt attaatattc
     5461 ctgatacagt cggctacagt taccatgatg aatatgcaca tattttcaaa accttaacag
     5521 aatctgtaac atcttcaaat gaaattattt atagtgctca ttgccatgac gatttaggaa
     5581 tggctgtttc aaatagttta gctgcaattg aaggcggtgc gagacgaatt gaaggcactg
     5641 taaatggtat tggtgaacga gcaggtaatg cagcacttga agaagtcgcg cttgcactat
     5701 acgttcgaaa tgatcattat ggtgctcaaa ctgctcttaa tctcgaagaa actaaaaaaa
     5761 catcggattt aatttcaaga tatgcaggta ttcgagtgcc tagaaataaa gcaattgttg
     5821 gccaaaatgc atttagtcat gaatcaggta ttcaccaaga tggcgtatta aaacatcgtg
     5881 aaacatatga aattatgaca cctcaacttg ttggtgtaag cacgactgaa cttccattag
     5941 gaaaattatc tggtaaacac gccttctcag agaagttaaa agcattaggt tatggcattg
     6001 ataaagaagc gcaaatagat ttatttaaac aattcaaggc cattgcggac aaaaagaaat
     6061 ctgtttcaga tagagatatt catgcgatta ttcaaggttc tgagcatgag catcaagcac
     6121 tttataaatt ggaaacactg caactacaat atgtctctag cggccttcaa agtgctgttg
     6181 ttgttgttaa agataaagag ggtcatattt accaggattc aagtattggt actggctcaa
     6241 tcgtagcaat ttacaatgca gttgatcgta ttttccagaa agaaacagaa ttaattgatt
     6301 atcgtattaa ttctgtcact gaaggtactg atgcccaagc agaagtacat gtaaatttat
     6361 tgattgaagg taagactgtc aatggctttg gtattgatca tgatatttta caagcctctt
     6421 gtaaagcata cgtagaagca catgctaaat ttgcagctga aaatgttgag aaggtaggta
     6481 attaattatg acttataaca ttgttgccct acctggtgat ggaatcggtc cagaaatttt
     6541 gaacggatct ctatcattgc ttgaaattat aagtaataaa tataacttta attatcaaat
     6601 agagcaccac gaatttggtg gtgcctctat tgatacattc ggcgaacctt taactgagaa
     6661 aaccttaaat gcgtgtaaaa gagcagatgc tattttactg ggtgcaatcg gtggacctaa
     6721 atggacagat cctaacaatc gaccagaaca aggattatta aaattgcgta aatccttaaa
     6781 tttatttgca aatatacgcc ccactaccgt tgtcaaaggc gctagttctt tatcaccttt
     6841 aaaggaagaa cgcgttgaag gcacagattt agttatagtc cgtgaattga caagtggtat
     6901 ttattttgga gaacctagac attttaataa tcacgaggcc ttagattctc ttacttatac
     6961 aagagaagaa atagaacgca ttgttcacgt agcatttaaa ttggccgctt caagacgagg
     7021 aaaactaaca tcagttgata aagaaaatgt attagcttct agtaaattgt ggcgcaaagt
     7081 cgtaaatgaa gtaagtcaat tatatccaga agtaacagta aatcacttac ttgttgatgc
     7141 ttgtagtatg catttaatca caaatccaaa acaatttgac gtcatcgtat gtgaaaactt
     7201 atttggcgat attttaagtg atgaagcttc agtgattcct ggttcacttg gtttatcacc
     7261 ttctgctagt tttagtaacg atggtccaag attgtatgag cctattcatg gatcagcacc
     7321 agatattgca ggtaaaaacg ttgccaatcc atttggaatg attctatctt tagcgatgtg
     7381 tttacgtgaa agcttaaatc aaccagatgc tgcagatgaa ttagaacaac atatttataa
     7441 catgattgaa catgggcaaa cgacagcaga tttaggcggc aaattgaata ctactgatat
     7501 tttcgaaatt ctatctcaaa aattgaatca ctaaggggga gatgtaaatg ggtcaaacat
     7561 tatttgacaa ggtgtggaac agacatgtgt tatacgggaa attgggcgaa ccgcaactat
     7621 tatacattga tttacacctt atacatgaag ttacttctcc tcaagcattt gaaggactta
     7681 ggcttcaaaa cagaaaatta agacgcccag atttaacatt tgcaacactc gatcacaatg
     7741 ttcctactat tgatatattc aatattaaag atgaaattgc aaacaaacaa atcacaacat
     7801 tacaaaaaaa cgccatagat tttggggtgc atatttttga tatgggttct gatgaacaag
     7861 gtattgttca catggtagga cctgagacag gacttacaca gcctggaaag acaatcgttt
     7921 gtggtgactc tcacacagca acacatggag catttggtgc tattgcattt ggaattggaa
     7981 caagtgaagt tgaacatgtt ttcgcaactc aaacgctatg gcaaacaaaa cccaaaaact
     8041 taaaaatcga tattaatggt accttaccaa caggcgtcta tgctaaggac attattctgc
     8101 atttaattaa aacgtatggt gttgactttg gtacaggcta tgctttggaa tttactggcg
     8161 aaacaattaa aaacctttca atggacggtc gaatgactat ttgtaacatg gctatcgaag
     8221 gtggtgccaa atacggcata atccaacctg atgatataac atttgaatat gttaaaggga
     8281 gaccatttgc cgataacttc gttaaatcag ttgataagtg gcgtgagcta tattctgatg
     8341 acgacgcgat atttgatcgt gtaattgaac ttgatgtttc aacattagaa ccacaagtga
     8401 catggggaac taatcctgaa atgggtgtta atttcagtga accattccct gaaatcagtg
     8461 atatcaacga tcaacgtgcg tatgattata tggggttaga accaggacaa aaagctgaag
     8521 atatcgactt agggtatgtt ttcctcggtt catgtacgaa tgctagacta tcagatttga
     8581 ttgaagctag tcatattgtt aaaggaaata aagttcaccc aaatattaca gctattgtcg
     8641 tgccaggttc tcgtacagta aaaaaagaag ctgaaaaatt aggtctagat actatcttta
     8701 aaaatgcagg atttgaatgg cgtgaaccag gatgttcaat gtgtttaggc atgaatcctg
     8761 accaagtacc tgagggcgta cattgtgcat ctacaagtaa tcgaaacttt gaaggacgac
     8821 aaggtaaagg tgcaagaaca catttagtat cccctgctat ggcagcagca gcagctattc
     8881 atggtaaatt tgtggacgta agaaaggtgg ttgtttaaat ggcagcaatc aaacctatta
     8941 caacatataa aggtaaaata gtccctctct tcaacgacaa tatcgataca gaccaaatca
     9001 ttcctaaggt acacttaaag cgtatttcaa aaagtggttt cggtccattt gcttttgatg
     9061 aatggcggta cttacctgat ggttcagata atccagattt caatcctaac aaaccacaat
     9121 ataaaggggc ttctatttta attacaggag ataattttgg atgcggttca agtcgtgaac
     9181 atgctgcttg ggctcttaag gactatggtt ttcatattat tattgcagga agtttcagtg
     9241 acatatttta tatgaattgt acaaaaaatg caatgttacc aattgtttta gaaaaaaatg
     9301 cacgtgaaca tcttgctaaa tatgttgaaa ttgaggtcga tttaccaaat caaactgtgt
     9361 catcaccaga caagcgtttc cattttgaaa ttgatgaaac ttggaaaaat aaacttgtaa
     9421 atggcttaga tgacattgca atcaccctac aatatgaatc attaatagaa aaatatgaaa
     9481 aatcacttta agggagttga atattatgac agtcaaaaca acagtttcta cgaaagatat
     9541 cgatgaagca tttttaagac ttaaagatat tgtcaaagaa acacctttac aattagacca
     9601 ttacttatct caaaagtatg attgtaaagt ttatttaaaa cgagaagatt tacaatgggt
     9661 acgttctttt aaattaagag gtgcttacaa cgctatttct gttttgtcag atgaagctaa
     9721 aagtaaaggt attacatgtg cgagtgcagg taatcatgct caaggtgttg cctatacagc
     9781 taaaaaactt aatttaaacg ctgttatctt tatgccagtc actacacctt tacaaaaggt
     9841 aaatcaagta aagttctttg gaaatagtaa cgttgaagtt gtactcactg gtgatacatt
     9901 tgatcactgt ttagctgaag ctttaactta tacaagtgaa catcaaatga actttataga
     9961 tccattcaat aatgttcata caatttctgg acaaggtacg cttgctaaag aaatgctaga
    10021 acaatcaaag actgacaatg ttaactttga ttatttattt gccgctattg gtggtggtgg
    10081 attaatttca ggtattagta cttactttaa aacctattca cctactacga aaattatagg
    10141 tgttgaacct tcaggtgcaa gtagtatgta tgaatctgtt gtcgtaaata atcaggtagt
    10201 cacattgcct aatatcgata aatttgtgga cggtgcatct gtagctagag ttggcgatat
    10261 tacatttgaa attgcaaaag aaaatgtaga tgattacgtt caagtagatg aaggtgcagt
    10321 ttgttctacg attttagaca tgtattcaaa acaagcaatt gtagcagaac ctgctggcgc
    10381 attaagtgtg agtgctcttg aaaactacaa agaccacatt aagggtaaaa ctgttgtttg
    10441 tgtcattagt ggtggtaata atgatattaa tcgtatgaag gaaattgaag aacgttcttt
    10501 attatatgaa gaaatgaaac attattttat tttaaacttc cctcaacgcc ctggtgcatt
    10561 aagagaattt gtaaatgatg ttttaggccc acaagacgat attacaaaat ttgaatactt
    10621 gaaaaaatct tctcaaaata caggtactgt cattattggt attcaactta aagatcatga
    10681 tgatttaata caactcaaac aacgtgtaaa tcatttcgat ccttccaata tttatattaa
    10741 tgaaaataag atgttatatt cattgttaat ttaacacata gtaagaaaaa cagtcataaa
    10801 ttgatttcta attgaaatca tcttatgact gctttttatt atactttaca tttctcgttt
    10861 cgtcagattc aaacgttttc acttcgccaa gccatttttc tttgtgtttg cttttatttt
    10921 gacgttttag acataaaaaa agagaccttg cggtctcaac ttgcctggta acgttctact
    10981 ctagcggaac gtaagtttgc taccatcgtc gctaaagacc tttcttgact tgtgacaatc
    11041 gcttgcttct ttcctctcct tcggctctcg cttactcatt tagctctact aaactcgttg
    11101 cgctcttttc ttgtttcgtc agattcaaac gttttcactt cgccaagcca tctttctttg
    11161 tgtttgcttt ttactatgta actttagaca taaaaaaa
//
LOCUS       DBNENW010000086        11136 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNENW010000086 DBNENW010000000
VERSION     DBNENW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.086"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..410
                     /locus_tag="AC1LW8_001763"
     CDS             <1..410
                     /locus_tag="AC1LW8_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIP4773592.1"
                     /translation="LGKMIVAQDLNLQEIFFHNLIYFFSAIFGFLTLGLLNIGMLLIN
                     SGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIASTFSLGFLSYLYQRMVKKNK
                     IKNKMFHNFVTTIITVIVLTIIASLLEVYVTIN"
     gene            422..1144
                     /locus_tag="AC1LW8_001764"
     CDS             422..1144
                     /locus_tag="AC1LW8_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4773593.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDVGLNIEDLNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFN
                     ALDHVAQKNFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSF
                     NQTTLETWLLESQKEVIPYEEK"
     gene            1309..1506
                     /locus_tag="AC1LW8_001765"
     CDS             1309..1506
                     /locus_tag="AC1LW8_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4773594.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
     gene            1680..1751
                     /locus_tag="AC1LW8_001766"
                     /pseudo
     CDS             1680..1751
                     /locus_tag="AC1LW8_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2016..3609)
                     /locus_tag="AC1LW8_001767"
                     /pseudo
     CDS             complement(2016..3609)
                     /locus_tag="AC1LW8_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3566..5044
                     /locus_tag="AC1LW8_001768"
     CDS             3566..5044
                     /locus_tag="AC1LW8_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773595.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            5050..6213
                     /locus_tag="AC1LW8_001769"
     CDS             5050..6213
                     /locus_tag="AC1LW8_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773596.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTNLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            6230..8005
                     /locus_tag="AC1LW8_001770"
     CDS             6230..8005
                     /locus_tag="AC1LW8_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4773597.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            8043..10049
                     /gene="ggt"
                     /locus_tag="AC1LW8_001771"
     CDS             8043..10049
                     /gene="ggt"
                     /locus_tag="AC1LW8_001771"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIP4773598.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYYETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDNYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRVYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLLSPEIYDNDTH
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIA
                     WLARKKGYQVALKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(10362..11135)
                     /locus_tag="AC1LW8_001772"
     CDS             complement(10362..11135)
                     /locus_tag="AC1LW8_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4773599.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPRNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKLSEH"
BASE COUNT         3754 a         1676 c         2013 g         3693 t
ORIGIN      
        1 atttaggtaa aatgatagtt gctcaagatt taaatttaca agagattttc tttcataatt
       61 taatttattt cttctctgca atatttggtt ttctgacact tggtctgtta aatataggta
      121 tgttactcat aaattctggg atgttaggct tttttatagc acatggaatc tatagcaatc
      181 aattaaaatc tattctatta gtattagcgc cacacgctat atttgagatt ttagcacttt
      241 taattgcttc aacattctca ttaggttttt tatcatacct ttatcaaaga atggtaaaga
      301 aaaataaaat aaaaaataag atgttccata attttgtaac aacaataata actgttattg
      361 tattaacaat aatcgcatca ttattagaag tttatgtaac cataaattaa ggaagtgtat
      421 catgctagtt ttaaataatc ttcaaaaaaa atttactaat tcaaaaatgg ttttgaataa
      481 tgtgaatgct aagtttagaa gtgggagcat caattgcata gtgggagtaa atggggcagg
      541 taaaacgact ttgttaaata ttattagtag tatattgatg ccaacaaaag gtgatgtata
      601 tttaaatagt gaaagtattt ttgaaaatag cacactaaaa aaagagattt tttacacacc
      661 agtaaatccc tttttttatg aaaatttaag tgctaaagat aatttgtact taatatgtag
      721 cctatataat cggaaaatag atcaaataac tattgagaaa actattaaag atgtaggttt
      781 gaatatagaa gatctaaata tacctgtata taatttttca agtggtatga agcaaaaact
      841 aaattttgct tctatgttat tagtagattc aaatgtttta ttattagatg aaccatttaa
      901 tgcccttgac catgttgctc aaaaaaattt cacacgaatt ttaaagggat tagtttctaa
      961 agaaaagata ataattttca ctagtcattt gcctaataca attttagaac tctcagaaaa
     1021 tatttatttg ttaaaagatg gatgttttac agataaacga aaagcacagt catttaatca
     1081 aaccacgtta gaaacatggt tgttagaaag tcaaaaagag gtgatacctt atgaagaaaa
     1141 atgatttgtt agtaatgagt gtagtatata ttttaacttc agtcataatg ttctacatta
     1201 atggattaag taaacaattt tttacatatg tagttggttt cccaattatt tattttggtt
     1261 atatatgtct agttaatctt ataaaaaaag aagccggaat aattaaaagt gaaaaataat
     1321 ttgaaaatag ctagagtttc actatctatg actcaaaagg agctagcaaa taaagttgga
     1381 gtaactagac aaacaatatc tcttatagaa aagggtgtac ataacccctc tctatcacta
     1441 tgtaaaaata tttgttcggt gttgaataaa aatttggatg agatatttgg agagaaacca
     1501 caataaaaga gcttattgaa agataatcta aaagtcgaca atatatttct tctcagaaac
     1561 tgctattatt ttgaaaagaa aacacacgta ttataaattt aaaataccga aatatagcat
     1621 tattaaatca aaaatagaat taaaaaagaa aattaatcga gaaaagtatt aaatgtcatt
     1681 tatttgaatg tgttaattat gaaagaaaaa gagtaagtga cgttaaatat acattgacta
     1741 agctaagttg acacactcaa aaatctgaat attctaataa aatataaata atcccccgca
     1801 cttcacatag tggcggggga tttattctgg cgtcaaaatg cccaaagtat gggggtgact
     1861 cttaaatcat ggcaaagcac ctaaaacttg atatggtgcc gagccctaac ttagcacaat
     1921 atagttagta tgacaaagtc atctaacata acacatcaga gattgcctga tttattccag
     1981 accatacagt gatacagttg cacaacgcat gacttttatt tagcaataac tgctacttct
     2041 gaaataagtt gctttgcata gtctgattgc ggatgtttga taatatcttc tgtgttattc
     2101 agttcaacga tttcgccatt tttcataact gcaacgcgat cacatatttc attgataaca
     2161 cccatgtcat gtgtgatgaa taaataagtg atgccgaagt ctaactgtaa ttgttttaat
     2221 aactcgatga tatctttttg aattgaaacg tctaaagcgg acactgcctc gtcgcaaaca
     2281 atcactttag gttctacagc aagtgctctc gcgatactta cacgctgacg ttgcccacca
     2341 gataattcgt gtggatagcg atataagaaa ctttgatcta ggccaacctt ttctaacaac
     2401 gatacgacag ttttaataat gtcatcatta tctttgactt tcccatgaat gattagtggt
     2461 cgtttaatca catcaatgac tttaaatctt ggattaatag atgcgaatgg atcttgaaaa
     2521 atcatttgta tctcttgtcg taaagatttc aattcatcat ctttaaataa acttaatggt
     2581 aattcgttat accaaataaa gccttctgac acttccttta gaccgacgac cgtcttagct
     2641 aatgtcgatt tccctgaccc tgattcaccg acaatgccta atgtttcgcc ttttctaata
     2701 cccaagttaa tatcattaac tgctcggtat aggctgccac tcggtgatgt gtaatccacg
     2761 ctcacgcgat cgaattttaa taaaatatca ttgtttaacg gtcttggcgg acgcgtttga
     2821 tgaatatcag gaatcgcatc tattaagcgt tttgtatagg tatgttgtgg cgatttaaaa
     2881 aatactttca accgtgccac tttcaacgac acttccatct ttcattacaa tcacatcgtc
     2941 gcaaaattga tacacagcgc ctaaatcgtg agtgataaaa ataatagatg tttctgtgta
     3001 ctcataaagg gacttcatta actgcagtaa ttgattttgt gtactggcat ctaatgccgt
     3061 tgttggttca tctgcgatta aaatttgtgg ctttaaaatc aatgccattg ctatcatgac
     3121 acgttgacgc ataccaccag aaagttcatg tggataagca tcaaattgtc gagttgcatg
     3181 ttttatacct actttttcta aaatgtctat tgtcatcgac tttgcttcag atttagatac
     3241 acgtttatgt tgaaatagta cttctgtaat ttgtttgcca atcgttaatc ttggattcaa
     3301 cgaagagagt ggatcttgaa aaatcattga aatatcctta cctcgaattt gttgtaacgc
     3361 tgaagttgat aaattattta acgattgccc attaaaaata atttctcctg ttaatgtgtg
     3421 atctggataa tctggtagta gccctaaaat agatttagca gtaatacttt ttcctgatcc
     3481 tgattcacca acaataccta ggatatgttt ttttcgtaat tcgaaagaga cattttttac
     3541 cgcttgaact gtagtttcat cataattgaa ttgtacattc agactgttga cttctaataa
     3601 atttgacatg atgttgtgcc tccctattca caaatgtgta aatttattct atacttttgt
     3661 atatcatagt aatctactca agattatttt acaaggagtg attatcataa ttacttattt
     3721 agaatctttt aagtcgttgt atcacaaggc attttacaag tttgtattat cggtattaag
     3781 cttgccaata tttttatatg cagtgataaa gtttttcttt tctgctaaga gaaaaaattt
     3841 ttacgctaat aattctgaaa tttcagaaat tgaacgagcg ttacatcaaa aatataaata
     3901 tttatcgcag caaaagtcat ccacacaaat acataaagaa gcattaaaaa tattcaaggc
     3961 acaaagttct aatacgagtt caaagaatat tgaacaagca catttttcaa catactttga
     4021 aaatttatta tttcataagt tcatcatgat caaagtaata ttggccttgc cgatgttcat
     4081 cttattgact atttatttac agccattagt tagatatatt tttgagagaa ttgtcatggc
     4141 tgtgattgtc atcattggtg ttattgtcag tgtgtttacc attctgtatt tttcaccgct
     4201 tgatgcggct tatagcatac tgggacaaaa tgcaacaaag gcacagatac atcaattcaa
     4261 tgtattacat catcttaacg aaccttattt tattcaattg tgggatacca tcaagggtgt
     4321 ttttaccttt gacttaggta cgacttacaa agggaatgag gttgtgacta aagcagttgg
     4381 cgaaagaatt ccaattacaa taattgtcgc agtattagcg ctaattgtgg cattaattat
     4441 tgcaatacca attggtatta tcagtgcgat gaagcgaaat agttggcttg atatcacgtt
     4501 aatgataatt gcattaattg gtttatctat tccaagtttc tggcaagggc tattattcat
     4561 tttagcgttc tcattgaaat tggatatttt gccaccatct tatatgccag aacatccaat
     4621 atcgttgatt ttacctgtac ttgtcattgg aacaagtatt gctgcttcta tcacgcgtat
     4681 gacaaggtct tctgtacttg aagtaatgcg cagcgattat gttttaactg cttatgcaaa
     4741 aggattatcg acgacacaag ttgttattaa acatattttg aaaaatgcca ttattccaat
     4801 tgtaacgtta gttggtcttc tagtggcaga gttactaggc ggttcagcag tgacggaaca
     4861 agtatttaac attaatggta tcgggcgtta tatcgtccaa aaacaactaa tacctgatat
     4921 cccagcagtc atgggtgggg tcgtatatat atcaattgta atatctttag caaacttaat
     4981 tattgatata ttttatgctt taatcgatcc aaaattacgt agtgaaatta acgaaaggaa
     5041 gtgaggcata tggtaaaact tacaacaaag atagcttcct taaaactatt cgcaagttat
     5101 gctatagcaa cttatatttt agttatatta acgagtgcat taaatctttt taaaggttat
     5161 gtggccgata cgttctatat tgctgaaaca ttgctaatcg ttttaaccat cattttaatt
     5221 atcattttaa caacggaaca aacatggaag catcatgact tatggcgacg tatcgtcgaa
     5281 gtgttgttat tgttgatgac attaacaggc aacgtattta cattattaat gtttgtaagt
     5341 attagacgtt accaacgtac atcgcaaata catagttata acgggtggga atcgtttata
     5401 cgaaaaacta ctagacatcg tattgcgatt atcgggttac ttattttagt ctacatgctg
     5461 acattatcaa ttgtgtcaca atttacattt gatacgacat tggctactaa aaatcagttc
     5521 aatgcactgt tacatggacc gagtctagcc tatccgtttg gtactgatga tttcggtaga
     5581 gacttattta cacgcgtagt tgtaggaacg aatctgacat tttcaatttc aattatttca
     5641 gtagttattg cagttatttt tggtgtgtta ctaggcacta tcgcaggtta ttttaatcat
     5701 attgataatt taataatgcg aattttagat gtagtgtttg caattccatc attattgtta
     5761 gcggtggcaa ttattgcatc atttggagca agtattccaa atttaattat tgctttaagt
     5821 atcggtaata taccatcatt tgcacggaca atgcgtgcca gtgttttaga aattaaacgc
     5881 atggaatatg tagatgcagc acgtatcact ggtgaaaaca cttggaatat catatggcgt
     5941 tatattttac cgaatgcgat tgcgcctatg attgtacgtt tttcattaaa tataggtgtg
     6001 gttgtattaa caacaagtag tttaagtttc ctaggacttg gtgttgcacc tgatgtagct
     6061 gaatggggca acattttacg taccggtagt aactacttgg aaacgcacag taatttagct
     6121 attgtacctg gtgtttgtat tatgttcgtc gttttagcat ttaattttat aggtgatgca
     6181 gtgcgtgatg cactagatcc aagaattcat taaaaaggta gggatagatg tgaagaaaat
     6241 cattagtatc gcaattatag ttttagcgtt ggtattaagt ggttgtggtg tccctacgaa
     6301 atcagaagtg gctcaaaagt catcgaaagt tgaagtgaaa ggcgagcgac caacaataca
     6361 tttcctagga caagcaagtt atgaaaatga tatgaatatc gttaaagatc aattggaaaa
     6421 tgcaggattt aacgtgaaga tgaatatcca accagattat ggtagctatc gtacacaacg
     6481 tcaagccggc aattatgata tccaaattga tgactggatg acagtgtttg gtgacccgaa
     6541 ctatgctatg acggcattat ttagttctac aggatcaaat agtttattga aagataaaca
     6601 tgtagaccag ttgttaaata aagcttctac tcaaaatgaa gcagatgtta aacaaacata
     6661 taagcaaatt gaagatgaag ttgtatttga taaagggtat atggcgcctt tatatggatc
     6721 aaaaaagaat ttagtatatg acaataaagt gttagataaa aatagtgttg gattgccaaa
     6781 ttcacgtgca ttaatatggc aacaatttga ttacaacaat agtagagaac gagatacgcg
     6841 gccacttgtg atgacacaac aagatggtga aattcctaca ttggatccaa tacgttcaat
     6901 tgcgccgtca gtatattcaa ttaatatgaa tatgtacaca aggttattat tattagatga
     6961 aaatgatcat ttaacaacga aaggttcgtt aagtcatgat tatgctgtga ataaggacaa
     7021 taaagcattt tatttcttgt taagagatga tgattatttt gcgaaagtgg tcaatggaca
     7081 agcacgtaat actggagagc gtgtatcggc tgaagatgtt aagttttctt tagatagagc
     7141 acgtgataaa aagtctgtgc ctaacaataa tacttacaat atgcacaaac atataaatga
     7201 catcaagata ttaaaagatg aggacatcga tcagttgcgt aaagagaaag acaaggacga
     7261 taaatcaatc tatgataagt tgattaaagc ttataacgtc aaatcgttaa cgacagatgg
     7321 tcaaaaagta aataataaag acggtattta tcaaattgtt aaaattacga cagatcaatc
     7381 gatgcctcga gaggtaaatt acttaacaca ctcttcggca ggcattttat ctaaaaaatt
     7441 tgttaatcaa gtaaatcaag aatatccaaa aggatatggg gatagcagta caattcctgc
     7501 aaattcagat gggaaaaatg cgctgtatgc aagtggcgca tacattatga cacagaaaaa
     7561 tgcatatcaa gcaacgtttc aacgtaatcc aggattcaac gaaacagaaa aaggtagtta
     7621 tggaccagct aaaattaaaa atattacatt gaagtttaat ggtgacccga ataatgcatt
     7681 gtcagaactt agaaatcatt caattgatat gttggcagat gtgaatcaaa aacattttga
     7741 tttaattaag tcggataaaa atttaagcat tattcgcaaa aatggacgca agtcagtctt
     7801 tttaatgcta aatattaaaa aaggtatatt taagacgcat ccaaacttga gacaagcagt
     7861 agttaatgcg atagatcagg atcaatttat taagttttat cgtggcgata aatttaaaat
     7921 tgcatcaccg attacaccac ttgtcgatac tggtaacgag caacgtcaag atttagaaaa
     7981 agtagaaaaa gccatcaatc aataatgttt taaatattta acagaaagta ggaggatata
     8041 gtatggtcat taacttaaat gacaaacaga caaaaacatc taaagaaggg ttaatttccg
     8101 tatcacatcc tcttgcggct aaaattggta aggatgtatt agatcaaggt ggcaacgcca
     8161 tggatgcagt gattgcaatt caactggcgt tgaatgtggt agaaccattt gcatcaggta
     8221 ttggtggtgg cgggtatttg ctatattatg agcaaagtac tggcagtata actgcgtttg
     8281 atgcgcgtga gacagcccct gcacatgtag ataaacaatt ttatctagat gattcaggcg
     8341 aatataaatc attttttgat atgactacac atggtaaaac tgtcgctgtg ccagcaattc
     8401 caaagctgtt tgattatatt cacaagcgtt atgctaagtt gtcattggaa gatttaatta
     8461 atcctgcaat tgaactagct attgaaggtc atgcagccaa ttgggctact gaaaaatatt
     8521 cgcgccagca acacgcacga ttgacaaagt attatgaaac ggcacaagta tttacgcatg
     8581 aaaatcaata ttggcgtgaa ggtgattgga ttgtacaacc cgaattaggt aagacatttc
     8641 aaatattaag agaacaaggg tttaatgcat tttataaagg tgacattgcg aaacaattag
     8701 tcaatgttgt caaagcatgt ggtgggacaa tcactttaga ggatctagcc aaatatgaca
     8761 ttcagattaa agcgccaatc agtgcaacat ttaaagacta tgacatttat tcaatgggac
     8821 catctagttc tggcggtatc acggtaattc aaatattgaa gttattagaa catgtcgatt
     8881 taccatctat gggtccaaga tctgttgatt acttgcatca tttgatacaa gcgatgcatt
     8941 tagcatatag tgatcgtgcg caatatttgg cggatgataa ttttcatgag gtgcctgtac
     9001 agtcattaat tgatgacaat tatttaaaag cacgcagtac gctcattgat agcaataaag
     9061 caaatattga tatagagcat ggtgttgtgt ctgattgcat tagtcataca gatgttgaag
     9121 aaaatcatac cgaaacaact catttttgtg tgattgataa ggaaggtaat attgcttcat
     9181 ttacgacatc aatcggtatg atttacggat cgggtatcac gattccaggc tacggcgtgt
     9241 tattgaatac gacaatggat ggctttgatg tagtagatgg tggtattaac gaaattgcac
     9301 catataaacg accactaagt aacatggctc caacgattgt gatgtatcac gggaagccaa
     9361 tattaacagt aggtgcacct ggtgccataa gtatcattgc tagtgttgcg caaacattaa
     9421 tcaatgtatt agtgtttggc atggatattc agcaggctat agatgaacct agagtttata
     9481 gtagccatcc taatcgcatt gaatgggagc ctcaattttc acaatctaca atattagcat
     9541 tgattgcacg tggacatgca atggaacata aaccagatgc ctatattgga gatgtacatg
     9601 gactacatgt tgacttgaat acacgtgacg catcgggagg tgctgatgat acgagggaag
     9661 gtacagtgat gggtggtgag gtattatcaa ttagaaaaca accattactt agtcccgaaa
     9721 tatacgataa tgacacccat cgtgtatatt tcaatgatgt gcagttacct ttattggcag
     9781 atcaagtgcg atggatgcat gacaaatatt gggttgatga aagtgtcgtt agaatcatct
     9841 tctctgaagt tagtgcacat attgaagatt taagaagtta tgaaaacgca ggagaaaatt
     9901 atatagatat tgcctggtta gcacgaaaga aaggttatca agtggcattg aaagatgatg
     9961 gtttatactt aactgatgat acatatactt cagtgaaacg gaacacaaat gcatactata
    10021 gatatgatcg agatagtatc acaagatagt ttatacgctt gatatgaagt ttgtagtgga
    10081 taagggcttt tgttgaatga aaggattcga ttttgaactt tagcgtgacg cttgagtgta
    10141 gtaatgttga agttgttata agaaacgcta gtcgcctttg taaaatataa aacgccccac
    10201 aaattcgttt ttgatgtaca ccacaatagt tagtctaaaa tctaacttga tgtgattcgg
    10261 tacaattaaa tgaatgatgt gaggcgtttt tataatgtgt gattttagta tgttaaacga
    10321 taataaatga tttggttatt tctataatta catcataagt attaatgttc acttaattta
    10381 ttataagtaa ggctttcttt gtatataatt tggtcttttc caggacttga aaaataaaaa
    10441 taaagtttct gatcttgatg attattgcct tttaaatcac tcattccctt taattccacc
    10501 tttgttgatg catttctggg aatattatat ctacgtttta attgtttaac gtttggatta
    10561 tcattcgaca acttaccaga taaattataa taatcagtgg taggatcatg tgtgactttc
    10621 aaatcattgc tatttaaaat gcttgttaaa tcaccacttt gaatcaaaaa ttgattgttt
    10681 tcgatttttt gtttcagcgc gggatctttt acgtcttttg tgaaaacgat tttattatta
    10741 actactttta ctggataact tttgtatgtc gagtcagtag cattttttct atcgtttgta
    10801 gttgtgtcat attcaccagt tattttatgt gtgttcttat ctacctttaa caacatacgg
    10861 tcttctttta aaagctcatc tgatccaaca actgaataag aggattctat ataccatgtg
    10921 tcttgatcat tattttcata atggggatta tcgtgaccat caatttcata aagcgtttct
    10981 aagtttttaa taggatacgt acttagtact tttttaagac catctttcaa atgaatttgt
    11041 tcccacttca ttgccaaaaa cataccgcca ctgactacaa ttgaaataat aataattgct
    11101 gctaagttta accagaaaat tttatgtgct ttcata
//
LOCUS       DBNENW010000087        10874 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNENW010000087 DBNENW010000000
VERSION     DBNENW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.087"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(84..1943)
                     /locus_tag="AC1LW8_001773"
     CDS             complement(84..1943)
                     /locus_tag="AC1LW8_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HIP4773600.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            2154..5135
                     /locus_tag="AC1LW8_001774"
     CDS             2154..5135
                     /locus_tag="AC1LW8_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIP4773601.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDIIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(5247..6185)
                     /gene="manA"
                     /locus_tag="AC1LW8_001775"
     CDS             complement(5247..6185)
                     /gene="manA"
                     /locus_tag="AC1LW8_001775"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HIP4773602.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6199..8151)
                     /locus_tag="AC1LW8_001776"
     CDS             complement(6199..8151)
                     /locus_tag="AC1LW8_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HIP4773603.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSDLNKHSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(8228..10102)
                     /locus_tag="AC1LW8_001777"
     CDS             complement(8228..10102)
                     /locus_tag="AC1LW8_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP4773604.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHIPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRLVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(10342..10800)
                     /locus_tag="AC1LW8_001778"
     CDS             complement(10342..10800)
                     /locus_tag="AC1LW8_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773605.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKATHK
                     KTETEENDQQDDSADKADKNQNNGNNVTNDNQPSSQSNVPNNHSTAPSNTQPAQPKDS
                     NSNKNNTHDGGSQSSTNNQNNQQQNTPPANKSNNTNTSSKQTPQAQSPKN"
BASE COUNT         3495 a         1737 c         1952 g         3690 t
ORIGIN      
        1 taagaaatcg gacttatgaa cagaaatatg tttaagtccg aactccttgt ttatacttat
       61 aaattttacg ggtttaataa tacttattta cctgtaatat atgataattc ttcagcggca
      121 gctgcattga tagttctatg agaaatgata cctaatcctt taacattgga ttctgaaata
      181 acgatagaac catcactgtt aactttttca acaaatgcta catgaccgta atgttgatct
      241 gcaccaaatt gtccagcctc aaatacaaca gcagcatgac gttttggtgt atgacttact
      301 tgataatcac ggtattgagc tcgattattc caattatgtg catcacctaa atcacctgag
      361 atagatgtac caaattgttt catacggtta tatacgtacc aagtacattg accatgtgga
      421 tatggcatac tatcagatac ctcacggaaa ggtttgaatt catctgatga atcatcataa
      481 tccttgatag aacgttcata tttatctaaa tctggcatgc gttcatcgtc aaactgagtt
      541 aattgatagt gtttaataat actgtttaat ttcttagcat agtttggatc tgtagcatat
      601 gttttagata agtgtgatgt tgcatcttta taagaatcgg cttccgattt ccatgttggt
      661 ttataaattg ttcgattgcc atcaatacca tttttaataa ggtcagagta atcttttagt
      721 gattctttcg tgcttggata ttttcggaat ccagcattaa tactatacaa tttattacca
      781 tcagcttcta atgtgttaaa aggaacagaa ttcccttcaa aagcaccttt gataccgaat
      841 aaattatggt ttggtgactt agctaaagca ctacgacctg agtcagattc taagattgct
      901 tgggcaatca tgacagacgc ataaatatcg ttatcttgac caatgcgatg tgcatcttta
      961 gcaattgatt tgacaaattg acgtgtatct tttgagtcaa caacgttaaa ttgtccgcta
     1021 tcatcattgt tagatatact aggatctgtt tcgaataatg atgttgcacg tgtatccttt
     1081 tgattaacat cgttattgaa tgattgagca ggtttagatt tatgtttcaa ttcatcttgt
     1141 gttggtaact gtggattctt tgtattagat ttttcatttt tgtctttttt agattgagat
     1201 gcataatctt tttgtgtttt ctttgcatct tcactgtatt gatccaaaat agagtctaaa
     1261 gccgaatctg acattgattg attatctttc gatgaagatt tttgatttgc tttatcgtca
     1321 cttgctggtt gactatttga ttgattaggt tgtgttggct ttggcgaatt tggttgctta
     1381 ttagatgtac ttggttttgt attgtttgat ttaggtgctt tttgattgtc tgctttatct
     1441 tgtttagatg attgcgtatc agtgtcattt ttgatgctat tgtcactgtt tttattcgaa
     1501 tcatttgttg acttttcgcc attacgaggt tgttcgtaat cagaaatatc cgaatttaaa
     1561 ttgaataggt tttggattaa agttgttaat gagtaattat catcgtattt atttttggtt
     1621 agcaattggt ttatattggt ttgtggtaaa ttcttataaa taaaatcaat gatattgtta
     1681 gagtctgaag tgctgtcgtc tatagtttta aattttttgt cgttattgtc ttggttactt
     1741 gtattattgt tgtctgcttt atcaatatct ttacttgtag tatccttaga agtttcatcg
     1801 tcattagatt tttttgaatc atgagatgtt gtcttagctg tagtatcttt ttgaggtgta
     1861 tcagcataag cggtaggtga aactaaagta ggtaatacga gcgtagttga tagcaaataa
     1921 attaaaattt tatttttagg catatttcgt attctccctt gaaaaatata ataattaagt
     1981 gtgataataa actatgattt gttataattt atcgtatgct gaaaatagtt gataggtatc
     2041 aatcgactaa atatcttcca gtaaattgat tatactaatt cacaacgcaa aaataaatta
     2101 atttacaaaa aatatataaa aaatatgaat aattcctaca taggagtgtg acaatgaaga
     2161 acgcatttaa attatttaaa atggatctga agaaagtagc taagacgcca gctgtgtgga
     2221 ttatcttagc aggcttagct attttgccat cgttctacgc ttggtttaac ttatgggcaa
     2281 tgtgggatcc atatggcaac acgggacaca tcaaggtcgc agtcgttaat gaagataaag
     2341 gcgacataat cagagggaaa aaagttaatg tcggtaatac gatggttaat acactcaaga
     2401 aaaataaaag ttttgattgg cagtttgtaa gtagagagaa agctgatcat gagataaaaa
     2461 tgggtaaata ttttgcaggt atttacatcc catctaagtt tacacatgaa attaccggga
     2521 cactacgtaa gcagcctcaa aaagcagatg tagaatttaa ggtgaatcag aagattaacg
     2581 ctgttgcgtc taagctaaca gatactggtt cgtcagttgt cgttgaaaaa gcgaatgaac
     2641 aatttaataa aacagtaact cgagcattat tagaagaagc taacaaagca ggtttaacta
     2701 ttgaagaaaa tgtgccgaca attaataaga taaaaaatgc ggtatattcg gcagataaag
     2761 ctttacctaa gattaatgac tttgcgaata aaattgtata tttgaataac caccaagcgg
     2821 atttagataa atatgccaat gattttagaa aactaggaaa ttataaaggt gatattttag
     2881 atgctcagaa aaaattaaac gaagtcaatg gtgctattcc gcaacttaat gaaaaggcta
     2941 agttgatatt agctttaaat aattatatgc cgaaaattga aaaagcgtta aattttgcag
     3001 ctgatgacgt gccagcgcag ttccctaaaa ttaatcaagg acttaacatt gcgagtcaag
     3061 ggattgatca agctaatgga cagttaaatg atgccaaagg cttcgtcaca caagttagaa
     3121 gtagagtcgg tgattatcaa gatgcaattc gacgcgcgca agatttaaat cgaagaaacc
     3181 agcaacagat tcctcaaaat agcgcggcga acaacgaaac atcaaatagt gcacctgcag
     3241 ctggtaatgg tgtagcatca acgccaccaa gtgcaccaag tggcgatact gcaccaaata
     3301 ataatgttac gcaaaatacc gcaccaaata gtaataatgc gcctgtatcg actacaccac
     3361 aaagtacaag cgggaaaaaa gatggtcaaa gttttgcaga tataacaaca acacaagtca
     3421 gcacagctaa cgagaacaca caaaacatta cagataaaga tgttaaatca atggaagcgg
     3481 cattaacggg ctctttatta tcattatcaa ataatttaga tacccaagcg aaagccgcac
     3541 aaaaagatag tcaggcatta cgtaatattt cgtatggcat tttagcatcg gacaagcctt
     3601 ctgattttag agagtcttta gataatgtta agtccggttt agaatacaca acgcaatata
     3661 atcaacaatt tatcgataca ttaaaagaga ttgagaagaa tgaaaatgtt gatttatcaa
     3721 aagaaattga taaggtaaaa gcagctaata atcgaattaa tgaatcatta aggttagtta
     3781 atcaattaag caatgcatta aagaatggta gttcaggaac tgctgaagct actaaattac
     3841 tagatcaact ttcaaaacta gattcatcat tatcatcatt tagagattat gttaaaaaag
     3901 atcttaacag ctctttagta tcaatatcac aacgtattat ggatgaattg aacaaagggc
     3961 aaactgcatt atccaatgtt cagtctaaat taaatacaat tgatcaagtc attaacagtg
     4021 gacaagctat tttaaaaaat ggtaaaacac gtattgatcg cttacaaaca gtattaccaa
     4081 gtattgaaca acaatacatt agtgctgtta aaaatgctca agcaaacttc ccgaaagtga
     4141 aaagtgatgt agctaaagct gctaactttg tgcgcaatga cttaccacag ttggaacagc
     4201 gattaactaa tgcgacagca agtgtgaata aaaatttacc aacgttatta aatggttatg
     4261 atcaagcggt aggattacta aataaaaatc agccacaagc gaaaaaggct ttatcagatt
     4321 tagctgattt ttctcaaaat aaattgcctg atgttgaaaa agatttgaaa aaagcgaata
     4381 aaattttcaa gaaattagac aaagatgatg cagtcgacaa attaatcgac acacttaaga
     4441 atgatttgaa aaagcaagcg ggtattattg caaatcctat taataagaag actgttgatg
     4501 ttttcccagt taaggattat ggttcaggta tgacaccatt ctatactgca ctgtcagtat
     4561 gggtaggtgc actcttgatg gtaagtttat taacggttga taataaacat aagagtctag
     4621 agtcagtctt aacgacaaga caagtgttct taggtaaggc aggattcttt ataatgcttg
     4681 gtatgttgca agcactcatt gtatcggttg gagatttgtt aatcctaaaa gcaggagttg
     4741 agtcacctgt attatttgta cttataacga ttttctgttc gattattttc aactcaatcg
     4801 tatatacgtg cgtatcatta cttggtaatc caggtaaagc cattgcaatc gtattgcttg
     4861 tattacaaat tgcaggtggt ggaggaacat tcccaattca aactacgcca caatttttcc
     4921 aaaacatttc gccatactta ccatttacgt atgcaattga ttcattacgt gaaacagtag
     4981 gcggtattgt tccggaaatc ctaattacaa aattaattat attaacgtta tttggcatag
     5041 gattcttcgt tgtaggttta attttaaaac ctgtaacaga tccattgatg aagcgcgtat
     5101 ctgaaaaagt tgaccaaagt aacgttacag aataaaaatt aaatccacac attagggtta
     5161 tagctcctta atgtgtggat ttttatgttt ttagacagaa gagatagtaa tttctgtctt
     5221 ttatgggacg ggtgttatca ttgctattat ccaggatgac ttactatagc actaatatta
     5281 ccgacaaagt gaatatcctc gtcttccgta gttaaaataa agctagaacc tttttggatg
     5341 tcatagtgct tatcgtttac tgttaaagta ccagtaccat cgataatagt aactaagcaa
     5401 taagcatgtg gtttattgaa ttttaaatct ccatgaatat cccatttata tactgcaaaa
     5461 tattgattat ctacaaattg agttacagtg tgtgtgtcga tgtgagttgt tataggagta
     5521 gtatttggtt catgattgcc taattcaatc acatctttac tttgctctaa gtgcaaatca
     5581 cgcaattgac cattttgatc tcgtctatca tagtcataaa tacggtatgt cgtatcggat
     5641 gattgttgtg tctctaaaat taaaataccc gaaccaatgg catggacagt gccagcaggc
     5701 acataataaa agtcaccggg cttaacaggt atacgtttga aaagactgtc aaattcatga
     5761 ttatcaatca tgtctattaa cgtctgttta ttatgtgcat gtacgccata tataatttca
     5821 gcacctgggg ctgcatctaa aatataccaa cattctgttt tacctagttc gccttcgtgt
     5881 tttaaagcgt agtcatcatc tggatgaact tgaacagata atttatcatt ggcatctaat
     5941 actttagtta gcagagggaa actatctcgt gaatcattat cgaataattc acgatgttgt
     6001 gaccaaagtt gatctagggt catatccttg tatggaccat tgataattgt attaggacca
     6061 tttggatgtg cagaaattgc ccagcattca ccagttgtct cattagggat atcatagtta
     6121 aatgctttta atgcatgacc gccccaaatt ctgtctttaa aaacgggttg taaaaataat
     6181 gccatagtta aaactcctct atattttcat taataagtta taaatttctg tagtactgtt
     6241 tgcattaatt agtgattggc gtgtctcatc attcattaac gctttagata agcgctgaag
     6301 tatttttaaa tgtgtatcct gactgttgtt tggtacggca attaagaata tcaattgagg
     6361 tagactacca tctagactgt cccatttaac accatgatta tttttcataa cagctacaat
     6421 cggttgtttt acaacatcag actttgcatg tggaatggcc acgttcatgc caatagctgt
     6481 cgtagactcc atttcacgtt ctaggattgc attttttaaa tgcgatgtgt gctctacata
     6541 acggcaaatt ttaagtttat gaatcaacat atcaattgct tcgtttcgag acatgtcgtg
     6601 atcagtaatt atcatagttt gttgatcaaa aacatgagaa tgtttattga gatctgaatg
     6661 tttcgcggtt ttatctacat tgtcaacctc tgtatcatgt tgtgtaatat ctgtatcatg
     6721 aagttgcgtg tgttgcgctg gtgcatctac tgctataact ggtgtattgc gttttaataa
     6781 tagtacagta gtcattgtga caagactacc tactatcact gcaaagataa accataatac
     6841 atgatcaata ccacctaata cagccacgat tggacctcca tgtgcgactc tatcgccgac
     6901 accaccaatg gctgcaatga ctgatgcaat cattgcacca atgatgtttg caggtataat
     6961 gcgcaatgga tcttgggctg cgaaaggaat agcaccttca gtaataccaa acagtcccat
     7021 agtgaaggaa gccttaccca tttctctttc ggaatgattg aatttatact tttgaacaaa
     7081 cgttgctaaa cctaaaccga ttggtggtgt acatacagca actgcgacca tacccataac
     7141 ggcgtaatta ccttcagcaa taagtgctga gccaaataaa aatgctactt tgtttactgg
     7201 accgcccata tcgaaggcaa tcatcgcacc tataatcatc gcaagtataa taatattagc
     7261 accttgcata ctttttaacc aggttgttaa tgcctcaaaa atattagaaa ttggtgcacc
     7321 gattaaaaat ataaatatca atcctacaac gaccgatgaa ataatgggaa taataatgat
     7381 aggcataatt ggtgccattg cttttggaac tttaatatct ttaatccact ttgcgatata
     7441 acctgctaag aaaccagcaa caataccacc taaaaatcct gcgcctgcat cactgccata
     7501 aaaactaccg tcagcagcga tagcgccgcc aatcatacca ggaacaagac cgggcttgtc
     7561 agcgatacta acagcgatat atccagctag tattggaacc ataaatttaa aggctaaact
     7621 accaatgttt tcaatggatt tccaaaatga atcatctggg atgactaatc cttttgatgt
     7681 cgtttcaccg cctagagtca gcgcgatggc gataaggagt ccaccaacta cgataaaagg
     7741 aaccataaac gatacaccgt tcattaaatg ttgatacacc atttgaatac cacttttaga
     7801 cttaccgcga tctttcgaat gataatttgt ttcagattga taaataggcg catcttgatt
     7861 aatgatacgt tgaattagac ctctcggatt atgaatccct tcgcgaacat tttcattaat
     7921 caaccgttta ccaacaaatc gggacagatc aacttgttta tcagctgcaa ttatgacacc
     7981 gtcagcttct tcgatgtctt gcgtagttaa aacattttca gcaccaacac cgccctgtgt
     8041 ctctacttta atatccacac ccatttcttt tgctacctgc tcaagctttt cttgagccat
     8101 atatgtatgt gcaatgccat ttgggcatga ggtaatagct acaattttca taaaatcatc
     8161 tccttttcta tattgtaagc gtattctcga tactaaaaaa aagaataatt accgttacta
     8221 gtggcaatta ttcttgtaag tattcaaata actgttgctt taaactatga tcatctaaac
     8281 tacataaatg gttcactgaa tcatcatcca agttagcaat taattgcatc atttgttttg
     8341 taaaagcttt gtctttatgc gaaatcgcta agaaaaagac aagtttgaca tcgtgttgtc
     8401 gccaaggaaa aacatctttt gtgcgaaaaa taagcacatg tgattgtaaa actttttcag
     8461 gatctccatg aggaatcgcc ataaaattac ctatgtatgt agaagatgat ttctcacgct
     8521 ctaaagctga ttcgatatat ccttctacaa tcgcatgatg tgcttgtaat attttttgag
     8581 cttcttcaaa aatttgcaca gtatgccgtg atttttgttc agtatttacg acaaggaaat
     8641 tgacagtgtc catatgatga tgtgcttgaa ctggattttg cttttgcttc acaacgagtc
     8701 tgattttgtg acggtcatct tcagaaaata atggtgcaac cttgatagtc gttaggtgct
     8761 taggaagtat gtttagcgtt tgttttggaa tatcatgggt cgttattaat aaatctacat
     8821 tgtcaaagtg atagtgtgtt atattttcta gtttaatcgt atttatcact gacaactctt
     8881 cggataagtt atttatttta gtttctaaaa aattcgacac acctagacca taataacaag
     8941 caatgactac atttaattgt gttttggtac gacgctcgat ggcagcttga aaatgaattg
     9001 ttaaaaatgc aatttcatct tcgctcatct ctatatcagt atcaattgct aatttatcaa
     9061 tcgcttcaaa aagtgtgtta aacacaaagg gatagagttt tttaatctct ataactaaag
     9121 gattgtttaa ataaatgttt tgagtgatac gtaaatatgc tttactaaaa tgattatata
     9181 aattttgttg taaaatcgaa tcttcattga aaggtatatg aatacgttgc tgcatcaatt
     9241 cgattaagcg atcaatataa ctttgtataa atatacgttc tatgccaata tcgagtttat
     9301 taaaatgata agcaataaag aatgaaaaca tattgattac tttttcgttc aagtcataac
     9361 ctaatctttc gttgatttgc ttaatgcaag attgagatat caattttgaa tcgtggttta
     9421 acgtgtctag ctcacttaaa gattgcttac gtttaatgat taaaatgaga tgaattaaaa
     9481 gctgttgtat ttgaatatca gttgtttcaa tactatgttg ttgaagtgtc tcttgtataa
     9541 tatgcgaaat catcctttgg tgtgaatcag gtaattcatt taaaattagg tcttcaacat
     9601 gtacatgccc tgatgataat tgatttaaat ggatgatggc attagtgata tcattatctg
     9661 ttccatcgac cacaatacct tgttttttct tgattgttaa agcgatatta aatttttgac
     9721 accattgttg aattcgaacg aaatagtcgt tgagcacagt tttagataat agataatctt
     9781 gctctagttg ttgcaatgtc tgcgatttat tgtgcattag cagttgatac gccatagata
     9841 ataatagttt ttctttatat gattgaatat gcgtgtaacg ttcagttatc gtttctaagg
     9901 tatactgcga tgtatttaat tgatatcctt tagatttaat actaacgatg atatcatcca
     9961 taaaattgct attgataaca tgaatatcat tgcgaactgt gcggtttgaa acattgacat
    10021 gttcagcaat ttcattagaa ctaatgtgct ttgatggatt tttaataaaa aactggagta
    10081 gttttaagtg tctatcaagc atttaacatg tacctccttt ctaaattttc cgtgtaagcg
    10141 tttttaaagg tgtattaata ttattaaaca tgagagctta tacatacgtc aatgacatta
    10201 aagcgaactt ttatatgaat ttaacagagt gcgaattatg caaataaaga acagcagtaa
    10261 gatatttcaa atagaaaaat atcatattgc tgttcttctg aatttatgta cctgatactt
    10321 gtggacgtat caggtaaatg attaattttt aggtgattgt gcttgaggtg tttgtttaga
    10381 agacgtattg gtattatttg atttattagc aggtggtgtg ttctgttgtt gattattttg
    10441 gttgtttgtt gacgattgac taccaccatc gtgagtatta tttttatttg aatttgagtc
    10501 ttttggctga gctggttgcg tattcgatgg tgctgttgaa tggttgttag gaacatttga
    10561 ctgtgaagat ggttgattgt cattagtaac gttattgcca ttattttgat ttttatctgc
    10621 tttgtctgca ctatcatctt gttgatcatt ttcttcggtt tctgtctttt tatgcgtagc
    10681 tttattttct tgttcttggt tattacttga tgatgtagaa tgatttattg cagcataaat
    10741 tgcgaaccca agtgctccga ttaatacgat aattacgatg gtaataagaa gttttttcaa
    10801 atataacgcc tccatgttaa tgaaaagtag ttgatacgtg aagctaaata atttatttca
    10861 atattgccat aatc
//
LOCUS       DBNENW010000088        10835 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNENW010000088 DBNENW010000000
VERSION     DBNENW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.088"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            55..300
                     /gene="yaaA"
                     /locus_tag="AC1LW8_001779"
     CDS             55..300
                     /gene="yaaA"
                     /locus_tag="AC1LW8_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIP4773606.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            297..1409
                     /gene="recF"
                     /locus_tag="AC1LW8_001780"
     CDS             297..1409
                     /gene="recF"
                     /locus_tag="AC1LW8_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIP4773607.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            1419..3353
                     /gene="gyrB"
                     /locus_tag="AC1LW8_001781"
     CDS             1419..3353
                     /gene="gyrB"
                     /locus_tag="AC1LW8_001781"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIP4773608.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            3390..6053
                     /gene="gyrA"
                     /locus_tag="AC1LW8_001782"
     CDS             3390..6053
                     /gene="gyrA"
                     /locus_tag="AC1LW8_001782"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIP4773609.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(6140..6952)
                     /locus_tag="AC1LW8_001783"
     CDS             complement(6140..6952)
                     /locus_tag="AC1LW8_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIP4773610.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            7278..8792
                     /gene="hutH"
                     /locus_tag="AC1LW8_001784"
     CDS             7278..8792
                     /gene="hutH"
                     /locus_tag="AC1LW8_001784"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIP4773611.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIESEDLGYQS
                     EWIAALAHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    8872..9081
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9171..10457
                     /gene="serS"
                     /locus_tag="AC1LW8_001785"
     CDS             9171..10457
                     /gene="serS"
                     /locus_tag="AC1LW8_001785"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIP4773612.1"
                     /translation="MLDIRLFRNEPVTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
BASE COUNT         3784 a         1625 c         2219 g         3207 t
ORIGIN      
        1 tgaaatttgt gacaaaaaaa ggtataatat attaatgaca tacaaagaaa tggagtgatt
       61 attttggttc aagaagttgt agtagaagga gacattaatt taggtcaatt tctaaaaaca
      121 gaagggatta ttgaatctgg tggtcaagca aaatggttct tgcaagacgt tgaagtatta
      181 attaatggag tgcgtgaaac acgtcgcggt aaaaagttag aacatcaaga tcgtatagat
      241 atcccagaat tacctgaaga tgctggttct ttcttaatca ttcatcaagg tgaacaatga
      301 agttaaatac actccaatta gaaaattatc gtaactatga tgaggttacg ttgaaatgtc
      361 atcctgacgt gaatatcctc attggagaaa atgcacaagg aaagacaaat ttacttgaat
      421 caatttatac cttagcttta gcaaaaagtc atagaacgag taatgataag gaactcatac
      481 gttttaatgc tgattatgct aaaatagaag gtgagcttag ttatagacac ggcacgatgc
      541 cattaacaat gtttataact aaaaaaggta aacaagtcaa agtgaatcac ttagagcaaa
      601 gtcgtctaac tcaatatatt ggacacctca atgtggttct atttgcgcca gaagatttga
      661 atattgtaaa aggctctcct caaataagac gacgctttat agatatggag ttgggccaaa
      721 tttctgctgt ttacttaaat gatttagctc aataccaacg tattttaaag caaaagaata
      781 attacttaaa gcagttacaa ttaggccaaa aaaaggactt aacaatgttg gaagtattaa
      841 atcagcagtt tgctgaatat gcaatgaaag taactgataa acgtgcacat tttattcaag
      901 agctagagtc gttagctaaa ccgattcatg ctggtatcac aaatgataaa gaagcgttgt
      961 cgctgaatta tttacctagt cttaaatttg attatgctca aaatgaagcg gcacgacttg
     1021 aagaaattat gtctattctt agcgataata tgcaaagaga aaaagaacga ggcattagct
     1081 tattcggacc acatcgagat gatataagtt ttgatgtgaa tggcatggat gctcaaacat
     1141 atggttctca aggacagcaa cgtacaacgg ctttgtccat taaattagct gaaattgagt
     1201 taatgaatat cgaagttggg gaatatccca tcttattatt agacgatgta ctcagtgaat
     1261 tagatgattc gcgtcaaacg catttattaa gtacgattca gcataaagta caaacatttg
     1321 tcactacgac atctgtagat ggtattgatc atgaaatcat gaataacgct aaattgtatc
     1381 gtattaatca aggtgaaatt ataaagtaac agaaagcgat ggtgactgca ttgtcagatg
     1441 taaacaacac ggataattat ggtgctgggc aaatacaagt attagaaggt ttagaagcag
     1501 tacgtaaaag accaggtatg tatataggat cgacttcaga gagaggtttg caccatttag
     1561 tgtgggaaat tgtcgataat agtatcgatg aagcattagc tggttatgca aatcaaattg
     1621 aagttgttat tgaaaaagat aactggatta aagtaacgga taacggacgt ggtatcccag
     1681 ttgatattca agaaaaaatg ggacgtccag ctgtcgaagt tattttaact gttttacatg
     1741 ctggtggtaa atttggcggt ggcggataca aagtatctgg tggtttacat ggtgttggtt
     1801 catcagttgt aaacgcattg tcacaagact tagaagtata tgtacacaga aatgagacta
     1861 tatatcatca agcatataaa aaaggtgtac ctcaatttga cttaaaagaa gttggcacaa
     1921 ctgataagac aggtactgtc attcgtttta aagcagatgg agaaatcttc acagagacaa
     1981 ctgtatacaa ctatgaaaca ttacagcagc gtattagaga gcttgctttc ttaaacaaag
     2041 gaattcaaat cacattaaga gatgaacgtg atgaagaaaa cgttagagaa gactcctatc
     2101 actatgaggg cggtattaaa tcgtacgttg agttattgaa cgaaaataaa gaacctattc
     2161 atgatgagcc aatttatatt catcaatcta aagatgatat tgaagtagaa attgcgattc
     2221 aatataactc aggatatgcc acaaatcttt taacttacgc aaataacatt catacgtatg
     2281 aaggtggtac gcatgaagac ggattcaaac gtgcattaac gcgtgtctta aatagttatg
     2341 gtttaagtag caagattatg aaagaagaaa aagatagact ttctggtgaa gatacacgtg
     2401 aaggtatgac agcaattata tctatcaaac atggtgatcc tcaattcgaa ggtcaaacga
     2461 agacaaaatt aggtaattct gaagtgcgtc aagttgtaga taaattattc tcagagcact
     2521 ttgaacgatt tttatatgaa aatccacaag tcgcacgtac agtggttgaa aaaggtatta
     2581 tggcggcacg tgcacgtgtt gctgcgaaaa aagcgcgtga agtaacacgt cgtaaatcag
     2641 cgttagatgt agcaagcctt ccaggtaaat tagccgattg ctctagtaaa agtcctgaag
     2701 aatgtgagat tttcttagtc gaaggggact ctgccggggg gtctacaaaa tctggtcgtg
     2761 actctagaac gcaggcgatt ttaccattac gaggtaagat attaaatgtt gaaaaagcac
     2821 gattagatag aattttgaat aacaatgaaa ttcgtcaaat gatcacagca tttggtacag
     2881 gaatcggtgg cgactttgat ctagcgaaag caagatatca caaaatcgtc attatgactg
     2941 atgccgatgt ggatggagcg catattagaa cattgttatt aacattcttc tatcgattta
     3001 tgagaccgtt aattgaagca ggctatgtgt atattgcaca gccaccgttg tataaactga
     3061 cacaaggtaa acaaaagtat tatgtataca atgataggga acttgataaa cttaaatctg
     3121 aattgaatcc aacaccaaaa tggtctattg cacgatacaa aggtcttgga gaaatgaatg
     3181 cagatcaatt atgggaaaca acaatgaacc ctgagcaccg cgctctttta caagtaaaac
     3241 ttgaagatgc gattgaagcg gaccaaacat ttgaaatgtt aatgggtgat gttgtagaaa
     3301 accgtagaca atttatagaa gataatgcag tttatgcaaa cttagacttc taagcgctgt
     3361 gaactgaact tttgaaggag gaactcttga tggctgaatt acctcaatca agaataaatg
     3421 aacgaaatat taccagtgaa atgcgtgaat catttttaga ttatgcgatg agtgttatcg
     3481 ttgctcgtgc attgccagat gttcgtgacg gtttaaaacc agtacatcgt cgtatactat
     3541 atggattaaa tgaacaaggt atgacaccgg ataaatcata taaaaaatca gcacgtatcg
     3601 ttggtgacgt aatgggtaaa tatcaccctc atggtgactt atctatttat gaagcaatgg
     3661 tacgtatggc tcaagatttc agttatcgtt atccgcttgt tgatggccaa ggtaactttg
     3721 gttcaatgga tggagatggc gcagcagcaa tgcgttatac tgaagcgcgt atgactaaaa
     3781 tcacacttga actgttacgt gatattaata aagatacaat agattttatc gataactatg
     3841 atggtaatga aagagagccg tcagtcttac ctgctcgatt ccctaacttg ttagccaatg
     3901 gtgcatcagg tatcgcggta ggtatggcaa cgaatattcc accacataac ttaacagaat
     3961 taatcaatgg tgtacttagc ttaagtaaga accctgatat ttcaattgct gagttaatgg
     4021 aggatattga aggtcctgat ttccctactg ctggacttat tttaggtaag agtggtatta
     4081 gacgtgcata tgaaacaggt cgtggttcaa ttcaaatgcg ttctcgtgca gttattgaag
     4141 aacgtggagg cggacgtcaa cgtattgttg tcactgaaat tcctttccaa gtgaataagg
     4201 ctcgtatgat tgaaaaaatt gcagaactcg ttcgtgacaa gaaaattgac ggtatcactg
     4261 atttacgtga tgaaacaagt ttacgtactg gtgtgcgtgt cgttattgat gtgcgtaagg
     4321 atgcaaatgc tagtgtcatt ttaaataact tatacaaaca aacgccactt caaacatcat
     4381 ttggtgtgaa tatgattgca cttgtaaatg gtagaccgaa gcttattaat ttaaaagaag
     4441 cgttggtaca ttatttagag catcaaaaga cagttgttag aagacgtacg caatacaact
     4501 tacgtaaagc taaagatcgt gcccacattt tagaaggatt acgtatcgca cttgaccata
     4561 tcgatgaaat tatttcaacg attcgtgagt cagatacaga taaagttgca atggaaagct
     4621 tgcaacaacg cttcaaactt tctgaaaaac aagctcaagc tattttagac atgcgtttaa
     4681 gacgtctaac aggtttagag agagacaaaa ttgaagctga atataatgag ttattaaatt
     4741 atattagtga attagaagca atcttagctg atgaagaagt gttattacag ttagttagag
     4801 atgaattgac tgaaattaga gatcgtttcg gtgatgatcg tcgtacagaa attcaattag
     4861 gtggatttga agacttagag gacgaagact taattccaga agaacaaata gtaattacac
     4921 taagccataa taactacatt aaacgtttgc cggtatctac atatcgtgct caaaaccgtg
     4981 gtggtcgtgg tgttcaaggt atgaatacat tggaagaaga ttttgtcagt caattggtaa
     5041 ctttaagtac acatgaccat gtattgttct ttactaacaa aggtcgtgta tacaaactta
     5101 aaggttatga agtgcctgag ttatcaagac agtctaaagg tattcctgta gtgaatgcta
     5161 ttgaacttga aaatgatgaa gtcattagta caatgattgc tgttaaagac cttgaaagtg
     5221 aagacaactt cttagtgttt gcaactaaac gtggtgtcgt taaacgttca gcattaagta
     5281 acttctcaag aataaataga aatggtaaga ttgcgatttc gttcagagaa gatgatgagt
     5341 taattgcagt tcgcttaaca agtggtcaag aagatatctt gattggtaca tcacatgcat
     5401 cattaattcg attccctgaa tcaacattac gtcctttagg ccgtacagca acgggtgtga
     5461 aaggtattac acttcgtgaa ggtgacgaag ttgtagggct tgatgtagct catgcaaaca
     5521 gtgttgatga agtattagta gttactgaaa atggttatgg taaacgtacg ccagttaatg
     5581 actatcgttt atcaaatcgt ggtggtaaag gtattaaaac agctacgatt actgagcgta
     5641 atggtaatgt tgtatgtatc actacagtaa ctggtgaaga agatttaatg attgttacta
     5701 atgcaggtgt cattattcga ctagatgttg cagatatttc tcaaaatggt cgtgcagcac
     5761 aaggtgttcg cttaattcgc ttaggtgatg atcaatttgt ttcaacggtt gctaaagtaa
     5821 aagaagatgc agaagatgaa acgaatgaag atgagcaatc tacttcaact gtatctgaag
     5881 atggtactga acaacaacgt gaagcggttg taaatgatga gacaccagga aatgcaattc
     5941 atactgaagt gattgattca gaagaaaatg atgaagatgg acgtattgaa gtaagacaag
     6001 atttcatgga tcgtgttgaa gaagatatac aacaatcatc agatgaagaa taataaaaaa
     6061 taagacttcc ctatatgtag gggagtctta tttttatgct agaaagtaat gctttactat
     6121 attcaatgat tagtaatgac taactttcta attgtttcat tgcgtaaggt atttcattga
     6181 taagtcttga tggtggcacc acatacatat cttttgcaag gttttcgcca ataaaactat
     6241 gtgtatatgt ggcactcata accgcttctt ttaagttatc aaattgaccg acaaagcttg
     6301 taatcatacc agcaagtgta tcgcccatac caccagtcgc cattgctggg ctaccgattg
     6361 tcaatttaaa gtcttcatct ttaaagaaaa tttcagtacc atgtttttta agtacaacag
     6421 ttgcacctaa acgatcaact gcttcacgat tacgctcata tgtctgttcc tcaataggaa
     6481 taccacttaa tcgttcccat tctttgaggt gtggtgtaaa gatcacacga catgtaggta
     6541 attgcggttt cagtttacta aagattgtaa tcgcatcgcc gtctacgatt aaattttgat
     6601 gcggttgtat attttgtagt aggaatgtaa tggcattatt tcctttgaaa tcaacgccaa
     6661 gacctggacc aattagtata ctgtcagtca tttcaatcat tttcgtcaac attttcgtat
     6721 cattaatatc aataaccatc gcttctgggc aacgagaatg taatgctgaa tgatttgttg
     6781 gatgtgtagc tacagtgatt aaaccactac cgctaaatac acatgcacga gccgctaaca
     6841 taatggcacc acctaagtta gcagatccac caattaataa aattttgcca taatcacctt
     6901 tatgtgaatc ttctttacgc ttaggaatgt taatagaatt taacgtttcc atagtgatat
     6961 aacctcccat gtaaaagcct tttccgaatt tattcaattt taaaaatata tagtaacttt
     7021 taacaaaatg tattataaat ttctgaattc attattattt gtcgttaaat acaatagaaa
     7081 atactatacc tgtatatgca attcgtcaat agataaatta ttaaatatgc ttacaacaat
     7141 cttaatatcc tttaacgcac tacaatagtg ctctgataat aggttataaa tgtacgtaaa
     7201 accattgttt caataaaaat gaaaacgtat acttcaagaa ggatgggtta cttaatataa
     7261 acaagggggt aacatatatg actttatatt tagatggtga aacactaaca attgaggata
     7321 ttaaatcatt tttacaacaa caatcaaaga ttgaaattat tgatgatgcg ttagaacgtg
     7381 tcaaaaaaag tagagcggta gttgaacgta ttattgaaaa tgaggaaacg gtttacggta
     7441 ttactacagg ttttgggtta tttagtgatg tacgtataga cccgacgcaa tataatgaat
     7501 tacaagtgaa tctgatacgc tcacatgcct gtggactagg tgagccattt tcaaaagaag
     7561 tagcattagt catgatgatt ttacgattga atacattatt aaaaggtcat tcaggtgcca
     7621 ctttagaatt agtgagacaa ttacaatttt ttataaatga acgtattata ccgataatcc
     7681 cacagcaagg ctctctcggt gcatcaggag atttagcgcc attatcacat ttagcattag
     7741 cattaattgg tgaagggaaa gtattgtaca gaggggaaga aaaggatagt gacgatgtat
     7801 taagagaatt aaatagacaa cctttgaacc ttcaggctaa agaaggttta gcattgatta
     7861 atggtacgca agctatgaca gctcaaggtg tcattagtta tatagaatca gaagatttag
     7921 gttaccaatc tgaatggatt gctgcattag cgcatcagtc tcttaatggc attatagatg
     7981 catatagaca tgatgtgcac gctgttcgta attttcaaga acagattaat gtggcagcgc
     8041 gtatgcgtga ttggttagaa ggatcaacat taacgacgcg acaagcagaa atacgtgtac
     8101 aagatgcata tacgttgcgt tgtataccac aaatccatgg cgcgagtttt caagtattca
     8161 attatgttaa acagcaatta gaatttgaaa tgaatgcggc taatgataat ccacttatat
     8221 ttgaggaagc aaatgaaacg tttgttattt caggtggtaa cttccatgga caacctattg
     8281 cttttgcatt agatcatctt aaattaggtg taagtgaatt agcaaacgta tcggaacgtc
     8341 gtttagagcg actagtaaat cctcaattaa atggtgattt accagcattt cttagtccag
     8401 agccaggatt gcaaagtggc gcgatgatta tgcaatatgc tgctgcaagt ctcgtttctg
     8461 aaaataaaac tttagcgcat ccagcgagtg ttgattctat cacttcatct gcgaaccaag
     8521 aagatcacgt atctatggga actacagctg ctagacatgg ttatcaaatt attgaaaatg
     8581 caagacgtgt gctggcaatc gaatgtgtta ttgcattaca agcagcagag ttaaaaggtg
     8641 ttgaaggttt atcaccaaaa acacgtcgta aatatgagga gtttcgaagt atcgtgccat
     8701 ccattacaca tgaccgtcaa tttcataaag atattgaagc ggttgcacag tatttaaagc
     8761 aatcaattta tcaaacgact gcatgtcact aaatcgacat gagcgatgtt tgaaatcgtt
     8821 acttgaaaaa gcaaatatag tttgctatat taaaaataac ttaataagac attgttcgta
     8881 ggacaagtaa tatatagtgt tcgatatcag agagcttgtg gttagtgtga acaagaatca
     8941 acatatatat gaatctacct acttaattta aaagaacaat cggtgataac cgttatttta
     9001 gtgaagtgca atttaggttt agtgtatctt tataacttaa attgttaaat agggtggcaa
     9061 cgcgtagacc acgtcccttg tagggatgtg gtcttttttt attttctaaa aaataaagac
     9121 gtacgtcatt ccataataaa tgataattat ttgggaaagg atgaaggtta atgttagaca
     9181 ttagattatt cagaaatgag cctgtcacag ttaagagcaa aattgaatta cgtggagatg
     9241 atccaaaagt tgtagatgaa attttagaat tggatgagca acgacgtaaa ttaattagtg
     9301 caacagaaga aatgaaagca cgtcgtaata aagtaagcga agaaatcgca ttaaaaaaac
     9361 gtaataaaga aaatgctgat gatgtgattg ctgaaatgcg cacattaggt gacgatatta
     9421 aagaaaaaga tagtcaatta aatgaaattg ataataaaat gacaggtatc ctttgtcgta
     9481 ttccaaattt aataagtgat gatgtacctc aaggtgaatc tgatgaagat aacgttgaag
     9541 ttaaaaagtg gggtacacca cgtgagtttt catttgaacc caaagcacat tgggatattg
     9601 tagaagaatt gaaaatggct gattttgatc gtgcagcaaa agtttcaggt gcgcgttttg
     9661 tatatttaac aaatgaaggt gcgcaattag agcgtgcttt aatgaactat atgattacaa
     9721 aacatacaac acaacatggt tatacagaaa tgatggtacc acagcttgtg aacgcagata
     9781 caatgtatgg tacaggtcaa ttacctaaat ttgaagaaga tttatttaaa gtagaaaaag
     9841 aaggattata tacaattcca actgctgaag taccattaac gaatttctac cgtaatgaaa
     9901 ttattcaacc aggtgtactt cctgaaaaat tcactggtca atctgcatgt ttccgtagtg
     9961 aagcaggatc agcaggtaga gatacaagag gattaattcg tttacatcaa ttcgataaag
    10021 tggaaatggt acgttttgaa caacctgaag attcatggaa tgctttagaa gaaatgacaa
    10081 caaacgcaga agcaattcta gaagagttag gtttaccata tcgtcgtgtt attttatgta
    10141 caggtgatat tggatttagt gcaagcaaaa catatgattt agaagtttgg ttaccaagct
    10201 acaatgatta taaagaaatt agttcatgct caaactgtac ggatttccaa gcgcgtcgtg
    10261 ctaacatccg cttcaagcgt gacaaagcag ctaaaccaga attagcacat acattaaatg
    10321 gtagtggttt agcagttgga cgtacatttg ctgctattgt tgaaaattac caaaatgaag
    10381 atggaacagt aacaattcca gaagcattag taccatttat gggtggtaaa acacaaattt
    10441 caaaaccagt taaataaagg ctttagctac aagctttaaa aagtatatat ctacgtatac
    10501 ttaaagcaag ggcaagatac tttaaataat attttaaaaa gtggtgacga agctgtcgcc
    10561 actttttttg tgctgtaaaa gtatagtagt gagccaatgc acataacaac aataaattaa
    10621 gtttgttgtt taatggggtg aacgcatttc attatagcaa caatacggga taattatgat
    10681 gaactaaaac aatctaaaac gtaacaattt tgagcatcac tattatagga aattaagcga
    10741 taaaatactg atttcgttga tgtagtatga gtaatatcga tggagtaggg tagggggagg
    10801 ggatgatgat tataagggag tggtacatga atcaa
//
LOCUS       DBNENW010000089        10830 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNENW010000089 DBNENW010000000
VERSION     DBNENW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.089"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            20..979
                     /locus_tag="AC1LW8_001786"
     CDS             20..979
                     /locus_tag="AC1LW8_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP4773613.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSSKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLLKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1025..1156)
                     /locus_tag="AC1LW8_001787"
     CDS             complement(1025..1156)
                     /locus_tag="AC1LW8_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAR1012 family small protein"
                     /protein_id="HIP4773614.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1220..1435)
                     /locus_tag="AC1LW8_001788"
     CDS             complement(1220..1435)
                     /locus_tag="AC1LW8_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIP4773615.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1600..2151
                     /locus_tag="AC1LW8_001789"
     CDS             1600..2151
                     /locus_tag="AC1LW8_001789"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773616.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVESKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESSTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2202..3140)
                     /locus_tag="AC1LW8_001790"
     CDS             complement(2202..3140)
                     /locus_tag="AC1LW8_001790"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIP4773617.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3291..3395
                     /locus_tag="AC1LW8_001791"
     CDS             3291..3395
                     /locus_tag="AC1LW8_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773618.1"
                     /translation="MKKDEERSEMDGYGRIRGRYCQRDIWQLKGMGFS"
     gene            3361..4683
                     /locus_tag="AC1LW8_001792"
     CDS             3361..4683
                     /locus_tag="AC1LW8_001792"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIP4773619.1"
                     /translation="MYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNR
                     TSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKS
                     DDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVD
                     EQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAY
                     SKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEA
                     FLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDS
                     YISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRS
                     MLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS"
     gene            4670..6343
                     /gene="menD"
                     /locus_tag="AC1LW8_001793"
     CDS             4670..6343
                     /gene="menD"
                     /locus_tag="AC1LW8_001793"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIP4773620.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DMTINRVSWLEKWQRLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHGTL"
     gene            6330..7133
                     /gene="menH"
                     /locus_tag="AC1LW8_001794"
     CDS             6330..7133
                     /gene="menH"
                     /locus_tag="AC1LW8_001794"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HIP4773621.1"
                     /translation="MAHYNYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7126..7947
                     /gene="menB"
                     /locus_tag="AC1LW8_001795"
     CDS             7126..7947
                     /gene="menB"
                     /locus_tag="AC1LW8_001795"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIP4773622.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVSE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(8185..8514)
                     /gene="sspC"
                     /locus_tag="AC1LW8_001796"
     CDS             complement(8185..8514)
                     /gene="sspC"
                     /locus_tag="AC1LW8_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HIP4773623.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(8552..9733)
                     /gene="sspB"
                     /locus_tag="AC1LW8_001797"
     CDS             complement(8552..9733)
                     /gene="sspB"
                     /locus_tag="AC1LW8_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HIP4773624.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSSKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(9815..10798)
                     /gene="sspA"
                     /locus_tag="AC1LW8_001798"
     CDS             complement(9815..10798)
                     /gene="sspA"
                     /locus_tag="AC1LW8_001798"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HIP4773625.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQSQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPNNPDNPDNGDNNNSDNPDAA"
BASE COUNT         3738 a         1560 c         2007 g         3525 t
ORIGIN      
        1 gttgtttgga ggtatatgtg tgaataggaa tatcgttaaa ctagttgtgt ttatgctaat
       61 cttagttgta gcagtagcgg gttgtggtca aaaagatact gaagagaaaa ctgaaatgac
      121 gacaataaaa gatgaattag gaactgaaaa aattaagaaa aatcctaaac gtgttgttgt
      181 attagaatat agttttgctg attatttagc agcattagat atgaaacctg ttggtattgc
      241 agatgatggc agcagtaaaa atataacaaa gtcagtaaga gataagattg gggcatatga
      301 atcggttgga tctagaccgc aaccgaatat ggaagtgata agtaaattaa aaccggattt
      361 gatcattgca gatgttagca gacataagaa aatcaaatca gaattgagca aaattgcgcc
      421 gacaattatg ttagtcagtg gtacgggaga ttacaatgca aatattgaag catttaaaac
      481 agtcgctaaa gcagttggca aagagaaaga aggcgagaag cgtctggaaa agcatgataa
      541 aatattagcg gagattagaa agaaaattga acagagtacg ttaaaatctg catttgcatt
      601 cggtatctca agggcaggta tgtttattaa taatgaagat acatttatgg gacaattctt
      661 acttaaaatg ggtattcaac ctgaagtcac aaaagacaaa actacgcatg ttggtgaacg
      721 caagggtggt ccttatatat atttaaataa tgaagaactt gccaatatca atccaaaagt
      781 tatgatttta gccactgacg ggaaaacgga caaaaataga acgaaattca ttgatcctgc
      841 agtttggaaa tcattaaaag ctgtgaaaga taacaaagtt tatgacgttg accgaaataa
      901 gtggttgaaa tcaaggggga ttatcgcaag tgaaagtatg gcagaagatt tagaaaaaat
      961 tgcagaaaaa gcaaaataaa aatacagcgc tactcgtaaa tcatataaga gtggcgctgt
     1021 atttttaatt atgtttatta tctgtcggat gtgatgattt acctgaaagt ttatttcgaa
     1081 taaatttaat tacataaccg acaaggattg ttttaacaat tcttttaatg aattggcgca
     1141 tcgttacata cctcatttct ctatatctta tgaactatat acccattcat atatgctttt
     1201 taaacgtcat tgtcactatt taatttttag ggaatataat ataaccatct ttatctgctt
     1261 ttttagtaaa aatgacaaaa atggcatgta ttattgagat gatggtaggg atacctgtcc
     1321 agaaaaataa taagtgaaaa agaccttgtc caaatttatc agcataaaat ttatgaatac
     1381 ctaaacctcc aagaaataat gcaacaataa cataaatggc tttattgact ttcatttgta
     1441 aatcctcctt aactataatt ctacttaaat tcgttgtgaa aaccaatatt tctaacttta
     1501 gaattttcaa actttctaaa attataagta tatcttttta aaataagcta gaatttctat
     1561 ataataaatg ttaataacgt aaaagggaat gatgacatag tgatacgtca agcacgtcca
     1621 gaggaccgat ttgatattgc gaagttagtt tatatggtgt gggatgatat ggaattagaa
     1681 ttggtaaagc atctacctaa agacatggta ttagatgcaa ttgaaaaaag ctgtgttgat
     1741 gcaacatatc gaacttttta tcagcatatt ttagtttatg aagtagaaag taaagtagca
     1801 ggttgtatta ttagctatag cggtgaaaat gaattgaaat atgaaaaagc atgggaacta
     1861 cttgacttgc cagaagaaat aaaacaatat ggcacgccat tacctgtaaa agaagctaaa
     1921 gacgatgagt attatataga aacaattgcg acatttgaag catatagagg tagaggcatc
     1981 gcgacaaagt tattaacgtc attacttgaa tcaagtacac atgttaaatg gagtttgaat
     2041 tgcgatatta ataatgaagc agcattaaag ttatataaaa aagtaggctt tatatctgat
     2101 ggacagattg aattatacaa gcacatgtat catcatttaa ttgttaaata aaaaactcga
     2161 cagttcgatg taagtcgatt gccgagtagt atcattttat attaaatgcc tgcaaataat
     2221 gcactaatat aaatacctaa tgcatataat aaaccgaaaa atgtatttgt tttaccagca
     2281 gcagccattg ctggcatcat tgtaggcggt gtatcattct tcttgaaacg tctgataact
     2341 ttaacaggca ttgggaatga taacaacgca agtaagtaaa ataatgagcc accaggttta
     2401 ataatgatcg taagtacaat aaaggcataa gcgataaagt acatgattgc cataaatgtt
     2461 aaagaagcat ttttacctaa tagaatgggt aaagttttgc gaccacttgc tttatctttg
     2521 acacggtcgc gaatattgtt agccatatta attaaaccga tagtgattac tataggtaca
     2581 cttaaccaaa ttacataact ttgaatattg ccagtttgaa taaagaatgc aataacgata
     2641 ataaacatac ccataaatac gcctgagaat aattcaccga aaggcgtcca tgaaataggg
     2701 aaaggaccac ctgtatatag gtaaccaaca gccatacata ctaatccaac tggtaataac
     2761 caaaatgaag agttagcagc taaaaacaaa cctaatattg ctgctaagat gtaaaatgca
     2821 atggctaatc gtagcacaag ctctgggctc ataccgttgc gaacaatggc accaccaatg
     2881 cctacagatt catgatcatc aaggcctttt ttataatcat agtattcatt aaacatatta
     2941 gttgctgctt gaataagtaa gcatgctagt aacatggcaa tgaataggct gattttaata
     3001 tgatcttcgc taccaagaaa atatatttta gatgctgctg taccaactaa aacgggtact
     3061 acggaagcag ttaatgtatg aggacgcatt aaatgccaat atttcttaac tgtagaatat
     3121 tgctgatatt gattactcat tgaatacaaa acctctttaa atcatatttt ttataacaaa
     3181 gatattttaa tagaaagtag cataaaggtc aattgacgtg aaaaattgca agataagttt
     3241 aagcaattct tgtaacacaa ttatccaaaa atgatacaat ataataatga gtgaaaaaag
     3301 acgaagaaag aagtgaaatg gatggctacg ggcgtattag aggacgatat tgtcaaagag
     3361 atatatggca gctcaaagga atgggtttca gttgaagtga aattatcaca gtcactagac
     3421 ccgagcacat tatttcatct cactgacaat gaggcaggag atcgctttta tatgcgtttg
     3481 aatgataatc gaacgtcata ttttggctac aaagcaattc aattattcaa aaataattct
     3541 aaaaataaac aatctatttt taaagactgg gaaaaattaa aacataacat cacatttata
     3601 catccgcaat ctgagaaaca tcatcttcga gttgttggag gattccaatt ttcgagtcat
     3661 aaatcagatg atgaatggcg agagtttgga ctaaatcatt ttgtattacc tgaagtttta
     3721 atttcaactg ataataatgg gacattttta acttatacag ttaaaaggga aagttttact
     3781 gttgaggcat tgaacgattt aatggatttg ttcaacaata tatcggacat agatgtggac
     3841 gagcaaattg gggaaattac tagaaatgaa gatatttata aagatgactg gcgtcaactt
     3901 gtagtagaag ctatagaatc tattaataat gaagaaaaaa ttgtactagc acgtagacgg
     3961 ttaataaagt tcgataaaga tatcagtatt ccatatattc taaagcaagc atattctaaa
     4021 gaaaaaaaca gttatatatt cttgttagaa tcacaagatt ctatattctt ttcacaaaca
     4081 cctgaacaat taataaaggt caataataaa atactatcga ctaaagctgt agcaggtaca
     4141 attaaacgtt cacaagatga ggacgaagat acaaaaaatg ttgaagcatt tttaaaagat
     4201 aataaaaact taatcgaaca tcgatttgtt gttgacagta ttttacatga tattaaacct
     4261 tatatcactg aattacatta tgataagacg cctaaaattc taaaaaatga tcatttatat
     4321 cacttgtaca ctgaaataaa ggcgccactg aaggatgatt cgtatattag tttaattgat
     4381 aatttacatc caacacctgc tttaggtggc tatccaaaag aatttgcgat ggattttatt
     4441 gaacagaaag aatttggtac acgaggatta tatggtgcgc cggttggcta tatagatata
     4501 tatgatgatt gtgaatttat tgttgcaatt cgttcgatgc ttattaagaa agcacaagca
     4561 actttatttg ctgggtgtgg cattgttaaa gattctgatc cagatagtga attggcagaa
     4621 acgaacctta agttcacacc tatgatgaat gcattaggag tcgatatgaa tgggaaatca
     4681 taaagcagct ttaacgaagc aagtttttac atttgcatct gagttatatg cgtacggcgt
     4741 aagggaagta gttatcagtc cgggatcacg ctcaacgcca cttgcacttg catttgaagc
     4801 acatccaaat attaaaacat ggatacaccc cgatgagcga agtgcagcat tttttgcagt
     4861 tgggttaatt aaaggtagtg aaagacctgt cgctatatta tgtacgtcag gtacagcagc
     4921 agcgaattat acgcctgcaa ttgctgaaag ccaaattagt agaattccat taatcgtttt
     4981 aacaagtgac cgtccgcatg aattaagaag tgtaggcgca ccacaagcga ttaatcaagt
     5041 aaatatgttt aataattatg taagttatga gttcgatatg cctattgcgg atgatagtaa
     5101 agagaccatt aatgcaattt attatcaaat gcaaattgct agtcaatatt tatatggacc
     5161 acataaaggg ccaattcatt ttaacttgcc atttagagat ccgttaacac ctgatttgaa
     5221 tgcaacagaa ttgttaactt ctgagatgaa gattttaccg cactatcaaa aaagtataga
     5281 tgcatcggca ttaagacaca ttttaaataa gaaaaaaggt ttaattattg taggggatat
     5341 gcagcaccaa gaagttgatc aaatactaac gtattcaacg atatatgatt tgcctatttt
     5401 agctgatcct ttaagtcatt taagaaaatt tgatcatccg aatgttatct gtacatatga
     5461 tttgctgttt agaagcggct tagacttaaa tgtggatttc gtaattcgtg ttgggaaacc
     5521 agtgatttct aaaaagttga atcaatggtt aaagaaaact gatgcatttc aaatattagt
     5581 gcaaaacaat gataagattg atgtctttcc gatagcacca gatatttcat atgagatttc
     5641 tgcgaatgat ttctttaggt cattaatgga agacatgacc atcaatcgcg taagttggtt
     5701 agaaaaatgg caacgcttag agaaaaaagg gcgtaaagaa attaaatgtt atttggaaca
     5761 agctacagat gagagtgcat tcgttggtga attgattaag aaaacatctg aaaaagatgc
     5821 attatttatt agtaatagta tgcctatcag agatgtagat aacttgttat tgaataaaaa
     5881 tatagatgtc tatgcgaatc gtggtgcgaa tggtattgat ggtatcgttt caactgcact
     5941 gggtatggct gtgcataaac gaataacatt attgataggt gatttatcat tttatcatga
     6001 tatgaatgga ctattaatgt caaaattaaa taatattcag atgaatattg tattattgaa
     6061 caacgatggt ggcggtattt tttcatattt accacaaaaa gaaagtgcaa ctgactattt
     6121 tgaacggttg tttggcacac cgacgggatt ggatttcgag tatacagcta agttatatca
     6181 attcgatttt aaacgtttta acagtgtttc agaatttaaa aatgccacat tgttatctga
     6241 aacttcgacg atttatgaat tgataacgaa tcgcgaagat aactttaaac agcatcaaat
     6301 tttatatcag aaattgagtg aaatgattca tggcacatta taattactat gaagcaaacg
     6361 ttgagaccaa tcaagtttta gtattactgc atggttttct tagcgacagt cgtacttatt
     6421 ataatcacat cgataaatat actgatatct gtcatgtcat cactatagac ttaccaggac
     6481 atggcgaaga tcagtcttca atggatgaaa cgtggaattt tgattatatt acgacgttgt
     6541 tagaccgaat tttagataaa tataaagata aatcaataac attgtttgga tattcaatgg
     6601 gtggacgtgt tgcattatat tatgcaatta atggtcacat ccctatatct aatttgatat
     6661 tagaaagtac gtcaccaggt attaaagaag aagcaaatca attggaacgc cgtcttgttg
     6721 atgatgcacg tgctaaagta ttagacatag caggtattga attatttgtt aatgattggg
     6781 aaaagttgcc attatttcaa tcgcaactag aattaccagt tgaaatacaa catcaaataa
     6841 gacaacaacg attgtcccaa tcgccacata aaatggccaa agcattaaga gattatggta
     6901 caggtcaaat gccaaactta tggccgcgcc tgaaagaaat taaagtacca acattaatat
     6961 tagctggaga atatgatgaa aaatttgtac agattgcgaa aaaaatggca aatttaattc
     7021 ctaatagtaa atgtaaatta atttctgcta caggtcatac aattcatgtg gaagatagtg
     7081 atgaatttga tacaatgata ttaggatttt taaaggagga gcaaaatgac taacagacaa
     7141 tgggaaacac ttagagaata tgatgaaatc aaatatgaat tttatgaagg gattgctaag
     7201 gtaacaataa atcgccctga agtacgcaat gcgtttacac ctaaaacagt ttctgaaatg
     7261 attgacgcat tttcacgtgc acgtgatgat caaaacgttt cagttatcgt attaactggt
     7321 gaaggtgatt tagcattctg ttctggtggt gaccagaaga aacgtggaca tggtggttat
     7381 gtaggtgaag accaaatccc tcgcttaaat gtattagatt tacagcgttt aattcgtatt
     7441 attccaaaac cggttatcgc gatggtaaaa ggttatgctg taggtggcgg taatgtacta
     7501 aatgttgttt gtgacttaac gattgctgct gataatgcta tttttggaca aactggtcct
     7561 aaagtaggtt catttgatgc gggttatggt tcaggatatt tagcacgtat cgttggacat
     7621 aagaaagcac gtgaaatttg gtacttatgt cgtcaataca atgcacaaga agctttagat
     7681 atgggtctag taaatacagt ggtaccttta gagaaagttg aagatgaaac tgtgcaatgg
     7741 tgtaaagaga ttatgaaaca ctcaccaaca gcgttacgat tccttaaagc agctatgaat
     7801 gctgacacag atggtttagc tggtttacaa caaatggctg gggatgcaac actgctttat
     7861 tacacaactg atgaagcgaa agaaggccgt gatgcgttta aagaaaaacg tgatcctgac
     7921 ttcgatcaat tccctaaatt cccataagtt attttgaata tgatattaag tcacttgcgt
     7981 cgtttattag cgacgatagg tggctttttt attttataag aatttaaaag gggcttttaa
     8041 ttaattatca taaatataag atagtttgag aaaattaata gagaaaaaat aaatgttaaa
     8101 gattattatt acaatcaaaa aacgagctgg tgagaaattg aattctcgcc aacccgcttt
     8161 atgaagtgaa atcatttaaa ttagttatac taagcgctca taaacgattg ggcgcgaagt
     8221 gccaatacct tgtttggatg acgtttgcat gaggatatgc tgtgtatcat cataatcaag
     8281 aatataaatg atttcttcat tatcaataga tgaaaattta atgcgttgat gtgccgtatc
     8341 tataaaaaga atatgatatt gattgtgact tgtatcatcg ccatgacgta tacaaattga
     8401 tttttgtaat tgatgattac tccaattacc aatgaataaa ttgatattgg tttgttctag
     8461 atgtgtgagt ttggttgtgt cgtaaactaa atttataaat tgtagttgat acatagtttt
     8521 acctgccttt aatcaatatc tatattactt tttagtaacc tatcattgaa ccataccagt
     8581 tataatcacg attgaatgat aaatgtaata ggcttgaatc tgcatcttgg attgataatt
     8641 ctgtatccca aggattccag taaataagtt tttcttggtc attaatttta gcattaccaa
     8701 caactgctag ggcatgtcct aaatgtggat cattagggtt ttgagataca ctttgtgcaa
     8761 gaatcataat tcctacatta tcttttgtaa gttgatcaac ttgttcatat gatggtacgc
     8821 cttcttgata atgaatatct ctgccttgtg atttaccgta ttcaatcatt tgattaggga
     8881 atgttgagca attaggaagg tcttgctcac ttacttcagg gtataatgta cgcataatat
     8941 catgtgcatt ataagtgtct gtatttttag ttgcatttaa taatgctgcc atactaaatc
     9001 ctgcacacca tgagttatcg aattgttgtt ctctaatttt gaagtttttt aatgtatttt
     9061 catattgaac ttgatcttct tgtatcgctt cgttttcttc aactttagta ggtgttactg
     9121 tattttttaa ctcttgtttc aattttgatg aaactgtttt agcactttct ttttctttta
     9181 cattaccagg tagtggcgta gcttttacta atctaacttt gccatcttct tcaaaataaa
     9241 atcctttttc atcagtaaga acagtgatat ttgaattttt atctttaatt tggtctaaat
     9301 ctttagcgat gaagtttgaa attttaacac tgtaattcat gtcttctttt gatttgttta
     9361 aatcatcttt atttttaggg ctaagtgtta aagtataaac aattttaccg tcttttataa
     9421 ctggataata gtaactatta tcttcttcac cattaaattt ataaatttta aaagcttcgc
     9481 caagttcata tttaccagtt tttgcattac tttgtttatc taatgcttta gcataaccag
     9541 caaattgttg ttgtgctaga tcttttactt tttgaggtac tttgtcactc ttaacattaa
     9601 tttctagctg tttagagtgt gaatcggctt tggctttatt gttattagca aatgcgccta
     9661 gtgataaaat aagcattgaa accattatga tgcttataat attgaatact ctagatttat
     9721 atgaactatt catataatac cctccattta aatcaaaata ttttgcgttg ctaagacttt
     9781 gcttctattt actaaatcta aattaagatg aagtttatgc agcgtcaggg ttgtctgaat
     9841 tattgttatc gccattgtct ggattatcag ggttgttagg gttgttaggg ttatcaggat
     9901 tgttagggtt atcaggatta tctgggttat taggttggtc atcgttggca aaatggatat
     9961 cttcaatatt ttgttttaag aagttgcgta cattttcatt aataaatacc gcaccattaa
    10021 attcatttgg tacaccgccc caatgaattc cgatcacttc atttttttca ttaaatacag
    10081 gtgaacctga gttaccacca gttgtactta aatcatattg catagcttcg cctttgaggt
    10141 aagtgatttt tcctttactt tcccacattg ttgctacagg tttatcacca ggatatcctg
    10201 ttacagtaat attttggtta acttgtgttt cagcattatt actcattgtt gctggtttaa
    10261 ctacttcacc aatatgtttg ttttgctcat taggggagaa tttaactatt gctaaatcac
    10321 cttcgcctga atatttagtg atttgttcag cagtgaaacc accatttgga taattgtctt
    10381 ggttaattgc agaagggaat gcttttaaag catgaggatc accgtgcgta gcatctacga
    10441 cgtgtttatt tgttaaaaga gtatctttac ctacaactac accggaagca ataaatgtac
    10501 cagtaggtgc ttcaacttga atataagtta cgggtgcata atgaccattc gttgtatctg
    10561 tgatttggtg acgatcgtta tttggtaata taacatttgc gtgttcacgt tgttctaatg
    10621 gtttaaggtt accgcctttt tgaatcttag gtgtttgctg tttgcttgac tgtgattgtt
    10681 gcggatgatt gtccatagcc tttgaagata acgcgtttgc tgctggagaa ctcacaagtg
    10741 tcgctgttgt caaagttgca acgaataaag aactaacttt taaaaattta cctttcatca
    10801 aaaacctcca aaaaatttat ttacaagtta
//
LOCUS       DBNENW010000090        10271 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNENW010000090 DBNENW010000000
VERSION     DBNENW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.090"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            118..1086
                     /gene="pfkA"
                     /locus_tag="AC1LW8_001799"
     CDS             118..1086
                     /gene="pfkA"
                     /locus_tag="AC1LW8_001799"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIP4773626.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            1108..2865
                     /gene="pyk"
                     /locus_tag="AC1LW8_001800"
     CDS             1108..2865
                     /gene="pyk"
                     /locus_tag="AC1LW8_001800"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIP4773627.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(3213..4574)
                     /locus_tag="AC1LW8_001801"
     CDS             complement(3213..4574)
                     /locus_tag="AC1LW8_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP4773628.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALSVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            4922..6043
                     /locus_tag="AC1LW8_001802"
     CDS             4922..6043
                     /locus_tag="AC1LW8_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIP4773629.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            6092..7360
                     /gene="icd"
                     /locus_tag="AC1LW8_001803"
     CDS             6092..7360
                     /gene="icd"
                     /locus_tag="AC1LW8_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIP4773630.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVEKEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            7750..8454
                     /locus_tag="AC1LW8_001804"
     CDS             7750..8454
                     /locus_tag="AC1LW8_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP4773631.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEVDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            8454..10115
                     /gene="pnpS"
                     /locus_tag="AC1LW8_001805"
     CDS             8454..10115
                     /gene="pnpS"
                     /locus_tag="AC1LW8_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HIP4773632.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         3679 a         1437 c         1986 g         3169 t
ORIGIN      
        1 aaaattcaat atttaaaacg tttacatcaa ttcaatacat tagttttgat ggaatgacat
       61 atcaatttgt ggtaatttag agttaaagat aaatcagtta tagaaaggta tgtcgtcatg
      121 aagaaaattg cagttttaac tagtggtgga gattcacctg gaatgaatgc tgccgtaaga
      181 gcagttgttc gtacagcaat ttacaatgaa attgaagttt atggtgtgta tcatggttac
      241 caaggattgt taaatgatga tattcataaa cttgaattag gatcagttgg ggatacgatt
      301 cagcgtggag gtacattctt gtattcagca agatgtccag agtttaagga gcaagaagta
      361 cgtaaagttg caatcgaaaa cttacgtaaa agagggattg agggccttgt agttattggt
      421 ggtgacggta gttatcgcgg tgcacaacgc atcagtgagg aatgcaaaga aattcaaact
      481 atcggtattc ctggtacgat tgacaatgat atcaatggta ctgattttac aattggattt
      541 gacacagcat taaatacgat tattggctta gtcgacaaaa ttagagacac tgcgtcaagt
      601 cacgcacgaa catttatcat tgaagcaatg ggccgtgatt gtggagatct agcattatgg
      661 gctggattat cagttggtgc tgagacaatt gtagttccag aagtgaaaac agatattaaa
      721 gaaatagctg ataaaattga acaaggtatt aaacgtggta agaaacactc aatcgttctt
      781 gtagcagaag gttgtatgac tgcgcaagat tgtcaaaaag aattatcaca atacatcaat
      841 gttgataata gagtgtctgt gttaggtcac gttcaacgtg gtggtagccc aacaggtgcg
      901 gatagagttt tagcatcacg tttaggtgga tatgcggtag acttattaat gcaaggtgaa
      961 acagctaagg gtgttggaat taagaacaat aaaattgtag caacatcttt tgatgaaatt
     1021 tttgatggta aagatcataa atttgattat agtctatatg aacttgctaa caagttatct
     1081 atataagatt tcaggaggaa ttataaaatg agaaaaacta aaattgtatg tacaattgga
     1141 ccagcttcag aatcagaaga aatgattgag aaattaatca atgctggtat gaacgttgca
     1201 cgattaaact tttcacatgg tagtcatgaa gagcataaag gtagaattga tacaattcgt
     1261 aaagtagcta aaagattaga caaaattgta gcaattttat tagatacaaa aggtccagaa
     1321 attcgtacgc ataatatgaa agacggtatc attgaacttg aacgtggtaa cgaagttatt
     1381 gttagcatga atgaagttga aggaacacct gaaaagttct cagtaacata tgaaaactta
     1441 attaacgatg ttcaagtagg ttcatacatt ttacttgatg atggcttaat tgaattacaa
     1501 gttaaagata ttgaccatgc taaaaaagaa gttaaatgtg atattttaaa ctctggtgag
     1561 cttaaaaaca aaaaaggtgt taacttacct ggcgtaagag taagtttacc tggtattaca
     1621 gaaaaagatg ctgaagatat ccgtttcggt attaaagaaa atgttgactt cattgcagca
     1681 agtttcgtac gtcgtcctag tgatgtttta gaaattcgtg aaattttaga agaacaaaaa
     1741 gctaacattt cagtattccc taaaattgaa aaccaagaag gtattgataa tattgcggaa
     1801 attcttgaag tgtctgatgg tttaatggtt gcacgtggtg acatgggtgt tgaaattcca
     1861 cctgaaaaag taccaatggt tcaaaaagat ttaatcagac aatgtaacaa attaggtaaa
     1921 ccagttatta cagctacaca aatgttagat tctatgcaac gtaacccacg tgctacacgt
     1981 gcagaagcta gtgacgttgc caacgcaatc tatgatggta cagatgcagt aatgttatct
     2041 ggtgaaactg ctgctggttt atatcctgaa gaagctgtta aaacaatgag aaatattgct
     2101 gtatcagctg aagcagccca agattacaaa aagttattgt cagatcgtac taaattagtt
     2161 gaaacttcat tagtgaatgc tatcggtatt tcggttgcac atacagcttt aaacttaaat
     2221 gttaaagcaa ttgtagctgc tactgaaagt ggttcaacgg cacgtactat ctctaaatat
     2281 cgtccgcatt cagacattat tgcagtgact ccaagtgaag aaactgcacg tcaatgttca
     2341 attgtttggg gagttcaacc tgtagttaaa aaaggacgta agagtacaga tgcattgtta
     2401 aacaatgcag ttgcaacagc tgttgaaact ggtagagtat ctaatggtga tttaatcatt
     2461 attactgctg gtgtaccaac tggtgaaact ggaactacta atatgatgaa aatccaccta
     2521 gttggtgacg aaattgctaa tggtcaaggt attggacgtg gatcagttgt tggtactacg
     2581 ttagttgctg aaactgttaa agatttagaa ggtaaagatt tatctgacaa agttatcgtt
     2641 actaactcaa tcgatgaaac gtttgtacct tatgtagaaa aagctttagg cttaattaca
     2701 gaagaaaatg gtattacatc accaagtgca attgttggtt tagaaaaagg tattccaaca
     2761 gttgtaggtg tagaaaaagc tgttaaaaac ataagcaata acatgttagt tacgattgat
     2821 gctgctcaag gtaaaatctt tgaaggatat gcaaacgtac tataatttat aaaaaaacgt
     2881 ctttccattt atcaacaatg gaaaggcgtt ttttggttca tctggtattt tatgacgtaa
     2941 ttaataggtt atttgataat gatagtgtat gaatggcaat ctatataaat gtttatatct
     3001 tttatacatg tacattatca ccttcaaact ttcactcata ttactttgga aatttattat
     3061 aaaatagaag tatggatgta tttctgaaat gatacattat taaatagatg agaaagtaaa
     3121 agttttgagc caagtacgca atttaatatt ataagttgca tataaaaacc cctgttgaac
     3181 tttagtatat tcaacagggg taaaacttgt tgttatttat ttgatttagc agcgatacgt
     3241 ttatatctta agtacataaa tgctaaaagt ataaaccaaa tcggaataaa ataaattgca
     3301 cgtcttgtat caacattaat aaataataac ccgaacacaa aaatgaagaa tacaaatatt
     3361 aagtagccca tatatttacc acctaatagt ttgtacgtag catttttatg tagatctggg
     3421 tttttacgac tataattgat atatgcaatg ataatcagac cccatacaac taaaaataac
     3481 actgtagaga tggtagtcac atacgtaaat acttttgtcg catctgggaa aatatagttt
     3541 agtaatgctg ccacaagtaa taatgctgat gaagcaaaga ttgcaacatg tggaacgcca
     3601 tatttattcg tcttagaaaa gttcggaggt gcttgttgtt gacttgataa accgaaaagc
     3661 atacggctat ttgagaatat accactatta catgatgaag cagcagcggt taatactaca
     3721 aaattaatca agcccgcagc aaacggaata ccgatcaatg cgaataattt tacgaatgga
     3781 ctgttatcag gatcaacttg ctgccaaggg ataatagaca tgataaccga taacgcccca
     3841 acgtaaaata ttaaaattct aatgggtaca ctgttaattg cttttggaat tgttttaact
     3901 ggatcttttg tttccccagc tgtaacacca atcatttcaa ttcctacaaa tgagaatagt
     3961 gccatttgga aagacataaa gaatccagaa gcacctttag cgaatatgcc gtgttcatat
     4021 aaatttgtga aactggcatg cccgaattga gttttaaatg caaatagaat catgacgaaa
     4081 ccaactacta ttaaaccaat aattgtcgct attttaatga tagagaacca aaattctaat
     4141 tctccgaaaa gtcttgcgct aagtaggttg aatgacatta ataacagtac acaaaatagt
     4201 gcacttatcc agtttggaat ttctgggaac caaaagctaa catattttgc cacagccgtt
     4261 acttcagcca tacctgtaat aatccaacag aaccagtatg tccatccggt aacaaatcct
     4321 gcaaaaggcc caatatatgt attggttaca tctgcgaaag atttaaattc agtattctgt
     4381 ataatgattt ctcctaaacc tcgcataaac ataaataaca taaatcctat aatgatgtat
     4441 gttaatagaa ttgaagggcc ggttaatgca atcgtttgac cagcacctaa gaataagcct
     4501 gtaccaattg caccgccaat tgcaattaat tgtatgtggc gattgctcag ttccctttgt
     4561 aatttttcag ccataataca tctcccttaa atatagatat gtttattatg cacttatatt
     4621 gagatatata caattatttt cggtaaaaat gtgtaaaatt ccatgttaat atactttggt
     4681 ttttataatc atatataata accaattgaa aatttaattc tattgtaaaa ttcatggatt
     4741 attcacatct tgaaaaagct ttaatggtgc tatttgtggc tattctgtga catttacata
     4801 gatttacaaa aaaattgttg cacatataat gccagttttt atatttcaca aacgaaatgc
     4861 gtttagctat aatattagtt gaaagccatt tcataaagaa acagtaaagg ggaaatttat
     4921 catggcagaa ttacaaagag gtttagaagg ggttatcgca gcggagacta aaataagttc
     4981 aattattgaa agtcaattga cttatgccgg ctatgatatt gatgatctag ctgaaaatgc
     5041 gcaatttgaa gaagttattt tcctattatg gaactataga ttgccaaacg aagaagagct
     5101 tgctcatcta aaagggaaat taaatcaata catgacatta aatcctcgtg tgtatacaca
     5161 ttttgaggag tatgttacag atcacgtgca tccaatgaca gcattacgta cgtcattatc
     5221 atatattgca catttcgatc ctgatgctga aaatgaatca gatgaaaatc gttatgaaag
     5281 agcaatgcgt atacaggcta aagtagcatc attagttaca gcgtttgctc gagtaagaca
     5341 agataaagaa ccacttaagc ctaatcctga cttaagttat gcggcaaact tcctatatat
     5401 gttacgtggg gaattaccaa cagatataga agtagaagcc ttcaataaag cacttatttt
     5461 acacgctgat catgagttga acgcatctgc atttacagca cgttgtgcgg tatcatcatt
     5521 gtcagatatg tactcaggta ttgtagcagc tgtaggttct ctgaaagggc cattacatgg
     5581 tggtgcaaac gaacaagtta tgacgatgtt atctgagatt gggtcaattg aaaatgttga
     5641 tgcttactta gatgaaaaat ttgctaataa agataaagta atgggcttcg gtcatcgtgt
     5701 atataaagat ggtgatccta gagcgaaata tttaagagaa atgagccgtc aaattacgaa
     5761 agacgctggt cgtgaagaat tatttgaaat gtcagtgaaa atggaaaaac gtatggcaga
     5821 agaaaaagga ttaattccta atgttgattt ttatagtgcg agtgtttatc actgtatgga
     5881 aatacctcat gacttattca cgccaatctt tgctgtaagt cgttctgcag gatggattgc
     5941 tcatatttta gaacaatata aagataatag aattatgcgt cctagagcga aatatattgg
     6001 cgaaacgaat cgtaagtata tcccgcttga agaaagaaaa taatcaatac aaattaaaaa
     6061 tgaagatgta aaatttggag gtaaaataac tatgactgca gaaaaaatta ctcaaggaac
     6121 tgaaggatta aacgtaccta atgaaccaat tatcccattt attatcggtg atggaattgg
     6181 accggatatt tggaaggcag caagccgagt tatagatgct gctgttgaga aagcctataa
     6241 tggcgaaaaa cgcattgaat ggaaagaagt gctagctggc caaaaagcat ttgatacaac
     6301 tggtgaatgg ttacctcaag aaacacttga tacaattaaa gaatatttaa ttgctgttaa
     6361 aggaccttta acaacaccaa ttggtggtgg tattagatca ttaaatgtgg ctttacgcca
     6421 agaattagat ttatttactt gtttaagacc ggtacgttgg tttaaaggag taccatcacc
     6481 tgttaaacgt ccacaagatg ttgatatggt tattttccgt gaaaatactg aagacattta
     6541 tgctggtatt gaatttaaag aaggtacaac agaagttaaa aaggtaattg acttcttaca
     6601 aaacgaaatg ggtgcgacaa atattcgatt cccagaaact tcaggtattg gtattaaacc
     6661 agtttctaaa gaaggaactg agcgattagt tagagcagct atacaatatg ctatcgataa
     6721 taaccgtaaa tcagttactt tagttcataa aggtaatatt atgaaattta cagaaggctc
     6781 atttaagcag tggggttacg atttagcatt atctgaattt ggtgatcaag tattcacttg
     6841 gcaacaatat gacgaaattg ttgaaaaaga aggcagagat gctgctaatg ctgctcaaga
     6901 aaaagctgaa aaagaaggca agattatcat taaagattct attgctgaca ttttcttaca
     6961 acaaatttta actcgtccag ctgagcatga tgttgtagca actatgaact tgaatggtga
     7021 ctatatttca gatgctttag ctgcacaagt tggtggtatt ggtattgcgc caggtgcaaa
     7081 cattaattat gaaacaggtc atgctatttt tgaagcaaca catggtacag ctccaaaata
     7141 tgcaggttta aataaagtga atccatcttc agtaatttta agttctgtat taatgttaga
     7201 acatttagga tggcaagaag cggcagataa gattacagat tcaattgaag atacaattgc
     7261 ttcaaaagtt gttacttatg actttgcccg tttaatggat ggtgctgaag aagtttctac
     7321 atcagcattt gcagatgaat tgattaaaaa tttaaaataa gcaaaataaa aataggtgag
     7381 tctagcaact ttaattgcta ggttcgcctt tttaaatgaa ttgaagttgg caacaggata
     7441 gaaataataa agagtaacta atgattgatt atatagtgtt tcttacacat tagacaaagc
     7501 gattgtgtac ataagcttag gaataaatga gtaaaactca tgcataggta gtggcataga
     7561 aatgatattt tcaaaaagtt tatttcgttg tcccagagta agtcatgcaa atgatagaag
     7621 gaaatataag aagtatttta tttatatgta aagtaatgta aaaaatgtgt aaagataatg
     7681 tgtaggatgt tatttttata aatagttcgg ttataatgta aatataatta taagttaggg
     7741 aggcatacca tgtcgcaaaa agtgttggta gtagatgacg aacattcaat cgtaacatta
     7801 cttaaatata acttagaaac agctggttat gaagttgttg tcgcatttga tggtgatgag
     7861 gctttagaaa aggtagaaag tgaacagcca gatttaatta ttttagatgt tatgctacct
     7921 aaaaaagatg gcattgacgt atgtaagact gtaagaacaa ataaaaattt agtccctata
     7981 ttaatgttaa cggcgaaaaa tgatgaattt gatcgggtat taggtttaga attaggtgcc
     8041 gatgactata tgactaagcc tttttcacct agagaagttg ttgcccgtgt gaaagccatt
     8101 ttaagacgtt ctcaatttgt aaaagaagta gaagaggttg atgatgatat taccatcgga
     8161 tcgatacgta ttagacccga ttattttgaa gtatataagc ataatgagtt attggagcta
     8221 acaccgaaag aatttgaact cttgctttat ttgatagaaa gacaaggcag agtcattacg
     8281 agagaacata tgttaaatac ggtatggaat tatgaatttg caggagactc aagaattgtc
     8341 gatgtacata tcagtcattt aagagataaa ttagaagata atcctaagaa gccacaactt
     8401 attaaaactg tgcgtggctt aggctataaa ttggaaagac ctaaagaaca atgatgaagt
     8461 ttcaccaccg cttaatgtta ttgataagca gtatattaat tatcagtttt gttacattag
     8521 gcattattat tcacgcttcc atcacatatg ttttatcaga aaaacacgaa caagctctag
     8581 caacagatgc aagatcatat atttatttag tccaagataa tgagattgat aaagttaaag
     8641 aaattgtaaa acagcaaaat atagatttat tgataactaa gcatgataaa aaggtgttta
     8701 gtagtggcaa actgaaagct atcccaaata gtgatagcta tattcaaaaa agggacgttt
     8761 ttatttttaa taagaaggtt aacggttatc atgtatggat taaaggttac aataacgata
     8821 ttacagaaat gcattggacg ttgtggaaat atcttatttt gacatgttta gttgtactca
     8881 tttgtctgta ttttgcaagc cgtagtttta agcgtacatt aatccgtccc attcaagaag
     8941 ttacttatgc aacacaattg ttggcaaatg gttattatca catacgtgta cctgaaagta
     9001 atgttgtaga aactaaagcg ctttttgtgt ctaccaatga tttagcacga cgccttcaaa
     9061 agcttaataa tgagcaaaaa attcaatcca accgtttaaa aacaacgatt gaaaatattc
     9121 caagtgctat tttaatgata gatcgaaatg gaaaaatcgt tgttgcaaat aaagcgtatt
     9181 atgaacagtt caacatctcg cataatattg aacaagtagg ctatcacggt tatgttaata
     9241 ctgaaattga gcaattaatt ttagaaagtt ttaaagttga aaaacctatt tatgaacaat
     9301 tggaagttgc aataaaccaa gtccatgcca agtactttga tatttcatgc gttccgattt
     9361 taactaggtc tcaaaaaagt ttgcaaggga ttttagttgt gatgcatgac ataacaaatc
     9421 tgaaacaact tgaaaatcta agacgtgagt ttgtagcaaa tgtttcacat gaattaaaaa
     9481 cacctattac ttcaattaag gggtttgctg aaacgcttat tgatggtgca aaaaatgatg
     9541 cggaatcatt agacatgttt ttaaacataa ttttaaaaga atcaaatcga atagaatctt
     9601 tagtaacaga tctgttagat ttatcgcata ttgagcaaca tacagagtta gatacagact
     9661 atatgaattt atcagattta acgcggcgta ttattgataa tatgatgact caagccaatc
     9721 aaaaaaatat ttccattcat actgatattg aaaaagatgt cattgttaaa gcacaagaaa
     9781 gtaaaattgc tcaagttatc acgaatttgt taacgaatgc aattaactat tcttatgaag
     9841 atggagatat taacgttcgt gtgtatcgag atgactttcg tgtcattttc gaagtacaag
     9901 attttggtat aggtattaaa ttagaagacc aacaacgtat ttttgaaaga ttttatagag
     9961 tagataaagc acgaagtaga gattctggtg ggacaggtct tggattatca attacaaagc
    10021 atatagtaga ggcccaccaa ggcaatattg aagtgaatag tcaagttggc aaaggctcaa
    10081 cgttcaaagt tattctaaaa gattataaag aataaaaatt aaagtggtaa cagcgcgtgt
    10141 atttttcacg aagttgttgc tgctttttta tttcgttcaa atctctattg gtattaaatt
    10201 agagttaatg aagtggaata aattcggttg tcaattttgt catttgtata tgtcgatttg
    10261 taagttataa g
//
LOCUS       DBNENW010000091        10271 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNENW010000091 DBNENW010000000
VERSION     DBNENW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.091"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1413
                     /gene="coa"
                     /locus_tag="AC1LW8_001806"
     CDS             <1..1413
                     /gene="coa"
                     /locus_tag="AC1LW8_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HIP4773633.1"
                     /translation="GDLTMEEYNDLSKLLTKALDNFKLEVKKIESENPDLKPYSESEE
                     RTAYGKIDSLVDQAYSVYFAYVTDAQHKTEALNLRAKIDLILGDEKDPIRVTNQRTEK
                     EMIKDLESIIDDFFIETKLNRPKHITRYDGTKHDYHKHKDGFDALVKETREAVAKADE
                     SWKNKTVKKYEETVTKSPVVKEEKKVEEPQSPKFDNQQEVKITVDKAEETTQPVAQPL
                     VKIPQGTITGEIVKGPEYPTMENKTLQGEIVQGPDFPTMEQNRPSLSDNYTQPTTPNP
                     ILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQASETTEASHYPARPQFNKTP
                     KYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTNQDGTVTYGARPTQNKP
                     SKTNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSKTN
                     AYNVTTHADGTATYGPRVTK"
     gene            complement(1595..1702)
                     /locus_tag="AC1LW8_001807"
     CDS             complement(1595..1702)
                     /locus_tag="AC1LW8_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773634.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(2016..3200)
                     /locus_tag="AC1LW8_001808"
     CDS             complement(2016..3200)
                     /locus_tag="AC1LW8_001808"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIP4773635.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALLTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYEAQRDGRFNDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(3230..5491)
                     /locus_tag="AC1LW8_001809"
     CDS             complement(3230..5491)
                     /locus_tag="AC1LW8_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIP4773636.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQTFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDEIGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(5678..6889)
                     /locus_tag="AC1LW8_001810"
     CDS             complement(5678..6889)
                     /locus_tag="AC1LW8_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIP4773637.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(7001..8506)
                     /locus_tag="AC1LW8_001811"
     CDS             complement(7001..8506)
                     /locus_tag="AC1LW8_001811"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIP4773638.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYVLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(8532..10109)
                     /locus_tag="AC1LW8_001812"
     CDS             complement(8532..10109)
                     /locus_tag="AC1LW8_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIP4773639.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDGYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTERAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
BASE COUNT         3259 a         2035 c         1778 g         3199 t
ORIGIN      
        1 ggcgatttaa ctatggaaga atacaatgac ttatcaaaat tattaacaaa agcattggat
       61 aactttaagt tagaagtaaa gaaaattgaa tcagagaatc cagatttaaa accatattct
      121 gaaagcgaag aaagaacagc atatggtaaa atagattcac ttgttgatca agcatatagt
      181 gtatattttg cctacgttac agatgcacaa cataaaacag aagcattaaa tcttagggcg
      241 aaaattgatt tgattttagg tgatgaaaaa gatccaatta gagttacgaa tcaacgtact
      301 gaaaaagaaa tgattaaaga tttagaatct attattgatg atttcttcat tgaaaccaag
      361 ttgaatagac ctaaacacat tactaggtat gatggaacta aacatgatta ccataaacat
      421 aaagatggat ttgatgctct agttaaagaa acaagagaag cggttgcaaa ggctgacgaa
      481 tcttggaaaa ataaaactgt caaaaaatac gaggaaactg taacaaaatc tccagttgta
      541 aaagaagaga agaaagttga agaacctcaa tcacctaaat ttgataacca acaagaggtt
      601 aaaattacag ttgataaagc tgaagaaaca acacaaccag tggcacagcc attagttaaa
      661 attccacagg gcacaattac aggtgaaatt gtaaaaggtc cggaatatcc aacgatggaa
      721 aataaaacgt tacaaggtga aatcgttcaa ggtccagatt tcccaacaat ggaacaaaac
      781 agaccatctt taagcgataa ttatactcaa ccgacgacac cgaaccctat tttagaaggt
      841 cttgaaggta gctcatctaa acttgaaata aaaccacaag gtactgaatc aacgttaaaa
      901 ggtactcaag gagaatcaag tgatattgaa gttaaacctc aagcatctga aacaacagaa
      961 gcatcacatt atccagcaag acctcaattt aacaaaacac ctaaatatgt taaatataga
     1021 gatgctggta caggtatccg tgaatacaac gatggaacat ttggatatga agcgagacca
     1081 agattcaata agccatcaga aacaaacgca tacaacgtaa cgacaaatca agatggcaca
     1141 gtaacatatg gcgctcgccc aacacaaaac aaaccaagca aaacaaatgc atacaacgta
     1201 acaacacatg caaatggtca agtatcatat ggcgctcgcc cgacacaaaa caagccaagc
     1261 aaaacaaatg catataacgt aacaacacac gcaaacggtc aagtgtcata cggagctcgc
     1321 ccgacataca agaagccaag taaaacaaat gcatacaatg taacaacaca tgcagatggt
     1381 actgcgacat atgggcctag agtaacaaaa taagtttgta actctatcca aagacataca
     1441 gtcaatacaa aacattacgt atctttacaa cagtaatcat gcattctatg atgcttctaa
     1501 ctgaattaaa gcatcgaaca atcggaagca tatttctaaa ttatttattc attatagtct
     1561 taaacataac atgacctaat atattactaa cctattaaaa taaaccacgc acatctaagt
     1621 gatatacgac aatcacagca ataataattg ctttagaaag tcgtgccgaa ctggaactta
     1681 caagtctagt tcgaacacac actgatgtga gtggttttct ttattttaaa catgaacaat
     1741 cagataagtt actagcatta gcaaatatta ttaaatcaaa gggcttcgat tcataaaatt
     1801 taaaacaatg attaaaatta gacgtgtaaa tgttaaattc taaaacggaa ataaccacca
     1861 tcccattaaa ccactttttt gttcaatcac tatatttcac acagcttcat taataaaacg
     1921 aaattgcttc aacccgcttc aacttcaact ggcttcaact tcagcctact tcattcaata
     1981 acaaaacgaa tccgcttcat ccaaaatcaa ccattctaac gcacatattc aaatatagca
     2041 gctgcaccca taccgacacc aatacacatc gtaaccatgc cgtaacggct atcgggacgt
     2101 ctacccattt cattaagtaa acgcgcggtt aacattgcgc ctgtagcacc taatggatga
     2161 cctaaagcaa tagcgccacc attcacattc gtacgtgata tatctagacc tacttcttta
     2221 atagatgcaa ttgtttgaga agcaaatgct tcgttcaatt cgatcaaatc aatgtcttca
     2281 acagatagat tgctgagtga caatacttca ggaatcgcat atgcaggccc aatacccata
     2341 attttcgggt caacgcctac tgccttataa ccaacgaatc gtgcaatagg tgtcacgcct
     2401 agttctttca ctttatctcc agacattaaa actacaaatc ctgcaccatc agaaagtggg
     2461 gcagatgttc ccgcagtcac agtgccgtca gctttaaata ctgtacgtaa tttggctaat
     2521 gcctccatcg tggtgtcagg gcgtataaat tcatcttggt caaagatatt tgtgtgtact
     2581 tttggtcctg cgtttgtata ttcaactgag tttacttgta ttgggataat ttcatcgttg
     2641 aaccgaccat cacgttgtgc ttcataggca cgttgatgac ttctgacagc ataagcatct
     2701 tgatcttcgc gtgatacgtc aaattgggat gctacatttt cagcagttaa acccatagga
     2761 tatgacgcac ctatatcatc atattgtaag gtaggattgt ttgtgggctc gttgccaccc
     2821 attggtacgg cactcatcaa ttcaacgcca ccagctacaa gtatatctcc ttgaccagcc
     2881 ataatttgat tggctgcaat cgcgatggtt tgtaatcctg atgagcagta gcgattcact
     2941 gtttgacccg gtaccgtgtc agataatccc gtaagcaatg caatcgttcg tgcaatgttt
     3001 tgtccttgta atccttctgg aaaagccgta ccaacaatga catcttcaat catattctta
     3061 ttgaattttc cgtcaatacg tttcaatacg ccttgtaata ctttggctgc gacatcatca
     3121 ggtctttcgt ggaataatgc gccttgcttt gctttcgctg cggctgaacg cccataagct
     3181 acaatgtatg cttcttgcat ggttatcatc ctctcttaat gactatcttt taattacgta
     3241 atggcttacc agtttttaac atatgtgcaa ttctttcata tgatttttta gattttagta
     3301 agtcaataaa gccaattttc tccaacgatt gaatgtaacg ttgattgata aatgtatttc
     3361 ttggtaaatc accacccgct aaaattgtgg cgatatttaa ggcgatatga taatcatggt
     3421 cgctaataaa atgaccccgt ctttgcgcat ctaattgtcc ttggatcaat gctttgaagt
     3481 cttcacctaa agcgatatat tgatgtctag gattcggaat atagtttgtt tctgcttcat
     3541 atttcgcacg tttgagcgca acttcgacac gttgtgctgt attgaaaata atcgtatctg
     3601 tatcacgtaa ataaccataa cgacgtgcct caaaggcatt tgtagagact ttcgcaaatg
     3661 cgatattcgt cagtactttt gtcatggaag cttgtttgtc atcaaactta tgcgatgtgc
     3721 gtaatatgcg atcagccatt tctgcaaggc caccgccact cggtaataag ccaacgcctg
     3781 cttcaacaag accgatatac gtttcacttg cagcgacaac aataggtgag taaagtacaa
     3841 gctcacagcc accgcctaag gcacgacctt gaacagctgt gactactggt ttcaaactat
     3901 acttcaaacg attaaagcta taatgtaatt tatcaattga ttgtgcaacg acatcatcta
     3961 caagaccgtc ttcatgcgcc tttttcatta agaaaaggtt agcacccaca ctgaaattgt
     4021 taccatctgc ataaataacc atacttgtgt aatggtcatt ttccagtaaa tcaatcgcat
     4081 caactaacgc atcgttgaat tcatcggtaa tgacattatt tttactttgt aatttcagta
     4141 acagttgatc atcatgagtt acggaaagtt tggcatcacc tttatcccaa agttcatctt
     4201 ttacgaagtg agaaacaggt gttgcatatt cgatggtctc atcttgttta taaaagccac
     4261 catctaaatc actaatccat tgtggtaagt ctccaatttc gtcttccata cgtgttttaa
     4321 cacgttcata tcccattgca tcccataatt ggaatggacc aagtttccag ttgaaccccc
     4381 agacaagcgc acggtctatg tctcggaaat catcggtagc tttaggtaca ttgatagcag
     4441 agtaatagaa attattacgt aatgtctccc ataaaaatag tcccgcttcg tcttgcgcat
     4501 tgaatatgac atcaaggtta tgcactaagt ctttattaaa ttcatttaaa attggtaatt
     4561 gtggttgcga tacaggtaca taatcttgtt tttcaacatc gtaaacaagt cgagctttag
     4621 tttctttatc ctttttgtaa aatccttgtt tcgttttacg tccgagtgcg ccattgtcaa
     4681 acaacgtatt tacaattttg acatcatgaa aataaggcgt ttcttcaggt acttgttgca
     4741 tgcctttaat tacagacact gcaatatcta aaccgactag gtcagatagg gcatatgtac
     4801 ctgttttagg acgaccaatc gcttgcccag ttaaagcatc cacatctaca atgcttaact
     4861 tgtgttgctc ggcgcgatac ataatatcat tcattgtttg cgtgccgact ctatttgcga
     4921 caaagccagg cacatcattg acgacaatga cacctttacc taacacattt tgcgcgaaat
     4981 tttttacatc taatatgata gattccttcg tgtgtgacgt aggtattaac tccactaatt
     5041 tcataatacg tggtgggtta aagaaatgta gaccaaagaa tcgttcttga tccttctcgt
     5101 taaatgtttg agcaatcgca ttaattggaa tacctgatgt atttgtagcg aataaagcat
     5161 cttctttagc atgttgtaga acttgttgcc aaacagcatg cttaatttca atatcttctt
     5221 taactgcttc gatatataaa tcagcatcat catttaccaa gtcatcatca aaattaccat
     5281 atgttaaatg actcgctaga tttaagtcga atagtagcgg ccgtttctta tctgtaattt
     5341 tatcgtaaga ttttttcgca atgagatttg gatcgttttt gtccactaca atatctaata
     5401 gttttacttt aagtccagca ttcacaaaga gtgctgccag ttgagcgccc atcgtgcctg
     5461 cgccaagaac ggttacttta ttaattgtca tagtgattcc tccaatttag ttgaggataa
     5521 gataaccatt aagataattg gaataacgtt gctattttat aaaattaatt aagtatcttt
     5581 gacagtcatc ttagcctctt atttaaggaa aaagctttat gcttaaaata agtctttttt
     5641 tagtgatatt actgcatctc atataattct ttgctattta tacgaaagca gaatctccag
     5701 tcaaagcgcg tccaattact aaggcattaa tttcatgtgt accttcgtat gtgtaaatcg
     5761 cttctgcatc agagaagaaa cgtgcaatat cataatcgtc agctagtatg ccattaccac
     5821 ctgtaatacc acggcccata gctactgtct cacgcaaacg taaggcattc atcatcttcg
     5881 ccgttgaagt tgcaacctcg tcatattcac catgtgcttg catattagct aattgagcac
     5941 atgttgccat tgcttgagct aaattacctt gcatcattgc tagcttttct tgtattaact
     6001 gatatttact aattggtttg ccgaattgct tacgctcagt gacataatct aatgtggcac
     6061 gtaaagcgcc agccatacca cctgtagcca tataagcaac gcctgctctc gttgaataaa
     6121 gaattttggc aatatcttta aagcttgtta tgttttgtaa gcgatccgct tcatctactt
     6181 tgacattagt taatttaatt agggcgttag gaacaatgcg aagtgcgatt ttattatcaa
     6241 tgacttcaat atcgacgcca tcttgttctg gtctgactac aaagcaatgg ggtttgccag
     6301 tttctttatt tactgcgaat actggaatga catcagatac atgtgcacca ccaatccatt
     6361 tcttttcacc attgataacc caagtatcgc cctggcgttc agcgactgtt tcaagacctc
     6421 ccgcaacgtc cgaaccgtgt tctggttcag ttaaagcaaa gcatgtacgc agttcatgtg
     6481 actgtaattt aggtacatat ttcgcaattt gttctttgct acctccgaaa tagaaagtgt
     6541 tatgccctaa accttggtga acaccgagta gggtagctaa ggaaatatca aatcgcgcga
     6601 gtaggtaaga catgaaaaac tgaaatagtt gactaggcat tttggcgttt ggacgatcct
     6661 tgtaaagtaa tggattgtta aaataattta attctcccag atctttaaaa tagtcctcgg
     6721 gtacagtagc gtctatccaa tgttgattaa tattttcacg gtacttactt tctagcaatg
     6781 aatctacttg ttgtaaaaat tcgacttcac cgtctgttaa acctttagca atactaagta
     6841 catcttcagg aaataatgtt tttaagaccg tttctttttc aaatgtcata taaattcctc
     6901 ctaaaaataa tatgaatact aatgtgaaat gcatttaatt caaaaacaac acgctttatt
     6961 tgtaaacgct tacactaaat gtcaaaaatt tttatcacct ttaaagtgtt tgtgagactt
     7021 tgtcattcat catttgtcga atcgcaagtt tatctggttt ctgcgtactg tttaacggca
     7081 tatgtgtcac tggtacatac attcttggga ctttataacc tgctaaacga cttcgcatat
     7141 gttgatctaa aatttcagcg taatgaggtt catcttcgcg aagtataatg gctgcagcaa
     7201 ttgattcacc atattttgga tgatcatagc caacgaccac acaccggtct actagtggat
     7261 gctcagctaa agcattttcg acttcggatg gtaagacatt ttcgccacca gttatgatta
     7321 attctttttt gcggtcaata ataaatatat cgccatcgtc gtccatcttc gctaagtcac
     7381 cagttaataa ataacgacca tgaaatgctt tggcagtctc tgctggttta ttccaatatc
     7441 ctggcgtgac atttttagcc ttaattgcaa gttcgccaat ctcaccagta ggtacttcct
     7501 caccgttatc atcaaggata cgtgcatcaa cgaacatgac tgctttacca atactcattg
     7561 gcttacgttt tgaattttcc ggtgtattaa caagtacaag aggtgcttca gttaaaacat
     7621 agccgttaat aatgtttatg ccatattgtt taaaagctgc ttggatactt ggtaatggtt
     7681 gtgaaccacc ttggatgata taatccatag ctttaaaatt ttcaggatta aaattactag
     7741 cacgtagcgt actataatac attgtcggaa tcatgataat aaatgtaggg tgatattgtg
     7801 caatcatgtc attcaattct tcgccgttaa agtaacgttg aagaataagt gtgccacctg
     7861 acatcaatac tggtaataca gtatcgttaa accctaaaac atggaacatt ggtgttgata
     7921 caatcgtaat atagtttgaa ttgaacttat acgtcagctc taagtttgca ccgttatgaa
     7981 caaatgattc atatgagaac atcacacctt taggtgatcc ggttgtacca cttgtataaa
     8041 ttaatgctgc aagatcttgt ggttcgacag gtgttgcttg aaaaggttgg tgataatctg
     8101 gatttacgat ttcatcatat tgcgctacat caatatccat atgcaataag ttttggtcaa
     8161 tatcggtgag tgaacttaaa tgtttttcag catagaagag cagttttaat tgtgcatctt
     8221 ccacaatggc tgcaatttct tttgggttaa gccgccaatt caatggtaaa aaaaccgcac
     8281 ctgttttaaa acaagcaaac aataaatcta atattgcaat atcatttggc gcaaaaatac
     8341 cgataacatc gcctttttta acaccttgag atgttaaata atgtgccata ttatcagcgc
     8401 gtgcattgag ttgttggtat gtccaagatg tttgttttgc gtgatcaata acggcaggct
     8461 tgtcatcatc gaagtctgaa cgcgttttta tccaatcgaa attcattagt ataccccctt
     8521 tagcttcact ttcatacttt atgaattgat tgtttaagtt gtccccattt ttctttgtaa
     8581 atgctggtat caattaattt taaatgatca gcaataattg gtttaaaagc catttgattc
     8641 aaaatatctt tatgcaaatc aagacctggt gcaatttcaa ttagtttcaa gccttgattg
     8701 gtgagttcga atactgcacg ctcagtaaca aaatagactt cttgctcgag tgattgtgaa
     8761 tattgtgcat taaagtcgat atggctcaca tctgatacaa atttctggtt ttgtccttca
     8821 gtttcaatgt ttaatcgttg attatggcat gagacatgac tgccagctac aaaagtacct
     8881 gaaaagataa ttttatttac agattgcgta atgtctataa agccaccaca tccatttagt
     8941 cggtcattga agtaagacac gttgacattg ccgtattgat caacctcagc aaagctaaga
     9001 taggcaactg atacaccatt gttataaata aaatcccatg ctcgatcatg aggcatgcgc
     9061 acatctgcat tgtaattcat accaaaatgt tcacgactcc caacgaatcc accgaaaatg
     9121 ccaacatcta aaatcggttg cacatcatgt tcaacacatt cttcgtgcaa taaattagag
     9181 agttcattat tgatgccata accgatgcta attgtatcgc cataagttaa aaactgagca
     9241 gcacgtcgga gaatcaattt gcgactatta aaaggtaatg cgggttcagg tattccatca
     9301 attcgttctt ctccagacaa ggctggtaaa taatgactct gaattacttg gcggtgattc
     9361 ttttcatctt ctgtgacgta tacataatcg acaagatttc ctgggataac aacttcattc
     9421 ggttttagtt gatatccgtc aactaaagct ttaacttgta caataacttt cccatgattg
     9481 gctttcgcgt ttaatgcgac atgataacac tcgctcaagt acgcttcttg agttaaataa
     9541 atgttacctt gttgatctgc gtatgttcct ctcagtagtg ccacatcaac gctagggaat
     9601 gtgtaatgta agtatgtttc atcgttgatg gttactaatg aaactaaatc atcagttgtt
     9661 cgtgtattta ctttaccgcc accgtatcta ggatcaacag ctgtgtttaa tccgatttta
     9721 gtaataactc caggtaataa ttgattactc tgacgataat gagttgcaat gataccttgt
     9781 ggtaaaaaat aagcttcaat gtcattattt ttcattgctt gtgccgtttt ggaagaagcc
     9841 gttaaaatgc tcataatgac acgtttaatc atgcgacgtt ctataaaatc atctaaatcc
     9901 ggtgcggcac ctaaactatg gatatcattc gctaatataa acgttaaatc tttgggcgta
     9961 tgatatgtgt catgttgcgc taacacagca cgtagaactt cggcgggtaa gttggctaca
    10021 gctaatgctg gtaaaccaat cacatcacca tctttaatga tatgttgtaa gtcgtgccat
    10081 gtgatttgtt tcaagcaagt cacctccatc acatttgata aaatatagcg tttttacact
    10141 ttgtgtaaac ccttacaaga aatataacat aacgacgttt aaaatcaatt agaaatatct
    10201 ttttattctg ataatagaca cagtatagac acattttgat ggttgataac aattgtaata
    10261 tcaagggttt g
//
LOCUS       DBNENW010000092        10258 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNENW010000092 DBNENW010000000
VERSION     DBNENW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.092"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(221..643)
                     /locus_tag="AC1LW8_001813"
     CDS             complement(221..643)
                     /locus_tag="AC1LW8_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773640.1"
                     /translation="MKSVNILLNLLPTELKNMLENKDMENILTYFMSNEISDEKLVSY
                     LSNLANQINTIEYHEMVASIYHFHFNYIDNAYDLAYYHYWQSLEISQFNNANLLYEFL
                     EILGEPDFNMISHENIQLVANKILERDPNNKLAIKYIN"
     gene            complement(644..1228)
                     /locus_tag="AC1LW8_001814"
                     /pseudo
     CDS             complement(644..1228)
                     /locus_tag="AC1LW8_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198462579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAR2788 family putative toxin"
     gene            complement(1497..1649)
                     /locus_tag="AC1LW8_001815"
     CDS             complement(1497..1649)
                     /locus_tag="AC1LW8_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773641.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFHNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            2012..2419
                     /locus_tag="AC1LW8_001816"
     CDS             2012..2419
                     /locus_tag="AC1LW8_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HIP4773642.1"
                     /translation="MELSIFYNGQFFVALVEYKMENKSKFIQYTFGNEPDDIEVLDFI
                     HHQLMKMIDDVQTIVYTKNISRKVNPKKLQRQIAKEQKKPKYSTQAQIAIKKELELKK
                     KQKRKRYKEKRDAFQKRKREIKKVKAKEKHKGH"
     gene            2466..2948
                     /locus_tag="AC1LW8_001817"
     CDS             2466..2948
                     /locus_tag="AC1LW8_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HIP4773643.1"
                     /translation="MILISSCLIGDNVRYDGGNQLNVRLKKLIDSGKAIHACPELLGG
                     LLIPREPAEIIGGDGFDVWNDAAKVVTISNKDVTDDYKHGAIVTLKILKKYQCDTVIL
                     KANSPSCGSQEIYDGNFTGNKKKGVGVATALLINEGIKVYDENTFFDQNMNETIVHEK
                     "
     gene            3148..3837
                     /locus_tag="AC1LW8_001818"
     CDS             3148..3837
                     /locus_tag="AC1LW8_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIP4773644.1"
                     /translation="MEKVAILVDGGYYRKISAKVYGKVTAKERADELYSYCNRHLKET
                     HFKEEIYNKLYRIFYYDCPPIDKIVYHPLLKKNVNFSNTDTKKWTEDFFKEMSKKRKV
                     ALRLGELSEYSVEYNLKYSITKKLLNGSIDLNDLKEKDFSLSLQQKGVDMKIGLDIAS
                     LSFKHQVDKIILIAGDSDFVPAAKLARTEGIDFVLDSLGADIRNNLSLHIDGRRTCDE
                     KFKFYLNQLFK"
     gene            complement(4096..4455)
                     /locus_tag="AC1LW8_001819"
     CDS             complement(4096..4455)
                     /locus_tag="AC1LW8_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIP4773645.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGILLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(4474..5319)
                     /locus_tag="AC1LW8_001820"
     CDS             complement(4474..5319)
                     /locus_tag="AC1LW8_001820"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4773646.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNPESMVEVFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            5791..6471
                     /locus_tag="AC1LW8_001821"
     CDS             5791..6471
                     /locus_tag="AC1LW8_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HIP4773647.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGITKTNRGVYYDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDFKLRKQLISQSGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGRQI
                     QKILVEMK"
     gene            6757..7452
                     /locus_tag="AC1LW8_001822"
     CDS             6757..7452
                     /locus_tag="AC1LW8_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HIP4773648.1"
                     /translation="MKMKSIVKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            7743..8813
                     /locus_tag="AC1LW8_001823"
     CDS             7743..8813
                     /locus_tag="AC1LW8_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HIP4773649.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTQQTKMTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKL
                     QENRMADVIDGTNIDNIEVNIK"
     gene            9177..9881
                     /locus_tag="AC1LW8_001824"
     CDS             9177..9881
                     /locus_tag="AC1LW8_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HIP4773650.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKHQKFTRIQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT         3815 a         1423 c         1726 g         3294 t
ORIGIN      
        1 agtagtatta gtatgttgtg aattgttata gaatttgact tgttagcaat ttcgtagtta
       61 ttgtaatagc aagtactgta tggttttatt ttttaaaggt agtgaaataa tattggattt
      121 ttgaagtgaa atatttgtag atggatacaa cgaagcatat tagaaataca aaaggaacga
      181 tgaaattaaa tgtccatcat tccttatgcc tagtagtttt ttagttaata tatttaattg
      241 ctaacttatt attagggtct ctttcaagaa ttttatttgc gaccaattga atattctcat
      301 gtgaaatcat attaaagtct ggttctccaa ggatttctaa aaattcgtat aataaattag
      361 cattattaaa ttgcgaaatc tctaacgatt gccaataatg atagtatgct aaatcatagg
      421 cattatctat gtaattgaaa tgaaagtgat agatgcttgc gaccatttca tgatattcaa
      481 ttgtatttat ttgattcgca agatttgata aatatgatac taatttttca tctgaaattt
      541 cgttcgacat aaagtatgtt agaatattct ccatatcttt attctctaac atatttttta
      601 attcagttgg taataaattt aatagaatgt taacgctttt catttattta acccatccat
      661 tcgatatata tattgccatt ttttcgtgta taagttatga ctacagttct atcactcatg
      721 acaagtgttt tttggtatgc ggactttttg tatcggcttt gctttccata cctcataaca
      781 tgcgacatta ctttagaaac atctttccaa ttgtttttag taactttatt ccaattatgt
      841 ttactagcaa gaatatgttt tcttttatta gctcctagat tggctacaga aggtaaaaat
      901 ttaccaaaac tctttgtttt gatagcggtt tgagttgctt tttttccata tgttttaatt
      961 gcatagttta ttccgtatca gctactgcag ctaatattac taatggcaat gcagaagatt
     1021 tagcttcaat tgagttgaat tttattattt cgccagtttc tttgtcgatt aatgtagctt
     1081 catattcttc attttcaaat tgttctacga aaacgtcata atccttattt attagttttc
     1141 cattttcatc gacgtatctg cccttatcat ccatagtatt agtattcaaa tcatattgaa
     1201 tatttttatc taaatcaata tcttttttcg tttttccact aacgtctttt tcaagcttta
     1261 tatttacaat ttttaagcag aagtatccca ttatttcaag gtctgattta tctataatta
     1321 aatacgtacg tatggaattt tgatcgttca tttttattgc tttatgtttt aggaaatgtg
     1381 ctattgaagg gtttaaagat gtttggaatt tcttggtttc ttgacttata tagttatgaa
     1441 agagggcgtt gtcctttatt aaatttctga gatcagaaat gctataaata aagtgattaa
     1501 gattttttgt tttcatgtaa tttaagaaat ggatttgttt tttgtgggtt atttttaatt
     1561 ctattatgaa atggtttgaa ttttgcagtt gcaaatatga tagctgcttc ttttgacaaa
     1621 gtaatagaac ctttttcttc tggtaccatg atattcactc cttgtacaat tcaatttaag
     1681 tattataaaa taattttact atacaattac ctttgtgtaa atcgtttctt attgttacca
     1741 aattatttgt ttaaaagcat tatgcaatgc caatcaacac taatggggtg taagtatttc
     1801 cctgttaata ttttattagc gttatttaca tataccttct gtaatgtgaa tttttaaaat
     1861 tacagtttac atgtaacgtt gtgcaatata ttctgtatat agaagtgtaa aaacacaatt
     1921 gggttggtag tcccaatgca gttttaaaac tgtcagtaac cttcctcctc gggtcgtccg
     1981 tcatttctaa taaaactaga ggagggatag catggagcta tctatttttt acaatgggca
     2041 attttttgta gcattggtag aatataaaat ggaaaataaa tctaaattta tccaatacac
     2101 atttgggaat gaacctgatg atatagaggt attggatttt attcatcatc aattaatgaa
     2161 aatgattgat gatgtgcaaa ctattgttta tacgaaaaat atttctagaa aagtaaaccc
     2221 gaaaaaacta caacgacaaa ttgctaagga gcaaaagaaa cctaaatatt ctacccaagc
     2281 acaaatagct attaagaaag aattagaatt aaagaaaaag caaaagcgga agcgctataa
     2341 agaaaaacgt gatgcatttc aaaaaagaaa aagagaaatt aaaaaggtta aagcaaaaga
     2401 gaagcataag gggcattagc gatacgacaa ataaattaaa acaaatagat ttataggtgg
     2461 ttgggatgat tttaattagt tcgtgtttga taggcgataa tgtaagatac gatggtggta
     2521 atcaattgaa tgttagattg aaaaaattaa tagacagcgg aaaagctatt cacgcatgtc
     2581 cagaattact tggtggatta ttaattccta gagaacctgc agaaattatt ggtggcgatg
     2641 gttttgatgt atggaatgac gctgcaaagg ttgttactat ttcgaacaaa gacgtaactg
     2701 acgattataa acatggagca atagttacat taaaaatttt gaaaaaatat caatgtgata
     2761 cagttatttt aaaagcaaat agcccatcat gtggatcaca agagatatat gatgggaatt
     2821 ttacaggaaa taagaaaaag ggtgtaggtg tggcaactgc tttactcatt aatgaaggta
     2881 taaaagttta tgatgaaaat acgttttttg accaaaacat gaatgaaacg atagtacatg
     2941 aaaagtaagt tttgaggata tacgtaataa accaaacatt taagttgtaa gatactttat
     3001 aaaatatttt ttaaaatcgt tgaaatgata ttcaagttgt gataatatat cggtacagat
     3061 aattttgtta acagatccat gagagtagga accccaccat acgggaattt cgaaatcatg
     3121 gattttttga tataaaggag tgatgtaatg gaaaaagtag ccattttagt agatggtgga
     3181 tactatagaa agataagcgc aaaagtctac ggaaaagtta cagcgaaaga aagagctgac
     3241 gagttatata gttattgcaa tagacactta aaagagacac atttcaaaga agaaatatat
     3301 aataaattat acagaatatt ttactatgat tgtcctccta ttgataagat agtttatcac
     3361 cccttattaa aaaagaatgt aaatttttcc aatactgata cgaaaaagtg gacagaagac
     3421 tttttcaaag aaatgagcaa aaaaagaaag gtagcattga ggttaggaga gttgagtgag
     3481 tattcagttg aatacaactt aaaatactct attacaaaaa aattattaaa tggaagtatc
     3541 gatttaaatg atttgaaaga aaaagatttt tccctatcat tacaacaaaa aggtgttgat
     3601 atgaaaatag gtttagacat agcttcatta tcatttaaac atcaagtaga taaaattatt
     3661 ttaatagctg gtgatagcga ttttgtacct gctgctaaac tagcaagaac agaaggaatt
     3721 gattttgtat tagattcttt aggggcagat attagaaaca acttgtcatt acacattgat
     3781 ggtaggcgta cttgtgatga aaaatttaaa ttttatttaa atcaactatt taaataataa
     3841 tttttttaaa acgcatcttc tactttacag acgtttatag ctattagtat ttatcagcta
     3901 aaaattaaat cagatatggg ttttcgtcat taaataattt ttctataagg aaacagaggc
     3961 aacgttactg tcacctctgc ttgaaataat taggatgaaa tactctgagt ctaaagtcca
     4021 cacccaattc gctcacatcc tccaaacctc caaacctcca aacctccaaa cctccaaacc
     4081 cccaaacacc aatatttacc aaagcattga tatgagtgcc aatactggca atgtaccttg
     4141 tttgaaaaag atactaatat tgcttgaaag gccaccataa atagcaacgc caatgatata
     4201 cactaaaata gctgcgcata tttcttttgg attactgctg ataaacaaac cgtatattag
     4261 caaaattccg attaaaccgt tatatacgcc ttggttcttc aaaagtaggt taatattttt
     4321 gtctttcaat ttatcgacgc ttatattaaa tgtctcgcta gtctttttgg aagttgtagc
     4381 aatcgtttca aggtacataa tatagaaaaa ctctaatgcc acaaatatga ttaaaattgt
     4441 tgagatgata ttcactgtaa cgctccttta ttattaaata ttttcttgta aaaatgattg
     4501 cagtgtctgt ggttgatcat tgactaattg ttggaaatca ttggattctt ggtctaatag
     4561 tcctcttgct cctgcgtcgt acattgatgc caataatgca ccaaagcctt taggttcatc
     4621 gtacatttct gcaaatgtct ctaatgaaac gggctcatat ttaatttctg tgcctgatgc
     4681 ctcagataaa attgcagcaa gttctttcat atcataactg tagcctgata ataaatagcg
     4741 tttgccccaa gtatctggat ttttaataat agcaatgaca cctctagcaa tatcatttct
     4801 agtaatataa ttaatacgac catcaccagc tggataaatc agtttatgca tattcatcaa
     4861 ttctggtaaa tatggtttaa gtggatccat gtacattgcc attcttacat aggtatagtc
     4921 aataccactt gttgccaata gacgtgctgc ataaccaaaa taaggactca tatggaatgg
     4981 attattatgc tgatctgcgt aataacctat gaaaatgata tgagcaacgc cactttgctt
     5041 tgccgcatat actaaatttt ccacttctgg aatacgtttg aatgatggat ggataatact
     5101 tggaataaac acaacggtat ccattccttt aaatacctct accatgcttt cgggattaaa
     5161 ataatctaat tgtcgaacag gaacttttcc gcgccaatct tctggaactt tctcaacatt
     5221 tctaacacca atgtgaaaat gatctatgtg atttgcaatg gcttgatttg taatatgtgt
     5281 gcctaaatga cctgtagcac ctgttaacat aatattcatt cacttcatct cctaatcttt
     5341 atatacataa cataatactt attcgattgt tttcaaaaca tttgatttta taaaaaattg
     5401 taatctgtat ttattatcgc tgtgtatagt aaatacgtat atgttattaa tgttgaaaat
     5461 accgtaatga cgcgttttag ttgatgtgta tcactaatat ctttggaaat tttaatcagg
     5521 tactacgaca atatgatgtc tgttttgtgt ctgaaagttt tacagttttt aaaataaaaa
     5581 tggtataaag tgtgatttgt ataaaaaaga gtctcgacgg ataagaattg attaataaca
     5641 gttagcattt tattaattac cttaacaatg attcaagttt agttaaatga ggtttaattt
     5701 gaaaggggat agcgcctcaa tataatgtag gtagattgtt catattacgt aattgaaaaa
     5761 tcaaatttaa atagattggg gctaaaaatt atgaaattta aagcgatagc aaaagcaagt
     5821 ttagcattgg gaatgttagc aacaggtgta attacatcga atgtacaatc agtacaagcg
     5881 aaaacagaag ttaaacaaca aagtgaggct gatttaaaac tttattataa tggaccaagt
     5941 tttgaatata aaaaagtaac tggatatgga tttattgaag gtaaagatag atttattgat
     6001 tttatataca atggacaata taataaaata tctttagttg gttctgataa agataaatat
     6061 aatgaagaag ttaacccaga tatagatgtg tttgtcgtta gagaaggaaa cggtagacaa
     6121 gctgataatc attcgattgg tggcataaca aaaactaata gaggagtgta ttatgactat
     6181 atacacacac caatccttga aatcaagaaa ggtaaagaag aaccacaaag tagtctatac
     6241 caaatttata aagaagacat ctcactaaaa gaacttgatt ttaaattaag aaagcaatta
     6301 attagtcaaa gtggcttgta ttcaaatggt cttaaacaag gtcaaattac aattacaatg
     6361 aatgatggca caacacatac aatcgattta agtcaaaaac ttgaaaaaga acgtatgggc
     6421 gagtctatcg atggcagaca aatacaaaaa attctagtag aaatgaaata atactttcta
     6481 acaacaaagc gctatgttga atagtgcttg ttatggaaat atatggaagt taagcgacgt
     6541 actgttgctt agcttctttt tttgagggga aaagttacaa aactcacaca aacagtcgca
     6601 ccacgcatta tcttttgctt aaatagctta atcatatttt atgaatagtt aaaaacaggt
     6661 taatgtgaat atctgaatac agctcctata atatgggtgt atggttcaaa ttacgtaata
     6721 aaacaatcta attataatag attggagcat acagctatga aaatgaaatc aattgtaaaa
     6781 ataagtttgt tattaggaat attagcaaca ggtgtaaaca ctacaacgga aaaaccagtt
     6841 catgccgaaa agaaacctat tgtaataagt gaaaatagca aaaaattaaa agcttattat
     6901 actcaaccta gtattgaata taaaaatgtg acaggttata tcagtttcat tcaaccaagt
     6961 attaaattta tgaatatcat agatggtaat tctgttaata atattgcttt aattggcaaa
     7021 gataagcaac attatcatac gggtgtacat cgtaatctta atatatttta cgttaatgag
     7081 gataagagat ttgaaggtgc aaagtactcc attggcggta tcacgagtgc aaacgataaa
     7141 gctgtcgacc taatagcaga agcaagagtt attaaagcag atcatattgg tgaatatgat
     7201 tatgactttt tcccatttaa aatagataaa gaagcaatgt cattgaaaga gattgatttt
     7261 aaattaagaa aataccttat tgataattat ggtctttacg gtgaaatgag tacagggaaa
     7321 attaccgtca aaaagaaata ctacggaaag tatacatttg aattggataa aaagttacaa
     7381 gaagaccgga tgtccgatgt tatcaatgtc acagatattg atagaattga aatcaaagtt
     7441 agaaaagcat aacacacata cttgacgacg aaataatttg aaattgaaat agagaggtta
     7501 agtgacgatc aaacgttgct taacttcttt ttaatgctta aaaatcattt caaaggcaca
     7561 tagaaacgct atattaacct cataatcact cattattttt tgcttaaatt acttaataat
     7621 acttcaataa ttgttaaaag gggtttaatg tgattatctt agaatgccat ctataatgat
     7681 gttgtatgat tcaaattacg taaaaagaca atcgaatata atatagattg gagcatacaa
     7741 ttatgaaaat gagaacaatt gctaaaacca gtttagcact agggctttta acaacaggcg
     7801 caattacagt aacgacgcaa tcggtcaaag cagaaaaaat acaatcaact aaagttgaca
     7861 aagtaccaac gcttaaagca gagcgattag caatgataaa cataacagca ggtgcaaatt
     7921 cagcgacaac acaagcagct aacacaagac aagaacgcac gcctaaactc gaaaaggcac
     7981 caaatactaa tgaggaaaaa acctcagctt ccaaaataga aaaaatatca caacctaaac
     8041 aagaagagca gaaaacgctt aatatatcag caacgccagc gcctaaacaa gaacaatcac
     8101 aaacgacaac cgaatctaca acgcagcaaa ctaaaatgac aacacctcca tcaacaaaca
     8161 cgccacaacc aatgcaatct actaaatcag acacaccaca atctccaacc ataaaacaag
     8221 cacaaacaga tatgactcct aaatatgaag atttaagagc gtattacacg aaaccgagtt
     8281 ttgaatttga aaagcagttt ggatttttgc tcaaaccatg gacgacggtt aggtttatga
     8341 atgttattcc aaataggttc atctataaaa tagctttagt tggaaaagat gagaaaaaat
     8401 ataaagatgg accttacgat aatatcgatg tatttatcgt tttagaagac aataaatatc
     8461 aattgaaaaa atattctgtc ggtggcatca cgaagactaa tagtaaaaaa gttaatcaca
     8521 aagtagaatt aagcattact aaaaaagata atcaaggtat gatttcacgc gatgtttcag
     8581 aatacatgat tactaaggaa gagatttcct tgaaagaact tgattttaaa ttgagaaaac
     8641 aacttattga aaaaaataat ctgtacggta acgttggttc aggtaaaatt gttattaaaa
     8701 tgaaaaacgg tggaaagtac acgtttgaat tgcacaaaaa attacaagaa aatcgcatgg
     8761 cagatgtcat agatggcact aatattgata acattgaagt gaatataaaa taatcatgac
     8821 attctctaaa tagaagctgt catcggaaaa acaagaagtt aagtgacaac ggcctacatg
     8881 ttgcttagct tcttttgtta tgttcgatga tttgagaacc cgaattttcg atgggtccaa
     8941 atatgacgtg gaagagtcct gaatttatct gtaaatccct atctatcggg tgtggagcac
     9001 aacggaatca gttttattta acgaacatta tagattcctt aatttactta ataatgattc
     9061 aatgattatt aaacatggtt taatgtgaaa ggtcaaatac gccaattata ataaagctgt
     9121 atgattcaat agacgtaagc gaacaaatct aataattacg aatggagcat acaactatga
     9181 aaatggcagc aattgcgaaa gcaagtttag cattaggtat tttagcaaca ggaacaataa
     9241 cgtcattgca tcaaactgta aatgcgagtg aacatgaagc aaaatatgaa aatgtgacaa
     9301 aagatatctt tgacttaaga gattactata gtggcgcaag taaggaactt aaaaatgtta
     9361 ctggttatcg ttatagcaaa ggtggcaagc attaccttat ctttgataaa catcaaaagt
     9421 tcactagaat acaaattttt ggtaaagata tagaaagatt taaagcacgc aaaaatccgg
     9481 gattagacat atttgttgtt aaagaagcgg aaaaccgtaa tggcacagtg ttttcatatg
     9541 gtggtgtcac taagaaaaat caagacgctt attatgatta tataaacgca ccaagatttc
     9601 aaatcaagag agatgaaggt gacggtattg ctacgtacgg tagagtacac tacatttata
     9661 aagaagagat ttcacttaaa gaactcgact ttaaattgag acagtattta attcaaaatt
     9721 ttgatctgta taaaaagttt cctaaagata gtaagataaa agtgataatg aaagatggcg
     9781 gctattatac gtttgaactt aataaaaaat tacaaacaaa tcgcatgagt gacgtcattg
     9841 acggtagaaa tattgaaaaa atagaagcca acattagata atgcaatgaa atatggataa
     9901 tagtaaaata tggatagtat agaggagtta ggcaacataa gttgcttagc ttcttttttg
     9961 tgttggagag atgaaaaaga agcgtatcga tgaataataa aaacaccaat aaaacttgtg
    10021 ggaatagttg atacctatag tcgcgcgttg tccttttcgt gacatgaaac aatgtggaaa
    10081 acataattaa attgagggaa agtgtgaata gttaaaaaag ctgcgttaag tttaaaaaat
    10141 agattaacgc tgttaggatt ctattaatta gcttaacatt ggttcaaaaa tagttaaaaa
    10201 gaggttaatt catagcttag tattccgctt atataatgat agtagattgt tcgtatta
//
LOCUS       DBNENW010000093        10063 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNENW010000093 DBNENW010000000
VERSION     DBNENW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.093"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            252..602
                     /locus_tag="AC1LW8_001825"
     CDS             252..602
                     /locus_tag="AC1LW8_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAUGI family uracil-DNA glycosylase inhibitor"
                     /protein_id="HIP4773651.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            689..1000
                     /locus_tag="AC1LW8_001826"
     CDS             689..1000
                     /locus_tag="AC1LW8_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 family protein"
                     /protein_id="HIP4773652.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            1015..1521
                     /locus_tag="AC1LW8_001827"
     CDS             1015..1521
                     /locus_tag="AC1LW8_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HIP4773653.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            1570..3181
                     /locus_tag="AC1LW8_001828"
                     /pseudo
     CDS             1570..3181
                     /locus_tag="AC1LW8_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(3172..3414)
                     /locus_tag="AC1LW8_001829"
                     /pseudo
     CDS             complement(3172..3414)
                     /locus_tag="AC1LW8_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505894.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(3404..4390)
                     /locus_tag="AC1LW8_001830"
     CDS             complement(3404..4390)
                     /locus_tag="AC1LW8_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HIP4773654.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            4487..6496
                     /gene="mecA"
                     /locus_tag="AC1LW8_001831"
     CDS             4487..6496
                     /gene="mecA"
                     /locus_tag="AC1LW8_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HIP4773655.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(6542..6970)
                     /locus_tag="AC1LW8_001832"
     CDS             complement(6542..6970)
                     /locus_tag="AC1LW8_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIP4773656.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(7067..7810)
                     /locus_tag="AC1LW8_001833"
     CDS             complement(7067..7810)
                     /locus_tag="AC1LW8_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HIP4773657.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(8607..8996)
                     /locus_tag="AC1LW8_001834"
     CDS             complement(8607..8996)
                     /locus_tag="AC1LW8_001834"
                     /inference="COORDINATES: protein motif:HMM:NF020129.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIP4773658.1"
                     /translation="MQLCNRTVLWNRDVGNIYTGSLYLSLISLLQNHTFQPEEKVCLF
                     SYGSGAVEEIFSGSIVKGYDKALDKEKHLNMLESREQLSVEEYETFFNRFDNQEFDFE
                     RELTQDPYSKVYLYSIEDHIRTYKIEK"
     gene            9032..9706
                     /locus_tag="AC1LW8_001835"
     CDS             9032..9706
                     /locus_tag="AC1LW8_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HIP4773659.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     gene            complement(9738..9977)
                     /locus_tag="AC1LW8_001836"
     CDS             complement(9738..9977)
                     /locus_tag="AC1LW8_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773660.1"
                     /translation="MSDNLSLFIDYINDNIIYGSEIKREKLENLFNQFAIKNVEKNIV
                     YDELKSLDITIIESQDSYKNKLKRLFSVLLQSKKI"
BASE COUNT         3678 a         1655 c         1505 g         3225 t
ORIGIN      
        1 ataaataaat aatatatata aggaagcaga gacacaaccc tatcatcaat acttataaat
       61 aatgagttga ttgtttgtca aaataggcgg aatttcagga tttccttggc ataccaacgt
      121 ttgtagcggg tttttatatg gaattttctc ttattcccac cgggattcgt aataaatgac
      181 aaatcaaata tatattatct caatgatgta tatgaactac atcactatta cactatctag
      241 gaggcaataa tatgaaaacc atcactcaag aactcaaaca atatataacc cacctattcc
      301 aactatctaa taacgaagct tgggaatgtg aagcgctgga ggaagcagca gaaaatattc
      361 taccggaacg ttttattaat gattctccac tggtacatct cacactcgag acttatacct
      421 actataatga tgaactacat gagctaagta tctatccatt tctaatgtat gccaataacc
      481 aactcatcag tgtcggatac ttagaccatt ttgacatgga ctttctgtac cttacagata
      541 ctcaaaatat cattattgat gaacgtcatt tactaaaaca aggaggtcag gaccatgaat
      601 aattggatca tagttgctca attatccgtc acagttatca acgaaatcat cgacatcatg
      661 aaagaaaagc agaagggagg gaaatagcat gaaaatcaat cgatacatca caagaggaat
      721 taatgaaagt attccactag accttcaaat cttactttgg cacatggtag aaaaaaaaga
      781 taaccagcct catacagatt acctacacat ttttaaatta caagaagatg agaatatact
      841 ctcaatcaca catgaacaag aacaacccac atacaaattg gaatatcact atacaaacta
      901 tgaaaaaaat caaaatgcat tacctaagaa agtctacgtc attcgtgaag atgacgtgga
      961 cgctttttat tatgtgatgc ttttaccaga agaatactaa aggaagtgac gatgatgaat
     1021 acaatcaaaa gtacgataca cacagaagcc atatttagcg atgatgaaca gcaccgctat
     1081 ttactcaaga aaacatggga tgaaaagaaa gctgcatgca cagtgataac gatgtatcct
     1141 catttagacg gtgtattatc actcgatctc acaactgttc ttatcctcaa ccaattagcc
     1201 aattcagaac gatacggtgc tgtatatctt gtaaatctat tctctaatat tagaacaccc
     1261 gaaaacctca aacatatcaa aaatccatac gatgagcaca ctgatattca tttgatgaaa
     1321 gcgattagtg aaagtgacac agtgattctt gcttatggtg cctatgcgaa gcgaccagtt
     1381 gttatcgacc gtgtcgaaca agtgatggaa atgttaaaac ctcataaaaa gaaagtaaaa
     1441 aagctcatca atccagtaac aaatgaaatt atgcatccac tcaaccctaa ggcacgtcaa
     1501 aaatggattt tgaaatcata gcaaatacat ggaggtcaat atggaaacaa aacaaatcaa
     1561 catcgtttta ctgtaaatga tattgttgaa acaattcctg acaatgaatt cgacgaattc
     1621 agacatcacc gtggtgcaac ttcgtaccat cctaaaatga tgttaaaagt gattctatat
     1681 gcctacacac aatctgtatt ctcaggtcgt aaaatagaaa aaatgcttaa tgatagcatc
     1741 cgaatgatgt ggctatcaca aaatcaaaaa ccttcttata aaacaattaa tcaatttaga
     1801 gtaaatccaa aagtagatgc tttattagaa tctttattta ttcaatttta cagtcagtgt
     1861 gtaaaacaaa atcttataga tgataaagct atttttattg atggtacaaa aattgaagca
     1921 aatgccaatc gatatacatt tgtatggaaa aagagtattc aaaaccatga atcaaagatg
     1981 aatgaggatt ctaaagccct ctaccatgaa ttggtaacca ataaaatcat accggaaatt
     2041 aaagaagatc atgataatga attaacaaaa gaagaaatag atttgattgg tagtcactta
     2101 gataaagaaa tcgaagattt aaaccaacat atcaacaatg aaaaatgtac taaaacaaga
     2161 aaacaaatac gtctcaaaag aactaaaatc aaaaaataca aaaagcaaat caatgattat
     2221 tttgagcgaa agtatcgata cgaatttcaa aaatctattt taaaggatag aaatagttat
     2281 tctaagacag attatgatgc gacatttatg agaatgaaag aagatcacat gaaaaatgga
     2341 caacttaagc cagggtataa tttacaaata gcaacaaatt cccaatttgt tttatcttat
     2401 aatgtgtatc aaaatccaac ggatactaga acgatgattc catttttaaa ttcaattcaa
     2461 gagacctacg gtcatttacc tgaatatatt gtagctgatg caggttatgg tagtgaatca
     2521 aattataagg caattataga tgactttaat cgaacgccac tcataacata tggaatgttt
     2581 ataaaagata aaactaaaaa atataaaagt gacatcttta atactcaaaa ttggaactat
     2641 gacgaaatta atgacgaatt catttgtccg aataataaac ggctaggttt taaaagatat
     2701 gcctatcgtc atgataagta tggttataag cgagacttca aattatatga atgtgatgat
     2761 tgttcagaat gtcctctgaa aaatcaatgt atgaacttca attcaaaaac aaacaaaaaa
     2821 aataatgaag aattataact gggaatattt taaatcccaa attaacaaaa agctttcaga
     2881 accagaaaca aaaaatatct acagtcaaag aaaaattgat gtggaacctg tttttggatt
     2941 tatgaaggct attttgggtt tcactcggat gtctgtccga ggactcaata aagtcaaaag
     3001 agaacttggt tttgtattaa tggcacttaa tataagaaaa gtagtagctc aacgagctga
     3061 aaataatcaa aaaatttata aaaaagacaa tttctatatt atttcaatag aaattgtctt
     3121 ttttcactta tccaagaact ttatgtcccg gactcattat gatttatttt tttattgctg
     3181 acttgccatt aattctgctt gctctacaac ttgttccaca gccatttttt gaagatctgg
     3241 tggatagcca tatttcttta ataggcgtct aacttgaact ctcattttag ctttagcact
     3301 gtctcgttta gaccaatcaa cactcatgtt atccttaact gttttagtta actcatgtgc
     3361 aatggctctt aactctttat cgcccaaagc ttctttagct gtttcatgtg aagctcgata
     3421 tactacatac aatatacgca acaatacgct tgtttcgatt aggtttaggc attgaatcat
     3481 aaagtgcaat atacttaaca cgttctttaa tatttgaatt aaaacctagt aaatattgtg
     3541 ctgccacatt atttatgtgc tgagatttta aaatagagca ttttaatatc gattcaccat
     3601 aacgtatatg ttcatggcga ttcaaaattt ttaaaacgtt tctatcacat actttttcac
     3661 agtcattgtc catcattgtt ttacttatat atagtgcagg attaaaccag aatatcattt
     3721 taaaaacaac ataaagctgg ttgaatatta agtcatgact tttcacatgt gatagttcat
     3781 gtagaataat atattcaatt tctttgtcat tcatggtttc gactacgaca gttggtagta
     3841 caatttggga tttcactaaa ccaaatacca tcggattatc aatgtttgaa ctataactaa
     3901 ttgttatatg ctttttgtag aactgcatct tactttgaca tactttaagt cgttcattaa
     3961 gatatgacga ttccaatgac gaacttttaa taacatcaat ttgtcggaat gccttaatca
     4021 tataaaataa gcacaacaaa ctaccaaata cccatatcaa aagaatcata tacgttatat
     4081 ttgaggtctc aaactgatta acattaattg ctaagtcttt cgtaacagat gattgttgac
     4141 catctaacat atgactaacc gaagaagtcg tgtcagatac atttcgattc atcatatctt
     4201 ttgaaaatgt aaaattcgat attttgtaaa atggtattaa tggaattaac gtggagacga
     4261 gcactaataa ccaaatctta tgtgacataa tattttgagt atattttata tagagcattc
     4321 tcactaaaaa aattacacat atcgtgagca atgaactgat tatacttaac attaaaaaag
     4381 atgataacac cttctacacc tccatatcac aaaaattata acattatttt gacataaata
     4441 ctacatttgt aatatactac aaatgtagtc ttatataagg agtatattga tgaaaaagat
     4501 aaaaattgtt ccacttattt taatagttgt agttgtcggg tttggtatat atttttatgc
     4561 ttcaaaagat aaagaaatta ataatactat tgatgcaatt gaagataaaa atttcaaaca
     4621 agtttataaa gatagcagtt atatttctaa aagcgataat ggtgaagtag aaatgactga
     4681 acgtccgata aaaatatata atagtttagg cgttaaagat ataaacattc aggatcgtaa
     4741 aataaaaaaa gtatctaaaa ataaaaaacg agtagatgct caatataaaa ttaaaacaaa
     4801 ctacggtaac attgatcgca acgttcaatt taattttgtt aaagaagatg gtatgtggaa
     4861 gttagattgg gatcatagcg tcattattcc aggaatgcag aaagaccaaa gcatacatat
     4921 tgaaaattta aaatcagaac gtggtaaaat tttagaccga aacaatgtgg aattggccaa
     4981 tacaggaaca gcatatgaga taggcatcgt tccaaagaat gtatctaaaa aagattataa
     5041 agcaatcgct aaagaactaa gtatttctga agactatatc aaacaacaaa tggatcaaaa
     5101 ttgggtacaa gatgatacct tcgttccact taaaaccgtt aaaaaaatgg atgaatattt
     5161 aagtgatttc gcaaaaaaat ttcatcttac aactaatgaa acagaaagtc gtaactatcc
     5221 tctaggaaaa gcgacttcac atctattagg ttatgttggt cccattaact ctgaagaatt
     5281 aaaacaaaaa gaatataaag gctataaaga tgatgcagtt attggtaaaa agggactcga
     5341 aaaactttac gataaaaagc tccaacatga agatggctat cgtgtcacaa tcgttgacga
     5401 taatagcaat acaatcgcac atacattaat agagaaaaag aaaaaagatg gcaaagatat
     5461 tcaactaact attgatgcta aagttcaaaa gagtatttat aacaacatga aaaatgatta
     5521 tggctcaggt actgctatcc accctcaaac aggtgaatta ttagcacttg taagcacacc
     5581 ttcatatgac gtctatccat ttatgtatgg catgagtaac gaagaatata ataaattaac
     5641 cgaagataaa aaagaacctc tgctcaacaa gttccagatt acaacttcac caggttcaac
     5701 tcaaaaaata ttaacagcaa tgattgggtt aaataacaaa acattagacg ataaaacaag
     5761 ttataaaatc gatggtaaag gttggcaaaa agataaatct tggggtggtt acaacgttac
     5821 aagatatgaa gtggtaaatg gtaatatcga cttaaaacaa gcaatagaat catcagataa
     5881 cattttcttt gctagagtag cactcgaatt aggcagtaag aaatttgaaa aaggcatgaa
     5941 aaaactaggt gttggtgaag atataccaag tgattatcca ttttataatg ctcaaatttc
     6001 aaacaaaaat ttagataatg aaatattatt agctgattca ggttacggac aaggtgaaat
     6061 actgattaac ccagtacaga tcctttcaat ctatagcgca ttagaaaata atggcaatat
     6121 taacgcacct cacttattaa aagacacgaa aaacaaagtt tggaagaaaa atattatttc
     6181 caaagaaaat atcaatctat taactgatgg tatgcaacaa gtcgtaaata aaacacataa
     6241 agaagatatt tatagatctt atgcaaactt aattggcaaa tccggtactg cagaactcaa
     6301 aatgaaacaa ggagaaactg gcagacaaat tgggtggttt atatcatatg ataaagataa
     6361 tccaaacatg atgatggcta ttaatgttaa agatgtacaa gataaaggaa tggctagcta
     6421 caatgccaaa atctcaggta aagtgtatga tgagctatat gagaacggta ataaaaaata
     6481 cgatatagat gaataacaaa acagtgaagc aatccgtaac gatggttgct tcactgtttt
     6541 attatgaatt attaataagt gctgttactt ctcccttaaa tacaatttct tcattttcat
     6601 tgtatgttga aagtgacact gtaacgagtc cattttcttt ttttatggat ttcttatttg
     6661 taatttcagc gataacgtac aatgtattac ctgggtatac aggtttaata aatttaacgt
     6721 tattcatttg tgttcctgct acaacttctt ctccgtattt accttcttct acccataatt
     6781 taaatgatat tgaaagtgta tgcatgccag atgcaatgat acctttaaat ctactttgtt
     6841 ctgctttttc tttatctata tgcatatatt gaggatcaaa agttgttgca aattggataa
     6901 tttcttcttc tgtaatatga aggctttttg ttttgaatgt ttctcctact ataaaatcat
     6961 cgtatttcat atatgtctct ctttcttatt caaattaatt ttttagtatg taacatgtta
     7021 aaggtaagtc taccgtcact gaaacgtaag actcacctct aactttctat tgagacaaat
     7081 gcaccatttt atctgcattg tctgtaaaga taccatcaac tccccaatta gcaagttggt
     7141 ttgcacgtgc tggtttgttt acagtccata cgttcaattc ataacccgct tcttttacca
     7201 tttttacttt tgctttagta agtttggcat cttcagtgtt tactatttta gcattacagt
     7261 aatctaaaag tgttctccag tcttcacgaa acgaagttgt atggaatata actgctctgt
     7321 tatattgtgg catgatttct tctgcaagtt taacaagcac aacattaaag cttgaaatga
     7381 gcacttcttg attctgattt aagtttgtta attgttcttc cacttgctta accatacttt
     7441 tagaaagtgc tagtccattc ggtccagtaa taccttttaa ttctacattt aaattcatat
     7501 tatattcatt tgctattttt actacatcat cgaaagttgg caaatgttca tctttgaatt
     7561 tttcaccaaa ccaagatcct gcagaagcat ctttaatttc atcataattc aattcagtta
     7621 tttccccgga catatttgta gtccgttcta aataatcatc atgaatgata atcagttgtt
     7681 catcttttgt aattgcaaca tctaactcca accagtttat accttctact tctgaagcag
     7741 ctttaaatga tgcaattgta ttttccggag ctttactagg taatcctcta tgtccatata
     7801 cagttagcat attacctctc cttgcatttt tattttttta attaacgtaa ctgtattatc
     7861 acattaatcg cacttttatt tccattaaaa agagatgaat atcataaata aagaagtcga
     7921 tagattcgta ttgattatgg agttaatcta cgtctcatct catttttaaa aaatcattta
     7981 tgtcccaagc tccattttgt aatcaagtct agtttttctg taccccttat ctgcaatttt
     8041 acttaggatt gcttttaact taccccttat cagcaatttt actgagaact gcttttaacg
     8101 cacctcttat ctgcaatttt gcctagaact gcttttaacg tacctcttat ctgcaatttt
     8161 actgagaact gcttttaact taccccttat cagcaatttt gcatggaatt gcttttaacg
     8221 tacctcttat ctgcaatttt acttagaact gcttttaaca aacctcttat ctgcaatttt
     8281 acttagaact gcttttaaca aacctcttat ctgcaatttt acttagaatt gcttttacta
     8341 ttcctcttat tagtataatc tcagtaagaa tgcgtataaa aatgaaaatt acaaccgatt
     8401 ttgtaagtgc tgacgcctga gggaatagta tgtgcgagag actaatggct cgagccatac
     8461 ccctaggcaa gcatgcacgt acaaaatcgt aagataaaaa aataagcata tcactgtaaa
     8521 ctttaaaaaa tcagtttagt gatatgctta tttatttcga gttaggattt atgtcccaag
     8581 ctcatcaagc acaatcggcc actagtttat ttctctatct tatatgttct gatatggtct
     8641 tctatactgt ataagtatac ttttgaatat ggatcttgtg tcaattcacg ttcgaaatca
     8701 aattcttgat tatcaaatct gttaaagaat gtttcgtatt cttcgactga taattgctct
     8761 ctagattcta gcatatttaa gtgtttctct ttatctaatg ctttgtcata tcctttaacg
     8821 attgaaccac taaagatttc ttctactgct cctgaaccat aactaaatag acatactttc
     8881 tcttctggtt ggaatgtgtg gttctgtaat aacgaaatta aacttaagta taatgatcct
     8941 gtataaatgt taccaacatc tctattccat aatacggttc tgttgcaaag ttgaatttat
     9001 agtataattt taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa
     9061 caaggatgtt atcactgtag ccgttggcta ctatctaaga tatacattga gttatcgtga
     9121 tatatctgaa atattaaggg aacgtggtgt aaacgttcat cattcaacgg tctaccgttg
     9181 ggttcaagaa tatgccccaa ttttgtatca aatttggaag aaaaagcata aaaaagctta
     9241 ttacaaatgg cgtattgatg agacgtacat caaaataaaa ggaaaatgga gctatttata
     9301 tcgtgccatt gatgcagagg gacatacatt agatatttgg ttgcgtaagc aacgagataa
     9361 tcattcagca tatgcgttta tcaaacgtct cattaaacaa tttggtaaac ctcaaaaggt
     9421 aattacagat caggcacctt caacgaaggt agcaatggct aaagtaatta aagcttttaa
     9481 acttaaacct gactgtcatt gtacatcgaa atatctgaat aacctcattg agcaagatca
     9541 ccgtcatatt aaagtaagaa agacaaggta tcaaagtatc aatacagcaa agaatacttt
     9601 aaaaggtatt gaatgtattt acgctctata taaaaagaac cgcaggtctc ttcagatcta
     9661 cggattttcg ccatgccacg aaattagcat catgctagca agttaagcga acactgacat
     9721 gataaattag tggttagcta tattttttta ctttgcaaca gaaccgaaaa taatctcttc
     9781 aatttatttt tatatgaatc ctgtgactca atgattgtaa tatctaaaga tttcagttca
     9841 tcatagacaa tgttcttttc aacatttttt atagcaaatt gattaaataa attctctaat
     9901 ttctcccgtt tgatttcact accatagatt atattatcat tgatatagtc aatgaataat
     9961 gacaaattat cactcataac agtcccaacc cctttatttt gatagactaa ttatcttcat
    10021 cattgtaaaa caaattacac cctttaaatt taactcaact taa
//
LOCUS       DBNENW010000094         9924 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNENW010000094 DBNENW010000000
VERSION     DBNENW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.095"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            198..962
                     /locus_tag="AC1LW8_001837"
     CDS             198..962
                     /locus_tag="AC1LW8_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001804768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin OB-fold domain-containing protein"
                     /protein_id="HIP4773661.1"
                     /translation="MNNCFGEFEVKYLTGFILILLLEEILTNSASAIEYSDLHHKSKF
                     DSKRLSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSK
                     KFINKDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQA
                     EIELTAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEQNIEYN
                     FYNLNGEYGYEVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1164..1433)
                     /locus_tag="AC1LW8_001838"
     CDS             complement(1164..1433)
                     /locus_tag="AC1LW8_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773662.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1747..2433
                     /gene="mtnN"
                     /locus_tag="AC1LW8_001839"
     CDS             1747..2433
                     /gene="mtnN"
                     /locus_tag="AC1LW8_001839"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIP4773663.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINNFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2453..2980
                     /locus_tag="AC1LW8_001840"
     CDS             2453..2980
                     /locus_tag="AC1LW8_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIP4773664.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            2981..4081
                     /gene="yqeH"
                     /locus_tag="AC1LW8_001841"
     CDS             2981..4081
                     /gene="yqeH"
                     /locus_tag="AC1LW8_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HIP4773665.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4095..4901
                     /gene="aroE"
                     /locus_tag="AC1LW8_001842"
     CDS             4095..4901
                     /gene="aroE"
                     /locus_tag="AC1LW8_001842"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIP4773666.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDDKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4905..5195
                     /gene="yhbY"
                     /locus_tag="AC1LW8_001843"
     CDS             4905..5195
                     /gene="yhbY"
                     /locus_tag="AC1LW8_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIP4773667.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            5198..5767
                     /gene="nadD"
                     /locus_tag="AC1LW8_001844"
     CDS             5198..5767
                     /gene="nadD"
                     /locus_tag="AC1LW8_001844"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIP4773668.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5757..6341
                     /gene="yqeK"
                     /locus_tag="AC1LW8_001845"
     CDS             5757..6341
                     /gene="yqeK"
                     /locus_tag="AC1LW8_001845"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIP4773669.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6342..6695
                     /gene="rsfS"
                     /locus_tag="AC1LW8_001846"
     CDS             6342..6695
                     /gene="rsfS"
                     /locus_tag="AC1LW8_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIP4773670.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            6698..7414
                     /locus_tag="AC1LW8_001847"
     CDS             6698..7414
                     /locus_tag="AC1LW8_001847"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIP4773671.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            7454..8140
                     /locus_tag="AC1LW8_001848"
     CDS             7454..8140
                     /locus_tag="AC1LW8_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIP4773672.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTI"
     gene            8232..8693
                     /locus_tag="AC1LW8_001849"
     CDS             8232..8693
                     /locus_tag="AC1LW8_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HIP4773673.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            8698..>9924
                     /locus_tag="AC1LW8_001850"
     CDS             8698..>9924
                     /locus_tag="AC1LW8_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIP4773674.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTSLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYKPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTR"
BASE COUNT         3619 a         1261 c         1807 g         3237 t
ORIGIN      
        1 gtcccacccc aacttgcaca ttatcgtaag ctgactttcc gccagcttct atgttggggc
       61 ccctgacttg aattgaaaaa aacttgttac aagcgcattt tcgttcagtc aactactgcc
      121 aatataactt cgtagagcat agaacattga tttatttccc agcctgttta tcatttatgt
      181 ctcgctctca aatgtaaatt aataattgtt ttggggagtt tgaagttaaa tatttaacag
      241 gatttatttt aatattattg ttagaagaaa ttttgacaaa ttcagcaagt gcaatcgaat
      301 attcagactt acatcataaa agtaagtttg attcaaagcg tctaagtaat gctaagatgt
      361 cattcatcaa tccaactcag cttgaaaata aaaacacaaa cgatagactg ttgaagcatg
      421 atttgttatt tcatgacatg ttcgtaaatg atgattggaa aaaagatttt aaagttgaat
      481 tcgaaaatga ggcactttca aagaaattta taaataagga tatcgatata tttgctggaa
      541 attatggata tggatgtcat gggggagcaa ccaataaaac gcaatgtagt tatggtggtg
      601 ttactttaag tgacaataat aagtacgatg attataagaa tataccttgt aatttatgga
      661 ttgacggaca tcaagcagaa atagaattaa cagcagtaaa aacgaaaaag aaaattgtta
      721 ccattcaaga attagatgtt caattaagaa attatttgaa tgagaagtat aagttgtacg
      781 aacaaggtgg cgacattgtt aaagggtatg ttaaatatca taatgatgat gaacaaaata
      841 tagaatataa tttttataat ttaaatggtg agtatggtta tgaggtatta aaaatgtatg
      901 ctgataataa aactatcaat agtgacaaat tgcatttaga tatctattta ttcaaatcat
      961 aatttaatta agttaatcat taataatctt tgacagtgaa atataaagta cctgtcatta
     1021 tactgttaca aataaaaaca gcagtaagga tgataacact tagaaaagtc ttactgctgt
     1081 tttctattag aattatcttt aatacacaca ggaattcgat acgaaacaaa tatgattaac
     1141 tgtgtaatta caaaataaaa ctactatagt aaaaagaaga taaagacgat gataataaat
     1201 gcatcgaata taattttctt gatgttttta tcaacaacat caggtaagga cataagtata
     1261 aatcctagaa ttagagtgta gcgacatatg ttcataattg ttgctgtaaa ctcgatatta
     1321 aatgaaaact ttaaaaatat acttataaat aaaatgacaa acgacgcaat tgcaataaaa
     1381 ctgccgtatg tagctatctt tgttacgaga ctattttgat tattttcagc cattgtaaaa
     1441 tcctcttatt tcatcattat tattttaaac atatcatttt cctgttattt aataaagttt
     1501 cttgacacga ttggttgatg taattgatat gattagtttt tgaaaaatag acaaattaga
     1561 agcatcgaat tcactgttat gagttcaaaa ataattattt atgataccaa gttattgaaa
     1621 gagcttttta taaaaataaa gcaaaagttt tatacagtaa tgaaaatcat gatacttgtc
     1681 gttgtagtga ttaaaatgtg ataaaataac aaatgctagg attttttaaa agaggtgaat
     1741 caaaggatga ttggtataat tggtgccatg gaagaagaag taacaatatt aaaaaataaa
     1801 ttaacacaat taagcgaaat ttcagttgca catgttaaat tttatactgg cattttaaaa
     1861 gatagagaag tagtgattac ccaaagcggc attggaaaag ttaatgctgc aatttctacg
     1921 acattattaa ttaataactt taaaccggac gtcattatta atacaggttc tgctggagct
     1981 ttagatgaaa gtttaaatgt aggtgacgtt cttataagtg atgatgtaaa atatcatgat
     2041 gcggatgcaa cagcatttgg ttatgaatat ggacaaatac cacagatgcc ggtagcattt
     2101 caatcaagta aacctttaat agaaaaagta tctcaagttg tacaacaaca acaattaaca
     2161 gctaaagtag gcttaattgt aagtggtgat agctttatcg gtagtgttga acaacgtcaa
     2221 aaaattaaaa aagcatttcc aaatgcgatg gcggttgaaa tggaagcaac tgcaattgca
     2281 caaacatgtt atcaatttaa tgtaccattt gttgtagttc gtgcagtttc agacttagca
     2341 aatggagaag cggaaatgag cttcgaagca tttttagaaa aagcagctgt atcatcaagt
     2401 caaactgttg aagcattagt gtctcaatta taaaaaggta aaggtgagtt aaatgggttt
     2461 agttcgcaag ttttttatgc cgaattcata tgttcaatca atatttcaaa ttgatttaga
     2521 caagttagtg gacaaaggcg ttaaaggtat tattacagat ttagataata cgctagtagg
     2581 ttgggatgtt aaagaaccta cagaacgtgt taaagcatgg tttaaggaag ctaatgaaaa
     2641 aggaatcact attacaatcg tgtctaataa taatgagtct cgtgttgcta gttttagtca
     2701 gcatttagac atcgatttta tttttaaagc gagaaagcca atggggaaag cgtttgataa
     2761 agcaataact aagatgaata tcagaccaga tcaaactgtt gttataggtg accaaatgct
     2821 tactgatgta tttggtggta atcgtcgagg tctatataca attatggttg ttccagttaa
     2881 acgaactgat ggctttatta ctaagtttaa tagattaatt gaaagacgat tattacgtca
     2941 tttcagtaaa aaaggttata tcacatggga ggaaaattga ttgtctgaca ttttaaaatg
     3001 tatcggttgt ggtgcgccac ttcaatctga agataaaaat aaacctggtt ttgtaccaga
     3061 gcataatatg tttcgtgatg acgtgatttg cagacgttgt ttccgcttga aaaattataa
     3121 cgaagttcaa gatgtaggat tagaaagtga agacttttta aaattattat caggacttgc
     3181 ggataaaaag ggtattgtcg tcaatgtcgt ggatgtattt gactttgaag gatcatttat
     3241 taatgcagtt aaacgtattg tcggaaacaa aaaaatcatt ttagcagcaa ataaattgga
     3301 tttattgccg aaacaaatta ataaacgtcg agttaaagaa tggttaaaac gaacagcaag
     3361 aaaatatggt ttggaagctg acgatgtcgt attaatttca gctgaaaaag gctggggcat
     3421 agacgactta ttatcatcaa ttgcgaatat tcgagaaaat gaagatgtgt atattgtagg
     3481 gacaacgaat gttgggaaat ctacattgat taataaactg attgaagcta gtgttggtga
     3541 aaaagatgta gtaacaactt caagattccc tggaacaact ttagatatga tagatattcc
     3601 tttagatgaa acatcattta tgtatgatac accaggtatt attcaagatc accaaatgac
     3661 gcatttagtt agtgaaaaag aattgaaaat tattatgcct aagaaagaaa taaaacaacg
     3721 cgtatatcaa ttaaatgagg cgcaaacatt attcttcggc ggtctagcgc gcatagatta
     3781 tgtatcaggt ggtaaacgtc cgttagtttg tttcttttct aatgacttga atatacatcg
     3841 tactaaaacg gagaaggcca atgatttatg gcgtaatcaa cttggcgatt tattaacgcc
     3901 acctggaaat ccacaaaatt ttgatcttaa tgaggtaaag gctgttagac ttgaaacagg
     3961 caaagagaaa cgcgatgtta tgatctctgg tctaggcttt ataactatag gaccaggagc
     4021 taaagtaatc gttcgtgttc ctaaaaatgt tgatgttgta ttaagaaatt ctattttata
     4081 aggtgattta aaaaatgaaa tttgcagtta tcggaaatcc tatttcacat tccttgtcgc
     4141 ccgttatgca tagagcaaat tttaattctt taggattaga tgatacttat gaagctttaa
     4201 atattccaat tgaagatttt catttaatta aagaaattat ttcgaaaaaa gaattagatg
     4261 gctttaatat cacaattcct cataaagaac gtatcatacc gtatttagat catgttgatg
     4321 aacaagcgat taatgcaggt gcagttaaca ctgttttgat aaaagatgac aagtggatag
     4381 ggtataatac agatggtatt ggttatgtta aaggattgca cagcgtttat ccagatttag
     4441 aaaatgcata cattttaatt ttgggcgcag gtggtgcaag taaaggtatt gcttatgaat
     4501 tagcaaaatt tgtaaagccc aaattaactg ttgcgaatag aacgatggct cgttttgaat
     4561 cttggaattt aaatataaac caaatttcat tagcagatgc tgaaaagtat ttagctgaat
     4621 tcgatatcgt tattaataca acaccagcgg gtatggctgg aaataacgaa agtattatta
     4681 atttaaagca tctttctccc aatactttaa tgagtgatat tgtttatata ccgtataaaa
     4741 cacctatttt agaggaagca gagcgcaagg gaaaccatat ttataatggc ttagatatgt
     4801 ttgtttacca aggtgcggaa agctttaaaa tttggactaa taaagatgct gatattaatt
     4861 ctatgaaaac agcagtttta caacaattaa aaggagaata acatatgctt actggcaaac
     4921 aaaaaagata cttaagaagt ttagcacaca atattgatcc gatttttcaa attggaaaag
     4981 gcggtatcaa cgaaaatatg attaaacaaa tagatgatac gttagaaaac agagaattga
     5041 ttaaagtaca tgtactacaa aataactttg atgataaaaa agaattagct gaaacattaa
     5101 gcgaagctac gcgtagtgaa ttagtgcaag tgattggatc tatgatagtg atttatagag
     5161 aatctaaaga aaataaagaa attgaattgc cataataatg aaaaagatag tactttacgg
     5221 cggtcagttt aaccctatcc atactgcaca tatgatagta gctagcgaag tatttcatga
     5281 attacagcca gatgaatttt attttttacc tagttttatg tctccattga aaaagcacaa
     5341 taattttata gatgttcagc acagattaac aatgatacag atgattatcg acgagcttgg
     5401 ttttggagat atttgcgacg atgaaattaa acgtggtggt cagagttata cctatgacac
     5461 gatcaaggca ttcaaggagc aacacaaaga cagtgagttg tactttgtta ttgggacgga
     5521 tcagtataac caactagaga aatggtatca aattgaatac ttaaaagaaa tggttacttt
     5581 tgtagttgta aatcgagaca aaaatagtca aaatgttgaa aatgctatga ttgcaattca
     5641 gatacctagg gtagatataa gttcgacaat gattcgacaa agagttagtg aagggaaatc
     5701 tatccaagtt cttgttccta aatccgttga aaactatatt aagggggaag gattatatga
     5761 acattgaaaa agcaaaacgg cttgcaaaag agaaactgcc agagaaacgt tataatcatt
     5821 ctttaagagt tgcagaaaca gctattaaat tggcagaaat ttatgatgga gacactagta
     5881 aagtagaatt agcaggtgta ttacatgatt tctgtaaata tgatgattta ggtaaaatgt
     5941 atcaaattgt tcgacaatac gaattaggta atgatctact gagttatggt agtgaaatat
     6001 tgcatggccc tgtgtgtgca gcaatcatgg aacatgaata tggtatcaat gatgaggaag
     6061 tattaatggc tatcaaatac catactactg gacgtcaaca aatgacaaaa actgaaaaac
     6121 tgatttttat tgcagattac atcgaacctg gaagaacaat cccaggagtt gatgatattc
     6181 gagatatggc atacaatcaa ggtagtttag ataagacaat ttatgaaatt tctaaacgca
     6241 cagtactatt tttaatacag aaagatatta cggtatataa taagacgatt gactgtttaa
     6301 attattataa ctatagtgac gaaagaataa aggatgatta aatgaattca caagaattat
     6361 tagcaattgc tgtggatgca attgacaata aaaaaggcga agatacgatt tctttagaaa
     6421 tgaaaggtat cagcgatatg acagattatt ttgttgtaac gcacggaaat aatgaacgac
     6481 aagttcaagc gattgctaga gcggtgaaag aagtagccaa tgaacaaaat atagaagtaa
     6541 aacgtatgga aggatacaat gaagcgcgtt ggatattaat tgacttagct gatgttgtgg
     6601 tacatgtttt ccataaagac gaaagaaatt attataatat tgaaaagtta tatcaagatg
     6661 caccattaga atcatatagt caggttgcgt attaattatg tcgcaatatg cagaaatgag
     6721 cctagtgtac gatcaattga ctcaagatca accatatgaa aaatggtttg aaattgtaaa
     6781 aaatcactgc aaagatgaat caaatatttt agatattgga tgcggtactg gtagtttaac
     6841 agttcaatta gaagctttag gtaatgttac tggtatggat ttaagtgttg atatgttgac
     6901 tgttgcagct caaaaatcag caaatgtaaa ttggcttgaa ggagatatga catcttttga
     6961 tttgcaacaa caatttgata tcatcactat tttttgtgac tctctaaatt atttgcaaga
     7021 tgaaactgca gtgattgaaa cattcatcaa tgtttatcat catctgactg attcaggtgt
     7081 atttattttt gatgtacata ctgtttataa aatgatgact ttgtttaata atcaaagtta
     7141 tattgatgat aaaggggaca tttttttagc atgggatgct gtgcaaggag atttaccttt
     7201 aagtgtttat catgatatga catttttcat tcgacatgaa gatgaaacat attcaagatt
     7261 tgatgagtct cattttcaga gaacatttga tgaaaagaca tatttgtctt ggttagctca
     7321 agttggtttt aaacatgttg aaacatttac tgattttaat atagatgaac ataatgaaga
     7381 tgcagaaaga ttgtttttca ttgcgaaaaa ataaaagtaa aaccactcgc tcagacgtgt
     7441 ataaagttga ggagtggttt tattgtatca atttttatta cgctataaag attttttaac
     7501 tcaatggaag ttatatatta taagtgctgt tgttttaatt atggtattaa ttggttttat
     7561 attctggaga caagatgatt atacttcaag aaattttgaa aataaagata atgctctgaa
     7621 acaaagcact agtgaaaata gtagtttgtc caaagtagaa gatgtccagg tcaaagatgg
     7681 agataattcc aaaaataagg gtcctgtata tgtcgatgta aaaggtgctg ttaaacatcc
     7741 taatgtttat aaaatgacat ctaaggatag agtagttgat ttacttgata aagcacaatt
     7801 attggaggat gcagatgtaa gtcaaattaa tttgtctgaa aaattaacag atcaaaaaat
     7861 gattttcata cctcataaag gacaaaagaa tgttgaacca caaattggag taaacagtgt
     7921 gcatgtaaaa aatgggaaca caaataatac taaagtaaat ttaaataccg catctgtatc
     7981 agaattgatg tctgttcctg gagtagggca agctaaagct aatgcaattg ttgaatatcg
     8041 caaccaacaa ggtgcatttc aagaaattga cgatttgaaa aaagtaaaag gttttggaag
     8101 taaaactttt gataaactga aatcttattt cacgatataa tggattattt taattgcaaa
     8161 ttttattttt tgtgattaat cttatattaa tagaaaatgt tactaaattt taagtatgga
     8221 aggtgaacat cttggaaaga atcaaatggg aagaatattt tatggcacaa agtcatttgc
     8281 tagcattacg ttcaacttgt caaagattat ctgtaggtgc aacgattgtt aaggataatc
     8341 gtattattgc tggtggttat aatggctctg tagctggcga ggtgcattgt atagatgaag
     8401 gatgtttaat tgaagatgga cattgtatca gaacgataca tgcagaaatg aatgctttat
     8461 tacaatgtgc aaaacaaggt gtatctactg aaggtgcaac aatctatgtt actcattttc
     8521 catgcctaaa ttgtacaaag tcaattattc aagcaggtat aaagcgtatc tactatgcag
     8581 aagattatca taaccatgaa tatgcaacta aattactcaa acaatctggt attgaattta
     8641 aaaaaattcc attttcacca gaatatgttg ctaaatatct gactaaaggt taatgttttg
     8701 ctgtatgtcg cgttatcaat gattgtagga gtgctttgga attctagcaa agtgctctct
     8761 acatctcttt tcattttact tttgtatatt acttatcgta aaaataaaat cgtttatgcc
     8821 cctatttctc tctttttaat cattttctcc tcatggtatt tacattattc acaacaagca
     8881 atatttaatt atatcaatta tattgaacgt aattctcagt ttaatgagcg tgctcaagta
     8941 atccaaattc aacgtcaagg tagtgacaca tataaaggta ggttgagttt aaaaaatgaa
     9001 atatatcctt tctttttaac aaataaaaag aattttgatt taaagaaaat tgaaagtcgt
     9061 aattgtattg ttaaaggaca attcaaagtt aatgacaata agtttgtaac tcttaaatta
     9121 caaagtatag ttgtacaaag ctgcctagaa tcgaaccggt ctaatttaat tgagaaacat
     9181 aaacagttta taatgaatcg aatttatgat tcgggtatta agtttccgga tcgtattatg
     9241 gcattgatta ctggtgacgt aaaagaaatt aatgagcaat ttaaggaacg tgttaaagag
     9301 ataggtatat atcatttgct ggcagttagt ggctcgcata tagctgcaat tgtattctta
     9361 atttacaaac ctttaaaacg attaaattta cctttatttg tcattaaagg aattacaatc
     9421 attgtattag ctttatttgc tcaatacaca aattatgcac ctagtgctgt aagagctata
     9481 ataatgacaa ctcttgtact gcttattact aagcaaatta aaataaaggg tattcagcta
     9541 ttagcatttg catttataat tatgtttatt ttaaatccac tagttgttta tgatattgga
     9601 tttcaatttt cattcatcat ttcatttttt attatgctac tttttccttt tttacagcaa
     9661 ttgtcaaagt tacaatcatt attcataatt acgtttattg cacaattagc ttcatttatc
     9721 gttgccattc caaactttca tcaacttcaa tgggtgggat ttttatctaa tttgattttt
     9781 gtaccgtact attcgattat attgtttccg ctatctattt tattctttat tacaagtcat
     9841 tttattgtgg gattaacgcc gctaaattac ttggttgacc taagttttaa ttttcatgac
     9901 tggttactag acctattcac aaga
//
LOCUS       DBNENW010000095         9912 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNENW010000095 DBNENW010000000
VERSION     DBNENW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.096"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..3215
                     /locus_tag="AC1LW8_001851"
     CDS             <1..3215
                     /locus_tag="AC1LW8_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIP4773675.1"
                     /translation="QNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENERK
                     EIEKLENQRRDISVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKARK
                     KEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKKQN
                     KDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRENI
                     KKWFGNAWDGVKTKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSSWSSAKSSV
                     GYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTSKW
                     FSNAYKSAKGWLTDMANKSRSKWDNISSTAWSNAKSVWKGTSKWFGNSYKSLKGWTGD
                     MYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNVAN
                     KAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFAVL
                     NDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGVFP
                     KFHGGTKKKKWMEQVTENLGKKAGGFGSKAKNTAHNIKKGAEEMVEAAGDKIKDGASW
                     LGDKIGDVWDYVQHPGKLVKKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKVKS
                     WFEDFGGGGDGSYLFDHPIWQRFGSYTGGLNFNGGRHYGIDFQMPTGTNIYAVKGGIA
                     DKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVRGA
                     HLHFQLMRGSHPGNDTAVDPMKWLKSLKGGGGKVGGSGYENAKRAILRAQSILGGRYR
                     SDYITTQMLRVAKRESNYQADAINNWDSNARAGTPSKGMFQMIEPSFRAFAKPGHGNI
                     YNPTDEAISAMKYIVAKYGWGGFKRAGDYAYANGGLITKHQIAEVGEGDKPEMVIPLT
                     RRKRAMQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLTDKGNKLTDALIQT
                     VSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            3215..4039
                     /locus_tag="AC1LW8_001852"
     CDS             3215..4039
                     /locus_tag="AC1LW8_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HIP4773676.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNGSGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            4048..5631
                     /locus_tag="AC1LW8_001853"
     CDS             4048..5631
                     /locus_tag="AC1LW8_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase tail family protein"
                     /protein_id="HIP4773677.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIKTTDINYSNDFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            5631..5921
                     /locus_tag="AC1LW8_001854"
     CDS             5631..5921
                     /locus_tag="AC1LW8_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773678.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDNLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            5937..7847
                     /locus_tag="AC1LW8_001855"
     CDS             5937..7847
                     /locus_tag="AC1LW8_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773679.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTIQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSWIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            7847..9313
                     /locus_tag="AC1LW8_001856"
     CDS             7847..9313
                     /locus_tag="AC1LW8_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HIP4773680.1"
                     /translation="MSNLEKSVNINLENTAHYENISNLDITFRTGESDSSVLLFNITK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRITDLENKAQTYSRAFDEQ
                     KRYMDDKHEAFKQSVNSGGLVASGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRGASTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEVGSWSDW
                     SELALNDYNTPFETVQNAQSKANTAESNAKLYTDDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGNPFGGGKISLNPSNLPDNDGDGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSRRGSGANANRGTINKIIGVRK"
     gene            9313..9702
                     /locus_tag="AC1LW8_001857"
     CDS             9313..9702
                     /locus_tag="AC1LW8_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HIP4773681.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            9695..9859
                     /locus_tag="AC1LW8_001858"
     CDS             9695..9859
                     /locus_tag="AC1LW8_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HIP4773682.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
BASE COUNT         3701 a         1353 c         2055 g         2803 t
ORIGIN      
        1 agcaaaacat tttaactcga actaaagaaa aaaatgactt gcgaattaaa aaagagcaag
       61 aactcaatca gaaaatcaaa gaattgaaag aaaaagcttt aagtgatggt cagatttcag
      121 aaaatgaaag aaaagaaatt gaaaagcttg aaaatcaaag acgtgacatc tctgttaaag
      181 aattgagtaa gactgaaaaa gagcaagagc gtattttagt aagaatgcaa agaaacagaa
      241 atgcttattc aatagacgaa gcgagcaaag caattaaaga agcagaaaaa gcaagaaaag
      301 caagaaaaaa agaagtggac aagcaatatg aagatgatgt cattgctata aaaaataacg
      361 tcaacctttc taagtctgaa aaagataaat tgttagctat tgctgatcaa agacataaag
      421 atgaagtaag aaaagcaaaa tctaaaaaag atgctgtagt agatgttgtt aaaaagcaaa
      481 ataaagatat tgataaagaa atggatttat ccagtggacg tgtatataaa aatactgaaa
      541 agtggtggaa tggtcttaaa agttggtggt ctaacttcag agaagaccaa aagaagaaaa
      601 gtgataaata cgctaaagaa caagaagaaa cagctcgtag aaacagagaa aatataaaga
      661 aatggtttgg aaatgcttgg gacggcgtaa aaactaaaac tggtgaagcc tttagtaaaa
      721 tgggcagaaa tgctaatcat tttggcggcg aaatgaaaaa aatgtggagt ggaatcaaag
      781 gaattccaag caaattaagt tcaagttgga gctcagccaa aagttctgta ggatatcaca
      841 ctaaggctat agctaatagt actggtaaat ggtttggaaa agcttggcaa tctgttaaat
      901 cgacaacagg aagtatttac aatcaaacta agcaaaagta ttcagatgcc tcagataaag
      961 cttgggcgca ttcaaaatct atttggagag gcacatcaaa atggtttagc aatgcatata
     1021 aaagtgcaaa gggctggcta acggatatgg ctaataaatc gcgctcgaaa tgggataata
     1081 tttctagtac agcatggtcg aatgcaaaat ccgtttggaa aggcacatcg aaatggtttg
     1141 gtaactcata caaatcttta aaaggttgga ctggggatat gtattcaaga gcccacgatc
     1201 gttttgatgc aatttcaagt tcggcatggt ctaacgctaa atcagtattt aatggtttta
     1261 gaaaatggct atcaaaaaca tatgattgga ttagagatat tggtaaagac atgggaagag
     1321 ctgcggctga tttaggtaaa aatgttgcta ataaagctat tggcggtttg aatagcatga
     1381 ttggcggtat taataaaata tctaaagcca ttactgataa aaatctcatc aagccaatac
     1441 ctacattgtc tactggtact ttagcaggaa agggtgtagc taccgataat tcgggagcat
     1501 taacgcaacc gacatttgct gtattaaatg atagaggttc tggaaacgcc ccaggtggtg
     1561 gagttcaaga agtaattcac agggctgacg gaacattcca tgcaccccaa ggacgagatg
     1621 tggttgttcc actaggagtt ggagatagtg taataaatgc caatgacact ctgaagttac
     1681 agcggatggg ggtttttcca aaattccatg gtggtacgaa aaagaaaaaa tggatggaac
     1741 aagttactga aaatcttggt aaaaaagcag ggggcttcgg ttctaaagct aaaaacacag
     1801 ctcataatat caaaaaaggt gcagaagaaa tggttgaagc ggcaggcgat aaaatcaaag
     1861 atggtgcatc ttggttaggc gataaaatcg gcgatgtgtg ggattatgta caacatccag
     1921 ggaaactagt aaaaaaagta atgtcaggtt taaatattaa ttttggaggc ggagctaacg
     1981 ctacagtaaa aattgctaaa ggcgcgtact cattgctcaa aaagaaatta gtagacaaag
     2041 taaaatcgtg gtttgaagat tttggtggtg gaggcgatgg aagctatcta tttgaccatc
     2101 caatttggca aaggtttggg agctacacag gtggacttaa ctttaatggc ggtcgtcact
     2161 atggtatcga ctttcaaatg cctactggaa cgaacattta tgctgttaaa ggcggtatag
     2221 ctgataaagt atggactgat tacggtggcg gtaattctat acaaattaag accggtgcta
     2281 atgaatggaa ctggtatatg catttatcta agcaattagc aagacaaggc caacgtatta
     2341 aagctggtca actgataggg aaatccggtg ctacaggtaa tttcgttaga ggagcacact
     2401 tacatttcca attgatgaga ggttcacatc caggtaatga tacagcagta gatcctatga
     2461 aatggttgaa gtcacttaaa ggtggcggtg gtaaggttgg cggaagcgga tacgagaatg
     2521 caaaaagagc tattctaaga gcacaatcaa ttttaggtgg acgatataga tctgactata
     2581 tcactactca gatgttaaga gtagccaagc gtgaaagtaa ctatcaggca gatgctatta
     2641 acaactggga ttccaacgca agagcaggta caccgtctaa aggtatgttc caaatgattg
     2701 agccttcatt tagagctttt gctaagcccg gacatggaaa tatttataat cctactgatg
     2761 aggcgatttc agcaatgaaa tacattgttg ctaagtacgg ttggggaggc tttaaacgcg
     2821 caggagatta tgcctatgcg aatggaggtc ttataactaa acatcaaatc gctgaagtgg
     2881 gagaaggaga taaaccagaa atggttattc cgttgacacg acgcaaaaga gcaatgcaat
     2941 taactgaaca ggttatgcgc atcatcggta tggatggcaa gccaaataac atcactgtaa
     3001 ataatgatac ttctacggtt gaaaaattat tgaaacaaat tgttatgcta acggataaag
     3061 gaaataaatt aacagatgca ttgattcaaa ctgtttcttc tcaggataat aacttaggtt
     3121 ctaatgatgc aattagaggt ttagaaaaaa tattgtcaaa acaaagtggg catagagcaa
     3181 atgcaaataa ttatatggga ggtttgacta attaatgcaa tcttttgtaa aaatcataga
     3241 tggttacaag gaagaagtaa taacagattt taatcagctt atatttttag atgcaagggc
     3301 tgaaagtcca aacaccaatg ataacagtgt aactattaat ggagtagatg gtattttacc
     3361 gggcgcaatt agttttgcgc ctttttcatt agtattaagg tttggctatg atggtataga
     3421 tgttatagat ttaaatttat ttgagcattg gtttagatct gtgtttaatc gcagacatcc
     3481 ttattatgtt attacttctc aaatgcctgg tgttaaatat gcagtgaata cagctaatgt
     3541 tacatctaat ttaaaagatg gttcttcaac tgaaattgaa gtaagtttaa atgtttataa
     3601 agggtattct gaatcagtta attggaccga tagcgagttc ttattcgact ctaattggat
     3661 gtttgaaaat ggaattcctc ttgatttcac acctaaatat actcatacat caaatcaatt
     3721 tactatttgg aacggttcta ctgatacgat aaatccacga ttcaagcacg atttgaaaat
     3781 attaattaat ttaaatggga gtggaggatt tgaactggtt aactatacaa caggtgatat
     3841 ttttaagtac aacaaaagta tagataaaaa cactgatttt gttttagatg gtgtgtatgc
     3901 atatcgagat ataaatagag tgggaattga tacaaataga ggcattataa cattagcgcc
     3961 aggtaaaaat gaatttaaga ttaaaggaga cgtcagtgat attaaaacta catttaagtt
     4021 tccttttatt tataggtagg tgatttaatg gattatcatg atcatttatc agtaatggat
     4081 tttaatgaat tgatttgtga aaatttacta gatgtagatt atggttcttt taaagaatat
     4141 tatgaactga atgaagctag gtacatcacc tttacagttt atagaactac tcataatagt
     4201 tttgtttttg atttattgat ttgtgaaaac ttcataatat atcatggtga aaaatataca
     4261 attaagcaga cagcgccaaa ggttgaaggt gataaagttt ttattgaagt tacggcatat
     4321 cacataatgt atgaatttca aaatcactca gtggaatcaa ataagcttga tgacgacagt
     4381 agcgaaactg gtaaaacgcc agaatactct ttagatgagt acttaagata tggatttgca
     4441 aatcaaaaaa cttcggtcaa aatgacctat aaaataattg gagattttaa gcgaaaagta
     4501 ccgattgacg aattaggtaa caaaaacggc ttagaatact gtaaagaagc ggtagacctg
     4561 tttggctgta taatttaccc aaatgataca gagattggtt tttattctcc tgaaacattt
     4621 tatcaaagaa gcgagaaagt gattcgatat caatataata ctgatactgt atctgcaact
     4681 gtcagtacgt tggaattaag aacagctata aaagtttttg ggaaaaagta cacagccgag
     4741 gaaaagaaaa attataatcc tattaaaaca actgacatta attactcaaa tgatttcata
     4801 aaagaaggta cttatcgcac agaaacaatt ggttctaaag caactattaa ctttgattgc
     4861 aagtatggta atgaaacagt tagatttaca atcaaaaaag gttcccaagg tggaatatat
     4921 aagttgattt tagacggcaa gcaaattaag caaatttctt gttttgctaa gtcggttcag
     4981 tctgaaacaa tagatttaat aaaaaatatt gataaaggca agcacgtttt agaaatgata
     5041 tttttaggag aagaccccaa aaatagaatt gatatctctt caaataaaaa agctaagcct
     5101 tgtatgtacg ttggaactga aaaatcaaca gtcttaaatt taattgctga caattcaggt
     5161 cgcaatcaat acaaagcaat tgttgactac gtcgcagata gtgcaaagca gtttgggatt
     5221 cgatatgcta atacgcaaac aaatgaagat atcgaaacac aggataagct gttagaattt
     5281 gcaaaaaagc aaataaatga tactcctaag actgaattag atgttaatta tataggttat
     5341 gaaaaaatag agccaagaga tagcgtattt tttgttcatg aattaatggg atataacact
     5401 gaattaaagg ttgttaaact tgataggtca catccatttg taaacgcaat agatgaagtg
     5461 tctttcagca atgaaataaa agatatggta caaattcaac aagcacttaa cagacgagtt
     5521 attgcacaag ataatagata taactatcaa gcaaatcgta taaatcattt atacactagt
     5581 actttgaatt ctcctttcga gacaatggat atagggagtg tattaatata atggcaacag
     5641 aagaagttaa aatcaaagcg ctacttgaaa acgataaaca gtactttcca gctacacatt
     5701 ggaaagctat aaatgggata ccttatgcag gcagtagtga tattgatgga ttgcctcaag
     5761 acggtatcat ttcggtagat gataaaaata aattagataa tttaaaaata ggcgaagcag
     5821 gaattattca aaatagcatt gtacagaaat ccccaaacgg taaattgtgg aaaataacag
     5881 ttgacgatag tgggaaactt ggtacagtgc tattttatta gaaaggaagg tgcattatgg
     5941 aaaatttgta tttaataaag gatttgggag ctttagcagg tcgagattat agagctaaag
     6001 aaattcaaaa cctgcaaaga atagagcaat ttgcgcttgg cttgacaaca gagtttaagt
     6061 tgcatcagaa agctaaaaca attcaacact tcgctgagca aatttattat aatggtagat
     6121 cgcaagcagc agtaaacaaa tctttacaaa gtcaaattaa cgcacttgtt gtggcaccac
     6181 gtaataacag tgctaatgag attgttcaag ctcgagttaa tgtaaacggc gaaacctttg
     6241 acacattaaa agaacattta gacgattggg aaaccaaaac tcaaattaat aaagaggaaa
     6301 ctataagaga attaaataag accaaacaag aaattcttga tatcgagtat cgttttgaac
     6361 ctgataagca agaattttta tttgtgacag aacttgcacc tcttacaaat gcagtaatgc
     6421 aatccttctg gtttgataat agaacaggca tagtatacat gacacaagct agaaataatg
     6481 gctatatgct aagtcgttta agacctaatg gtcaatttat agacagctca tggattgtag
     6541 gtgggggtca tggtacacat aacggttata gatatattga tgatgagtta tggatttata
     6601 gttttatctt aaatggtaat aatgagaata cattagttcg tttcaagtat acgcctaatg
     6661 tggaaattag ctatggcaag tatggtatgc aagatgtatt tacaggacac ccagaaaaac
     6721 cctacatcac ccctgtcata aatgaaaaag aaaataaaat tctatacaga attgagagac
     6781 ctagaagtca gtgggaactt gaaaactcaa tgaattatat agagataaga agtttagacg
     6841 atgttgataa aaatattgat aaagttttgc ataaaatcag tatccctatg agactaacaa
     6901 acgaaaccca accaatgcag ggtgtgactt ttgatgaaaa atacttgtat tggtatacag
     6961 gagacagtaa tccaaataat agaaactatt taacggcttt cgatttagaa acaggagaag
     7021 aagcgtatca ggttaatgct gactatggtg gaacactaga ttcatttcct ggcgaatttg
     7081 cggaagcaga aggtttgcaa atatactatg acaaagatag tggtaaaaaa gctttgatgc
     7141 taggtgttac tgtcggtggt gatggaaata gaacacatcg tattttcatg attgggcaaa
     7201 gaggtatttt agaaatactt cactcaagag gcgttccttt tatcatgagt gacacaggtg
     7261 gtagagttaa acctttacca atgaggcctg ataaacttaa gaatcttggg atgttaacag
     7321 agccaggtct ttactattta tacactgatc atacagttca aatcgatgat ttcccattac
     7381 caagagaatg gcgtgatgca ggttggttct tggaagttaa gccaccacaa actggcggtg
     7441 atgtaattca gatattgacg cgtaatagtt atgcaaggaa tatgatgact tttgaaaggg
     7501 tgctttctgg aagaactgga gacatttcgg actggaatta tgtgcctaaa aatagtggta
     7561 aatgggagag agtaccttca ttcatcacaa aaatgtcaga tattaacata gtaggcatgt
     7621 cgttttattt aactacggat gatacaaaac gttttacaga ttttccaact gaacgtaaag
     7681 gggtagctgg ttggaactta tatgtagaag cttcaaacac aggtggcttt gttcataggc
     7741 tagttcgtaa tagtgttaca gcatctgctg agatactatt gaaaaattat gatagtaaaa
     7801 caagttcagg gccatggact ttacacgaag ggagaattat aagttaatga gtaatttaga
     7861 gaaatctgta aatattaatt tagagaacac agcgcattat gaaaatattt caaatctaga
     7921 tataactttt agaacaggag agagtgactc ttctgttctt ctttttaata tcactaaaaa
     7981 taatcaaccg ttattattga gtgaagaaaa tatcaaagca cgaatagcga ttcgaggtaa
     8041 aggagtcatg gtagttgctc cactagaaat attagatcca tttaaaggta ttttaaaatt
     8101 tcaattacct aatgatgtaa ttaaacgaga tggaagttat caagctcaag tttcggttgc
     8161 agaattaggt aattcagacg tggtagttgt cgagagaact atcacattta acgttgaaaa
     8221 aagtttgttt agcaagattc cctctgaaac aaaactacac tatattgttg agtttcaaga
     8281 attagaaaaa actattatgg atcgcgcgaa agcaatggac gaggctataa aaaatggtga
     8341 agattatgcg agcctgattg aaaaagctaa agaaaaaggt ttgtcagata ttcaaatagc
     8401 aaaatcttca agtatagatg aattaaagca acttgcaaat agccgtataa ccgatttgga
     8461 aaataaagcg caaacttatt caagagcatt cgatgagcaa aagcggtata tggatgataa
     8521 acatgaagct tttaagcaat ctgtgaatag tggtggttta gtcgcaagtg gttcaacttc
     8581 aaattggcaa aaagctaaga ttactaaaga tgatggtaag ataatgcaga ttactggatt
     8641 tgattttaat aatccagaac aaagaatagg cgattcgact caatttattt atgtttcgca
     8701 agctataaat tatccaagag gcgcaagcac taatggtact gtcgaatatt tagtagtaac
     8761 ttccgactac aaacgtatga catatcgtcc aaatggtaca aataaagtat ttgttaagag
     8821 aaaagaagtg ggttcgtggt ctgattggtc agaattagcg cttaatgatt acaacacacc
     8881 ttttgaaact gttcaaaacg cgcaatcaaa ggctaacacg gctgaaagca atgccaaact
     8941 atacacagat gacaagttta ataaaagata ttcagttatt tttgatggga cggcaaatgg
     9001 cgttggctca acattatatc ttaatgaaag tttagatcaa tttattttgt taatttttta
     9061 tggaactttt ccaggtggag attttactga gtttggcaac ccctttggtg gcggaaaaat
     9121 ttcattgaac ccatcaaatt taccggataa tgacggtgac ggtggaggcg tttatgagtt
     9181 tggattaact aaatctagtc gtacatcatt aactatatca aacgatgttt attttgactt
     9241 aggaagtcga agaggttctg gtgcaaatgc aaatagagga acaatcaaca aaattatagg
     9301 agtgagaaaa taatgcaaat attagttaac aagcgtaatg agataatttc atacgctatc
     9361 attggtggct ttgaagaagg tattgatatt gaaaatttac cagaaaattt ctctcaagtt
     9421 tttagaccta aagcctttaa atattcaaat ggggaaatag tttttaacga agattattca
     9481 gaagaaaaag atgacttgca tcaacagatt gacagtgaag aacaaaacac agtcgcttct
     9541 gatgacatct tacgaaaaat ggttgctagt atgcagaaac aagttgttca aagtacaaag
     9601 ttatcgatgc aagttaataa gcaaaatgca ctaatggcaa aacaacttgt gacacttaat
     9661 aaaaaattag aagaggttaa aggagagact gaaaatgctt aaattaattt caccaacatt
     9721 cgaagatatt aaaacatggt atcaattgaa agaatatagt aaagaagata tagcgtggta
     9781 tgtagatatg gaagttatag ataaagagga atatgcaatt attacaggag aaaagtatcc
     9841 agaaaatcta gagtcatagg ttataatctt atggcttttt aatttgaata aagtgggtgg
     9901 tgtaatgttt gg
//
LOCUS       DBNENW010000096         9746 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.097, whole genome shotgun sequence.
ACCESSION   DBNENW010000096 DBNENW010000000
VERSION     DBNENW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.097"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(19..94)
                     /locus_tag="AC1LW8_001859"
     tRNA            complement(19..94)
                     /locus_tag="AC1LW8_001859"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:Ala,seq:tgc)
     gene            complement(118..191)
                     /locus_tag="AC1LW8_001860"
     tRNA            complement(118..191)
                     /locus_tag="AC1LW8_001860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Pro,seq:tgg)
     gene            complement(212..288)
                     /locus_tag="AC1LW8_001861"
     tRNA            complement(212..288)
                     /locus_tag="AC1LW8_001861"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Arg,seq:acg)
     gene            complement(294..382)
                     /locus_tag="AC1LW8_001862"
     tRNA            complement(294..382)
                     /locus_tag="AC1LW8_001862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(346..348),aa:Leu,seq:taa)
     gene            complement(390..464)
                     /locus_tag="AC1LW8_001863"
     tRNA            complement(390..464)
                     /locus_tag="AC1LW8_001863"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Gly,seq:gcc)
     gene            complement(498..570)
                     /locus_tag="AC1LW8_001864"
     tRNA            complement(498..570)
                     /locus_tag="AC1LW8_001864"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Lys,seq:ttt)
     gene            complement(577..652)
                     /locus_tag="AC1LW8_001865"
     tRNA            complement(577..652)
                     /locus_tag="AC1LW8_001865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(617..619),aa:Thr,seq:tgt)
     gene            complement(669..744)
                     /locus_tag="AC1LW8_001866"
     tRNA            complement(669..744)
                     /locus_tag="AC1LW8_001866"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(709..711),aa:Val,seq:tac)
     gene            complement(757..871)
                     /gene="rrf"
                     /locus_tag="AC1LW8_001867"
     rRNA            complement(757..871)
                     /gene="rrf"
                     /locus_tag="AC1LW8_001867"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1429..2916)
                     /gene="lysS"
                     /locus_tag="AC1LW8_001868"
     CDS             complement(1429..2916)
                     /gene="lysS"
                     /locus_tag="AC1LW8_001868"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIP4773683.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDDEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2969..3061)
                     /locus_tag="AC1LW8_001869"
     CDS             complement(2969..3061)
                     /locus_tag="AC1LW8_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773684.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3455..3931)
                     /gene="folK"
                     /locus_tag="AC1LW8_001870"
     CDS             complement(3455..3931)
                     /gene="folK"
                     /locus_tag="AC1LW8_001870"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIP4773685.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3928..4293)
                     /gene="folB"
                     /locus_tag="AC1LW8_001871"
     CDS             complement(3928..4293)
                     /gene="folB"
                     /locus_tag="AC1LW8_001871"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIP4773686.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4271..5074)
                     /gene="folP"
                     /locus_tag="AC1LW8_001872"
     CDS             complement(4271..5074)
                     /gene="folP"
                     /locus_tag="AC1LW8_001872"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIP4773687.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5290..6222)
                     /gene="cysK"
                     /locus_tag="AC1LW8_001873"
     CDS             complement(5290..6222)
                     /gene="cysK"
                     /locus_tag="AC1LW8_001873"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIP4773688.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(6401..7282)
                     /gene="hslO"
                     /locus_tag="AC1LW8_001874"
     CDS             complement(6401..7282)
                     /gene="hslO"
                     /locus_tag="AC1LW8_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIP4773689.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(7511..9604)
                     /gene="ftsH"
                     /locus_tag="AC1LW8_001875"
     CDS             complement(7511..9604)
                     /gene="ftsH"
                     /locus_tag="AC1LW8_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIP4773690.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2932 a         2078 c         1355 g         3381 t
ORIGIN      
        1 catcgtgtaa aaaataaatg gtggagacta gcgggatcga accgctgacc tcctgcgtgc
       61 aaagcaggcg ctctcccagc tgagctaagc ccccataaaa tggaatttaa gaagtaatcg
      121 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
      181 gctacttccc gttattcaat taatttaaaa atggcgcgcc cgataggagt cgaacccata
      241 acctcttgat ccgtagtcaa acgctctatc caattgagct acgggcgcta aaatggtgcc
      301 gaggaccgga atcgaaccgg tacggtgatc tctcaccgca ggattttaag tcctgtgcgt
      361 ctgccagttc cgccaccccg gcaaaataat ggagcagaag acgggattcg aacccgcgac
      421 cccaaccttg gcaaggttgt attctaccgc tgaactactt ctgcattatt ttgctaaata
      481 taaaaattaa atcgtaatga gccatagagg attcgaacct ctgaccctct gattaaaagt
      541 cagatgctct accagctgag ctaatggctc aaaagatggt gccggccaga ggacttgaac
      601 ccccaaccta ctgattacaa gtcagttgct ctaccaattg agctaggccg gctatatcta
      661 agaataaatg gtggagaatg acgggttcga accgccgacc ctctgcttgt aaggcagatg
      721 ctctcccagc tgagctaatt ctccgatttg tcatttgcct ggcaacgttc tactctagcg
      781 gaacgtaagt tcgactacca tcgacgctaa ggagcttaac ttctgtgttc ggcatgggaa
      841 caggtgtgac ctccttgcta tagtcaccag acaaaacaac ttacttattc atgatacacg
      901 cttaattaat aaggtgcaat agttaatttt acactttgat gtaaactttg tcacatcagc
      961 ttttagtgtg tttcgtctgt ttttgacgac ttttatatat tacttcattc aattgataaa
     1021 gtcaacactt ttaatcaact tttttattct tcaaaactga catttctcgt gccctctaat
     1081 tcataacact tttagtattc tatcatacac atatgtacaa aaccatacac attctataca
     1141 attttaaagt acttttatag ctgattaaga taaattatac taatatagca atttttgtta
     1201 taagatatct caatgtctat cgttaacatt tctcatccga actactctat atacgtatcg
     1261 tcattttcat ctatcagcca actaccttgt ttaacagtca aaaatagcac cactatcgtt
     1321 aactattact atcaaaaaca ttcaaacacg aactaattac cataattaag aaagccctcg
     1381 catacacatg ttgtatacac gagagctctt actaacaatc aacgtcattt atttttgtct
     1441 catataaggg aataataata cgtctctgat tgatggagag tcagttaata acataaccaa
     1501 tctgtcaata ccgataccaa gaccacctgt cggaggcata ccatattcta acgcttcaat
     1561 gtaatcttca tccatttcat gcgcttcatc attaccttgc gctttttcaa caagttgcgc
     1621 ttcaaaacga cctttttgat caataggatc atttaattca gtaaatgcat ttgcatgctc
     1681 tctacctaca atgaacaatt cgaaacgatc agtaaatcta ggatcttcag gatttttctt
     1741 cgctaaaggt gaaatttcag tcggatgacc atagataaac gttggctgaa taagtgtttc
     1801 ttcaactttt tgctcaaaga attcatttaa aatatgacca tatttcattg tatctttaat
     1861 ttcaatacca tgttctttag ctaaagcttt agcttcatca tcacttttaa cttcataaaa
     1921 atctacacca gtagcttctt ttacagcatc aacaatatgc aaacgagtcc aagcagattc
     1981 taaatcaatc gtttccccat tgtattgtac ttttgcagaa cctaacactt cattggcaat
     2041 atgtctcacc atagattctg ttaaatccat aatgtcatga taatctgcat atgcttcata
     2101 taattcaatc attgtgaatt cagggttatg tctagttgat acaccttcat tacggaatac
     2161 tctaccaatt tcatatactt tttcaagtcc accgacaatt aaacgtttta aatgcaactc
     2221 aatagcaata cgcatgtata acgttgcatc taatgcatta tgatgtgtta caaatggtct
     2281 agcagctgct ccaccagcaa tttggtgcat cataggtgtt tctacttcca agaaaccttt
     2341 attatttaaa taattacgca tttcttgaat gattttacta cgattaataa atgtacgagt
     2401 gctatcttcg ttcgtaatta aatctaaata tctttgacga tatctctgtt caatatcctg
     2461 taaaccgtgg aatttatccg gtaatggtcg caatgattta gttagtagcg tgaatttctt
     2521 cgctttaacc gataattcgc cagtatttgt tttgaacatt acaccttcaa caccaacgat
     2581 atcgcctaaa tcagcatttt tccataaatc aaattcgtca tcgccaactt gatctttacg
     2641 aacgtaaatt tgaatttgtc cagctaagtc ctgaacgtgt gcaaatcctg ctttaccttt
     2701 accacgctta gtcattaatc gtccagctat agcgacatga ctatccgctt ctttttctac
     2761 caattcttct ttagaatact ggtcccactc ttctttcaaa tcactagata aacctgaacg
     2821 gtcaaattta gaaccaaacg ggtctatacc aagatcatat aattcttgta atttttgacg
     2881 tcgaaccaac atttggtcat tcatttcttc tgacataact ttctctcctt taacttgttg
     2941 taatccttta gtattccaac tggtacactc aaacattttc ctttaaagtt ttataaaata
     3001 tcagacattt ttgctcaaca tacatctctt tcgatgtttc taatacttta tcattaacca
     3061 ttacacctta aagttatgat gtggcatgtt ttcttatatt cataacatca attttatcac
     3121 ttttttaatt ttacaagagc ttgtaaatgt aataaagcaa tttctgtttt cgtttatgaa
     3181 ttacttattc atttaaaata cgattatcat taatacaacc ctaagtaaat gtataaaaat
     3241 tatcttccac aaacttcaac aaaagcccaa ataaattaca gcaatttatc aaatattgct
     3301 tactttgatt ttatgaaata acttaattct aacacatact aaatcatcat atactaattc
     3361 gaaatcaaat gcatttagag ataatcaaaa tgcggaaaca tctccaataa tcaatcatct
     3421 attcccaata aatatgaatg ttctcaacaa tgcattattt atatctcttt acactgtcat
     3481 ctacaaaaac taaatctttc actttcaatt tcgaacgtgg ttctacgaca tttgctgcta
     3541 tatcatttaa tgggattaaa acaaatgcac gttcattcat tctcggatgt ggcaccgaca
     3601 gttttggtaa atctatcatt tcttctccat acaacaaaat atccacatct aaagttctag
     3661 gaccccatcg ttcctttcta atacggtgta aacattcttc tgtcttcaaa caacattcca
     3721 acagttgtaa tactgtgagt gttgtttgaa tttcaacaca caaatttaaa aagttaggtt
     3781 gctcagtata cccaactggt gctgtttcat aaatcggaga aatattagat acgttaatac
     3841 catcatattc attcaaaatc tttatagcat cgtttaactg gctttctcta tcaccaatat
     3901 tactacctaa tcctaagtat gcttgaatca tttattctcc ctcactattt cgatacctac
     3961 tccatcataa tgacccggaa tcggtgggtt ttctttagtg attctcactt tcgtttccat
     4021 tacacgatta tattgtgaat ttatacgatt tgcaatacgt tcagctagat gctcaagtaa
     4081 attaacggcc ttaccttcca taattgattt aacctcttcg aacacttcac cataatgaac
     4141 tgtatcaata acattatcag tacgcccagc ttcagctaag tctactttca aagttacatc
     4201 cactttgaaa atttgcccta tttcattttc agctgataaa gcaccatgat atccataaaa
     4261 gcgcatacct ttaagaaaga ttgtgtcttg cattttcatt ctcctttaaa aaatctatac
     4321 ctttagctaa tttagcattc aactcgacat tatgaacgcg tactgctcta acgcctttca
     4381 taataccata tgcagtcgta gctgcagtta cttcatctct ttcaaccggt gttgtatcat
     4441 aacccatcat ctctttagtg aaacgtttcc ggcttgtcgc taataaaact ggatattctg
     4501 ttgcaacaag ttcatccagt cttgccataa cttcggcttc ttcatttcta gttttagcga
     4561 aacctatacc tggatctagc caaattttat ttgaaggtat accagctatt ttagcttgat
     4621 gtgcttgtgc taacaaagat gttaacattt cttcgacaac cggttcatca cgattaccat
     4681 ttccattatg cattaaaata atttccgcgt catatttagc tacaatttgg aacatacgat
     4741 gatcatacag acccgcccat tgatcattaa tcatatcaac gcctaatttt aaacatgctt
     4801 cagcaacctc acttcgaaat gtatcgactg aaatttttac atcaaaaccg acaatagctt
     4861 caacaacagg taatactctg ttcatctctt cttctaatga aaccatttca tgacctggtc
     4921 tcgttgaaac acctccaaca tctataatgt cagcaccttc atctatcatg gctttcactc
     4981 tatttatagc tgattcaaca ttattaaatt ttccaccatc tgagaatgaa tcaggtgtga
     5041 cgtttaatat gcccataatt tttgttttag tcatgttaac caccctttaa ttatgtattg
     5101 tttaactcta ataaaattta ctcctgctat ttactagtta ttagtatgta cgtttattat
     5161 cttatcattc actggatatt tttatcagaa aaatgtttaa tttaacgctc atatttttaa
     5221 aaatatacat ggtagaaaaa aagagcaagt tatctcaaaa agataactca ctcttttaaa
     5281 tgacattaat tagtcatcga atgaatataa aggtgttgat aagtagcgtt caccattact
     5341 cggcaatact gttactactg ttttaccttt tcctaattct tttgcttttt gaatggcagc
     5401 ataaatcgca gcacctgatg aaatacctgc taaaatacct tcctctttag caactcgacg
     5461 agacatttcc atcgctgtat catttcctac tttaataata ctgtcataga tttctgtatt
     5521 caaagtgcct ggaataaatc cagcacctaa accttgtaat ttatgtggac ctggctcacc
     5581 accgctcaat actggagaag cctcaggctc tatagcaaca atttcgatgt taggatattc
     5641 ttttttcaga actttaccta caccagataa cgtaccacca gtaccaacac cagctaggaa
     5701 cgcatcgata gttttccctt caaattgttg taataactca ggacctgtag ttaactcatg
     5761 aacttcaggg ttcgcagggt tttcaaattg ttgtggctcg aagtaaccat gttcttcttt
     5821 caattcttta gcttttttaa ttgcaccttt catcgcttct gatccaggcg ttaaaactaa
     5881 ttccgcaccg tatgctttta ataaattacg acgctcttgg ctcattgttt cgggcatagt
     5941 aaatactgct ttatatcctt tagcagcaca tacaaatgct aaaccgatac ctgtattacc
     6001 acttgttggt tctacaattg tatcgccagg tttaattttg ccttctcgct ctgctttttc
     6061 aatcattgct aaagcaattc tatcctttac agaaccacct ggattttgat attccaattt
     6121 tacataaaca tctgctgcat tgtcatctac tacatttctc aatttgacta ccggtgtacc
     6181 gccaataatt tgagtaatat tatctactgg tttttgtgcc attaagaaca ctccttttta
     6241 gttagttgtt gaaatcttag tttatttatc ggatatagaa ttattttatc aaataaattc
     6301 tagaaagcaa acaaaatcac taaggattat acaactattt agttctacat ttcactaaac
     6361 cgctaaaaat aaacatttta gcgtattgat ttaaattaaa ttacgctaaa ctttctagca
     6421 acacgtttaa ttcttcttca gtatatttat atttatttcc acagaaatga catactgctt
     6481 cagcaccatg atcttcttta atcatatttt gaatctcagc ctcgcccaat cctttaatag
     6541 catttaaaaa tttctcatga ctacaattac attcaaattg aacaggcatt ttctctaaaa
     6601 tttgcacatg gtcttcacct aagatttcgt ttagtaatcc ttctggcgtt aatccttgtt
     6661 caattaattt agaaactggt gtcatttcac taattgcttt ttctaatttt gaaattgttt
     6721 catctttggc acctggcata acttgaatga taaatcctcc tgctgcttta atcgtattat
     6781 caggatttac caatacacca agacctaccg atgaaggtgt ttgttcactt gtagcataat
     6841 aataagtaaa atcttcacca agttctcctg aaacaattgg acttgctcca gagaaatagt
     6901 ctttcattcc aacgtcttta acaaccataa tagatccatt tgtccctact gctcgtctta
     6961 catcaagttt accttgctca tttaatggaa aatgagtttg tggatggtct acataagcac
     7021 gcacctcgcc tttagcattt gcatcggcaa taattcgtcc aataggtcct tggccatcta
     7081 cagtgacagt taatttttga tcacctttca acattgcgcc catcatagct gttgctgtca
     7141 ttgttcttcc cattgcagca gatgctgtcg gccatgtata atgtctcgtt tgtgcttctt
     7201 gaacagtttc agttgtcaaa gcagcataag ccctaatctc tccatcaaat gctaatgctt
     7261 taacaatata atcgtgtgtc attatttcaa tctcctctat tactctatat ttaaaaaatt
     7321 actttacttc ataaaatgca acaattgtac ttattctaca cccatcattc taaataatga
     7381 agtaacttgt tttacaatta ttttctgcta taacaattca acgacttaaa atctaatacg
     7441 tattttcaaa aacgataaaa gtacctcttt ctataacttt atcatagaaa gaggtactga
     7501 atataatcga ttatttattg tctgggtgat ttggatcgta aggtttttcg atatttgggg
     7561 cttgtcgatg tgctggttca tctttttcat cagatttatc agcttctttt ttatcctcag
     7621 caatatcttt ttcttcttta cgatcttcac tttcgtcacg ttgtccatct tctaattgct
     7681 ctttacgaat ctcttcataa gatttaccga atttaccatc attaaattca gaatcttcat
     7741 ctttaacaac tttagctgca tcataatcaa tttcaggtaa tttaccttcg tagaataatg
     7801 attgaatttg ttcagcaact aatgtttctt ctgttaataa tgtttcagca attaaaatta
     7861 attgttcttt gtgctctaat aaaatttgtt tacaacgttc gtattgttct ttaacgattc
     7921 gttgaacttc tttatcaatt tcatatgcga tttggcttga ataattaggc tcaccttgca
     7981 tatctttacc taagaatact tgaccattgc tatgaccgaa ctgtaatggt cctaattttt
     8041 tactcatacc atattgcgta accattgagc gtgcgatttg tgttgcacgt tcgaagtcat
     8101 ttgaagcacc tgttgatact tcgttaaagt taatatcttc tgatacacgt ccaccaagta
     8161 aaccacagat tttatctaat aactcttgtt cagtcattaa gaaacgatct tgtttaggta
     8221 gcatcattgc ataaccacct gcttgtccac gtggaacaat agtaacttta tgcactactt
     8281 ctgcctcatc aagtaccata ccgataattg tatgaccagc ttcgtgatga gcaacaatat
     8341 tacgttcttt cttagaaata actcgagatt tcttagcagg tccggctata actctatccg
     8401 ttgcctcttc gatatctctc atgtcaattt tctttttacc ttcacgtaca gcaattaaag
     8461 atgcttcatt taataagttc tctaaatcag cacctgagaa accaggtgta cgttgtgaaa
     8521 ttgcttttaa atcaaccgtt tcatcaagtg gtttgttttt agcatgaaca tgaagaattg
     8581 cttcacggcc tttcacatct ggacgaccaa cttgaatttg tctatcaaaa cgacctggac
     8641 gtaataaggc tgggtcaagg atatcaggac ggtttgtagc agctatcata atgatacctt
     8701 cattttcacc gaaaccatcc atttcaacta ataattggtt tagggtttgt tcacgttcat
     8761 catgaccgcc accaacacct gcaccacgtt gacgaccaac agcatcaatc tcatcgataa
     8821 agatgataca aggcgcgttt ttcttagcat tatcgaataa gtcacgaaca cggctcgcac
     8881 caacaccaac aaacatctct acaaagtctg aaccactaat agagaagaat ggtgcgccag
     8941 cttcacctgc aaccgctcta gcaagtaatg ttttaccagt acctggaggt ccaacaagta
     9001 agacaccttt aggaatccta gatcccattt ctttgaattt tttattatct ttcaagaaat
     9061 caacaatttc aattaattct tgtttttctt catctgcccc tgctacatca gagaaacgaa
     9121 cacgacgttt attattatcg tacatttttg ctttagattt accaaagttc atcatacgac
     9181 cgccactacc gccaccttgt gcttggctta ggaagaaaat aaataataac gctatgacta
     9241 caactggaat taatgttgaa agtatactca caaagacact ttgtttttct tcttctttaa
     9301 tcgttaattt tacaccgttt tgctttttag cagcatcagt aattttttgt aattcttttt
     9361 cgttgttata taaaatagtt gatgaatagt cttcatcatt tttcgtttta ccacttacca
     9421 tatagacatt ttgttgtggt tggatttcta aagttttaag gtcacctttt tccaacttct
     9481 cagtaaattg attatatgta agctgtttcg gcatatttcc attaccgttt aaatatgaaa
     9541 atagaccaaa aataataacg cctattatta cgataactag cacattgcga aaagctttct
     9601 gcatgcgtca tttcctccta cttcccataa taaaactaac aaaaattgta acatatgaca
     9661 tgttttataa gctagtaatt gagcttaaca tcaatgtcat tcacacccgt attagtttta
     9721 tcaatctcta tatttttcaa cttttc
//
LOCUS       DBNENW010000097         9679 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNENW010000097 DBNENW010000000
VERSION     DBNENW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.098"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(35..109)
                     /locus_tag="AC1LW8_001876"
     tRNA            complement(35..109)
                     /locus_tag="AC1LW8_001876"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Glu,seq:ttc)
     gene            complement(539..1822)
                     /locus_tag="AC1LW8_001877"
     CDS             complement(539..1822)
                     /locus_tag="AC1LW8_001877"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIP4773691.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2101..3501)
                     /gene="dnaB"
                     /locus_tag="AC1LW8_001878"
     CDS             complement(2101..3501)
                     /gene="dnaB"
                     /locus_tag="AC1LW8_001878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIP4773692.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3533..3979)
                     /gene="rplI"
                     /locus_tag="AC1LW8_001879"
     CDS             complement(3533..3979)
                     /gene="rplI"
                     /locus_tag="AC1LW8_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIP4773693.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3976..5943)
                     /gene="gdpP"
                     /locus_tag="AC1LW8_001880"
     CDS             complement(3976..5943)
                     /gene="gdpP"
                     /locus_tag="AC1LW8_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HIP4773694.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5958..6896)
                     /locus_tag="AC1LW8_001881"
     CDS             complement(5958..6896)
                     /locus_tag="AC1LW8_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HIP4773695.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7189..8157)
                     /gene="metX"
                     /locus_tag="AC1LW8_001882"
     CDS             complement(7189..8157)
                     /gene="metX"
                     /locus_tag="AC1LW8_001882"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetX"
                     /protein_id="HIP4773696.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8217..8315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8519..8848)
                     /locus_tag="AC1LW8_001883"
     CDS             complement(8519..8848)
                     /locus_tag="AC1LW8_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIP4773697.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(8845..9540)
                     /locus_tag="AC1LW8_001884"
     CDS             complement(8845..9540)
                     /locus_tag="AC1LW8_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIP4773698.1"
                     /translation="MTTHLSFRQGVRECVPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
BASE COUNT         3019 a         1910 c         1474 g         3276 t
ORIGIN      
        1 gcgtgttaac cgctacacta cgagacctaa aaaatggtga ctcctacggg actcgaaccc
       61 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caaggagccg ttcttaagca
      121 caagatagat tatagcacag ccaatcttgc catgacaaga tattttttac ataattttgc
      181 tattattttt tgaagaatgt tataagtgaa aaattgcatt ttattggcag tctatttatt
      241 ataataatta cgctattttt gaaatttttc tattttaggt gctttcattt acacttacgt
      301 ctactctatt ttaatagcta ttttcgacat cagacttctt acattcaagt atttcatctt
      361 aattttcgtt aaatatcatc taatcttcta aattatgact agcaaatatc gcctttacgt
      421 tagtattttt tatctaaata atcgaatcga tacagaagca agcataacaa aagcctccta
      481 ccaataaaga tagaaggctc atgtatttat aatcattgtg tatgacttat ataaagttct
      541 accacaattc ttttaatagg tttgtttgtt ctctatctgg accaactgag aagatagaaa
      601 tttgtacatt acataattct gaaatacgct ctaaatattt acgtgcattt tcaggtaatt
      661 cttctaaagt acgcacattt gttacgtctt ctgtccaacc tggtaactct tcaaagattg
      721 gtttacaacg ttttaattga tctaagtttg ctgggtactc agtaatttct ttaccgtcta
      781 attcataagc tgtacagatt ttcactgtgt ctaggcctgt taaaacatcg attgagttaa
      841 tagataaatc tgtaatacca cttacacgac gagagtgacg taatacaact gaatcaaacc
      901 aacctacacg acgtggacgt cctgttgttg taccgtattc acgaccaacc tctctaatat
      961 gatgtccatc ttcatcgaat aattcagtag ggaatggacc atcaccaaca cgtgatgtat
     1021 aagctttaca tacaccaatt acctttgaaa cgaatgtagg acctacacct gtaccaacag
     1081 taacgttacc tgcaattgga ttacttgatg taacgaatgg atatgtacca tggtcgatat
     1141 ctaacattac accttgcgca ccttcgaaaa gtaccttttc atctgctaca aatgcatcgt
     1201 ctaagatttt tgatgtgtct gttacaaatt cttttaaacg ttgacctgca gcatagtatt
     1261 cttcaaagat atcatcaaat gatggacatg tttcgttaaa catacctttg aaatatgctt
     1321 gtttatattc aatgtttgat tttaataatc tttcgaatgt ttctttttca agtaaatctg
     1381 ccatacgaat accgatacgt tgaactttgt ctacatatgc tggaccgata ccttttttag
     1441 ttgtaccaat cttattgtca ccgcgtaaac gttcttcata ttcatcttgt gctaagtgat
     1501 atggtaaaat cacttgcgca cgattagata tacgtaaatt acttgtagga atgccacgtt
     1561 catttaatcc gtctaattct ttcaatagtg caactggatc aacaacgaca ccgttaccga
     1621 ttaccgctaa tttgtctttg taaaagatac cagatggtac taaatgtaat ttatatgttt
     1681 ctccgccaaa ttgaatggta tggcctgcat tattaccacc tgaaaaacgc gcgataacat
     1741 ctgactgttc tgccaagaaa tccgttattt ttccttttcc ttcgtctccc cattgtgtcc
     1801 caactactac gattgatgac atgtgagcac ctccaagttt ttctcattta accatttgat
     1861 attctaccaa tatcgagacc ataatgcaaa taaaaatcga acattgattt tcagcctttt
     1921 taaaagaaaa atcattttaa caaatcgtct tcaacgtcct aaaaaccctt taacagaggc
     1981 gttgacagta ctaatccatc actttcaata cacaaactta acatatatca acccgttcag
     2041 aacaaaataa aaaccgtaca attttatcat cgcaatgatt attgtacgga aaaacttttt
     2101 ttacatcata tcagcatgtg cataatcgat atctgtaaat ttattatatt gtttcataaa
     2161 gtgtaactta actgtgcctg ttggaccgtt acgttgctta gcaatgatga tttctatttc
     2221 accgttttca tcattcgttt gtggctcaaa accaccatca tcgtcatcat cttcatcgcc
     2281 gccacggtta tagtaatcat cacggtataa gaacgcaacg atatcagcat cttgctcaat
     2341 cgaaccagat tcacgaatat cactcatcat tggacgttta tcttgtcgtt gttcaacacc
     2401 acgagataac tgacttaatg cgataactgg acattctaat tcacgggcta atgcttttaa
     2461 tgtacgagag atttcagaaa cttcctgttg tctgttatcg gacgcacgtg aaccactacc
     2521 ttgaatcaac tgtaagtagt caatcacaat catgtctaag ccatgttctt gctttaatcg
     2581 acgacattta gaacgtaaat cattaattcg aatacccggt gtatcatcaa taaaaatctt
     2641 cgtacgtgat aatttaccta ccgctatagt aaaacgactc caatcttcct cagtcatagt
     2701 acccgttctt aagcggtttg agtcaacatt tccagaacta caaatcatac gtgtggctaa
     2761 ctgatcagca cccatctcta gcgagaaaat accaactgta tacatatctt catgcgttgc
     2821 aactttttgt gcaatattaa gtgcgaacgc agtcttacct acagatggac gcgctgcaag
     2881 gataattaaa tcatttcggt tgaaccctgc tgtcatttgg tctaaatctc gatatcctgt
     2941 aggtatacct ggtgtttgac cactattttg atcaagctct tcagctgttt catacacttg
     3001 tcctaagacg tctcgaatgt ctttaaagcc atcgctttca cgagaagatg atagctctaa
     3061 aattcgacgt tctgcatcac ttaaaatcgc atctagttca agttcatcat tatatccatc
     3121 attggcaata ctatctgcag tttgaatcaa tctacgtttt aatgcatgct tagaaacgat
     3181 atcagtataa tactgaacat ttcgcgtcgt tggtacattt gtagataact ctgcaagata
     3241 ttgcgggcca cccgcttcat tcaacgtacc ttccgtcgat aattgatcca tcaatgttac
     3301 aacatcaatt tctttattat cttcatttaa gtgcatcatt gcacggaaaa tatgttgatg
     3361 ggcaccccta taaaacgact caggaagcaa aacttcctga gtagtattaa tcaattctgg
     3421 atctataata attgaaccta agacagactg ttcagcttca ttgttatgcg gcatttgatt
     3481 ttgctcatac attctatcca tgaatggtta cacctcttat ttcaatccaa ctttattgtt
     3541 caactgtgtg tacgcgaatt gtaccttcaa cttctttatc taatttaaca ggtacattcg
     3601 tatatcctag ggaatgaatt ccatttggta aatccatttt acgtttatca attttaatat
     3661 catgttgtgc ttttagtgct tcggcaattt gttttgtact tactgaccca aacaatttac
     3721 caccttcacc agtttttgct gatacttcaa cttcaatgtt tgataacgtt tcttttaatg
     3781 ctttagcatc ttcaatttct tgttggcgtt cttgttttgc acgttttttc tgtaactcta
     3841 attgtttaag gttacctggt gttgcttcta cagcataatt ctttttcaat aagaagttat
     3901 ttgcataacc tactggtact tctttaactt cacctttttt acctttacct ttaacatctt
     3961 gtgtaaaaat tactttcatg catcttcact cctacttaat tgttctgtaa ttgcttgttg
     4021 taattgtgct atcgcctctt cgactgtcac acctttaagt tgtgttgccg cattggttaa
     4081 atgtccaccg ccaccaagtg cttccattgt taactggaca tttactgaac cgagtgaacg
     4141 cgcagatata ccaatcagat tatcttcacg tctcgcaaca acatatgatg cttcaatacc
     4201 ttctaaactt aacagttcat ctgctgcttg tgcaactgtt actggatgat aaattttatc
     4261 gtctgaacca tgcgcaattg ctatgccatt atcttcaact tttacagttc gaattaattc
     4321 agatcgatta atgtaagtat ccacatcatc ttttaagaaa tgttgcgtta aaatcgtatc
     4381 tgcaccatgt gcacgtaaat aactcgctgc atcgaatgtt cttgatcctg ttcgtaatgt
     4441 aaagtttctt gtatctacaa taatacctgc atacatcact gttgattcaa gacgtgttaa
     4501 acgttgttct gttggttgat attccagtaa ctctgttacc aattcagctg tcgaacttgc
     4561 gtatggttcc atatatatca acaatggatt agagatgaag ctttcaccac gtctatgatg
     4621 atcgataaca actttacggt ttgctttatt taagacattt tcatctaaaa ccagttccgg
     4681 tttatgcgta tcaacaatca ctacggttgt cttagatgtc atcatatccc aagcatcatc
     4741 tgatgtaata aatcgctctc ttaactctgg ctttttatca atttcgttca tcacgcgtcg
     4801 taatgttgga tcaatgtcag tctcatttaa tacgatgtat gcttctaaat tattcatcat
     4861 tgcaaatcta gacacaccga ttgctgcacc aattgcatct aagtcaggac gtttatgtcc
     4921 catgataatg actttgtcac cctctgcaag gatatctttt aacgcatgtg agataacacg
     4981 cgctcttaca cgagtacgtt tctccatcgg gtcagtctta ccgccataga agcgcacatt
     5041 accattaata cttttaattg caacttggtc gccaccgcgt cctaatgcta agtctaggcc
     5101 tgattgtgat aattcaccta aatcgattaa attttcagta ccttcaccaa caccaatact
     5161 taatgttaat tgggcacgat aaccaacact tttttcacgt aattgactca agatatcaaa
     5221 tttagattct tctaagtcag ctaatatttt ttgatttaaa taggctacga attgatcgga
     5281 actatacctt ttaaagaata tattatactc agttgcccat cgactaataa cacgcgttac
     5341 cattgaattg atttccgaac gctgcgtatc attcatattt tgagtaatct catcgtagtt
     5401 atctaaaaat aatgtcgcaa tgattggttt agaattttca tatagttcat ttgtttgtac
     5461 ttgttcagtt atatcaaaga aatagaggca gtgatcattc tcagaataac gtacttggaa
     5521 atgatactga ttatattcta tttcaacgga tttcactcta tctaattgct ttaaaatgtt
     5581 tggaaatact tcatttacag attcagaaat gacatttgct tccatatgat ctgtcataaa
     5641 ttggttaacc cattcgatgt gatcattttc atctaaaaca atgataccaa ttggtaaatg
     5701 tttgattgct ttattatttg ttgttgaaat ttgagcactc aaaccatcta cataactatc
     5761 cattttcatt aaagcttgtc tgaataaaac gatgctaaca ataatcatca cgacaagaac
     5821 gatagatgca attagtgcta taagactatt aaagataaac catacaccca ttaaaacaat
     5881 tgctgtgatg atcatgatga caaatggtat tagtaaagct ttcttagtgg actgccgatt
     5941 cattattcca cctctattca ctttttagaa ttatttttca tgattcgctt caaattcaaa
     6001 cttaaatcga taacaccaag tagtcctaca atatgtgtcg taggtgtcag tattgtaccg
     6061 ataatcaata gtaaaatcgt tactgcattc ggcaaacctt tcgctttacc aaagaaatga
     6121 ataacactta aaccttgaat atacattact aatgataaca caagttggaa gtttaaaaga
     6181 atgctctgga acacactcgg ttgacctgta aataataaac atatgataac aataatgtat
     6241 atccataata aaataccgct catttgccac gcgaaaagtg gcttaaatac aggtgtagcg
     6301 attttaaatt ttcgtaaaat cggaaatgta acgattaagt taattaagac gattaaaaat
     6361 gtaatgataa tgatgaaacc tggtaattga acggtcgctt gtctaaaccc ttcttctaat
     6421 atttgggtca tattcgcatc ggcaccgctc atcgtaatcg cttcatgtaa tgtttgcttg
     6481 aaaggtttta ctatgctcgc tgatggtgga attcttccga atgtttgtag taacataaaa
     6541 gcgattaatg aaattaagct catcgctact gttgttacgt ataatattct ttctttagac
     6601 gttctttctt tgagcaattg accaataatt aaacttgcaa ttaagactaa tatgatggca
     6661 cttaaaacga aagtattacc taaaacagtt gttataatta ctgtaataag tgcactaatc
     6721 ccgaaagatt gtattgattt attccataaa acgatacctg gtatggttgc aaacaatgca
     6781 aatattagtc ctaaaccagg cactagatat aaagctaaag cgacaaatac caacgcaatc
     6841 gttgcaatta ttgttgcttt aggttgtatt tttgaaaaca cataagccac tcccatattt
     6901 ttaactatag ctattatttt aacctcttta atggaattta acaatttata gattgtatgc
     6961 ttctatttca tttaattgaa taataacttt catgttttat aagtaattaa catactcatt
     7021 tgaatcgctt ttgtgtgctt tcattttcaa catgattatt taatcccact acatagcaat
     7081 caagcttgat ttagatttac aattcattac cactctcatg tactctagat gtttttgaat
     7141 atgatagctg tgatttagtg gcttcattct ttgaaaatat atatattatt acttacgctt
     7201 aaaatgcttt aaatttaaga aatgatataa gttaggtgcc caggtactaa agtttagtag
     7261 gaatccatca tgcccaacat tatcaggcac gaagaaatga cgatgatatt taaaacgttc
     7321 acctaatgca cgaacttgat catccggata tagcaaatca tctatgaacc ccatcgttaa
     7381 cacttttgtt tctaaatttt taaaaacatg cgttacgtct gtgcgacctc ggtcaatgtt
     7441 gtgactatcc aatacatcta gcagtgtcag ataacaattc aaatcaaaat gttctttaaa
     7501 tttattacct tgatgttgtt ggtatgcgac tacttcatcc ggcgtgaaac gttcatcata
     7561 actttttgat gatcgatatg tcaaaaaacc taattggcgt gcaatactta gaccttcctt
     7621 accaccaaga tgaatggctt gccttgcaat ttcattgaaa gctctactat aagatgatgt
     7681 tcggcttgtt gcagcaagga taatggcttt atctacttca aactgttgat tgtaaagtag
     7741 ttccattgct tgcattcctc caaggcttcc ccctattaaa atattaatct tgtcataacc
     7801 aagggcttgt atacctcgtt cattcgctct gactatatct cttaatgtta attttttagg
     7861 aaaatgaggg tcgtttaaag gtgaacttga accgaaagga ctaccaataa catcaaatgt
     7921 taaaaattga taatcgtgaa tgggtatata tcccccatca ataatttctc gccaccaacc
     7981 cggataatca tctgttccat atgttaaatg attgccagtt aatgcatgac aaactacaac
     8041 taatggttgt ccatgataac cgacatgctc atatctcaaa cgcaagttat ctatgacttc
     8101 cccagattct gtaataaatt tccctaaatt taaagtatct actgtgtaat ttgtcattgt
     8161 tttttcctcc ttaaacaaaa aaacttctca ccctattgaa aagtaagaag tctttatact
     8221 tatcattcga gtaactcgtt ggttttagca ccgtgctata aagtcggttg ctgaagtatc
     8281 acagggccaa gtccctcaac ttctcacaat aagaaatatg aaattgttat gtgttagttg
     8341 agattcagtg atgaattact ttatcattta aaatgttgtt atcattgtca tgcgttagcc
     8401 aaatcgctta cgtatacacg attcccaatc ttaacataga cgatttgtat atcagaattt
     8461 tctgattact aacagtttac ctaagtttaa atatctgttc aatgattttc agttattttt
     8521 aaaagaaaaa tcgtaatgct gccatgataa caatcccact aataattgta atagttaaac
     8581 tacgcgtgat tatagataaa ataaccgtcg gaatgagcgc gataatgtaa gggatgttta
     8641 atgtataccc ctcaccatga ggcgtctgtt gaataatgct gtctatgaca agtgccgtaa
     8701 atagtgtgat tgggataaat gatagccatc gaaccacgac atcaggcaat tgcacttttg
     8761 aaatcatgat aaaaggtata attcgaatta atagcgttac gataccacac aataaaataa
     8821 gtattaacat gttcatatga gttatcatcg ttccatcatc actcctaacg ctgctgaaat
     8881 tgtggctgca attaatattg ctagatatga aggcataaac atacttagcg ataacatcat
     8941 tactatgacg gcaataatga gtactatgta aattcttaat cgcgatttag taattgattc
     9001 aaattgcgca atggccaaaa agataaacat agccgtgata gcaaaatcta accctaatgc
     9061 ttgtggattc gaaatatact cgccaaacaa tgcacctgct acacatgaaa ttgcccaaaa
     9121 taaatatgct gtgatattaa gaccatgcat ccaacgatca ttgatagctt ctccttttaa
     9181 ataaggtgta atggcgacgc caaacgtttc gtcagttact aatgaaccta atccaacacg
     9241 gttccaaaac ccatatgtct tgaagtttgg tgcaagcgac atacttaaaa ggaacattct
     9301 tgaatttacg ataaatacag ttagtacaat cgctgatatc ggtgtacctg ctataaacaa
     9361 cgcgcacata ataaattgcg cagcaccggc atatataaca agacataaca agacaatttc
     9421 taaaatacta aagttttgag acgaagccac aataccaaat gaaataccaa caccggcata
     9481 acccaataat gttgggacac actctcgcac gccttgtcta aaacttaaat gtgttgtcat
     9541 ctcaactacc tcctttgcca ctttatattt atataattac gaaaaaaata atatacctag
     9601 tctaatacag ttctatatca caagtaataa cgttagacac tggtttgtct gaagttagac
     9661 ataccggagt ctttaatca
//
LOCUS       DBNENW010000098         9602 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNENW010000098 DBNENW010000000
VERSION     DBNENW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.099"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            70..705
                     /locus_tag="AC1LW8_001885"
     CDS             70..705
                     /locus_tag="AC1LW8_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIP4773699.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1009..2088
                     /locus_tag="AC1LW8_001886"
     CDS             1009..2088
                     /locus_tag="AC1LW8_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIP4773700.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILKIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            2148..2372
                     /locus_tag="AC1LW8_001887"
     CDS             2148..2372
                     /locus_tag="AC1LW8_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIP4773701.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2580..3830
                     /locus_tag="AC1LW8_001888"
     CDS             2580..3830
                     /locus_tag="AC1LW8_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIP4773702.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDKIAMLAFTNTVISASAGSIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAALTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4013..4963
                     /locus_tag="AC1LW8_001889"
     CDS             4013..4963
                     /locus_tag="AC1LW8_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP4773703.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANALENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5112..6596
                     /locus_tag="AC1LW8_001890"
     CDS             5112..6596
                     /locus_tag="AC1LW8_001890"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HIP4773704.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYSEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6593..7552
                     /locus_tag="AC1LW8_001891"
     CDS             6593..7552
                     /locus_tag="AC1LW8_001891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIP4773705.1"
                     /translation="MRRLFLIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7740..7849)
                     /locus_tag="AC1LW8_001892"
                     /pseudo
     CDS             complement(7740..7849)
                     /locus_tag="AC1LW8_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7921..8637)
                     /gene="agrA"
                     /locus_tag="AC1LW8_001893"
     CDS             complement(7921..8637)
                     /gene="agrA"
                     /locus_tag="AC1LW8_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing response regulator AgrA"
                     /protein_id="HIP4773706.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8656..>9602)
                     /gene="agrC"
                     /locus_tag="AC1LW8_001894"
     CDS             complement(8656..>9602)
                     /gene="agrC"
                     /locus_tag="AC1LW8_001894"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quorum-sensing sensor histidine kinase AgrC"
                     /protein_id="HIP4773707.1"
                     /translation="FLVVYVSISYALAYILNRILKRINGTYLSLNKKFLTVITIVIVI
                     TFSLLFAYSQIDASDASTIKQYSLLFLGIIILLSILIFIYSQFTLKEMKYKRNQEEIE
                     TYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMTGLRDYFNKNIVPMKDNLQ
                     MNALKLNGIENLKVREIKGLLTAKILRVQEMNIPISIEIPDEVTRINLNMIDLSRSIG
                     IILDNAIEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEG
                     RGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3345 a         1329 c         1774 g         3154 t
ORIGIN      
        1 aacaattacg ctctaactcg tttgaataca tagatataat tcattcaaac acgaaggagg
       61 aaatagcgaa tgagtgacca agttaaaatt cctcgagcaa ctttaaaacg tttgccgtta
      121 tattatagat ttgtcagttc attaaaatct aaaggtatag atcgtgtaaa ttcaaaagcg
      181 attagcgatg cgttacaaat tgactcggca acaattcgtc gtgacttttc atattttggc
      241 gaattaggta aaaaagggta cggatataat atagatagtt tattggattt ctttaaatct
      301 gaactaagcg agagtgacat gatcaaaatc gcaattgtcg gagttgggaa cctagggaaa
      361 gctttgctca catataactt ttcaatacat gacgatatga cgattacaga agcgtttgac
      421 gtaaaagaag atgttattgg ccagaaaata gggaacgtta ttgttaaaga taacgatgaa
      481 ttaataacaa cattgaagaa ggaagaaata gatgttgtga ttctaactac accagaaaga
      541 gttgcacaga aagttgcaga tgaactcgtc caagctggtg tgaaaggtat tttaaacttc
      601 actcctggta gaattaatac gccttcagat gtgcaagtac atcaaattga cttaggtata
      661 gaattacagt cattattatt ctttatgaaa aattacagtg aataaatgtg atgttagctt
      721 tgaatgatta accttgttat atctttaatt aattatagaa cagtctggga ctaagtatgt
      781 ctcaggcttt ttctgttatg tttgaaaatg aaaggaaaaa ctgttgtgct gataaatata
      841 gtataggtta ataaattaac acgtagagat ataacgatga atcgtaatta atcgccttcc
      901 ttatatgaat ataatataaa tatcaaaaat attgtgtata aggttgaaaa caagaaaatc
      961 atcacctata atacatacta aacttatcgg aaatgagagg gtaggattat gtggatgatt
     1021 tttagtggct taattgtcgg gggactctta ggttttgtca tgcaaagaac aagattttgt
     1081 ttaacaggtg gctttcgaga tatgtatgtg caaaagaata ataagatgtt ctatgcatta
     1141 ttaatcgcta ttactattca aagtatagga ttattgattt tgacggcaac agatatttta
     1201 aaaattcctg cacatagttt tccaatattg ggaacaatta taggttcttt tatttttgga
     1261 attggaatag tattggctgg agggtgtgca acaggtactt ggtatcgcgc tggtgaaggg
     1321 ctaattggta gttggattgc attagtatta tatgctgtta ctgcagcaat cactaaaaca
     1381 gggattttaa agccagtaat ggataaaatt aatcaaccaa cgaatgtaaa tagtgatatg
     1441 tctcaaacaa ctggcattcc gttttgggga ttagtcgtta tattaactat aatcaccatt
     1501 tttctagttg taagaacact caataataag aaagttcgag ttgctgttcc aaaactaaag
     1561 caacgttata caggtattag atattacctt ttcgaaaaac gataccatcc atttattgca
     1621 gcaattgtaa ttggacttat cgcactctta gcttggccaa tgagtgcatc aactggaaga
     1681 aatgacggtt taggtataac aacgccatca gcaaatttag tacacttttt gattacaggt
     1741 gaaactaaat ttattgattg gggtgtcttt ttagttctag gaattttcat tggttcatat
     1801 attgcagcta gaggatcaag agaatttaaa tggcgattgc cagataagat tacaatacga
     1861 aacagtgcta ttggtggcat atgtatggga tttggtgcgt cagttgctgg tggttgttct
     1921 atcggtaacg gtttggttga aacagcaacg atgacttggc aaggatggat tgcgctagca
     1981 tcaatgatag ttggtgtatg gacaatgagt cattttatct ttgttcgtcc aatgaaaaaa
     2041 gtacaacaac aatctgcaaa ggttaaacag caaacgcaaa tagtatagaa gattattatg
     2101 caaatgatgt tgatcaaata aaagtgattg gaaaaggaga aataattatg atacacgaat
     2161 taggtacagt aggaatggta tgtccatttc cgttaattga agcgcaaaag aaaatggcaa
     2221 cattgcaatc tggagatgaa ttaaaaattg attttgattg cacgcaagcg acggaagcta
     2281 ttccaaattg ggcagcagaa aatggatatc ctgtaacaaa ctatgaacaa attgataatg
     2341 cttcatggac aattacaatt caaaaagttt aacgttatca ttttaacaat aaaatagatg
     2401 ttagattcta tggctacttc cgctaattta aaagtgagta agtagtcttt tttgttttag
     2461 ttcatgaaat cacttttata tagtgtggca cattttatcc caaaagatgt aataaaactt
     2521 aacgcatttt tgctttttat aaattgtcag attattatga aaacaaggga gtggtaagta
     2581 tgaatcttaa cgatacgata tttatgtttt tgtgtacatt attagtttgg ctaatgacac
     2641 caggattaag tttattttat ggtgggttag ttcaatctaa aaatgcgctt aatactgtca
     2701 tgcaaagtat ggcagcaatt gtgcttgtta catttgtatg gataacagtt ggttttacaa
     2761 ttagttttgg gaatgggaat ttatggttcg gaaattggga atatactttt cttaatcatg
     2821 taggttttgc gactcaagaa gatattagcc cacatattcc tttcgctttg tttatgttat
     2881 ttcaaatgat gttttgtacg attgcaattt ctattttatc tggttcaatc gctgagaaaa
     2941 tgaagtttat tccttattta ttattcgtag taatatggac tgctcttgta tacagtccag
     3001 tagcacattg ggtttggggc ggcggttgga ttaacaaact cggtgtatta gatttcgctg
     3061 gaggtacggt tgttcatatt acatcaggtg tttctggttt agtattagct attatgattg
     3121 gaaaaggaaa caaacattct gaatcaacac cacataatct tatcattacg ttgattggcg
     3181 gtatattcgt gtggattggt tggtatggat ttaatgtagg tagtgctttt acatttgata
     3241 aaattgcgat gcttgcattt acaaatactg tcatttcagc cagtgcaggt tctataggtt
     3301 ggttaatttt agaatatatt tttaaaaaga cgacaagttt acttggactt ttactcggtg
     3361 cattagcagg attagttgtc attactcctg cagcaggata tgtaacatat cttagtgcaa
     3421 caataatggc tttaatagga ggtatctgtt gttatattgt cattaattac atcaaggtaa
     3481 aactaaaata tcatgatgca ttagatgcat ttggtatcca cggtgttggt ggtattattg
     3541 gtgctgcttt aacagcagtt ttccaaagta aaaaagcaaa tcctgacatt gaaaatggtt
     3601 ttatttatac tggtgacata catattatac ttgtacaaat attatgtgta acagcagttg
     3661 taatttttag tatcgtcatg acgtttatta ttgcgaaagt aattaaatta attacaccat
     3721 tatctgttac ggaacaagaa acgaatatag gattagacaa gattgttcac ggtgaacatg
     3781 cttactttga aggtgagcta aatagattca ataaacatat tcgatattag aatatattta
     3841 catagaatat tcattgtcct gacatttaac taaaggttga tgttgggaca ttttgttata
     3901 caaaagtttt attttgaaat ctttttatga aagaagcaga aatattattt aaagcggtta
     3961 cacatatgct aaaataaggc taagtgtcac aaataatgat aggtgaataa gtatgaaaaa
     4021 tatatctgat attgccaaat tggcaggcgt ttcaaaaagt acagtatcta gatttttaaa
     4081 taatggatct gtcagtaaaa aaacaagtga aaaattaaca agaattatag cagaacatga
     4141 ctatcaaccg aatcaatttg ctcaaagttt aagagcgaga caaacacacc ttattggtgc
     4201 cattatacct agaatgaatt catatgcagt agacgaaaca atcaaaggat tggcaaaaca
     4261 atgccaaaaa tatgaatcac aattaatttt aaattataca ggtttaaata tcgaagcaga
     4321 aatacaagcg cttgaaacat tagcacgcag taaagtagat ggtattgttt taatggctac
     4381 agatataaca gagagacata ttgaagtcat taataaaatg aatgtaccaa tcgttattgt
     4441 tggtcaacaa catgaacaac ttcatagtat tgtgcatgat gattataaag caggtcaaat
     4501 tataggcgaa tggattggtc aacagggata tcaacaagtt gaagtgttta gcgtaagtga
     4561 aaaagatatt gcagttggta tacatagaaa acgtggttta cttgaccagt tagctaaata
     4621 ccaaattaaa cctaatatac atgaaacaaa ttttacttat gtggaagcac aaaaagatgt
     4681 tgcaaatgct ttggaaaatg tggagcaagt agatgcggtt gttggagcaa ctgatacgat
     4741 tgcattagct gcctataaat attattctga taaaaaagat gttatgaaac cacatcaaat
     4801 atatggtttt ggtggtgacc caatgacaca attagtgtct ccatcgataa aaacaattca
     4861 ttataattat tttgaagctg gccaatgcgc gatggaagag atacaacaga tgcttaaaaa
     4921 gcaagatatg ccatatagcg tcacagtaga tgttaatatt tagacgctgt attttttaaa
     4981 ataaatgtgg aaccgatacc atataactat aaatggatag gttaaaagtt aaagaacgta
     5041 ggtaaaattt gctataatag aatataaatt gttaacagca taaattataa aaggaggact
     5101 gggtaaatat tatgaccgaa tggactagag aagaacgtta tcaacgaatc gaggacgttg
     5161 atactgagta ttttaaaaca ttaaaacaac aagttgatca atcaaaattt cgtcaacaat
     5221 ttcatataca accagaaaca ggcttattaa atgaccccaa cggactaatt ttttataaag
     5281 ggaagtatta tgtttcacat caatggttcc cattaggcgc agtacatggc ttaaagtatt
     5341 ggtataacta cacgagtgat gacttaataa actttaaagc tgaagggcca attttaaatc
     5401 cagatactaa atatgacagc catggtgtat atagcggtag cgcttttgaa tataacgggc
     5461 atttatatta tatgtacaca ggaaatcatc gagataatca ttggcaacga catgcgagtc
     5521 agatgatcgc acgattgaaa gaagacggtt cagttgaaaa gtttccaaag ccagtaatta
     5581 gccaacaacc agaaggatat acgagtcatt ttagagatcc taaagttttt aaatatagtg
     5641 agaaatatta tgcgatcatt ggcgcacaaa ataatgatca gcaaggtcga ttattacttt
     5701 ataatactga agatataatt aattggcatt atttaggtga aataaataca gagttggatg
     5761 attttggata tatgtgggaa tgcccagatt actttaatgt agataatcaa gatgtcatac
     5821 ttatttgtcc acaaggtatt gaaccaaaag gcgatcagtt caaaaatatt tatcaaagtg
     5881 gttatatact tggaaagttt gatattgaaa agttaacata tgaacatgaa aattttgtcg
     5941 agcttgataa tggctttgat ttctatgcac ctcaaacatt tttagatgaa aaaggccgac
     6001 gagtactaat tggatggatg gggttaccgg aaatcgaata tcctactgat aatgaaggat
     6061 gggcccattg cttaacaatt cctcgtgtat taaatgtaga aaatggtcaa cttaagcagc
     6121 gtccatatcc agcgttggaa aaattacgtc acaataaaga gacagcatta ggctacgcaa
     6181 ataaatttac tcgaaaatta catccgtatg aaggtaaaca gtatgaatta atcatagata
     6241 ttttggataa tgatgctacc gaagtgtact ttgaattacg tacatctaag acttcttcaa
     6301 cattaatcgc ttataacaag cgtgaaaata aaataacatt agatcgcagc gacagtggtt
     6361 tattgccgac aaatgttgaa ggtacgacgc gtagtacgat attagacacg ccattaaagc
     6421 aattacaaat ttttgttgat acatctagta tagaaatttt ctgtaatgat ggggaacgtg
     6481 ttttgacatc tcgaattttc ccgacagaag acgcactagg tattaaaaca tcaacagaat
     6541 cagggcaagt atatttacaa tttactaaat atgatttaaa ggatgaacat aaatgagacg
     6601 tctattttta ataggagaag cgttaattga ttttattcca aatattacaa atgcgaattt
     6661 aaaagatgtt caaacattta cgaagcaaat tggtggcgcg ccatgcaatg tagcttgtac
     6721 agttcaaaag ttaggacaac aagcgtatat gattacacaa ttaggtaatg atgcatttgg
     6781 agatagcatt attgaaacga tatcaagtat tggtgtagat gtatcaaaag tgtatcgcac
     6841 gaatgaagca aacactgcgt tagcttttgt gagtttgact gaagcgggcg agcgagattt
     6901 ttctttttat cgtaagccct ctgctgatat gttatttgaa ccaagttttg tgaatgatat
     6961 tgatgtgaat gaaaatgatg tagtacattt ttgttctgtt gatttggtcg atagtccgat
     7021 gagagatgca cattatcaat tgattaccaa aacattaaat gcaaatggta ctgtcgtatt
     7081 tgatcctaat gtaagattgc cattatggga taacgctgaa gatttaagac aaactattca
     7141 tacctttttg ccattggcac atattgtaaa agtttcagat gaagaattag aatttattac
     7201 aggtattcat gatgaaaatg aagccattca atcattattt acaggtaatg tcactgtagt
     7261 catttatacg aagggagcag atggtgctgc cgtatatttg aagaatggta ttaatcatta
     7321 tcatagtggc tataaggtaa aacctgttga tacaactggg gcaggggatg cttttattgg
     7381 tgcagttatt agcaggattt tagcaaccga tgtgttaaat ttaactcagt tatttgaaaa
     7441 tgaaggtgag gaaattttag cgtttagcaa tcgcgttgct gcaatagtga caacgaagta
     7501 tggtgctatt aatagtttgc caacattaca agaggttgaa caagcatttt aaataattca
     7561 tttataaaac agtgtaggaa acaagttgaa tcttgtgcta cactgttttt taaaacaaaa
     7621 taagattcac ggggtaggaa attgtagcat tttatttatg gaaactgtca atttcaaatt
     7681 tagtatattt tgttaaaaat tgcgccatag gattgtagag tgaaatagaa attgataaat
     7741 tatgtagtaa taggaatagg tatacaaaga aacgttacat cattttttaa ttcacgcgtc
     7801 atatttaatt ttgttattaa acaattaaaa agccagctat acagtgcatt tgctagttat
     7861 cttgttaaaa tccaacaaga tttacaattg aatacgccgt taactgactt tattatctta
     7921 ttatattttt ttaacgtttc tcaccgatgc atagcagtgt tctttatttt taaaatagac
     7981 aattcgctct ttcgaatcta tagattcaat attatggcga ttgacgacaa agctattatg
     8041 acatctaaag aaacgatcat ctaattgact cagttctttt aaattaccat aaaattcaat
     8101 ttgacggtta tctaaatggg caatgagtct gtgagatttt gttgatgatt caaaaaacat
     8161 aatatcatca tattgaacat acactgaatt actgccacgt tttaattcaa tcgtttcaac
     8221 gctattatct ttagacaaca attgtaagcg tgtatgtgca gtttctaaac aatctataat
     8281 tcgagttctt aattctgctg gatcatcttt aaaaataaaa tccatcgctg caactttgta
     8341 gacaaatgtt aaataggtaa gttcactgtg actcgtaacg aaaataatgt taccaactgg
     8401 gtcatgctta cgaatttcac tgcctaattt gataccatta atatcagttg aaagttgaat
     8461 atctaaaaag taacagccta tgtcattcat atttttagct tgctcaagca cctcataagg
     8521 attatcagtt gcgagggcaa tttccatagg cttttcttct atcattatat aatttttaat
     8581 aatggtaacc atgttttctc tttgttttgg atcgtcttcg caaatgaaaa ttttcataca
     8641 ttcacatcct tatggctagt tgttaataat ttcaactttt tgaataaaga aaccattttc
     8701 gataattgta tctaataaga cattgtctgc attatcagca atttctttta aagttgatag
     8761 acctaaacca cgaccttcac ctttagtaga aaaactttct tggaacaatt catgaatacg
     8821 tggtatatca tctgcacatt tattcataac aataaacgtt actgaatttt cgctttcaat
     8881 aaatgcaact cgaatgatag gatcatcaat ttcacttgat gcctctattg cgttatcaag
     8941 aataatacca atactgcgac ttaaatcgat catattcaaa ttaatgcgag ttacttcatc
     9001 tggtatttct atactaatcg gtatattcat ttcttgtaca cgtaaaattt tagctgttag
     9061 aagaccttta atctcacgta ctttaagatt ctcaatacca tttaacttaa gagcattcat
     9121 ttgtaaatta tctttcatag gtacaatatt tttattgaaa tagtctcgaa gaccggtcat
     9181 gtcatcttca cgaatgtatt ctgaaagagt cgtcaagata ttgacataat catgacggaa
     9241 cttgcgcatt tcattattaa ttgcttcaat ctttaatgtg tattcataat aggtttcaat
     9301 ttcttcttga ttacgtttat atttcatttc tttaagagta aattgagaat aaataaaaat
     9361 taagatactc agtaatataa taattcccaa gaataaaagc gaatactgtt tgattgttga
     9421 tgcatcactc gcatcaattt gcgaatatgc aaataacaaa gagaatgtta ttactataac
     9481 aatagttata acagtaagga attttttatt aagagaaagg tatgtaccat ttatcctttt
     9541 taatattcta tttaaaatgt aggcaagtgc ataagaaatt gatacataca ctaccaaaaa
     9601 ga
//
LOCUS       DBNENW010000099         9526 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNENW010000099 DBNENW010000000
VERSION     DBNENW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.100"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(281..760)
                     /gene="rlmH"
                     /locus_tag="AC1LW8_001895"
     CDS             complement(281..760)
                     /gene="rlmH"
                     /locus_tag="AC1LW8_001895"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIP4773708.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVTDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1128..3446)
                     /gene="adsA"
                     /locus_tag="AC1LW8_001896"
     CDS             complement(1128..3446)
                     /gene="adsA"
                     /locus_tag="AC1LW8_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HIP4773709.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDRQQVPPTKEAAHHYGEEAATNVSASAQGTADDTNNKVTSNAPTNKPTTAVSTTG
                     NKRHDVDAQQASTQKPTHTATFKLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALVQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFESIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDMTEPQRMLLGKPVASEQPAKGQQGSKGSESGKDTQPIGKEKVMNPA
                     KQPATGKVVLLPAHRGTVSSGTEGSDRALEGTAVSSKSGKQLANMSAPKGSAHEKQLP
                     KTGTDQSSSPAAMFVLVTGIGLIATVRRRKAS"
     gene            complement(3674..4474)
                     /locus_tag="AC1LW8_001897"
     CDS             complement(3674..4474)
                     /locus_tag="AC1LW8_001897"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP4773710.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(4863..5651)
                     /locus_tag="AC1LW8_001898"
     CDS             complement(4863..5651)
                     /locus_tag="AC1LW8_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HIP4773711.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5652..6986)
                     /gene="yycH"
                     /locus_tag="AC1LW8_001899"
     CDS             complement(5652..6986)
                     /gene="yycH"
                     /locus_tag="AC1LW8_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HIP4773712.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDYDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(6979..8805)
                     /gene="walK"
                     /locus_tag="AC1LW8_001900"
                     /gene_synonym="yycG"
     CDS             complement(6979..8805)
                     /gene="walK"
                     /locus_tag="AC1LW8_001900"
                     /gene_synonym="yycG"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HIP4773713.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8818..9519)
                     /gene="yycF"
                     /locus_tag="AC1LW8_001901"
     CDS             complement(8818..9519)
                     /gene="yycF"
                     /locus_tag="AC1LW8_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HIP4773714.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         2661 a         1922 c         1465 g         3478 t
ORIGIN      
        1 tcttcaatat gtacttattt atattttacc gtaatttact atatttagtt gcagaaagaa
       61 ttttctcaaa gctagaactt tgcttcacta taagtattca gtataaagaa tatttcgcta
      121 ttatttactt gaaatgaaag actgcggggg ctaactatgt caaaaatcat gaacctcatt
      181 acttatgata agcttcttaa aaacataaca gcaattcaca taaacctcat atgttctgat
      241 acattcaaaa tccctttatg aagcggctga aaaaaccgca tcatttatga tatgcttctc
      301 cacgcataat cttaaatgct ctgtacactt gttcaattaa cacaacccgc atcatttgat
      361 gtggaaatgt cattttgctg aatgatagtg cgtagttact acgttgtaag acgtccttgt
      421 gcaggccgtt tgatccgcca atgacgaata caaagtcgct ttgcccttgg gtcatgcgtt
      481 ggttcaattc ttgggccaat ccttcggaag atagcatctt tccttgtatt tctaatgtaa
      541 tgactgtgga ttgtggtttg attttggcta gtattcgttg gccttctttt tcttttactt
      601 gctcgatttc tttgtcgctc atattttctg gtgctttttc gtctgtaact tctatgatgt
      661 ctatcttggt gtatgggcct aaacgttttt catattctgc tatggcttgc ttccaatatt
      721 tctcttttag tttccctaca gctaaaatgg tgattttcat gtcgtttggt cctccaaatt
      781 gttatcaact ttccagttat ccacaagtta ttaacttgtt cacactttcc cctcttatta
      841 taccaagttt ttttgcagtt tttgatattt ttctgacatt catgcccaat tttcagtgta
      901 taacttgtgt atttgtggat aactctgtgg gcattgtggg tatttctctt taaattgtac
      961 acaattctgg ataactatcc ccatttctgt ggataaatac cttgatgtct tattacttat
     1021 ccccgttttt atatcccata tttatcaacg gttatagctt ttttccataa aaaaacacta
     1081 ccgtacctct tatttaagaa atacagtagt attgttttca atatatttta gctagctttt
     1141 ctacgtcgta cagtcgcgat taaacctata cctgttacta atacaaacat cgctgctggg
     1201 cttgaacttt gatcagttcc agtttttggt aactgtttct catgtgcgct acctttaggc
     1261 gctgacatgt tagccaattg tttcccactc ttacttgata cagcagttcc ttctaatgcg
     1321 cgatcagaac cttctgtacc gctactaaca gttcctctat gcgctggtaa caacacaact
     1381 ttacctgtcg ctggttgttt cgctggattc atcactttct ctttaccaat tggttgtgta
     1441 tctttaccag actcactacc tttgctacct tgttgtcctt tagctggttg ttcacttgct
     1501 actggtttac ctaataacat acgttgtggt tctgtcatat catacttagc taagttagct
     1561 gtttttaaat aacttgctag tacttgatct aatgaaatac cttcttctct agggccaccg
     1621 aacatactat atccgtctcc acctgatgct gtgaagtcat tcatcgttac atgatataca
     1681 cgttttaaat caatactttc aaacttacct gtctctttat ttaaaatttg aatagcatta
     1741 attcgtttgc cagacggttt attcatatca tagtaaacac gaattgaatc agagatatgt
     1801 aataaaccgc cattcgctgt taacactgtc ttaccgtctt tttgtgttgt tggtgcacct
     1861 aaactatgtt cgaaagccgt ccagacgtct gaacctttta catcaatttg cgcaatcgta
     1921 tttccaaatg gtaatactga gattaaatca tagcgtgtca ccttaccttt tgcgatagag
     1981 gcacgaatac ctccaccatt tgtcacggca aagtcagtct ttttagagaa attcttaacg
     2041 ccatatgctt ccatagcatc tgcaatcgcg tttcctaaat ttgtttcacg cgttctaacg
     2101 tcatctcttt ctcctttgaa atcaatcgta ttatttggaa taataacctc tgctgtttgt
     2161 gctctaaatg tttgatcagc ttgattaatt tgttcagcta atgctttgtt cggtgttaca
     2221 ttttcaacgt ctttaacatt aatcaatgac ggcttaatat tcgatacttc tccattgcga
     2281 taattaaatg taatcttacc gatattcgca agtgctgtac ctgtttgtac taatgcatca
     2341 ttgttataaa tttgaccatt ttgaagtacg gtatgtgaat gaccgtcaat cactgtaata
     2401 cgtttcttca attgtggatt ttgacttaat tgtttcacta agtaatcgcc acgccatgtt
     2461 tcttgtgttg aaggatcaat acctaaatga gatataacaa caaatgtatc tacatcttta
     2521 taaatacgca tcatttccgc tgtcacactt tgtaatggat ctctaaattc aacaccttta
     2581 atgccttcag gtcttgtttt cgtctttgtt tctggtgtcg ttacgccaat aattccataa
     2641 cgaataccat ttttcgttac aatcgttgaa ggcttaaacg cgcgttttcc atctttataa
     2701 acgttagtac ttagcatcgg gaagtctaac ataccctcta actttttcaa ctgatcgtat
     2761 ccaaagtcaa attcatggtt accgactgcc atagcatcat aacctactgc attcattgct
     2821 ttagccattt cttcaccttt agattgattt gaaagtggta aaccttggaa ggcgtctcct
     2881 gcgtctaaca ttaaatcagg cttttcttgt tcttttactg tttttaattt agccataccg
     2941 atgacacgcc ctttttcttc ggctagtcgg ccatggatat catttgtatg taatatttta
     3001 tgtgttgttg tttgtggtac attagcagca aacattcgtg gtgaaagtga tgctgtttta
     3061 gcatttgata atttgaatgt tgctgtgtga gttggttttt gtgttgaggc ttgttgtgca
     3121 tctacatcgt gtcttttgtt tcctgttgtt gaaactgctg tagttggttt gttagttggt
     3181 gcgttggatg ttactttgtt gtttgtatca tcagctgttc cctgcgctga tgctgatacg
     3241 ttggttgccg cttcttcacc ataatgatga gccgcttcct ttgttggcgg tacttgttgc
     3301 ctatctgttg ttgctttgtc tatcgttgtt actgcatgtg ctttcattgg tgtatgctgc
     3361 tcagccgcgt tcgagacttg ccataatccc atcacactaa aaagcaaaac gagccataca
     3421 cttgttttaa gtaataaagc tttcatttcg ttccctccat taactgtcat acaccaatat
     3481 attatcataa tccttatatt aaaatgattg attttatgta aattatttat aaattacatt
     3541 taatatcgaa ttattttaca catttcaatc aagccggttc aatctaacaa cagccatttc
     3601 ccaccaataa cataaaaaag cacccgataa agtcgtctca cagcagtgcc gaactttata
     3661 gggtgactct catttatatt gtatatattg gcgttggaat agctttatcc gtatcacata
     3721 gcaatacttc tttttccgta tcaatatcgt gttcgttcaa tacttgacca acactcatac
     3781 gcgccaaatc tttcatatta ttatcttgtg ataaatgtga taggtaaata cgtttcgtgt
     3841 tgcctgtaat cacatccgtc atcgcatggc ccgcatcctc attagataca tgtcccatat
     3901 cgcctaaaat acgttgtttc gtcttccatg gataacgaca cattctcaac atatcgacgt
     3961 catgattact ctcaaaaata aacgcatcgc tgccacgtat catacctttc atacgatccg
     4021 acacatatcc cgtatccgtt aaaatcgtaa acttcttata gttattatgg aaaatataaa
     4081 attgcggatc tatcgcatca tgtgacacgt taaacgattc aacatcgaaa cctgcaatag
     4141 atttcgtttc ataaggatta aaaatgaatt tctgatccat agggatgcga ctatctttct
     4201 tttcaatagc ttgccaagtc ttttcattcg cataaattgg caattgatat ttacgcgcta
     4261 aaacacctaa acctttaata tggtcaatat gttcatgggt tactaaaata ccatttaaat
     4321 cttgaatatt acggtcaatt tgactaaaca attcttccat tttcttgcca gtcaaaccaa
     4381 catcaactaa tagactacct ttttcatttt ctacaaaagt ggcgttacct gtactaccac
     4441 ttgctaatac actcatgcgt atcaagcggc tcatcctttc tatttaagca gtcaatctct
     4501 ctatatgact gcaatataca tcagtgcaaa tatacgattt tccctacaga aactatcagt
     4561 atattagctg ttttgtatac ctttcctatt atgtaacttt agttcatcga ccggtctctt
     4621 ataattaaat tttgataatc acgtgcaatg aatgtcctaa ttattataac aatgattaga
     4681 caaatactaa agtttttaaa ataacagtta cactaatttt acaatgcggt actacttaaa
     4741 atatgaataa atgcgcacgt tggagacaca gaaacgtcag ccataataaa ccgtaccagc
     4801 aaaaagctag caccgcttct cacatatgat gagcttgcaa tgctagctta ttacgattca
     4861 tattaatgat taataatttt agggttatta tttgtcgctt cgacatagta agtattcgtt
     4921 ttgtccttac cgtcatgctt cactttaatt tcccagtttg gttgtaacaa ttgaacattc
     4981 gtttcattca cgactgaata gtaaccaagt ctagcattaa tgacttcatc accttttttc
     5041 aagtaacgat tataatataa tgcctcgatt gcttttctca caccaatcac ttgatgcttc
     5101 ttatctgcac catctgtggg cttaatgtca tccatcattg attgtttata actagtcgct
     5161 ttgttatctt ctatattaaa gttcaacatc gctttactat tgttcaaaat cgggaaatta
     5221 tcatacgttt gttcgtattt tacagaacca gaactaattt cacttaattg atattctgaa
     5281 cctttgaaca cacgtttgtt aacataatct ttcaaatctt ttaagttatt gtcactgacc
     5341 tttaccgatt gactaatgtt cgcatttaat aattttccac catctgatgt ggtcaaatcc
     5401 gaatcgcctt tagctttaga actaaagtct tttgatcgcc ctgtaatttg ctctaatttt
     5461 atacctttaa ctgatttatt caatatacta gccggtactt taatttcttc ttgctgaaaa
     5521 ttaacttcat tgttactctc aacttcatta atgtgtgagc gattgacttt attaacataa
     5581 atcgatacta acacgatgtt gacaagaata aacacgaaaa tgaaaagtgt ctttgtcagt
     5641 ttccagttca tttattcaag cctcccatcg ttataaacat accattcgcc atcatattct
     5701 acataccaac gcggtacgag ttcactgtta atctgcactt caatgtgatt atgatctgag
     5761 ttatcctgca tttcgtaacc gatagcgatg tttgttactt tttcaaaatt aatatcacta
     5821 ttgttcgcta agcttgaacg tacagactct aatttcggca acgttttatt atcttcactg
     5881 tttaaaacaa cgtcggtgcg taataacgaa cgacgataat caaagacgcc tttttcaccc
     5941 caagtgactt gaatttgatt agagccttct ttattaaacg ttggataacc attaaggaaa
     6001 cgttgatatg ttaactcgcc tgactgatta ttcgtactaa acaatctgaa gtcttcgttt
     6061 aagaaaccac catgaccatt aataaaatca aaggttcctg gaatcgtttc ttccattttg
     6121 ctggaactcg cttcatcttc ggacaagttt ttataatgat atttttcatt tttatcgtta
     6181 tagtttgcga cacctgtatt attgttgtag gttgtaacac cactctttga actacgaacg
     6241 atggttgaat cgtcaaatag tatagcattc attttctcaa cactaatcgt gttaaatacc
     6301 atgcgatatg tttttaactt ttcaggttta cttggtgcaa aaacatgcgt cgtacgatca
     6361 atcgtatctt tgtttgtgat gatatctgtg tatggttgca tatctttttt cactgctgct
     6421 aatgcatcta aaaaatgatc atttttcgtt gtagttgtta attttacgta atcgtggcga
     6481 tctttgctta tagcataaag cacgatatta tcatcagcat cataatctat gaccaaacga
     6541 ttgaaattga aatgatttgg tactttcgca ttcatgttca gtacttggcc aagatatgtt
     6601 gataacggta aatcatacgt aaaatcgaat aatgtaaaat cactgctcaa atcaggaatc
     6661 atcaagttat gatcccgacg aacatgttcc acggacttca cttctttatt tttcaaaggt
     6721 ttcgtcagtt tattcacatt agataccgtc gcgattgtac cttctggatg atcatttttc
     6781 gaatgaataa tctgaaatgg cgtaatagtt gtatccattt tggctgtcat aggtgtcgtt
     6841 aaaggtttcg tttcactctt cttactatct gtattgtcga catttgcaat atcaggagaa
     6901 aagttccata ccatatatgt caatacgaca ctcatcaaga cgagtagcgc taaaatgaca
     6961 gatttaatat gttccttatt attcatccca atcaccgtct tcaatgactt cacatggaag
     7021 tgtgataaag atagatgtac cttgaccttc tacactgttt gcccaaatac gaccattgtg
     7081 cgcttccaca atctctttcg aaatggctag tcctaatcca gtaccaccca ttttacgcgt
     7141 acgcgcctta tctacacgat agaatcggtc gaatatctta tcgactttat tgataggaat
     7201 accgatgcca ttatctttaa tacgaatcgt cattcgatta taaagtggat tttgtttcac
     7261 gtggaactcg acacgtttat cgcctctaga atatttcatc gcatttgtaa tgacattatc
     7321 aaatacttgc gtcattttat caggatcaaa ttctgtgaaa atcgtctttt tcggaatatc
     7381 tcgaataaat gttgtatctt tcgcagacat ttcatgtcga ttaataattt tattaatgaa
     7441 catgttaaag tcgataattt ctttgttgat ttgatcagac tcattatcca ttttagataa
     7501 ctgtagcaag tcattgacca gtcgaatcat tcgttctgtt tcttcacggg taacagataa
     7561 aaattgtggc gcaagttcct catctttcca tgcaccttct tcaagtgctt caatgtaact
     7621 attcatagaa gttaaaggtg tacgtaactc atgtgataca ttggcaacaa attcacgacg
     7681 ctcacgttca acttgttgtt gttcagtaac gtcatgtaac acagcgatat aaccagttac
     7741 aaatcctgtt tcctgcacaa tcgtactaaa gttaacacgt gcgattagac cttcttcttc
     7801 atttaaatct aataagaaac tatcattatt ctcttgaatt tcttcaagtt taaattcatc
     7861 ttcaagactt aatacactta acatgtaata tccaatgatg tcttctttcg ccataccaag
     7921 catcttgagt gccatatcat tgacgatacg aatacgtcca cggcggtctg ttgcaataat
     7981 accatcactc atatgggtga taactgagtc cagtctacgt ttctcacttt cagtattagc
     8041 ctgcgcttct tgtacacgtt tagacaagtt attaaatgct aaagctaatt cgccaatttc
     8101 atcattacca taaatcttca cacgttgcgt atagttacct ctggacattt caaccgtctg
     8161 gttacgcata tcggtgattg gtttggtaat cgttcgcgct ataaagaatc ctaggatgac
     8221 tgtgattaat aatgaaatag ctgtaccaac aatgaatatt tgatttatat tatttaattg
     8281 gttataaacg tcattaattt ttgattcgat ataaatatta ccaattacct ttttatcgac
     8341 tttaactggg atattatata cccagacacg gtccttacca ccgccataat cttttaaaat
     8401 taaatgatcg tttgattgtc ctagtgatag tgctttttgg acagaactat cattcgcttt
     8461 ttgattgatt agactacggt tagactgctt cgtcgtcgca ataataattt ggtctttatc
     8521 tataaaacga atttctccaa tttcttgacg gttggcatac tcacttaata aattttgaat
     8581 atctttttgt gcatttacgg agcccttttc gtcatatact ttttcaatac taatttctaa
     8641 ttgtttagcg tactgcgtaa tattcttctt aaaattatca agcagctctt tttcaagatt
     8701 atttgtaaaa tacagcccga taatttgcat accaatgata atcagtaata cataaacaat
     8761 tacaagttta gtatgaaggg attgtagttg ttttagccac ttcattcgtt tcgacctcta
     8821 ctcatgttgt tggaggaaat atccaacgcc tctacgcgtc acaatatatt caggatgtga
     8881 cggatcatct tcaatctttt cacgtaaacg acgaatcgtt acatcgaccg tacgtacatc
     8941 gccaaagtaa tcatagcccc atactgtttg taataaatgt tcacgtgtca ttacttgtcc
     9001 catatgtttt gataaataat ggaacaattc aaattcacga tgtgttaatt caatatcttc
     9061 gccacgtttt ttaatagaat atgcgtctgg ataaatcaca atatctttaa ttgtgatttc
     9121 attcgttaca tttccagtgt cttgtgctgg ttgtgagtaa tgacgacgta agttcgcttt
     9181 cacacgtgcg attaattcac gcgtactaaa cggtttcgtt acatagtcat ctgcacctag
     9241 ttctaaacca agcactttat caatttctga atctttagca gtaagcatta taattggcat
     9301 ttcgtatttt ttgcgcactt cacgacatac ttccatacca tcacgaccag gtaacatgat
     9361 atctagtaat acgatgtctg gttcttcttc ataaattaag tcgactgcat cattaccatc
     9421 gtatgcacag tacacatcgt atccttcttt ttttaagtta aattctaaaa tatcagcaat
     9481 cggtttttca tcatcaacta caacaacttt tctagccatt tgcata
//
LOCUS       DBNENW010000100         9510 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNENW010000100 DBNENW010000000
VERSION     DBNENW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.101"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            130..594
                     /gene="xdrA"
                     /locus_tag="AC1LW8_001902"
     CDS             130..594
                     /gene="xdrA"
                     /locus_tag="AC1LW8_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator XdrA"
                     /protein_id="HIP4773715.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            773..1897
                     /locus_tag="AC1LW8_001903"
     CDS             773..1897
                     /locus_tag="AC1LW8_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIP4773716.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLFGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1966..2310
                     /locus_tag="AC1LW8_001904"
     CDS             1966..2310
                     /locus_tag="AC1LW8_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIP4773717.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2803..2901
                     /locus_tag="AC1LW8_001905"
     CDS             2803..2901
                     /locus_tag="AC1LW8_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773718.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            3192..4388
                     /locus_tag="AC1LW8_001906"
     CDS             3192..4388
                     /locus_tag="AC1LW8_001906"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIP4773719.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKVQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4378..7314
                     /locus_tag="AC1LW8_001907"
     CDS             4378..7314
                     /locus_tag="AC1LW8_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP4773720.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7311..8252
                     /gene="yhaM"
                     /locus_tag="AC1LW8_001908"
     CDS             7311..8252
                     /gene="yhaM"
                     /locus_tag="AC1LW8_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HIP4773721.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRVAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8373..9335)
                     /gene="prsA"
                     /locus_tag="AC1LW8_001909"
     CDS             complement(8373..9335)
                     /gene="prsA"
                     /locus_tag="AC1LW8_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HIP4773722.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3532 a         1354 c         1624 g         3000 t
ORIGIN      
        1 aaatgtgaaa ttgactcttt tcattttatt aaaaatatat taagatgtaa tctgtgtttc
       61 aagaaatgtg tattattgca atttcttgtg tccggataaa attccccaaa cagaaaggta
      121 gataacagaa tggatagaca gagttttaca gatttaattc aaacaaaatt taaaatggtt
      181 cgtatagagg ctggttatac gcaagatact atggcgcaaa caattggact ttctaaaaag
      241 actttagtac aaatcgaaaa agagagagta ttaccaaact ggacaacttg tatttcaatt
      301 tgtgcattat tcagagactc tgaagtatta aacagtacat ttggctgtga tccattagaa
      361 atcgttcaaa caatttcaag aaatcactgt gcatatccaa accatgcacc aacaagtgat
      421 atttattgga ataacattga gactcgtaat gggtacattt tacaaagtaa caaagtaagc
      481 aatatttatc gtgtattaaa tccagataac caaccaattt tcggtacttc taaaatgaga
      541 gaagctgaaa catactttaa cagaaatgct aaagaagaat tagtacatat ctaggcctat
      601 atacttttaa taaataataa aagcgatgta acacacctgt ttcgagaggt gaggcatcgc
      661 tttttgtatg taaactaata ataaattgag cagaaagtga aatgattaat gaattaaaaa
      721 ataaaaatag tggtataatg aataactatg ttgtcacaaa ggagtcaaaa ttatgaatgc
      781 actatttatt atcatattta tgattgtagt aggggcgata attgggggca ttactaatgt
      841 aattgcaatt agaatgctgt ttcacccctt taaaccatac tatatattta aatttagagt
      901 cccatttaca ccaggtttaa taccgaaaag acgcgaagaa attgcaacta aaattggcca
      961 agtgattgaa gagcatttgc ttacagaaac tttaattaat gaaaaattaa aaagcgagca
     1021 atcacagcaa gcaatagaat ctatgattca acagcagtta caaaagttga cgaaagatca
     1081 attgtcaata aaacaaatta cttctcaaat cgatattgat ttagaacaag tattacaaac
     1141 taatggaaat caatatattg aatcacaatt gaataattat tatacaaagc atcaaaacca
     1201 aacaatagca tctttattgc caaatcaact tgtaacattt ttagatcagc atgtagataa
     1261 cgcaacagac ttattatgtg atcgtgcaag gaattattta tcatctgcaa aaggcacaca
     1321 agatattaat gatatgttgg atactttttt caatgagaaa ggtaagttat ttggtatgtt
     1381 gcaaatgttt atgacaaaag agagcattgc agatcgcatt caacaagaac ttatacgttt
     1441 aacatctcat cctaaagcaa gaacaattgt gacatcgtta attaccaatg aatatcaaac
     1501 ttttaaagat aaaccattga atgaactgtt agatgcatcg cagtttaatg aaatcgctga
     1561 aaatctatca gtgtatgtaa caacatatgc aagcaaacaa gcgaataagc cagtggttac
     1621 gttaatgcct caattcgttg attatttaga aggtcaactc tctagcaaat tagcaaattt
     1681 aatcattgaa aaattatcga ttcacttatc tacaattatg aaaaaagtgg atttacgagg
     1741 attaattgaa gaacaaatta atacgtttga cttagattat attgaaaaat taattataga
     1801 aattgctaac aaagaactaa agttgattat gtcattaggt ttcattttag gtggtattat
     1861 tggatttttc caaggtttgg ttgcaatctt tgtctaaaaa agcgagatgt ggtaaagtaa
     1921 tatcgaacgt tttaataaac atcacaacaa aggagtgcta taaccatggc agtaaattta
     1981 tatgattatg caaatcaatt agaacaagct ttaagagaaa gcgaagaata caaagcaatc
     2041 aaagaagcat tcgctaatgt aaaagctaac gaagaatcta aaaagttatt cgacgagttc
     2101 cgtgaaactc aaattaactt ccaacaaaaa caaatgcaag gtgaagaaat tgctgaagaa
     2161 gatttacaaa aagcgcaaga acaagcgcaa gcaattgaaa aagatgaaaa catctctgca
     2221 ttaatgaatg ctgaacaaaa aatgagtcaa gtattccaag aaatcaacca aattatcgtt
     2281 aaaccattag acgaaattta cgctgactaa tattaaaata atatttttca atcctttaaa
     2341 tagaggaata gactgccgac agagtccgag actttgtggg tagttttttt agttttgata
     2401 acggaagtta gaggctctct gtcaaattgg gcagatgcgt tcagtatcgg aatttaggct
     2461 actaaacatg tcctagcttt agctcagaat ggacgacgga agctgggcaa tcaaacatgg
     2521 ctagctctat gccaaaacgg gcagatgagc ttgataacgg aagttaggct agtaaacgtg
     2581 tcctaacttc ctttttgcat tttgaagtgt aaagttttca acataatact attagttcgg
     2641 tcatgtatcg gactgatgga aaagcgtttc acttttaatg gctcattaag aacggcctga
     2701 aaatgtttgg cgtatttagt gcaatgatag ttttgacatt tagtttctaa ttggtcatta
     2761 ctgccgagca aatctagtag agtaatcatg taaatcttta atgtgtcagt tggttcacta
     2821 gcggtgttaa caattacgga aattgcattt ccgactgaaa tttttgaaaa aaatcaacgc
     2881 acgctacaaa taaaatttta aactgttata aatgtgtctc aatttcatat gttcatcgac
     2941 gatatgaagc gtattatggt aaaatgaaga aataataaac ttgttaataa ataaaacatc
     3001 acgatttgac taaagcactt tattattgtg tagataataa ttttttaacg aaataaaaat
     3061 ggcgactggt tttaataaat cagctaatga atcactacac ctataagtat gaatatagtg
     3121 attagaatgc tttgtatagt tggattttgc aaaattgatg ttagaacctt taattaagga
     3181 gatatggatg tatggttaaa tttattcatt gttcggattt acatttagat agtcctttca
     3241 aatctaagag tcatataagc cctaaaattt ttgaggatgt tcaaaaaagt gcttatgaaa
     3301 gttttaaaaa cattgtagat attgcattac aacaagatgt tgattttgta attatcgcgg
     3361 gtgatttatt tgatagtgaa aacagaacat tacgtgctga aattttctta aaacagcaat
     3421 ttgaacgttt acaaaatgaa caaatctttg tttatgtttg tcatggtaat cacgacccat
     3481 tatcatcaaa gatttcatca aactggccag ataatgtttc tgtattttca aataaagttg
     3541 agacgtatga agcaattact aaatctggtg aaacaattta tattcacgga tttagttatg
     3601 aaaatagagc aagttatgag aacaagattg atgaatatcc atcaagtcaa ggccaaaaag
     3661 gcatacatat tggtgtcttg catggtacgt atagtaaatc ttcagttaac gaaagatata
     3721 ccgagttcat tttagaagat ttaaacagta aattgtatca ttattgggct ttaggtcata
     3781 tacatgaacg tcaacaatta agtgatatgc ctgtaattaa ctattcaggt aatattcaag
     3841 gtagacattt taatgagcaa ggtgaaaaag gttgcttatt aatcgagggt gaccacttaa
     3901 aattaaagac taaattttat cctacacagt atattagatt tgaagaagca actattgaaa
     3961 cggataagac atctaagcaa ggtttatacg aggtcattca aaactttaaa gaacaagtga
     4021 gagaagaagg aaaagccttt tatcgtttaa cgcttgttat taatagtgag acattaattt
     4081 cacctcaaga tttattacaa gttgaagaaa tgattacaga ttatgaagaa aacgaaaatc
     4141 aatttgtata tattgatgag ttaaaagtac aatatgcaca aaatgatgag tcacctttag
     4201 ttaatgaatt ttcagcggaa ttattagtcg atcaaactgt ttttgataaa gcgatgtcag
     4261 atttatattt aaatccaagg gcatctaagt tcctagacga ttatggaaca ttcgaccata
     4321 cagcattagt taatcgtgct gaagaaatat taaaagctga aatgagaggt gaacaaaatg
     4381 ataattaaat cacttgaaat ttatggttac ggtcaatttg ttcaacgtaa aattgaattt
     4441 aataaaaact tcactgaaat ttttggtgaa aatgaagcgg gtaaatcgac gattcaagca
     4501 ttcatccatt cgatattatt tggttttcca actaaaaagt ctaaagaacc aaggctagaa
     4561 ccacgtctag gtaaccaata cggtggtaaa ttagtactta ttcttgatga tggcttagag
     4621 attgaagttg aacgaattaa aggcagtgct caaggtgatg tgaaagtata tttacctaat
     4681 ggtgctgtgc gtgatgatgc ttggttacaa aagaaactta attatatttc taaaaagaca
     4741 tatcaaggta tcttttcatt tgatgtacta gggcttcaag acattcatag aaatctaaat
     4801 gaaaaacaat tgcaagatta tttattacaa gcaggggctt taggatcaac tgaattcacg
     4861 tcaatgcgcg aagtgattaa tcgtaaaaaa gatgaattat ataaaaaatc aggtaaaaat
     4921 ccgatcatta atcaacaaat tgagcaatta aaacaactag aaagtcaaat tcgtgaagaa
     4981 gaagctaagc tagaaacata tcatcgctta gtagatgatc gagataaatc atcacgtcga
     5041 ttagagaatt taaagcataa tttaaatcaa ttatcaaaaa tgcatgaaga aaaacaaaaa
     5101 gaggttgctt tacatgatca ttcacaagaa tggaagtctc tagaacaaca gttaaatatt
     5161 gagccaatca cattcccaga aaaaggtgtg gatcgttacg aaaaagcacg agcgcataag
     5221 caatcgttag aaagagatat tggtttaaga aatgagcgtt tagctcaact taaagaagaa
     5281 gcgactcaat tagagccagt taaacaatct gatattgacg ccttcattag tttgaatcaa
     5341 caagaaaatg aaattaaaaa taaagaattt gaacttactg caatcgaaaa ggatattgcg
     5401 aataaacaac gtgataaaga tgaattgcaa gcaaatattg gttggtctga aacgcatcat
     5461 gacgtagata gttcagaggc aatgaaaagt tatgtcagtg agcaaatcaa gaataaacaa
     5521 gaacaagctg catacattaa acaattagaa cgtagtttag aagaaaataa aatcgaagat
     5581 aatgcggttc atagcgaact agattctgtt gaagaaaaaa tagttcctga agagactttt
     5641 gaaaagaaaa aagaatactc acaacaagtc attgaattaa acgaaaaaga gaacttgtat
     5701 agcaaattga aagaacgttt cgaaattgag caacaagaaa aacaaaaacg tcaaaaattg
     5761 ttacgaacaa cgtttattct tttaacatta gtaggtattg gtttaactgc cttctcattt
     5821 atttcaaaca acatgttgtt tggtattatt ttcgcagtat taactttagt atttgttatc
     5881 ggtatcatca tgtctaaatc aaaagaagta gattatagcg aggccatcac agatgaaatc
     5941 gaagaaatta aagcacaact agccatttta gatgaaaatt atgacttaga ttttgatctt
     6001 gatgagcaat atcgcattcg tgatcactgg caacaagctt tgaaaaataa agatatttta
     6061 gaagagaaac gtcagtatat tgaaggtcgt ttaaatgatg cgaaagggcg tcatgatgaa
     6121 ttacaatcga cagttgagaa cgttaaggat gaattatact tatcatctaa aatttcaaat
     6181 gatttaattg ttgatagtat ttctacaatg gcgaatatta aagcattaga tcaacatatc
     6241 agtgatttaa atcaacaacg ccagcaattg gttcaagaat tagatacatt ctacaatcat
     6301 gctgaagctg ttacaaaatc acaatttgtt tatttcaaca aattatcttt attccacgat
     6361 gtgcaacaat ggttgaaatc agctgaagac acgaatgaaa aatggcgtat taatgctgaa
     6421 aataccaaac ttgttacaaa tgaattaaat catttaaatg ctcaattaga agaaaataat
     6481 aaagaaatta cagcactatt cgattttatc aatgttggta ctgaagaaga tttttaccaa
     6541 catcatgaag attaccaaac ttatactagt aatttgagtc gttttaatga tttaactaaa
     6601 tatcttgaga accaaaacta ttcttatgaa ttaagttcta gtttaagtga aaaaacaact
     6661 gcacaacttg aagaagaaga tcatttattg gctactcaag ttgacgaata taatgagcaa
     6721 tatcttgaaa tgcaagcaca agtcagtgat ttaagtgcac aaatcaatca catggaaact
     6781 gatacaacgc ttgctaattt aagacatgaa tatcacagtc ttaaaaatca acttaatgat
     6841 atcgcaaaag attgggcaag tttaagttat ttacaaagtt tagttgatga acacattaaa
     6901 caaattaaag ataaacgttt gcctcaagtt attaatgaag cggtagaaat attgaagcat
     6961 ttaacagatg gcagatatac gatgattaac tataatgaag attcaattac ggttaaacat
     7021 gttaatggtc aattatatga tcctgttgaa ctaagtcaat ctacaaaaga attactttat
     7081 gtagctttac gtatcagttt aattaaggta ctaagaccat attatccgtt cccattaatt
     7141 gttgatgatg catttgttca ttttgataaa aaacgtactg aaaaaatgtt gaattattta
     7201 cgatcattat cagaacacta tcaagtacta tactttacat gtgtaaaaga taatattgtt
     7261 ccatcaaaag aagtgattac attaaacaaa atagaggaag gcgggaaacg atgagaaata
     7321 tagagaatct aaatcccgga gattcagttg atcacttttt cttagtgcat aaagctacac
     7381 agggtgtaac agcacaaggt aaagattata tgacattaca tttgcaagat aaaagtggtg
     7441 aaattgaagc gaaattttgg acggctacaa aaaatgatat ggcaacaatc aagcctgaag
     7501 aaattgtaca tgttaaaggt gacatcataa actatcgcgg aaataaacag atgaaagtca
     7561 accaaattag actagcgaca actgaagatc aattaaaaac agaacaattt gtagatggtg
     7621 cacctttatc accggcagaa atacaagaag agatttctca ttatttgcta gatattgaaa
     7681 atgctaattt acaacgtatc acacgtcatt tattgaaaaa atatcaagaa cgattttaca
     7741 catatccagc tgctagttct catcatcata actttgcgag tggcttaagc tatcatgtat
     7801 tgacgatgtt acgtgttgca aaatcaattt gtgacattta tccattgtta aacaaaagtt
     7861 tgttatatag tggtattatt ttgcatgata ttggtaaagt tagagaattg agtggtcctg
     7921 ttgcgacgtc gtatacagtc gaaggtaact tattaggaca catctcgatt gcgagtgatg
     7981 aagtagttga agcagctcgt gaattgaaca ttgaaggaga agaaatcatg ttgttaagac
     8041 atatgatttt atctcatcat ggtaagttag agtatggttc gccaaaactg ccatacttaa
     8101 aagaagcaga aattttatgc tatatcgata acatcgatgc tagaatgaat atgtttgaaa
     8161 aggcttataa aaaaactgac aagggtcagt ttacagataa aatatttggt cttgaaaatc
     8221 gtagattcta caatcctgaa tcactcgatt aagaattaaa agatatcgga cagatgagta
     8281 tactgaaaca tgagctcatc tgtccgattt ttaatgcgaa aaaaccgagc ggccgtggta
     8341 tgatttttga accacggtcg ctcggttttg tgttattggc tcatgccgga ttgtccgcct
     8401 tgtgcgccac cttgtttaag tttttcaggg tttaagattt tatcttcgac aactgattta
     8461 atatcacgat ctttaaagtc aacatcgtat tcttttaata gatctttgta tgcatcagtt
     8521 aataattttg gatttttttg tactttctga tcgactaatt tttctttcag gctttgtttt
     8581 tcactgttaa agtctgttgg tttatcagct ttaataatat gatatccaaa gcttgattta
     8641 acaacctctg atacttcacc atctttaagc ttaaatagtg ctttttcaaa atctttatca
     8701 gtttgtcctt taagaacata acctaattcg ccatcttttt tagctgaacc agtatccatt
     8761 gattcttttt tagcgatttc accaaattta cttggatctt ttgaaacttc tttttgaatt
     8821 tcttcagctt tttgtttcgc ttctttatcg tctaagcctt ctttgtcgct tttcttagat
     8881 ttaactttaa ttaaaatgtg tgaagctttc ttgctgtctt ctttaatttc agaatcagag
     8941 attttaattt tatctgatag taattcttta tgataagcag cagtacgtaa attttcttta
     9001 tatttatcgg ctgttaaacc ttgctgttga agggcctttt caaatttatc tttaccgccg
     9061 tattgctttt gcattttttc aatttgttcg tcaatcttct tatcattaac tttattttta
     9121 tatttatcag ctaaaatttt atttaacatt tcagtaaatg atgcatttgc aatttgatct
     9181 ttaccgattt ttttcattgt atctgcaact gttacgtctc cagctttaga agaaattaat
     9241 gtattttctt tagagtctgt ggcactagcg ccacaagcgc ctaataataa agcactagct
     9301 gttaccggaa cgattaattt gtttatcatc ttcatagttg aaactccttt gtaagttaat
     9361 catcgtgaat ataacatagt tttatattat caccaatcaa taacccatga catcagacta
     9421 gagcattagg tttaggggta gaaattgatg aaaataaaaa aacaaggtct atatacattt
     9481 gattgtaagc tattacatag cctaaaaaca
//
LOCUS       DBNENW010000101         9435 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNENW010000101 DBNENW010000000
VERSION     DBNENW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.102"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..257)
                     /locus_tag="AC1LW8_001910"
     CDS             complement(<1..257)
                     /locus_tag="AC1LW8_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HIP4773723.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMP"
     gene            384..827
                     /locus_tag="AC1LW8_001911"
     CDS             384..827
                     /locus_tag="AC1LW8_001911"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773724.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            894..1289
                     /locus_tag="AC1LW8_001912"
     CDS             894..1289
                     /locus_tag="AC1LW8_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA0632 family lipoprotein"
                     /protein_id="HIP4773725.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            1427..1726
                     /locus_tag="AC1LW8_001913"
     CDS             1427..1726
                     /locus_tag="AC1LW8_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773726.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            1806..2348
                     /locus_tag="AC1LW8_001914"
     CDS             1806..2348
                     /locus_tag="AC1LW8_001914"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773727.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(2444..3010)
                     /locus_tag="AC1LW8_001915"
     CDS             complement(2444..3010)
                     /locus_tag="AC1LW8_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIP4773728.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(3012..3470)
                     /locus_tag="AC1LW8_001916"
     CDS             complement(3012..3470)
                     /locus_tag="AC1LW8_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIP4773729.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(3473..4156)
                     /locus_tag="AC1LW8_001917"
     CDS             complement(3473..4156)
                     /locus_tag="AC1LW8_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773730.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(4328..5203)
                     /locus_tag="AC1LW8_001918"
     CDS             complement(4328..5203)
                     /locus_tag="AC1LW8_001918"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIP4773731.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQITDIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            5422..7053
                     /locus_tag="AC1LW8_001919"
     CDS             5422..7053
                     /locus_tag="AC1LW8_001919"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIP4773732.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDN
                     SDRLVLNDLNLEIYNGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            7050..8723
                     /locus_tag="AC1LW8_001920"
     CDS             7050..8723
                     /locus_tag="AC1LW8_001920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HIP4773733.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLVVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKNRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8850..9293)
                     /gene="mgrA"
                     /locus_tag="AC1LW8_001921"
     CDS             complement(8850..9293)
                     /gene="mgrA"
                     /locus_tag="AC1LW8_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HIP4773734.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         3211 a         1386 c         1602 g         3236 t
ORIGIN      
        1 ttaggcatcg cttttagttc ttttattgca tgtgcttttg ggtcgtgatt gaaaaaatct
       61 aaggctaata aaccatgttg ttctgcgctt aataattgtt tgagtatacg gttaataatt
      121 aactctgtat catgagggtt gacgcgaaag tcagagcgca tataagtcat ataattctcg
      181 aagatttctc tatcagtatt gcttaatctt aatgatttaa cattattttc ttttgttaat
      241 tgcgcagtac ttttcattgt tacttaagcg ctcctttaaa aatgtttaat tccaaattaa
      301 aatggaaatg attttatagt attaataagg tcaatcatat catattaaac gcataaatat
      361 aacgattaat attggagagg aaaatgagga cacttaataa agatgaacat aattatatca
      421 agcaaatagc taatatacat gagacattat tgtcgcaagt agaatccaac tataaatgta
      481 ctaaactgag tattgctctt aggtacgaga tgatatgttc aagattagaa catacaaatg
      541 ataaaattta tatatatgaa aatgaaggtc aattaatagc gtttatttgg ggacatttta
      601 gtaatgaaaa aagtatggtt aacattgaac tgctatatgt tgaaccacaa tttcgcaaac
      661 tgggaatagc tacgcaactg aagattgcgc ttgaaaaatg ggcaaaaact atgaatgcaa
      721 agcgaataag caatacaatt cataaaaata atttgccaat gatatctttg aataaagatt
      781 taggttatca agtgagtcat gtgaaaatgt ataaagatat tgattagaat taggattatg
      841 ttgctaattc atgttaaaat taaaaaagat ttaatgacgt taaggagttt tatatgaaga
      901 aattaatcat cagtattatg gcggtcatgc tatttttaac aggttgtggt aaaagtcaag
      961 agaaagccac tctggaaaag gatatcgata atttacaaaa agaaaataaa gaattaaaag
     1021 acaaaaaaga aaagcttcaa caagaaaaag aaaaattagc agataagcaa aaagaccttg
     1081 aaaaagaagt gaaagattta aaaccttcaa aagaagataa caaggatgat aaaaaagacg
     1141 aagacaaaaa taaagacaaa gataaagata aagaggcatc acaagataag caatcaaaag
     1201 atcaaactaa gtcatcggat aaagataatc acaaaaagcc tacatcagca gataaagatc
     1261 aaaaagctaa tgacaaacac caatcataat cgaattgctt acttgttata gatgaaaggt
     1321 acagcgtttt aaaccttatt ttaagggtat gtattaatta aaatgtggtc atgattgaaa
     1381 cagaacgtaa aaatagacaa cgtaattaat aaaggagaga aacggcatgc atgaacaaga
     1441 ttttagaatt ttagagggtc aagatattac tttgccagaa ttaggtagag aattagaaaa
     1501 tattacagga catacgattg ctgattctac tggcgaaatt aagcgtgtaa ttgcacattt
     1561 accaaacttt gagtccgata cagatacttt tgttgctaca tatcgtttaa accatcaaca
     1621 agattttata gatgcaactt ttactgcgct gaaatcagat agagcacgtt taaaagaagt
     1681 gccagttcat gttgaactta taagttatat ttctaaatca aaataaactg ctatctaaaa
     1741 cgcaaagttg atcaaaatat cgattttgtg ttttttattg agaaattata taggagtgtc
     1801 aatcgatgat ttattgtgaa acagagcgtt taatattaag agactggcat gaagatgatc
     1861 tgttaccttt tcaaaaaatg aatgcgaatt atgacgtacg taaatatttt ccaagtttat
     1921 tgagttatcg tcgttcagaa ttagatatga gaactatgga tgcggttatt aaagattatg
     1981 gcattggatt atttgctgta gaagataaag agtcccatca atggataggc tttataggtt
     2041 tgaattatat tccagaaaca agcgattatc catttaaaga attaccgctt tatgaaatag
     2101 gttggcgctt gttgccagaa ttttggggaa aaggattagc aactgaaggc gcaaaggcaa
     2161 cattgaagtt agcagaagaa catcaaatat acgatgtcta tagttttaca gcagaagcaa
     2221 ataaagcttc acaacgtgta atggaaaaaa ttggcatgac agtgtatgat catttcgaat
     2281 tacccaatct aagtaagtat catttattaa aaaggcaagt gcgctattac attaatcttc
     2341 gaaagtgaaa aatttataca taagcgtaac aaacacccct aacattgttt agctgatgat
     2401 aacaatatta ggggtgtttg tttatttttt aaccttagaa tgattaatcg tatgaacgag
     2461 tacccagagg tttgaaattt aatattgatt caattaatga ttccttagtg tcgcataacg
     2521 gtgcaagagc acgatactta ggatcaataa aaccttcctc aatcatatgg tcaatcattg
     2581 tttgtagtgg attgaaaaag ccattaatat tataaatggc aataggcttt tcatggatac
     2641 ctatttgagc ccaactatac atttcgaaaa attcttctag tgaacctgcg ccaccaggag
     2701 ccatgacaaa tgcatctgca agttctgcca ttttattttt acgttcatgc atagaatcaa
     2761 ctaaaattaa ttcagttaaa cgttggcttg tgatttcatg ttcatctaac attttaggca
     2821 tgacgccaat agctttgccg ccatgatcta atacaccatc ttgaatggca cccataatgc
     2881 caattgaccc tgcaccaaat actaattcat aaccttgttc agcaaaatat ttacctaaat
     2941 cgtatgcttt ttgtacatat gaagggtcat gacctttgct tgcaccacaa taaactgcga
     3001 ttcgtttcat gttaatccag ctccttaatt cgatgaatga cttttaatag tgattgttca
     3061 aacacttttt gatcttgctt tgtaaaaggt gggggacctt tgtggcgacc accttgtttt
     3121 ctaatttgtg cattcatata tcgtttatct aatagttgtt gaatattttt ggaattgtat
     3181 atcttcccat tatgatgcat gacaattaag actttgtcga ctaataaact tgcgagtcca
     3241 taatcttgag tgactacgat atcatccttc gttgataatt gaacaatttt gtaatcaact
     3301 gcatctggtc catcatcaac atataatgtt gatacatgtg gaggatataa ttggttcgaa
     3361 aaatggctga agctccgaat aattgtcaca aaaatgcctg tctcagttgt taaatctata
     3421 atagaatcaa caacaggaca agcatctcca tcaataataa tatgtgtcac aattatgcct
     3481 ctgtattgtt ttctttattt tgttgagagg cgcttttggc aacataatct ttatattttt
     3541 taaatgactt gatgcgtgct ttatcagctt cttgttggcg tttttgttct tctttgtgtc
     3601 gtttttcaat atttttttgt aactttttat tcattttagc gatttctttg cgattttttt
     3661 cagctagttt atcgcctttt ttctcagttt tctcatctaa tttattaggt gttaagcctg
     3721 ctttttcttc gtatttttgt gattttttca tatctttaat acgttgtatt tcattctttt
     3781 cgcgggcttt ttgctcttct ttatgacgct tttcgatatt tttttgaagt attttattca
     3841 ttttatcagc gtctttacga ttttgtttag ctaatttttc gccttttttc tcaatatagg
     3901 caggatcatg ttctctagca aactttttaa gttcacgttt attttcaaaa tcttgttttt
     3961 tatcgccgac atattcttta acatcactcg ctgtgttact gattgctgca gatgtttttg
     4021 aagcaacttt acttgtagca tctgtaactt tttgtacgtc cggatgttgt ttgatacgtt
     4081 tacgttcaac aattaacggt accaatacaa ttggtaatac attaatcata aatttgatga
     4141 cttttttctt atccatagat cttgcctcca taattacttt attaatttta cataccctat
     4201 gatacatcaa tataaacgat gatagtagtg aatcactatt aagtatttca gatctttttt
     4261 aaaagaagac aataaaaact gccaatcaag tggttcctta attgacagtc tatattttaa
     4321 aaggaaatta aataccttta ccaatgccaa atccgaagta aagtatagca ataaagatta
     4381 ctaatacaat tctgtaaatg gcaaatggaa ttaatttgat tttgttaatt agatgcaaga
     4441 atgttttgat tgcaattagt ccaacagtaa atgcagctaa aaagcctaaa atataaaaag
     4501 gtatatcagt aatctgaata tcttgataat gttttaataa agataaacca ctagctgcta
     4561 acataattgg aacagccata ataaatgtga agtccgatgc tgctttatga tttaatttca
     4621 ttaatacccc agttgaaatt gttgagcctg aacggctgaa accaggccac atagctactg
     4681 cttgagagat accaattaca aacgcttgga aataattgat ttgatccact gtttgtgggt
     4741 ttttaacttt agctgagtat ttatcagcaa taatcatata gatagcacct acgaataagc
     4801 caatcataac agttggcaca ctaaataaat gttcttcgat gaaatcatca aatagtaagc
     4861 ctaaaatacc tgctggaacc atacccacta atacatgtaa taaatttaaa cgtcttggct
     4921 ttgaacgtct ttgttgattg ttatctcctt caacatgttt gtgtttacca atatgtaaaa
     4981 tctctaagaa gcgttcgcgg aacacccatg ctgctgcaaa gacggatcct aattggatga
     5041 cgattttaaa tgtaaatgct gattgagaac ctaaaaattc agatgatttt aaccacatat
     5101 catcaactag aatcatatgt ccagtagagg aaacaggtgc aaattctgtt aatccttcga
     5161 cgacccctaa gataatacct tttattaatt caatgataaa cataatgtac ccactttcat
     5221 tactcaattt aatttattta aatatcaaaa ttaccatatc atgatagcat attcatttaa
     5281 agacatgcta gttatagtta taatactaga ctaaagatgt atatattcat tttcttttac
     5341 atgtaaaact acaatatttt attgagctat ttaatttgat tttaaggaaa accttttata
     5401 ataggtttag gtgatataat tgtgaaaaaa ttaacaacaa tactgtttca atataaaatt
     5461 tttccggtac tcatgttctt ggtcagtact ggtctcggca tactcgttat aacgcaaaat
     5521 attttaatag cagatttttt agctaaaatt ataagacatc aatttcaagg tttatggatt
     5581 gtattattta ttttattagg tgttttactt ttaagagcaa ctgtgcaatt tctaaatcaa
     5641 tggttaggtg atacattagc atttaaagtt aagcatatgc ttagacagcg ggttatttat
     5701 aaaaataatg gtcatccaat cggtgaacaa atgactatac tcacagaaaa cattgatggt
     5761 ttagcacctt tttataagag ttatttgcct caagtgttca aatcaatgat ggttccgctc
     5821 atcataatca ttgcaatgtt tttcatccat ttcaataccg cattaattat gttaataact
     5881 gcaccattta ttcctttgtt ttatattatt ttcggtttga aaacgcgaga tgagtcaaaa
     5941 gatcaaatga cttatttgaa tcaatttagt caacggtttt taaatattgc taaaggttta
     6001 gtgacgttaa agctatttaa tcgtacagag caaacagaga agcatattta cgacgatagt
     6061 actcagttta gaactttaac aatgcgcatt ttacgcagtg cttttttatc gggattaatg
     6121 ctcgaattta taagtatgtt aggtattgga ttggttgcat tggaagcaac gctaagctta
     6181 gtagtatttc ataatattga ttttaaaact gcggcaattg cgattatttt agcgcctgaa
     6241 ttttataatg caattaagga cttagggcaa gcgttccata ctggaaaaca aagtgaaggt
     6301 gccagtgacg ttgtgtttga gtttttagaa caaccgaaca ataataatga atttctatta
     6361 aagtatgagg aaaatcaaaa gccatttatt cagttaacag acatatcatt tcgatatgat
     6421 aattctgata gattggtatt aaatgattta aatttggaaa tatataatgg tgatcaaatt
     6481 gcacttgtag gtccaagcgg ggcaggtaaa tccactttga cacatctaat tgcaggtgtt
     6541 tatcagccaa caataggtac tataagtaca aaccagcgtg atttaaatat aggaatactt
     6601 agtcaacagc catatatttt cagtgcttct ataaaagaga atattacgat gtttaaagat
     6661 atagaaaata atactattga agaagtgcta gacgaagtag gtttattaga caaagtgcaa
     6721 tctttcacaa aaggcattaa cacaataata ggtgaaggag gcgaaatgtt atctggtgga
     6781 cagatgagac gcatagaact ttgccgtctt ttagttatga agccagatct cgttatattt
     6841 gatgagcctg caactggttt agatattcaa acagaacaca tgattcagaa cgttctgttt
     6901 caacatttta aagatacaac gatgattgtc attgcacata gagataatac aattcgccat
     6961 ttacaacgac gcttgtatat agaaaatgga agactgattg ctgatgatcg caatatttca
     7021 gtaaatataa cagaaaatgg tgatgactta tgaaaacacg actaaaattt caagtagata
     7081 aggatttatt gttagctata gttgttggtg tttgtggaag tttagttgcg ctcgccatgt
     7141 ttttcttaag tggttatatg gtgacacaaa gtgcacttgg tgcgccacta tacgctctga
     7201 tgattttagt cgttacagta aaattgtttg ggtttttaag agctattact cgatacgtag
     7261 agcgccttat ttctcataaa gctacattta caatgctacg tgatattcgg gtacagtttt
     7321 tcggtaaatt agtaaatgtc attcctaatg tttaccgtaa actgagttct agtgatttaa
     7381 tttcacgtat gattagtcgt gttgaggcat tacaaaatat atatttacgt gtttattatc
     7441 caccagtcgt catcggtttg acagcgctag ttacagtcat agttttggcg ttcatttcaa
     7501 tcggccatgc gctattgatt atggttagca tgttgtttac tttactcgtt gttccttggt
     7561 taagctcaaa aaaagcacgt actttaaaga aacatgcagc taatgaacag gcccgatttt
     7621 taaatcattt ttatgattat aaagctggta tggatgaact acgtcgattt aatcaaatta
     7681 atcattatcg agataatttg atggctaaat taaatcattt tgataaatta caacttaaag
     7741 agcaacgctt tttaacgatt tatgatttta tattaaatat tattgctatg ctttcgattt
     7801 ttggtagttt agttctagga ttaattcaaa ttaatgcagg ccaactaaat attatttata
     7861 tgacgagtat agttttaatg gtcttaactt tatttgaaca agctgtacca atgacaaatg
     7921 tcgcgtatta taaagcggat actgaccaag cattgcatga tattaatgaa gtgatatctg
     7981 taccttctac taatggaaaa aaccgtctta atgataagta tgatgcaacg aacatttatg
     8041 aagttaagga cgctagtttt aagtattgga accagcaaac gtatgtgttg tcggatatta
     8101 attttaatgt taatagaggc gaaaagattg cgattgtggg gccttctggt tcaggaaaaa
     8161 gtacattact acaaattatg gctgggttat atcaattaga tagtggctct attcgtttcg
     8221 aaaatatgga tatgtttgaa atagatgaca aagataagtt tgaatcgtta aatgtcttgc
     8281 tacaatctca acaattattt gatggtacaa tacgtcaaaa tttatttacc gatgaaaaag
     8341 atgaagcggt gcaagcaata tttaagcaat tagatttaga acatttggca ctagaacgtc
     8401 aaattgactt agatggtcat acattatctg gcggagaaat tcagcgttta gcgattgcaa
     8461 ggatgttatt aaaagatact gcatcaacat ggattttaga tgaaccaaca actgcattag
     8521 ataaacaaaa tagtttaaaa gttatggatt taattgaagc acatgcagaa acattaattg
     8581 ttgctacaca cgatttaact ttattgtcac gttttgagac catcattgtg atgataaatg
     8641 gtaaaatagt tgaaaaggga aactatcaac aattactcgc taatcaaggt gctttatgga
     8701 atatgattca atataatgca taaaaaaact gcttgatagg ctaagaaacc tttcaagcag
     8761 ttttttaact aacaagctta cgcgtgctaa ttgtttttct attcttaata aattctaaac
     8821 attactttaa ttgtcatgac aaaagttaat tatttttcct ttgtttcatc aaatgcatga
     8881 atgactttac ctaataagcg attaagttct ttaacttcat cttgagataa agaagaagct
     8941 gaagcgactt tgtcagatgc attacttaat tctggtctaa tagtttcact tttgtcagtc
     9001 aagtgaataa atacttcacg ttgatcgact tcggaacgtt cacgcttaat taagtctact
     9061 tgttccattc gttttaataa tggtgatact gtaccagtat cgagtgctaa ttcagttacg
     9121 actttcttga cgtttacagg agattcatcc cataaaattg ttaagacaag aaattgtggg
     9181 tatgttagat tgtacttctt aaaaactttg ttagagtagt agcgattaac ttgtctttga
     9241 gcattgtaca aactaaagca tagctgttct tttaaattat gttgatcaga cattaaagtt
     9301 ctcctccaga catactatcc gtttttttct cttttcggat tggtaatcat taaaaagttg
     9361 attgtttatt aattcacaac tttctttgat tcaatgccat gctaaaatta aagtatgttt
     9421 aaagtttaga agata
//
LOCUS       DBNENW010000102         9236 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNENW010000102 DBNENW010000000
VERSION     DBNENW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.103"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            10..561
                     /locus_tag="AC1LW8_001922"
     CDS             10..561
                     /locus_tag="AC1LW8_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIP4773735.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLKVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPFDIL
                     NDSQFIEELANINLALKKGDIYD"
     gene            554..1774
                     /locus_tag="AC1LW8_001923"
     CDS             554..1774
                     /locus_tag="AC1LW8_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4773736.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(1846..2142)
                     /locus_tag="AC1LW8_001924"
     CDS             complement(1846..2142)
                     /locus_tag="AC1LW8_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIP4773737.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(2191..2394)
                     /locus_tag="AC1LW8_001925"
     CDS             complement(2191..2394)
                     /locus_tag="AC1LW8_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6007 family protein"
                     /protein_id="HIP4773738.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            2703..3689
                     /locus_tag="AC1LW8_001926"
     CDS             2703..3689
                     /locus_tag="AC1LW8_001926"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIP4773739.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            3853..4088
                     /locus_tag="AC1LW8_001927"
                     /pseudo
     CDS             3853..4088
                     /locus_tag="AC1LW8_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282781.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(4355..5548)
                     /gene="sqr"
                     /locus_tag="AC1LW8_001928"
     CDS             complement(4355..5548)
                     /gene="sqr"
                     /locus_tag="AC1LW8_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HIP4773740.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(5567..6901)
                     /gene="cstB"
                     /locus_tag="AC1LW8_001929"
     CDS             complement(5567..6901)
                     /gene="cstB"
                     /locus_tag="AC1LW8_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HIP4773741.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(6932..7999)
                     /gene="cstA"
                     /locus_tag="AC1LW8_001930"
     CDS             complement(6932..7999)
                     /gene="cstA"
                     /locus_tag="AC1LW8_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HIP4773742.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            8136..8396
                     /gene="cstR"
                     /locus_tag="AC1LW8_001931"
     CDS             8136..8396
                     /gene="cstR"
                     /locus_tag="AC1LW8_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HIP4773743.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            8396..9151
                     /locus_tag="AC1LW8_001932"
     CDS             8396..9151
                     /locus_tag="AC1LW8_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIP4773744.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIIQLIL"
BASE COUNT         3009 a         1547 c         1398 g         3282 t
ORIGIN      
        1 gttatgacta tgattacttg tttatttgga agtagtagaa aagatggcaa ttcagctatc
       61 gcagtagaga atctattgaa gaatttaaaa gtcaactttg tgaatttata tcaaagcaac
      121 attgaaaagg ttacagataa tcgtcatagc aaaggtgcca actttcataa tgatgattac
      181 gaacaaattt taatcaaagt tttagaatca gatattatta ttttttcaac accactctat
      241 tggtactcca tgagtgcgtc tttaaaactc ttcattgata gatggaccga gtcattgcga
      301 gatacgcaaa tagacaactt caaagaaatc atgtcacaaa aaaagtattt gattttaatc
      361 ataggtggcg acaatccgca taccaaagca caacctttag tcaaccaatt caaattaata
      421 tttgagttta tgaatattac taactataga tttttgatcg gcgaaggcaa taaaccattc
      481 gatattttaa acgactctca atttatagaa gagctcgcaa atataaatct tgcattgaaa
      541 aaaggtgata tttatgactg atttgttgaa aattaaaaac ttcagattgt tctttttagc
      601 agaaataatt tcagcattcg gagttggaat tagtacagtt ggggctaatt ggtatttaat
      661 cgacaaaact aacgactccc aactattagg tattatgtta gctttaaatg tattatccgg
      721 tttcttagct tctcctataa taggtggact agccgataaa tataatagac gaaatattat
      781 tttaatcacc tatcttcttc aggtaatatt atatttactc attgtcatag cactcgtaat
      841 gataggattt gagacctatt tagtgattgg atttgcgatt gttaatggga taggttggac
      901 tacgtatatg gcaacatcta gaagtttagt taaacagata ttaaaaccag atcaatatac
      961 agatgcaaat tctctattag agattagttt gcaaacaggt atgtttattg caggtggatt
     1021 atcaggaata ttatataaaa taaatggatt cactctaatt atagcgatga ctataatgat
     1081 gtttctaatc agcattttta tgttatttag attgcatgta gataaaccaa ctcattcaga
     1141 ggaagaatca acaaatagct tattgcaaga atatttatta ggatggaaat ttttaaaaga
     1201 caacatgatg atatttattt ttggagttat ttcaatcata ccaatggtgt ttacaatgat
     1261 ctttaacata tcattaccag gatatgttta taatgtttta aaactttcgt ctgtacaatt
     1321 tggtttttca gatatgttat atggcattgg aggattatgt gcaggtttaa tttcagctat
     1381 tctttcgaag aaaatctcaa ctaaagtatt gatatttttg ttatatttca tattagtcat
     1441 aaattcagca ctatttattt ggataaactc agcattttac ttattcatag gatcatttat
     1501 actaggctac tcaatttcat caatcagaat ttatatgaat acagctataa tgaacactgt
     1561 ttcagataaa tatgtcggtc gctcatttac gatatggacg tcaatttcat tgttactgca
     1621 aagtttcatt gctccatttt taggaagatg gattaatgaa attaatgata aattcggttt
     1681 ctatattata ctcattttat ccttactcat atttgttaca ctgctgcttg ttaacaaaac
     1741 agacaaaata aaatatgcac ataaagaaga gtgaccgtca ctcttcttta acaagcgacc
     1801 atttatcgat gggcttagtt ctctctgcac ccacacttca ctacttcact ttttcaaatc
     1861 attttttatg gtcttaaata aatcagtgag atttgttgct tcggtaaagt ctagaattaa
     1921 tatcatttct ttagaacctg gatatggcga aactaatgta ttatcttgca atttctgctg
     1981 ggcactttta gtcgccttga ccaataatct attatcatac aaaccaccta taaccacgcc
     2041 atcataataa ataatatatt ctcccatcat ctttcttgtc ttaaccgcgt ttgaattcac
     2101 atgatttaaa aataaatcat gtacatcttt cttagtcgcc attgtaatcg ctccttcagt
     2161 tttatgttta atcacattcg tattaatgat tcatttcgtg ttgctcttaa ttttatctat
     2221 aattatatgc gtagttaaaa tcaaacctat ggaaaagaaa ataatgataa caatgttaag
     2281 aaatatagtt ataaaattat agtttggaag gtatgcgaat agcagaaaaa taggtatcgc
     2341 aaaaaataaa tcccaccaac ctaaactttt taaagaatgc tttaaacctt ccataatatc
     2401 acctttataa atttgtcttt gttataagat aactaaaaaa tcgctttact gtaaaagtag
     2461 ccaaagaaaa ttctgaatca tattcataag tagtgtatca ttaataatga acaatttaat
     2521 actataatcc ttgatctttg tattgatcaa cttaccacaa catttatttt agactactct
     2581 tagacttccc tttcaaatgg ttgcatctat tgaaattcct tttgtataag ttaggctttt
     2641 gtggtaatat catcatgcat aaaaaatcga gatactaatt ataaagaggg tataaatata
     2701 ttatgaaaga aaatttttgg agtgaattac cacgtccatt ttttattttg gcgccaatgg
     2761 aagacgttac agatatcgtc tttcgacacg ttgtaagtga agcagctaga ccggatgtgt
     2821 ttttcactga atttacaaat actgaaagct tttgccaccc tgaaggcata catagtgtgc
     2881 gcggacgctt aacttttagt gaagatgaac agccgatggt cgctcatata tggggagata
     2941 agccagaaca gttccgtgaa acgagtattc aattagctaa aatgggcttt aaaggcatag
     3001 acttaaatat gggatgtcct gtagcaaatg ttgctaaaaa gggtaagggt tccggcttaa
     3061 tcttaagacc tgacgttgct gccgaaatta ttcaagcgac taaagcaggt gggcttccgg
     3121 taagtgttaa aacacgcctt ggctactatg aaatcgatga atggaaagat tggttgaagc
     3181 acgtcttcga acaagacatt gccaatttat ctattcatct tcgtacacgt aaagaaatga
     3241 gtaaagtaga tgcacattgg gaattaatcg aagctattaa aaatttacgt gacgaaattg
     3301 caccaaatac attgttaaca attaacggtg atattcccga tagaaaaaca ggacttgaac
     3361 tggcagaaaa atatggcatt gatggcgtca tgattggtag aggcattttc cacaatccat
     3421 tcgcttttga aaaagaacca cgtgaacaca caagcaaaga actattagat ctgttgagat
     3481 tgcatctatc attgtttaac aaatatgaga aagatgaaat acgacaattc aagagcttgc
     3541 gtagattctt taaaatctat gtgcgtggca taagaggcgc tagcgaactt cgccatcaat
     3601 tgatgaacac acaatcaatt gcagaagcaa gagcacttct agatgaattt gaagcccaaa
     3661 tggacgaaga cgttaaaatt gaattatagt atgagtaata aagtttatgg atgatatttc
     3721 ccaatttaac acggattgaa cactttctac ttaggtatta tcttggtttt cctgatagga
     3781 taactcccga tgatgccttt ttacttatgt cacttatcct tttggaaatg gtgcaaaatt
     3841 aaaaatacat caggatatcg tttaactcag aaaaaaactc caatcaaaaa aatgtttaca
     3901 acacattatg aaattaaaat tgctcttagt gttcaacaga caaagttatt gaaattacta
     3961 ttggaggtac ataccaagct atcattttga gtacacgtgg tttgaatatc tggcaagaaa
     4021 tggaaattgg taatgtcagt cataaagtcg ctaaacgtgc tcaaatacca ttaattattt
     4081 ttaaataatt tagaggatga ggtataaacc tttaaaaaac agcagtgaga tgattttcaa
     4141 ttagaaaata tcttactgct gtttctattt tcataatcat tcttattgaa tgacttcatc
     4201 gtgatcattt aaaacagtaa caactttttc aatcttatac tatttagagc taaaaattca
     4261 cgacatataa acacaacact gacacgtcat ttaaatactt aatcttttat agtaaaagat
     4321 ttttcattaa aaatttattg ttttctgtac tttattatat taatcctttt agcatgccgt
     4381 accaatacat tttaggtaat aaatctttct taaatatata catacttcta cgttctttgg
     4441 cctgattaaa cggcattgtt tctttagtat ttttattata atcaaactct gcaagtatta
     4501 acctattata tccagtaaca atagggcatg aagtataacc atcataatga tgcgttaaca
     4561 tttgattatt catcacttgc aataaattat tagcgacgat aggtgcttgc ttacgaatag
     4621 ctgcgcctgt ttttgaagta ggtacatttg aagcatcacc aagtgcaaat acattagagt
     4681 agcttttatg ctgtaatgtg gttgggttaa catctaccca accctcacta tctgaaagtg
     4741 tactttcttt tactacatct aagggaccca taggtggtgt tacatgtaac atatcataac
     4801 ttattgtttt tctatcgtat gctttgatat gttcgaatgt agccactttt ttgtcaccgt
     4861 cgatttcaac aaggttataa ttataattga ctgttatatt tctttcttca acaatccttt
     4921 ctaattcttt attatatttt cctacgtcaa ataaagcatc ttttggcgtt gcatatatca
     4981 cattagcgtt agaacggatt ttatgtttcc taaaataatc ttcagctaaa tacataattt
     5041 tcataggcgc acctccacac tttataggag tgtttggatg cgtaaaaatg gcatttcctt
     5101 gtttaaaatt agaaatttgg ttccaagttt cgttaacata gtcaggtgaa tagttagagc
     5161 aaacaccatt tttacctata ttttctttta gtcctttaat tgaagaccaa ttaatctgta
     5221 atcctggagc tactactaaa aaatcataat aaacgactgt attatctcct aaaataacgc
     5281 tattattttc aggttgaaaa cttgaaacag cctgttttat ccagttagca ccttcaggta
     5341 taacactttc catatcttta cgagaacttt tcaaactaga tacccctgca ccaaccaacg
     5401 tccataatgg ttgatagtaa tgatggtctg ctggatctat aattgctatt ttctctttta
     5461 agttttgatt ttttcttaat agtcttgatg ctacggtaac acctgctgta ccgccaccaa
     5521 taataacaat ttgataatgc ttattcattt tttccacatc cttaaattat tttaatgatt
     5581 ctggaaaatc ttgatagcct tctctaatat tcaccacatt ttcaaaacct ttgctttcca
     5641 atatacccac tgcaattgaa cttctaacac ctgactgaca atgtacatat attttatcct
     5701 ctttattaaa aggaatattt tcatttaata atttaccatg cggaatatta actgcttgat
     5761 ctaagtgtcc attattccac tcttcatcat tacgtacgtc taatacatgt tcttctttac
     5821 ctgtcatatc agcgctatga acggactggg ttgaaatgcc tgattttggc aaacgatagc
     5881 ctgctacctt atcaaaccca attaattgta aagtgtgttt cgctttctca acagtagatt
     5941 tatctccaat taaatctata tctttttcaa aatctaagta ccaaccaatt tgattaataa
     6001 agtttttgtt gtatggtata ttgattgttc cttttgtgtg gccaccgtga aaggcctctt
     6061 tgctacgaag atcaaatgct actctcttat tatctaaact aggataaaca tcatatgatt
     6121 gatataaatt cataccaaac tgattaactt gtttcatttg tgcaaaatga tgcggtggtg
     6181 ctggttgatt tgatgttaat gattcaataa atttagtctc atcaatttca ttaaatgccc
     6241 agttattaat tttctcataa cctattgtag atataggtat ggcacctaat gctttaccac
     6301 aagggcttcc agcaccatga cccggccaga tttgaacata gtctggtaaa tttttaatat
     6361 tttgaacgga ctcatacatt tgtttcgcgc taatttctgt agaacccttt atttgaacag
     6421 atttttctaa taaatcaggt ctacctatat caccaacaaa aataaagtca ccactaaata
     6481 atcccatcgg aacacttgaa ccaccaccta aatcagtgag taaaaagcta atactttcag
     6541 gcgtgtgtcc tggagtatgc agaacttcta atttaacatt gcctacttga atgatatctc
     6601 catgtttaac aaattgtgtt tttgatggca tatttttata ccctaatgca tcttcacctt
     6661 cgccagacac atatatattt gcatttaagc gtttagccac atcacgaatt cctgaagcaa
     6721 aatcagcatg aatatgtgtt tctgtagctt gtgtaattgt taaaccttca gaatctgcaa
     6781 cttctatata tttcgataaa tcacgaacag ggtctattat tattgcctct cctgtacgtt
     6841 gacaacccac taaatatgat gcttgagata aatgattatc gtaaaactgt ttaaaaaaca
     6901 tgataacaac tccttttatt aatagattta attagataaa taaattatga ttcgcatttt
     6961 cagtagcacc aatataagtg cctacaccac cgtactcaac ttcatctcta agttcttctt
     7021 tctgaatacc catgacatcc atactcatcg tacaagcgat taatttgata ttttgctcaa
     7081 tagcttgatt gataagtgtt ggtaatgaat caacattttt ctttttcatt acgtagcgca
     7141 tcatcatatt tcctaaacca aacatattca ttttggaaag aggcattcgt atattctttt
     7201 tgggcaacat taaatcaaac atttttgcaa taccttgctt tttaacatta actgtttgca
     7261 ctttttttaa tgcattaagc ccccaaaaag taaagaagat agttacatct tttccagcag
     7321 ctctagcacc atttgcaata atcaacgctg ctacagcttt gtctaattct ccactaaata
     7381 atacaattgt agtaccttta gcagaataat ttatatctaa atcttttgct ttttcttttt
     7441 gaataatcgc attaattcca ttgttatttt catcaagctt aactaaagta tgccttgttt
     7501 gtttcaccca acttttaatg tcactaggga atccagggtc tgtgactttg acttcaattt
     7561 ggtcacctac ttcaatattc ttcatttctt gactaatttt tacaattggc cctggacatt
     7621 gaagaccacg atagttaaat tgtttacgat ctgctttaat ttctatactt ttatattctt
     7681 cttgtgtatc ataactatcg ttttcttctt cataagcttt ataaccacca tcaagattta
     7741 taacgttata accttgttta gctaaatatt tactagcatt agcacttctg ttaccacttc
     7801 tacagattac ataataggtt ttattttttt ctttattgaa tgactcaatc tcatcaacag
     7861 gatgaagtat ggaaccattg atatgtccta atgcatactc ctcttctgtt ctaacgtcaa
     7921 ttaattgccc ttgcttggct agtttttcca attctgcttt actaaattca tcgataaact
     7981 tttctccgta ttgtttcata ataacctccc taaaaatacc tgtaggggta ttttaaacag
     8041 ttggatggaa cttgtcaaat acctctaggg gtatttgaca catttgtgtt tatctattaa
     8101 tatattttta ttcaattaaa agtggaggta tgactatgaa ttatgataaa aaaatgatta
     8161 atcgtattaa tagaatacaa gggcaactaa atggaattat taaaatgatg gaggaaggaa
     8221 aagactgtaa agatgtcatt acacaaataa gtgcatcaaa gagttcactc caacgcttga
     8281 tgggtatcat tattagtgag aatttaatag aatgtgtaaa agcagctgcg gatgatgaag
     8341 aaagctccca agagttaatt aatgaagctg taaacttatt ggtgaaaagt aagtaatgga
     8401 tattgttaat atagttatca tgttactcat tggggtattc ggtgggttca tatctggtct
     8461 agtaggcgta ggtggtgcta tcattattta tccagctatt ttattattac caccattatt
     8521 cggagcccct gcatatagcg catatatcgc atcaggtctt acttctagcc aagtcttttt
     8581 cagtacatta agtggctcat taaaagctcg aaaaaaaaca gagttctcgc cacaattagt
     8641 tctttatatg ggtggcggca tgattatagg gagtatgtta ggtgcatttc tagcaaattt
     8701 atttgatgct acatttgtaa atacggtata tatcattatt gctttacttg cattaacatt
     8761 aatgtttatt aaagtgaaac ctagttccga aaagtcttca tttaataaat atttattagt
     8821 tatcattggc cttttcattg gtattatttc aggtatagta ggtgcaggtg gtgccttcat
     8881 tattattcct atattattgg ttttattcaa attaccaatg aatactgtag tcgctaatag
     8941 tatagtaatt gcttttatat cttcaattgg tgcttttgtc atcaaactta ttcaaggtta
     9001 tatcccttta tatgacgcct tattccttat aatagggagt attatatttg ctccaatagg
     9061 gttaaaatta agtaaaaaag tccctaacat aatacaaaaa tggattatca gtattttaat
     9121 cgtttttgct ataattcaac tcattttata aaactaatcc ttttaactca gacagcaatt
     9181 tcccgaaaaa taatacctct tatttaaaca aatatgtatg cagttcggat ttatat
//
LOCUS       DBNENW010000103         9188 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNENW010000103 DBNENW010000000
VERSION     DBNENW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.104"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..125
                     /locus_tag="AC1LW8_001933"
     CDS             <1..125
                     /locus_tag="AC1LW8_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773745.1"
                     /translation="SIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGKTHES"
     gene            115..639
                     /locus_tag="AC1LW8_001934"
     CDS             115..639
                     /locus_tag="AC1LW8_001934"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIP4773746.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      626..723
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            798..1889
                     /gene="gcvT"
                     /locus_tag="AC1LW8_001935"
     CDS             798..1889
                     /gene="gcvT"
                     /locus_tag="AC1LW8_001935"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIP4773747.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1909..3255
                     /gene="gcvPA"
                     /locus_tag="AC1LW8_001936"
     CDS             1909..3255
                     /gene="gcvPA"
                     /locus_tag="AC1LW8_001936"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIP4773748.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQLFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3248..4720
                     /gene="gcvPB"
                     /locus_tag="AC1LW8_001937"
     CDS             3248..4720
                     /gene="gcvPB"
                     /locus_tag="AC1LW8_001937"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIP4773749.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5085..5471)
                     /locus_tag="AC1LW8_001938"
     CDS             complement(5085..5471)
                     /locus_tag="AC1LW8_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP4773750.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5629..6459
                     /locus_tag="AC1LW8_001939"
     CDS             5629..6459
                     /locus_tag="AC1LW8_001939"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIP4773751.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFNTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDKHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6523..6741)
                     /locus_tag="AC1LW8_001940"
     CDS             complement(6523..6741)
                     /locus_tag="AC1LW8_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA1362 family protein"
                     /protein_id="HIP4773752.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6755..7336)
                     /locus_tag="AC1LW8_001941"
     CDS             complement(6755..7336)
                     /locus_tag="AC1LW8_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773753.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7441..8502
                     /locus_tag="AC1LW8_001942"
     CDS             7441..8502
                     /locus_tag="AC1LW8_001942"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP4773754.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDAEEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8528..9085
                     /gene="efp"
                     /locus_tag="AC1LW8_001943"
     CDS             8528..9085
                     /gene="efp"
                     /locus_tag="AC1LW8_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIP4773755.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3275 a         1246 c         1686 g         2981 t
ORIGIN      
        1 tttccatttt cactttttat atcagtcaat atagtttaaa attaaagaca ttatataata
       61 ttgaaactga ctataactat aagattgttt tgttattgaa agaagggaaa acgcatgaat
      121 catgataaat caccaataat tttaattggt ttcatgggta ccggtaaatc tacgattggt
      181 aaatacgttg cagatgagca aaatttatca tttattgata tagattcata tatcgaagag
      241 aagtataagt taacaatacc agaaatattt agtaaacatg gtgaacaata tttcaggaat
      301 ttagagttca catgtttgca agaatgtatt aacactgcag atataattgc tactggtggt
      361 ggtattattg agagtgaaga ggcatttaat tttttgaaaa atcaaaaaaa cattatttgg
      421 ttagattgta atattgatat tatatatagt cgaatcaatg atgacccaca tcgacctaat
      481 gcaaataata agacaatcaa gcagttaaat gacttgtatt gctcgcggaa tttaagatat
      541 aatgaaatcg cattcaagaa atttgatagt catttgctat caatttcaga aatatattat
      601 gaattgctaa atttaataaa agcgagtgat cagtattaga gagaatagag cgttaagact
      661 ctatcgccga aggtgcaagt aatttattac gaaactctca ggcaaaagga taatactgta
      721 acgcgttcct gaattggtga tttataaaca gggtagcgat tgctatcctg tttttataat
      781 tttaaggggg tatttcaatg tcaagtgatt taaaacaaac acctttatat caaaattatg
      841 ttgatagagg tgcaaaaatt gtggaattcg gaggatgggc gatgcctgtt caattttcaa
      901 gtattaaaga ggagcataat gctgttcgat acgaaattgg cctgtttgat gttagtcata
      961 tgggtgaaat tgaagtaaca ggtaaagatg ctagtcagtt tgtgcaatat ttattatcaa
     1021 atgatactga taatttaact acttcaaaag cattatatac tgctttatgt aatgaagaag
     1081 gcggtattat tgatgattta gtaatatata aattagctga cgacaattat ttattagttg
     1141 ttaatgctgc taatactgaa aaagatttta attggatttt aaaacacaaa gagaaatttg
     1201 atgttgaagt acaaaatgta tcaaaccaat atggtcaatt agcaatacaa ggaccaaaag
     1261 ctagagattt aattaatcaa ttagttgatg aagatgtaac tgaaatgaaa atgtttgaat
     1321 ttaaacaggg tgtcaaatta tttggagcaa acgtcatttt atctcagtca ggttacacag
     1381 gtgaagatgg gtttgaaatt tattgtaata tcgatgatac tgaaaaaatt tgggatggtt
     1441 tattagagta taatgttatg ccatgtgggt taggcgctcg tgatacatta agattagagg
     1501 ctggattgcc attacatgga caagatttaa ctgaatcaat tacaccatat gaaggtggta
     1561 tcgcttttgc aagtaaacca ttaattgatg ctgattttat tggtaaatct gtattaaaag
     1621 atcaaaaaga aaatggtgca ccaagaagaa cagtgggatt agaattactt gaaaaaggaa
     1681 ttgcaagaac tggttatgaa gttatggatt tagatggaaa tattattgga gaagtaactt
     1741 caggaacaca gtctccatca tcaggaaaat caattgcact tgcaatgata aaaagagatg
     1801 agtttgaaat gggtagagag ttgcttgttc aagttcgtaa gcgtcaatta aaagcgaaaa
     1861 ttgttaagaa aaatcaaatt gataaataat taaaaagggg tgtgcattgt gagtcatcgt
     1921 tatatacctt taactgaaaa agacaagcaa gaaatgttac aaacaattgg tgcaaaatct
     1981 ataggagaat tattcggtga tgtaccaagt gacattttat taaatagaga tttaaatatt
     2041 gctgaaggcg aagcggaaac aacgttactt agaagattaa atcgcattgc aagcaagaat
     2101 atcactaaag aaacgcatac atcatttttg ggtgctggtg tttatgatca ttacgcgcca
     2161 tcagttgtgg atgctatgat ttcaagatca gaattttaca cagcatatac gccatatcaa
     2221 ccggaaattt ctcaaggtga attacaagcc atttttgagt tccaaacttt aatttgtgaa
     2281 ttaacagata tggatgtagc aaattcttct atgtatgatg ggatgactag ctttgctgaa
     2341 gcatgtatct tagcatttag tcaaacaaag aaaaataaaa ttgtagtctc aaaaggttta
     2401 cattatcaag cattacaagt acttcatact tatgctaaga cgcgtaagga attcgaagta
     2461 gtggaaatcg atttagatgg cactgttaca gatttgaaaa aattagaagc agcagtggat
     2521 gatgaaacag cagctgttgc agtacaatat ccaaacttct acggttcaat tgaagatctt
     2581 gaaaagattc atagctttat tgaagataaa aaagcattat ttatcgtata tgcaaatcca
     2641 ttagcattgg gattacttac acctccaggt tcatttggtg cagatattgt tgtaggtgat
     2701 actcagctat tcggaattcc tgctcaattc ggtgggccac attgtggata ttttgcaacg
     2761 acgaaaaagt taatgcgtaa agtgccaggt agattagtcg gtcaaacaca agatgatgaa
     2821 ggtaatcgtg gctttgtatt aacattacaa gcacgtgaac aacatattcg acgtgataaa
     2881 gcgacatcta atatttgttc taaccaagca ttaaatgctc tagcgtcatc aattgcaatg
     2941 tcagcacttg gtaaacaagg tatctatgat attgcagttc aaaatataga acatgctaac
     3001 tatgctaaac agcaatttat taaaaaaggt tttgaagtat tagatggtac atctttcaat
     3061 gagtttgtag tcaaatttga caaaccaatt caacaagtta acgaggaatt agtgaagtac
     3121 aatattatcg gtggttttga tttaggtgtt gtttcagacg actttaaaaa tcacatgtta
     3181 atagctgtaa ctgaattaag aacaaaagat gaaatcgata catttgtaga aaaggctggt
     3241 gagttaaatg actagtaaat caagtccatt aatttttgaa agatctcgtg aaggcagata
     3301 tgcatattca ttaccaaaaa gtgatattaa aacaaattct gttgagtcat tgttagatga
     3361 taaatttatt cgtaaaaaca aagcggagtt tcctgaagtt gctgaactag acttagtacg
     3421 tcattatact gaactttcaa ataaaaattt cggtgtagac aacggatttt atccattagg
     3481 ttcatgtacg atgaagtata accctaaaat taatgaaaag gttgctagaa taccaggatt
     3541 tagtgaatca catccattac aagatgaaga ccaagttcaa ggttcattag aaattattta
     3601 tagtttgcaa gaagaattaa aagaaattac tggtatggat gaggtgacat tacaaccagc
     3661 tgctggcgca catggtgaat ggactgcatt gatgatattt aaagcttatc atgagaataa
     3721 tggtgaaggt catcgtgatg aagtcattgt gccagattct gcgcatggta cgaatccagc
     3781 ctcagcttca tttgcaggat ttaaatcagt tactgtaaaa tcaaacgaac gtggcgaagt
     3841 tgatattgat gacttgaaac gtgttgtaaa tgaaaataca gcagctatta tgttaactaa
     3901 tccaaacact ttaggtattt tcgaaaaaaa tattatggaa atccgtgaaa tcgtccataa
     3961 tgctgggggt ctattatatt atgatggtgc gaatttaaac gctattatgg acaaagttcg
     4021 cccaggagat atgggatttg atgctgttca tttaaacttg cataaaacat ttactggtcc
     4081 acatggtggt ggcggtcctg gttcaggtcc agtcggtgta gtaaaagaac tagcaagtta
     4141 cttaccaaag ccaatggtta ttaaagatgg cgacaaattt aaatatgata atgacattaa
     4201 aaattctatc ggacgtgtaa aaccatttta tggtaacttt ggtatttact taagagctta
     4261 tacgtatatt cgaactatgg gagcaactgg acttaaagag gtttctgaag cagcggttct
     4321 taatgcgaat tatattaaag cacgtttatc taaacacttt gaaatacctt ataaacaata
     4381 ttgtaaacac gagtttgtgt taagtggtgt gcgtcaaaaa gaatttggtg tacgtacttt
     4441 agacatggct aagcgattat tagatttcgg tgtacatcca ccaacaatat acttcccatt
     4501 aaatgttgaa gaaggtatga tgattgaacc gactgagaca gagtctaaag aaacacttga
     4561 ttattttatc gatacattaa ttagtattgc tgaagaagct aaaaatgatc ctgataaagt
     4621 gctagaagca ccacatacaa ctgtgattga tcgattagac gaagctacag ctgctcgtaa
     4681 accaatatta aagtttgaaa atcttaaaca ggaaaaataa agtattaaac acatattccg
     4741 agaattatta ttctaacttt gtatgaagat ttaaggataa tggtttcaaa atcaattgaa
     4801 aaagacaatt tctatttaaa caagaaaact aaaccgaagt aataactctt agggtttggt
     4861 attattcttt catagaaatt gtcttttcat tttttagatt gcggtaattg aatcgtattg
     4921 aaaatgagct gaactttctt tattatgctg aaactaagtt taatgatcag tgaagctaga
     4981 gaaatatagt aacagacttt gttaaaaaat taaaatctga gtattgttat tgatgatcaa
     5041 ttaacactca gattttatta aatcgtatat taaatttaca aaaactattt tttagacttt
     5101 atttttccag tccatttttt atagccgcct tttaacatat agatatctgt atatccattc
     5161 tttttcaaaa tacgagcggc tctatagcta gcaatcccat tggcatcaca taagtatacc
     5221 ggttggtctt ttcttaatcc ttggaatcgt tgcctgaaca ttgtcatagg aatattgcga
     5281 gacccattaa tgtgaccgta gtcatagtca actttctctc taacatcgat gacttgagct
     5341 tttctaatcc cattatggaa ttcattttga tctaattctt taacagctcg cttgttaaga
     5401 atttgttgaa caatcatata agcaataatt gctataacta aaattattgc gatgtacaaa
     5461 ctagcactca tcttgcatcc tccctaatta aacgataata taattataag actgttaggt
     5521 caaattatca aaatatttca ctaaagaatt acaattatta ttcatttagt taaattgatt
     5581 aattagcgaa tcaaatatta tacattttgc tgataggagt tcttgaatat gactgaaact
     5641 tggaatttta ttaatacagg aagtaaagac ccatattata atatggcaat ggatgaagcg
     5701 ttattaaatt ttgtctctag aggtgaaatt gatccagtta tacgttttta cacatggaat
     5761 cctgcaacat tatcaatagg gtatttccaa aggctacaaa aagaaattga tattgataag
     5821 gttaaagaga aaggttttgg tctcgtaagg cgtcaaactg gtggccgtgg ggtgctacat
     5881 gataaagagt taacttacag tgtgattgtg cctgaatcgc atccgaatat gccttctaca
     5941 gttacagaag cgtacagagt gatttcacaa ggattattag aaggatttaa aaacttagga
     6001 tttaatactt attttgcggt acctaaaacg cctgaagagc gtcaaaaatt aaaacaacct
     6061 cgaagttcag tgtgttttga tgcaccaagt tggtatgagc tagttgttga aggccgtaag
     6121 attgctggga gtgctcaaac gagacaaaag ggcgtaatcc tccaacacgg ttcgatttta
     6181 caagatattg atattgatga actgtttgat atgtttattt ataaaaatga aagattaaaa
     6241 ttaaaaatga aagaggcatt cgttgaaaaa gcagttgcca tcaatgatat ttcagataaa
     6301 cacattacta ttagtcaaat ggaagaagct ttcgagaaag ggtttaagaa aggtttaaat
     6361 atagaattaa agccacttga attaacagaa gctcaattgg cagaagtgga agaattaact
     6421 gaaaaatatc gttcagatga atggatgttt agaaagtagt ttttagaata aaaaatggaa
     6481 gcacgtagac tattttacct acaagtgctt ccattttttt atctattttc ttctttgatt
     6541 tcttttatac ttacgcatcg ctttgcgata tttgcgttgg tcttcagtta agatgaagaa
     6601 ataataaatc agataaataa taccagctat tacagcaaag ctgactaaca ttctgacgat
     6661 tgaaaaaata aaggcatcta gattcattac taatccaatc gcagcaataa taagtacaag
     6721 ataaaatatt atattacgca ttaaaagact cctatcatga ttttgcattt aagtttaatt
     6781 ttgacaacgc ttgttgtcct tttgtaagtt gttttttatc tttattttga tagccatttt
     6841 taatatcatc aaatgcatga ttcaaatcag agtttaattt atcgatattt ttagacgttt
     6901 ttttatcatc atcactaagt gataaaccat cgatagcatc tttgtatgaa gtgtgagcac
     6961 tttgaatatc attagaaatt tgatttaatt tcgattgaac attcttatcg cctttgtttt
     7021 tacttacatc tttctcaata tcgttatagg atttcaaagc tttagcaact ttgtcgtagt
     7081 atgttgatgt aacattcatc aatttaattt tcgtattatt agatgcagtt tttttactgt
     7141 tttctttatc cttttcaagc cctttaattt tagatttaga atcactaatt tgttgattaa
     7201 gttcttgaat atctagtttg agttgatgat tatcttctct taaatctgtt gttttttctt
     7261 ctaatggtgc taaattttgt gatccacatc cagctaacaa taatgttgcg ccaacaattg
     7321 aaaccaattt tttcattact atctccctaa aatcaatatc tattattttt tatcctcatt
     7381 atgatattat tttaatgtat aaagttgaaa tgcacaaacg tttcactagg aggtaataag
     7441 atgagcagaa taacacaagt gcatcgtatt ttagaacaaa aacatttaga tgcgattatc
     7501 attctttcgg attacaatag aagatattta tcaggtttta ctggtaccag tggagcactt
     7561 atcatatcta aagataaaca atatttgata acagacttta gatatattga tcaagcaact
     7621 aagcaagcgc caaattatga aattattaat cgtaaatcta ctattattgg tgagattaaa
     7681 gaattgctac accaagaaaa ttttgaaaat gtaggttttg aggggcacca tgtaagttat
     7741 gatacatacc ttgaattaaa taaaagccgt atatcattaa taagcatttc taatactgta
     7801 gataaaatta gagacgtcaa agatgctgag gaaattgctt taattcaaaa agcagctaat
     7861 attgttgatg aaacatatga atatatttta actgttgtaa aagcaggtat gactgaaaaa
     7921 gaattaaagg caatattaga aagtaaaatg ctagaattag gagcagatgg accatcattc
     7981 gatacgattg tagcatctgg tcatagaggt gcattaccac atggtgttgc aagtgacaaa
     8041 attattgaaa aaggcgacat gattacatta gattttggcg cgtattataa cggctattgt
     8101 tcagatatta ctagaacatt tgctattgga gaaccagatc ctaaactgaa agaaatatat
     8161 caaatagtac ttgaatctca aatgaaagca attaatgaga ttagacctgg catgactggt
     8221 gcagaagctg atgccatttc aagaaactat ttagagtcaa aagggtatgg aaaagaattt
     8281 ggacattcac taggacatgg tattggttta gaaatccatg aagggccaat gctggctcgt
     8341 acgatacaag ataaacttca agttaacaac tgtgttacag tagaacctgg tgtttatata
     8401 gaaggtttgg gcggtataag aatagaagat gatattttaa ttacagaaaa tggttgtcaa
     8461 gtctttacta aatgcacaaa agaccttata gttttaacat aagcgtgtaa aatgaggagg
     8521 aaactgaatg atttcggtta atgattttaa aacaggttta acaatttctg ttgataacgc
     8581 tatttggaaa gttatagact tccaacatgt aaagcctggt aaaggttcag cattcgttcg
     8641 ttcaaaatta cgtaatttaa gaactggtgc aattcaagag aaaacgttta gagctggtga
     8701 aaaagttgaa ccagcaatga ttgaaaatcg tcgcatgcaa tatttatatg ctgacggaga
     8761 taatcatgta tttatggata atgaaagctt tgaacaaaca gaactttcaa gtgattactt
     8821 aaaagaagaa ttgaattact taaaagaagg tatggaagta caaattcaaa catacgaagg
     8881 tgaaactatc ggtgttgaat tacctaaaac tgttgaatta acagtaactg aaacagaacc
     8941 tggtattaaa ggtgatactg caactggtgc tactaaatcg gcaactgttg aaactggtta
     9001 tacattaaat gtacctttat ttgtaaacga aggtgacgtt ttaattatca acactggtga
     9061 tggaagctac atttcaagag gataatctct aatttgttaa caaatagctt gtattcacta
     9121 tactgattta acgtaagata ttctaaataa gtctcataaa gctattgcct aaaatgatta
     9181 taggttat
//
LOCUS       DBNENW010000104         9042 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNENW010000104 DBNENW010000000
VERSION     DBNENW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.105"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            114..2012
                     /locus_tag="AC1LW8_001944"
     CDS             114..2012
                     /locus_tag="AC1LW8_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HIP4773756.1"
                     /translation="MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTT
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQANTIAKLYKQIGLKGI
                     YFTRDKYK"
     gene            2025..3263
                     /locus_tag="AC1LW8_001945"
     CDS             2025..3263
                     /locus_tag="AC1LW8_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HIP4773757.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGESGQPGAKGDTGKKGEQGTPGKNGTVVSINPYTK
                     MWRIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFEGFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            3269..3664
                     /locus_tag="AC1LW8_001946"
     CDS             3269..3664
                     /locus_tag="AC1LW8_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773758.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKIYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRVSQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            3720..4157
                     /locus_tag="AC1LW8_001947"
     CDS             3720..4157
                     /locus_tag="AC1LW8_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIP4773759.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQITDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSKKEEQQVTWKSSQDSSLT
                     PELSAKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKIN"
     gene            4138..5583
                     /locus_tag="AC1LW8_001948"
     CDS             4138..5583
                     /locus_tag="AC1LW8_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIP4773760.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLF
                     GLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDRIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSI
                     QSPTQASKKETAKPQPKAVELKIIKDVVKGYDLPKRGGNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQLGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKQLQLKDYFIKQIRVYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKIGPFLSCPVAYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            6236..6793
                     /locus_tag="AC1LW8_001949"
     CDS             6236..6793
                     /locus_tag="AC1LW8_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001657239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HIP4773761.1"
                     /translation="MGNTTYLKINSENDVDLQDILNDFINCFCKGYVEIKTKYKLLPI
                     FKINFHKNNLPHLLGLHYTHKKVSAKKIIGRIAEGKITHESIKKHYEYSNIKDRLINY
                     NFLHKCFIDKEIRLCVIVPKNSINPQKIDVAFIDGKNSQVMILGLRKSNNNDFYSPAT
                     MYVLGKNSSYRRMRRTHVISIEWKN"
     gene            complement(6994..8931)
                     /gene="isdB"
                     /locus_tag="AC1LW8_001950"
     CDS             complement(6994..8931)
                     /gene="isdB"
                     /locus_tag="AC1LW8_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HIP4773762.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
BASE COUNT         3056 a         1424 c         1717 g         2845 t
ORIGIN      
        1 gggattttag gatatagctt ctgggcgtgc ttctggtttg gtaaatgtaa ataacagtta
       61 agagtcagtg cttcggcact ggctttttat tttgattgaa atgaggtgca tacatgggat
      121 tacctaatcc aaaaaacaga aaacctacag ctagtgaagt agtagagtgg gctttgtata
      181 tcgctaaaaa taaaatagct attgatgtac ctggttctgg aatgggagca caatgctggg
      241 atttacctaa ttatttactc gataaatatt gggggtttag aacatgggga aatgctgatg
      301 ctatggctca aaaatccaat tatagaggta gagatttcaa gataattaga aatacaaaag
      361 attttgtacc acaaccaggc gactggggtg tttggactgg tggttgggca ggacatgtaa
      421 acattgtagt gggaccatgc acaaaagact attggtatgg cgtagatcaa aactggtata
      481 caaataacgc aacaggaagt ccaccttata aaattaaaca ctcttatcat gatggaccag
      541 gtggaggggt taaatatttt gttagaccac catatcatcc agacaaaact acaccggcac
      601 ctaaaccaga agatgatagt gatgataacg aaaaaaataa taaaaaagtt ccaatttgga
      661 aagatgtaac aactataaag tacactattt ctagccaaga ggttaattat ccagaatata
      721 tttatcactt tatagtagaa ggtaatcgac gactcgaaaa acctaaagga ataatgatta
      781 gaaacgcaca aacgatgagc tcggtagaaa gtttatataa cagtaggaag aaatacaaac
      841 aggatgtaga atatccccac ttttatgttg atagacataa tatttgggca cctagaagag
      901 ctgtatttga agttcctaat gaacctgatt atatagttat agacgtatgt gaagattata
      961 gtgcgagtaa aaatgaattt atttttaatg agattcacgc aatggttgta gctgtagata
     1021 tgatggccaa atatgagata cctctaagta ttgaaaattt aaaagtagac gacagcattt
     1081 ggcgttcaat gttggaacat gttaattgga atatgattga caacggtgtt cctcctaaag
     1141 ataaatacga agcattagaa aaggcattac ttaatatatt taaaaacaga gaaaaattat
     1201 taaattctat aactaagcca acagtaacaa aatctagaat aaaagttatg gtagataata
     1261 aaaacgctga tatagctaat gtaagagact cgtcaccaac agccaacaat ggttcggcat
     1321 ctaaacaacc gcagatcata acagaaacga gtccttatac attcaaacaa gcactggata
     1381 aacaaatggc aagaggtaac ccgaaaaaat ctaatgcttg gggttgggct aacgctacac
     1441 gagcacaaac gagttcagca atgaatgtaa agcgtatatg ggaaagtaac acacaatgct
     1501 accaaatgct taatttaggc aagtatcaag gtgtttcagt tagcgcactt aataagatac
     1561 ttaaaggtaa gggaacattg aataatcaag gtaaagcgtt cgcagaagct tgtaaaaagc
     1621 acaacattaa tgaaatttat ttaatcgcgc atgctttctt agaaagtgga tatggaacaa
     1681 gtaacttcgc taacggaaaa gatggagtat acaactactt cggcattggc gcttacgaca
     1741 acaatcctaa ctacgcaatg acgtttgcaa ggaataaagg ttggacatct ccagcaaaag
     1801 caatcatggg cggtgctagc ttcgtaagaa aggattacat caataaaggt caaaacacat
     1861 tgtaccgaat tagatggaat cctaagaatc cagctaccca ccaatacgct actgctatag
     1921 agtggtgcca acatcaagca aatacaatcg ctaagttata taaacaaatc ggcttaaaag
     1981 gtatctactt cacaagggat aaatataaat aaagaggtgt gtaaatgtac aaaataaaag
     2041 atgttgaaac gagaataaaa aatgatggtg ttgacttagg tgacattggc tgtcgatttt
     2101 acactgaaga tgaaaataca gcatctataa gaataggtat caatgacaaa caaggtcgta
     2161 tcgatctaaa agcacatggc ttaacaccta gattacattt gtttatggaa gatggctcta
     2221 tattcaaaaa tgagcccctt attatcgacg atgttgtaaa aggtttcctt acctacaaga
     2281 tacctaaaaa ggttatcaaa cacgctggtt atgttcgctg taagctgttt ttagagaaag
     2341 aagaagaaaa aatacatgtc gcaaactttt ctttcaatat cgttgatagt ggtattgaat
     2401 ctgctgtagc aaaagaaatc gatgttaaat tggtagatga tgctattacg agaattttaa
     2461 aagataacgc gacagattta ttgagcaaag actttaaaga gaaaatagat aaagatgtca
     2521 tttcttacat cgaaaagaat gaaagtagat ttaaaggtgc gaaaggtgat aaaggcgaat
     2581 cgggacaacc tggtgcgaaa ggtgatacag gtaaaaaggg agaacaaggc acacccggta
     2641 aaaacggtac tgtagtatca atcaatcctt acactaaaat gtggcgaatt gatggtaaag
     2701 atacagatat caaagcagaa cctgagttgt tggataaaat caatatcgca aatgttgaag
     2761 ggttagaaga taaattgcaa gaagttgaga aaatcaaaga tacaactctc aacgactcta
     2821 aaacgtatac ggattcaaaa attgctgaac tagttgatag cgcgcctgaa tctatgaata
     2881 cattaagaga attagcagaa gcaatacaaa acaactctat ttcagaaagt gtattgcaac
     2941 agattggctc aaaagttagt acagaagatt ttgagggatt caagcaatca ttaaatagtt
     3001 tgtatgcaga taaaaatcat agtcatacaa tcaagcagat tgaaggatta gaaaatgctt
     3061 tatcaaaaaa atcagacata aatcacagtc atgatgaacg ttatctttta tcatcaaatg
     3121 cttttacaaa agaggaagca gataaacttt atcaacctat cggttcttcg cagccgtcac
     3181 tgaatatttg gacgggcagt gaaacagaat ataattattt gtatcaaaaa gaccctaata
     3241 cactttactt aattaagggg tgatttttat ggaaggtaat tttaaaaatg taaagaagct
     3301 tatttacgaa ggcgaagaat atacaaaaat atatgctgga aatatccaag tatggaaaaa
     3361 gccttcatct tttgtaataa aacccttacc taaaaataaa tatccggata gcatagaaga
     3421 atcaacagca aaatggacaa taaatggagt tgaacctaat aaaagttatc aggtgacaat
     3481 agaaaatgta cgtagcggta taatgagggt ttcgcaaact aatttagggt caagtgaatt
     3541 aggaatatca ggagtcaata gcggagttgc aagtaaaaat atcaacttta gtaatccttc
     3601 agggacgttg tatgtcacta taagtgatgt ttattcagga tctccgacat tgaccattga
     3661 ataattttaa acgactaatt ttttagtcgt tttttatttt ggataaaagg agcaaacaaa
     3721 tggatattaa ctggaaattg agattcaaaa ataaagcagt actaactggt ttagttggag
     3781 cgttgttgct atttatcaag caaatcacgg atttgttcgg attagattta tccactcaat
     3841 taaatcaagc tagcgctatt ataggcgcta tcctcacgtt acttacaggt attggtgtta
     3901 ttactgaccc aacgtcaaaa ggcgtctcag attcatctat agcacagaca tatcaagcgc
     3961 ctagagatag caaaaaagaa gaacaacaag ttacgtggaa atcatcacaa gacagtagtt
     4021 taacgccgga attaagcgcg aaagcaccaa aagaatatga tacatcacaa cctttcacag
     4081 acgcctctaa cgatgttggc tttgatgtga atgagtatca tcatggaggt ggcgacaatg
     4141 caagcaaaat taactaaaaa agagtttata gagtggttga aaacttctga gggaaaacaa
     4201 ttcaatgtgg acttatggta tggatttcaa tgctttgatt atgccaatgc tggttggaaa
     4261 gttttgtttg gattacttct gaaaggttta ggtgcaaaag atataccatt tgcaaacaat
     4321 ttcgatggac tagctactgt ataccaaaat acaccggact ttttggcaca acccggcgac
     4381 atggttgtat tcggtagcaa ttacggtgca ggatacggac acgtagcatg ggtaattgaa
     4441 gcaactttag attatatcat tgtatatgag cagaattggc taggcggtgg ctggactgac
     4501 agaatcgaac aacccggctg gggttgggaa aaagttacaa gacgacaaca tgcttacgat
     4561 ttccctatgt ggtttatccg tcctaacttc aaaagcgaaa cagctccacg atcaatacaa
     4621 tctcctacgc aagcatctaa aaaggaaaca gctaagccac aacctaaagc ggtagaactt
     4681 aaaattatca aagatgtggt taaaggttat gaccttccta aacgtggtgg taatcctaag
     4741 ggtatagtta ttcataacga cgcaggaagc aaaggggcaa cagcagaagc gtatcgaaac
     4801 ggattagtta acgcaccttt atcaagatta gaagcgggta ttgcgcatag ttatgtatca
     4861 ggtaacacag tgtggcaagc tttagatgaa tcgcaagtag gttggcatac tgctaaccaa
     4921 ttaggcaata aatattatta cggtattgaa gtgtgtcaat caatgggagc ggataatgcg
     4981 acgtttttaa aaaatgaaca ggcgactttc caagaatgcg ctagattgtt gaaaaaatgg
     5041 ggattaccag caaacagaaa tacaatcaga ttacacaacg aattcacttc aacatcatgc
     5101 ccacacagaa gctcagtatt gcacactggt tttgacccag taactcgtgg cctattgccg
     5161 gaagataaac aattacaact taaagactac tttatcaagc aaattagagt gtatatggac
     5221 ggtaagatac cagttgccac tgtctctaat gagtcaagcg cttcaagtaa tacagttaaa
     5281 ccagttgcga gtgcatggaa acgtaataaa tatggtactt actacatgga agaaagtgct
     5341 agattcacaa acggtaatca accaatcact gtaagaaaaa taggaccatt cttatcatgc
     5401 ccggtagctt accaattcca acctggtgga tattgtgatt atacagaagt gatgttacaa
     5461 gatggtcatg tttgggtagg atatacatgg gaggggcaac gttattactt gcctattaga
     5521 acatggaatg gttctgcccc acctaatcag atattaggtg acttatgggg agaaatcagt
     5581 tagaatgaca tagtcatgtc tatttgagca ggtgcgttac atacctgctt tctatttaca
     5641 tttaaagata aaatgtgcta ttattttact agaacttttt aacatttctc tcaagattta
     5701 aatgtagata acaggcaggt acttcggtac ttgcctattt ttttatgtta taatgtaatt
     5761 acattaccag taaccaatct ggcttaaaac tacatttccg gtagtcaatc cggctatgca
     5821 gaggacttac ttgcgtaaag cagtaagaag ctgactgcat atttaaacca cccatactag
     5881 ttactgggtg gttgtttata tataacgcaa gttaaccaaa actaactcta tctaataaaa
     5941 agtatgaaaa atttactcat atctattgcg tataaagtta aaagatatta tagttaacta
     6001 tgaagaaagt caactctcta ttccgttctt tcttcctaac ttgcattctt tcgtagttag
     6061 ttcgtcaagt aactattaat ttagttatat acaatcagga gtgaattgta tagcccggca
     6121 gaggccatat atctgactgt tggtcccgca ggagacttct tccttgccat cactcatata
     6181 cataatccct acttacatta atgtttgtag ggatattttt taaggggtgt actaggtggg
     6241 gaacacaacg tatttaaaaa taaatagtga aaacgatgtt gatttacaag acatcttgaa
     6301 tgattttatt aattgctttt gcaaaggtta tgtggaaatt aaaacgaaat ataaattgct
     6361 tcccatcttt aaaataaatt ttcataaaaa taatttaccc cacttattag gtttgcatta
     6421 cacacataaa aaagtgagcg ctaaaaagat cattggaaga atagctgaag ggaaaattac
     6481 acacgaatct ataaaaaaac attatgaata tagtaacatt aaagataggc ttatcaatta
     6541 taattttttg cataaatgct ttattgataa agaaatcagg ctatgcgtta tagttccaaa
     6601 aaattcaatt aatccacaaa agattgatgt agcttttata gatggcaaga acagccaagt
     6661 tatgatactc gggttaagga agtctaacaa taatgatttt tatagtccgg cgactatgta
     6721 cgttctgggt aaaaacagtt catatcgaag aatgagaaga acacatgtta ttagcataga
     6781 atggaaaaat taataaattc gcctatcggt gaatcagtat agatcgcatc ttaaatggtg
     6841 tgtttatttt actcccccta caaccaacaa aaccacacca cctattaatt taggagtgtg
     6901 gttatttttg ttggaagtgt gtatcaggta tctggatacc tcatccgcca attaaaattt
     6961 gttaatttaa taattaaata taaagacgat ttattagttt ttacgttttc taggtaatac
     7021 gaatgcaacg atgctactta aagctaataa tgccattaat ggtaatgtca tatctttatt
     7081 tgattcttca ccagtttgtg gtaatgattt tgctttattt tcttgtgtat ttttattgtt
     7141 ttggctttga gtgtgtccat catttgtgtt tttaatgttt gctttttgta atggagcact
     7201 atcttttgct tcgctagaac ctgctgaagt ttgaacaaca tcttttgttg tttttgatga
     7261 agcagttgtt ggttttgcaa cattttgagt cgtagatact accttagttg gagttgtact
     7321 acttgattct acttcacctt tagttggttt tgtagcaggc gttttgtctt tacctgactc
     7381 actagatgcg tcattttctt tttcaacact tggtaattgt ttattgtcat ctttttggct
     7441 gtcttgtttt tgtgattctt tttcaacagg tgatggtgtt ggtttgctag gcgtagctgg
     7501 agtagcttcc ttcttagctg agttatcttg ttgttctttt ttgttagatt tatcggtatt
     7561 ggcttttgta aatgcttctt tatcaacgat tctgacatgg tattgtccat cataatcaat
     7621 cgtttttacg tgaactttaa cgatagcatc atatagagtt ttaccttcaa catatgggaa
     7681 aataattgtt ctagtattat ttttagcatc tttgcttata gttctaacac gttgaccttc
     7741 aaccatgaaa tctttccagt aatcgtcatt agtagtttcc atgaccatat attttttgcc
     7801 gttaagcata cctgttttaa tagggtgttt aacaaaagta tccatcatag attcgttatt
     7861 ctcaacactt tcataaacaa catattttgt atcttgtaaa tcagtcattt tttcatttgt
     7921 tggttgtaca ttttggaatt cagtaatagc tgatttcact tgctcatcta aagctttctt
     7981 tgtatcctct aatttcttct tgtactcagc ctttaatttt tcaggaagtt tatcttgaat
     8041 tttatttaat tcataaactt gtctttctag tgttttcgct tttttatatg gcgctaataa
     8101 tttttcagct ttataatctt cttcagtttt gaatttatct gcactgttat aaattggttg
     8161 tgcgaattcc attaatgtgt aatcgtattt ttcttctttg ttattgaagt gtgttgaact
     8221 aacaatttta acagcttttg ttccgtttga tacagagaag cgaatgtaag cataatcttt
     8281 aacagtatcg tatgatacta atttaattgg caactttttg tcaccttcat aaacttcaaa
     8341 ttttctccaa aattgacctg attgtaatcc taattcaatt tctggttttg aatcagtgaa
     8401 aataactcta gcaggtttaa cagaacttgc ataatgataa aactgttgag ttccatcttt
     8461 ctttttcatt tcaaaatcaa ttggacgaga gtttggtgcg ctatgatctt tgtcttttat
     8521 tgcagggttt ttaatcgctt ctctaagttc ctgattcaaa ataggatatg tattgttagt
     8581 ggcttttgct gctggtttaa cttcttttgt ttccttaggg gctttaactt ctttaacttc
     8641 tttagcttct tttgtttcag aagtaggagc ctcaacttct ttattagata ctgagacagc
     8701 attagctact ggtttagttt ctggagcttt ttcagatgtt gttgttggac ttgcaactgc
     8761 ttcagttttt ggttgtgctt ctgtatttgt accacctgtt tcttcagctg ctgcttgtgc
     8821 ttcgccattt gacattaata ataaaagtgt actaattgct acagatgcaa cgcctagtga
     8881 tgactttcta attgaataaa atgatttaaa ttctttttgc tgtttgttca tgttgtaaaa
     8941 acaactccta attgtatatt atcaactgat aatcattatc aattgattac ttgtattgta
     9001 cattgaaatg gtagttaatt gcaacgttat tttataaaaa aa
//
LOCUS       DBNENW010000105         9000 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNENW010000105 DBNENW010000000
VERSION     DBNENW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.106"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..789
                     /gene="pgeF"
                     /locus_tag="AC1LW8_001951"
     CDS             <1..789
                     /gene="pgeF"
                     /locus_tag="AC1LW8_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIP4773763.1"
                     /translation="NDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMAR
                     YIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDALH
                     GIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVNF
                     DLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAALL
                     NYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            813..1481
                     /locus_tag="AC1LW8_001952"
     CDS             813..1481
                     /locus_tag="AC1LW8_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIP4773764.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFLQKKEALPSDAVIHFIGSLQSRKVKGVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1478..2041
                     /locus_tag="AC1LW8_001953"
     CDS             1478..2041
                     /locus_tag="AC1LW8_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIP4773765.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2053..2343
                     /locus_tag="AC1LW8_001954"
     CDS             2053..2343
                     /locus_tag="AC1LW8_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIP4773766.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2453..3232
                     /locus_tag="AC1LW8_001955"
     CDS             2453..3232
                     /locus_tag="AC1LW8_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIP4773767.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERILFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3256..3873
                     /locus_tag="AC1LW8_001956"
     CDS             3256..3873
                     /locus_tag="AC1LW8_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIP4773768.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTSDNNQQSNDSETTKK"
     gene            4094..6847
                     /gene="ileS"
                     /locus_tag="AC1LW8_001957"
     CDS             4094..6847
                     /gene="ileS"
                     /locus_tag="AC1LW8_001957"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIP4773769.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVDGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSITESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7137..7934
                     /locus_tag="AC1LW8_001958"
     CDS             7137..7934
                     /locus_tag="AC1LW8_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4773770.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            8311..8606
                     /locus_tag="AC1LW8_001959"
                     /pseudo
     CDS             8311..8606
                     /locus_tag="AC1LW8_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8787..>9000
                     /locus_tag="AC1LW8_001960"
     CDS             8787..>9000
                     /locus_tag="AC1LW8_001960"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIP4773771.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIIT"
BASE COUNT         3264 a         1289 c         1681 g         2766 t
ORIGIN      
        1 aatgataatt ttaaaaagca accgcatcat ttaatatatg aagagttatt acaacaaggt
       61 attactctag gtattacaac tagaggcgat ggtttaagtg actatcctaa aaatgctttt
      121 aatatggcga gatatattga tgatcgccca tataatatta ctcaacatca attgcaatta
      181 gctgaagaaa ttgcgtttga tagaaaaaat tgggtgtttc ccattcaaac acatgaaaat
      241 aaagtcgctt gtattacaaa ggatgatata ggcacaaata tagacacttt aactgatgcg
      301 cttcatggta ttgatgcgat gtacacatat gatagtaatg tcttattaac gatgtgttat
      361 gcagactgtg taccagtata tttttatagt acaaaacatc attttattgc attggcgcat
      421 gcaggttggc gtggtaccta tactgaaatt gtaaaagaag tgctaaaaca tgtgaacttt
      481 gatttgaaag acttacatgt cgttattgga ccatctacat catcaagtta tgaaattaat
      541 gatgatatta aaaataaatt tgaaacattg ccaattgata gtgccaacta tattgaaact
      601 agaggacgag atcgtcatgg tattgatttg aaaaaagcca atgctgcatt attaaattat
      661 tatggtgttc ctaaagaaaa tatttatacg acagcgtatg ctacatctga acatttagaa
      721 ttatttttct cttatcgatt agaaaaaggt caaacaggac gcatgttagc attcattggt
      781 caacagtaaa caaggaggag atatgcttgc gtgtgaaaga taatttacaa caaatctcaa
      841 cacaaattaa tgacaaaagt gaaaaaaata atttttcaac aaaaccaaac gtgattgcag
      901 ttacaaaata tgttacaata gagcgagcta aagaagcgta tgaggctgga ataagacatt
      961 ttggtgagaa tagattggaa ggctttttac aaaagaaaga agcattacca tcagatgcgg
     1021 tgatccattt tataggatca ttacaatctc gaaaagttaa gggcgttata aacgacgtag
     1081 attatttcca tgctttagat cgattgagct tagccaaaga aattaacaaa cgtgcagaac
     1141 ataaaattaa atgtttcttg caagtgaacg tttcgggaga agcttctaaa catggtattg
     1201 ctttagaaga tgttgatcag tttatagatg atcttaaaaa atatgacaaa atcgaaattg
     1261 taggtttaat gacgatggca ccattgacag atgatgaagc atatattaga tcgttattta
     1321 aacagttacg tttgaaaaaa gaagaaatac aacgactcaa tttagaatat gcgccttgtg
     1381 atgaattatc aatgggaatg agtaatgact atcttattgc agttgaagaa ggtgcgacgt
     1441 ttgttagaat tgggactaaa cttgtaggag aagaggagtg agccacttgg ctttaaaaga
     1501 tttatttagt ggattttttg taatagatga tgaagaggaa gtagaagtac ctgacaaaca
     1561 acaacaggtc aatgaagcgc cagcaaaaga gcagtcacaa caaacaacaa aacaaaacgc
     1621 aatcaaatca gtccctcaaa aatctgcatc aagatataca acaacgtcag aagaaaggaa
     1681 taaccgtatg tctaattatt caaaaaataa ttcacgtaat gttgtaacta tgaacaatgc
     1741 tacaccaaac aatgcatcac aagaaagttc aaaaatgtgt ttattcgaac cacgtgtttt
     1801 ttcagataca caagatattg ctgatgagct taaaaaccgc cgtgcgacac ttgtcaattt
     1861 acaacgtatt gataaagtat cagcgaaaag aattattgat tttttaagcg gtactgttta
     1921 tgcaatcggt ggagatatcc aacgtgtagg tactgatatt ttcttatgta cgcctgataa
     1981 tgtggaagta gctggaagca ttacagacca tattgaaaat atggaacatt cattcgacta
     2041 aggagttaac atatggatat aaatgtgcta gctacaatat ttaaatttat cctttttgtt
     2101 gttgaaattt attatttcgg catgattata tatttcttta catcttgggt accaagtatt
     2161 agagaaacta aggtaggtta ttttttagcg aaaatatatg aacctttctt acaaccattt
     2221 agaaaagtaa ttccacctat tggaattatc gacatatcat caatcgctgc aattatcgtt
     2281 ttagtattat tccaaaaagg gttactccaa atctttaatt ggattttaat tcaattacaa
     2341 taaattaagc gacataacga ggcaagggta catgatactt tagcctcgtt tttgatatgt
     2401 atttttctga atataagggc aatagatggt attttataat ttttttaagg tagtgattaa
     2461 catagatatt tatcaacact ttagacagga agaatacgaa ttaattgatc agctaacgga
     2521 taaatgtgat caagcggaac agcattatgc accagtatta acgcattttt tagatccaag
     2581 agggcaatat atattggaag tgatttgtgg cagttatgaa gatttaaacg tatcttttta
     2641 tggtggacct aatgctgaaa gaaaaagagc aatcatttcg ccgaactatt atgaacctaa
     2701 agaaagcgac tttgaattaa ctttaatgga aatagattat cctgaaaaat tcgtcacttt
     2761 aaaacatcaa catattttag ggacattaat gtctttaggt atcgaacgcg aacaagttgg
     2821 agatataatt gtgaatgaac gaattctatt tgttttgaca agtagattgg aatcatttat
     2881 tatgttagaa ttacaacgta ttaaaggcgc atcagttaaa ctttatacta ttccagtaac
     2941 agatatgata caatctaatg agaattggaa aaatgaaagt gcaacagtta gttctttaag
     3001 gttagatgtt gttattaaag aaatgatacg taaatcacgt acgattgcga aacaactaat
     3061 cgaaaaaaaa cgtgttaaag tgaatcacac tattgttgat tcagcagatt ttcaattaca
     3121 agcaaatgat ttaatatcca tccaaggttt tggtagagca cacattactg acttaggtgg
     3181 taaaactaaa aaagataaaa cgcacattac ctatagaaca ttattcaaat agtaatgatt
     3241 taaggaggat aacaaatgcc ttttacacca aatgaaatta agaataaaga gttttcacgt
     3301 gtaaagaatg gtttagaacc tactgaagtt gctaattttt tggagcaact aagcactgaa
     3361 attgaacgtc ttaaagaaga taaaaaacaa cttgaaaaag taatcgaaga gagagatact
     3421 aatattaagt cttatcaaga cgtgcatcaa tctgtaagtg atgctttgat acaagctcaa
     3481 aaagctggtg aagaaactaa gcaagctgca gaaaaacaag ctgaagcgat tatagctaag
     3541 gcagaagcgc aagctaatca aatggttggt gacgcggtag aaaaagcacg ccgtttagca
     3601 ttccagactg aagatatgaa acgtcaatca aaagtattta gatcgcgttt ccgtatgtta
     3661 gttgaagcgc aattagactt attaaaaaac gaagattggg attacttgtt gaattatgat
     3721 ttagacgctg aacaagtgac gcttgaaaat attcatcatt tgcatgaaaa tgatttaaag
     3781 ccagatgaag ttgcagcaaa tgcacaaaat aatgcatcaa atacatcaga caataatcaa
     3841 caatccaatg attcagaaac aactaagaag taagaattaa ataaagacag acgcgtaata
     3901 tacatttaac ttttcacagc gaattaggta atggtgagag cctagtaaaa gcatgtatgt
     3961 tatatcactg gctttttaat atttaaataa tgtaatgaga gaactctaag ttgagttaat
     4021 aagggtggta ccgcgagcaa tcgtcccttt taatttaact tagagttttt taaattttta
     4081 aggagtgaaa aaaatggatt acaaagaaac gttattaatg cctaaaacag atttcccaat
     4141 gcgaggtggt ttaccaaaca aggaaccgca aattcaagaa aaatgggatg cagaagatca
     4201 ataccataaa gcgttagaaa aaaataaagg taacgaaaca ttcattttac atgatggccc
     4261 accatacgcg aatggtaact tacatatggg acatgccttg aacaaaattt taaaagactt
     4321 tattgtacgt tataaaacta tgcaagggtt ctatgcacca tacgtaccag gttgggatac
     4381 acatggttta ccaattgaac aagcattaac gaaaaaaggt gttgaccgaa agaaaatgtc
     4441 aacagctgaa ttccgtgaga aatgtaaaga atttgcttta gaacaaattg aattacagaa
     4501 aaaagatttt agacgtttag gtgttcgtgg tgactttaat gatccatata ttacattaaa
     4561 acctgaatac gaagctgcac aaattcgtat ttttggagaa atggcagata aaggtttaat
     4621 ttataaaggt aaaaagccag tttattggtc tccttcaagt gagtcttcat tagcagaagc
     4681 agaaattgaa tatcacgata aacgttcagc atcaatttac gttgcatttg acgttaaaga
     4741 tgacaaaggt gtcgtagatg cagatgctaa atttattatc tggacaacaa cgccatggac
     4801 aattccatca aatgttgcga ttaccgttca tcctgaatta aaatatggtc aatacaatgt
     4861 agatggcgaa aaatatatta ttgcagaagc cttatctgac gctgtagcag aagcactgga
     4921 ttgggataaa gcatcaatca aattagaaaa agaatacaca ggtaaagaat tagagtatgt
     4981 tgtagcacaa catccattct tagacagaga atcgttagtg attaatggtg atcatgttac
     5041 tacagatgct ggtacaggtt gtgtacatac agcaccaggt cacggggaag atgactatat
     5101 tgttggtcaa aaatatgaat tgccagtaat tagtccaatc gatgataaag gtgtatttac
     5161 tgaagaagga ggccaatttg aaggaatgtt ctatgataaa gctaataaag ccgttactga
     5221 tttattaaca gaaaaaggtg cactattaaa attagacttt attacacata gctatccaca
     5281 cgactggaga acaaaaaaac ctgtaatctt ccgtgctaca ccacaatggt ttgcctcaat
     5341 cagtaaagta agacaagata ttttagatgc aatcgaaaat acaaacttca aagtaaattg
     5401 gggtaaaaca cgtatttaca atatggttcg tgaccgtggc gaatgggtta tttctcgtca
     5461 acgtgtgtgg ggtgtaccgt taccagtatt ttatgctgaa aatggcgaaa ttatcatgac
     5521 gaaagaaaca gtgaatcatg ttgctgattt atttgcagaa cacggttcaa atatttggtt
     5581 tgaaagagaa gcgaaagact tactaccaga aggatttaca catccaggca gccctaacgg
     5641 tacatttact aaagaaacag acattatgga cgtttggttt gattctggtt catcacaccg
     5701 tggcgtgttg gaaacaagac cggaattaag tttcccagcg gatatgtatt tagaaggtag
     5761 tgaccaatat cgtggttggt tcaactcttc tatcacaact tcagttgcta caagaggagt
     5821 atcaccttat aaattcttac tttctcatgg ttttgttatg gacggtgaag gtaagaaaat
     5881 gagtaaatct ttaggtaatg tgattgtacc tgaccaagtg gttaaacaaa aaggtgctga
     5941 tattgcgaga ctttgggtaa gtagtacgga ctatttagct gatgttagaa tttctgatga
     6001 aattttaaaa caaacatctg atgtttatcg taaaatcaga aatacattaa gatttatgtt
     6061 aggtaacatt aacgatttca atcctgacac agatagcatt actgaatcag agttattaga
     6121 agtggatcgt tacttgctaa atcgtttacg tgaatttact gcaagtacga ttaacaacta
     6181 tgaaaacttt gactacttaa atatttatca agaagttcaa aactttatca atgttgagtt
     6241 aagtaatttc tatttggatt acggtaaaga tattttatat attgaacaac gagattctca
     6301 tatccgtcgt agtatgcaaa cagtgttata tcaaatttta gttgatatga cgaagttgtt
     6361 agcaccaatc ttagtgcata cagctgaaga agtttggtct catacaccac atgttaaaga
     6421 agaaagtgtt cacttagcag acatgcctaa agttgtagaa gtagatcaag ctttattgga
     6481 taaatggcgt acatttatga atttacgtga tgatgtgaac cgtgcattag aaactgctcg
     6541 taatgaaaaa gttattggta aatcattaga agctaaagtt acgattgcta gtaacgataa
     6601 atttaatgca tctgaattct taacttcatt tgatgcatta catcaattat ttatcgtgtc
     6661 acaagttaaa gttgtagata agttagatga tcaggcaaca gcttatgaac atggtgatat
     6721 tgtcatcgaa catgcagatg gtgaaaaatg tgaaagatgt tggaactatt cagaggatct
     6781 tggtgctgtt gatgaattga cgcatttatg cccacgatgc caacaagttg taaaatcact
     6841 tgtataattg aaattgtata aagtactcat acagatgata taaattaaag ctctcttcat
     6901 aatcatgttg tagtttttgt tgacatgatg aagagagttt ttttgtgaat aaaaaaatga
     6961 ccaagttacc ggtcatatat gtaaaaaatg tgcgatttac taaaataaaa attattcagg
     7021 aatggtacaa attctctgag gcatataaat gcgttatagt tgctattctc aattatgttc
     7081 gcgataattt taagtaaaag taagcacaga tattgaattt gataggagtt aattgaatgt
     7141 atcataacag taacgcaaac tttgtcaatg gtatcacttt aaatgtgaga gataagaatg
     7201 aattaaagcc attttatgag gacatattag gattaaatat tataaatgag acattaacat
     7261 cgatacaata tgaagtaggt caaaataatc atgtcattac acttgttgaa ttacaaaatg
     7321 gacgtgaacc tttaatgtcc gaagcgggac tgtttcatat cgcaattaaa ctacctcaaa
     7381 ttagtgattt agctaattta ctaattcatt taagcgaata tgatattcca gttaacggag
     7441 gtatacagcc tgcttcgtta tcattatttt ttgaagaccc ggaaggaaac ggttttaaat
     7501 tttatgttga taaagacgaa gcgcaatgga cgaggcaaaa tgatttagta aaaattgata
     7561 ttagaccatt aaatgtaccg agattagtga gtcatgcaac aaaattgtta tggttaggta
     7621 ttccagatga cgctattata ggtgcattgc atattaagac aattcattta tcagaggtaa
     7681 aagagtacta cctcgattat tttggattag agcaatcggc atatatggat gattattcaa
     7741 tatttttagc atcgaatggc tattatcaac atttggccat gaatgattgg gtatcagcaa
     7801 cgaaacgtgt agaaaatttt gatacgtatg gattagcaat tgttgacttt cattatcctg
     7861 aaacaacaca tttaaattta caaggtccgg atggtatcta ttatcgcttt aatcatatcg
     7921 aagttgaaga ttagtatata ctttgaatgg acgaaccata taatgaatcg tttttaatga
     7981 tctttttata caagctatga aggaggctgg gacataaatc cctaaaaaac agcagtaaga
     8041 taattttcaa ttagaaaata tcttactgct gttctctatt tatacaatac ttcgtattga
     8101 atggcttcgc tttgcccatc tggcacatta ctgtaaaatt ctataaatag aatttttgat
     8161 gatgggtccc ttcctagggt accgtctcag ctaccccaac cggcacattg ttgtaagctg
     8221 actatatgtc agcttctgtg ttggggcccc tgtcttcgac tggcactgct ccctcaggag
     8281 tctcgccatt aatactacgt attaacatgt aattttactt tgaaatactt taaagcccaa
     8341 attaatcaaa agctttctga accagaaacg aaaaaatcta tagttaaaga aaaattgatg
     8401 tagagcctgt ttttggattt atgaaggcta ttttgggttt cactcgaatg tcagttcgag
     8461 gaataaataa agttaaacga gagctaggtt ttgtattaat ggcacttaat ataaggaaaa
     8521 tagcagctca acgagctgta cattataaaa tacatatcaa aaaagctgat ttctatcaaa
     8581 taattaatag aaaactgctt ttttaaattg cctaagaact taatatccca gcctgctttc
     8641 ctaattttta atgtcaaaac atagtagttt atcaagtatt gagtgagtaa cattagattt
     8701 aatgtaatat gcttactttt tttattagca ggtgtaagct ataatataaa gagttgtctc
     8761 atggacgatt gattggagga acgaaaatgc acaaaaaata ttttattggc acttccattt
     8821 taatagcagt atttgtcgtt atatttgacc aagttactaa atatattata gctactacaa
     8881 tgaaaattgg agattcattt gaagtgatac cgcacttttt aaacataaca tcacatcgaa
     8941 ataatggtgc tgcatgggga atattgagtg gaaaaatgac atttttcttt attattacca
//
LOCUS       DBNENW010000106         8961 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNENW010000106 DBNENW010000000
VERSION     DBNENW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.107"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(240..1598)
                     /locus_tag="AC1LW8_001961"
     CDS             complement(240..1598)
                     /locus_tag="AC1LW8_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HIP4773772.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1715..3268)
                     /gene="gntK"
                     /locus_tag="AC1LW8_001962"
     CDS             complement(1715..3268)
                     /gene="gntK"
                     /locus_tag="AC1LW8_001962"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA];
                     GO_process: GO:0019521 - D-gluconate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIP4773773.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQKHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHMPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(3292..3972)
                     /locus_tag="AC1LW8_001963"
     CDS             complement(3292..3972)
                     /locus_tag="AC1LW8_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP4773774.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4126..4890)
                     /locus_tag="AC1LW8_001964"
     CDS             complement(4126..4890)
                     /locus_tag="AC1LW8_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP4773775.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNKASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWQVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5055..5747)
                     /locus_tag="AC1LW8_001965"
     CDS             complement(5055..5747)
                     /locus_tag="AC1LW8_001965"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIP4773776.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5979..6395)
                     /locus_tag="AC1LW8_001966"
     CDS             complement(5979..6395)
                     /locus_tag="AC1LW8_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIP4773777.1"
                     /translation="MPFTMKGYPEKWKDFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(6534..8357)
                     /locus_tag="AC1LW8_001967"
     CDS             complement(6534..8357)
                     /locus_tag="AC1LW8_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIP4773778.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDNFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHIMKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNSFCERDKNV"
BASE COUNT         2800 a         1739 c         1309 g         3113 t
ORIGIN      
        1 ttttcaaatt aaattcatca ttatctaatt taaaacaata tactaagtgt ttcataatta
       61 tcgcctgtac aatacgcaca aaaacatgtc ttgaaacgcc tttcattaca ctaaaatccc
      121 caatatactt tttatatcgt tcggattatg actatttcag acgattttct gcataaaaat
      181 aaacgtgttt caaggcaata tattgcaatt accttaaaac acgtttactt aatatttagt
      241 taaacaaata agctgatgaa tagaatgaag atgatacctg aaacggaaat aatcgtttct
      301 aataatgacc atgttaagaa tgtttctttt acagttaaac caaaatattc tttaaacatc
      361 caaaatcctg cgtcatttac atgagacaaa atcacactac ctgcacctat cgcaagtaca
      421 actaatgcaa catttacatc tgatgattgt aataatggta agacaatacc tgtagttgaa
      481 atcgcagcta ctgtagccga acctaatgcg atacgtagca cagctgcaac aatccatgct
      541 agtaaaattg gagacatctc tgtaccttca aacattttag caattgtatt tccgacacca
      601 ccgtcaatta atacttgttt aaatgtaccg ccaccgccaa taatcaataa catcattccg
      661 attggataaa tcgcattcgt cactgattcc ataatatgat tcatcttacg ttttctcatt
      721 aatcccatcg taacgattgc aaataatact gctattagca tggctgtccc tgctgttcct
      781 atcatataaa tgatagattc aaatagattt gtaggtttgt cgtgcccagt tacaagttgc
      841 gttatcgtag acactaacat taatatgact ggtaatgttg cagttaataa actcatacca
      901 aatcctggca tctcttgatc cgtaaattct ttttgtgcac ctaacgctga aatatcgcct
      961 tctcgtgtat acgcagacgg aatcattttt tgtgcaactt tgttaaatat aggtcctgca
     1021 atgagtgtaa ctggaatggc aataatcata ccgtacagta atacatctcc aacatttgcc
     1081 tttaattctt ttgcgatgac taccggtcct ggatgtggtg gtaaaaagcc atgtgtcaca
     1141 gataaagctg ttaccatagg tagtcctagt tttaacactg aaacatttgc gcgttttgct
     1201 actgtaaata ctaatggaat cagtaagact aaacctactt caaagaacaa tgcaataccg
     1261 acgataaatg ctgcaacaag cattgcccat tgtacatgtt tttgaccaaa tttttgaatc
     1321 aacgtgtctg caattcgagt tgcaccacca ccatcagcaa gtaatttccc aagtatggca
     1381 cctaaaccga atattagtgc aatgtggccg agcgtactgc ccatcccttt ctcaatcgtc
     1441 tccataattt tagtcaatgg tatacctagc attaacgctg taatcatcga tgtgataatt
     1501 aatgaaataa atgtatttaa tttaaaccca ataattaata ctaataaaat aacgatacct
     1561 aaaacaacac tgattaacgg ccatatttcg ttaaacatga cattcccctc tttctctttt
     1621 caatagaatg taacaccgtc gtcgcgcacg tattccacat aatatatagt agtcatggca
     1681 caacaacttt atctaagatg cttaagtgac gtatttattg tgttttattt tcagccatat
     1741 gttggcgttg aaaatctgca atttgttcat aattctctgt taaagaacga cttaaattga
     1801 taaaaatgga tacgagctct tggtaaacag cgacattttc ttcaaacggc atatgattat
     1861 ttgtagcacc gaccatcgat gaaacgattg aaaaatcttc aatgtcacct acagctttaa
     1921 gtccaagcac gcaggcacct aagcatgaac tttcatagct ttcaggaacc actaactctg
     1981 tgtcaaatat atctgacatc atttgacgcc atacttcact tttcgcaaaa ccacctgttg
     2041 cttttatcat cttaggcgtt tcattcatta cttcaatgag tgcaaggtag acggtataca
     2101 aattgtaaag aacaccttct aatgcagcgc gaatcatatg ttctttttta tgagataaag
     2161 ttaaaccgaa gaatgaacct cttgcatttg cgttccaaag cggcgcacgt tctccagcta
     2221 aatagggatg gaatattaaa ccatctgcac ctggtttaac acgctttgca atttgagtta
     2281 agacatcata aggatcaaca ccgagacgtt tcgcagtttc gacttcactc gctagcaact
     2341 cgtcgcgcaa ccatctcaat acgacaccac cattatttac aggacctccg atgacgtagt
     2401 ggtcctctgt taagacataa caaaatattc tacctttgta atcagtacgc ggtttatcta
     2461 tcacagtacg aatcgcccca gatgtaccga ttgtgacagc aacttctcct ttaccaacgc
     2521 tattgacacc caaattagaa aggaccccat cactcgcacc aataacaaac ggtgtatctt
     2581 cattaagccc cattaatgtt gcataacgtt ctttcatgcc tttcattacg tacgttgttg
     2641 gcactaattc cggcaacatt tccttggaaa taccaagcaa ttctaatgcc ccaacatccc
     2701 aatctaatgt ttctaaatta aacatccctg ttgcggaagc cattgaataa tcaatgatat
     2761 atgtatcaaa taaatgatag aaaatgtatg ttttaatatc tgcaaactta gcagtacgtt
     2821 gaaatacatc ttgccattca tgtttcatcc aaaaaatttt cactaaaggc gacataggat
     2881 gaataggcgt acctgttcgc tgataaatcg cattgccatc atgcacttca tttattactg
     2941 ttgcatattt tgcagcgcgg ttatctgccc aagtaatatt atttgttaat ctttgatgtt
     3001 tctgatccat cgcaatcaag ctatgcattt gcgcactaaa tgacacaaat ttaatatcat
     3061 ctttattaac tttggattct ctcataacat atttaatagt cattaatacc gcatcaaata
     3121 attcatctgg gttttcttct gagacatcaa cgtttggtgt gtgtaaatca tagccgattt
     3181 gatgtttcat gatgaatgtt ccattttcat catataagac tgacttggta ctcgtcgttc
     3241 caatgtcgac accaatcata tatttcatga caaatccttc tttctttcat tttaattcaa
     3301 ccaaaatcct tcaatatctt taccaacatc gtcgaaattt aagtgaaacg cttctttcaa
     3361 aatttgactg tcgtattgtt ccactgcatc aataaacact tgatgattat gatgtatgcg
     3421 ttcaaaatct tgcgggttct gttgcatacg ttgtcgcatg gatgttaaaa cgagtgccat
     3481 cattactggt tttaaatgac tccaaaatga gtttaaatat tgatgattag atgctttgat
     3541 taatgtttca tgaaattcaa agtcatgctt cgtaaatgat tctgcatcct caaattttac
     3601 tgccactttc atcatttcaa gttgtttctt catttctttt acgataggta gtcgctcttg
     3661 atttttaact cttgaaaatg caaaagactc tagcatcagt cgcaaatcgt acatttcttt
     3721 cttttcttgt tccccaaacg gcaacacatg tgcacccatt ctttctaatt ggatgagttg
     3781 attttgttgc aataatttaa atgcatctcg aattggcgaa cgactcacat taaattgctt
     3841 tgccatttga ttttcagtga gtaacgtacc ttcagctatg tgaccattca caatacctaa
     3901 gcgtaattct gccgcgatac cttctccagt tgtcatacct tccaaccatt tctctggata
     3961 tccatacatc atcaaagcca ctccttcatt acacgacata cttgtataca agtatgttaa
     4021 tatagttctt aatcgtttgc aagcgctttc tttacgcgta ctaaaatagt gaccatccct
     4081 tttcaatgta aatttaaagg gaatggtcac tatcacacga atgatttaat tgttatgttg
     4141 tatgtgggat atttctaatt gttctgtact catatgcgct ttaggtactt caatgcaata
     4201 atgcgtttca tgacagtttg gacattcgaa tcgacgtgtt gtagcagtat gtttcgcttt
     4261 gataacttgc cataaagatg gtgagaatat atgctggcag ttaggacata aataggcaac
     4321 cttttgttgg taataaaaag taacaccaat gccgtatcca atcataaacg gcaacgcaat
     4381 tagaaacggc catttatttt tcatcaaaat tgcacttata atgctagaat attgaattat
     4441 tcctataata cccgcactaa tccaaatgtt acgacgaata cttttcattt cagctgattt
     4501 actcatgaca tgctctatgt cttttaaatg tgtgattgga gacgtcgacg ctttatttac
     4561 gtaatattga acatttttaa ttttgtttaa taccgcttgt tgctgtttga cttgttgatt
     4621 aatttcttgt tgtttcatag ttagtaaagt attgagtgtc ttcaaagtac cttcaccttt
     4681 tagcaacata tctatatcgc ttaacgcaca acctaaatct ttaagcaata agattaactc
     4741 taatgtttgt cgctgttgtt ctgtatacac acgacgcttt ccttctgtaa atccttgtgg
     4801 tttcaaaata cctttgcgat cataatattg aatcgttcgc gttgtcacat tgcataattt
     4861 cgcgagttct ccagtcgaat agttagacat agattccacc tcctacaatc accatagttg
     4921 atgacccgac gtcacgagca agtacttttt ctacatttta aagaaattta ttatactagg
     4981 cgtcttattt ttgtgatttc gtaccatgtt gatttacaaa atcactcaaa ttaagcaaca
     5041 ctactactaa acgtctactc tgttatttca gaatgaattt gttgtaattt atcttcaact
     5101 tcagtaatct ctgtcgcaca ttctttcagt aaatctcgat acttttccgt ctctgcattg
     5161 tttttataac gtattttatg ttctaaactt gcccacatat ccatacctat cgttctaatt
     5221 tgaatttcaa caggcaatac ctcaacgcgt tctgctaaaa agactggaat ggatacaacg
     5281 atatgtaaac tgcggtaacc attttcttta gggtgctgaa tataatcttt acgttttatc
     5341 aattgtacgt cttcttgttt aagcaacatc tcttcaatca aataaatatc atctaagtaa
     5401 ttacatacta cgcgaattcc ggcaatatcc agtatatgtt ctttggcagt ttctgcgcta
     5461 atctgtaatc ctttacgatt aagcttttct attaaactgc gcatttcttt cacacgtcgc
     5521 tccatatgat gtatagggtt gtgtttataa atgtgattga aattatcgtc taaaatactc
     5581 agtttagtgc ttatttcctt tagtgctgac gaataaatat gatctaactc tacgaaacct
     5641 aataacgtat caaatgcttc atcaccattt tcaaatttac ttaaactatt tttaaaatca
     5701 tgtcgcaaat cctctaaata tagtgatggt tttcgatcta catacatttt tatactaacc
     5761 tccgatatat aatcatcttt attgtaccta acattttatt aagataatac taatacacta
     5821 tcgaactttg ggctgatact gaaacagcac aaagaaactc aaacaattcg aattatgtat
     5881 caaaaccttc aattatagga agtaatttta aaatgattta acgcaaaaaa aacacctgtt
     5941 accgttatat aggtacaagt gtttaattga tagagatgtt atacgtcttt aaatgattcc
     6001 acaacttttg gatgtggacc atccataagc ggttctcttt gtcgaacgcc accactttgg
     6061 ttgccaattg attcgctatc aaagtacgat ttatcttgat ttgattgttc ttgaatatgt
     6121 tcttcattat cagttgttgc atattcacta tccacctctg ttttttccat tgttgctgta
     6181 tggaattgta cgaagttctc ttcttctaat gctttgattt cttctttcga taacgctcga
     6241 taccaatctt tcgctttttt cgccgcaata ggaacaacat ctttttcaag atatccttct
     6301 tttaacattg cattcgcaat ttctatcgct ttttgccgtt taattacttc aaaatctttc
     6361 catttttcgg ggtaaccttt cattgtaaag ggcatttcct taacctccaa tatgttataa
     6421 ttcatgttat ataccaccat aattataaaa tgaaacatat taaacacaat tttttagact
     6481 cccgtcataa taacttcaga caaacgttag tctgacctga taaatatttt attttaaacg
     6541 ttcttgtctc tctcacaaaa actattgtgt tgaatattca tcattgctga ttgaatataa
     6601 ttatactgtg gtaaatctgg taatatcttt atttctaatt ctgtatttaa ttctaaatgc
     6661 ttagcgatgt tttcaaatgt tgctaaatac tcgcccatta attgttcatt tattgtaagt
     6721 ctatcttcat tagccatggc tctatttaac ataaagctaa tttcttctaa tgcgaataag
     6781 ctaggataat aattttgaat caatgtctta tcactaaata attcaccatt agctgcatta
     6841 taaacttgcg tcatgttatt taatttcaca gataggttca tagccgtgtt tttctgatac
     6901 acatcatctt tatattggtt ttcagaaaac aaataatgga agagcgttgc ttctttacga
     6961 acaacttcag ccattacatt aggaagcaat gcggatgcgg tacgttgtcc aaggacaaat
     7021 aaacctatga ttgcaatcac aatacccatt gcaacgtcaa taactcttgg aaacgcaatg
     7081 ttaattgtta aattctgtga tgctaatcca ttcattaaaa taacttgtat tgtaataaaa
     7141 actactgcaa tcgcataatt tgctcccacc aatgcttcag taaacaatgc tgcaatgccc
     7201 attaaaatta ctgcaacagg tgttggtatt gaaaacaaca atatgactga aagtactaac
     7261 acacctaaaa tagtacctaa acctcgtgcc atacctctct cgattgcatg tatagttgat
     7321 gttcctagta atattgtatg tgcagataac ggtatccagt atgctttttc aaaatcaaac
     7381 attaacgcaa taaatatcgc tatcatcata atcgctgtat atctcaatgt atttctaaaa
     7441 acaatagagt ctaatgttaa attttgataa atgcgtttac tatataaagg ttttcgcatg
     7501 tctgcttcat acgcaatacg cgcttcattt gcatgcacca tttcatcaat tttcattata
     7561 tggttgaata aattttgaaa ttcctctgtt actgtcactt cttttcgcca atatcttaca
     7621 ttttcctcac tattatctag ttgtgcaatg atatgatcca tcatttcaat taactcatct
     7681 ggcaatggtc gaatttgttt agcgttcaac tctaacagtt cagaataaat cccttgggca
     7741 gatgtgtgta ataataataa tttttggaaa cgtctactta atttatcgtt acttgaagta
     7801 gaagtgatta ataatttatc agatgcttta aaattatcca ccgctgtttt tgctacttgt
     7861 aaaaatgcag atttatcatt ataagtgtgt aacaacttag atatgagttt aaaatcattt
     7921 tgaattgctt gttcttcagc tttgttttta gaaaatacga tcgtgattaa caccatcata
     7981 gtggcaagta taccgcctac taaaatcgca aagcctctat ataatgcttc ttcgggagct
     8041 ataggtaagt taatgggtag actgaatgtc acaatgaaga atgtcgatga cggtccagct
     8101 atttttaagg cagtaaatat ataaaatggt ataaccgtaa caattaataa tgtcattcca
     8161 aaaacgagtg gcgttttggc tgttagcgta ccaagcatca tacatattgc aaacgctaaa
     8221 ttacaaatta ttacagttcg cagcttagat cgcgacggac ctttaaaaac ataaatatgg
     8281 gctagtgttc cggttgcaac taataatcca aattggaaat taccacataa gtaaccgatt
     8341 attgcgggaa taatcattaa cagtccctgt cttacacctt tataaaagtc taagttaagt
     8401 cgattgattt ttatgagtga tgtcaaaaag atgtcatttc gacttctcct ttccttgtca
     8461 aaatatttcc ataatctttt tattagcata gttaaaatga atttgttgct ataaatttta
     8521 attacgcaac atatgtcatt ataacacctt atactcaatt atctaaattc aaaaactttt
     8581 aaagtccaat taaaattatt ttctaatgtt atttaacatc gttgaacctt tggtcgccca
     8641 cctttgcgct agttaagatt tatttgtgac atgttgaaat tattttagtg aatacgatat
     8701 ttagggttag aaaaacattc acatagactt aaggtttatt tacttcatat tattcctagg
     8761 gttcatgatc caaactatag tgaatttaaa aaagtttata aaacatattt gcctcataag
     8821 cttgaatagt tcctatattc gttatttaaa atgacattcg atgtcagttc atttcttaaa
     8881 atatcgaaag atgattacgt ataatatata tagtgacgta tttacaaata atggcagtaa
     8941 gcagtacaaa atgattacac a
//
LOCUS       DBNENW010000107         8951 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNENW010000107 DBNENW010000000
VERSION     DBNENW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.108"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(394..492)
                     /locus_tag="AC1LW8_001968"
     CDS             complement(394..492)
                     /locus_tag="AC1LW8_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773779.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(697..1293)
                     /gene="recR"
                     /locus_tag="AC1LW8_001969"
     CDS             complement(697..1293)
                     /gene="recR"
                     /locus_tag="AC1LW8_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIP4773780.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1300..1617)
                     /locus_tag="AC1LW8_001970"
     CDS             complement(1300..1617)
                     /locus_tag="AC1LW8_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIP4773781.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1707..3404)
                     /gene="dnaX"
                     /locus_tag="AC1LW8_001971"
     CDS             complement(1707..3404)
                     /gene="dnaX"
                     /locus_tag="AC1LW8_001971"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIP4773782.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSELKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLNQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3473..3997)
                     /locus_tag="AC1LW8_001972"
     CDS             complement(3473..3997)
                     /locus_tag="AC1LW8_001972"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773783.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQALAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4135..4236)
                     /locus_tag="AC1LW8_001973"
     CDS             complement(4135..4236)
                     /locus_tag="AC1LW8_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773784.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSEIDVQFE"
     gene            complement(4309..4578)
                     /gene="ffs"
                     /locus_tag="AC1LW8_001974"
     ncRNA           complement(4309..4578)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC1LW8_001974"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4640..5368)
                     /gene="treR"
                     /locus_tag="AC1LW8_001975"
     CDS             complement(4640..5368)
                     /gene="treR"
                     /locus_tag="AC1LW8_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HIP4773785.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5393..7033)
                     /gene="treC"
                     /locus_tag="AC1LW8_001976"
     CDS             complement(5393..7033)
                     /gene="treC"
                     /locus_tag="AC1LW8_001976"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HIP4773786.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKFDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKKILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLTILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7097..8524)
                     /gene="treP"
                     /locus_tag="AC1LW8_001977"
     CDS             complement(7097..8524)
                     /gene="treP"
                     /locus_tag="AC1LW8_001977"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HIP4773787.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
BASE COUNT         2828 a         1955 c         1210 g         2958 t
ORIGIN      
        1 aactttttaa aattaatttt cgttagtaat tcaagttatt tcttaagttg aattgttttg
       61 tttgcctcaa caaataaata ctatacaggc tttcaaactg cttaacaaca gtaaaaatta
      121 cacctttgtc atctgtactg aacgccctta tcctaaaaat cgaacttaaa aaaatcccca
      181 cttataaata gtacgttttt tagaacaaga aacagatttt tttctaacca tatcaatcaa
      241 aaaaaccgca aaacagaacg ttattttttt accagaaatt aaaaataaat ttacaagttt
      301 taaataatct ctcaaaacac cgctatacag gctgatttaa taagctttag accaaagaag
      361 tacaacttac taagccagca tctatcattt caattacaaa agaatttcat ttgcatagtc
      421 attaaccgca tttatcaaat taatagcacc aacacaccat aaccatagat ctaattcgat
      481 aatccaattc acatcattac tattagaagt aacaaatcac aaccaacggc ttgcttccta
      541 aagattaccc atccctcagt aaaactaggt aaaccatacc accgccacac cgcaaataaa
      601 gtagtacatc acaccacacc ataaaaaaag ccacaatgat tacactgtga cttttcttac
      661 tatagtatta aaatcaaaaa tgtttaatag aagacattac atttctgttc tacctgcgat
      721 tgctttagat aatgttactt cgtcagcata ctctaaatcg ccacctaccg ataacccttg
      781 tgctaatctc gtcactttga tacctatagg cttaactaat ctagaaatat acatggctgt
      841 agattccccc tctaagttcg ggttcatagc taagattaat tcgctaactt catcgttttt
      901 caagcgttca atcaatgaag gaatattaat atcttctggt ccaatgccat ccataggcga
      961 aatagaccca tgtaaaacgt gatataaacc tttgtattct ctcatttttt ccatagctat
     1021 gacatctttg tcatcttcca caacacaaat aactgaacga tctctttgct tatcttcaca
     1081 aatataacat ggatcatttt cagtaatgtg accacatacg ctacaatatg ttaattctct
     1141 cttaacatct actaatgctt tggcaaactg aacaacatcg tcttctttca tatctaaggt
     1201 atgaaaagcc agacgttggg ctgtctttgg accaatgcct ggcaatttca taaagctatc
     1261 aataagtttt gatataggtt ctggataatg catctatgat cacattccag ggatgtttaa
     1321 gccttgagta tgtttaccta aacgttcttg agtaagctca tcagctttat tcatcgcttc
     1381 attagtagct gctaacacta agtcttgtag catttcaata tcatctgggt ctacagcttc
     1441 ttctttgatt tcaacgtcga caacttcttt atgaccagtt acagtaactg caaccatgcc
     1501 accgccagct gttcctacaa tacgctcttc tttaagtttt tcttgttctt gagccatttt
     1561 cttttgcatt ttttgcattt gtttcatcat ttgttgcatg tttccgccac cgcgcatatt
     1621 ccatttcctc cttgaaaatc aataaatatt tatcaataaa atgatgtttc tttcattata
     1681 catacgatta tatcgcttgt catgtatcac tcttcatcta tcacatgtac agtttcttca
     1741 ccgaaaagat cttttgcttt ttgagcaata tctgtttgtt gtgcttgttg ctttggcata
     1801 tcatcgcctt cgtttttacg attttgtaaa tactccgttc gaactctttg ccattgatct
     1861 gatggtacac caacaacttt aacgttttta ttaacgatat tacatacaac actttctata
     1921 ctactacgtt tctcgtcgtc tttattgacg atttcacaat ggatctcttc ctcaaatttc
     1981 acaagtacgt gatcttcact tgccgccaca ggttccgaat tttgcaataa actaacgagt
     2041 gattttttat cattgttttt ggcatgatca atcacttctt gccaatgatc tttcaacaat
     2101 ttgatatctg ccttattcgc tttatctagc acttttgcaa tttgttgcat tgaaaatgca
     2161 tttttagatt tttgtatgcc tctcgcaggc tttttcgaag atttttgaac aggagcgaca
     2221 ctcactcctt gtgcttttag tgtttttagt tcttgctcta actgttccat acgttgcaac
     2281 aatacatctg tgtttggcga tgaagcaatt tgtgctggtt cagctacatt cgcaatcact
     2341 tgtggttgac ccttaatctg ctcagctaat tttactaaca acacttcaaa atgaacgttt
     2401 tgattcacac taaaacgaat cgacactaat gtatcattaa taagatcaat catttgatat
     2461 aacatatcta attctaagtt catcagtgct cgatactcag tatctttctc agatgtttta
     2521 ttcataatcg tatctctgac aaaataaatc atatcattta ttaggcgatt cacttcttta
     2581 ccctctgtta taaactgatg gtatttttta aaagatgctt gtacgtcacc ttgtacaata
     2641 tcatcaaaca agtgatccaa cgcttcatca tgtacgctac ctgtgacatt caacgcatct
     2701 tgcaatgtta acgtaccatc accaaatgca atagcctgat ccataatact taatgcatca
     2761 cgcatacccc cttcagacgc tttagcgata aatgccaagg cttcatcttc acattcaatt
     2821 tgttgtgcat ctgctacaaa ttttaaacgt tcaacaattt gatttaggct aattgcttta
     2881 aaatcaaaac gttgtgccct agaaatgatt gttggaggga ttttatgtgg ttctgtcgtt
     2941 gccaatataa aaatagcgtg tgctggaggt tcttctaacg tctttaaaag ggcattaaaa
     3001 gcacctgttg ttagcatgtg cacctcatct ataatataaa ctttatattt caattcactt
     3061 ggtgcatatt taactttgtc tctaatattt cttatttcat caacgccatt attactagca
     3121 gcatcaattt ctatcacatc tgaattagtc ccctgcgtaa tgcctttaca aatatgacat
     3181 tcattacaag gttctccatc agtgctattt aaacagttga ttgctttagc aaacactttg
     3241 gcaatactcg ttttccccgt gcctctcgga ccactaaaaa tataagcatg cgactgtttt
     3301 tctttagaaa tcgcattgcg caatgtcttc gtgacatgtt cttgtccgac gacatcctcg
     3361 aaactttggg gtctgtacat acgatataag gcttgataat tcaagttagc acctccataa
     3421 acaattacct ctcattatag catgataata cctttacttc ttaattgaac aattaataaa
     3481 aatgttctgg aaattttacg attccgtgtg gtgcatccgt caaattatcc cataaatatt
     3541 ttacaagtaa cggtgcatca ccagattcta atttaatgtc atgctcagca gcattttgat
     3601 aacccaactt ttcaaagtag tcaaaacaat ggtctacaac aaccgtacta tactcttgtg
     3661 ctttggcacg ctcttctact gcttgaacca agccacgacc taatttttgt ccacgtaatt
     3721 caggatgaac tgataaagag gcaatcgcca aaccataata cgtcttatca tcactattaa
     3781 tttctacttc aattaataaa acgtgtccaa cgacatcgtt attttcattt ttcgctatta
     3841 cttctaattc aaaattatag caaggagatt ttcttaaatg ttttactttc gcaagtgctt
     3901 gccaactcgt ttcaggatta tcatcaaaac tttcttcaat actatttaaa gatttatcat
     3961 aatctaactc tgttaaagta cttaaatata tttgcatatg tcctccgtag gcatttgatt
     4021 gtcaataatc ataacgtatc tcattaataa ttctattgta agatactccc cctcatttca
     4081 ccatcatttc tttatcatca aataataact tcttactttt cattgacaca aaaatcattc
     4141 aaactgcaca tcgatttcac ttgaatcaaa cttcacatat aaaaaagcta cttcccacaa
     4201 acatgttcca cgtataatac gctgaattgt cttcaagaaa gtagcttcta taaataaata
     4261 tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc gtgcacctaa
     4321 gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg ctaccctacg
     4381 gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt tcatgggttc
     4441 atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt cgcaaaaata
     4501 cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag ggaaccgcta
     4561 cctccccacc tagcacggca agatatatat tactatattt taatagttaa ttgcaagtat
     4621 aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcgtta aatttaaatt
     4681 catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct aaatacacaa
     4741 tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga tatgcttgtt
     4801 catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga tttaatacct
     4861 tttccaaata atcataaata gaatcactcg caacatcatt acctatgtct gaaacaatcg
     4921 actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga cgagttctaa
     4981 caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact tctggaactt
     5041 catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg cccatttctt
     5101 cttgcatttc tttaaaacta accagttcag aaaatggaaa ctctgtaacc tcctgataaa
     5161 tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc aacaaatcta
     5221 atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca tgttcagacg
     5281 gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac gcctcataaa
     5341 tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta ttttacgcgt
     5401 ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact gtgcctgttt
     5461 gaatcacaac acgaccttct ctagccaatc cttctggcaa atcaacagcc gatgctgaga
     5521 aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca aataaatgat
     5581 catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc gtatgtctta
     5641 attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt gcttgtctaa
     5701 cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct gtaaatccag
     5761 cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac ttctgtccta
     5821 aaatcgttaa cacatccgct tcagcatgcc cttcacttaa caactgatgg taggcattaa
     5881 tcgattcaac atcgcgatat tgtgctattg atgtaaaatg tgggtccgtc ataccaattt
     5941 cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata gctaacattt
     6001 tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac actacccgtg
     6061 gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa atacctcgtt
     6121 gccattgcat cagaattttc tttaacttat ggaaatcaag cttcgcattt gtccactttt
     6181 caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc aattcttggc
     6241 gttctggctg tgtatactta atacaatttt caatcgtcgt cgaagacatt tctcctacag
     6301 tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac tcatgcactc
     6361 taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat tcacctttag
     6421 aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc caatgattga
     6481 ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca gcttgactga
     6541 catcaaataa atgtaaataa tattcatctg tctcagaatc aaacttccat gcattaccac
     6601 cgaatttaga atgccaattt gttggcggcc cgtcttcaga tgatctgaaa aagtaataat
     6661 ctctataagg gttgtcttta gatttacggg cttctttaaa ccattcatgc tccgtcgacg
     6721 tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgagca actttgatta
     6781 acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa ttgctgatat
     6841 catagccatt atcattcata ggtgattcat acactggtgt taaccaaata taatcaacac
     6901 ccaataactt gatataatcc aatttttcta taattccatt gatatctcct ataccattcc
     6961 ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt ctccaatcta
     7021 tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa ataacgaacg
     7081 cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta ctaaaatgag
     7141 acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca atgagatata
     7201 ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca ccaacttttc
     7261 caagtacgtt attcatacca acgattgccc ccaatacaca agatgttgat atcgcagcga
     7321 taaatggata tttcagaggt aagttcacac caaacatggc tggttcagta acacctaaca
     7381 taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta cgacgtttat
     7441 agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc gcaacgattg
     7501 gccataagta cgtaccacct aagctgctac ccatcaattg gaaatctact gctaaaaaca
     7561 tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt gctccgccaa
     7621 gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta ccaatcaata
     7681 acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc gctacgggtc
     7741 caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt tcaattttag
     7801 ctagaacata agctgcaatt aaaactggca acacttgacc ttggtaattc aactgcttaa
     7861 tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca tactgagata
     7921 ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga ctaccaccaa
     7981 atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc gtactcgcaa
     8041 tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata agtgcttttg
     8101 gaccaaataa acctttcatt gtaagtaaat tattgatccc cattaacaaa ccagctgtca
     8161 caatcgcagg taatattggt ataaaaatat cccctaacaa tttgatcaaa cgttgtactg
     8221 gattcccttt ttgcgcagct gcttgtttcg cttcatcttt cgaagcttct tgagcacctg
     8281 tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg acaatttgat
     8341 attgatggtc tgctttgaac tgccccttga ccaacgcgtt attacttaat gcgtctttat
     8401 caactttgct ttcatcttta agcactaaac gtaatcgtgt tacacaatgc gttgcagctt
     8461 caagattttc ttttccccca atagcgctta cgatggcttt tacatcttct ctttttacag
     8521 ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga caagttatgt
     8581 aaaggctttc aacaaatatt ttttattttg tcacaattat ctggggtagc catttcagaa
     8641 aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg cgtgattaac
     8701 aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact cctaaattga
     8761 aaaatgcata tctataaata gcaaacttgc ccacacaagc catccatcat atttcacaaa
     8821 acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca atctcaatgc
     8881 cctatcattt ctatagctaa aaaaacgagc cattcaaaga caaataaaaa aggcaaatac
     8941 tcaaaatttg a
//
LOCUS       DBNENW010000108         8916 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNENW010000108 DBNENW010000000
VERSION     DBNENW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.109"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..139
                     /locus_tag="AC1LW8_001978"
     CDS             <1..139
                     /locus_tag="AC1LW8_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HIP4773788.1"
                     /translation="NNTKNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNED
                     I"
     gene            complement(232..393)
                     /locus_tag="AC1LW8_001979"
     CDS             complement(232..393)
                     /locus_tag="AC1LW8_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773789.1"
                     /translation="MLLILELRFWNSEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(430..1170)
                     /locus_tag="AC1LW8_001980"
     CDS             complement(430..1170)
                     /locus_tag="AC1LW8_001980"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIP4773790.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRSLNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDRDATLVIHDDMVSVVKSLMTE"
     gene            1412..2167
                     /locus_tag="AC1LW8_001981"
     CDS             1412..2167
                     /locus_tag="AC1LW8_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP4773791.1"
                     /translation="MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELE
                     NKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPG
                     TTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMAN
                     AMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDF
                     TSFCQLNELTAVVMDYEDEEKVEMIKTYIEVVD"
     gene            2535..2963
                     /gene="lacA"
                     /locus_tag="AC1LW8_001982"
     CDS             2535..2963
                     /gene="lacA"
                     /locus_tag="AC1LW8_001982"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HIP4773792.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2979..3494
                     /gene="lacB"
                     /locus_tag="AC1LW8_001983"
     CDS             2979..3494
                     /gene="lacB"
                     /locus_tag="AC1LW8_001983"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HIP4773793.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3507..4439
                     /gene="lacC"
                     /locus_tag="AC1LW8_001984"
     CDS             3507..4439
                     /gene="lacC"
                     /locus_tag="AC1LW8_001984"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HIP4773794.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4443..5423
                     /gene="lacD"
                     /locus_tag="AC1LW8_001985"
     CDS             4443..5423
                     /gene="lacD"
                     /locus_tag="AC1LW8_001985"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP4773795.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKNQDAATHLPYIHLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5445..5756
                     /locus_tag="AC1LW8_001986"
     CDS             5445..5756
                     /locus_tag="AC1LW8_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP4773796.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5762..7474
                     /locus_tag="AC1LW8_001987"
     CDS             5762..7474
                     /locus_tag="AC1LW8_001987"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HIP4773797.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7492..8904
                     /gene="lacG"
                     /locus_tag="AC1LW8_001988"
     CDS             7492..8904
                     /gene="lacG"
                     /locus_tag="AC1LW8_001988"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIP4773798.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
BASE COUNT         3143 a         1339 c         1703 g         2731 t
ORIGIN      
        1 taacaatact aaaaacttaa tatatcattt atatgatgat agaggtctat ggctagcttt
       61 taataataat gaagattatg caagatattc tgaaaaatat aatgatttaa tatcggaatt
      121 tgataatgaa gatatctaat tttttaatat tcgtagcttt tatttttcta cttattttat
      181 gtttattttt aatcttacaa atgacaaacc attaaaagta gcatcccaac atcaaacaaa
      241 aaaacaattc atcaaataaa aatcgctaca aaaccaagtc attaaacacg caataattaa
      301 aattttccac tcattataat tctgaattcc aaatgtcgaa ttccgaaaac caaactccga
      361 attccaaaaa cgcaactcca aaattaaaag catctccctg ttactcggga aatgcttttt
      421 acctactgat tactctgtca ttaatgattt tacaacggaa accatgtcgt catgtatgac
      481 caaagtagcg tctctatcat aaggtgttcg atctttattg ataataatta aattgtcgcc
      541 tttaaaattt gatattaatc ctgcggcagg ttgtacaacg agtgatgaac ctagtacaac
      601 aagggtgtca gcatgttcaa ttttatttaa tgaccgtatg atggtaggtt gatctaacat
      661 ttcaccgtat aatacgatgt ccggtcgaat ggcaccacca caattatcac aatgtttcaa
      721 agttctatca atgacatctg acttcgtata agatttatga catgcattac aataaaaacg
      781 atttaacgtg ccatgtaatt catcaacatg ttgacttcca gcgtctgagt gcaaaccatc
      841 gatattttgc gtgatgacac ctaaagattg ttgattacgt tctaattttg caatccaatc
      901 atgaacgata ttaggcttcg tatcgacaaa tagtaaacgc ttatggcaga aattgataaa
      961 accttcagga tcatcttcta aataatcacg gcttaacaag tattctggcg aaagtccatc
     1021 ttttgaaatt tcatcaaata agccacccat tgaacggaaa tctggaacgc cacttgcgac
     1081 agatacacca gcacctgtga aaaatgtaat acgattcgaa ctatctataa tatgttttaa
     1141 tgtctctaaa tcatttctca aaataaacac cccatcaaaa taatgaatga tttataacgt
     1201 cttttgcttt tattatagta tattcatttt acgattatca aaagcgcagt ttaaacagtt
     1261 ctttttaaaa atgaattata catataatcg aacagttaaa ttatattagt ttttacgcaa
     1321 tttttattat agaataagca tgtatattaa aattgatgtc taaacataaa ggataatgac
     1381 ccatcgccta aataaaggag gcaattttac attgaataaa catgaacgtt tggacgaaat
     1441 tgctaaacta gtgaataaaa agggcacgat aagaacgaat gaaatcgtcg aaggtttaaa
     1501 tgtgtctgat atgacagttc gaagagattt aattgagttg gaaaataaag ggattttaac
     1561 gaagattcat ggtggtgcac gcagtaattc aacatttcag tataaagaaa tatcgcataa
     1621 agaaaaacat acccgtcaaa ttgcagaaaa acgatttata gctaaaaaag ctgcatcatt
     1681 aattgaagat ggggatactt tgtttttcgg accgggaaca acagtggaac tattagcgga
     1741 agaagtcaat catcatacgc tcacaattat tacgaattgt ttgccagtgt ataaaatttt
     1801 gttggaaaaa caaacagcac attttcgtgt ctatttaatt ggtggtgaaa tgcgccatat
     1861 tacagaagcg tttgtaggtg aaatggcgaa tgccatgtta gaaaaactaa gatttagcaa
     1921 gatgttcttt agtagtaacg cagtaaataa aggtgctgtg atgacatcta cattagatga
     1981 agcctatacg caacaattgg cactaagtaa ttcaattgaa aaatacttgt taatcgatca
     2041 tacgaaagtt ggcaaagaag attttacatc attttgtcag ttaaacgaat tgactgcagt
     2101 ggtcatggac tatgaagatg aagaaaaagt agaaatgatt aaaacatata ttgaagtagt
     2161 cgactaaatt aaaagtataa ctgcattgtt aaaatatgta tctatatgct cataagtgaa
     2221 tgtgaatttg cattcattta tgggctattt tttatgcaaa tttatgtaaa cgggaacctc
     2281 gacaaagttt tactacaatt tcagacgaat acctaattaa aacaacacca cagtatgaat
     2341 gttcacttat tgtttgaaaa tatgtcataa acaaacagat tataaacata ttatgttgat
     2401 ttattgtttg tttatgttta taatttaaac gtaatcaaat tgaaagcctt ttctcaagat
     2461 tttcataacc tacaaataaa catgtcatat tttaagaaag gtatttcaaa attaaagtaa
     2521 aaaggagtct tattatggcg attattattg gttcagatga agctggcaaa cgattaaaag
     2581 aagtcatcaa atcatactta ttagacaata aatatgatgt tgttgacgta acagaaggac
     2641 aggaagttga ctttgttgat gcaactttgg ctgtagcaaa agatgttcaa agtcaagaag
     2701 gtaacttagg tattgttatc gatgcatttg gtgcaggtag ctttatggtt gctacaaaaa
     2761 tcaaaggcat gattgctgct gaagtatcag atgaacgctc tggatatatg actagaggcc
     2821 ataacaattc aagaatgatc actatggggt ctgaaattgt tggagacaca ttagcgaaaa
     2881 atgtcgttaa aggttttgtt gaaggtaaat acgatggtgg tagacaccaa atccgtgtag
     2941 atatgcttaa taaaatgtgc tagggaggaa aaattactat gaagattgca ttaggatgcg
     3001 accatattgt tacagataca aaaatgcgtg tatctgaatt tttaaaatca aaaggacatg
     3061 aagtcattga cgtaggaaca tacgatttca caagaacaca ttatccaatt tttggtaaaa
     3121 aagttggcga acaagttgtt agcggtaatg cagacttagg tgtttgtatt tgtggaacag
     3181 gtgttggtat taacaatgct gtaaataaag tacctggcgt tcgttcagca ctagtacgtg
     3241 atatgacatc agcgttatat gctaaagaag aattaaatgc gaacgttatt ggctttggtg
     3301 gacgtattat aggtgagtta ttaatgtgcg atattatcga tgcatttatt aacgctgaat
     3361 ataaaccaac tgaagagaat aaaaaattaa tcgctaaaat taaacattta gaaacaagca
     3421 atgcagatca agctgatcca catttctttg atgaattctt agaaaaatgg gacagaggcg
     3481 aataccacga ttaagggacg acacatatga ttttaacttt aacattaaat ccatccgttg
     3541 atatttcata tccattaaca gcattgaaat tagatgatgt taatcgtgtt caagaagtaa
     3601 gtaaaacagc tggtggtaaa ggtttaaatg taacaagagt acttgcgcaa gtaggcgagc
     3661 cagtccttgc gagtggtttt attggcggtg aattaggtca atttattgct aaaaaattag
     3721 atcatgccga catcaagcat gcattttata acattaaggg tgaaacacga aactgcattg
     3781 caattttaca tgaagggcaa caaacagaaa ttttagagca gggaccggag attgataatc
     3841 aagaggctgc aggatttatt aaacattttg aacaattatt agaaaaagtt gaagcagttg
     3901 ctatttcagg tagcttacct aaaggattaa atcaagatta ttatgcacaa attattgaac
     3961 gatgccaaaa caaaggtgtg cctgtgatat tggactgctc gggtgcaact ttgcaaacag
     4021 ttttggaaaa tccatacaaa ccaacagtca ttaaaccaaa catttctgaa ctatatcaat
     4081 tgcttaacca gccacttgat gaaagtttag aaagtttgaa acaggcagtg tcacaaccct
     4141 tattcgaagg gattgagtgg attattgtgt ctttaggtgc acaaggtgca ttcgctaagc
     4201 acaatcatac attctataga gtgaatattc cgacaataag cgtattaaat ccggttggtt
     4261 caggtgattc tacagttgct ggaattactt cagcaattct caatcatgaa aacgaccacg
     4321 atttactaaa aaaagccaat actttaggca tgctgaatgc gcaagaagca caaacaggtt
     4381 atgtcaattt aaataattat gatgatttat tcaatcaaat tgaagtttta gaggtgtaaa
     4441 aaatgtcaaa atcgaatcaa aaaatcgcat caattgaaca actaagtaat aacgaaggta
     4501 tcatttctgc attagcattt gaccaacgtg gtgctttaaa acgcatgatg gcaaaacatc
     4561 aaactgaaga gccaactgta gctcaaattg aacaattaaa agtattagtt gcggaagaat
     4621 taacacaata tgcatcttca attttattag atccagaata cggtttacca gcatctgatg
     4681 cacgtaacaa agattgtgga ttattgctag cttatgaaaa aactggttat gacgtaaatg
     4741 ctaaaggtcg tttacctgac tgtttagtag agtggtctgc aaaacgttta aaagaacaag
     4801 gtgcaaatgc tgttaaattc ttactttact atgatgttga tgatgctgaa gaaattaaca
     4861 ttcaaaagaa agcatacatt gaaagaatcg gttcagaatg tgttgcagaa gacattcctt
     4921 tcttcttaga agtattaaca tacgatgaca acattcctga taacggtagt gttgaattcg
     4981 caaaagttaa gcctagaaag gttaacgaag caatgaaatt attctcagaa ccacgcttta
     5041 atgttgatgt attgaaagtt gaagtaccag taaacatgaa gtatgtagaa ggctttgctg
     5101 aaggtgaagt ggtttataca aaagaggaag cggcacaaca ctttaaaaat caagatgcag
     5161 caactcattt accatacatt cacttaagtg ctggtgtttc tgcggaatta ttccaagaaa
     5221 ctttaaaatt tgctcacgaa gctggtgcta aattcaacgg tgtactttgt ggccgtgcta
     5281 catggtctgg tgctgttcaa gtatacattg aacaaggtga agatgctgct cgtgaatggt
     5341 tacgtacaac tggtttcaaa aatattgatg acttaaataa agtattaaaa gatactgcga
     5401 catcttggaa acaaagaaaa taagtataaa aggggattaa tgctatgaat agagaagaag
     5461 tccaattatt aggttttgaa attgttgcat ttgcagggga tgcacgttct aagtttttag
     5521 aagcattgac agcagctcaa gctggagatt ttgcaaaagc agatgcattg attgaagaag
     5581 gaaacaattg cattgctgaa gcgcatagag cacaaacaag cctgttagct aaagaagcac
     5641 aaggtgatga tattgcatac agtgtaacga tgatgcatgg tcaagaccat ttaatgacaa
     5701 caattttact gaaagattta atgaagcatt tattagaatt ttataaaaga gggtgatgac
     5761 aatgatgcaa aaattgatcg cacaaattga aaaagggaag ccatttttcg agaaattatc
     5821 acgtaacata tatttaagag ccatccgtga tggatttatt tctgcaatgc ctgtcatctt
     5881 attctcaagt atctttctat taattgcata cgtgccaaat atatttggtt ttaaatggga
     5941 taaaggcatg gaagccattt taatgaaacc atataactat acgatggggc ttgtagcatt
     6001 tttagtagca ggaacgacag cgaaatcttt aacagattca tttaaccgta agctagaaag
     6061 tacaaatcaa attaacttta tttctacaat gctagcagcg atgtgtggat tcttgttctt
     6121 agcttctgat ccagccaaag atggtggatt cttaagtgca tttatgggaa ctaaaggttt
     6181 attaacagca tttttatcag catttgtaac tgttattgtt tataacttct gtgtgaagcg
     6241 caatattaca attaaaatgc cgaaagaagt accgccgaat atttcacaag tatttaagga
     6301 cttaattcca ttttcagcgg taatcatcat tctttatgca ttagatttag tcattcgcaa
     6361 cagctttaaa tcaaatgtag cggaaggtat tttaaaatta ttcgaaccat tatttacagc
     6421 agcagatgga tggattggtg tcacaattat ctttggtgcc tttgcattat tctggtttgt
     6481 aggaattcat ggtccatcaa tagtagagcc agcaattgca gccattacat atgcgaatat
     6541 cgaagcgaac ttcaagttgc ttcaagctgg agaacacgca gataaaatta ttacatcagg
     6601 tacacaaatg tttatcgtta cttttggagg tacgggtgca acattggtcg tcccgttcat
     6661 gtttatgtgg atgacgaaat ctaaacgaaa caaagcgatt ggacgtgcat cagttgtacc
     6721 aacattcttt ggtgtaaacg aaccaatttt atttggtgca ccactagtat tgaatcctgt
     6781 gttctttatt ccatttgtat tagcaccaat tgttaacgta tggattttca aacttttcgt
     6841 tgaagtgtta ggaatgaata gttttagtgt gaatttacct tggacaacac caggtccatt
     6901 gggcattatc atgggtacag gttttggttt atggtcattc gttctagcta ttactttgat
     6961 tgttgtagat attattattt actacccatt cctaaaagtt tatgatagtg aaattcttga
     7021 tgaagaagaa ggacgtaaag aaagtaattc agatttaaaa gaaaaagttg cagcaaactt
     7081 tgatacgaaa aaagctgatt caattttagc ggcaagtggt gtatcagacg atgcagctaa
     7141 agcttcaaat atcacagaac aaacaaatgt cttagtactt tgtgccggtg gtggtacaag
     7201 tggattatta gccaatgcac ttaataaagc agctgaagaa tatcatgtgc cagtgaaagc
     7261 ggcagctggc ggatatggcg cacatatgga cattatgaaa gagtaccaat taatcatttt
     7321 agcgccacaa gttgcgtcta actatgaaga tattaagcaa gatacagata gattaggtat
     7381 taaattagcg aagactcaag gtgccgaata tatcaaattg acacgagacg gccaagcagc
     7441 ccttgatttc gttcaacaac aatttgaaaa ttaagaatag gagtttttca tatgactaaa
     7501 acattacctg aagattttat atttggtgga gcaacagcag catatcaagc agaaggtgcg
     7561 acaaatacgg atggtaaagg tcgtgtagca tgggatacgt atttggaaga aaactattgg
     7621 tatactgcag aaccagcgag tgatttttat aacagatatc cagtggattt agaattaagt
     7681 gaaaaattcg gtgttaatgg tatccgtatt tcaattgcat ggtcacgtat cttcccaaat
     7741 ggatatggcg aagttaaccc taaaggtgtt gagtattacc ataagttatt tgcagagtgt
     7801 cataaacgtc atgttgaacc atttgtaaca ttgcatcatt ttgatacacc agaagtgtta
     7861 cacaaagatg gcgattttct aaatcgtaaa acgattgact actttgtaga ttatgctgaa
     7921 tattgtttta aagaattccc agaagtaaaa tattggacta cattcaatga aattggacct
     7981 attggtgatg gccaatattt agtaggtaaa ttccctccag gtattaaata tgattttgaa
     8041 aaagttttcc aatctcatca taatatgatg gttgctcatg caagagcagt gaaattattc
     8101 aaggatggcg gctatcaagg agaaattggt gttgtacacg cattgccaac aaaatatccg
     8161 ttcgatccat caaatcctga agatgttcgt gcagcagaat tagaagatat catccataat
     8221 aaattcatct tagatgcaac atatttaggt aagtattctc gtgaaacgat ggaaggtgtg
     8281 caacatatct tatctgttaa tggcggtaaa ttaaatatta cagatgaaga ttatgccatt
     8341 ttagatgcag ccaaagattt aaacgacttc ttaggtatca attactacat gagtgactgg
     8401 atgagaggtt acgatggaga atctgaaatc acgcataatg cgacaggtga taaaggcggt
     8461 tctaaatacc aacttaaagg tgtcggacaa cgtgaatttg atgtcgacgt acctcgcaca
     8521 gactgggact ggatgatcta tcctcaaggt ttatatgatc aaatcatgcg tgttgttaaa
     8581 gattatccta actatcacaa gatttatatc actgaaaatg gtttaggata taaagatgaa
     8641 tttattgaat ctgaaaaaac agttcatgat gatgcgcgta ttgattatgt aagacaacat
     8701 ttgaacgtta ttgcagatgc tattaaagat ggtgcaaatg ttaaaggtta ctttatttgg
     8761 tcattaatgg atgtgttctc ttggtcaaat ggttatgaaa aacgatatgg tttattctat
     8821 gtcgattttg agactcaaga acgttatcct aagaaaagtg cttattggta caaagagtta
     8881 gccgaaacaa aagaaattaa ataattatag atatta
//
LOCUS       DBNENW010000109         8915 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNENW010000109 DBNENW010000000
VERSION     DBNENW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.110"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            3..1502
                     /locus_tag="AC1LW8_001989"
     CDS             3..1502
                     /locus_tag="AC1LW8_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773799.1"
                     /translation="MPIIVVLLLIGGIAWGVYAFFANTPKNTYLKSEQQTAKMYKDYF
                     NDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKMSYGHDPK
                     KEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTYSKLTGED
                     EETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKGKKEEI
                     KVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDFEKDIKKA
                     IDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLEDDKLKLDY
                     ALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQEKVDGT
                     KRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPINFILKSS
                     TKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1570..2736
                     /locus_tag="AC1LW8_001990"
     CDS             1570..2736
                     /locus_tag="AC1LW8_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773800.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALVITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRVLITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2733..3653
                     /locus_tag="AC1LW8_001991"
     CDS             2733..3653
                     /locus_tag="AC1LW8_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4773801.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQQSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3667..4326
                     /locus_tag="AC1LW8_001992"
     CDS             3667..4326
                     /locus_tag="AC1LW8_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4773802.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENINFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4378..5328)
                     /gene="lytM"
                     /locus_tag="AC1LW8_001993"
     CDS             complement(4378..5328)
                     /gene="lytM"
                     /locus_tag="AC1LW8_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HIP4773803.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPAIAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGLASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5654..6646)
                     /locus_tag="AC1LW8_001994"
     CDS             complement(5654..6646)
                     /locus_tag="AC1LW8_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HIP4773804.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKAGAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6880..8256
                     /locus_tag="AC1LW8_001995"
     CDS             6880..8256
                     /locus_tag="AC1LW8_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4773805.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8384..8776)
                     /locus_tag="AC1LW8_001996"
     CDS             complement(8384..8776)
                     /locus_tag="AC1LW8_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773806.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(8826..>8915)
                     /locus_tag="AC1LW8_001997"
     CDS             complement(8826..>8915)
                     /locus_tag="AC1LW8_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIP4773807.1"
                     /translation="KVIILNNKIFVFEKSIKKHNEMMYIELCD"
BASE COUNT         2969 a         1533 c         1401 g         3012 t
ORIGIN      
        1 taattcctat tattgttgtc ttattattaa taggtggaat cgcatgggga gtttatgcat
       61 tttttgctaa tacaccgaaa aatacatacc taaaaagtga acaacaaaca gcaaaaatgt
      121 ataaagatta ttttaatgac cgttttgaaa atgaagtgaa gttccaagaa aagatgaaag
      181 ataattcatt tttatcttca ttagaattaa gcgcagatgc atcagatgaa attgttaagg
      241 ggcttggtat tcctaaatct gttgttaatg cttcgaaaat taaaatgtca tatggacatg
      301 atcctaaaaa agagaaatca atgattaatc ttgaaccaac aatagcagac tctgcattag
      361 ggaaattcca gttagctgca gataaagata agcattattt cgaatcacca ttatttaaag
      421 ggaaatatag tgttaataat tctgatttat tatcaactta ttcaaaactt acaggtgaag
      481 atgaagaaac agcaaaagaa aatggtatta caaaccaaca actaaattta aatactcttt
      541 ttagtaatgc tcaagcacaa caaagtgact acagcaaaat tgccgaaaaa tattccgaac
      601 ttattgtcga caaattagat gacgataatt ttgataaagg taaaaaagaa gaaattaagg
      661 ttaatggtga aaagtacaaa gttagacctg tcacgttaac acttagcaga gctgacacta
      721 aaaaaattac attagctgta ttagaagaag ctaaaaagga taaagacctt aaaaaattaa
      781 tggaagaaca aggtgctaca aaagactttg aaaaagacat caaaaaagca attgacgatg
      841 tcaaagaaac taaaaaggat gaatttgcta aaattcaatc taaaatttat accgaaaaac
      901 atacgattgt aaaacgagaa attactatta cagacaaaga aaataataaa actaaaatca
      961 aaggtactaa tactttagaa gacgataagt taaaactaga ttacgcactt gatttcgatc
     1021 aagataaata cacgtatgct gaagcgaaat atacaattaa aggcgtatct tctaaggaaa
     1081 aagacaataa atacagtgat aaatacgaat ttggtaaaaa gacagaatat gatgaatcaa
     1141 aaatcaaatt agataaccaa gaaaaagtag atggcacaaa acgtcaagat aaaggtaaaa
     1201 ttactgtcgc attagataaa tatagcgacg aaaatgaatt cacttttgaa aataatatag
     1261 attctgacgt caaaaataac actcagaaat ctacgttaaa tatcggcatc aaatatgctg
     1321 aagaaccaat taatttcatt ttaaaatcta gcacaaaatt gaaagcagat attgattttg
     1381 atgatagtgg tgcgaaagat ttcaatagtc tatcttcaaa agaccgtgaa aaacttgaaa
     1441 aagaaatcga aaaaaatggc ggcaaaatgt ttgaatcaat tttaaaaaag gcatctaaat
     1501 aactaaaata taaaaataag attggaaggt catcttgcct ttcaatctta tcattggagg
     1561 agtggttaaa tgaaaacaat acatttgttt cgcatctacc actctttttt attgaaaaag
     1621 tggtatttga ttatctattt actatttata ttagcggcac tcgttattac attaacgacg
     1681 atccaacatg taacagaaga tgacaatcat tttaatatag gtgtcgtaga taaagatcaa
     1741 tcaagtgaaa cgaaattaat cttaaactct attggtaaag ggagtaacct aggaaaaaac
     1801 gtgagcatta aggcatatga tgataagcaa gcacatactt tgttaaaaaa acataaactt
     1861 caaggctatt ttgtttttga taaaggtatg accaaggcat tttataaaca aggcgaacta
     1921 ccaatttcag tatatacata tgatcaacaa tccatgaaaa gtgtcgtgct atctcagcta
     1981 acagattctg tttaccaacg tcttatgcga tcaatgggtg gcatcttagc ttttcaagac
     2041 ttagcaccga aagcatcaca ttctgacagt atcaatgtta tgactgattt gctgattaca
     2101 ggattaaacc gttcaggtgc atttaactta gaaccgattc atttatacga tacgggcagt
     2161 tattatgcaa ttacaggttt tttaacaacg gtattcatct ttgcactatc tttatttaca
     2221 gttttgaaaa tgaatcaaga tactgtattg aaagcgcgat tgaaaatgtt tcatttttct
     2281 aaagagcgtt tattaatcat tcgtgtgttg attacatggt tttatactat gttatggagt
     2341 atcgttggtg tagtttggat tgtgttcagt attccgaata cctttgaatt atataattgg
     2401 ccaacgttag ccattcattt aagctattat gttacctttt taatactgtg gttattactc
     2461 atcgaacttt taacaacagg cttgttgaat agtattagta aagtcatatt agctattgtc
     2521 atcctggtgt tatctggttt aacaatacct acgatttttt tacaacatat agcaaatggc
     2581 gttttcaata ttcaaccgtt cgcagttgta acaaatcaat tgttagaaat tattttaaac
     2641 aactacattt tagaactgca ccctagtttc tatcttagtt tcatcgcact tttgataatt
     2701 aatttagctg tattggtttg gaggtatcgt caatgaagcc ttatatacaa cttgttgtgt
     2761 tcaagcaatg gttacaatac atcttgctcg taacaaccat tgtcatcgca ctcgtactta
     2821 ttggtatcgg ttaccgtgta gcacatgaca acttcaaaat accgattacc attcaagatt
     2881 tagaccaaac cactgcatca aaatcattcg tcaataaaat taaacaatct gactatgtaa
     2941 ctattaaaaa agtcgatgaa gatgaaagct atattgaaga tgatgttact aaaaaggaag
     3001 ctattttaag tatgcaaatt cctaaaggtt tctctcaaaa attaaaagag aaccgtttaa
     3061 aagaaacgat acagttatat ggtagagatg actttatagg tggtattgct gtagaaattg
     3121 ttagtagttc attatatgaa cagcaaattc ctaacattat ttatgaacac cttgaggata
     3181 tgaaacaaca tcaatccatc gatgctatca acaagtccta tcataaacat acacctgaat
     3241 ctaaaatcaa atttgtgtcg cttactaaac aagcacaaca atctatttca attagcttaa
     3301 tctttgctgt gattttattt gttagcgctg ttcaagtagt acttcattat cgtttaaacc
     3361 aacaagcagc attgcaacga ttatcacaat atcatttaag ccgtttcaaa ctatatagta
     3421 cttatgtaat gacacatacg atcttgttat tgttggtact attggcagtt agtctatatt
     3481 tgtctcaacc actcagctta atattttact taaaatcact gttacttata ttgatttatg
     3541 agataggtat cgtctttatc ttattccata ttcaaacaat aagtcatcga ttattcatga
     3601 catttatata tgcacttgct atgggtatcg tatacttgat tattttcatg taaaggagcg
     3661 taactgatga tagaaattaa taacctttca aagcgttacc gtaacaaaca gattttcaat
     3721 catttaacca tgtcctttga tagtaatcgt ttaaccgtat tacttggtga taatggcgct
     3781 ggaaaatcaa cattacttcg tatgatcgct ggtattgaaa aagctaatga tggaactatc
     3841 aactatttcg gcgaaaaatg gaatcaaaga caaatccaaa atcacatcgg ttatgtgcca
     3901 caagacattg cgttatttga acacatgaca gtggctgaaa acattaattt ttttaaatca
     3961 ctttgtaaaa atccaattaa cgatacaact atcaacgaat atttacagca attaaatttt
     4021 gatgatacgt ctgccaaagt atctacattg tccggtggga ataaacgtaa aattaatata
     4081 ttagtaggtt tactaggtca acctcgaatt ctaattttag atgaaccgac agttggtatt
     4141 gatttgaaat ctagacatga catccaccaa ctgcttaaca ttatgaaatc taaatgttta
     4201 attatattaa ctacccatca tttagatgaa gttgaagcac ttgcagatga tatcaagtta
     4261 attggccaag atccttttta tcaacatgtt ttagaggaca agcaatgggc ttatacctat
     4321 tattaaaacg aaaaaatccc aagttgcgta tgatatcgta acttgggatt tactgtatta
     4381 tctactttgc aagtatgacg ttgggtctac tgcatattga ttaccgatgc caccagacat
     4441 acgttggaag tgtacgtgag gcgctgttga attacccgta ctacctgaat atgcaatttg
     4501 gtcaccagct ttgactttat caccagctga aacagttaaa cgattattat gcatatacca
     4561 ttggtagtta ttactgttcg cttctttaat agttacttga ttgccgccac catagttact
     4621 ccaacctgct tgtactactg tgccatcagt taatgagtaa actggtgaat tttcaggcat
     4681 tgcatagtcg acaccgtaat gcgcaccacc accgtgatat tgtccatatg gttgtagttg
     4741 tttacgactt gttaaccagc ttgcgtcttt cgcatgacca ctagctgtcg ctttacttgc
     4801 taatccgcca ttttgattcg atgtgccatt agggtaattg acctttccat taccatcatg
     4861 gctgttatac gcttggttgt tgttactatg tgaataatag ctcgcgtctg gacctacatt
     4921 tgattgataa ccatattgat taatatgctg ttggctttga ctcgctgtgt agtcattgtt
     4981 atctcctgct attgctggat tcacatatgt ttggccgctt ccattggcat ttgcattttt
     5041 tggataacag ttataaaaat aatgcgtatg tccttgagca tctacgaatg tatagctata
     5101 ttctttatta tcaaacattg cttgattcca gttaccatca ggtgtgtgat gataatcccc
     5161 attagaatca attgtataat aagtaccata agatacgtct tgtgattgtg ttgacatttg
     5221 tgtatgtgct tgttgggtgt ttgtcgtttc tgctgcatct gcttgatgcg ccattgtaaa
     5281 tgtagcgaag cccatcgttg caatcgctgc tgctgttaat tttttcatgt ataaaacatc
     5341 ctccattaaa attaaagtta gttttcaatt aaactgtact gcacatacta aaagaattag
     5401 acaactgagt aaaggattta attctcattt tccaactatt taatattccc gaaatgtttt
     5461 actaaactca ttacattgtc attacaaaat agccatacat tgatattaaa atgacatctc
     5521 tcactgcatt cgtttaacct tttataaatt ttcaaaacta acaactaatc gttcgtccat
     5581 gttcgcgatt caacgctaat gtatgatagt aatcatccat caaatcatat caaccaaatt
     5641 ccattatcaa tcgctattga ttgtcattca actttctaat agtgatatgc ttctcaggct
     5701 taaaaatcgt catatctttt ctattaatta aatcatctgt gagctttaat gctactaatt
     5761 cattgctgcc ataatactta atatataacg ttcttgtagt taaatttatt acggtctgat
     5821 acatcgtata gtgattgtca gcatcatgcg gacgtacaat tccaatcggt atatttaccg
     5881 catctaataa ataaaatgca ttcattaaat ccatttcttt atcattgttt tgagcaatgt
     5941 ttgctttcat aaatgccatt ctcacaaagc gctcagttga agtaaatcca cctggcaatc
     6001 caaatgtacc tgcttcattg cctaaaggtt caatcgttac accttccaat aactttgctg
     6061 ttgctggata aggagaaata ttgatatatt gtcttaaatt actataatgc caatctaagt
     6121 ctggatgatt tgttaagaca ccaatagggt tatcttttat aaccacttca ccctctttaa
     6181 atgaaacttc gactgaatgt ccagttgcat cggaaacatg ataatgcaat ggcggaactt
     6241 caccgatgtc atttaaatat accgctacaa catgtatttg ggatgcttgt tgtttcatat
     6301 cttcaatgct tgttgtatat cccaaaatcc atgtcacaat ttcattttgc gtaatattca
     6361 tcgcgcccgc tttgtgtgtt gatccatatg aactataacc tcggaaatat tgtgttgaaa
     6421 tggcaacgcc atgttcatta acaccatcac cataaataaa accttccata tctgttcctg
     6481 taccaataaa gccatattgc gtttggcctg tcgtgccagt gcgagatgtc caacgataat
     6541 ttctaggcgt cactgctggc gaaccatcta atggataatc ataatccatc gtgcgtccaa
     6601 gaagtacttg attatttaaa gtttgtattg tgaatcctgt gcacattgtt ctcactcctc
     6661 tgtaccttca tttactttaa tcactttcaa ataaagctgt ttcacttaaa catactataa
     6721 aaattcaatc atacaagcaa ttaattgata ttcattctca ataactgtgg tatgatatgt
     6781 aaggaaatca tgactaatgt gtgagtgaac gatcatctat acatccgttc acttcatctc
     6841 atgactttct atatttaatt tttacaagga gtgacatctg tgaataacac acaatcttca
     6901 ccacgcagta atattattat tgcgattatg ttgtctgcat taacatattg gttgtttgca
     6961 caatcattta ttaatatagg acctctcgtt ggtcaaacgt atcaaacctc tcctgccgtg
     7021 ttaaatttat ctattagttt aacttccttc gccacaggta tcttcatggt ggctgcaggt
     7081 gatattgctg ataaaatagg acaactgaga atgacataca tgggtctcat aatcagtatg
     7141 tttgcatctc ttctattaat tatatcggac atcactgcac tgctcatcat cggtagaatt
     7201 ttacaaggtc tatcagcagc tatcttgtta ccttcaacag ttggcgtttt aaataatcaa
     7261 tttaaaggag aacatttaag acgagcgatt agttatctaa tgattagtac tgttgggggc
     7321 atcggcctag ctggtgttat cggcggttta attgccacaa atttcggatg gcaaacgaat
     7381 ttcatcatta gtatagtcat tgctttcatt gccatattac ttctaaaagg cacacctgaa
     7441 aaagtaagtc aacatagcca tcgtcatcca ttcgattaca aaggtatgtc gattttcgct
     7501 gttatgattg gtagctttac attattgtta acacaaggat tcgaacaagg ttggtttagt
     7561 acattttcat tcatttgtct gagcattttt atcatcacta cgttgatatt catcatcatc
     7621 gaacgtcgac atgaagtacc ttttattgat ttctcagtat tacgcaaccg tccgttcatt
     7681 ggtgcatttt taaataactt tgttttaaat agtggcctag gcgtaacagt ggtctttttc
     7741 atatatgctc aaacacacct tggtttatca gctgcgcaat ctggacttgt tacattgcca
     7801 tatgccattg tggcagttgc gatgattcgt ttaggtgaaa aagcaacatt acgtttcggt
     7861 ggcaaattga tgctcatcat tggtccgttg ttccctgtca tcggcatcac tattattagc
     7921 atgactcaat tatcggcatc acaatatgtc attgcagtta tcattggttt cgtcatttgt
     7981 gcaattggaa atggtttagt cgcaacaccc ggccttacaa ttgcaatttt cagtatgcca
     8041 aatgaaaaag ttggtttagc tacaggatta tataaaatga gtggtacatt aggtggcgcc
     8101 tttggtatag cactaagtac tacagttttc agtatgttac aactaaacta tgcaccaagt
     8161 gtagctgcaa ccgtaacatt tatcgtcagc attgtattga tgatccttgg ctcattgtct
     8221 gcatacatga tcattccaaa aacggttaaa tcttaaatat aatagaagca tcatgtttcg
     8281 aaatatctgt atcactttaa aatgatatac aagaaattca agaaaaataa gcgaagaacg
     8341 tttttctctg ttcttcgctt actctaatta tattcactat caattagaca aaatggccat
     8401 tttcaaatat cacgcgttgt ttctgacctt gaatatattt attataattc tctttttgaa
     8461 aatcagttaa cattaattta gatgtaccgt attttaacac tttttgcatt gtttctattc
     8521 tcatttttct aaataaccat ccatctttta acacaatacg attaacagca tcatatgata
     8581 attctactgt ttctttaatt tcaaatgtct tgaatgaaat aatcgtgcac attaaaaacg
     8641 tatcaccaaa gtaataaaca tctaaatcat cacgtttatg ttgtccaact aacaaacgac
     8701 catattcgaa ctctttttct ggatatttca attctaaaaa actaataatc tcttcttctt
     8761 ttaatttgaa ttgcatttaa aaacatcctc tcttatgttt taacaagcct taattaaaaa
     8821 ttttttcaat cacatagttc aatatacatc atttcgttat gttttttaat acttttttca
     8881 aaaacaaata ttttattatt taaaataatc acttt
//
LOCUS       DBNENW010000110         8668 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNENW010000110 DBNENW010000000
VERSION     DBNENW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.111"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(350..423)
                     /locus_tag="AC1LW8_001998"
     tRNA            complement(350..423)
                     /locus_tag="AC1LW8_001998"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(387..389),aa:Arg,seq:tct)
     gene            787..921
                     /locus_tag="AC1LW8_001999"
     CDS             787..921
                     /locus_tag="AC1LW8_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HIP4773808.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            978..1112
                     /locus_tag="AC1LW8_002000"
     CDS             978..1112
                     /locus_tag="AC1LW8_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HIP4773809.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1236..1931
                     /locus_tag="AC1LW8_002001"
     CDS             1236..1931
                     /locus_tag="AC1LW8_002001"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIP4773810.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2040..2480)
                     /locus_tag="AC1LW8_002002"
     CDS             complement(2040..2480)
                     /locus_tag="AC1LW8_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIP4773811.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2675..4288
                     /gene="bshC"
                     /locus_tag="AC1LW8_002003"
     CDS             2675..4288
                     /gene="bshC"
                     /locus_tag="AC1LW8_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIP4773812.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            4432..4863
                     /gene="mraZ"
                     /locus_tag="AC1LW8_002004"
     CDS             4432..4863
                     /gene="mraZ"
                     /locus_tag="AC1LW8_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIP4773813.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            4879..5814
                     /gene="rsmH"
                     /locus_tag="AC1LW8_002005"
     CDS             4879..5814
                     /gene="rsmH"
                     /locus_tag="AC1LW8_002005"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIP4773814.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            5828..6229
                     /gene="ftsL"
                     /locus_tag="AC1LW8_002006"
     CDS             5828..6229
                     /gene="ftsL"
                     /locus_tag="AC1LW8_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIP4773815.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            6210..8444
                     /locus_tag="AC1LW8_002007"
     CDS             6210..8444
                     /locus_tag="AC1LW8_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIP4773816.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNMSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
BASE COUNT         3223 a         1204 c         1634 g         2607 t
ORIGIN      
        1 agtatcaaaa tctataggaa taatataata ataaaaatct ctacgcatgc aaatggtgag
       61 tgttaaaaat cttggtgtag tgttggtgta gttcaggtgc agtatagatg aaatttgagc
      121 acaaaattac ttaattaaaa atgtataaac aagtgtcaga cgcgtgtcaa ttgtgtgtca
      181 aaaaattcta taacgaataa gcatgcttgt tttaattact gtattttcgg ggcttatgcg
      241 ggggacgttt tggtgacgca tcatactata ttactgaaat tcaaaacaaa aagagccccg
      301 taatcacgga actcttttgt ttggtaatgc gtataaaaat acctataaac gtcctgggag
      361 ggattcgaac ccccgaccga tggcttagaa ggccattgct ctatccagct gagctaccag
      421 gacacgttta acaacacaag aattattata tctaaatgaa cttaaattag caataccttg
      481 taaataaaaa atgtttatat ttttcactat tatagagcta tttatctaaa aaggttcaat
      541 aagacttaaa tacgaattca ggcaacttaa ttgtgttaaa tacagttttg aatgcctaac
      601 tgtatttctt ttctctttaa aatacagtta agtacattat aagatgttgt gcggataaac
      661 aaactaattg catcaaattt attttaaaat aacaacaaca aaacgttaag cgaataacat
      721 ttcggtgatt taaaagctac gcacgttttt gttatcttca aatttaaatt ttaaggagtg
      781 ttttcaatgg aaggtttatt taacgcaatt aaagataccg taactgcagc aattaataat
      841 gatggcgcaa aattaggcac aagcattgtg agcatcgttg aaaatggcgt aggtttatta
      901 ggtaaattat tcggattcta atttcaatat gttatgtaag taatcagtat tatttcaaag
      961 gtgagggaga gatttaaatg actggactag cagaagcaat cgcaaatact gtgcaagctg
     1021 cacaacaaca tgatagtgtg aaattaggca caagtatcgt agacatcgtt gctaacggtg
     1081 tgggtttact aggtaaatta tttggattct aatataataa ctaatattct ttaaaataaa
     1141 ctgggtgagc atactttaat gttatgcact cagtttattt tatttgcaga aatttgagcc
     1201 tctgttaaga tttagataca tagacaatat aggagatggg gaaattggga tataaaaata
     1261 ttttgataga ctttgatgat acaattgttg atttttatga tgcagaagaa tgggcgtttc
     1321 actatatggc gaatgttttt aatcataaag caacaaagga tgatttttta acatttaaaa
     1381 aaatcaatca ccaacattgg gaagcttttc aacaaaataa attaacgaag tctgaagtat
     1441 tatcagaacg atttgtgaat tacttcaaac atcatcaaat ggaagttgat gggcatcgtg
     1501 cagatgtgtt atttagaaat ggattagcag aagctaaagt taaatacttt gatcaaacat
     1561 tagaaacaat tgtcgaatta tcgaaaagac atgatttata tattgttact aatggtgtaa
     1621 ccgaaacgca aaagagaagg ttaaatcaga cgccgttgca taaatatatt aaaaagatat
     1681 ttatatctga agaaacagga tatcaaaaac ctaatccgga attttttaat tatgttttta
     1741 atgatattgg tgaggatgaa agacagcact cgattatagt tggagattct ttaacatctg
     1801 acattctagg tggaatcaat gcgggtatag ctacttgctg gtttaatttt agaggatttg
     1861 atcataatcc aggaattata cctgattatg aaattaattc atggaaacaa ctaaatgata
     1921 ttgtacgtta agacaaataa tttgaaaatg atttacttat attagcttaa gaaagtaatt
     1981 gtaaaaaaga cactacgaaa agatgcattt tcatgctcgt agtgtcgaat attattatct
     2041 tatggtttcc acaataagac atcatgtccc tctatatttt tagcttccac atctgtaaat
     2101 ccatgatgta aaaagaaatc cttagaatca tttctgccaa tggctttaat tggcatattg
     2161 aaactctttg caaaatcaat caattctgaa gcgtaccctc tgttttgata ttttggtaat
     2221 acttctaact tccataataa tatataatct tcaaaatctg ggaagtagat ttcttcgaca
     2281 tcaccttttt ttagtaatgc cattctagct cctaattgat ctccgacaaa tatgccataa
     2341 aatggtgaat ctgaacttgc atcaatcatt tgaccgttta actcattgac catgtataag
     2401 tctttgttgc caaacgctct aaagttttcg aataattcgt cagttttgta attaatttca
     2461 agacgtttga tttcactcat atttatctac cccttatttg tttactacta ttattataca
     2521 ttaaaccact tgtgtttttc attgatttga aaatgtaaaa caatgaatat tataaatttt
     2581 ccatgattaa attgattttc aagccgtaaa attgtcaatc accggtatag actttatact
     2641 atagtttgta atcgcgtact taaggagaaa aaacatggac tgtaaagtag ttagtttaaa
     2701 tgaaaaagat cagtttatac caaaaataaa gagcagtgac cctgtaataa caggattatt
     2761 tcaatatgat gcagctcaac aaactagttt tgaaaaaagg atgtctaaag aaaataatgg
     2821 aagagaagcg gcattagcga atgttattcg tgaatatatg agtgatttaa agctttcaag
     2881 tgaacaagaa ttaaacatac aacatttagc taatggttca aaagttgtga ttggtggaca
     2941 acaagcaggg cttttcgggg gaccattgta tacattccat aaaatatttt caatcattac
     3001 tttatctaag gaattaacgg atacacataa gcaacaagta gtaccagttt tttggattgc
     3061 aggagaagat catgatttcg atgaagtgaa tcatacattt gtttataacg aaaatcatgg
     3121 gtcgctgcat aaggttaaat atcatacaat ggagatgcca gagacgactg tctctagata
     3181 ttatcctgat aaggctgagt tgaaacaaac tttaaaaacg atgttcattc atatgaaaga
     3241 aactgttcat acacaaggtc tactggagat ttgtgacaga attattgacc aatatgactc
     3301 gtggactgat atgtttaaag cactactgca tgaaacattt aaagcatatg gcgttctatt
     3361 tatagatgcg cagtttgagc cgttaagaaa aatggaagcg cctatgttta aaaagatttt
     3421 gaaaaaacat cagttgcttg atgatgcttt tagagcaaca caacaacgta ctcaaaatca
     3481 aggcttgaat gcgatgatac aaacagatac aaatgttcat ttattcttac atgatgaaaa
     3541 tatgcgtcaa ttagtttcgt atgatggtaa gcattttaaa ttaaataaaa cagataagac
     3601 atatataaag gaagaaatta taaatattgc ggaaaatcaa cctgaattat tttctaataa
     3661 tgtagtgaca agaccattaa tggaagaatg gttatttaac acggtggcat ttgttggagg
     3721 accgagtgaa attaagtact gggctgaact aaaagatgta tttgaactat ttgatgttga
     3781 aatgcctatc gtgatgccaa ggcttagaat tacttattta aatgaccgta tagaaaaatt
     3841 actttcgaaa tacaatattc cattagaaaa agtgttagtc gatggtgttg aaggagaaag
     3901 aagtaagttt attagagaac aagcatcaca tcaatttatt gaaaaggtag aaggtatgat
     3961 tgaacaacag cgtcgtctaa acaaagactt attagatgaa gtggcgggga atcaaaataa
     4021 tattaacctt gtgaataaaa ataatgaaat tcatatacaa cagtatgatt atttgttaaa
     4081 acgttatctt ttaaacattg aaagagaaaa cgacatcagt atgaagcaat ttagagaaat
     4141 tcaagaaaca ctccatccaa tgggaggatt acaagaaaga atatggaatc cacttcaaat
     4201 tttgaatgat tttgggacag atgtgttcaa gccctccacc tatccaccac tttcttacac
     4261 ttttgatcat attattataa aaccttaata taccaagggt ttagcccgat ttatcttaat
     4321 gataaatcgg gcattttttt gttttttaaa ataaatttca caaattttgt ataaatagtg
     4381 gtggatagtg gggagatgtg gtaaattata tataaggtga ggtgataaaa aatgttcatg
     4441 ggagaatacg atcatcaatt agatacaaaa ggacgtatga ttataccgtc caagtttcgt
     4501 tatgacttaa atgagcgttt tattatcaca agaggccttg ataaatgttt attcggttac
     4561 actctagacg aatggcaaca gattgaagag aaaatgaaaa ccttacctat gacaaaaaaa
     4621 gacgcacgta agtttatgcg tatgttcttc tctggtgctg ttgaagtaga acttgataag
     4681 caagggcgta ttaacatccc tcaaaacttg aggaaatacg ctaatttaac taaagaatgt
     4741 acagtaatcg gtgtttcaaa tcgtattgag atttgggata gagaaacttg gaatgatttc
     4801 tatgaagaat ctgaagaaag tttcgaagat attgctgaag atttaataga ttttgatttt
     4861 taaaatggag gaattgaagt gtttcatcat atcagcgtta tgttaaacga aaccattgat
     4921 tatttaaatg taaaagaaaa tggtgtgtac attgactgta cgctaggtgg agcgggacat
     4981 gccctttatt tactaaatca attaaatgac gacggaagat taatagcaat cgatcaagac
     5041 caaactgcaa ttgataatgc taaagaggta ttaaaggatc atttgcataa ggtgactttt
     5101 gttcatagca acttccgtga attaactcaa atattaaaag acttaaacat tgaaaaagta
     5161 gatggaattt attacgactt gggtgtttca agcccacaac tcgacattcc agaacgagga
     5221 ttcagttatc accatgacgc aacattagac atgcgtatgg accaaacaca agaactaaca
     5281 gcatatgaaa ttgttaacaa ttggtcatat gaagcgttag tgaagatttt ttatcgctat
     5341 ggcgaggaga aattttcaaa acagatagct cgaagaatcg aagcacatcg cgaacaacaa
     5401 ccaataacaa caacattaga attagttgac attataaaag aaggtattcc tgcaaaagca
     5461 agaagaaaag gcggacatcc tgcaaaacga gtatttcaag cactacgaat tgcagtaaac
     5521 gatgaattgt cagcttttga agattcaata gaacaagcga ttgaattagt gaaagtagat
     5581 ggcaggattt cggtaatcac tttccattct ttagaagatc gtttatgtaa acaggtgttc
     5641 caagaatatg aaaaaggtcc agaggtacca agaggattac cagttatacc agaagcatat
     5701 acacctaagt taaagcgtgt taatcgtaaa ccgattaccg ctacagaaga agatttagat
     5761 gacaataaca gagcgcgaag cgcgaaatta cgtgtagctg aaatacttaa ataaggagca
     5821 atttataatg gctgtagaaa aagtgtacca accatatgac gaacaagttt ataatagtat
     5881 accgaagcaa caaccacaaa ctaagcccga aaagaagact gtttcgagaa aagtggttgt
     5941 acaattaact aaatttgaaa aagttttata cataactttg attactgtaa ttgctatgtt
     6001 aagtatttat atgctatctt taaaaatgga tgcgtatgat acgcgaggaa agattgcaga
     6061 tttagattat aaaatagata aacaatcaag tgaaaacagt gctttacaat ctgaaatcaa
     6121 aaagaattct tcttatgaac gcatatacga aaaggctaag aaacagggga tgagccttga
     6181 gaacgataat gtaaaggtag tgcgtagtaa tggcgaagca aaaaattaaa attaaaaaaa
     6241 ataaaatagg ggcagtccta cttgttggtt tattcggact gctctttttt atattggttt
     6301 taagaatttc atatatcatg attactggac attctaatgg tcaagattta gtcatgaagg
     6361 caaatgaaaa gtatttagtt aagaatgcac aacaaccaga acgaggaaag atatatgatc
     6421 gtaatggtaa agtgctagca gaagatgtag aaagatataa acttgttgca gtaatagata
     6481 aaaaggcgag tgccaattct aaaaaaccta ggcatgtagt tgataaaaaa gagactgcaa
     6541 agaaattatc tacagtcatt aatatgaagc cagaggaaat tgaaaagaga cttagtcaaa
     6601 agaaagcttt ccaaattgaa tttggacgca aaggaacaaa tttaacgtat caggacaaat
     6661 tgaaaataga gaaaatgaat ttgcctggta tttctttatt gcctgaaaca gaacgctttt
     6721 atccaaatgg caattttgca tcacacttaa ttggtagagc tcagaaaaat ccggatactg
     6781 gtgaacttaa aggtgcactt ggagttgaaa agatttttga tagttattta agtggatcta
     6841 aaggatcatt gagatatatt catgatattt ggggatatat cgcaccaaat actaaaaaag
     6901 agaagcagcc taaacgtggt gatgatgtcc atttaacaat cgattcaaat attcaagtat
     6961 ttgttgaaga agctttagat ggcatggttg aaagatacca gccgaaagat ttatttgcgg
     7021 ttgtcatgga tgccaaaact ggagaaattt tagcatacag tcagcgacca acatttaatc
     7081 ctgaaactgg taaagacttt ggtaaaaagt gggcaaatga cctttatcaa aacacatacg
     7141 agcctggatc aacatttaaa tcatatgggt tagcagctgc tattcaagaa ggtgcttttg
     7201 atcctgataa gaaatataaa tctggacata gagatattat gggttcacgt atttcagact
     7261 ggaatagagt cggttggggt gaaatcccaa tgtcactcgg atttacttat tcatctaata
     7321 cattgatgat gcatttacaa gatttagttg gtgcagacaa aatgaaatct tggtatgaac
     7381 gatttggatt tggaaaatca actaaaggta tgtttgatgg agaagcacct ggtcaaattg
     7441 gatggagtaa tgagttgcaa caaaaaacgt catcatttgg tcaatcgaca acagtaacac
     7501 ctgttcaaat gttacaagcg caatcagcgt tctttaatga tggtaatatg ttaaaaccat
     7561 ggtttgtgaa tagcgttgaa aatcctgtta gtaaaagaca attttataaa gggcaaaaac
     7621 aaatcgcagg caaaccaata acaaaagata ctgctgaaaa agttgaaaag caattggatt
     7681 tagttgtgaa tagtaagaag agtcacgctg caaactatcg tattgatggt tatgaggtcg
     7741 aaggtaagac tggtacagca caagtcgctg cacctaatgg tggtggatac gttaaaggtc
     7801 caaacccata ttttgtaagt tttatgggtg acgcgccgaa gaaaaatcct aaagttattg
     7861 tatacgctgg tatgagcttg gcacaaaaaa atgaccaaga agcttatgaa ttaggtgtta
     7921 gtaaagcgtt taaaccaata atggaaaata ctttgaaata tttaaatgta ggtaaatcaa
     7981 aagatgacac atctaatgca gagtatagta aagtgccaga tgttgaaggt caagacaaac
     8041 aaaaagctat tgataatatg agtgcaaaat cattagaacc agttactatc ggttctggca
     8101 cacaaataaa agcacaatct ataaaagcag ggaataaagt cttacctcat agtaaagtac
     8161 tgttattaac agacggagac ttaactatgc ctgacatgtc aggatggacg aaagaagatg
     8221 tcattgcttt tgaaaaccta acaaatatta aagtaaattt aaaaggtagc ggttttgtgt
     8281 cccaccaatc aattagtaag ggacaaaaac ttactgaaaa agataaaata gacgtagaat
     8341 tttcatcaga gaatgtagac agcaattcga cgaataattc tgattcaaat tcagatgata
     8401 agaagaaatc tgacagtaaa actgacaagg ataagtcgga ctaacaagaa tggctacatt
     8461 aaataagggt gtattgtcac tatttgtttt tactaaagta atgctgtgat agaaaacaac
     8521 atcttcattt aatctttata aataagtgtt gatagagaat tttaaggtag acgaggtcag
     8581 attaacttgt ttgttatagg caaacttttt atactggcct cttatgccgt aaaaaagaaa
     8641 aattataaat tttgattaca actttgtt
//
LOCUS       DBNENW010000111         8236 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNENW010000111 DBNENW010000000
VERSION     DBNENW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.112"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(64..1257)
                     /gene="metK"
                     /locus_tag="AC1LW8_002008"
     CDS             complement(64..1257)
                     /gene="metK"
                     /locus_tag="AC1LW8_002008"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIP4773817.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(1363..1474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1629..3221
                     /gene="pckA"
                     /locus_tag="AC1LW8_002009"
     CDS             1629..3221
                     /gene="pckA"
                     /locus_tag="AC1LW8_002009"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIP4773818.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(3599..4345)
                     /locus_tag="AC1LW8_002010"
     CDS             complement(3599..4345)
                     /locus_tag="AC1LW8_002010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIP4773819.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(4350..4823)
                     /gene="ytkD"
                     /locus_tag="AC1LW8_002011"
     CDS             complement(4350..4823)
                     /gene="ytkD"
                     /locus_tag="AC1LW8_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA deprotection pyrophosphohydrolase"
                     /protein_id="HIP4773820.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            4889..5146
                     /gene="yidD"
                     /locus_tag="AC1LW8_002012"
     CDS             4889..5146
                     /gene="yidD"
                     /locus_tag="AC1LW8_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIP4773821.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(5143..6144)
                     /gene="menC"
                     /locus_tag="AC1LW8_002013"
     CDS             complement(5143..6144)
                     /gene="menC"
                     /locus_tag="AC1LW8_002013"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIP4773822.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTLRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATVSGLSNKQLESLKATKPARIKLKWTPQIMHQIRVLR
                     ELEFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(6149..7627)
                     /gene="menE"
                     /locus_tag="AC1LW8_002014"
     CDS             complement(6149..7627)
                     /gene="menE"
                     /locus_tag="AC1LW8_002014"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HIP4773823.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMKNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITMNGLLDNTMDIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTIIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATLIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(7786..8235)
                     /locus_tag="AC1LW8_002015"
                     /pseudo
     CDS             complement(7786..8235)
                     /locus_tag="AC1LW8_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
BASE COUNT         2548 a         1625 c         1299 g         2764 t
ORIGIN      
        1 ttcgatacat attatcattt atataccgaa aatcttttat ttagctatat tcaattcatc
       61 ttattatttt actgcgtctt ttaattcttc cactttgtct aatttttccc atgggaataa
      121 gacatctgta cgtccaaaat gaccataagc agcagtttgt ttgtaaatcg gttgtttcaa
      181 atcaagcatt ttaataatac ctgcaggtct taggtcaaag tgttttctaa ctgcttcgac
      241 aagttgccct tcagaaactt tacctgttcc aaatgtatca attgcaattg acactggttc
      301 tgcaacacca atcgcatatg ccaattgtac ttcacattga tctgctaaac ctgctgcaac
      361 aatattttta gccacataac gtgcagcgta tgcagctgaa cggtctactt ttgtaggatc
      421 cttaccactg aagcatccgc caccatgacg tgcatagcca ccgtacgtat caacaatgat
      481 tttacgtcct gttaatcctg catcaccttg aggtccaccg attacaaagc gtcctgtagg
      541 attgatgtag aatttagttt gttcattaat caagttttct ggaacagttg gataaatgac
      601 atgtgcttta atgtcttctt gaatttgttc aagtgtcaca tcctcagcat gttgtgttga
      661 tacgacaatc gtatcaatac gtactgggtt atcattttca tcatattcaa cagtgacctg
      721 aactttaccg tctggtcgta aataatttaa cgtaccatct ttacgcacat ctgataaacg
      781 ttttgccaat tgatgtgata aataaattgc tagaggcata tacgtctctg tttcattcgt
      841 tgcgtaacca aacattaaac cttggtcacc tgcacctgtt gcttcaattt cttcttcgct
      901 atctttatca cgatactcta atgctttatc cacgccttgt gcaatgtcag gtgattgttc
      961 atcaatcgca gttaaaattg ccattgtttc ataatcataa ccatattttg ctcttgtgta
     1021 tccaatttct ttaattgttt ctctaacaac tttcggaata tcaacatatg ttgttgtaga
     1081 aatttcgccg gcgatcaatg ccatacctgt tgtaacagtt gtttcacaag ctacacgtgc
     1141 atttgggtcg tcttttaaaa tagcatctaa tattgcatct gacacttggt cagcgatttt
     1201 atctgggtgt ccttctgtaa cagactctga agtaaataat cgtttgttat ttaacatagt
     1261 ttgctccttt aaatttatat tacgaaaatt ctctctctgt gagctaaata aaaaagacct
     1321 tctaactatt aatatagaga gaaggcctaa tacgtccatt cgctcttatc gttcagacct
     1381 atttgtctgc aaacggtttg gcacctttct tttataaaaa aagaggttgc tgggtttcat
     1441 tgggtccatg tccctccacc actcaggata agagaatccg ttaaaaataa tagtacctaa
     1501 ttaatgaatt aatgtcaatt tttcacaaat aaatttacag taaaatattg tagattaatt
     1561 atgttaatgt gttatactaa ttaaatgtaa aggcttacat ttaaattatc gctttggagg
     1621 gatttaggat gtcagtagac acatacactg aaacaactaa aattgacaaa ttactgaaaa
     1681 aaccaacgtc acattttcaa ctttcgacga cacaacttta taataaaatc ttagacaata
     1741 acgaaggggt attaacagaa cttggtgctg ttaatgcaag tactggaaaa tatactggtc
     1801 gttcgcctaa agacaaattt tttgtctctg aaccttcata tagagataac attgattggg
     1861 gagaaattaa tcaacctatc gatgaagaaa ctttcttgaa gttataccat aaagtactag
     1921 actatttaga taaaaaagat gaactatacg tatttaaagg ctacgctggt agcgataaag
     1981 atacaatgtt aaaacttaca gtcatcaatg aattagcatg gcataattta tttgctaaaa
     2041 atatgtttat tagacctgaa tcaaaagaag aagctacaaa gattaaacct aacttcacta
     2101 tcgtttctgc cccacatttt aaagcagatc cagaagttga tggtactaaa tctgaaacct
     2161 ttgtcattat ttcatttaaa cacaaagtca ttttaatcgg cggtactgaa tacgctggtg
     2221 aaatgaaaaa aggtatcttc tctgtaatga attatctctt accgatgcaa gatattatga
     2281 gcatgcattg ctcagcaaac gttggtgaaa aaggcgatgt tgcattattc tttggtctat
     2341 ctggcactgg taaaacaacc ttatcggctg atccacaccg aaaactaatc ggtgatgatg
     2401 aacacggctg gaataaaaac ggggtcttta atatcgaagg tggctgctat gcaaaagcaa
     2461 ttaatctttc caaagaaaaa gaaccacaga tttttgacgc aatcaaatat ggtgcgattt
     2521 tagagaacac tgtagttgca gaagatggtt cagtggactt tgaagacaat cgttatacag
     2581 aaaacactcg tgccgcttat ccaattaatc acattgacaa tattgtagta ccatcaaaag
     2641 cagcacatcc aaatacaatt attttcttaa ctgcggatgc atttggtgtt attccaccga
     2701 tttcaaagtt aaataaagac caagcaatgt atcatttctt gagtggtttc acttctaaat
     2761 tagctggtac agagcgtggt gtgacagaac ctgaaccatc attctcaaca tgtttcggag
     2821 caccgttctt cccgttacac cctactgttt acgctgattt attaggtgaa cttatcgatt
     2881 tacatgatgt tgatgtttat cttgttaata ctggatggac tggcggaaaa tatggtgtag
     2941 gacgtagaat cagcttacat tacacacgtc aaatggtaaa ccaagcgatt tctggcaaat
     3001 tgaaaaatgc agaatataca aaagatagta cgtttggttt aagcattcct gtaaaaattg
     3061 aagatgtacc gaaaacaatt ttaaatccaa ttaatgcttg gagcgacaaa gagaaatata
     3121 aagcacaagc agaagattta attcaacgtt ttgaaaagaa cttcgaaaaa tttggtgaaa
     3181 aagttgaaca tattgctgaa aaaggtagct tcaacaaata aatttgaata ctaattaaag
     3241 agccaaccgt tgttatagtc taacaatggt tggctcctct tattttatgt gctaaaaatt
     3301 tataggcaat tttattacaa caatgcacat ttaaggtgac cttcatgcca aactcgcatc
     3361 actcatttaa tggaagcagc acgtcttcac ataaagtacc ggttccttac gctatgcatg
     3421 ttgccctact ttgcataaag tacttatcgt taactcaacg caagttgttc ctattcacaa
     3481 aaagcacata cagttatctg catggagcac attttcattg aaagcgcata cccctcattc
     3541 aacgtatatc acacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact
     3601 agctatgcaa ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
     3661 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
     3721 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
     3781 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
     3841 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
     3901 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
     3961 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
     4021 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
     4081 catttaaatc gccacggtaa aattcgtctt taccttcact cccattgtta cctcggtagt
     4141 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
     4201 gtcctacttg acctttgcca cctcgcaaat atactacaat gcgatttact tcatgatgtg
     4261 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
     4321 gatgcgtaaa agattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     4381 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     4441 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     4501 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     4561 tcaatggtat attgtgctat gtaatgaata tttttaactt tggcgcctgt ttcttcatat
     4621 aattcacggg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     4681 tcaatacccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     4741 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     4801 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     4861 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     4921 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     4981 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     5041 ccgtcgtatt ttaaaatgtc atccgcttca taaaggtggc tttgaccctg ttccgttaaa
     5101 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     5161 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     5221 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     5281 tttacgagca agcatcgctg taaaataacg gcttaaacca tattcgtaca tgccgccaat
     5341 aaccactttt acaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     5401 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     5461 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccac
     5521 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     5581 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     5641 caattgaaag tgaaactcta attcccgtaa cactctaatt tgatgcatga tttgaggtgt
     5701 ccattttaat ttaattctgg ccggcttagt tgcttttaat gactctagtt gtttatttga
     5761 taagccgctc actgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     5821 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     5881 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     5941 aattacatgt ttcactgaag caattgtttc atgatcatac caatctgttt gaaaagcgtt
     6001 acattccccg aaatatgcat ttcctttatc atcaatcaat tcgataaaca aacaatcacg
     6061 aagtgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     6121 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     6181 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     6241 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     6301 caaaatataa tttaggcact tgtccccaag tatcatcagg atgccctaca catactgcgt
     6361 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     6421 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     6481 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     6541 gatacaaata tccattcatc acattggcac ctttaatcat taattctcca tgaccttctt
     6601 tattaggatt tttaatttta acgtctacat tggcacttgg catccctaca gtgtcaggac
     6661 gtgcatgcaa catttccggt gttgctgtta aaaattgtga acatgtctca gtcataccaa
     6721 atgaattata aattggcagg ttatattgta atgccgtctc tatcaaagtg gcagataatt
     6781 tagcaccgcc gagtaatatt ttttgcaaat tataaggttc atgtaaacct tgttgcataa
     6841 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat gcgttcattt ttaattatcg
     6901 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaccct tcaataacag
     6961 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     7021 tatcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     7081 acgtttgcgg caccgctttt tgagggccag ttgtccctga tgtaaacata atcgatgcaa
     7141 tgtcatctaa attaaatgaa gtatttaata tgttggacgg cgactctttc ggcaccacag
     7201 tttcattcga tgtatcatat tggatatcca ttgtgttgtc caacaaaccg ttcattgtaa
     7261 tatccgttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgcaattcca
     7321 gtggcaaggt acaaaaaatc aattgtacat cgattgacct catctgattc ttcatctcat
     7381 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa caagcatgta
     7441 ttaaaatgat cgattgaatc gaattatcta tgtatagccc gacacgagat tgttgataag
     7501 ccttgagtct tttagccaat agactcgctt cacagtataa attttgataa gtataagatt
     7561 cttgaccgtc tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa
     7621 agtccatacg ctattcctcc aaattcatct accttataat tataacgatt ttatgacatt
     7681 ctagcagtgg ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt
     7741 gttataatgc tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt
     7801 taattttagt gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc
     7861 tgcaatgtca attggtatac gattcatgtc ttgtaataca cttaaatgga atacttcatc
     7921 atctaaattt tcaatgagat atacataata tgttatctta tcctttttat attttaacgt
     7981 tttccaaaag tcctacttgc aattcaatac attatccgga atatattcaa taaataagta
     8041 acgtttgctg cctactttgt ctacgaaata ttttacagtg cctttttcta tacctcttat
     8101 atgtgcatag tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc
     8161 aaatcgttgg cctactattt tattatttgt gccacaacta cttaaaaaaa tcagtagata
     8221 tagcattaaa catatt
//
LOCUS       DBNENW010000112         8062 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNENW010000112 DBNENW010000000
VERSION     DBNENW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.113"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(163..753)
                     /locus_tag="AC1LW8_002016"
     CDS             complement(163..753)
                     /locus_tag="AC1LW8_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIP4773824.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNATVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKAREMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(750..962)
                     /locus_tag="AC1LW8_002017"
     CDS             complement(750..962)
                     /locus_tag="AC1LW8_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIP4773825.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            1023..1569
                     /locus_tag="AC1LW8_002018"
                     /pseudo
     CDS             1023..1569
                     /locus_tag="AC1LW8_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1664..3280)
                     /gene="groL"
                     /locus_tag="AC1LW8_002019"
     CDS             complement(1664..3280)
                     /gene="groL"
                     /locus_tag="AC1LW8_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIP4773826.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(3356..3640)
                     /gene="groES"
                     /locus_tag="AC1LW8_002020"
     CDS             complement(3356..3640)
                     /gene="groES"
                     /locus_tag="AC1LW8_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIP4773827.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3815..4558
                     /gene="mroQ"
                     /locus_tag="AC1LW8_002021"
     CDS             3815..4558
                     /gene="mroQ"
                     /locus_tag="AC1LW8_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     MroQ"
                     /protein_id="HIP4773828.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(4583..5842)
                     /locus_tag="AC1LW8_002022"
     CDS             complement(4583..5842)
                     /locus_tag="AC1LW8_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrH family protein"
                     /protein_id="HIP4773829.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     DKPKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPN
                     ASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYG
                     EVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALIT
                     GEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCS
                     MMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            6039..6665
                     /locus_tag="AC1LW8_002023"
     CDS             6039..6665
                     /locus_tag="AC1LW8_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIP4773830.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            7026..7811
                     /locus_tag="AC1LW8_002024"
     CDS             7026..7811
                     /locus_tag="AC1LW8_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HIP4773831.1"
                     /translation="MKVQIYQLPIIFGDSSKNEAQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPIFKSIKLDLYK"
BASE COUNT         2505 a         1440 c         1233 g         2884 t
ORIGIN      
        1 aaatatcata cttttactcc tgaaaacctc cctaaatcat caatatgaaa atcagcatta
       61 ggatattgcg tttacaactc ttttaaatct cactcattct tctcatcgtc ttaaccgata
      121 ctatcaataa atactggcaa caccataaaa tatataccca atttataaaa acctaattga
      181 ctcataatct tctaacggta tatcatccac aatcacagta tgattaggat tatcgttaga
      241 tacatctttc actgccttat ctaacccctc atcacctccg tcccattcac caatattaat
      301 gaatataggt acattaccgt ttatatcatg cttatctgta aataatttat gatatttacc
      361 caacatttca cgagctttta aacgatcact aggttttatt ggtacctcta tcagttcaac
      421 atgttcatta tagactaatt gtactttgcc actttgtgga ttttctttat attctccacg
      481 cttgactaca acttctttcg tttctgtttc atcaccgact gtcgcattcg ttagcacatg
      541 taataactct tttgcggtta atacattctc atctataatc ttatcttttt gttcttgtat
      601 atattgcttg atgtgcggct ttttcaataa cctacacgct gtcacatgtg cgctatttgc
      661 gttatatcct gcttttatgg cactttgtgt tacattcagt gttctaatat actcattcac
      721 aaaacgtgct tgctttgcag ttaactcact cattttatca cccccacaat tttatctaat
      781 atggtttcat accataatat tacagattgt tctgaacaat ctaaggcact actaatatct
      841 tgataactaa gtccttgtat aagggagtca aaaatataaa actctttatc ggtcgctaat
      901 ctgtcaacaa tcatttctat gtgattcttt ataatatgat cattgacatt atcgtctgtc
      961 atcaattcgt cagaatcttc atcacctatt aaaaagaaat catcagtatt tatttcatca
     1021 tcgccccgtt aactagcttt gaagtcttta gcacacttgc atataccggc tgtcgtgctg
     1081 gcgagatact agcattgaag tggtctgata ttgattttga aaacaacacg attagtatta
     1141 ctaaaacata ttacaatcca aataataaca agaagaaatt tcagatactt ccccctaaaa
     1201 ctgaaagttc tatcggtaag atttccgttg acccaaatgt aataaaggtg ttgcgtgatt
     1261 ataagataaa cgttcaaaat aattggaaaa acgaattata taatgataac cattgtaatg
     1321 aaaaaactat caatgtggat acaaaccatt atgtcacgta ccagcatact caaaaatatc
     1381 tctacgcact cattcagata cacacattgc gcattgctca tagaatcagg tgtacacatt
     1441 aaagaaatac aagaacgatt acggcataaa gatatcaata ccactatgaa tatctatgct
     1501 aaaatcacaa attcatacaa aaaagacgcc tcccataagt ttagtcacca aatggaagac
     1561 gtctcgtaat taaaatatat ttgcgtgcat tctaatttat acttagaatg aatcatactc
     1621 gtgcataatg taattttcta gttagtcaaa actataaaca gttttacatc attcctggca
     1681 tgccacccat gttaggttgg tcattatttt tttctggaat tgatgctaca accgcttcag
     1741 tcgttaagaa cattgctgca acacttgctg catgttgtaa tgctgagcgt gttactttag
     1801 ttggatcaac gatacctgct tctaacatat taacccactc gtttgtagca gcgttaaaac
     1861 caacacctgg ttctgcattt ttcaagcgtt caacgatgac agagccttct aatcctgcat
     1921 tttcagcaat ttggcgaact ggtgcagtta atgctttaag tacaatattc acacctgttt
     1981 caatgtcgcc ttcagcttca atttcactta ctttttggta aacatttact agtgcagtac
     2041 caccacctgc aacaatacct tcttcaactg ctgcacgtgt agagtttaaa gcatcttcaa
     2101 tacgtaattt acgttcttta agttctgttt cacttgctgc acctaccttg ataactgcaa
     2161 caccgcctgc taatttagct aagcgctctt gtaatttttc acgatcaaag tcagattcag
     2221 tttcttcaat ttgagatttc aattggctaa cacgtgcatc aatgctgttt tcgtcaccgt
     2281 caccatcaac aacagtggta ttatctttag ttacttctac tttacttgca gtacctaaca
     2341 tatcaattga tgcatctttt aaatctaagc ctaaatcatc agtaatcact tgcgcaccag
     2401 ttaaaatagc taaatcttca agcatcgctt ttctacgatc accaaaacca ggtgctttta
     2461 ctgcaacagc tgtaaatgtg ccacgcatac ggtttagcac gatatttgtt aatgcatcgc
     2521 cttcaacttc atcagctaca attaagattg gacgattaga ttgaaccact tgttctaata
     2581 aaggtaagat atcttggaaa gacgagattt tcttatctgt tactaaaatg tatgggcgtt
     2641 ctaattcagc aaccatttta tctgaatcag taaccatata cggtgattga taaccacgat
     2701 caaattgcat accttcaacc acttctagtt cagtgtttag tccatttgat tcttcaattg
     2761 taatgacacc atcgttacct actttttcca tagcttcaga aatataacgt ccaatttctt
     2821 catctgctgc tgaaatcgca cctacttgcg caatttcatt tttattttca actttttgag
     2881 aattttcatg taacgcttca acagcaactt taactgcttt gtcgatacct tgtcgtaaac
     2941 caactgggtt cgcaccactt gtaacatttt tcaagccttc ttgaatcatt gcttgagcta
     3001 atactgttgc agttgtcgta ccgtcaccag caatttcatt tgtcttattt gcgacttctt
     3061 gaactagttt agctcccata ttttcatatg gatcttctaa ttcgatttct ttagcaatcg
     3121 ttacaccatc gttcgtaatt aaaggtgctg taaactcttt atctaataca acattacgtc
     3181 ctttaggacc aatcgttact ttaactgcat ttgcaagttg gtcaacacca cgtaacattg
     3241 cttgacgcgc atcttcagag aatttcaatt gtttaaccat aaatgataaa cctccatttg
     3301 tttagtcata tttcattttc gttcttttta atttatctat gaatttagtt ttgtattatt
     3361 caataactgc taaaatatct tcttcattta atactagata tgtttcatta tctcgtttaa
     3421 cttctgtacc agcatattgt tggaacacga cacggtcccc ttctttcact tcaggagtca
     3481 ctcttgtacc atcatttaat agacgtccag ttcctactgc aacgataacg ccttcgtttg
     3541 atttttcttt agcactatca gttaaaacaa taccactttt agttgtttgt tcttgttctt
     3601 ttttctcaat aatcacacga tttccaattg gttttagcat gattgttcct ccttaaaaaa
     3661 cctaaagttt agcacttaac attaaagagt gctaacatac atttataata atcaaatttg
     3721 gtcaaaattt caagtcataa ccttttaatt aagttttgta acttcaagtt atttacgata
     3781 aaataaatta taaacaaata tttggaggaa aattatgaca agattatggg catcattgct
     3841 aactgttatt atttatatat tgtctcaatt tttaccgctt ctcattgtaa aaaaattacc
     3901 atttgtacaa tatagtggca tagaactgac taaagcagtc atttacatac aacttgttct
     3961 atttttaatc gccgccacga cgattatttt aattaattta aaaattaaaa atccaacaaa
     4021 attagaatta gaagttaaag aacctaaaaa atatatcatt ccatgggcat tgcttggatt
     4081 tgcattggta atgatttatc aaatggtagt gagcattgta ttaacgcaaa tttatggtgg
     4141 acaacaagta agtcctaata cagaaaagct aattattatt gctcgaaaaa tacctatatt
     4201 tatcttcttt gtatctatta ttggtccttt attagaagaa tatgtattca gaaaagtaat
     4261 ctttggagaa ttatttaatg cgattaaagg taatcgtatc gtggcattta ttattgctac
     4321 aacagtaagt tcattaatat ttgcattagc acataatgat ttcaaattta ttccagttta
     4381 ttttggcatg ggtgtcattt tttcattagc atatgtttgg acaaaacggc ttgctgttcc
     4441 aattattatc catatgttac aaaacggatt tgtcgttata ttccaattac ttaatcctga
     4501 ggctttgaaa aaagccacgg aacaagcgaa ttttatatat cacattttta ttccataata
     4561 aaaaaaacga atcacacacg gattatcgtc gctgtgattc gtttttattt ttctttttat
     4621 ttcgctttac aaataagaat agcatgattg aaaataccac catcatagaa catacaattg
     4681 ctagccaact tttactatat gcagttgacg cacttttctt attatcttct tgttttttaa
     4741 ctactgtaaa atcgtctttt tcatgcggtt caaatgagcg ttgttttgaa tccggcttat
     4801 cttcgggctt tttcaaatct ggcattgatc caatttcacc agtaattaat gcgagtactt
     4861 gttcatctaa attacgatag tacctttctg atttcaatgg attgaaatat tggaatctaa
     4921 agtaattcga ttgctgttgt aatccattta aatatgcatt tccgctgaaa ttttgttttc
     4981 gaataatatt ataaatgtct tcatcagtaa cttctccata atctttgccc aacttattaa
     5041 tttgatttaa aatatacgga ttatacttgt aagccccatt tgccaaggct aaaaatcgtt
     5101 gggatacaaa atcattacca gtaggatttt gtgagtttcc ttgattccca tttggttgcc
     5161 attgaccttg attagatcca tctgaagcat ttgggttcca tgtcccccca tttttcgagc
     5221 caccagaatg attggaatcc ccaggttgat cgggatctgg tgacgggtta ggatttggct
     5281 tattagggtc tggttttgga ttcggatttg gctttggttt atctggatct ggttttggat
     5341 ccggatttgg ctttggttta tctgggtctg gttttggatc tggatttggt ttcgggttat
     5401 ctggatctgg ttttggatcc ggatttggct ttggtttatc tgggtctggt tttggatccg
     5461 gttttggttt cgggttatct ggatctggtt taggattcgg acttgggttt tggttatttg
     5521 ggtctggttt tgaatctgta cttgggtttt ggttgtttga gtctggcttc acattattcg
     5581 aatcaggatt agcatcacga ctattcgaac ctgttccttc attacccgtt tgatttgatt
     5641 cgtgaggtgt cccagtctca ttattattca tttcactatt tactgatggt gaagttatat
     5701 tatgattcaa attagtagtt ggtttttctc catttaagtt atcctttgct tcaactgtgt
     5761 gcgttgcaag ccctaaacta ctactactta aaattaaaat acttgttgaa agtaacattt
     5821 tcttaaacca atgatatgac atttcacgag acacctccct ttttcatgta agtattaatc
     5881 taagtataat aaatatgcgt tgaaaatgca tttatatttg gaatttgaaa atgaatatta
     5941 atattcttga aattttcgta aatattgctt tacaagtacg agtataaatt ttatacttag
     6001 tctattaagt attctttact aaagaaggag caataattat gggcttattt aaaaaagata
     6061 aaaaagcaat gacttttgac aatgcaatgg aagaaagacg ttcaatatat aatttgaaag
     6121 attcgatatc aattagcgat gacgaattag aatcaatgat tgcacatgca gttaaacatg
     6181 ttccttcatc atttaattct caatcaacta gaattgttct cttgttaaat gacaacaata
     6241 ataaattttg ggataacaca aaagcaattt taaaagaagt catgggagag aatcgtgatt
     6301 tcgagccaac tgaacaaaaa attgataact tcaaacattc atacggtaca attttgtttt
     6361 atgaagatca agatgttgta agtggtttac aagaacaaat gccaaactac tatgataatt
     6421 ttgcaatatg gtcaactcaa acaaatgcca tgcatcaatt tgcaatttgg actgcattag
     6481 ctactaaagg cataggtgct tcattacaac actataatcc acttgttgat gagatgactt
     6541 caaatgaatt taatattcct aaaagttgga aacttattgc tcaaatgcca tttggtgata
     6601 ttcgtgaagc tgctggcgaa aaaacattta atccagtgga agatcgtttc gtaataaaaa
     6661 aataatcaat atattgttag gaatgggaca gaaatgataa agaatcatta gttattcatt
     6721 atgtagtagt tcttacacat tagccgctgc taatgtgcac ttaagaatag gaatacatga
     6781 gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt tatgttagag
     6841 cccaccccaa cttgcattgt ctgtagaaat tggtgttcca atttctctat gttggggccc
     6901 catcaactac tgtcaatata acattgtaga gcctaggact ttgattttat gtcctgggct
     6961 cgttttttgc gtttcaacat aacttttagt aagttatata taagatctat caaggatgtg
     7021 atgttatgaa agtccaaatt tatcaattac cgattatttt tggagatagt tctaaaaatg
     7081 aagctcaaat aacgcaatgg ttcgaaaaaa atatgaatgc tgaagtagat gtagtcgttt
     7141 tacctgaaat gtggaataat ggctatgatt tagagcattt aaatgaaaaa gcagataata
     7201 atctaggaca aagtttttca tttattaaac atttagctga aaaatataaa gtcgatatcg
     7261 tagctgggtc agtttctaac attagaaact atcaaatatt taatacagct tttagtgtga
     7321 ataaaagtgg ccagctaatt aatgaatacg acaaagttca tttagttcct atgttaagag
     7381 agcatgaatt tttaactgct ggcgaaaacg tagctgaacc ctttcaattg tctgacggta
     7441 cttatgtgac ccaacttatc tgttatgatt tacgttttcc cgaattatta agatatcctg
     7501 ctcgcagtgg tgctaaaatt gcattttatg tggcgcaatg gcctatgtca cgtcttcaac
     7561 attggcattc attactaaag gcacgtgcaa ttgaaaataa tatgtttgtc attggaacta
     7621 atagcactgg attcgatggt aacacagaat atgctggtca ttcgatagtt ataaacccaa
     7681 atggagattt agttggagaa ttaaatgaat ccgcagatat tttgactgta gatttaaact
     7741 taaatgaagt agaacagcaa cgcgaaaata tacctatatt taaaagtatt aaattagatt
     7801 tgtataaata agaagcgccc gaaataataa ttaacacatg tcatgactaa acatagattt
     7861 atgagagatt tttcataagt ctatgtttat ttttttatag tagataactc agtaagaatc
     7921 catttcgccc tttgcaaatg aatgattttc ttaattaaat tgattaattt tttgaccttt
     7981 tccaacatta gacttattca tatattttaa cggtggatct cataatgagg attaactctt
     8041 cccttcttta ttacaataaa aa
//
LOCUS       DBNENW010000113         7971 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNENW010000113 DBNENW010000000
VERSION     DBNENW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.114"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..112)
                     /locus_tag="AC1LW8_002025"
     CDS             complement(<1..112)
                     /locus_tag="AC1LW8_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773832.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKI"
     gene            complement(322..771)
                     /gene="ndk"
                     /locus_tag="AC1LW8_002026"
     CDS             complement(322..771)
                     /gene="ndk"
                     /locus_tag="AC1LW8_002026"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIP4773833.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLDSAEREINLWFNENEITSYASPRDAWLYE"
     gene            complement(863..1822)
                     /locus_tag="AC1LW8_002027"
     CDS             complement(863..1822)
                     /locus_tag="AC1LW8_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP4773834.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSDRKEFEECIQIIRKSDS
                     INESKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            complement(1824..2549)
                     /locus_tag="AC1LW8_002028"
     CDS             complement(1824..2549)
                     /locus_tag="AC1LW8_002028"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HIP4773835.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            complement(2552..3124)
                     /locus_tag="AC1LW8_002029"
     CDS             complement(2552..3124)
                     /locus_tag="AC1LW8_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HIP4773836.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            complement(3555..3827)
                     /locus_tag="AC1LW8_002030"
     CDS             complement(3555..3827)
                     /locus_tag="AC1LW8_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIP4773837.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            complement(3998..4996)
                     /locus_tag="AC1LW8_002031"
     CDS             complement(3998..4996)
                     /locus_tag="AC1LW8_002031"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIP4773838.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            complement(5013..6323)
                     /gene="der"
                     /locus_tag="AC1LW8_002032"
     CDS             complement(5013..6323)
                     /gene="der"
                     /locus_tag="AC1LW8_002032"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIP4773839.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            complement(6545..7720)
                     /gene="rpsA"
                     /locus_tag="AC1LW8_002033"
     CDS             complement(6545..7720)
                     /gene="rpsA"
                     /locus_tag="AC1LW8_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIP4773840.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
BASE COUNT         2474 a         1532 c         1043 g         2922 t
ORIGIN      
        1 taatttttgt tataggatta agtggtttat ctaaaaatat agcaccatat agtaattcca
       61 ttaattttaa gaacattaaa atccccaaaa taatcgaaat aatattcttc attttaacaa
      121 ccccttctct ctgtttagtt tcatttgata aagaatattc ctctcttttt aatacgtgtt
      181 accaaaaaaa ctataaaatt tttcaagtaa tttactacaa cataaaaaac gggccttgtt
      241 atgtataatc ataatgacta accacataac aaaacccttt ctacctttat aaataaatcg
      301 taaaggttta cagtttaaat tttattcata taaccatgca tcacgtggtg aagcatagct
      361 agtaatttca ttttcattaa accatagatt aatttcacgt tcagcagaat ctaatgaatc
      421 tgaaccgtga atgatatttc taccaacagt taaacctaaa tcacctctaa ttgatcctgg
      481 tgaagcttct gaaggattgg tgctgccaat aatatgtcta gatacattaa ctgcatcttc
      541 accttcaact accattgcga acactggtgc tgatgtaata aatgaaatta aatcattata
      601 aaatggttta ccttggtgtt caccataatg tgtttcagca agttccattg gtacttgcat
      661 taatttacca ccgacaagtt ttagtccttt tctttcaatt cttgaaatta cttcaccaat
      721 tagatttctt tgtactgcat ctggtttaat cattaaaaat gtacgttcca ctttatgtat
      781 ccccctgtgt ttaataggtt tatgtatatt actattatat attaacaggt tggtaaagcg
      841 ctttcaatac ttttcataaa tactacgtgt ttcttgaacc catttttttc gtcaaactta
      901 aaagtagtga tttcggatgt ccatctggta actcagaaat caaatccaaa gctttactta
      961 aatacttcga acttactgct ttagattcat taatactgtc agactttcta ataatttgaa
     1021 tacattcttc aaattctttt cgatcgctgt cacgtcgtaa ttgctcaatt ttcaatttga
     1081 agtctgggtt tttacgcatt tctaataaaa tcggtaacgt aatatgacca ttaagcaaat
     1141 cacttccgac cggcttacct aatttctttt cggtacttgt gaagtctaat acatcatcaa
     1201 tgatttggaa gctcatacct atataatgac caatcatttt caattttcgt acagtctctt
     1261 tatcagattg agatgtaatt gcaccaactt cagttgatat ttgaattaac agtgctgttt
     1321 tgcgattgat acgtcgtaaa taattgataa ttgtctgttg actgttaaat tggtcttgaa
     1381 attggaaaag ttcccctcta caaacatcaa cgatagattc agatatcaat tgatgtacac
     1441 gattatcttt aacggccatt aagtgttcaa gtcctaatgc caataaaaaa ttcccagtta
     1501 aaatagcagt tgtctgatcc catttctttg atatggttaa cttgcctcga cgcttgtcgc
     1561 ttttatcaat aacgtcatca tgaacaagtg ttgccatatg aattaactct aatgcgactg
     1621 caacttgata cgtttgttca gacgtttgtt catctttgcc aaattggcta ctcagaatta
     1681 caaatgctgg gcgtactctt ttaccaccag aagacaataa gtgtaatgat gcctgttcta
     1741 atacagaatc tttacttttt attgcctttt caagtcgttg ttccactttc ttaatttcat
     1801 tgttcatgtt taactttgcc acgttaatca cctttggtat tatctttttc tttatagcca
     1861 aggtgcattg cagcaacgcc ccctgtaaaa ctacgtactc ttacatttat gaaacccgct
     1921 tcttcaaaca tgcgcttcaa ctcttctttt ccaggaaaat taaacgtaga ttgctgtaac
     1981 cattcatatt cttcttttga ttttgcaaat aatttcccaa aaataggcat aacaaattta
     2041 aagtataatg catacatttg tttaaagact ggcaaagttg gttggctcgt ttcaagacat
     2101 accaccatac cacctggttt aagtactcta ttcatttctt ttaacgcgac taaatagtct
     2161 ggcacattcc ttaatccaaa cccaattgtt acataatcaa aagaattgtc ttcaaacggc
     2221 aattccattg catcaccatg aacaagttta acattttcca ttgaagcagt tttttctttt
     2281 cctacttcta acatattctc actaaagtca ataccagtaa cttcacctgt tggtcctaca
     2341 gctttgctta atgcgattgt ccaatcacca gtaccacaac aaacatctaa tgctttcgtc
     2401 ccttttctaa cgcccatgtc tttcatgacg cgttttctcc atactttatg ctgctcaaaa
     2461 ctaataatat tatttaatct atcatatttt tttgaaatat tttgaaaaac gcgatgtact
     2521 tgctctttat ttgctttatt gtcagccatg cttaattacc tctacttttt aaataacttt
     2581 tttggatatc gtgtaagtaa tgctttactt cactttgatt atatttcttg aagtatgatg
     2641 gatagtaatc agacatatct tcaaataaat aattatatat ttccgactca tcaatattga
     2701 taccgaaatg agataacgta atatatggga aaagtgtttc aatttttact attgcttgag
     2761 aaatttcata atcatttaaa gcttgatgat gtaatgaaga tttcaattca ttaatttcaa
     2821 caattgcttt actaatttca ttttgaaatg ataaatcatt gatttctgct agtagcgtat
     2881 aaaaatgtgc actaattaaa tctccaatca aaatggaatg tttagacaaa tgattatatg
     2941 taatgtcatc aaggtgtctc attgatgtgt caattgtgag gcatgctact ttggcaacat
     3001 ctggaatatc atatgaatca agtaacttac ctaaccgatg attaatatta atggattcat
     3061 attctgatac accctttaat ctttcttcta tttgtctttc caatttgcta acagttgttt
     3121 ccatgtctac acctcacgat attatcacta ttcatattaa cattatatgt aagaaattaa
     3181 aatcttttga agcattaaga ttacttatca tttttaaatt tcaatttaaa ctaacagtaa
     3241 tttatgtagc ttttgtaatt ctcataataa cctttacttc atttacatta ttcatctttt
     3301 taaataacat aaaatactat agtagacatg gttacctttt aagtaaattc aacgctaaca
     3361 attaatctca ttattcatat gtatattttg tagttgctat ttttatcaaa actattatgt
     3421 catgtaaaat tttgaacgct taaacaatga atgttccaaa gattaaaaac ttagaagaac
     3481 attacaattt atgtacaggt tgtaagtatt aaaagcaaaa taaaaagccc ttattcaggg
     3541 ctttttaagt aaaattattt tacagcatct tttaatgctt taccagcttt gaatgctgga
     3601 actttacttg ctgggatatc aatttcttta ccagtttgag ggttacgacc tttacgtgca
     3661 gcacgttcac gtacctcaaa gttaccgaaa ccaattaatt gtactttttc acctttagca
     3721 agtgagtttt ggattgattc gaatacagca tctactgctg aaccagcttc ttttttagtt
     3781 aaatcagctt gctctgcaac tgcattgatt aaatctgttt tgttcattag acattcacct
     3841 cctgaggttt gataatgata tgtttatatc attatggtaa aactttaaca caagcattac
     3901 ataaatcgca atcatttatg tggcaaaaac gttgaattta agccatttct attcatttta
     3961 cgtaattatt agccgtatat gtgtaataat acacatttta ttcagatttt ttatcgcgct
     4021 ccattaaatc ttttacgcat tcttttactg agatattttc aaataatact ctatataatg
     4081 catttgtaat tggcatatcc acattttttt ctttagctaa atgataaact gatttagttg
     4141 tataaatacc ttcaacgacc atattcattt cagataatgc ttgatccatt gattcacctt
     4201 gtccaagttt atatcctaat gtgaaatttc gagaatgtgt tgatgtgcaa gtaacgatta
     4261 agtcaccgat accacctaaa cctagaaatg tcataggatc ggcacctaac ttttcaccta
     4321 atctactaat ttccgctaaa ccacgagtca ttaatgcagc ttttgcatta tcaccgtagc
     4381 caattccagc tacgatacca cttgctactg cgatgatatt tttcaatgca ccaccaagtt
     4441 caacaccaat caagtcatca ttcgtgtaca cacgcaaata atcattcata aataaatctt
     4501 gcgttaattt acttacactt ttatcttttg atgaagcagc aactgtagtt ggttgcttga
     4561 ctacaacttc ttccgcatga cttggccctg acaacacgcc aatacctgca ttatattcag
     4621 gtgaaataga atcttcaatc atttctgaca cacgtttaaa cgtcccattt tcaatacctt
     4681 tagcaacatg tataaaagtc tttttagagg tcagcttatc attaatttga gaagcaactt
     4741 ctcgcattgc tttagtaggt aaagccatta agtaaatatc tgcaaattga attgctttgg
     4801 tcatatctga agtagcgatg atgttaacat ctaatttcgc gtattttaaa tactttttat
     4861 ttgtatgaca tgtatttaat tcatcaacag catcttgatt tttaccccac atcaaaacat
     4921 catgtccatt ttctgcaaga acattggcaa gggctgtccc aaaacttccc ataccaaaaa
     4981 cggtaatttt agtcattgtt atcccccaat cgttaatttc tctttcgagc tataatatga
     5041 attggtgtac cttcaaaacc aaaagcggca cggatttgat tctctaaata gcgtttataa
     5101 gaaaaatgca ttaattctac atcattaaca aatacaacaa atgtcggtgg ttctatagca
     5161 acttgtgttg cataaaagac attcaaacgt ctacctttgt ctgttggtgt agggttcatg
     5221 gaaattgcat cagtaacaac ttcatttaaa gttgaacttt gaacacgttt tttatggttt
     5281 tcacttgctt cattgatgta agggaataat gtacgtaatc ttgtgcgttc tttagcagac
     5341 acaaaagcaa tttgtgcata atctaaaaat tggaattctt tacgtacttc atcttcaaat
     5401 ttcttcatcg ttttactatc tttttccaca gtatcccatt tatttacgac aatcacgact
     5461 gctttacctt gttcatgtgc atatcctgca acacgtttat cttgttcaat gatgccttgt
     5521 tctgcatcta tgaccactaa aacaacattt gaacgttcaa tcgcttttaa agctcttaat
     5581 actgaatatt tctcagttga ttcatatact tttccttttt tacgcatacc agcagtatcg
     5641 attaaaacat aatcttgtcc atcataacta tactctgtat caatagcgtc tctcgttgtc
     5701 cctgcaacat tagaaacgat aacgcgatct tcacctaaaa tagcatttac taaacttgat
     5761 ttacctacgt ttggtcgtcc aataatggat agtcgaattg tatcttcatc ataaggatct
     5821 tcttcctctt caccaaaatg agaaacaact gcatctaaca agtcaccaag acctaaacca
     5881 tgtgaccctg atatcggata cggttcacca aatcctaatg aatagaaatc atacacgtct
     5941 gtacgcattt ccatattatc tactttgtta accgctaata cgaccggttt tttagatttg
     6001 tataaaattt gagcgaccat ttcatcgctt tgtgtcaatc cttcacgcac gttaaccata
     6061 aaaataataa catccgcttc atctatggcg atttctgcct gcgctctaat ttgtgtttgg
     6121 aatggtgcat caccaatttc aataccacct gtatcaataa tattgaaatc atgtgttaac
     6181 cattcacctg aagaataaat acgatctcgt gttacacctg gcgtgtcttc cacaatcgaa
     6241 acacgttctc caactattct attaaaaatt gtagatttac ctacattagg cctacctaca
     6301 atagctacta taggtttagt cataagctaa cttcctctct ttcttatatc tttttaataa
     6361 tatcacatga gactgtgtat tcaaaatgag tttattactg taatgataaa aatcagcact
     6421 taaaacttta tctttttaaa cacttagtta aatttgtcca cgttatcatt ccgagtatat
     6481 tttgttacat aaaaatactc cacatgcaat cataaacatg tggagttgac tattaaatat
     6541 taaattatag tttaagattt ttaagtttat caccaatcat atcgccaatt gttggattat
     6601 cttcttcttc gttttctaag tacgccttag tcgtagaagg atcactttca acaacatctt
     6661 cgtttggtaa tgttgcttta atagatagtg atactctttc attctcttca tcaataccta
     6721 atattttaac atttacttgt tgaccaggtt ctaacacttc acctggcgta ccaatgtgtt
     6781 tgtgtgcaat ttcagaaata tgtacaagtc cttgtacacc tggtgcaatt tcaacaaatg
     6841 caccaaagtt tgccaatctt actacgacac cttcaatgac atcattttcg tggaattgac
     6901 ctttaatatt ttcgaaaggt gttggtaacg tatctttgat tgataatgaa atacgttctg
     6961 tatctctatc aatagattta attttaactt taacatcttg accaattgaa actacttctt
     7021 ctggtgtttg aacatgttcg tgagaaagtt cagatacatg cactaaacca tcaacaccgc
     7081 caatgtctat aaatgcacca aattgagtta aacgcgctac tttaccatca ataacatcgc
     7141 cttcatttaa agattgtaat aattgatctt ttttagcatc gttttcttct tgttcaactg
     7201 ctttacggct taaaatgact ctattatttt caggatccaa ttcttcaact ttaatacgaa
     7261 ttgtttgtcc atcaaacaca gagaaatcct caatgaagtc tgttgaaatt agtgaagccg
     7321 gaacaaaacc tctttgtcct acatcaacaa ccaaaccacc tttaactact tctgttactt
     7381 tcgcttcgat gatttcatta ttatctaatt tttcttgtaa ataactataa gacttctcag
     7441 tttcaagttg tcttctagat aagatgtaag ctccagtttc attttcttca tcaaactcaa
     7501 ctttagtgac atatgcttca acttcgtcgc cctcttttac aacttcactt gggctatcaa
     7561 tatgatgcgt agatagttga ctaataggaa taatcccatt aaatttacca ccgttgatat
     7621 gaacaacaac ttgcttgtct tcaacttgtt gtacctcgcc agtgactttg tcaccttctt
     7681 taatatcgtt aatcattgat tcattgaatt cttcagtcat cttgtttgcc tccttataca
     7741 ctacatattt ataacatctt ataaactgac agaattgtca agaaattcta cctcgattac
     7801 attttgcgaa atagtcatat acataaatat gtcactttat catattcaag tcgtcatatc
     7861 gataaatatt tattacaacc atttttgtat caaagtaata cagtttgcta tgtttataca
     7921 tattttttgt ataaaaatta gcatagctta ctattttgtt ttataagtct g
//
LOCUS       DBNENW010000114         7900 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNENW010000114 DBNENW010000000
VERSION     DBNENW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.115"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            331..732
                     /locus_tag="AC1LW8_002034"
     CDS             331..732
                     /locus_tag="AC1LW8_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4773841.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(995..2692)
                     /locus_tag="AC1LW8_002035"
     CDS             complement(995..2692)
                     /locus_tag="AC1LW8_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIP4773842.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIKSIKQIGNDRRIEIE
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            2967..3590
                     /gene="gmk"
                     /locus_tag="AC1LW8_002036"
     CDS             2967..3590
                     /gene="gmk"
                     /locus_tag="AC1LW8_002036"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIP4773843.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            3590..3808
                     /gene="rpoZ"
                     /locus_tag="AC1LW8_002037"
     CDS             3590..3808
                     /gene="rpoZ"
                     /locus_tag="AC1LW8_002037"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIP4773844.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            4024..5223
                     /gene="coaBC"
                     /locus_tag="AC1LW8_002038"
     CDS             4024..5223
                     /gene="coaBC"
                     /locus_tag="AC1LW8_002038"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIP4773845.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            5223..7631
                     /gene="priA"
                     /locus_tag="AC1LW8_002039"
     CDS             5223..7631
                     /gene="priA"
                     /locus_tag="AC1LW8_002039"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIP4773846.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2742 a         1145 c         1441 g         2572 t
ORIGIN      
        1 ctttattaaa atccaaccaa ttaagaatac gagtaatagg actaatactg gaatcacata
       61 atgtaacata acgtccctcc tttaacttaa ttttaattgt aatcaaattt gacaataagt
      121 caaaacatta atacctatga taagtatcat ttattaacat atgtatcata tttttaatct
      181 tgcgtaattt ttatcgttaa ctatgtgatt taatcaacag gccaataaga cgtttgtcat
      241 tcgtgcacat aacggtacga cgcgcttttg tcattttaat tatgttaaga taatagtagt
      301 tatagaagtt caatctatag gaggcatagc atggatattc caaaaatcac gacattttta
      361 atgtttaata accaagctga agaagctgtt aaactataca caagcttatt tgaagatagt
      421 gagattataa caatggctaa gtatggtgaa aatggacctg gtgatcccgg gactgtacaa
      481 cactcaatat ttacattaaa tggacaagta ttcatggcga ttgatgctaa tagtggcaca
      541 gaattaccaa tgaatcctgc gatttcatta tttgttacag taaaagatac tattgaaatg
      601 gaacgactat ttaatggatt aaaagatgaa ggtgccattt taatgccaaa aacgaatatg
      661 ccaccataca gagagtttgc ttgggttcaa gataagtttg gagtaagttt tcaattagca
      721 ttacctgagt aaaaggattc gcacagcgtt gaatgataaa gaaacacttt ttcttatgca
      781 tgcgttttac ctatgtattt ctattttaag tacacattag cattttagtt tcactcattt
      841 ttaaaatcac aaagataatt tgtaacttaa atgatgatac gttatttaaa aacacgatac
      901 ttcgtttcaa tgaacgcatt aaataataaa taaacacctc accataagaa aggactactt
      961 tcttttgtga ggtgtttatt tgtcgacaac ttgattatga ttgtttcatt ttttgaataa
     1021 gttcataatc aggtgtagca tacaaagttt tttgattgtc atatgttaca aacccaggct
     1081 ttgcacctga tggtttgtgc acatttttaa ttaatgtgta atcaacaggt atttgtccag
     1141 aattaccagc ttttgaaaag tatcctgcta acatagccgc ttccttgatt gtcgtatcac
     1201 ttggtgcatc attaaatata acgacatgtg aaccaggaat atcttttgtg tgtaaccatg
     1261 tgtgagtttt tttagctttt ttatttgtta aataatcatt ttgcttgtta ttcttgccaa
     1321 catatatatc gtcgccatca gttgatacat aatgttgtaa ttgaatctgc gctttctttt
     1381 tcttagtttg atttttacgc tgtttcataa agccttgttc tgctaattca tctctaattt
     1441 catcaatgtc atggacagaa atatgatgta attgttgttc gattgttgaa aaataatcta
     1501 tattgtcttt cgtcaattga atttgatgtt gtaattcacg ttctctcgtt ttcatacgat
     1561 tatattgttt ataataatat tgagcatttg ctgatgggga ttttgtagga tttaaaggaa
     1621 tgacaacttc ttcattcgta taataattca atgccgtcac ttctttatcg ccttgcttaa
     1681 ttcgatatat attagcagtg atcaattcac catataactg ttcagtatct ttatttttag
     1741 actgctcata ttcttcaatc aacttcgcta atttattttg atatttgtgc aactgctgtt
     1801 gaacaaatcg aactaaatca ttcgcacgtt gtttaacgcg ttcacgttcg ccacgcgcat
     1861 cataaaaacg atcaagtaaa tcatttaatg aatcgtatgt aactgtatca tcattaaatt
     1921 gatttaactt tataaaatag aaatcctctt tacctgtttc atgattttta tgaaaaatag
     1981 gagtaggtgg taacttggtt tctgccatta cttcgtcaaa tgcttctggt aatgttgatg
     2041 aagtcataaa ttgacgacga ctaacaattt cattcgtaat taaagggcta aatccttcaa
     2101 actgattcaa taattgttta gcaatattac ctgcgttaaa atcgatatat ttcaacacct
     2161 ctgcacctgt aatatcatac ggatttattt tgtgctgagt aggtggtgct tcataattaa
     2221 atcctggcat tactgtacga tagtgattcg tatttggtgt taagtgttta aatccttcaa
     2281 ttattttgcg attttcatct actaaaatta agttactatg tttacccata atctcaagga
     2341 tgacagtgcg gtaaatagta tcgccaattt catctttact ctttatttcg atttcaatgc
     2401 gacgatcatt accaatttgc ttaatcgatt tgataatacc accttctaag tgttttctaa
     2461 aaacacgcgc aaacatgggt ggattaaatg gattatcata ttttttagta gttaattgta
     2521 atcttgaaaa gtttggatgg attgacaata acaattgatg gttttgtcta ttttgacgta
     2581 caaccattag tatcgtgtca ttatcaggtt gattgatttt gtgaacgcgt cctgttgtta
     2641 aaaattgtag agactcaacc attttctttg taaataagcc atcataagcc ataatttatc
     2701 ttccaccttc ttcatatcat tcgtttcatt ttaacatgat tgtaactttt cggtatagtt
     2761 attgaaccat acattatgcc ttacatttta cattatctaa aagtttgcaa ttcaatttat
     2821 ttaattaaaa ttaatcgtgc aatataacga tattgttaga cttaatagaa attatggcat
     2881 gcaatttcaa atatgctata caatataaag aacaatgtga tatcatattt aaataataga
     2941 agattagctt agagaggtcg taaggcatgg ataatgaaaa aggattgtta atcgttttat
     3001 caggaccatc tggagtaggt aaaggtactg ttagaaaacg aatatttgaa gatccaagta
     3061 catcatataa gtattctatt tcaatgacaa cacgtcaaat gcgtgaaggt gaagttgatg
     3121 gcgtagatta cttttttaaa actagggatg cgtttgaagc tttaatcaaa gatgaccaat
     3181 ttatagaata tgctgaatat gtaggcaact attatggtac accagttcaa tatgttaaag
     3241 atacaatgga cgaaggtcat gatgtatttt tagaaattga agtagaaggt gcaaagcaag
     3301 ttagaaagaa atttccagat gcgctattta ttttcttagc acctccaagt ttagaacact
     3361 tgagagagcg attagtaggt agaggaacag aatctgatga gaaaatacaa agtcgtatta
     3421 acgaagcgcg taaagaagtt gaaatgatga atttatacga ttacgttgta gttaatgatg
     3481 aagtagaact tgcgaagaat agaattcaat gtattgtaga agctgagcac ttaaaaagag
     3541 agcgcgtaga agctaagtat agaaaaatga ttttggaggc taaaaaataa tgttaaatcc
     3601 acctttaaac caattaacgt cacaaattaa atcaaagtat ttaattgcaa caactgcagc
     3661 gaaaagagcg cgtgaaattg atgaacaacc tgaaactgaa ttattaagtg aatatcattc
     3721 atttaaacca gttggtagag cgttagaaga aattgctgac ggtaaaattc gccctgttat
     3781 ttcaagtgat tattatggta aagaatagtt tcgaccatta aaaatatgtg aagctagacg
     3841 tacatcaaat acgtcaaggc atatccaaat caaacgcacc tatgtaggtg tgttttttta
     3901 gtttatccaa tgattgatgt tataataata ctaaatttgt atctataaaa aagtaatgag
     3961 catttgtgcg catatgatga tgtaaagcgt aaatgtcata gtgatattta agggagattg
     4021 aatatgaaga aaatattatt agccgttaca ggtggcattg cggcatataa agcaattgat
     4081 ttgacaagta agttaacaca atctgggtat gaagttcgcg ttatgttaac gaatcacgca
     4141 caaaaatttg tgacaccatt agcatttcaa gcaataagtc gaaatgctgt ttatacagat
     4201 acttttatag aagaaaatcc ttcagaaata cagcatattg cattaggtga ttgggcagat
     4261 gcaatcattg ttgcacctgc aacggcaaat acaattgcaa aattgagtgt aggtattgct
     4321 gatgatttgg tgacatcaac gttgctagca acagagacac cgaaatttat tgcgcctgct
     4381 atgaatgtgc atatgtatga aaataaacgt acgcagcaaa atattaatat tttaaaagaa
     4441 gatgggtatc attttatcga accaggaagc ggatttctag catgtggtta tgttgctaaa
     4501 ggacgtatgg aagaaccgct tcaaatcgtt tctgtaattg atgctcattt tcaaaatagt
     4561 aatcgtttag ctaatagttc atttcaagat aaacgcgcat tggttacagc aggaccaact
     4621 attgaagtta tcgatccagt cagatttgta tccaatcgtt cttctggaaa aatgggttat
     4681 gcaatagctg aagcattgcg aaatcgagga gctatcgtga cgttagttgc tggtcctaca
     4741 acactagagg atccaaaaga tattgaagtt attcatgttc aaagtgctga agaaatgttt
     4801 gaacaagtga caagccgatt tgacgaacaa gatattgttg taaaagcagc agccgtatct
     4861 gactatacac cggttgatgt attagaacat aagatgaaaa agcaggatgg tgatttgtca
     4921 gtatctttta aacgtactaa agacattctt aaatatttag gtgaacataa aacatcacag
     4981 tatttaatag gctttgcagc agagactgaa gatattgaaa attatgcaca acaaaaatta
     5041 cgcaagaaaa atgcagatgt gattatttca aataatgttg gggatatgtc tatcggattt
     5101 agttctgatg ataatgaatt gacaatgcat tttaaaaata atgaaaaggt aaatatcaag
     5161 aaaggaaaaa aagtagtatt agctgcacaa attttagatg aactagaaac taggtggcaa
     5221 taatgatagc gaaagtcata gtcgatgtcg cgtcgaagag cgttgactat aaatttgatt
     5281 atataattcc cgaacaactc gaatctgtca tccaacctgg tgtgcgtgta attgtacctt
     5341 ttggaccaag aacgattcaa ggttatgtaa tggaagtaac agcagaacct gatgcacaac
     5401 ttgacgtttc gaagttaaaa aaaatcatag aagtgaaaga tatacaacca gaattaacat
     5461 cagaattaat agctttaagt gagtggatgg gttcaactca tgtcattaaa cgtatttcta
     5521 tgctagaagt gatgcttccg agtgctatta aagcgaagta taaaaaagca tttaagatga
     5581 aagatgacat agagctacct tcagctttat tacaaaaatt tgataagcat ggttactatt
     5641 attataaaga tgcgcaaaaa aataatgata ttcaattgct tatgaagttg ttaaaagatg
     5701 atatcgttga agaaaaaacg attctcacac aaaatataac taaaaaaacc aagcgtgctg
     5761 ttcgtgtcat tgaagggtat catcctgatg aagtattagc taagttggag aaagttatta
     5821 aacaatacga tttgtatgct tacttgtctg aagaacaaca taaaacaata tttttaactg
     5881 atattgagga tatgggcttt tcaaaatcca gtttagatgg acttatcaaa aaaggttatg
     5941 ttgaaaaata tgacgcggtt gttgaaagag acccatttaa agatcgtgtt ttcgaacaag
     6001 aatcaaaaca gcaattaaca gaagaccaat ataaagcata tgaagcgatt aaagctaaaa
     6061 ttgtaagcca agagcaagaa acatttttac ttcatggtgt gacgggatca ggtaaaacag
     6121 aagtatattt acaaacgata gaagatgttt taagccaagg aaaacaggcg atgatgttag
     6181 ttcctgaaat cgctctaaca ccgcaaatgg ttttacgctt caaacgtcga tttggtgatg
     6241 acgttgctgt attacattct ggcttatcta atggggaacg ttatgatgag tggcaaaaaa
     6301 ttagggatgg tcgtgcgaga gtaagtgttg gtgcaaggtc aagtgtgttc gcacctttca
     6361 aaaatttagg gttaatcatc attgatgaag aacatgaatc tacatataaa caagaagatt
     6421 atccgagata tcacgctaga gaaattgccc aatggcgaag tgaatatcat cactgtccag
     6481 tcattttagg aagtgcaaca ccatgtcttg aaagttatgc acgagctgaa aaaggcgttt
     6541 atcatttgct atcattacca aacagagtga accaacaagc tttacctgaa attgatatag
     6601 tagacatgcg tgaagaattg agtgaaggta atcggtcaat gttttcaaaa gatttacgtg
     6661 aagccataca attaagatta gatcgacagg aacaagttgt tttattttta aatcgacgtg
     6721 gttatgcatc gtttatgtta tgtcgggatt gtggatatgt accgcaatgt ccaaactgtg
     6781 atatttcatt aacgtatcat aaaacgacag acttattaaa atgtcactat tgtggttacc
     6841 aagagacgcc accgaatcaa tgtccaaatt gtgagagtga acacattcga caagtaggta
     6901 ctggtactca gaaagttgaa gaactattgc aacaagaatt tgaagatgcg cgcataatta
     6961 ggatggatgt agatacaacc tcaaagaaag gtgcacatga aaagttattg actgaattcg
     7021 aaaaaggtaa cggtgacatt ttactaggta ctcagatgat tgcgaaagga ttagattatc
     7081 caaatattac tttagttggt gtgctgaatg cagatacaat gttaaattta cctgattttc
     7141 gggcgagcga acgtacttat caactattaa cgcaagtggc tggtagagct ggtcgtcatg
     7201 aaaaggcagg tcaagtcatc attcaaacgt ataatccaga tcattattca atattggatg
     7261 ttcaaaaaaa tgattattta acattttatc gtcaggaaat ggaatatcgt aaattaggaa
     7321 agtatccacc gtattattat ttgattaatt tcacaatctc acataaagaa atgaagaagg
     7381 ttatggaagc atcgcagcat gttcataaaa ttttattaca gcatttaaca gaaaaagcgc
     7441 ttgtactagg tccatctccg gcagcacttg cgagaatcaa caatgaattt agattccaaa
     7501 ttttagtgaa atataaaagt gaacctggat tattacaagc cattcagttt ttagatgact
     7561 attaccatga aaaatttata aaagaaaaat tagcattgaa gattgatatt gatccacaga
     7621 tgatgatgta attttttgac taaaacactt taatatcaat gtttcctaag agtatgaaag
     7681 gtcttgtttt tactttaagt acagaaaaag ggcagaatat acaactgtta accatttaaa
     7741 ttagcagttt atattctgtc tttttatatg gctttataac ttacgtgatt ttgatttgat
     7801 taggaattta ttagtatttt catttacgaa ttctgatttt aatgtaggtg ccatacttac
     7861 tgatgaataa aagtactaat gattaaacaa actattttta
//
LOCUS       DBNENW010000115         7769 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNENW010000115 DBNENW010000000
VERSION     DBNENW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.116"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..131)
                     /gene="rpmG"
                     /locus_tag="AC1LW8_002040"
     CDS             complement(<1..131)
                     /gene="rpmG"
                     /locus_tag="AC1LW8_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP4773847.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQT"
     gene            complement(244..2319)
                     /locus_tag="AC1LW8_002041"
     CDS             complement(244..2319)
                     /locus_tag="AC1LW8_002041"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIP4773848.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIRKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            complement(2440..3039)
                     /locus_tag="AC1LW8_002042"
     CDS             complement(2440..3039)
                     /locus_tag="AC1LW8_002042"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIP4773849.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(3315..3725)
                     /locus_tag="AC1LW8_002043"
     CDS             complement(3315..3725)
                     /locus_tag="AC1LW8_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIP4773850.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(3712..4575)
                     /locus_tag="AC1LW8_002044"
     CDS             complement(3712..4575)
                     /locus_tag="AC1LW8_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP4773851.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(4617..5402)
                     /locus_tag="AC1LW8_002045"
     CDS             complement(4617..5402)
                     /locus_tag="AC1LW8_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP4773852.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(5528..6418)
                     /locus_tag="AC1LW8_002046"
     CDS             complement(5528..6418)
                     /locus_tag="AC1LW8_002046"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIP4773853.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(6428..>7769)
                     /locus_tag="AC1LW8_002047"
     CDS             complement(6428..>7769)
                     /locus_tag="AC1LW8_002047"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP4773854.1"
                     /translation="KHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNLIG
                     QSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGVSV
                     KVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMIDL
                     GLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNIEF
                     FLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGGLT
                     PRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRTGR
                     GNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDDHL
                     TNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
BASE COUNT         2393 a         1442 c         1048 g         2886 t
ORIGIN      
        1 aaagtttgtt tgttttcacg tgaacagaat ttcttcattt caacacgttc tggattattt
       61 cttttgttct tagttgtaat gtagtttctg tcaccacatt ccgtacaagc taaagttacg
      121 tttacgcgca tggttattcc tccttatcat ttctaaatac gactttatta ttataccaac
      181 atctataata tttgcaaagt taaaatttta caaccatgcg ctacacaatc gtcaggttaa
      241 attttatttg tctttgtctt tatttttatc atctttacct aactgcttaa agtagtagtt
      301 aattacatct ctacctaagt ctccacctgt taaccatggt ggtggtacag gctgatttgt
      361 atatacaatt gaaaacgcta attttggatc atcaattggc gcgtatccta tataagtaga
      421 gttaactctt ggctctccgt tttggaacac ttcagcggta cccgttttac cagcagtagg
      481 tactactgta tctttaaaac taacatatcc agtaccatct ttatcattaa atgccatttt
      541 gaatccttct tgaatttgtt tgatttcctt ttcagtatta ttaaccttgt tcaagacagt
      601 gccattaatt ttcttcttga gtggaccaac ctcatcttta ttagttgatt catgaatcgt
      661 taatccaatg tgtggctgta ttctataacc atcattcgct atagttgaaa catattgtga
      721 taattgtaat ggtgtatagg tatcatattg accaattgat aaatctagat aattacctgg
      781 attatttgtt aatggttcga tttgacctct tgtttcattt ggtaaatcta tccctgtttt
      841 cacacctaag cctacttgat ttaatcctct tcttagcttt tgggcaggtg aacttatgtc
      901 tgaaggtaaa gccataccag aataataagg gtctcccgct aattttaatg ctgttttaaa
      961 catatatacg tttgatgaat gcatcaaagc ttgcttatca ttaatagata catgcccgtt
     1021 tttattgaag tatgatcgtt ttgtcaaacc accttggaaa tgtaatggtt catcgaccat
     1081 tgtttctcca actttgatag ctttattctg ataaccagct aataatgttc caccttttac
     1141 agaagatcca accgcaaatt gagaagtaaa cgtaccaatg tcataatcag tcattttacc
     1201 actcttatta atctgctttc cggcaagcgc aagaatgtct ccatttttag gattttgtac
     1261 aaccatcatt gcattatcca tatctttggc accttgactg cgaagcttct taatttgttt
     1321 atctaataat gcttctactt ctttttgaag atctatatcg atcgttaatt tcaaatcttg
     1381 accgcgagcg ccaggattta acacttctga agatgtaact ttaccagatt tgtccgttgt
     1441 gtatttcatt tctttcttct taccacgcaa tacatcttca tattgatatt ctaggtaaga
     1501 ttttccaaca cgatcattgc gtgaatatcc tttggataag taatgttctg tcaattcttt
     1561 tggaatacct tcagcaggtg tcgatacatc tccgaatata cctcttaaag tatcgccata
     1621 tggatatttt ctatcccaat ccatagacgt gttaacacct ggtaatttgg aaagttgctg
     1681 agaaactgct gcatactctt tttcactgac atcttcattt tttatcattt gtggatctaa
     1741 aactgttcct gcattcatct ctcgaaaaat agctaaaact tgtaaatctt tagaagacaa
     1801 ttcatctaat tgtgattttc tgattttcga taacagttgt ttatcatatt gatcttgttt
     1861 aatacttcca tctgctaaca tagcttgttc ttttgtcatc attgcttttg cttttttagg
     1921 atgcaactga atccagaaat ctttcttatc acgttctgta attttcttag tatccatctt
     1981 gattagcttt gataactttt cagccgtatc caacatttcc gattgtgttg tttttcgacc
     2041 cctagtatat gtaatagcca ttttagaagc attatcaact aaaactttcc catttctgtc
     2101 taaaatacga cctcttggca cagactcatt cactgtaatg ttttcatcat tttttataat
     2161 ttgtttataa tgtgagcctt gtgcgatttg taaataacct aaacgtagta ctagtactgc
     2221 aaaaataaat acaatcacac caaatataaa gttaattctc ttgttaattg tattttgaac
     2281 gatttcatca tttgattttt cttttagtct ttttaacaaa actacctacc tctattcaaa
     2341 gtctttcact ttaaatcata tatgaattta gaaattattt ctatcttttt gacaaaaaaa
     2401 taacggtctc atttaagaga ccgaacaagt aatcatactt tattttgttg cattatataa
     2461 ttcgtcaact ttttcccagt taactacatt ccaaaatgcg ccaatgtagt cagggcgttt
     2521 gttttgatat tttaggtaat aagcgtgttc ccatacgtct aaacctaaaa taggtgtttt
     2581 accctcagtt aatggattat cttggtttgg tgtagtcaca atttctaact ggccattgtt
     2641 tacgactaac caagcccaac ctgaaccaaa gcgtgcagct gctttgtcag caaattcttt
     2701 tttaaattct tctaaagaac cccattgttc tttaattttt tctactacag tacctttttc
     2761 ttctgagttt ggtgaaagta actcccagaa taatgaatgg tttaaatgtc caccgccatt
     2821 attacgtaca gcagtttgga tgttagctgg tacactgtct aaattagcaa caatttcttc
     2881 aatagattta gattctaaat ctgtaccttc tactgcagca tttaatttcg taacataagt
     2941 gttatgatgt ctgtcatgat gaatttccat agtttctttg tcaaaatgtg gttctaatgc
     3001 atcaaatgcg tatggtaatt ttggtaattc aaaagccata aataatcatc ctcctaaaat
     3061 gtctgtaagt aaataataac aagcagtgac tggttcaaca aagaatttgc ttaaattcta
     3121 ctacttatta ttttctctac tcatttaata taactcaaat caaaaataat taaacatttt
     3181 gtatataaaa agttaacaga tttgccataa aatcatacga acggagtatg aaatgaacct
     3241 ttatcttcta taatttaaaa aatgagattt atgcatacat ctgaccaaat gtgcataaat
     3301 ctcatttctt tatattaatc ttggcaagac tcacatacac cataaacttc aagtttgtgt
     3361 ttgtgaatat taacaccagg tagtgataac tttatctgat ctattggaca ataatctatt
     3421 acctttgtat ctccacactt ttcacagata aaatgatgat gatgatggtt tgtacaagcg
     3481 attctaaact tcatttcacc atcaagttct gtattttcaa taatccctaa atctttaaat
     3541 aagtgcaggt ttctatatat tgtgtcgaat gaaattccag gataattttc atccataact
     3601 tgctgtatat actttgcgtt tatatactta tcttcttcga caaaaatatc taacatatct
     3661 ttacgtttat ctgtatattt taaaccgttc tcttttaaaa ttttaatagc atcatttgta
     3721 ttcattgata ttagctccct ttttaaactt cattcgcatt ttctgataag ccattgtaat
     3781 cataagtaaa ataacaagta gaactacaat tacaccaccc ggagaaatgt ccatatagaa
     3841 agctaggact aagcctaata ttactgataa ttcacctaaa aatacactta gtaatatcaa
     3901 ttgcttaaaa ctttttgtta ttcgcatact tattgcaatt ggtaacgtga ttaacgcact
     3961 tactaacagt atccctacaa cacgcattga ggcagaaata accatcgcta caataacaat
     4021 aaataaaaat tgaatccatt taggaatgcc aatgacttta ctatattcct catcaaatga
     4081 caatataaat aattctttat aaaacaatgt aataaacaga acaactatga tggcaatgac
     4141 aataatcgtt gttaaatcac ttatattcac tgcgcttatt gagccaaata gcaatccaac
     4201 aatttcttga ttgaacccat cagctaatga aatgaagatt gcactcaagg cgataccagc
     4261 actcattata attggaatag caatttcttg gtaagcagtg tatgacgttc ttaatttttc
     4321 aattagaagc gcacctacta ttgcgaataa gattccaaac cacattggat taataaatac
     4381 tagtgttggc ataatagtaa gtaaaaacat accgaaagat ataccaccta aagttacatg
     4441 acttagagca tcagctataa gtgatagtcg tctaacaacg ataaaagcac cgattagagg
     4501 cgcaataaaa cctatcaaga taccactaat taaagagtac ctcataaaat caaaattcaa
     4561 taatgcatct atcaattgtg acacgccttt ccattttaaa taaactcaaa tctttattaa
     4621 ttacaacatt ctcgattatg ctgatgatcg acaaaacgta caggatgtcc ataaattttt
     4681 gaaatttcaa cttcatcaag tgatttaaac tcatcagttg taccatggaa atgcaaatgc
     4741 ttatttaaac atgctacttc agtagcagta tctgctacaa caccgatatc atgagtaact
     4801 aagataatgg tgataccttc ttgttttaat tgatctaaag tattataaaa ttcacttaca
     4861 tgttttgcat caataccatt cgttggttca tcaagtacta atactgcagg ttctgaaatc
     4921 aatgctcgag caatcattac acgttgttgt tgaccacctg ataattctgc tatattttta
     4981 tgaattaaat cacttatatt cagtctttct agtactttaa tcactttttc attatctttg
     5041 ctattaaatg tttggaaaag acgttttgtc tttgttaatc cgcttaaaac aacttcttta
     5101 acacttgctg ggaaacctga attaaaggca tttgcttttt gtgatacata gcttaattta
     5161 attgatgttt tcttattttt aaaatcaata ccttcaacaa aaatctcacc actttgtaaa
     5221 ggtaataacc ctagaatcaa cttcaataat gttgatttac cagcaccatt tggtccaaca
     5281 attgctaaaa attcaccttt atttatttta atgtttatat tttctaacac ttttttatga
     5341 tcatagtagt aattgacatt tttcaattca aagactggtg tcatcgtatt ctcacctcgc
     5401 attcaactat acaactccta gtaacatatg taaacagtaa tgtttacgac tcaaaattag
     5461 acaaaataaa gagatatgcc cccttcaagt tttatttatc gcatttcttg aagagagcat
     5521 tatcatttta ttgttgcata accttatttt ttaattctgg gtcaaattgc tgttgtttta
     5581 acatttcaat ttcaagttta tatggcggtt ttttattttt cttatcttca ccaacataag
     5641 gtgtttctaa gattttcgga atatctttaa aactatcatg atgcacaatg taatttaatg
     5701 catcaaaacc aatgtaaccg aagccaatat tttcatgtcg gtctttttga gcgccacggt
     5761 catttttaga atcattgaca tgaacaactt tgattctgtc gactccaata attttatcaa
     5821 attcatttaa tacgccatca aagtcctctt tcacattata tccagcatca tgcgtatgac
     5881 atgtatcaaa acatactgat aaacgttcgt tattatgaac tccatcaata atacgtgcta
     5941 actcttcaaa cgagcgacca atctctgtac ctttacctgc catcgtttca agcgcaatac
     6001 gtacattatt gtcattcgtt aaaacttcat ttaatccttc aataatctta ttaattccgg
     6061 catcaacacc agctccaaca tgcgcacctg gatgtaatac aatatcttta gcgcctatag
     6121 cttgcgttct ttcaatttct tgttgcaaga aatctacacc aagattaaac gtttctggtt
     6181 tggttgtatt tgcaatatta atgatgtatg gtgcatgaac aacaatatta gataagccat
     6241 atttttccat cacttcatga cctttagtta tatttaaatc ttcaatactt ttacggcgcg
     6301 tgttttgagg tgcaccagta taaatcataa atgttgtttc accatattca tacgcttcta
     6361 tagcagaacc ttctaacatc tttttaccac tcattgaaac atgtgatcct aataacataa
     6421 aacacaccta accttttttg ttttgcttac gtttttgtct attttgctgc ttactaaatt
     6481 gtttacgctc ttgacgtttc attttttcaa cttcttgttt aaatttcttc ttataacctg
     6541 gtttaacttt gtttttaatt ttacttcgaa ctttgttctt cacttgatta gttaaatggt
     6601 catctttgcg cattcttgct tgacgctgat tgtgcgcttt aacttctttt aactcaccat
     6661 ctttaatatc aacagtattg aatacaaaac cgcgatcttc tattaatgaa atattgtgtt
     6721 cttcatcagg actataaagc gtaattgcta cacctttata attcccacga ccagttcgtc
     6781 caactctatg cgtaaagaag tcaatatcat ttggcacatc aaaattgatg acatgactaa
     6841 caccttcaat atcaatacca cgagatgcta aatcgctggc aataacgtat tggaattcta
     6901 aattacgtat acgtttcatt tgttgtttac gttcacgtgg cgttaaacca ccatgaatca
     6961 taccaacttt aataccagct tcatttagtg aacgtgctaa atcatttgca ttatctctac
     7021 tattacagaa aataatacat aagtatggat ttagtatatc aattaaattt aaagtttttt
     7081 caactttagc tgcaccttta gtaggtatta aaaagaattc gatgttcttt ttattttgtt
     7141 ttttactgtc gacagctaca tattctggat gacttaaata tttatttaaa aatggttgta
     7201 actgttgtgg aattgtagca ctaaacaccg caatatttgc attatcttcc aatcttgcag
     7261 caatgtaatc tacatcttca attaatccta agtcaatcat aagatccgct tcatcaataa
     7321 ctaaatatga tgctaagtgc acatgtaaat gtcccgtttt agctaagtca ttaattctag
     7381 taggggtgcc tataatcaat tgtggttgtg cattacaacg ttgtctatct ttctctatat
     7441 ctgtaccacc aataaaaact ttaactgaaa caccagcttt aaattggctt aaatggttcg
     7501 ctgcatcgta tagttgttgt gcaagttctc ttgttggtgc aactacgatt gcttgtggtt
     7561 cttttatttc actatcaatt aactgcatta atggtaataa aaatgcatga gatttccctg
     7621 tacccgtttg agattgacca attaaatttg ttctctttag tattcttgga ataattcgat
     7681 tctgaatttc agttggtttt tcaaaattaa ggtctttcac agcgtcaatt aaactagatt
     7741 ctagattaaa ttgttcgaat ggatgtttt
//
LOCUS       DBNENW010000116         7725 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNENW010000116 DBNENW010000000
VERSION     DBNENW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.117"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(68..394)
                     /locus_tag="AC1LW8_002048"
     CDS             complement(68..394)
                     /locus_tag="AC1LW8_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP4773855.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYKVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(458..1201)
                     /locus_tag="AC1LW8_002049"
     CDS             complement(458..1201)
                     /locus_tag="AC1LW8_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HIP4773856.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1228..3183)
                     /locus_tag="AC1LW8_002050"
     CDS             complement(1228..3183)
                     /locus_tag="AC1LW8_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773857.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMVKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITMSDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3793..4602
                     /locus_tag="AC1LW8_002051"
     CDS             3793..4602
                     /locus_tag="AC1LW8_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4773858.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4619..4861)
                     /locus_tag="AC1LW8_002052"
     CDS             complement(4619..4861)
                     /locus_tag="AC1LW8_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773859.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5035..6435)
                     /gene="nhaC"
                     /locus_tag="AC1LW8_002053"
     CDS             complement(5035..6435)
                     /gene="nhaC"
                     /locus_tag="AC1LW8_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIP4773860.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6530..7612)
                     /locus_tag="AC1LW8_002054"
     CDS             complement(6530..7612)
                     /locus_tag="AC1LW8_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HIP4773861.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         2644 a         1414 c          982 g         2685 t
ORIGIN      
        1 tgccatttat ctttaaatgc aatgagttat cattcaaact cttcttatca acctacatac
       61 attgacatta tactatcata tgttgtttta catattcaac aaacatttca acgttatcac
      121 ttttaatatc atgcattggg acattaccaa tattaaattt gaacattata gtttgaccat
      181 cataatctat cttctctatt tcattataac taatacttct ataataaaat tggccattca
      241 tatctacatt cataattaat ctttcatttg ttgctataaa tgccccttca aattcattct
      301 caccttgaac tttatactca attattccta gcactgacgg accctttttt tcagttggaa
      361 ataaatcatt tggattcaca ttatctaaaa tcataatatc cctcccactt aaaactataa
      421 actattcttc ataggtatgt gaaatacaag tgattaacta tttattatta agcttaactt
      481 gtattccctt ttcaagataa ttataacatg cttgaataag catagtgact ttagaaaact
      541 tttctcgctt gattgttaca ataacacgcc tttcatctaa ttcacatctc tcttttttaa
      601 ctaatccctt caaaattaaa cactttaaaa tcgggctaac cgcaaatatc gggtgatgga
      661 attcaccctg taattcttta accgtcaatt gacctttatg cagatttaat attcctaaaa
      721 cgtataactc ttctaatgtt aattgacaca tatttaaata acactttaga tttcgaaata
      781 attcttgtga cgacaataaa tacgatacat agtcacaact aaaatcaaat gtatattgac
      841 caaaataacg ttcaatatag tcactaagct cattattcat atattctatt ttatctatat
      901 gcattttatt aatcgaaata gtcaatttac gttgatccct ttgatctctt cctttcaata
      961 gccattgatg attgtttaaa taacttaaca tttgaagaag ggccatctta gattggattt
     1021 ctttcatttt taataaacta tctattgaag cgctattgga atcagaatta tcatatttaa
     1081 ctaatagatt taatagtttc acttgagtca aagtgagatg aaatgaatat ttcaacatat
     1141 cattaatgac tttcaacatt agtatgtgtg cagttaaacg cttaacttta aatttagaca
     1201 tctttaaaaa cctctcttaa accatgccta tatctcaaga tgatatttca aatgaacaat
     1261 actattgctt gagaccatta atgaatgatc ataaatattt ctttctataa aattagcttt
     1321 ccaataactg tgttgttgca taatatcatt cacaagtaca ccattttcgg aagtatgatt
     1381 atctttatct atacttaaaa caatttgttt agttttagca tggctaaatt gttgaagacc
     1441 cttacacgat aaacgtatag cgtctgaatt ctcatttaac aatgcggctg gcaaacaaat
     1501 gacacattgt acttcatgtt tgaacttcgt tacaatcatc gtgtcatttt gataaataac
     1561 aatccctcgt aattgattaa gtatatttat gataaaaatg aatattgata atctagttaa
     1621 atttatttct ttgctcgctt cacttataat tgacatgtgc ataataagtc ctcgaaattt
     1681 tgtataattt gtcaaaagtt cagtaacctt attaaaatca gacattgtga ttttttcttt
     1741 taagtcatta ttctcattaa tcaaatctac atcacatata attttagaac atgcaataga
     1801 cacctgttct atttgtctta gcggtaagat aaaatagtct attagatgat caatttcttt
     1861 atcatagcgc gtattcaact cttttggttc cagatgatat attaattcaa atactcctat
     1921 ataattatct attaaacgtt tcagtttttg aattgcagta gtatttaagt cgttaattaa
     1981 aaatgcaatc ttacatctat tactctcaaa agtcatatta aaaattgtag ctactagctt
     2041 ttctatcttg cctctttcag tactcgttaa caattcctta tattcatgtt taatacgaaa
     2101 tactacattt acattgtaat tcactatctt ttgaagtaat aaagtgattt gttcaatcga
     2161 tagctcattg cttgtgtcga ttgtaacaat tatatgcaag ttttcaggat taacacctaa
     2221 tctttgaatg atttgtttaa tagtataata atccatccaa taaaaattac ttcctttaat
     2281 ataaatgttt ttaggttgat acatttgact cctttttgta ggctcaaaag gtatatcaat
     2341 ctcgcgcata cttgaagaac tttgattagt atcatcaaat aattcaatta tatttttatc
     2401 aatttcaact aattgggaat ggttaattga atgagatgtt ggactatatc tttttctaat
     2461 taatttagga gtgttaccat acgtttcttt aaagaggtgt ataaaacgag aataatgatt
     2521 aaaaccatga ctacttgcga tttcttcaat aggcttctta gaagttaaaa tatcaattaa
     2581 gcaatgctcc agtctaatat gatttaaata ttgaataaaa ttactataag gtgtcgcttt
     2641 aaccatgtca cttaaagctt tgtttgtaat actaacttga ttaatgacat ctttcctatt
     2701 tatcttttta tggtggttgt ttgttaaata atcgtgcact tcttcggcta ctaaatgacg
     2761 actaccatcg tatgtattta atgacattaa ttcaacacac atgtaattaa taatcttatc
     2821 attagcatta taagactgtt gtttaatttt gctgtagatt aaatacttaa tcaagattct
     2881 aaaagtgcta gcaacctcat ttgttaatat cttgccacgc atcatatagt ttgcattcat
     2941 aaacttcaaa aatgtctttg cacttacttc aacaacacaa catacactat catcatgacc
     3001 atcaatttga tacaaatcat tcaacataat aacggtgaca tcatttcttt ttacatcata
     3061 ttgttgtaga ttgattgaaa ctacacctgt accttgtagc caatatataa tttttaagtt
     3121 cgcttccttc acactactca ttttcatact tttataaata tgtagtgaat aattatctgt
     3181 catcttacat cctcacatat acaaatatat tgatttactc ctgtttgtct ttctcaaatc
     3241 aattgtcaac atctaatatt cactaccaat tatatcgcca catgtttttt agcagtaaca
     3301 aaacataaat cgattatatc aaataatact tttattaaaa cattttttta tgctaaatta
     3361 ggatatttta tccattaaat atacattttc atccaaatac caaagctttc ataatcatag
     3421 aaacttgatc gttcgtgtcg aatttttatg cattttctga ttaaaagtcc tatattatgc
     3481 acaaaatata catttagaat tttcagtaat attacaaatt tgtaacagac ttattttcga
     3541 aacattcagt tttcacaaag aatgccgtag ttataagttt tgcaaaatgc aaatttctat
     3601 acttttaaaa ttctattgtg tcaagatatt agcaaaaaat tacaaaacgt taacaaagca
     3661 cacaaggacg ctaatttctg ttatagtctt tcttgtcgtt taattacgag agataactta
     3721 taacattaat tttgtcacac ggacattacg aaaatatttt agatttaaaa tttttaggag
     3781 gatattttta aaatgaagaa aatcgctaca gctactatcg caactgcagg attcgctaca
     3841 atcgcaattg catcaggaaa tcaagctcat gcttctgagc aagataacta cggttataat
     3901 ccaaacgacc caacatcata tagctatact tacactattg atgcacaagg taactaccat
     3961 tacacatgga aaggtaactg gcatccaagt caattaaacc aagataatgg ctactacagc
     4021 tattactact acaatggcta caataactat aactacaaca attacaacaa tggttatagc
     4081 tacaataatt acagccgtta caacaactac tcaaataata atcaatcata taactacaat
     4141 aactataata gttacaacac aaacagctac cgtactggtg gtttaggtgc aagctacagc
     4201 acttcaagca acaatgttca agtaactaca actatggctc catcatcaaa tggccgttca
     4261 atctcaagtg gttatacttc aggacgtaac ttatacactt ctggtcaatg tacatactac
     4321 gtatttgatc gtgtaggtgg taaaatcggt tcaacttggg gcaatgcaag taactgggct
     4381 aacgcagctg caagagctgg ttacacagtg aacaatacac caaaagctgg tgcaattatg
     4441 caaacaactc aaggtgcata cggtcacgtt gcatacgttg aaagtgttaa cagcaatggt
     4501 tcagtaagag tttcagaaat gaactatggt tatggcccag gtgttgtaac ttcacgtaca
     4561 atctcagcta gccaagctgc tggttataac ttcattcact aattagtgat ggctatgttt
     4621 acgccaacaa ggtttcaaga gccttgttgg cttttttaat ttctatgaaa aaaatcgaat
     4681 tggtaaaaat ctcaaaattt cgattttcac caatccgatt cactctgtaa taaagaataa
     4741 acaacataca gccattgaca cacccaaatt taggcatgtc aaatcggcta tacattgata
     4801 aatttttagt taaagtactt acaaatatac ttgccttcaa attctcaatt aatttagtca
     4861 ttacaaatca tgacataaaa cttacattac atatatcaac atgttccgct acaaaacata
     4921 ttcagtatat gtatagagga tatttattct aagttaaaac cttttcaaac tataaccgct
     4981 acaattataa atgattaaag tgtttcaaca tattatatat attccacgct cacattaagt
     5041 taaacgtgaa ttcgatgact ttttaatacc tatccctgta aaaccataga taattgctat
     5101 aattgcgcat aaataacatg gtactgtcca tatgaaaaat tcttcaacag agacatgaag
     5161 ttgattcgta tagtaaatac ctgatgttcc ccatggtatt aaaggtaaga ccatcgtact
     5221 tgagtcttca agtgttcttg ataaaacgga acgagagact tggtattttt cgaacaaatc
     5281 tttcattaac acaccgacca taataattac aatcgaagca acacctgcag cgaatacaag
     5341 cgcaatacaa caaataacag taatacatat taaacttcct acagaatgga tgcctttaga
     5401 aatagtagtt aataagactt ctaaacatcc tgctttttct acaatacctg caaatgcata
     5461 tccgcaaaat atcgttacta atatttgggt catactcatc ataccaccct gttctaacaa
     5521 gcttttcaca ctggatgaaa tatgagactg atgtatcatt gagtcgttaa aaccactaaa
     5581 tgttgctttg aaaccatctg tcattttgaa atgatgatta aaagtcccca ctataatggc
     5641 agaaaagctt gatattagca tagctggcac tgtagccatt ttaaatagca aacaaacaat
     5701 gatcacaatt aagggaaccc agacccatat gttaatttga taaatctgtg caagctctga
     5761 taacaaagtt tgaatctgtt tatcatttga atgcccttta aattgaaatc cagcaataaa
     5821 ccatactaat aaacctatga ttgatgcagg tatcgtcgtc cacatcatcg aatgtatatg
     5881 tttaaatata ttaactttag taacaagcgc cgctaaatta gttgtatctg ataatggtga
     5941 cattttatcg ccaaacacag cccctgctat aatagcaccc gctgccatcc ctggaggaat
     6001 ccccaattga ttaccaatag aaataagtgc aatccctgca gttgatgcag agccccatgc
     6061 tgtaccagtt gctacagatg taacagcact tataaaaaag gctgatatta aaaaatagct
     6121 tggattcaat aaatctaagc cataataaat caaggctggc actgtgccag aaaacatcca
     6181 actgcctact ataattccta ccgctagtat aattaaaatt gcaggcattg cagtatttaa
     6241 acgttctgca atgccttttt ctaaatcttg ccatgttaag cctacacgtt ttgcaatcca
     6301 tgtggcatat gctgaggcaa taattaataa tacttgaatt ggaatatcaa aaaacacaaa
     6361 gcctgttaca acaacaataa ccattacaat cattgtcgaa attgattcta aaaaggttgg
     6421 ttttcttgtc atcatctatc gtccccttcg cttattgatg agaaacctct tttcttcaat
     6481 ttattgcttt tgcataatgc tatatccttt tctatcttca agccatcatt tattttaaat
     6541 atttttctaa atcaagctta tctaaaccaa tttcttcaac tgttaagcct tcctcaaaga
     6601 agtctctctc taaaatggtt gatgcaatta caattactgc atctatattt ggtgtcggta
     6661 catcaataac acgacctaag cttgaccata gtactaaacc atatgcaata tcttcagtta
     6721 aatatctgct ttctacatga tttggtcctg gaatttgtga aaatactggg cttgtattaa
     6781 acaaacgatt taatggttca tcttctttat cacgttctaa ataaccacgt tcaatacgtg
     6841 attctttagc tgttgataat tcaaaaccta atctacggcc taaattcaaa cgttctaatt
     6901 cgattgcatg aagtaatcta actgtatgtt tagtaattcc ttctttatat aaagcgaact
     6961 cgccagcata atcaatgcga ccgacattca ataatgttgg tcctggatgc acttcaggat
     7021 taccattttc aagatttgtt ttaattaagc tttcctcttt tactaaatga tcataaatac
     7081 ttgaaacttt gtcataacaa tcatttagac agcttctatc atatgttgaa aagaagatac
     7141 gacgtacatt tagagataaa tcaactgctg cattttcaaa atcgacacgc gtaccatacg
     7201 tcaacgtatt agcttccgct agttgtggtt ttgtttcaat atgtctatct tctaaaacat
     7261 tcataaaacg aattgacccc attgctgcag ccatgttgaa gaatatcaac tgattatcag
     7321 ttacatgctc tgccattaca tcagcataat actctatgta tgaagatgga ataatcactt
     7381 gaacaatttc agcatctttt aaaacatatt ccatatcatc actaatatca gtgaatttta
     7441 cgaaacgttc atcaccttca ttattaaaat caaatccgcc cttttcgatt gcgttttgaa
     7501 acttacttat agattgatta cgacaatata atttaacatc gtggcctttg ctcaccatat
     7561 ctactgctgc cgtaactgcg ccatttcctg atcctacaat tgctattttc ataaacaacc
     7621 ctctttctat ctttggttaa aattaaaaac aaattatacg taagacaata taaaaatgat
     7681 gcgaattcat tacgcatcat tataaatgtt aagtatatat aacct
//
LOCUS       DBNENW010000117         7606 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNENW010000117 DBNENW010000000
VERSION     DBNENW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.118"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            6..704
                     /locus_tag="AC1LW8_002055"
     CDS             6..704
                     /locus_tag="AC1LW8_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HIP4773862.1"
                     /translation="MKLKALAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELINVDGLYRQHLTDKGAYVWKNLKDYYIGLLGEDSKKFKSDVYGDLDAF
                     LVIEEEPVKGRQYSIGGISKTNSKEFKEREVDVKVTRKADRDTTSTKDSKFKITKEEI
                     SLKELDFKLRQKLMKEENLYDAINHRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTID
                     GTKIKEINVELEYK"
     gene            1126..1821
                     /locus_tag="AC1LW8_002056"
     CDS             1126..1821
                     /locus_tag="AC1LW8_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HIP4773863.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLSGKDKDKYKEGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNQSSETNTPLFIKKVYGGNLDASIESFLINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITFNLKDGEKQEIDLGDKLQFEHMGDVLNS
                     KDIQNIAVTINQI"
     gene            2160..2858
                     /locus_tag="AC1LW8_002057"
     CDS             2160..2858
                     /locus_tag="AC1LW8_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HIP4773864.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSYDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGAADKGRIVINMKDENKYEIDLSDKLGFERMADV
                     INSEQIKNIEVNLK"
     gene            3237..3935
                     /locus_tag="AC1LW8_002058"
     CDS             3237..3935
                     /locus_tag="AC1LW8_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HIP4773865.1"
                     /translation="MKFTALAKATLALGILTTGVFTTESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKIDPKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            4294..4977
                     /locus_tag="AC1LW8_002059"
     CDS             4294..4977
                     /locus_tag="AC1LW8_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HIP4773866.1"
                     /translation="MKLTAIAKAALALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            5239..6795
                     /locus_tag="AC1LW8_002060"
     CDS             5239..6795
                     /locus_tag="AC1LW8_002060"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIP4773867.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQEDYVLFIDASNDFEKGKNQNHLTDAQVERIINTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEVEQEINAY
                     LKELGVLKDE"
     gene            6788..>7606
                     /locus_tag="AC1LW8_002061"
     CDS             6788..>7606
                     /locus_tag="AC1LW8_002061"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP4773868.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGDLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVEIHANLNQHVCIIRLKKEHYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGEFISKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRF
                     KDEGGKDYPDWKSKSIQEIFENKGGTALETEFNFDGNYKVISIGSYSINSTYNDQ"
BASE COUNT         3032 a          972 c         1388 g         2214 t
ORIGIN      
        1 gtactatgaa attaaaagcg ttagctaaag caacattagt attgggattg ttagctactg
       61 gtgtaataac aacagaaagt caaacagtaa aagcggcaga atcaactcaa ggtcaacaca
      121 attataaatc attaaaatac tactatagca agccaagtat agagttaata aatgtagatg
      181 gtctgtatag acaacattta actgataaag gtgcatatgt atggaaaaat cttaaagatt
      241 attatattgg gctactaggt gaagatagta agaaattcaa atcagatgta tacggggacc
      301 tagatgcatt tttagtcata gaagaagaac ctgttaaagg aagacaatat tcaattggcg
      361 gtataagtaa gacaaatagt aaagaattta aagaaagaga agtcgatgtt aaagtaacaa
      421 gaaaagcaga cagagatact acatcaacta aagatagtaa atttaaaatt acaaaagaag
      481 aaatctcgtt aaaagagtta gattttaaat taagacaaaa attgatgaaa gaagagaatt
      541 tatacgatgc aattaaccat agaaaaggta aaattgtagt taaaatggaa gatgataagt
      601 tttatacttt cgaacttaca aaaaaattac aaccgcatcg catgggtgac acgatagatg
      661 gtaccaaaat caaagaaatt aatgttgagc tagaatataa ataatctttg gacaagcaga
      721 ctagtaattg tagggaagtt aagcgataac atattgctta gcttcttttt tgttttgtta
      781 tgatgaaaaa aggagcgggt ttatgatcaa gtttttggaa aaacggttga tacttatagt
      841 cgcgcgttgt ccttttcgtg acatgaaaca atgtggaaaa cataattaaa ttgagggaaa
      901 gtgtgaatag ttaaaaaatt agtattgtgt tataaaaaat aattaatact gttaggattt
      961 cattaactaa cttaacgttg gttcaaaaat agttaaaaag aggttaattc atagcgcagt
     1021 atctcactta tataatgata gtagattgtt cgtattacgt aattgaatta atcatataaa
     1081 aatatattaa gacaaaattt ataaatagat tgggagaata gtactgtgaa attaaaaacg
     1141 ttagctaaag caacattggc attaggctta ttaactactg gtgtgattac atcagaaggc
     1201 caagcagtgc aagcaaaaga aaagcaagag agagtacaac atttatatga tattaaagac
     1261 ttacatcgat actactcatc agaaagtttt gaattcagta atattagtgg taaggttgaa
     1321 aattataacg gttctaacgt tgtacgcttt aaccaagaaa atcaaaatca ccaattattc
     1381 ttatcaggaa aagataaaga taaatataaa gaaggccttg aaggccagaa tgtctttgtg
     1441 gtaaaagaat taattgatcc aaacggtaga ctatctactg ttggtggtgt aacgaagaaa
     1501 aataaccaat cttctgaaac taatacacct ttatttataa aaaaagtgta tggcggaaat
     1561 ttagatgcat caattgaatc atttttaatt aataaagaag aagtttcact gaaagaactt
     1621 gatttcaaaa ttagacaaca tttagttaaa aattatggtt tatataaagg tacgactaaa
     1681 tacggtaaga tcactttcaa tttgaaagat ggagaaaagc aagaaattga tttaggtgat
     1741 aaattgcaat tcgagcacat gggcgatgtg ttgaatagta aggatattca aaatatagca
     1801 gtgactatta atcaaattta aagtaagtaa tcaatgactc taaagtaata aatttgaagc
     1861 agcttagcga tgaaatgttg aatagatacg tacaccttac ataaaggagc gtatttaaaa
     1921 caaccttgtc gttaggcttt ttttacgttt tataacgcag ggtatgagcg tactaaaaat
     1981 tcacattact tctgaaagtg atgtccattg aatattaatt agttcttcat taaccatgat
     2041 ttaattttaa ttaaacgagt gttaatgtca gtctgtctca atgcccttta taataaatgt
     2101 gtattattca aattacgtaa taaaagcaat ccaatatatt aagattggag catatgaata
     2161 tgaaatttac agcgatagct aaagcgatat ttgtattagg aatattaaca acaagtgtaa
     2221 tgataacaga aaatcaatcg gttaatgcaa aaggaaagta tgaaaaaatg aaccgtttat
     2281 atgatacaaa caagttacat caatactatt caggacctag ttatgagtta acaaatgtta
     2341 gtggccaaag tcaaggttat tatgactcta acgttttgct ttttaaccaa caaaatcaaa
     2401 agttccaagt gtttttattg ggaaaagatg aaaataaata caaagaaaaa acacatggtt
     2461 tagatgtctt tgcggtaccg gaattagtag atttagatgg aagaatattt agtgttagtg
     2521 gtgtaacaaa gaaaaatgta aaatcaatat ttgagtctct aagaacgccg aacttactag
     2581 ttaaaaaaat agacgataaa gacggttttt cgtatgatga atttttcttt attcaaaagg
     2641 aagaagtatc attgaaggaa cttgatttca aaataagaaa actgttaatt aaaaaataca
     2701 aattgtatga aggggcagct gataaaggta gaattgttat taatatgaaa gatgaaaata
     2761 agtatgaaat tgatttaagt gataaattag gtttcgagcg tatggcagat gtcattaata
     2821 gtgaacaaat taaaaacatc gaagtgaatt tgaaataatc aatgatatat atagaatgaa
     2881 agcttaagaa gcggtttaat aatcccatgt ttaatgattt tgatacgtgt tttaataata
     2941 aaaacatatc gaacattgac tacgttatta agctgctttt ttgtacactt tgtatcgaat
     3001 aacttaagat ctaaaactaa tcggaaagaa caatgattcc cctaaaaaaa tttatgttgc
     3061 tattaaaaat cagttaatac gaatgttaac atacgtttga ttttcattaa taatgattca
     3121 agtttattta aatgagcgtt aatgtcagtc tgttttgatg caccttataa taaagacaga
     3181 tagttcaaat tacgtaataa taacaatcca atatatcaag attggagcaa ataaatatga
     3241 aatttacagc attagcaaaa gcaacattag cattaggaat attaactaca ggtgtgttta
     3301 caacagaaag taaagctgtt cacgcgaaag tagaacttga tgagacacaa cgcaaatatt
     3361 atatcaatat gctacatcaa tactattctg aagaaagttt tgaaccaaca aatattagtg
     3421 ttaaaagcga agattactat ggctctaacg ttttaaactt taaacaacga aataaagctt
     3481 ttaaagtatt tttacttggt gacgataaaa ataaatataa agaaaaaaca catggccttg
     3541 atgtctttgc agtacctgaa ttaatagata taaaaggtgg catatatagc gttggcggta
     3601 taacaaagaa aaatgtgaga tcagtgtttg gatttgtaag taatccaagt ctacaagtta
     3661 aaaaaatcga tcctaaacat ggcttttcga taaatgagtt gttctttatt caaaaggaag
     3721 aagtatcgtt gaaggaactg gattttaaaa taagaaaaat gttagtcgaa aaatatagat
     3781 tgtataaagg cgcgtcagat aaaggtagaa tcgttattaa tatgaaagac gaaaagaaat
     3841 atgtaattga tttaagtgaa aaattaagtt ttgatcgtat gtttgatgta atggatagta
     3901 agcaaattaa aaatattgaa gtgaatttga attaattaag tataataact taagaagcga
     3961 cttaacgaca aaatgtgaat tgacatgcat gtccttaaat aaggaactgt gttaaataca
     4021 ttactgttgt taagttgttt tttgcgtttc aaagagcaga acagagtaac atcatcagtt
     4081 gtagtaaacg ataatctagt aaaacaacta aatgaaataa tgaaattcat ttaacctgaa
     4141 cattaaaata tatttgtttt tcattaagaa taattcaagt atatttaaat cgaggttaat
     4201 tatcgtatga aacgatgcac gttataataa aaatgtatga ttcaaattac gtaatgaaaa
     4261 caatccaata tattaagatt ggagcaaaac aatatgaaat taacagcgat agctaaagct
     4321 gcattagctt taggaatttt aacaacagga actttaacaa cagaagttca ttcaggtcat
     4381 gcaaaacaaa atcaaaagtc agtaaataaa catgacaagg aagcattata ccgatactac
     4441 actggaaaga ctatggaaat gaaaaatatt agtgctttga aacatggtaa aaataacttg
     4501 cgttttaagt ttagaggtat taagattcaa gttttactgc ctggaaatga taaaagtaaa
     4561 tttcaacagc gtagttatga ggggttagat gtgttttttg ttcaagaaaa aagagataag
     4621 cacgatatat tttatactgt tggtggtgta atacagaata ataaaacatc tggagttgtc
     4681 agtgcaccaa tattaaatat ttcaaaagaa aagggtgaag atgcttttgt gaaaggttac
     4741 ccttattaca ttaaaaaaga aaaaataaca ctaaaagagc tggattataa gttgagaaag
     4801 catctaatcg aaaaatatgg actttataaa acaatctcaa aagatggtag ggtcaaaatt
     4861 agcttgaaag atggcagttt ttataacctt gatttaagat ctaaattaaa attcaaatat
     4921 atgggggaag tcatagaaag caaacaaatt aaagatattg aagttaactt aaagtaaata
     4981 attacgaata ataaaaagta attgaagcgg cttaacgatg aaaagtaaat tgatgcgcat
     5041 accttaccaa aaggatgcat caatcgatat cgtcgttaag ctgttttggt ttacgtttca
     5101 tggattctac cccaattttc ataaatataa aaattccacc accaacatca aaattctcaa
     5161 catcgcaaca tacccaaatg ttataataaa tctattacac aaagagataa attacttatt
     5221 caaaggcgga ggaatcacat gtctattact gaaaaacaac gtcagcaaca agctgaatta
     5281 cataaaaaat tatggtcgat tgcgaatgat ttaagaggga acatggatgc gagtgaattc
     5341 cgtaattaca ttttaggctt gattttctat cgcttcttat ccgaaaaagc agaacaagaa
     5401 tatgcagatg cgttggcagg tgaagatatc acgtatcaag aggcatgggc agatgaagaa
     5461 tatcgtgaag acttaaaagc tgaattaatt gatcaagtcg gttacttcat tgaaccacaa
     5521 gatttattca gtgcgatgat tcgtgaaatt gaaacgcaag atttcgatat cgaacatctg
     5581 gcgacggcaa ttcgtaaagt tgaaacatca acattaggtg aagaaagtga aaatgacttt
     5641 atcggactgt tcagcgatat ggacttaagt tcaacgcgac taggtaacaa tgtcaaagaa
     5701 cgtactgctt taatctctaa agtcatggtt aatcttgacg atttaccatt tgttcacagt
     5761 gatatggaaa ttgatatgtt aggtgatgca tatgaattcc taattgggcg ctttgcggcg
     5821 acagcaggta aaaaagcagg cgagttctat acaccacaac aagtatctaa gatactggcg
     5881 aagattgtca cagacggtaa agataaatta cgtcatgtgt acgacccaac atgtggttcc
     5941 ggttcattat tgttacgtgt tggtaaagaa acgcaagtgt atcgttattt cggtcaagaa
     6001 cgtaacaata ccacttacaa cttagcacgc atgaacatgt tattacatga tgtacgttat
     6061 gaaaatttcg atatccgtaa tgatgacacg ttggaaaatc cagccttttt aggacataca
     6121 tttgatgcgg ttattgcgaa cccaccatac agtgcgaaat ggacagcaga ttcaaaattt
     6181 gaaaatgacg aacgattcag cggatacggc aaacttgcgc caaagtccaa agcagacttt
     6241 gcctttattc aacacatggt acattactta gacgatgaag gtaccatggc cgttgtactc
     6301 ccacatggtg tcttattccg tggtgctgca gaaggtgtca ttcgtcgtta tttaattgaa
     6361 gaaaagaact acttagaagc cgtgattggc ttaccagcca atattttcta tgggacaagt
     6421 attccaacat gtattttagt atttaaaaaa tgtcgccaac aagaagacta tgtattattt
     6481 atcgatgcat ccaatgattt tgaaaaagga aaaaatcaaa accatttaac cgatgcccaa
     6541 gtcgaacgca ttattaacac atataagcgt aaggaaacaa ttgataaata tagctacagt
     6601 gcgacattac aagagatcgc cgataacgat tacaacttaa acataccgag atatgtcgat
     6661 acattcgaag aagaagcacc gattgattta gatcaagtcc aacaagattt gaaaaatatc
     6721 gacaaagaaa tcgcagaagt tgaacaagaa atcaatgcat acctgaaaga acttggggtg
     6781 ttgaaagatg agtaatacac aaaagaaaaa tgtgccagag ttgaggttcc cagggtttga
     6841 aggcgaatgg gaagagaaga agttagggga ccttactacc aaaataggta gtggaaagac
     6901 tcccaaaggt ggaagtgaaa actatacaaa caaaggcata ccatttttaa ggagtcaaaa
     6961 tattagaaat ggtaaattaa atcttaatga cttagtttat attagtaaag atatagatga
     7021 tgagatgaaa aatagtagaa cgtactatgg tgatgttctt ttaaatatta caggagcatc
     7081 aataggtaga acagccatta attcgatagt tgaaatacat gctaatttaa atcaacatgt
     7141 atgtattatt agattgaaaa aagagcatta ttataatttt tttggacagt atctattatc
     7201 aagaaaaggt aaaaggaaaa ttttccttgc acaaagtgga ggtagtcgag aaggactaaa
     7261 cttcaaagaa attgctaatt taaaaatctt caccccaact atatttgaag agcagcaaaa
     7321 aataggcgaa ttcatcagca aacttgaccg acaaattgaa ttagaagaac aaaaacttga
     7381 attacttcag caacagaaaa aaggctatat gcagaaaatc ttctcgcaag aattgcgatt
     7441 caaagatgag ggaggtaaag attatccaga ttggaaatca aaatcaattc aagaaatatt
     7501 tgagaataag ggtggcactg ctctagaaac agaatttaat tttgacggta attataaagt
     7561 tataagtata ggaagttatt ctataaatag cacttataat gatcaa
//
LOCUS       DBNENW010000118         7593 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNENW010000118 DBNENW010000000
VERSION     DBNENW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.119"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..183)
                     /locus_tag="AC1LW8_002062"
     CDS             complement(<1..183)
                     /locus_tag="AC1LW8_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773869.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLS"
     gene            complement(202..1878)
                     /gene="kdpA"
                     /locus_tag="AC1LW8_002063"
     CDS             complement(202..1878)
                     /gene="kdpA"
                     /locus_tag="AC1LW8_002063"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIP4773870.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(1902..2012)
                     /gene="kdpF"
                     /locus_tag="AC1LW8_002064"
     CDS             complement(1902..2012)
                     /gene="kdpF"
                     /locus_tag="AC1LW8_002064"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIP4773871.1"
                     /translation="MLLCCKEVMTMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            2150..4807
                     /locus_tag="AC1LW8_002065"
     CDS             2150..4807
                     /locus_tag="AC1LW8_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIP4773872.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGIHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKVEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDV"
     gene            4807..5502
                     /locus_tag="AC1LW8_002066"
     CDS             4807..5502
                     /locus_tag="AC1LW8_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP4773873.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(5871..7391)
                     /gene="cshA"
                     /locus_tag="AC1LW8_002067"
     CDS             complement(5871..7391)
                     /gene="cshA"
                     /locus_tag="AC1LW8_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="degradosome RNA helicase CshA"
                     /protein_id="HIP4773874.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT         2673 a         1335 c         1122 g         2463 t
ORIGIN      
        1 cgatagataa ctagttccaa atatgtcagg aaaacatatt aatatcaacg ttaaaatcat
       61 tccaacctca acgacaaaca ttatcggatt ttttattaat tgtttaggat tcagcttata
      121 aaaactcatt ttcaaagctt ctatcaccat tgtttgattg aaatatttat ttacatgatg
      181 cattttttcc atccctttac tttattttaa agttaaaaat tcaccaatag gaccaagtaa
      241 tagtactgga ataaatgtca aaccacttag taaaacgata aatacgatta gtgatacgcc
      301 aaaataaggt ttatcaatcg ctattgtata tttatcttgg tggtatgatt ttttattcac
      361 taaacttgat gcaatcatta attgcaaaat aattggtata taacgagaaa gcaacataat
      421 gattcctgta gagatattcc agaatgttgt atcatctttc agtccttcaa accctgatcc
      481 attgttcgca gcagctgatg tcatttcata cataacttgc gaaataccat gaaaagacgg
      541 attcgttata ctttcacttg ctccaggaat cataaaagca agtgctgaaa atactaaaat
      601 taaaattgga tgtatgagaa agactaagac aatacatttc atttcacggg cgccaattgg
      661 catatttaaa tattctggtg ttttaccaac catcaaactg catataaaca ccgtcagtaa
      721 gacatatatc aataaattca tgagtcctac gccttcgcca ccaaatacaa catttagcat
      781 cattaatacc attggtccta atccacctaa aggcgttaag ctatcatgca tgttattaac
      841 agaacccgtt gtaaatgccg tcgtaataac tgtaaatagt gctgacaagc ctgctccaaa
      901 ccgtacctct ttaccttcca tattcggtcc ataaatgcct aaattcgcta gtattggatt
      961 accacgatac tcactccaca tagttaatgt aagaattgct ataaaaatga aaaacattgc
     1021 gacaaataat atcaacgcat gacgatgtac tcgtttacca tgtctactta acatgcgacc
     1081 aaataagaac aacattgaca taggaagtaa catcatactg cccatttcta taaaattgct
     1141 ccaaatattt ggattttcaa aaggtgttgc agaatttcct gctaaaaatc ctccaccatt
     1201 cgtaccaaga tgttttattg attcaagtga tgcaataggt ccaaatgcaa tatgttgaat
     1261 atgtccgctt aaagtccgaa tcattaaatt agcatgcaac gtttgtggta caccttgagt
     1321 catcaataaa atactaatta aacatgataa tggtaaaagt actcggacaa taaaccgaac
     1381 aatatcttga taaaaattac caatgatatt agttaatcca gttaaacgtc tcaacatcgc
     1441 tatacaaacg gcgtaacctg atgcactaga tgtaaacatt aaatatgtca ttacaatcat
     1501 ttgcgttaaa tatgtcacat ctgattcacc gttatagtgt tgtaaattac tatttgttaa
     1561 aaaagatatt gctgtattaa acgctaaatc tatcgattgg tttaaattat gatttggatt
     1621 taaaaaaagc cattgctgaa ctattagcaa tacaaatgtt ataaacccca taaatccatt
     1681 aaatgccaga aaatgtttga catatgtttt agctgacatg tgttctaaat ctgtgccgat
     1741 aattttaaaa cacatatttt caaatctagt aaatattaaa tctactcttg acgattgcac
     1801 caatgctacg cgatatagat atccactaaa aacatacgta atcataacca tcattgttag
     1861 aaacaaaatt atttccatga taaccctcac ttaatatatt tctaaaattt ttcactacga
     1921 attaaggcat aaaataaata caaaactaat gcaataacta ccagtaataa aacgatgagc
     1981 attgtcataa cctccttaca acacaacaac atcgtaacaa cttgtttatg agagaaatat
     2041 taattttcaa acttagttat taagaaatca ttaagatgtg tatgcagaaa taaattttat
     2101 agcatttaat tgtgaagaat attatgatat tgctatcgag gtgaaggtta tgtcaaacac
     2161 tgaatcgcta aacataggaa aaaagcgtgg atctttaaca atttacatcg gttacagtcc
     2221 aggtgttggt aaaacatttg agatgctttc aaatgccatt gaactatttc aaagtaacgt
     2281 tgatattaaa ataggatata ttgaaccgca tcagcgtgat gaaacaaatg cattagctga
     2341 acaattacct aaaatcacaa ccaattttac taaacatggt agtcatcatt ttcaatattt
     2401 agatgtcgac cgcataatcg aagaatcgcc gacaatagta cttatcgatg agttagcaca
     2461 tacgaatatt tctagagatc gtcatgagaa acgatatatg gatattgaag aaattttaaa
     2521 tcatggtatc gatgttcata ccactttgaa cattcaacat attgaaagtt taagtagtca
     2581 aattgaactg atgaccggta tacatgttaa agaacgtgta cccgactatt tcataatgag
     2641 cgccgatgta ttagaagtcg tagatatctc acctgaacaa ttaattaaac gcttaaaagc
     2701 tggcaaggta tataaaaagg ataggctaga tgtagcattt agtaatttct ttacgtatgc
     2761 ccacctaagc gaactgcgta cattgacgtt aagaacagtt gccgacttga tgagtgataa
     2821 agaaaaagtc cgacacaatc ataaaacgtc actcaaacct catattgctg tggcaattag
     2881 tgggagcatt tataatgaag cagtaattaa agaggcattc catattgctc aaaaagaaca
     2941 tgcgaaattc actgctattt atatagatgt attcgaaaaa aacaggcaat ataaagatag
     3001 tcaaaagcaa gtgcatcaac atctcatgct tgcaaaatca ttaggagcaa aagtaaaagt
     3061 agtttatagc caaaccgttg cattaggatt agacgaatgg tgtaaaaatc aagatgtaac
     3121 caaattaatt atcggacaac atattagaaa taagtggcga gactttttca atacaccttt
     3181 aattgaccat ttaatgtcct ttgaacatag ctataaagtc gaaatcgttc caatcaaaca
     3241 aatacctgtt gaattgaaaa tgaacaaatc accctatcgt cctaaaggca aacgtttcgc
     3301 catagatatg ttaaaaatga ttttgattca aataatttgt gtaatgatgg gactgtggat
     3361 ttatcaactt gataagcatg agtctagtac gattatttta atgatttttc tcatcggcat
     3421 cattttgtta tccatttgga cgcgatcctt catcattggc tttttagcag caattattaa
     3481 cgtatttgtt tttaattatt tttttacgga acctagatat acatttgaag tatatcgctt
     3541 tgactatcct attacattta tcgttagcat tttaacaagt attttaacga gtgctttatt
     3601 aaaacaaatt aaattccaat actccattac taaaaagcaa ctttatcgta cagatttatt
     3661 atttcaattt aatgattcga ttaaacaaac ttatacagtt gaaaacttac taataaatgc
     3721 tggataccaa attaatcaat tattgcaaca atcaattact atatacgtta tcaatcaatc
     3781 gaaagtaatt aaaacaatac cattgcaaaa ccatatcgat aatacgactc aacagcatga
     3841 acaagcatta agctgggtaa ttaaaaatga aagacaagct ggtgcaacaa cagatacatt
     3901 tccaggcatt aataaatggt tgataccaat tggcacatct ccgattaaag gcattctagc
     3961 cattgattac caaagttctc aagtgattaa tccatatgat gcatcaatat tagaatcaat
     4021 gttaaatgaa ctttctctcg ccgttgaaaa tgtgacgtta cttaaacaaa cgagagaatc
     4081 catgttacag gctgaacgtc aactgacaca ttcaaatttt ttaagatcaa tttcgcatga
     4141 catacgtaca ccgttaacaa caattatggg aaatttagat attttagtat cacatagtaa
     4201 agatatgtct atcatagaaa aggaacaatt acttgtgcat agttttcaag aaagtcagta
     4261 tttatatcta ttagtgacaa atattttatc attaacaaaa ttacagtcat caaatgttca
     4321 gataaaattg caaccttatc tcgttagtga attagtagaa gaaatcgata tgattctaga
     4381 acgtcgccat ttaaaaaagc gtataactgt atcatcctca gtagatttac agtttataca
     4441 cattgacagt aagttgattt tacaagcttt attcaatttg attgaaaatg cagttaaaca
     4501 cacttctact gacaccaaaa tcaatttatc tattcgttat gctagcgatg aacaaatcga
     4561 atttgccgtt atagacgaag gacctggaat tagtttagaa gaacaacaaa agatattcga
     4621 gcctttttac acaggatcaa ataaatattt taaagataac caaaaagaaa gcatgggact
     4681 aggcttatat ttagtacaga ctatcctaca caaacatcaa tcaaacttac aatataaacc
     4741 gaaccaacca cacggcagta tattctattt caatatagat acagacttta atgaaggaga
     4801 cgtataatgc aatctaaaat attgataatt gaagatgatc acgcaatcac acatttactt
     4861 gatgttgcat taactttaga ttattacaat gtaactacag ccgacaatgc cacacaagca
     4921 cactttaaaa ttcaaattga taaaccagat gtcattttat tagatttagg tttaccagat
     4981 aaagatggat tatgtttgat ttcagaaatc aggcaacata ctgacattcc tatcattgta
     5041 ataagtgcaa gacaagaaga acaaacaatt attcaagctt tagataacgg tgcgaatgac
     5101 tatatgacta aaccttttaa tgttgatgag cttcgggcac gaatacgagt catcgagaga
     5161 atcgctaagt cgcatcaaga aactaacatt gtatttacta acggtttgct aagcatcgac
     5221 tttggctcca aatctgttgt tattaataat caggaggtac atttgactcc aaatgaattt
     5281 agcttgttag aactattgtc aaatcataaa ggaaaagtac tcacttatga gatgatatta
     5341 aaacgaatat atggctatgt taataagact gaaatgccta gtttaagagt gcatatgaca
     5401 tcattaagac aaaaactttc acaatgtcat gaggacgcaa aagatattat taaaacacat
     5461 ccacgcattg gttatcaaat gttgcagtgg aaagagaaat aattaaataa aaaagatcct
     5521 gccattaaac gaatgattgg cagtgatctt ttttatattc aacttattat ttatcgcgca
     5581 ctagcgtcag caaaaagccc tattattttt ttacaagaac atgatactga aatttcataa
     5641 agtttattta tgttcgatta ttcttaattt aaaaatagtt ctttaaatta tatactcacc
     5701 acatttggtg gagaaccata tttacacaga atgatataac cgcatacaga tctcaatcca
     5761 attaaatatt taacctttaa aacgttagta ctttgatatc tacagagaat caattattga
     5821 aaacaaaaaa gagctcaaga cattacatct ttaagctctt aatctataaa ttatttttga
     5881 tggtcagcaa atgtgcgacc tttcataggt ctgcttccac cgctgctctt ctctttacgg
     5941 tcgaattttt ttgtactttt ctttttactt gagtatcctt ttgaacgttt actcttactg
     6001 tcaaatttag gattaccacg cttactattt ctgttacgag aaccgcttgg tttaccgtta
     6061 cggcctttgc gagataatgg tttttcaaaa gttaattgaa cttcaacttc atcgttcgct
     6121 tctactaact cttgtaaaag tgcagcaact aaatcaacat cgttatattc atttaacaac
     6181 tctgtagaaa tgcgtttcaa gcgtgattct gactctttag acatccagtt ttcaactttt
     6241 tctttgatgt catcttcacg tgcttgaagt acttctttac gatgaggtgg acgaagtgcg
     6301 ctcatttttc taccattagc atcttcaatt tgtctaatgt aatccatttc aattggattt
     6361 acaaatgtta ccgcgatacc ttctttacca gcacgacccg tacgaccaat acggtgtgta
     6421 tagctttcag tatcttgagg tatatcaaag ttataaacat gactcacacc agaaatatct
     6481 agtcctcttg ctgctacatc agtagcgact aaaatattaa tttggtcatt tttaaatttc
     6541 tttaatactt ctaaacgttt cgcttgtgta atatcaccat gtaagccttc agctttatat
     6601 cctttagaaa tcaaagcact tgttaattca tcaacacgac gttttgtacg tccgaatacg
     6661 attgctaatt caggttgatg aacatctagg aaatttgtaa atgtatcaaa tttctctaat
     6721 tctttaacaa ttgtatagaa ttcttcgatt tgtggatcag acatttcatt attcattgtc
     6781 ttaatgattt ttggtgattt cataaattgt tgtactaaag cttggattgc tttaggcatt
     6841 gtagctgaga acaacattgt ttgacgttgt actgctggaa ttttatccat aataaatctc
     6901 atatcatcga tgaatcccat attcatcatt tcatcagctt catctaaaat caaagtatga
     6961 attccgtccg tttttaatgt gcgacgattt aaatggtcga taacacgccc aggtgttccg
     7021 actacgattt gtgggccttt tttcaaggct ttaatttggc gttcgatagg cataccaccg
     7081 aatacagtaa caacttggac accttgtcca cggctaaatt ctcttaattg ttcagctacc
     7141 tgcattgcca attctcttgt aggtgctaaa atcaacgatt gaaccccttg tttccctact
     7201 actttctcaa ttaaaggaat accgaatgct cctgttttac ctgtaccggt ttgagcttgc
     7261 ccaaggatat caattccttg taacgcataa gggatactgt ctttttggat aggtgtcggc
     7321 tctttaaatc ccattgattc aagtgactga accgtattat ccgaaatccc tagttcttta
     7381 aaattttgca aaataattct cctttttacc tgtttatttc tcaatatact tttgcatata
     7441 agttcattaa tatttcatca acttctatat agtaccacca aaaaaattta atcgcaataa
     7501 agcaatttgc gaatatttat tagaatcaca ttaattgatt gtcatatttt tgtcaaaaag
     7561 ctagcattag ataagatgta agcgttacgc atc
//
LOCUS       DBNENW010000119         7554 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNENW010000119 DBNENW010000000
VERSION     DBNENW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.120"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(76..324)
                     /locus_tag="AC1LW8_002068"
     CDS             complement(76..324)
                     /locus_tag="AC1LW8_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIP4773875.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            432..1385
                     /locus_tag="AC1LW8_002069"
     CDS             432..1385
                     /locus_tag="AC1LW8_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP4773876.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            1375..2754
                     /locus_tag="AC1LW8_002070"
     CDS             1375..2754
                     /locus_tag="AC1LW8_002070"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIP4773877.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            2907..4367
                     /gene="ebpS"
                     /locus_tag="AC1LW8_002071"
     CDS             2907..4367
                     /gene="ebpS"
                     /locus_tag="AC1LW8_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HIP4773878.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKVSQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            4492..4608
                     /locus_tag="AC1LW8_002072"
     CDS             4492..4608
                     /locus_tag="AC1LW8_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773879.1"
                     /translation="MLFNKVLSRILAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            4769..5755
                     /gene="ypdA"
                     /locus_tag="AC1LW8_002073"
     CDS             4769..5755
                     /gene="ypdA"
                     /locus_tag="AC1LW8_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol disulfide reductase YpdA"
                     /protein_id="HIP4773880.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGDIIAQSMLAKKQTPLES"
     gene            complement(5870..6838)
                     /locus_tag="AC1LW8_002074"
     CDS             complement(5870..6838)
                     /locus_tag="AC1LW8_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIP4773881.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDTNPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            6915..>7554
                     /gene="cmk"
                     /locus_tag="AC1LW8_002075"
     CDS             6915..>7554
                     /gene="cmk"
                     /locus_tag="AC1LW8_002075"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIP4773882.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILA"
BASE COUNT         2824 a         1194 c         1311 g         2225 t
ORIGIN      
        1 taataaaaag caggtgacta tcaataaacg atagcttgcc tgctttttct atagaacatt
       61 tgtctaaaaa atcaattatt caaattttaa agcgtcccca tcaaatgatt cgtctgcaat
      121 tttaatagaa tctgtagggc atccatcaat tgcatcttcc atatcttcat ataattcctc
      181 aggtacttct gcagtacctt ggttatcgtc aaggattacg aaagcaatac cttcgtcgtc
      241 gtaatcatat atatctggcg ctgctgcacc gcatgcacca catgcaatac aagtatccat
      301 atcaacgatt gtatattttg ccaatgtctt cgcctccttt gataaaaatg ctaaaatagt
      361 aatgtgacta aaattttaga cagcatcatt tttattttca aattatccgt tttacagagt
      421 gagggttaaa tttgcaacac attataaaaa cagcattaca acaaacattt aactataaaa
      481 caaataaaag tatttacaat atcttagttg gtaagaaatc tcaccaaacc ttttttgacg
      541 cttgtagtca acaacagttg tcattatatc acagtttacc actattaaaa tatccgtctt
      601 ttgagctatt tttagaaaaa atcaatgaat ttaatgctga aatggaaatc atgttgcatc
      661 ctagatatac atttgaaagc atggggcaaa catttcaagc aattcaacta ttagtgcaaa
      721 ccatgtctaa taccaaacaa catgtttttc attttgtacc aatctctcaa aataataaga
      781 tacaagaaac tgtaaagatt gtctataact acattaaaga aaaccaatta caaattgatt
      841 ttgaaaatga actacataac ttgtttaaag caataacttt aaaagggcca tgttacttac
      901 attattattt gcaaggctat gatgaaccaa tgtatacgag acagcaagtt agtttaatag
      961 aaaagctatc tcaacagcaa ttgtttgaat acgaaatgaa taatttagtg acaatgatgt
     1021 ttgaattaga aagtggagaa tatactattt tatcaaaaat aataatgaaa cctacattat
     1081 taaatcaaac ttatattact tatacaaaat tgcttgaaca attcacgatg gaagatatag
     1141 cggctcaaca acaagttaaa atcaatacta tcgaagatca tgtacttgaa atcttaatca
     1201 aaggttacat gtctaattac gatgattatg ttgaactaga agatcaactc cagtttttga
     1261 atttttatca acagcatcgt ggcgaacgat taaaatttta caaagaacaa tttgacacgt
     1321 tatcatattt tcaattaaaa gtattaatcg ttggatttga aagaggtgat ctgaatgttg
     1381 catgatattt tacgaaacaa atttggattc gagagtttta aaccgggaca acaggaaatt
     1441 atagaaagta taatgtctca acaacacact ctaggtatac ttccaactgg aagtggaaag
     1501 agtttgtgtt atcaaatacc tacgtattta tcaggtaagc cgacattaat tatctcaccg
     1561 ttaatatctt taatggatga ccaagttatg cagttgaaaa taaatggaga aaaacgtgta
     1621 acatgtattc actctggtat ggatgaaatt gagaaaaagc ataatattaa atgtttacga
     1681 catagccgct tcatctttct aagtccagaa tttctcttgc aaccgtcaaa ttttaaatta
     1741 atatctatga tagactttgg catgattgtt ctagatgaag cacattgcct atctgaatgg
     1801 ggatatgatt tcagaccaca ttatgctcta ataggaaaag taacaaagca ttttaaagaa
     1861 gcggttgtct tagcattgac agcaactgca ccaccgcatt tacaagatga tttgacggaa
     1921 atgttagcga ttcaatttaa cgttattaaa actacaatga atcgcccaaa cataagcttt
     1981 aagcatctta attttcatga tgacgaagat aaaattgagt ggttgctgcc gtttctacaa
     2041 cagtcgggac caacgattat ttatgtctca tcgaaaaaga tgtgtctgaa tttagcgcaa
     2101 cttatttatg attcaggttt tcttacaggt atttatcatg gtgatatgaa ttatcaagag
     2161 cgacacacag ttcaacaaca atttttaaat aatgatattc cgattatagt cgcaacgagt
     2221 gcttttggta tgggaattaa taaaaaagat attcgcacaa tcattcactt tcatctttca
     2281 acaagtcctt ctaactacat tcaagaaatt ggccgtgcgg gtcgcgatgg tgaactaagt
     2341 caggcaatta gtttattcca accggacgat aaatatattt tagaaacgtt attatttgca
     2401 gatatgataa cagaagaaga tgtacaaaat ttcgaaatag gagaattttt agctcccgat
     2461 aaacaagacg ttttgacaac gttgcattca ttctatagta tcggcgcctt gaaacagata
     2521 tttaagcaat catttaaacg aaagcaatta ggattctttc gcatgattgg ctattgcaaa
     2581 ttggatcaat gtagacggaa gtatttatta gaatttttcg gtgaatatcc accggcacaa
     2641 gatcgatgtt gtgacaatga ttctaatata actgatatcg caattttaaa taagaagaag
     2701 gtaattagaa gtattggatt tgatgaaaag ttgcaaaatt tatttctcag atagtattac
     2761 tttactaaaa gaaaattgac aagctataat tagtgtatac acaattgaaa aatgattgaa
     2821 ataattttga aaaatataca taaacatatg tcatgtgggt atattttatg taaaatcatt
     2881 gtaatagaat agaaaggaag atggctatgt ctaataattt taaagatgac tttgaaaaaa
     2941 atcgtcaatc gatagacaca aattcacatc aagaccatac ggaagatgtt gaaaaagacc
     3001 aatcagaatt agaacatcag gatacaatag agaatacgga gcaacagttt ccgccaagaa
     3061 atgcccaaag aagaaaaaga cgccgtgatt tagcaacgaa tcataataaa caagttcaca
     3121 atgaatcaca aacatctgaa gacaatgttc aaaatgaggc tggcacaata gatgatcgtc
     3181 aagtcgaatc atcacacagt actgaaagtc aagaacctag ccatcaagac agtacacctc
     3241 aacatgaaga ggaatattat aataagaatg cttttgcaat ggataaatca catccagaac
     3301 caatcgaaga caatgataaa cacgagacta ttaaagaagc agaaaataac actgagcatt
     3361 caacagtttc tgataagagt gaagctgaac aatctcagca acctaaacca tattttgcaa
     3421 caggtgctaa ccaagcaaat acatcaaaag ataaacatga tgatgtaact gttaagcaag
     3481 acaaagatga atctaaagat catcatagtg gtaaaaaagg cgcagcaatt ggtgctggaa
     3541 cagcgggtgt tgcaggtgca gctggtgcaa tgggtgtttc taaagctaag aaacattcaa
     3601 atgacgctca aaacaaaagt aattctgaca agtcgaataa ctcgactgag gataaagtgt
     3661 ctcaagataa gtctaaagat catcataatg gcaaaaaagg tgcagcgatc ggtgctggaa
     3721 cagcaggttt ggctggaggc gcagcaagta aaagtgcttc tgccgcttca aaaccacatg
     3781 cctctaataa tgcaagccaa aaccatgatg aacatgacaa tcatgacaga gataaagaac
     3841 gtaaaaaagg tggcatggcc aaagtattgt taccattaat tgcagctgta ctaattatcg
     3901 gtgcattagc gatatttgga ggcatggcat taaacaatca taataatggt acaaaagaaa
     3961 ataaaatcgc gaatacaaat aaaaataatg ctgatgaaag taaagacaaa gacacatcta
     4021 aagacgcttc taaagataaa tcaaaatcta cagacagtga taaatcaaaa gaggatcaag
     4081 acaaagcgac taaagatgaa tctgataatg atcaaaacaa cgctaatcaa gcgaacaatc
     4141 aagcacaaaa taatcaaaat caacaacaag ctaatcaaaa tcaacaacag caacaacaac
     4201 gtcaaggtgg tggccaaaga catacagtga atggtcaaga aaacttatac cgtatcgcaa
     4261 ttcaatacta cggttcaggt tcaccggaaa atgttgaaaa aattagacgt gccaatggtt
     4321 taagtggtaa caatattaga aacggtcaac aaatcgttat tccataatat aactatataa
     4381 attgtaactg aactgctata tacaaacgtg catattaaaa gtatctctaa atattttgga
     4441 gaacttaaat tgcgcttata agtatgtagc ggttttttca tttttcaaag tttgttattt
     4501 aacaaggtgt tgtctcgaat attggcatat caattaaatt ttttacatag tcatcaaaaa
     4561 gataaaacac cacaatcaac aaatttaacg aggaagaata aaaaataaat caacatatta
     4621 aattgtagtg ttattcaact ccgtagctaa caattctcta tgcatattaa acaaattgtc
     4681 gaaaatatat cataaatctt caagcacaga cttagcgcat caatcactga actgttataa
     4741 tagtttggga ttaaaggagg ccgaaacaat gcaaaaagtt gaaagtatca taattggtgg
     4801 agggccatgc ggattaagtg cggctattga acaaaaaaga aaaggtattg ataccttaat
     4861 tattgaaaag ggtaatgtcg ttgaatcaat atataattat cctactcacc aaacattttt
     4921 ctcatcaagt gataaattaa gtattgggga cgtaccgttt atcgttgaag aaagtaaacc
     4981 aagacgtaat caagcgctag tttattaccg agaagttgta aaacatcatc aattaaaagt
     5041 aaatgcattt gaagaagtat taactgttaa aaaaatgaat aataaattta caattactac
     5101 gacgaaagat gtttatgaat gtcgattttt aacaatcgcg acaggctatt atggtcagca
     5161 taatacatta gaggttgaag gtgcggattt acctaaagtg ttccattatt ttaaagaggc
     5221 acatccgtat tttgatcaag atgttgttat tattggtggt aagaattcgg ctatcgatgc
     5281 tgctttggag ttggaaaaag ctggtgctaa cgtgacggtt ctatatcgtg gtggagatta
     5341 ttcgccttca attaaaccgt ggatacttcc aaatttcaca gcattagtaa atcatgaaaa
     5401 aattgacatg gaatttaatg ctaatgttac ccaaataact gaagatactg tgacttatga
     5461 agtaaatggt gaaagtaaaa cgatacacaa tgattatgtt tttgcgatga ttggttatca
     5521 tcccgattat gaatttttaa aatctgtagg tattcaaatt aatacaaatg aatttggaac
     5581 agcgcctatg tataataaag agacatacga aacaaatatc gaaaattgct acattgcagg
     5641 tgtgattgct gcagggaatg atgcgaatac catttttatt gaaaatggta aattccacgg
     5701 tgacattatt gctcaaagca tgctagctaa gaaacaaacg ccgttagaat cataaaaata
     5761 aaggtctatg tactaaatat cttagttata caacgactga cattcatgat atgtcaaatg
     5821 aggttgatga ctaatgattg tacatagacc tttttatgtt acgtaatcat tataattcaa
     5881 aatatgattt gatttcagct ttatctaaat tgttgcttaa cgcaactaat aattttaatc
     5941 ttgcttttgg accattcaaa ccgttagaaa aaataaaacc ttgttgtgcg agttggtaac
     6001 caccaccatc gtatgcgtaa gttggactca caataccatt aaaggaacgt gaaactagca
     6061 caataggtat atttaaagat actaattgtt gaatgccttc taatgcgctt ggaggtatgt
     6121 tgccttgtcc taacgcttca ataaccatac catcgatacc ttctcgacta taaaaactaa
     6181 aaatgtcacc tggcataccc atatatgctt ttactaatgg tacatttagt ttgtcattga
     6241 cattttccaa tgcttgttgg cgatatggca tatgatggaa ttgcacacga tcctttgtca
     6301 atacacctag cggaccatga tttggacttt gaaatgtgtt tgtattagac gtatgtgttt
     6361 tggtaacatt acgcgccgta tgaatttcat cattaaatac aaccatcacg cctttatgac
     6421 gggccttttc atcagaggca acgcgaatag cggaaatata attatataat ccgtcagaac
     6481 caatttcatt agacgagcgc attgcgccag taataacaac aggttgctcg atacctaata
     6541 ttaaatcaag taaaaaggca gtttcttcta acgtatcggt accatgcgtg ataacgaaac
     6601 catcataata tttatttgtt actgcttcta aaataatatc ctttaattgt ttaacatgtt
     6661 ggattgtcat atgaggtgat ggtacattaa aaggatttaa ttcatctatt tgtgcatatt
     6721 gatttatgac atcttgatgc attgaaatag ggttggtatc atttgttact actttattag
     6781 attggtcttg tgacatacta atggtgccac cagtatgaat aacaagtaga tgtttcatat
     6841 atttcctcct atatttaatt tacctaatta tgataaaata ttattcataa aacgacaagg
     6901 aagggaaatg acgcatgaaa gccattaata ttgcattaga tggtccagct gctgccggaa
     6961 aaagtacaat tgcgaaacgt gtagccagcg aactatcaat gatttatgtc gatacaggag
     7021 caatgtatcg tgcattaaca tacaaatatt taaaattaaa caaaactgag gactttgcaa
     7081 aactagttga ccaaacaaca ttagatttaa cttataaagc agataaaggt caatgtgtca
     7141 ttttagataa cgaagatgta acagactttt taagaaataa tgatgtgacg caacatgttt
     7201 catacgttgc atctaaagag ccagtacgtt cattcgccgt taaaaaacaa aaagagttag
     7261 ctgcagaaaa aggtatcgta atggatggtc gcgatatcgg aactgtagtg ctaccagatg
     7321 cagatttaaa agtatatatg attgcatcag ttgaagagcg agcagaaaga agatataaag
     7381 ataatcaatt aagaggtatc gaatcaaatt ttgaagattt aaaacgtgat attgaagctc
     7441 gtgatcaata tgacatgaac cgtgaaatat caccattaag aaaagcagat gatgcagtga
     7501 cattagatac gacaggcaag tcgattgaag aagttactga cgaaatttta gcga
//
LOCUS       DBNENW010000120         7539 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNENW010000120 DBNENW010000000
VERSION     DBNENW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.121"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..609
                     /gene="capA"
                     /locus_tag="AC1LW8_002076"
     CDS             <1..609
                     /gene="capA"
                     /locus_tag="AC1LW8_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HIP4773883.1"
                     /translation="LIILPLLFLIISAIVTFFVLSPKYQANTQILVNQTKGDNPQFMA
                     QEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINIQV
                     KSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFF
                     LGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            625..1311
                     /gene="cap8B"
                     /locus_tag="AC1LW8_002077"
     CDS             625..1311
                     /gene="cap8B"
                     /locus_tag="AC1LW8_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HIP4773884.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIASERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1314..2078
                     /gene="cap8C"
                     /locus_tag="AC1LW8_002078"
     CDS             1314..2078
                     /gene="cap8C"
                     /locus_tag="AC1LW8_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HIP4773885.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGRKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2098..3921
                     /gene="cap8D"
                     /locus_tag="AC1LW8_002079"
     CDS             2098..3921
                     /gene="cap8D"
                     /locus_tag="AC1LW8_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HIP4773886.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHAPLMEENPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3911..4939
                     /gene="cap8E"
                     /locus_tag="AC1LW8_002080"
     CDS             3911..4939
                     /gene="cap8E"
                     /locus_tag="AC1LW8_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HIP4773887.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4952..6061
                     /gene="cap8F"
                     /locus_tag="AC1LW8_002081"
     CDS             4952..6061
                     /gene="cap8F"
                     /locus_tag="AC1LW8_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HIP4773888.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6065..7189
                     /gene="cap8G"
                     /locus_tag="AC1LW8_002082"
     CDS             6065..7189
                     /gene="cap8G"
                     /locus_tag="AC1LW8_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HIP4773889.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKKLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7192..>7539
                     /gene="cap8H"
                     /locus_tag="AC1LW8_002083"
     CDS             7192..>7539
                     /gene="cap8H"
                     /locus_tag="AC1LW8_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HIP4773890.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEH
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENRYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILN"
BASE COUNT         2761 a         1066 c         1470 g         2242 t
ORIGIN      
        1 ttaattattt taccgctatt atttttaatt attagcgcta ttgttacatt tttcgtctta
       61 tcacctaaat atcaagctaa tactcaaatc ttagtgaatc aaactaaggg tgacaatcct
      121 cagtttatgg cacaagaggt tcaaagtaat attcaacttg taaatacgta taaagaaatt
      181 gttaaaagtc ctagaatttt agatgaggtg tcaaaggact taaatgataa gtattcacca
      241 tctaaattgt cgagtatgtt gacaattaca aaccaagaaa atacgcaact tatcaacatc
      301 caagttaaaa gtggtcataa acaagattcg gaaaaaattg cgaatagctt cgctaaagtt
      361 acaagtaaac aaattccgaa gattatgagt gtggataacg tatcaatttt atctaaagca
      421 gacggtacag cagttaaagt cgcaccaaaa actgtagtga atctaatcgg tgcattcttt
      481 ttaggattag ttgtcgcgct tatatatatc ttcttcaaag taattttcga taagcgaatt
      541 aaagatgaag aagatgtaga gaaagaatta ggattgcctg tattgggttc aattcaaaaa
      601 tttaattaag gatggttgct acttatgtca aaaaaggaaa atacgacaac aacactattt
      661 gtgtatgaaa aaccaaaatc aacaattagt gaaaagtttc gaggtatacg ttcaaacatc
      721 atgttttcaa aagcaaatgg tgaagtaaag cgcttattgg ttacttctga aaagcctggt
      781 gcaggtaaaa gtacagttgt atcgaatgta gcgattactt atgcacaagc aggctataag
      841 acattagtta ttgatggcga tatgcgtaag ccaacacaaa actatatttt taatgagcaa
      901 aataataatg gactatcaag cttaatcatt ggtcgaacga ctatgtcaga agcaattacg
      961 tcgacagaaa ttgaaaattt agatttgcta acagctggcc ctgtacctcc aaatccatct
     1021 gagttaatag cttcagaaag gtttaaagaa ttagttgatc tgtttaataa acgttacgac
     1081 attattattg tcgatacacc gccagttaat actgtgactg atgcacaact atatgcgcgt
     1141 gctattaaag atagtttgtt agtaattgat agtgaaaaaa atgataaaaa tgaagttaaa
     1201 aaagcaaaag cacttatgga aaaagcaggc agtaacattc taggtgtcat tttgaacaag
     1261 acaaaggtcg ataaatcttc tagttattat cactattatg gagatgaata agtatgattg
     1321 atattcataa ccatatattg cctaatatcg atgacggtcc gacaaatgaa acagagatgc
     1381 tggatctttt aaaacaagcg acaacacaag gtgttacaga aatcattgta acatcacatc
     1441 acttacatcc tcgatatacc acacctatag aaaaagtgaa atcatgttta aaccatattg
     1501 aaagcttaga ggaagtacaa gcactaaatc taaagtttta ttatggtcag gaaataagaa
     1561 ttaccgatca aatccttaat gatattgatc gaaaagttat taccggtatt aatgattcac
     1621 gctatttact aatagaattt ccatcaaatg aagttccaca ctatactgat caattatttt
     1681 tcgaattaca gagtaaaggc tttgtaccga ttattgcaca tccagagcgg aataaagcaa
     1741 taagtcaaaa ccttgacata ctatacgatt taattaacaa aggtgcttta agtcaagtga
     1801 caacggcgtc attagcgggt atttccggta gaaaaattag aaaattagca attcaaatga
     1861 ttgaaaacaa tctgacacat ttcatcggtt cagatgcgca taacacagaa atcagaccgt
     1921 tcttaatgaa agacttattt aatgataaga aattacgtga ttattatgaa gatatgaacg
     1981 gatttattag taatgcgaag ttagttgttg atgataaaaa aattcctaaa cgaatgccac
     2041 aacaagatta taaacagaaa agatggtttg ggttataaac agcaaatgag gggttttatg
     2101 gcacatttat ctgtgaaatt gcggctttta atactagcat taatcgattc actgatagtg
     2161 acattttcag tattcgtaag ttattacatt ttagaaccgt atttcaaaac atattctgtc
     2221 aaattattaa tattggcagc tatatcacta ttcatatcgc atcatatttc agcatttatt
     2281 tttaatatgt atcatcgagc gtgggaatat gccagtgtga gtgaattgat tttaattgtt
     2341 aaagctgtga cgacatctat cgttattacg atggtggtcg tgacaattgt tacaggcaat
     2401 agaccgtttt ttagattgta tttaattact tggatgatgc acttgatttt aataggtggc
     2461 tcaaggttat tttggcgtat ttatcggaaa taccttggag gtaagtcatt taataagaag
     2521 ccaactttag ttgttggtgc tggtcaagca ggttcaatgc tgattagaca aatgttgaaa
     2581 agtgacgaaa tgaaacttga accggtatta gcagtcgatg atgacgaaca taaacgcaat
     2641 atcacaatta ctgagggtgt aaaagtccaa ggtaaaattg cggatattcc agaactagtg
     2701 aggaaatata agattaaaaa aatcatcatt gcaattccaa ctattggtca agagcgtttg
     2761 aaagaaatta ataatatttg ccatatggat ggcgttgagt tattgaaaat gccaaatata
     2821 gaagacgtca tgtctggtga gttagaagtg aaccaactta aaaaagttga agtagaagat
     2881 ttactaggca gagatcctgt tgaattagat atggatatga tatcaaatga attgacgaat
     2941 aaaactattt tagttacggg tgcaggtggt tcaataggat cagaaatttg tagacaagtt
     3001 tgtaatttct atccagaacg tattattcta cttggccatg gtgaaaacag tatttattta
     3061 atcaatcgtg aattgcgaaa tcgcttcgga aaaaatgttg atatcgttcc tattatagcg
     3121 gatgtgcaaa atagagcgcg tatgtttgaa attatggaaa cgtataaacc atacgcagtt
     3181 tatcatgcag cagcacacaa gcacgcgccg ttaatggaag agaaccctga agaagcagta
     3241 cgtaataata ttttaggtac gaaaaatact gctgaagctg ctaaaaatgc agaggtgaag
     3301 aaattcgtta tgatttctac ggataaagcc gttaatccgc ctaatgtcat gggagcttca
     3361 aagcgaattg cagaaatgat tattcaaagt ttaaatgatg aaacgcatcg aacaaatttt
     3421 gttgcagtga gatttggtaa tgtacttgga tcgagaggat ctgtgattcc acttttcaaa
     3481 agtcaaattg aagaaggtgg gccagttact gtgacacatc ctgaaatgac acgttacttt
     3541 atgacaattc ctgaagcttc tagactagtt ttgcaggcag gggcattagc agaaggtggc
     3601 gaagtatttg tgctagatat gggagaacca gtgaaaattg tagatttggc acgtaattta
     3661 attaagctaa gtggtaaaaa agaagacgac atacgcatta cttatacagg gattagaccc
     3721 ggcgaaaaaa tgtttgaaga gcttatgaat aaagatgagg ttcatcctga acaagtattt
     3781 gaaaaaattt atcgtggcaa agtacaacat atgaaatgta atgaagttga agcgattatt
     3841 caagacatcg tcaatgactt tagtaaagaa aaaattatta actatgccaa tggcaaaaag
     3901 ggagataatt atgttcgatg acaaaatttt attaattact gggggcacag gatcattcgg
     3961 taatgctgtt atgaaacggt ttttagattc taatattaaa gaaattcgta ttttttcacg
     4021 cgatgagaaa aaacaagatg acattcgaaa aaaatataat aattcaaaat taaagttcta
     4081 cattggtgat gtgcgtgata gtcaaagtgt agaaacagca atgcgagatg ttgattatgt
     4141 attccatgca gcagctttaa aacaagtgcc gtcatgtgaa ttctttccag ttgaggcagt
     4201 gaagacaaat attattggta cagaaaatgt cttacaaagt gctattcatc aaaatgttaa
     4261 aaaagtcata tgtttatcta cagataaggc agcgtatcct attaatgcta tgggtatttc
     4321 aaaagcaatg atggaaaaag tattcgtagc caaatcaaga aatattcgta gtgaacaaac
     4381 gctgatttgt ggtacaagat acggtaatgt gatggcttca agaggatcag taattccttt
     4441 gtttatcgac aaaatcaaag ctggagaacc tttaacgatt acagatcctg atatgacaag
     4501 atttttaatg agtttagaag atgccgtaga actagttgtt catgcattta agcatgcaga
     4561 aacaggcgat attatggttc aaaaagcacc aagctcaacg gtaggggatc ttgcgaccgc
     4621 attattagaa ttgtttgaag ctgataatgc aattgaaatc attggtacac gacatggaga
     4681 gaaaaaagca gaaacattgt tgacgagaga agaatacgca caatgtgaag atatgggtga
     4741 ttattttaga gtgccggcag actccagaga tttaaattat agtaattatg ttgaaaccgg
     4801 taacgaaaag attacgcaat cttatgaata taactccgat aatacacata ttttaacggt
     4861 ggaagagata aaagaaaaac ttttaacact agaatatgtt agaaacgaat tgaatgatta
     4921 taaagcttca atgagatagg agagattgac gttgaatatt gtaattacag gagcaaaagg
     4981 ttttgtagga aaaaacttga aagcagattt aacatcaacg acagatcatc atattttcga
     5041 agtacatcga caaactaaag aggaagaatt agagtcagca ttgttgaaag cagactttgt
     5101 cgtgcattta gcgggtgtta atcgacctga acatgacaaa gaattcagct taggaaacgt
     5161 gagttattta gatcatgtac ttgatatatt aactagaaat acgaaaaagc cagcgatatt
     5221 attatcgtct tcaatacaag caacacaaga taatccttat ggtgagagta agttgcaagg
     5281 ggaacagcta ttaagagagt atgccgaaga gtatggcaat acggtttata tttatcgctg
     5341 gccaaattta ttcggcaagt ggtgtaagcc gaattataac tcagtgatag caacattttg
     5401 ttacaaaatt gcacgtaacg aagagattca agttaatgat cggaatgttg aactaacgct
     5461 aaactacgtg gatgatatcg tcgctgaaat aaagcgtgct attgaaggaa ctccaacgat
     5521 tgaaaatggt gtacctacag taccaaacgt atttaaagtg acattgggag aaattgtaga
     5581 tttattatac aagttcaaac agtcacgtct cgatcgaaca ttgccgaaat tagataactt
     5641 gtttgaaaaa gatttgtata gtacgtattt aagctatcta cctagtacag actttagtta
     5701 tcccttactt atgaatgtgg atgatagggg ttcttttaca gaatttataa aaacaccgga
     5761 tcgtggtcaa gtttctgtaa atatttctaa gccaggtatt actaaaggta atcactggca
     5821 tcatactaaa aacgaaaaat ttctagtcgt atcaggtaaa ggggtaattc gttttagaca
     5881 tgttaatgat gatgaaatca ttgagtatta cgtttctggc gacaaattag aagttgtaga
     5941 cataccagta ggatacacac ataatattga aaatttaggt gacacagata tggtaactat
     6001 tatgtgggtg aatgaaatgt ttgatccaaa tcagccagat acgtatttct tggaggtata
     6061 gcgcatggaa aaactaaaat taatgacaat agttggtaca aggcctgaaa tcattcgttt
     6121 atcatcaacg attaaagcat gtgatcaata ttttaatcag atattagtac acactggtca
     6181 aaattatgat tatacattga atcaaatttt ctttgatgat ttggaattga gacaaccgga
     6241 ccactactta gaggcagttg gaagtaacct tggagaaacg atggggaata ttattgcgaa
     6301 gacatatgac gttttattac gcgaacaacc agatgcactt ttaattcttg gtgatacaaa
     6361 tagttgttta gcagcagtat ctgctaaacg attaaagatt cctgtgttcc acatggaagc
     6421 gggtaataga tgctttgatc agaatgtacc tgaagaaatc aatcgtaaaa ttgttgacca
     6481 tgtcagtgat gtgaatctac cttatacgga acatagcaga cgttatttat tagatgaagg
     6541 cttcaataaa gcgaatatct ttgtgacagg atcaccgatg acagaagtga tagaagcgca
     6601 tcgagataaa attaatcaca gtgacgtttt aaataaacta ggattagaac cgcaacaata
     6661 cattttagta tctgcgcata gagaagagaa tatcgataat gaaaagaatt ttaaatcatt
     6721 aatgaatgcg ataaatgata ttgccaaaaa gtataaaatg cctgtgattt attcaacgca
     6781 tccaagaagt tggaagaaaa ttgaagaaag taaatttgaa tttgatccat tagttaaaaa
     6841 gttaaagcca tttggtttct ttgattataa tgcattgcaa aaagatgcat ttgttgtgct
     6901 atcagatagt ggaacattgt cagaagagtc gtctattttg aagttccctg gtgtccttat
     6961 tcgaacttcc acagaaagac cggaagtact agataaaggt acggttattg taggtggtat
     7021 tacctataac aatctaatcc aatccgttga actagcaaga gagatgcaaa acaataacga
     7081 accgatgatt gatgctattg attataaaga cactaacgtt tcgacaaagg tagttaaaat
     7141 tattcaaagc tataaagata ttatcaatcg aaatacttgg aggaaatgac gatgaggata
     7201 gcgattcttg gcgctactaa cattaagcat atgtcattat tatcacatta tttaaaccac
     7261 attgatttga atatcaatga ggtggacatt atatacactg acaaatatga tatcgaagaa
     7321 catatccaag gcatcaataa ttactataaa tataaagtag atattaaaga agattggaca
     7381 tttatcaaaa aagctattgc ttactatcga tttaggccat acgctatgaa aattcttaaa
     7441 gaaaatcgtt atgattttgt catagtatgg ggaagttata caggacactt atttaaaagt
     7501 tttttagaaa aacactataa aaataaattc attttaaat
//
LOCUS       DBNENW010000121         7455 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNENW010000121 DBNENW010000000
VERSION     DBNENW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.122"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            56..1513
                     /locus_tag="AC1LW8_002084"
     CDS             56..1513
                     /locus_tag="AC1LW8_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIP4773891.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1506..2228
                     /locus_tag="AC1LW8_002085"
     CDS             1506..2228
                     /locus_tag="AC1LW8_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP4773892.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2379..3506
                     /gene="queG"
                     /locus_tag="AC1LW8_002086"
     CDS             2379..3506
                     /gene="queG"
                     /locus_tag="AC1LW8_002086"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIP4773893.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLASFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILGLAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3511..3981
                     /gene="trmL"
                     /locus_tag="AC1LW8_002087"
     CDS             3511..3981
                     /gene="trmL"
                     /locus_tag="AC1LW8_002087"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIP4773894.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4140..4739
                     /locus_tag="AC1LW8_002088"
     CDS             4140..4739
                     /locus_tag="AC1LW8_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIP4773895.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4764..4916
                     /locus_tag="AC1LW8_002089"
     CDS             4764..4916
                     /locus_tag="AC1LW8_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAS053 family DNA gyrase inhibitor"
                     /protein_id="HIP4773896.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5522..5917
                     /locus_tag="AC1LW8_002090"
     CDS             5522..5917
                     /locus_tag="AC1LW8_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773897.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6113..>7455
                     /gene="fumC"
                     /locus_tag="AC1LW8_002091"
     CDS             6113..>7455
                     /gene="fumC"
                     /locus_tag="AC1LW8_002091"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIP4773898.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQ"
BASE COUNT         2738 a          998 c         1410 g         2309 t
ORIGIN      
        1 atcaaaatgt attaatatgt ataattatga atattaatta gggagggctg tataaatgaa
       61 gtgtttaatt aggtttatat tagtattagg gttgttaatt agcagtgcta tggtgtatat
      121 caatcccaca gcacatgctg aacaagatca aacatgggag aagattaaag aacgcggtga
      181 acttagagtg ggtctttctg cagattatgc accaatggaa tttgagcata cagttaatgg
      241 taagactgag tatgcaggtg tagatattga tttagctaaa aaaattgcga aagataataa
      301 tttaaaatta aaaatcgtca atatgtcatt tgatagtttg ttaggagctc ttaaaactgg
      361 aaaaattgat attattattt ccggaatgac ttcaacgcct gaacgtaaga agcaagttga
      421 tttttcagat tcatatatga tgactaaaaa tatcatgctt gtaaagaaag ataaagttaa
      481 tgaatataaa gatatcaaag actttaataa taaaaaagta ggggcacaaa agggaactga
      541 acaagaaaaa atcgctcaaa ccgaaattga aaatgcatct attacttcat taagccgttt
      601 accagacgtt atattagcac ttaaaagtgg aaaggttgaa ggtgctgtag ttgaaaaacc
      661 tgtagcagaa gcatatttaa aacaaaatcc taaattagga atttccaatg tgaaatttaa
      721 tgaagaagaa aaagatacag tgatagcagt gccaaaagat tcaccaaaat tattgtcaca
      781 aattaataaa acgattaagg aggttaaaga taaaggatta atcgataaat atatgactaa
      841 tgctgcaaat gcgatgaatg atgacagtgg ttttatttct aagtatggta gttttttctt
      901 gaaaggaatt aagattacaa tattaatttc acttatcggt gttgcattag gttctatttt
      961 aggtgcattc gttgcgttaa tgaaattaag taaaataaaa attatttcat ggattgcttc
     1021 tatctatatc gaaatattaa gaggaacacc aatgttagtt caagtattta tcgtattctt
     1081 tggtataact gctgcattag gactagacat ttcagctctt gtttgtggaa caattgcatt
     1141 agttattaat tcctcagctt acattgctga aattattcgt gcaggtataa atgctgttga
     1201 taaaggccaa atggaagccg cacgtagtct aggtttgaat tatagacaaa cgatgaaaag
     1261 tgtaattatg ccacaagcaa ttaaaaatat tttaccagct ttaggtaatg aatttgtcac
     1321 tttaattaaa gaatcatcta ttgtgtcaac aattggagtt ggcgaaatta tgtttaatgc
     1381 acaagtggtt caaggtattt catttgaccc attcacacca ttaatagtgg cagcagcatt
     1441 atactttgtc ttaacatttg tacttacacg cattatgaac atgattgaag ggagattgaa
     1501 tgccagtgat taaaataaac aatcttaata aagtttttgg agataatgaa gttttaaaag
     1561 atatcaatct tgaaatcaat caaggggaag tagtagcaat aataggtcca tctggtagtg
     1621 gtaaaagtac attgttaaga tgtatgaatt tattagaagt acccactaaa ggtcaagtga
     1681 tttttgaagg caatgactta acggaaaaag ggacacaagt agataaacta cgtcaaaaaa
     1741 tgggtatggt atttcaaaac ttcaacctat ttccacataa aaaagttgtc gataatatta
     1801 ttttagctcc taaattatta aagaaagata ataacgatga attacataag gaagcattgt
     1861 cgttattaga taaagtggga ttaaaagaaa aagcagatgt atatccgaat caattatcag
     1921 gtggtcaaaa gcaaagggta gcaattgcaa gagctttagc aatgcatcca gatgttattt
     1981 tattcgatga accaacttca gcattagatc ctgaggtagt tggtgatgta ttaaaagtaa
     2041 tgaaagacct agccaaagaa ggtatgacca tggtggttgt gacacatgaa atgggatttg
     2101 ccaaagatgt aagtgacaaa gtcatattta tggcagatgg cgttgtcgta gagtcaggca
     2161 caccagtcga aatatttgaa caaccgcaac atgaaagaac acaaaatttc ttagcaagag
     2221 tattataaca acctaacgaa gcttgaatat atgatacgca ccacaaagtt atatcatata
     2281 ttcaagcttt ttattattaa aattatgtga aatgaaacct caatatgaat aatggttgtg
     2341 ttaaaataac atagtattat ttaatggggg gcatacgatt ggatacaaag cagttaaagc
     2401 aagatatcat tgactatgca tatacaattg gtatcgacag tattggattt acaactgccg
     2461 atccctttga tgaattgaag caaaagctag aagcatatca tgcaaatggt tatgcctcag
     2521 gatttgaaga atctgatatt gctttacgaa cggagcctaa attatcctta ccaacagcaa
     2581 ggtcaatcat agcaattgca gttggttatc ctaacaaact gaaaggtgca cctaagagtg
     2641 ttagaggtga tcgcagaggc ttatttgcta gagcatcgtg gggtcaagat tatcatacaa
     2701 ttatgcgtaa acgattagac atgttagctt catttattga atctaaagtt ccagatgttg
     2761 aaataaaatc tatggtagat acgggtgtat tatcagatag ggcagtagca gaacgtgcag
     2821 gtttaggatt tgttggtcgt aatggctttg tcatcaatcc taaactagga acatggacat
     2881 acctcggtga aatgttagtc agtatacctt ttgaacctga tgatccatta ttagatagct
     2941 gtggtgattg tacaatttgt gttgatcgtt gtccaacaag tgcattagtt ggaaatggcc
     3001 aattaaatag tcagaaatgc attagttttt taacgcaaac aaaaggctat atgcctgacc
     3061 aatatcgtta taaaattggg aatagattat atggttgcga tacgtgtcaa caagtttgtc
     3121 cgaaaaatag aggtattaat accgaacaag atgacatcat tttggaacca gaaattttaa
     3181 agccaagatt agtaccttta ctacgtatgt ctaataaaga attcaaacaa acatatggtc
     3241 accttgcagg tgcttggcgt ggtaaaaaac cgatacaacg aaatgctatt ttaggattgg
     3301 cgcattttaa tgaagtggat gcaattccag aattgaaaaa agttgcaaca acagatgaaa
     3361 gaccgatgat tcgagcaact gcttattggg ccattggtca aattcttggg gaagaagcaa
     3421 gagattttat caatgcaaat tatgatcaag aggatgcaga agttcaaaat gaaatgataa
     3481 aaggattaga cacaaggaga gaatagtaat atgacaaatc atatcgtttt atatcaacca
     3541 gaaatcccag ctaatactgg taacattgca cgtacgtgtg caggtacaaa cacacattta
     3601 catttaatta aacctcttgg atttagaact gatgataaaa tgttaaaaag agccggttta
     3661 gattattggg aatttgtgaa tattacgtat catgatagta ttgaagcatt ttttgaagcc
     3721 acaaatggcg agtattactt attaacaaaa tttggtaaaa agacttatag tgattttgat
     3781 ttttcaaatc atgacaaaga tttttacttc atttttggaa aagagacgac aggattacca
     3841 gactgggtga aagagaaata tcaagacaca gcattaagaa ttcctatgag tgaacatatt
     3901 cgttcattaa atttatcaaa tactgctgcg ttgttaattt atgaagcgtt acgtcaacaa
     3961 gattttccgg gattgaatta aaatttactc attttcatta taaaaatgat gcttaagtgc
     4021 tatatttaga gtataagcat gttaaagtta aatggtttga tatattgaaa gttagggtat
     4081 aataattatt aatatcgccg tttttatgta aaatgcaaac taagaggagt gtagaaatca
     4141 tggcaatgaa ctttaaagtc tttgacaata gtcaacttgt agcagaatat gctgctgata
     4201 ttattagaaa gcaatttaac aataatccta ctacaattgc aggttttcat ttagatacag
     4261 atcaagcgcc agttctagat gaattaaaga aaaatgttga aaaacatgct gttgatttta
     4321 gccaaataaa tattttagat tatgacgata aaaaatcata tttcgaagcg ttaggtgtac
     4381 cagcaggtca agtttatcca attgcttatg aaaaagatgc aatcgaatta atcgctgata
     4441 agattaaaac taaagaaaat aaagggaaat taacattaca agttgtttct atcgatgagc
     4501 aaggtaagtt aaatgttagt attcgtcaag gactaatgga agcaagagaa attttcttag
     4561 tagtgacagg tgctaataaa cgagatgtag ttgaaaaatt atatcaagaa aatggtaaaa
     4621 caagcttcga accagccgat ttaaaagcac atagaatggt aaatgttatt cttgataaag
     4681 aagcggctgc aggtttacct gaagatgtta aagcttactt tacgtcacgc tttgcttaat
     4741 taaaaatctg tgaggtattg attatgtcta aagacaaaga tccaaaatta aattatcatg
     4801 aagaagaaaa cagtatggta acggattttg aagatttaaa agaattaggt aaagaaatgg
     4861 aacaaatctc tgatcaaaat gatcaagaaa agaattctga agaagacagt cagtaaagta
     4921 ttataattgt gtaatattca gttatttatc atgtaaataa tgtatagtat agaacatata
     4981 tcaaatagga ttgattatct tgaatatgaa gttaggtagc attttgttac ctagcttttt
     5041 tattatattc atagaagctg actttctgcc agtttcagtg ttggggctgt ttccccaact
     5101 tacattgttt gtagaaattg aggatccaat ttttctatgt tgtggcccgt ttccccaatt
     5161 tgcaatgtct gtagaaattg ggaatccaat tccactatgt tggagctccg ccaacttgca
     5221 tgaaatagta agctgacttt ccaccagctt ctatgtttgt ggactgcagt taactactgc
     5281 caatataact ttgtaaagca taaaacattg atttgtgtac cagacatagt aatatatcaa
     5341 aagttcaatt ttaaatatta aaacaccatt taaacggaat tgataattaa ttactccaga
     5401 cataattgaa agaagtcatc tatcagtaat atttttaatg aaatcattaa accgcgtttc
     5461 ttaatatgcc aagattatga tatgctattg aagaatgaaa ttagaggaga tttatatatt
     5521 tatgttagga aaatactgga ttcaattatt gatagcaaca gttattataa gtttaatatc
     5581 aattaaagct tttccattgg caattggggc attatattta cctgttattt tcaaagtaat
     5641 aaaattacaa ctcaatttat caaaaggatt aatcgatgat gttaatgcgc aaacgttcat
     5701 taaaagtaat caatcaggta ttgtaataag tgtaatttgt tgtttattaa taacaggaat
     5761 attgtattat acacttgatg ggttttatgc gagtttaaca ggagttttag gtacgcttgt
     5821 tgctctaaat ccatatacga caattgttag tgctgtatta tatatactta cagctattgc
     5881 tacggttgaa gctactaaaa caaagtatcg taattaaact tagttcaaca ataactaaat
     5941 aatgatagat aacacactaa tcgtatgacc gtatcactgt aataaagggt gagtacgtgc
     6001 gattagtgta tttttatttc aaattatgaa aacgttttaa tcataaattg ctcaattaaa
     6061 ttatttaatg atatactaca gttaaataaa cagtgataag gggagaaatt gaatgtcagt
     6121 aagaattgaa catgatactt ttggagaaat agaagtacct gcagataaat attggggtgc
     6181 tcaaacagaa agaagtaaac gcaatttccc agttggtaaa gagcgtatgc caatcgaagt
     6241 agtttatggt tttgcacaac taaagcgtgc agcagcatta gctaattttg atttaggaaa
     6301 attaagcgag gcaaagaaag atgccattgt atacgcatgt gatcaaattt tatcaggtga
     6361 attagatgaa cacttcccac tagttgtatg gcaaacagga agcggtacac aaagtaatat
     6421 gaatgtgaac gaagtagtaa gttatgttgc taatatgtat ttaaaagatc atcaaattga
     6481 tgaaagtatc cacccaaatg atgatgtaaa taaatcacaa agttcgaatg atacattccc
     6541 aactgctatg cacgttgcat tatatcaaga ggttgaaaca aaattagaac ctgcattaaa
     6601 acttttaaga aatactttga aagaaaaaga agacaaattt gattcaatta ttaaaattgg
     6661 tcgtacacat ttacaagatg caacgccgat caaactagga caagagatta gtggctggcg
     6721 ttatatgctt gaccgttgcg aaataatgtt atctgaatct aagaagcaca ttttaaatct
     6781 tgccatcggt ggtacggctg ttggtactgg tattaatgcg catcctgaat ttggtgataa
     6841 agtggcacat tatatttcag aaaatacggg ttatccattt gtatcttctg aaaataaatt
     6901 ccatgcatta acagcacatg atgaagtagt acaactacat ggaacattga aggcgctagc
     6961 aggagactta atgaaaattg ctaatgatgt gagatggttg gcttcagggc cacgagctgg
     7021 tttggcagaa atttctatcc ctgaaaatga accaggttca tcaattatgc ctggtaaagt
     7081 taatcctaca caatgtgaaa tgttaacaat ggttgcagtc caagtaatgg gtaatgatac
     7141 agttgttggc ttcgcaagtt cacaaggtaa ctttgaattg aatgtttata aaccagttat
     7201 tatgcataat acactacaat caatttacct tttagctgat ggtatggaaa catttaataa
     7261 caattgcgca gtgggcattg aaccaatcga agagaatatt gataattatt taaatcaatc
     7321 attaatgtta gttactgcat taaatccaca tattggttat gaaaaagcag ctcaaattgc
     7381 taagaaagcc cataaagaag gtttaacttt aaaagaatct gcaattcaaa ctggatatgt
     7441 tacagaagaa caatt
//
LOCUS       DBNENW010000122         7358 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNENW010000122 DBNENW010000000
VERSION     DBNENW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.123"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(32..394)
                     /locus_tag="AC1LW8_002092"
     CDS             complement(32..394)
                     /locus_tag="AC1LW8_002092"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIP4773899.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(391..561)
                     /gene="mazE"
                     /locus_tag="AC1LW8_002093"
     CDS             complement(391..561)
                     /gene="mazE"
                     /locus_tag="AC1LW8_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HIP4773900.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(646..1794)
                     /gene="alr"
                     /locus_tag="AC1LW8_002094"
     CDS             complement(646..1794)
                     /gene="alr"
                     /locus_tag="AC1LW8_002094"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIP4773901.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(1860..2219)
                     /gene="acpS"
                     /locus_tag="AC1LW8_002095"
     CDS             complement(1860..2219)
                     /gene="acpS"
                     /locus_tag="AC1LW8_002095"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIP4773902.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(2223..2714)
                     /locus_tag="AC1LW8_002096"
     CDS             complement(2223..2714)
                     /locus_tag="AC1LW8_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP4773903.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(2701..4284)
                     /locus_tag="AC1LW8_002097"
     CDS             complement(2701..4284)
                     /locus_tag="AC1LW8_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP4773904.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(4277..4756)
                     /locus_tag="AC1LW8_002098"
     CDS             complement(4277..4756)
                     /locus_tag="AC1LW8_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP4773905.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLCIKPLWQYHCRFYKVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(4965..5525)
                     /locus_tag="AC1LW8_002099"
     CDS             complement(4965..5525)
                     /locus_tag="AC1LW8_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HIP4773906.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(5545..>7358)
                     /gene="kdpB"
                     /locus_tag="AC1LW8_002100"
     CDS             complement(5545..>7358)
                     /gene="kdpB"
                     /locus_tag="AC1LW8_002100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIP4773907.1"
                     /translation="LITILFANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYR
                     IVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVI
                     GGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVV
                     IVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGR
                     AVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKS
                     IVRLAKQMYINELPKDIDGTYKTFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGN
                     IPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVM
                     CTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALA
                     QANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVA
                     KYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKG
                     YSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
BASE COUNT         2302 a         1460 c         1025 g         2571 t
ORIGIN      
        1 tttatctctg aaaaatacat ataatagacg cctaattttt ctggtgagct actgcattca
       61 gccctaaact aatcattagt gcattatcta cttctttcat tttatcatcg gataagtacg
      121 tcagtttttc tttcaatcgt tttttatcaa gtgtacgaat ttgttctaat aatataactg
      181 agtctttatc caacttatac tttttctttt caatctctac atgtgtcggt attttcgctt
      241 tattaatcct accagttatt gccgcaacaa taactgtagg actatattta ttaccagtat
      301 cattttgaat tatgactaca ggtctgactc ccccttgttc agatccctgt actggtgata
      361 aatctgctaa ataaacatct cctcgtctaa tcattcattc gttgaattag aagataaata
      421 tgtttcgttg caatcgcatg cttcacactc tatcggaaaa gcttcgttcg ctagggagag
      481 atttaaatca gccatttgtg aatatccttc ttttaaagat tgttctaagc tatgacttct
      541 attttgacta aaagataaca tatgagaacc tccaattgat tccaaaagtt attaaatgac
      601 ctttaattac tctaatgata acaaatttta tatgcactga ccatattatt ttaacaattc
      661 atttgttaca aaacgttgat caccatcatg atagattcgc ggcaaacgtc tcgacaagtt
      721 acaaagcact tcataattaa tagtatgttg cttttcagct accacctcta ccgactgtgg
      781 actttctcta tgattatcta ttaaaatcac cgaatctcca gctttaactt gatctggcac
      841 tttaacaatt gtctgatcca tacatacgcg accaataact tcgcattgat gaccatttac
      901 atttacaaag ctaccttgca ttatgcgtaa atagccatct gcatatccaa taggtaacaa
      961 tgctattgta gttgggtcag tagctgtata agttgcacca taacttacag actcacccgc
     1021 ttgtagcgtc tttgtttgaa ctacattagc aattaattgc acacttggtt taaggtgtac
     1081 tttaactttt tgctgtacat actctgatgg ataatatcca taaagggaaa ttcctggtct
     1141 tattgcatta cagaattggc aatccattag tagagaacct gctgagttct gacaatgtat
     1201 atattcaggt ttaattgctt cattgaccat atctttaaaa cgttgatatt gttcagttgt
     1261 catatctcct ggttcgtcag cacaggcaaa gtgtgtaaac acgccttcaa atacaagttg
     1321 ctcatattgt tgaatgattt caatcacttc ttgatacgta ttagtatctt taatacctaa
     1381 acgtcccatt cctgtatcta atttaatgtg caaccataac tttttctctt gctcaccaga
     1441 aatgttttta attgcttctt tcaaccactg tttagacgga accgttaagg caactcggtg
     1501 ttgtatcgct ttatcaatat ctttagctgg taacacacct aagactaaaa ttttagcagt
     1561 aatcccatgc attctaagtt ctatcgcttc atctaacgtt gctacagcaa aaaatgtggc
     1621 gccattttcc attaaatgac gtgctacttt aacactacct agtccatagg cattggcttt
     1681 aacgacagcc atcactgttt tatttggatg caatgtactg aatactttga aatttgatgc
     1741 aacagcgttt aaatctacat tcatatacgc agatctataa tatttatccg acatattact
     1801 tcctcctgta attcccacac gttttaaaac tagatcttaa ttatcattgt ataacaaatt
     1861 taaaatgctg acttttctaa aacaacttgg ctcatcgcat aatgctcagt gtgtgagata
     1921 ctaacatgta cgatgaaccc ttcgtaatca atctttggtt tgccaagttc gtcgttgtaa
     1981 cagtctatat cgttaaaagc tacatgtttt cctaagcctg tgcctaatgc tttactgaac
     2041 gcttcttttg tagcaaacct gccagctaaa aattcaattt tacgttgctc atgtgtgaaa
     2101 ttgttgaatt tgtgctgttc atttttagtt aaaatccgct caaccaattt tggctgctta
     2161 ctatataaca cttttattcg atcgatttcg attaaatcta caccaattcc atgtatcatt
     2221 gattattcct catttttatt gttttctaaa tgtctctcgt tatttaacca tcgtttcaat
     2281 ctacgtgctt cacttctatc tattaattca atttcagctt gaccacccgc tgtataaagg
     2341 gtcaaagttg ctaatcgata gcgtctcatt ataggaccaa catcgatatc aatattttga
     2401 atacgaaaat atggtattac tttttcatcc aaaaataaaa tgccgtttcg tacacgcaaa
     2461 tggtgttttt caaatgcata tctgcagtgc ttatatcgat atactggcgc tatgacaagc
     2521 gtgaaaacag caacaagtaa tattataatc acactactaa caatggatat attgttatct
     2581 aatatctccc aaaatagcca gttcaaaata ttaaatgcga ttaaaagtac aagcgctatg
     2641 ggtatccaaa acagcacagc acttaacctc atcacttttt tagcgtgtgg tgacataaaa
     2701 ttataatccc tcatcatttt cacctcttaa ataccatgat ttcaatttat ttgcatcttc
     2761 acttcttgcg tattttaggt taatcgattg ggctcccaca cctttagcaa caataaagct
     2821 aaaattattt aaattgttgc gtttaagtaa tgtatgttgc caagtgtcaa atcctataat
     2881 gtgatgcgct ttaaaataat aaatatttcg tttcaatagc tcgaaattct ggatagtaat
     2941 ttgttcttct gtcattttaa aacccgcatg tttgacataa agatatcctt tgatcacaaa
     3001 taaaccaata atgactattg ttataatcgt aaataacaat aataattgat tccaaaaata
     3061 acagccaata cctgccatag ctatgacaat aatactaggt attaaaaagt gtctgtggaa
     3121 acctgacaaa ggcatacctt cattaacttg ttgataagat aaatctggta ctaaattctg
     3181 gataatttga tatgctttgt ctcgtttaat aaacggcaat atcggcacac tacctgaatc
     3241 attgtcatca tcttcgattt ttttattttc aaaatcactt gtgatgataa aatgtattgc
     3301 tgtgtagcca aataatcttc gtatatatga ctgacgttca acaacagctt gcaatcgttt
     3361 cgttggtaca gttactttct tcttgttaaa gagaccatat tcaattttaa gttgctcatt
     3421 ttcaagcatc accgaaaagc cataaaatct tatcatcgtt ataatcgttc caataatata
     3481 tgccactatt aatactagta aaatgatgat taatactgaa atacttacaa tttgaaccca
     3541 ttgactaatt tcatgattta gcttcgacca tgggatcaac tctcttacag ccccgtaaat
     3601 cggtactaaa gctgctaacg ttacaccaat ggcgccactg gtcattgcca taaatagtga
     3661 ttctttaaaa ttcatctgat atataggaat gcgtttattt ttctgattaa gcatactatc
     3721 agtgttctgc acttcatcta agcgaccttc tgcgatgtct tccacattac cttcaatgtc
     3781 atgattacag ttgtcattct tctcagcact agacttttgc gccacttctg tcttcaactc
     3841 tgtttgcaat tgatcaatat agcgttcaag atattcacct tgttttttcg aaataacact
     3901 taagacaata ccatcacttg gtgttttgat ttgtaaatcc acaccaccaa taatttgatt
     3961 gacgacccct tgtgtcatat caacggactg aatacgtttt atatttaatt ctttacgctt
     4021 tttattaaaa atacctgttg ttaaaatgaa ataattatcc tcaatccaat atcgcgtgtt
     4081 cataattccg acaatttgag aaatgtatga tattaaaaag aatacaaata caatacctat
     4141 ccataaatat gattcgggat tcgtataatc aaaatctttc aattgaaaga taatgaaaat
     4201 aaaaaagacg actatgtttt gtttgatagc attgattatg ccattaaaat atgaaatcgg
     4261 atgtaatttt tgaggttcag acatcacttt caacccctct caaattcgac atagttctcg
     4321 cttcgattat tttaacatcg tcatgagaca tcatcggtaa ataaatagta tgacctgcag
     4381 tcataaatcc aactttatac aaattaagca ctttactaat tggattagat ttaatcgaca
     4441 agtattgtaa acgttcaatt cgactcgttt cttctttata tattaaaaat gatgtacgat
     4501 attgtacact tagttgatca actttataaa agcgacaatg atattgccat aaaggcttaa
     4561 tacataattt taatgtactc agagcaccta aaaccaacaa aatataaagt aagtaatgtg
     4621 gccattcaaa tcttaaccat ataaaataaa aaatgacata cacagctaca ctcaatataa
     4681 attctaagcc attcgtaatg taataataca acaatgctga cttaggactc ttagacaact
     4741 tagtataatc tgacatatac ccctatcccc aaataaaaaa ttatacggat ttataatcta
     4801 tttcatttta tttttatatg ttgctaatta tagcatatgg aatatttcat gctaatttat
     4861 tcttcctaaa ggtacatcta aaaatttagt taagcagaaa gtgcttgaat cgctaaaaag
     4921 acaccatgtt ataattttat caacatgatg cctttcatct ataatcaatc tttcatctta
     4981 tcaagagcaa tatttagttc aagcacattc acataatcat ttgttaacac accacgctgc
     5041 ttacgatgtt gaatcaagtc ggccactctt gaagtagata tatgacgagc atcagcaata
     5101 cgaggtgctt gcttcaatgc attttcaacc gtaatatgcg gatctaagcc cgacccagaa
     5161 cttgttgcag catctattgt tacatttgaa ttcccaaatt taacatgatg tttcatgcgt
     5221 gctattaatt cggtgtttcc attcgattca ttacttccac ctgatgatac gccgttttta
     5281 tataattttt caggattcat attataatca actgcactcg gtctcccgtg aaaatatcgt
     5341 gtctctgtcc agtgctgtcc aatcaatttt gatcctacta tacgattgtc atacgtaatt
     5401 aaactgccgt ttgcttgttg ataaaaaaat atttgaccaa ttaacgtgat agctaacggg
     5461 aataaaaatc cacataatac catagttatt atcgttaaac aaatactatt tcttatcgta
     5521 ttcatggtac aggctccttc ctctttacac aaaaaattgt acaatcatat ctattaattt
     5581 aatgcctaaa aacgggacga ttaatccacc taatccataa atcaacatat tatttataaa
     5641 aattctatca atgctgtaac cctttacttt tacacctttc atagcaattg gaattaaggc
     5701 aacaatgatt aatgcattga atatcaaagc tgatataatt gcagatttag gtgacgataa
     5761 atgcataata tttagtgaag tcatttctgg aattgttgac atcattagtg caggtaaaat
     5821 tgcaaagtat tttgctacgt cattagccaa actaaatgtc gttaatgcac ctctcgtcat
     5881 taataattgt ttgcctattt ttacaacctc tattaacttt gtaggattcg aatctaaatc
     5941 aattagatta gctgcctctt tagcactaat tgtccctgag ttcatagcta atcctatatt
     6001 cgcttgtgct agcgcaggtg catcatttgt accatctcct gtcatcgcaa caatatggcc
     6061 tttcgcttgt tcatctttga tgactttaat tttatcttcg ggtttacact ctgcaacaaa
     6121 tctatcaacc ccggcttctt ttgcaattgt agctgctgtt aaagcattat cacctgtaca
     6181 cataactgtt tcaatcccca tttttctcaa ttcagtaaat cgttctacaa gaccatcttt
     6241 aatcacatct tttaaataaa tcacgccaag catgacattg ttttcaatga ctattaatgg
     6301 tgtgccacct ttactcgata catccataca gagagactca atattaagag gaatattgcc
     6361 ttgttgttgt ttgacaagat ttatcatact attaggtgca cctttgaata ccgatatttc
     6421 atttgtaatg attccgctca ttctagtttc agctgtaaaa gtcttatatg tgccatcaat
     6481 gtctttaggc agctcattta tatacatctg cttcgctaat cgtacaatac tttttccttc
     6541 tggcgtatca tcgtagattg atgacatata agcagcgact atcaattttt ccagcatttg
     6601 ttgattcact ggtaaaaatt cactagcgat tcgattgcca taagtgattg tgcctgtctt
     6661 gtctaaaatc attacatcga catctccaca tacttctaca gcacgcccac ttttcgctaa
     6721 tacattgaat tgagtaacac gatccatgcc tgcaatacca atcgccgata acaaaccacc
     6781 gattgtcgtt ggtattaaac atactgttaa cgcaatgagc atcgcaatag gtaaaattaa
     6841 atgcaggtaa gatgctattg gatataacgt tacaataacg actaaaaata taattgttaa
     6901 cgttgttaat aatgtaaaaa gtgcaatttc atttggtgtt ttatttcttt ccgccccttc
     6961 aactaaggca atcattttat ctaaaaaaga tgtacccgct tcactctcaa cacgtatttc
     7021 taaccaatca gatgttacaa gtgtaccgcc aatgactcca tcaaaatcgc cacctgattc
     7081 ttttatcaca ggtgcagact caccagtaat tgcagattca tcaacggttg ctaatccatt
     7141 tattacaacg ccatcagcag ggattgtttc tccattttct acccgaatat tttgtccggc
     7201 ttttaactct gtggcgttca ctatccgata cgcaccattt tcttctatca atcgagcagt
     7261 taaatttgat tgtgcttgtc ttaaactatc agcttgcgct tttccacgac cttcagcaaa
     7321 ggcttctgaa aaattagcaa acaatatagt tattaata
//
LOCUS       DBNENW010000123         7283 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNENW010000123 DBNENW010000000
VERSION     DBNENW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.124"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            90..204
                     /locus_tag="AC1LW8_002101"
                     /pseudo
     CDS             90..204
                     /locus_tag="AC1LW8_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            484..1143
                     /gene="arlR"
                     /locus_tag="AC1LW8_002102"
     CDS             484..1143
                     /gene="arlR"
                     /locus_tag="AC1LW8_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HIP4773908.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1140..2495
                     /gene="arlS"
                     /locus_tag="AC1LW8_002103"
     CDS             1140..2495
                     /gene="arlS"
                     /locus_tag="AC1LW8_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HIP4773909.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2779..5577
                     /locus_tag="AC1LW8_002104"
     CDS             2779..5577
                     /locus_tag="AC1LW8_002104"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIP4773910.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5591..6859
                     /gene="sucB"
                     /locus_tag="AC1LW8_002105"
     CDS             5591..6859
                     /gene="sucB"
                     /locus_tag="AC1LW8_002105"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIP4773911.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2758 a         1050 c         1305 g         2170 t
ORIGIN      
        1 tatttctcat tagaattaga atatttaaaa gttatgaggt ttaaaaatta tattgtttac
       61 atactccttt tgaatttata cattatgaaa tgttacttcc aagttcaaaa tcgcacattg
      121 aaatgatgtg tgaaaatgtt taaactacgg tcattttgtg aaaataaaga taataactat
      181 tcattttaca ttagtgaaaa gtaagtatac gacaacaatt gatattgcgg taaggccttg
      241 tgttacagta ttctatattt aagtactgca atcagaatta acagaattcc attaactgat
      301 tattaaatat ttgagttaat aaataattaa tgattgtagc ttgaaaaatt taaaacatgg
      361 ttattgattt gtgataaaat ttaaacgtaa acaaactaat ttaaaaagca actattgtat
      421 agaaaaatac aaaatttaaa atatattacc ttattagaaa aagtcgtaat atgaggtgta
      481 caaatgacgc aaattttaat agtagaagat gaacaaaact tagcaagatt tcttgaattg
      541 gaactcacac atgaaaatta caatgtggac acagagtatg atggacaaga cggtttagat
      601 aaagcgctta gccattacta tgatttaatc atattagatt taatgttgcc gtcaattaat
      661 ggcttagaaa tttgtcgcaa aattagacaa caacaatcta cacctatcat tataattaca
      721 gcgaaaagtg atacgtatga caaagttgct gggcttgatt acggtgcaga cgattatata
      781 gttaagccat ttgatattga agaactttta gcaagaatac gtgcgatttt acgtcgtcag
      841 ccacaaaaag atattatcga tgtcaatggt attacaattg ataagaatgc ttttaaagtg
      901 acggtaaatg gcgcagaaat tgaattaaca aaaacagagt atgatttact atatcttctt
      961 gctgaaaata aaaaccatgt tatgcaacgg gaacaaattt taaatcatgt atggggttat
     1021 aatagtgaag tagaaacaaa tgtcgtagat gtttatataa gatatttacg aaacaaatta
     1081 aaaccatacg atcgtgacaa aatgattgaa acagttcgtg gcgttgggta tgtgatacga
     1141 tgacaaaacg taaattgcgc aataactgga ttattgttac cacgatgatt acgtttgtca
     1201 cgatattttt gttttgttta attattattt ttttcttgaa agatacactg cataatagtg
     1261 agcttgatga tgcagaacga agctcaagcg atattaataa tttatttcat tctaagcctg
     1321 ttaaagatat atctgcatta gacttgaatg catctttagg taattttcaa gagataatta
     1381 tttatgatga gcataataat aaattatttg agacatcgaa tgataacaca gtgagagttg
     1441 aaccaggtta tgaacaccgt tattttgacc gcgtaataaa aaaacgctat aaaggcattg
     1501 aatatttaat tattaaagaa ccaattacaa cgcaagattt caaagggtat agcttgttaa
     1561 ttcattcact agaaaattat gataacatcg taaaatcatt gtatatcatt gcgctggcat
     1621 ttggagtgat tgcaacaatt ataactgcca caatcagtta tgtattttca acacaaatta
     1681 ctaaaccgct tgtcagttta tcaaataaaa tgattgagat tcgacgagat ggttttcaaa
     1741 ataaattgca attaaataca aattatgaag aaatagataa tttagcaaat acgtttaatg
     1801 agatgatgag ccaaattgaa gaatcattta atcaacaaag acaatttgtt gaagatgcgt
     1861 cacatgaatt acgaacacca ttacaaatta ttcaaggtca tttaaatttg attcagcgat
     1921 ggggaaaaaa agacccagca gtattagaag aatcgttaaa tatttctatt gaagaaatga
     1981 atcgtatcat aaaattagtc gaagaattac ttgaattgac taaaggagat gtaaatgaca
     2041 tttcttctga agcacagacc gtgcatatta atgatgaaat tcgctcgcga atacactcat
     2101 taaaacaatt gcatcctgat tatcaatttg atacggatct gacatctaaa aatctagaaa
     2161 ttaaaatgaa acctcatcaa ttcgaacaat tatttttaat ctttattgat aatgcaatca
     2221 aatatgatgt gaagaataag aaaattaaag ttaagacaag gttaaaaaat aagcaaaaaa
     2281 taattgaaat tacagatcat ggaattggta ttccagagga agatcaagat ttcatttttg
     2341 atcgctttta tcgagtggat aaatctcgtt caagaagtca aggcggtaat ggactcggat
     2401 tatctattgc tcaaaaaatc attcaattaa acggaggatc gattaaaatt aaaagtgaaa
     2461 ttaacaaagg aacaacgttt aaaatcatat tttaatcatg actgagacgt caatcaaagt
     2521 cataggatca attttttaag tacacattag ctgtgactaa tgtataagaa caactataaa
     2581 acaaataaac agtggttctt tatcatttct gttgtactcc caaaatttac aataaaatac
     2641 atctataaac ctagaagaat caacgctttt gttgattctt ctttttagca gataaatagg
     2701 taaatctact ttaacaaata actaaatagt gatattatta cattgtaagc gtttcaacat
     2761 ttttgtggag ggtgtaaaat gactaacgaa agaaaagaag tttcagaggc tcctgtaaac
     2821 ttcggtgcga atttaggtct aatgttagat ctatatgatg actttttaca agatccatca
     2881 tctgtaccag aagatttaca agtcttattc agcacaatta agaatgatga ctcaattgta
     2941 ccagctttaa aaagtacaag tagtcaaaat agcgacggca caattaagcg tgtcatgcgt
     3001 ttaattgata atattcgcca atacgggcat cttaaagccg atatttatcc tgtaaatcct
     3061 ccaaaaagga aacatgtacc taaattagag attgaagact ttgatttaga tcaacagact
     3121 ttggaaggta tatcagcagg aattgtttca gatcactttg ccgacattta tgataatgct
     3181 tatgaagcaa ttttaagaat ggaaaaacgt tacaaaggac caattgcatt tgagtataca
     3241 catattaata acaataccga acgtggttgg ttaaaaagaa gaattgaaac gccatataaa
     3301 gtaacgttaa ataataacga aaaaagggca ctattcaaac aattagcgta tgttgaaggg
     3361 tttgaaaaat atcttcataa aaacttcgtt ggtgcaaagc gtttttcaat tgaaggggta
     3421 gacgcacttg taccgatgtt acaacgtact attacgattg ctgcgaaaga aggtattaaa
     3481 aatatacaaa taggcatggc tcaccgtgga cgtttaaacg ttttaacgca tgtcttagaa
     3541 aaaccgtacg aaatgatgat ttcagaattt atgcatacag atccaatgaa attcttacct
     3601 gaagatggta gcttgcagtt aactgctgga tggactggtg atgtgaaata tcaccttggt
     3661 ggcattaaaa ctactgattc atacggtaca atgcagcgta ttgcactggc taacaatcca
     3721 agtcacttgg aaattgttgc acctgttgtt gaggggcgta cgagagcagc acaagatgat
     3781 acacaacgag ctggggctcc gacgactgat catcataaag caatgccaat tattatacat
     3841 ggcgatgctg cttatcctgg tcaaggaatt aacttcgaaa caatgaactt aggaaacttg
     3901 aaaggctatt ctacgggtgg ttcattgcat attattacta acaatagaat tggatttact
     3961 acagaaccaa ttgatgcacg ttcaacaact tattctacag atgtggccaa aggttatgat
     4021 gtgccaatat tccatgtcaa tgcagatgac gttgaagcta ctattgaagc aattgatatt
     4081 gcaatggaat ttagaaaaga gtttcataaa gacgtcgtta ttgatttagt aggttatcgt
     4141 cgtttcggac ataacgaaat ggatgaacca tcaattacta atccagttcc ttatcagaat
     4201 attcgcaaac atgactctgt tgaatatgtg tttggtaaaa agcttgttaa tgaaggtgtc
     4261 atttcagaag atgaaatgca ttcatttata gaacaagtcc aaaaggaact aagacaagct
     4321 catgataaaa ttaataaagc tgataaaatg gataatccag atatggaaaa gcctgcagat
     4381 cttgcattac cgttacaagc agacgaacaa tcatttactt ttgatcactt gaaagaaata
     4441 aatgatgcat tgttaacata tccggatggc tttaacattt tgaaaaagtt aaacaaagtt
     4501 cttgagaagc gtcatgagcc gtttaataaa gaagatggtt tagttgattg ggcacaagca
     4561 gaacaacttg catttgcgac aattttacaa gatggtacac cgattcgctt aactggtcaa
     4621 gatagtgaac gtggtacatt cagtcatagg catgccgtgt tacatgatga gcaaacaggt
     4681 gaaacatata cacctttaca tcatgttcct gatcaaaaag cgacatttga tatacacaat
     4741 tctccgcttt cagaagcagc agtagttggt tttgaatacg gctataatgt ggaaaacaaa
     4801 aaaagcttca atatttggga agcacaatat ggtgattttg caaatatgtc acaaatgatt
     4861 tttgacaact tcttattcag ttctcgctca aaatggggag aacgttcagg attaacatta
     4921 ttcttacctc atgcatatga gggtcaaggg cctgaacatt catcagcaag attagagcga
     4981 tttttacaat tagctgctga aaataattgc acagttgtca acttatctag ttcaagtaat
     5041 tatttccact tattgcgtgc acaagcggct agtttagatt ctgaacaaat gcgaccattg
     5101 gttgttatgt caccaaaaag cttactgaga aataaaacag ttgcaaaacc aattgatgaa
     5161 tttacttctg gtggatttga gccaattttg acagaatcat atcaagcgga taaggttaca
     5221 aaagttattt tggcaactgg taaaatgttc attgatttaa aagaagcatt agctaaaaat
     5281 ccagacgaat cagtattact cgttgcgatt gaaagattgt atccattccc agaggaagag
     5341 attgaagcat tactagcaca attgccaaac cttgaagaag tgtcatgggt acaagaagaa
     5401 cctaaaaatc aaggtgcctg gttatatgtc tatccatatg ttaaagtgct agttgcagat
     5461 aaatatgatt taagttatca tggcagaatt caaagggctg ctccagctga aggcgatgga
     5521 gaaattcata aacttgttca aaataaaatt atagaaaatg cattaaaaaa taactagggg
     5581 gaaataagtc atgccagagg ttaaagttcc agaattagca gaatctatta cagaaggtac
     5641 cattgcagaa tggttgaaaa acgtagggga tagcgtagaa aaaggtgaag ctattcttga
     5701 attagaaact gataaagtta atgtcgaagt tgtatctgaa gaagcaggtg tattatctga
     5761 acaacttgca agtgaaggcg acactgtaga agttggacaa gcaattgcta tcatcggcga
     5821 aggtagtggc aatgcttcta aagaaaatag taacgacaat actccacaac aaaatgaaga
     5881 aacaaataat aaaaaagaag aaacaacaaa taattcggta gataaagctg aagtaaatca
     5941 agcaaatgat gacaatcagc aacgtattaa tgctacgcct tctgcgcgtc gatatgctcg
     6001 tgaaaatggt gtgaatcttg ctgaagtaag tccgaaaaca aatgatgtgg ttcgtaaaga
     6061 agatattgat aagaaacaac aggcaccggc atcaacacaa acaacacaac aagcatctgc
     6121 aaaagaagag aaaaaataca atcaatatcc tacaaaacca gtgattcgtg aaaaaatgtc
     6181 acgtagaaag aaaacagctg ccaaaaaatt attagaggta tctaataata cagctatgtt
     6241 aacaacattt aacgaagttg acatgacaaa tgttatggaa ttgcgtaaac gtaagaaaga
     6301 acaatttatg aaagatcatg atggtactaa attaggattt atgtcattct ttactaaagc
     6361 ttctgtagca gctttgaaaa agtatccaga agttaatgca gaaatcgacg gcgacgacat
     6421 gattacgaaa caatattatg atattggtgt agctgtttct acagatgatg gattattagt
     6481 accatttgta agagattgtg ataaaaagaa ttttgcagaa atcgaagcag aaattgctaa
     6541 tttagcagtt aaagcacgag agaaaaaact tggcttagat gatatggtta atggttcatt
     6601 tacgattaca aatggcggta tttttggatc aatgatgagt acgccaatta tcaatggtaa
     6661 tcaagctgca atcttaggca tgcattcaat tattacaaga ccaattgcga ttgatcaaga
     6721 tacaatcgaa aatcgtccaa tgatgtatat tgcattaagc tatgatcata gaattattga
     6781 cggtaaagaa gcagttggat tcttaaaaac aattaaagaa ttaattgaaa acccagaaga
     6841 cttattatta gaatcttaat ccacaacaca aaatagttta ctattcctaa aagcgggatt
     6901 aaatcaataa caaacagcag taagattatt tcctagtcga aatatcttac tgctgtactt
     6961 tatttttata atgattcgta ttaaaatgtg ggtttaaatt tgattactta aaaaatatat
     7021 catttctatt gaaatagact cacaatagaa atataggtag aatagcgtgt tacaatatgc
     7081 taatgaaatc attttagata ataacaagcg atgaaattta tgaattctat aagcgccttt
     7141 agttaattta aaatcatctg aacaagatgt aaattcactt tatgaacacg acatatatat
     7201 gttttttatg tactacaaaa aacgaaatat tcaattgcta gatacagata ctcattaatg
     7261 acagactcat tagcaaccat aac
//
LOCUS       DBNENW010000124         7193 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNENW010000124 DBNENW010000000
VERSION     DBNENW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.125"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(1298..1681)
                     /locus_tag="AC1LW8_002106"
     CDS             complement(1298..1681)
                     /locus_tag="AC1LW8_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HIP4773912.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            1864..3306
                     /locus_tag="AC1LW8_002107"
     CDS             1864..3306
                     /locus_tag="AC1LW8_002107"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HIP4773913.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            complement(3391..4335)
                     /locus_tag="AC1LW8_002108"
     CDS             complement(3391..4335)
                     /locus_tag="AC1LW8_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIP4773914.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(4381..5004)
                     /locus_tag="AC1LW8_002109"
     CDS             complement(4381..5004)
                     /locus_tag="AC1LW8_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP4773915.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            5165..6442
                     /locus_tag="AC1LW8_002110"
     CDS             5165..6442
                     /locus_tag="AC1LW8_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HIP4773916.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            6649..7080
                     /locus_tag="AC1LW8_002111"
     CDS             6649..7080
                     /locus_tag="AC1LW8_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HIP4773917.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
BASE COUNT         2506 a         1051 c         1182 g         2454 t
ORIGIN      
        1 aaaattagaa agatagtaag caaaatttgg aaattttcga tagagtctaa ttaaattatt
       61 tggtgcataa ttaatataga tttcaaacct atgtatttca aaaaacaata catagccatt
      121 ttgaactaac ttgctaatct tttattgata ttcaactatg ttataaaaaa agcaaattga
      181 ccaaaatgga agtttgaatc aaaaaacatt aaatgtaccc atgacttaaa ttttatcgcc
      241 attatttaag tttggacata cacttttgtc atcaaacgct gaaaattagt acgattgaaa
      301 tcacttcttg ttataaaaat tcttagtagc cctgttacaa aatattcgct acaaattatt
      361 caaatcaaca acaataacca tcaactaaga aattttaaac aatctactaa aaaataaagc
      421 gtttaaaagt tctgtttaga aaacatcaag ataagtataa acatctagaa aagtgaacat
      481 ctaagaaaat ccatctagtt attgccgtaa caattagaat aatcatttga aaaagtgcat
      541 tagacaacga aattcaccat caagataatg aagcaacaag aaacagcaca catcaatgaa
      601 ttaacacctt agattcatca tgagaaaatg catggcacaa ctagatttac catcaagata
      661 attcttaagc agcgaagaat taacatctag aaatgcataa acatcttaga ttttacatca
      721 agagatgcga taactataaa caactagaaa cgtcaccaag atagtgcaac aacaaagaat
      781 cagcatcttg aaatcgcact aacatctaag aatcaacatc aagtattata ttaacatcat
      841 agataacttc atcaagacag gcatccacat ctaagaaact catctagaat agctaacacc
      901 taacatcaaa gaatattcat caagaacagt atctaacaga actttttgac tatatatgaa
      961 ttaattatgt tactagctaa atcatctatt attaactttc acactcaatt tctacatcta
     1021 atatcaattt attaacaata gaattagcag caacaagata ttacttaaaa ttactccccc
     1081 gaatttaaaa tataaacaat gtaataatta agggtataaa ttgatttatg ctgtaactaa
     1141 ttaaatgtgt atggtatctt ctaacatcat gaaacgcatt ttaatataga tatgtacatt
     1201 taaaccttga gagatagatt atagtaaact ttagtacaaa gtatgaattg ctgaactcca
     1261 atgactacta tttgtccgtt acaactaaag tttcaatcta tctctttctt tttgtgttta
     1321 aaacacgact ttgtagtaaa tattgaacat catatttatt agcaagttct gctaaaaaat
     1381 ctaataaagt ttgggtgtcg attttaccat taataaaatc agctttaagt agttttgatt
     1441 gttgtctttc ttcttcattc gcacattttt taaaatatcc ccacatatga tcaaaagcat
     1501 tacatacact tcctatactc ggttcaatta acaaagcatc atcaatcatt tgttgaactt
     1561 gatgaagtga taaatccgtt tttaaagcat tccttatcat ttggtaagac tgttgactgt
     1621 gtaacaatac atgatatttt tcctcacgcc aaagctgttc gatatagcca cgttctttca
     1681 taaaattacc tccgataatt aatgttaata tacatgcatg tagtatagca ataaaaatga
     1741 atgcgctatc aaaaatggtg taggtgttta tataaaatga taatagagtg ttgcaaatat
     1801 ttttggaacc ggttacaata aagtggaata aatggaaccg gttctaatta ggaggctaaa
     1861 tatatgaatt ataagcaatc cgcagaagaa attttgaacg cgataggcgg agaagagaat
     1921 ttagatgcaa tggcacattg tgcaacgaga ctacgattag ttttaaatga tgaaagttta
     1981 gtaaatgaag aggcgctaaa caatatggat gtagttaaag ggacgttttc tactggggga
     2041 caataccaaa ttattattgg gtctggtaca gtcaataaag tatttagtga actggaaaaa
     2101 ttaactggaa aagaagcatc aaccacttcg gaagtcaaag cacaatctgc taaaaatatg
     2161 aatccgttac agcgatttgt aaaaatgctt tcagatatct ttgttccgat tataccagcc
     2221 atcgttgctg gtggtttatt aatggggtta aataacattt tgactgcgaa agatttattc
     2281 ttttcaggta aatcattgat agatgtatat agtcaatttg ctggattagc tgaaatgata
     2341 aatgtttttg cgaatgcacc atttacatta ttaccaattt taattggatt tagtgcagca
     2401 aaacgctttg gtggcaatcc atttttaggt gctgcattag gtatgatact agttcatcca
     2461 tcgctaatga gcgcatacga tttcccaaaa gcagttgaag caggtaaggc tattccatat
     2521 tgggatgttt ttggtttgca tattaatcaa gtaggttatc aaggacaagt gttacctatg
     2581 cttgtagcag cttatatctt agcctcaatt gaaaaagggt tacgcaaagt tattccaacg
     2641 gtgttagata atttgttaac accattgtta tctattttta taacagcatt tctaacattt
     2701 tcatttgtag gtccaatcac tcgacaatta ggttactggt tatcagatgg tttaacatgg
     2761 ctttatgaat ttggtggtgc aattggtgga ttaatattcg gattattgta tgctccgatt
     2821 gttattacag gtatgcatca tagctttata gctgtagaaa cgacattaat tgcagatgcc
     2881 actaaaacgg gtggatcatt tatattcccg attgcgacaa tgtctaatgt tgcacaaggt
     2941 ggtgcagcaa ttgcagcgtt ctttattatt aaacaaaata agaagttaaa aggtgtggca
     3001 tctgccgcag gtatttcagc attacttggt attacagaac cggctatgtt tggtgttaac
     3061 ttaaaactaa gatatccatt tattggcgct atcgttggat caggtattgg ttcagcatat
     3121 attgctttct tcaaggttaa agcaatcgca ttaggaactg ctggattgcc aggatttatt
     3181 tcaatcaatc cagtacatgc aggatggtta cactactttg ttggtatgac aatatcattc
     3241 atcattgcta taacagttac tttaatttta tctaaaagaa aagcaaataa agaagttgta
     3301 gaataaaaaa gaggtatgac agtaatcaaa gtatcagtca ttgcaaatgc aataggatga
     3361 aactttaagc tgtcatacct tttaatttag ttataatttc tgtttacgcc ataagaaaat
     3421 actaacaatt acgacaagaa ttaatgaaat acctaccaca ataatccaag atatataact
     3481 atggtcatca attggcaacg gcacattcat accgaagaaa ctaaatacga gtgttggtaa
     3541 cgttaatagt accgtgaaaa gtgttaatgt cttcataatc gtattcatat cattagataa
     3601 taaggaagcg tatgatgttg tgatactttc taagatacgt tgatgtaatt ctgttgtttc
     3661 gatggcttgg ttattttcaa tgattaaatc ttctagtaat tcttcatctt cttcaaaacg
     3721 tttaattgca ggtaaacgga ataacttttt aataattgta tcgttacctt ttaaggcagc
     3781 taaaaagtat actaaacttt tttcaacttc cattaatttg aaaagttgtt tgttagtaat
     3841 gttatttttt agttctttct caatacgaat tctactttta ttaagtaatc gtaagttacg
     3901 attataatgg tcggcaatag tagttaaaat ttctaatgca aatctgctgt gaaattttaa
     3961 attaatatca cgcttaggta aattttctaa aaattcattt tcagcgtcac aaactgttac
     4021 gataataccg ttgccaataa taatgcctaa tggaatcgtt acaaaagata gaacgctacg
     4081 attagttgaa ttgacgatag gtaaatcaat aatgattaat gagtaaccag tatcttcgtc
     4141 atattcaata cgggaacttt cttctgagtc caaagggtca cgtataaagt cttcaggtat
     4201 attatattgt tccataagat tttcaatttc ttctcggtct ggttctacta cattaatcca
     4261 tgacgctgta tgatctaatg gtgtttttat aatatgttta tctatatctt cagaatgtct
     4321 gaatgaagta atcatgatat atagttcctt tctaaaggat ttaggaaaag cgagtattac
     4381 ttaagttagt gaatcaaggt ttgatttatg caaacggttt tctgccatta ttcttaatgg
     4441 accattaaaa ataatattat gcacatgttt agctaattca tcaactgaaa tgggctgttt
     4501 gtccattacc cagtatttaa taattgaaat tgttgctccg gaaacgtaac tgtaaaaata
     4561 catctctgga acgcctccga tggaatgatt gatactaata taacgttgca tattattttt
     4621 aatatgttcg ttgattttca attctaattg actcgtgcgt tccaattgta aaatagtatg
     4681 ataaaactct aaattatcat gaatatgttg gaaaatattt ttgagtatat tatttacaaa
     4741 agtattcgca atgtcttcaa ttgaagaacc gcgtaacaaa tcaaattgag tatatgtcgt
     4801 tagttctgaa atatactcat cttccatatc tgcgagtaac atatatttat cctcgtaatg
     4861 taagtaaaat gtccctctat tgatatcagc gcgtgttgtt atgtcgcgaa tggtaatctt
     4921 ttctaaatct ttttcttgta gtaatttagt gaacgcttgt ttaatggagg actttgtctt
     4981 tctaattcgc ctatcttctt tcaaaaataa atcaacctcc tcgtgttatt gaacaatgcg
     5041 ggtgtatttg tctattaata aacgtattga tgaacattgt gtattgttta ttcccactta
     5101 catattataa tagacaacat gcgaaatatt caacacattg tctaataagg aggtaattaa
     5161 taaaatgaat atatttaaaa ataaattatt atggattgca ccaatagcaa ctatgattat
     5221 cttggtaatc ttttctttag ctttttatcc tgcatataat cctaaaccaa aagatttacc
     5281 aattggtata ttaaacgagg ataaaggtac aacgattcaa gataaaaatg ttaacattgg
     5341 taaaaaatta gaggataaat tattagatag tgattctaat aaaattaaat gggttaaggt
     5401 tgatagtgaa aaagaccttg aaaaagattt gaaagatcaa aaaatctttg gagtagctat
     5461 tattgataaa gacttttcaa aagatgctat gagtaaaaca caaaaagtag ttatggatag
     5521 taaaaaagaa gaaatgcaac aaaaagttgc ttcaggtgaa attccgccac aagtggttca
     5581 acaaatgaaa caaaaaatgg ggaatcaaca agtagaggtt aagcaggcta aatttaaaac
     5641 gattgtaagt gaaggatcaa gcttacaagg ttcacaaatt gcatcagctg tattaactgg
     5701 tatgggtgat aatattaatg ctcaaattac gaagcaaagt ttggaaacat taacgagtca
     5761 aaatgttaaa gtcaatgccg cggacatcaa tggtttgacg aatccagtaa aagtggataa
     5821 tgaaaaactt aataaagtta aagatcacca agcaggtggt aatgcaccat tcctaatgtt
     5881 tatgccaatt tggataggtt caatcgtaac gtctatctta ttgttctttg catttagaac
     5941 tagtaacaat atcgtcgtgc aacatcgtat cattgcttca attggacaga tgatatttgc
     6001 agttgttgca gcatttgcag gtagctttgt ttatatttat ttcatgcaag gcgttcaagg
     6061 atttgatttt gaccatccaa atcgtatcgc aatttttgta gcatttgcga ttcttggttt
     6121 cgtgggcctt attttaggtg ttatggtatg gctaggtatg aagtcagttc caattttctt
     6181 cattttaatg ttctttagta tgcaacttgt aacgttacct aaacaaatgt tgcctgaaag
     6241 ttatcaaaaa tatgtatatg attggaatcc attcacacac tatgcaacaa gtgtaagaga
     6301 actattatac ttgaatcatc atattgaatt aaatagtaca atgtggatgt ttatagggtt
     6361 tatgattttt ggcgctgtat caagtttagt ttcagctatt gttagaaaac atagcacaaa
     6421 acgtactgaa gtgccgtcat aatttaataa atagaaataa tagctataca agacgtttgt
     6481 agcgagcgtc atggtatagc tattttattt gtatagcgct tgatgtaaag ttattttagc
     6541 tgtcatacta tgtgctaaac caaattcagt tgtgtcatat cttcgctttt ttgtcactgt
     6601 tataataggt ataatcggat ataatgaaaa ggtgagggag gattcaaaat gcgtaggtta
     6661 ttatattcat ttctttttta tatgatcata ggtttattta gtggcttttt ctatagagag
     6721 ctaacaaaag catatgattt tactggtaca acacaattat cacttgtaca cacacataca
     6781 cttattttag gtatgtttat gtttttaata ttattaccat tggagaagtt atttaaatta
     6841 tcaagttact acttatttaa ctggttcttc tatgtatata acataggtgt tatcgttact
     6901 ataggtatga tggtgacaaa aggattcttc caagtcactg gtaaatcata ctctccagaa
     6961 gcatttgcag gctttgcagg tattgggcac acaggtatgc ttgcaggttt attgttgcta
     7021 tttttccttt taagacaagc aattcttaaa gaaccacgtg atgagtcttt taaaaaataa
     7081 tataactaga actaaaaaat atacacatct ttcaaaggaa cattgttgtt tcttactttg
     7141 aaaggtgtgt ttttatattg tgatgggata gttattgata attcatggtt aac
//
LOCUS       DBNENW010000125         7052 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNENW010000125 DBNENW010000000
VERSION     DBNENW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.126"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            102..908
                     /locus_tag="AC1LW8_002112"
     CDS             102..908
                     /locus_tag="AC1LW8_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP4773918.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(971..1627)
                     /locus_tag="AC1LW8_002113"
     CDS             complement(971..1627)
                     /locus_tag="AC1LW8_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIP4773919.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1740..2213
                     /locus_tag="AC1LW8_002114"
     CDS             1740..2213
                     /locus_tag="AC1LW8_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIP4773920.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCISELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2336..3547)
                     /locus_tag="AC1LW8_002115"
     CDS             complement(2336..3547)
                     /locus_tag="AC1LW8_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIP4773921.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3729..4388)
                     /locus_tag="AC1LW8_002116"
     CDS             complement(3729..4388)
                     /locus_tag="AC1LW8_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773922.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDENGGA
                     N"
     gene            complement(4448..5590)
                     /locus_tag="AC1LW8_002117"
     CDS             complement(4448..5590)
                     /locus_tag="AC1LW8_002117"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIP4773923.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVQFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5848..6234
                     /gene="gtxA"
                     /locus_tag="AC1LW8_002118"
     CDS             5848..6234
                     /gene="gtxA"
                     /locus_tag="AC1LW8_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HIP4773924.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            6368..6475
                     /locus_tag="AC1LW8_002119"
     CDS             6368..6475
                     /locus_tag="AC1LW8_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIP4773925.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         2335 a         1098 c         1309 g         2310 t
ORIGIN      
        1 aaaatataaa ataatcatct aaatgaagca gttaactata tatattagat aagctatggc
       61 ataataaata acattttaat ttgagtaaag aaggtatggg tatgaagata gggacaatcg
      121 ccgatttaca tattgatcgt cataataaaa aaacatccga agattactta gaggcattgg
      181 ttgaaatagt taagtataag aaattggata tattgttgat tgcgggagat atctcaaatc
      241 attatcaatt gacgcaccaa tttataacgc aattgacaaa acaattagat atacctgtga
      301 aatttgttcc tggaaaccat gatttatggg aagttgaaag tatgactacg caagacattt
      361 ggaataatta taagagcatg tcacagtgct tggtaggaaa accatttata gtaaatgaag
      421 aatgggcaat aataggacat acaggttggt atgattatag ctttgcagca caacgatttt
      481 cattagaaca tttacaaaaa gggaaacatt atggtgcgac ttggcaagat aaagaacgaa
      541 tatcttgggg catatcagat caaaatttat ctaaaatagc ggctgaacaa gtgaaaaaag
      601 atatattaga agtaggaaat agacgagtga ttttagtcac acatgttgtg acgcaccctg
      661 atttcattgt tcctatgccg catcgtatat tcgattttta taatgcattt attgggacaa
      721 gtgatttcaa tcctttgtat gcgatgttcg atataccata tagtattatg ggccatgttc
      781 attttcgtaa aagtgtgata gatgatggca gatgttatct ctgtccgtgt ctaggctatc
      841 caagacaatg gcgttcagaa gatatttacc aggaaataaa tgagacgata caaataatag
      901 aaatttaaaa tgcgcaaacc tgacccagtt tgcgcatttt atgttttaca cacgcgagta
      961 atgtgtttac ttacgtgtgt ttattttgtt gttgattttc aattgtatat gaatgtggtt
     1021 gcacataaat gcactttctt cctggtgaat taaagctgta ttccattttc tctttacgaa
     1081 ttttaataat ttgtttgcga tttggaatga tggcaggtaa aactaggcca cgacgaatat
     1141 gactccaaag tggatcgata taatagtaat agtaatcatc gtacccaatg agtaacgtta
     1201 cgtgaatatt tgaaacttgt ttcgctggct tgttatcaaa gcggaaaaca cgtagtaatg
     1261 gtttagagcc aagattagta tggtatatta acacaggttg accttgatcg ataatacctt
     1321 taagatcttc taaggattta ccagtgccgt ctacgatatt aggattgtat ttttgtaaaa
     1381 atggtacata tgcttctgga aatatcgttt gatgataatt gccaagctta atgaataagt
     1441 gatgtccaac ataaccttta tgtggattgt tcggatgtgt cggccaatgt ctcataattt
     1501 ctgtagcagg gatatgttgg ttattgtatt gcaacatcat ggctgcggaa acaccttcac
     1561 accccatgac catagggata ggaaatagct gactgatagg tttaactggt aatatttttc
     1621 ggttcataat atagtcctcg cattgattca ataaatattt aatataatta tatagcgtca
     1681 atgcaaaatg tcctaaacat atgttttaca tgagtgaata aaattaatgg agtgataaaa
     1741 tggaatatca attacaacaa cttgcgtcgt taacgttagt aggtattaaa gaaacgtatg
     1801 aaaatggacg acaggctcag caacatatag cagggttttg gcaaagatgt tatcaagagg
     1861 gagtaattgc ggatttacag ttaaaaaata atggtgattt agccgggata cttggcttat
     1921 gtatatctga attagacggt aagatgtcat atatgattgc agttaccgga gataatagtg
     1981 ctgatattga aaaatatgat gtcataacat tagcaagttc aaagtatatg gtatttgaag
     2041 cacagggcgc agtacctaaa gcagttcaac aaaaaatgga agaggttcat cactacatac
     2101 atcaatatca agcagatacg gtaaaatcag caccattttt tgagttgtat caggatggtg
     2161 atactacaag tgaaaaatat attacagaaa tttggatgcc tgttaaaggg tgattgaata
     2221 gaaatgttct tgatatgaag gtgtaatttt agttgaagat tcgatctcat aattcataaa
     2281 gaagaaccct gacaaagata gatatctttg ctagggttct aattatgttg aagtgttatg
     2341 cgacatgttg ctttttaatc attttaaagt aaatgatttg tagactgact aatagaatgg
     2401 ctgtaatgaa gataataatc atatatggtg atgtattaaa ttcgcctttt aagccaacta
     2461 atggtgcaac agcgccacct aagataaatt ggaataagcc aagtaaactt gatgcgttac
     2521 cactgccacc tgttcgttct tccatagcca ttgtgaaacc aagtggtcca attgacgtca
     2581 caggacacac atttaagaag aatgcgatga gtaagaccca aagtggtaaa tggaatgtaa
     2641 gtgtcaggat aattaaagca acacctacca cttgtataat agttaaaatg attaataata
     2701 tgtggcgatg taatttttct actaataaag caacgacttg actgacaatg attaaaccta
     2761 caccgttaac agcaaacatg atactaaatt gttggggtgt catattatac aatttttgag
     2821 taataaatgg cgatgcagat gaataactaa atagcattac ataagttaaa ccttgtaata
     2881 gcattggaat aataaatgct ggttttttca aaagacttcc aaaatcttta atgacgctac
     2941 taaaattcac ctgctttaat tcatctttag atgttttagg taattgagaa gcgacgccaa
     3001 ttaaaatgat gagtgccaca atagttaaaa ttgtgaaaat agaacgccat gttgctacgg
     3061 ataaagctaa tccaccagct aatggtgcaa gaatagtgat gatgccatta actaccatta
     3121 aagatgctaa aaatttagcg agtgcgttgc cactaaattt atcaccagca gaggctttgg
     3181 caatcacgat gacgccacca ccagttaaac cttgaataaa tcgtaaagtt aagaataatg
     3241 gcaattgatc aacaaacata gacaaacctg ataccaaaat gaaaatgatc aaaatggata
     3301 cagcaatccg tttgcgacca aaagcatcgg ataaaggtcc aaagataaat tggcctagtg
     3361 caagaccaat cattgtgaat gataatgtta attgaatttc tgatgtagta gaaccaaaat
     3421 caccttgcac tttaggtaat gatggtacgt acatatctat agttagtggg ccaaaagcgg
     3481 tcatgacgcc taacgtaatt agcaataaaa atgaaaaatg cttcgttttg gttttcgttg
     3541 attgcatgat cagactcctt taaatgatat aattatataa attatatacg ttttgtgtgt
     3601 atttgtatgt agttagaata caagtttata taaaaaatcc ctatactctt aatgcatcat
     3661 cacgaataac gtagtgatac agataaagta tagggaatta atgtttatca tcaaagcagg
     3721 tgtaataatt aattagcgcc accattttca tctttaaata agctttctaa tttagattta
     3781 atactgtcga attttgtatt aatatcattt aacatgcttt caattttacc ttggcttgca
     3841 ttgtttttaa gtgcattatt tgcctctgtc aatttgttga tttggtcttg ataaaattga
     3901 gttagcttag aatcagcttt gttttgttct tgctgcttca agttgtcgat ttgattttgt
     3961 aaatttttaa taatgtcatc agttttttgg ttgtttttaa tattttcttt agcattatcg
     4021 acttgctggt taatttggcc gtcttgattt tcaacttgtt ttttataatt ttgagagact
     4081 ttagggtcat tactaacttt atcgctatca ttgttaagta aggcgcgtgt gacaaaaata
     4141 gcgacaataa ttaagagaat agcaataatg atatataacc atttttttga acggcgggat
     4201 ttctcgtttt cttcttcaag aatttgttct cttcgataat ctctaccgtt atagtatcgt
     4261 tccgggtgtt cttcaggatc gcctttagca tattgttgtc tatacgatgc gtcttcagat
     4321 tgacgacgat gattttgatt ttgattgtcg tattgatatg aatcacggta tttatcagtt
     4381 cttttcatgg gtttcacctc attcaagttt taactgcaaa taataaagct gatgatttac
     4441 tttgacatta ttgcaatttc agaatacgag caatgtcagt tgcagtgttt aataaatttt
     4501 gttcgctatt ttgtaggaca tcttctaaag tgctaggtga agagattata gaataggcac
     4561 tatcaatacc gaaatcgtaa acatgttgat aattttcgcc tagactgcca caaatcgcga
     4621 tgacaggaat atgatattgt tttgcagcta acgcaacgcc tacgggtgtt ttaccaaaga
     4681 tggtctgata atccatgcgt ccttctccag taataacgag gtctgcatct ttaattcttt
     4741 gatgaaaatc tgtaatgtca aagacgacat caataccttt tgttaaagtt gtctcacaaa
     4801 acgctaataa tgctgcgccc ataccgccag ctgcaccaga acctggtatt tgattaacgg
     4861 actttcctgt gcacattttt atcttatcat gataatgcga cattgcgaaa tccaactttg
     4921 gtatcatctt tgcatcagcg cctttttgag gaccataaat ataggtagca ccattttcac
     4981 ccaataaagg atttgaaaca tcacaggcca ctttaaaggt cacctctttt aatcgcgaat
     5041 ctagattggt tatatcgatt tgtgcaatgt gagcaagatt agcaccattc atttgtaata
     5101 agtccccgtt tacatcagta aactgaacgc ccagtgcact tagcatacct gtaccaccat
     5161 catttgttgc actgccacca atccctaaaa taatggtctt agcaccatga tttaatgcat
     5221 ctttaattag ttcaccggta ccatatgatg atgtgtataa aggattacgt tcctcttttt
     5281 ctaataaatc caaacctgac gctgccgcca tttcaataat tgcaatttgt tgttcgtctg
     5341 cgcgtgcata acatgcttca ataggtcgca ttaaagggtc attaacgatg actgtatact
     5401 tagtggcacc tgttgcatgc attaaagcat ctgtggtacc ttcaccacca tcagccatcg
     5461 gaatgatatc ataatgaagg gtattcccat aaacattagt aaatgcctgt tttataatat
     5521 tgccaacttg ctgtgcggtc atactttcct taaatgagtc aggagcgatg acaatttttt
     5581 gtaattgcat agtagacatc ctctcattac tgttgtcgct cttattgtat ataaaattac
     5641 tagtgaacta aagtaatctc tatagtgcta atgagtcacg cgataaatca gttacattat
     5701 aatggcgtat aaggatgaga ggaaagagac taaatttgct gtgaaagggt ataaagattg
     5761 aaataaacat atttgaagtg aagcggattg gcacgcaatt tcgagaacgt agttatgcat
     5821 atattttgat gttgaggagt tacacatatg aaactaacac aaacgcatgc tgaaatttta
     5881 aaatttatca ttgttggcgg cattaatacg ttaaattatt atgttgtcta tttattgctg
     5941 ttaaaattat tacacattga atatatgatt agtcatatta ccggatttct agtcgctttt
     6001 gtgatttcgt attatttgaa ttgttatttt gtttacaggg taaaacctac ttggagaaaa
     6061 ttcattagtt ttccaattac gcagattgtc aacgtaagct tacaaacagt tttattatat
     6121 gtttttgtat cctggctaaa tttgccagcg gaaattgcgc catttgcagg tttagtgatt
     6181 acgataccca tcacatttgt attatctaaa tggattttaa aagatagtaa ttaaagtagc
     6241 gtaaaccact aaaaaacgct ttactcgaac tttaatattg cgtgaaatat aataaataaa
     6301 tgatataata taatggtcgc ctaacatctc gggcgttact ttaacgcaga gaggaggtgt
     6361 atgacggatg ttcaatttat taattgacat catgacttca gctttaagcg gctgtcttgt
     6421 tgcgtttttt gcacattggt tacgaacgcg caacaataaa aaaggtgaca aataaccaac
     6481 acctaaataa atcccctcac tatggcagta gtgaggggat ttggtgtatt tagatgttca
     6541 atttattatg taattaatta tagcattgat gaacaaggtg tgcaatgtat ttttgtaata
     6601 ttacaataaa tgaatttaat tatattagaa ttaagtattt cgaaaaatga tttaatagtt
     6661 gagtcatagg atcattagat aaagcgaata atcaattata gggacattgg gatacaaatc
     6721 aatgtactag actctacaag gctatactca gggacccaac atagagaaat tggatccaca
     6781 gtttctacag acaatgcaag ttggcggagt cccaactaag agaaattgga ttcccaattt
     6841 ctagagacaa tgcaagttgg ggtgggacga cgaaataaat tttgtgaaaa tatcatttct
     6901 gtcccactcc cagaaccact acataataaa tcattagtgg ctctttatca tttctgcccc
     6961 ataaccttgt gaatttttaa aagaaatgtg tcacaaatcc acgcgacatt gataatcgtt
     7021 atcaactaat atatgatgta cttgtaataa gt
//
LOCUS       DBNENW010000126         7027 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNENW010000126 DBNENW010000000
VERSION     DBNENW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.127"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(104..1741)
                     /locus_tag="AC1LW8_002120"
     CDS             complement(104..1741)
                     /locus_tag="AC1LW8_002120"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIP4773926.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNIRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(1900..2778)
                     /gene="hchA"
                     /locus_tag="AC1LW8_002121"
     CDS             complement(1900..2778)
                     /gene="hchA"
                     /locus_tag="AC1LW8_002121"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HIP4773927.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSTLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(3045..4232)
                     /locus_tag="AC1LW8_002122"
     CDS             complement(3045..4232)
                     /locus_tag="AC1LW8_002122"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIP4773928.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            4403..5578
                     /locus_tag="AC1LW8_002123"
     CDS             4403..5578
                     /locus_tag="AC1LW8_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIP4773929.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(5860..>7027)
                     /gene="tuf"
                     /locus_tag="AC1LW8_002124"
     CDS             complement(5860..>7027)
                     /gene="tuf"
                     /locus_tag="AC1LW8_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIP4773930.1"
                     /translation="DRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMI
                     DNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSA
                     ADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEYDFPGD
                     DVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGR
                     GTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGV
                     AREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFRTTDVTG
                     VVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK"
BASE COUNT         2181 a         1323 c         1056 g         2467 t
ORIGIN      
        1 gtacatgttg ctgatatttc tgtaaatgga aattctagtt gtattaattg attttagtaa
       61 tttatagcgt ttattattgc taattactgt caaattaaat tttttatccc tcaactctta
      121 aactctggat atctttcatt atattagctt ttttataacc atggatatca tgtaaagcct
      181 tataagcttt aaataatgtt tcataccttt gtactttttc cgcttctgga ttaatttcgt
      241 ataaaattgg ctctttcata gcatcaactg catcattaat tgaattatgt gcgccaccac
      301 tgactgcacc taatattgct gcgcctattg ctggtgcata ctcactatcc ataacaatta
      361 gttttttatt cagtacattc gcatagatat ccatcataac agcattcttt ttaggtatac
      421 cgccacatgc aaataccttt tctacttcca tatgccaatc ttgatactgt tgcataatca
      481 tcttagtacc aaatgctgta gcttctaaat atgctctata aatatcttca tgcttagttt
      541 gtaaagttaa tccaaagata caacctgtta aattgctatc acttaataca cttcgatttc
      601 cattatgcca atcaagagca atgagcccac tttcacctgg catttgatgt tttatctttt
      661 cattcattaa ttcaaataca gtcatatttc tatttgctgc ttcatctaca tatgactttg
      721 gtgcttgctt agcgacatac tcaaacaaat cacctactgc tgattgcccc gcttcataag
      781 caaataattc tggaataatt gctcctttta cagaacctga tatacctggc acttgatgtt
      841 gcttttcatt taacataaga tggcatgtgc ttgttcccat caccatagtc atttctttat
      901 ctttttcaga cccaatacct aataaactag catgggcatc aataataaaa ggacttacca
      961 tagtctcttt tgataatcct aatttctgtg ccattttatc atccagtttc cctactgctt
     1021 caccaatatt aacaaccggt gcagatactt tatcttgaat tacttttgat aatttggggt
     1081 ctattttatc aaataaatca taatgaaacc ctgtttcttc ttcccaaaat gctttgaaac
     1141 ctaatccaca attcgagcgt atatttttat tagttaattt atttacaatc caatcgcccg
     1201 cttccataat ataagccgtt ttttccataa tttctggtgc tcgattcatg acttccatta
     1261 ttttgggaat catccattca ctactaacat tatatccata atggcctaac cacttattat
     1321 tattttcaat agcagtttga tataatttct ctgcttcttt atatgcacca tgatgtttcc
     1381 aaagtttcac atacgcatgt ggattgtttt taaattgttt taaattatgt accgggttaa
     1441 gattttcgtc agtaaaaata atagtagatg aagtaaagtc tataccaata cctactatat
     1501 tgtctggatc tatttttgat tcacgtacta tatatgatat tccttcttcc ataatttcta
     1561 aataatcatt actattttga agtgcatatg tatgtggtat tttcaaacca tttaattcac
     1621 tttcaatgac accatgtgta tatggtttca caaattttga tactacttga ccgttagttg
     1681 tattaattaa aaacacacgg cctgaagctg ttccataatc aattccaatg ctataagaca
     1741 tcatgtttcc tccatatcgc tagttgcggt tattagtttt gcaagcgttt tcttaaatca
     1801 tactcataat aggttctatt ttacaagtat cacttaaatt ttgccaaaaa aaagagccct
     1861 catacattta catatgaggc tcctctaatt aatatttaat tatttatttt gtattgcatt
     1921 taacatttca ttaactgcta attttcctaa ctcatttgaa gctaaaggac tgtcacctgt
     1981 taataattta cgatctttta acgttcttcc tgtcatatcg tcgttaacta cttttaatcc
     2041 ttgtttagtt aataaatcag caactaacca tttcaagcgt ccaggtaaat aacctatttc
     2101 aatatttgca ccttcatcta atgagtcagg gaagacacaa acagagtatc cttctaatgt
     2161 agatttttct ctgttaagcc ctgctgaaag tagtgctgct ggtccatgac ataatgtaac
     2221 tataaagcgg tcattgtcta atgcccaatc taatgtttgt tgaacatcct cactttcaga
     2281 aataccaaca actgcagcat gtccgcctgg gataaagaca gataaataat ctgaatcagg
     2341 tcctaattca tttttaatca catctgctaa tttttttggc tgttttaatt tttctttcaa
     2401 tttattataa gtacttataa ctgcctcgtc ttcagttggc atagcccata attctaattt
     2461 aactggataa ccagataatg tcgcaacatc aacgtcaaaa cctgcttcca ttaaatgatg
     2521 taaaggtaat aacatttcaa caggatgatt acccgtagag aacatttttc cattttccaa
     2581 taatacatat ctctcttcag cagcaatcat caatactttc catttaccat ctttataggc
     2641 acctttgtgt tcaacaccat caaaatctgt tttaggtgct gtatattgac taagggaata
     2701 tggtgatggg aaaaatgcgt tatcttctgc tttatctggc gttggttgct tacttaattc
     2761 atttacatct tgtgacatag tttattccta ctttctctat atagttacta ctagtataga
     2821 catatactac acactataaa catgtattac tattaaaatg ctcattatca aaaataatca
     2881 aaatgaattt ctttgaaaaa aatacaacta aattttacaa gatgctttta aaattaaaac
     2941 tttaattttt gggagcacta aacattgtta taagaactta ctgttaataa actaaacatt
     3001 taaaaaaagc ccacgacaat atttgccgtg ggaataaata aatattaaat caacttcatt
     3061 tcttttccta ctttttcata agccgcaatt gcttcatcta acatgtcttt tgtatgtgca
     3121 gctgtaggca tatttcttac acgtcctgta cctcttggta ctgttgggaa aacgatagat
     3181 ttcacataga caccttcgtc ttttaaacgc ttactaaatt cttgagttgt tttttcatca
     3241 ccaataatta ctggtgtaat tggagtttct gactcacctg tatcatatcc taattttgac
     3301 aatccatttt ttaaatattg tgcattgtcc cataatttat catgtaattc agttgaatcc
     3361 attaactttt taactgcttc agttattgct ttggtatccc caggtgctaa tgatgtagag
     3421 aataagaatg gtcgtgattg tgcttttaac caatctatta actcttttgt acctgctaca
     3481 taaccgccaa cgacaccaat tgctttagaa agcgtaccta tttggaaatc gattttatct
     3541 tgtaaaccaa aatgtttaac cgtaccagcg cctttaccca taacacctga accatgagcg
     3601 tcgtctacat aagttaataa accaaattct tctgcaattt ctacaatttc aggtaattta
     3661 gccacatcac catccatact aaaaacgcca tcagtgatat acatcacttt attgtattga
     3721 cctgattcaa ctgcttcttt tgctttcgca cgtaaatcat ccatgtctga atggttaact
     3781 cgaataattt tagctttaga taagcgacat ccatcaataa ttgatgcatg attaagctca
     3841 tctgataaaa tagcatcatt tttattcatg acagctgaaa tagcagccat attacaatta
     3901 aatcctgatt gataagctat tgcagcttct gttcctttaa attttgctag tgtttcttct
     3961 aattcgtcgt gtaaatctaa tgtaccattg attgtacgaa cagcgcctgc acctactcca
     4021 tgtgtatcaa tagctgcttt tgcagctgat ttcaaatctt catttgttgc tagtcctaaa
     4081 taattatttg aagataagtt aatgtatgat ttcccattga ttttgatttc tggtccgttt
     4141 gcaccttcaa ttgtatctat ttcattatac aaaccatttt cttttagata atttatattt
     4201 tcctctaaaa actcatgtaa tgattgaacc acagtatata atccccctta agtattgttt
     4261 ggtaatacct ttatcataca gtaattctac ataacttgaa agccttatca ttcatagata
     4321 aaatatctca aagcaatcat ttagtacgat aatttcataa aatgttatac tataaacatt
     4381 attttagaaa gtagtgataa gggtgttaga ttggttccaa ctagcaaata ataaagaaaa
     4441 taaaacaatt caactcagac gttatttaca ccaataccct gagttatctt ttgaagaatt
     4501 tcaaacacat gattatattg ttaaccaatt aagccagtta tcttgtgata ttgaaactcc
     4561 tatcggccgc aatggtatca aagccacatt taaaggcttg ggcacaggac ctaccattgc
     4621 attaagagct gactttgatg cgttacctgt cgaggaatta aacgatgtgc cttataagtc
     4681 taaaaatcca ggatgtatgc atgcgtgtgg gcatgatggt catacagcaa ttttacttac
     4741 tgtagcagag attttagatg aacataaaca tttattagaa ggtaatgttg tattaatatt
     4801 ccagtacggt gaagaaatca tgccaggtgg ttctcaagaa atgatagatg ctggatgtct
     4861 ggaaaatgta gatagaattt acggtacaca tttatggagt ggttatccta ctggaactat
     4921 tcattcacgt gcgggggcaa tcatggcctc tccagatgaa tttagtgtca caattaaagg
     4981 tcgtggcggt catggtgcca aacctcatga aaccattgac ccaatagtaa tcatggcaga
     5041 gttcatttta agtgcacaaa aaataatttc acgtacgatc gatccagtta aacaagctgt
     5101 actttcattt ggaatgattc aagcaggcac cacagatagc gttattcctg atcaagcatt
     5161 ttgtaaaggt acagttagaa ctttcgattc agatatacag aatcatgtaa tggacaaaat
     5221 ggataagctt ttacaaggtt tagcaatagc aaatgatatt aactatgatt tgaattatat
     5281 taaagggtat ttgcctgtcc ataataacga gaaagcatat caagttatta aagaagcaac
     5341 aaatgactta catgtacgct ttaatgaatc agatttaatg atgattggag aagatttttc
     5401 acattatttg aaagtacgac caggtgcatt ctttctgact ggttgtggta atgaaagtaa
     5461 aggtattaca gcgccacacc ataatcccaa atttgatatt gatgaaaaat cattaaaata
     5521 cgctgtagct gtatttttaa aaatcattga attagagcaa gtatttaaaa caaattaagc
     5581 atttaaatta attgaaagat atgaatctga gacataaact aatgaaatgc tcccttcaaa
     5641 gtagacattg aaaaaatgaa aactttgaag ggagcatttt acaatgtcca agactcaaca
     5701 attttattta aacagtatta tcagttataa gttttattag tcagtctttc atcaattatt
     5761 gttcttctaa atcgaatagc cattacaaaa tttacacatt aaaaaaagac ccctcgattg
     5821 agggatcttc tttttatata aaatctaaga aattagaaat tatttaatga tttcagtaac
     5881 aacgcctgat cctacagtac gtccaccttc acggattgag aaacgagtac cgtcttcaat
     5941 cgcgattgga gcgattaatt ctactgtcat ttcaacgtta tcaccaggca ttaccatttc
     6001 agtaccttct ggtaagtgaa caacaccagt tacgtcagta gtacggaaat agaattgtgg
     6061 acgatagttt gagaagaatg gagtgtgacg tccaccttcg tcttttgata atacgtatac
     6121 ttctgctttg aattcagtat gtggtgtaat tgaaccagga gcagctaata cttgaccacg
     6181 ttgtacgtct tcacgagcaa caccacgtaa taatgcacca atgttgtcac cagcttcagc
     6241 gtagtctaat aatttacgga acatttcaac acctgtaaca gttgttttag atgtgtcatg
     6301 taaaccgatg atttcaactt cttcaccaac tttgatttga ccacgttcaa cacggcctgt
     6361 agcaacagta ccacgaccag tgattgagaa tacgtcctca actggcatca tgaatggttt
     6421 gtcagaatca cgttctggag ttggaatgta agtatctaca gcttccatta attctaagat
     6481 tttttcttcg tattgagcat cgccttctaa agcttttaat gctgaaccag cgattacagg
     6541 tacatcgtca cctgggaagt catattcgct taataagtca cgaacttcca tttctactaa
     6601 ttctaataat tcttcatcgt caaccatgtc aactttgttt aagaatacta ctaatgctgg
     6661 tacaccaacg ttacgtgata aaagaatgtg ttcacgagtt tgtggcattg gaccgtcagc
     6721 agcagatact actaagatac cgccgtccat ttgagcagca ccagtgatca tgtttttaac
     6781 gtagtcagcg tgtcctgggc agtcaacgtg agcgtagtga cgtttgtcag tttggtactc
     6841 aatgtgagaa gtattgattg tgataccacg ttctttttct tctggagcgt tgtcaatcat
     6901 gtcatatgat tgtgcaactg agtcaccatt ttttgctaat acagtagcga ttgctgctgt
     6961 taatgttgtt ttaccatggt caacgtgacc gatagtaccg atattggcat gttctttaga
     7021 acgatcg
//
LOCUS       DBNENW010000127         7006 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNENW010000127 DBNENW010000000
VERSION     DBNENW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.128"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(48..998)
                     /locus_tag="AC1LW8_002125"
     CDS             complement(48..998)
                     /locus_tag="AC1LW8_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIP4773931.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNDLIENEVDAKKGY"
     gene            complement(1004..1459)
                     /gene="bcp"
                     /locus_tag="AC1LW8_002126"
     CDS             complement(1004..1459)
                     /gene="bcp"
                     /locus_tag="AC1LW8_002126"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIP4773932.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            1540..2829
                     /locus_tag="AC1LW8_002127"
     CDS             1540..2829
                     /locus_tag="AC1LW8_002127"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIP4773933.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            3122..4216
                     /locus_tag="AC1LW8_002128"
     CDS             3122..4216
                     /locus_tag="AC1LW8_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIP4773934.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(4306..4399)
                     /locus_tag="AC1LW8_002129"
                     /pseudo
     CDS             complement(4306..4399)
                     /locus_tag="AC1LW8_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4408..6144)
                     /locus_tag="AC1LW8_002130"
     CDS             complement(4408..6144)
                     /locus_tag="AC1LW8_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HIP4773935.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIIAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(6435..6977)
                     /gene="ntdP"
                     /locus_tag="AC1LW8_002131"
     CDS             complement(6435..6977)
                     /gene="ntdP"
                     /locus_tag="AC1LW8_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HIP4773936.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWNERYKKIRQY"
BASE COUNT         2293 a         1215 c         1016 g         2482 t
ORIGIN      
        1 ataactgtag tattgcgcgc gtgtcaatat ttacatgatg atttcattta atagcctttt
       61 ttagcatcaa cttcattctc aattagatca ttcttattga gaaaattaac tagattgttt
      121 ttaaaaatat ctaataagtc atactttgcc tcataatcat taccagttat atgcgctgtt
      181 atagttacat tatccaattc atataattca tgattaggtt tcaaaggttc attttcaaac
      241 acatctaaat atgcatgtcg aataacttta ctttttaata cttctattaa gagcgcttct
      301 ttaactatgc tacctcgtcc tatatttata aaaagtgctt catctttcat taattcaaaa
      361 tgttttttct ttagtaaatg aatcgtttct tgcgtttctg gtaaagcatt tataataatg
      421 tcagcatttg gtaatgtgct ttcaagtgat tcgatagtat atatctcgtc aaactcatct
      481 ttgttctgac ctgacttgct cagaccaatt aaattcatat taaaagcctt tgctaacttc
      541 gcagttctag tagcaattgc acctgtacct aaaaataaaa ctgtttgtcc tgataggcgt
      601 ttaccagtta ttttcgaatc atatatatgt tgtcgttggt tatcatatga tagtttcatc
      661 tttttataat catctaaaat gaaagctaaa atgtattcag ataattgttt agcttgaaca
      721 ccttttccat tagttaaaag tatgccgtga tctgcaatat aatctaacgg caatgtattt
      781 acacccgttg caaaccatgc aatccatttt aaattcgggc aacgttgtaa aaatgcctca
      841 ttgataccgc catcataacc aactaatata tctaaatcag ccaagtcgct ctcaggtatt
      901 tctgatgctt ttttataaaa tttaaaatct aaatctgaaa agcgttgttg taattcagtt
      961 tcaacttcac gcatacgatt caacccaaca actttcatat atgtcacccc aaaatgtttt
     1021 taagttcttc tatttgtgtt ttaaccttaa ccttctcgat aacatctaat actttacctt
     1081 gttcatctat tataaaagtc gttcttacaa tgcccatact ttctttgcca aatgattttt
     1141 ttaactgata tacgccagtt tctttagcta atttaaaatc ttcatctact aataaatcga
     1201 aattcaatcc gtgtttctca ataaaatttt ggtgtttttt ctttgaatca ccgcttatac
     1261 catatactgc aacatctaaa tcattgaaca tttctaaatt gtctctaaag tcacaagctt
     1321 ctgtggtaca agtaggtgta ttatctctag gataaaaata tataatcgcc tttttacctt
     1381 ttaatgtatc atttgtaatg acagttccgt cttgattttc taatttaaat attggaaatt
     1441 gttctccttt ttgcaacata taatcacctt tttcttcatt ataataattt tatgatacga
     1501 tattagatga aaatattaaa ttaaaagagg ttgatataaa tgaattttag tgaaagtgaa
     1561 cgtttacaac aactttcaaa cgaatatatt ctaggcggtg tcaattcccc ttctcgttct
     1621 tataaagctg taggaggcgg tgcacctgtt gttatgaaag aaggacacgg tgcatattta
     1681 tatgatgtcg atggcaataa atttattgat taccttcaag catacggtcc aattattacg
     1741 gggcatgcac atcctcatat tactaaagca attcaagaac aagctgctaa aggtgtttta
     1801 tttggtacac cgactgaatt agaaattgaa ttcagcaaaa aattacgtga tgcaattcca
     1861 tctcttgaga aaattcgctt tgtaaattct ggaacagaag cagtcatgac aacaattcgt
     1921 gttgcacgtg catatactaa aagaaataaa attataaaat ttgctggatc ttatcatggc
     1981 cattctgatt tagtattggt tgcagcaggt agcggcccat ctcagctcgg ttctccagac
     2041 tcagctggtg ttccagaaag cgtcgcacgt gaagtcatta ctgtaccttt caatgatatt
     2101 aacgcctata aagaagcaat tgaattttgg ggtgatgaaa ttgccgcagt attagtagaa
     2161 ccaattgttg gtaactttgg aatggtaatg cctcaacctg gatttttaga agaggttaat
     2221 gaaatttcac ataacaatgg gaccctagtg atttatgatg aagtaattac tgcattccgt
     2281 ttccattacg gtgccgctca agatttatta ggtgttatcc ctgatttaac tgcatttggt
     2341 aaaattgttg gcggtggttt accaattgga ggctatggtg gacgtcaaga tattatggaa
     2401 caagtagcac ctctaggacc tgcatatcaa gctggtacaa tggctggtaa cccgttatct
     2461 atgaaagcag gtattgcatt actcgaagta ctagaacaag acggtgttta tgaaaaatta
     2521 gacagcttag gccaacaact agaagaaggt ttacttaaat taatcgaaaa acataatatc
     2581 acagctacaa ttaatcgtat ttatggatct ttaacattgt actttacaga tgaaaaagtc
     2641 acacattatg atcaagttga acattctgac ggcgaagcgt tcggtaaatt tttcaaatta
     2701 atgttaaatc aaggtatcaa tttagcacct tctaagtttg aagcttggtt cttaacaact
     2761 gaacatacag aagaagatat tcaacaaact ttaaaagctg cagactatgc ttttagtcaa
     2821 atgaaataac ttttaaggaa gtatattttt aattgaatta aaataaaaca aatgctcaca
     2881 tagtagattc attttatatc gttaactatt aacgtttaac ttgatatgtc actatgtgag
     2941 tttttatttt acaaatttat actcttttaa tctctgacta aagttttaca tcgctatcag
     3001 tattatgtat gatttatttt aacaactata aataagcttg aattttgtaa ctagtaagtg
     3061 tataacagta atgaatacga atattagata ttcaataaac ttaaaagggg cgtcattatt
     3121 tttgagacta ggagctcgga tttttaaaac tggtatagcc attattttag ctatgtctat
     3181 cgcttcttta ctaccggatg atgttggtct gaaagcctta gctggtgtca gtgctgttgt
     3241 tgcaatgcaa cctagtattt atcgttcatt taaaacagtt tctgatcagg cattaggtaa
     3301 cattataggt gccatacttt ctgttacaat ggtaacaatt tttagtgata attttatcat
     3361 tatgggtgtt accgtcatag tacttattgc tattttattt aaatttaatt tagcacatgt
     3421 tgctactttg gcgagtgtaa cagcattaat cattatggga caacataccg gttctttcta
     3481 tattaccgcc ttttacaggt ttgtacttgt aatgattggt gtcataagtt catctctagt
     3541 taactttgtg tttctaccac ctaaatttga aacaaaaatt tactataatt cattaaatat
     3601 atcctcagat atttttatgt ggtttaaatt agtattaaat gatacgacgg agtttaataa
     3661 tattaaacaa gatagtcata atttaaaaca acgtgtcgaa aagttagaga aaatttatga
     3721 ttactatagc gaagaacgtc ctattacaaa aaaacatatt caccaacaga atagaaagaa
     3781 aatacttttc agagaagtag ttcagacgac tagacaagct tatgaagtat taaacaaatt
     3841 gtcgcgttat caaaacgact tatatttact taataacaat ttcctattac aaatcaaatt
     3901 agatttagat tcattaactg cttttcacga gcaaatttta gctagccttt ctaaaaaagc
     3961 acgttataat gtgacacatg ttgattatga gttagataat ccgcagaaaa aagatttatt
     4021 gtctacattt cagcatgagt taataaatca tccttatcaa acggaatatt cgtttgccaa
     4081 tgttatgcaa attgttgcag ccatcgaaga gtatcgacac catttagagc atttagaccg
     4141 cataagaatt agctttttca catatcatag atctgatgca gacattgaaa ttgttgaaga
     4201 agactttgat ttataacatt aaaaaaattc ctataattaa ttccacaatg agataaagta
     4261 atattgaatc tcgaaattaa atttaaaaaa gcagtaagat tattttcaat taagaaaatc
     4321 tcttactgct ttttcttatg ttatgacccc accatcctgt tacaaatttt ctcttcagga
     4381 ttgtactgaa gtcaaacaac taatacttta taagttttga atgctatata aatgctcgta
     4441 agcaccttgt tttgcaatca attcacgatg cgtacctgtt tcaacaatat gtccattttc
     4501 aattacgaca attttgtcag catgtgtaat agtggacaag cgatgcgcta tgataagtgt
     4561 cgttcgatct ttactcaaca catctaatgc ttcttgaata atggattcac tttctaaatc
     4621 aagtgcactt gttgcttcat ccaagataag aattggcgga ttatttaaaa atattctagc
     4681 aatcgataat ctttgttttt gaccacctga taatttaaca cctcgttcac ctacttcagt
     4741 gtcatatccc tgtggcaagt tcataataaa gtcatgtgca ttagccattt tcgccgcttc
     4801 aactacttct tcatctgttg ctgttggacg accaagtaaa atattttcct taactgtatc
     4861 agagaataaa atattatcct gttgcaccaa tcctatttga tttcttaaac ttcccgttaa
     4921 aaaatcttta atgttgtgac catctattaa aatttgccca gaagttacat cgtaaaatct
     4981 cggtattaag ttaattaatg ttgatttacc accaccactc atacctacga aagcaactgt
     5041 ttctcctttt tcaatactca aattaatatc ttttaaaatt ggagcttcgt tatcgttata
     5101 ttgaaaacta acatgatcaa tatcaatacg accttgttta atttcaatag gttgagcacc
     5161 aacaccattt ttgatgtcat aatcttcatc aattaattgg aatacacggt ccattgaagc
     5221 aaaactttgc gttaaagttg taaatgatgc gactaaacga cgtaaaggcc cgaacaataa
     5281 ctccaagtat ccaacaaatg ctgcaagtgt acctactgtg attgatccag aaatagcaag
     5341 atatgcacca acaccgatga caattattgg tccaatatct gtaactgtat taattgcggc
     5401 aaaggaatag gcattccatc ttgtatgttt caaagcacgt gttaagaaat tagtgttctt
     5461 tttatcaaag tttttcgctt cattgtcttc aatcgcaaaa cttttaacga ctgaaatacc
     5521 ttgaacacgt tcatgcaaga atccttgaac ctcagctaat gcttgcgatc tttcacgcgt
     5581 caattttctt aatcttccaa agaaaacgta caccgttaaa atgtaaaatg ggaagataaa
     5641 cagtgctgct aaagtcaatt tcacatctaa aaagaacatt atggatagtg caataataat
     5701 tgttatacaa tctaaccaaa tattcattaa cccggttaaa atgaaatctt ttgtttgttc
     5761 aacatcatta atcactctag atattacttg acctacttga ttattagcat aaaatctcgc
     5821 acttaaagct tgtaaatggt tgtataactt tttacgtata tcatacaata ttttattact
     5881 tgtccattgc gccaaatatt gacgtataaa ttcaattggt ggtctcacta ttacaaaaat
     5941 aaataatgcg ataccaatgg caatagttaa atgatgaact ttttcatcag tcgttagtgc
     6001 gtggttatta atcacgccat ctattgcata tttaattaaa agtggtataa gcattggtat
     6061 accaaactta attatcccaa caataatcgt tgcaaaaata cgatatttgt atggcttaac
     6121 aaattgcaaa tatcgtttaa tcatacaatc ccccctaatc tattgcccta tcctattcat
     6181 aagcataaaa atgaatagag gttggataca taatttgtag atgtaaattc ttcttacaat
     6241 ttacattttt aaaattaaga tatctcaacc tctgtcatat tcatgtgatt aaatacgttg
     6301 tcttattcgt atgttatatt ctattcaatt taatctatgg atactgtgtc cccacacgac
     6361 agcaaaagtt atcatacttc tttacaacac taagtcaata taaatgattt aatcagtatt
     6421 tacactttat ttgcttaata ctgtctaatt tttttgtaac gttcattcca aactttgata
     6481 aaatctggcg cgaatgggcc cttcttctgt tctatccatt gttgaagaat gtccacgttg
     6541 cgtcttaaaa taatatcaat atcatgcgga taattcattt gattcatatg ttgttcatat
     6601 tcatcttcat ctaataaatg atactttccg tttggatata ctttaatatc taaatcatag
     6661 tctatatatt ttaatgcctc ttcatcacaa acaaatggtg atgacaaatt gcaatagtaa
     6721 taaattccat cttctctaaa catgcagata acattaaacc aatattctga gtgaaagtaa
     6781 acaattgccg gttcacgtgt tatccaagtt cttccgtcac tttcagttac taacgtatga
     6841 tcattgccac caatgacaac atgatcagta ccctttaata ttgttgtttc agaccaaacg
     6901 cgatgaatct taccatcatg tttataactc tgaattttaa tgttttcccc ttctttaggt
     6961 atggattctc tgaccatact ccacaccacc ttctgttaat ttaacc
//
LOCUS       DBNENW010000128         6950 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNENW010000128 DBNENW010000000
VERSION     DBNENW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.129"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            41..748
                     /gene="splA"
                     /locus_tag="AC1LW8_002132"
     CDS             41..748
                     /gene="splA"
                     /locus_tag="AC1LW8_002132"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HIP4773937.1"
                     /translation="MNENVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            873..1595
                     /gene="splB"
                     /locus_tag="AC1LW8_002133"
     CDS             873..1595
                     /gene="splB"
                     /locus_tag="AC1LW8_002133"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HIP4773938.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGITLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1653..2372
                     /gene="splC"
                     /locus_tag="AC1LW8_002134"
     CDS             1653..2372
                     /gene="splC"
                     /locus_tag="AC1LW8_002134"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HIP4773939.1"
                     /translation="MNKNIVIKSMAALAILTSATGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPDGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQALNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2493..3212
                     /gene="splD"
                     /locus_tag="AC1LW8_002135"
     CDS             2493..3212
                     /gene="splD"
                     /locus_tag="AC1LW8_002135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HIP4773940.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            3370..4086
                     /gene="splE"
                     /locus_tag="AC1LW8_002136"
     CDS             3370..4086
                     /gene="splE"
                     /locus_tag="AC1LW8_002136"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HIP4773941.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4237..4956
                     /gene="splF"
                     /locus_tag="AC1LW8_002137"
     CDS             4237..4956
                     /gene="splF"
                     /locus_tag="AC1LW8_002137"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HIP4773942.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5021..5155
                     /locus_tag="AC1LW8_002138"
     CDS             5021..5155
                     /locus_tag="AC1LW8_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773943.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5319..6875
                     /locus_tag="AC1LW8_002139"
     CDS             5319..6875
                     /locus_tag="AC1LW8_002139"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIP4773944.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            6868..>6950
                     /locus_tag="AC1LW8_002140"
     CDS             6868..>6950
                     /locus_tag="AC1LW8_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773945.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLG"
BASE COUNT         2673 a         1052 c         1276 g         1949 t
ORIGIN      
        1 atacattaac tataaaaata aatttggaag gaggtaaaat atgaatgaaa atgtaatggt
       61 taaaggttta actgctttaa ctattttaac atctcttggt tttgctgaaa atatttctaa
      121 tcagcctcat tcaattgcca aagcagaaaa gaatgtcaaa gaaattaccg atgcaactaa
      181 ggaaccatac aattcagtgg tagcatttgt gggtggtact ggtgtagttg ttggtaaaaa
      241 tacaatcgta actaacaaac atatcgctaa aagtaatgat atttttaaaa atagagtatc
      301 agcacatcat tcgagtaaag gtaaaggcgg aggaaactac gacgttaaag acattgtaga
      361 atatcccgga aaagaagacc ttgcgatagt tcatgttcat gaaacaagta cagaaggttt
      421 gaattttaat aagaacgtta gttatacaaa atttgcagac ggagcaaaag tgaaagatag
      481 aatttctgtt attggttatc caaagggtgc acaaacaaaa tataaaatgt ttgaatcgac
      541 aggaacgatt aaccatatca gtggaacgtt tatggaattt gatgcgtatg cacaaccagg
      601 taattcagga tctcctgtat tgaattctaa acatgaactg attggtattt tatatgcagg
      661 tagtggaaaa gatgaatctg aaaagaattt cggtgtttat ttcacaccac aattaaaaga
      721 atttattcaa aataatattg aaaaataagt atcatcaatt cattcgtgaa gttgattttt
      781 taaagagtga ttaagaaaac ggttacataa ttaactaaat atactgaatt atgtatctag
      841 atatcaaaat aattaaaaaa gaggaactta aaatgaacaa aaacgtagtc atcaagagtt
      901 tagcagcatt aacaatttta acatctgtaa caggtattgg aataacattg gttgaggaag
      961 tacaacaaac tgccaaagca gaaaataatg tcacaaaagt taaagataca aatatttttc
     1021 catatactgg tgtagttgct tttaaaagtg caactggatt tgtagttgga aagaatacta
     1081 ttttaacaaa taaacatgtg tcgaaaaatt acaaagtggg cgatagaatt actgcacatc
     1141 caaatagtga taaaggtaat ggtggtattt attcgattaa aaagattatt aattatccag
     1201 gtaaagaaga tgtatcagtc attcaagttg aagagcgtgc aatagaacgt ggaccaaaag
     1261 gctttaattt taatgataat gtaacgccat tcaaatatgc ggcaggggct aaagctggtg
     1321 agcgaattaa agtgattggt tatccacacc catacaaaaa taaatatgtt ttatatgagt
     1381 caactggccc tgtgatgtca gtagaaggta gcagtattgt atattcagcg catactgaaa
     1441 gcggaaactc tggatcacct gtattaaaca gcaacaacga attagttggt attcattttg
     1501 cttctgatgt aaaaaatgat gataacagaa atgcatatgg cgtctacttt acaccagaaa
     1561 ttaaaaaatt cattgcagaa aacatagata aataaacaaa ttgactttaa acgagcgttg
     1621 caacatatct cgaattgtaa aggagcttga aaatgaataa aaatatagtc attaaaagca
     1681 tggcagcatt agccattcta acctcagcaa ctggaataaa tgctgcagtc gttgaagaga
     1741 cacaacaaat agcaaatgca gagaagaatg ttacgcaagt taaagataca aatatttttc
     1801 catataatgg cgtcgtttca tttaaagatg cgacaggttt tgtaattgga aaaaatacaa
     1861 ttatcaccaa taaacatgta tcaaaagatt ataaagttgg cgatagaatt actgcccatc
     1921 cagacggtga caaaggaaat ggtggtatat ataaaattaa aagcatttct gattatccgg
     1981 gtgatgaaga catctctgtc atgaatattg aagaacaagc agttgaacgt ggaccaaaag
     2041 gctttaattt taatgaaaat gtccaagcat taaattttgc gaaagatgct aaagttgatg
     2101 acaaaattaa agttattggt tacccattac ctgctcaaaa tagttttaaa cagtttgaat
     2161 ctacaggaac tataaaaaga atcaaagaca atattttaaa ttttgatgca tacattgaac
     2221 ccgggaattc aggatcacca gttctaaatt ctaacaatga ggtcataggt gtggtgtatg
     2281 gcggtattgg aaaaattggt tctgaatata atggtgccgt atactttacg cctcaaatca
     2341 aagattttat tcaaaagcac attgaacaat aaacaaattt aaatatacac catgagcatg
     2401 tgttcaataa ttttaatgaa aaacatcggt cgaatataac atgaaaaaac gtctatatca
     2461 aaagcatcat gaataaacag aggagcacaa aaatgaataa aaatataatc atcaaaagta
     2521 ttgcggcatt gacgatttta acatcaataa ctggtgtcgg cacaacagtg gttgatggta
     2581 ttcaacaaac agccaaagca gaaaatagtg tgaaattaat taccaacacg aatgttgcac
     2641 catacagtgg tgttacatgg atgggcgctg gaacaggatt tgtagttggg aatcatacaa
     2701 tcattaccaa taaacatgtt acttatcaca tgaaagtcgg tgatgaaatc aaagcacatc
     2761 ctaatggttt ttataataac ggtggtggac tttataaagt tactaagatt gtagattatc
     2821 ctggtaaaga agatattgcg gtcgtacaag ttgaagaaaa atcaacgcaa ccaaaaggta
     2881 gaaaattcaa agatttcact agcaaattta atatagcatc agaagctaaa gaaaatgaac
     2941 ctatatcagt cattggttat ccaaatccta atggaaataa actacaaatg tatgaatcaa
     3001 ctggtaaagt actatcagtg aatggaaata tagtgacatc tgatgcggtt gtccaacctg
     3061 gcagctctgg ttcacctata ttaaatagta agcgagaagc aattggtgtt atgtatgcta
     3121 gtgataaacc aacaggtgaa agtacaaggt catttgctgt ttatttctct cctgaaatta
     3181 agaaatttat tgcagataat ttagataaat aaatcatcca tccatacatt gataaatgat
     3241 ttttagaaat taacaacaaa atcaacaatt ttaaatatct ctgtgattct atttattcga
     3301 aatgatttaa aaaataaaac ttcaaaaacc taaccttata tttatacgaa tacttagagg
     3361 agcacaaaaa tgaataaaaa tataatcatc aaaagtattg cagcattgac gattttaaca
     3421 tcagtgactg gcgtcggcac aacagtggtt gagggtattc aacaaacggc taaagctgaa
     3481 cataatgtga aactaatcaa aaatactaat gtagcaccat acaatggtgt cgtttcgata
     3541 ggatctggaa caggtttcat tgtcggtaaa aatacaattg ttaccaacaa gcatgtcgtt
     3601 gcaggtatgg aaattggtgc acatattata gcgcatccca atggtgaata taataatggc
     3661 ggattttata aagttaaaaa aattgtccgt tattcaggtc aagaagatat tgccattcta
     3721 catgtggaag ataaagctgt tcatccaaaa aataggaatt ttaaagatta cacaggcatt
     3781 ttaaaaatag catcagaagc taaagaaaat gaacgcattt caattgttgg ctatccagaa
     3841 ccatatataa ataaatttca aatgtatgag tcaacaggaa aagtgctgtc agttaaaggc
     3901 aacatgatta ttactgatgc tttcgtagaa ccaggcaact caggttcagc tgtatttaac
     3961 agtaaatacg aagttgtagg tgttcacttt ggtggaaacg gccctggaaa taaaagtaca
     4021 aaaggatatg gtgtttattt ctctcctgaa attaagaaat tcattgcaga taacacagat
     4081 aaataaatcc ttacatagat aaatgatttt aaaaattaac aacaaactca acaattcaaa
     4141 tcatctctgt gattccattt attcgaaatg attaaaaaaa ataaaactta aaaaagctaa
     4201 cattataatt atacaaatac ttagaggagc agaaaaatga ataaaaatat aatcatcaaa
     4261 agtattgcag cattgacgat tttaacatca ataactggtg tcggcacaac aatggttgaa
     4321 ggtattcaac aaacagccaa agccgaaaat actgttaaac aaattacaaa tacaaatgtt
     4381 gcaccataca gtggtgttac atggatgggc gctggaacag gatttgtagt tggaaatcat
     4441 acaatcatta ccaataaaca tgttacctat cacatgaaag tcggtgatga aatcaaagca
     4501 catcctaatg gtttttataa taacggtggt ggactttata aagttactaa gattgtagat
     4561 tatcctggta aagaagatat tgcggttgta caagttgaag aaaaatcaac acaaccaaaa
     4621 ggtagaaaat tcaaagattt cactagtaaa tttaatatag catcagaagc taaagaaaat
     4681 gaacctatat cagtcattgg ttatccaaat cctaatggaa ataaactaca aatgtatgaa
     4741 tcaactggta aagtattatc agtgaatggg aatatagtgt catcggatgc aattattcag
     4801 cctggtagct ctggttcacc tatattaaat agtaaacacg aagctattgg tgtaatctat
     4861 gccggtaata agccatcagg tgaaagcaca agaggatttg ctgtttattt ctctcctgaa
     4921 attaagaaat tcattgcaga taatttagat aaataattaa aacttagaca ttcacccaat
     4981 cctgacaaaa tatactataa ctaacattta ttaatatata ttgcattatt taatatgcat
     5041 caaagccaat caacgattga ttttcaccaa ctcaattgtt gattggtttt atttatgtat
     5101 gaatgaacaa ctttttgaca tcattaagaa tataaatgat tttgaaagca tttgaaagct
     5161 acaacatttc tataaaattt ttcaataaca attgcgccac taaaactcaa aatttccacc
     5221 accaacatcc aaattatcaa catcgcaaca taaccaaatg ttataataaa tctattacac
     5281 aaagagataa attacttatg caaaggcgga ggaatcacat gtctattact gaaaaacaac
     5341 gtcagcaaca agctgaatta cataaaaaat tatggtcgat tgcgaatgat ttaagaggga
     5401 acatggatgc gagtgaattc cgtaattaca ttttaggctt gattttctat cgcttcttat
     5461 ctgaaaaagc cgaacaagaa tatgcagatg ccttgtcagg tgaagacatc acgtatcaag
     5521 aagcatgggc agatgaagaa tatcgtgaag acttaaaagc agaattaatt gatcaagtcg
     5581 gttacttcat tgagccacaa gatttattca gtgcgatgat tcgtgaaatt gaaacgcaag
     5641 atttcgatat agaacatcta gcgacggcga ttcgcaaagt tgaaacatct acattaggtg
     5701 aagaaagtga aaatgacttt atcgggctgt tcagcgatat ggatttgagt tcaacgcgac
     5761 taggtaacaa tgtcaaagaa cgtactgctt taatctctaa agtcatggtt aatcttgacg
     5821 acttaccatt cgttcacagt gacatggaaa ttgatatgtt aggtgatgca tatgaattcc
     5881 taatcgggcg ctttgcggcg acagcgggta aaaaagcagg cgagttctat acaccacaac
     5941 aagtatctaa gatactggcg aagattgtca cagacggtaa agataaatta cgtcacgtgt
     6001 acgacccaac atgtggttcc ggttcattgt tgttacgtgt tggtaaagaa acgcaagtgt
     6061 atcgttattt cggacaagaa cgtaacaata ctacatacaa cttagcacgc atgaatatgt
     6121 tattacatga tgtgcgttat gagaacttcg agatccgtaa tgatgacaca ttggaaaatc
     6181 cagccttttt aggcaataca tttgatgcgg ttattgcgaa cccaccatac agtgcgaaat
     6241 ggacagcaga ttcaaaattt gaaaatgatg aacgattcag tggttacggc aagcttgcgc
     6301 caaaatccaa agcagacttt gcctttattc aacacatggt acattaccta gacgatgaag
     6361 gtaccatggc agtcgtactc ccacatggtg tattattccg tggtgccgca gaaggtgtga
     6421 ttcgtcgcta tttaatagaa gaaaagaact acttagaagc cgtgattggg ttaccagcca
     6481 atattttcta tgggacaagt attccaacat gtattttagt atttaaaaaa tgtcgccaac
     6541 aagacgacaa cgtactattt atcgatgcat ccaatgattt tgaaaaagga aaaaatcaaa
     6601 accatttaag cgatgcccaa gtcgaacgta ttatagacac atataagcgt aaagcaacga
     6661 ttgataaata tagctacagc gcgacattac aagagatcgc cgataacgat tacaacttaa
     6721 acataccgcg atatgttgat acattcgaag aagaagcacc aattgattta gatcaagtcc
     6781 aacaagattt gaaaaatatc gacaaagaaa tcgcagaaat tgaacaagaa atcaatgcat
     6841 acctgaaaga acttggggtg ttgaaagatg agtaatacac aaacgaaaaa tgtgccagag
     6901 ttgagattcc caggatttga gggcgaatgg gaagagaaga agttagggaa
//
LOCUS       DBNENW010000129         6930 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNENW010000129 DBNENW010000000
VERSION     DBNENW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.130"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            273..1466
                     /gene="fmtA"
                     /locus_tag="AC1LW8_002141"
     CDS             273..1466
                     /gene="fmtA"
                     /locus_tag="AC1LW8_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HIP4773946.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(2003..2281)
                     /gene="qoxD"
                     /locus_tag="AC1LW8_002142"
     CDS             complement(2003..2281)
                     /gene="qoxD"
                     /locus_tag="AC1LW8_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HIP4773947.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2290..2895)
                     /gene="qoxC"
                     /locus_tag="AC1LW8_002143"
     CDS             complement(2290..2895)
                     /gene="qoxC"
                     /locus_tag="AC1LW8_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HIP4773948.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2885..4873)
                     /gene="qoxB"
                     /locus_tag="AC1LW8_002144"
     CDS             complement(2885..4873)
                     /gene="qoxB"
                     /locus_tag="AC1LW8_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HIP4773949.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4873..5973)
                     /gene="qoxA"
                     /locus_tag="AC1LW8_002145"
     CDS             complement(4873..5973)
                     /gene="qoxA"
                     /locus_tag="AC1LW8_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HIP4773950.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6275..6397
                     /locus_tag="AC1LW8_002146"
     CDS             6275..6397
                     /locus_tag="AC1LW8_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773951.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6545..6862)
                     /locus_tag="AC1LW8_002147"
     CDS             complement(6545..6862)
                     /locus_tag="AC1LW8_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HIP4773952.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2478 a         1070 c         1178 g         2204 t
ORIGIN      
        1 aatcaaaaag catttttaaa aatataatgc gactttagta tgatgtctta aaaaatttaa
       61 aggcgtaata taaattcgag tgatttatac aaagtgataa ggtataatta attttgttgt
      121 atagataaca tattagaaaa acttattact atgtagcgta aagcataaat gaatgataag
      181 ttaatttttg taagttgtat tattaggcat ctaggttcaa atttttaacg atttaaatat
      241 ataaaatgaa tacattgtga ggcaattatc aaatgaaatt taataaagta aaactagtta
      301 tacatgcgtg tgtactatta tttatcatta tttctatagc gttaattttt catcgattac
      361 agacgaagac acattctata gacccaatac ataaggaaac aaaattatca gacaatgaaa
      421 aatatttagt ggatcgtaat aaggaaaagg ttgcgccgtc taaactaaaa gaggtatata
      481 atagcaagga tcctaaatat aagaaaattg acaagtattt acaaagttca ttatttaacg
      541 gttcagtagc tatatatgaa aatggcaaat tgaaaatgag taaaggttat ggatatcaag
      601 attttgaaaa aggtattaaa aacacaccga atacgatgtt tttaataggt tcagctcaaa
      661 aattttcaac agggttactg ttaaaacagt tagaagaaga acataaaata aatatcaatg
      721 atccagtaag taaatacctt ccatggttta aaacatctaa gcctatccca ttgaaagatt
      781 taatgttgca tcaaagtgga ttatataaat ataaatcctc aaaagattat aaaaatttag
      841 atcaagcagt taaagcgatt caaaaacgtg gtattgatcc taagaaatac aaaaagcata
      901 tgtataacga tgggaattat ttagtacttg cgaaagtaat tgaagaagtt acaggtaaat
      961 cttatgctga gaattattat acaaaaatag gagatccttt aaaacttcag cacacagcat
     1021 tttatgatga acaacctttt aaaaaatatc tagcaaaagg ttatgcttat aatagtacag
     1081 gactttcatt cttaagacct aatattttgg accaatacta tggtgcaggt aatttatata
     1141 tgacaccaac agatatgggt aaattaatta ctcaaataca acaatataaa ttattcagtc
     1201 ctaaaataac caatccatta ttacatgagt ttggtacgaa acagtatcca gatgaatatc
     1261 gatatggttt ctatgctaag ccaacattga atagacttaa cgggggattc tttggacaag
     1321 tctttactgt ttactataat gataagtatg tagttgtact tgcattaaat gtaaaaggaa
     1381 acaatgaagt tcgaatcaaa catatttata atgatatttt aaaacaaaat aaaccttaca
     1441 atacgaaggg tgttattgtt caataattaa tatagaagat ataacatgta tatggcatta
     1501 aggcatcgac cttatctgac cagtatacga gttatatctt cttttttata gtggtaaaaa
     1561 gtttaaagta taaggttgaa gaaggatgag tttaaaaata tgtgttaact gataaaaggg
     1621 gaaatcattt ggtgagttgg catcagacta aaatgaatga agacgaatac gttggtccat
     1681 gcgtggtgtg aatgttttct aaatctaact ggaaatgata atttaataat gtacactttt
     1741 gattgtttaa acatgtacaa tttttaaaaa cctagggata attttattga cgtgaacaac
     1801 attgagatta tctttagaca taaatatgga ttttgaagtt actatattta ttagttgtgg
     1861 ttgttaatgt attaattgaa ttgattataa tgattgtctt ataagatata gaaggggact
     1921 taaccattta aaaattgaag gaaaataaaa accagctaac aaaagtgtta gctggttaaa
     1981 ctttacatta ttaaaatgtt gtttataagt gtgaagagtg accgccttgc ataacccaat
     2041 atgttccgac aacgaaacaa agtgtaatta caagagcaaa gataactttg aatgtttgta
     2101 aacgtccatc tttaccttca gttaaatgca tgaacattaa taattgaagt cctgcttgga
     2161 cgaatgcaaa gccaaagata attgtcaact tcgcgtggaa tgttaatgac gtgtatagtg
     2221 ttacgtatac tgctaaaagc gttaatacga tagatgcgat aaatcctaca gtatgtttca
     2281 ttattgtact catccgctat acaccatccc tatcatatat acggcagtaa agatgaaaac
     2341 ccaaacaaca tctaagaagt gccagtataa acttactata aataattttg gcgcattata
     2401 tttgtctaat ccgcgtcgtt ggatttggat taataaacaa atggcccaaa cgatacctag
     2461 cgatacgtga caaccatgcg ttcctaatag gataaagaaa ctagaccagt aagaaccaat
     2521 tgttgggtta acgccttctg atgcatagtg tgcgaattca taaatttcga atccaacaaa
     2581 gactaaacct aaaagtaacg taatgatcat ccaaaacatc attaactttt gtttttcttg
     2641 gcgcatgtag taaatagcaa taccacatgt gtaagaactg aataataatg caaacgtcat
     2701 tattaaaaca agaggcaatt caaataactc agtagtcatt ttacctgcat aatcgccacc
     2761 atgttgcaaa gttaatagtg tcgcaaatag ggtaccgaat aacgcaaatt cggctgtaat
     2821 gaaaatccaa aagccaagct tatttaattc gccttcatgt gtgcgtgaat caatagtgtt
     2881 tgtatcatga ctcatgactt acagcctccc tttctttaat acgcgcttct cttaatctag
     2941 cttcagtttc agcaacttca gcagcaggga tatgatatcc gtgatcgatt tggaaactgc
     3001 gataaatcat agtaccaaaa ataccgaata aacaaattaa tgctggaatt acagtttcga
     3061 aaattaagaa gaaaccgccg atagtcataa agataccaat ccagaatcca acaggagtat
     3121 tgtttggcat atgaatgtct ttgtaattat ggttgtctaa gtaatgacga ccatgttctt
     3181 tcatatcaac aaatgtgtcg tagtcattcc aatctggtgt aatggcaaag ttgtatttag
     3241 gtggaattgc tgatgcagta gtccactcta gagtacgacc aaggccatcc cagttatctc
     3301 cagttgcttc acgtggagat ttgaagtgac tgtatacgat actaacaaca aggaataaga
     3361 atccgattgc cattaataat gcaccgatag ttgaaattaa gtttaataag aaccaaccat
     3421 ctgatggcat ataagtgtat aaacgacgtg gcataccatc taatccaaga atgaattgtg
     3481 gtaagaaaca aacgttaaat ccgatcatga agaaccagaa gcaccatttg tttaatgttt
     3541 cgtttaattt gtaacccatc atctttggat accagaagat taaaccagct aagcaggcaa
     3601 atacaacacc agtaaccaat gtatagtgga agtgagctac taagaagtac gtattgtgat
     3661 attgatagtc agctgatgcc attgctaaca ttacacccgt aacaccacct aaaaggaagt
     3721 tagggataaa tgctaatgag aatagcattg gtgactcaaa tgtaatacgt cctttatata
     3781 atgttaataa ccagttaaac aatttcacac cagttggaat accaatcagc attgttgaaa
     3841 ttgagaagaa tgagttgatt aacgcaccat tacccattgt gaagaaatgg tgaacccaaa
     3901 ctaagaaact aaggaacgcg ataccggcag ttgcccatac catactttga tgtccgaata
     3961 aacgcttacg agcgaatgtc gggataattt ctgagtaaat accaaatgct ggaaggataa
     4021 cgatataaac ttcagggtgc ccccataccc agaagaagtt agcccaaagc attggcatac
     4081 cgccatgtgc aactgtgaag aatgctgtgt caaatattct atcagttgtc attaatgcta
     4141 acgctactgt taaaggaggg aaagcaagaa taacaattaa tgtagtaata aatgttgtta
     4201 ctgtaaacat tggcatttgc ataaacttca tagttggtgt tttacatctt aaaattgtta
     4261 caaagaagtt gatacctgta gctaaggtac caagccctga aatttgtata gctattaagt
     4321 aatagttaac acccggacca ggactgaatt cacctgctag tggcgcatag tttgtccaac
     4381 cagctgctgg tgaaccacca ataataaatg acaggttgaa taaaatcata cctgcaaaga
     4441 atagccagaa acttacgttg tttaatactg ggaatgcaac atcacgtgct ccaatttgta
     4501 atggaacaac gatattccat aaaccaaaga taaatggcat tgccatgaag ataatcatga
     4561 ttacaccatg tgtactaaaa atttcgttat agtggttaga ttctaaaaat ttgttatcag
     4621 gtactgttaa ttgcgcacga ataagtaacg catcaatacc accacggacg aacattaata
     4681 cggcacagat taaatacata ataccgattt tcttatggtc tacagatgtg aaccattctt
     4741 tgtaaagata tttccataat ttaaagtaag taattactgc gattaaacca ataactaaga
     4801 atggggcacc aatttgtgcc attgtaatca tccagttacc tttaactagt aattgatccc
     4861 atggaaaatt cattaatgtc cacctccatg atcatcattg tcttgatctt gcgcatcttt
     4921 tgaaattttc ttcatttctt tcgcattttt cgattcatct ttcttgaact cattgttata
     4981 tggttcgtca tttccaagaa tcatcaactt cataccatgt cgtttatagt tcgcatttgt
     5041 aatttgagct ttacgagcag gtattaatgg tttgtctgat acatctttaa acatattttc
     5101 ttcactagtg aagtttggat ctttcaattc gaaattgaaa cgtttatatg catagaagat
     5161 gtattctgga tcggctgctg gatcaacaaa cgccatatgt gtaccattaa attctaaagc
     5221 tttattaggt gtgcttggta ataattgttt atcaaatgta tcttgatcta acgttttctt
     5281 acctttaact tctttcaccc atttgtcgta gtctttttga ctaacggcat ttactttaaa
     5341 tgtttgacgt gagaatcctt caccattgaa gttagagtta cgacctctga acgtaccagt
     5401 ttgagatgct tctaacgtcc aattcattgt catgccagtc atggcatatt tttgaccacc
     5461 taattgtgga atccagaaac ttgtcattgt atccatagct tgaagcttaa atacaacagg
     5521 acgatcttta gggattgtta atgtgttaac agtctctata tgttcatctg gataagcaaa
     5581 gaaccatttg tatcctgcac ttactgcata tacaaccatt ggatctttct cactcttcgg
     5641 tggtttttcg taatcgtata aagttttaac tgtaggaata gctaaagcag caacgattat
     5701 gataggtatt acaaaccata ttgtttcaat gatggcatta tggtgcatct taccagattc
     5761 ggcattctta ttataactat acttgtaaat aaaaatggcg aacatgccaa gtacaacgaa
     5821 acaaataaca agcatgaaga cgattgaata aagaatcaag aacttctgac tacttgctac
     5881 tggccctttt gcgttgaaaa tttctatatt tgaacaacca ctaagtaaaa ttagtgtgcc
     5941 aaataataga agcaaagact taaattttga cacttttttg acctcctaat actacaaatg
     6001 tagggcttaa cattaatttt aagttattac acaatattta caagggctta tgggaaaaaa
     6061 attaataaaa ttgtatcaaa aatgttgata aatcaaggtg tgacgtgggt tcacacattt
     6121 gttaaaatta tgtgtacatt ttgtgactaa tagcgttttt gaaaaaattg cttcatattt
     6181 aaaaggaaag ttgagaatag ctttcaatta aaggttaatt gaatgaaaat aaaaaaagtt
     6241 tgaaattgtc taataaattg aaaatgatgg ctagttgatt gaaaggaagc gcaaacattt
     6301 taataaggaa tttgccaaaa gtaatttatc gttaaacaat gaaaagtgga aatttgtttg
     6361 ttcaaaatca aaacattttt ttaaaaagag cagttaagtt ttaataattt gataggtagt
     6421 cctttggtat acagaaaaaa tacaatattt gggttaaagt tgcagatgaa gttagaataa
     6481 caaataaaat ggtagcaaaa catgttcgtt tgctaccatt ttatttgcag tatagtgatg
     6541 agggctattt aacctcaata tacctagtgg aaattttaat tgcaccatct gaatcagtga
     6601 catgatattt aatttgatat ttaccagatt tagatgtatc aatttggcca tcgactttaa
     6661 ttttatcggt taaatctcca tcttctttat caaatgcact tatgccgttt agaaggttat
     6721 aatcttgacc tttcttaata acgatatcat tagcgccttt aatttgtggt gtagtattcg
     6781 tcgttgcatc tgcatttaaa tttggtgtta ctagtgtagc agaaacaccg agggctgata
     6841 atgactgtaa tagtttattc ataatgtaac ctccgaatga taagtcattt ctgagttagc
     6901 tttactatac cttaatttat attatttaca
//
LOCUS       DBNENW010000130         6917 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNENW010000130 DBNENW010000000
VERSION     DBNENW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.131"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            9..428
                     /locus_tag="AC1LW8_002148"
     CDS             9..428
                     /locus_tag="AC1LW8_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIP4773953.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            455..565
                     /locus_tag="AC1LW8_002149"
     CDS             455..565
                     /locus_tag="AC1LW8_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773954.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(635..4405)
                     /gene="atl"
                     /locus_tag="AC1LW8_002150"
     CDS             complement(635..4405)
                     /gene="atl"
                     /locus_tag="AC1LW8_002150"
                     /EC_number="3.2.1.96"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HIP4773955.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKAAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDIDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4633..5067)
                     /locus_tag="AC1LW8_002151"
     CDS             complement(4633..5067)
                     /locus_tag="AC1LW8_002151"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4773956.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5222..5692)
                     /locus_tag="AC1LW8_002152"
     CDS             complement(5222..5692)
                     /locus_tag="AC1LW8_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HIP4773957.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5740..>6917)
                     /locus_tag="AC1LW8_002153"
     CDS             complement(5740..>6917)
                     /locus_tag="AC1LW8_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HIP4773958.1"
                     /translation="VLIVVPIVFATLLFKTSQDAFESSQDSKNANRQSNLRDNKVNPE
                     EQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRMLSIPRDTISYIP
                     KVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEAVNELGGIYYDVP
                     YDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQRQMELIKILFQKA
                     QEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQLKGKDDMLNGI
                     YYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPLTPLDSSLLRKE
                     QNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQAPQASNNQNGVVN"
BASE COUNT         2010 a         1139 c         1170 g         2598 t
ORIGIN      
        1 agaacaacat gtataaacaa cttgaaaaac ttattacact gactaacaat gacttaaact
       61 tagtgaatag aagatttgga caacgcacgg atatcacatc tgaacagcta gaacttctcc
      121 gtattttatt taattacgat cgcttatcac agtatgattt gacgatgaag attagcaggg
      181 aacaatctat agtttcaagg tggattaaga aattagtttt gaaagggtac atcacaagtc
      241 aacaatctag cgaagattta agatgtaaag aattaatttt gactgatcaa gcacgtacat
      301 taatttcaca aataaataat gcacgttgcg aattgattga agcaagatgt caatgtttat
      361 cggaagtcga attagacaat ttaaatcaat tacttgataa gttaaatcaa cgacgcatat
      421 cgttgtaaca cgacaatagc aaacacataa tttagtgtcg ttgtgcacac aacaaaaatt
      481 gaatcatgaa ttacaagcaa aagtagcggt gattgttaaa attgatggta aacaatcacc
      541 gctatttttg cttgtgtatg tataaaaaag ggatcaaagg tcatccccca tgattgatag
      601 tgggggatga cttttgatcc tatgttcatg ttgcttattt atattgtggg atgtcgaagt
      661 atttgccgac ttcgccaatt ttatcatagt agcctttgat gattttagca ttgatgttag
      721 cccaatctat atctgtagca tattggtgtg ttcctggatg tgcaggattc catctcattt
      781 tgtaaagtgt attttgacca gcttttacat atgagttgcc gatgaattta gcaccaccaa
      841 cgattgcttt tgatactgtg tcccaaccag cttgtttagc atatttaata ccttcacgta
      901 aaggatcgtt atcatatgca gcaataccaa atacgttatg gtatttcgtg tttgagttag
      961 ttacaacttt gttgttcact acatctgcac ctttcgctaa ttgagaagta ccgttacctg
     1021 tttctaatag ggcatgtgag ataagataaa cttcattaat gccatacatt tgagcagctt
     1081 tgttaaatgc agcaccttgg ttttctaata cacctttacc ttttaagaat tgattgattt
     1141 tatcaataga aatattttgt ggttggtcta agcgtaagaa ttgatatttt aatgctggat
     1201 cttgagctaa acgcttcgta tccattgcat gcttaacatc attaaagtta gcatctgtcc
     1261 acttacctgg tacacgttgt acttgtggtt tatattgtaa accagcttgt atttgagcaa
     1321 cttggtttaa tgtcatacct gtttgattat acttaattaa ttctttagct aaatcagttg
     1381 atttaatcca tgctaattta ccgttagata atttaccata gtaccaagtt tgtccattaa
     1441 tgacttgttc tttaacaact gcgaatggtt gttcattaaa tgcttttaat gagtatttag
     1501 ctgtatcaga atttggtgtt acatagtaat aaccattacc atttttaatt acataagtgt
     1561 agttataatc tttggcagct gatgtagttg gtttaacagc agttggtgca gttaaatctt
     1621 ttgcatttac ccaaccagtg cggttattaa tagtaccgta taaataaaca tctttgccta
     1681 cagatacttg tttcgttgca ttaaatgtac cttgagcaat gttattgcct gttaaaatga
     1741 cttggttttt agtaccccaa ggaaccattg ataagccgtt atttgattta ttaacagtat
     1801 atttttgagt cgttttaact tctttgccta agttttgaac atttaagtct tttacattga
     1861 accaacctaa tgggatgtta tggcttgtat tgtttaataa tacatacgtt tcattaccat
     1921 gagcacgctc ttttgttaca tagaacgtac ggtctgcata tttcgcaccg tttttcgctg
     1981 ttttttcata aacagaagca cgaataccag tgttgtttgg tttaacttga gcaatcttgc
     2041 taactgtttg agtcgtttgt ggtttagtaa cagtataagc ttttacagct gtttttggtt
     2101 gtgctactgc ttttttaggt gcagcaggta cagctaaata tgctttactt acccaaccag
     2161 atttaccatt tacagttcca aataaataga tagatttatc aatttgttgt tgcttagtcg
     2221 ctttaaaagt ttggttacct gtaccagaaa ctgcaccagc ttcttgttta taagtgcccc
     2281 aaggtactga atataattta gtgcctggtt ttactgtata tgtttgcatt acatttacag
     2341 gtgattttgc attgttataa ataacgtcac cttgtttaac ccaaccaatt aaagttggac
     2401 tattgtaatc tttaactaag tagaatttgt ttccacctaa acttgcttct tttgttacag
     2461 caaatgtttt ttgaacttct ttcgttggct taccagtttt gtcataaact gtagtgaata
     2521 agccattgtt tttagcatta atttgagcaa caccgtttaa tgatgaaact gttaatttat
     2581 tatttgttgt aggtgttgat ggcttaggtg ttggtgtagg cgtaggttta gcagtatcaa
     2641 ctaaatatgc tttacttacc caaccagatt taccattcac agagccatat aaataaattg
     2701 atttatcaat ttgttgttgc tttgaagcct taaatgtttg gtttccagag ccagacacac
     2761 taccagcaac ttgtttagat gtaccccaag gtactgtata aagtttcgta ccaggtttga
     2821 ttgaatatga ttgatttaca tttacaggtg atttagctgt gttgtaaacc acatcgcctt
     2881 ctttaaccca accaaattta ttaccagaat tgtaatcttg aacaagatag aatttttgat
     2941 tacctaatgt agctgtttta gatacagcaa atgttttttg aacttcatta gttgctttac
     3001 cagttttgtc gtatacagta gtatataaac cactatttgt tggtttgatt tgtgcgacac
     3061 cattgtttgc agcaactgtt aatttaccag ttgatggttt cgacggtgtt gttggttttg
     3121 atggtgtagt aggggtagtt gtagattgcg taccccatgg cgccacttta cccattttta
     3181 ttaaatattt ttcattaatt aagtcatata attgatcata actataatta tgacttctta
     3241 aatatccatg tggatcggca tggtcagtac cacctaaata tttacttaca gcgtagtgag
     3301 tccatactgt accatttcca tcatactcag cactgtctgg ttttaaacca taatattgta
     3361 attgtgtagc tgcatagtca gcatagttat tcattgaacg tgcaaatgaa gcatagtcgt
     3421 gtgtgtgtac gatttcaaca ttgatgaatc tagggttacc gactgcaccg acaccccaag
     3481 ataagtaatc cgttggtgct gtttcgatta tacgatcccc atcaacaaat gcatgtacga
     3541 atgcgttttg atagttattt ttcatataac taatttcacc atttatcgtc gaacgatcat
     3601 tagctgtatc atgaactacg ataccttcag gacgacctac gccgttacgg tatgcgtatt
     3661 tagggaagta agatgtataa tcttcttcaa ttttaggtgc ttttaagtta tttttacgaa
     3721 tgtaatcgtt aattgaagag tttacttgtg gtttatattt tggcaaactc gtttttggtg
     3781 ttgcagcaac tgatcttggt tgtgctgaag cgctaaaagt agttacttta ggtgtcgctt
     3841 cagttttagc tttaggtgct gatgtagttg cagctttagg tgctgcggct ttatattgcg
     3901 tttcaagagc tgcaggttta gcagctgatt taattaattc tggattaatt tgattttctg
     3961 aattatcatc ttcatcatca actaaactat aaccagcatt tgtaacatta gtgttagttt
     4021 taggtgctgt agtgcttgtt gactttgcaa caggctgcgt attatttgta gtcgctgatt
     4081 gattagcacg agtgtcacca tttacttgtg cagtatcaac tttttgactt acttgagcat
     4141 tgcctgtttt gttatttgct gtttttggtt ggacaatagc agggtcttga tatacttgag
     4201 tgccagaaat gttttgcgtt ggatttttta cctcagcttt tgcttgttca gtagttgctt
     4261 taactttatt actatctaaa acgtttttat tagtagtttg atcttgtgtc gtctcagctg
     4321 cttgaacttg atgtgcagtg actgctgaac ctacaagcgt taatgcaacc attgatggta
     4381 gtttgtaatt gaattttttc gccattctat ttattactcc taacatttat taattattac
     4441 taacattata gtacctgttt tatatacctg tgcgtattta accaatttgt aatatactct
     4501 taatttacat tttcactgta aaaaaatgaa aaaaattttg aatctcaatg gtgacgtgac
     4561 aaatcaaata gaattgatgt caatttaaga attggtttat tacaaatgtt tctaaaatag
     4621 atacaacttt ttttaattaa gcgaggttaa cttttttgtc atttcaatat gctcgatgcc
     4681 ttcctcaaga aatatattac ctctcatttt aaagtttaaa ctttcataaa atgggatagc
     4741 atgacattgg gcattcatag tagctacgta aaaaccttca tctttagcta atgattctac
     4801 agcttgcata agcattctac ccattccttg tccacgatgt gatttcatca cagctactcg
     4861 ttctattttg acagttgttt cattaatagg gcgtattcga gcagtggcaa ctggctgtcc
     4921 attatcatat ccaatgaggt gaatagattc agattcatat tcatcaattt cactttcctc
     4981 agggacgcct tgttcttcta caaacacttt ctttcttata tagaagcaat cttctaacat
     5041 cttttgattg tttacttttg aaaacataaa attcctccac atcctaacaa aatcatttac
     5101 atttaattta ctaataataa catagaataa cgattgttat tttcctcaaa aataattaga
     5161 cataaaaaag ctataaagat gattgttgtg aatcatcttt atagctaatt aattattaat
     5221 gttatagtgc ttcttttcta ataattgctg tatattgcca aaagattcgc atttgagcaa
     5281 tattccaatt attcattccc attgtaaatc ctgaaggctt aaatatattc acatcagtta
     5341 cttctaaagc tgctgcaact aaatattgta atggaatgct aagtgatttc attgtttcgg
     5401 taatgccatt ttcatcatat acccatgaaa atggtaactc tgattcgaaa acatcaacac
     5461 tattgaaaat ttcaggtagg gctaaaatat aacatgcacc atctacaata ggattgtcga
     5521 tactattttt gtaataaatt aggagtgctt catagttttc tcgatgctca tgattgacgt
     5581 aaaaaaattc acttctaaaa tgagccatat cttggctatg aatgatttgt tgttctaaca
     5641 tattaaacat gccatttata tttgcaatct tttcgtatgt ttttctactc atgtatgatg
     5701 ctcctttcat aaatagtaaa tgttaatcgt catataatat taatttacaa caccattttg
     5761 gttatttgaa gcttgtggcg cttgttgtgt gccaccttga ttttgattta agttttgatc
     5821 tgtagcaggt tgttgttgat tgctggaatc actgttatta gttgaatcat tgttctcgtt
     5881 agatgtctta tctttatctg tcgtatcatt ttgttctttt ctcaataaac tactatctaa
     5941 aggcgttaat ggtattaatg aaccataatg attaatgaca cgttgatcta agaaatcatt
     6001 tttatcatta ataggtgata attctaagtc tttacgaagt aagtttgcat atttttgaat
     6061 gctttcaaca cttggatgat aatagtaaat accatttaac atatcatctt taccttttaa
     6121 ttgcgcagtt ttaatttcaa catcatttgt taagtacatt tttgctaaag ctttaatttc
     6181 agagttagtt aaattatgct ttgcattttt acctacaatt tgaatcacgt tatcaagttt
     6241 atcaatagaa tcaacttcct gtgctttttg gaataaaatc ttaattaatt ccatttgacg
     6301 ttgtccacgt tttaagtctg aatcatgatg tctagttcta gcaactgcta aagcctcatc
     6361 accatttaat ttttggtacc cttttttaat tttaatctta ccagtatcat ctgtgttagg
     6421 ttcatttaag tcgtatggca catcatagta tatgccacca agctcattta cagcctcgac
     6481 aaatgctttc atattgactc tcacataata atcaacaggt acattcatgg tagcttctac
     6541 cgaatccatt gcggcaattg gaccaccata tgcatgtgca tgggtaatct tatcgtaata
     6601 gccaacttta ggaatgtagc tgatagtatc acgtggaata ctaagcattc taatttgatg
     6661 ttttgattga ttaaaagtag ttaaaatcat agcgtctgat ctagagtgtt cagcatcctg
     6721 tccttttttt cttcttccat cgttatcatc gatacctaag aaaagaatag agataggttg
     6781 ttcttcggga ttgactttat tatctcttaa gttggattga cgattagcat ttttgctgtc
     6841 ttgagaagat tcgaatgcat cttgggacgt tttaaaaagt aacgtagcga agactattgg
     6901 aacaacaatg agaacca
//
LOCUS       DBNENW010000131         6878 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNENW010000131 DBNENW010000000
VERSION     DBNENW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.132"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            220..1203
                     /locus_tag="AC1LW8_002154"
     CDS             220..1203
                     /locus_tag="AC1LW8_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIP4773959.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1394..2320
                     /gene="pstC"
                     /locus_tag="AC1LW8_002155"
     CDS             1394..2320
                     /gene="pstC"
                     /locus_tag="AC1LW8_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIP4773960.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2322..3239
                     /gene="pstA"
                     /locus_tag="AC1LW8_002156"
     CDS             2322..3239
                     /gene="pstA"
                     /locus_tag="AC1LW8_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIP4773961.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGILGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3286..4137
                     /gene="pstB"
                     /locus_tag="AC1LW8_002157"
     CDS             3286..4137
                     /gene="pstB"
                     /locus_tag="AC1LW8_002157"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIP4773962.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYILSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4144..4785
                     /gene="phoU"
                     /locus_tag="AC1LW8_002158"
     CDS             4144..4785
                     /gene="phoU"
                     /locus_tag="AC1LW8_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIP4773963.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4924..6738)
                     /gene="pepF"
                     /locus_tag="AC1LW8_002159"
     CDS             complement(4924..6738)
                     /gene="pepF"
                     /locus_tag="AC1LW8_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP4773964.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKEPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2394 a         1078 c         1170 g         2236 t
ORIGIN      
        1 ttgtgtacat atttaaattt cgataatttt tcaggaagca ttttaatttt actaatgaag
       61 caatattttt tagattaaca aaaattaata tttacatttt cttaacaaat ttttatgtaa
      121 catttacagt ttctaaaaat gaggttaata attcaaggtt aagataaaga tgtaatcaat
      181 acaaatacta tttgttgttc atacagggag gatatttcaa tgaaaaaatg gcaatttgtt
      241 ggtactacag ctttaggtgc aacactatta ttaggtgctt gtggtggcgg taatggtggc
      301 agtggtaata gtgatttaaa aggggaagct aaaggtgatg gctcatcaac agtagcacca
      361 attgtggaga aattaaatga aaaatgggct caagatcact cggatgctaa aatctcagca
      421 ggacaagctg gtacaggtgc tggtttccaa aaattcattg caggagatat cgacttcgct
      481 gatgcttcta gaccaattaa agatgaagag aagcaaaaat tacaagataa gaatatcaaa
      541 tacaaagaat ttaaaattgc gcaagatggt gtaacggttg ctgtaaataa agaaaatgat
      601 tttgtagatg aattagacaa acagcaatta aaagcaattt attctggaaa agctaaaaca
      661 tggaaagatg ttaatagtaa atggccagat aaaaaaataa atgctgtatc accaaactca
      721 agtcatggta cttatgactt ctttgaaaat gaagtaatga ataaagaaga tattaaagca
      781 gaaaaaaatg ctgatacaaa tgctatcgtt tcttctgtaa cgaaaaacaa agagggaatc
      841 ggatactttg gatataactt ctacgtacaa aataaagata aattaaaaga agttaaaatc
      901 aaagatgaaa atggtaaagc aacagagcct acgaaaaaaa caattcaaga taactcttat
      961 gcattaagta gaccattatt catttatgta aatgaaaaag cattgaaaga taataaagta
     1021 atgtcagaat ttatcaaatt cgtcttagaa gataaaggta aagcagctga agaagctgga
     1081 tatgtagcag caccagagaa aacatacaaa tcacaattag atgatttaaa agcatttatt
     1141 gataaaaatc aaaaatcaga cgacaagaaa tctgatgata aaaagtctga agacaaaaaa
     1201 taataagacg caatttcaaa tgtgtcttga aacatgattt tgatggtgaa tcattattta
     1261 gagtacaaag cttgatttat cgagacgctg attttgacat tcagttagtc tacaagctta
     1321 tcaacttaaa atagtggttc atcattattt tacaaatcta attattttgg gagtaataga
     1381 aagaggtttg attatgactt catctactaa tgttaaagct ttaatcgaaa aaaataataa
     1441 taaaaaaggg aagcataatg acaaaattat accagttatt ttagccgcaa tttcagcgat
     1501 ttccatttta acaacactag gtatattaat cacattgctt ttagaaacca tcactttttt
     1561 caccagaatt ccaataactg aatttctatt ttctactact tggaatccta ccggttcaga
     1621 ccctaagttt ggtatctggg cattgataat agggacttta aaaatcacag ttattgcgac
     1681 tatatttgca gttccagtcg ggcttggtgc agcaatttat ttaagtgaat atgcaagtga
     1741 ccgtgcacga cgcataatta aaccgatatt agaaatttta gcaggaatac caacaattgt
     1801 gtttggtttc tttgcattaa cctttgttac accagtatta agatctttca taccaggtct
     1861 tggagagttt aatgctataa gtcccggctt agttgtcggt attatgattg tccctctcat
     1921 cacaagtttg agtgaggatg caatggcatc tgtaccaaat aaaattcgag aaggtgccta
     1981 tggacttgga gcaactaaat tagaagtagc aactaaagtc gtacttcccg cagcaacatc
     2041 aggtattgta gcttcaatcg ttctcgcgat ttcaagagca attggagaaa cgatgattgt
     2101 atcattagcg gcaggtagtt cgccaacagc ttcattaagt ttaacaagtt cgattcaaac
     2161 aatgactgga tatattgttg agatagcgac aggtgatgca acatttggat caaatattta
     2221 ttacagtatt tatgctgtag ggttcacact atttatcttt accttaatca tgaatttact
     2281 ttctcagtgg atttctaagc gttttaggga ggagtattaa tatggaaacg acagataata
     2341 atagacaatc actcgtcgat caacaacttg tccaaaaaca tttatcatcc agaacggtta
     2401 aaaataaagt gttcaaactc atatttttag catgtacatt attaggactt gtcgtactta
     2461 ttgcgttgtt aactcaaaca ttgattaaag gggtaagtca tttaaattta cagtttttca
     2521 ctaatttttc ttcttcaaca ccatctatgg ctggcgttaa aggcgcgtta atcggttcac
     2581 tttggttaat gttaagtatc attccattat caatcatcct aggaataggt acagctatat
     2641 acttagaaga atatgcgaaa aacaacaaat ttactcagtt tgttaaaatc agtatttcca
     2701 atttagctgg tgtaccatca gttgtatttg ggatattagg ttacactttg ttcgttggtg
     2761 gtgcggggat tgaagccttg aaaatgggta acagtatatt ggcagcagcg ctaacaatga
     2821 ccttactgat attaccaatt attattgttt caagtcagga agcaattaga gctgtaccta
     2881 actcagtacg cgaagcttct tatggtttag gtgctaataa atggcaaacg ataagacgtg
     2941 ttgtcttacc agcagcgtta cctggtattt taactggatt cattttgtct ctttcaagag
     3001 cactgggaga aacagcgcca cttgtgctaa tcggtatacc gactatatta ttggcaacac
     3061 ctagaagtat attggatcaa ttttcagcat taccaatcca aatatttact tgggcgaaaa
     3121 tgcctcaaga agaattccag aatgttgcat cggcaggcat tatcgtttta ctagttatct
     3181 taatcttaat gaatggcgtt gcgattattt tacgtaacaa atttagtaaa aaattctaat
     3241 ttaaacaatc aatctcattt atctattaaa aagggagttt taaatatggc gcaaacactt
     3301 gcacaaacta aacaaatatc tcaaagtcat acgtttgatg tctcacaaag tcatcataaa
     3361 acaccagatg atacaaactc acattctgtt atatattcaa cacaaaattt agacttatgg
     3421 tatggcgaaa atcatgcatt acaaaatatt aatttagata tttatgaaaa ccaaattact
     3481 gccattatag gtccatctgg ttgtggcaaa tcaacatata ttaaaacttt gaatcgaatg
     3541 gttgagttag taccttctgt gaaaacagct ggtaaaatat tatatcgaga tcaagacatt
     3601 tttgatcaaa aatattctaa agaacaatta cgtacaaatg tgggcatggt ctttcaacaa
     3661 cctaatccat ttccaaaatc aatatacgat aatattactt acggtccaaa gattcacggt
     3721 attaaaaata aaaaagttct tgatgaaatc gttgagaaat cattacgtgg cgctgcaatt
     3781 tgggatgaat taaaggatag gttgcacaca aatgcatata ttttatccgg tgggcaacaa
     3841 caacgtgttt gtatcgcgcg ttgtttagca attgaacctg aagtcatttt aatggatgaa
     3901 ccgacatcag cattagatcc aatctcaaca ttaagagtag aagagttggt tcaagaacta
     3961 aaagaaaagt atacaattat tatggttaca cataatatgc aacaagcagc tcgtgtatca
     4021 gataaaactg catttttctt aaatggttat gtcaatgaat atgatgatac tgataaaatt
     4081 ttctctaacc catcaaacaa gaaaacagaa gattatattt caggaaggtt tggttgatat
     4141 ataatggcaa taattagaca gcgatatcag gagcaacttg atgatttaat aaaggaatta
     4201 cgtcggttag gtgcaaatgt ctatgtgagt attgaaaatg gtataaaatc attaagtatt
     4261 gacgatagag gctttgcacg acaaacagtt aaaaacgata aacatatcaa tcaattaaat
     4321 tatgatatta atgagcgagt tatcatgtta attacaaagc aacagcccat tgcgagtgat
     4381 ttgcgtatga tgatttcttc attaaaaatc gcctccgatt tagaaagaat aggagataat
     4441 gcatcgagta ttgccaatat tcgattgcgt acaaagatta cagatgatta tgtgttaacc
     4501 cgtttaaaga caatgggtaa attagctatg ttaatgttaa aggacttaga tcaagcattt
     4561 aaaaagaaag ataccgtatt aataagagaa ataattgagc gtgatgaaga tatcgatgac
     4621 ttatatagtc atattattaa cgcaacgtat cttattgata acgatccatt tgtcgctgca
     4681 caagctcatt tagcagcaag acatttagaa cgtattggtg atcatattat taacatcgct
     4741 gaaagtgttt atttttattt aacaggtaca cattacgaac aataacttaa agttattact
     4801 ataaaatccc ttacgataaa tatatatttc tatcattcat aaaccctcaa aaaaccaaga
     4861 ttctcacaat tagtaatgtg aaaatcttgg tttatattgt tctactataa attgtctcgc
     4921 atcttagtta tttgcttgct caatttcatc tgttaatttt tcaacttcat cgactaaatc
     4981 agaaatatat tgaattgtag atttaagtgg ctgttctgta gtaatgtcta cacctgcaat
     5041 gtttgcaagt tcgacaggtg atacactacc accttttttc aatgtttcta accaagcatc
     5101 aacagctggt tggccttcat ttttaatctt ttgagaaacg acagttccga ttgttaagcc
     5161 agcagaatac gtatacgaat ataatcccat atagtaatga ggttgacgca tccatgttaa
     5221 ttcagcaccc tcagtcatgt ctactgcatc tccaaaaaat tgtttataaa catttagcat
     5281 tatttcattt aatgtcggcg catttaaaga ttcaccttga tctactttgt gatacacttc
     5341 acgttgataa gcagcttcta ataaatgggt aaccatatta tgataatatg ttctagataa
     5401 aattgagcca ataacccaac gcttaaatct tggattatca cttgtgttaa ataaataatt
     5461 ggccatcaac atttcattca ttgtagaagg ggcttcaaca aagtacattg atgcttctga
     5521 ttcaagatat ggttgatgtt tttgagctaa tgtaaaatga cctgcatgac ctaattcatg
     5581 tgctaagaca aatgcttcag ccattttacc agtccaagaa ataaacacat atgaatgtgt
     5641 aaagtatgga cttgcacaaa atgcgcctgt atctttacct ttattttgtg caaaatcaat
     5701 ccatcgctga tcgtatgctt cacgtaacat gtttgtatag tcatcaccta aaacacttaa
     5761 cgcaccgaaa atataatttt ttgagtcttc aattgaaatc tctggttcat aatcagggtc
     5821 tacagaaatc ttcaagtctt caaaacgcat gttatctaat ccgtgaatac gttgtaaaag
     5881 tttagcatat ttctgcataa ctggtgccaa gtcacgcata atcatatcga tttgacggtc
     5941 aaacatatca cgcgttactt cttgactatg taataaataa tcgatgactg attcaaatcc
     6001 acgtaaatca gcttcaattt tttcttgttg tacttgcata ttatatgtag ccgcggtagt
     6061 atgctgatat tttcgaatcc catcgctaaa cgatttgaaa cttttacgtc taaactcagg
     6121 ctctttatta tcttcataat cattttcaaa cgtagcatag tcgacagggt acgttgtacc
     6181 atcatgttca aatgaatcga atgtaatatc tagcattttt gtcgtgccgt ataaatcgta
     6241 aggactgttt agcgtaggtg ataaagttgc taatacttgt tctaccgacg cagataattg
     6301 gaatggcttt tgttttatta actgtttaat atagtgttga tatggacatg attcttctaa
     6361 ttgttgaagt atttcttctg gtaattcaag tatttccgac tctacaaatg ataattggct
     6421 aacaatttta ccgtatgtag tagataattt agcgctcaat acttgtgcct cgatattact
     6481 agtatctaca cttaaacgta gttctgcata attacttaag cgatctaagg caattaaaat
     6541 attttctaat tcagctaaag cagtattaat ttgttcgatt gaatttaatg ttgttgcata
     6601 tgtatgatga aattgatttg cttgttgtac tagagcgtca atactttcat aatattgttg
     6661 atcatcttta aataagtcta ctaaatccca tgtttctgaa acaggaacat cttctcttaa
     6721 aggtaaacct tgagacatat aatcactcct ttggtataat acattcatta tacataattt
     6781 tgtctcctaa acgataatgt tacgatttcc atgtcgcaag gaacaatgat ttttctaata
     6841 gtagatagaa tattgcttga aactgactgc tattgttt
//
LOCUS       DBNENW010000132         6850 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNENW010000132 DBNENW010000000
VERSION     DBNENW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.133"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(20..580)
                     /gene="icaR"
                     /locus_tag="AC1LW8_002160"
     CDS             complement(20..580)
                     /gene="icaR"
                     /locus_tag="AC1LW8_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HIP4773965.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            744..1982
                     /gene="icaA"
                     /locus_tag="AC1LW8_002161"
     CDS             744..1982
                     /gene="icaA"
                     /locus_tag="AC1LW8_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase
                     IcaA"
                     /protein_id="HIP4773966.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESDTIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            1946..2251
                     /gene="icaD"
                     /locus_tag="AC1LW8_002162"
     CDS             1946..2251
                     /gene="icaD"
                     /locus_tag="AC1LW8_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein IcaD"
                     /protein_id="HIP4773967.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2248..3120
                     /gene="icaB"
                     /locus_tag="AC1LW8_002163"
     CDS             2248..3120
                     /gene="icaB"
                     /locus_tag="AC1LW8_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HIP4773968.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3107..4159
                     /gene="icaC"
                     /locus_tag="AC1LW8_002164"
     CDS             3107..4159
                     /gene="icaC"
                     /locus_tag="AC1LW8_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HIP4773969.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4605..6647)
                     /gene="lip1"
                     /locus_tag="AC1LW8_002165"
     CDS             complement(4605..6647)
                     /gene="lip1"
                     /locus_tag="AC1LW8_002165"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HIP4773970.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRQTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         2134 a         1122 c         1042 g         2552 t
ORIGIN      
        1 tacaaactag taacaaaaat tatttcttca aaaatatatt tagtagcgaa tacacttcat
       61 ctttgaattg acttttactt tcttccactg ctccaaattt ttgcgaaaag gatgctttca
      121 aataccaact ttcaagaaac agcaatatta aattctgaaa gtcttctttt gtcatcttta
      181 tctttgattc atcatagaat tttgctatct ctttacttaa tgattgattt aaatcttgta
      241 tttgtccgta aatatttcca gaaaattcct caggcgtatt agataattga acgtacattc
      301 taatatacct ttcttcgatg tcgaaaataa actcaaataa gaattgatat aaagcatcaa
      361 ttgaatagtt cgatttattt tgattcatca taataatatt attaaggtaa tcaaaacaac
      421 atttaacact ttgttcgtaa atactttttt tcgagtcaaa atggtaatat aaactcgctt
      481 tctttatatt tacactttta gctatatcat caagtgttgt accgtcatac cccttctctg
      541 aaaataaggt tattgcgtta tcaataatct tatccttcaa tttttataac cccctactga
      601 aaattaatca cactatgtta caggaaaatt aagttgcaat tacaaatatt tccgtttaat
      661 tataacaaca atctattgca aattaaaata ctatcaatta ccatatggct tacaacctaa
      721 ctaacgaaag gtaggtaaag aaattgcaat tttttaactt tttgcttttt tatcctgtat
      781 ttatgtctat ttactggatt gtcggttcaa tttatttcta ttttaccaga gaaattagat
      841 attcattgaa caagaagcct gacataaatg tggatgaatt agaaggcatt acatttttac
      901 ttgcctgtta taacgaaagt gacacgattg aagatacgtt gtctaatgtt cttgcactca
      961 aatacgagaa gaaagaaatt attatcatta atgatggaag ttcagataat acagcagaac
     1021 tcatctataa aatcaaagaa aataatgact ttattttcgt cgatttacaa gaaaacagag
     1081 gtaaagccaa cgcactcaat caaggcatta aacaggcttc atatgattat gtaatgtgct
     1141 tggatgcaga tactatcgtt gatcaagatg caccatatta tatgattgag aatttcaaac
     1201 atgatccaaa acttggtgca gttacaggta atcctagaat tcgaaataag agttctattt
     1261 taggtaaaat tcaaacgata gaatatgcaa gtttaattgg ctgtattaag cgaagtcaga
     1321 cacttgctgg cgcagtcaat actatttcgg gtgtcttcac tctatttaaa aaaagtgcag
     1381 ttgtcgacgt tggctactgg gatactgata tgattaccga agatattgca gtttcttgga
     1441 aattgcattt acgtggatat cgtattaagt atgaaccgct tgccatgtgt tggatgttgg
     1501 ttccagaaac attgggaggt ctttggaagc aacgcgtgag atgggctcaa gggggacacg
     1561 aagtattact acgagacttt tttagcacaa tgaaaacgaa aaggtttcct ttatatattt
     1621 tgatgtttga gcaaatcatc tcgattttat gggtatatat agtgcttcta tatttaggct
     1681 atttgttcat aacagcaaac ttcttagact atacatttat gacatatagt ttttcaatat
     1741 ttctactatc atcatttact atgactttta taaacgttat tcaatttaca gtcgcactct
     1801 ttattgatag tcgctacgag aaaaagaata tggctggact catatttgta agttggtatc
     1861 cgacagtata ctggattatt aacgcagcag tagttcttgt cgcatttcca aaagcattaa
     1921 aacgtaagaa aggtggttac gcaacatggt caagcccaga cagagggaat acccaacgct
     1981 aaaatcatcg ctaaatattg taagagaaac agcacttatc gctatatctt gtgtcttttg
     2041 gatatattgt ttagttgttc tactcgttta tattggtact atatttgaaa ttcatgacga
     2101 aagtatcaat acaatacgtg ttgctttaaa cattgaaaat actgaaattt tagatatatt
     2161 tgaaactatg ggcattttcg cgattatcat ttttgtattt tttacaatta gcatattgat
     2221 tcaaaaatgg cagagaggaa gagaatcgtg aagtatagaa aatttataat tttagtgttg
     2281 agtatcttga tcatattgcc tgtaagcaca ctggatggtc atcatattgc aaatgcagat
     2341 gacgattcac ctaaaaaact gaaatataaa gaaaatagtg ctctggcatt aaattatcac
     2401 cgtgtaagaa aagcgaattt tctgaataat tttatttact tcttttctag tagtaaagaa
     2461 attaaaaatt atagtgttag tcaatcacaa tttgaatctc aaataaaatg gctaaaatca
     2521 catgatgcta aatttttaac cttgaaagaa tttttatatt acaagaaaaa aggtaagttt
     2581 ccaaaacgaa gtgtatggat taactttgat gatatggatg aaactattta tgaaaatgct
     2641 tatccaatct taaaaaaata taaaataccg gcaactgggt ttattatcac aggtcatgtt
     2701 ggggaagaaa actttcacaa cctcgatatg attagtaaaa aagaactaaa agaaatgtat
     2761 aaaactgggt tatgggaatt tgaaacacat acccacgatt tgcataactt atctaaaaat
     2821 aataagtcaa aattaatgaa agcttctgaa gctacaatca taaaagattt aaacaaaagt
     2881 gaaaaatatc taactaaaaa ctttaaaaag tcgcagaaaa ctatagccta tccttatggc
     2941 ttgatgaatg acgataaatt accggtaatc aaaaaagctg ggttaaaata cggtttttca
     3001 ttagaggaaa aagcagtcac tccgaactcc aatgattatt acatccctag aatattaatt
     3061 agtgatgatg cttttgagca tttaattaag agatgggacg gattccatga aaaagattag
     3121 acttgaactc gtatatttac gtgctattat atgtgcaatt attattatca cacatttact
     3181 tacacaaatt actttaaaac atgaaaatat ggagggtgga tccttagtgt tacaatttta
     3241 cattcgtaat attgtgattt ttggtacacc ttgctttatt atcttgtcac agttactgac
     3301 aaccttgaat taccaaaaag tcacctatag atacttaact acacgcgtaa aatatatact
     3361 tattccttac atattaatgg gattgtttta cagttatagt gaatcattat taacagattc
     3421 aagtttcaat aaacaattca ttgaaaatgt cctattaggt caatggtatg gctattttat
     3481 cgttgttatc atgcaattct ttattttgag ttatatcatt tttaaaatta actataacct
     3541 attcaacagt aaaatattat tattgttatc ttttatttta cagcaatcat ttttatatta
     3601 ctttacgaac aacacagcgt ttcacgatac cgtgctacac tattatccat taagtgaaaa
     3661 tactataata ttcggatgga ttttttattt cttcttaggt gcatatatgg gttataacta
     3721 cgaacgtgta ttaaatttct tagaacgtta tttagttatt atgattgtat tagctgtagc
     3781 tacttatttt gtgtttattg cgttagcaaa tggagactat tggaacgtta ccagcttttc
     3841 atattcatta acaccatata atagtattat gtttattgtt atcttgggta tttgcacgca
     3901 ttttaaaaca atgttattta atacgattca aatgattagt gctttctcat tctttattta
     3961 tttattacat ccaatcattc tagactcatt gtttgcatat acaaatatat ttgaggataa
     4021 tacaatggtc tttctagcga tatcactact attcatttta ggattatgta taggtgtcgg
     4081 catgatattg cgtgaattct atatctttag gtttattatt ggaaaacaac catataaatt
     4141 gaacattaat gcttattaat tattaagcta tgttaaaaac acgcggtggg cgaaatcagt
     4201 ttgaattgac tgacttcgtt ttaccgcgtg tttaatattg ttatacatat attctaattg
     4261 cacatttaaa cttcgtaaat gccaatggga gtgggacaga aatgatattt tcgcaaaatt
     4321 atttcgtcgt cccaccccaa cttgcacatt attgtaacct gactttccgc cagcttctat
     4381 gttggggccc cgccaacttg cacattattg taagctgact ttccgccagc ttctttgttg
     4441 gggccccgcc aacttgcatt gtttgtagaa tttcttttcg aaattcttta tgttggggcc
     4501 tcgccaatgt tttacttgaa taattctttt agaattctaa ataatgatcc gattaattga
     4561 aagaagtctg cagtcattat taattcctcc ctttacttta taaattatgc ttgcttagta
     4621 tcagtcagct tttcagtttt cactaaatcg tctgctaaat gatgccaaaa atcttgtaat
     4681 tcttctcttg tgcgcactgt atcagaactg tcttgtccta caaagtcaac atgatcccaa
     4741 tcatgttttg taggcgtcac ttgccaaatg cctttttgaa ttttatctgt cgcttttgta
     4801 taagcttgat taaatggatg ttgagaagaa ataacggata ctaaaccatc gttttctcgc
     4861 cattcttttt cagtagcttt accgattaag ttaccagtaa tcacaaatgg gaaaaacata
     4921 tttaagtctg ctttttgtct atcgctattt aatgctttgt gcgttgcttc accagtgtat
     4981 gttttataca caatgttagg gttcaacgac gtttgacgat ttaaatctgt tgcaccctca
     5041 cgcgtcagat cgtaaaatcc attatctttt gatttccata aattagattg tttaacgcgt
     5101 ttgacataat caatatatga ttcatttggc ttctgtttta gaccccattg agccaacccg
     5161 aagtctactc ttgaattttt attaccaaac attttaccga tatcaaatac gatttgtctc
     5221 actaaagctt cattaccagc taaatctgat gcgtgtgtac cattatgtgg tgttcctaaa
     5281 gtagtaattg atgaaatcat attgtcatga ttacctttga atagtggaga aatttcgcca
     5341 ccatgtttct tttgatactc tatttcttca cgattaccat tacgcagtaa ttcttctagt
     5401 tgacgtatcg tttgaccgcc catactatgt ccaactaggt gtaccttctg tcctggtttc
     5461 cagtctttgt aaattccttc gtatgttttt ccataacgtt catgtccata ttttgctgca
     5521 tgtgctgcac cataatctac acgaccgcct ttgatataat aataaagttc aactgcgcgg
     5581 tcatagttac ttccaaaagc acttatactt gcttcataag ctttgtaacc attttcttct
     5641 aaatcttggc gaatgttcat tttattaccg ccccaataat gagctaacac tgaaggatta
     5701 atatcatctg taaacccatt gaaaccatgc actaaaacga taggatcctg atttttatac
     5761 tgcccttgtt tggcaacttt attcgtttga tcatctttcg cttttgcagt aagttccttt
     5821 gtccctttgt ttggtaacgt tgctactgca ctacttttta ataaatttaa tccatttttc
     5881 ttgtcattgt cctttgttgg ttctactggt gatttttgat tgttttttaa atctgaaact
     5941 gatagtgttt tttcgtcttt ttgaggtttc acttcttgtt cattgttaaa attgtagtta
     6001 gtaattttat cattcgcttg attattttgt tgtggttgta ctttctctat ttctgtctca
     6061 ttaacaattt ctcgttgact tgtttgatct aacttattta atgatggtgc ttcctttatt
     6121 cgactttgtg aaggtagatt gttattagcg tttgaatctt gactattatc tgcctctttt
     6181 gactgttgct gtgacgccac tttactttgt ttaccatcat taatagcctg ttctaaccct
     6241 tgatttacat cgctaggatt agcattgtta ttttttgttg tatcacgttg aatattttta
     6301 ttattaagcg atgatttttt atcatccttt agttgatctt tattcaaatc atctgtttta
     6361 cgatgatttt cttctgatat tgttttatca ttatgcaagt tcttagttaa attttcagta
     6421 tgttgctgtt cgtcaacacc gttttcacgt tgataattag cattttccct agtttgttga
     6481 tccccaatag attgtgctgt aactgtatcc tcctgtgaat ttcccatatt cacttgcttc
     6541 tcagctgctt gtgcttgtcc accacttaaa aatagtaatg tagctattaa aatggaagat
     6601 gcacctacac taaatttacg aatactatac ttattttgac ttttcatcat tgtcagcacc
     6661 tctatttttt tattttagat aaacaaattg ataattattc gaggaataaa ctcaaataat
     6721 ttacttaacc aagcactcat aatgaccatc ctttctattg tttaatttga cgagaaagcg
     6781 tttacatttg tatttaaaaa atatattctc caaagagaat ttaaagtgta tacgctaatc
     6841 atttcaaatt
//
LOCUS       DBNENW010000133         6740 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNENW010000133 DBNENW010000000
VERSION     DBNENW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.134"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..76)
                     /locus_tag="AC1LW8_002166"
     CDS             complement(<1..76)
                     /locus_tag="AC1LW8_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773971.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNF"
     gene            114..575
                     /gene="tsaE"
                     /locus_tag="AC1LW8_002167"
     CDS             114..575
                     /gene="tsaE"
                     /locus_tag="AC1LW8_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIP4773972.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            556..1218
                     /gene="tsaB"
                     /locus_tag="AC1LW8_002168"
     CDS             556..1218
                     /gene="tsaB"
                     /locus_tag="AC1LW8_002168"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIP4773973.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKILAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1191..1655
                     /gene="rimI"
                     /locus_tag="AC1LW8_002169"
     CDS             1191..1655
                     /gene="rimI"
                     /locus_tag="AC1LW8_002169"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIP4773974.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1648..2673
                     /gene="tsaD"
                     /locus_tag="AC1LW8_002170"
     CDS             1648..2673
                     /gene="tsaD"
                     /locus_tag="AC1LW8_002170"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIP4773975.1"
                     /translation="MTKDILILAVETSCDETSVSFIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2775..4385)
                     /locus_tag="AC1LW8_002171"
     CDS             complement(2775..4385)
                     /locus_tag="AC1LW8_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HIP4773976.1"
                     /translation="MSTNQTFLIFVIAIIPLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4474..4602
                     /locus_tag="AC1LW8_002172"
     CDS             4474..4602
                     /locus_tag="AC1LW8_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773977.1"
                     /translation="MIKTMSIENMCTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4748..6676)
                     /gene="abc-f"
                     /locus_tag="AC1LW8_002173"
     CDS             complement(4748..6676)
                     /gene="abc-f"
                     /locus_tag="AC1LW8_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIP4773978.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIITGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT         2214 a          988 c         1161 g         2377 t
ORIGIN      
        1 agaaatttaa gttgaaattt gttacaatat aaaggttaaa tataaagtta gatacgattg
       61 aacaatggat aatcattttt atgaatgaaa agcataatat aggagaatca acattgataa
      121 agataaataa tttagatgaa atgaatcaat ttgctatatt tttagttgag caattgaaaa
      181 gtggtgattt gattttactt aacggagatt taggagcagg taaaacaacg ttaacgcaat
      241 ttataggaaa agctcttggt gtaagacgta cgattaattc cccgacattt aacatcatta
      301 aatcatatag gggtaaaaat ttaaaattgc atcatatgga ttgttatcgc ttagaagatt
      361 ctgatgaaga tttaggattt gatgaatttt tcgaagatca ggcaattact gttattgaat
      421 ggagtcaatt tataaaagat ttacttccag cgacgcattt atctattaat atttcaacaa
      481 tatctgaaaa tacaagacaa attgagttgt tcgcgcaagg agaacattat gaacaaatta
      541 aggaggcaat tattcatgaa ttcgctgctc attgatacat cgaatcaacc attatcagta
      601 gcattgatgc aaaacgatga agttttagct gaaataacta cggattctaa acaaaatcat
      661 tcagtgcaat taatgcctgc gattagtcag ctatttgagc agagcaaaat agctaaacag
      721 caattagatg cgattattgt tgctgaaggt cctggttcat atactggctt acgtatcggt
      781 gttactgttg ctaaaatatt agcgtatgcg ttagatgtta aattatatgg tgtttcatca
      841 ttaaaggcat tggcagcaac aatcgaccat actgataaat tattggttcc tgtgtttgat
      901 gcaagacgac aagcagttta tacaggcata ttccagtggc aaaatggtca attagtgact
      961 attttagaag atcaatatat gtcgatagag gcattgcgag cttttttaga agatttaaac
     1021 caaccattta tttatattgg gaaagatact ataaaattac aagatgaatt acaaggtgaa
     1081 gtgattgcgc agctaccaaa tgcatcagtc atgtatcaat tgatagataa accttcagac
     1141 gtacatacat ttactcctaa ataccataaa ttagctgagg cggaacgaaa ttggatcaac
     1201 agtcaaaaga acaattaaac attagagaga tgactaaaga agatgtgcca caagtctttg
     1261 atattgagcg tcggagtttc aatgacagtt cttggacaat tgacgcattt tatcatgaaa
     1321 tagagcaaaa taactttgcg aaatattttg tcttagaatt tgaacaacaa attattggtt
     1381 atttaggttt atggattgtt atcgatcaag ctcaaattac aacagttgca atcgatgata
     1441 attatagagg ctatggttta ggtcaaatgc ttttaaaata cggaataaac tatgcaagtc
     1501 acacttgtga tgtgatgagt ttagaagtaa gagttaataa taaagtggca caacatgtat
     1561 atgaaaattt aggatttcaa tatggtggta aacgtaaaaa ttattatggt gaaggtgagg
     1621 acgcaatggt tatgtgggtg aatttaaatg actaaagata tattaatact agctgttgaa
     1681 acaagttgtg atgaaacaag cgttagtttt ataaaaaatg gcagagatat tttatcaaat
     1741 acagttttaa gtcagattga aagtcataaa cgatttggcg gtgtcgttcc cgaagtggca
     1801 agtagacatc acgttgaagg tataacaaca acaataaacg aggctctagt ggatgccgat
     1861 gtatcaatag aagatattga tgccatagcg gttacagaag gccctggact aattggtgcg
     1921 ttactaatag gtgttaatgc agccaaagca ttggcatttg cttacgataa gccacttatt
     1981 cctgttcatc atattgcagg acatatatat gctaatcaca tagaagagcc attaacattc
     2041 ccgctaattg cacttattgt ttcaggtgga catactgaat tagtttatat gaaggatcat
     2101 ttatcatttg aagtcattgg tgaaacacga gatgacgcag taggtgaggc ttatgataaa
     2161 gtggcacgaa caattggttt aaattatcca ggtggtccac aagttgatcg gttggctgct
     2221 gaaggtgaag atacttattc attccctcgt gtttggttgg ataaagatag ttatgatttt
     2281 agttttagtg ggttgaaaag tgccgtaatc aatcaacttc acaatcaacg acaaaaaaat
     2341 attccaatca ttgaagctaa cgtagcaacg agctttcaaa atagtgttgt agaggtgctt
     2401 acgtttaaag ctattcaagc ttgtaaagaa tatggtgttc agcgattaat tgttgctggt
     2461 ggcgtggcga gtaataaagg attacgtcaa tctttagcgg atcaatgcaa agtcaatgac
     2521 attcaattaa ctatcccaag tcctaaatta tgcacagata atgctgcaat gataggcgtt
     2581 gccggccact atttgtatca gcaaggtcga tttgctgatt tagcattaaa tgggcacagc
     2641 aatatagatt tagaagagta ttctgcagaa taaaataaac gtcagttatt aacatgatta
     2701 cgatgaacat tattgaaact gttcatagtc ttctgtcatg tgatagctga cgtttttata
     2761 tttaaagtta aatgctaaat tttcgggaca ttatcttttg ctcgttcata tatttttgct
     2821 ggaaatgaag tgatttttaa taattcgatg gcattacgtg tatttgcttt gccaggctta
     2881 attttgtaat caaaatgtat gttattattt tctattacct cattgaaatg ataattttca
     2941 taacgttgtt ttaagagttc agccaactca atatcatgtg ttgctgcaat aactctaaag
     3001 ttagattttt catgtaaaaa tgatagtact gattctgaag cggcaattcg ttcagttgtg
     3061 ttggtacctt taaaaatttc atctataaag cagtatattt tttgattatc tggaatttca
     3121 acaatacgct taatagattt taattcagcc ataaaataac tatcgcctga taacacatca
     3181 tcggcatttg ccattgacgt gaacacaatt ccaggttgat atacaaattt gcttgcggta
     3241 actgtttgta tagccgatgc aagaataatg tttattgcta tggatttcat aaatgttgat
     3301 ttacccgaag cattagaccc agttaacagt atattttgtg atagtgagaa atcattggct
     3361 actgcatctg ctatgagtgg atgtgttaac tcagagaata ctatgccatc atttgaatca
     3421 tcaatttgtg gttcagtata acattctagc gttctacgat acattgctaa tgcataatga
     3481 ttatctaatt cagctatgta atcgaaacaa tttttaagct cattcatatg tgttgtatag
     3541 cttttttgaa ttgaatgaaa taaaacataa tcaagcatga aaataagttt aactagttta
     3601 attaccatag ctccgatatc ttcatcatta acttcagcta aaacactcgt taaatgacgt
     3661 gctgttctaa attgcttaaa attgacttca ggttgtggag catgtttaat ttttgagata
     3721 gtatagcctt gttttaaaac attggatgca taaaagatag attttaagtc atcctcatat
     3781 gtacgcttta aagttgctga taaaatgata ttaaataggc aactcataag aaataataaa
     3841 ataccaactt gtgaattaat aaatataact gcaaatgaga taactggtaa aaacgggcat
     3901 agaaacaata tattttgacg ttttacaggt gtgatttggt ctggaaatgt tggataaaca
     3961 gttttaccaa tcaaagctaa atgatatgtt acgttttttc taaattcagc attatcatta
     4021 aataaactaa gtaatgattt gttcgtaatt gaaagcattc cacgtaaaca ggcatatagt
     4081 ttcatttcac caatagctgt taaattataa ttcatcttat gaaagattgc attcatattt
     4141 aagtcggacc aagttttatc atcaactaat gaatgtgatt caaatttttc cttgtagagc
     4201 ttatactcat agtcgaaatg tgagttcggt ctgataaaag cttctaatgg agaaatcgtt
     4261 tgccataatg tttccataga ttttaataga cgttgacgac tcatgtatcg tccaacaatt
     4321 cctattacag atgtaagtgg aataattgct ataacaaata ttaaaaatgt ttgatttgtg
     4381 ctcataagta tctccttatt tatttttaat aataataaaa atatacttca atattattat
     4441 tatttcataa agaaaatgtc ttaacaatga attttgataa aaacaatgtc aatagagaac
     4501 atgtgtactt atcaacaaag agtgaataac ttgtggataa aaggtgttga taacgtcatt
     4561 tattgtggat atcaggtggg aaaacagaaa ataaatgatt gattagtgtt aatttacttg
     4621 tggataacta ggataactct gtggatagct ataatatcag cgtttttatt gtgaataaaa
     4681 aaatttaaaa tttgtaaaaa aaataactct aactatagcg attttacagt tagagttatg
     4741 tatattttta tagtttttgt tgtaattctt cccatttaga catagcgtgt tctaattttt
     4801 gttcgctatc ttgtttttgg atagctaatt cgtttgcttt ttgtggattg ttataaactt
     4861 ctggttgggt taattgctcg tctatttgta agatagtagt ctctaaagtt tctatttcat
     4921 tttcacactc ggatatttgt cgttcaagtt tgcgttgttc acgtctttgt tctttttggt
     4981 tatgatacga cgattgttcc atagactttt catgtgcttg tacgttaact gactgttctt
     5041 cttggtgcgc tttcaacgct tccgcttcct caactttttc aatgtaatat tgataatctc
     5101 ctaaatacat ctttccgcca tcaattgtta aatcaaatac cttgtttgct aattggttaa
     5161 taaagtaacg atcatgggaa acaaatagta ttgttccttc aaaatgttgg agtgcttgct
     5221 ctaacatttc ttttgaatct atatcgaggt ggttggtagg ttcatctaaa ataagtacat
     5281 tgtcacgttg caacataagt agtgctagtt gtaaacgtgc tttttcacca ccagataaat
     5341 cattaattat ctttttaaca tcgtcttgta caaataagaa acgaccaaga actgcacgaa
     5401 tatctttttc attcattaac ggatattgat cccacacata atctaaaatc gttttactag
     5461 atttaaattc tgcttgtttt tgatcataat aaccaatttg taaatttgcg ccgaaagtaa
     5521 tatcgccatt aagcgctttt tgttgattag caatagtttt aattaaggtc gattttccaa
     5581 taccatttgg cccaatgatt gctatatgat cgcctttaga gacctctata ctgataggtt
     5641 tggtaattgc agtttgataa ccgatttcta aattttttac atgcatgacg tcattacctg
     5701 tattccggtc aaagccaaat tgaatatttg cacttttggc atctaacatt ggtttatcaa
     5761 tgcgttccat tttttctaaa atcttacgtc tactttttgc cattccactt gttgaagcac
     5821 gggtaatatt tttctcaaca aaagtttcta atcgttttat ttctgcttgt tgactttcat
     5881 attcttgcat tcgtttttga taatataaat cccgttgctg tataaattcc tcgtaattac
     5941 caacatagcg tttgacatca cctaaagcca catcataaat ttgagtaact attttatcta
     6001 aaaagtaacg atcatggctg atgatgacga ttgcaccttt aaaataacgt agataatctt
     6061 caagccactt tgtcgtttct aaatctaagt ggttagtagg ttcatctaaa agtaataaat
     6121 caggttcgtt taatagcatt tgagctaaag ataaacgtgt tttttggcca ccgctaaaat
     6181 cattgatagg tttattgaaa tcttcttcac taaaatttaa cccgtgaagt actgttttaa
     6241 ttttactttc atattgatac ccttctaatt gttcaaattg attcgataaa gattcataac
     6301 gggacatatg tgttttatat gtatcactat cgtaatcatt tgcatgtttt gataaccaat
     6361 ctgtttcttc tttgattaag ctttccattc gtttaatatg ttcaaatggt tttgacattt
     6421 cttcaaaaac tgttgcatta gaatttaaag tcatttgttg agttaaataa cctagtttta
     6481 agtttttaat ttttgacaca tttccagaat catagttttc tacgccagtt ataattttca
     6541 tcaatgttga tttaccggcg ccatttcttc ctacgatacc aattcgttca ccagttttta
     6601 cttcaaaatc aacatcagta aatatatctt caccatcgaa cgattttgat atatgattga
     6661 gttgtaaaag tatcattcga tcttcacctt tcgtctttct caaattttat taacttatga
     6721 aaaaagtcgt aattattaca
//
LOCUS       DBNENW010000134         6687 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNENW010000134 DBNENW010000000
VERSION     DBNENW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.136"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(214..1842)
                     /gene="ccrB"
                     /locus_tag="AC1LW8_002174"
     CDS             complement(214..1842)
                     /gene="ccrB"
                     /locus_tag="AC1LW8_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cassette chromosome recombinase CcrB"
                     /protein_id="HIP4773979.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(1864..3213)
                     /gene="ccrA"
                     /locus_tag="AC1LW8_002175"
     CDS             complement(1864..3213)
                     /gene="ccrA"
                     /locus_tag="AC1LW8_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cassette chromosome recombinase CcrA"
                     /protein_id="HIP4773980.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(3447..5234)
                     /gene="cch1"
                     /locus_tag="AC1LW8_002176"
     CDS             complement(3447..5234)
                     /gene="cch1"
                     /locus_tag="AC1LW8_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cassette chromosome replicative helicase"
                     /protein_id="HIP4773981.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(5234..5524)
                     /gene="ssb"
                     /locus_tag="AC1LW8_002177"
     CDS             complement(5234..5524)
                     /gene="ssb"
                     /locus_tag="AC1LW8_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cassette chromosome ssDNA-binding protein"
                     /protein_id="HIP4773982.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            5663..>6687
                     /locus_tag="AC1LW8_002178"
     CDS             5663..>6687
                     /locus_tag="AC1LW8_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HIP4773983.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFF"
BASE COUNT         2069 a         1037 c         1147 g         2434 t
ORIGIN      
        1 gcaaatccca atcatttccc gataaaaaat cattgtggga tgttctttag caatttcaat
       61 ataagcattg tatagttatg aaaaaattac gacaataact gtttcattag ataagtgtta
      121 ttgaaattga taaagagcaa ttcttgaaaa tagttagata aaataagcga aagaatatag
      181 tgaaaattat tgttataaca atgattctat tagctaaata gtaagatata gtgtttgggg
      241 caaaaataaa gacgaagtgc tgagatgcac ttcgtcgagt tgtttattat tgaaaagttg
      301 tttaatgatt tcgttattaa gtttgagtgt gacatagaat tgttttttat gattaccatc
      361 ttttttaata tcaatgcgat caatcactga tagatacaat gctttgagtc gagatttttc
      421 tatgtgctta atatcatgaa agatgtgttg taatagttta ctgatttctt tggcatcaaa
      481 taaagtctta tcttcatttt gttgattttt gagttggttg atttgattcg taatgtcatt
      541 gagttgcttt tcatattttt gaatacttgg tctgattact gatgttaagt ccggattatc
      601 ctcgatggtt ttaatcaagt tatttatttt gatttgtact tcatcatatt gttgttgctt
      661 ataagcaata tcgtgatgaa gtgcagcgcc atcaacttga ttttcttgat tgacgtgtgt
      721 tactacgcgt tgaatgactt tatcactttt gactatttca agtatttgct tcatcacata
      781 atcttcaatc acatcagctc ttacactgtt tgccgaacat actttggaac ccttgttccg
      841 aaaattacta catgaatagt aacgaatacg tttcttagtc ccgtctttaa gagtattcgt
      901 ggtattgctt gctgccatag gtgcgccaca ttggggacag tgaataatgc ctgtaagcag
      961 attcgttcct ttgccatgga cttggggttt ttgactgact tgtttcttac gcatttgtac
     1021 tttatcccat aaatcttgat taataatggg ggaatgctta ccttcagcta tcactggttt
     1081 atcattcagc cctttacgac gtttttcact ccaatctttg tatttcgcaa attgaatttt
     1141 gccgatatag aaagggttag ctaagatgta tgtgattgaa ctaatactga aaggtttccc
     1201 ctttttagtg acatatcctt tgtgattcaa tgcattggca attttacgat agccatgtcc
     1261 tttggcatag cactcgaata tatattttac aatattagct tcatgttggt taatcattag
     1321 ctcgtgttta ctatctggta ttttgtcata acctagtggt aaattgcctt gataatagcc
     1381 ttcttgggca cgtctcgttt gacccataaa tacgttctcg acaatgttat tacgttcgaa
     1441 ttctgagaaa ctcgcaagta tctgtaacat gagtttacca gaagaagtat tgacttccat
     1501 acgctctgac aaactgaaaa attcgacatt ttgtttgtgt aaatcttcga caattttgag
     1561 aagatcagat gtattacgag ctaatcggtt tgttttgtat accataacac agtcgatatt
     1621 gccttctttt gcatctttca acatacgttg gagctcaggt ctgttcatag atttacctga
     1681 aataccacgg tcagcgtata tatctttaac ttcaaaatga tggaagtcac agtattcttt
     1741 gatttgattg atttgtccgt cgatactata accttctgtg ctttgcattt ctgttgatac
     1801 acgtacatag ataccgacac gttttgtttt aagttgttgc attatgttac atcctttctt
     1861 catttatgca atcgatgatt gcatggtttg attgacaata ttgagtggtt catttttgaa
     1921 atagattcct ataagatttt tatctttcgt aatgtgaatg gtttcaatat aggggtacaa
     1981 tatgtttaac gtgaaacgtt tttgaataat attttgaatg atgtgttgta tttgatgccc
     2041 attgatagat gtagtgcgtt gcggttgttg acgtaatgat tgtgtttgct ctctgaacgt
     2101 ttctgcatca atgatgcctt gtgccaattt ttctatcagt tgttcttgag tcaatgtgtg
     2161 atgtttttct atgtttcttt gtcttttgat gcgtttgtca atcgcacttt taatttttgt
     2221 gtagatgcgt tgattttgat aaaagtctcg gcacacttct aatactttat cttcaagtgt
     2281 ttgtgcattg atgcctttaa aatcacagac aaagcgtgaa gcattcatgt ttttaggaca
     2341 gacgtagtaa cgtaatgtat gattcttttt tctaatggtc atatttgtaa gtgttgcatt
     2401 acaacatggg catttgattt tttgtttgag ttgattatcc gaaggtgtct gtttggtttg
     2461 tttttgcaat cgaagtctct gcgcttgctc atatatactt gtggaaacaa tagaaggaaa
     2521 catattgtcg aattggccat attgattgtt gacacgacca caataattag gattgatgat
     2581 aatgttacga acttgatagg gttgtcgatt gatatacgtg ttatcttctt ctaataactg
     2641 tgcaattttc ttataaccat gacctttaat gtaataattg aatacagcct ttaccgttgg
     2701 tgactcattt tgattgatga tgaatgttcc gttgtgatat tcgtaaccaa aaggtgcatg
     2761 tgttgtaatc aatcgacctt gctttgcttt ttcttgaagc ccatttctga cttgttctcc
     2821 aatgttatcc gattcaagtt cggctaaact gatgaagata ttaagcttga gtcggtcgaa
     2881 agcttgatcc atatcaaagt aaccatcatg tacgcttaag atatgaacat ggtacgtttg
     2941 acataatttg atgagtttta atgcattttt cagattacga tgcaacctat taagacgata
     3001 acagcataat atgtcacact gtccttgttg aattaattgt gttatttgtc gatacccact
     3061 acgattatct ttgcgacctg attgtttgtc gctataaaag ttgatatgtt gaatatgatg
     3121 tttttcggct attgcttcga tagcttgttt ctgtgctgca agagattgtt gtttcatcgt
     3181 actttgacgt aaatagccta tgacttgttt catatcggct cctcctttca cagtgataat
     3241 atatatttat ggatgaattg atatataagc ccaacatcaa tgagatgttg ggcgtccata
     3301 ttagtcattt gtttgattga tttcttcaat taccaaatcg gctaatatct cgataagttc
     3361 atccatgttt ttcactccgt tatttgttct atcttcaata cgtcgattat tcagtttgat
     3421 gcttcacagt tgtatgataa agacaattag aaatcttcgt gaactcctga agggcctatc
     3481 ccttcattag cggatttaaa aagttctttc gcagctttgt tatcatttga cggtgtccaa
     3541 ttttgaagta acgacttatc tttagttaat ccgaggatag atgcaaactc tacatctaat
     3601 tttagatggt aaaatacaag tgattgtttt ttaccgctat tatctttgac acttctttta
     3661 gttgtttggc gtccacggtc agctaatatg aaacctttat ctcttaaggc gttgacaaca
     3721 ttattaacat cttgaaattg atgattgttt agcatctgtt taaaaacgtt cgcaattatt
     3781 tttacttcga tatggtcatc ttttaatgag attaatccat agttctcaaa catatttttc
     3841 aaagcacctt catctgaaaa cttacctctg ttttgtgcca caaattgaat gatgacatca
     3901 atagctttat cagctaatga gcgttcagag actgtatgag tatgataatc aataaagtag
     3961 tctcttatat tagcgatatc aatatctgta gataaaacac gacctaatat tttcgcagat
     4021 gttgtaatga ctgcataacg cttaaacata cgaatacctg tattgtttgt ttcatctttc
     4081 aatttagctt caaaccaatc tacttccttg taaaaccatt gaataacttc atcttcacga
     4141 tttataagat atttagctac taacggtaaa acatgaccat agtttagtgc tacagctttt
     4201 ttaatattgt cagcattggt cgcatttgta gtgaattgtt cattaatctc gatggttctt
     4261 acacgtaatc catcgttttg agctgaatca ttaaaaatac tgtattctga cgttgaaatg
     4321 acagaagtac cccaattctt aggcgtttta acttctccat gaacgtttga acgttgacga
     4381 ccttgacctt cagcgatgga atataacaaa cccgtggtat ctctaagtgt cgtagatgaa
     4441 agctcatcaa atactatagg aatgccataa ttgttactca aataaccttc aagtgcgttt
     4501 cgtgtggcat tccaacttct aaaaagagtt tcattacctt tggtagggtt accagcgact
     4561 gatacagcta aagcagctgc ggttgactta ccggttgagg attgacctgt aaaactaaag
     4621 agaattcctg caaattcgat ttcatgttta tgcttcagaa aacttgtcac taaggcagaa
     4681 atcccaaata tgactgccaa ttctaaaaga agatgacctt taacctcgtc aatatacatg
     4741 ttaaaccaat tatcaaatgt acctttagga gttaagtcat aagcactatc tacaataggg
     4801 tatgaaggtg aggactgatt aaattgaata gatttgagaa ctttatctag cgatatcaag
     4861 tagccaaaag gtgtctctag tatacctaca ccctcatata attcagaaag tggtaatcta
     4921 tctcgcatca attgcaaggc ataactcaat gatctaatat acttctcgtt aatactgtag
     4981 ccatatttaa ttaaagaagg taagtttcgt gttgttaaga tatcagactc aaaaatgtct
     5041 tttttaccat tgttgttagt aataatcaac ttttcaacac cagtaattgg atcagaaaat
     5101 cttgcattaa caataatagg gctagccatt ctaaccactt tttcactgtc accatctttt
     5161 ttaggtggta tcgtttcttt ccaaccgaag gaatctaaag caaatgggcc aatttcaaac
     5221 aatgtgtaac tcattagcaa tcacctcctt tcgaaggcgt gctattattg catgggttta
     5281 tccctatttt ctgatataat aaactgccat tttttgttcc tacaatttta aatggtaatc
     5341 ctggcgtgta cttaatccag taagcaaaac gacgtcctac ttcacgttga tcattcgtgc
     5401 tacaaaccac attatatttc tttcttaatt caccataagt gaacgtactt ccaactggtt
     5461 gttgaaaaga tttagcaatt ataaaattat aaagcttttc ttgctgctta gatttcttat
     5521 tcattttaaa attcctccaa taatttttaa ggagtcaatc aatatcatat aggttaaact
     5581 atcttcaatt gattgctata ataagcataa atgaagcatt atgattagac gcggaacaaa
     5641 aaatggggag ggggggtata tattgatacc tacgctagaa gaaattattg ataagtatgg
     5701 aaacttagtt gattatttga aatttgatgt aacagttgag gtttatgaag atttgttgtt
     5761 attaagaagt ttaagcgatt taaatgaaca tcaaaagatg gatagagttt catttataaa
     5821 aaaacattta aactcaaatt tcgattattt attagatgat actgagttta gaagtatcaa
     5881 taagatttac agtaatttaa atatcatgac gcatatcaat agtcaaaata ataattttaa
     5941 acgtaatcca tttataaatg aagaacaatt gaaatcacta ctcgcgataa agaaagtaga
     6001 tgcacatatg agttatgatt caaatattac ttcaaaagca ttggcagaaa taaacaaaac
     6061 gcaaaaaaga ttagttacaa agatagacac actctataat cagtcaaaaa aagaaaatga
     6121 atatgtacgg ttaggagaaa aaatatcgta tacaaaattg gagaatcatt ggaaatattt
     6181 atataatgag attcagtttt ataatcttaa taatcaattg attagttatg ttgcattaga
     6241 acaggaatat gcttgggctt ttttaaatga actattttat ttgatagaat tgtatttgaa
     6301 ggcatttaaa ggacaaaaga aaacagatta tgagtttggg aaaaaattaa acgaatttat
     6361 tcaatcatat gtcattattt tacttataga tatacgatta cctgttgtta gattatatat
     6421 tattagagac attgtagata catgtgaagg agaaaaagat aattacaaaa gaattacact
     6481 gatagaagaa tctattaaaa agtatagata cgtaaaagat cagattaaaa gatttaaaaa
     6541 tggattagaa gaatcgttat taaatgcaac tttatctatt gaacaaaata tgttgaaagt
     6601 taaaattgat tattataaag cctactgctt tcctagagaa aaaacaaagc ataacaatat
     6661 tgagtacaat gtatcgttgt tttttaa
//
LOCUS       DBNENW010000135         6531 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.137, whole genome shotgun sequence.
ACCESSION   DBNENW010000135 DBNENW010000000
VERSION     DBNENW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.137"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..3387
                     /locus_tag="AC1LW8_002179"
     CDS             <1..3387
                     /locus_tag="AC1LW8_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIP4773984.1"
                     /translation="INRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGL
                     GNQEAKAAENTSTENAKQDDATTSDNKEVVSEAENNSTTENDSTNPIKKETNTDSQPE
                     AKEESTKSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNT
                     NNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEE
                     LKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVND
                     LIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDL
                     TDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKTV
                     PNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVNP
                     LKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRIYDF
                     SQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRT
                     TDKYGYYNYAGYSNFIVTSNDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKE
                     KPMANVLVTLTYPDGTTKSVRTDAKGHYEFGGLKDGETYTVKFETPTGYLPTKVNGTT
                     DGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKD
                     VKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKDS
                     NGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDGNKDGKQDSTEKGIKDVTVTLQ
                     NEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVD
                     VTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKA
                     KALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
     gene            3506..4978
                     /gene="sdgA"
                     /locus_tag="AC1LW8_002180"
     CDS             3506..4978
                     /gene="sdgA"
                     /locus_tag="AC1LW8_002180"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-aspartate repeat adhesin
                     O-glycosyltransferase SdgA"
                     /protein_id="HIP4773985.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHHLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLADTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QVEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5102..>6531)
                     /gene="sdgB"
                     /locus_tag="AC1LW8_002181"
     CDS             complement(5102..>6531)
                     /gene="sdgB"
                     /locus_tag="AC1LW8_002181"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine-aspartate repeat adhesin
                     O-glycosyltransferase SdgB"
                     /protein_id="HIP4773986.1"
                     /translation="NRLNLFKEMGRPAQCVFLSWNRYLYRSAQNYITSSDYINMYDFF
                     QEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYRILD
                     YVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKDG
                     KPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESI
                     PVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDIEKRINHTI
                     PVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIEL
                     HMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALL
                     ESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSK
                     GSLAKAQQYSKASLIKQWDQFVRLI"
BASE COUNT         2537 a         1022 c         1108 g         1864 t
ORIGIN      
        1 attaacaggg ataataaaaa ggcaataaca aaaaagggta tgatttcaaa tcgcttaaac
       61 aaattttcga ttagaaagta tactgtagga actgcatcga ttttagtagg tacgacattg
      121 atttttggtc tagggaacca agaagctaaa gctgctgaaa acactagtac agaaaatgca
      181 aaacaagatg atgcaacgac tagtgataat aaagaagtag tgtcagaagc tgaaaataat
      241 tcgacaacag aaaatgattc aacaaatcca attaagaaag aaacaaatac tgattcacaa
      301 ccagaagcta aagaagaatc aactaaatca agtactcaac aacagcaaaa taacgttaca
      361 gctacaactg aaactaagcc tcaaaacatt gaaaaagaaa atgttaaacc ttcaactgat
      421 aaaaccgcga cagaagatac atctgttatt ttagaagaga agaaagcacc aaataataca
      481 aataacgatg taactacaaa accatctaca agtgaaattc aaacaaaacc aactacacct
      541 caagaatcta caaatattga aaattcacaa ccgcaaccaa cgccttcaaa agtagacaat
      601 caagttacag atgcaactaa tccaaaagaa ccagtaaatg tgtcaaaaga agaacttaaa
      661 aataatcctg agaaattaaa agaattggtt agaaatgata gcaatacaga tcattcaact
      721 aaaccagttg ctacagctcc aacaagtgtt gcaccaaaac gtgtaaacgc aaaaatgcgc
      781 tttgcagttg cacaaccagc agcagttgct tcaaacaatg taaatgattt aattaaagtg
      841 acgaagcaaa caatcaaagt tggcgatggt aaagataatg tggcagcagc gcatgacggt
      901 aaagatattg aatatgatac agagtttaca attgacaata aagtcaaaaa aggcgataca
      961 atgacgatta attatgataa gaatgtaatt ccttcggatt taacagataa aaatgatcct
     1021 atcgatatta ctgatccatc aggagaggtc attgctaaag gaacatttga taaagcaact
     1081 aagcaaatca catatacatt tacagactat gtagataaat atgaagatat aaaatcacgc
     1141 ttaactctat attcgtatat tgataaaaaa acagttccaa atgagacaag tttgaattta
     1201 acatttgcta cagcaggtaa agaaacaagc caaaatgtca ctgttgatta tcaagatcca
     1261 atggtccatg gtgattcaaa cattcaatct atctttacaa aattagatga agataagcaa
     1321 actattgaac aacaaattta tgttaaccca ttgaaaaaat cagcaaccaa cactaaagtt
     1381 gatatagctg gtagtcaagt agatgattat ggaaatatta aactaggaaa tggtagcacc
     1441 attattgacc aaaatacaga aataaaggtt tataaagtta actctgatca acaattgcct
     1501 caaagtaata gaatctatga ttttagtcaa tacgaagatg taacaagtca atttgataat
     1561 aaaaaatcat ttagtaataa tgtagcaaca ttggattttg gtgatattaa ttcagcctat
     1621 attatcaaag ttgttagtaa atatacacct acatcagatg gcgaactaga tattgcccaa
     1681 ggtactagta tgagaacaac tgataaatat ggttattata attatgcagg atattcaaac
     1741 ttcatcgtaa cttctaatga ctctggtggt ggcgacggta ctgttaaacc tgaagaaaag
     1801 ttatacaaaa ttggtgacta tgtatgggaa gacgttgata aagacggtgt tcaaggtaca
     1861 gattcaaaag aaaaaccaat ggcaaacgtt ttagttacat taacttaccc ggacggtact
     1921 acaaaatcag taagaacaga tgctaaaggt cattacgaat tcggtggttt gaaagacgga
     1981 gaaacttata cagttaaatt cgaaacgcca actggatatc ttccaacaaa agtaaatgga
     2041 acaactgatg gtgaaaaaga ctcaaatggt agttctgtaa ctgttaaaat taatggtaaa
     2101 gatgatatgt ctttagacac tggtttttat aaagaaccta aatataatct tggtgactat
     2161 gtatgggaag atactaataa agatggtatc caagatgcaa atgagccagg aatcaaagat
     2221 gttaaggtta cattaaaaga tagtactgga aaagttattg gtacaactac tactgatgcc
     2281 tcgggtaaat ataaatttac agatttagat aatggtaatt atactgttga attcgaaaca
     2341 ccagcaggtt acacgccaac ggttaaaaac actacagctg aagataaaga ttccaatggt
     2401 ttaacaacaa caggtgtcat taaagatgca gataatatga cattagacag tggtttctat
     2461 aaaacaccaa aatacagttt aggtgattat gtttggtacg acggtaataa agatggcaaa
     2521 caagactcaa ctgaaaaagg tattaaagat gtgacagtta cattgcaaaa cgaaaaaggc
     2581 gaagtaattg gaacaactaa aacagatgaa aatggtaaat atcgtttcga taatttagat
     2641 agcggtaaat acaaagttat ttttgaaaag cctgctggct taacacaaac agttacaaat
     2701 acaactgaag atgataaaga tgcagatggt ggcgaagttg acgtaacaat tacggatcat
     2761 gatgatttca cacttgataa cggatacttc gaagaagata catcagacag tgattcagac
     2821 tcagacagcg attcagactc agatagcgat tcagactcag aaagcgattc agattcagac
     2881 agcgactcag attcagatag tgattcagat tcagatagcg actcagactc agatagcgac
     2941 tcagattcgg acagcgattc agactcagat agcgactcag attcagacag cgattcagac
     3001 tcagatagcg actcagattc agacagtgac tcagactcag atagcgactc agactcagac
     3061 agtgactcag actcagacag cgattcagat tcagatagcg actcagactc agatagcgac
     3121 tcagattcgg acagcgactc agactcagat agcgactcag actcagacag tgattcagac
     3181 tcagatagcg attcggactc ggatgcagga aaacatacac ctgttaaacc aatgagtact
     3241 actaaagacc atcacaataa agcaaaagca ttaccagaaa caggtagtga aaataacggc
     3301 tcaaataacg caacgttatt tggtggatta tttgcagcat taggttcatt attgttattc
     3361 ggtcgtcgca aaaaacaaaa caaataatac aatatgaccc aggtccttgt ggcctggttt
     3421 ttttataatt acacatgcaa tagatgtatt tttcatataa aataaacaat aaagatacgg
     3481 aataaaactt atatgaggcg ataatatgaa ttacatttta ggaacaattt tagaaagtaa
     3541 aattacaggt gtagaaaaag cgcaaataaa tagattgaag ttgttcaaac aacacggcat
     3601 atcttcaaaa tgtgtatatg ttaaatggaa tccttattca tacacatatg cgaagcaaca
     3661 tcagattgaa aatgatgtat ttacaatgta tgactatttt caaaaagcaa tcaattataa
     3721 aaagacaaag caagttaact ggatacagta ttgggaaaag tcatgtaggt acacattgaa
     3781 atttgtggaa aattcaaatg atgtcagaat atatgatgaa gagcaattta taatgtatgc
     3841 tcatttttta gataaacagt atcatcattt aaactatgtg aattattttg atcataaaag
     3901 aagaaaagta aaacgcgaat tgtatgatgg aagaggcttt ttaagttgtt ctcgaatttt
     3961 aggtgaagga caacggattg tactcgaaaa ttactataca cctaatgggg aaatcgtcat
     4021 ccaaaaatat ttcgacgata taaaagggaa aaacacgctc acaaaggtta tcttaaatga
     4081 agatcagcat caacaatttt ttgatacaga agatgaatta gttcaatatt ttctccatca
     4141 attatgtaaa aataatgatc aaatcatatt agatcgtcct catgaattag gaaatgttat
     4201 agcgggatta aatcaaagta ttccagttat tgttgtgctc cacagtacac atttagccga
     4261 caccggtaat ggtataaaaa gtttttataa aacagtgttt aataatttaa cacgttataa
     4321 agcgattgtt gtatcaacag aacagcaatg ccaagatatt tcacaatata ttgaaaataa
     4381 aataccagtt atcaatattc cggttggcta cgtggcaaat ttaaagtatc aatttgacat
     4441 caatcaaaag gagaaaaatc atatcatatc aattgctcgc ctcgttgaaa ataaacaaat
     4501 taaacatcaa gttgaagtga tcaagcaatt agtaacaaaa catcccaata ttcaattgaa
     4561 tatttatgga catggaaatg gtttgtcaga atatcgacaa cttgtagaag attatcattt
     4621 atcggaacat gtaaaatttc atggttttaa gacgcatatt aatgaagaga ttgctaaagc
     4681 agaactgatg ttatcgacaa gtaaaatgga aggttttggc ttagcaattt tagagtcgct
     4741 ttcagtaggt acaccagtga tcagttatga tgtagattat gggccatcag aactgattca
     4801 agatggattt aatggctatt tagtacctca aggtgacatc aatcaaatgg ttgaaaaggt
     4861 cgaccaatta ctaaataata ctcaaaaatt gcaacagttt tcgattaata gcatagaatc
     4921 tgcacaacag tacaatgcaa ctactatcag tacaaagtgg caaaatattt taaactaagt
     4981 caaaagaaaa aagcattttc ctagaatcat ctatttaaca aacctcgttc atacgaactt
     5041 acatgtttag ttaaatgaca taggaaaatg ctatttttgt aaaaataaag gtaaagtaca
     5101 tttatataag acgaacaaat tggtcccatt gtttaattaa cgacgcttta ctatattgtt
     5161 gcgcttttgc taaactacct tttgacagtc gttgttgtac ttcaggatga tcaatcacat
     5221 attttacttt atcaaatagg gcatcttcat catttttagt aattaaataa ccattgaaat
     5281 caggcgtaat caattcgtta ggtccatatt taatatcata actaataact ggaacaccat
     5341 gtgctaaaga ttcaagtagc gctaaagaga aaccttccat gttacttgtt attaaactca
     5401 aataggcatc gctatattct tggtctagat tgcttaaaaa gccgcgtaag taaacatgat
     5461 tttccaatcc atatttttgt atcaattcat ttaatttttt actttcagaa ccaaaaccat
     5521 acatatgaag ctctattttt gggacgtacg atactaagcg tttaattaat tcaatttgtt
     5581 gatgtaattg tttttcaggt gaataacgag caacggaaat taatttaaca ctgcgctgat
     5641 ctaatgtttg gactggtgta tcaattgttt cactatagcc gacaggaata ttaacaactg
     5701 gaatagtatg gttaatacgt ttttcaatat ctaatttttg ctgctcagta gaaacgataa
     5761 ttgcacgata tcgagataaa ttttcaaaca tcgctttata tacattttta aatggcgatg
     5821 aatctaatgc atcaatattt ttaatgtgtg tactgtgaag cacagctact actgggattg
     5881 actcaggcgt taagttgaaa ataggtgctg tgtacacatt acgatcactg aaaaataaat
     5941 ccccatgttg atatagttgt ttaatgaaaa atgcgcctaa ttccgtttca ttattaaaga
     6001 aatattgttt gttagcatag taaacaataa ttttttgtac ttctggtttg ccatccttgt
     6061 aagaaaaata cttttctaat tttgtgtcac cttctggatt atagaaaaat tcacataatg
     6121 tttgttgttt atcaacaaga atcctactac aacttaaaaa gccacgcaca tcataaaaat
     6181 cacgttttac ttttcgtctt tgactatcaa aatgattcac ataatctaat atacgatatt
     6241 taggatcttg aaaatgggca tacattaaga aacgctcttg atcatatatt ctaaagtcat
     6301 gactattttc aacatgtttt aaagtataat gacattcatc agtccaatac gacaaccagt
     6361 caaatggttc attgcgttct aaatatgttg cttcttggaa gaaatcatac atattaatat
     6421 agtcagaact agtaatataa ttttgggcac ttctatataa atatctattc catgacagaa
     6481 atacacattg cgctggtctt cccatttctt taaataaatt taaacgatta a
//
LOCUS       DBNENW010000136         6485 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNENW010000136 DBNENW010000000
VERSION     DBNENW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.138"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            29..994
                     /gene="mraY"
                     /locus_tag="AC1LW8_002182"
     CDS             29..994
                     /gene="mraY"
                     /locus_tag="AC1LW8_002182"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIP4773987.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVVIIFVDQANPIILLLFMTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            996..2345
                     /gene="murD"
                     /locus_tag="AC1LW8_002183"
     CDS             996..2345
                     /gene="murD"
                     /locus_tag="AC1LW8_002183"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIP4773988.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIIYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            2361..3680
                     /locus_tag="AC1LW8_002184"
     CDS             2361..3680
                     /locus_tag="AC1LW8_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIP4773989.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQSNDENNDSASSRANNNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAFDNEQPQSAPKEQNGDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            3786..5198
                     /gene="ftsA"
                     /locus_tag="AC1LW8_002185"
     CDS             3786..5198
                     /gene="ftsA"
                     /locus_tag="AC1LW8_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIP4773990.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDSEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            5231..6403
                     /gene="ftsZ"
                     /locus_tag="AC1LW8_002186"
     CDS             5231..6403
                     /gene="ftsZ"
                     /locus_tag="AC1LW8_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIP4773991.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         2368 a          873 c         1264 g         1980 t
ORIGIN      
        1 aagtctggat taagaagagg agatagttat gatttttgta tatgcgttat tagcgttagt
       61 gattacattt gttttggtac ctgttttaat acctacatta aaaaggatga aatttggtca
      121 aagtattcga gaagaaggtc cgcaaagcca tatgaagaag actggtacac caacgatggg
      181 tggactaaca tttctattaa gtattgtgat aacgtctttg gtggttatta tatttgtgga
      241 tcaagctaat ccaatcatac tgttattatt tatgacgatt ggttttgggt taattggttt
      301 tatagatgat tatattattg ttgttaaaaa gaataaccaa ggtttaacaa gtaaacagaa
      361 gtttttggcg caaattggta ttgcgattat tttctttgtt ttaagtaatg tgtttcattt
      421 ggtgaatttt tctacgagca tacatattcc atttacgaat gtagcaatcc cactatcatt
      481 tgcatatgtt attttcattg ttttttggca agtaggtttt tctaatgcgg taaatttaac
      541 agatggttta gatggattag caactggact gtcaattatc ggatttacaa tgtatgccat
      601 catgagcttt gtgttaggag aaacggcaat tggtattttc tgtatcatta tgttgtttgc
      661 acttttagga tttttaccat ataacattaa ccctgctaaa gtgtttatgg gagatacagg
      721 tagcttagct ttaggtggta tatttgctac gatttcaatc atgcttaatc aggaattatc
      781 attaattttt ataggtttag tattcgtaat tgaaacctta tctgttatgt tacaagtcgc
      841 tagctttaaa ttgactggaa agcgtatatt taaaatgagt ccgattcatc atcattttga
      901 attgatagga tggagcgaat ggaaagtagt tacagtattt tgggctgttg gtctgatttc
      961 aggtttaatc ggtttatgga ttggagtgca ttaagatgct taattataca gggttagaaa
     1021 ataaaaatgt attagttgtc ggtttggcaa aaagtggtta tgaagcagct aaattattaa
     1081 gtaaattagg tgcgaatgta actgtcaatg atggaaaaga cttatcacaa gatgctcatg
     1141 caaaagattt agaatctatg ggcatttctg ttgtaagtgg aagtcatcca ttaacgttgc
     1201 ttgataataa tccaataatt gttaaaaatc ctggaatacc ttatacagta tctattattg
     1261 atgaagcagt gaaacgaggt ttgaaaattt taacagaagt tgagttaagt tatctaatct
     1321 ctgaagcacc aatcatagct gtaacgggta caaatggtaa aacgacagtt acttctctaa
     1381 ttggagatat gtttaaaaaa agtcgcttaa ctggaagatt atccggcaat attggttatg
     1441 ttgcatctaa agtagcacaa gaagtaaagc ctacagatta tttagttaca gagttgtcgt
     1501 cattccagtt acttggaatc gaaaagtata aaccacacat tgctataatt actaacattt
     1561 attcggcgca tctagattac catgaaaatt tagaaaacta tcaaaatgct aaaaagcaaa
     1621 tatataaaaa tcaaacggaa gaagattatt tgatttgtaa ttatcatcaa agacaagtga
     1681 tagagtcgga agaattaaaa gctaagacat tgtatttctc aactcaacaa gaagttgatg
     1741 gtatttatat taaagatggt tttatcattt ataaaggtgt tcgtattatt aacactgaag
     1801 atctagtatt gcctggtgaa cataatttag aaaatatatt agcagctgtg cttgcttgta
     1861 ttttagctgg tgtacctatt aaagcaatta ttgatagttt aactacattt tcaggaatag
     1921 agcatagatt gcaatatgtt ggtactaata gaactaataa atattataat gattccaaag
     1981 caacaaacac gctagcaaca cagtttgcct taaattcatt taatcaacca atcatttggt
     2041 tatgtggtgg tttggatcga gggaatgaat ttgacgaact cattccttat atggaaaatg
     2101 ttcgcgcgat ggttgtattc ggacaaacga aagctaagtt tgctaaacta ggtaatagtc
     2161 aagggaaatc ggtcattgaa gcgaacaatg tcgaagacgc tgttgataaa gtacaagata
     2221 ttatagaacc aaatgatgtt gtattattgt cacctgcttg tgcgagttgg gatcaatata
     2281 gtacttttga agagcgtgga gagaaattta ttgaaagatt ccgtgcccat ttaccatctt
     2341 attaaagggt gtgagtattg atggatgata aaacgaagaa cgatcaacaa gaatcaaatg
     2401 aagataaaga tgaattagaa ttatttacga ggaatacatc taagaaaaga cggcaaagaa
     2461 aaagatcaaa ggctacacat ttttctaatc aaaataaaga tgatacatct caacaagctg
     2521 attttgatga agaaatttac ttgataaata aagacttcaa aaaagaacaa agcaatgatg
     2581 aaaataatga ttctgcttct agtcgtgcaa ataataataa tatcgatgat tctacagact
     2641 ctaatattga aaatgaggat tatagatata atcaagaaat tgacgaccaa aatgaatcga
     2701 atggaattgc attcgacaac gaacaacctc aatcagctcc taaagaacaa aatggcgact
     2761 cgaatgatga ggaaacagta acgaaaaaag aacgaaaaag taaagtaaca caattaaagc
     2821 cattaacact tgaagaaaag cggaagttaa gacgtaagcg acaaaaacga atccaataca
     2881 gtgttattac aatattggta ttgttgattg ctgttatatt aatttacatg ttttcaccac
     2941 ttagtaaaat tgcgcatgta aatataaatg gaaataatca cgttagtact tcaaagataa
     3001 acaaagtttt aggtgttaaa aatgattcaa ggatgtatac gtttagtaaa aaaaatgcta
     3061 ttaatgatct cgaagagaat ccattaatca aaagtgttga gatacacaag caattaccaa
     3121 acacattaaa cgtagatatc acagaaaatg aaattattgc tttagtgaaa tataaaggta
     3181 aatatttacc tttattagaa aatggtaaat tgcttaaagg ttcaaatgat gtcaaaatta
     3241 atgatgcacc tgtcatggat ggtttcaaag gtacaaaaga agatgatatg attaaggcgt
     3301 tatctgaaat gacacctgaa gttagacgat atattgccga agtgacatac gccccaagta
     3361 aaaacaaaca aagcagaatt gaattgttta cgacagatgg acttcaagta atcggtgata
     3421 tttcgacgat atctaagaaa atgaaatatt atccgcagat gtcacaatca ttatcaaggg
     3481 atagttcggg taaactaaaa acacgaggct atattgattt atcagtcggt gcttcattta
     3541 tcccataccg tggaaacacg tctagtcaat cagaaagcga taaaaatgtg actaaatcat
     3601 ctcaagagga aaatcaagca aaagaagaat tacaaagcgt tttaaacaaa attaacaaac
     3661 aatcaagtaa gaataattaa aaaattttga tattgtctat gtttatagtt cacaagccat
     3721 tcaacgtatt gtaaactaag gatagtgtat ttttttaata gtaatttgtc aggaggtgcc
     3781 tatctatgga agaacattac tacgtaagta ttgatattgg atcatcaagc gtaaaaacaa
     3841 tagtaggcga gaaatttcac aatggtataa atgtgatagg tacaggacaa acctacacga
     3901 gcggtataaa aaatggttta attgatgatt ttgatattgc gcgacaagca atcaaagaca
     3961 caattaaaaa ggcatcaatc gcttcgggtg ttgatattaa agaagttttc ctgaaattac
     4021 ctatcattgg aacggaagtt tatgatgaat caaatgaaat cgacttttat gaggatacag
     4081 aaatcaacgg ttcacatatc gaaaaagtat tagaaggtat tagagaaaaa aatgatgtgc
     4141 aagaaacaga agtaattaat gtgttcccga ttcgttttat agtcgataaa gaaaatgagg
     4201 tttcagaccc taaagaatta attgccagac attcattaaa ggttgaagca ggcgtaattg
     4261 ctattcaaaa atcgatttta attaatatga ttaaatgcgt agaagcatgt ggtgttgatg
     4321 tattagatgt ttactctgat gcatataact atggttcaat cctaacagct actgaaaaag
     4381 agttaggtgc atgtgtcatt gatattggtg aagacgttac gcaagttgct ttttatgaac
     4441 gcggtgaatt agtagatgct gattctatcg aaatggcagg gcgtgatatt acagacgata
     4501 ttgcacaagg attaaacact tcttatgaaa ctgctgaaaa agttaaacac caatatggtc
     4561 atgcattcta tgattctgct tcagatcaag atatcttcac tgttgaacag gttgatagtg
     4621 atgaaacagt acagtatact caaaaagatt tgagtgactt tattgaagcg cgtgtagaag
     4681 aaatattctt cgaagtattt gatgttttac aagatttagg attaacaaaa gtaaatggtg
     4741 ggtttattgt aactggtgga tctgcaaact tacttggcgt aaaagaatta ttatcagata
     4801 tggtaagtga aaaagttaga attcacacgc catcacaaat gggaattaga aaacctgaat
     4861 tttcttcagc aatttctaca atttctagta gtatcgcttt tgatgagtta ttagattatg
     4921 ttacaattaa ttatcatgat agtgaagaaa ctgaagaaga tgttattgat gtgaaagaca
     4981 aagataacga atctaaatta ggcggatttg attggtttaa acgtaaaaca aacaaaaaag
     5041 atactcatga aaatgaagta gagtcaacag atgaagaaat ttatcaatca gaagataatc
     5101 atcaggaaca taaacagaat catgaacatg ttcaagacaa agataaagat aaagaagaaa
     5161 gtaaattcaa aaaactaatg aaatctctat ttgaatgatt attggccaat aaaactagga
     5221 ggaaatttaa atgttagaat ttgaacaagg atttaatcat ttagcgactt taaaggtcat
     5281 tggtgtaggt ggtggcggta acaacgccgt aaaccgaatg attgaccacg gaatgaataa
     5341 tgttgaattt atcgctatca acacagacgg tcaagcttta aacttatcta aagctgaatc
     5401 taaaatccaa atcggtgaaa aattaacacg tggtttagga gcaggagcta atcctgaaat
     5461 cggtaaaaaa gctgcagagg aatctcgtga acaaattgaa gatgcaatcc aaggtgcaga
     5521 catggtattt gttacttctg gtatgggtgg cggaactggt actggtgcag caccagtcgt
     5581 tgctaaaatt gcaaaagaaa tgggcgcatt aactgttggt gttgtaactc gtccatttag
     5641 ttttgaagga cgtaaacgtc aaactcaagc tgctgctgga gtagaagcta tgaaagctgc
     5701 agtagataca ttaatcgtta taccaaatga ccgtttatta gatatcgttg acaaatctac
     5761 gccaatgatg gaagcattta aagaagctga taacgtgtta cgccaaggtg tacaaggtat
     5821 ctcagactta atcgctgttt ctggtgaagt aaacttagac tttgcagacg ttaagacaat
     5881 tatgtctaac caaggttctg cattaatggg tattggtgtt tcttctggtg aaaatagagc
     5941 ggtagaagct gctaaaaaag caatctcttc tccattactt gaaacatcta tcgttggtgc
     6001 acaaggtgtg cttatgaata ttactggtgg cgagtcattg tcattatttg aagcacaaga
     6061 ggctgctgat attgtccaag atgctgcaga tgaagacgtt aatatgattt tcggtacagt
     6121 tattaatcct gaattacaag atgagattgt tgtaacagtt attgcaactg gttttgatga
     6181 caaaccaaca tcacatggtc gtaaatctgg tagcactgga ttcggaacaa gcgtaaatac
     6241 ttctagcaat gcaacttcta aagatgaatc attcacttca aattcatcaa atgcacaagc
     6301 aactgatagt gtaagtgaaa gaacacatac aactaaagaa gatgatattc ctagcttcat
     6361 tagaaataga gaagaaagac gttcaagaag aacaagacgt taatcggtta atatatatac
     6421 acaaataatt caacacaaat catcagataa catatctgat gattttttta ctaattttta
     6481 gaaca
//
LOCUS       DBNENW010000137         6443 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNENW010000137 DBNENW010000000
VERSION     DBNENW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.139"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            52..552
                     /gene="ftnA"
                     /locus_tag="AC1LW8_002187"
     CDS             52..552
                     /gene="ftnA"
                     /locus_tag="AC1LW8_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HIP4773992.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(634..726)
                     /locus_tag="AC1LW8_002188"
     CDS             complement(634..726)
                     /locus_tag="AC1LW8_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4773993.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            942..1496
                     /locus_tag="AC1LW8_002189"
     CDS             942..1496
                     /locus_tag="AC1LW8_002189"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIP4773994.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(1563..2633)
                     /gene="dinB"
                     /locus_tag="AC1LW8_002190"
     CDS             complement(1563..2633)
                     /gene="dinB"
                     /locus_tag="AC1LW8_002190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIP4773995.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(2880..3410)
                     /locus_tag="AC1LW8_002191"
     CDS             complement(2880..3410)
                     /locus_tag="AC1LW8_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HIP4773996.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPAQ"
     gene            complement(3580..4941)
                     /gene="rlmD"
                     /locus_tag="AC1LW8_002192"
     CDS             complement(3580..4941)
                     /gene="rlmD"
                     /locus_tag="AC1LW8_002192"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIP4773997.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(5022..5969)
                     /locus_tag="AC1LW8_002193"
     CDS             complement(5022..5969)
                     /locus_tag="AC1LW8_002193"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIP4773998.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         2098 a         1158 c          902 g         2285 t
ORIGIN      
        1 actaagttta attcatagta attttaattt acaaataaga ggtgtatcaa aatgttaagt
       61 aaaaatttat tagaagcatt aaatgatcaa atgaaccatg agtactttgc agcacacgca
      121 tatatggcaa tggcagcata ctgtgataaa gaatcgtacg aaggatttgc aaacttcttc
      181 attcaacaag ctaaagaaga acgtttccat ggacaaaaga tttataacta tattaacgac
      241 agaggtgcac atgcagaatt cagagcagtt tcagcaccaa aaattgactt ttcaagcata
      301 ctagaaactt tcaaagacag cttatctcaa gaacaagaag taacaagacg tttctataac
      361 ttatctgaaa tcgctcgtca agataaagat tatgcaacta tctcattctt aaactggttc
      421 ttagatgaac aagtcgaaga agaatcaatg tttgaaactc acatcaatta tttaactcgt
      481 atcggcgatg acagcaatgc attatatctt tacgaaaaag aacttggcgc tcgtacattc
      541 gacgaagaat aattaaacat cactacaata gacagataaa tatcatacga catgataggc
      601 atttgggtca cttacaataa cccaatgtct atattatttt gctttacgga gatcactaga
      661 ttcattttct gaatcattga tctgcgtttt ttcattttca aggctaatta ttgtattttt
      721 agtcatttat tttttaaact actaatgtta ataactctaa atttgatgtt gaattaattt
      781 gaccgatttt aaagcatatc atcatttact ttttaatcag agttacatcc aaatgataga
      841 tttcacgtta taccttcacg tataatatta tgtatcgttt gtaagcaaat gactaaaagt
      901 ctattaatat atacatttaa ttaattgaaa ggattgacta catgatacaa gatgcgtttg
      961 ttgcacttga ttttgaaaca gcaaatggta aacgtacaag tatttgttct gtcggaatgg
     1021 ttaaagtcat tgatagtcaa ataacagaaa catttcatac tcttgtgaat ccgcaagact
     1081 atttttcaca acaaaatatt aaagttcatg gcatacaacc agaagatgtt gaaaatgcac
     1141 ctacgtttga ctacgtattt ccatatatga tgcaatttat tgcagattta cctgttgtcg
     1201 cacataacgc ggcatttgat atgaacgtct tacatcaaag cattcaaaat attggtttac
     1261 caacaccaaa tttaacttac ttttgtagtt atcaacttgc taaaagaacc gttgattcgt
     1321 atcgatacgg tttaaaacat atgatggagt tttatcaatt agattttcat ggtcatcatg
     1381 atgcattgaa tgatgccaaa gcatgcgcaa tgattacttt taggctactg aaaaattatg
     1441 aaaatttaac atatgtaact aatatttatg gtaaaaatct aaaagataaa ggctaggact
     1501 aaataaaata ctcccttcaa agtaagcatt gtaaaaatgt aaactttgca gggagcttta
     1561 ttttatataa agtcatatat cgtcatattt ttataagttg attgttctaa attacctaca
     1621 gtgacaccaa taagtcgaat tggtacatca gggtctttta aatcgttata aagtaaatat
     1681 gcaatattat aaatatcttc ttcagaacta accgaatctc ttaaactcat ctgtttagat
     1741 agcgtttcaa attgataagt tttaatttta accgttacag ttttagctga cttctgtaat
     1801 ttatttagac gttcagctgt tttacctgac aattcccata cttttcttaa aatctcttca
     1861 tcatcattca cgtctgttgc aaatgtgcgt tcagtcccta ctgattttct tactcttgat
     1921 gatttcactt cactatggtc aataccgcgt gccttgttat ataaaccccg acctcttttt
     1981 ccaaacaaac gtattaattc aaattccgtt ttctcatata aatctctacc gttaaaaata
     2041 ccattatcat gcattacttt tttggaagct ttacctacgc ctggaaaatc tccaatatcc
     2101 aatgtcatca aaatatcatg gacattttga taatcaatca cagtcatacc atcaggttta
     2161 ttcataccac tcgctaattt agctaaaaat ttgttataag aaacacctgc agatgctgtt
     2221 aaatgtgtct gctctagaat atcttttcta atatactgag caattttcga agcaggaagg
     2281 tctggtctca ctaattctgt aatatctaaa tacgcttcat ccaatgacat cggttctacc
     2341 ttatctgtat aacttcggaa aatagacata atctgcgcag atgtttctcg gtaagcacca
     2401 aaattacttg tgacaaagta tccatttgga cataatttat gcgcttgtga cataggcatt
     2461 gctgaatgga cgccgtattt tcgtgcttca taagatgccg tagagacaac acccctactg
     2521 cttgctttac caccaacaat aactggtttc cctttcaatt tggggttgtc tctcatttcg
     2581 acttgtgcaa aaaaatagtc catatctata tgaataattc gtctctcagt caagtgctca
     2641 cctccctact aatttttact tttataacgc acaaaaatat ctcaacataa ttatacgctg
     2701 tgtacgattt ttttacataa atcttgcact tagcgataac tatattgaga taactacaag
     2761 ttgttataaa atcaattgct atttaagcat gatgatgaag acgattgagt aagaaaacat
     2821 aggtaatctg aaataattca agcaaattca ttttgttggt atcatcatat taaaatttat
     2881 tattgagccg gcttttgatg atacaaataa atactatctt catcttgttg aacatattta
     2941 aaactactag atattgttaa tgcaacaagc agaatatcta taaagcaata agcagtatgc
     3001 atactaaaca taaaaataag tgatgaataa ccaaatacct taattaaaat aagcaagcca
     3061 gtacttaata ggattagtgg tgacagcata ataattgaga attgccattt gttgaagcaa
     3121 gcatctgctg tttggaataa gattctgtct ttttttatat taaacatagg tttgctatct
     3181 tttttaaata aaagaaataa tgctctatgg ataagttcat gtaaaatcaa taaaataatg
     3241 aatccagcaa acccatatac aagattgatg atgatatttt gatcgacaac cgctgtgaca
     3301 cctaacgccc acttatacgt aaataaaatc acgaataacg caataacaag ttgcaagata
     3361 ataaaccttc gcatttgaaa attatttgtc gttaaatcaa ttttatgcat taccaaccct
     3421 cccgatcatg acattcttat tcttctttaa atatagtata caatgtcaca tttaatttaa
     3481 aaagttcata tcaagaaagt aaattggctg taataaaatt ttaatatacg acttctttct
     3541 tcacttatta aggcgaaatt ttatctcaaa tcatgtgcgc tatttcaaat tgaataatgc
     3601 cactgtctca acatgtgttg tttgtggaaa catatctacc ggtgttacct cttcaagttg
     3661 atatttttca gctaataata atgcatcacg ttgctgtgtt gcgggattac atgaaatata
     3721 gacaatacgc ttaggttcta atgtaagcaa agtctgaata aacgtttcgt cacagccctt
     3781 tcttggcgga tcaaccatta caacatctgg tttaatccct tgtgctttcc attgtaaaat
     3841 aacttcttca gctttcccac agacaaaagt tgtattattg cattggttta tagtcgcatt
     3901 ttgttgtgcg tcttcaattg cagaaggtac tacttcaaca ccgtatacat gttttgcatg
     3961 tggtgccata tatagcccta ttgttccaat accacaatag gtatctaata caacttcatt
     4021 acctgtcaat tgcgcatatt caattgcttt attatataat ttctctgttt gttcagaatt
     4081 aatttggtag aatgactgat cacttatttt aaatgtacta tctgttaatt gatcaataat
     4141 tgtatcttta ccatatagcg ttatagattg acgccccata ataacattag agtggctatc
     4201 attaatgttt tgtttaatgc ttgtcacatt aggaaatgca tctaatatct tctcaacaac
     4261 agcatttttt tgtggccact ttttaccatt agttacaaaa ataatcatca tttcgtctgt
     4321 atgatatcct gttcttacaa ccaaatgtct cattaaacct tttttcaatt gttcttgata
     4381 aatacttaca tttaaatctt ttaaaataga tttaacttca ttcatcactt cttgatgttg
     4441 tgaatcttgt attaaacaac tttccatgtc aataatgtca tggcttcttt gacgataaaa
     4501 gcccataata acttcattct gttcattctt accaactgga atctgggact tgtttcgata
     4561 tctccaagga tctgtcatgc caactgtatc gttaatctta gaattatcaa aatgcgcttt
     4621 tcgctgaaac aaattaatca cttgttcctt tttcatttca agttgtgctt cgtatgataa
     4681 gtgttgaagt tggcacccac cacaacgttc ataatatata catggcggtg caacacgatt
     4741 ctcacttctc gtatttatat taagcaactt acctatcgca aagttctttt ttactttgat
     4801 aattttatat tcaatttgtt cattaattaa agcttgtggt atgaaaatag gaaagcgatc
     4861 tatttttacg acaccatggc cttcatgcgt taaatcaaca actgttcccg tttttatgtc
     4921 atttttagct attgcttgca aaattttacc tccaaaatga acaggttagg aacaaaatta
     4981 tgcgcttcct aacctgccat tatatatttc actatttctg tttattcttc gattaaattg
     5041 tcatcaacat gatcattatt tattaactct tcatttacaa tatcattagg tgcaaagaca
     5101 tctatatgac gttctagatt taagaaattc gctggtaatt taccaccata ttctccatct
     5161 acatttagtt gtaagtctgt gaatgatgaa atattaattg cctttgcttt ttcataaata
     5221 actttaggat gcttagtatg ttctcctctt gaagctaaag tcataatatg accaagttct
     5281 gcaaggtttg atttttcaac tataattaac gtaaaatagc cgtcatctaa cttagcgtcc
     5341 ggcactaatt tttcaaatcc tgccattgaa tttgttaaac ctaaaaagaa taataatgct
     5401 tctccttgga aaacattacc atcatattca attcttaaat ctacagcttt catttgaggt
     5461 aacatttcga aacctttgat gtaataagca aatggaccaa caatagattt caatttactc
     5521 ggtgtttcat aagagacttg cgtcaattgt ccgcctgcag ctaaattaat aaagtatcga
     5581 ttattcattt taccaatatc tactttagta gaatgacctt caatgatgac atcaagtgcc
     5641 cccatgatgt cattaggtat atgcaatgca cgtccaaagt cattaacagt acccatagga
     5701 atgacaccta gcttaggacg attaggcttt tctgcgatac cattaactac ttcatttaat
     5761 gttccatcac cacctgcagc gattaataca tcataatttt catgcatagc tctttctgct
     5821 tcaagtgtgg catcacctat tttctcggtt gcatatgcac tcgtttcata tcccgctttt
     5881 tctaatttta ttaaggcatc aggtaattct cttttaaata gctctttacc tgatgtcggg
     5941 ttataaatga ttctagcacg tttcctcata tcttatccct ctacttaaaa ttcatatatt
     6001 ttaacatcat ctttgtttcg tctaataggg agtgggacag aaataatatt taacaaaatt
     6061 tatttcgttc taccccaact tgcacattat tgtaagctga cagaaagtca gcttctttgt
     6121 tggggcccca ctagaattga aaaaagcttg ttacaagcgt attttctttc agtcaactac
     6181 tgccaatata acattgtagt gcctaggaca ttgaatttat gacccaggct cagtcttatt
     6241 tcatcattct taatatcgtt aaagaccaac ttgtatctta aacaaatact atctcaatat
     6301 gtacaaagct tgttatttat tcagcatttt ttgccgttct tcattatata gacttcgtca
     6361 gttatgctat tttaccttta aaatgatgtt gtaaatataa tgttgtcttt atacaaaaat
     6421 gttttatacc tttttattat aca
//
LOCUS       DBNENW010000138         6397 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNENW010000138 DBNENW010000000
VERSION     DBNENW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.140"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            384..698
                     /locus_tag="AC1LW8_002194"
     CDS             384..698
                     /locus_tag="AC1LW8_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIP4773999.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            698..1990
                     /gene="arsB"
                     /locus_tag="AC1LW8_002195"
     CDS             698..1990
                     /gene="arsB"
                     /locus_tag="AC1LW8_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015105 - arsenite transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HIP4774000.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHIGFIRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNALNIKQV
                     IKGAPWNIVLFSIGMYIVLFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            complement(2077..2931)
                     /locus_tag="AC1LW8_002196"
     CDS             complement(2077..2931)
                     /locus_tag="AC1LW8_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HIP4774001.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3207..3431)
                     /locus_tag="AC1LW8_002197"
     CDS             complement(3207..3431)
                     /locus_tag="AC1LW8_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774002.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3630..4100
                     /gene="sigS"
                     /locus_tag="AC1LW8_002198"
     CDS             3630..4100
                     /gene="sigS"
                     /locus_tag="AC1LW8_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HIP4774003.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            4213..4656
                     /locus_tag="AC1LW8_002199"
     CDS             4213..4656
                     /locus_tag="AC1LW8_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HIP4774004.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(4643..5086)
                     /locus_tag="AC1LW8_002200"
     CDS             complement(4643..5086)
                     /locus_tag="AC1LW8_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774005.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
     gene            5382..6017
                     /gene="sdpA"
                     /locus_tag="AC1LW8_002201"
     CDS             5382..6017
                     /gene="sdpA"
                     /locus_tag="AC1LW8_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpA"
                     /protein_id="HIP4774006.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGIIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
BASE COUNT         2145 a         1001 c          827 g         2424 t
ORIGIN      
        1 catttaatat caaccttagt gtttgtgtta aaatattttt aacgaggtga attaaattga
       61 aatttatagc aaagatatta atctcattag taattctatt tatgattttt agtcgtaaaa
      121 acaaagcttc ttaatcaata cccttgatta caaaataaat tcaactaagg tgattggcca
      181 tacactcaag atatggctaa tcaccttttt caatgcaaaa caaagagtat attttgcaaa
      241 atcaatatct ataatttatc tttaataccc ttttaatact aaaaccaaat ttaaattaca
      301 tagtcatatc gtgtaatata ccaaaggaac atagacattc atctatatag atgataatct
      361 atttaacgtc taggaggtat gttatgacgt ataaagaact agcaacattt ttaaaagttt
      421 tatcagattc aagcagatta gaaatactag atttactttc ttgtggagag ttatgcgctt
      481 gtgatttgtt agcacatttt caattctctc aacctacact tagctatcat atgaaagcat
      541 tagtaaaaac caacttagtt acgacacgaa aaatcggaaa taaacattta taccagctta
      601 atcataatat ttttgagtcc gtaattaata acttgtcaaa ggttcatacc tctaaccaac
      661 gatgtatttg tcataacctt aagactggtg aatgctaatg atgacaactt tagcgacact
      721 catttttcta gtaactttat tatttgtatt atggcaacct aaaggcctag atattggcat
      781 taccgcatta actggtgcct ttattgctgt tattactggt gttgtaagtt tttccgatgt
      841 tttcgaagta acaggtattg tttggaatgc tactttgact tttgtctcag tcattcttat
      901 ttcattaata ttagataaag ttggattatt cgaatggtca gctattcaca tgcttcatgc
      961 ttcaaaaggc aatggtttaa aaatgttcgt ttatatcata ttattgggtg ccattgttgc
     1021 tgcatttttc gcaaatgatg gcgcagcgtt aatcttaacg cctattgtat tagcgatggt
     1081 taaaaatata ggttttagta agcgggccat attccctttt attattgcga gtggttttat
     1141 agctgacaca acttctttac ctttgatcgt gagcaatcta gtgaatatta tatctgctga
     1201 ttattttcat ataggattca ttcgatattt tagtagaatg attataccta atttattctc
     1261 acttttagca agtattatag tattgtggtt atattttaga aaggcgatac ctaaaacgtt
     1321 tgatgataat aatataaagc atcctaaaga tgccattaat gatttaaagc tatttaaaat
     1381 ttcatggatt gtgctagtta tattactttt cggctatctt atcagtgaat ttactaaaat
     1441 tccggtatca attttcactg gaatcattgc ttttattttt ctaatgttgg ctcgtaaatc
     1501 aaatgcttta aatattaagc aagtcattaa gggcgcacct tggaatatag tattattttc
     1561 aattggtatg tatatcgtct tattcggctt aagaaatgct ggcattactt taatattggc
     1621 taaaatatta gaatatattt ccaattacgg tctatttagc actattttgg gaatgggctt
     1681 catttcagcg tttttatcat caataatgaa taatatgcct acagttttaa tagatgcgat
     1741 tgctattggt caatcaaatg tccatggcat gttaaaagaa ggcctaattt atgcgaatgt
     1801 tatcggttct gatttaggtc caaaaattac accgataggc tctctagcta cattactgtg
     1861 gttacacgtc ttaacacaaa aagatgttaa gatttcttgg ggcacatact ttaaaaccgg
     1921 tatcatcatt acaattccag tactatttat aaccctcata gggttgtatc taacacttat
     1981 catattttaa ttaataaaaa gacgcttttt atttattgcg atagaaagcg tctttttata
     2041 cttgagcaag ttatatcata acttaactct atcaaattac ttattcaaat gtttactgtc
     2101 atctttatac acaaagtatt tgaagtattt tccttcagtc ttcatgttct tataaaagtc
     2161 agcgataatt gttgcattac tttctgccca cttaatatca gtagcatatt gatgttctcc
     2221 tggatttttt ggattccatc tcatactata caatgtattt tgatctgtgc ttgataagaa
     2281 gtgcttatga atgaaatcag caccgcctga aatagctttt tcaggtgtat cccaaccatg
     2341 ctttttagca tattctgcac ctgttttaat tgggtcttta tcaagggctc ctactccata
     2401 gaaattgtag tattttttgc catcaatttc gactccatta gctaattcac ttttaactgc
     2461 gccagtttct aataatgcat gtgaaattaa ataaacttcg ttaacgtgct tatctttagc
     2521 agcttttaag aaatcatccg tatgtttcaa taacgttggt ctatctacta acatacgttt
     2581 aattctattt ttatcaatcc cttgatactt tgataaatct aaaaattgat atttttgctt
     2641 ttcattatcg ataaaagtac cgctatccat tgcactttta atttcagttg cagatgcatc
     2701 tctccatgca tcattctttt tatttgatac ctgttgactc gtataattat ttatttgttt
     2761 ctttgctgca tcgtttaatg taacatttaa cttttcaatc ttaatgtcgg atttaacatg
     2821 tttgaaaaat atctgatcgg atatcattga gaaaaataaa aatgagacaa cagcaaatat
     2881 gacaacaagt cctattattc caaaaataga acctttcttg tgtttattca tatccacacc
     2941 tcttaggtca ttgttgttat acattcagtt tgattacgat aataaagttt aacattatta
     3001 aaaccgttta acaattttat ctatgcattg ttacatattc ataaataaac agtaatgtaa
     3061 atgaaacgtt ttaaaccatt tatcaacatt actttacgtt gatttacgaa tcataaatgt
     3121 tttttctttt tgtataacct ttgaaaacct ttatatcaat aatcttatta ttcacgtatt
     3181 aaaaaacaaa aaattctctt gttcgtttac ttagtttctt ttttataatc catcataata
     3241 aaacttgctg catttgatct atttttataa tagtactgat ttaaatcaat cgtaccttca
     3301 atttgaccat cacgatacat catttcatta gtgaagttat ttatcatcac gaaatcagcg
     3361 cgatctctta taacatctaa atcgtcgttt cgtgcaaaaa aatgttctaa atttaaatgt
     3421 gcgtaatcca ttgttacctc ccgattacaa attttgttct atctactatt tagcagagta
     3481 ataatacatc atatcacatt aaattaatta cttatgtctt atatttaaca cttaatttag
     3541 cgattacgca aatgaatgat ttttctaaat attttattct aactttatcc ggaaatattt
     3601 gttatacgta taacttggag aatgactact tgaaatttaa tgacgtatac aacaaacacc
     3661 acaaaatcat acaccatctt ttaaaaaaat ataatattag ctataattat gatgagtatt
     3721 atcaactact cttgataaaa atgtggcaat tgagtcagat atataaaccc tcaagcaagc
     3781 aatctttatc ctctttttta ttcactcgat taaattttta ccttatcgat ttattcagac
     3841 aacaaaatca attaaaagat gtcattttat gtgagaataa ttcaccaaca ttaactgaac
     3901 aaccaacata ctttaatgaa catgaccttc gtttacaaga tatcttcaag cttttaaatc
     3961 aaagagaaag actatggctc aaactatacc ttgaaggata caagcaattt gaaattgctg
     4021 aaatcatgtc attatcgctt tcaacgatta aattaattaa gatgtccgtt aagcgtaaat
     4081 gccaacataa ttttaattag tttattgtga aatttcggtc gatgtgttaa tgatgttgaa
     4141 acaccctaac atcttcatat aaatttattt taaccattaa attaacaact tattatacga
     4201 ggagtactat atttgcaaga caattctact aaatatctac tttatatcca aactgctact
     4261 tcaaaccatc tcgaaacaaa ttgtgtcttt ttacattgcg attacattct taaagttcca
     4321 attaacaaac tcgtttcata ttatgcgaaa ttgcatttat catcacaaag tgtgctaatt
     4381 gagactgcaa aaaacatact aaatattaat aaactggttc ctatttacat caacccaaaa
     4441 actatacttt ttccgttaaa acataaacgt gcaccgatac aaatttatat caatgcacat
     4501 tatattgttg gtatgactgc catagagaat tcaacattaa tacattttca agaaggcatt
     4561 cagctagaag ttgatgagcc attttctcta gtttcaaaaa aatgtcatga aagtttagct
     4621 ttgaagcact tcattgaaaa tactatttcg aattaaattt aatttctaaa agtttcttag
     4681 taaagtatga tgtcacaatt tttataaaat atatgctaac aatcattaat aaaatcgctc
     4741 caataattgt taaaataaca acagttgttg ataaatctac cttaggacta acgaaaaatt
     4801 gtacgccaga tagacttgta attagcccaa ataaaaacaa gcctaataaa gcaacataag
     4861 caaacatcat cactactaat gtaacaaaca tactataact ttttgtttct ttatttcgaa
     4921 atacattttg tcttaagtaa gcattagcaa aggtaacagg cttatttaag ttaacgctgt
     4981 catcatcaga tatagaagac aatcgttgat ctaatgcttg tttctccttc ttgttcaaaa
     5041 accataattg ctttttaact tttttatcaa atgatgcttt attcataatt aaggatttct
     5101 ctcctttata atattgacgt aattttaaac attagcgtca attgaaagtg tattttatat
     5161 attttacaga aatatatgta tcaaatctac aaaaatccat attacaattt catttatggt
     5221 gatttgacgc taaaatacag tctgctaatt ttccaaacta atgcttttta ggtaggtaca
     5281 atgataaatt ttaatcgtat atatgtttca tatataatga atctatcctt aaacatattg
     5341 cattcaaaat aattttaaaa aatgaaaaga aaggatttag catgcaaaaa ttcaaagact
     5401 ttttttacga tgatttatcg gttacacgag gaaattattt tttaacttta atggcagcat
     5461 tttttattac tatcatttta tttatcggca tagttgtcag tgaagtacat ttactttata
     5521 gcatgctaat tgtattagta ggtttaattc tattgaggct attcaaaatc aatttattct
     5581 cttttaaaaa attaacattg tctcaagtta tttatattat aggcggtgca ctattaattt
     5641 atgggttaga taatctttat ttatattttc atgacgtacc ggcaaatgaa caacaattag
     5701 agcaagaaat acgaaataca ccattctata tttctatttt cactgttacc atcatccccg
     5761 ctattgtgga agaaattgtt tttcgcggta tgataataag ggttatcttc agaaaacact
     5821 tgtttttagg gttaattgtg tctagtttag tttttgcatc attacacgaa tctgacactt
     5881 ggattggtta tttaccttac ttatattctg gtttgatttt tggtataatt tatataaaaa
     5941 caaaacgatt agaagtggta atattcatgc acttcttaaa taacttgtta gctctgctct
     6001 ttataatatg gggataattt tgagataacc tttcatatta ttttcaaatt atctatctga
     6061 tatttatttt attgaacttg agtcaccact taaactcaaa aatacgtgtc atcaagtgca
     6121 aaataattta gaactaatac aaacataaaa acttttgctt tacgcttaaa atcaaaagtc
     6181 acatacctag atataagtac aacttgtata ttttgtactc taataggtat gtggcttaaa
     6241 taatatttaa ttcttaagtt gcatatagta attgttttta ttcattttct aaaatatgcg
     6301 agactaattt ccttttctac tcctgttgaa tcaatttaga cttttttata actcgagctt
     6361 ttacttagct gaattttgac ttcttcttca ttaataa
//
LOCUS       DBNENW010000139         6360 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNENW010000139 DBNENW010000000
VERSION     DBNENW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.141"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            25..972
                     /locus_tag="AC1LW8_002202"
     CDS             25..972
                     /locus_tag="AC1LW8_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP4774007.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            1574..4528
                     /gene="fdhF"
                     /locus_tag="AC1LW8_002203"
     CDS             1574..4528
                     /gene="fdhF"
                     /locus_tag="AC1LW8_002203"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIP4774008.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            4528..5001
                     /locus_tag="AC1LW8_002204"
     CDS             4528..5001
                     /locus_tag="AC1LW8_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HIP4774009.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            5229..5462
                     /locus_tag="AC1LW8_002205"
     CDS             5229..5462
                     /locus_tag="AC1LW8_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774010.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            5484..6260
                     /locus_tag="AC1LW8_002206"
     CDS             5484..6260
                     /locus_tag="AC1LW8_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HIP4774011.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
BASE COUNT         2304 a          826 c         1283 g         1947 t
ORIGIN      
        1 aggaatagta aaggagtggt gggattgaaa cgttcgtcta aaagtaaaat gagcctaccg
       61 aaaaaaatat ttttatgggt attcggtatt ttagtaatct tagcgattgt agctgttgtg
      121 tatgtagctg ctaaaatttt tattactggt aataagatac acaatccgtt agatcgcaat
      181 cattcagaat taagagataa aaaagtgagt ttaaacgatg gtgatccatt cacaatcgct
      241 ttatttggtg tcgattcaga tgctgatcgt aagaaaaagg gtggcggaga gcgtagtgat
      301 agtattatga ttttatctat caaccctaaa acgaagaaaa ctgaaatagt aagtataccg
      361 cgtgatacaa gagcggaaat tgttggccgt ggtactactg aaaaaattgc acatgcctat
      421 gcatatggtg gaccaaatat ggctgttaaa tcacttgaaa aattaatgaa tgtgccaatt
      481 gatcattatg cgacaatcga tatggatggt ttgcataata tgatagatag catcggcggt
      541 gttgatgtag taagtaatga tacattcact gttgatggtg ttcgttttac aaaaggacag
      601 cagacacatg taaatggtga tcaagcattg aagtttatta gaagtcgtaa agaagaaggt
      661 gctggtggtg actttggtcg acaacaacgt cagcaaattg tacttgaagc gatggctaat
      721 aagattgcaa gtccatcatc gattacacat tttaatagtc taatgaatga aattcaaaat
      781 aatgttaaaa cagatttaac attaggtgat ttgaatacga ttagaagcaa ttataaagat
      841 gctaatgaca cgattaataa acatcaattg agtggtcaag gcggcattca aagtgacgga
      901 ttgtactact tcattcctag tgagcagtca aaagctgaaa gtactaagtt attaaaagat
      961 aatctagagt agtaaactaa gggaaaataa gttatcaaaa ttgtacacaa attaattaga
     1021 tagaatgatc tgtaaataga ataacgaata gaactaatag cccgtaaata gaaggtgata
     1081 acactttgta tttatgggct attttattta atgcctgatt tgatttttat aaattaatcc
     1141 attacaaatt taatttaaag ataaatctta ccttctaaat gatagtagat atttatcaaa
     1201 cttgggttat taattttcag aaaaagggta tttataaata gtataacgat ttttaggtga
     1261 ggtgattatt aaatattgtc attaataagg ttgtgaaaga tatggcaaaa agcgtgaaac
     1321 agataattgg aaaactaata atgataatta ttttcaatta aacaaaaaga tgatttaatt
     1381 tcgcgcaaaa tatttcaaat aaaaatgatt gtaaaaaggc aaaatacaaa atttcactta
     1441 acaactagta cataaagtaa tacaattaaa ttaattctat ctgaaagatg tgtggggcat
     1501 cgttatttta ggtggatatg agcaatttat taaaagtcat ttacggaaaa tatatataga
     1561 cggggtgagt aatatgcaag aacatttggt ggttacactt gatggaaaag attatcttgt
     1621 agaaccaggt acgaatttac ttgaatttat taaatcacaa gatacttttg taccttcaat
     1681 ttgttataac gagtcgatgg gaccaattca aacatgtgat acatgtactg ttgaaattga
     1741 cggtaaaatt gaacgttcat gtagtacggt gattgatcgt ccaatgactg taaatactgt
     1801 gaacaatgat gtgaaagatg ctcaaaaaga agcgcttgat cgaattttag aaaagcatat
     1861 gctgtattgt acagtatgtg attataataa tggtgattgt gaaattcata atacgatgga
     1921 tgcatggggg cttcagcatc aaacgtatga atataaagag aagccatacg aaaaagatta
     1981 cggcccattt tatcgttatg atccaaatca atgtatctta tgtgggcgtt gtgtagaagc
     2041 atgtcaagat atcgaagtga atgaaactat tagaattgat tgggatcgtg aacatccacg
     2101 tgttatttgg gataatgatg taccgattaa tgagtcttca tgtgtatctt gtggtcaatg
     2161 tgcgacggta tgtccatgta atgccatgat ggaagtgaat atggaaggta atgcgggtta
     2221 tatgactgat acggaacctg gttcattagc agcaatgatt gatttaacta aaaaagcaga
     2281 acctggttat ggtccactat ttgcaatctc agattcagaa gcagaaatgc gtaaagagcg
     2341 tattaagaaa actaaaacag tgtgtactta ttgtggtgtg ggttgttcat ttgaagtttg
     2401 gactaaagat agagaaattt taaaagtaca accatcacat gattctccag caaataaaat
     2461 tgcgacttgt gttaaaggta agttttcatg gggacatatt aattcagatc aacgattaac
     2521 taaaccacta gtaaggaaaa atggtgagtt ccatgaagta gaatgggatg aagctttaaa
     2581 tgtcattgca gataatttta catctattaa agaaaagtat ggcccagatg cactatcatt
     2641 catttcttct tctaaagcga cgaatgaaga atcgtattta atgcaaaaat tagcaagaca
     2701 agttattgga acaaataacg ttgataactg ttcaagatat tgccaagcac ctgcaacaaa
     2761 aggcttattt agaacggttg gacacggcgg tgactcaggt agtattgaag atctagaaaa
     2821 agcggcaatg tctgtattga taggtaccaa tacagccgaa gcacacccag ttattgcatc
     2881 acgcatgaaa cgtgctcaaa aattatttgg acaaaaaata catgtgtttg atattagaaa
     2941 acatgaaatg gctgaacgtg cggatcgttt ttatcaaccg aaaccaggta cggatttagc
     3001 gtggttaagc gcagtgacta agtatattat tgatcatgat ttacacgata aagcatttat
     3061 tgatgagtgg gtagatgatt ttgatgaata ttacaaatca ttagaaacat ttacaatggc
     3121 ttttgctgaa gaagcaacag gtattcctga atcagaattg attaaatttg ctgaagaatg
     3181 tgctaaagct gaatctgttg taatttgttg ggcaatgggt attacacaac aagacattgg
     3241 tagtgactca agtacagcga tttcaaactt attattagta acaggtaatt atcgtcgtcc
     3301 tggtactggt gcgtatccat tacgtggaca taataatgtt caaggatgta gtgatatggg
     3361 aagtatgcct gataagatta ctggttatca aagtattgaa gcggatgata ttcgcgctaa
     3421 atttgaaaaa gaatatggcg ttaaattgaa tccaaaagct ggtaaagata atcatgaaat
     3481 ggtagaaggt atacatgacg gagaagtaca ctcattgtac ttatatggtg aagatacggg
     3541 tattgtagat tcaaatatta attttgtaca agctgcgttt gaaaaattag atttcatggt
     3601 agtccaagat gaatttttaa cattcacagc aacatacgca gatgttgtat tgccagcaag
     3661 tccttcactt gaaaaagacg gtacttttac aaataccgaa cgtcgtattc aacgtttata
     3721 ccaagcatta gaacctcttg gtgattcaaa acctgactgg aaaattttcc aagcaattgc
     3781 taatagatta ggatttgatt ggaattacaa gcatcctagt gaaattatgg atgaagtagc
     3841 acgcttaaca cctctatatg ctggggtaag ttatgatcgt ttagaaggat tcaatagttt
     3901 acaatggcca gtacaacctg atggtactga tgagcctatt ctatacttag aaggattcaa
     3961 ttttgataat ggtaaagcaa aattattccc attatcattt gataattact ttaagcaaga
     4021 tgaaatttat gatattcatg taaacaatgg tcgattgtta gagcacttcc atgaaggtaa
     4081 tatgacttat caaacaccaa tgattaaata caaagtgcca cgtgcatttg ttgaaatttc
     4141 tccagagctt gctgaagata gaggcattca tgaaggtgca gaagttaagc tgatttctga
     4201 aacaggagaa gcggtattac aagttcacgt gacagatcgt gttaaaggta aagaaatcta
     4261 tattccatta aataatgatg cgatggaaaa tggagatcta ggtgcaatta acttattaac
     4321 taatagtgat gttgatcaat acacggatac accatcttat aaacgtacta gctgtcgttt
     4381 ggaggtaatt acgaaacgtg gtaagtcacc gttgaaccct aataatttcc gtgtcaataa
     4441 aaaacgtcat ccgcagtaca gtgttcaagt acagaaaaaa tgggaacgtt cagattatgt
     4501 tttcccggga aatcaggtgg ataaataatg gctgagagaa tatcttctaa aattagacgc
     4561 ttagaaaaat cagaagaaca aattaagtta gaaagtttaa atgaagttac agaggcaatt
     4621 gcagcgaata aagatagtat attaaaagca atcaagctta ttaaaacatt agatgatgca
     4681 aaactattag atgctttaaa tggtgcaatt cgaggacgtc aagtcatcat aaataaattt
     4741 gcagttgaac ttaataaaga tatttataca gggctattat ctaatatggc ttcaatggta
     4801 tttttattag gtgaactcaa tgtatcagat ttaagcgact tcctaaataa agttaataaa
     4861 ggattacacg ttgccaatca agcgagtcca aatgctaaga caactataag aagtctattg
     4921 ggcgtattga aagatgacga catgaaccgt agtttaacat acatgcttaa tatgctaaaa
     4981 ggtatgtctc gagaagaata acattacaat gaaaaagtgt ttctacatgc aagcagtgta
     5041 ggagcacttt ttttaatttt tgtaaaaatt aaaaagggtg tttttgatgg aataaatgaa
     5101 taatattgaa aaacaaaaaa atcacgtgct attgtgaatc aatcatcgac aggcctatga
     5161 aacatcatgt attattatga ttatgtaagt tatttaaata aaaagggtga gtgcatatta
     5221 attcgagaat gttaaaaacg tcagctgtaa tttctaatac attattagtc ataggtttag
     5281 tctgtctatt tatgatgaaa ttagtgttag ctattacttt ttttgctgtt tcattatcaa
     5341 ttagtctagt tgtctttaat atgatgttca gagatagaac aacgatgaaa gtaatcgtta
     5401 acgcttcttt tttaatcgta atattagcga ttgtagtggc atatttttta ttgtctaagt
     5461 aagaaaaatg aggttttgtc tatatgaaga agaatttcaa gttacgcatt tcaacgctac
     5521 tattgatagt tattttagtt gtttttgctg tattactcat cgtgaatgaa actaaattgt
     5581 ttaaaaatga tgtgaattac tcttttgatg aggctgtttc aatgcaacaa gggaaaggta
     5641 ttgtacagac aaaagaagag gatggtaaat ttgttgaagc aaataataat gaaattgcta
     5701 aagcaatgac tatttcacat aaagacaatg atatgaagta tatggatatc acagaaaaag
     5761 tgccaatgtc ggaatctgaa gttaaccaat tgctaaaagg taaggggatt ttagaaaatc
     5821 gagggaaagt ttttctagaa gctcaagaaa aatatgaggt taatgtcatt tatcttgtta
     5881 gccatgcatt agtagaaaca ggtaacggca aatcagaatt agcaaaaggc attaaagatg
     5941 gaaaaaaacg ctattacaac ttttttggta taggagcatt cgatagtagt gctgttcgta
     6001 gtgggaaaag ttatgctgaa aaggaacaat ggacatcacc agataaggcg attattggtg
     6061 gtgcaaagtt cattcgtaat gaatattttg aaaacaatca actgaattta tatcaaatgc
     6121 gatggaatcc agaaaatcct gcgcaacatc aatatgcgag tgacattcgc tgggcagata
     6181 aaattgccaa attaatggat aaatcctata agcagtttgg tataaagaaa gatgatatta
     6241 gacaaacata ttataaataa gacatcggtg cttaaaggag ctggaacaat ttattgtttc
     6301 gagctccttt agcacattct gagtgtgtta gttaaatgga ttttaaccta acaaaaaacg
//
LOCUS       DBNENW010000140         6299 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNENW010000140 DBNENW010000000
VERSION     DBNENW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.142"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(277..1740)
                     /locus_tag="AC1LW8_002207"
     CDS             complement(277..1740)
                     /locus_tag="AC1LW8_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIP4774012.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(1758..6257)
                     /gene="gltB"
                     /locus_tag="AC1LW8_002208"
                     /pseudo
     CDS             complement(1758..6257)
                     /gene="gltB"
                     /locus_tag="AC1LW8_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
BASE COUNT         1830 a         1444 c          922 g         2103 t
ORIGIN      
        1 attcctccta aacacaatat ttatcatata tgaacgttct gtaaaagtca atattgtttt
       61 taagcattac atgattacca caaatcattg tcttccacta cagttgacct ataaacgaat
      121 gtaaaaaatc actacttata attaatcgat ttgttgtctt acaaaccttt gtttttaggt
      181 tacaaagatg gcatgtgacg ttgatagata ctagattatt tcaaactcga ttcagcatgc
      241 cacaacgtca acgtaaccac catttccata caaagattat acacaaactt tactcgctaa
      301 atactgatct actgctttcg ctacgcctct accttcttta atcgcccata caactaaact
      361 ttgaccacgt ctagcatctc cagcagcaaa taccttttca ttattagttt gatagtttgt
      421 atcatccgcc acgattcgat ttctatccgt tttaatgtta aaagcattcg gtactgttgg
      481 ttctgtacct tcgaagccga ttgataataa tacaaggtca gcaggccaaa atctttcagg
      541 tccttctttc atgaccatac cattttcgcc ttgctctaaa atttgagtat acaaaccacg
      601 tatgtgtcct ttatcgtcaa catcgtaacg cattgtttga acaccatatg cacgtggttc
      661 cttaccaaac ttagcttcgt actcttggtg cgcatagtcc attttaaaca ccggcattgc
      721 taaaggccat gatgcatttt ctgtaaatgt aattgcttct ggcaatttcg tatatttatt
      781 aaattgaaca atcgatttac aattttctct taatgctgtc gctacacagt ctgcccctgt
      841 atcaccagca ccaatgataa tgacattctt atcttttgca gttattgtta tatcatcaat
      901 ttctccattt aacaactgcg tttgttcagt taaataatcc atagcgaaat gtataccatc
      961 acccatgcgt ccttctaaag gtaaatctct acctttttgt gcaccagtac ataatataat
     1021 ggcatcatac tcagattcta acgttgcttt atcaatatca acgcctactt caacaccatt
     1081 aatgaaagta atgcccgctt cttccattaa cttaatacga cgtcgaacca catctttatc
     1141 aagtttcata ttcggaatac catacattaa taaaccgcct gattctctag cacgttcata
     1201 aatagttact tgatatccta gtagattaag ttcttcagca gcagctaatc ctgctggacc
     1261 gcttccaacg attgccactt tttcatctct acggcgactc ggaactttcg gcgctaccca
     1321 accattttca aaagcttcat caataattgt gcgttcaata cctttaatcg caatcgattc
     1381 tctattaatc ttcatcacac aagcactttc gcatggtgca ggacatacac gccctgtaaa
     1441 gtcaggaaag ttatttgttt cgcttaagcg ttcataagca gttttaaaat cttgatgata
     1501 cactaagtcg ttccattcag gaatgtagtt tccaattgga caacctattg tttccctacc
     1561 atactgttgt ccggtttgac aaaacggcgt tccacaatcc atacatcgtg caccttggat
     1621 agaggcatct tctttagtaa atcgttgttg atatgcttta tgatgcttca aacggtctac
     1681 cagtgataat tcacctaagt actgtttgtc atacttcata aatcctttaa attcacccat
     1741 cgtatctccc cctttcctta atacacaacg gctggtttat gtttagcgtc gattgtttta
     1801 ctgtcatcgt aaaatgcagc taacatcgct tcatcttcat tgtcatgtaa tgatttgtgc
     1861 aaatgaattt tttgcatcat taattgataa tctttaggaa taactttaac gacgacatct
     1921 tcaatgcgat caaaatgttt taacacatga atcgctctcg tactattcgt gtgtgacaca
     1981 tgttcttcca gcatttgctt aatgaatgct ttttcttctt ggtgtttaat ctttgtaaac
     2041 gaaagcgtat ctagttgatt attttcaaca aaagcttcta catcagacgg gataacgtaa
     2101 gcaataccac cactcatacc ttgaccgaag ttcttaccta catcacctaa attaatgaca
     2161 tgtccaccag tcatgtactc taatccatgg tcaccgatac cttcaacgac aacatctaca
     2221 ccactatttc taatacagaa tctttctcct gcactaccgt taataaatgc cttaccacct
     2281 gtcgcaccat agaatgagac gttaccagca ataatttcat tttgtcgttc ttcaaaaggt
     2341 gctttgacaa tgaccgtacc accagataat cctttaccaa catagtcatt cgcatctcca
     2401 gtatgatgaa tcattaagcc tttcggtgca tatgctgcaa gactttgacc agcatgacca
     2461 ttcgtataaa cattaattgt attttcagga agtcctgctt ctccatattg tttcgaaatc
     2521 tcactacctg taataactcc tacatcacgt tgttcattat ttactgtaaa gctacctgta
     2581 tagcgacgcc cttcagcaat atacggcttc gttacttcat ataaatttgt taaatcaaat
     2641 ccatgctcaa gattatgatt ttgttgaatt tcttttgtgt ttggcccatc gaaaggacat
     2701 aacagttttt caacatcaat actagccgct ttgctattca cttttaatgt tgatgatcgt
     2761 tgtaataaat cagttcttcc aactaagtct tctacacgtt tcaaacctaa agatgctaaa
     2821 atttctctta attcttgtgc aataaaatgc ataaaattaa caacatgatg tgctttacct
     2881 ctatataaag cacgtaaatc tttgttttga gttgcaactc ctactggaca tgtatcttta
     2941 tggcatacac gcatcataat acagcccaac accactaatg gtgcagttgc aaatccaaat
     3001 tcttccgctc caagcgcaca tgcgtacgct acatctttac cagttaataa cttaccgtct
     3061 gtttctaact taacacgact tcttaagtcg tttagtttta atgtttgatg tgtttctgct
     3121 aaaccaatct cccaaggaac accggcatgc tgaatactcg ttttaggtga agcccctgta
     3181 ccaccatcgt aaccactgat gacaatttta tctgcaaatg cttttgccac cccagatgca
     3241 atggtaccaa cacctgtttt cgaaactaat tttaccgcga tatctgcatc tttattcgca
     3301 ttttttaaat catgtatcag ttgcgctaaa tcttatattg aataaatatc atgatgtggc
     3361 ggtggtgaaa tcagaccgat acctggcgtt gaccctcttg tcttcgcaat ccacggatat
     3421 accttagtac caggtaattg accaccttca ccaggctttg caccttgcgc aactttaatt
     3481 tgaatttctt tggcatgttg taaataatca ctagttacac caaaacgccc agaagcaact
     3541 tgtttaatcg cacttacttt gttgcttcca tcaacttgta cttcataacg ttttgcatct
     3601 tcgccacctt caccactatt actctttcca cctaattggt tcatggcttg tgctaacgtt
     3661 tcatgtgctt ccgctgaaat cgatccataa ctcatcgccc ctgtattaaa gcgtttgaca
     3721 atgtcactta ccggttcaac ttggtcgatg tcaatcggtg tacatgcttt aaattcaagt
     3781 aaatgtctaa tgtgatctgt tctatttttg ttcaccgctt cagagtatgc tttaaattgc
     3841 gcatagtcat tttctttaca tgcgtgctgc aataagaaaa tagattccgg attaaaagca
     3901 tgatgttgac cttgttgtct ccattggaat gtactacctg atgcaagata attatcatca
     3961 ctttgttgac gtgctttatt ttcagcatca atttgatcaa tcgaaatacc cgataactta
     4021 gactgtatcc cagtaaaata acgatcaatc acatcatgag acaagccaat cgcttcaaat
     4081 atttgtgcac cttgataact ttgcactgtc gaaattccca tcttagccat tactttaatg
     4141 acaccttcgg acaatacatc cgtatatgtc ttaacattat cgacaacggt gccttgtaac
     4201 ccttctgtca atgtcagttg ttcaactgta cgttgcgcta ggtatggcac aattgcattc
     4261 gcgccatatg cgagtaaaca agcaacatga tgcacttctc gtgtctcacc agatttagcg
     4321 actaaacttg tagacatacg taaatctgct ttaataagta attgatgcac atgacttatt
     4381 gcgagtaaca tcggcattgc aaagccattg ctatcaacta atccactatc atctaacact
     4441 agaatttgag cgccttgctt tacagcattc actgcttctc ggcctaatgc ttctaacgca
     4501 tcttccaaat ccccttcata taccgttgat aaataagtta atttaaaatg ttcctgatca
     4561 atcgctgcta tgtgtgattc attcaatacc ggccttttca attgaatacg atctaaaacc
     4621 gtttcgtcag gtgctagtaa gttaccttcg ccacctaaat aagaaagttc actcgttacg
     4681 attttttcac gatacgcatc aattggtgga ttcgtaactt gtgcaaacag ctgtttaaag
     4741 taattaaata gtgattctgg tcgctcgttc aacactgcaa ttggcgcatc atatcccatt
     4801 gcaccgatag gatccttctt accttctaca agttcctgaa tatacttatg aatctcttct
     4861 ttcgtgtatg caaactgacg ttgtaattta aataacgtct catctttcca ttgcgaatct
     4921 tgatattgta tattttcaaa atcaaagtca actttatggt tatcaatcca cgctttatat
     4981 ggtaattctc cagcaatcgc acctttcaaa tcattatttt caatgacttt attctgttta
     5041 aaatcaacaa gcaataactt tccaggattc aattgacctt taaaagcaac attactttca
     5101 ggtacgtcca caacacccac ttcagatgaa aagacaataa agttatcttt agtaatcgta
     5161 taacgacctg gacgtaatcc atttctatct gtaagcgcgc caagtttgtc accgttacag
     5221 aacgaaatca ttgtaggacc atcccacggt tccattaaat aactataaaa ttcataaaat
     5281 gcacgtacat ttgcatcatt cgcttcattg tataaccaag gttcaggtat gagtaacatc
     5341 gctgcctttt ctggctccat ggctaacgat aagaactcta gcgcattatc tacaatggca
     5401 gagtcactac catcctcatc gacaatttga aacactttat gttgatcctc gccaaataat
     5461 gtttcgatta atttatgttg gcgtgctcgc atccagttca cattaccttt aatcgtgtta
     5521 atctcaccat tatgcattaa catacggtta ggatgcgccc ttttccaact cgggaatgta
     5581 ttcgtactaa atctcgaatg cactaaccct agctttgatt gatataaatc atccgataaa
     5641 tctgtataga gttttttaat ttggtctgat cgtaaccaac ctttatatac aattgttttg
     5701 cgtgataagc tcgtaaaata caattctaaa tcgcactgag tcgaatagaa ctctaattgt
     5761 tttctcgcta aaaacaaacg cttttcaaca tcttcaatgt ccctaatatc aataaacact
     5821 tgttgaatga ctggcatcgt atctgctaca tgtttagcaa tggcatcttt attaactggt
     5881 acattacgat aaccaagaat tgataaccct tcaccttcaa aatatttttt aaaaactgct
     5941 tcatgttcag aacctaaaat gcgttctttg gaaaaaaata accccacggc atattcacct
     6001 tcacctggga tatcaaagtc cgttacatgt tgtttgaaaa atgcaaaagg tatttcagtc
     6061 ataatacctg cgccatcacc agtgatgcca tctgcgccga ccccgcccct gtgatctaag
     6121 cgtcgcaaca tttcaagcga tttatcaatg atgtcgtgag accttttatt atccatattc
     6181 gcataaaaac caataccaca cgcatcatgt tcctcacgat agtcatataa gcctttaatt
     6241 aatttctcat tgtgcatgat gcacacccct tttttagaat attctgatat tagttatta
//
LOCUS       DBNENW010000141         6270 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNENW010000141 DBNENW010000000
VERSION     DBNENW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.143"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            301..1593
                     /gene="obgE"
                     /locus_tag="AC1LW8_002209"
     CDS             301..1593
                     /gene="obgE"
                     /locus_tag="AC1LW8_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIP4774013.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1606..2061
                     /locus_tag="AC1LW8_002210"
     CDS             1606..2061
                     /locus_tag="AC1LW8_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIP4774014.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            2075..2677
                     /gene="ruvA"
                     /locus_tag="AC1LW8_002211"
     CDS             2075..2677
                     /gene="ruvA"
                     /locus_tag="AC1LW8_002211"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIP4774015.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2709..3713
                     /gene="ruvB"
                     /locus_tag="AC1LW8_002212"
     CDS             2709..3713
                     /gene="ruvB"
                     /locus_tag="AC1LW8_002212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIP4774016.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            3715..4740
                     /gene="queA"
                     /locus_tag="AC1LW8_002213"
     CDS             3715..4740
                     /gene="queA"
                     /locus_tag="AC1LW8_002213"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIP4774017.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4763..5902
                     /gene="tgt"
                     /locus_tag="AC1LW8_002214"
     CDS             4763..5902
                     /gene="tgt"
                     /locus_tag="AC1LW8_002214"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIP4774018.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5921..6181
                     /gene="yajC"
                     /locus_tag="AC1LW8_002215"
     CDS             5921..6181
                     /gene="yajC"
                     /locus_tag="AC1LW8_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIP4774019.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
BASE COUNT         2213 a          864 c         1299 g         1894 t
ORIGIN      
        1 aaaaagcaaa aaaagtatca tatttggtat tcggaaatat cagcaatttg agtaaagtaa
       61 atttaaaagc aactgcataa gagcccctaa tcgctaaact ataaggggac aaaagaatac
      121 agttgcagta taaaaagcaa aaaaagtatc atatttggta ctcggaaata tcagcaattt
      181 gagtaaagta aatttaaaag caactgcata agagccccta attatttaca atatataagg
      241 ggctctaata tgctataatt attgggaaaa tgaaaattat atgtaaaaga ggtgagatat
      301 atgtttgtcg atcaagtcaa aatatctctt aaagccggtg atggtggtaa tggtattacc
      361 gcatatagaa gagaaaaata tgtaccattt ggtggaccag ctggcggtga cggtggtaaa
      421 ggtgcttcag tcgtatttga agtggatgaa ggtttaagaa cgttattaga ttttagatat
      481 caacgtcatt ttaaagcaag caaaggtgaa aatggccaaa gtagtaatat gcatggtaaa
      541 aatgcggaag atttagtatt aaaagttcca cctggtacaa ttattaaaaa tgttgaaaca
      601 gacgaagtgt tagcagatct tgttgaagat ggtcaaagag ctgtagtagc gaagggcggt
      661 cgaggtggcc gaggtaattc acgttttgca acacctagaa accctgcacc tgacttcagt
      721 gaaaaaggtg aaccaggtga ggaattagat gtatctttag aattgaaatt attagctgat
      781 gtaggattag taggtttccc tagtgtgggt aaatcgactt tattatctat cgtttcaaaa
      841 gctaagccta aaattggggc atatcatttt acaacgatta aaccaaatct aggtgttgtt
      901 tcaacgcctg atcaacgtag ttttgttatg gcagatttac caggtttaat tgaaggtgca
      961 tctgatggcg ttggattagg acatcaattt ttaagacatg tagagagaac aaaagttatt
     1021 gttcacatga ttgatatgag cggttctgaa ggtagagaac ctattgaaga ttataaagtc
     1081 attaatcaag aattagctgc gtacgagcaa cgtttagaag atagacctca aatcgtagta
     1141 gctaacaaga tggatttacc tgaatcacaa gataatttaa acttgtttaa agaagaaatt
     1201 ggcgaagatg tgccagttat tccagtttca acaataacgc gtgataatat tgatcaatta
     1261 ttatatgcaa tagcagataa attagaagaa tataaagatg ttgacttcac agttgaagaa
     1321 gaggagtcag ttggcattaa ccgagtatta tataaacata caccgtcaca agataaattt
     1381 acaatttcaa gagatgatga tggtgcttat gtggtaagtg gtaatgctat tgaaagaatg
     1441 tttaaaatga ctgactttaa cagtgatcca gcagtacgtc gatttgctcg tcaaatgcgt
     1501 tcgatgggta ttgatgatgc acttagagaa cgtggttgta aaaatggtga tatcgttaga
     1561 attcttggcg gagaatttga attcgttgaa taggagcgta acatgatgga caataaagat
     1621 tataaaaagt tttatttaat tagagaagat gtcttgcctg aatccgtggt taaaacattg
     1681 aagattaaag atgccttaaa aagtgatccg acattgtcca tttatgatgc cgttaaacag
     1741 tttgatctat ctagaagtgc tttttataaa tatagagaaa cgatatttcc agtagacgat
     1801 aaaatgcttg accatcgaga atttacatta attttatatg taactgatat tgtgggtatg
     1861 ttggcacgtg tactagatgt tatatcaaag ttagaactat ctgtattaac gattcatcaa
     1921 agtattccaa tggaagaaaa agcaacaata acattatcac tgaatgctaa atctaaagaa
     1981 acttcagtag aagatgttat tggtgctttg agaaatttag attatgtatc aaaagtagaa
     2041 ttaattagta tgagtatgta aggatgtgcc tataatgtac gcgtatgtca aaggtaagtt
     2101 aacacattta tatcctacac acgtagttgt tgaaactgct ggtgttggtt atgaaattca
     2161 aacaccaaat tcttatcgtt ttcaaaagca tctagatcat gaagttttaa ttcgtacatc
     2221 tttaattgtt cgtgaagatg cacaattatt gtatggattt agtagtgaag aagagaaaga
     2281 tatgttcttg agtttaatta aagttactgg tattggtccg aaatcagctt tagctatttt
     2341 agcgacaagt acgcctaatg aagtaaaacg tgccattgaa aatgaaaatg atacgtattt
     2401 aactaaattc ccaggaattg gtaagaaaac ggcaagacag attgtcttag atttaaaagg
     2461 taaagtgaaa atcactgaag aagatagcga ttcattatta caagtagacg ctacttcgac
     2521 ggtgcaagat caattcgtgc aagaagcaat gttagcgtta gaagcattag gttattctaa
     2581 acgagagctt gcaaaagttg agaaaacgtt aaataaaaat aaatatgact cagttgatga
     2641 agctgttaag gcaggtcttc aattagttgt atcttaattt taaatagatt aataggggaa
     2701 gtgttgtcat gaatgagcgt atggttgatc aatcaatgca tagtgaagaa actgatttcg
     2761 aattgtcgct tagacctacg agattacgac aatatattgg tcaaaattca ataaaaagta
     2821 atttagaagt atttattaaa gcggctaaac ttcgtcatga accattagat catgtattgc
     2881 tttttggccc ccctggatta ggtaagacaa cattatctaa tatcattgcc aatgaaatgg
     2941 aagttaatat acgtacagta tcagggcctt cattagaaag acctggtgat ttggctgcaa
     3001 ttttatcagg acttcaacct ggagatgtat tgtttattga tgaaatacac agactgagta
     3061 gtgttgttga agaagtgtta taccctgcaa tggaagattt ctttttagat attatcattg
     3121 gtaaaggcga tgaggctaga agtatccgta tcgacttacc tccattcact ttggtaggtg
     3181 caacaacgcg agctggcagc ttaacaggtc cactaaggga tcgatttggt gtgcacttaa
     3241 gattagaata ttataatgaa tcagatttaa aagaaatcat tattagaaca gctgaggttt
     3301 taggcacagg tattgatgaa gaaagtgcca ttgaacttgc taaacgttct agagggactc
     3361 caagagtagc aaatcgacta ttgaagcggg taagagactt ccagcaagtg aatgaagatg
     3421 agcaaatata cattgaaaca acgaagcacg cattaggttt acttcaagtt gatcaacacg
     3481 gactagatta cattgatcat aaaatgatga actgtattat taagcagtat aatggtggac
     3541 ctgttggttt agatacgatt gccgtaacaa ttggtgaaga acgtattaca attgaggacg
     3601 tttatgagcc atttcttatt cagaaaggct ttttagaacg tacgccacgt ggcagaaaag
     3661 caacaccatt agcttatgaa cattttgcaa agtcgaatga ggagagagga taacgtgaat
     3721 attgaagaat ttgactatga cttaccagaa tcattaattg ctcaaacgcc tttaaaagat
     3781 cgtgatcata gtcgtttatt agtcatggat agagaaactg gtgaaatgaa acatttacat
     3841 ttcaaagata tcattgagta ttttagacct ggtgatacat tagtgcttaa cgatacgcga
     3901 gtaatgccag ctagactttt tggtttaaaa gaagaaactg gtgcaaaagt tgaaatgtta
     3961 atgttaactc aaattgaagg taatgattgg gaagtcttac tgaaaccagc taagcgtatt
     4021 aaagttggta ataaattgaa ttttggtaat ggcaaaatta tagctgagtg cataaaagaa
     4081 atggatcaag gtggacgcat catgcgttta cattatgaag gtattttaca agaaagatta
     4141 gatgaattag gggaaatgcc actgccacca tacatcaaag aacgtttaga tgatccagat
     4201 cgttatcaaa cagtttacgc taaagaaagt ggttcagcgg cagcaccaac agcaggatta
     4261 cattttactg atgagttatt aactgaaatt aaaaataaag gcgttaatat cgcatttgtt
     4321 acattacatg ttgggttagg tacgtttaga ccggtgagcg tcgatgatgt gaatgaccac
     4381 gaaatgcata gtgaatatta tcaaatgaca caagaaacag ctgatttatt aaatgatact
     4441 aagtccaaag gacatcgcat tatatcagtt ggtacaactt caacacgtac acttgaaaca
     4501 attcgacgcg atcatgataa atttgttgaa acgagtggct ggactaatat atttatttat
     4561 ccaggatttg attttaaagc aattgatggc cagattacta attttcattt accaaaatca
     4621 acattagtta tgctagtatc agcgtttagt actcgtgaaa atgttctgaa tgcttataaa
     4681 acagcagtaa atttagaata tagattcttt agttttggcg atgcaatgtt aattatataa
     4741 aaagaatgtg aggattttga atatgcctgc agtaacatac gaacacatta aaacttgtaa
     4801 acaatcaggt gcgcgtttag gtatcgtgca cacaccacac ggttcatttg aaacacctat
     4861 gtttatgcca gttggtacta aagcaaccgt taaaacaatg agtccagaag agttaagaca
     4921 aattgaagca aaaatcattt tgggcaacac atatcatttg tggttacaac ccggaaatga
     4981 tattatcaaa cacgctgggg gattacataa attcatgaat tgggatggtc cgattcttac
     5041 agattcaggc ggtttccaag tgtttagttt aagtaattta cgtaaaatta cagaagaagg
     5101 cgtggaattt agacatcata ctaatgggtc taaattattt ttgagtcctg agaaatcaat
     5161 gcaaattcaa aatgatttag gatctgatat tatgatggca tttgatgaat gtccaccgat
     5221 gcctgctgaa tatgattatg taaaaaaatc tattgaacgt acaacacgtt gggcgaaaag
     5281 atgtctagat gcacaccaaa gacctgaaga tcaagcattg ttcggcatta tacaaggtgg
     5341 cgaatatgaa gatttaagag aacaaagtgc aaaggattta gtagaattag attttcctgg
     5401 ttatgcaatc ggcggtttgt cagttggtga acctaaaccg gttatgtata aaatggttga
     5461 acatacagag cagtttatgc ctaaagataa accaagatat ttaatgggtg taggatctcc
     5521 agatgcgtta atcgaatgta gtattcgcgg catggatatg tttgattgtg tcttaccgac
     5581 acgtattgcc agaaatggta cttgtatgac atcgcaaggt cgtttagtta ttaaaaatgc
     5641 aaaatttgca gatgatttaa gaccgttaga tgagaattgt gactgttata catgtcaaaa
     5701 ctattcaaga gcgtatatac gtcatttaat caaggcagag gaaacttttg gtattcgtct
     5761 tactactatt cataatttac attttctgct aaaattaatg gaagatataa gacaagccat
     5821 tcgagaagat cgtcttttag atttcaaaga agaattcttc gagcaatatg gattaaatgt
     5881 tgagaaccca aaaaactttt aagcaagagg agcgtataaa atgcaatttt cattactaat
     5941 atatatagtc gtaatttttg cggttatgta tttcttgatg atcagaccac aacaaaaacg
     6001 tgcgaaacag catcgtgagt tgattaataa cattcaatct ggtcaaagaa ttacaactat
     6061 tggtggtatt aaaggtactg ttaaagcagt agatgaaaca actgttgtta ttacagttaa
     6121 tggtcatggt actgaattaa ctttcgaaaa acctgctatt aaacaagttg acccttcata
     6181 atgagaagtg gtattaaaaa ggatgaaacg aatagcgttt catccttttt aagttataag
     6241 tagtatgtag aatagcttat ttttattcca
//
LOCUS       DBNENW010000142         6270 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNENW010000142 DBNENW010000000
VERSION     DBNENW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.144"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(97..204)
                     /locus_tag="AC1LW8_002216"
     CDS             complement(97..204)
                     /locus_tag="AC1LW8_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774020.1"
                     /translation="MVLLTFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(241..564)
                     /gene="isdG"
                     /locus_tag="AC1LW8_002217"
     CDS             complement(241..564)
                     /gene="isdG"
                     /locus_tag="AC1LW8_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HIP4774021.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(583..1317)
                     /gene="srtB"
                     /locus_tag="AC1LW8_002218"
     CDS             complement(583..1317)
                     /gene="srtB"
                     /locus_tag="AC1LW8_002218"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIP4774022.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1379..2344)
                     /gene="isdF"
                     /locus_tag="AC1LW8_002219"
     CDS             complement(1379..2344)
                     /gene="isdF"
                     /locus_tag="AC1LW8_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HIP4774023.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLITKFTTG
                     NNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGV
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2357..3235)
                     /gene="isdE"
                     /locus_tag="AC1LW8_002220"
     CDS             complement(2357..3235)
                     /gene="isdE"
                     /locus_tag="AC1LW8_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HIP4774024.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3232..4308)
                     /gene="isdD"
                     /locus_tag="AC1LW8_002221"
     CDS             complement(3232..4308)
                     /gene="isdD"
                     /locus_tag="AC1LW8_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HIP4774025.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYILFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4308..4991)
                     /gene="isdC"
                     /locus_tag="AC1LW8_002222"
     CDS             complement(4308..4991)
                     /gene="isdC"
                     /locus_tag="AC1LW8_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0030492 - hemoglobin binding [Evidence
                     IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HIP4774026.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5200..6252
                     /gene="isdA"
                     /locus_tag="AC1LW8_002223"
     CDS             5200..6252
                     /gene="isdA"
                     /locus_tag="AC1LW8_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HIP4774027.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         2045 a         1112 c          802 g         2311 t
ORIGIN      
        1 tactataaat catacgcccc aaaatatcat tattaattct tttcttcttc aaaataaatc
       61 aaaatgatat aattgatgat tattttcaaa gcacattcaa atcaaactat gttttagcaa
      121 tttgttgtta gcatgtttgt gttcattaaa aaaacgacca tcatcggtat catgtatggt
      181 cgttacaaaa gttaacaata ccaattgtca taacaagtac tgcaacctct ttaaattcaa
      241 ttatttcatg taactatagc ctatatcata tgtaattact ttgttattga taatcgggct
      301 actttcatct tcatttttac ttctaacatg tttatgtgct gctttaaaga catcagattt
      361 taaccaatcc gtaaaagctt gctttgattt ccaaactgtt aaaattttca cttcatcaaa
      421 atcttcctgt tctaaagttt gtgtaacaaa catgccatca aagccttcta atgtttcaat
      481 cccatgtctc gtgtaaaatc gttctataat atcttttgct gttccttttg ttaacgtcag
      541 cctattttct gccataaatt tcataattat cctcttttct gtttaactta ccttaattat
      601 ttttgcgaca acaacaattc ttttcgttgt ttcactatat gcatcttcgc acgttgataa
      661 agtcattatt ctatctttta ccgttacatt aacatctgaa ttaattacag atttacgttt
      721 tgtttcatct aaaaattgtt gataatcttg atcattttca aaatctgtac gtatgtaatt
      781 atctttagta gtagttttat atgcactaaa tacttgcaat tgatatttac catatttatt
      841 gtcaaattca attatcttgt gtttttcata aaacgattgc tttaaataat cttctaacac
      901 atcaaacatc gtattatcac cgacatggtg cccgtataaa atagtattat gatttaaatt
      961 cttcaattca tttctaaaat ccataaaaat actaccttta cgtcgatgtt ctcgctcaaa
     1021 atctaaattt aaataatcgt gatttgtctt accttgtagt actggataat ttaatgatgt
     1081 tcctgataat tttatccatc caacaatgtc tttatttatt ttttcaagtg attcaaattg
     1141 tggtctcaca tgtgcttgat gtttgctcat cagcatttga aatttttgtt gtaatttctc
     1201 ataatttgcg cgttcttgct tgtcttcaat atatgtttga acaattttgt aaccaaaaat
     1261 gataataatt acaaccaata aaatttgtac aatagttaaa aatcgcttca ttctcataaa
     1321 aatcctcttt tattaacgac gtttcttcag tcatcactaa accagttgtt gtaccgtttt
     1381 agattcgatt tcgttgactt tgacaaatta agtaaattag cattggacca ccgacaatca
     1441 ttaaaatagc attggctgga atttctagag gaggctgtat cactcgtcct aataaatcag
     1501 ccactaacaa tagccataca ccaataactg tagaaaacgg aataagtact ctgtaattgc
     1561 ccccaactag ctttctaacc acatgtggca caataatacc taaaaaggct agttgtccaa
     1621 caatcgcaac agttgcactt gctaaaaata ctgctaataa acctgttaac catctgtaac
     1681 gatcaatatt aaaaccgata cttcgcgctt gtatgtcgtc taaatttagt aaattcaatt
     1741 taggggacaa tagtaatgtt aatattaatc ccaataatgc tgatactgct aatatgtata
     1801 cgtcgctcca tattttcatt gttaagcctt gaggaatttt cattaaaggg ttttgagtta
     1861 aaatttctaa aacaccattt aataatacga ataacgcaac acctactaat atcatactta
     1921 cagcattgaa tctaaattta gaatgcaaca atataattat taaaaatggt attaaacctc
     1981 caataaaact taataatggt aagtaaaagt acaattgtgg aataaacaac atacaaagtg
     2041 ctctcattat aagtgcacct gaggaaacgc caatgatatt cgcctctgcc aaaggatttt
     2101 gtagtgctgc ttgtaataat gctccagaaa ctgctaacat tgcgccaacc atcaatgcaa
     2161 ttaatatacg tggcaatcgc aaatcaatga ttgaatccac tgcttcattg ttaccagttg
     2221 taaattttgt aattaggtca ttaaatgaca atttaattgt accggttaca aacgaaatat
     2281 aagcagttgc gattaaaatg actaacaaac ataaaaatag tagtttcttt ttatttttta
     2341 tcatcatatc aactcactat tttttatcct tataaaataa atcatataat tgagtcattg
     2401 ccttatcagc atcaacattt gctgtaatac cgaatggcac ttcctctaag tcataaacat
     2461 gattattttt cacagcttta aaatgtttcc aaatatcatt ctgtttaaat tctttttgaa
     2521 acattttctt aacttcttca ggcattccgt gtggtaatct taaaataata tctggattga
     2581 tattcaacaa attttcagta ttagacgaaa tatattgacg atctttcact ttaataacat
     2641 tttctccacc tgctatttta actaaatcac caatatatga tttatcagtt gctactaaat
     2701 agctacccgg tacacccatt aatattaata ctttgggatg tttcttttgt ttagctgctt
     2761 tattttcaat tttttgcttt acagaattta aatggtcatt taattctttt gcttgtgctt
     2821 tacgattaaa ttgatcacct aattgtgtaa tcgacttttg catccctttt aaactatcaa
     2881 aatcataaaa gtagcctttc atatttaatt gtttgtaaaa tggttgcatt tcatctttaa
     2941 tcgttgacac actcaaaaca tgtgttggtt ttaatttttt aacagcttca acattcggct
     3001 ccattggttg accaatttcc ggtacatctt tataacgatt aggcaatgtc ttatatgacg
     3061 tgggtttgcc gacaattggt aaatccaatt tgtccaatgt cattgtcaat gcaacagttg
     3121 ttggtacgat tctgaattcg ccggatttag ttgattcttg agaactggaa gattgacagc
     3181 tggttaagat aacgacgctt atcactaaaa tggttaaata ctttatgatt ctcaaacttg
     3241 gttacctctc tttctgctgt ataaatgtgc cgaaataagt aaaatagttg ctaacacaat
     3301 gccagctgca aataaaatat attgcttttt atgatttggt tcatcaagtt ctttaatatc
     3361 actagataat tgactactat tcttgctatc atcatctcta ttaaaagctg gtaacgcttt
     3421 ttctttttca actacttttt tcttttcctt ttttgatttt tcatctttat gtttcggttg
     3481 ctctttgtga ttattttcaa ccttttttac tgttggaggg ctattttcaa cttttaatgt
     3541 acctgatgct ttaggtcgct cattttcaat ctttttatta ctgtcttgtt gcactttatc
     3601 tttttgtaca ggaatatcat ttaatccttt ttcagtaaaa aggctagtac gtctttcctt
     3661 agtatctgat ttttgactat cttttttgtc atcaatatct tttttaagct gatcttgatt
     3721 attttcttga ttttgatgtt ttgaaggcac ttcttttgca tcatcatgtt gtactttttt
     3781 ctctttaaaa tgaatagaat acaccatttc tttgttatct agatttttct tttcagccaa
     3841 cgttttaaca tgcatattga ttttatccat atgctgttca attaaaattc ttactattgt
     3901 gtcatccttt tcatctttta taacaaaagg aaattcttca tttttgtcat tttgaaaaac
     3961 aagttgttct ttatctattt gaccttttag ctttacttgc aagtaaattt taccagtcat
     4021 atctgaaatc aattgcgcct tttcgtcaac tagttgttgt tcctcagtta catttttatt
     4081 ttcttgatta aaaattagta actctaacgc tttattctct tttatctttt catcaaaaat
     4141 tgatgtagtt aattctgttt tatcttttgc ttcaggttga ttaaatttgg ctgatggata
     4201 ttttatctca tcagccaaca cgctaccaat cattgttgta tatgtaagta tacctaagct
     4261 aataatagct ataatagatt ttgtagcaat ttgtttaaca tttctcatta ttccacattg
     4321 cctttagatt tttttctaac aaacaatgtg attgcgatta ataatgctaa ggatgcaact
     4381 ggtattgcat atatatatgc aggtgtacca gcatttgttt gtggattttc tactttgtct
     4441 ttattcgaac tattagattc actttgacca gtagtcgttc catcagatcc tttgtcacta
     4501 cctgaagcag aatttttatc atctttacct ggtgcattag cacctgctac atcagttggt
     4561 ccattaaatt tatatgtaat gttgtaatga tggtcatatt tgaatggctt tccatttact
     4621 ttttcatcga tataaacgtc aatttttcca tctattttac cgttcaactt acttacttca
     4681 aattcagaag tgcgttcatc tttggcagtg tttttactaa taatattttc tttatgtcct
     4741 tcgatactca ttccagtaat ccaatgactg tggttgacag ttatttgaac atacaattta
     4801 ccatttttct taatgtactt tgccggttta ttaaaatagt cattagcaat tgacgtgtca
     4861 ttggtattgt atttgtaaac ctcataattc aaagtaccgc tatctgcggc atttgcagaa
     4921 ttactgaatg tcgcgatgat gataattaac gctaaaatcg ttgtattaaa aacttttaaa
     4981 atatttttca aaacataatc ctccttttta tgattgcttt taagtcttta gtaaaatcat
     5041 aaataataat gattatcatt gtcaatattt attttataat caatttatta ttgttatacg
     5101 aaaatagatg tgctagtata attgataacc attatcaatt gcaatggtta atcatctcat
     5161 ataacaacac ataatttgta tccttaggag gaaaacaaca tgacaaaaca ttatttaaac
     5221 agtaagtatc aatcagaaca acgttcatca gctatgaaaa agattacaat gggtacagca
     5281 tctatcattt taggttccct tgtatacata ggcgcagaca gccaacaagt caatgcggca
     5341 acagaagcta cgaacgcaac taataatcaa agcacacaag tttctcaagc aacatcacaa
     5401 ccaattaatt tccaagtgca aaaagatggc tcttcagaga agtcacacat ggatgactat
     5461 atgcaacacc ctggtaaagt aattaaacaa aataataaat attatttcca aaccgtgtta
     5521 aacaatgcat cattctggaa agaatacaaa ttttacaatg caaacaatca agaattagca
     5581 acaactgttg ttaacgataa taaaaaagcg gatactagaa caatcaatgt tgcagttgaa
     5641 cctggatata agagcttaac tactaaagta catattgtcg tgccacaaat taattacaat
     5701 catagatata ctacgcattt ggaatttgaa aaagcaattc ctacattagc tgacgcagca
     5761 aaaccaaaca atgttaaacc ggttcaacca aaaccagctc aacctaaaac acctactgag
     5821 caaactaaac cagttcaacc taaagttgaa aaagttaaac ctactgtaac tacaacaagc
     5881 aaagttgaag acaatcactc tactaaagtt gtaagtactg acacaacaaa agatcaaact
     5941 aaaacacaaa ctgctcatac agttaaaaca gcacaaactg ctcaagaaca aaataaagtt
     6001 caaacacctg ttaaagatgt tgcaacagcg aaatctgaaa gcaacaatca agctgtaagt
     6061 gataataaat cacaacaaac taacaaagtt acaaaacata acgaaacgcc taaacaagca
     6121 tctaaagcta aagaattacc aaaaactggt ttaacttcag ttgataactt tattagcaca
     6181 gttgccttcg caacacttgc ccttttaggt tcattatctt tattactttt caaaagaaaa
     6241 gaatctaaat aaatcatcgt cacactcata
//
LOCUS       DBNENW010000143         6261 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNENW010000143 DBNENW010000000
VERSION     DBNENW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.145"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            55..369
                     /locus_tag="AC1LW8_002224"
     CDS             55..369
                     /locus_tag="AC1LW8_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774028.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(375..512)
                     /locus_tag="AC1LW8_002225"
     CDS             complement(375..512)
                     /locus_tag="AC1LW8_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIP4774029.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(699..2096)
                     /gene="sufB"
                     /locus_tag="AC1LW8_002226"
     CDS             complement(699..2096)
                     /gene="sufB"
                     /locus_tag="AC1LW8_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIP4774030.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(2247..2711)
                     /gene="sufU"
                     /locus_tag="AC1LW8_002227"
     CDS             complement(2247..2711)
                     /gene="sufU"
                     /locus_tag="AC1LW8_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIP4774031.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2701..3951)
                     /locus_tag="AC1LW8_002228"
     CDS             complement(2701..3951)
                     /locus_tag="AC1LW8_002228"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIP4774032.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(4057..5364)
                     /gene="sufD"
                     /locus_tag="AC1LW8_002229"
     CDS             complement(4057..5364)
                     /gene="sufD"
                     /locus_tag="AC1LW8_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIP4774033.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVIGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(5462..6223)
                     /gene="sufC"
                     /locus_tag="AC1LW8_002230"
     CDS             complement(5462..6223)
                     /gene="sufC"
                     /locus_tag="AC1LW8_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIP4774034.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1907 a         1218 c          891 g         2245 t
ORIGIN      
        1 aaataacttt agacaagtac ttgtttatat ccaaatatag aaagaaggat taacatgtat
       61 tttgttttag caatatttac aataatcagt gccagtgtaa gtttaggtta ttcaattcaa
      121 gcatgtgcat ctagccataa tataaatgca tattatgcac ttagtcgaag cttaccttta
      181 tttttattag ctattttttc tttagtcatt catagtgcta tatttttgat aactatatcc
      241 attgcaatga tcttagttca atttttagat gcgattgttg gttataaaag tgaagatgtc
      301 tttaaaactt atggtccatt agcaacatct gtagtgaact taatattatt aatagttttc
      361 ttattttaac tcacttatac aacgaatctt aatcgaacta atatcgaatt atctaaagaa
      421 tatttgatgt agctttcaat taatttaata aagaccagca ctctaatgcc acaatcatgt
      481 tgtatttgtg ttgtcgcttt atccaccatc aatgattatt ttttacacca atcaaaaaat
      541 cgaactgata taaataagta caaagcttat ctatcaatcc gatttagtta taaaacaaaa
      601 aaagccacag taatgtggct ttttgttata ttcagtatca aaatggtatc aatagccatt
      661 ttcggaagtc aagaatggct taacaacgcg gtttaaagct atccaatact accttccatt
      721 tcgaacttga ttaaacggtt catttcgacc gcgtattcca ttggaagttc ttttgtaaat
      781 ggttcgatga atcccataac aatcatttct gtcgcttctt cttcagaaat accacgactc
      841 attagataga ataattgttc ttcagaaact tttgaaacct tggcttcatg ttctaatgat
      901 atttgatcgt tgaatacttc gttatatgga attgtatctg atgttgattc gttatctaag
      961 attaatgtat cacattcaat atttgaacga gcaccttttg ctttacgtcc aaaatgaaca
     1021 ataccgcgat aaataacttt accaccattt ttagaaatag atttagaaac aattgtagaa
     1081 gatgtattag gtgctttatg aatcatttta gcaccggcat cttgaacttg tcctttacca
     1141 gcaaatgcaa tagataatgt actacctttt gcaccttcac ctaaaagaac acagtttgga
     1201 tatttcatcg ttaacttaga acctaagtta ccatctaccc attccatatt tccgttttca
     1261 taaacaaaag tacgttttgt aactaaattg tatacattgt tcgcccagtt ttgaatcgta
     1321 gtataacgaa cgtgcgcatc tttatgcaca atgatttcca caacagcaga gtgtaaagaa
     1381 ctagttgtat aaactggtgc agtacaacct tctacgtaat gtacagaagc accttcatca
     1441 gcaatgatta atgtacgttc aaattgaccc atgttctcag agttaatacg gaaataagct
     1501 tgtagtggcg tatctagttt gatattttta ggtacataaa tgaacgaacc acctgaccat
     1561 actgctgagt ttaacgccgc aaatttgtta tctgctgcag gtactacaga agcaaagtat
     1621 tttttgaata attcttcatt ttcttgtaaa gcactatctg tatctttaaa gataatacct
     1681 ttttcttcaa gttctttttc catattatgg taaacaactt cagattcata ttgagcagaa
     1741 acaccagcta aatatttttg ttcagcttca ggaattccta atttatcgaa agttctttta
     1801 atttcttctg gcacttcatc ccatgaacgt tcagcttgtt ctgaaggctt tacatagtaa
     1861 gtaatgtcat cgaaattcaa ttctgataag tcgccacccc attgaggcat tggcatttta
     1921 taaaacaatt ttaatgattt aagacggaaa tctaacatcc attccggctc atttttcatg
     1981 ttagaaattt ctctaacgat attctcagtt aaaccacgtt ctgatctgaa aatggacaca
     2041 tcatcgtcgt ggaatccata tttataatcc ccaacatcag gtgctttttt agccatttca
     2101 atcactcctt aaaaatatat ttacaacggc gtgcgtaagt ttttaattca tgatgtaaac
     2161 catattataa caatgacatg acatcttata aaaattttta tacttttata tgtctaatat
     2221 caaaattatc tatgattaac agcattctat tcttcttcag tcgtaccttc tgctttacct
     2281 tctttagcaa cagtaccttt ttccaatgct ttccaagcta atgtggcaca tttaatacga
     2341 gctgggaatt gagatacacc ttgcaatgct tcaatatctc ccatttcttc tgtaatcaca
     2401 tagtcttcac caagcatcat tttcgtaaat tcttggctca tttgcattgc ttctccaagt
     2461 gaatgacctt taacagcttg tgtcatcatc gatgcacttg ccattgaaat cgaacaacct
     2521 tcaccttcaa acttagcatc ttttataatg ccgtcttcta tatcaaatgt tagtcgtata
     2581 cggtcaccgc atgtcgggtt attcatatct actgtcatag acccgttatc taatacacct
     2641 ttatttctag gatttttata atgatccata atgacagatc tatataattg atctagatta
     2701 ttaaaattca taagagaaaa actccttcgt ttgtttcaag gcatttatta actgatcaac
     2761 gtcttctttc gtgttgtata tataaaaact cgctctagct gttgaagaca catttaacca
     2821 tttcattaac ggttgcgcac aatgatgccc agctctaacc gctacacctt ctgtatctac
     2881 ggctgtagca acatcgtgtg gatgtacatc ttgtaaatta aacgttatta cacctgcacg
     2941 acgatccttt ggcgggccat aaatttcaat tccttcaatt gcagacattt gctcataagc
     3001 atatatcgtt aattcttgtt catatttatg aattgcatca aaacctatgc gttctaaata
     3061 gcgaatagct tctgcaagcc caattgcttg agcaattaat ggagtacccg cctcaaattt
     3121 agtaggtaaa tcagcccatg ttgcatcata cttacttaca aaatcaatca tgtcgccacc
     3181 gaactcaatc ggttccattt tttgtagtaa ctcacgttta ccaaataata cgccaatacc
     3241 tgttggtcca agcattttat gaccactaaa actataaaaa tcagcattca tttcttgcat
     3301 atcaagtttc atatgtggtg ctgcttgcgc cccatcaaca ctgataattg caccatgttg
     3361 atgagctatt tctgcaatgg ttttaacatc attaattgta ccgagcacat tagatatatg
     3421 tgcaatagca acgatctttg ttttatcatt aatcgtttgc ttaatatcct cgatgtttaa
     3481 ttcaccgtca gctgtcattg gtataaattt caatgtcgca tttttacgct ttgctaactg
     3541 ttgccaagga acaatattgg catgatgttc catttcagtg acaacaattt catcgccctc
     3601 ttcaacattt gcatcaccat agctatgtgc tacaaggtta atcgacgcag ttgttccgcg
     3661 tgtaaaaatg atttcttcaa aatacttcgc attaataaaa cgacgaacgg tttcacgggc
     3721 attttcataa ccatcagttg ccaatgatcc taatgtatga acaccacgat gaacgtttga
     3781 attataacgc ttgtagtaat cttctaaaac atttaacact tgcacaggcg tttgacttgt
     3841 cgctgttgaa tcaagatatg ctaaacgttt gccattgact ttttgatcta atatcggaaa
     3901 atccttgatt acttcattaa cgtcaaatga gtgttcggcc acttcattca cgacctttct
     3961 taaataaaaa tcctaatcat ttaaatactg acgttgtatt agtcttatac caatatcgac
     4021 agtctatatc tattacaaac ttttattttc aaaatattat ttagaaactt tgcgttcaat
     4081 tacttctctc aattgacgtt taacgtcttc gataggtaat tcacgtacta ctggatctaa
     4141 gaaaccatgt ataacaagac gttccgcttc tctttgagaa ataccacgac tcattaaata
     4201 gtaaagttga tctggatcaa cacgacctac tgatgcagca tgaccagctt gtacatcatc
     4261 ttcatcaatt aataaaatag gattcgcgtc accacgagca tgttcagata acattaatac
     4321 acgtgattcc tgattagcaa ttgatttagt tccaccatgc ttaatgtagc cgataccatt
     4381 aaatacagac gatgcatgtt ctttcataac accatgttta aggatataac catctgtttc
     4441 tttaccatat tgtacgattt tagatgttag attaattttt tgttcgcctg tacctataac
     4501 tactgattta agtgaacttg ttgaacgatc accaaataaa tttgttgtat tatcaataat
     4561 ttggctaccc tcattcatta aacctagtgc ccaattaatt gaggcatccg cttcagtaat
     4621 accacgtcga atgatatgac ctgtaaagcc tttatccata tagtccactg agccatatgt
     4681 gatatttgaa tttgcaccag caatcacttc agaaataata tttaattgat ttccttcacc
     4741 agatgcattt gataagtaat tttcaacata tgtgacttcg gcgctttctt cagtaacgat
     4801 gataacatgg ttataaaagc ttgcattttc gtcgtcgtgc aacacaacgt attgtactgg
     4861 atgttctaca actacatttt taggaacata aacaaatacg ccaccattaa ctaatgccgt
     4921 gtgtagcgct gtgatacgat gttcatctac tgttactgca tctttcataa agtacttttg
     4981 tactaaatca ctatggttca taagagcgtc tgctaaacct tcaacgataa cgccatcttt
     5041 cgatgcatta tcatcaactt gtgtgtacgc aatcgtatta ttatgttgaa ttactaagtt
     5101 tttagaatga tctacgtcaa taatttctct tactgactca ggtaattgtg ataaagattg
     5161 ataaacatca ccttttacat cgtgttgttt aaaagaatca aaatcccatt ttcttaattt
     5221 tgttttatca ggttttggca tttctaaagt ttctgttaat ttcaaagctt ttttacgtaa
     5281 ttctgtcatc caagaaggtt cattgtgggc tttagaataa tcaacaagtt gttcttcaga
     5341 aatgttcaaa atatcagttg tcataacttt ttcctcctac ttgatgaaat ttactatttc
     5401 gtataaatgt tcattttcat catatataga tgaacatctc atggatactg tattaataag
     5461 attattcagc tgaaccgaac tcttctttaa cccattcata tccttcttct tcaagacgtt
     5521 ttgctaattc tggaccacca gatttaacga ctttaccagc atacattaca tgtactttat
     5581 caggagtaat gtaatttaat aatcgttgat agtgtgtaat cattaatgca ccaaagtttt
     5641 ccccacgcat ttggttaata cctttagata caacttttaa tgcatcgatg tctaaccctg
     5701 aatcgatttc atctaagatt gcaaacttag gttctaacat cattaattgt aagatttcgt
     5761 tacgtttctt ctctccacct gagaaacctt catttaaata acgttgtgcc atgtctttat
     5821 ctatgtctag aaaatccatg tttttatcta atttcttaat aaattgcatt aagttgattt
     5881 cttgtccttc ttcacgtttc gcattgattg ctgaacgcat gaaatcagca tttgtaacac
     5941 ctgtaatttc tgatggatat tgcattgcca agaataatcc tgcttttgct ctttcatcaa
     6001 cttctaattc taaaatattt acaccgtcta aaagtacttc tcctttagtt acttcatagc
     6061 ttgggtgtcc cataattgca gatgataaag ttgatttacc tgtcccgttt ggtcccataa
     6121 tcgcatgtat ttcatcagtg ttaattgtca agttaacacc ttttaagatt tctttatcct
     6181 caatagacac atgtaggtct ttgatttcta atgttgatgc cattttatat tccctccgta
     6241 aaatataaag ttttcttaac t
//
LOCUS       DBNENW010000144         6163 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNENW010000144 DBNENW010000000
VERSION     DBNENW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.146"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(248..1126)
                     /locus_tag="AC1LW8_002231"
     CDS             complement(248..1126)
                     /locus_tag="AC1LW8_002231"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIP4774035.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSESLFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1146..4586)
                     /locus_tag="AC1LW8_002232"
     CDS             complement(1146..4586)
                     /locus_tag="AC1LW8_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIP4774036.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTMINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLKQMDRSYVEMITEFETNKADISRRQQRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4890..>6163
                     /locus_tag="AC1LW8_002233"
     CDS             4890..>6163
                     /locus_tag="AC1LW8_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HIP4774037.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFG"
BASE COUNT         1929 a          988 c          917 g         2329 t
ORIGIN      
        1 taatcaaata tgttttttag attaataaag ttgtaactgt actattttgt agtgtatggt
       61 aatttatttg gatgaaatat aattctgtaa actctattga ttaatcaaat atgtatctat
      121 caaaattcgg cttatttatt cactctgaca tatccaaata cgcaaaaaga ctatttctac
      181 cttgtatcga tagaaatagt ctttttatac ttaattggtt taatttttca taaaatttta
      241 tactttttta aaaatgggat gaaatatatt ttcctgaaac atgtaattca tgttcgttac
      301 aaattgataa aatatgatta agtgttgttg caaatgacat tttctcaaaa agcgattcac
      361 tatcaattgg tacttgtgtg tgaatttcag caagtcgctt cgataaatat agttcatcta
      421 aattgtcatt aatcttatta cgttgcccag ctgataatgc atcaatgttt tcaaccacat
      481 tttcaacgct ttgatattgc tgaattaact taatagccgt tttctcacca attcctttaa
      541 cacctgcata tccatctgct gtatcaccca taaacgcttt aatatcgatt agttgttgtg
      601 gttcaagggc atattcttcg ttaaaacgat gtaatgtata tctattataa atgttaaaac
      661 cttttttaat tagccagact tcaacattgt cattaataca ttgcagtaaa tctttgtcgc
      721 ccgtaataat atagacatcg ttatcagttg aatattgttg tgctaatgta cctataacat
      781 catccgcttc atagttttta acgccaatat ttacaaagcc aaattgctct gaaatttctt
      841 taacataatc aaattgtggt atcaattctt ctggtggtgc agaacgattt tgcttataac
      901 catcaaacat atcatttcta aaagttgatt gtcccatatc ccaacataca gctacatgtg
      961 tagggcgtat ttcatgtatt gccgaaaaga tatgacgcac aaacccttgt attccatttg
     1021 taggtacacc ttgtgaattg tacataaatt gtttatgaag acttgtagcg tagaaatgtc
     1081 taaataatag tgccatacca tctacaagta atattttatt aggcatttaa aacgttcctt
     1141 ctttcttaat ataaaatagt tttcagttgt tgatgtttat ctttcaattg attgataagt
     1201 gttgtatcta aatcaaatgc taataaatcg tttatttgcg tttgaatatt agcttcaaaa
     1261 ctttcaaatt gcgattcagc ttctacagcc atttgcttaa catataattc aagttgttgc
     1321 ctcaataatc ccatttgtgg ttgtaataat cctaatgtac tttgacaaat tgattcatgt
     1381 atatctcttt gcccatttgg attcaaaatt ttacgttttg ttaattgttt aggcaatgca
     1441 tttagcatat cattgaaatc gatgtgcaat aatggttgct ctatattagc tgattcaaag
     1501 ttaaactgag gattaacaat gacatgtaaa tctgctaatt gttgaacgat tggtgcaatt
     1561 tgctcagtga gttgcttatt aaagtatttt tttatacgtt ctgtaattaa agattgctct
     1621 aaaaacaatc gttgatgaat ttgatctaag tatactttcg tagacacttt cttttcttca
     1681 ttaaaatcac tattttgcgt catttgagaa ttaaacactg atttaacatc gtcaagtagt
     1741 tgtaatttta aacgtgcatt taaatgataa acttgttctt caacttcgtt gtctgtatgt
     1801 tgtaacgtcg catcaattaa ttgatgttgt aaacgctgtt tatctttata gactgttaac
     1861 ctttgttggc gacgtgaaat atcagcttta tttgtttcaa attctgtaat catctctaca
     1921 taagaacgat ccatttgttt aagctgatga atcatttgtt gttctaaaat tgatttagat
     1981 tcaacatcaa caaattgttg tatgctttgt tttaattgat caatgccctt atcttcagct
     2041 tgtaatgcat ttcgacttga tacagcaaaa atgtctgatt gtaagtgtac ttgtcttaat
     2101 gcatctgata catatgtttc aaccgcttca agatcatctt gactttctgc taaatcagca
     2161 gcattaatta ccattttaaa tgcttggttt tcattcaact ggttcatatc tttcatgtgt
     2221 tctataaacg ccttgtcatt atcagtaaat gaatgattaa aataacttac atacaatatt
     2281 aagtctgaag aagttaaaat ttgctcggtt tcatttgtat gcctttggtt atttgagtgt
     2341 agccctaatg aatcaacaat tattttacct tttaaccaat catgcattaa tgcaatgtgt
     2401 actgtcttaa caaatgttgc atattcatct tctgcactcc actttttcaa ttcctgttga
     2461 ttaatggcat gtttttctcc attttccaac atattgacat acaatttata atgtttttca
     2521 actgcctgaa taaaagcgag ttgattttta tttaaatgtg attttaactt ttctaaatct
     2581 gaattaataa agtcttctat agtcgaataa gacatatctt ggtattcaac tactgcatta
     2641 atttcatcta ataattgcga ttgtgatttt agcgttatat aactctcgtc tccataagat
     2701 atttctgtag tagctgctgt agtaggattt ggagaactga ctaaaatatg ctcgcctaat
     2761 aatgcattta tcaaactact tttaccagca ctaaatgttc caaatacacc tatttttatt
     2821 aatttattat ctatacgtgt taatgtttcg tggatatctt gctttgtacg cttgaacaaa
     2881 ggcacatccg aaattatatc aagcgctttt tgaatatcta tagacatatt tgttgtagct
     2941 gtattttgat ttagctgctg attatcacga ttatcctgag tattatcagt agccacttga
     3001 tatgttgtct cttgtcgacc tattaattta tctagagatt catctaaatg aatatagtag
     3061 tgacgataat tcttagtcgt caatgactga cgcagctcat ttaattctgt ataacgttga
     3121 tattctttta aatcatcact ttcttctgtt ggtaattcat ctgcctgtac attttctatt
     3181 atttctttaa aaattggtgt tgattgttgt tcaacatatt tcttaatggc tttaaccact
     3241 tcgtctgaaa atgtaagtac ataagtattg ctgattgatg tttgtggttg atataaatct
     3301 tcaatcattt ccggcttaac gtcataatgc tgatttaata ctttatctga agcttctttt
     3361 ttattgataa aacgcgttac aaatgacata tcttctcgca ttggttgacg aatttgttga
     3421 ttaacatgtt cttgtaacgc atctgtcgct gttaataatc gctgttgttg aatttgtagc
     3481 tttttcttct ttttattaaa aaacccacca acattaaagt cttgagacat actttccaaa
     3541 taacttcgta acatttctct catattatgc ggcataatgt atgcattttc taaaatattt
     3601 ttacgcttat tctttaaaaa tgccattaat tcatctggat tatttaaaag ttgtgcctct
     3661 tcactaattg cttgatgttg ttgactattt aaaaatgctt gttcaaactc cgcttcttcg
     3721 atacctaaat cttctagtac ttcctgaatt tcagactgaa tgtaatctag ctgagcttcg
     3781 gtaatgtatt caaccgttct tgatgtataa tcctctattg tctctctatg ttgatctaat
     3841 gaaattagat aacttgataa tacttcaagt tcattttcag ggtgatcaaa tttagataca
     3901 taaaaggtgc gttctaattt aataccccaa tctgcaattg atttttcaac tcgagattta
     3961 aacgtagaga atgacaattc atcgtcttga tgcttgtcaa tttgattaat gataaacaca
     4021 acaggtattc caacatcatt tatatgcttc ataaacttaa agttaagttc agattgaacg
     4081 tggttatagt caaccgtata aaatatcata ttacttgtat acatatattg ttctgttatt
     4141 gactgatgtg atgcaacatt tgaatcaaca cctggtgtat cttgcaacgt aaacccattt
     4201 tcaaatttag ctgattgaaa attaatttct acagattcaa cgtcgacatt ttggcgattc
     4261 atttccctta cttcatcata attagataat ttggcatacg tttgattcgg caaattagca
     4321 ataatatcgt gattgtctga aactgacaca atagcagtat tacttgtcgt tggtacagga
     4381 gaacttggta agatatcttg ttcaattaat aaatttatca ttgtcgattt acctgcagaa
     4441 aaatgtccaa cgaacgaaca tgtatattgc tgcaaatata ctttcttaat tacttggtta
     4501 attgtatgta aaagtgcttc atttcgcgac ttttcaactt ctttttttaa tttatataaa
     4561 agatctaatt gttctttatt aatcatgtta ctttcccctt tggttcgttc acatagaaat
     4621 gcttgcttgt tacaacgaat tcatacaata tcgatgtcca tttaaacata gatcgttaaa
     4681 tggctcgcat tgtattatgt ttaaatttta tcatagctgc ttgataaaac atacgaataa
     4741 tcaattttct tcaaacattc caacttaaaa gttcattttt ttaaaaatca caaatcatcg
     4801 ttaaattttt ctagaaaata aatattgtca cagtttgttc acacacgtca cagaattgac
     4861 accgtataat agccaattgg gagggaaata tgaaaaatac atttaatcca ttacaaagat
     4921 tacatttcta tgcagcaata tttattgctc cactgttaat cactttaacc atttcgggca
     4981 ttggttactt gttctttcca gaagttgaaa ataatattta taagaatgag ttttttggtg
     5041 acagtgatgt aaaaacgcat caaacattaa atgatgcagt acatcaagtt gaacaacaat
     5101 atgaaggatt ctttgtaagt aaagttagca tacttgatga accatataac aaacgaatta
     5161 cactgagtga tatggcagga aatcaacgtt acgtctttct agatcataac aatcaatttg
     5221 ttgcagatca aaatgcgaaa catacgtatt ctaatgtgat gcgaagtata catagttctt
     5281 tgtttactga aaatactatt attaattatt tagtagagtt aaccgcatgt tggatgatat
     5341 tcatgatttt atctggtact tatttactca ttaagaagca tttaatttct aacaaaagta
     5401 aggcacttcg ttggcaaaag tggcacgcaa tgattggagt tatcattgca attccagtat
     5461 ttgtattagt cttaactgga ttgccatggt ctggttttat gggcagtaaa attgccggta
     5521 tgatggacac aaacggtgac cttggtcaag gtgaattagc gattaatcca cctaaatcag
     5581 atttgaacga attaccttgg gctacacgta aaaataaaca gccagcttca tccgaaaaag
     5641 gttcaagtgg tcatcatggt aatgcagcaa tgcctcaaac caaattagat tatcaaatat
     5701 ctattgataa ggtcgttgaa caggcgcaaa aagctggtat taaaaagccg ttttcaatcg
     5761 tatatccaag tgataaaaat ggtaccttta ttgtatctaa tactagtaat tcaggtgtta
     5821 ctgggctaga tgtatcacca tacaaggaac aaacacttta tttcgatcaa tatagcggta
     5881 aaaagctagg tacgattaaa tatgatgact acggtattat tgctaaatgg tttacatggg
     5941 gcattccgct tcacgaaggt catttattcg gcattttaaa taaaatcatt aatttatttg
     6001 tatgtatcgc tttattagta gccattggca tggggtttgt ctcttggata aagcgtacaa
     6061 aaaatactgc agtaaaagta ccacatcgcg taaaaaaacc agcatctata tcactcataa
     6121 tatgtttaat tgtattagga ttattaatgc cattatttgg att
//
LOCUS       DBNENW010000145         6070 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNENW010000145 DBNENW010000000
VERSION     DBNENW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.147"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(102..1475)
                     /locus_tag="AC1LW8_002234"
     CDS             complement(102..1475)
                     /locus_tag="AC1LW8_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIP4774038.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEKFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(2147..2377)
                     /locus_tag="AC1LW8_002235"
     CDS             complement(2147..2377)
                     /locus_tag="AC1LW8_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774039.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(2408..3346)
                     /locus_tag="AC1LW8_002236"
     CDS             complement(2408..3346)
                     /locus_tag="AC1LW8_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIP4774040.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(3782..4447)
                     /locus_tag="AC1LW8_002237"
     CDS             complement(3782..4447)
                     /locus_tag="AC1LW8_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP4774041.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            4599..4919
                     /gene="czrA"
                     /locus_tag="AC1LW8_002238"
     CDS             4599..4919
                     /gene="czrA"
                     /locus_tag="AC1LW8_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HIP4774042.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4921..5901
                     /gene="czrB"
                     /locus_tag="AC1LW8_002239"
     CDS             4921..5901
                     /gene="czrB"
                     /locus_tag="AC1LW8_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HIP4774043.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHAH"
BASE COUNT         1929 a         1131 c          834 g         2176 t
ORIGIN      
        1 ggttgattac ccgtcatttt ttctttatgc ggttattcaa atatgcaatt atgcaatttt
       61 taatcctatc cttacttctt ttcaaaaact accatatatc attagtcatg tttgactttg
      121 actatacgtg tgtattttaa aaactgctct gaatatttct ccttaatttc ctctaaactg
      181 tcatatgtaa tacctacaat atgccaatta ggattgaaat aatagaaaga atctttttgt
      241 cgtgaccatt taatcatatt accgtcttta ttataacgtg gtaatgttac ttcatagcct
      301 tctaacacat taatatacgt ttggaaaatg tctgtatcta tacgattaaa attatcaata
      361 actaaataac ttttatcctt tttaggcatt aattgagtaa taaacccttc acgatagtat
      421 aatgcccctg tttcatttgg taaatacttg ccataaagca tatcttctga aaaatcagga
      481 tgacctgttg ttataacagg atttgcattc gctttttgca ataagtcatt agccgctttt
      541 aagccttctt cttttcgatc tacaacaagt aaaataaatg gctttaacgc ttcttctttt
      601 tcactttcaa gcatatctgg tttttgaacc atttcaaatg gagatttcaa tccattatta
      661 tcgctcattt caataattgc atcatactgt gcttgtgaca tacttgcaat agcctgtttt
      721 gcattttctt gaaggaaata taagtttttc aatttaggat gcttatttaa tgtacttaat
      781 gtaatcggtg taatgtcttt ctcataagac acttcaatca ctgtactatt tgttctacca
      841 ggaattggtg gtttttcatg aatatgcttt gatacttctc caattccaac gacagattga
      901 tttttcgttc gattataaaa aataatattg tcgccttctt ctaactgagt ataaaaatga
      961 taaccattac gtttaatacc gttgtacgtg tgcgtataaa tcgtatattg gtttccaggt
     1021 tcaaattttt cagtttcagc taaaaagaaa taacgcggta tcttaatttc gcctttacca
     1081 agaccactta ttaaatcaaa ctcttctgca gtgatttgat tgaacaatgt ctctttcata
     1141 ttactcatac gaaattccaa agcttcacta cgctttaaat aatctgctgt taatggtttc
     1201 aattgttcat taaaacgaaa ctgtacacgt attttatttt gtgcacctgt ttcaacacta
     1261 ataatttcac cacatccaag tagtccagta tccgtctgaa cttgataaaa gatgacttga
     1321 tctcctactt tagccttttt aaacgctcta aatccttgag atgggttaaa atgtgcgcct
     1381 gattcaaata aagctgtttg tcctactaac ggttcattat gattccaacg gttatatcca
     1441 caattcaacc aaaaataatt cgtttctgct gtcatcttaa tactccttaa cctgaataaa
     1501 ttttagaaac actatgaatt acattctttt agtgtttctt atgcagttgg acgcgtatgc
     1561 gaacaactgt ataccctttg ttcactgcga ttttaatcgc atttcctata acattgtagc
     1621 gcccaggaca ttaatttacg tcccagaccc ttatcgtttt cacttctaag taagtcgaac
     1681 tattttgctt tacaacaagt gcgactctaa atacagttgg acacacatac gagcaactgt
     1741 atacctttta atcagttttc tatattttat ttattatatc tgtcttaatg ataaaaattg
     1801 ttacaaacag tttaacatat ttagctacct attttgcata taaaaaacat gcttgcatac
     1861 actatgcaat aaaaattaca aatttatata tgataccact aagcttgctt atctctatag
     1921 cgccattgat acacattttt aaatatctat actgccgtta gaattttatc atgtcttaat
     1981 tttcattaaa tattaattac ttcattttat ataaaccaac aaaaaccccc tcactactat
     2041 gcaagtgaga ggttatgttg atgtgcttta ttttcatcaa tgtcattaac acgatcatat
     2101 ttcttctgtt aatgcactgt actttattca aacgcgctat cgacaattaa tttgaagcta
     2161 caaaggtacc gcataatggc agcacattaa ttaacaatgc gctaaacttt aatgaggcgc
     2221 tgatttgctt gtctttaaaa gtcaacatag cactgataat tcctaaaata gctactatac
     2281 gtggaattgt catgctcgga tactgcggta tatgtaagaa agcaccaaga cctgctatca
     2341 caccacatac taacgcaata tatagaaatt gcttcaaatc attcactcct aaattgttat
     2401 tacactatta cacatagcta atcatcaatg tgaaatcacc ttcaaagaca ctatccaaat
     2461 cttcagaagt caaaataaag tttgtaccag tagtcagttt gaaaatttca ccatcgacaa
     2521 tcatttgtcc ttcaccttcc aacactgtaa ctaaacagaa ctctctaggc ttcatataat
     2581 ttaacgtgcc agaaatttcc catttaacca atgtaaagaa atcattcgat acaatgtgtg
     2641 tacacttatg gttttcaata atttcacttt caggcaaaat attaggtaat ggtgcattgt
     2701 actgaataac gtctaaagct ttttcaatat ttaacggtct atcattatat tgattatctt
     2761 gacgattgaa atcataaagt ctatatgtaa tgtctgacga ttgcatcgtt tcgtatgcta
     2821 atattcctga agaaatagta tgtactgttc ctgctggaat aaaatagaat tctccaggtt
     2881 ttactttaat atatctaagt atcgactcta tcgttccgtg ttgaacatga ttcgcaactt
     2941 cttctctaga ctctgctaaa gtccctataa ctatttctgc atcttcttct gcatctataa
     3001 tataccaaca ttcagatttg ccatattgcc cgttttcatg ctcataagca taagaattat
     3061 cagggtgcac atgaatagaa agtgattctc ttgcatccac tattttagtt agaagcggaa
     3121 aatctttgct tgggaaatca ccaaacaatt cacgatgttc tgaccaaata cggtctaatg
     3181 tttgaccttg atatggtcca ttaataatct cgctcgtacc atttggatgt gctgacacac
     3241 accaacattc cccagttgta tcattgtcta attgatatcc aaactcactt agacgttgac
     3301 cgccccataa ttttgttttt aaaattggtt gtaaaaataa tggcattgtt gcacctccat
     3361 tgtgattaag taagcaatag aactctgatg ttgttgttcc attatatttt gattttgttc
     3421 tcatttacat cgtattataa acttccacat ttcaaattaa ctattagtga ttgtaccata
     3481 tttactaaca ttgcagtact gccaattaaa gcgcttcact taaatttaca gtactttaac
     3541 atttttaaaa attaatagca tagagattat ctctctctta catttgtaca catttccctt
     3601 taaatttact cgcccattat accaattaat aaacaacttt aatagttgtg ccatacattg
     3661 ttcaaattct ttgtaaaatg catagacaat acgtacttat tcatacttat aattcatcat
     3721 tttcaaaaaa ataacgagtt acgaaaacgt aacccgcttc aaatcatatt tactatcctt
     3781 attaatcctt ttcaatttca aattgagtta aagcatcttt aatgtcctgc tcaccactaa
     3841 taatttgaaa ctcttggtga ttaaaatgat tggatgtgac aatttctttt aatactgtcg
     3901 caacatcttc tctaggaatt tcacctttac catcaaaata ttgtgcagct tctatctttc
     3961 cagatcctgc tgcatttgta agtgcccctg gatgtaaaat tgtataattc aaacctgaac
     4021 gtcttaaata gtcatcagcg taatgtttag ctattgtata tggctttaaa tcaccgctat
     4081 catcaaaagc ctgacgtctc gaatcatatg ttgaaaccat gacatagtgt ttaatattag
     4141 cctctttact cgcaatcatt gatttaacag caccatctaa atcgacaata attgttttat
     4201 ctgcacccgt gttccctcca gaacctactg aaaagataac tttatcgaat ggtttaaacg
     4261 tctcagttaa agtctctatt gaatcatttt caacatcaac aagaattgct ttcatacctt
     4321 gtgattttaa cgcattaagt tgatctgatt gcctaacacc agcagtaaat ggtacatttt
     4381 cttttgctaa ttgttgcact agtaacgaac ctacaccgcc attagcacct ataaccaaaa
     4441 tattcattta caacactctc ctatttatta ttctctatgc cataccactt tatgagatat
     4501 gtaaaacttg ttacaactat aaaaatcaat tgacatacta ctgggaacgt attaaattaa
     4561 tatatgaaca aatattcata tgaaaggatt gtcatataat gtcagaacaa tattcagaaa
     4621 taaatacaga tacattagaa cgcgtaactg aaattttcaa ggcattaggc gattacaatc
     4681 gaatacgtat catggaattg ttatcagtca gtgaagcaag tgttggtcac atttcacatc
     4741 aattgaattt atctcaatca aatgtctcgc accaattaaa attacttaaa agtgtgcatc
     4801 ttgtgaaagc aaaacgacaa ggccaatcaa tgatttattc attagatgac atccacgtag
     4861 caactatgtt aaagcaagcc atacatcacg cgaatcatcc taaagaaagt gggttataat
     4921 atgtctcatt cacatcatca tcatgaccat atgcatagtc atgtaactac aaataataag
     4981 aaagtattgt ttatatcgtt tttaataatc ggtctatata tgtttatcga aatcatcggc
     5041 ggtctccttg ctaacagctt ggcattacta tctgacggta tccatatgtt tagcgacaca
     5101 ttctcattag gtgttgcact tgtcgcattt atttatgctg aaaagaatgc cacaactaca
     5161 aaaacatttg gttataaacg tttcgaagta ctcgcagcgt tatttaacgg tgtaacgctt
     5221 tttgtaataa gtattttgat tgtttttgaa gcgattaaac gtttctttgt tccttctgaa
     5281 gttcaatcaa aagaaatgtt aatcattagc attattggtt taattgtcaa tatcgttgtt
     5341 gcattcttta tgtttaaagg cggcgacact tcacacaatt taaatatgcg tggtgctttt
     5401 ctacatgtta tcggagactt attaggttca gttggcgcca ttactgcagc tattttaatt
     5461 tgggcatttg gatggacaat cgccgatcct atcgcaagta ttttagtttc cgttattatt
     5521 ttaaaaagtg cttggggtat cacaaaatct tctattaaca ttttaatgga aggcacacca
     5581 agtgatgttg atatagatga agttataact actattaaaa aggattcacg aatacaaagt
     5641 gtgcatgatt gccatgtttg gacaatttca aatgatatga atgcattgag ttgtcatgtt
     5701 gttgtagacc atacattgac aatgaaagaa tgtgaattat tattagaaaa cattgagcat
     5761 gatttattac atttaaatat tcaccatatg actattcaat tagaaacgcc taatcacaaa
     5821 catgatgaat cgattatatg ttcaggaaca catagtcatt cacataacca tcatgctcat
     5881 catcacgcgc atgcacatta ataattttaa cctactgcca ttgcatcgat taaacttttc
     5941 aatggcagta ggttttttat gtctttatgg cgacttgttt ggtctttgat gatgcaatgt
     6001 ttattaacaa attttcaact attatttctt acattagtca tatttttgac aatttactat
     6061 tataattctc
//
LOCUS       DBNENW010000146         6046 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.148, whole genome shotgun sequence.
ACCESSION   DBNENW010000146 DBNENW010000000
VERSION     DBNENW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.148"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(338..426)
                     /locus_tag="AC1LW8_002240"
     tRNA            complement(338..426)
                     /locus_tag="AC1LW8_002240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:Ser,seq:gga)
     gene            complement(481..552)
                     /locus_tag="AC1LW8_002241"
     tRNA            complement(481..552)
                     /locus_tag="AC1LW8_002241"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Glu,seq:ttc)
     gene            complement(554..628)
                     /locus_tag="AC1LW8_002242"
     tRNA            complement(554..628)
                     /locus_tag="AC1LW8_002242"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Asn,seq:gtt)
     gene            complement(643..716)
                     /locus_tag="AC1LW8_002243"
     tRNA            complement(643..716)
                     /locus_tag="AC1LW8_002243"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(682..684),aa:Gly,seq:tcc)
     gene            complement(732..807)
                     /locus_tag="AC1LW8_002244"
     tRNA            complement(732..807)
                     /locus_tag="AC1LW8_002244"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:His,seq:gtg)
     gene            complement(821..893)
                     /locus_tag="AC1LW8_002245"
     tRNA            complement(821..893)
                     /locus_tag="AC1LW8_002245"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(858..860),aa:Phe,seq:gaa)
     gene            complement(910..985)
                     /locus_tag="AC1LW8_002246"
     tRNA            complement(910..985)
                     /locus_tag="AC1LW8_002246"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Asp,seq:gtc)
     gene            complement(1007..1080)
                     /locus_tag="AC1LW8_002247"
     tRNA            complement(1007..1080)
                     /locus_tag="AC1LW8_002247"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1044..1046),aa:Met,seq:cat)
     gene            complement(1550..2104)
                     /locus_tag="AC1LW8_002248"
     CDS             complement(1550..2104)
                     /locus_tag="AC1LW8_002248"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RBBP9/YdeN family alpha/beta hydrolase"
                     /protein_id="HIP4774044.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2426..3826)
                     /gene="hemY"
                     /locus_tag="AC1LW8_002249"
     CDS             complement(2426..3826)
                     /gene="hemY"
                     /locus_tag="AC1LW8_002249"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIP4774045.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3850..4773)
                     /gene="hemH"
                     /locus_tag="AC1LW8_002250"
     CDS             complement(3850..4773)
                     /gene="hemH"
                     /locus_tag="AC1LW8_002250"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIP4774046.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4831..5868)
                     /gene="hemE"
                     /locus_tag="AC1LW8_002251"
     CDS             complement(4831..5868)
                     /gene="hemE"
                     /locus_tag="AC1LW8_002251"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIP4774047.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5858..5968)
                     /locus_tag="AC1LW8_002252"
     CDS             complement(5858..5968)
                     /locus_tag="AC1LW8_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774048.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
BASE COUNT         1879 a         1212 c          890 g         2065 t
ORIGIN      
        1 agctaaacat tacatttcaa aacgattagg tcatgcagat gtacatacaa cgcttaaaac
       61 atattcgcat ttgattaaag aatttgaaga taatgaaact aaatttatag aaaaaaatct
      121 gaatgatttg ttttgtgact aaacttcaaa atcttgtgac taaagttaag attaatcatc
      181 aaaaattagt cacattgtgg ctaaaaaatt ttaaatggat ataaataaat aagaagtgta
      241 aaaatgagga taatagctca aatgcagata tatcaagggt ttgagtctaa aataaaaata
      301 gcgcttaaaa tgggtaaaac caatttaagc gcctttacgg aaggtaaggg attcgaaccc
      361 ttgagacgct tgtgacgcct acacactttc caggcgtgct ccttcggcca gctcggacaa
      421 ccttcctcaa caataaatat gtaataaaaa aacagaagcg atatttcact tctgtttgta
      481 tgactcctac gggactcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt
      541 gaccaaggag ccatggctcc acaggtagga ctcgaaccta cgaccgatcg gttaacagcc
      601 gatagctcta ccactgagct actgtggaat aatagaaaat aatggagcgg gtgatgggaa
      661 tcgaacccac aacatcagct tggaaggctg aggttttgcc attaaactac acccgctaat
      721 gtaaacgatt atggggcggc tgaaggggat cgaaccctcg aatgtcggaa ccacaatccg
      781 atgtgttaac cacttcacca caaccgccat gaattaagaa tggttcagga cagagtcgaa
      841 ctgccgacac atggagcttc aatccattgc tctaccaact gagctactga accttaatgg
      901 taataataat ggcggtctcg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      961 gtgttaaccg ctacactacg agacctatta aattaaaaac tatgtattgc gggaggcgga
     1021 tttgaaccac cgaccttcgg gttatgagcc cgacgagcta ccgaactgct ccatcccgcg
     1081 ctaatattat tttgaattac ctaattaata tatcataatc aaaaacctaa agtcaagaac
     1141 tttttgaatt taatttaaat gttatctctt aaataattac ttaaatatcg tagcaacatg
     1201 ttctctgttg aacacaaata ttagtatatt catttttgta gtatacgtca acgacatttt
     1261 taaagttttt tgtgtaaaaa aacgcttctt attccctttt atcatataag tgtctaatag
     1321 ttgtcataaa tagtgttaaa gcatttaaaa aggtatagga gttataaagt ttacaacgcc
     1381 tataccttct gaaaaagaaa ttataaaagc ttgttacacc gcatattctt tcagtcagcg
     1441 actaccaata taacattgta gccctaagac attgcttgac gcctcaatta ccacaatttt
     1501 ttcaaaatca gcagctacct actgacacaa catagcaacc aaacattacc tatcgtgtca
     1561 tgtaatcttg catccgatct tgcaacgctg taaatgtttc gaagccatcc tcttctaaga
     1621 agtgccctcc atcttccacg attcgcaagt tcccctctaa tgcattcatt aaacgctggg
     1681 tttctttata tgaaacgtat ttgtcatttt tagaactcaa tccgtaaaaa ttgtcaactt
     1741 tctttttaat attatcgtaa tcaatggtta cattacttaa atcaatatct aaatctatat
     1801 tttctgcatc ttctttaaag cccgctatac taaaaaagcc ttcaatcggc tgatcaatca
     1861 tttcaatata ttttaaagct gtgattgaac ctaaaccatg tgttacaaaa tatgtatcct
     1921 ttttgcgtac attaatttgt ttcgtcatag cttcaatcca ctgatccact gtcttcgctt
     1981 caggggattc aaaattaaat aatgttacgt catatccttc taaagttaag ttatgctcca
     2041 accactgata ccaatgattt ctactatttc catgcataga atgtacaata attacatctg
     2101 tcatctcatt ctctcctttc aacttactac ttcttttcta tttttaaaaa aatgactgat
     2161 tacctataat tgtaaaataa aaacacctta attagaaatg ttatatcgca aagtgacatt
     2221 tctaattaaa gtgtattgtc atcatttcaa tatcattcaa aaacagctaa acctttgtct
     2281 ctgcttcaaa ttcacaaaaa taattcccgt tgaaagtatc tatatttaca cattacttcc
     2341 accattatat aacttaaaaa tgactatatt tcatcaaaca ttatctaaag gcgtcgcacc
     2401 tacaccacac catccaacaa ttaacttaca actctgcgat tacttcttca gcagcaactt
     2461 taccttgcgt aatacaatca ggtagtccaa ccgcttcaaa agatgcacca gttactctaa
     2521 gtcgtggata tgtttgttta atatgtgctt gaatctgtct aatttgttga atgtgaccaa
     2581 catggtactg tggcatactt ttcggcaaac gattgacaat tgtaaattca ggatcgcctt
     2641 taaatgtcat catttgactt aaatctctac gtacaatcga tactaattca ttatctgtat
     2701 gatcatcaac cacagtatca cctggtttac ctacatacgc acgaatcaaa accttacctt
     2761 ctggtgtagt aaatggccat tttttcgatg tccaagtaca tgcggtaatg tctgtatcac
     2821 tcgttctcgc aatcacgaag ccagtaccat cataagtatt ttcaatatct ttttcatcaa
     2881 atgccaatac aacagttgca acagtcgtac tatccatcgt tttaaagtaa tcaaatgctg
     2941 gatcttgtcc gaaccaattc aaaaagactt gatgcggtgt tgtcactaat accccatcga
     3001 atacatcttc ttgttgatta ctgtaaacaa ttttatattg cttttgagat gtaataatat
     3061 catccactga cgtattgtag cgtattgtca cacctttatt tttcacatct tgttctaatg
     3121 cttcaataaa tgaacttaaa ccatgcttaa attgtttgaa ttgtcctttt ggtgcgccag
     3181 gatataattg tctttgtttc agacgcttat ttttctcatc cttcatacct tttatcagac
     3241 ttccgaatgc ctcttctttt tctttaaaat taggaaacgt actcatcaaa cttaatttat
     3301 caatatcggt accataaata ccacccatta aaggctctat taaattctca agtacctcat
     3361 tacctaatct tgctctgaaa aatgcaccaa cagaaatgtc accatcttgc atttgtatag
     3421 gctttttgat taaatctaat cctgctctta atttaccaag tggtgaaatt aatttagttg
     3481 tcacaaacgg tttgatatct gtcggaattc ccataattga tccacctgga ataggataca
     3541 atttgttttt cgcaaaaata tatgattgtc cagtcgtatt tgtaacaata tcttgttcta
     3601 atccaatatc tttcgctaat tctgtcataa tcgtttttct acctaaataa gattcaggcc
     3661 ctagttcaat catataacca tctttacgat acgattgaat ctttcccccc ggacgattcg
     3721 atgcttcaaa gatggttaca tcaatattag gatcttgctg ttttaaaaaa tatgcacttg
     3781 ataaacctgt tatccccgct cctataatag ccactgattt agtcacaacg tttcacgctt
     3841 ctttcgtcat taaaatatag acttgatttc atcaacaatt gcaccgataa ataatggatg
     3901 tgtattcggc atttttggac gataataatt cgcaccaata tcatcgcaaa ctactttaca
     3961 ttcataatca ttgtcataaa gcacctctaa atgctcacat acaaaaccta ctggcgtata
     4021 tataaagttt ttatactgat gtttttcata taaatcacgt gttaaatctt gtacatctgg
     4081 ccctaaccaa ggtgtacctg tattaccttc agattgccaa ccaatcgcga tatgttcaat
     4141 attagattgt tctttaatta aaagcgcagt atgttctagt tcttgtggat atggatcatt
     4201 attcttttcg attaaacctt ttggcaaact atgtgccgaa acaactaata ccgtgtcttt
     4261 atgttcctct tccggtattt gagctaatgt ttcgttgact ttattcgtcc aatattcaat
     4321 aaatttaggt tgttcataat aatgtttcac atgtgtaagt tgaataccat attttgcagc
     4381 ttcttcatca gcacgtttgt catatgatcc tactgaaaat gaagaataat gtggtgctag
     4441 tactaccgtg attgcttcag taatgccatc attgtgcatt tgttcaaccg catcttcgat
     4501 aaatggtgaa atgtgtttta atcctaagta tagtttaaat tcaacatctg catatgcttt
     4561 atttaatgct gaaactagcg catcagcctg gtcatctgtt gtacctgcta atggtgataa
     4621 accacctata aattcatatc tatctttcaa atcttgaagt tcttcttcag atggacgttt
     4681 accatgtcta atatctgtat aatatggctc tatgtcactt tctttataag gtgtgccata
     4741 agccataact aataatccca tttttttagt cattgataat accttccttt aaatgaatta
     4801 tctttcatgt gcttcaatgt aaaactatga ttatctttgt gtatatgtgt gtacgaattc
     4861 gcttacttta cgtaacgtct ctggttgcac ttctgggaat acaccgtgtc ctaaattaaa
     4921 gatgtgttta ccgttctcca taccttgatc taatattggt ttcaatctct cttcaatgac
     4981 attccatggt gctaataaaa ttgatggatc taaattccct tgtaatgttt tagtaacgcc
     5041 taattgttga gcctgattaa tagacgttct ccaatctagg cctaatacat caatcggtaa
     5101 atcattccat tcattgatta aatgactggc acctacaccg aataaaatta ccggcacatc
     5161 atgtttttct ttaacctcac tgattaatcg aatcatatgt ggtttaatgt aacgtctgta
     5221 atcctcgaca tttaatgcac ctacccatga atcgaaaatt tgaatcaatt cggcacctgc
     5281 ttcgacttga gctgttacat atttaacaga tacatcaact aaatgattca ttaaagcaaa
     5341 ccatgttgct tcatctctat acatcatcgc ttttgtaaaa ttgtaatttt tcgatggtcc
     5401 gccttcaatc atatatgacg ctaatgtaaa tggtgcccca gtaaatccta ttagcggcac
     5461 atttaacttt tcttctgtta aaagtttaat tgtatctaat acatatggta catctcgttc
     5521 ggggtctatt tgagaaagtt tctcaacatc ttgaattgtt ttgattggat tatgaatcac
     5581 tggaccaata cccgatttaa tttctacatc gacaccaatt ggctttaatg gtgtcataat
     5641 atctttgtat aaaattgctg catctgtatg ataattatca actggtaaat gtgttacata
     5701 agcgcacaac tccggctgat gtgtaatatc gaatagtgaa tatttttctt tcaattttcg
     5761 atattctggt tgcgaacggc cagcttgtcg cataaaccaa acaggtgtat gtgatgtttc
     5821 ttcacctttg atcattttta aaattgtatt gtttttatta tgcaccataa aggcctccta
     5881 aattaaaatc attcttatct atattatcat atcgctcatt cgttcgtatt ttcaataaat
     5941 aaatgtcata aaactgacat ttaatcatag aactatttat tgtaaattta aattctaaag
     6001 tccattattt tgtatcatta cttctaaata tctcgcaaga ttcatt
//
LOCUS       DBNENW010000147         5995 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNENW010000147 DBNENW010000000
VERSION     DBNENW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.149"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            71..1015
                     /locus_tag="AC1LW8_002253"
     CDS             71..1015
                     /locus_tag="AC1LW8_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774049.1"
                     /translation="MRKIMRILLCASIIVSGCSFIPKNQTKSIPKTVSVKDYDGKYIG
                     EHKKRNEVFLKKHKDEAIKKYKDYVKDTFGYDCKINLVDAYTNKSGFSEKSKVDGISV
                     VGTVNYDVPFQLQLLFVESGNGITISTFTPGHINETSAAVSAMMYKRYEHEIEQARLK
                     LKSEIEKDSYYAMSEKLQVKQEFNGVTRQYLNFRTDSIDDLDKFKKEFKPVMHLKGDA
                     FNQQLQSLINKYPQIQKNMKSEFIAYYDKEKNRETVKNYAWNLQKSINDIMQSYPSTK
                     FVQFYKDDVSPSMVDGNGRLKSDTNVISIEGGKYDENK"
     gene            1002..1304
                     /locus_tag="AC1LW8_002254"
     CDS             1002..1304
                     /locus_tag="AC1LW8_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774050.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTAPLKNNLTKLKEQLDKTTH"
     gene            1458..1817
                     /locus_tag="AC1LW8_002255"
     CDS             1458..1817
                     /locus_tag="AC1LW8_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774051.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1902..2513)
                     /locus_tag="AC1LW8_002256"
     CDS             complement(1902..2513)
                     /locus_tag="AC1LW8_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIP4774052.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSHTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(2592..3068)
                     /locus_tag="AC1LW8_002257"
     CDS             complement(2592..3068)
                     /locus_tag="AC1LW8_002257"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIP4774053.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3071..3571)
                     /locus_tag="AC1LW8_002258"
     CDS             complement(3071..3571)
                     /locus_tag="AC1LW8_002258"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774054.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(3806..4573)
                     /locus_tag="AC1LW8_002259"
     CDS             complement(3806..4573)
                     /locus_tag="AC1LW8_002259"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIP4774055.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFSTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(4570..5262)
                     /locus_tag="AC1LW8_002260"
     CDS             complement(4570..5262)
                     /locus_tag="AC1LW8_002260"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HIP4774056.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5279..5941)
                     /locus_tag="AC1LW8_002261"
     CDS             complement(5279..5941)
                     /locus_tag="AC1LW8_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIP4774057.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2024 a         1060 c          874 g         2037 t
ORIGIN      
        1 caattttttg atttttgtgt aatttatcta tcaactgaaa aacagctaat acaaaggagc
       61 taatgaagat atgagaaaaa ttatgcgaat cttattgtgc gcatcaatca tagtaagcgg
      121 ttgctctttc ataccaaaga atcaaacaaa atctattcca aaaacggtta gcgtcaaaga
      181 ttatgatggt aaatatattg gtgaacataa aaaaagaaac gaagtatttc tgaaaaaaca
      241 taaagatgaa gcaatcaaaa aatacaaaga ctacgtgaaa gacacctttg gttatgattg
      301 taaaattaat ttagttgatg cctacactaa taaatctgga tttagcgaga aaagtaaggt
      361 agatggtatc tcagtggttg gtactgtcaa ttatgacgtt ccttttcaac tccaattgct
      421 ttttgttgaa tctggaaatg gtataactat ttcaacattt acacctggtc atataaatga
      481 aacatcagca gccgtttcag ccatgatgta caaacgttat gaacatgaaa ttgaacaagc
      541 tcgattaaag cttaaaagtg aaattgaaaa agacagctat tatgcgatga gcgaaaaact
      601 tcaagtaaaa caagaattta acggcgttac aaggcaatac ttgaatttca gaacagatag
      661 tatcgatgat ctggataagt tcaaaaaaga attcaaacct gttatgcact taaaaggtga
      721 tgcattcaat caacagttac aatctttgat taacaaatat ccacaaatac aaaaaaatat
      781 gaaatcagag ttcattgctt attatgataa agaaaaaaat agagaaacag taaaaaacta
      841 tgcttggaac cttcaaaaat ctataaatga cattatgcaa tcatatccta gcacaaaatt
      901 tgtacagttt tataaagatg atgtttcccc gtcaatggta gatggaaatg gccgtttaaa
      961 atcggatact aatgtaattt ccatcgaggg aggaaagtat gatgagaaca aataatcata
     1021 taaccgattt agatgctatt gagttaagta gcggttggaa ttacaattcg aaattggaga
     1081 ctggcaaaaa tgcactacta tcagatagcc ttttaagaga tattaaagta ttaagttcta
     1141 atgctaaagg cgacgctgac atactagaaa cagaacttcg tgctgtaata aacgcaatgt
     1201 ctcaaaacga aggtaaatct gttgatgcac ttaaaaatag taccgctcct ctaaaaaaca
     1261 atctaaccaa attaaaagag caactcgaca aaactacaca ttaatttcat acattttgag
     1321 tgaacagaat tatatttcca tagcaaacat tcctaaacta cacatacgtt acataattga
     1381 ttcattttta tagaaacggg taaaaatgat aaagtaatca tctttcacaa aaaatcaaat
     1441 attgggagga attaaatatg attttgcgtt acgcaacgaa tttaaaactt gcgaaagagc
     1501 tataccaagc cagtaaacct aaattgcaag aagataaggg tatgattgaa ctgtttgaac
     1561 atacttttgg cttgcaaaaa gaacttgtaa aatacgtcgg cattgcagaa gcaactactg
     1621 ctgctttata ctcagctagc tttatcaata aaaacatttc tcgccttgca tcactatcaa
     1681 cgataggcat tttaagtgta gctgcttaca aacatttcga agctggccaa ggtaaaaaag
     1741 gtgcacaaca tgcattagat ttattaggtc ttgctacttt aagtttacta gatacatttt
     1801 cttgtaataa aaaatagtct tcattttaga agcacatact ttagcacctg tgtacttttg
     1861 attcaatcca taaacggtac accgctatag catgtgtttt attatttttc tggtctcttt
     1921 atcgtaaata ttccccctaa tttagcataa tcatttccgg ctaaacgaga aacagcctgt
     1981 aatgcgtgtg gattgatttt aaaatcaccc tcataaatac tgtcatcaat atgataagtt
     2041 acaatttcac ctattattaa atcagcccca tctaatacat ctccaagcaa tatcatttgc
     2101 gatagtttac attcgaatct cattttcgca tctttaattc ctggcgtctt aatcgttgta
     2161 gatgttaaaa gtgataattc tgtatgactc aactcactgt caccatatgc taacggcgct
     2221 gcagtctcat taatatcttg aacattatct tcgtctgtaa tatgcacaac aaagtctcca
     2281 gtccgttcta tatttaatgc agtatctttt ctcttacctc ctgcacgttg aactgcaata
     2341 gcaatcattg gcggatgatt attaacaata ttaaaaaagc taaatggtgc tgcatttact
     2401 gatgcatctt gatttaatgt tgtaacaaaa gctataggtc gtggaataat tgaaccaatt
     2461 aataatttat agttttctct agcagttaat gattgtgcat caaacgtata cataatacct
     2521 acctcttttc taagtatatc taggtatttc tccgattttg gttaatttaa acatctattc
     2581 tcctctgaaa atcacttgta tttatttagc aaatcttttg aaatatgaca catatgcata
     2641 tcttctggat atttttctaa atgttgctga tgttcttcag cacttttaat gtagttagac
     2701 agcggtaaga cttccactgc aatttgatct ctgtctttac gtcgttcaat gaactgacgc
     2761 gcttcaatta agtggtcatc tacacaacta tataaacccg ttcgatactt ttgtccaata
     2821 tcatttcctt gttgattcac actgtaagga tcaatgattt caaataaata attcataatg
     2881 tctgtaattg ttaacatacg atcatcgaaa tgaagtttga cacattcagc ataaccatca
     2941 tacggaccgt ctaatttaga acttcttcca tttgctcttc ctgcttctgt atgtataatt
     3001 ccaggtattg ttgcaaaaaa tgcttcaaca ccccataaac atcctcctgc tacataaaca
     3061 actgccatat ttacacctca tcatcctttt ttatattttt aacaaggtta taccatttaa
     3121 taccgccatg acatgattct gatacacctt cattacgata cccatatttt tcataaaatg
     3181 aaattaatga ttctcgacat gttaacgtta caccatgtcg atgatgattc ttagcaagag
     3241 tttcaaaata gtttagtaag cgacctgcaa taccctgacc ttgataattt ggtgctacaa
     3301 caagacctaa cacactaata tagccacctt cactattatt tgtggagaca tttttaaata
     3361 aatcatcgct aatgtaacgc tcttttatga ctggaccgtt gatgtatcca attacttcat
     3421 cattcttttc agctacaata aaagtttctt gaatttgttc aattctcaat ttcaatgcct
     3481 ctcgtgttgc agcttcttcc ggtgaaaaac caatattttc aagtgcaata atttgatcta
     3541 agtcctgcat gattactttt cttaatacca taaaaacact tccttagccc tatatatcaa
     3601 aagtaatacc ttcatcttta tattcattat tttcaaacta ccttcactat aaaaaacgag
     3661 tctttttatt gaaagccatt cgccttaatc aattagacaa gttgtattat acgaattagt
     3721 aaataatcat gattataatt cattttaagc atactcaaaa agactggtac atgtatacca
     3781 gtctaattcg aaaaaatata tttagttaaa accatttcaa aaacgacttc acttttcctg
     3841 gcttagtcgg tctgtctaaa taaacaaaat tatcattgat tactgaactg gcatttcgaa
     3901 gtaatgcttc aatatcattc gaatatttct tcaatttatg attgtgaaat aattcttgca
     3961 tcaaaaatgg tctttggtca catgaatgtg catctgaagc tacaaaatga gccaaattac
     4021 attctataaa ttgtaatgat aacttttgaa tgtttttacc aaatccacca actaaagaac
     4081 tcgatgttaa ttgactcaat gccccatttg caaccaattc atataatatt tccggatttt
     4141 tggcgatact tctatttctt tcaggatgtg caatgattgg tatgtaacct ctcgattgta
     4201 tttcaaaaaa caattgtttt gtataatgtg gtacttcgcc cgttgaaaat tcaattaata
     4261 aatatttcga acgattaata ccttgaatac taccattatc taagcctttc agaatcgaat
     4321 ctgtaattct aatttcttgc ccgggaaata atttaatatc caatgcttga acttctggat
     4381 gcgttcttaa ctccgccaat ttcacaagca cttgttgaaa tgtattatca tatctcggat
     4441 gcaaatgatg aggtgtcgct acaatacttg ttacaccttc atccttagct tgctttaata
     4501 gtgcaatact cttttcaatt gttttaggac catcatctat atcaactaat atatggttat
     4561 gaatatcaat catgattcat cagtcccata atatgcatag taactagcac ttttatcttt
     4621 aggcattcta tttaagacta cacctaataa tttagcacct gttgcttcaa taagttcttt
     4681 tcctttttta acttcatctt tattattatt ttccgaatta actacgtaga caacattgcc
     4741 ggtaaacttt gaaaataatt gcgcatctgt aactgtgttc actggtggcg tatcgataat
     4801 tacaaagtta taattcatca ataatgtgtc atacaaattt gcaaatgccc ttgatgtaat
     4861 taactctgac ggattcggtg ggattggccc agacgtcaag acgtctaaat cttgaatttc
     4921 agttgagata atactgtctt gataagttga ccaatttagc aataaacttg ataggccttc
     4981 attgtttggc aaattaaaaa tataatgctg cgtaggttta cgcatatccc cgtctacgat
     5041 tagtgtttta taacctgctt gcgcatatgc aactgctaaa tttgctgcaa ttgtagactt
     5101 acctgcgcct ggtgcctctg aagtgattac aatgctttga actgcactgt caggatttgc
     5161 aaacataata tttgatctta tgcctcgaaa tttctcgcta ataggtgact ttggttgttc
     5221 atggacaatt aaacttgatg tacttcttcg tgtattcgtc atggtaattc ctcgtaaatt
     5281 aaaatttttg tattgaacct aaaataggta atcctagttg cgattcaaca tcttcttctg
     5341 tcttaatacg cttatctaat aattctttta agaaaataat caatattgct aaaacaatac
     5401 caacaataat gctgataact aagttgacag atactattgg agatactttt acagcattat
     5461 catgtgctga ggaaagtatc gtaacattat caacactcat aattttaggc atgtcatgag
     5521 caaaaacttt agatatttta ttaacaattt tgtcagattc agatttattc ccagtggtaa
     5581 ctgatacagt aataatttga gagtttgttt gattggttac ttttaaaaat gaattcaact
     5641 cagctgttga atactgacca tcaaattctc tagatacttt atctagaatt ctaggacttt
     5701 tgataatttc cgtatatgta ttaacagact gcaaactact ttgaacattt tggaaagcta
     5761 aatcacttga ggactttttc atgttcacta atatttgagt agaagcagta tatttgtcag
     5821 gcataacaaa aaaggttaat gccgcactta ctacaagaca tattgccggt aaaataagca
     5881 ataatttaat attcttcttt agaatattta atagttttac taaatcaaac ttttctttca
     5941 tggtttcctc cacataatca atcattgtat tcattatgta tgttttataa atcgg
//
LOCUS       DBNENW010000148         5861 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNENW010000148 DBNENW010000000
VERSION     DBNENW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.150"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            397..1308
                     /gene="rapZ"
                     /locus_tag="AC1LW8_002262"
     CDS             397..1308
                     /gene="rapZ"
                     /locus_tag="AC1LW8_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIP4774058.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1305..2300
                     /locus_tag="AC1LW8_002263"
     CDS             1305..2300
                     /locus_tag="AC1LW8_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HIP4774059.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2411..3355
                     /gene="whiA"
                     /locus_tag="AC1LW8_002264"
     CDS             2411..3355
                     /gene="whiA"
                     /locus_tag="AC1LW8_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIP4774060.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3928..4515
                     /gene="clpP"
                     /locus_tag="AC1LW8_002265"
     CDS             3928..4515
                     /gene="clpP"
                     /locus_tag="AC1LW8_002265"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIP4774061.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4758..5660)
                     /locus_tag="AC1LW8_002266"
     CDS             complement(4758..5660)
                     /locus_tag="AC1LW8_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIP4774062.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGRLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         2103 a          821 c         1085 g         1852 t
ORIGIN      
        1 gcacattatc gtaagctgac ttttcgtaag cttctgtgtt ggggccccgc caacttgcat
       61 tgtctgtaga aattcttttc gaaattctct gtgttggggc ccacacccca actcgcattg
      121 cctgtagaat ttcttttcga aattctctgt gttggggccc ctgactagag ttgaaaaaag
      181 cttgttgcaa gcgcgttttc attcagtcaa ctactagcaa tataatatta tagaccctag
      241 gacattgatt tatgtcccaa gctcctttta aatgatgtat atttttagaa atttaatcta
      301 gacatagttg gaaataaaaa taaaacatcg ttgcttaatt ttgtcataga acatttaaat
      361 taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga aaaagaaaaa
      421 agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc tttggttatt
      481 caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt gttattacct
      541 aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc aattgcaatt
      601 gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa agttaaaagt
      661 gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa attaatttca
      721 agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag atccttaatt
      781 aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc taattttgtt
      841 atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg atactatgaa
      901 gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa acatgggatt
      961 cagatggatg cagatttagt atttgatgta cgatttttac caaatccata ttatgtagta
     1021 gatttaagac ctttaacagg attagataaa gacgtttata attatgttat gaaatggaaa
     1081 gagacggaga ttttctttga aaaattaact gatttgttag attttatgat acccgggtat
     1141 aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg acaacatcga
     1201 tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga atataatgtt
     1261 tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga caaataaaag
     1321 ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta agagaattcc
     1381 caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca gggaaaatca
     1441 gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca gctttaagtg
     1501 attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat caaattagcg
     1561 gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat gatttcggac
     1621 atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt ccatctacaa
     1681 atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt ggagaaacaa
     1741 atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac gatgtgcaac
     1801 caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt ggaccagggt
     1861 cattatatac gagcgttatt tctaacttat gtgtcaatgg tatttcagat gcgttaattc
     1921 attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg gaaacagatg
     1981 gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa ccgtttattg
     2041 attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa tatgaagaaa
     2101 aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc ataaatgtaa
     2161 aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat aatactaaag
     2221 tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt cctttcgtac
     2281 caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata atagagctgt
     2341 gtaaaaaaat gaaaatagac agtggttcta aggtgaatca tgttttaaat aagaaaggaa
     2401 tgactgtacg atgagctttg catcagaaat gaaaaatgaa ttaactagaa tagacgtcga
     2461 tgaaatgaat gcaaaagcag agctcagtgc actgattcga atgaatggtg cacttagtct
     2521 ttcaaatcaa caatttgtta taaatgttca aacggaaaat gcaacaacgg caagacgtat
     2581 ttattcgttg attaaacgtg tctttaatgt ggaagttgaa atattagtcc gtaaaaaaat
     2641 gaagcttaaa aaaaataata tttatatttg tcgtacaaag atgaaagcga aagaaattct
     2701 tgatgaatta ggaattttaa aagacggcat ttttacgcat gaaattgatc attcaatgat
     2761 tcaagatgat gaaatgagac gcagttactt gagaggagct tttctggcag gtggctcagt
     2821 gaataaccct gaaacatctt cgtaccattt ggaaattttt tctcaaaatg agagtcatgc
     2881 agaaggctta acgaaactaa tgaatagtta tgaattgaat gccaaacatt tagagcgaaa
     2941 aaaaggaagt attacgtatt taaaagaagc ggaaaagatt tcggattttc ttagtttgat
     3001 aggtggctat caagcgttat taaaatttga agacgtacgt attgtaagag atatgcgtaa
     3061 ttctgttaac cgactcgtta attgtgaaac ggcaaatcta aataaaacag ttagtgctgc
     3121 gatgaaacaa gttgagagca ttaaattgat tgataaagaa attggtattg aaaatttacc
     3181 agacaggttg agagagattg ctagaattcg agtagaacat caagaaattt cgttgaaaga
     3241 gcttggagaa atggtatcaa ctggtccaat ttcaaaatca ggtgtaaatc accgattaag
     3301 aaaacttaat gatttagccg ataagattag aaatggtgaa caaatagaat tataagtaag
     3361 aaggtgtttt tggtagtgaa ttatcaaaaa cactttttta tgtttaaaaa gtgtttaatg
     3421 aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa attcaataac
     3481 caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg tcataagaaa
     3541 atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca aaaacaaaat
     3601 cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta ttgtatttta
     3661 ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa tcttacgtgt
     3721 tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg agaagtaaaa
     3781 taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag cgtcaaacaa
     3841 tgtaactatt taaagtcaaa gtgtttgacc aagtttgact taatatgtaa aataatgagt
     3901 aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat tgaaacaaca
     3961 aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg tattattatg
     4021 ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt attattctta
     4081 caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg tggaagtgta
     4141 acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt tcaaacaatt
     4201 tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc aaaaggtaaa
     4261 cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg tgctcaagga
     4321 caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga aaaattaaac
     4381 cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga cacagatcgt
     4441 gataacttct taactgcaga agaagctaaa gaatatggct taattgatga agtgatggta
     4501 cctgaaacaa aataattcaa agtaaagagt agactaagca gtctgctctt tttgtatgag
     4561 taaaccgagg tgtcaataaa ttgtttacta tactttgagc ggaaatatga ttgaatgaag
     4621 ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa aggaacattt
     4681 caatcctttg tttgtaagtc gctctagaca ttacatttag tacatatgtt gtttctaatg
     4741 ctcattaatg gtattgatta ttctttaatt aaatcttcaa gtgccatttt taaattacta
     4801 tatttaaatt ggaatcccaa tgcttgaatt ttattaggta atactttttg agtatccaat
     4861 actactgttg acatttgacc aagtatgaga cgcattgcaa gacttggtgc ccaagtttca
     4921 tggggcttat gcatagctct tgctaaagtg tagccaaata aattttgacg ctcaggtata
     4981 ggtgcagtta aattaaacgg accactagct gactcgttat ttattaaaaa taaaatagct
     5041 tgaattaaat cattgatatg aatccatgaa taccattgtt gaccagaacc taatctacca
     5101 ccaatgtaat atttgtatgg tagtttcatt gtttgtaacg caccgccttc attcgataaa
     5161 atcataccga aacgaccgat gacaactcgc gtacctaatt gttcaaattg ttgtgcgaaa
     5221 cgttcccatt gatacacaat atctgataag aaatcaaatg gtaaagtttt ataaacttct
     5281 gtgtaactca taaataaatc aggaggatag taaccagtgg cgctagcatt aaataaaact
     5341 ttaggtgctt tattacgtga tttaaacaat tcatataaag cttgcgtaga ttgaattcta
     5401 cttagcatta gcgtttgttt atattccggt gtccatcgtt tattcagtgt agcacctgct
     5461 aagttgatga ccacatcgat attttgagga actttgtgtt cccacccaga tttagcccag
     5521 ttgacatatg aaattttctt atcatttgaa atttggtcgt gtcgcgttaa tatcgtgata
     5581 tgtgaatctg attttttaat ttcattaact aattgagatc caaccatacc agtcccacca
     5641 gtaattaagt attgtttcat tatcattcac cccatgtaat tttgtattta gttactattt
     5701 aataatcatt attgttgttc aaaggttata cattatttag acaataatat gtcaataact
     5761 ttttttgaat tgtattttaa taaaaatgat ataagttata ataaaggtgt accttcgata
     5821 acgaataaac atctcttaaa agtatgtgta aaacgctgca t
//
LOCUS       DBNENW010000149         5792 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNENW010000149 DBNENW010000000
VERSION     DBNENW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.151"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..354)
                     /locus_tag="AC1LW8_002267"
     CDS             complement(<1..354)
                     /locus_tag="AC1LW8_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIP4774063.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKII"
     gene            complement(272..571)
                     /locus_tag="AC1LW8_002268"
     CDS             complement(272..571)
                     /locus_tag="AC1LW8_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HIP4774064.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(558..991)
                     /gene="comGD"
                     /locus_tag="AC1LW8_002269"
                     /pseudo
     CDS             complement(558..991)
                     /gene="comGD"
                     /locus_tag="AC1LW8_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
     gene            complement(969..1280)
                     /gene="comGC"
                     /locus_tag="AC1LW8_002270"
     CDS             complement(969..1280)
                     /gene="comGC"
                     /locus_tag="AC1LW8_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HIP4774065.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1294..2364)
                     /gene="comGB"
                     /locus_tag="AC1LW8_002271"
     CDS             complement(1294..2364)
                     /gene="comGB"
                     /locus_tag="AC1LW8_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HIP4774066.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2336..3310)
                     /gene="comGA"
                     /locus_tag="AC1LW8_002272"
     CDS             complement(2336..3310)
                     /gene="comGA"
                     /locus_tag="AC1LW8_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HIP4774067.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(3362..3985)
                     /locus_tag="AC1LW8_002273"
     CDS             complement(3362..3985)
                     /locus_tag="AC1LW8_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP4774068.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESGKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3982..4311)
                     /locus_tag="AC1LW8_002274"
     CDS             complement(3982..4311)
                     /locus_tag="AC1LW8_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HIP4774069.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4311..5297)
                     /locus_tag="AC1LW8_002275"
     CDS             complement(4311..5297)
                     /locus_tag="AC1LW8_002275"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIP4774070.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5294..5497)
                     /locus_tag="AC1LW8_002276"
     CDS             complement(5294..5497)
                     /locus_tag="AC1LW8_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HIP4774071.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5478..>5792)
                     /locus_tag="AC1LW8_002277"
     CDS             complement(5478..>5792)
                     /locus_tag="AC1LW8_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774072.1"
                     /translation="DNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERG
                     LRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNADYINLEKELTKSNESK
                     NK"
BASE COUNT         1877 a         1010 c          742 g         2163 t
ORIGIN      
        1 aataatttta ttattaatta atttgtattc gatcatttct tcacctttat gtaaaatgat
       61 acggtgttgc ctaatttcaa tatcgtttct atctactcct ttaaaatcat ctagaatatc
      121 tcttgagaaa aattcgaaat ctacagttgt taaatcccta ctttcaatta gaaaagtttt
      181 actaagtcta attaagtctg gaacaattag taaagttata cttataacca tcatcgctac
      241 taacatttca atgagcgaaa aagctttgac attaatactg tatacatgtc ttttgataag
      301 aagtggtatt ttttgaaata gcacaaattt gttggtcact ttgcttaata tcatacttcc
      361 ctatagttac ccccttctta agttcttctt tattaatttt agatacagtc gtcaaaatta
      421 ctttagaagc atcaattgtt tgtagtttat ggtttatact cgcttgcatt tgattcatca
      481 ttggtatcaa tagtaatgta atcaatccaa ttagcaaaaa tccagccata ctatctatta
      541 agaatgaacc tttacactta tagcttttca taacgaattc ttcctttttc aatatggaat
      601 attattctat aaattgaatt gttatgctac caaatttatt gatattccct tttttatcaa
      661 aggcaataat atcaactttt gcaacattaa ttattttgcc tacttttaat tttagaaatc
      721 gtattttatt attctctatt actttaatag tgtcactgtt ttcataaaat ctaacattga
      781 tatatccttg atttgctata gcttgcgact taatataatt caattcagta ataaaagaaa
      841 tgatatttgc ctcatcatct attactctaa gattgcttaa tcctttagat gtcattgtca
      901 aaagtagaaa tatactgatt aacatcatta ccacaagcat ctcaatcata gtaaatgctg
      961 actgctttct aatttgcaac tgcttctcca ttactaattg tgattgtctc tcctgattta
     1021 catgtctttt gtgcttcttt tataaaacca tctgcaatta agtcttcaat agacgatgga
     1081 tttctattat gtttcaatgc atacgcttca atttgactat taaccatttt tacctgtgca
     1141 ttacaacctg ttgattgtat gtgagcagtt tgtttagcaa tatttggaat gattaaaatt
     1201 aataataaac tgatgattaa taacactaat agcatctcta tcaatgtaaa cgcttgagtt
     1261 ttcttaagaa atttaaacat atattaacct ccattatttt atactttgca tcatttgaaa
     1321 cattggtaac ataattacta aataaattgc gacaataaat aaacctaaaa tcaaaaataa
     1381 aataggctgt aaaaactgag tctgttttat cgctttatct tctatttgtt ttactaatat
     1441 ttgtgaatat aactttagtt ctacttctag cttccctctc ttttcacctt gtagcacaaa
     1501 cttaattaat tgaggcttaa agcattttag tttttctaaa atttgaggca aaccatatcc
     1561 catttctgaa taagttaata agtatttacc tagaaactgt ctaaatggat cactactatg
     1621 gttaatataa acgtctacta ttgattgaag tgtaatacca tttttataaa acaacactaa
     1681 ttcattagtt acaaaataag ttttaaataa ttggaaatag cctgatatta gcggtagttt
     1741 catcacaaag tttatcttat tgagcatatt taaattgtta taaattaatt tcataataat
     1801 agccaacata gatactatta tgagcattac tacaattata gtaggtaagc tggtaatgaa
     1861 aaaagacaat gttttttgaa aagaagatag ttgaatattc atagaagtat ataattgttg
     1921 aaactgtgga attactgtga ggtttaatat aataatcata gcaataaaga tagaaactag
     1981 tattaagggg tattgcagtg tctttaacaa tcgttgttct gactttctat tcactttcat
     2041 ataatttacg gtttcttcta gaacgtctat aatattgcca aatctttctg ccaaatatac
     2101 ttgcatgacg atagtatcgc tataacctat cagtgataat atttgattgc atggtgcacc
     2161 atttgaaatt tcacttagaa tggtggtacc taattgctta tttttatatg tcatttgaag
     2221 atttaaaaat tgaaaacttt gatacagagt gaaaccatat ttcaacaaat tacataaatt
     2281 tgaaagtaaa tcgatttgtt gggccttact taattgtcgc ttcttagaat gtagtttaaa
     2341 tgtatttatc cattgtagtt tcacaaatga cacctgcttt tgtcatatca tcaagtttat
     2401 cttctaagtt cttaaatgat gatggtaatg aatgattatg ggaaaagaaa tatcggagtt
     2461 gttgctgaga tagaatttcg catactaatt gtcgctgttg cttaatagta gttacaagtc
     2521 gttggtttat aattaagtta gttgcctgta tcaattcttg tacagaaatg cccatttcta
     2581 atagccttaa aatagcacct ttacaatcag ttgcatgcaa tgtagtcaga acaaggtgac
     2641 cacttaaact agcctgtata acacacttgg caacatcttt atctctgatt tcacctatta
     2701 aaataacatc aggatcacat cttaaaatag ctttaaacga atttacatag ttaatgccag
     2761 ctttatcatt cacattaatt tggacgatac caggaatttg catctctaca ggatcctcta
     2821 tagaaattac atttaaattc aaggctttat tcgcgtatga gaccatttga tacattaatg
     2881 tactctttcc tgaaccagtt ggcccactaa acaatagtaa tccttgtttc ttattcatga
     2941 ggtgtttaaa atcattgaat ttataagttg atttttgttg ttgaaaaaat tgaggtacaa
     3001 ttctgataac acaactttct tggccaagtg acaatggtaa agttgatatt ctcaaaaaat
     3061 atattttatt gaaatggtaa ctatatcgac cgctctgtgc gacttgctgt gtagaaacat
     3121 caagcccagc ttgaaacttc atataaacta ataacttttg ataaatgcta ttcccaattt
     3181 gttcatactg ctccaagtta tcattaattc taaatttaat acttacttca tttttaactg
     3241 gaataaaatg tacatcactc gctttcattt ctatcgcttt attaattatt tcttgaaata
     3301 gaatcttcaa aaaaacacct cctacatata atcacgtagg aggtgttttt attacttcaa
     3361 tttaaccgtg taaaaatgga tttaattgtt catcatcaac cgtcgtatat ggaccatgtc
     3421 cagggaataa aggtaaatcg ccttctaatt caaatatttt atcttgaata gaatcaacta
     3481 gcgtttcata atcaccttta tataaatctg tacgtccgat tccattatta aataatgtat
     3541 ctccaacaac tgcgaattca tcgaacacat atgttaaact tcctggtgaa tgtccaggtg
     3601 tgtgtaacac attaaactta aatccttcta tttctgtgct accttcgttt aacttttcag
     3661 gagttacctt acttgtaata attggtaatc catattgctt aaatttatct gccccatttt
     3721 taacgggatc ttttagaaaa tcaaactctg cttcatgcat ataaaccggg acatcgaatc
     3781 gatcaactat atcatcgact gctccgatat gatcaaagtg tgcatgtgtt aataaaatag
     3841 cttttaacgg tttatttatt tggtttaatt ttttaataat ttttccactt tcacctgaag
     3901 ggtcaatcag aataacagct ttgtcatttt cgatgaaata cgtattagta tcaactaagc
     3961 ctaaagttaa gcttgaaatc ctcataggtt ttcaccacta ttttctaaat gtttttgtac
     4021 tgatgttagt ttatcattca tttttctaga tttgtctcgt cggtcatcaa tacggatatt
     4081 tgtacaaact ctacttaaac ctttatcaaa aggtaattca tgtatcactt gcacaacttc
     4141 taatacatcg cttaattcac cttcaattag agtattcatt ggtgttaatt gaaaatcaat
     4201 tttacccatt gctttatatt cttgaagttt tttctgaata tctgcaatat atttactaac
     4261 actcggacct tccgttccaa ctggaataac aaccacatca acaatagcca ttattttacc
     4321 ccctctttat ctaatacata ggtcttgatt aatcctgctg ctcctgtaat acctgcatca
     4381 ttacctaatt tcgcttgtac aatttcagtt tcaaattgag caggtgcaaa tgttaaatta
     4441 tgatattctg ttttaatatt ttcaattaaa ataggtcctg cagtagacat tcctccacct
     4501 agaacgatat atttcggatt acttgtaaca ctaataatac tacataaata tccaatatag
     4561 tttgcaacct tttcagtaat gaaaatacag aattggtcac cagcttttgc cgcatcaaaa
     4621 acagcttttg ctgtaacctt attttcttta atcaattcta atatagaaga tctaaacgtc
     4681 aacttcggat agtagaagtt aactaagtta acaacgcctg tcgctgaagc aactgtttca
     4741 atacatccag aacgaccaca attacattta aatcgttgat cgaagtctgc tctaaaatga
     4801 cctatttctg cgccagagcc attatgacca tgtacgattt caccatttga aataattcct
     4861 ccacctagac ctgtaccaag tgtgatggca acaacatcat cggcaccttc accagcacct
     4921 ttgtgtttct cccctaaagc agctatgtta gcatcattat ctacatacac tggacaatca
     4981 acgaattgtt caaaaatctc acgtacatta actttttctg gccaatataa gtttactgct
     5041 ccatttactg tacctttttc aaagtcaaca ggacctggta caccaatacc tacgccaagt
     5101 acatttgaaa aattataatt attttcattt actttttcaa caaacgaatc ataaattcct
     5161 ttcaaaagtg tatatcctgt actatcagat gtatcagtgt gaatagacca tttatgtaat
     5221 tgttctaatt caggtgtgaa aatacctaat ttacaagtcg tcccgcctac atcagctgct
     5281 aaaataattt ttgtcatttc tgttcattcc ttctctgatt aattatcaac gtacatttca
     5341 aatattcttc cttagataac agttcatatt gatacaatga tgaaatctcc tgttgaatca
     5401 tttcgtacat atcttctgga tttttaaaat atattagaaa tccgtaactt ttcaataact
     5461 gttgtacatc ataaaagtta tttatttttc gactcatttg atttagtcaa ctctttttct
     5521 aagttaatat aatctgcatt tttagggtct gcatttaatg ctttacgcac atattttaat
     5581 gctttttcat catcatttaa tgaacgattt gctatcgcta actcaaaatt taataaacct
     5641 gatttaggaa acattctaag tcctcgctcc cattctgtca taccttcgga cttagaattg
     5701 atagtagcca taatcatacc acttaaataa tatgtttgat catcggcata atttttattt
     5761 attgtctgct tcacaatatt ttgagcatta tc
//
LOCUS       DBNENW010000150         5660 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNENW010000150 DBNENW010000000
VERSION     DBNENW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.152"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            73..1473
                     /locus_tag="AC1LW8_002278"
     CDS             73..1473
                     /locus_tag="AC1LW8_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774073.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENVDIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYIDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2017..3393
                     /locus_tag="AC1LW8_002279"
     CDS             2017..3393
                     /locus_tag="AC1LW8_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIP4774074.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3395..4534
                     /locus_tag="AC1LW8_002280"
     CDS             3395..4534
                     /locus_tag="AC1LW8_002280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIP4774075.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4509..4874
                     /locus_tag="AC1LW8_002281"
     CDS             4509..4874
                     /locus_tag="AC1LW8_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HIP4774076.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            4877..5146
                     /locus_tag="AC1LW8_002282"
     CDS             4877..5146
                     /locus_tag="AC1LW8_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774077.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5347..5514)
                     /gene="vraX"
                     /locus_tag="AC1LW8_002283"
     CDS             complement(5347..5514)
                     /gene="vraX"
                     /locus_tag="AC1LW8_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1q-binding complement inhibitor VraX"
                     /protein_id="HIP4774078.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         1964 a          827 c         1046 g         1823 t
ORIGIN      
        1 attcttataa atttgacaaa actaattaac tccgtataat tatgaaacat acaagaggga
       61 gtgtatgaat tcatggattt taataaagag aatattaaca tggtggatgc aaagaaagct
      121 aaaaaaaccg ttgttgcaac cggtatcggt aatgcaatgg aatggttcga ttttggtgtc
      181 tatgcatata caactgcgta cattggagcg aacttcttct ctccagtaga gaatgtagac
      241 attcgacaaa tgttgacttt cgcagcatta gccattgcgt ttttattaag accaattggt
      301 ggtgtcgtat ttggtattat tggtgacaaa tatggacgta aagttgtatt aacatctaca
      361 attattttaa tggcattttc aacattaacc attggattat tgccaagcta tgatcaaatt
      421 ggactttggg caccaatact attattgctt gcaagagtac tacaagggtt ttcaacaggt
      481 ggagagtatg cgggggcaat gacatatgtt gccgaatcat ctccagataa gcgtcgtaac
      541 tcattaggta gtggactaga aattgggaca ttatcaggtt acatagctgc ttcaattatg
      601 attgctgtat taacattctt tttaacagat gaacaaatgg catcatttgg ttggagaatc
      661 ccattcttac tcggtttatt cctaggatta ttcggcttat atttacgtcg taagctggaa
      721 gaatcaccag ttttcgaaaa tgatgttgca acacaaccag aaagagataa cattaacttt
      781 ttacaaatca tcagatttta ttacatagat atatttgtat gttttgtagc tgttgtattc
      841 ttcaatgtta caaactatat ggtaactgca tatttaccaa cctatttaga acaagttatt
      901 aaattagatg caacgacaac aagtgtatta attacttgtg tcatggcaat aatgattcca
      961 ttagcattaa tgtttggtaa gttagcggat aaaataggtg aaaagaaagt atttctaatt
     1021 ggtactggtg ggctaacatt attcagtatc atcgcattta tgttattaca ttcacaatca
     1081 tttgttgtaa tagtaatcgg tatatttata ttaggatttt tcttatcaac ttacgaagcg
     1141 acaatgccag ggtcgttacc aacgatgttt tacagtcata taagatatcg aactttatca
     1201 gtaacattta atatctctgt ttcgatattt ggtggtacga cgccattagt tgcaacatgg
     1261 ttagttacga aaactggaga tccattagca cctgcgtatt atttaacagc aatcagtgtt
     1321 attggctttt tagttattac attcttacat ttaagtacag caggaaaatc tctaaaaggt
     1381 tcgtatccaa atgtagataa cgagcaagat agagcttatt atgcagaaca tccaaaagaa
     1441 gcattatggt gggttaaaga acgtaagaat tagagatttt aataaaaagt ataaatcaat
     1501 cgtatataag cactttaaag ctagtaggtt ctgctaactt taaagtgctt tttaaattga
     1561 gaactgtaat tagccgtaat aaagtttttg tatatacata aacccccact gcaatgatta
     1621 tcgcaatggg ggaaagaggg gacttaaagc atatgtttag ctttgaatac ttaaaattct
     1681 cttgctattg aaatgttagg atgtaaatat gtcttagagt attttgtcca acgcaattaa
     1741 tattgagact ctaaccttca atattattat agagaacaca aacttaaata gattgggtga
     1801 cttatttgtg tcagttattg cgattgcgat aacttctttt ctctatatac atatagtaac
     1861 gtcttatcta ataaaaaaca tggtactaca gtatcaaatt tatctagggc ttaagtttga
     1921 tttttataat aggcaggttt acctgataaa aatacttatt cattatataa tgttaacaat
     1981 atgtatttta aagtttacat tgagtgaggg atattgatga acgtaatttt agaacagttg
     2041 aaaacacata ctcaaaataa acctaatgac atagcattac atatcgatga tgaaacaatt
     2101 acatatagtc aactaaatgc ccgcatcact agcgcagttg aatctttgca gaaatattca
     2161 cttaaccctg tcgttgctat taatatgaaa tcaccggtgc aaagtattat ttgttattta
     2221 gctttgcatc gtttacataa agtgcctatg atgatggaag gtaaatggca aagtactata
     2281 catcgtcaat tgattgaaaa atatggtatt aaagatgtaa ttggagatac aggtctcatg
     2341 cagaatatag actcaccgat gtttattgat tcaacgcaat tacagcacta ccccaattta
     2401 ttacatattg gttttacttc agggacaact ggactgccaa aagcatatta tcgtgatgaa
     2461 gattcatggt tggcttcttt tgaagttaat gaaatgttga tgttaaaaaa tgaaaatgca
     2521 atagcagccc ctggaccact atcgcactcg ttaacattat atgcgttatt gtttgcttta
     2581 agttccggtc gtacttttat aggacagacc acttttcatc ctgaaaagtt acttaatcaa
     2641 tgtcataaaa tatcatcata caaagttgct atgtttcttg ttccaacgat gattaaatca
     2701 ttattgttag tttacaacaa tgaacataca atccaatcat tttttagcag tggagataag
     2761 ctgcattctt ctatttttaa aaagataaaa aatcaagcaa atgacataaa tttgattgaa
     2821 ttttttggta catcggaaac cagttttatc agctataact tgaatcagca agcaccagtt
     2881 gaatcagtag gtgtgctatt tccaaatgtg gaattgaaaa caacgaatca cgatcacaat
     2941 ggtataggaa ctatttgtat aaaaagtaat atgatgttta gtggctatgt aagtgaacaa
     3001 tgtataaata atgatgaatg gtttgttact aatgataatg gctatgtaaa agagcagtat
     3061 ttatatttaa cgggacgtca acaggatatg ttaattattg gtggtcaaaa tatatatcca
     3121 gcacatgttg aacgcctttt aacgcaatct tcgagcattg atgaagcaat tatcatcggt
     3181 attccaaatg agcgttttgg tcaaataggc gtattgcttt attctggtga tgtgacactt
     3241 acacataaaa atgtaaaaca atttttaaaa aagaaagtga aacgctatga aattccatcg
     3301 atgattcatc atgtagaaaa gatgtattac actgcaagtg gtaaaattgc tagagaaaaa
     3361 atgatgtcga tgtatttgag aggtgaatta taatatgaat caagcagtca tagttgcagc
     3421 taaacgaact gcatttggga aatatggtgg cactttaaaa catttagagc cagaacaatt
     3481 gcttaaacct ttattccaac attttaaaga gaagtatcca gaggtaatat ctaaaataga
     3541 tgatgtagtt ttaggtaatg ttgttgggaa tggtggcaat attgcaagaa aagcattgct
     3601 tgaagcgggg cttaaagatt caatacctgg cgtcacaatc gatcggcaat gtgggtctgg
     3661 acttgaaagt gttcaatatg catgtcgcat gatccaagcc ggagctggca aggtatatat
     3721 tgcaggtggt gttgaaagta caagtcgagc accttggaaa atcaaacgac cgcattctgt
     3781 gtacgaaaca gcattacctg agttttatga gcgtgcatca tttgcacctg aaatgagcga
     3841 cccatcaatg attcaaggtg ctgaaaatgt ggccaagatg tatgatgttt caagagaatt
     3901 acaagatgaa tttgcttatc gaagtcatca attgacagcg gaaaatgtaa agaatggaaa
     3961 tatttctcag gaaatattac ctataaccgt taaaggagaa atattcaaca ctgatgaaag
     4021 tctaaaatca catattccga aagataactt tggccgattt aagcccgtga tcaaaggtgg
     4081 gaccgttacc gctgcgaata gttgtatgaa aaatgatggt gcagttttat tgcttattat
     4141 ggaaaaagat atggcatacg aattaggttt cgagcatggt ttattattta aagatggtgt
     4201 tacggtaggt gttgattcta attttcctgg cattggtcca gtaccagcca tttccaactt
     4261 actaaaaaga aatcaattaa cgatagaaaa tattgaagtc attgaaatta acgaagcgtt
     4321 cagtgcacag gtagttgcct gccaacaagc tttaaatatt tcaaatacgc aattaaatat
     4381 atggggtggt gcattagcat caggtcatcc atacggtgca agcggtgccc aattagtgac
     4441 tcgattattt tatatgtttg acaaagagac tatgattgca tctatgggga tagggggagg
     4501 tctaggaaat gcagcattat ttactcgatt ctaaccagcg attaaatgtg tcattttcta
     4561 aggatagtgt ggctgcatat tatcagtgtt ttaaccaacc ttatagaaaa gaagtaccac
     4621 cattaatgtg tgcgtcatta tggccaaaat ttgatttatt taaaaaatat gcaaatagcg
     4681 aactgatttt aacaaaatca gcaattaatc aaactcaaaa gatagaagta gacacaatat
     4741 atgtagggca tttagaagat attgaatgcc gacagactcg caatatcaca cgttatacaa
     4801 tggctttaac attaactaaa aatgatcaac atgtcataac ggttacacaa acttttatta
     4861 aggcgatgaa gtagagatga agtttaatga gatatggata aatgaatatt tggcgctcgt
     4921 aaatgatgat aatccaatac ataatgagat tgtgccagga caattagtga gtcaaatgat
     4981 gctgatggct atgtcattag agacaaacca gtgtcaaatt aactacgtta aacctatttt
     5041 aataaatgaa aatatcgaat tcattgaaca acacgaacac gaaattatag caattaatga
     5101 cgatggagag attaaaataa aaatttcttt gagcacaaaa aaataaccga tattagctgc
     5161 atgaacgcat attaattagg agatgaaagg acagctaata tcagttatgt attgttatta
     5221 ttattgggaa cagagatgaa tataggttac gtttctttct ttgcacgggg atgcattaat
     5281 ctaaaataat aataacaact atatcaatgt ttaataaatt ctggattatt ggaacgatta
     5341 gtcaatttaa ctaactttca tatgatctat atcgtcttgt aataaagaga gcaatttgaa
     5401 tatttcagta tcactaaatg aatcgtcaca tttaattgaa acatgctgaa acgttttggt
     5461 tataatttca taaactggtg cgccttcatg gtgatactgt cgataaataa tcataaccta
     5521 tattacctcc tttgctactc tatggttata ttataaataa catttttatg tgtgacatca
     5581 accttaagta tcaacttttt atcagacata gaacgtatga tttactaaga ctatttatgt
     5641 ataaaagttc taaataaata
//
LOCUS       DBNENW010000151         5654 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNENW010000151 DBNENW010000000
VERSION     DBNENW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.153"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            114..830
                     /locus_tag="AC1LW8_002284"
     CDS             114..830
                     /locus_tag="AC1LW8_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP4774079.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            830..1303
                     /locus_tag="AC1LW8_002285"
     CDS             830..1303
                     /locus_tag="AC1LW8_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIP4774080.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEFSNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1395..1511
                     /locus_tag="AC1LW8_002286"
     CDS             1395..1511
                     /locus_tag="AC1LW8_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774081.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1656..2300
                     /locus_tag="AC1LW8_002287"
     CDS             1656..2300
                     /locus_tag="AC1LW8_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HIP4774082.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2416..3282
                     /locus_tag="AC1LW8_002288"
     CDS             2416..3282
                     /locus_tag="AC1LW8_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4774083.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3483..4703
                     /locus_tag="AC1LW8_002289"
     CDS             3483..4703
                     /locus_tag="AC1LW8_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HIP4774084.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4700..5188
                     /locus_tag="AC1LW8_002290"
     CDS             4700..5188
                     /locus_tag="AC1LW8_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIP4774085.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5287..>5654)
                     /locus_tag="AC1LW8_002291"
     CDS             complement(5287..>5654)
                     /locus_tag="AC1LW8_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HIP4774086.1"
                     /translation="FLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTI
                     QLQCFNNSNWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVM
                     PIGLRIERNISKKHSHTFL"
BASE COUNT         1990 a          743 c         1031 g         1890 t
ORIGIN      
        1 cagtttgaag gaatcgtagt tcaataatgt taattgtgaa aatgtatata aacataaaaa
       61 aatcatgtat aatatatgtt gttaattaaa cagttcgaaa gcgagatgac attatgggac
      121 gtaaatggaa taacattaaa gaaaaaaagg cccaaaaaga taaaaacaca agtagaatat
      181 atgcgaaatt tggtaaggag atttatgttg cagcaaaatc tggtgaaccc aatccagaat
      241 ctaaccaagc tttaaggttg gtgcttgaac gcgctaagac atattcagtg ccgaatcata
      301 ttattgaaaa agcaatagat aaagctaagg gtgctggaga cgaaaacttt gatcacctaa
      361 gatatgaagg atttggccca agcggatcaa tgctaattgt tgatgcgtta acaaataatg
      421 taaatcgtac tgcctctgat gtgcgagctg cttttggtaa aaatggcggt aatatgggtg
      481 tatctggatc agttgcttat atgtttgatc atgtggcaac atttggtatt gaaggaaagt
      541 ctgttgacga aatacttgaa acattaatgg aacaagatgt agatgtaaat gatgtgattg
      601 acgataatgg attgacaata gtctatgctg aaccagatca atttgcagtc gttcaagatg
      661 cgcttcgtgc agcaggtgtt gaagaattta aagttgctga atttgaaatg ttacctcaaa
      721 cagatattga actttctgaa gcggaccaag taacatttga aaaattaatc gatgcattag
      781 aagatttaga agatgtacaa aacgtattcc ataatgtgga tttgaaataa tgaaatcagc
      841 agaacaatgg attgatgaat tgcaacttga atcacatcct gaaggtggtt tctatagaga
      901 gacaattcga gaagtattga aagatggacg cagagcgccg tttagtagta tttatttttt
      961 acttacagat gacaatattt cgcattttca tcgaattgat gctgatgaag tatggtacta
     1021 tcatgctggc gattctctaa caattcatat gataaatccg gatggggaat atacgactgc
     1081 aacattgggt actgatatcc aaaatggaga tgtattgcaa tatgtagtgc ctaaaggaac
     1141 aatttttgct tcttcaatcg aattttcaaa tacttatagt ttagtaggtt gtatgtgtca
     1201 accggcattt gagtttaagc agtttgaatt gtttaagcaa tctgaattaa ttacacaata
     1261 tccgcatctt aaatcagtga ttgaaaaata tgctttaaaa taaaaatgat caatgaagtg
     1321 gtttgaaggt tgttaataaa cctttgagtc acttcatttt tgtatgtatt cttgattgaa
     1381 tcagaataga attgatgcta cagctgtttt taatgaaaat agcattaaat gattttgaaa
     1441 acgataagag tgtgttattt atatttttga aaaatcactt ttatgaagaa atgtgtgtac
     1501 tatctaatta atattgtcta ttttaagtaa attgaagcga gttgtagtaa aataataata
     1561 aatattttca tatgattaac aaaaactata aaactgtatc atgacatgac atcatttatg
     1621 tagttttata taataatgac aaggagttgg tgactgtgaa agtaaagtat atagataaac
     1681 gtcactggcg tcgcctaatt gatagggaat acacagaggt aaaagttaat aataataggt
     1741 ttaagggtat tataggctta gtcacgatga aaaaggttcg tgatccttta gaggtgacgg
     1801 tagttggaca aaatatcatt gtcgcagatg acaattataa atggttgcaa atactacctg
     1861 aaaagaaacg ttatagtata actgtaatgt ttgataataa aggcaatcca ttagaatatt
     1921 attttgatat aaatatcaaa aatataacgc aaaaaggtaa tgcgcgtaca gtagatttat
     1981 gtttagatgt tttagcgtta ccaagtggtg aatatgagtt ggtagatgaa gatgacttaa
     2041 tgtttgcatt agaaagtgag caaattacaa aaaagcaatt tcatgaagca tatatgattg
     2101 cacatcaaat tatggcagag ttagaaaatg attttaaagg attccaaaag aaaatcatgt
     2161 actgctttaa taaaattaat gcaaaggctc aaaaaaatca tcaaaagcca caaaataaaa
     2221 ctaatattga aaaaagcaaa caaataaagc ctaagcaata taatcaaact aaaaatcacc
     2281 aacaacaaaa gaaaaactaa gtaattcaag ctgcagccat gccaataaaa ttggtgaaat
     2341 tattcgttag taatgaactc ttgaatgctc gtagtcatga aagttcaata attgaataat
     2401 ttatggggga aaattatgaa gattgaagac tatcgtttac taataacatt agacgaaacg
     2461 aaaacgttac gtaaagcggc tgaaatttta tatatatctc aacctgctgt tacacaaaga
     2521 ctaaaagcta ttgaaaatgc ttttggagta gatattttta tcagaacaaa aaaacaattg
     2581 attacaacaa ctgaaggaac aatgattatt gagcatgccc gtgacatgtt gaaaagagag
     2641 cgattatttt ttgacaaaat gcaggcacat attggtgaag tgaatggaac aatatcaatc
     2701 gggtgttctt ctttgattgg acaaacctta cttcctgaag ttttgagcct atataatgcc
     2761 caatttccta atgttgaaat acaagtgcaa gttggttcaa ctgaacaaat taaagcaaat
     2821 catagagatt atcatgttat gataactcgt ggaaataaag taatgaattt agctaacaca
     2881 catttattta atgatgatca ttattttatt tttccaaaaa atagacgaga tgatgttaca
     2941 aagttaccat ttatagagtt tcaagctgat ccgatttata taaatcaaat aaaacaatgg
     3001 tataacgata atttagaaca agattaccat gcaactatta cagtggatca agtagcaact
     3061 tgcaaagaaa tgttgattag tggtgtaggt gttacaattt tgccggaaat tatgatgaaa
     3121 aatatcagca aagaacaatt tgagtttgaa aaagtagaaa ttgataatga accgctgatt
     3181 cgttcgacat ttatgagtta tgatccgagc atgttgcaat tgccacaagt tgattctttt
     3241 gtaaatctca tggcgagctt tgttgaacaa ccaaaggcgt agttttagac taatttaagg
     3301 tttgtattta gttttaaact attcggttaa attgaacgta gttggttgct aatgcaccaa
     3361 cagcaaaaga gcccctaatt aataaattaa aaggggacaa aggaatacag ttggttgcta
     3421 atgcaccaac tgcataagag cccctaatta ataaattaaa aggggctcta agaagcgggg
     3481 ttatggatat gtttgcagcg ttattacaaa taaagaatta taaactcttt gttgctaata
     3541 tgtttctact aggtatgggt attgcggtta cggtcccata tcttgttctt tttgcaacta
     3601 aagatttagg tatgacaaca aatcagtatg gattacttct agcatctgca gcgattagcc
     3661 agtttacagt aaattcaatt attgctagat tttcggatac gcatcacttt aatagaaaaa
     3721 ttattattat tctcgcatta ttaatgggtg cgcttggttt ttcaatatac ttttttgtag
     3781 atacaatctg gttattcata ttactatatg cgattttcca aggattattt gcaccagcaa
     3841 tgccccaact ttacgcatct gctagagaat ctatcaatgt ttcaagctct aaagatagag
     3901 ctcaatttgc caacacagta ttacgttcaa tgttctcatt gggcttttta tttggtccat
     3961 ttattggtgc ccaattaatc ggtttaaaag gctatgctgg attgtttggt ggaacaataa
     4021 gtatcatttt atttacttta gtacttcaag tgtttttcta taaggattta aacattaaac
     4081 accctattag tacgcaacaa catgttgaaa aaattgctcc taatatgttt aaagacaaaa
     4141 cgcttttatt accatttatt gcatttattt tattacacat tggacaatgg atgtatacga
     4201 tgaatatgcc tttatttgtt actgattatt taaaagaaaa tgaacaacat gtcggttatt
     4261 tagctagttt atgtgctggt ttagaagtgc catttatgat cattcttggc gttttatcat
     4321 ctagattaca gactcgaaca ttgttgattt atggagcgat ttttggtggt ttattctact
     4381 tcagcattgg ggtatttaaa aacttctata tgatgttagc aggacaggtg tttttagcta
     4441 ttttcttagc ggttctttta ggaattggta ttagttattt ccaagatatc ttaccagatt
     4501 ttccaggata cgcctcaaca ctattttcta atgcaatggt tattggacag ttaggcggta
     4561 acctattagg tggtgctatg agtcactggg taggtttgga aaatgtattt tttgtatcag
     4621 cagcatcaat catgttaggt atgatactta tattctttac taaaaatcaa aaaattacaa
     4681 aagaggatgt gatatcaaca tgacaattat tttatggcta cttatcatcg ctgccttcat
     4741 gttagcattt gttgggttga ttaagccgat tattccttct gttttagtat tatgggttgg
     4801 ctttttaatc tatcaatttg gctttcataa tcagcattta tcatgggtgt tttatgtatc
     4861 tatggcattg ctaacaatat taattttatg tgccgacttt ttagctaata aatattttgt
     4921 gaatcgcttc ggtggttcta agtttggaga gtatgcagct ttaattggtg tggttattgg
     4981 atgttttgtt ttaccgccat ttggaattat tattatacct tttattttgg tattcatagt
     5041 tgaattaata caaggctatt catttgaaag agcagttaaa gtaagtatag gttcaatcgt
     5101 agcattttta acaagtagta tagctcaagc aatcattatg tttataatga ttgtatggtt
     5161 ctttatagat gctttattga ttaattaata aaaagcttat tgcaaaatat gtttttcggt
     5221 aactgtaatt tagtgatttt atcattaaca gtaccaaatt cgcatatgat gtaataagct
     5281 ttttgtttat aaaaatgtat gagaatgttt tttcgaaata tttctttcaa tgcgtaatcc
     5341 aataggcata actataaatg aaaatataat aaatacccaa ttgcttacct gaatatatgg
     5401 tccaaaggca agtaatgatt gtggaataaa gaatacatag aaaaatccta caattagtag
     5461 aatgattgct aaataggtga atatccatat ccaatttgaa ttgttaaaac actgcagttg
     5521 tattgtttta aaactaaacc atataaataa aaagacaata aagaatccaa cgactactga
     5581 aaacgggaat gaaacaaaat ataaattact tccatttttt tccatgaaaa atcctaaaaa
     5641 tcctttgaga aaac
//
LOCUS       DBNENW010000152         5532 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNENW010000152 DBNENW010000000
VERSION     DBNENW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.154"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            23..583
                     /locus_tag="AC1LW8_002292"
     CDS             23..583
                     /locus_tag="AC1LW8_002292"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIP4774087.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            636..1565
                     /locus_tag="AC1LW8_002293"
     CDS             636..1565
                     /locus_tag="AC1LW8_002293"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HIP4774088.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1760..1933)
                     /locus_tag="AC1LW8_002294"
     CDS             complement(1760..1933)
                     /locus_tag="AC1LW8_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774089.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            1985..3364
                     /locus_tag="AC1LW8_002295"
     CDS             1985..3364
                     /locus_tag="AC1LW8_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIP4774090.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(3484..4512)
                     /locus_tag="AC1LW8_002296"
     CDS             complement(3484..4512)
                     /locus_tag="AC1LW8_002296"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIP4774091.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            4782..5183
                     /locus_tag="AC1LW8_002297"
     CDS             4782..5183
                     /locus_tag="AC1LW8_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIP4774092.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(5201..5409)
                     /locus_tag="AC1LW8_002298"
                     /pseudo
     CDS             complement(5201..5409)
                     /locus_tag="AC1LW8_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1883 a          915 c          868 g         1866 t
ORIGIN      
        1 tattatatag gagagatgtt gaatgacaca tcgcgcacta ttagttgttg actattcata
       61 tgactttatc gcagatgacg gcttactaac atgcggtaaa cctggacaaa atattgaaga
      121 ttttattgtt tctcgtatca atgactttaa ttattatcaa gaccatatat tctttttaat
      181 ggatttacat tatttacatg acattcatca tcctgaaagt aaattattcc caccacacaa
      241 tatcgtagat acaagtggta gagaattata cggtaaagta ggtaaattat acgaaacaat
      301 taaagcgcaa cctaatgtac atttcattga taaaacgcgc tatgattcgt tctttggtac
      361 cccgcttgat agtttattga gagaaagaag tattaatcaa gtcgaaatcg ttggtgtatg
      421 taccgatatt tgcgtgttac atacagcaat ttctgcatac aacttaggtt ataaaatttc
      481 agtacctgct gagggagtgg cttcatttaa tcaaaaaggg catgaatggg cacttgcaca
      541 tttcaaaaac tcattaggtg cagaggtaga acaacacgtt taaatcgtgc taaaataatt
      601 ataaagaata caatttacaa gggagatatt tgacaatggc taaaacatat attttcggac
      661 ataagaatcc agacactgat gcaatttcat ctgcgattat tatggcagaa tttgaacaac
      721 ttagaggtaa ttcaggagcc aaagcatacc gtttaggtga tgtgagtgca gaaactcaat
      781 tcgcgttaga tacatttaat gtacctgctc cggaattatt aacggatgat ttagatggtc
      841 aagatgttat cttagttgat cataacgaat tccaacaaag ttctgatacg attgcctctg
      901 ctacaattaa gcatgtaatt gatcatcaca gaattgcaaa tttcgaaact gctggtcctt
      961 tatgttatcg tgctgaacca gttggttgta cagctacaat tttatacaaa atgtttagag
     1021 aacgtggctt tgaaattaaa cctgaaattg ccggtttaat gttatcagca attatctcag
     1081 atagcttact tttcaaatca ccaacatgta cacaacaaga tgttaaagca gctgaagaat
     1141 taaaagatat tgctaaagtt gatattcaaa agtacggctt agatatgtta aaagcaggtg
     1201 cttcaacaac tgataaatca gttgaattct tattaaacat ggatgctaaa tcatttacta
     1261 tgggtgacta tgtgactcgt attgcacaag ttaatgctgt tgaccttgac gaagtgttaa
     1321 atcgtaaaga agatttagaa aaagaaatgt tagctgtaag tgcacaagaa aaatatgact
     1381 tatttgtact tgttgttact gacatcatta atagtgattc taaaatttta gttgtaggtg
     1441 ctgaaaaaga taaagttggc gaagcattca atgttcaatt agaagatgac atggccttct
     1501 tatctggtgt cgtttctcga aaaaaacaaa tcgtacctca aatcactgaa gcattaacaa
     1561 aataatacta tattactgtc taattataga catgttgtat ttaactaaca gttcattaaa
     1621 gtagaattta tttcactttc aatgaactgt tttttattta cgtttgacta ttttacaacc
     1681 ccttttcaat agtagttttt attcctttag ctaccctaac ccacagatta gtgatttcta
     1741 tacaattccc cttttgtctt tacattttct taaaatattt gcgatgttga gtataaattt
     1801 ttgttttctt cctacctttt tcgttatgat taaagttata aatattatta tgtacacgat
     1861 tcatcgctct attttcaact ttcaacatat ataattcgaa agaccattta aaattaacgg
     1921 ccacaacatt caaatcaatt aatcgctttt tccaaaataa tcatataagg aggttctttt
     1981 cattatgaat atcattgagc aaaaatttta tgacagtaaa gcttttttca atacacaaca
     2041 aactaaagat attagtttta gaaaagagca attaaagaag ttaagcaaag ctattaaatc
     2101 atacgagagc gatattttag aagcactata tacagattta ggaaaaaata aagtcgaagc
     2161 ttatgctact gaaattggca taactttgaa aagtatcaaa attgcccgta aggaacttaa
     2221 aaactggact aaaacaaaaa atgtagacac acctttatat ttatttccaa caaaaagcta
     2281 tatcaaaaaa gaaccttatg gaacagtttt gatcattgca ccatttaact atccttttca
     2341 actagtgttc gaacctttaa tcggtgctat tgcagcaggt aatacggcaa ttattaaacc
     2401 atctgagttg acaccaaatg ttgcacgagt gattaaacga ttaatcaatg aaacatttga
     2461 tgcaaattac attgaagtta ttgagggagg aattgaagaa acgcaaacgt taattcactt
     2521 accttttgac tatgtcttct ttacaggaag tgaaaatgta ggcaaaatcg tttatcaagc
     2581 tgccagcgaa aatttagttc ctgtgacatt agaaatgggt ggtaaatctc cagtcatcgt
     2641 tgatgaaaca gcgaatatta aagttgctag tgagcgcatt tgttttggga aattcactaa
     2701 tgccggccaa acatgtgttg caccagatta cattttagta cacgaatctg taaaagatga
     2761 tttaatcaca gccctatcaa aaacgttgcg tgaattttat ggtcaaaata tacaacaaag
     2821 tccagattat ggccgcattg taaaccttaa acattatcat cgtctgactt cattacttaa
     2881 cagtgcacaa atgaatattg tatttggtgg tcatagtgat gaggatgaac gttatataga
     2941 accaacattg ttagatcacg ttacaagtga ttcagcaatt atgcaagaag aaatttttgg
     3001 tcctatctta ccgattttaa cgtatcagtc attggatgaa gcaatagcct ttattcacca
     3061 aagaccaaaa cctttgagtt tatatttatt tagcgaagat gaaaatgcta cacaacgtgt
     3121 aataaacgag ctatcatttg gcggcggcgc tattaatgat acattgatgc acctagcgaa
     3181 tcctaaatta ccatttggtg gtgttggcgc ctcaggtatg ggacgctatc atggtaaata
     3241 ttcattcgac acttttacac atgaaaaaag ctacattttc aaatctacac gattagaatc
     3301 aggtgtccat ttaccaccat ataaaggtaa atttaaatac atcaaagctt tctttaaaaa
     3361 ttaatttttg agccaaaaat aaaccatgct tccataattt gatgttcttg aaaacaataa
     3421 atttcaaggt atacacaaat tttttggaaa catggttttc tttttatcat gattaattaa
     3481 agattaaaat atgacacata caccttcaga tgctacacga gtgttatcac atagcgtaat
     3541 tttacctgtt tctttatttc tttcgaaaac agttacaact gaatctcctt gctcatgagc
     3601 acaaactaaa aggtcatctg atgaggcaat attaaaatct cttgggaatt gaccaccact
     3661 ttcagtaatt gttactagtt ctaagtgttg accattatca agaactttaa aaattgcaat
     3721 gctatcatgc cctctattag atacatataa gaattgttga tcatgagata aacgcactgc
     3781 tgcaagttta gtatctccat caaagttttc aggaattgtt aaatgacgct cgagctcttc
     3841 aaatttaccg tcattatatt ctgcaacact cacagtattt gataattcgt gtacgacata
     3901 agcaaatttt ccattatcat gaaattcaat atgtcttgtc ccatcactat ctttaaataa
     3961 agattcttta taaaattcaa acccgttgtc atcaaattta taagtaacga tacgatcagc
     4021 acctaaatct gttactgcaa cgtacttacc atctggagtt tgattaatat aatgtgcgtg
     4081 tggatgatct tgtctttcat gtgtacctgt tggaaaatca tgcgcaagtt cttgaattag
     4141 acgtataatt tcacctgtgt gcgtgtttaa ttcatacata cgtatgatgc cagcaccata
     4201 taccgcttca aataaatatc gtttatcttc tgaaatcgat acataacaac ctgtaccagc
     4261 ttttgaagac aaacatttat ttaataaatg caattcacca ttgtcatcaa ttttcaaact
     4321 cgcaacaccg cattgttctc cttctttgtt gattccatat aaaacttcat tattacgcac
     4381 caaatatgta gacgcttcta attcaaatcc tgtttctaat aaatcaatac gtgactgatt
     4441 ttcgtttaat tcaaagcgat aaatcccttt accattcttt ttagtgtaag aaccaatata
     4501 tccatttgtc attactttta cccctccatg ttagtcttat tttcatttta ttaaatttta
     4561 gctagaatta acaatcattt atcatttcct taaagctaaa actatttaac tactttaaaa
     4621 gaactatttg ctgtgatatg tcattattcg attttaatta atccctcttt tttacttaca
     4681 cgagcattaa ttattaacgc gtttatgctt ccacaaaatt tttgttgcaa aatagaacac
     4741 atgttcgtat aatgtaatta tcagattggg gtgaattaag gatgacaagt tatgaagaag
     4801 aaactgatta tcgtaacgtt ccaagagaat tactagataa caatatacct atgggaagag
     4861 gcatgattaa atgggctcct tttgcaacat tacccgaaca attcgaaacg attcaacaat
     4921 acattatcga ccaaaacaaa attacacgtc ctgtattatc agatgatcaa ttggctgagc
     4981 taaacattcg gctacacgag gcgctacaat atgcccaacc tgtagaagtt aaattttata
     5041 ataatggctt tgtagattca gtacgcttaa ccatttatcg tattgatgct attaattatg
     5101 aaattgatgg ttatgtttac aatcagcaac aacgtcaaaa aatttctatc tttgatatct
     5161 tagatattgt cttgttacct taatgctttg aacataatta aacagattga gtatatgcat
     5221 ataagatgat ttttaacatc atttttggat gataggatgt tgcgccacga tgatgtctga
     5281 attcatcgaa ttcgctatca ggtattgttt caacaatttc attaacatat cgtgaaatat
     5341 cattttgagg gattctaaca gaagtttcta ttggtagtgt agttgggtca tgttataatt
     5401 tttatacata aggcacctcg ttaatttagt ttagtggtat ttattaaatt ataagaaggg
     5461 acccaacaca gaaaattcat tttattgaat tttacattta tgtgcaagtt gggcaaagtg
     5521 tcttattttt ta
//
LOCUS       DBNENW010000153         5527 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNENW010000153 DBNENW010000000
VERSION     DBNENW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.155"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            4..813
                     /locus_tag="AC1LW8_002299"
     CDS             4..813
                     /locus_tag="AC1LW8_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP4774093.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            842..1045
                     /locus_tag="AC1LW8_002300"
     CDS             842..1045
                     /locus_tag="AC1LW8_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6501 family protein"
                     /protein_id="HIP4774094.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            1226..2017
                     /locus_tag="AC1LW8_002301"
     CDS             1226..2017
                     /locus_tag="AC1LW8_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP4774095.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            2031..3917
                     /locus_tag="AC1LW8_002302"
     CDS             2031..3917
                     /locus_tag="AC1LW8_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774096.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDIVVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            4142..5485
                     /gene="brnQ3"
                     /locus_tag="AC1LW8_002303"
     CDS             4142..5485
                     /gene="brnQ3"
                     /locus_tag="AC1LW8_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid-like transporter
                     carrier protein BrnQ3"
                     /protein_id="HIP4774097.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         1985 a          780 c          966 g         1796 t
ORIGIN      
        1 ataatgtgtg gacttagaag tataacatta ggtacaacaa atatagaaca gacaaaacat
       61 ttcatggttg acatattagg attaaattat gaagaacttc ttgaaaactc aattcgtttc
      121 ggcgatgcag atataagccc aggaacaaga cttcaattta tacaagttcc aagtgagcaa
      181 ttagaagaat ctcactttgt gggtattgga ttacgtacac caactgactc aggtttagag
      241 gagtatgcgg aaatattatc gaataaggat attccattta caacagttaa agaattaaat
      301 ggcaataaat atttcagtct cgaagataac aatggtcata ttttctcaat atattcaaac
      361 gagaataatt atggcgttgg tttaggtatg ccttcttttg agagtgcggt caatccgtta
      421 catcaagtgc aaggtttagg accagtgatt cttaaagtga atcatgtaga tattacaggt
      481 caaattttaa caaatatatt cggacttgaa gtatttgcag aataccaacc cttcgacaat
      541 gctgactatc atgtccaagt attcaaagtt ggaacgggtg gtctaggtgg cgaaatacat
      601 ttaatgcctg tagaaaccga aatgacaatg ccggaatatg gcgcagtcga tcaagttgag
      661 tttgaaacga aagatgcaga tttctttaat caagcgaaat cgcgcttaga tgaagtggaa
      721 ataccatatc aaacgcttga gcaagatgat attgaatcaa ttagaattac tgaaaacagt
      781 ggattatcgt ttatattcac tttacaaaaa taattttttt acgatagcga ggacaaaatt
      841 tatgttacat gaaacttgga aagaacgtac accaatcaag aaagtagaag tcattaatac
      901 agatgcaaag aaatttacag tttctgacat gttaacggtt ggcaaacaat atgatgttat
      961 caacgaaact gaagaatatt atcaaataat agataattct ggattagttg gcggttacta
     1021 taaaacatat ttcaaagaag tttaaacgca taattattga aatgaagcct aatatgagat
     1081 tttcaattag taaatttggt aaaatataaa caattcgcat actaagcatt gcgttttata
     1141 tcagaataga atagtaaact aaggcatttg gagaacttat tccatgtctt agttttttaa
     1201 tttatttaaa ggagaacgtt ttaaaatggc actaaaacat tataagaatt cagattcaac
     1261 agttttcaat gatgcgaagg cattatttga tttaaataaa aatattttac ttaaaggtcc
     1321 aacaggttca gggaaaacaa agttggcaga aacattaagt gaagttgttg atacacccat
     1381 gcatcaagtc aattgttctg ttgatttaga tacagaaagc ttattaggct ttaaaacaat
     1441 taaaacaaat gcggaaggtc aacaagaaat tgtctttgta gatggtccag ttattaaagc
     1501 tatgaaagag gggcatattt tatatattga tgaaataaat atggctaaac ctgaaacatt
     1561 gcctgtatta aatggggtct tagattatcg tcgtcaaatt acgaatccat acactggtga
     1621 agtaatcaaa gctgtaccag gatttaacgt tatagcagcg ataaatgaag gttatgttgg
     1681 tactttgcca atgaatgaag cactaaaaaa tcgctttgtt gttattcacg ttgattatat
     1741 tgatggggac attttaaaaa atgtgattaa agagcaaagt ttattacaag atgataaaca
     1801 aatcgaacaa attattaagt ttaatgaaga tttacgtact atgtctaagc agggacaaat
     1861 ttctgaagaa gccgctagta tccgtgcatt attagacttg tgtgatttaa tcactgtaat
     1921 gccagttgaa cgtgcaatta aacgtacaat tattgataaa ttggaagatg aacgtgaaca
     1981 acaagcaata tataatgctg tagaactaaa cttttaaaga gggataaaac atgagtgatc
     2041 gtttcataaa atttaatgac gaacagttag atgccaagca agtaatgatg ttgcaagact
     2101 tagctcgact cttattaaaa aatgaacaaa cacaagttaa aatccaaaaa ttcccttact
     2161 ataatcctgt tcaaaatgtt ttgattacga gttggttttg gtcacatcga ccaagccata
     2221 tcgaaatggc tggtttaaaa acagatgtta tgttagctgc ttacggttat catatgatgg
     2281 atgtacaaat tgtaaatgaa gtagtacaag ataaaacttt taaacaccct aaattttatc
     2341 aacaactttt taaattatta gaagatatgc gcgtattaaa ttctattaaa gttgagcgcc
     2401 caagtactgc taaattaatt gatcttcgct tagatacacg tatatcatat acggaatctc
     2461 aaattaaagt gtatcgtact aaaacacaat atacagattt attatttttg tatttagaac
     2521 atgcattttt aagccaagat ttctttgata taccatctat acattcagat ttagatgata
     2581 ttgtagttaa tatgttctta tatttaccta attttttcca aaatcaaaat tctgaagata
     2641 atatgtatct ggctcaaaga attatgtatc aagtagatga tattttaaaa gaagatatgc
     2701 taaacgaata ttattactta cctaaaacac tatataatac attagctagt ccagagtttg
     2761 atgatttaaa gagaactgat gcaagtcaag tcgatgggca agatgatact tcagaagatg
     2821 atgataacga aagtgaaaaa gctgactcta aatctgcaga tagtgagtca aaaggtggcg
     2881 cctatttaga aatggaactt cacgaaggac aaaatagtga aacattgggt aatgatgaag
     2941 cacgtgaagg cgatgctaca gatgatatga ctgatatgat gaccaaaaag ggcaaaggtt
     3001 caaacgatac gctaaatcgt gaagaaggcg atgctgttgg tcaaagccaa gcatttcaac
     3061 ttgatggtgt gaacaaaaat gtagaaataa aatggcaaat tcctgaaatt gagccacaat
     3121 atgttttaga atatcaagaa tcgaaacaag acgtacaata tgaaatcaaa gacttaattc
     3181 aaattataaa gaaaacgatt gaacgagaac aacgagatgc acgttttaat ttaacaaaag
     3241 gacgattaca aaaggattta attaattggt ttatcgacga tcaatataaa ttgttttaca
     3301 aaaaacaaga tttaagtaaa tcatttgatg cgacgtttac attgttaatt gatgcgtcag
     3361 caagtatgca tgataaaatg gctgaaacga agaaaggtgt agtgttattc cacgagacgt
     3421 taaaagcatt gaacattaaa catgaaattt tatcattcag tgaggatgcc tttgattcag
     3481 acgatcatgc acaaccaaat atcattaatg aaattattaa ctatgattac tcaacctttg
     3541 aaaaagatgg cccacgtatt atggcacttg aacctcaaga tgataatcgt gatggtgttg
     3601 ctattagagt tgcaagtgaa cgattaatgc gtcgaaatca acatcaacga tttttaatcg
     3661 tattttcaga cggcgaaccg tctgcattta attatagtca agatggtatt attgatacgt
     3721 atgaagctgt agaaatgtca cgtaaatttg gtattgaagt atttaatgta tttttgagtc
     3781 aagatccaat aactgaagat gttgaacaaa caattcataa tatttatggt caatatgcta
     3841 ttttcgttga aggtgttgcg catttacctg gacatctctc tccattatta aaaaaattac
     3901 tacttaaatc tttataaata agtttaaaat atgagtttat atgaaactgt gaataaaagt
     3961 catcagttac tatttaaaaa gtaaatttga agaattttat tttctggcat aatttgattt
     4021 aaacaacaca tgtgttttgt ttctgctatg tacaacacat tccatataga ctctttatta
     4081 ttttttgtgc taaaatattt acttgtaaat tatttcgtgt tttaaaaaaa ggagtaactc
     4141 tatgaataaa aatacatggg tcattgggtt tacgctcttc gctatgtttt ttggcgcagg
     4201 caatctaatt tttcctccta atctcggatt ggatagtggt caatttttct ggcccgccat
     4261 tttagcgttt gttctaactg ggattggttt accattatta ggtgtgattg taggtgcact
     4321 tgataaagaa ggatatattg gcgcattaaa taaaatttca cctaaatttt caatattgtt
     4381 cttaatcatc atttatttga ctataggacc acttttcgca atacctagaa ctgcatctac
     4441 atcttttgaa atgacaatta caccaattat acatagcaat agtagtatcg ctttatttat
     4501 atttacgatt atctacttca tagtcgtttt gtatatttgt ttaaatccat ctaagttaat
     4561 cgatcgtatt ggttcattat taacaccatt attattgatt actattttag cgatgattat
     4621 taaaggatac ttagacttta gcggtaatag tgctggaaag ggcaatgaag cactatatca
     4681 ttctaatttt tcaagttttg ctgaaggctt tacacaaggc tatttaacaa tggatgccat
     4741 tgcagcaatt gctttttcaa tgattgttgt taatgcagta aaactaacag gcattactaa
     4801 aacaaatcaa atattcaaac aaactttgac tgctggttta attgcagccg tagctttaat
     4861 tttcatatat atttcattag gttatattgg taatcatatg ccagtaagtg acatgacgtt
     4921 agatcaattg aaatccaaag atcgaaacat tgggacatat ttattaacga caatggcttc
     4981 aacaggattt ggttcattcg gaaaatattt attgggcatc attgtggctc tggcatgtct
     5041 aactacagca tgcgggctta ttgttgcagt ttctgaatat ttccatagaa tcgtacctaa
     5101 agtatcatac aaagcatttg tattagtttt cattttaatg agttttatta ttgctaacca
     5161 aggtttaaat gctgttatct caatgtcaat tccggtatta agcattgtat acccagtagc
     5221 aataactgtt gtattattaa ttttaattgc caaattcata ccgacaaaac gcatttcaca
     5281 acaaattcca gttattatcg tatttatatt gtcgattttc agtgttatta gtaagttagg
     5341 ttggctgaaa attaacttta tagaatcatt gcctctaaga gcgtattctt tagagtggtt
     5401 cccagtagca attattgcaa cgatattagg ctatctagtc ggcatatttg taaaacaaga
     5461 tccaattaaa tatcaacagg aataacgaat aatataaaag aggttgggac ataaatccct
     5521 aaaaaaa
//
LOCUS       DBNENW010000154         5499 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.156, whole genome shotgun sequence.
ACCESSION   DBNENW010000154 DBNENW010000000
VERSION     DBNENW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.156"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(49..1611)
                     /locus_tag="AC1LW8_002304"
     CDS             complement(49..1611)
                     /locus_tag="AC1LW8_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIP4774098.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            complement(1919..2926)
                     /locus_tag="AC1LW8_002305"
     CDS             complement(1919..2926)
                     /locus_tag="AC1LW8_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIP4774099.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            complement(3160..4161)
                     /locus_tag="AC1LW8_002306"
     CDS             complement(3160..4161)
                     /locus_tag="AC1LW8_002306"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIP4774100.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEETDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTGTLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            complement(4444..5313)
                     /locus_tag="AC1LW8_002307"
     CDS             complement(4444..5313)
                     /locus_tag="AC1LW8_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP4774101.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
BASE COUNT         1748 a         1073 c          789 g         1889 t
ORIGIN      
        1 atttagtaat atattcccta gtttttgtaa ataaaacctc attattaatt aattttcatc
       61 aatatgtttt gaagaacggt attctaaaat atctgggtca cgatgtttaa ttaaaacctt
      121 attactattt ctcggtttct cctcactcaa agattttaaa agcgaccata tcatcgctat
      181 aatgaccacg gaaaatggta acgcagcaat gattaataaa ttttgtatcg cttgcgtacc
      241 acctgtgtaa atcataataa ttgcaaataa tgccataatg ataccccaac tcactttgac
      301 aaatgacttc ggattaatat cacctcttga actcaacata cctaaaacat aagttgccga
      361 atccgctgat gtaacaaaga aaatcataat aacaagtaaa gtaattaagc ttaatacaaa
      421 acctagtggg taatgttgta gcgttgcaaa agttgctgtt tctgtcgctg ctttagcaat
      481 atcggcaata tgattatctt gtaagtaaat tgctgacgcg ccgaataccg caaagaatat
      541 aaagcaaact aatgccggga caaaaagtac acctagaata aattctttaa tcgtacgtcc
      601 ttttgacaca cgtgcaataa atatacctac aaatggtgcc caagatatcc accatgccca
      661 gtaaaagatt gtccaatttt gtaaccattg gaatttttga ccgcctgacg gaatgcgtaa
      721 actcatacta aagaaatttg caatataatt acctagacca ttcgtaaatg tatttaaaat
      781 gtatagcgtt ggcccaacaa taaaaagacc aataagtact acaaaagcaa gtaacatgtt
      841 gatattactc aacgttttga tacctttatc tatacctgac catgctgacc aagtaaataa
      901 tatggttgca atgacaatca agattacttg catcgtgaaa ttactcggta cattaaataa
      961 aaaatgtaaa ccttcgttta tttgcaatgc accgaaacct aatgttgcag caacgcctgt
     1021 tactgtagct atgactgcca atacatcaat ggcaccacct aaaggtccac gcattgcctt
     1081 ttcacctaat aaaggcgtca atgtagcgct gactaagcca ggatatcctt tatgaaagct
     1141 aaaatatgca aacacaagcg cgacaatacc atagactgcc catgcatgaa tcccccaatg
     1201 gaaaaatgaa aactgcattg catcattaat tgcagattgc gtgccagctt tatgaatagg
     1261 cgttaatttg aaggcatgac tgattggttc tgccgttgtc cagaacacaa gtcctattcc
     1321 cataccagca ctaaataaca tagcaaacca agacggcaat gagaattcag gatcttcgcc
     1381 ttcttcacct aatgtaatgt ttgcgtatct cgaaaataaa atatacacac agacaaataa
     1441 aataactaaa acgagcaata aataatacca agaaaaatgt agcgcaataa atgtagtaat
     1501 gttttgcgtg agtttttcta actgtttcgg aaatattgct ccaaaagcaa caaatatcgt
     1561 acatatcact aaagataccc aaaacactag acttactgat ttatttttca taaatacaaa
     1621 ccctttctgt gtaatggtaa gttcataccc ataactgcaa cattttaatc atttataatt
     1681 ttatatagac acaattaata atgcctcatc ttttaaaaat gatatataaa acacactcaa
     1741 attatttatc attgagcaac aaagtatttt attgtattta agtaatgcct ttctaatgca
     1801 ttattgaatt gatacctgca aagttgccat atttccattt agaatcaata gtcgctagac
     1861 acaaaaaata agtcgcccta tacagtattt tctgcataag gcgactttac ttactaaact
     1921 atatattaat tactaatttt ccaatcattg attgtttttc caacaattga tgtgcttgat
     1981 ataagttttc aggtgataaa ccttcaaaaa cttgtgtcgt tgttggttgg taatgccctg
     2041 attctatatt tttcgtaata tcttctaaat actcatgttg tttaatcata tcaggcgttc
     2101 gatgaattgg acgcgcaaac ataaattcat gtgtaaatgt tatacttttt aattttagtg
     2161 catttaaatc ttgatcttca ttaaaagcta cgatagttgt aatatgccct aatggtttta
     2221 tcagttcaat catagtattg taatacaagt ctgtattata ggtgcaaaat atataatcta
     2281 ctaatggaat ttctttttct ttaaattgac gcactaaatc ctctttatga ttcaatacga
     2341 tatctgcgcc catcttttca caccactctg ttgtttcttg tcgtgatgct gttgtaatga
     2401 cagttaaacc ataccgttta gcaatttgag tggctatact gcctacacca ccggcaccat
     2461 taatgattaa gacagacttc ccttcgtttt cagcaggatt atgtgaaatt ttaaatgtat
     2521 caaaaaacgt ttcatatgcc gtaataccag ttagcggtag actaaccgct tcattagcac
     2581 ttatgttgtg tggtgctttt gcaactatag cttctgacac caattgatat gtcgcatttg
     2641 atccttgtct atttggcgat ccagcataaa atacaacgtc acccggacta aataatgtaa
     2701 cgtctggtcc gatagcttca acagtaccaa tagcatcaaa cccaagtaca cgaggtgctt
     2761 gagtgacttc catttgtcgt tgctttgtat ctacaggatt tacactaatg ctatttactt
     2821 tgactaagat atcatcattt tctggcgttg gctttctttg ttcgtatact ttaaataaat
     2881 ttccttcttc caatttaaag ggcttttcaa atcctatcat tttcatatcg tttcacctca
     2941 tttatgaact tatttcttat tatacaaaat agaagccatg tgtgcttata tcgcagcatc
     3001 atgactcctt tttcatttga atatataaat aattacagac gactttcgta ttacatttta
     3061 gacttatttc taccatgtta ctgaacaaat ttgctttaga taaaaaatta ttaaatttta
     3121 gtcaattaac aaagttagtt tgttaaaacg tatactttat tattccgtta ctttaataac
     3181 ttgtttacca aagttatcgc cagtaaataa atttttaaat gcatgtggcg cattttcaaa
     3241 accatcttca atggttactt gtgactgaat tttaccttct tgaacccatg ttgcaagctg
     3301 ttcactagct tctttaaaag cattagcgaa ttcacttacc aagaagcctc tcatcattac
     3361 ttgcttctta ataagcgtac cttgaatacg tggtccgata tctgcttcag gatgattata
     3421 tgacgaaatt gcgccacata ctggtacacg tgcaaaacga tttaaatgct taaatacttc
     3481 atcgccaact gttccaccaa cattttcaaa ataaacatca ataccatctg gtactgcttg
     3541 tgctaacgct tctgcaaaat cctctttctt ataatcaata ccagcgtcaa agcccagtgt
     3601 ccctgttaaa taatttactt ttttgtcgcc acccgcaata cctactacac ggcaaccttt
     3661 aatcttagca atttgaccta caactgaacc tacagcacca gatgcagctg aaaccacaac
     3721 agtatcaccg gctttaggtt gtccaatatc aagcagacca tgatatgctg tttgtcctgg
     3781 cattcctaaa acacttaaat ataaatcaag tggtacatct gtcgttggaa ctttagtaat
     3841 ttgatccgct tggacatgat taatgattcg ccaaggcaac atacctacaa cgacatctcc
     3901 ttttttataa tctgcgagtg tcgaatcaat tacttttgca acgacatggc taacaatcgg
     3961 tttaccaatt tcaaaaggct gtacatacga atctgcctta gtcatacgtc ctctcatata
     4021 tggatccact gaaatataca gcgtttgtac aagtacgcca tcgctctcaa gtttaggcgt
     4081 gtcagtctct tcaattttga atgtatcctc ttgaggcatg ccttctggta ttttgttaaa
     4141 aagaatttgt ttattttgca tcattaatca cctttcttta tttgaaactt ttacttattt
     4201 gttacttaag cgttaagttt gaattgtgtc ttcgtgatgt ctgtatgcaa atacattctt
     4261 agttgttata ttttgactta agcactgatt cattcatgta acttcaacca cattatattt
     4321 gctataatca taaatttaaa atgttacgac ttagacattt tatggaaact ctcaaacaat
     4381 agataatttt tgaaaagctc taatattaca agcttttttg cccccgaaaa actagcagtt
     4441 gctttatttt ttccataaaa agtcgattaa ctcattagca acattttcat tctcatgaag
     4501 ctgactatgt tgtgcaggct caccttcata tttagattct cgataacttt tcggactatt
     4561 tcccagtaaa tattttaatg atttcgaaga actattagac actctgccgt ctgaatgtgt
     4621 accatctttc aaatctccat aaatatttag cacttcgata cctttacctt tataaatatc
     4681 ttttagcacg cgtaattgct gataaggttg attcattcga cttggtttac catctttatc
     4741 aactgtaatt tcattgacat cttcattcat atttaaaaca ccattaaatg tccctgcaat
     4801 attcacttgt ttgtttaact gtggcagtga cttgtcgtta ccatatgtca tcatatattg
     4861 tgcaaatgtt aagttcccca ttgagtgacc gacaaagttg aatttatcga aattgtattc
     4921 agattgtaac ttagtcagta catttttaaa ccacgcagca ttcttatcca aatagccttg
     4981 tctgttattt tcaagttcaa ttttcacaat aggattcact gcatcttttc ttagtttccc
     5041 tttaaatgtc actgcaccat cctttgaaac gtaagcagtg atgatatctt tagttacccc
     5101 tcttttttct gcttgcttca ccataaactt ttcagaattg gcactaccac caaatccatg
     5161 taagaacaat gttggaattg gctttttaac aaattgctgt tgttgtattt taaatgtttg
     5221 tgcctgtcgt tgactaaaca ccaccgtaat aatagagcct ataataatag cgaccgctaa
     5281 caatgtcgta ataattacaa aaattttctt cacactttta actcccattc atgtctttta
     5341 tataagtata aaggatgtga ttaaaaatgt cctttagttg attttgaata catcattaac
     5401 ttttaagatg actttggaaa gttgtccgtt aacatttgtt aattgattgc ttctttagct
     5461 ttcaatggtg tgtcacccat tgattaatat ataaatatg
//
LOCUS       DBNENW010000155         5408 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNENW010000155 DBNENW010000000
VERSION     DBNENW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.157"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            51..737
                     /locus_tag="AC1LW8_002308"
     CDS             51..737
                     /locus_tag="AC1LW8_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIP4774102.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            727..1386
                     /gene="nth"
                     /locus_tag="AC1LW8_002309"
     CDS             727..1386
                     /gene="nth"
                     /locus_tag="AC1LW8_002309"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIP4774103.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1391..1732
                     /locus_tag="AC1LW8_002310"
     CDS             1391..1732
                     /locus_tag="AC1LW8_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpoC family protein"
                     /protein_id="HIP4774104.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(2296..4479)
                     /locus_tag="AC1LW8_002311"
     CDS             complement(2296..4479)
                     /locus_tag="AC1LW8_002311"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIP4774105.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(4476..5102)
                     /gene="recU"
                     /locus_tag="AC1LW8_002312"
     CDS             complement(4476..5102)
                     /gene="recU"
                     /locus_tag="AC1LW8_002312"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIP4774106.1"
                     /translation="MNYPNGKPYRKNSAIDGGKKTAAFSNIEYGGRGMSLEKDIEHSN
                     TFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTPSTTDYNGVYQGYYIDFE
                     AKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKTLDEVYLLPYSKFEVFWK
                     RYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLILDESEDRV"
BASE COUNT         1827 a          848 c          902 g         1831 t
ORIGIN      
        1 aaatgattta tactattaag taatggttaa atttaaaagg gggcacgaac atggataaat
       61 atcaattaaa agcaagacct gtagtgatac gaagagaatt attagaccat tacagtgact
      121 taggtttaga tgagcaagat ttagtcattt tgcttaaatt gatttatgct tccgaaacat
      181 caaataaaca gccttcaatt gaattacttc agaagggctc aactatgcaa ccacgcgata
      241 ttacaatggt tattcaaaat ttgatacaac gtgaattgct agaattacaa gttcaaaaag
      301 atgaagaagg tagatttacc gaatatatga atctagatcc ttttttcgaa aaattaagtc
      361 atatattgaa gcaacaaagt atggaaacga aagagcaaaa tagtaaagaa aaatttaaac
      421 aattatttag agttttagag gatacatttg caaggccgtt atctccatat gaaatagaaa
      481 cgttaaatca atggattgat gttgataaac acgatagtgc aattatacaa gcggcattag
      541 acgaggcgaa tagtttgaat aaacttagct ttaaatatat ggatcgtatt ttacttaatt
      601 ggaagaaaaa caatgtcaaa acaattgacg attctagaaa aataagagaa aaatttaata
      661 aaccgaaaat gacgcacaca gttaaaacgg tacctaaatt tgattggtta aacggggaga
      721 accttgatgg taagtaagaa aaaagcatta gaaatgattg atgttatagc aaatatgttt
      781 ccggatgcag aatgtgaatt aaaacatgat aatccgttcg aattaactat tgctgtatta
      841 ttgtcagcgc aatgtacaga cgttctagta aatagagtaa caactgagct gtttaaaaaa
      901 tacaaaacgc ctgaagatta tttggcagtc agtgatgaag aacttatgaa tgatattcgt
      961 tctatcggtc tgtatcgtaa caaagctaag aatattaaga aactttgtca atctttgatt
     1021 gatcaattta atggagaaat accacaaaca cataaggaat tagaaagttt agcaggtgta
     1081 ggacgtaaaa ctgctaatgt agtcatgagt gtagcatttg atgaaccttc attagctgtt
     1141 gatacgcatg tagaacgtgt ttctaaacgc ttaggtatta atcgttggaa agataatgtg
     1201 agacaggtag aagaccgttt atgttctgtc atacctagag atagatggaa taggagccat
     1261 catcaactca ttttctttgg aagataccac tgtttagccc gaaaacctaa atgcgatatt
     1321 tgtccactat tagaagattg tagagaagga caaaaacgtt ataaagctag tttgaaagaa
     1381 gcgtgataac atgacaaata ttacaaaaga ggtatttgat aatttagaac aagagattga
     1441 tgtatttgcc aaaaataaga cattaggttc tagtgaagca aagccctact tggatgaata
     1501 ccatagtaaa attattgatt attttaagca ggttaatgac ataactggca atattgattt
     1561 tgataaatta aatcaatatc ctgttgtgcc aatgaatttt aaagaaagat atgattatat
     1621 gattgaacgt aaatatcatt ttatgggcta tcgacaaatg aagaccttta aaactgaatt
     1681 gattaaaatg aatgctagtt atcaaacaag gttaaagaat aagcaggtat agacaagatt
     1741 gatcatttcc taatgttgaa aatatataaa actaattcat gcacacatat ttgtatttat
     1801 gttaggtgtc gtgtcattac aacgtcttaa tataaaaact gtccatctac atcataatgg
     1861 atatcttgag atatgcttta agatacctat ttcgaatcgt aggtggattt cttatatcta
     1921 tgattgtgaa aatatgttgc aaacatcttg atatttcact gattaacgta cacagggaat
     1981 tgataaacat ttcaatttaa aacagcgtct aagatgacta tgagaaaaat aatcataatt
     2041 ttaaaaatgg gaacaaccac ttttgcaatg tcaataattt taaatagcta aagatgtgaa
     2101 tatgaaaaac tatttagatg tttcaaaatg tatgtgaatg actgatttta aggtttctac
     2161 taatatagca atagcgtatg aatacttata aaaaaagcag gaagttttac cttcccacca
     2221 taaaagatga agaacctaaa aaatcctcag ctacgccata aatatggtat aactgaggat
     2281 tttatgttgt gtggattagt tgaatatacc tgttaatcca ccgctgtttc tagattgttg
     2341 agatctagta ttgttatttg attgtgcagt accactgctg tttgctgatg tgtcactacc
     2401 accgtgtgta ctacgatttg tagtgttgtt atcttgacta ccagaaacat tgattgatgg
     2461 gatactacca cttactgagc taggacgttt aaagtcttcg ccatctctag atgaaatttt
     2521 tgacatcaca ttttcatata agaactgtgg atattcttgt tggctatgtc ccacaaatga
     2581 gttttcacca tattgtttaa ctttactgaa gcccatccac actgacatag tgtattgagg
     2641 tgtaaagccg ttaatccaca cgtctttcgc tgcattatca ggtaaattat attgtgaata
     2701 agtttcagca ccgtaagtac cagtacctgt cttagcaccc atatttactc cagatacacc
     2761 atggccatat gcagaaccat atggtttaaa tgtacctttt agcatctcag ctaacatgta
     2821 tgcagtgtaa tcactcatcg ctttatggct agtatgatcg tattcgattg tttcaccatc
     2881 acgagtaact actttttgaa ttgaatgcgc gttgttataa gtaccaccgt tagcgattgc
     2941 agcaaatgct gatgctaatt gtgttggtga gaattctgaa gcagaaccac caagtacttc
     3001 agatggacca atatcgcctt cgtagtttaa gccaagtttg gcagcgaatt tcttaggtgc
     3061 atcattacca gcattttgct taactgattg ccaagctttt aaagctggta tattgaaact
     3121 ttgtcgtaaa gcatcataaa tagatacagt accgtgactc ttcgtatcat agtttctaaa
     3181 tgtagaacca tcaacttgat atgaagattc atcttgaatc gcatggtttg ttgcccattt
     3241 catattttca atggcaggtc catacgctaa gaaaggtttt aaagatgaac cagtagggtg
     3301 aggatctgtt gcttggtttc tgttaacgac gtctttgaaa tcacgtccac cagatatagc
     3361 aactaaacca ccagttttac tatcaagaat cgttgcacca acttgttggt ctttattctt
     3421 gtagaagcta ccattatcaa catcattttg taatgttttt tgaacatctt tatccatgtt
     3481 tgtataaatt ttaataccac tttgtaatac attacctaaa ttttcatctt tgaatgcttt
     3541 attattcatt aattcagatt ttacaaagtt aacgtatgaa ttatactcag aatcttgatt
     3601 tgtatcaatg ttttgacgtt cttcagcagt acgatttact aagttcgctt ttaaatcgat
     3661 tttcttagca tcttcccact gtttatctgt aatgcgttta tgataatgca ttaagtataa
     3721 aacagtgttt ttacgatctt cagcagcttt tggatgatca taaatattat agttgtttgg
     3781 aacctgaggt aaaccggcta aataagcttc ttccgctaag tttaaatctt ttaaatcttt
     3841 attaaagtaa tacttagcag cagctttaat acctgttacg ccatcagaat agtaaatttt
     3901 atttagatat acttggaaga tatcatcttt actatactct tgttctaaac gatatgataa
     3961 gtatgcttct tgagctttac gtccaataga tttatgttgt gataaaaatg catctttaac
     4021 aacttgttgt gttaatgttg aggcaccttc agaaccaaat ccaccagtca agttcttacc
     4081 aattgcaccg aataaacgtt tataatcaag tgcgccatgt tcgtagaaac gattgtcttc
     4141 agttgcaagt actgcgtctt tcattgattt cggcacgtct tttaaattta catgctcatg
     4201 tctttggcca ttatctaatg ttttaacaag ttctccgttc ttgtcatata tctttgcagg
     4261 aatcggatct tgtaatttag cttcggtaaa agcaggtgct ttccaagcat aataagcaaa
     4321 caataagata cctagtaaaa gaacaacgaa aaatgcaata atcatgaagc caataatctt
     4381 aataatcgtt ctcttcacat ttctattctt ttttgagttg gatttcccac cattattacc
     4441 atttttctta ggctgagaag atcctttgtt ttccgtcata cgcggtcctc actttcatct
     4501 aatatcaact tatcaactgc ttttagatag tctaatcttg gttgatactg ataaggaata
     4561 tggtaaccat tttttcgtat ttcatcaact gttatagact ttttaatatt atctttatat
     4621 ctcttccaaa atacttcgaa ttttgaatag ggtaaaagat aaacttcatc tagcgtttta
     4681 aaacgaatca ttaaaaacac aatacctttt tgttgatatg catttttcat atgttcgact
     4741 tgatggtcat gaatattatt taaaggaaag gacgtcttgt ttttagtttc ctttgcttca
     4801 aaatcaatat aataaccttg ataaacgccg ttgtaatcag ttgttgaagg tgtacgaaaa
     4861 taagcttcgt taatcacagc tttactccgc ttaggatagt tgacattaac tatttgtact
     4921 ggcgtaggct ttttgtgaat aactgcaatg tcgcttttaa gataaaacgt atttgaatgt
     4981 tcgatatctt tttcaagtga cataccacgt ccaccatact caatattact aaaggcagcg
     5041 gtctttttcc ctccgtctat agcactattt ttacgatatg gtttaccatt tggataattc
     5101 ataaatttca ccacacaagt ttggtagtaa acgttgttta ttatacacaa cattatagtg
     5161 aaacaccaaa cttaaattag tataaatgcg aaagacacaa tacaccaaag cttattttaa
     5221 cgtgatttca gcattaacac aataatatta taatacattt accgttaatg ttggaatttt
     5281 acacacaatt ttaattaatt gaaaagtttt gcttataaaa atgaatactc attacaatat
     5341 ttttgaattt tgttataaaa gtcaaatcat gatcagtaat tattattttt gaggcaagta
     5401 caagtatg
//
LOCUS       DBNENW010000156         5357 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNENW010000156 DBNENW010000000
VERSION     DBNENW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.158"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..333
                     /locus_tag="AC1LW8_002313"
     CDS             <1..333
                     /locus_tag="AC1LW8_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4774107.1"
                     /translation="FIKINDSNYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLR
                     KITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYY
                     TYNHIMHH"
     gene            767..1369
                     /locus_tag="AC1LW8_002314"
     CDS             767..1369
                     /locus_tag="AC1LW8_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774108.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSALLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNIMER"
     gene            1636..4788
                     /locus_tag="AC1LW8_002315"
     CDS             1636..4788
                     /locus_tag="AC1LW8_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIP4774109.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4845..5327
                     /locus_tag="AC1LW8_002316"
     CDS             4845..5327
                     /locus_tag="AC1LW8_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774110.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         2056 a          656 c          970 g         1675 t
ORIGIN      
        1 ttcataaaaa ttaacgactc aaattataga cttagtaatg ttgatactat gaaagtaacg
       61 ctttacagta atggaagtaa ttatgataaa gaggctttac taataaataa agatgagttt
      121 tgtcctttaa ggaaaataac tttagataat aagcttgatt cacaacgggt aatggaaatt
      181 gattcattgg cagcaataat aaacttagtt aaacaaggaa aaggaaaggc tttattacct
      241 atgacttttg agaataaaag agatatagta caggacattt ctaagatatt tgaagttaac
      301 tactatactt ataatcatat tatgcatcat taattgaaag ttgattgagt ttaaattaca
      361 ggaacgattt taaaagtcta gtagttagaa tgagcgatgt gacagaaaag aaattatgcc
      421 ttttattttg taaattcgtt gttttaaagt aaaggtcctt gtagtatagg aaaaagtgga
      481 tgacttctcc gcctttacca ccctttcaag cataatgata actgctaaat attgattata
      541 gcaccattgc aaaactaatc attaattgtc atattcaact attttgaaaa gtatcaattt
      601 cacattatca aattattgag tatctcaatt tattgttata cacaaacagt atttttgtta
      661 tcatagagtt aataaatata ataaattaaa caagtaactt tacaaaaatt agaatgattc
      721 aattcaatca taattgtttg aaaatagagg tgagcaggtg aacgatatgt taattagtct
      781 tgtaatccca gtttttgctt tgttagttat tggtggtatt atttggatga ttatagaagg
      841 tatagtacat atttcaaaaa agaataaagc aattgataac ttttttaacc aagttaataa
      901 agtaagtgag acatataaat tcgctactac ttttttattt ctaatcttgg ctacggcggg
      961 tatttctcaa ttttatctat attatatagt atcagcgtta cttttttggc ttgtcatact
     1021 tacctttggt attgcaggta tcattttttt aatgccatat ggattatgtt ttctaccgtt
     1081 ttataagcaa aaaaagaaaa aacagacatt taaaaaatac atggtttaca ctacgattgg
     1141 tttgtcaatt tgtctaggct tatctctagt tttggttcac actacgaaaa tttatatgga
     1201 cgaaggtggc gtaagatact attacggtag ttttgtaatg aaacaagcgg gcggttatgc
     1261 ttatttagct ttagcagtac tttcaacgtt gttaattgtt gcgaaaaaag ctacaaataa
     1321 aaataaagaa atcgaaaccg tcgacaatac aaatataatg gaaagataat taagggagtg
     1381 ctcattcagg agtgctcttt tttgatgttc aaatttagtt ggaaatgaag gcatagaaga
     1441 aatttttata gtcatttatt ggataatgaa ttatggtcat atcgattaat tttattagtg
     1501 aggattttac aaacatttgt tgaataaata tggtaatgat aatatatgaa tatttttgaa
     1561 aaaacactaa tatgattaaa ttatttgtta aaatggcatt aagtaaaaaa caaacaggga
     1621 acaaggtggg atttcatgag tcaattgcta aatgatacgt tatcggcttg gttgttaatt
     1681 gaatctttaa gtccaggaga agtaaatttt acagcggaag atatactctc agctgaacat
     1741 tttaaaaatg gtgcaaagca agcacaactt caaagttttg atgaatattt tgaaatatgg
     1801 aatgctgaac gctttattat atcagaagaa aaatcagaga ctggggaact tatatttaaa
     1861 ttttatagac attgcttccg ctataatgaa attaatttga aaattcagga tatttttgat
     1921 gattattctg agattcataa tccaaatggg acacactgtt atggttacac atttaacaca
     1981 gataaacacg gcaaagtgat agttgattct atacatattc cgatgattat gagtgcatta
     2041 aaagaaattg aaaagaacaa aaatgccaat atagaagaaa aatttaatga ttctgttgaa
     2101 aaatttgttc aaaaagtaaa agaaatttta gcagatgaac caattaatga atttaaattg
     2161 aagaagatgg acaaagctta tgatgagtac ttttctgtat taaattcaaa gaaagatgga
     2221 ttatttggac attatgtagc aatagaatat gtgaaagata gtgatttacc acagccggaa
     2281 tttaacagct tcttcataag tgatattgag aaagcaagaa aatctcccaa ccaaacttta
     2341 attgattaca ttgaaggtgt agaagaaagt cagcgcatag aagtagatga aaataaagaa
     2401 atgtttgaca aatttttaca tccttcacgt ttgcctgatg gacgatggcc atcacagact
     2461 gagtttagat tgtctttaat gcaacaactt gctgtaaacc aaattacgag tggtaatgaa
     2521 agaataagtt cagttaatgg gccaccaggt acaggtaaga ctactttatt aaaagatata
     2581 tttgctcatc tagtagttga aagaggtaaa gagttagcta aactaaataa tcctaaagat
     2641 gcatttgtca aaacaaaaat tcatgaaacg gatgataaat acgtatactt actaaaggaa
     2701 tctattgcca aatataagat ggtagtcgca tctagtaata atggagctgt tgaaaatata
     2761 tctaaagatt taccgaaaat tgaagaaatt ataagaaatc ccgaaaaatg taaattccct
     2821 aaatatgaac agaattatgc aaatttagca catgaattaa aagattttgc tgaaatagct
     2881 gaagatttga ttggtgaaag tgcctggggc ttattttctg gagtttttgg taaaagtact
     2941 aatattaacc aagtattgag tcatatgtta aaacaagatg cgaatgatat tggctttgct
     3001 aaattactac aaaatgagaa taatcgtatg agttataacg agttaatgag tgaatggcaa
     3061 tcacatcaac gtgcattttt agaagagttg aggcatgttg aaatgttaaa agaagaatct
     3121 attagagcat atgatgttta taaaaattgt gagtctttct ctaagattga acaggttatt
     3181 aatagtgaaa aaacaagtat tgaagaacag gtatatcatt tagataatga aacgttacga
     3241 gacaataaag aaatagaaga tttggataat cgaattaatt atattgttaa gcaaatagaa
     3301 actttaaatg agttaattaa atccatcaaa gaaagcaaca aaggttttat taacaaactg
     3361 aaagcgatgt ttaattcaga agaagatgaa agctataaag atcataataa agagaagcaa
     3421 caattattaa cacaacagtt agagttagag aaatgtaaaa aaaacaaaca tgaagacctt
     3481 gttagcaaac taaaagaaaa agagaaatta attaaacaat taactaaagt acagttgcaa
     3541 ttagacgagt taaattcaca gttacaagag ttagaagcat atcgtattga gtcaaaaatt
     3601 acaattccag aaaaagattt ttggagtgac aacaattatg atgagcgcca agttactaat
     3661 ctgtggacga gtgacgaact tcaatacaga cgtgccatgc tctttttaag agcaatgata
     3721 ttgcataaat tattattgat tgctaataat acaactattt attatgcgat taatgatttt
     3781 aaagatagaa ggaaattaat tgatgcaaat ccagataaag tacacaacgc atggaatgtg
     3841 atgcatttaa tatttccagt agttagtacg acgtttgcaa gctttaaatc tatgtatggg
     3901 ggcataccaa aagatttcat agactactta tttattgatg aagcaggaca agcaatacct
     3961 caagcagctg tgggagcatt atatcgttca aaaaaagttg tagctgtagg tgatccgatt
     4021 caaatagaac cggttgtgac tttagaaagt catttaattg ataacattcg taaaaattat
     4081 catgttccgg aatatctagt ttctaaagaa gcttctgtgc agtctgttgc agacaacgcc
     4141 aatcaatatg gtttttggaa atctgatgct actgatagta atcaaaaaac ctggataggc
     4201 atacctttat gggtgcacag acgatgttta aaacctatgt tcacgatagc taaccaaatc
     4261 gcttataata ataaaatggt gttgccaagt aatattacaa aagtaggtaa aacaggttgg
     4321 tatgacgtta aaggaaacgc agttcaaaaa caatttgtga aagagcatgg tgaaaaagta
     4381 gtgggattat tagctgatga ttggattgaa gcaattaagg aaggtaaaaa tgaaccgagc
     4441 tcatttgtaa tatcgccttt ttcagcagta cagcaacaga ttaaacgtat gttaaagcaa
     4501 caactaccga ctagaattga tattgaacgt acaaaaatta atcaatgggt cgataaatcc
     4561 attggtactg ttcatacttt tcaaggtaaa gaggctcaga aggtgtattt tgtaataggt
     4621 actgataata cccaagatgg tgctgtgaac tggtcatgcg aaaaaccaaa cttgttaaac
     4681 gttgcagtga caagagctaa gaaagagttt tatgtaattg gcgacatgca aagaatacag
     4741 atgaaaccat tttatgagac gatttttaaa gaaagaaatg taaaataaca tacaaaaaag
     4801 tatatagagg aagttataca ttttaaaagg ggcaaaattg aataatggag aaatttaaca
     4861 actggatatt aaatgcaata agtggatctc aaacagacaa gaatgaaaca actgaagaat
     4921 taaaaggggc aaaatttatc attttatatg catattcaat gctcgttttg cttgcgttag
     4981 taatttctaa catattcatt cacattttgg agcctaaact atcaatcacc actcaaatca
     5041 tcatcgtttt gattttaatt gaagcactaa ttggactgcg tttcttgaaa gcgtacgatg
     5101 ttaagcgtgg caaagataaa gaaaataaga aaaatagtaa ggatttcgtt aaactaaaat
     5161 caattttagt agcaatttta tttacatcat tggcgctgac agcaggtact gtagctgata
     5221 tatacggttt cactgactta ggaaatacta gaagtgattt aatcgtttgg agcataggtg
     5281 gtattatatt tggcctcgta tgttacacaa tggaagataa aagataacga taaggagctg
     5341 gcgattataa agctagc
//
LOCUS       DBNENW010000157         5211 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNENW010000157 DBNENW010000000
VERSION     DBNENW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.160"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..489
                     /locus_tag="AC1LW8_002317"
     CDS             <1..489
                     /locus_tag="AC1LW8_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HIP4774111.1"
                     /translation="RFIDFEKESTQKYKKIYARFLVIINVLTTITVVFALCNLYYFAA
                     VQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHFGLLLTAL
                     SSFIYIEKIIEYILIPNVVESKFIIIVSMMVIAGAQFVAFQFIMQYSRFYIFELNTED
                     DD"
     gene            636..1247
                     /locus_tag="AC1LW8_002318"
     CDS             636..1247
                     /locus_tag="AC1LW8_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HIP4774112.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVVIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAFRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLIIRVRKGAFAFVVIRPILFSCWLFILNMILSRLV"
     gene            1446..1946
                     /locus_tag="AC1LW8_002319"
     CDS             1446..1946
                     /locus_tag="AC1LW8_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774113.1"
                     /translation="MTFEEKLNEMYNEIANKISSMIPVEWAKVYAMAYIDEESGEVFF
                     NYTEPRSDELFYYTSVIKKYNISRSGFMNSVYELHDQFEKLRELFIEEGHEPWTSCEF
                     DFTKEGKLKVSFDYIDWLNTEFDQLGRENYYMYKKFGVIPEMEYEMEEIKEIEQYIKE
                     QDEAEQ"
     gene            1957..2457
                     /locus_tag="AC1LW8_002320"
     CDS             1957..2457
                     /locus_tag="AC1LW8_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774114.1"
                     /translation="MTFEEKQSEMYNKIANEISGMIPVEWEKVYTIAYVDNQGGEVIF
                     NYTKPGSEELNYYSDIPKDCNVSNDIFMDLWMKVYRMFDELRETFKKEGHEPWTSCEF
                     DFTRDGKLKVSFDYIDWLNTEFDQLSLENYYMYKKFEVIPEMEYEMEEIKEIEQYIKE
                     QEEAEI"
     gene            2468..2968
                     /locus_tag="AC1LW8_002321"
     CDS             2468..2968
                     /locus_tag="AC1LW8_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774115.1"
                     /translation="MTFEEKLNEMYNEISNKISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPNSDELNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRNLFKEEGLEPWTSCEF
                     DFTREGTLNVSFDYIDWVNTEFDQLGRENYYMYKKFGVIPEMEYEMKEIKEIEQYIKE
                     QDEAEI"
     gene            2979..3479
                     /locus_tag="AC1LW8_002322"
     CDS             2979..3479
                     /locus_tag="AC1LW8_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774116.1"
                     /translation="MAFEEKLNEMYNEIANKISSMIPVEWETVYAMAYVNERSGEVFY
                     NYTEPSSDELFYYTSVLNKYNIPRSEFMDSVYELYKQFDNLRELFIEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVKKIKDYVKE
                     QDEAEL"
     gene            3490..3990
                     /locus_tag="AC1LW8_002323"
     CDS             3490..3990
                     /locus_tag="AC1LW8_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774117.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYTIAYVDDQGGEVVF
                     NYTKPGSEDLNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPSGKENYYMYKKFGVLPETEYEINKVKEIEQYIKE
                     QDEAEL"
     gene            4001..4501
                     /locus_tag="AC1LW8_002324"
     CDS             4001..4501
                     /locus_tag="AC1LW8_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774118.1"
                     /translation="MTFEEKLSQMYNEIADEISGMIPVEWENIYTIAYVTDQGGEVIF
                     NYTKPGSDELNYYTDISRDYNISEEIFDDLWMNLYYLFMNLRDLFKKEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEVKEIEQYIKE
                     QDEAEL"
     gene            4512..5000
                     /locus_tag="AC1LW8_002325"
     CDS             4512..5000
                     /locus_tag="AC1LW8_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HIP4774119.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTREGNLKVSFDYIDWIKLGFGLSGKENYYMYKKFGVLPETEYEMEEIREVEKYVKD
                     QE"
BASE COUNT         1998 a          497 c         1028 g         1688 t
ORIGIN      
        1 agatttatag acttcgaaaa agaaagtaca cagaaatata aaaaaatata tgctcgattt
       61 ttagttatta taaatgtgtt aactactatc actgtagtat ttgcactgtg taacctttat
      121 tattttgcag ctgtacaaaa ccattacgat ttattcaatt attggttaat gggtacgatt
      181 tcaatcataa ttagctattt attattagta attggcggaa tgtttacgtt gttaaaatta
      241 cctaaagtaa caaaacgctg ggggggtaaa actaaaacac atttcggttt attattaact
      301 gcgttgagct catttatata tattgaaaaa attatcgaat atatattgat tcctaatgtc
      361 gtagaatcca agtttataat tattgtgagc atgatggtta tcgccggagc acaatttgtg
      421 gcatttcaat ttattatgca atacagtaga ttctatattt ttgaattaaa cactgaagat
      481 gatgactaaa tgaatttata aattactgtt aaagtatact taaataagaa aatcaacttc
      541 aacgtatatc gccatattat tttatactca atgatgtgaa tggatttaag tggattgata
      601 ttaaagtaaa agttatggaa tattgaggtg cgaatatgga gttcttatta ttaattgtcg
      661 tagccggact gtattatatt atatatttaa ctgctgtgat gtattctgaa aaaatagtag
      721 tattgcctat aatcatctat gccattgtgt ttgtagtaat tggtatcact tatattttta
      781 taggtgacag ctatgatcag ttaacaaatt tcaatgtgat tttgtatatg gggagtttgt
      841 tttatgcatg gatggctttt agaaatcttt ggaacagacc attattatta aaatataaga
      901 acattacaga tagttcaagt ggaatagtta ataaatctga atataattca gttgaaagct
      961 tacgtataaa cattgaaata gctaagtata aagggattat ttctttgata gtagctatag
     1021 tactaacggt attaatgaca ttaaagtcaa cgcctcaaat tactgcggaa acacgtgact
     1081 taagtatctc atttttcata ctcagcttat ttatcattat tatatttgct gtttgggatt
     1141 taattattag agttagaaaa ggagcgtttg cttttgttgt aataaggcca atattattca
     1201 gttgttggtt atttattctg aatatgattt tatcaagatt agtataatga taagaagttg
     1261 aataaattgt aatgtgaaaa atcattgaga atttctatta ttgtaacaat agtttcagtt
     1321 attttagttt cattagttta tttaggttta aaatgagtaa tattattttg aagaagcatt
     1381 aagccacgta gaggtgttgt ttaatgcttt ttatttaacg gtaatgaaaa tataggggag
     1441 ataacatgac tttcgaagaa aaactaaatg aaatgtacaa cgagattgcg aataagatta
     1501 gcagcatgat accggtagag tgggcaaagg tatatgcaat ggcatatatc gatgaagaat
     1561 ctggagaagt gtttttcaat tatactgaac caagaagtga tgaattattt tactatacaa
     1621 gcgtgataaa aaagtataat atttcgagat caggatttat gaattcagta tatgagttgc
     1681 atgatcaatt tgaaaaacta agagaattgt ttatagaaga aggacatgaa ccatggacat
     1741 catgtgagtt tgattttaca aaagaaggta aattgaaagt atcttttgat tatatagatt
     1801 ggttaaatac agaatttgat caattgggtc gtgaaaatta ttatatgtac aaaaaatttg
     1861 gggttatacc agaaatggaa tatgaaatgg aagaaattaa agaaatcgag caatatatta
     1921 aagagcaaga tgaagctgaa caatagaggc gataacatga ctttcgaaga aaaacaaagt
     1981 gaaatgtaca ataaaattgc aaatgagatt agtggaatga taccggttga gtgggaaaag
     2041 gtatatacaa ttgcttatgt agataatcaa ggtggagagg tcatttttaa ttatactaaa
     2101 cctggaagtg aagaattgaa ttattattca gatattccta aagattgcaa tgtctcaaat
     2161 gatattttta tggatttatg gatgaaagtt tatcgaatgt ttgatgagtt aagagaaact
     2221 tttaaaaaag aaggacatga accatggaca tcatgtgaat ttgattttac aagagatggt
     2281 aaattgaaag tatcttttga ttatatagat tggttaaata cagaatttga tcaattgagt
     2341 cttgaaaatt attatatgta caaaaaattc gaggttatac cagaaatgga atatgaaatg
     2401 gaagaaatta aagaaatcga acaatatatt aaagagcaag aagaagctga aatatagggg
     2461 agatgacatg actttcgaag aaaaactaaa tgaaatgtac aacgagattt cgaataaaat
     2521 tagtagcatg ataccagtag aatgggaaaa ggtatataca atggcttata tagatgatgg
     2581 aggaggtgaa gtattcttta attatactaa accaaacagc gatgaattga attattacac
     2641 cgatatacct aaggagtata acatttctgt gcaagtattt gatgatttat ggatggattt
     2701 atatgatttg tttgaggaat taagaaattt atttaaagaa gaaggactag aaccatggac
     2761 atcatgcgaa tttgatttta caagagaagg tacattgaat gtatcttttg attatattga
     2821 ttgggttaat acggagtttg atcaattagg ccgtgaaaat tattatatgt acaaaaaatt
     2881 tggggttata ccagaaatgg aatatgaaat gaaagaaatt aaagaaatcg aacaatatat
     2941 taaagagcaa gatgaagctg aaatataggg gagataacat ggctttcgaa gaaaaactaa
     3001 atgaaatgta caatgagatt gcgaataaaa ttagcagtat gataccggta gaatgggaaa
     3061 cggtatatgc aatggcttat gtaaatgaaa gaagtggaga agtgttttac aattatactg
     3121 aaccaagcag tgatgaatta ttttactata cgagcgtgtt aaataaatat aatataccaa
     3181 gatcagaatt tatggactca gtatatgaat tatataagca atttgataat ttaagagaat
     3241 tgtttataga agaaggactc gaaccatgga catcatgtga atttgacttt acaagagatg
     3301 gcaaattgaa tgtatctttt gattatattg attgggtgaa ttcagaattt ggaccaatgg
     3361 gaagagaaca ttattatatg tataaaaaat ttggaatttg gcctgaaaaa gaatatgcca
     3421 taaattgggt taaaaaaata aaagattatg ttaaagagca agatgaagct gaactatagg
     3481 ggagataata tgactttcga agaaaaacta agtcaaatgt acaacgagat tgcgaataag
     3541 attagcagta tgataccggt agagtgggaa aaagtatata caattgctta tgtagatgat
     3601 caaggtggag aggtcgtttt taattatact aaaccaggta gcgaggactt gaattattac
     3661 acatatatcc ctagagagta taatgtctct gaaaaagtat tttatgattt gtggacggat
     3721 ttatatagat tgtttaagaa gttaagaaac gcatttaaag aagaagattt agaaccatgg
     3781 acatcatgtg aatttgactt tacaagagat ggtaaattga atgttgtatt tgattatgtt
     3841 gattggatga attcagaatt tggtccatca ggaaaggaaa actactatat gtataaaaag
     3901 tttggtgttt taccagaaac ggaatatgaa attaataaag ttaaagaaat cgagcaatat
     3961 attaaagagc aagatgaagc tgaactatag gggcgataac atgactttcg aagaaaagct
     4021 aagtcaaatg tacaatgaaa ttgcagatga aatcagtgga atgataccag tagaatggga
     4081 aaatatatat acaattgcct atgtaactga tcaaggtgga gaggtcattt ttaattatac
     4141 taaaccaggt agcgatgaat tgaattatta cacggatata tctagagatt ataatatttc
     4201 agaagaaata tttgatgatt tatggatgaa tctttattac ttgtttatga atttgaggga
     4261 tttatttaaa aaagaaggtc ttgaaccatg gacatcatgt gaatttgatt ttacaagcga
     4321 aggtaaatta aaagtttcat ttgattatat tgattggata aatacagagt ttgatcaatt
     4381 aggccgtgaa aactattata tgtataaaaa gtttggtgtt ttaccagaaa tggaatacga
     4441 aatggaagaa gttaaagaaa tcgagcaata cattaaagag caagatgaag ctgaactata
     4501 ggggcgataa catgactttc gaagaaaaat taagtcaaat gtacaacgag attgcgaatg
     4561 agattagtgg gatgatacca gtagagtggg aaaaagtata tacaattgct tatgtagatg
     4621 atgaaggtgg agaagtcgtt tttaattata ctaaaccagg tagtgatgaa ttgaattatt
     4681 acacatatat ccctagagag tataatgtct ctgaaaaagt attttatgat ttgtggacgg
     4741 atttatatag attgtttaag aagttaagaa acgcatttaa agaagaagat ttagaaccat
     4801 ggacatcatg tgaatttgat tttacaagag agggtaattt gaaagtatcg tttgattata
     4861 tagattggat taaattaggt tttggtctat caggaaagga aaactactat atgtataaaa
     4921 agtttggtgt tttaccagaa acggaatatg aaatggaaga aattcgagaa gtagagaagt
     4981 atgttaaaga ccaagagtag catatatgtt atagaaggct gtgcaaaatc accctagttt
     5041 tacatttgac tcaaagaaga aggtaaacga taagattatt tgcaacttaa aaagtctatt
     5101 agcttctcgg tatccataca tcatatgtat aaatgagttt aactaattaa taaatcacga
     5161 tataaattta aaattgccat taattgaaaa ggttattgta gtgtatttta t
//
LOCUS       DBNENW010000158         5208 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.161, whole genome shotgun sequence.
ACCESSION   DBNENW010000158 DBNENW010000000
VERSION     DBNENW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.161"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(75..259)
                     /locus_tag="AC1LW8_002326"
                     /pseudo
     CDS             complement(75..259)
                     /locus_tag="AC1LW8_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(348..1412)
                     /locus_tag="AC1LW8_002327"
     CDS             complement(348..1412)
                     /locus_tag="AC1LW8_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIP4774120.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="AC1LW8_002328"
     CDS             complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="AC1LW8_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HIP4774121.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="AC1LW8_002329"
     CDS             complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="AC1LW8_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HIP4774122.1"
                     /translation="MIVNYLKHKFYNLLTTMVVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3897..>5208)
                     /gene="sdrM"
                     /locus_tag="AC1LW8_002330"
     CDS             complement(3897..>5208)
                     /gene="sdrM"
                     /locus_tag="AC1LW8_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter SdrM"
                     /protein_id="HIP4774123.1"
                     /translation="LILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALV
                     IANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMS
                     LSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAI
                     IAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILA
                     IVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLG
                     LSPLQSGLVIFPLSLAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLP
                     VLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGY
                     LYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT         1864 a          975 c          665 g         1704 t
ORIGIN      
        1 atgttcaatc tcttttattc tacttcattt atttcttgaa gtcaataact ttttgaacga
       61 ttactttatt tattctatat tgtttttata gttatttcaa tggtaagttt tacacttttg
      121 aaattcttct ttaaaaacaa ctgcgtcgtt tttgacgact ttatcatatt atcaactttg
      181 gaatttaaag tcaataactt ttttaaaact ttttgtgttc acaacccgct tctttttcaa
      241 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      301 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      361 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      421 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      481 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      541 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      601 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      661 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      721 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      781 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      841 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      901 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
      961 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1021 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1081 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1141 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1201 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1261 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1321 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1381 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1441 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1501 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1561 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1621 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1681 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1741 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1801 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1861 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1921 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     1981 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2041 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2101 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2161 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2221 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2281 ataaccatac ttattatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2341 gcactactaa atgcgtatag cattaacccg aatccaatag ttgaatacat tactgagcgc
     2401 ttatctaatt tcgtatattt cacttcattg taacttgaga ttgaaactaa gccgaaaaca
     2461 aaggcaacta aagcaattcc tacaattata ataaatggta ctcgccaact cgcttgatca
     2521 ataattaatc ctgtaactgt agggcctatt gctggtgcaa attgaattac taaacctgct
     2581 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2641 aagggcataa gtacgcctgc acccatcgct tgaattacac gtgctaacat taaaactcca
     2701 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2761 tataaaggtc tagttttaat tctatccatt agatatgccg tcaaaggtat catgacgcca
     2821 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattaat atttaattct
     2881 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     2941 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3001 atcataattc ctccttttta aaacagatat acacttgtcc ctttaataca tatagccatt
     3061 aacataatgg gagtagtact caaatgataa agaagcacta cataataaat cattattggt
     3121 tcttacacat taggcacagc taatgtgtac ttaaaaaact aacaatataa cattgtaggg
     3181 cctaataatt tattttattt ctcagactca tacaatggtt atttaactta tttaccacaa
     3241 attgagcact aagactcact attatcgatt cttctattga acaaaaagaa gttggaacac
     3301 atatgtgccc tcaacttcag atatctattt tctattattt aaatttttac tttcagggta
     3361 aaattgaagc ttacaaacat ataagaaccc tataataacc atgatgaccc aaaaatcgat
     3421 attgatatac acatagttca gatgaataat aaaataaaat aacgttgttg caactgtgta
     3481 agaaatcaaa tgaaaagtcg cgcctctaaa atatgcattt tcaggcaaca tttttctaaa
     3541 ataatccatt aaatgctcga ctgcaaatat gatgaagtac ccaaagatta tataactgcc
     3601 ataatataat aggttatcgt acatacttct attatatgta aagtcaccca acctaaaata
     3661 aataagtatt ctacttaatc catataaacc aaaccctaga aatgttaaaa aaatcgcacc
     3721 agaaagtaca aaaataaaga gaacaaccat tgtagttaac aagttataaa acttatgttt
     3781 aagataattc acaatcattg gcctccttat taataaaata ttgaacgatt tatctagtat
     3841 ctaggaaatt tattatttct tactttaatt acactacatc aattttaatc ataattctat
     3901 tcttttgatt gagatgactg ttctttaaaa aatacgaccc ataaaacaat aagcccaatg
     3961 ctaattacag caacagcact taacacatta tgtaagtttg gaccaaagat tcctgattgt
     4021 atcgcataaa gatatcccat tattgttgaa cctattgatg ccccaagatt tttagttaat
     4081 ccataaaatg acatcatttt cttcatttgt aatgggctag tttcctcttg gacaattaca
     4141 ctatctttcg tataaatata tccaaaactt aatcctgcca aaatcaacac aaatgctata
     4201 agtaccggca atttaatacc aaatgaaatg ataatactac ttactagtag caatgtaaat
     4261 gatagtaagt aaataacttt ccttgatagt tttgcttcaa ttcgatgtaa attaaaattc
     4321 aatgtaatcc aagctaaaga taaaggaaaa ataaccaatc cactttgcaa tggagataat
     4381 cctagttgtt cttgaaggta gactggaata tataaattga atcccattaa acaaatagct
     4441 gttagaaggt cagttataaa aactaaagta atcgaacgat taaattcaac cacaggtaaa
     4501 aatggtgaag aaacatgttt ttctacttta aataaacaca ttgccacaac gatagctaag
     4561 ataaaactca agaaatttaa aagtaacagc tgttggttta ataaagcaaa cataatcaat
     4621 ccaataaata cataaaatag cgtgagccct ttagtatcaa attttgattt agcaactgtt
     4681 tcttcctctg gaaaatgaaa tgtccatata actaatataa tggcaatgat cgcaatcggt
     4741 atatttatgt agaataacca atgccatgtt gcaaattcta atataccacc acctagaagt
     4801 ggcccaatga tactcgaaat accccaaaca ctgccaacta tgcccataat tttatatcgt
     4861 aacggaattt caaatgctaa ctttggaaca atttgcgata atgacatcaa tacacctgat
     4921 ccaaaacctt gtatcacgcg tgatataatt agcatagtaa aattagtact taaaccacac
     4981 ataaaactac ctatactaaa taaaagtaat cctgcaattg ccacataaat aattttaaat
     5041 ctcgaaagta attcgcccac tataggattg gcaattacta atgcgataaa atacgctgtg
     5101 aaaattaatg aaattaaatt tcctgcattt aaatcttgtt taattgtagg taatgcaagc
     5161 gaaataatag atgattcaat cgctgacata aacataatta atattaaa
//
LOCUS       DBNENW010000159         5191 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.162, whole genome shotgun sequence.
ACCESSION   DBNENW010000159 DBNENW010000000
VERSION     DBNENW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.162"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            246..1343
                     /locus_tag="AC1LW8_002331"
     CDS             246..1343
                     /locus_tag="AC1LW8_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HIP4774124.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQHALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKLDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1548..3110
                     /locus_tag="AC1LW8_002332"
     CDS             1548..3110
                     /locus_tag="AC1LW8_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIP4774125.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFVIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3121..3228)
                     /locus_tag="AC1LW8_002333"
     CDS             complement(3121..3228)
                     /locus_tag="AC1LW8_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774126.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(3300..4094)
                     /locus_tag="AC1LW8_002334"
     CDS             complement(3300..4094)
                     /locus_tag="AC1LW8_002334"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIP4774127.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSEKETTFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(4114..5190)
                     /locus_tag="AC1LW8_002335"
     CDS             complement(4114..5190)
                     /locus_tag="AC1LW8_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HIP4774128.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
BASE COUNT         1746 a          773 c          939 g         1733 t
ORIGIN      
        1 agagcaaatc acgcaatcaa caaataatat aagaagtttt tgcgatagtt ttaaaatagc
       61 tgtaatagaa tactaaatgt gacaaactta gaactaatat caagtgttga tgttttgaat
      121 ataaaaatgc taatagatgg catcacgaga tttatgacta tctgttaaat caacctaaca
      181 cgattataat ttgaataatt ggttaatata tgagtaatta gaaaatagac aaaggatgac
      241 gatttatgta tatcaatatg aaagattatg ggttaacagg cataaacaaa actaaagata
      301 ctcgagcaat acaacatgcg ttaaatcgtg gaagatgtaa accaacgaca gtttatatac
      361 cgaaagggac gtatgatatt tgcaaaccat taacgatata tggcaataca acacttttgt
      421 tggataatga aactatttta cgccgatgtc attctggtcc tttattaaaa aatggtcatc
      481 gctttggttt ttatcgtggt tataatggac acagtcatat tcatattaaa ggcggcaagc
      541 ttgatatgaa tggcgtatcg tatccttata acaatacagc tatgtgcatt gggcatgctg
      601 aagatattca attaataggt gtgactatta agaatgtagt gagtggtcat gcaattgatg
      661 cttgcgggat taatggactc tatattaaaa gttgttcatt tgaaggattt atagactata
      721 gtggcgaacg cttttattct gaagcaatac aattagacat tcaagtacct ggtgcttttc
      781 caaaattcgg aacgacagat ggtacgataa cgaaaaatgt cgttatcgaa gattgttatt
      841 ttggaccttc agaattgccc gaaatgggaa gttggaatcg tgctattggc tcacatgcaa
      901 gtagacataa tcgttactat gagaatattc atattagaaa taatatattt gaagatatac
      961 aaggttatgc attaactccc ttgaagtata aagatgcttt cattattaat aataagttta
     1021 ttaactgtga gggtggtatt agatatttag gagttagaga tggtaaaaat gcagcagatg
     1081 tgatgacagg aaaagactta ggttcccaag caggcataaa tatgaatata attggaaatg
     1141 aatttaaagg atcaatgtct aaagatgcga tacatgtacg taattataat aatgttaaac
     1201 ataaagatgt attaatcgtt gggaatacat tcaataattc gactcaatca attcatttag
     1261 aagatattga tacagtgttt ttaagtcctg ttgaagcggg tattcaagtt actacaatca
     1321 atgtagatga aataaaaaag taaaaagttt cgcatgacat taggattaag aatagtagat
     1381 aatttttgaa agcgcattca taaaacggta taaatatgct ataataaacc caattatctg
     1441 atgaaagggg tattttgacg gtaatgataa tacaagatag acaactttct atactctaat
     1501 atagtgagtt gaagtagctt gtcataatca tcatgagggg gaaatttatg gcttatttca
     1561 atcaacatca atcaatgata tcgaaaaggt atttaacatt cttttcaaaa tcaaagaaaa
     1621 agaaaccgtt tagtgcggga caacttattg gactaatatt aggtccatta cttttcctat
     1681 taacattatt attctttcat ccacaagact taccttggaa aggcgtatat gtcttagcga
     1741 ttactttatg gattgcgact tggtggatta ctgaagcaat tcctattgca gcaacgagct
     1801 tattaccaat tgtgttatta ccattaggtc atatacttac accagaacaa gtatcatccg
     1861 aatatggcaa tgatattatc tttttgtttt taggtggatt tattttggca attgcaatgg
     1921 aaagatggaa tttacatacg agagtagcgt tgacaattat taatttgatt ggtgcaagca
     1981 cttcaaaaat attacttgga ttcatggtgg caacaggatt cttatctatg tttgtatcga
     2041 acactgcagc tgtaatgatt atgattccga ttggtttagc aattattaag gaagcacatg
     2101 atttacaaga agccaatacg aatcaaacaa gtattcaaaa gtttgaaaaa tctctagttt
     2161 tagcaattgg ctatgcaggt acgattggtg gcttgggtac attaatcgga accccgccat
     2221 taattatttt aaaaggacaa tatatgcaac attttggaca tgaaattagt tttgctaaat
     2281 ggatgattgt agggattcca acggtcattg ttttgttagg tattacttgg ctctatttaa
     2341 gatatgttgc gtttagacat gatttgaaat atttacctgg tggtcagacg ttaattaaac
     2401 aaaagttaga cgagcttggc aaaatgaagt atgaagaaaa ggtagtacaa actatctttg
     2461 tacttgccag cttattatgg attacaagag agtttcttct gaaaaaatgg gaagttacgt
     2521 catccgttgc agatggtacg attgctattt ttatatcaat attattattt gttattccag
     2581 ctaaaaatac tgaaaaacat cgccgtatca ttgactggga agttgcaaaa gagctccctt
     2641 ggggtgtatt aattttattt ggtggcggtt tagcattagc gaaaggtatt tctgaaagtg
     2701 gtttagcaaa atggttaggc gaacagttga aatcattaaa tggtgttagt ccgattctta
     2761 ttgtaattgt cataacaatc tttgtcttat ttttaactga agtgacatct aatactgcaa
     2821 ctgcaacgat gattttaccg attttagcaa cgttgtctgt tgctgttgga gtgcatccat
     2881 tactacttat ggcacctgca gctatggcgg ctaactgtgc atacatgtta ccagtaggga
     2941 caccaccgaa tgcaattatc tttggttcag gtaaaatatc tatcaaacaa atggcatcag
     3001 taggattctg ggtaaactta atcagtgcaa taattattat tttagtcgtg tattatgtaa
     3061 tgcctatagt tttaggtatt gatataaatc aaccactgcc attgaaatag taattgcaga
     3121 ttagaacgaa aaataaaagg ttacattagc aattgcttga acgagtggta atgaaacgta
     3181 tacagcagca tcgtgtaaaa acaatacaaa caaaagaaag tcaaccaagg atggattcct
     3241 attttaatcc ttggttgact ctttatttta tttaaattgt agaacctaga aaataaagtt
     3301 taattaaaag caccaatcat ttctactttg aaatctaagg tttctaaaat agcaatgact
     3361 ttctttatat cggttgtaat tgcagaatca gcctgaacga aaaatcgata catacctaat
     3421 tgtgttttta aaggacgaga ctcaatccag gataaattaa tattaaacaa agcaaatgta
     3481 ttaagcacac ttgctaacaa cccaggttta tcatgcattg gtgtaattaa aaacatcaat
     3541 gatgtcgcat tttgatcaaa ttgctgctga ttttttataa ctaaaaaacg tgtcacgtta
     3601 tgtggatagt cttcaatatg tgtatcaata ggtgtaaaac cataagcttc gccactacct
     3661 aaaggtgcaa ttgctgcaac gccattttca attttagtca aactttgaat tgtactgtcg
     3721 acataatcat agtcaaattg atgttgatgt atgtaatttg ttgtttgact gattgctggt
     3781 gcaatagaat acactttttt aatatcagaa atggaatctg ttccattacc atataatgca
     3841 aagttaatat ctaaacgtat ttcaccgtgt gcaaagacat cttgctgtgc aagtgcatct
     3901 gccacaatgt tgattgttcc ttctatagaa ttttcaatag ggacaacacc aatcgatgtg
     3961 tcatcatctg caactgcctt gatgacttca aataaatttg actttggttg aaaagttgtt
     4021 tccttttcag aaaaatactg acgacaagcc aaatatgaaa atgtaccttt agggcctaaa
     4081 taatataatt gcatatgcta cacctctact aacttaatga tggaaagggc actggttagc
     4141 atttgattct ttctttttat agaaaaagtt tggatctttt actgtattgt catatccatg
     4201 atgataattt gacgtcaatg ttggagataa tggcggcgct agccaagacc attttccggt
     4261 aacttgacga ccttgttgtg cttcattacg ttcgaatagt tcgaattgct ttgcagcggt
     4321 caaatgatcg acaattgata cgccttcttt tttaaaggaa tgatacacag catagttcaa
     4381 ttcaacaagt gctcgatctt tattaaatga attattttta agtgtatcaa attcaaacgc
     4441 atctgcaact ttttctagta aattgtaacg gtaatcatca ataaagttac gtacgccaat
     4501 ttcagttacc atataccaac cgttaaaggg tgcagttgga tatacaatgc caccgatttt
     4561 taagtccata ttggaaatga tagggactgc ataccatttt aagttcaatt ttcttaattt
     4621 tggataatga ttatgttcaa taggtacttc tttaattaat gaagtaggat attcgtaaaa
     4681 tttaactgac tcattaggta attggtaaat cagtggtaac acgtcaaaat tagtaccttt
     4741 tcctttccaa cctaagtgat ttgctaagcg tgtaacttct ttttcagcag gatcaccaca
     4801 attgtcatag ccagcatagc gaattaattg attgttgaaa attttaggtc catcctttgg
     4861 agcatatata gtaatatacg gctttaattt accttcattt gtagcctgtg taatatgata
     4921 agtaattgat gataagaacg atgcttcgtc agtaacatct cttgcatcaa tgacatttaa
     4981 cgaatcccaa aataaacgac caatgcaacg atttgaatta cgccaagcca ttttagcacc
     5041 ataaataagt tcttcttctg tatgtgtata tgtcccagtt tcttttattt ctagttcaat
     5101 gtcatgtaaa cgtttattga taatttgcgt ttcataatga cactctttat acatgttttc
     5161 tatgaaagct tgagcctctt taaataacat t
//
LOCUS       DBNENW010000160         4930 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.163, whole genome shotgun sequence.
ACCESSION   DBNENW010000160 DBNENW010000000
VERSION     DBNENW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.163"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            81..2186
                     /gene="rsp"
                     /locus_tag="AC1LW8_002336"
     CDS             81..2186
                     /gene="rsp"
                     /locus_tag="AC1LW8_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HIP4774129.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTRSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            2257..2679
                     /locus_tag="AC1LW8_002337"
     CDS             2257..2679
                     /locus_tag="AC1LW8_002337"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIP4774130.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
     gene            2944..3300
                     /locus_tag="AC1LW8_002338"
     CDS             2944..3300
                     /locus_tag="AC1LW8_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HIP4774131.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(3571..4848)
                     /locus_tag="AC1LW8_002339"
     CDS             complement(3571..4848)
                     /locus_tag="AC1LW8_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIP4774132.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT         1798 a          777 c          713 g         1642 t
ORIGIN      
        1 tactatccat gattttaaaa atttccatgt tttacattta aatgatagat ttcaatatat
       61 attaggcagg agagagaaat atgacatgcc aacttaaaat acatcataca ttatcgacaa
      121 tacccagtcg taatattaat aatattatgg tgctattttc acttacaagt aaactcaata
      181 ttacgattaa tggtgaaacc aaagacgtaa gtaattatat tatcttaatt aaccatggtg
      241 atatttataa tattaaccac ggcgaaaata taatcgaatt aatgataccc gttttttatt
      301 tttatcaaca agatgatgat ttctttaatg gttatttaga tcgtcattta ttacaatcaa
      361 gtaattatat taagtcatta attgccgatt taatcagtac accaacaaga tcttctttaa
      421 tggggaaaaa tataggtcaa agtattatag acactttact taaagaagct tttataagaa
      481 tagatcatga atatttacca aatattgctt taagcaatcc ggtgttcata gactgtgtaa
      541 actatattca tgacaatatt gatgcgcact tgtctttaaa agatatagcg atgcattgca
      601 acatctctga atcatattgt tctaatcttt ttgttcgata tttgagtatg aattttaaag
      661 attattttac aagtattaaa cttgttaacg ccatcaattt attactttct acaaaacatt
      721 ctattacaac tgtttctgaa ttggccggtt ttaacagtca tacaaatttt gcaaatcaat
      781 ttaagaatta cttgcatttt agtcctaaac agttccgttc actcgtttcc aagattactg
      841 aaccaccaca aatacacttc caacaagata acgtatcaca attcactgag ttaatttcaa
      901 caattgattt aacagctcaa ctggctacaa atacaacaga tattcatata gatgatttta
      961 atccgaaaga tcgaagtcag cgtgccaaag tatttgttcg atttagtaat ttcaatgaac
     1021 tatttcaatt tattttcaat gaatattacg atattaactt tgagcactta ccaaaacctg
     1081 tcgttttcat tgacgatatt cacgatattg agattagcca aacgaattac aacctgttaa
     1141 atagatgttt tgaaaagctt ttcgaaaaaa atataggttt agcaatcgct attaaatcaa
     1201 cacaacaatt cgaaacgatg aagcaattaa tactcacatt tttacaaggc aatcaagact
     1261 ataagacaag caagaaatta gttaaattta tgctcgtatt ttgctctaat tctatgacag
     1321 cagaagaaat tcatttatgt catcttaaaa ttaaaaacaa aaataaagaa attaaatata
     1381 gtgtgacagt cgatggcttt ttagaaactt attcaaccgt ggaacaagtt tatgatgtca
     1441 tgcagcgtct taaattccat tattacttta ttgatattga aaattcaaaa actgcaacac
     1501 atcttatcac taaaaaccaa cactatcatc aaactgacac tcattttgaa cagtataaga
     1561 aatttatttt agattcaggt atatcatcaa cacaatttgt atataataac ctgtctgtaa
     1621 gcggatttaa atatactaat gatggtaaga atccaattca attatctgac atagtgtatc
     1681 acttaattgc attattacga tatggcggtg gcattagcta tcaattatta gatgaccatt
     1741 caaattatat ttccttgtac aacaaatatg gtagtcccct accattaatg catttatata
     1801 aaatgtttag accttttgtt aatgaagata ttgaaattac aaataattat gtattgagtc
     1861 gtaaagataa taactaccat ttcttattat tcaataaaat taatgatcga tatatgtcag
     1921 acgtaaaaca agatttcatt ttccataatg aattacctca agactctttg atgattatta
     1981 aaacattgaa tcatgaacat ggttcaattc aacatttgct tccaataagc gatcaacttg
     2041 tttatataga aaaagaaatt ttagatgaat tagacaaaac gaattaccct aaaacggagc
     2101 ttgcagttca agaagaaact ggtcgtacat ttgaactcaa gttaaatcac gacgaggtta
     2161 aatatatttg ctttaaacca agctaaatac taacagtcct cttgtgttta gtttcttacg
     2221 ttaaaggcta tttatatcat aaggagatga tatgtaatga gtaactcaca agcaattcaa
     2281 gcaattgaaa acgtgttagc aacgtcaaaa gttggtgtat tatcaactgc atataataat
     2341 aaacctaata gtagatatat ggtcttttat aatgatggtc ttactttata tactaaaacg
     2401 aatatccatt ctgctaaggt caaagaaatt aaagataatc cagcagcata tgttttgtta
     2461 ggctacaatg atacgacaaa tcgcagtttc gttgaaatgg aagccacgat agaagtagtt
     2521 actgatcaaa aagtaatcga ttggttatgg gagactcaag ataaaagctt tttcagttca
     2581 aaagaagatc cagaactttg tgttttaaag gtgacaccac aatctgttaa attgatgaat
     2641 gacaaatcat tagatacacc tatcaaaatc gatttataac acaaagtata tataggaaat
     2701 aacttttatg aattctagat ataacaatgt taaatactta aaataactcg ctataattaa
     2761 agtgtttaat atgtttacaa ttcaatttgc aatgtattgt tttttagaca cactttattc
     2821 acatttaacg tgattagggt gtgtcttttt aaattaattt tatgtaatat aaatactgca
     2881 tttgcaaact gttgcacttt taggtataac agaattaact acatttaagg agattgatga
     2941 accatgaaaa agaaaaaagg ttttggtctt ggtattagtt taatcgccat catgttaatt
     3001 gtatgtattg tattagtaat catgatgatg actggcggaa agaaagatac atactatgga
     3061 attatgaaag ataatactac tattgaaaaa atgattagtg aaaaagatga aagcattgaa
     3121 aaaaatgtta aattaccttc agattcagat gttaaagtta aaaaaggtga ttttgtaatt
     3181 gtttataaat tagcagattc agataaaatt gttaaagtta aaaaagttga ccatgatgac
     3241 gtccctcacg gtttaatgat gaaaattcat gacatgggca aaatgcacat gaaacactaa
     3301 ttgtaattta aattacaaat tttagttgcc atcaaggtat atacgagtaa aagcagcggt
     3361 aagttgattt ccaatttgga atcatcttac tgctgctttt taagaattac tgtgtcagtc
     3421 tattttcggt tcgcctgcgc ttttgcgcat gcataaaagc ccctaacaac ctgaggtcac
     3481 tgcgcttcgg ttcgcctgcg cttttgcgca tgcataaaag cccctaacaa ccaaaggttg
     3541 taaggggctt taaatgtcag atagtatttt ttaaattgat tttaaatatt cttgaccttt
     3601 tgctttgtca tatacgtttt cccatttagc tataacgatt gttgataatg cgttaccaat
     3661 aacgtttaca catgtacgta ccatatctaa gatacggtca acaccaataa ttaatgctaa
     3721 accttgtgcc ggtaagccca tggcacctaa tgttgttaat aaaacaacga ttgaagttcc
     3781 tggtactgcc gccatacctt tagacgtaat cattaatgtt aacatcaaca caatttgctc
     3841 tgataatgtt aagtgcattc catacatctg tgcaacgaat aatgctgcaa tagattgata
     3901 aagtgctgat ccgtctaagt taaacgtata accaattggt ataacaaaag aagtaatttc
     3961 ttttggagaa ccgaagtttt ccattttctt catcattaca ggaagtacag cttccgaact
     4021 tgatgtagaa aatgctaaaa gcaattcact tttcaagatt ctaataatat tcatgatatt
     4081 aataccacac atccatgcaa ctagtcctag tatagcgaat acaaagaaca ccattgcaaa
     4141 gacaacaact aatactaatt ttaatagtgg taataatgcg gatgcaccaa atgtaataat
     4201 tgtagtacaa atgaatgcaa acactccaag tggtgctaat tttaaaattt tattaatcat
     4261 ccagaacaca gcttcaagcg atccgcttaa aaattcttta actggttctg cttttttacc
     4321 tacagcagct aatcctaatc caaagaatac tgcgaagaag ataataggta ataattcacc
     4381 cttatttaaa gcttcaaaaa agtttgtcgg aataatatgt acaatggtat caataaaatg
     4441 atttccatat gtagattgtt ctgctgcatg tgcagttgat tgatatttag aaatatcacc
     4501 tttaggtaat tttgttgggt ctaatccagc acctggttta aataggttac cgaagataat
     4561 ccctaaacct attgcgattg ttgtaataat ttcaaagtat aaaattgttt tccagccata
     4621 acgccctaca gttttcgatt ccccaacgtt cgaaatagaa agcgctagtg agcaaaatac
     4681 aactggtata acgatcattt taattaaatt taaaaatacg tcaccaaatg gtttaatgta
     4741 atttgctacg ttttcttgtc catataataa aagtcctacg acaacaccta acactaatgc
     4801 aataacaact tgcataggta ggctaatttt tctcttgaat agagccatag tacaaactcc
     4861 ccttcatctg acatcactca attatatcag aaaattgata attatctata aaaaattaaa
     4921 atatttatct
//
LOCUS       DBNENW010000161         4859 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNENW010000161 DBNENW010000000
VERSION     DBNENW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.164"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            76..402
                     /locus_tag="AC1LW8_002340"
     CDS             76..402
                     /locus_tag="AC1LW8_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HIP4774133.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            404..883
                     /gene="rsbW"
                     /locus_tag="AC1LW8_002341"
     CDS             404..883
                     /gene="rsbW"
                     /locus_tag="AC1LW8_002341"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HIP4774134.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKNENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIN"
     gene            858..1628
                     /gene="sigB"
                     /locus_tag="AC1LW8_002342"
     CDS             858..1628
                     /gene="sigB"
                     /locus_tag="AC1LW8_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HIP4774135.1"
                     /translation="MAKESKSTNEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2061..4211
                     /locus_tag="AC1LW8_002343"
     CDS             2061..4211
                     /locus_tag="AC1LW8_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIP4774136.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKNDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4204..4659
                     /locus_tag="AC1LW8_002344"
     CDS             4204..4659
                     /locus_tag="AC1LW8_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HIP4774137.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1852 a          609 c          951 g         1447 t
ORIGIN      
        1 atttgaaata taaatatggt ggatacagcg cttaaaatga agataaatat ttttaataag
       61 taggagtgta atgaaatgaa tcttaatata gaaacaacca ctcaagataa attttacgaa
      121 gttaaagtcg gtggagaatt agatgtttat actgtgcctg aattagaaga ggttttaaca
      181 cctatgagac aagatggaac tcgtgatatt tatgttaatt tagaaaatgt gagttatatg
      241 gattcgacag gtttaggttt attcgtaggt acattaaaag cattaaacca aaatgataaa
      301 gaactataca ttttaggtgt gtcagatcgt atcggtagac tatttgaaat tactggtctt
      361 aaggatttaa tgcatgttaa tgaaggaacg gaggtcgaat aacatgcaat ctaaagaaga
      421 ttttatcgaa atgcgcgtgc cagcatcggc agagtatgta agtttaattc gtttaacact
      481 ttctggcgtt ttttcgagag ctggtgctac atatgatgat attgaagatg ccaagattgc
      541 agttagtgaa gctgtgacaa atgcagttaa acatgcatac aaagaaaata acaatgtagg
      601 cattattaac atatattttg aaattttaga agataaaatt aaaattgtta tttctgataa
      661 aggtgacagt tttgattatg aaacaactaa atcaaaaata ggtccttacg ataaaaacga
      721 aaatatagac tttttacgcg aaggtggcct aggtttattt ttaatcgaat ctttaatgga
      781 tgaagtgaca gtatataaag aatctggtgt gacaatcagt atgactaagt atataaaaaa
      841 agagcaggtg cgaaataatg gcgaaagagt cgaaatcaac taatgaagtt tcacctgagc
      901 aaattaacca atggattaaa gaacaccaag aaaataagaa tacagatgca caggataagt
      961 tagttaaaca ttaccaaaaa ctaattgagt cattggcata taaatattct aaaggacaat
     1021 cacatcacga agatttagtt caagttggta tggttggttt aataggtgcc ataaatagat
     1081 tcgatatgtc ctttgaacgg aagtttgaag cctttttagt acctactgta atcggtgaaa
     1141 tcaaaagata tctacgagat aaaacttgga gtgtacatgt tccgagacgt attaaagaaa
     1201 ttgggccaag aatcaaaaaa gtgagcgatg aactaaccgc tgaattagag cgttcacctt
     1261 ctatcagtga aatagctgat cgattagaag tctcagaaga agaagtgtta gaagcaatgg
     1321 aaatgggaca aagttataat gcgttaagtg ttgatcattc cattgaagct gataaagatg
     1381 gttcaactgt tacgctatta gatattatgg ggcaacaaga tgaccattat gacttaactg
     1441 aaaaacgtat gattttagaa aaaatattac ctatattatc tgatcgcgaa cgagaaatca
     1501 tacaatgtac gtttattgaa ggattgagtc aaaaagagac aggtgagcgt atcggtttaa
     1561 gtcaaatgca tgtatcacga cttcagagaa cggcaattaa gaaattacaa gaagcagcac
     1621 atcaatagaa tttgtttatt aatgatacgt tttataatga aaaatccata taattatccc
     1681 ttgattatta aattgaaatc gaggggtatt tttaatttaa ttaagatttt cgaattaata
     1741 cattattagt gtagtttaat gtgtatccac ataaatgtcg cgatatagta ttaataattt
     1801 aagtgaagaa gatatctaat tgtcgtttta aataggtggg ttgctattag aataaaaaag
     1861 tagtcttaga ttatgaaatt tagaaatgat ggtgtgtcat tttcaataat cttagtgcgt
     1921 tttaaaatat agtatgacct aatcattcgt tttaaatgtt ttggaagtga aaattacatt
     1981 aagtatcata ccttaataga agtattttag aatatgttaa aataaatgag taaatttaag
     2041 aaaaagtgtg ggttaagtaa atggacaatc aattgattaa ttcaatcata gagaaatatc
     2101 aatttagtaa aaaacaaatt gaagcagtat taacactgct agaagaaaaa aatacagtac
     2161 catttattgc gaggtatcga aaagagcaaa ctggtggact agatgaagtt caaataaagc
     2221 aaattgatga cgaataccaa tatatggtca atttacaaaa acgtaaagaa gaagttatca
     2281 aaaatataga acagcaagga ttacttactg aggaattaaa gaacgatatt ttaaaacaga
     2341 acaaattaca acgtgttgaa gacctatata ggccttttaa acaaaagaaa aagacaaggg
     2401 caactgaggc gaaacgtaaa gggttagagc cattagcgat atggatgaag gcacgtaaac
     2461 atgaagtctc aattgaagaa aaagcacaac aatttataaa tgaagaagtg caatcggttg
     2521 aagatgctat caaaggtgca caagatatta ttgcggaaca aatttcagat aatcctaaat
     2581 atagaacaaa aattttaaaa gatatgtatc atcaaggtgt gttaactaca tctaaaaaga
     2641 aaaatgctga agatgaaaaa ggtatttttg aaatgtacta tgcatatagt gagccaatta
     2701 aacgcattgc taatcataga gttttagctg ttaatcgtgg tgaaaaagag aaagtattat
     2761 ctgtaaagtt tgaattcgat acgacatcag tagaggattt cattgcacgt caagaaatca
     2821 atcataataa tgtaaatcgc agttatattt tagaggcgat taaagatagc ttgaaacgct
     2881 taattgtccc ttcgatagag cgtgaaatcc atgctgattt aactgaaaaa gctgaaaatc
     2941 atgcaataga tgtttttagt gaaaacttaa gaaatctatt actgcaacct ccaatgaaag
     3001 gtaaacaaat attaggcgta gatccagcat ttagaacagg ttgtaaatta gcagtcatta
     3061 acccattcgg tacttttata gcaaaaggtg tgatttatcc gcatccacca gtttctaaaa
     3121 aagaggcagc agagaaggat tttgtacaaa tggttaaagc gtatgatgtg caattaattg
     3181 caattggcaa tggtactgca agtcgtgaaa cagaacaatt tgttgcagat ttaattaaaa
     3241 agcatcagtt gccagtacaa ttcatcattg tcaatgaagc gggcgcttca gtatactcag
     3301 catcagaaat tgctagagat gaatttcctg attttcaagt ggaagaaaga agtgccgtat
     3361 caattggacg acgcgttcaa gatccattaa gtgaattagt taaaatcgat cctaaatcta
     3421 ttggtgtcgg acaataccag catgatgtta atcaaaaagc actagaaaat gcattaactt
     3481 tcgttgttga aacagcagtt aaccaagtag gtgttgatgt caatacagca tcatcatcat
     3541 tattacaata tgtgtctggt ctaagttcgc aaattgctaa aaatataatt gcatatagag
     3601 aagaaaatgg cgcaataaag cataataaag agttaagcaa gattaagcgt ttgggtgcta
     3661 aaacttttga gcagagtata ggatttttaa gaatagtaga cggttcagag ccattggata
     3721 atacatcgat tcatcccgaa agctataaag taacgtatca gttattagat aaactcggat
     3781 ttggtggcaa tgatttaggt agtgatgcat taaaagctaa attaaattca ttggatatgg
     3841 atgaactggc gattgaatta caagtcggtg tacctacatt agaggatatt attaaatcct
     3901 tgaaagcacc taatcgagat ccaagggacg agtttgaaac accaatcctt aaatccgatg
     3961 tgctatcaat tgaagattta caagaaggaa tgaaattgag tggtacagtt agaaatgtag
     4021 tagattttgg agcatttgta gatataggtg ttaaacaaga tggacttgtc catgtttcga
     4081 aactttctaa gaagttcgta aaaaatccaa tggatattgt cagtgttggt gatatcgttg
     4141 acgtttggat ttacagtatt gacaaaaata aagacaaagt atcattaacg atgattgatc
     4201 cacatgaata atgacaaact tcagcgaatg gttgagaatc tttcggaaga aaaatttgga
     4261 cgtacgtttc gacattgtgc atattttaat aaaaggttac gtacaacagg cggacgttat
     4321 cttttaaagt cccatgatat tgaaattaat ccaaaacaat atgagcatta tggagaagat
     4381 gctgttgtaa aaattatact gcacgaatta tgtcattatc acttgcatat tgctggtaaa
     4441 ggttatcaac ataaagatca agattttaaa cgattgagcc aacaggttgg agcgcctaga
     4501 ttttgtaaca gcattgaaag ctatcaacaa cgggcaaatt atgaatatta ttgcactaaa
     4561 tgtcatgcaa aatatatacg tatccgtaaa gttgatacga atcgtatgcg ttgcggacat
     4621 tgcaatggaa aattaagaat gaaaagacaa ttgaaataag tacaatacaa atagagctaa
     4681 ttcgtgtata tgtaagatta tttatgagtt gtatggaatt tatatagaat ttatcagtga
     4741 gttattttga ttggtaaaca tagtaattga ataagaagaa attgtgttta taaaatgttt
     4801 aaagccgaga ttgtcgtaat ttaatacttc gcaatctcgg cttttctact tatgatata
//
LOCUS       DBNENW010000162         4823 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNENW010000162 DBNENW010000000
VERSION     DBNENW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.165"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..421)
                     /locus_tag="AC1LW8_002345"
     CDS             complement(<1..421)
                     /locus_tag="AC1LW8_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HIP4774138.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFITYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTILFPQYGHYFMFLL"
     gene            complement(1154..1519)
                     /locus_tag="AC1LW8_002346"
     CDS             complement(1154..1519)
                     /locus_tag="AC1LW8_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIP4774139.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(1661..2083)
                     /locus_tag="AC1LW8_002347"
     CDS             complement(1661..2083)
                     /locus_tag="AC1LW8_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIP4774140.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            2217..2957
                     /gene="ecsA"
                     /locus_tag="AC1LW8_002348"
     CDS             2217..2957
                     /gene="ecsA"
                     /locus_tag="AC1LW8_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein EcsA"
                     /protein_id="HIP4774141.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHKQTLDDIYIHVTQGGDVHA"
     gene            2950..4173
                     /gene="ecsB"
                     /locus_tag="AC1LW8_002349"
     CDS             2950..4173
                     /gene="ecsB"
                     /locus_tag="AC1LW8_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease EcsB"
                     /protein_id="HIP4774142.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(4297..4800)
                     /gene="traP"
                     /locus_tag="AC1LW8_002350"
     CDS             complement(4297..4800)
                     /gene="traP"
                     /locus_tag="AC1LW8_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HIP4774143.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPTKYDVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
BASE COUNT         1646 a          697 c          806 g         1674 t
ORIGIN      
        1 ccaataagaa cataaaataa tgtccatatt gcgggaataa aattgttaga taaacgaaca
       61 ttgcagataa gataattaac ggcaatatta agttaatgca aaaataaaat ttgtgtacag
      121 gttgattgac gtaggtatta tagaatataa gccattttct tttagtcaac ttatgaactt
      181 taaacgattt tctatatggt aagaaaaata acaaatgtat acttttatgt aatggatacg
      241 ttataaatac aagtaataaa aagatgagaa aatgtctatc tgataatggc gtattagata
      301 aaaaatacat tacttcataa ctaactaaaa acataatgat ggttgcaact gcactcataa
      361 atgcaattct aggcaaacca aatcgtgcat tgatatcaat ttgtctttta cataaaaaca
      421 tatcttacgc ctcatctctt ttcaatataa gttagattat aactaactca ggttcagtaa
      481 ttcaatagta acatatcatg ttagaagaat tgttatataa gaagaaaggg gcgaagtatt
      541 gtttgatttg aaacgcgaaa gtggagtttg gtaaaattgg ctgattgact agcgtagaag
      601 tagaatgcag aacaaatttc tgcgaacaca tcttcttgct aataaagcat cacttatgaa
      661 aaaggcgcgt gtgacatgag tatttgaaaa cttgaataat ataaaaaatg gatatggcag
      721 ggcatatgca aaagcatctt ttgaccaact aagttataca aatgctaaaa atatagtgat
      781 atgtaattat aataaatacc agattaataa tttgtgcaag taaggtatat gtaatgtgca
      841 taaaaggttc ggctcaatga aaatataagt atgttgtttg tcgataatga tttaagtaaa
      901 gcgtaatcaa agtgcaagga taacacaatg aatcctaagc aatgttttca atcaattcat
      961 aaatatgatt taagcgaaaa tcactacaaa cgatgtattc ctgtaatttg taaacataag
     1021 ttcatttgag ttataaaaaa gagatgacaa gaaatatcta atttctaatg tcatctcgtt
     1081 tatttggcat ttaattaaaa aaaataaata tgctgttact aataataata gttgtgatcg
     1141 agaacgtaac gtgctactta gaaatttcgt ttccaatatc ctcgccacga ttttgtaaat
     1201 tttcgatgtg tgactgtaaa cgttcaatat taggattaat gtcagatttg aagtttccaa
     1261 gtaatgactt aatttcacca ccaattgaac taccaaattc cgcactttcg tttttaattt
     1321 gaacaccata gtttaaaatg tcattaaaac tttgtttaat cgtttcaaat tcacgttgta
     1381 gttctgattt tgaaccagta ggggcagtac gatcgatcgt gttgttcttg acacttttgt
     1441 cgtctcgtcc ttgaagtgca actacaaaac cagctgctac gccaacaccg ataccgaata
     1501 gaatgcgtga tgctttcatt tatattctcc tcacttttaa catctgttat cttgataact
     1561 cattttgttt aaaattgcta accttgatgt tatcacttca aacgcaattg ttataatatt
     1621 aatcatatta tacccgttcg agcttagatt aagcatacat ttaaaattgt gcttgaattt
     1681 gttcagcaat ttgttgtttt gcatcgttat ctaaaatgtc ttcatgtgtt tcccacttat
     1741 aaccaaatcc atcaatatca ttttcatatc gaggaattaa gtggaaatga atatgaaata
     1801 cagattgatc tgcaaactca ccattatttt gaataatgtt taaaccatca ggattaaatg
     1861 cacgcttaat agcatttgct actttaggta atgctgcacc gatatgtttc attgtttctt
     1921 catcagtttc aaagatatta gcagaagctt ttttaggaat taataacgta tgtcctttag
     1981 taacttgtga tatatctaaa aaggcataga cataatcgtc ttcatatact ttaaagctag
     2041 gaatttctcc agttaaaatt ttgccgaaaa ttgtttctga catgatgact acactccttt
     2101 tcaacttgta ttaccattca ttataacatt gtcgctaaaa attcgatatt aggacatgtg
     2161 tatttaatat gagcatttta taatttttgg tacaatataa gtcatggagg tgccttatga
     2221 cagttaaagt agaacagctt acaggtggat atggaaaacg ccctgtaatt aaagatataa
     2281 attttgaatt aaacaaaggc gaaatcgttg gacttattgg ccttaatggc gctggtaaga
     2341 gtacaacgat taaacatatg ctaggattgc ttacacctat ggaaggatct ctatcaattt
     2401 cagatatcaa cattaatgat gacatagagg cttatagaag aaagttatct tatattccgg
     2461 aatcaccggt tatttatgaa gaactcacat tagaggaaca cattgagatg acagcaatgg
     2521 catatgatat tgatcgtgat gaagcgatga atcgagcaat gccattatta aagacattcc
     2581 gtttagaaaa tgaattgaaa gtttttccga gtcatttttc taaggggatg aaacaaaaag
     2641 tcatgattat ttgtgcgttt atcgttaatc ccgaattgta tattatagac gaaccattcc
     2701 ttggcctaga tccattagga attcaatcca tgttagattt aatggttgaa aagaaaaacg
     2761 aaggtagaac cgttctaatg agtacacata ttttagcaac agctgaacgt tattgtgatc
     2821 gttttatcat actagacgaa ggtgaagtcg ttgcatttgg agatttagaa gcattgagac
     2881 aacaaactgg cttacataaa caaacattag acgatatata tattcatgtg acgcaaggtg
     2941 gagatgtaca tgcataacca agcgaccaca ttatttaata aaagattaca tgcccttcgt
     3001 aaagagaaaa attattataa taagtttata tttaatggtc atttcatggt ctttttactt
     3061 attttgctgg gtgcatttat ttttggatat ggtgaatggt taaaacatat tccaacgaat
     3121 atcaattttt cattaatagc agcagtgatt gtagcattga catccatttt tccaatgcga
     3181 ccactcctaa aagaagcgga taaaatcttt ttattaccat ttgaaaaaca catgtctcaa
     3241 tttatgcgtc atgcgatttt atatagttac tttgcgcgca tattaatcca actcattatt
     3301 gttattgtaa tgttcccgtt gttttacaat attaatcaac ataatgtggc attttatatt
     3361 tgttttggag tcagtgcatt aatttttcca tatgttggtt tacgcttaag atggcaatgg
     3421 tatcaatcgg gacttaaaac atggcaagtt aatttaatat cattcatcac ctttgctcta
     3481 acatattact tactattagc tccaaaatgg tacatcgcat ttgtgatggt tgctttgcct
     3541 gtgttaatag aatttttagt taaaaaatat aaaccaggct ttttgtatcc ttgggagaaa
     3601 atgattgcaa tcgaacatcg acatcatatg aattattata aatttgttaa tatgttcacc
     3661 gatgtaaaac atttgaaaga atctgcagtc agaagaagtt atctagatat acttttacca
     3721 gttccaaagg gtagtaaatt taactctaat gcgatgtatt tattcttatt tattagaagc
     3781 tttataagag ggagagacgc atttaatatt atctttaggt tagtaattat tgcagtttta
     3841 ttaatggttt ggttatcata tccattagtg acagcaatta ttgggtgttt atttgtatat
     3901 attatattgt tacaaatggc acaattttat tcacagcaag catatggctt atggcctcaa
     3961 gtgtggcctg tacctgaaga gaaggtaatc aaagggtatg aacagttttt atatagatta
     4021 atgtttgtta tttgtactgt gtttgcagtg acatttatta taaaacatat gacgttattc
     4081 tatgttgtac tcattttcta tattgtaggt ttattaacga ttagaagtat tattaaaaag
     4141 ttgaaatatc aggaaacatt attacgagac taaaaaactt ccattggcat gtatgttgta
     4201 aaggtgcatg taatgttgaa cgccaaatga tacggcgttc agattacatt agcatctata
     4261 cgttaacagc ataaccaatg gaagttttct tcgaatctat tcttttattg ggtatagata
     4321 tctttcaaaa taacttgaat gttgtccgct tgaaccaaag taatgactta atgcgtcttt
     4381 tgaaaaatgg tcattaaagg catcagattg cttaaagtct tcgtatgcat gtcgatcagc
     4441 aaatccaaaa taaattttgt atgttgtacc tttagcaggt cttaacaaac gatagctttt
     4501 aaagccacca aagtttctga aattatcgtc tacactaatc agtttctttt caagttgata
     4561 tgcatgatct tctgttgatg gaatgaagat tgcacaatag aaatgatgtt cactgaattc
     4621 accaatttct ttgataacat catattttgt aggtgatttt aaaacagtct caccatcagt
     4681 ttcttcaaaa ataactgaag tatctgatgc tgaaaattgg aatagttgat gggtcgggtt
     4741 attgattttt atctgatgta aaaatccata agtgccataa gatgtatata gtttcttcat
     4801 atgaaaggtc ccccttatgt ttt
//
LOCUS       DBNENW010000163         4739 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNENW010000163 DBNENW010000000
VERSION     DBNENW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.166"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            1..334
                     /locus_tag="AC1LW8_002351"
                     /pseudo
     CDS             1..334
                     /locus_tag="AC1LW8_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669720.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(658..1944)
                     /locus_tag="AC1LW8_002352"
     CDS             complement(658..1944)
                     /locus_tag="AC1LW8_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4774144.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNVTIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPDHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2144..2242
                     /locus_tag="AC1LW8_002353"
     CDS             2144..2242
                     /locus_tag="AC1LW8_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp-rich small protein"
                     /protein_id="HIP4774145.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2485..2661
                     /locus_tag="AC1LW8_002354"
     CDS             2485..2661
                     /locus_tag="AC1LW8_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1626 family protein"
                     /protein_id="HIP4774146.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            2920..3300
                     /gene="pmtR"
                     /locus_tag="AC1LW8_002355"
     CDS             2920..3300
                     /gene="pmtR"
                     /locus_tag="AC1LW8_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSM export ABC transporter transcriptional
                     regulator PmtR"
                     /protein_id="HIP4774147.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3297..4193
                     /gene="pmtA"
                     /locus_tag="AC1LW8_002356"
     CDS             3297..4193
                     /gene="pmtA"
                     /locus_tag="AC1LW8_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HIP4774148.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNIITPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            4194..>4739
                     /gene="pmtB"
                     /locus_tag="AC1LW8_002357"
     CDS             4194..>4739
                     /gene="pmtB"
                     /locus_tag="AC1LW8_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HIP4774149.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFLLSQ"
BASE COUNT         1639 a          639 c          800 g         1661 t
ORIGIN      
        1 aaacaataca ctaaatcaaa aaaaaataaa taaatctaat aatgtgaaat tcccagtaac
       61 aataaataaa tttgaaaaca tagtttcaaa tgaatttgtg ttctataatg caagcaaaat
      121 tacaattaat gatttaagta taaaacttaa atcagcaatt gcaaatgatc aagggataac
      181 taaacatgac atagaacttg ctgaacgcgc agtgtataaa gtgtatttta aaaatggttc
      241 gtcaaaatat gtagacttaa aaactgagta taaagatgaa agagtattta aagcaactga
      301 cattaaaaag gtagatattg aacttaaatt ctaatcttta attttataat aaggcatctc
      361 acaatcgtgg ggtgcctttt acatttgtag agatgtgatg cttgaatgtt tgaagtgatt
      421 tgccaaaagt tatataaatt ggttctataa taaatcggac tgatagaaaa agttttttac
      481 ttttcatctg tccgattttt tgattttgtt aggagagatg tataaaacta attaggtata
      541 gtgtttgaca agttgctgaa taaaatatat cttatgatac tttgaaagaa ggaataattt
      601 taaaaataaa aaaccaataa tccgagtcat actcatcaga ttattggttg aaattaatta
      661 tcttaagtca tcaatcgctt tagcaattga atcaaataca tgtgggatat cttctttttc
      721 tacacagcta aacgcaatac gaatatctgt tgcattaagt gcaataacac caattgaata
      781 tttttcaatt aaatgtttac gaagttcttc agggtcaaca tcatgaactt ttattgccat
      841 aaagtatcca gagttaaagt cataggcttg ccaatgtgaa tgatattgat cagcatagac
      901 aacttcctta gtgacttcat agcgttcttt taatgtttga atattttgtt cgatttcttt
      961 atcgaattgt ttattatttt ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc
     1021 actagaaatg ttacttcgta taagaccttt tactttggct tctaatactt ctttagttgt
     1081 ttgatcagac gttccaaatg tcataaaccc aacacggaat ccccatgcga aaaattcttt
     1141 tgttgcacca tctaaacgaa caggtaatat tgcatttgaa tttaaattag ataatgcagt
     1201 aaataatgat tgagtataca catcttcata gaataaacca tagtatgcgt catccacaac
     1261 agctataact tttgtacctt tatcagctaa tgctttaatt gcatcgacga tggtagtcac
     1321 ttctttatgc gtaggtgtgt aacctgtcgg attattagga taatttaaaa tcataatgac
     1381 tttatcttta ttgtatgatt gtaaagcttc tacaagtgaa tcagtagtat aatgcccgtc
     1441 tttatcaaag ataggatatg tttgaaggtt tgcaccattt ctcgtattga aaacaagttt
     1501 gtaattaccc caattatgat ctggtaacaa gatagtgtca ccttgattta caaataaatc
     1561 gccaactaaa gataaaccat gtgttaatgc attcgtaaca attggtagtg acatgttgtc
     1621 gattgatagc tctggattgt cacgcaacat tttttgttgc cataaatcac gtaattcttc
     1681 gatgccttgt ggtggcgcat aagggaatat ttcatctgga gttaaatcat taaacattgc
     1741 atctaaagat gatgcaaaca tttttccgtc tttgtttgtc gccataccta tagttacatt
     1801 atatgttgta ctctttgctt cagcagattg agataaaatg ccttttggat aaaacatatt
     1861 ttgacctaag tcagataaca ttgcaaaggc agttgcattt gactgttgaa gttgttcatt
     1921 taagctttgg gctaaaggat tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct
     1981 atatattatc ttcaattata ccaaagataa agtgttgttg gtgaattaac gaataatatg
     2041 taaaatctgc tgccaaaata cgaacatatg ttctgttttt atgttagaat aggtgtaggt
     2101 catccttcag tatgatactt tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga
     2161 agggatgatg acacttatta caggtggctt actaattatt tttcgacttt ggttagaact
     2221 gaaatggaaa aataaaaaat gatcctacac acataaataa ccccaagtct gctggtacca
     2281 gacttggggt tgtgtagtat aattctgtgg atacacttaa ataatatcat tgaacacaat
     2341 atatcgcaat caataatcat tttgattgaa tggttttgtt gcgctatttt agttgaaagt
     2401 gattagtacc ttgaactttt gaaattcaat aacttattaa aaaaatttat atttgttata
     2461 atgtcgatgt tgaggtgaaa taaattgaaa catctaacaa aaatatttgt agtacttgca
     2521 attattttat ttattattgg ttattattta caagcaacta atcatgaaag ccaaggtata
     2581 aaattattat tagcagcgat tatgtttatg atttgtgctt ttataagtag aagtaatgat
     2641 cgacgtaaaa atagtaaata gaattgactg tttaaaacga ttgtttatgt tgacgctacg
     2701 gttgtcacgt agagattaaa gacattcgtt ttagcagtct tttttattga agtagacaat
     2761 gcaagattaa atggtagtgt catttcattt tattcaactg ctagtaatac aatactcagt
     2821 gttatttctg aagactggtt gataatgaga catgcatatt tacaaaactg tgtatattgt
     2881 gtatattgta tatatacaga aagtgatagg gggacgttga tgaaaataat tttaaaaaac
     2941 aatagtgatt ttccgattta tgaacagatt aagcaacaag taaaacaaaa tattttaaag
     3001 ggacatgttg ctcctggaga gcatttgccg tcaatgagag aacttgccaa agatcttcaa
     3061 gtaagtttga ttactaccaa acgtgcttat gaagatttag agaaagacgg ttttgttaca
     3121 acaattagag gaaaagggac ctttgttaag gagcaagata gttctatttt aaaagagaaa
     3181 caatttttta ccattgaaaa tttggttaaa gaattggtta atgaagcgca agccatcgaa
     3241 atgtcacttg aggaacttca agatatttta acgttcattt atgaggagga atcatcatga
     3301 atgccataga attaagtaat gttaattatt caagtgatca gtttaattta aagaatatct
     3361 ctttcaaagt tcctcaagga ttcgtcactg gttttattgg tagaaatggc gctggtaaaa
     3421 ccacaataat aaggttaatt atggatttgt atcaaccaca aactggtgtt attagagtat
     3481 tagaagagga tatggctctc aatccgatag agttaaagaa tagaattggg tttgtttact
     3541 ctgaaaatta ttttaatgag agatggacta cgaagcaact tgaaaaaatg attgcccctt
     3601 tttatcgtaa atgggaccat caagtatttg aattttatct tgaaaagttc gatttaccaa
     3661 tcaataaatc aataaaaaca ttttcgactg ggatgaaaat gaaattatca ctagcagttg
     3721 cattttcaca tcatgctgaa ttgtatattt ttgatgagcc tacttcaggt cttgatccct
     3781 tagctagaaa tgagctattg gagataattc agcaagaatt aattgatgaa aataaaacaa
     3841 tattcatgtc gacacacatt atctcagatt tagaaaaaat agctgattat atcattcact
     3901 taagtgacgg tgaagttatc ttaaatggtt caaaagaaca actgttacaa agatatcaag
     3961 ttgtgagtgg tgctattgag gaccttgatg atgaattagc atctctactt atttatgaag
     4021 aacataagcg tacgggattt ataggattaa cagaacatgc gcaagtattt aaagaaattt
     4081 taggtcataa agtgaatatc attacacctt ctattgagaa cttaatggtt tatcttgaaa
     4141 agagaaaacc taaatatcat gaaaacatca aattgatgga agaaggtttt taaatgaagg
     4201 cattgttaat taggaatttt aaattacgac gatatacact tatcatctat gttctattat
     4261 taaccttata tcctttttat ataatgcttg actcaacaaa gttcttttac ctattgcaat
     4321 cttttataag tccaacaata ttgattattt ggattttaga tgctggtcac ttatttagac
     4381 tcaatcgtag attaggtggt aatgattcat attattttta tatgagttta ccagtgtcaa
     4441 aaaaacaatt gcttaatgcc aactatatta catgcattgt tttaacatta attggaacgc
     4501 ttgttattag tttatatgct tatgaagcag atgtgattga accaaattca atctattttt
     4561 caactgcata tgcatttgtc atatctaact tcttgtctat accaattgca tttagtcaat
     4621 ttacagaatt gcgtagagtc aaagtgccat atggtatata cgtgtttact attatcattt
     4681 tagttccatt tttattttca attgcaatag tgttggtgaa ttattttctt ctaagtcaa
//
LOCUS       DBNENW010000164         4671 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNENW010000164 DBNENW010000000
VERSION     DBNENW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.167"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(57..578)
                     /locus_tag="AC1LW8_002358"
     CDS             complement(57..578)
                     /locus_tag="AC1LW8_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HIP4774150.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(606..1901)
                     /locus_tag="AC1LW8_002359"
     CDS             complement(606..1901)
                     /locus_tag="AC1LW8_002359"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP4774151.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(1941..2417)
                     /locus_tag="AC1LW8_002360"
     CDS             complement(1941..2417)
                     /locus_tag="AC1LW8_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP4774152.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2586..3121)
                     /locus_tag="AC1LW8_002361"
                     /pseudo
     CDS             complement(2586..3121)
                     /locus_tag="AC1LW8_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            complement(3332..4270)
                     /locus_tag="AC1LW8_002362"
     CDS             complement(3332..4270)
                     /locus_tag="AC1LW8_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4774153.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(4540..>4671)
                     /locus_tag="AC1LW8_002363"
     CDS             complement(4540..>4671)
                     /locus_tag="AC1LW8_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774154.1"
                     /translation="ALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         1483 a          880 c          630 g         1678 t
ORIGIN      
        1 attgagaaat gaatcatatc tttatagatt aattgggaaa ttttagtttc taatatttaa
       61 ttaaatgctt gatgtaaagc atcagaagaa tgcttccaca taccttcatc atggttcatt
      121 aaaaagtctt ttaaaatagc tttattctcg tcaccaattt gatcaatgat aatatgatgt
      181 ttcattgatt tatccatcat attaacatgt tcaggcatac ttttataacc acgacgaatt
      241 gctttattca ctaataaata gcacgctgtt aaaccagcgt aatatggtcc ttgttcatta
      301 cgttctgttg ttacccaaca caaccaatat tctttagctt catcgccttc aacagcagct
      361 ttatcagtaa tccatttcac acctttttca actttggcac gagcatgcat gcctccaata
      421 tcaataaatg cttctttgtt agcaacatcg ataaaaactg gtgcaatatt gtctaagcta
      481 attgaaccaa tatttgtacc tttatgacca tctagagggt cattttttat gatattaaaa
      541 ttaaagcctt ttttctcaga caatgatata acctccatat ttaacgataa caattttagc
      601 cctgtttaat taagctcatt aatcaaattt tcaatagctt ctctaattgt cttatcttta
      661 atctttgcaa ccatttctct aggataatat aatttatagt cttggcgatt aatgcctgta
      721 atactatgaa tgactaatga ttgattgctt atttctctaa tcgtaccatc ttgtcttaat
      781 aaatgaatcg gtttgcgatt tgaccccggt cgatcatagt catatggcaa atccgaaaat
      841 gcttcactca caaaataata atctggatta ataccacctg cttcaaacag ttcttgcagt
      901 tctgatattg taataattga gccatcaaat ggaatatatt taaataagtc tcgattaata
      961 aatcgacttg ctaaatcact taaaatggca tcatcttctt ttgtccattt ttccaaataa
     1021 tacgtaacta ccgcttcatc gagttcaaca tattgttcaa tcgtaactgt ctcttcaaaa
     1081 aatggaataa aatcatgtgg atgcaactta aattcatagc cttcattata aagctgtttt
     1141 gcgcgtttca aacaattatt aagcagcact tctccaccac gactaactgg gtggaagtaa
     1201 atttgccaat acatttgata acgactcata ataaagtttt caactgcatg cataccactt
     1261 tctttgatta gtacttcatc tttagaaggt cgcattaatc ttaaaatacg ctccatatca
     1321 aaagcaccat atgatacacc tgtgaaatac gcatcacgtt gtaaataatc cattctatcc
     1381 gcatcgattt gtgacgaaat catcgaaatg accaatttat tatgatgcgt tttattaatt
     1441 acttccgcaa cttctcttgg aaactcaggc gccactttac gtaatacagc attcacctca
     1501 gtatctccag taataatcgc ttgtgtgtat gcttcatggt ctgtattaaa tattttttca
     1561 aaactatgtg aaaatggacc atgtcctaaa tcatgtaata atgcagcaca taatgccaac
     1621 ggtctatctt tattgtccca tgcatcatga ccaataaatg actcatcaat taatcgtcta
     1681 actatttcat acacacctaa agaatgtcca aagcgactat gttctgctgt gtgaaaagat
     1741 aggtacagtg ttcctagttg tctaattcga cgtaaccttt ggaattcctt agttttaatt
     1801 aaatcccata tcaattgatc ttcaacatga atatatcgat gtatcgggtc tttaaaaact
     1861 ttttcttcta ctaattttaa atctacatat gcgttagtca ttattcccct ccttttcgtt
     1921 taatataata tttaatttac ttaaaatgct ttgtacataa gtgctaagtc taacttttcg
     1981 ccatacattt ctggctcata agagcgtaag attgtaaaac cttgctcttt atagtaagct
     2041 actgcttctt catttttatt atctacttct aagtaaacac cttcaaattt atcttcaaaa
     2101 cgtgataatc cttcatttaa caatgctgta ccataacctg tatgttgcga ttctggttta
     2161 acataatgag ctgataaata taattcttca ccgtaaataa agttagcaaa gccaacgatg
     2221 tcattacctt cttcaacgac taagaataat tgttcttgaa gtcttttctt taaatgatgt
     2281 tcattatatg aagcttctaa caagtgatta actgttgtcg cagcgtatat atttaagtat
     2341 gtattaaacc aagctttagt tgcgacatct ctaatttgaa caacatcttt ttcagttgct
     2401 tgtcttacct tgaacatgac tttctcccct tattaacaag ttttaataac ggcattatac
     2461 cacaacttgc tcaatactta ataaacaatg attgtctatt caatttatat atttatattt
     2521 tccgttaaaa ttaaaaataa aaaataacga agcaaaaaat cacttcgttt agtatgaggt
     2581 atgtcttatt gcaatatact attccactca gttgcacgtg ctaaggcata gttgtctttc
     2641 atgatgtcac caggcttttc agcagttcca ataatataac catttaaagt ggcacctaaa
     2701 agtctaaact atatttcatt tgcgtaattg ctggcttcgc ttttattttt ggacaatctc
     2761 caccaactaa aataactcta aaatcctttt cggccatttg tgccttaaaa ttaggatatc
     2821 gtttatcttg taatgtttct gaccaatgtt cgataaatgc tttcaatggt gctgaaatgc
     2881 tataccaata cactggtgat gcaaaaataa ttgtatcact agccaatatt ttatctagaa
     2941 tcggcaaata gtcatcgtca tatgaagtaa tagtctctgc tgtatgtctc acgtcacgta
     3001 tcggtttaaa ctgatgttgt gtcacgtcaa tccattgata ctctaaatct tgcaaagcga
     3061 attttgttaa ttgtgcagta ttaccgtttg gtctactccc accaaacaaa acagtaatca
     3121 ttttagccta acctcacttt tgattaataa atatctgtgt ttttcgttac ctaattatac
     3181 tatcataagc tttgcctacc gaatagtaaa acgcttacaa cttttatata aatttgacga
     3241 aatttcgtca tgccttatat aacgtcgttt gtgatacggg gctaattcat gatgaaatta
     3301 gatacatata tcaccattaa atacaattca tttagtcttc aatcggaaac agttcatcga
     3361 tatattgaat ctcatcatct gataaaacga tatctgcagc tttaatattt tcaacgactt
     3421 gttctgcacg ttttgcacca ggaataatca catcgatagc tggtctcgtt aaataaaatg
     3481 ctaatacaat gttcgcaatt gaagtttgat gtgctgcagc tatgctttcc aaagctttta
     3541 cgcgacgcac attttcttca aatacacctg gtttaaaatc acgacgtgta gtacgatggt
     3601 cactaaattt agtgttctca tcatattttc cagctaaaat accggatgct aatgggaaat
     3661 atggaataaa tgtgatttgg tgatcaacac aatattgtaa tactgcctca ttttcgcgat
     3721 gcaataaatt atattctaac tgtacaacat caacgtaacc atctttattt gcttctttaa
     3781 gttgatctaa tgtgaaattt gatacaccaa ttgctttaat cttcccttgt tccttaagct
     3841 cttgtaatgc tgcaactgct tgatctttcg gagtgttgtt atccggaaaa tgaatataat
     3901 ataaatcgat ataatcagtt tgtagacgtt tcaaactatt ctcaacttgt tgttttaaat
     3961 attccggttg attgttctga tgtacttctt gattttcatc aaattcatga gaccctttcg
     4021 tagcaatttt aatttgctct cgcggatatt ctttaacaac ttctccaacc aattcttctg
     4081 atcgttctgg cccataaata tatgccgtat ctaataaatt aataccatga ttaatggctt
     4141 gacgaacaac atcttttcct tgttcttcat ctaagttcgg atataaatta tgcccaccta
     4201 ctgcgttcgt cccaagtgcg attggaaaca cttcaacatc agatttacct aagtttacaa
     4261 attgcttcat tagacccagc tccttatctt tataactttt ccactatcac actatcatga
     4321 tttaatacaa cttccaaatg ttagaattag agcttgaatt taagcggtgt aataggaaaa
     4381 cgttatataa aactcagtta aaataagtat tgcttaaatt gatatgcata tagcgcgatt
     4441 gctcgttgac ctttaaatta catgtacttt tgaatcagaa attgaaaaac atttaagaac
     4501 gacaatatta aagccattgc aaatttaatt tggtaatttc tattggtttt taagtatgac
     4561 atgaatgttt ttatctataa ttaaagctat attaactaga aaaaaaccca ttataactgc
     4621 aatccaaaca aaatatacta ttatgttttg ataactcatt gataatagtg c
//
LOCUS       DBNENW010000165         4588 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNENW010000165 DBNENW010000000
VERSION     DBNENW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.168"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            210..1220
                     /gene="adhP"
                     /locus_tag="AC1LW8_002364"
     CDS             210..1220
                     /gene="adhP"
                     /locus_tag="AC1LW8_002364"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HIP4774155.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            1297..1404
                     /locus_tag="AC1LW8_002365"
     CDS             1297..1404
                     /locus_tag="AC1LW8_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774156.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            1497..1913
                     /locus_tag="AC1LW8_002366"
     CDS             1497..1913
                     /locus_tag="AC1LW8_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HIP4774157.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLDVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            1910..3571
                     /gene="argS"
                     /locus_tag="AC1LW8_002367"
     CDS             1910..3571
                     /gene="argS"
                     /locus_tag="AC1LW8_002367"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIP4774158.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            3962..>4588
                     /locus_tag="AC1LW8_002368"
     CDS             3962..>4588
                     /locus_tag="AC1LW8_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIP4774159.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQVKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYF"
BASE COUNT         1653 a          595 c          918 g         1422 t
ORIGIN      
        1 tcgattatgt catttctttt aacatattaa ctagggaaac gttaaaagtt aacggttgat
       61 atctaactaa aaacaaggtc acagtagtat gttttaatct ggcgtctatt acaaataaaa
      121 attacatcta taattattcg ttttcttttt tgaaagtaat cgccaattaa tatcatacat
      181 actggagtga ctataaggag gacattatta tgagagcagc agttgtaacg aaagatcaca
      241 aagtaagtat tgaggacaaa aagttaagag ctttaaaacc tggtgaagcg ttggtacaaa
      301 cggaatattg tggcgtttgt cataccgatt tacatgttaa gaatgctgat tttggtgatg
      361 ttacaggcgt tactttaggt catgaaggta ttggtaaagt catcgaagtt gcggaagatg
      421 tagaatcatt aaaaattgga gaccgtgtgt ctatcgcttg gatgttcgaa agctgtggaa
      481 gatgtgaata ttgtacaaca ggtcgtgaaa cactttgccg tagtgtgaaa aatgctggtt
      541 atacagtaga tggtgcaatg gctgaacaag ttattgttac tgcagactat gctgtgaaag
      601 tacctgaaaa attagatcca gcagcagcgt cttctattac atgcgcaggt gtgacaactt
      661 ataaagctgt aaaagtaagt aatgtaaaac ctggtcaatg gttaggtgtt tttggtatag
      721 gtggtttagg taacctagct ttacaatatg ctaaaaacgt tatgggggct aaaattgttg
      781 ccttcgacat taatgatgat aaattagcat tcgcgaaaga attaggtgca gatgctatta
      841 ttaattctaa agatgttgat ccagttgcag aagttatgaa attaactgat aacaaaggat
      901 tagatgcaac agtggtaact tcagttgcta agacgccatt taaccaagcg gttgatgttg
      961 taaaagctgg tgcaagagtt gttgctgttg gtttacctgt tgataaaatg aacttagata
     1021 tcccaagatt agtgcttgac ggtattgaag tagtaggttc acttgttggt acaagacaag
     1081 acttacgtga agcgtttgaa tttgctgctg aaaataaagt aacacctaaa gttcaattaa
     1141 gaaaattaga agaaatcaat gatatttttg aagaaatgga aaatggtact ataactggta
     1201 gaatggttat taaattttaa aaatattaat tgattaaata tagatgaaga aggtagtgct
     1261 ctgaacacta tcattataat caaacacgag gttttcatga aagatagtga aaatctcgtg
     1321 ttttttggtt ttgaggtgtt gtttgtattt tataaaatgg cttacatata tgaagcgttg
     1381 attaagtatg gaattgttaa ttaattgaac ctatttagct ttaagaaggc ataacaagat
     1441 gaccttattt tatgctataa tatttctatt atgcgaagat taaggtgagt agtaaattgg
     1501 ataaaaaagt aagtattcaa acaaagcaag tgttgaaaca gcacaacgaa aaagaaaaat
     1561 ttgaatttac tactgaagga acttggcaac aaaggcaatc taactttatt cggtatgtag
     1621 aacaaattga ggatgcaaca gttaatgtta caataaaagt ggatgatgat agcgttaagt
     1681 tgattcgtaa aggcgacatt aatatgaatt tgcattttgt tgaaggacaa acgacaacaa
     1741 ctttttacga tatatcggct ggacgaattc cactagatgt taaaacatta cgcattttac
     1801 atttcgtaag tggagacggt ggcaagctaa agattcatta tgaattatat caagataatg
     1861 aaaaaatggg ttcttatcaa tatgaaatta actataagga gataggcgaa tgaatattat
     1921 tgatcaagtg aaacaaacat tagtagaaga aattgcagca agtattaaca aagcaggatt
     1981 agcagatgag attcctgata ttaaaattga agttcctaaa gatacaaaaa atggagatta
     2041 tgctactaat attgcgatgg tactgactaa gattgcaaag cgtaatcctc gtgaaattgc
     2101 tcaagcgatt gttgataact tagatactga aaaagcacat gtaaaacaaa ttgacattgc
     2161 tggtccagga ttcattaatt tttacttaga taatcagtat ttaacagcaa ttattcctga
     2221 agcaattgaa aaaggtgatc aatttggaca tgtaaatgaa tcaaaaggtc aaaatgtatt
     2281 gcttgagtat gtttcggcta accctacagg agatttacat attggtcatg ctagaaatgc
     2341 agcagttggt gatgctttag ctaatatttt aactgcagct ggctataatg taacacgtga
     2401 atattatatt aatgatgctg gtaatcaaat tactaactta gcgcgttcga ttgaaacacg
     2461 tttctttgaa gctttaggtg acaatagtta ttcaatgcca gaagatggct ataatggaaa
     2521 agatattatt gaaataggta aagatttagc agagaaacac cctgaaatta aagattattc
     2581 tgaagaagca cgtttgaaag aatttagaaa attaggcgta gaatacgaaa tggctaaatt
     2641 gaaaaatgat ttagcagagt tcaatacgca ttttgataat tggtttagtg aaacatcttt
     2701 atatgaaaaa ggcgaaattc ttgaagtttt agcaaaaatg aaagaattag gttatacgta
     2761 tgaagctgat ggcgctacat ggttacgtac aactgatttt aaagacgaca aagacagagt
     2821 cttaattaaa aatgacggta catatacgta tttcttacca gatattgcgt accacttcga
     2881 taaagttaaa cgtggtaatg acattttaat cgatttattt ggtgctgatc atcatggtta
     2941 tattaatcgt ttgaaagcat ctcttgaaac gtttggtgta gatagtaatc gtttagaaat
     3001 tcaaatcatg caaatggttc gtttaatgga aaatggtaaa gaagtgaaga tgagtaaacg
     3061 tactggtaat gcgattacat taagagaaat tatggacgaa gttggcgttg acgctgcacg
     3121 ttatttctta actatgcgta gtcctgatag tcactttgat tttgatatgg aattagcgaa
     3181 agagcaatct caagacaatc cagtttacta tgctcaatat gcacatgcgc gtatttgttc
     3241 aattttaaaa caagcgaaag agcaaggtat tgaagtgact gctgcgaatg attttacaac
     3301 aattactaat gaaaaagcga ttgaattgtt gaaaaaagta gctgactttg agcctacaat
     3361 tgaaagtgct gcagaacata gatcagcaca tagaattact aattatattc aagatttagc
     3421 ttctcatttc cataaattct acaatgctga aaaagtgtta acagatgata ttgaaaaaac
     3481 aaaagcacat gttgctatga ttgaagcggt cagaattaca ttgaaaaatg cattggcaat
     3541 ggtcggtgta agcgcacctg aatcaatgta agaacattta tatacactcc aacgtagagt
     3601 ttctcgaaag atactttgtg ttggagtgtt tttaattagg tatgtgacat attggggaat
     3661 gcttagtatg tgaataaggt taagaggaac gcagttggat gctactgcac aactgcataa
     3721 gagagcctga gacataaatc aatgttctat gctctacaaa gttataatgg cagtagttga
     3781 ctgaacgaaa attcgcttgt aacaagcttt tttcaattct agtcaacctt gccggcgggg
     3841 ccccaacaaa gagaaattgg attcccaatt tctacagaca atgcaagttg gggtgggacg
     3901 acgaaataaa ttttacgata atatcatttc tgtcccactc cctctaaaat ggagggtgta
     3961 aatgttagga actgatgaat tatataaagt tttatatgaa catctcggac cacaattttg
     4021 gtggcctgct gataatgaca ttgaaatgat gttaggtgca attttagttc aaaatactag
     4081 atggcgaaat gcagaaattg cattgaatca agttaaagaa cacacgcatt ttaatccaaa
     4141 tcatatatta gaactaccta ttgaaacgtt acaatcattg atacattcaa gtggctttta
     4201 taaaagtaaa tcactgacga ttaaaacatt attaacatgg ttagcacgac atcatttcaa
     4261 ttatcaagag attaatgagc gatataaagg tggattaaga aaagaattat tatctttgaa
     4321 aggtattgga agtgaaacag cagatgtctt acttgtttat atattcggac gtattgaatt
     4381 tattccagat agctatacaa gaaaaatata tgataaatta ggatatgaaa acactaaaaa
     4441 ttatgatcaa ttaaaaaaag tagtcacatt accaaatcat tttacaaatc aagatgctaa
     4501 tgaatttcat gctctgttag atgtatttgg taaacattac tttagagaca aagatataaa
     4561 gaattatgat tttttagaac cttacttt
//
LOCUS       DBNENW010000166         4542 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNENW010000166 DBNENW010000000
VERSION     DBNENW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.169"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            255..1034
                     /locus_tag="AC1LW8_002369"
                     /pseudo
     CDS             255..1034
                     /locus_tag="AC1LW8_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1183..2133
                     /locus_tag="AC1LW8_002370"
     CDS             1183..2133
                     /locus_tag="AC1LW8_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP4774160.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            2188..2907
                     /locus_tag="AC1LW8_002371"
     CDS             2188..2907
                     /locus_tag="AC1LW8_002371"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4774161.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            2891..3691
                     /locus_tag="AC1LW8_002372"
     CDS             2891..3691
                     /locus_tag="AC1LW8_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP4774162.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            3827..4333
                     /locus_tag="AC1LW8_002373"
     CDS             3827..4333
                     /locus_tag="AC1LW8_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SA0570 family protein"
                     /protein_id="HIP4774163.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
BASE COUNT         1679 a          587 c          836 g         1440 t
ORIGIN      
        1 aaaacagttg gatgctaccg cgcaactgca taaaagcctc taatcgctaa aggtgaagag
       61 gaacgcagtt ggcttgcttg cgcacaactg cataaaagcc tctaatcgct aaagctcaag
      121 aggctttaaa aaggagatgt gatagtgtga agaaatcgtt aattgctttt attttgattt
      181 ttatgcttgt cctgagtggc tgtggtatga aagataatga taaacaaggt agcaatgata
      241 atggctcgtc taaatcgccg taccatagaa ttgtttcgtt aatgcctagt aatactgaaa
      301 ttttatatga attaggatta ggtaaataca tagttggtgt ttcaacggtt gatgattatc
      361 caaaagatgt gaaagagggt aagaaacaat ttgatgcttt gaatctaaat aaagaggaac
      421 ttttaaagga aaagccagat ctaattcttg cgcatgagtc gcaaaaggca actgctaata
      481 aagtattgtc atcattagag aaacaaggca tcaaagtagt gtatgttaaa gatgcacaat
      541 caattgatga aacatacaac acatttaagc aaattgggaa attaacgcat catgataagc
      601 aggctgaaca acttgttgag gaaactaaag ataatatcga taaagtcata gattcaattc
      661 ctgctcatca taaaaaatca aaagtattta ttgaggtttc atcaaagcct gaaatatata
      721 cagcaggaaa gcatacattt ttcaatgata tgttagaaaa attagaagcc caaaatgttt
      781 atagtgacat taatggttgg aaccctgtaa cgaaggaaag tattattaaa aagaacccag
      841 atatattaat ttcgacggaa gctaagacaa gatcagatta tatggatatc atcaaaaaaa
      901 gaggtggatt caataaaatt aatgctgtca agaatacacg tattgaagtt gtaaatggtg
      961 atgaagtatc aagacccggt ccacgtattg atgaaggatt aaaagaatta agagatgcaa
     1021 tttatagaaa ataaaccatt ctaattatgc cccttattgc tacatgtaaa aaaatacatg
     1081 tttgagataa ggggttttta aaatatattt agtgaatgat agcaacgcga gtatgtgatt
     1141 gctataatga atgtaattat cgatgaaaga aaagagaatg ctatgacatt taataaagta
     1201 ttattgagct ggatagtcat attgattata acaactagca tatatctatt ttggcagttg
     1261 ggggatatca atgatgtatt taaccagtct attttaatca atgttagatt accgagatta
     1321 ttagaagcat tgttgacagg tatgatatta acagttgcag gtcttatatt tcaaacagtt
     1381 ttaaataatg cattggcaga tagctttaca ttaggattgg caagcggtgc tacatttggt
     1441 tcaggattag cattattttt aggtttaaca acgttatgga ttcctgtatt ttcaataaca
     1501 tttagtttga taacattaat aactgtatta gtcattacgt cggtattgag ccaaggctat
     1561 ccagttagaa tcttaatatt aagtggttta atgattggtg cattattcaa ttcacttcta
     1621 tattttttga ttttattaaa acctcgcaaa ttaaatacaa ttgccaatta tttgtttggt
     1681 ggctttggtg atgcagaata ctcaaatgta tccataatag caatcacatt tatcattgca
     1741 ctgtttggta tatttatcat tcttaatcaa ctaaagttat tgcaattagg agaactaaaa
     1801 agtcagtcac taggcttaaa tgttcaattg attacatata tcgcgttatg tatagcttct
     1861 atgataacgg cgataaatgt cgcatatgtt ggcatcattg gattcattgg tatggtaata
     1921 ccgcaactca ttagaaaatg gcagtggaaa caatcattag gaagacaatt ggctttgaat
     1981 attgtaattg gaggacaaat aatggttatg gcagatttta ttggtagcca tatattgtca
     2041 ccagtacaaa taccggcaag tattatcatt gcattaattg gtataccagt gttattttac
     2101 atgctaatat ctcagtcgaa acggttacac tagcacatga catttgctaa aataaaaata
     2161 actataaaca taaagagggc ataagcgatg gatttgaatc aaattaaagc agttgtattt
     2221 gatttagaag gtacgttgtt ggacagagtt aaatctcgag agaaatttat cgaagagcaa
     2281 tatgaacgat ttcatgacta cttaattcat gttcaactgg cagattttaa aaaagcattt
     2341 attgagctag atgacgatga agataatgat aaacctgatt tatataaaga aatcattaaa
     2401 cgtttccatg tagataggtt aacttggaaa gacttattta atgattttga aatgcatttt
     2461 tatcgttatg tatttcctta ttacgatact ttgtatacac tagaaaagct atcgcaaaaa
     2521 ggctttcaaa ttggtgttat cgcaaatggt aaatctaaga ttaaacaatt tcgattacat
     2581 tcacttggtt tgatgcatgt tattaattat ttatcaacat cagaaacagt tggttttcgt
     2641 aaaccacatc ctaaaatttt tgaagatatg attgatcaac taggggtatt acctgagcaa
     2701 attatgtatg ttggcgatga tgcgttaaat gatgtagctc cagcacgagc tatgggcatg
     2761 gttagtgtat ggtataaaca agaagatgct gaaattgaac cactcgaaga agaagttgat
     2821 tttacaatta caacagtgga agaattatta accattttac caataaaaaa tgataataaa
     2881 ggagaaaatt atggatctat ttactagaaa agatggaaca tcgattcatt acagtacatt
     2941 aggtgaaggc tatcctatcg tattgattca tactgtactt gataattatt ctgtgtttaa
     3001 taaattagca gcacaattag caaaatcatt tcaagttgtg ttaattgatt tacgtggaca
     3061 tggctattct gataaacctc gtcacattga aataaaagat ttttctgatg acattgttga
     3121 attacttaaa tatttataca ttgaagaagt tgcatttgta tgccatgaaa tgggtggaat
     3181 cattggtgcg gatatttcag tacgttatcc tgaatttaca tcatcactta tgttggtaaa
     3241 tccaacatct attgaaggtg aattaccgga agaacgttta tttagaaaat atgcccatat
     3301 tattcgaaac tgggatcctg aaaaacaaga taaattttta aataagcgta agtattatcg
     3361 tccgagaaaa atgaatcgat tccttaaaca tgtcgtagat acaaatgaaa tatcaactaa
     3421 agaagaaatt caagcagtta aagaggtatt caaaaacgct gatatttctc aaacttatag
     3481 aaatgtcgta gtaccgacaa aaattattgc aggagaattc ggtgaaagaa caacaagatt
     3541 ggaagctaaa gaagtagctg atttaatcca aaatgcggac tttgaagtat atcaagaatc
     3601 aagtgcattc ccatttgttg aagagcaaga aagattcgtc gaagatacag cttcatttat
     3661 caacaaacat cacgatgaaa agcatgttta atttttagtg ataagtgaaa tataaaagta
     3721 gtaattatta tttgtaatat aattgtaata tgactgttgt tttagaaatg attgttgtat
     3781 aatggttgtg agcacaagac gatataaata aaaggagaga gtgcttatga aaaagctact
     3841 aacggcaagt ataattgcat gttctgttgt aatgggagta ggcttagtga acactagtgc
     3901 cgaagcagca agtggcaact ctattgatac tgttaaacaa ttaattaagg gtgatcagtc
     3961 attagaaaat gtgaaaattg gcgaatctat taaagatgtt ttaactaagt acaaaaatcc
     4021 tatgtattct tacaatgaag atggaactga acattattac gaattccata ctaaaaaagg
     4081 tatgttatta gtaactactg atggtaagaa aaacaatggt aaagtaactc atatttcaat
     4141 gatgtataat gatgctaatg gtccaacata tcaagctgtt aaaaattatg ttggcaaagc
     4201 agtaacacat acggaatata gcaaagttgc tggtaatttt ggatatattg aaaaaggcaa
     4261 aacgacttat caatttgcct cagcaccaaa agataaaaac ataaaattat atcgtattga
     4321 tttggaaaaa taataattaa attaatcgtg cttaaccgac aagttaaatc gcaatacttg
     4381 tcggttattt tattacatgg cataatacag tgaatattaa tttttatgat ttgcgaaggg
     4441 gaaattaaac caatacaata aataaagatg tttcatttta aaggagtact accaatgcgt
     4501 attaacgata aaattttact agaaaatata gaagattact tt
//
LOCUS       DBNENW010000167         4535 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNENW010000167 DBNENW010000000
VERSION     DBNENW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.170"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..147
                     /locus_tag="AC1LW8_002374"
     CDS             <1..147
                     /locus_tag="AC1LW8_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIP4774164.1"
                     /translation="TPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSLVEAGYF
                     DEND"
     gene            215..499
                     /locus_tag="AC1LW8_002375"
     CDS             215..499
                     /locus_tag="AC1LW8_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774165.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(616..813)
                     /locus_tag="AC1LW8_002376"
     CDS             complement(616..813)
                     /locus_tag="AC1LW8_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774166.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            973..1446
                     /locus_tag="AC1LW8_002377"
     CDS             973..1446
                     /locus_tag="AC1LW8_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HIP4774167.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            1520..1639
                     /locus_tag="AC1LW8_002378"
     CDS             1520..1639
                     /locus_tag="AC1LW8_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774168.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            1838..2680
                     /gene="mreC"
                     /locus_tag="AC1LW8_002379"
     CDS             1838..2680
                     /gene="mreC"
                     /locus_tag="AC1LW8_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIP4774169.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            2680..3210
                     /gene="mreD"
                     /locus_tag="AC1LW8_002380"
     CDS             2680..3210
                     /gene="mreD"
                     /locus_tag="AC1LW8_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIP4774170.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     gene            3572..3880
                     /gene="rplU"
                     /locus_tag="AC1LW8_002381"
     CDS             3572..3880
                     /gene="rplU"
                     /locus_tag="AC1LW8_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIP4774171.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            3886..4206
                     /locus_tag="AC1LW8_002382"
     CDS             3886..4206
                     /locus_tag="AC1LW8_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIP4774172.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            4218..4502
                     /gene="rpmA"
                     /locus_tag="AC1LW8_002383"
     CDS             4218..4502
                     /gene="rpmA"
                     /locus_tag="AC1LW8_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIP4774173.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         1635 a          608 c          845 g         1447 t
ORIGIN      
        1 acgccctcac aagaagatat cataacaaca atgaggttga aggagtgtgg tttgatttta
       61 gggatagatt tattggatca tattataatc ggtgataata gatttaccag tcttgtagaa
      121 gcgggttact ttgatgaaaa tgattgaagt tgatagaatt aatgacgtat cttgtgtata
      181 atacctacga agtactttca ttggaggaaa aatagtgact ctatttatta ttatcggggt
      241 tctcgtgcca atggtttata ccatgcagtt aaatattaaa aatgaacctg taacaaagcg
      301 caatctatta ataacattag ctttatctac gttaggtatt ttagtaacgg cgttagcagg
      361 tgtgatcgtt acgaaacaag cttttccttt attaagtgta gcaatcggct caatttttac
      421 tggaatcgtt tggggccttt tactaagtgg tagctacgcg ctgatacgat ttttatctaa
      481 cgcatttggg cgtaagtaat agtaatctgt tcatcaagta gtatccgtgc ttgaaaccaa
      541 attaaaactc ctaatgtgga actagttaaa aaattctaaa ctagtacatt aggagttttg
      601 ttatgcagaa taaatttaat tgttaaattg aaagtgcggt aaaaacccac tatttatttg
      661 aaaaaaatcg agcaaccaaa ttaaaccttg atatcctaag taaatacata ataaaacaag
      721 tccgacataa attaaaaatc gcaaaataga aagtccaact ctaaaaagga ggatgactaa
      781 tagtgctatt aaaataattg ttagtatact caatgctaca aacctcctca tacgctttta
      841 aatcataaca aaattataag ctattttaaa tgaacgtgca tcagtcctaa gatatatcat
      901 gattaaggct gaagactgag gaaaatatac ataataaaat atataattag atgaagacat
      961 tggaggcgaa ctatgagatt gatatttagt atgattaaaa acattatagc agtaatagca
     1021 attgtgataa ttgtttatat cgccttgaaa tacgcacctt ttttaaaaga acaagattgg
     1081 aatcctgtaa atcacgataa tgatcaaatg aatcaagtta cgcaacccac taatgatgcc
     1141 caacatgttt atgtaagtgg tgagaaatat tcattaaaag aaaatgattt aataaagaat
     1201 gtaccattaa gccaaattaa aaatgtattt aaaatgatag ataaacaaga gtttatggct
     1261 gtgtctggaa tgaatcgcat ggcttataat gatcaatata ttataggtca aagaggagac
     1321 gaatttattc tttataaatt tggagatgag tcaatgcgtg tttacaatac tgaatttgaa
     1381 atgcaacaag acttaaatga attagggcaa aatttacaac taaaacccga aaatgcttat
     1441 caataggaaa ttattgcata agaaatatat tttagttatc ctgtcactag aataaattta
     1501 aaaggttggt gtagttcaaa tggcagaaca atcaaaacag aaacaagcta atgaacaaca
     1561 aaaggcccag aacttattcg cacgttggag acaacttcag aattcgaaca gtgaatcctc
     1621 gaacgataca aacaaataat tttaaaattt aaatcgatta tttaaactac tagtgactgg
     1681 aatgtttgct agtagttttt tgtttttaaa atgccacaaa ttttttacgg agttgttcat
     1741 tttcaaagta attcgtagtt agtgctttat ttcataatgg gagtaatata aaatagaaaa
     1801 ggatatacaa tattttgaca taatagaggt gttctgggtg cttaagtttt ttaaaaataa
     1861 caaattaatt gttgttttat gtgcaattat cgtttttatt gcattaattg ggctgtccat
     1921 acgttcacaa tctcaatcac ctcctgaaca atatataggt gattctgtgt cttttggaca
     1981 acgagttgtg agttatccag ttaattttgt tgctggtacg attggggact tttttaaaaa
     2041 aggagattct aaagaatcta aaaataagat tagccagtta gaatctaaga accaacaatt
     2101 agaagcggaa aatgaaaaat taaaaaaaga gcttgattta aaagatattt caaaatttga
     2161 tcctatttct actacggttt tagcaagaaa tccggatcag tggatgaata caattgtaat
     2221 tgataaggga tctaaatcag gtataacttc aaatatggct gtgatgacat cacaaggttt
     2281 tgttggaaga gttactaaag ttaataaatt ttcttcacaa gttgatttaa tctcaactaa
     2341 tacacgtgcg ggtaaattat ctgtaaatat acaacacggt tctaaaaata tatttggttt
     2401 aattgatcgt tatgatgaaa agaactcaga acttgtaatt agtgacatta ataatagaga
     2461 taatatctca aaaggtgata aagtcgttac aagtggatta gctgatcaac taccaagtaa
     2521 tttatatata ggagaagtga ctaaggttca aaatgatcaa tacggcttag ctaaagaagt
     2581 tagggttaag actggtgcag acttaacaga tttgagtcat gtttatgttg caaaaagaga
     2641 tcctaaaaca attcctgatg atgaaagcag ggataaataa tgcgtacact gtattatttt
     2701 ttgataggta ttttactatt ttatatagat actgcaattg ggcttcttat tccaatgcat
     2761 ataggtaagt ttgaacttgt atttgtacca caccttacat ttatgtatat tttaatgatg
     2821 gtagtgtatc gtggctttgg cgtatcatta ttgctcagca tatttttagg cgtaatgact
     2881 gatgtatact ttggtagtat ttatggagtg tacttatttg gctatatatt gtttttagca
     2941 cttatagatc gattctttaa aattttttac aaagatcatt cgatgttatt cattattata
     3001 ttagccagca ccttattatt agaagtctat gtggcattaa tatacggtat gttaggattc
     3061 attcaatttg atattattca ttttgtagtc tttagattat tgccaacatt aattatgaat
     3121 tttgtactgt tgattatgct ttatccgttg attataaagt tccttaaaaa aacaaacaat
     3181 gacattgaca tgaaacgtcg tcaatggtaa gatgcaatag ttgagtagtt ataagtagct
     3241 acatacaacc gctcaaatat aggttcaaga acatttttaa tgaagaaagc gattgtcaat
     3301 caaggcgttg aaggaagaaa tgagaagagt ttatgtttat aaatgactcg agttgcgacc
     3361 gaaacaacgt agagtgcaaa cgcttgactc atttgcagaa gttattgaaa gaaagcgatt
     3421 gtcaatcaag gcgttgaagg aagaaacgag aagagtttat gtttataaat gactcgagtt
     3481 gcgaccgaaa caacgtagag tgcaaacgct tgactcaata acgaaatgtc acctatgtat
     3541 ggcgtgactt attatacagg aggtgcaaag tatgtttgct attattgaaa caggtggaaa
     3601 acaaatcaaa gtagaagaag gtcaagaaat cttcgttgaa aaattagacg taaacgaagg
     3661 agatactttt acatttgata aagtattatt tgtaggtgga gattcagtta aagttggagc
     3721 gccaacagtt gaaggtgcaa cagttactgc tactgttaat aaacaaggtc gcggtaaaaa
     3781 aatcactgta ttcacataca aacgtcgtaa aaattcaaaa cgtaaaaaag gccatcgtca
     3841 accatacact aaattaacaa tcgataaaat caacgcgtaa ttattatgat tactgttgat
     3901 attacagtta atgatgaagg caaagtaaca gacgttatta tggatggcca tgctgaccat
     3961 ggtgaatatg gtcatgatat cgtttgtgct ggagcttcag ctgtattgtt tggtagtgtt
     4021 aatgcgatta taggattgac atctgagaga ccagatatca attatgacga caatggtggt
     4081 cattttcata taagaagcgt tgatacaaat aacgatgaag cgcaactaat tcttcaaaca
     4141 atgcttgtgt ctttacaaac tattgaagaa gaatataatg agaatattag attaaattat
     4201 aagtgaggtg cattccgatg ttaaaattaa acttacaatt cttcgcatct aaaaaagggg
     4261 taagttctac aaaaaacgga cgtgactctg aatcaaaacg cttaggtgct aaacgtgctg
     4321 acggtcaatt cgtaacaggt ggttcaattt tatatcgcca acgtggtact aaaatttacc
     4381 ctggtgaaaa tgtaggtcgt ggtggcgatg atacattatt cgctaaaatc gacggcgttg
     4441 ttaaattcga acgtaaaggt cgcgacaaaa aacaagtttc tgtatatgca gtagctgaat
     4501 aattttgtct agttaacacc agaagtgaat cttct
//
LOCUS       DBNENW010000168         4442 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNENW010000168 DBNENW010000000
VERSION     DBNENW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.171"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(31..579)
                     /gene="hxlB"
                     /locus_tag="AC1LW8_002384"
     CDS             complement(31..579)
                     /gene="hxlB"
                     /locus_tag="AC1LW8_002384"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - 6-phospho-3-hexuloisomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HIP4774174.1"
                     /translation="MAEFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(581..1213)
                     /gene="hxlA"
                     /locus_tag="AC1LW8_002385"
     CDS             complement(581..1213)
                     /gene="hxlA"
                     /locus_tag="AC1LW8_002385"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HIP4774175.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(1290..2048)
                     /gene="nagB"
                     /locus_tag="AC1LW8_002386"
     CDS             complement(1290..2048)
                     /gene="nagB"
                     /locus_tag="AC1LW8_002386"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIP4774176.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            2324..2686
                     /locus_tag="AC1LW8_002387"
     CDS             2324..2686
                     /locus_tag="AC1LW8_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HIP4774177.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            2700..3365
                     /gene="bshB2"
                     /locus_tag="AC1LW8_002388"
     CDS             2700..3365
                     /gene="bshB2"
                     /locus_tag="AC1LW8_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HIP4774178.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            3378..4256
                     /gene="folE2"
                     /locus_tag="AC1LW8_002389"
     CDS             3378..4256
                     /gene="folE2"
                     /locus_tag="AC1LW8_002389"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIP4774179.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT         1442 a          807 c          714 g         1479 t
ORIGIN      
        1 ctaggcatca tattatcgac tatctttatt ttattctaaa ttagcatgat tttgttgcat
       61 cgtttgttcc gtaacattca tttcagtcat caatcccatt acaacactat ctaaaaataa
      121 ttgagatgct tgttcaaaca aacttcctaa tggttgtgcc gagccttgtt catcatattt
      181 tgtacctgca ggcaaaacga tgttcgtccc agctagattg cctattgcag aatctttatt
      241 tgtagtaatt aatacgatgt cagcacctac tgattttgct ttgtctgcta ataatcttaa
      301 atgttccgtg gaacctgaac cagagataat tacaaataca tcattcgact taatcgcagg
      361 tgtcgttgat tctccaacaa catgtgcctg tttgccgagc tgatttaagc gcattgcaaa
      421 actattcgcc acgaatcctg aacgtccttt accagctaca aatatatgtt cagcatgtag
      481 tattttggat gcaaacgttg aaaactcatc cgcttcaaca tgtgacaaag tcatctttaa
      541 ttcatctaga attaattgat agtcactaaa ttcagccata ttacttacct tcgattgcag
      601 cgcgacattg ttttgcagct tctactggat catctgcatt tgcgattccg ccaccaacaa
      661 taacaagatc aggactttca gcgacaattt ctttaattgt atctggttta attccacctg
      721 ctactgcaac tttagaattt ttaataacag atttaacggt tcttaaactt tctaatggtg
      781 attgcccttc tgcttgtaaa tcataaccag tgtgtactgc aatataatcc gcacccattt
      841 catctagttc ttttgcacgt ttttctaaat cttgaacagc aatcatatca actagtaatt
      901 gtttattatt tttatgagct tcttcaatag ctgctttaat tgatgcatct tctgcaacac
      961 ctagtattgt aattacatcc gcgccaaatt taattgcttg gctaacttca taatcagctg
     1021 catccataat tttcatgtct gctaatactt ttacattact aatgttgtct gccatatgtt
     1081 taactgctgg taaaccttcg ttgtaaatga ttggcgtacc gatttctacg atatctacat
     1141 aatcttttac tttatttgct aactcagccg cgtcttcttt gtttaataaa tcaattgcta
     1201 attgtaattc cactaaaaat catccttcca atgcttttta tctttctatt ggaacctttc
     1261 ccgaggcatt tcttaattaa acattgcaat caatccattt catcgacatg aattttcgca
     1321 acatttttcg ggcaagcttc gtcgtctaaa tatatctcaa cattcgggtg tttgtgaagt
     1381 aatgtggctg gaacatcaac agaaatttcc tgatttaata aatgtgtaat agcagcacgt
     1441 ttcttttcac caaatgcgag taaaatgata cgtttggctt gaagaatatt agcaagtccc
     1501 atcgaaatgg cttgctttgg aacatcatct tcgtttttaa aatatcgact attagcctta
     1561 atagtacttt cagtcaaatc aacgatatga gtaacgctgt caaacggcgt accaggttca
     1621 ttaaatccaa tatgaccatt ttctccaata cctaaaattt gaatatcacg ttgaccttgt
     1681 tgttctaaaa cgtcattata ttttgacgct tccgcattca tatcatcggc atctccattt
     1741 ggaatatgaa tgttctttct attaaaataa ggatattgtt tgaaaagcat gtcatccata
     1801 taatagtgat aactttgcgg atgtgatgcg gttaaaccta catattcgtc taaattaaac
     1861 gtggatacat tgtctacgtt taactgattt ttatttaata acttaacaag ttgctcatac
     1921 aaatctgtca ttgtaccacc agttgctaaa ccaagtttac agtgctgatt aaatgccatc
     1981 tctttatata actcacatgc aacatagaat gatgcttgtt ttttcgatcc taagtttaat
     2041 actttcattg tttacatgcc tccacaaatt taatacattt taaaaactta tgaattcatt
     2101 catacaccat taatatagca tcatttttaa tgtttaacac ttttcaaatt ccaacaaatt
     2161 tttgattttt aaaagtaaaa aactaatgct gtatgattgc gtaatcctga gtaattaccc
     2221 ttcatgaaaa ttaatttata aataattgag caatcgtata caattattga atagttttta
     2281 gtaaaataga aagataaaca cattgatagt gtaaggagtg aacgtgttgg aaccaattaa
     2341 agaacaagaa gttatagatt tacttgcttc ttttgaacat aaacctgtgt atctgcatgt
     2401 tgaaacgaca aatggggcct atgcgaacca tttcgatcaa cgtgttttta atgctggtac
     2461 atttttaaga aacatacaag taacatatac gcatgcacaa ctaaaaggtg gtaataaaga
     2521 accttatcgc attggtctta aactatcaaa tggtggttgg gtttatgtac aaggtctaac
     2581 gcattttgag gtcaatgaac atgatgaatt tttaattgca ggttttaatt acgaaggtca
     2641 actcgctgca gcacttcaaa ttagtgagcg accatttaat ttatagagga ggcatcaaca
     2701 tgactgacga aagacatgta ttagtaatat ttccacatcc tgatgatgaa accttctcat
     2761 ctgcaggcac attagcaagt tatatccaaa aaggcatacc tgtaacatat gcatgtttaa
     2821 ccctaggaca aatgggcaga aatttaggta atcccccatt tgcaacacgc gaatcattac
     2881 caagtattcg tgagcgtgaa ctagaagaag cttgtaaagt tattggtatt actgatttaa
     2941 gaaaaatggg tttacgtgac aaaactgtcg aatttgaacc ctacgaacat atagatggta
     3001 tgattaaatc tttaatcgat gatacaaatc catcattgat tatttcattt tatcctggat
     3061 atgcagtaca tcctgatcac gaagcaacag cagacgctgt aattcgaacg gttgaacgca
     3121 tgcctaaaga agaacgccct cgtttaactt tagttgcgtt cagtaatgat gcaaccgaag
     3181 ctcttggaga acctgatatt caaaatgata ttacagattt taaagaactt aaaattaagg
     3241 catttgaagc acatgcttct caaacaggtc catttttaaa acaattagca agtcctgaaa
     3301 tcgatggtaa acaacacagc tttttaacag tcgaaccata ttggacatat cactttaaat
     3361 cttaacggag gaaaaaaatg actgaatttg atttatcgac acgagaaggt cgttggaaac
     3421 actttggttc cgtagatcct attgaaggca cgaaacctac tactaagaac gaaatgacag
     3481 acttacaaag tacacataaa gattttttat ttgaaataga agaagttggt attaaaaact
     3541 tagtgtaccc tgtacttgtt gaccagtatc aaacagctgg cacatttagt ttttcaacaa
     3601 gtttaacaaa agacgaaaaa ggtattaata tgagccgtat aattgaaagt gttgaaaagc
     3661 actatgataa tggtattgaa cttgaattta atacacttta tcaggtgtta cgcactttac
     3721 aaacgaatat gaaacaaaat gcggcaggcg ttgatgtttc tgggaaatgg ttctttgatc
     3781 gctatagtcc aaccacaaat attaaagcag ttggtaatgc cgatgtcact tatggattag
     3841 caattgacgg tgataaagtt acacgtaaag aacttactat tgaagcaact gtcacaacgc
     3901 tatgcccttg ttcaaaagaa ataagtgagt attcagcgca caaccaacgt ggtgttgtca
     3961 ctgttaaaac atatattaat aaagatcaag acatagttga tgactataaa aataaaattt
     4021 tagatgcaat ggaagctaat gcaagttcaa ttctataccc tattttaaaa cgtcctgatg
     4081 aaaaacgtgt gacagagcgt gcatatgaaa atccacgttt tgtcgaagac ttaattcgtt
     4141 taattgccgc ggacttagtt gaatttgatt ggttagacgg tttcgatatc gaatgtcgta
     4201 atgaagagtc tatccatcaa catgatgctt tcgctaaatt aaagtatcgc aaataatatc
     4261 atatgattaa ttcaaacgcg taacagtagt tgcgcgtttt tattatacct tctagagttg
     4321 tatatctctt ttataaatct tctcatttga tgtgcctgag tcataaatca atttcctaaa
     4381 ctctacaatg atatattggt cgtagttgac tgaataacta ctaccattat aaatatcaaa
     4441 ac
//
LOCUS       DBNENW010000169         4423 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNENW010000169 DBNENW010000000
VERSION     DBNENW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.172"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(261..866)
                     /locus_tag="AC1LW8_002390"
     CDS             complement(261..866)
                     /locus_tag="AC1LW8_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIP4774180.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGLVASRRAQTLSQYVEHIMVISPEI
                     TEKLQNMVDKGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDMNHSEKQQLLSQIVTKEFINDTKQAQFLAWLDKK"
     gene            complement(929..2809)
                     /locus_tag="AC1LW8_002391"
     CDS             complement(929..2809)
                     /locus_tag="AC1LW8_002391"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HIP4774181.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLLLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQQQRYQRD
                     VY"
     gene            3004..3096
                     /locus_tag="AC1LW8_002392"
     CDS             3004..3096
                     /locus_tag="AC1LW8_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774182.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(3204..3701)
                     /locus_tag="AC1LW8_002393"
     CDS             complement(3204..3701)
                     /locus_tag="AC1LW8_002393"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIP4774183.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(3711..3971)
                     /locus_tag="AC1LW8_002394"
     CDS             complement(3711..3971)
                     /locus_tag="AC1LW8_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774184.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
BASE COUNT         1334 a          841 c          608 g         1640 t
ORIGIN      
        1 tttaatagca atggttgaat tctcggttct attacacaaa aaaactggct aaaattagct
       61 attattcata gcaacttcaa ccagtttttc taataaccta tatatttatt aagatttttc
      121 aaatcgtcta tttctctatg ttagaaatca tcttacatat gtttatcatc ttcgcttaga
      181 aattattagt atgcatccca tcttcattta caaacagaca tgttattact tcaagactgc
      241 ctttagacgg cacacagtta ttatttttta tccaaccacg ctaaaaattg agcttgttta
      301 gtatcgttta taaattcttt cgtcactatt tgtgataaca attgctgctt ttcgctgtga
      361 ttcatatcaa gtaactttat tttctgtcgg caagtatata aaaagtcgat atacgaactg
      421 tatgatggtg gatataacgc ctcaagctct gccataattg attttgtcaa cttaggactc
      481 gcaccatcag ttgatacact gatagttagc ttgtcgcggt gtagtgcact tggaaataca
      541 acattgccat ttgatgcatc tccaacatta ttaaaaaggg catgctcagg taaggcttgt
      601 tttaccgctt cattgacacg tggctcattg gttgctgcaa taactagata cgcgtctaca
      661 atatcgcttg gttcaaactc tttttctttc catatgacga cacctttatc taccatattt
      721 tgaagttttt cagtgatttc aggactgata accattatgt gttcaacgta ttgacttaat
      781 gtttgtgccc gacgacttgc aacgaggcct ccaccaacta tgacgacatt tttatttgtt
      841 aaatctatca ttaatggcat attcatgttg agcaacatcc tttatatctt tttacgtcat
      901 ttcatcttaa aacatttaac accaatcgct aataaacatc acgttgatag cgttgttgct
      961 gtttcatttg acgcaatatc gtctccgctt cttcttgaga aatatgacgt tcttttacca
     1021 atacatcttt aatggcttga tggacatctt tcgccataca tttttcatca ccacaaatat
     1081 agattgctgc gccttgctca atccattcat tgaaacgttc actttcttcc attatacgat
     1141 gctgtacata ttctttgtgt tcttggtctc ttgaaaatgc taaatctacg cgtgttaagt
     1201 ttccattttc aagccattct tctatttctt cttcatataa aaagtcagaa ctacggtgtt
     1261 gatcgccaaa gaacaaccat gttttcccag tcataccaag ttcttcacgt tcttgtaaat
     1321 atgctctaaa aggagcaatt ccagtacctg gtccaatcat aataaccggt atatcttgct
     1381 tcatcggaaa tttgaagttc ggatttttct tcaaataaat tggtactata tcgcctggtt
     1441 taattcgctc agcaaaatga accgagcata caccttttct ctcacgtcca tgtgcttgat
     1501 aacgaaccgt accaacggta atatgcactt catctggcgt tgccataaaa ctactagaaa
     1561 tcgaatactc tcttggtggt aattttctaa ggatttgata catattctca ggttgtaatt
     1621 ctatagttgg aaaatctgtt attaaatcta taaagtcccg atttataaca tattcacgcg
     1681 cccatgactc atcttgaata cgttcagata attcttcatt gtcaaaatag atatctgcat
     1741 ttttcaataa tagcaatgtt aatttagtaa attcaaaatg tgatgttagt gcttcaacaa
     1801 taggaactgt atcaccatga tcattaattg gcaccggaga ttgcggatcc caacctaaca
     1861 tggatattag tttttcaacc aattcagggt cgttttgcgg taatgctact atacaatctc
     1921 ctggttcata tgattcacta aaatcatcaa gtaaaaattc tatatgtcgt gtttctttat
     1981 ttgaatcggt accatttaaa ttgatattcg ctaaaacttc tgcttggtat ggatttaatt
     2041 tagaatattt cttttctttg gcagacttaa ttgattcact tatcaccgat tcactttgaa
     2101 taccttctga tgtagtatca atcatattaa tgatgtctgc catccacttt tctgcgtctt
     2161 cttcataatc aatatcacaa tctacacgct tacatatacg ctcagcgcct agattttcta
     2221 gtaaagcatc tacatcttta ccagcttgac agaaaaattc atatgtttga tcacctaaag
     2281 ctagtactga atatctcaca tgatttaaat taggtgcgtt atcgtcttct aaaaattcaa
     2341 agaaatccca tgcattatca ggcggttctc cttcaccatg agtagacgta ataataaata
     2401 aatcttctaa ctgcgcgatg tttgtcgtat catattcatc cattgacatc aaaacaactt
     2461 gatgtccgat atcacttaaa cgttctgaaa aaatttcagc taaacgcatg gcattacctg
     2521 attcagaacc atatagcact gtaacatgcc gctgattagc ttcgatatgt ggctctttat
     2581 tttgtaacat atacgttgtt gaagttgctt gttcttctga tatttgtgat tgttgccaca
     2641 cttctgtctc taattgttgt tgattactat cacttgtcgt atcgttacta ttagctagta
     2701 aaaaaccact aagccactgt tgttggctct ctgttaatgt ttgcagcaga ttgttaatct
     2761 ctgtgacttg tttttctgta aatggactat tagatgtatt taatttcaaa tgttttcacc
     2821 tcaataaact tgaaatagat gattgactct ttttttatcg atagtttagt caatttttta
     2881 atatgtatgg tataaattaa aacaaacttg agttgtgtct gatagttata cttgcaaata
     2941 tttacaactt tcatgttgct tgaatgacat tatcagtaat tcagacaaaa ctttgattat
     3001 gtcgtgtcag ttattaatgc atacagtaat gcatcgttgt tattttttat ttatttacta
     3061 tttattatgt accaaatcag cctttcagta aaataggtca aattactgat tttctaaata
     3121 taaaatgcct cctaatgaca tactattagt acatcattaa gaggctcttg tgttatttgc
     3181 atactaagcg ctcaatttta aatttaagat gaagattctt gcaataatat ttctatattt
     3241 gtcgatatat ccaatggatc ttccattggc aagaatcgat ttacaacatt gccttgtcga
     3301 tcgattataa attttgtgaa attccatttt attggggacc caaagattcc tggttgttca
     3361 ttctttaaat gcgtaaataa cggatgttca tcgttcccgt tcacagatat tttagctagc
     3421 actggaaatg taacaccaaa tttctcacga ctaattttca agatttcttc attagaacct
     3481 ggttgtcgat tgtcaaaatt attattcgga aaactcaaca ctacaaaccc acgatcctta
     3541 tatttttgaa acaaagtctc tagttttttc aattgttcgc tatatataca ttctgttgca
     3601 gtattaacaa ctaaaatcac tttacctcta aatgcttcta atttataagt taagccttta
     3661 taatcactta cttcgatatc atacacattt ctattattca taagacaccc ctacacagcc
     3721 ttttttatat tgaatatgtt ctttttagaa tgttctgata aaataagtgc gcgtttacac
     3781 cgcgaacata cattatatag cgtgatacat ttttcgagca cacgataaat aatgttcgag
     3841 tttatgttgt tgctcaacct atccgattta ccgtcttttt tcacctgtct tactttatta
     3901 aaataatcac attgtatagc tgtattagtc cagctacaat atctttcgtt ataacccctc
     3961 attataatca tccttatttt ctatttttaa aaagacaatt agaccgctct ttaaactata
     4021 gattaatact taagttaaac tcatacatac tgataccata cgttagatct aacaatttaa
     4081 aattcgttat aactatggat taaagagctg cccaactcat ataatcctta aaaacttcac
     4141 atgtgattgt ttattaagcc ctcctttatc ttattaaata tccttataac ccttttaaaa
     4201 ttaaactgac acactcatac attgttacac gcgacgataa acgtcatatt attgcatatc
     4261 attaaaacaa gtcactttgt atcgtgtgcg accgaataca tattaaatca tcaataatga
     4321 ttattcaatt attaaaaata aaaacgaaca ttctatatag agtttctact tattgttttt
     4381 tcatcattgt ctacattact taacattcgt gtttataagt aat
//
LOCUS       DBNENW010000170         4418 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNENW010000170 DBNENW010000000
VERSION     DBNENW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.173"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            127..1164
                     /locus_tag="AC1LW8_002395"
     CDS             127..1164
                     /locus_tag="AC1LW8_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP4774185.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGFIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKTEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLQAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1221..2045
                     /gene="sph"
                     /locus_tag="AC1LW8_002396"
                     /pseudo
     CDS             1221..2045
                     /gene="sph"
                     /locus_tag="AC1LW8_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004767 - sphingomyelin phosphodiesterase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2283..3299)
                     /gene="lukG"
                     /locus_tag="AC1LW8_002397"
     CDS             complement(2283..3299)
                     /gene="lukG"
                     /locus_tag="AC1LW8_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HIP4774186.1"
                     /translation="MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3321..4373)
                     /gene="lukH"
                     /locus_tag="AC1LW8_002398"
     CDS             complement(3321..4373)
                     /gene="lukH"
                     /locus_tag="AC1LW8_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HIP4774187.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKEKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKT
                     VKVVDKYSDNKSFREG"
BASE COUNT         1498 a          715 c          650 g         1555 t
ORIGIN      
        1 attccatttg gatgtcgata tctcttataa agaaacaaaa taaacctatt aattatatta
       61 taatcattat ttttcgggta gctcgcctac ccttattatt ttttgccaat tttgaggagg
      121 gaacgcatga aaacacgttg ttacgatggt aaaaaatggc aatatgaatt taagcatgaa
      181 ggaaaaagat accgtaagaa aggttttaga acaaagcgtg aagctaattc tgctggacta
      241 gacaagttaa atgagttaag aagtggtttt aatatagata actatataac tcttgaagaa
      301 tacttcgaaa attggattaa aacgtataaa caacctgttg ttaaagaaaa tacctaccgt
      361 cattatagaa atgcattaca acatatacaa aaacataaaa taggtaaaat ggagttatca
      421 aagataaata gacaagttta tcagaaattc ataaacgact attcaaaaga acacgcaaaa
      481 gaaactataa gaaaaacaaa cggtgctatt cggtcagctt tagatgacgc attatatgat
      541 ggatttattt ttaaaaaccc cgcttataaa gttaattata aagccggaaa acctacgaag
      601 tcagaacaag aaaaattcat ctcggtaact gaatatgaaa tactaaaaga tcacgtcaga
      661 aagaagagaa ctcgttcatc attagcgtta ttcataatga tttgtacggg ttgtcgtgtc
      721 agtggtgcaa gaaatataaa gactgagcat atcaaccaag tgaaaaacac tatatttatt
      781 gacgagcgaa aaaccgatac ttcccctaga tatatcagta tcgctaaatc tgatatgaaa
      841 cacattatgg acgtcataag tacatttgca attagctatg atggttacat tttcaaagaa
      901 gccggatcta taattaacct tcaggctatc aataatgctt tgaaatcagc ctgtagagtc
      961 aataatatac caattattac atcgcacgca ttaagacaca ctcattgttc ttatttacta
     1021 gcaaaaggtg tatctataca ttacatttct aaaagattag gtcataaaaa tatagcaata
     1081 actacatccg tgtattctca tttgttagaa gaaaaattta atgaagagga caaaaaaaca
     1141 actaaaattt tagaaagtat gtaatttagg gacccattag ggactccaaa cccaataaat
     1201 actgttgtta caaggtttct atgtatccaa actgggggca atataaacgc gctgatttaa
     1261 tcggacaatc ttcttatatt aaaaataatg atgtcgtaat attcaatgaa gcatttgata
     1321 atggtgcatc agacaaatta ttaagtaatg tgaaaaaaga atatccttat caaacacctg
     1381 tactcggccg ttctcaatca gggtgggaca aaactgaagg tagctactca tcaactgttg
     1441 cagaagatgg tggcgtagcg attgtaagta aatatcctat taaagaaaaa atccagcatg
     1501 ttttcaaaag cggttgtgga ttcgataatg atagcaacaa aggctttgtt tatacaaaaa
     1561 tagagaaaaa tggtaagaac gttcacgtta tcggtacaca tacacaatct gaagattcac
     1621 gttgtggtgc tggacatgat cgaaaaatta gagctgaaca aatgaaagaa atcagtgact
     1681 ttgttaaaaa gaaaaatatc cctaaagatg aaacggtata tataggtggt gaccttaatg
     1741 ttaataaagg cactccagag ttcaaagata tgcttaaaaa cttgaatgta aatgatgttc
     1801 tatatgcagg tcataatagc acatgggacc ctcaatcaaa ttcaattgcg aaatataatt
     1861 accctaatgg taaaccagaa catttagact atatatttac agataaagat cataaacaac
     1921 caaaacaatt agtcaatgaa gttgtgactg aaaaacctaa gccatgggat gtatatgcgt
     1981 tcccatatta ctacgtttac aatgattttt cagatcatta tccaatcaaa gcctatagta
     2041 aatagtgctc aactaactaa taactcgctt cgttctaaaa ggtcgaagcg agttatattg
     2101 ttaaaatttg aattgactta cattttaata aaatcatctt aacaatttta atttttcatt
     2161 aatacaagtc tttactctac actcaaacaa gattcataca ctgcacatca taataaatct
     2221 atctattcaa atataaataa aagttaccta ctacattcta tgtagcaggc aacttttatt
     2281 acttatttct tttcattatc attaagtact tttacaaact tcacattatg tgtcttccaa
     2341 tcaacttcat ataatgctga taatttttct tcttttttat ctacatggtt ttcaccagac
     2401 caatagcccc agaaaccatg acgattccaa tctattttaa actcatccat tgatctttta
     2461 taatgaacaa caaattttga tttaccttca tcttttttat catgtgacat aacagctaaa
     2521 aattctggat taaacccttc agaaacagtt acaggcatct tattcttagg agtaaaatta
     2581 tctttcgccc ataaatttcc atttcgtgtt aaagaaaaga tttcactttt agttctatta
     2641 tcactatcat tagttaattg tctcgtatgg tcatgtccca tattatttat caaatgtgct
     2701 tctactttcc aacctacacc tttatgtgac gtagattgat caagtaatgt acgatacgat
     2761 ggttgttgat aactaatcgt ctctgaataa ttactctctt ttgtaatatt tccagttaac
     2821 cctccacgat taatcgaaaa atctccacct gttttataac catacgtata tttaacttct
     2881 cttgattcat cctgattttt tggtgcaaag tcagtcacat ttgtattgtt gttgtcatca
     2941 acattttgaa ttgaaactga ataagatcca ggccatctta atgtactatt ccaataacca
     3001 tttgggtcta gaattcttaa accactacct attgttcctt tcgctttaat aaatactgtt
     3061 tctttatcat aattaggttc agttaagaag ttaaattgta agctttgagt aatatttttt
     3121 tgactatcac ttgttgtagc tgtacgtgta tacattttag tatcaccatc aagattcttc
     3181 tcagaaacag ctttaatttc agaattaatt tttgcataag aagtagctgg taatacagtg
     3241 aaagtagtcg atagtgctaa cgtacaaatt gtgatatttt tatatagttg tttaatcatt
     3301 tgtaatcctt cccttcgatt ttatccttct ctaaatgatt tattatcaga gtatttatca
     3361 acgactttaa ctgttttatt tttccaatca acttcataag tgacaattaa tctttgacct
     3421 tctttatttt tttctaaaat tggaggtgca taatgtattc caggtctgtt tttcaaaata
     3481 tcttgatttc gtgtgtatgt tacttcaaat tgcgttttct catttgactt ttcattagat
     3541 aaataagtta aaaattctgg attaaagcca cttcttacta atgctgggta tctatatttt
     3601 gaagcaaagc ttagttcagg gttttcaaca gtagcaattc tcgtatttct atagaataat
     3661 aattcatcat ttctattttt cacttctcca ccatacttca agtcattcgc aataactgac
     3721 cagtgtacat gccagttatt atttttaccg ctggcaattg tgtcataatt ttgctgatta
     3781 taactaatcg ttttggagta gctatttgat gaagttcgtc caataccttt cgttgaatcg
     3841 aatttaccac ctgagctata tgaaaatgta ctgtctactt tcgcagttga aattttattt
     3901 ttcggcaatt ggtctaatat ttcagtttta ggatttcttt ttacttgaaa atctacatgg
     3961 tactcacttg gatactttaa ccaatttgaa tttttttctt ctttatgaga ttcaaacttt
     4021 aaatttgaat gaattgagcc ttgtttttta acaagtaata cattcttgtc ataagttgga
     4081 tcatcgataa agtcgaattg taaattttgg agtatatttg ttttctcatc atatacagtt
     4141 tcagttcgtt ttgtgatttt accgtttttc ccaatatcat caggtactgt tgaattttta
     4201 tctttattag taacattacg tttctctttt tgttgagact tatcaacatg ttctttctta
     4261 ttttggtctt gcgagtcttt atgagctgaa tttgcttgag tcgttgctgc tgataacaat
     4321 aaaattgcac atgataatga tgacgctatt aaaacacgtt ttttattttt cattgttata
     4381 accttctttc gtatgattga tatttgttga tatgtatc
//
LOCUS       DBNENW010000171         4322 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNENW010000171 DBNENW010000000
VERSION     DBNENW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.174"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..336
                     /locus_tag="AC1LW8_002399"
     CDS             <1..336
                     /locus_tag="AC1LW8_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP4774188.1"
                     /translation="YTTHVDNNDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKPDH
                     SNKVKPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTGMKIITSWITWVFIGILGLY
                     LILRKRFNS"
     gene            complement(497..961)
                     /gene="bstA"
                     /locus_tag="AC1LW8_002400"
     CDS             complement(497..961)
                     /gene="bstA"
                     /locus_tag="AC1LW8_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HIP4774189.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKILDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVTGKENIDLNIYRPLFGNGSSPDEWKGEVPSIGTILEGLQTLPER
                     ARKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            complement(1065..1703)
                     /gene="pcp"
                     /locus_tag="AC1LW8_002401"
     CDS             complement(1065..1703)
                     /gene="pcp"
                     /locus_tag="AC1LW8_002401"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIP4774190.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLENIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(1855..2811)
                     /locus_tag="AC1LW8_002402"
     CDS             complement(1855..2811)
                     /locus_tag="AC1LW8_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIP4774191.1"
                     /translation="MNYQVILYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVNINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            3055..4026
                     /locus_tag="AC1LW8_002403"
     CDS             3055..4026
                     /locus_tag="AC1LW8_002403"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIP4774192.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNIQDIIEDFSTTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFRTVTPIIQNISVANGIALSKDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPDTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
BASE COUNT         1472 a          721 c          701 g         1428 t
ORIGIN      
        1 tatacaacac atgtggataa caatgatatg ggtaacttga ttgtgacgaa taaatatacg
       61 ccagaaaaac cgaataaacc aatctatcct gaaaaaccaa aagacaaaac accaccaact
      121 aaacctgatc actctaataa agttaaacca actcccccag ataagccatc aaaagtggat
      181 aaggatgatc aacctaaaga taataaaacc aaacctgaaa atcctctaaa agaattacca
      241 aaaactggta tgaagattat aacttcatgg attacatggg tatttatagg tatattggga
      301 ttgtatttaa ttttaagaaa aagatttaac tcataaacca ttataattat ttttatagat
      361 aaggctattc ctagttctat gtataataca tgatataaat aggtcacttt taatctgtat
      421 gtaagcagac taaaagtggc cttttaaaca aataaaaaaa ccgttaacgt gttgttaacg
      481 gttaataagc taatatttat tttaaatttt ttaatatacg agacatttct tcaattttac
      541 cagcatgaag tgggatatga atggcattta atactaataa ctcttctaag ttattacaac
      601 caacaattgg ttgtttcaat tcaattgcta aatcatcttc agttagtttt cgtgcacgtt
      661 caggtaatgt ttgaagacct tctaaaatcg ttccaatact cggtacttcg cccttccatt
      721 catctggaga cgaaccattt ccgaataaag gtctatatat atttaaatca atattctctt
      781 taccagtaac agctaaggct gattcaaaaa ttgttaatac atgcccaaat tgccaatgaa
      841 tggtattaga aaaaggctta tgataatcat caagtatctt ttcaactttc caagtatcat
      901 acacatttac taaatatcct aaacccatat caatgacatc aaataccgtt tttgtagtca
      961 ttattaattc ctccccccat aaataaaaca tacttccgta agtattaccc tattaacaaa
     1021 gttgttagtc atttaaggaa tgtattttat tggtgtactt ataactattc cgttgtgccc
     1081 agggctaggt gtaaatcctc atcattagaa atagcttcaa tggctgcagt caaacctgca
     1141 actatatttt ctaatggcat agatggtgta tcgggtttac caatgacctg ctctggtatg
     1201 tatggcacat gaataaatcc gaatcgtagg tgagggtaat gcttatcttg taaataacct
     1261 aagtgataaa gtgtgtgatt acaaacaaat gtacctgcgc tatttgaaag tgctccagga
     1321 agtccttgat taataatact ttgagtcatt gctttaactg gtaaatttga aaaataagct
     1381 ggcgcaccgt ctaagtgaat ggcttgatca ataggttgaa aatcatcatt atctggaata
     1441 cgtgcatcat caatattaat ggcgacacgt tctggggtaa tggcatttct accaccagct
     1501 tgtcctatag ctagtacaac atcataatga ttagatgcca acgttttatt tataatatta
     1561 tctactttct taaaagaggt tggtagtttt aatttatcga ttgtatgtgt gccaataata
     1621 tcttctagtt gagtcacagc ttcccatgag ggattgatat tttgattgtc aaaaggcgca
     1681 aaccctgtta ctaaaatgtg cattttaaga caccccgcag tattaaaatt taatttagta
     1741 taacacgcgc ataattaaaa acatatcagg ttatgtatgc gcataaaaaa cagccttgaa
     1801 aaacctttat aaatcggatt ttcaaagcgt gttttgaaag gtatttaatt aaaactaagc
     1861 atgttgatgt aaatcatcaa agtttgttaa acgttgttgc cactcattat cactaatatt
     1921 atttgcttga acataacgat tacgctcatg tttagcacat tcataagagc atgcacctaa
     1981 atatttagtt tcgttttctt cagaaactaa tatttgttta ttacattctg ggttagcgca
     2041 attaatataa cgttcacatg gtttgccatc aaaccaatcc ttaccaataa ttgttttttc
     2101 aacttggttg atattaacac tgatgcggtc atcaaatacg tacattttac cgtcccaata
     2161 ttcacctttt gtttcaggat ctttaccata tgtagcaata ccgccatgaa gttgtgctac
     2221 atcttcgaaa ccttctttta aaagccatcc agaaaatttt tcgcatcgaa ttccaccagt
     2281 acagtacgta accacttttt tatctgcaaa taacgcttta ttctctttaa tccagtcagg
     2341 caaatctcta aaacgtgtga tatttggacg aattgcacct cggaaatgac ctaaatcaaa
     2401 ttcataatca ttacgtgcat caataatgac tgtgtcatca tcttcaagag cttttctaaa
     2461 ttctacaggt gataaatatt ggccagttgt gtgtcttgga tcgacgtcat cttctaaatc
     2521 taaagcaacg atttcttttc gaggacgtac atgcattttc ttaaaagcat gtccttcagc
     2581 ttcatcaatt ttaaacacca tatctttgaa tcgttcatcg gcatgcatat gtgccatata
     2641 ttgttcggtt tcttcttttg taccagataa tgtgccgtta ataccttctg tagaaacaag
     2701 aattctacct tttaaatggt gtgctttaca aaaggctaag tgatcctgag caaactgttc
     2761 agggtcatca atcgtcgtat atttataata taaaataact tgatagttca taggttaatc
     2821 tccttctaaa aacatgttat aagcatgtta atttttaaaa taaagcgtca atacgcatat
     2881 ttatctacgt ccatcatttt aacagagttg tattgtcatt caatatttct agcttgaaat
     2941 cagccactag accgataaaa tagataaatg aaaccgttat cattttattt gatagttatc
     3001 ttgtttaaaa tgaaaatgat agttaaaacg aatgaaatga aaggaatgat catgatgtca
     3061 caacaagatt tacctacatt attttatagc gggaagtcca atagtgctgt tccaattata
     3121 tctgaaagtg aattacaaac aattacagct gaaccatggc ttgaaatttc caaaaaagga
     3181 ttgcagctag aaggattgaa ctttgatcgg cagggacaac tctttttatt ggatgtattc
     3241 gaaggcaata ttttcaaaat caatcctgaa acgaaggaaa tcaaacgacc ttttgtaagc
     3301 cataaagcga atcctgcagc aatcaaaata cataaagatg gccgattatt cgtttgttat
     3361 ttaggagatt ttaaatctac aggaggcatt tttgcagcta cagaaaatgg tgacaacata
     3421 caagatatta ttgaagattt ttcaacaact tattgtattg atgacatggt atttgattct
     3481 aaaggtggat tttattttac agattttaga ggatactcta ccaatccact agggggcgtt
     3541 tattatgttg cgccggactt tagaacagtg acgcctatca ttcaaaatat tagcgtagca
     3601 aatggtattg ctttaagtaa agatgaaaaa gtactatggg taacagaaac tacagccaat
     3661 cgattacatc gcattgcact tgaagatgat ggtgtgacga tacaaccatt tggagctacc
     3721 ataccgtact attttacagg tcatgaagga ccagactcat gttgtattga tagtgacgat
     3781 aatttatatg tggcaatgta tggtcaaggc cgagtattag tttttaataa aaggggttat
     3841 ccaataggac aaatattgat accaggccga gatgaagggc atatgttacg ttctacgcat
     3901 ccgcaattta tacctgacac aaatcaactc atcatttgtt ccaatgatat agaaatgggc
     3961 ggaggatcta tgctttatac agttaatggc tttgcaaaag gtcatcaaag ttttcagttt
     4021 caataagacc tacctgaatg aaaggcgtct aaatataagt ttaaatctta gcgattttta
     4081 agttagaagt taaatataat ttgcttcaat ttgtaaaatg aagttttgta taacgtaaat
     4141 aggcaatatt taatataaga aagtataaag aagaattttc ttgattattt ctaatgaaat
     4201 caatgccaat ttaataagga gttgtttatt ttggctattt atattttttg tacaatacta
     4261 gttttagttg cagttggagc acttttgaca aatagaataa gggataaaaa agataaaagg
     4321 ct
//
LOCUS       DBNENW010000172         4284 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNENW010000172 DBNENW010000000
VERSION     DBNENW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.175"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            96..196
                     /locus_tag="AC1LW8_002404"
                     /pseudo
     CDS             96..196
                     /locus_tag="AC1LW8_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            180..3053
                     /gene="fnbB"
                     /locus_tag="AC1LW8_002405"
     CDS             180..3053
                     /gene="fnbB"
                     /locus_tag="AC1LW8_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HIP4774193.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATERKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPTTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDTAQPSNVSEIKPRMKRSTDVTAVTEKEVVEEAK
                     ATGTDVTSKVEVEEGSEIVGHNNKETNVVNPHNAERVTLKYKWKFEDGIKPGDYFDFT
                     LSNNVETHGISPLRKVPDIKSKDDNILAVGKVMDERKIRYTFTDYINNKNNLMAELNL
                     NLFIDPTTVTKQGKQTVEVKLGENKISKEFDIKYLDGVKDNWGVTVNGRITLLDKENS
                     KIHHLAYINPKKSDMTSITINGFFAKGGLYTGNVPTVKVYEYLRSDELPESVYANTND
                     QEKFKDVTNDMSDKLTLSENGSYKLTLDALNKKSYVVSFEGKYNENDKELLFRTNLHG
                     YHANYGYYYYYPVSLTWDNGVAFYSNNAQGDGKDKPNDPIIEKSEPIELDIKSEPPVE
                     KHELTGTIEESNDSKPIDFEYHTAVEGVEGHAEGIIETEEDSIHVDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVITESNLVEFDEEPTKGILTGAVSDHTTVEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEK
                     DKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PQVSGHNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGILFGGLFSILGFALLRRNKKNHKA"
     gene            3233..4099
                     /gene="galU"
                     /locus_tag="AC1LW8_002406"
     CDS             3233..4099
                     /gene="galU"
                     /locus_tag="AC1LW8_002406"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIP4774194.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
BASE COUNT         1707 a          637 c          847 g         1093 t
ORIGIN      
        1 tgtatgcaat atatatgtga gtttcaaata ataaaaaact cattatacat aaagttaaca
       61 acaatcttaa ctttttatta actcgctttt tttcattgct tttaaaaacc gaacaatata
      121 gaattgcatt tattgagtta aaaaataaat gaattttaca tttaagggag aatattatag
      181 tgaaaagcaa tcttagatac ggcataagaa aacacaaatt gggagcggcc tcagtattct
      241 taggaacaat gatcgttgtt ggaatgggac aagaaaaaga agctgcagca tcggaacaaa
      301 acaatactac agtagaggaa agtgggagtt cagctactga aagaaaagca agcgaaacac
      361 aaacaactac aaataacgtt aatacaatag atgaaacaca atcatacagc gcgacatcaa
      421 ctgagcaacc atcacaatca acacaagtaa caacagaaga agcaccaaca actgtgcaag
      481 caccaaaagt agaaacttcg cgagttgatt tgccatcgga aaaagttgct gataaggaaa
      541 ctacaggaac tcaagttgac acagctcaac caagtaacgt ctcagaaatt aaaccaagaa
      601 tgaaaagatc aactgatgtt acagcagtta cagagaaaga agtagtggaa gaagctaaag
      661 cgacaggtac agatgtaaca agtaaagtgg aagtagaaga aggtagtgaa atagtagggc
      721 ataacaataa agagacaaat gttgttaatc ctcataatgc agaaagagta accttgaaat
      781 ataaatggaa attcgaggat ggtattaaac cgggggacta ctttgatttc acattaagta
      841 ataatgtaga aactcatggt atctcgcctc ttcgtaaagt tccagatata aaaagtaaag
      901 atgataatat tttggctgtt ggcaaagtga tggatgaaag aaaaattaga tatacgttta
      961 ctgattatat aaataacaaa aataatttaa tggctgaatt aaacttaaat ttgtttattg
     1021 atccaactac agttacgaag caaggaaagc aaactgttga ggttaaatta ggtgaaaata
     1081 agatttcaaa agaatttgat atcaaatatc tagacggtgt taaagataac tggggagtaa
     1141 ctgtaaatgg acgtataact ttattagata aagaaaatag taaaatacat catttagctt
     1201 atataaaccc taaaaaaagc gatatgacct caataactat aaatgggttt tttgctaaag
     1261 gtggattgta tacaggaaat gttccaacag tgaaagttta tgagtatttg agaagtgatg
     1321 aattaccaga aagcgtctat gctaacacaa atgatcaaga gaaatttaaa gatgttacca
     1381 atgacatgag tgataaatta acgctaagtg aaaacggtag ctataaatta actttagatg
     1441 cactaaataa gaaaagttat gttgtaagtt ttgaaggtaa atataatgaa aatgacaagg
     1501 agttattatt cagaactaat ttgcatggct atcatgctaa ttacggttat tactattatt
     1561 atccagtaag tttaacatgg gacaatggcg ttgcattcta ctctaataac gctcaaggcg
     1621 acggtaaaga taagccaaat gatcctatca ttgagaagag tgaaccaatt gaattagaca
     1681 ttaaatcaga gccgccagtg gagaagcatg aattgactgg tacaatcgaa gaaagtaacg
     1741 attctaagcc aattgatttt gaatatcata cagctgttga aggtgtagaa ggtcatgcag
     1801 aaggtattat tgaaactgaa gaagattcta ttcatgtgga ttttgaagaa tctacacatg
     1861 aaaattcaaa acatcacgct gatgttgttg aatatgaaga ggatacaaac ccaggtggtg
     1921 gccaagtaat aactgagtct aacttagttg aatttgacga agagcctaca aaaggtattc
     1981 taactggcgc agtgagcgac catacaacag ttgaagatac gaaagaatat acgactgaaa
     2041 gtaatctgat tgaactagta gatgaattac ctgaagagca tggtcaagca caaggaccaa
     2101 tcgaggaaat tactgaaaac aatcatcata tttctcattc tggtttagga actgaaaatg
     2161 gtcacgggaa ttatggtgtg attgaagaaa tcgaagaaaa tagccacgtt gatattaaga
     2221 gtgaattagg ttatgaaggt ggccaaaata gcggtaacca gtcattcgag gaagacacag
     2281 aagaagataa acctaaatat gaacaaggtg gcaatatcgt agatatcgat ttcgatagtg
     2341 tacctcaaat tcatggtcaa aataaaggtg atcagtcatt cgaagaagat acagagaaag
     2401 acaagcctaa atatgaacat ggtggtaata tcattgatat cgacttcgac agcgtgccac
     2461 atattcatgg attcaataag cacactgaaa ttattgaaga agatacaaac aaagataaac
     2521 caaattatca attcggtgga cacaatagtg ttgactttga agaagataca cttccacaag
     2581 taagtggtca taatgaaggt caacaaacga ttgaagaaga tacaacgccg ccaacaccac
     2641 caacaccaga agtaccaagt gagccggaaa caccaacacc accgacacca gaagtaccaa
     2701 gtgagccgga aacaccaaca ccgccaacac cagaggtacc aagtgagccg gaaacaccaa
     2761 caccaccaac gccagaggta ccagctgaac ctggtaaacc agtaccacct gccaaagaag
     2821 aacctaaaaa gccttctaaa ccagtggaac aaggtaaggt agtaacacct gtcattgaaa
     2881 tcaatgaaaa ggttaaagca gtggcaccaa ctaaaaaagc acaatctaag aaatctgaac
     2941 tacctgaaac aggtggagaa gaatcaacaa acaaaggtat actgttcgga ggattgttta
     3001 gtattttagg atttgcatta ttacgcagaa ataaaaagaa tcacaaagca taatcaatct
     3061 aaaattgaca ggtttatttc ataaattata tgaagtaagc ctgtttttta aaattaaatc
     3121 aaatttccca agaaataatt acatacttaa tgacactatg aaggcgttct aattagtgtt
     3181 aaaatgacgt tgatacatag atttaatact taggaaaagg agcacattaa ctttgaaaaa
     3241 aataaaaaag gcaatcattc ccgctgctgg tttagggact agatttttac cagcaactaa
     3301 agcgatgcca aaggaaatgc ttcctatctt agataaaccc acaatacaat atatcgttga
     3361 agaagctgca agagctggaa ttgaagatat tattatagtg acaggtcgcc acaaacgcgc
     3421 gattgaagat cattttgata gtcaaaaaga attagaaatg gtgttaaaag aaaaaggtaa
     3481 atctgagtta ctagaaaaag ttcagtattc aacagaactt gcgaatattt tttatgtaag
     3541 gcagaaagaa caaaaaggtt tagggcatgc gattagttct gcgcgtcaat ttatcggcaa
     3601 tgaaccattt gcggtattat taggtgacga tattgttgaa tctgaagtgc cagcagtgaa
     3661 acagttgatt gacgtttatg aagaaacagg gcactcagtt ataggtgtcc aagaagttcc
     3721 ggaagcagat actcatcgtt atgggattat tgacccatta actaaaaacg gacgtcagta
     3781 tgaagtgaaa aagtttgttg aaaagccagc acaaggtacg gcaccatcaa acctagctat
     3841 tatgggacgt tatgtattaa cgcctgaaat tttcgattat ttaaaaacgc aaaaagaagg
     3901 cgcaggtaac gaaattcagt taacagatgc gattgaacgt atgaataatg ataaccaagt
     3961 gtatgcatat gacttcgaag gcgaacgtta tgacgttgga gaaaaattag gcttcgttaa
     4021 aacaacgata gaatatgcat tgaaagatga cagtatgcga gaagaattaa cacgatttat
     4081 taaagaatta gggttataac attaaaaagc atgatactca ataaaattgg aacacgaaat
     4141 tgtgaactaa attgaatgag tgtcatgctt aatttatgtg ttgtgatata tagtagggta
     4201 ttgaaataat ataaaaacaa aagaaagtta attatgtatt tttatttata ctaaattgag
     4261 aagtattatg attgcttatt tatt
//
LOCUS       DBNENW010000173         4269 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNENW010000173 DBNENW010000000
VERSION     DBNENW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.176"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            85..768
                     /locus_tag="AC1LW8_002407"
     CDS             85..768
                     /locus_tag="AC1LW8_002407"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4774195.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(884..1546)
                     /locus_tag="AC1LW8_002408"
     CDS             complement(884..1546)
                     /locus_tag="AC1LW8_002408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIP4774196.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(1539..2156)
                     /locus_tag="AC1LW8_002409"
     CDS             complement(1539..2156)
                     /locus_tag="AC1LW8_002409"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIP4774197.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2223..2693
                     /gene="tadA"
                     /locus_tag="AC1LW8_002410"
     CDS             2223..2693
                     /gene="tadA"
                     /locus_tag="AC1LW8_002410"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIP4774198.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2840..3709
                     /locus_tag="AC1LW8_002411"
     CDS             2840..3709
                     /locus_tag="AC1LW8_002411"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP4774199.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3730..>4269
                     /locus_tag="AC1LW8_002412"
     CDS             3730..>4269
                     /locus_tag="AC1LW8_002412"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIP4774200.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVN"
BASE COUNT         1555 a          613 c          749 g         1352 t
ORIGIN      
        1 atactacgag ttcattttca gggaaataac gaacatcata gaatcagcat aatacgaaat
       61 aaataaatag gagtattgat attaatggaa tggatattat ttgataaaga tggcacgtta
      121 attgaatttg atagaagttg ggaaaaaata ggggtacgat ttgtacaatc attgcttgag
      181 actttcccag tacataataa agaagctgct ttaagacaac tcggtgtcat taaagaatct
      241 attgatccaa aatcagtgat gggttcagga tctttacaac aaattatcca ggcatttaat
      301 gatgtgacgg gacaagatac aaccgactgg tccaagtcaa caagtcaaaa gctggtagat
      361 gaacgtattc ctgaaattaa ttgggtagaa ggtgttaaag aagcacttat cgatttgaaa
      421 gcaaaaggct atcaacttgg tattgttacg agtgatacta aaaaaggtgt agaacaattt
      481 ttagcacata ccaatgctac ctcgttgttc gatttgatca tttctaccga agcggatgcc
      541 tatgagaagc caaatcctaa agtattatcg cctttatttg agcaatataa tgtagatcct
      601 cagaaagtag ctatagtagg agacactgct aatgatatga agacagcaag taatgcaaat
      661 ttaggtatgg caataggtgt attaacaggt attgcaacaa aagaagaatt acatgaagct
      721 gatattattt taaatagtgc ggcagatatt ttagaagctt taaattaaaa agaaagacat
      781 agtgtatatg tgaattaatg tattcgatag aattgataat gtaatcgatg atatctatgg
      841 aatatacatt gaatataaac atatacgcta tgtctttagt cttttatcgt gtatctactt
      901 gtcgatatgt ttgaataatt cgagcaattt tgtctatcat aggatttaaa gattcggggt
      961 ccttatggat atcatattca ttaatattga tacgtacaac tggacatgca ttaaagctat
     1021 taatccaatc gtcatagcgt ttaaatagct ttttccagta ttcagggtct gtattaattt
     1081 ccatttcgcg accacgttca ataatacgat caatgacctc atcatagtta cattctaaat
     1141 aaatcattac atcaggttta ggaaaataag gtgtcatgac catggcatta aataagtctg
     1201 aatatgtttt gaaatcttct ttactcattg tgccttcttc ttcatgcatt tttgcaaaaa
     1261 tatcaacatc ttcataaatt gatcgatctt ggacaaagcc accaccatat tcaaacatac
     1321 gcttttgttc tttaaaacgt tcagctaaga agtaaatttg caaatggaaa ctccatcgtt
     1381 caaaatcgct gtaaaattta tctaaatatg gattatgttc gacattttca aaagacgttt
     1441 taaagtttaa tttatctgca agtgcttgcg ttagtgttga ttttccaaca ccaactgtac
     1501 ctgcaatggt tataatggca ttttgtggaa taccgtaatt attcattggt aatatctcct
     1561 atcataggta atataatatg taatatatct tcgtaatctt gttcattttt aagaaaatca
     1621 atagaagttg tatcgattaa aactacattt gaaccattac tttgtaagga ctcataatac
     1681 tcacgataat ctttttttaa ctttaacaga tattcatctt ctatttgatg ctcaaaacta
     1741 cggttacgtt tagcaattct agattttaac acatcaaggt ctgcatctaa aaagataatc
     1801 atattcggca taatcatatc ttcagttaaa atatcataaa ttttactgaa tttctgaaat
     1861 tcaacagaac tcaaagtatt tttagcaaat atcttatttt tatgtatatg ataatcacta
     1921 actacacctt gatttagttg tgttacatct tgaaattgct tatatctatt gcataaaaag
     1981 aacatttcag tttgaaaact ccatttagag atatcttcat aaaagtctga taaaaatgga
     2041 ttttctgtga tgatttcttt ttcttcataa aaatctaaag tttgacttaa tttgtgtgca
     2101 agtgaagatt tacctacgcc aataggacct tcaattgcta taaaaggttt gttcatattc
     2161 acatctccaa atgttaaaat agacattgag tattgtacca taagtgaggt atctggacat
     2221 aaatgacaaa tgatatatat tttatgacat tagcgattga agaagctaaa aaagcagctc
     2281 aactaggcga agtacctata ggtgctatca tcactaaaga tgatgaagtt atcgctagag
     2341 cacataattt aagagaaaca ctacaacaac caacggcgca tgctgaacat attgcaattg
     2401 aacgtgcagc caaagtgtta ggtagttggc gtttagaagg ttgcacatta tatgtaacct
     2461 tagaaccatg tgtcatgtgc gcaggaacaa ttgtaatgag tcgcattcca agagtcgtct
     2521 atggcgcaga tgatcctaaa ggtggttgta gtggcagttt aatgaattta ttgcaacaat
     2581 ctaattttaa tcatcgtgca attgttgata aaggtgtact taaagaagca tgtagcacat
     2641 tattaacaac attttttaaa aacttaagag ccaataagaa atccaccaat tagacgatag
     2701 tacatactaa ttaaaatttg ttagaattac attatatgat aaattaatga caatataaat
     2761 gttaatagaa aagtatgtgc gctgagtata tattcttatt taagaacaag attttaaatt
     2821 tacgaaacga ggtaacataa tgataaaact aatagccact gatatggatg gcacgctact
     2881 taatgcagca catgaaattt ctcaacctaa tattgatgcg attaaatacg ctcaagaaca
     2941 agggataacg gttgttatcg cgacaggtcg agcattttat gaagcacaag caccagttgc
     3001 tgacacagat ttaacagtac catatatttg tttgaatggt gctgaagtac gtgatgaaac
     3061 tttcaatgta atgagcactt cacaccttaa taaatcgcta gtacacaaaa ttacaaatgt
     3121 tttaaaagat gcaggtattt attatcaagt atacacgagt cgtgcgattt atactgaaga
     3181 tccacaaaga gatttagaca tttacataga tattgctgag cgtgcaggtc aacatgcaaa
     3241 cgttgagcgt attaaaaatg gtattcaaag acgcatagat aatggtacgt tgaaagttgt
     3301 tgataattat gatgctattg aaaacatacc tggtgaatta attatgaaaa tattagcatt
     3361 tgatggaaat ttagaaaaaa ttgacaaagc tagtaaaatt ttagctgaat ctccgaattt
     3421 agctatatca tcatcttcga gaggaaatat agaaataacg cattcagatg cacaaaaagg
     3481 tattgcgcta gaaacaattg ccgaaagatt agggattgaa atgaaagaag tcatggcaat
     3541 aggtgacaat ttaaatgact tatcaatgtt agagaaagtt ggctatccag ttgcgatgga
     3601 aaatggtgca gaagaagtta aaaaaatagc gaaatatgtc acagatacga atgaaaatag
     3661 tggtgttgga aaagctatta tgaaattatt acgtgaacaa caagtttaat aaaaaaagag
     3721 gggtcaaata tgaaaggatt aattattatt ggcagtgcac aagtgaattc acatacaagt
     3781 gcactagcaa gatacttaac tgagcatttt aaaacacatg atattgaagc ggaaatattc
     3841 gatttagcag aaaaaccgtt aaatcaatta gatttttcag gaacaacacc gtctattgat
     3901 gaaatcaaac aaaatatgaa agatttaaaa gagaaagcaa tggcggcgga ctttttaata
     3961 ttaggaacgc caaactatca tggttcatat tctggaatat tgaaaaatgc attagatcat
     4021 ctaaatatgg attattttaa aatgaaacct gtaggcttaa taggaaatag tggtggtatt
     4081 gttagttcag agccattgtc acatttaaga gtaatcgtca gaagtttact aggcattgct
     4141 gtaccaactc aaatagcaac acatgattct gattttgcta aaaatgaaga tggttcatat
     4201 tacttaaatg atagtgaatt ccaattacga gcaagattat ttgtcgatca aattgtatct
     4261 tttgtgaat
//
LOCUS       DBNENW010000174         4265 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNENW010000174 DBNENW010000000
VERSION     DBNENW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.177"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..828
                     /locus_tag="AC1LW8_002413"
     CDS             <1..828
                     /locus_tag="AC1LW8_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HIP4774201.1"
                     /translation="SGLAGIGRYLLNRVDENEFNVKALKSILVYFKDIQYSKNSWVVP
                     QESQFLESDKNYFTEGNINLGLAHGVLGPMSLFALCVIKGITIENHQHILKDMYKFIM
                     DEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNTGVMTTLFLIGQALQDDEIIKMSK
                     KVMLQVVNDKDENLISPTICHGLSSQILMLTIMNLNFELNEVSDYITVLINKLISHYK
                     EDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLTLMTLKNTKILNEKNWTNAFLIS"
     gene            844..1362
                     /locus_tag="AC1LW8_002414"
     CDS             844..1362
                     /locus_tag="AC1LW8_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIP4774202.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            1372..2745
                     /locus_tag="AC1LW8_002415"
     CDS             1372..2745
                     /locus_tag="AC1LW8_002415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIP4774203.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            2768..3460
                     /locus_tag="AC1LW8_002416"
     CDS             2768..3460
                     /locus_tag="AC1LW8_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HIP4774204.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            3457..4218
                     /locus_tag="AC1LW8_002417"
     CDS             3457..4218
                     /locus_tag="AC1LW8_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HIP4774205.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT         1591 a          534 c          756 g         1384 t
ORIGIN      
        1 agtggattag ctgggatagg gagatatttg ctaaatagag tagatgagaa tgaatttaat
       61 gttaaagcat taaaaagcat attagtatac tttaaagata ttcaatattc taaaaatagc
      121 tgggtagtcc cacaagaaag tcaattttta gagtctgata aaaattattt tactgaaggt
      181 aatatcaatc ttggccttgc acatggagtg ctaggaccga tgtctttatt tgcattatgc
      241 gtgattaaag gaattacgat tgaaaatcat cagcacatat taaaagacat gtacaaattt
      301 atcatggacg aaaaattttg taaccacgaa agatggttgc agcgttacga tttaatttct
      361 gaacgtaatc atttcaattt tattcggaat ggttggtgtt atggcaatac gggtgtaatg
      421 acgacgttgt ttttaatcgg ccaagcatta caagatgatg aaataattaa aatgtctaaa
      481 aaagtgatgc tacaagtagt aaatgataaa gatgaaaatt taataagtcc aactatttgt
      541 catggattgt catcacaaat attaatgtta acaattatga atttgaattt tgaattaaat
      601 gaagtgtctg attatatcac tgtattaata aataaactga tttctcatta taaggaagat
      661 tatctggtga attttataga cattaatgaa aataagcaag atgtatttaa aagtaggaaa
      721 gttggccttt tagaaggtga attaggggtc tatctaacat tgatgacact gaaaaataca
      781 aaaattctca atgaaaaaaa ttggaccaat gcgtttttaa ttagctgaat ggaggcgtat
      841 atcatgggag aaaatgtatt gatttgtttg tgtggctcag ttaatagtat caatattagc
      901 cactatatta ttgaattaaa atcaaaattt gatgaagtaa atgtaatcgc gtcaacaaac
      961 ggtagaaagt ttattaatgg tgaaatatta aagcaatttt gcgataacta ttatgacgaa
     1021 tttgaagacc cttttttaaa tcatgtagat atagcaaata aacatgacaa gattattatt
     1081 ttacctgcga cttctaatac gattaataaa attgcaaatg gtatatgtga caatttatta
     1141 ttaactattt gtcatacagc ttttgaaaaa ctttctatat ttccaaatat gaatttacga
     1201 atgtgggaaa atccagttac tcaaaataat attcgattat taaaagatta tggtgtatca
     1261 atatatccag caaatatttc agaaagttat gaattagcgt caaaaacatt taaaaagaat
     1321 gttgtcgcac ctgaaccata taaagttctg gaattcattt gagattagaa tatgaaaatc
     1381 ataaagcgcg ctattattag tttaataata ttgagtcttt taatatctat tacaatgagt
     1441 aatgcatcag cgtcagaaga actatattac agtgttgaat acaaaaatac agcaacattt
     1501 aacaagttag ttaaaaagaa gtccttaaac gttgtctata atataccgga attacatgtg
     1561 gcacagatta aaatgacgaa aatgcatgct aatgctttag caaactataa aaatgatatt
     1621 aaatatatca atgccacatg ttcaacttgt attactagcg agaaaacaat agacagaaca
     1681 tcaaatgagt cattattttc aagacaatgg gatatgaata aaataaccaa taatggtgca
     1741 tcgtatgatg atttgccaaa acatgctaac accaaaatag caatcataga tacaggtgtg
     1801 atgaaaaacc atgacgattt gaaaaataat ttctcgactg attctaaaaa tttagtacct
     1861 ttaaacggtt ttagaggtac tgaaccggag gaaacaggtg atgttcacga tgtcaatgat
     1921 aggaaaggac atggcacgat ggtgtcgggt caaacgagtg ctaatggtaa gttaataggt
     1981 gttgcaccga ataacaaatt tacaatgtat cgcgtgtttg gtagtaaaaa aacagaactg
     2041 ctttgggtat caaaagcgat tgttcaagct gcaaatgatg gaaatcaagt cattaatatt
     2101 agtgttggta gttatattat tttggacaaa aatgaccatc aaacatttag aaaagatgaa
     2161 aaagtagaat acgatgcgtt acagaaagca atcaattacg ccaagaagaa aaaatctatc
     2221 gttgttgctg cagctggtaa tgatggtatt gatgtcaatg acaaacagaa actaaaatta
     2281 cagcgtgaat atcaaggtaa tggcgaagtg aaagatgttc ctgcatctat ggacaatgtc
     2341 gttacagtag gatctacaga tcaaaagagt aatctatctg agttttccaa ttttggtatg
     2401 aattatacag atattgctgc gcccggagga tcatttgctt atttaaatca attcggtgtg
     2461 gataaatgga tgaatgaagg gtatatgcat aaggagaaca ttttaactac tgccaataac
     2521 ggaagatata tttatcaagc tggaacttca ttagccacac ctaaagtttc gggagcacta
     2581 gctttaatca ttgataaata tcatcttgaa aaacatccag ataaagcgat tgaattgtta
     2641 tatcagcatg ggacatctaa gaataataaa ccatttagta gatatgggca tggtgagctt
     2701 gatgtgtata aagcattaaa tgtagcaaat caaaaagcaa gttaataaat caaaggagtt
     2761 tttgattatg gcaaaattag ttactgaaaa catttcgaaa cggtttaaaa atcaagatgt
     2821 attaaagcat attaatatca ctttagaaaa taacgaagtt tatggattac ttggtattaa
     2881 tggagccggt aaaacgacac ttatgaaaat tatatgtggc atacttcaac aagattcagg
     2941 ggaaattaaa ttagataata gaccaatgac acgaaatgat ttgcacaaag ttggttcgct
     3001 tattgaaaca cctgcgacat ataatcattt aagtgcacaa gataatttga aaattgtgtg
     3061 tttaaatgaa agcgttgatt tcagcgaaat taatagtgtt ttaagcttag tcaatttaaa
     3121 tgtcgataaa aagaaaaagg ttaaggactt ttctttaggt atgaaacaaa gacttggaat
     3181 tgcaatggca ttaattaaaa agccagaaat tttagtatta gacgaaccat ctaatggttt
     3241 agacccatat ggaatccaag aacttagaga acttctaaaa ttattaacag aacaaggtac
     3301 tagtattatt atttcaagtc acattttatc tgaaatccaa gttttagcag atcatatcgg
     3361 tattattcat gagggtgagc taaaatatca gcaaagaaat aacaaagatg aaaacttaga
     3421 agagatattc ttcaaaataa cgaaaggtga ttacaaatga tacatttaaa gatagaaggt
     3481 atcaaattta aaaattcttt cagtatgtat gttttattaa taagtccgct ggtatttctt
     3541 tgttttgcta ttttcacagt cttattcgcc aaaagtaata cgggaacagc gaatagtgtg
     3601 tcaccatata taactttact atttaatatt tggccaattg ctttcatccc gattgtatta
     3661 tgtatggctt gtaattcgtt atttaaaatt gaaatgagaa ataaatcatt taattattac
     3721 ttaagtaata attggtcgat tacaaaagaa ataagagcaa agattttcat tttatcaata
     3781 gcatttttgg tacattgctt tttagtattt attattgctt atataggtga cctaattatt
     3841 aatccacatc cggttaatgc tatgttgtta ttggtgacaa tattgttgat gtatgtagta
     3901 tctctaccat tgataccgct caacttttta ttaactcgat actttggtgt gttcgtatca
     3961 atattaataa acttagtatt atcagtcatt tgtgtcttgt ttttaacatt gaagagttta
     4021 ttttgggtgt tgccgtgggg gataatgcag agaatcccgc ttattacgct tggtatacta
     4081 cctaatggtt tagttgtaaa ccataattca aaatacttta atgatctcaa tgccttatat
     4141 atttcgatta ttgttagcat cattattttc gcgatagtaa catttttaaa taataagaaa
     4201 agttggcgat taaaatgata attaacgaat taaagtcatg taagttgaaa ttttctaagc
     4261 aagcg
//
LOCUS       DBNENW010000175         4247 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNENW010000175 DBNENW010000000
VERSION     DBNENW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.178"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            33..4136
                     /gene="sdrD"
                     /locus_tag="AC1LW8_002418"
     CDS             33..4136
                     /gene="sdrD"
                     /locus_tag="AC1LW8_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HIP4774206.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNAQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLE
                     LKSQIKVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKEVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTTSESPTLVQMVTLSSDNSKSASMGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTT
                     KDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1727 a          654 c          776 g         1090 t
ORIGIN      
        1 atttaaaaat ttgatgaatt aggagtaatc taatgctaaa cagagaaaat aaaacggcaa
       61 taacaagaaa aggcatggta tccaatcgat taaataaatt ttcgattaga aagtacacag
      121 tgggaacagc atcaatttta gtaggtacaa cattaatttt tggtctgggg aaccaagaag
      181 caaaggctgc agaaagtact aataaagaat tgaacgaagc gacaacttca gcaagtgata
      241 atcaatcgag tgataaagtt gatatgcagc aactaaatca agaagacaat actaaaaatg
      301 ataatcaaaa agaaatggta tcatctcaag gtaatgaaac gacttcaaat gggaataaat
      361 caatagaaaa agaaagtgta caatctacca ctggaaataa agttgaagtt tcaactgcca
      421 aatcagatga gcaagcttca ccaaaatcta cgaatgaaga tttaaacact aaacaaacta
      481 taagtaatca agaagcgtta caacctgatt tgcaagagaa taaatcagtg gtaaatgctc
      541 aaccaactaa tgaggaaaac aaaaaggtag atgccaaaac tgaatcaact acattaaatg
      601 ttaaaagtga tgctatcaag agtaatgctg aaactcttgt tgataacaat agtaattcaa
      661 ataatgaaaa taatgcagat atcattttgc caaaaagtac agcacctaaa cgtttgaata
      721 caagaatgcg tatagcagca gtacagccat catcaacaga ggctaaaaat gttaatgatt
      781 taattacatc aaatacaaca ttaactgtcg ttgatgcaga taaaaacaat aaaatcgtac
      841 cagcacaaga ttatctagaa ttaaaatcac aaattaaagt tgatgacaaa gttaaatcag
      901 gtgattattt cacaataaaa tactcagata cagtacaagt atatggattg aatccggaag
      961 atattaaaaa tattggagat attaaagatc caaataatgg tgaaacaatt gcgactgcaa
     1021 aacatgatac tgcaaataat ttaattacat atacatttac agattatgtc gatagattta
     1081 attctgtaca aatgggaatt aattattcaa tttatatgga tgctgataca attcctgtta
     1141 gtaaaaacga tgttgagttt aatgttacga taggtaatac tacaacaaaa acaactgcta
     1201 acattcaata tccagattat gttgtaaatg agaaaaattc aattggatca gcgttcactg
     1261 aaacagtttc acatgttgga aataaagaaa atccagggta ctataaacaa acgatttatg
     1321 taaatccatc ggaaaattct ttaacaaatg ccaaactaaa agttcaagct taccactcaa
     1381 gttatcctaa taatatcggg caaataaata aagaggtaac agatataaaa atatatcaag
     1441 ttcctaaagg ttatacatta aataaaggat acgatgtgaa tactaaagag cttacagatg
     1501 taacaaatca atacttgcag aaaattacat atggcgacaa caatagcgct gttattgatt
     1561 ttggaaatgc agattctgct tatgttgtaa tggttaatac aaaattccaa tatacaacta
     1621 gcgaaagccc aacacttgtt caaatggtta cattaagttc agacaatagt aaatctgcct
     1681 caatgggtaa tgctttagga ttcactaata accaaagtgg cggagctggt caagaagtat
     1741 ataaaattgg taactacgta tgggaagata ctaataaaaa cggtgttcaa gaattaggag
     1801 aaaaaggcgt tggcaatgta actgtaactg tatttgataa taatacaaat acaaaagtag
     1861 gagaagcagt tactaaagaa gatgggtcat acttgattcc aaacttacct aatggagatt
     1921 accgtgtaga attttcaaac ttaccaaaag gttatgaagt aaccccttca aaacaaggta
     1981 ataacgaaga attagattca aacggcttat cttcagttat tacagttaat ggcaaagata
     2041 acttatctgc agacttaggt atttacaaac ctaaatacaa cttaggtgac tatgtctggg
     2101 aagatacaaa taaaaatggt atccaagacc aagatgaaaa aggtatatct ggcgtaacgg
     2161 taacattaaa agatgaaaac ggtaacgtgt taaaaacagt tacaacagac gcagatggca
     2221 aatataaatt tactgattta gataatggta attataaagt tgaatttact acaccagaag
     2281 gctatacacc gactacagta acatctggta gcgacattga aaaagactct aatggtttaa
     2341 caacaacagg tgttattaat ggtgctgata acatgacatt agatagtgga ttctacaaaa
     2401 caccaaaata taatttaggt aattatgtat gggaagatac aaataaagat ggtaagcagg
     2461 attcaactga aaaaggtatt tcaggcgtaa cagttacatt gaaaaatgaa aacggtgaag
     2521 ttttacaaac aactaaaaca gataaagatg gtaaatatca atttactgga ttagaaaatg
     2581 gaacttataa agttgaattc gaaacaccat caggttacac accaacacaa gtaggttcag
     2641 gaactgatga aggtatagat tcaaatggta catcaacaac aggtgtcatt aaagataaag
     2701 ataacgatac tattgactct ggtttctaca aaccgactta caacttaggt gactatgtat
     2761 gggaagatac aaataaaaac ggtgttcaag ataaagatga aaagggtatt tcaggtgtaa
     2821 cagttacgtt aaaagatgaa aacgacaaag ttttaaaaac agttacaaca gatgaaaatg
     2881 gtaaatatca attcactgat ttaaacaatg gaacttataa agttgaattc gagacaccat
     2941 caggttatac accaacttca gtaacttctg gaaatgatac tgaaaaagat tctaatggtt
     3001 taacaacaac aggtgtcatt aaagatgcag ataacatgac attagacagt ggtttctata
     3061 aaacaccaaa atatagttta ggtgattatg tttggtacga cagtaataaa gacggcaaac
     3121 aagattcaac tgaaaaaggt atcaaagatg ttaaagttac tttattaaat gaaaaaggcg
     3181 aagtaattgg aacaactaaa acagatgaaa atggtaaata tcgtttcgat aatttagata
     3241 gcggtaaata caaagttatt tttgaaaagc ctgctggctt aacacaaaca ggtacaaata
     3301 caactgaaga tgataaagat gcagatggtg gcgaagttga cgtaacaatt acggatcatg
     3361 atgatttcac acttgataac ggatacttcg aagaagatac atcagatagc gactcagatt
     3421 cagatagcga ttcagattca gatagcgact cagactcaga ttcagacagc gactcagact
     3481 cggatagcga ttcagattca gacagtgatt cagattcaga cagcgattcg gattcagaca
     3541 gcgactcaga ctcggatagc gactcagact cagatagcga ttcagattca gatagcgatt
     3601 cggactcaga cagtgattca gattcagaca gcgactcaga ttcagatagc gattcagact
     3661 cagacagtga ttcagattca gatagcgact cagactcaga tagcgattca gaatcagaca
     3721 gcgattcaga cagcgactca gactcggata gcgactcaga ctcagacagc gattcagact
     3781 cggatagcga ttcagattcg gacagcgact cagactcaga cagtgattca gattcagata
     3841 gcgactcaga ctcagatagc gactcagatt cagacagcga ttcagactca gatagtgact
     3901 cagattcgga cagcgattca gactcagata gcgactcaga ttcagactca gatgcaggta
     3961 agcacacacc tgttaaacca atgagtacta ctaaagacca tcacaataaa gcaaaagcat
     4021 taccagaaac aggtaatgaa aatagcggct caaataacgc aacgttattt ggcggattat
     4081 tcgcagcatt aggatcatta ttgttattcg gtcgtcgtaa aaaacaaaat aaataataca
     4141 gcttatacca ggtccgtgag gcctggtttt ttatttcaaa atttatgaaa tgtatcttat
     4201 gtttgagatt aaatagtgca attttttaaa aaatataaca taaatca
//
LOCUS       DBNENW010000176         4188 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNENW010000176 DBNENW010000000
VERSION     DBNENW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.179"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..429
                     /locus_tag="AC1LW8_002419"
     CDS             <1..429
                     /locus_tag="AC1LW8_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774207.1"
                     /translation="KISFLPALGFNQILLEREEDQLNIMNSATEEHHHKDYIKLYNLG
                     GGAAKKIAIEVLLGNDKVIQKKYVNILPSKEGYMLPINKNVYEELERTIENNGYEADL
                     NVRMTYYHNVSRKQQEVILKGQIDCFNTYNNKEIYDLQFI"
     gene            568..1098
                     /locus_tag="AC1LW8_002420"
     CDS             568..1098
                     /locus_tag="AC1LW8_002420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774208.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1361..4171
                     /gene="clfA"
                     /locus_tag="AC1LW8_002421"
     CDS             1361..4171
                     /gene="clfA"
                     /locus_tag="AC1LW8_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HIP4774209.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSA
                     LTNATTEETPVTGEATTATNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVN
                     SPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLS
                     AVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNSAVKGDTFKI
                     TVPKELNLNGVISTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDTKENVTANITM
                     PAYIDPENVTKTGNVTLTTGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNT
                     YRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVN
                     PENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTL
                     YGYDSRFVWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGS
                     DSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSAS
                     DSDSASDSDSASDSDSDNDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSNSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDS
                     DSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIW
                     GLLASIGSLLLFRRKKENKDKK"
BASE COUNT         1526 a          669 c          834 g         1159 t
ORIGIN      
        1 aaaattagct ttctgccagc tttaggtttt aaccaaattt tattagaaag ggaggaggat
       61 caacttaata taatgaattc ggcaacagaa gagcatcatc ataaagatta tattaaacta
      121 tataatttag gtggcggtgc tgctaaaaaa attgcaatag aggttttatt gggtaatgat
      181 aaagtcattc agaaaaaata cgtgaatatt ttacctagta aagaagggta catgttacca
      241 attaataaaa atgtgtacga agaattagaa agaacgattg agaacaatgg ttatgaagct
      301 gatttgaatg tacgtatgac ttattatcat aatgtaagtc gcaaacaaca ggaagttata
      361 ttaaaaggtc aaatcgactg ttttaatact tataataata aagaaattta tgatttgcag
      421 tttatctaaa aattgattta agagggtagt tgtttattgc gaaaaatatc attcaatttt
      481 aatgaaataa tggcgtcatt actataaaat attactttat gttgtaatgc atttttctat
      541 aagatagaac taaaaggagg ggcaaagatg caaattagac aaatacatca acatgacttt
      601 gctcaagttg accagttaat tagaacggca tttgaaaata gtgaacatgg ttatggtaat
      661 gaatcagaac tagtagacca aattcgtcta agtgatacgt atgacaataa cttagaatta
      721 gtagctgttc ttcaaaatga agttgtaggg cacggtttac taagtgaagt ttatcttgat
      781 aacgaggcac aacgggaaat tggattagtg ttagcacctg tatctgttga tattcatcat
      841 caaaataaag gtattgggaa gcgattgatt caagcattag aacgagaagc aatattaaaa
      901 ggatataatt ttatcagtgt attaggatgg ccgacgtatt atgccaatct aggatatcaa
      961 cgcgcaagta tgtacgacat ttatccacca tatgatggta taccagacga agcgttttta
     1021 attaaagaat taaaagtgaa cagtttagcg ggaaaaacag gtaccataaa ttacacatct
     1081 gcttttgaaa aaatatgatt tcaagctagg attacattag gtagagttca tattaataat
     1141 aaaaaatgtt tgcaatgaaa tcgtgcattg tcgtttgcaa tttttaaaat agcaataaat
     1201 aaaatgtttg ttagtaaagt attattgtgg ataataaaat atcgatacaa attaattgct
     1261 ataatgcaat tttagtgtat aattccatta acagagatta aatatatctt taaagggtat
     1321 atagttaata taaaatgact ttttaaaaag agggaataaa atgaatatga agaaaaaaga
     1381 aaaacacgca attcggaaaa aatcgattgg cgtggcttca gtgcttgtag gtacgttaat
     1441 cggttttgga ctactcagca gtaaagaagc agatgcaagt gaaaatagtg ttacgcaatc
     1501 tgatagcgca agtaacgaaa gcaaaagtaa tgattcaagt agcgttagtg ctgcacctaa
     1561 aacagacgac acaaacgtga gtgatactaa aacatcgtca aacactaata atggcgaaac
     1621 gagtgtggcg caaaatccag cacaacagga aacgacacaa tcagcattaa caaatgcaac
     1681 tacggaagaa actccggtaa ctggtgaagc tactacggca acgaatcaag ctaatacacc
     1741 ggcaacaact caatcaagca atacaaatgc ggaggaatta gtgaatcaaa caagtaatga
     1801 aacgacttct aatgatacta atacagtatc atctgtaaat tcacctcaaa attctacaaa
     1861 tgcggaaaat gtttcaacaa cgcaagatac ttcaactgaa gcaacacctt caaacaatga
     1921 atcagctcca cagagtacag atgcaagtaa taaagatgta gttaatcaag cggttaatac
     1981 aagtgcgcct agaatgagag catttagttt atcggcagta gctgcagatg caccggcagc
     2041 tggcaaagat attacgaatc agttgacgaa tgtgacagtt ggtattgact ctggagatac
     2101 agtttatccg caccaagcag gctatgtcaa actgaattat ggtttttcag tgcctaattc
     2161 tgctgttaaa ggtgacacat tcaaaataac tgtacctaaa gaattaaact taaatggtgt
     2221 aatttcaact gctaaagtgc ctccaattat ggccggagat caagtattgg caaatggtgt
     2281 aatcgatagt gatggtaatg ttatttatac atttacagac tatgttgata ctaaagaaaa
     2341 tgtaacagct aatattacta tgccagctta tattgaccct gaaaatgtta caaagacagg
     2401 taatgtaaca ttgacaactg gcataggtag tacaacagca aacaaaacag tattagtaga
     2461 ttatgaaaaa tatggtaagt tttataactt atctattaaa ggtacaattg accaaatcga
     2521 taaaacaaat aatacgtatc gtcagacaat ttatgtcaat ccaagtggag ataatgttat
     2581 tgcgccggtt ttaacaggta atttaaaacc aaatacggat agtaatgcat taatagatca
     2641 gcaaaataca agtattaaag tatataaagt agataatgca gctgatttat ctgaaagtta
     2701 ctttgtgaat ccagaaaact ttgaggatgt cactaatagt gtgaatatta cattcccaaa
     2761 tccaaatcaa tataaagtag agtttaatac gcctgatgat caaattacaa caccgtatat
     2821 tgtagttgtt aatggtcata ttgatccgaa tagcaaaggt gatttagctt tacgttcaac
     2881 tttatatgga tatgactcaa ggtttgtatg gagatctatg tcatgggaca acgaagtagc
     2941 atttaataac ggatcaggtt ctggtgacgg tatcgataaa ccagttgttc ctgaacaacc
     3001 tgatgagcct ggtgaaattg aaccaattcc agaggattca gattctgacc caggttcaga
     3061 ttctggcagc gattctaatt cagatagcgg ttcagattcg ggtagtgatt ctacatcaga
     3121 tagtggttca gattcagcga gtgattcaga ttcagcgagt gattcagatt cagcaagtga
     3181 ttcagactca gcgagtgatt cagattcagc aagcgattca gattcagcga gcgattcaga
     3241 ttcagcgagt gattcagatt cagcaagcga ttccgactca gacaatgact cggattcaga
     3301 tagcgattct gactcagaca gtgactcaga ttccgatagc gattctgact cagacagtga
     3361 ctcagattca gatagcgatt cagattcaga tagcgattca gattccgaca gtgattccga
     3421 ctcagacagc gattctgact ccgacagtga ttccgactca gacagcgatt cagattccga
     3481 cagtgattcc gactccgata gcgattccga ctcagatagc gactcagatt cagacagcga
     3541 ttcagattca gacagcgatt cagattcaga tagcgattca gattccaaca gtgactcaga
     3601 ttccgacagt gactcggatt cagatagcga ttcagattcc gacagtgact cagattccga
     3661 cagtgactca gactcagaca gtgattcgga ttcagcgagt gattcggatt cagatagtga
     3721 ttccgactcc gacagtgact cggattcaga tagcgactca gactcggata gcgactcgga
     3781 ttcagatagc gattcggact cagatagcga ttcagaatca gacagcgatt cagattcaga
     3841 ttcagacagc gactcagatt cagatagtga ctcggattca gcgagtgatt cagactcagg
     3901 tagtgactcc gattcatcaa gtgattccga ctcagaaagt gattcaaata gcgattccga
     3961 gtcaggttct aacaataatg tagttccgcc taattcacct aaaaatggta ctaatgcttc
     4021 taataaaaat gaggctaaag atagtaaaga accattacca gatacaggtt ctgaagatga
     4081 agcaaatacg tcactaattt ggggattatt agcatcaata ggttcattac tacttttcag
     4141 aagaaaaaaa gaaaataaag ataagaaata agtaataatg atattaaa
//
LOCUS       DBNENW010000177         4185 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNENW010000177 DBNENW010000000
VERSION     DBNENW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.180"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(135..1196)
                     /locus_tag="AC1LW8_002422"
     CDS             complement(135..1196)
                     /locus_tag="AC1LW8_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIP4774210.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            complement(1744..1827)
                     /locus_tag="AC1LW8_002423"
     tRNA            complement(1744..1827)
                     /locus_tag="AC1LW8_002423"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1791..1793),aa:Leu,seq:caa)
     gene            complement(1866..1940)
                     /locus_tag="AC1LW8_002424"
     tRNA            complement(1866..1940)
                     /locus_tag="AC1LW8_002424"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1905..1907),aa:Gly,seq:tcc)
     gene            complement(2044..2118)
                     /locus_tag="AC1LW8_002425"
     tRNA            complement(2044..2118)
                     /locus_tag="AC1LW8_002425"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2083..2085),aa:Gly,seq:tcc)
     gene            complement(2126..2199)
                     /locus_tag="AC1LW8_002426"
     tRNA            complement(2126..2199)
                     /locus_tag="AC1LW8_002426"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2165..2167),aa:Cys,seq:gca)
     gene            complement(2205..2276)
                     /locus_tag="AC1LW8_002427"
     tRNA            complement(2205..2276)
                     /locus_tag="AC1LW8_002427"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2242..2244),aa:Gln,seq:ttg)
     gene            complement(2288..2360)
                     /locus_tag="AC1LW8_002428"
     tRNA            complement(2288..2360)
                     /locus_tag="AC1LW8_002428"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2325..2327),aa:His,seq:gtg)
     gene            complement(2363..2436)
                     /locus_tag="AC1LW8_002429"
     tRNA            complement(2363..2436)
                     /locus_tag="AC1LW8_002429"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2402..2404),aa:Trp,seq:cca)
     gene            complement(2452..2532)
                     /locus_tag="AC1LW8_002430"
     tRNA            complement(2452..2532)
                     /locus_tag="AC1LW8_002430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2496..2498),aa:Tyr,seq:gta)
     gene            complement(2538..2613)
                     /locus_tag="AC1LW8_002431"
     tRNA            complement(2538..2613)
                     /locus_tag="AC1LW8_002431"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2578..2580),aa:Thr,seq:tgt)
     gene            complement(2621..2693)
                     /locus_tag="AC1LW8_002432"
     tRNA            complement(2621..2693)
                     /locus_tag="AC1LW8_002432"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2658..2660),aa:Phe,seq:gaa)
     gene            complement(2712..2787)
                     /locus_tag="AC1LW8_002433"
     tRNA            complement(2712..2787)
                     /locus_tag="AC1LW8_002433"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2752..2754),aa:Asp,seq:gtc)
     gene            complement(2797..2870)
                     /locus_tag="AC1LW8_002434"
     tRNA            complement(2797..2870)
                     /locus_tag="AC1LW8_002434"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2834..2836),aa:Met,seq:cat)
     gene            complement(2885..2977)
                     /locus_tag="AC1LW8_002435"
     tRNA            complement(2885..2977)
                     /locus_tag="AC1LW8_002435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2939..2941),aa:Ser,seq:tga)
     gene            complement(3016..3091)
                     /locus_tag="AC1LW8_002436"
     tRNA            complement(3016..3091)
                     /locus_tag="AC1LW8_002436"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3056..3058),aa:Asp,seq:gtc)
     gene            complement(3102..3191)
                     /locus_tag="AC1LW8_002437"
     tRNA            complement(3102..3191)
                     /locus_tag="AC1LW8_002437"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3153..3155),aa:Ser,seq:tga)
     gene            complement(3201..3274)
                     /locus_tag="AC1LW8_002438"
     tRNA            complement(3201..3274)
                     /locus_tag="AC1LW8_002438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3238..3240),aa:Ile,seq:cat)
     gene            complement(3303..3376)
                     /locus_tag="AC1LW8_002439"
     tRNA            complement(3303..3376)
                     /locus_tag="AC1LW8_002439"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3340..3342),aa:Met,seq:cat)
     gene            complement(3398..3473)
                     /locus_tag="AC1LW8_002440"
     tRNA            complement(3398..3473)
                     /locus_tag="AC1LW8_002440"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3438..3440),aa:Ala,seq:tgc)
     gene            complement(3491..3564)
                     /locus_tag="AC1LW8_002441"
     tRNA            complement(3491..3564)
                     /locus_tag="AC1LW8_002441"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3528..3530),aa:Pro,seq:tgg)
     gene            complement(3574..3647)
                     /locus_tag="AC1LW8_002442"
     tRNA            complement(3574..3647)
                     /locus_tag="AC1LW8_002442"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3611..3613),aa:Arg,seq:acg)
     gene            complement(3666..3754)
                     /locus_tag="AC1LW8_002443"
     tRNA            complement(3666..3754)
                     /locus_tag="AC1LW8_002443"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3718..3720),aa:Leu,seq:taa)
     gene            complement(3765..3839)
                     /locus_tag="AC1LW8_002444"
     tRNA            complement(3765..3839)
                     /locus_tag="AC1LW8_002444"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3805..3807),aa:Gly,seq:gcc)
     gene            complement(3843..3924)
                     /locus_tag="AC1LW8_002445"
     tRNA            complement(3843..3924)
                     /locus_tag="AC1LW8_002445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3888..3890),aa:Leu,seq:tag)
     gene            complement(3928..4003)
                     /locus_tag="AC1LW8_002446"
     tRNA            complement(3928..4003)
                     /locus_tag="AC1LW8_002446"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3968..3970),aa:Lys,seq:ttt)
     gene            complement(4009..4084)
                     /locus_tag="AC1LW8_002447"
     tRNA            complement(4009..4084)
                     /locus_tag="AC1LW8_002447"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4049..4051),aa:Thr,seq:tgt)
     gene            complement(4101..4176)
                     /locus_tag="AC1LW8_002448"
     tRNA            complement(4101..4176)
                     /locus_tag="AC1LW8_002448"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4141..4143),aa:Val,seq:tac)
BASE COUNT         1359 a          931 c          791 g         1104 t
ORIGIN      
        1 aaaaatccgc tattatacgt gaacaaatct cacattttaa aatcaatcaa aaaagcgcta
       61 ctatcaatat cttattgaca gtaacgccta tatttatcat atattaaaac ttattcttgt
      121 catcaagcta taaattattg ttttggaact tcaaatttat aaatatcatt agaatatata
      181 ggaatgaatt ttttacacaa ttcatgaaca ataaatgccg atacaaatgg tatgacaaaa
      241 taaccaagtg ctaataaaat aatattcatt gttggatcac ctgacattcg gttaaatgca
      301 tttattgggc caactaatcc tgtatatcca aatccagcag acaatggtgt tcctttaacc
      361 tgaagaacgt aagcaatgat tccagtaatt attccattta tagtcaatgg tatcgaaata
      421 attaaatttt tcaagtaaac tgggatcatc atttttgcag ctcctatgag taaaactgca
      481 tttacaccaa tagaattgac acgcaatgag ccaaataaga aagtaacaca ggcagccact
      541 atacctaggt ttgctgctcc acttcctagt ccgtttaaac taatcgcagt tgcaatcgct
      601 actaacgata ttggtgttac cattaataat gaaaatgcca cactaataag tatagacatt
      661 aacaacggat ttaagtctgt aaaagaatga attacatttc caattgcttg agtaattttt
      721 cgaatgtaag gtaatgtgat tagaccgata cccccactaa cgataggtac taaaactggt
      781 aatataatta attcaaaaga tccaagtttg ttttgtaata ccatatatat aagacatgca
      841 ataataacaa ccaaactcgt atttataatg tcacctatac cttttaacat aaaactatta
      901 ttgctatata caacagcacc tgaaccaatc atagctgatg tacctactat agcagcacct
      961 gcaccattaa atttaaattg atgagcagct aaaaccccaa taataaatgc cataaatgat
     1021 tgaattagta tcactaactg atacgttaat tctaaaattt cattaccact tttaaatatt
     1081 tttaatactt cacctaataa agcattcgga acaagtgcaa taacaacacc agcaccaata
     1141 gaatttaaaa tcttactgaa aaactgttta ttatcagcat tatttgcgtt actcataaac
     1201 gacctccaat ttgaacatta aactcatcat attatagaaa attaacttta acaatgatta
     1261 gttggctaat ttaatctaaa tataacaatc cacaaattga ataaagcaaa tttaaacaat
     1321 ctactcacta atcaatgtca tgttaatttt aaaatactac taattacatc ctgtgactaa
     1381 taatgtaata aattgtaaat attaaaatag ccatattact attaagatag ataaattgaa
     1441 tcgacttggt ataaagcaca ataaaatagc acttttacaa atatattatt agaaaagttg
     1501 aaacaaatat ataaccccag ataatataat cattatcatt aatgtaatac caaaaaaaga
     1561 agttaaacaa tgtatagttg cttaacttcc aatattgaac tcatcattac aatttgacat
     1621 agagtctatt aaagcgtgtg ccatttgagt ccacttttat ttgtattgta tagagagaaa
     1681 taaaaagaaa ccttgtttta caaggtttct aatacgttat gttatgtaaa taacagttaa
     1741 ttataccggt ggtcggggtc gaaccgacac tccacaagtg gaacgggatt ttgagtcccg
     1801 cgcgtctgcc aattccgcca caccggctta atggtaaaca aaaaacttcc ctttggaagc
     1861 aattatggag cggaagatag gatttacacc tatacctcgt tccgggaagg aacgtgttct
     1921 aaaagttgaa ctactcccgc aaatattaaa ttatggagcg gaagatagga tttacaccta
     1981 tacctcattc caggaaggaa tgtattctaa gagttgaaat actcccgcat tattattaaa
     2041 ttatggagcg gaagatagga tttgcaccta tacctcgttc cgggaaggaa cgtgttctaa
     2101 aagttgaact actcccgcat aaacctggag gcggcaaccg gatttgaacc ggtgataaag
     2161 gttttgcaga cctctgcctt accacttggc tatgccgcca ataactgggc tagctggatt
     2221 cgaaccaacg agtgacggag tcaaagtccg ttgccttacc gcttggctat agcccattaa
     2281 taataagggc ggctgaaggg gatcgaaccc tcgaatgtcg gaaccacaat ccgatgtgtt
     2341 aaccacttca ccacagccgc catggcaggg gcagtaggaa tcgaacccac accaaaggtt
     2401 ttggagacct ctattctacc gttgaactat gcccctatta aaaataataa atggaggggg
     2461 gcagattcga actgccgaac ccgaaggagc ggatttacag tccgccgcgt ttagccactt
     2521 cgctacccct ccataaatgg tgccggccag aggacttgaa cccccaacct actgattaca
     2581 agtcagttgc tctaccaatt gagctaggcc ggctaagaaa tggttcagga cagagtcgaa
     2641 ctgccgacac atggagcttc aatccattgc tctaccaact gagctactga accataataa
     2701 aaatgtaatg atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     2761 gcgtgttaac cgctacacta cgagacctat aaaatattgc gggaggcgga tttgaaccac
     2821 cgaccttcgg gttatgagcc cgacgagcta ccgaactgct ccatcccgcg ataataaaaa
     2881 ataatggcgg aggaagaggg attcgaaccc ccgcggcccg ttaaggccct gtcggttttc
     2941 aagaccgatc ccttcagccg gacttgggta ttcctccatt attataggta aatcgctatt
     3001 aattataaaa ttaaatggcg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag
     3061 gcaggcgtgt taaccgctac actacgagac cattagtaaa acggaggaag agggattcga
     3121 acccccgcga gccgttaagc ccctgtcggt tttcaagacc gatcccttca gccggacttg
     3181 ggtattcctc caaaattata tggaccttgc aggactcgaa cctgcgaccg aacggttatg
     3241 agccgttagc tctaaccaac tgagctaaag gtcctaaata taattttaca actaataaat
     3301 agtggcggtg gaggggatcg aacccccgac ctcacgggta tgaaccgtac gctctagcca
     3361 gctgagctac accgccttat atagtttgta aataatatgg tggagactag cgggatcgaa
     3421 ccgctgacct cctgcgtgca aagcaggcgc tctcccagct gagctaagcc cccataataa
     3481 ttacagtata tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc
     3541 tctaccaagc tgagctactt cccgtataat taacgcgccc gataggagtc gaacccataa
     3601 cctcttgatc cgtagtcaaa cgctctatcc aattgagcta cgggcgcata tgtttttatt
     3661 gaaaatggtg ccgaggaccg gaatcgaacc ggtacggtga tcactcaccg caggatttta
     3721 agtcctgtgc gtctgccagt tccgccaccc cggcactata aaaatggagc agaagacggg
     3781 attcgaaccc gcgaccccaa ccttggcaag gttgtattct accgctgaac tacttctgca
     3841 tatgcgggtg aagggagtcg aacccccacg ccgtaaggcg ctagatccta agtctagtgc
     3901 gtctgccaat tccgccacac ccgcaaatgg tgagccatag aggattcgaa cctctgaccc
     3961 tctgattaaa agtcagatgc tctaccaact gagctaatgg ctcttccatg gtgccggcca
     4021 gaggacttga acccccaacc tactgattac aagtcagttg ctctaccaat tgagctaggc
     4081 cggcaatatg taagaataaa tggtggagaa tgacgggttc gaaccgccga ccctctgctt
     4141 gtaaggcaga tgctctccca gctgagctaa ttctccgatt taaaa
//
LOCUS       DBNENW010000178         4048 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNENW010000178 DBNENW010000000
VERSION     DBNENW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.181"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(31..432)
                     /locus_tag="AC1LW8_002449"
     CDS             complement(31..432)
                     /locus_tag="AC1LW8_002449"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIP4774211.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKIIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            776..907
                     /locus_tag="AC1LW8_002450"
     CDS             776..907
                     /locus_tag="AC1LW8_002450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774212.1"
                     /translation="MGPNTEDDEKSAYNNVQVGDGPQQREIGFPISTDNASWGGTTK"
     gene            complement(972..1676)
                     /locus_tag="AC1LW8_002451"
     CDS             complement(972..1676)
                     /locus_tag="AC1LW8_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIP4774213.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1921..2115
                     /locus_tag="AC1LW8_002452"
     CDS             1921..2115
                     /locus_tag="AC1LW8_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIP4774214.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2127..2378
                     /locus_tag="AC1LW8_002453"
     CDS             2127..2378
                     /locus_tag="AC1LW8_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HIP4774215.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            2416..3540
                     /locus_tag="AC1LW8_002454"
     CDS             2416..3540
                     /locus_tag="AC1LW8_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIP4774216.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            3556..3993
                     /gene="brxA"
                     /locus_tag="AC1LW8_002455"
     CDS             3556..3993
                     /gene="brxA"
                     /locus_tag="AC1LW8_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacilliredoxin BrxA"
                     /protein_id="HIP4774217.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT         1440 a          649 c          705 g         1254 t
ORIGIN      
        1 cgattgacat cacatcagtc ggtgctcctt ttatttattc tttttaatta atttatacaa
       61 tgcctgttga gcgtgttgat tcgcttcttt gttttgttct ctcggtatcc atttaacaaa
      121 taataaatca aaatcttttt caaatatttc tatttgatca aaataaggtt tgaaatttgc
      181 gtttttcaca taaccagctt caatgctatc tgcaattatc tttgagtctg tatataatag
      241 tgcgttttga acatttaatt cacgtgcatg ttctagtgca taaatacatg cagcccattc
      301 tgcagtgtgg ttatccattt cgcctaactc atgtgtatat gtataatgct gctcatcttc
      361 tttgattaca atggcacatg tacttatgcc tggatttcct ttcgtcgcag catcaaaatt
      421 tattttcgcc ataataaacc tactttctat tcaatactta gttaaagtta ctattactgt
      481 aatacaaaat atgttgggta atccattaaa aaacacgcat cacttaaata agtaacacgt
      541 gtttaaaata ctcgctgatt caaagatgat tttctaatac gtagtactgt aatatacttc
      601 ctaaaaaatc atcttcaggc tgggacataa atcaatgttc tatgctctac gatgttatat
      661 tggcagtagt tgactgaaca aaaatgcgct tgtaacaagc ttttttcaat tctagtcagg
      721 ggccccaaca cagagaattt cgaaaagaaa ttctacaggc aatgcgagtt ggggtgtggg
      781 ccccaacaca gaagatgacg aaaagtcagc ttacaataat gtgcaagttg gggatgggcc
      841 ccaacaaaga gaaattggat tcccaatttc tacagacaat gcaagttggg gtgggacgac
      901 gaaataaatt ttgcgaaaat atcatttctg tcccacagcc ttaaaactta ttcttttgtg
      961 tagtaagtgc gttaatagcc ttgatctaac ttatcaatct tacctttacg ataaaatgat
     1021 ttagcaatat atccaaatgg tacattgaaa actgttgaag ctaattttaa tacgtacgtt
     1081 gtaataaata tttcaaatac aactgtacca ggtaaacttc cgataaatgc gatagctaca
     1141 aataaagctg tatcaattat tgagcttaaa aatgtactac catatgcacg gatgaaaaac
     1201 gttttatcag aactaaatac ttttttaatt agtgaaaaga taaatacatc aatatgttga
     1261 ccaataatat atgcgacaat tgagcctaaa gcaatacgtg gcacaacatc aaagattgcg
     1321 tgtaatgctt tttgtgccat atcttctggt gcaggaataa aatgtaatga caattgcata
     1381 acaataatca taattaatgt tgatgaaaag cctaaccaaa ctgctctttt tgcaactcta
     1441 cgcccataaa tatcatttaa aatatctgtt gctaaataaa tagaagcaaa catgacatta
     1501 cctaaagttg ctgaaatacc aaagatttct acagttttaa tcacttgtat gttggcaatg
     1561 attgtgccaa ttgcaaccca tgcaattaaa ccttgtttac caaaaaagcg atacataagt
     1621 accataagta cgaacgttgc aataaacgta actagtccta aaatttcatt atacatatta
     1681 aaatgtcctc ctaaattttg atcatgcggg tgtttagaaa ccgctcaata aataacttac
     1741 tcattttact gaaaaatcgt ttgcgatgca atagcatttg aatataaata cattttacgt
     1801 atagaattgt aaaaggtttt attcaaatct tagggtcaaa aatgttataa tatttttatg
     1861 tcaaatttaa aacagtaaca cttatttaca aggttgcaat attttgaagt aataaaggaa
     1921 gtgtcgcgta ttttaacttt ttcagagcaa aatgcactcg cgaaaataga tgatttaatg
     1981 aatacttatt gcaatcaatg tccaatcaaa actcgtctgc gtaaattaga ggggaaaacg
     2041 aaggcgcatc atttttgtat caatgagtgt tcaataggga aagaaataaa acaattagga
     2101 aatgaacttc aataggagga agtcaaatga aaattatatc tatatcagaa acaccgaacc
     2161 acaacacaat gaagattaca cttagtgaaa gcagagaagg tatgacatca gatacgtata
     2221 ctaaagttga tgattcacag ccagcattta ttaatgacat cttaaaggtt gaaggcgtta
     2281 aatcaatttt ccatgttatg gactttattt cagtagataa agaaaatgac gcaaattggg
     2341 aaacagtatt gccaaaagta gaggctgtat tcgaataaat ttttcatcaa ctagtattcg
     2401 gggggaataa agtatatgga aattttacgt atagagccaa caccaagtcc aaatacaatg
     2461 aaagttgttt tgtcatatac aagagaagac aagttatcta atacttataa aaaagtagaa
     2521 gaaacacaac caagatttat aaatcagttg ttatctatag atggtatcac ttccattttt
     2581 catgtcatga acttcttagc tgttgataag gcaccaaaag ctgattggga agtcatatta
     2641 cctgatatta aagctgcttt ttctgatgcg aataaggttt tagaatctgt aaatgaacct
     2701 caaattgaca atcattttgg tgaaattaaa gctgaattat taacttttaa gggtataccg
     2761 tatcaaatta agctaacttc tgctgaccaa gaattaagag aacaattacc acaaacatat
     2821 gttgatcata tgactcaagc gcaaacagca catgacaata ttgtttttat gcgtaaatgg
     2881 ctagatttag gaaatcgcta tggaaatatt gaagaagtaa tggatggtgt cctagaagaa
     2941 gtgctagcta cctatccaga atcacagtta cccgtattgg taaaacatgc tttagaagaa
     3001 aatcacgcaa ctaataatta tcatttctat cgacatgtct ctttggatga atatcatgca
     3061 actgataatt ggaagactcg attacgaatg ttaaaccatt ttccaaagcc gacttttgaa
     3121 gatataccgc tacttgattt agctttatct gatgaaaaag taccggttag acgtcaagcg
     3181 attgtattat taggtatgat tgaaagtaaa gaaattttac cgtatttata taaggggctt
     3241 cgtgataaaa gtcctgctgt aagaagaaca gcaggggatt gcataagcga tttagggtat
     3301 ccagaggcac taccagaaat ggtgctacta ttagatgatc cacagaaaat cgttaggtgg
     3361 cgtgctgcta tgtttatctt tgatgaaggt aatgcagagc agcttcccgc actaaaagcc
     3421 catattaatg acaatgcgtt tgaagttaaa ttacaaattg aaatggccat atctcgtatt
     3481 gaaaacggag atgaggcact tggttcagta tggaaacaaa tggcaaacag aacaatttaa
     3541 ttggaggaat taaatatgaa tgcatatgat gcttatatga aagaaattgc gcaacaaatg
     3601 cgtggcgaat taactcaaaa tggttttaca agtttagaaa cgagcgaagc agtatcggag
     3661 tatatgaacc aagtaaatgc tgatgacact acttttgtag ttattaactc tacatgcggc
     3721 tgtgcagctg gattagcaag accagctgca gtagcagttg caacacaaaa tgaacataga
     3781 cctacaaata cagttacagt ttttgctggg caagataaag aagcaactgc tacaatgcga
     3841 gaattcattc agcaagcacc atctagtcct tcgtatgctt tattcaaagg tcaagattta
     3901 gtttatttta tgcctagaga atttatcgaa ggtagagata ttaatgacat tgcaatggac
     3961 ttaaaggatg cctttgacga aaattgtaaa tagtacacat aaataaatat aaaggttaac
     4021 acattttata atattaaaaa tggtgtct
//
LOCUS       DBNENW010000179         3959 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNENW010000179 DBNENW010000000
VERSION     DBNENW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.183"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..685
                     /locus_tag="AC1LW8_002456"
     CDS             <1..685
                     /locus_tag="AC1LW8_002456"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIP4774218.1"
                     /translation="NYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILED
                     DTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEI
                     DTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECGLEV
                     GEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESH
                     PAIKAPIAV"
     gene            885..1364
                     /locus_tag="AC1LW8_002457"
     CDS             885..1364
                     /locus_tag="AC1LW8_002457"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIP4774219.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1379..2218
                     /gene="fakB2"
                     /locus_tag="AC1LW8_002458"
     CDS             1379..2218
                     /gene="fakB2"
                     /locus_tag="AC1LW8_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HIP4774220.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2304..2837
                     /gene="msrA"
                     /locus_tag="AC1LW8_002459"
     CDS             2304..2837
                     /gene="msrA"
                     /locus_tag="AC1LW8_002459"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIP4774221.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2830..3258
                     /gene="msrB"
                     /locus_tag="AC1LW8_002460"
     CDS             2830..3258
                     /gene="msrB"
                     /locus_tag="AC1LW8_002460"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIP4774222.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3270..3770
                     /locus_tag="AC1LW8_002461"
     CDS             3270..3770
                     /locus_tag="AC1LW8_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP4774223.1"
                     /translation="MFKKLFGKGKEVQKDIAIYTPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3770..>3959
                     /locus_tag="AC1LW8_002462"
     CDS             3770..>3959
                     /locus_tag="AC1LW8_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIP4774224.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDL"
BASE COUNT         1432 a          589 c          723 g         1215 t
ORIGIN      
        1 aaattatata aaatcagacg agtataacgg tccagatatg acagatttcg ggcatcgtgc
       61 attgagtgat cctgaattta acgaacaata taaagaacaa atgaaacaat ttaagcaacg
      121 tattcttgaa gatgatactt ttgcgaagca attcggtgat ttaggaaatg tttatggtaa
      181 acaatggcga gattgggttg ataaagatgg taatcatttt gatcaactta aaacagtaat
      241 tgaacaaatt aagcataatc cagattcaag acgacacatc gtatctgcat ggaatccaac
      301 agaaattgat acaatggcac ttccgccttg tcataccatg ttccagtttt atgtccaaga
      361 tggtaagtta agttgccagt tataccaacg tagcgcagat atctttttag gtgtgccatt
      421 taatatcgca agctacgctt tattgacaca ccttattgcc aaagaatgtg gacttgaagt
      481 aggagaattt gtgcatacat ttggagatgc acatatttat tcaaatcata ttgatgcgat
      541 tcaaacacaa ttagcacgtg aaagcttcaa tcctccaaca ttaaaaatta acagtgacaa
      601 gtctattttc gacataaatt atgaagattt ggaaattgtt gactatgaat cacatccagc
      661 aataaaagct ccaatagcag tgtagtcatt gcatagttag ctaaccatat agacatcaaa
      721 atgacatcat agtattttca agtgcaaaaa agtacttttt tgtgttaaac gttttcataa
      781 attatgcaaa atcattattt ctatcacact ttatgataaa aattgtgtta aattaaagat
      841 aacttagtaa taaaaaatga aatgatagaa gaaggaggat aattatgact ttatccattc
      901 tagttgcaca tgacttgcaa cgagtaattg gttttgaaaa tcaattacct tggcacctac
      961 caaatgattt gaaacatgtt aaaaaattat caacaggtca tactttagta atgggtcgta
     1021 agacatttga atcgattggt aaaccactac cgaatcgtcg aaatgttgta cttacttcag
     1081 atacaagttt caacgtagtg ggcgttgatg taattcactc tattgaagat atttaccaac
     1141 taccgggcca tgttttcata tttggagggc aaatattatt tgaagaaatg attgataaag
     1201 tggacgacat gtatattact gttattgaag gtaaattccg tggtgatacg ttctttccac
     1261 cttatacatt tgaagactgg gaagttgcct cttcagttga aggtaaacta gatgagaaaa
     1321 atacaattcc acataccttt ctacatttaa ttcgtaaaaa ataaggggga aaacgaccat
     1381 gacaaaacag attatagtaa cagactcaac atccgattta tctaaagaat acttagaagc
     1441 aaacaacatt catgtaattc ctttaagttt aactattgaa ggagcttcat acgttgacca
     1501 agtagatatt acatcagaag aatttattaa tcatattgaa aatgatgaag atgtaaagac
     1561 aagtcagcca gccataggtg aatttatatc tgcttatgaa gaactaggaa aagatggctc
     1621 tgaaatcata agtattcatc tttcttcagg attaagtggt acatataaca ctgcttacca
     1681 agcaagtcaa atggtagatg ctaatgtaac tgttattgat tcaaaatcta tttcttttgg
     1741 tttagggtat caaatacaac acctagtaga gcttgtaaaa gaaggtgtct caacttctga
     1801 aatagttaaa aagttaaatc atttaagaga aaacattaaa ttatttgtag ttatagggca
     1861 attgaatcaa ttaattaaag gtggcagaat tagtaaaaca aaaggtttga ttggtaatct
     1921 tatgaaaatt aaaccaattg gtacactaga tgatggtcgc ttagagcttg tgcacaatgc
     1981 gagaactcaa aattctagta tccaatactt gaaaaaggaa attgctgaat ttataggaga
     2041 tcatgaaatc aaatccattg gtgtcgcaca tgctaacgtc attgaatatg ttgataaatt
     2101 gaagaaagtt tttaatgaag cttttcatgt gaataattac gatataaatg taactacacc
     2161 agttatttct gcacatactg gtcaaggtgc gattggcctc gtagtcctta agaagtaaat
     2221 ttaatctttt cagtgttaat tacttccatt tcaatccttt atagactaaa tttataatta
     2281 gatagataga ggaggtaatt catatgacaa aagaatatgc aacattagca ggaggatgtt
     2341 tctggtgcat ggttaaacca tttacatcat atccaggcat caagtcagtc gtatctggtt
     2401 atagtggcgg tcatgttgac aacccaactt atgaacaggt atgtacgaat caaaccggcc
     2461 atgtcgaagc agtacaaatt acgtttgatc cagaggttac ttcctttgaa aatatattag
     2521 acatatattt caaaacattt gacccaactg atgatcaagg gcaatttttc gatagaggcg
     2581 aaagctatca accagtcatt ttctatcatg atgaacatca gaaaaaggct gctgagttta
     2641 aaaagcaaca attaaatgaa caaggtattt tcaagaaacc agtgattaca cctattaaac
     2701 catataaaaa tttctatcca gctgaagact accatcaaga ttattacaaa aagaacccgg
     2761 tacattatta ccaatatcaa cgtggttcag gtagaaaagc gtttatagaa tcacattggg
     2821 ggaatcaaaa tgcttaaaaa agataaaagt gaactaacag atatagaata tattgttaca
     2881 caagaaaacg gcactgaacc accatttatg aatgaatatt ggaatcattt tgctaaagga
     2941 atttatgtag ataaaatttc tggtaaacct ttatttacat ctgaagaaaa gtttcattct
     3001 gaatgtggat ggcctagctt ttccaaagcg cttgatgacg atgaaattat agaattagtc
     3061 gacaaatcat ttggtatgtt gagaactgaa gtgcgttcag aagaatcaaa tagtcattta
     3121 ggacatgtct ttaatgatgg acctaaagaa agtggcggct taagatactg tatcaattcc
     3181 gctgcaattc aatttattcc atatgaaaag ttagaagaat tgggttatgg cgatttaata
     3241 tcacattttg ataagtagga gtggaaagta tgtttaaaaa attattcgga aaaggcaaag
     3301 aagttcagaa agatatcgca atttatacac cattaactgg agaattcgtg aaaattgaag
     3361 atattccaga tcctgtattc gcacaaaaaa tgatgggcga aggttttggt attaatccaa
     3421 ctgaaggaga agttgtgtct ccaattgcag gacgtgttga caatgtcttt ccaactaagc
     3481 atgcgattgg gttaaaagca gataacggat tagaattatt agttcatatc ggtttagaca
     3541 cagttcaatt agacggtgaa ggatttgaag tgttagtgtc tagtggtgac gaagttaacg
     3601 tcggagatcc attagtaaga ttcaaccttg aatatattaa taataacgct aaatctgtaa
     3661 tttcaccaat tataattact aacactgatc aagcagcttc aattaatatt tatgatgaaa
     3721 atgctgtgat taaaggtgaa acaaaagtga ttgatgtgac aatgaactaa tgaaaaacta
     3781 ttcgttttat caatttgtca tgacagttcg tggtcgacac gacgataaag gtcgtctagc
     3841 agaagagata tttgacgatc ttgctttccc aaaacacgat gatgatttta acatactgtc
     3901 tgattatatt gagacacatg gtgatttcac attgccaatg tctgtatttg atgatttat
//
LOCUS       DBNENW010000180         3882 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.184, whole genome shotgun sequence.
ACCESSION   DBNENW010000180 DBNENW010000000
VERSION     DBNENW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.184"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            55..1581
                     /gene="vwb"
                     /locus_tag="AC1LW8_002463"
     CDS             55..1581
                     /gene="vwb"
                     /locus_tag="AC1LW8_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HIP4774225.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            1932..2954
                     /gene="emp"
                     /locus_tag="AC1LW8_002464"
     CDS             1932..2954
                     /gene="emp"
                     /locus_tag="AC1LW8_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HIP4774226.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPTYNEFKKAPKVNVGNLADNKNFVASEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            3289..3810
                     /locus_tag="AC1LW8_002465"
     CDS             3289..3810
                     /locus_tag="AC1LW8_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774227.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVSESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSVVEKSQLNTLKSLSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKYTQKYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
BASE COUNT         1636 a          529 c          608 g         1109 t
ORIGIN      
        1 aaaatagggt gtaaggtagg ttgttaatta gggaaaatta aggagaaaat acagttgaaa
       61 aataaattgc tagttttatc attgggagca ttatgtgtat cacaaatttg ggaaagtaat
      121 cgtgcgagtg cagtggtttc tggggagaag aatccatatg tatctgagtc gttgaaactg
      181 actaataata aaaataaatc tagaacagta gaagagtata agaaaagctt ggatgattta
      241 atatggtcct ttccaaactt agataatgaa agatttgata atcctgaata taaagaagct
      301 atgaaaaaat atcaacagag atttatggct gaagatgagg ctttgaagaa attttttagt
      361 gaagagaaaa aaataaaaaa tggaaatact gataatttag attatctagg attatctcat
      421 gaaagatatg aaagtgtatt taatactttg aaaaaacaaa gtgaggagtt cttaaaagaa
      481 attgaagata taaaaaaaga taaccctgaa ttgaaagact ttaatgaaga ggagcaatta
      541 aagtgcgact tagaattaaa caaattagaa aatcagatat taatgttagg taaaacattt
      601 tatcaaaact atagagatga tgttgaaagt ttatatagta agttagattt aattatggga
      661 tataaagatg aagaaagagc aaataaaaaa gcagttaaca aaaggatgtt agaaaataaa
      721 aaagaagact tagaaaccat aattgatgaa ttttttagtg atatagataa aacaagacct
      781 aataatattc ctgttttaga agatgaaaaa caagaagaga aaaatcataa aaatatggct
      841 caattaaaat ctgacactga agcagcaaaa agtgatgaat caaaaagaag caagagaagt
      901 aaaagaagtt taaatactca aaatcacaaa cctgcatctc aagaagtttc tgaacaacaa
      961 aaagctgaat atgataaaag agcagaagaa agaaaagcga gatttttgga taatcaaaaa
     1021 attaagaaaa cacctgtagt gtcattagaa tatgattttg agcataaaca acgtattgac
     1081 aacgaaaacg acaagaaact tgtggtttct gcaccaacaa agaaaccaac atcaccgact
     1141 acatatactg aaacaacgac acaggtacca atgcctacag ttgagcgtca aactcagcaa
     1201 caaattattt ataatgcacc aaaacaattg gctggattaa atggtgaaag tcatgatttc
     1261 acaacaacgc atcaatcacc aacaacttca aatcacacgc ataataatgt tgttgaattt
     1321 gaagaaacgt ctgctttacc tggtagaaaa tcaggatcac tggttggtat aagtcaaatt
     1381 gattcttctc atctaactga acgtgagaag cgtgtaatta agcgtgaaca cgttagagaa
     1441 gctcaaaagt tagttgataa ttataaagat acacatagtt ataaagaccg attaaatgca
     1501 caacaaaaag taaatacttt aagtgaaggt catcaaaaac gttttaataa acaaatcaat
     1561 aaagtatata atggcaaata attaatgcat ggctgcaaag caaataatga gtttgtcgta
     1621 aaaataacaa cattttaaac tagcaataaa taatatcaaa gtcatcattt caatgatgca
     1681 atctagtata gtccacattc taaacaggtg tggactatta cttttttcac tttatattac
     1741 gaaaaaatta ttatgcttaa ctatcaatat caataattaa ttttaagctg aaaaacaata
     1801 aaaatgttaa gacaacgttt acttcaagtt aattattata ctgaaaattc tggtatataa
     1861 tgctgttagt gaatataaca gggaaattat attggttata atattgagtc tatataaagg
     1921 agaaataaca gatgaaaaag aaattattag ttttaactat gagcacgcta tttgctacac
     1981 aaattatgaa ttcaaatcac gctaaagcat cagtgacaga gagtgttgac aaaaaatttg
     2041 tagttccaga atcaggaatt aataaaatta ttccaactta caatgaattt aaaaaagcac
     2101 caaaagtaaa tgttggtaat ttagctgaca ataaaaactt tgtagcttct gaagataaat
     2161 tgaataagat tgtagattca tcggcagcta gtaaaattgt agataaaaac tttgccgtac
     2221 cagagtcaaa gttaggaaac attgtaccag agtacaaaga aatcaataat cgcgtgaatg
     2281 tagcaacaaa caatccagct tcacaacaag ttgataagca ttttgttgct aaaggcccag
     2341 aagtaaatag atttattacg caaaacaaag taaaccacca cttcattact acgcaaaccc
     2401 actacaagaa agttattact tcatacaaat caacacatgt acataaacat gtaaatcatg
     2461 caaaggattc tattaataaa cactttattg ttaaaccatc agaatcgcct agatatacac
     2521 atccatctca atctttaatt atcaagcatc attttgcagt tcctggatat cacgcgcata
     2581 aatttgttac accagggcat gctagcatta aaattaatca cttttgtgtt gtgccacaaa
     2641 taaatagttt caaggtaatt ccaccatatg gtcacaattc acatcgtatg catgtaccaa
     2701 gtttccaaaa taacacaaca gcaacacatc aaaatgctaa agtaaataaa gcatatgact
     2761 ataaatactt ctattcttat aaagtagtta aaggtgtgaa gaaatatttc tcattttcac
     2821 aatcaaatgg ttataaaatt gggaaaccat cattaaatat caaaaatgta aattatcaat
     2881 atgctgttcc aagttatagc cctacacact acgttcctga atttaagggt agcttaccag
     2941 caccacgagt ataaaaattg gcactaagtt tacgagatat gataaatacc tattatttta
     3001 aatatagtct acaatctatg tggttgtagg ctgtattttt tgcagtttat caataaacac
     3061 ccatcaacaa attataccgt ttttctactt tgaaagttgg aagtaacata atcttaaata
     3121 aatatattat taattaagat aaatataata ctcaagatta ttgttaatag tttgttcatc
     3181 gcaagttaat tattgtttct aaaatattgg tatataattt tcaatggcga agaaaacagg
     3241 gtgaaaaagt cggtttttaa atcaaagcaa ataagggagc ataaacaaat gaaaaggaaa
     3301 gtattagtac taacaatggg tgtaatttgt gcaactcaat tatggcattc taatcacgca
     3361 aacgcattag tatcagagag tgttgaaact aattttgttg taaaagattc ggataataaa
     3421 aatattttac aaacccatac tgaaattact actgaggaga aattttcagt agtagaaaag
     3481 agtcaattaa atacactaaa atcactatct aatgacaact acatagaata tgatttacat
     3541 actaatcaaa caggcataaa aaaaggttgg ttatatggtt atagtgaaat tgactcatca
     3601 catttcacag accgtgacaa acgcgttatt agacgtgatc atgttaaaga agcacaaagc
     3661 ttaattaatg attataaata tacgcaaaaa tatgaagatc tcgctaaggc aactgcaaaa
     3721 gtaagtacac ttagtcagtc tcaccaaaat tatttgaata aacaaattga taaagtgaat
     3781 aataagatag agaaaactga aaaacgctaa tccaaagtaa attataagtt atacatctcg
     3841 tttttaaatg acaatttatc cccgtaaata ttataaataa tc
//
LOCUS       DBNENW010000181         3859 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.185, whole genome shotgun sequence.
ACCESSION   DBNENW010000181 DBNENW010000000
VERSION     DBNENW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.185"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..1503)
                     /locus_tag="AC1LW8_002466"
     CDS             complement(<1..1503)
                     /locus_tag="AC1LW8_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4774228.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWI"
     gene            complement(1518..1832)
                     /locus_tag="AC1LW8_002467"
     CDS             complement(1518..1832)
                     /locus_tag="AC1LW8_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HIP4774229.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(1810..2628)
                     /gene="recX"
                     /locus_tag="AC1LW8_002468"
     CDS             complement(1810..2628)
                     /gene="recX"
                     /locus_tag="AC1LW8_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIP4774230.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(2889..3698)
                     /gene="sgtB"
                     /locus_tag="AC1LW8_002469"
     CDS             complement(2889..3698)
                     /gene="sgtB"
                     /locus_tag="AC1LW8_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HIP4774231.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
BASE COUNT         1169 a          665 c          488 g         1537 t
ORIGIN      
        1 aatccatttg tcttctttca aattggcaat acaatatcca ttcccaactt tagtaatcca
       61 aatgttattg ttaatattaa acttatcttt taattctttt ttatctacaa ttggataaac
      121 aaaaccgtat aactgattat tatcattgaa aagtaattgt atcggttgtt gtgtaacgga
      181 tgcaacgaaa cggttatcat catcaggaac taaagttttt ataactgaac tatgcttttt
      241 atgtaattga ctattaaagt atgcatagta attactataa tttgatttca tatatggtgc
      301 taatttttta aaactatgcg tttgatataa acctgccggg ttaaaatatt ctaatttatc
      361 tttactaaca tagtaagtct tattatcggc ggtaacacga taatttttac tattttcgcc
      421 agtaattgta actacactat attttggaat tgtgacttgt ttatcccctt gcatatccac
      481 actgccatta aaagcaattc cataagctaa cttttcgcca tatggatctt tattttcatt
      541 ttgcatcgta gcacgattcg taaaatctgt tatcgaaata attcctatat tattgaaaat
      601 gagcaacatc atcaatgcta agcctaaaat agtacctgag gctaaagtcc aaaaacgcac
      661 taaaaactta ggcggttttg catgattata gcgctcaaca cgtttgaaat tataggtcat
      721 ctctggtatt cttgtacgat tctttttcca atctaaatca aagttttcaa tttcttcttt
      781 tgagtttaga cttaatcgat cacgttcatg ttctttaaaa gatggaataa cttgtttaag
      841 tgacccttcc attcttaatt gaccatgtga aaaccaagct atgtagttac tcacttgtga
      901 aattttatca atatcatcac caattgacac aatcgttaaa ttattttcaa tataatcatt
      961 tgttaattca atcgcacgtt ccataaattg tggtgttaaa tagtcaataa catgatttaa
     1021 aataataata tttgattttg atgagcgtgc aatacttaat agtaattgag cgtatgccgc
     1081 tttcgaaata tggttaaccg gcttcgtttt ataatcacct aaatgtgcat attgaataat
     1141 ctgttcagct ttatgatcat taatttcata tggaaatagt tgaactaact gcgctgtata
     1201 agcttcaaca gtttgatgaa ttaacgattg atcttcaata tatccgtaga acaaatcttc
     1261 agtacaaact actttacctt tatcaggttt aattgcacct gccaacaatt gacctaccaa
     1321 cgctttggaa gattcaggtt caccaattat acctaatgct tctccttgat aaatatgtaa
     1381 actaatattg tttaaatcga tatcttcagc atcatatcca aaaggtaaat accatttctt
     1441 attctgttta ttcctatagt agtgtgttac ttttagtaac tttaaaacaa ttgaacttcc
     1501 catctatttt catccttcta taattgtaat aacgctatag gtaatccttc ttcgggttca
     1561 tcgctattaa atctaaaccc ccacgcaaaa atacctttta atctttctac tttaaaatat
     1621 tgattactgc catcagtcag tttataaatt ttaccaattt ctatcttttt tctgtctact
     1681 aagtaacttt cggcaacaat tactttactt tgataaacgt catattcatt taaaatacca
     1741 ttcatctcag cttttctcat tttctcttta taaagttgta tttcgtgtct taattctggc
     1801 tcagacatct cactcaattt cttctgttcc atcggcaata ccactttctt ctaatttagc
     1861 tttaatttta tcatatttat atccttttct cataaggcct tcgatagttt ttgaaattaa
     1921 tttctgttgc gtgtacttct ttcgattttt attataaatt ttttctaaat ctcgttgtaa
     1981 taaatcgtct aaaaccgctt catcttgtgt aaaatccatt tcattcaata cagcatgaat
     2041 ggtttccatt tcaaaccctt tttgaattaa agattgcatt actttttctt taactttatt
     2101 ttgtggccct ttttttgttt tagatatttt ttcagcaatt tggataatat catcaagttc
     2161 ctgttgttct ctataaagtt ctgtaaatat ttcaatgata tttggttcta taccaagttg
     2221 atatagtttt tgttgataaa ttttaggtcc tttatctgtc gtgcgaatca ttgtattttt
     2281 taaactttcc gcataatctt gatggtcgat taacttttcg cgataacaat attcaatcac
     2341 ttcagaaatc gcttgctctg atatctcttc tttttgtaaa tattgtataa cttctttttc
     2401 agttctcttt ttatatgata aatattggat tgctttgttt aaacctatgc gataatgatc
     2461 atacttttga atctctgcca tgtcagcagc ttcaagttgt tgcccttttt ttaaattaaa
     2521 tttgactaat gtatcgatat ctatacccat ttcaaattgt tcgtctaaaa aaagattaaa
     2581 acgttctttg tttttctttt gaacttctat tttagtaatc ttcggcactg gatcaccccg
     2641 ttactttctc aagttttaat acaatactct tttattattc aataagccac ttcctatagc
     2701 aaatgtttaa ctttaaatat ttttcgatgc taacaaaaaa tcacactatc atcttttaaa
     2761 atgaaagtgt gattacaagc aaatctgtaa aatttataaa gcagaaacaa ttcaacttta
     2821 tcattatgac atttcaatta aaccttctac attatagttc caagcatctt acacatgaat
     2881 gcaagtattt aacgatttaa ttgtgacata gcctgttgat attgtgtttc attgatataa
     2941 ttttgttgct tcattttttc taagttcgtg cttacacgtt gcgtaaaatt ctctgacata
     3001 ttattgatat tatatacgct aggtgcattg actttactag ctaaaatagc gctttgtaaa
     3061 actgttatgt gagacattgt tgtactattt ttattcacgg ttgttccaaa gtaatggttt
     3121 gctgcgccct caagcgtata ttgattatcc ccaaagtaaa tattatttaa ataaaagctt
     3181 aaaatttcgt tcttattata ttgtttttca actcgatgag ctacaaataa ttcttttact
     3241 tttctagtaa atgaacgatc attatcataa aaataatttt tgacaacttg ttgtgtaatg
     3301 gtactaccac cttgcacatc tctgtcgcta atcgttgaaa ataaagctct agttgtacct
     3361 ttcaaatcga atccatgatg attgtagaat cgttcatctt ccattgaaat aaaggcacct
     3421 ttaacatact ctggcatgtt atcagctgac acaaaactac ttttattttc aatttttctt
     3481 agttcatcca cattatcgcg tgtagataaa aaatacatga taccaataaa caatgcgata
     3541 atgattagaa tggttaataa tatttttaat agtattcgtt tacttttttt ctttttcggc
     3601 ggtttgccaa ctggttgata atacgtatta tagtgaggtt cgtgtttcat atgctcaaaa
     3661 tgttcatttg agtttgagta cctatcgctt cttttcatgc gtttgctcct tcttttaaaa
     3721 ctcacttagt atataccttg agtttaccag tactatcaca aataggctac actttttggg
     3781 aaaatcagtc caagggctta caatcgtata cgccatcata cttacttttt gttttttgaa
     3841 aagattatag ataaatcat
//
LOCUS       DBNENW010000182         3832 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNENW010000182 DBNENW010000000
VERSION     DBNENW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.186"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..528
                     /locus_tag="AC1LW8_002470"
     CDS             <1..528
                     /locus_tag="AC1LW8_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIP4774232.1"
                     /translation="NDNVKHNIKKYNIIPAWVLFQNFSFGDLSTFYRTTLPTYRNKVS
                     KRIENLIEKNTGISIKLPEKLLCAWLNSIRFLRNRIAHTDIIYGINFTNTCAKHHSDE
                     EMYVNIEKYKYQQRLVTFLLAMKKIFMSMPENNIIEWNETLTKIENKCSEHNFIKLSR
                     LGVIENNLSYFKITK"
     gene            complement(1154..1546)
                     /locus_tag="AC1LW8_002471"
     CDS             complement(1154..1546)
                     /locus_tag="AC1LW8_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774233.1"
                     /translation="MLEDNGDIRLLYQSIEELASVLGQSQFETKTVSLIFVQMDLRYS
                     VFEKVQTDFMKYLAHKNSDEIKYIDLIKIIENALPEDKELSINVINSIIIGFANNYYP
                     ILQSLVPEIQSSYGITINKNFNIDSIDF"
     gene            complement(1568..2047)
                     /locus_tag="AC1LW8_002472"
     CDS             complement(1568..2047)
                     /locus_tag="AC1LW8_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774234.1"
                     /translation="MKITKAIGSLVLTAVLLTSVSPLTNANEIQTNNTKTAHQSYENI
                     NGVKINRNPNLPMNPEAQQRIGGTKVVKTAIKLIINNRGKAKNVIQQVGGKKAATIFD
                     KGYSGIVKELKPLLKWSEVPANAVGDAVKRGAINAGLSKSDAGIISTVVKEAIAWLF"
     gene            2970..3305
                     /locus_tag="AC1LW8_002473"
                     /pseudo
     CDS             2970..3305
                     /locus_tag="AC1LW8_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1329 a          495 c          653 g         1355 t
ORIGIN      
        1 aacgataacg ttaaacataa tattaaaaaa tacaatatta taccagcttg ggttttattc
       61 caaaatttta gttttggaga tttgtctact ttttatagaa ctacattacc aacttataga
      121 aataaagtaa gtaagcgaat tgaaaattta atagaaaaaa atactggtat aagcataaaa
      181 cttccagaaa agttattgtg tgcatggttg aatagtatca ggtttttgag aaatagaata
      241 gctcacacag atataattta tggaattaat tttacaaaca cctgtgcaaa acatcatagt
      301 gatgaggaaa tgtatgtaaa tatagaaaag tataaatatc agcaaaggtt agttacattt
      361 ttattagcta tgaagaaaat atttatgagt atgccagaaa ataatattat tgaatggaat
      421 gagactctaa ctaagataga gaataaatgt tcagaacata actttattaa actttctagg
      481 ttaggagtta ttgaaaataa cctttcttac tttaaaataa ctaaataaaa aatagcgcac
      541 actaataact aagtgattgt ctttttatgc tgaataatgc cctgtgttgt agttagaaag
      601 attttcaaat aacactatat ttataaaaat gttgaattat aatatggtcg gtttatttta
      661 taggagtaat aaaaagcatt tatcttttat ccgccacaaa taatgagtaa atgtaccttg
      721 ctttcaatgt tgatatttta caaccttgta gagttgattt gatatatcat ctcatacgtt
      781 gtaggcaatg aatacgtaaa ttatgcagtg acatgaactg ttgagaagta tagagcattg
      841 aaaatcttga tatatcaata gttataaact ggtacaaatt aacgaaaagt actcttgtca
      901 tcgagtggga ataattatat atagcaaatg ataggctaga agtaccgtta ttacgcggtt
      961 tctagccttt tttggtgcga ttggtatggg caacatcatg tgatgaaatg atgggagtta
     1021 tatctgtaga aattggcgta ccaatttctt tatgttgggg ctccgcagtc aactactacc
     1081 aatataagat tgtagagcct acgatttgga ttttatgtcc tgggatcggt ttcctattct
     1141 tattaaatag tatttaaaaa tctatactat ctatattgaa atttttattt atggttatgc
     1201 cataactact ttgtatttcc ggtactaaac tttgcaatat tgggtaatag ttattagcga
     1261 aaccaataat aatggaatta atcacattga tactaagttc tttatcttca ggtaatgcat
     1321 tttctattat tttgattaag tctatatatt ttatttcatc agaattttta tgtgctaaat
     1381 acttcataaa atcagtttgc actttttcga aaacactata tcttaagtcc atttgtacaa
     1441 atattaagct gacagtttta gtttcgaatt gactttgacc caaaactgaa gcaagttctt
     1501 ctattgattg atatagcaat ctgatatcgc cgttatcttc aagcatatga aacactcctt
     1561 taacatttta gaataaccat gcaatggctt ctttgactac agttgagatt attccagcat
     1621 cacttttact tagaccggca tttattgcgc ctcgtttaac tgcatcaccg acagcgttag
     1681 ctggtacttc tgaccatttt agcaatggtt tcaattcttt tacaatacct gaataccctt
     1741 tatcaaaaat agttgcagct tttttgccac caacttgttg tatgacattt tttgctttgc
     1801 ccctattatt aataattagc tttatagcag ttttaactac ttttgttccg ccaattcgtt
     1861 gttgtgcttc tggattcatt ggcaagttag gattgcgatt aattttcaca ccatttatat
     1921 tttcataact ttggtgagca gtttttgtat tgtttgtctg tatttcattt gcgttagtta
     1981 aaggtgaaac agacgttaat aaaacggcag tcaagactag tgaaccaata gcttttgtga
     2041 ttttcattat acatttatct cctttaatag tgaatttgca aaaccaaatg tttgaaatat
     2101 aagtaaataa tcattaaaaa aacaaagtga gtgaaaggtc ctaattcgta taactagcat
     2161 ttaataattt aattaatcgt tttcaccaaa atagaaaatg ccacttaaca caattattaa
     2221 aagtaatgta actatgaaag aagtccaagc taatacaagt gaacatatta aaagtactaa
     2281 tgaagtgact gtttgtaaga acccaaaaag taaagattcc attatataag caaaataaat
     2341 agttagtgaa aaaattgcta cagcaagtaa catttgccct atgattttca aaattccagt
     2401 tgtgtagcca gtaactaata aatttttcac atgaacttga ttaccatatt gccatcccgt
     2461 tcataaaata gtttagaaga gattgctatt aaatttaatt gttttgcata agtgtcagaa
     2521 tattattctt aactgttaag agtcgttaat ttatttaaat ctattaagat tgttagagat
     2581 tattatgcgc tgggaaacgc cactgttaca gtggaagagg aatttggata actagatatg
     2641 ttaatcgtaa atgtggcgtc gtgaaacacg acttcaacat cttcgatcaa tctatctttg
     2701 agattggtcg aagattgaaa catatgtaag aaaacgacat agtttatgga aagctcggta
     2761 agtagttgaa aaatcaatct agattgaaat catgcatatc gtttaataaa atttttcgga
     2821 gaaataaaaa cttactaaaa acgaaaaggt ttatttgatt tatttacttg tatattgtaa
     2881 atggttaaaa tgagtctata gattatgtag agaactctag tatgcctttt gagtatatgt
     2941 gggattcttt ctttatataa aaggagttcg gagtggcaac actaaaagat gtaaagactg
     3001 gtaaaaagga aattttgcca tcaactgcaa aagataaaaa tggtaaaaat gttacgttaa
     3061 tttattttga aaaagacggt aaattaggtt tttatgtgca gaaaacacaa aaagaacgtg
     3121 gagtagggaa atgcgtttct ggtatagcgg gtggcgcagt gacaggaggc actactttag
     3181 gtcttgcagg tgcaggagta ggaacagtta ctattccagt aattgggaca gttagtggag
     3241 gcgtagttgg agctgttggt ggtgctgtcg gcggtggtct aaccggtgga gccacattct
     3301 gctaatagtg taggagtgat accatgaaaa aattttttaa tctatgggta tgttcgttct
     3361 tatttttagt aataatatca tgggtaataa ttagtataaa atttggtaat atcagcacga
     3421 tgcaaatatt aatttctaat ctgtttatat ttattgctta tttactatat ttagttaagt
     3481 tcattcgttt caaaaaaaca gtggcgatca gtaaacaagt ataaagaaag tcgattttgt
     3541 ctttctctcg aacatatata agtttatata gatgtagagc tattttttat ttgtaaatta
     3601 ttttgtaatt gaaaatgagt atgaacatgt tctccaatca acagagtatg tctcggaaga
     3661 aatctattat acttatgtga ataagtcata atgtcagaca tatatataat aagacgaata
     3721 gattgtaaat agctttttac ttttaattgt tgatattcca atattagtac ctatatttat
     3781 tgaagatata gtgagatttg aggatagatg tattttaaat tatagaattg at
//
LOCUS       DBNENW010000183         3814 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNENW010000183 DBNENW010000000
VERSION     DBNENW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.187"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            64..1104
                     /locus_tag="AC1LW8_002474"
     CDS             64..1104
                     /locus_tag="AC1LW8_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIP4774235.1"
                     /translation="MQQVISDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1161..2003)
                     /locus_tag="AC1LW8_002475"
     CDS             complement(1161..2003)
                     /locus_tag="AC1LW8_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP4774236.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2000..2557)
                     /locus_tag="AC1LW8_002476"
     CDS             complement(2000..2557)
                     /locus_tag="AC1LW8_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAS signaling domain-containing protein"
                     /protein_id="HIP4774237.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(2617..3141)
                     /locus_tag="AC1LW8_002477"
     CDS             complement(2617..3141)
                     /locus_tag="AC1LW8_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774238.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            3262..>3814
                     /locus_tag="AC1LW8_002478"
     CDS             3262..>3814
                     /locus_tag="AC1LW8_002478"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP4774239.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKL"
BASE COUNT         1359 a          650 c          514 g         1291 t
ORIGIN      
        1 aatatacata cgattactat ttatttacaa tatattacaa ctaaaaagaa aggggccgat
       61 gatatgcaac aagtgatatc agatattatg acttttagag gttcacattt cgagttaggt
      121 gttaaaactg gtaaatggct tcaacaaaca cctcttctaa aaaatcgaga aaaagaatgg
      181 aaaaaacgtg tcccacgttt cgatatagat gtaaatgaaa cttatcaaat attccaaact
      241 tacgcacctc aaatatggga agaattaatg ggactacaaa gtatactcaa aatgccaaca
      301 cgccaaatta ttttaaactt tggacattat cgttttaccg atttaaaaga aagtggttgc
      361 acggtctttc aaggcaaaga cttcatggtt agaaattatg attaccaccc tgcaacttat
      421 gatggtcgct acttactata tcaacctaca gatagcggtt tagctcaaat tggtcctgta
      481 tcaagggtaa ctggaagaat ggatggtatg aatgagtcag gattaactat gggctataac
      541 tttatgcacc ggaaaaaacc agcaaatggt tttgtatgtt atatgattgg tcgtctaatt
      601 cttgaaaatt gccgaaatgt aacagaagca atacaattat tgaaagaaat accacacaga
      661 agctcattta gctacatact gatggacaaa tcattgaacc atgcaattgt tgaagtgaca
      721 ccgagatcaa tagatgttcg ttatgataat atatgtacaa atcattttga aatattaaca
      781 catgaaaatc gtaattatac aaaagaatct aaagaaagac tggcgcgtac catttcacaa
      841 actaatgata atttagatat gactacagca tttaagttat tcaataatcc tcagtatgaa
      901 atttacagta aactattcaa aagctggtct ggcaccatcc atacatcaat gtatcaacct
      961 gaaacattaa ctgcatattt tacattggga gaaaatgcgc caccagaaat aatagatttc
     1021 aaatcatggt tagatggtca agaccttaat atcactcact ttactggcaa tatagatacc
     1081 gacttaactt ttgccaacaa ataaataaca ccgatattag agaaattatg cttcctaata
     1141 tcggtgctta catcctttat ttattcaact ttgatattac catcaatcgt ataaaattct
     1201 aaaacattgt cgcttttccc aactttacca ttagtaaatg ttttattttt aactacgcta
     1261 tcacccgttc ccggatttag ctttagtata gtgtcttcag gtttactatc atacttaaaa
     1321 cgaatatctc cttgtttagt agaagctttt gcatctactt ttgatggcat gtttttaaat
     1381 tcaatgtcac catgatcgtt taaaaatatg gattgtttaa tttgactctt gtcgatttgt
     1441 agcgtaccaa taacaacttt aatattgcta tttttaatgt tacttttact aatataagta
     1501 gaactattat ttgtctttga atctagtgcg tcaagatttg agtttttaat aactacttca
     1561 ccgttaatgc tttgaatact tgtgttctct aaatcaacat cactgattct aacacttcct
     1621 gctccagatg atagatttaa acgtttaatc attttatcag gcatttcaat cgttaacgtt
     1681 ttcttattct catgaaaagg attcatatca attgcatatc ctcttgtctt agacctttta
     1741 tcactaatct tcaacgtctt atctactata ttaattaata tatcattgtc accatcatat
     1801 ttaactctaa attgattccc ccgttttata cgaagttctg tactatccaa atttatagat
     1861 acattgtcaa aagcatcgtc tttgaatgtt ttatcatatt gtttagtacc atatttgttt
     1921 ttatcatagc tgaaccaaat aatggttgct gctgtaaaaa agacaacaaa cactaaaagc
     1981 ccaataaaaa agaatttctt cattttctaa cgcttccttt gacagtttta atataccaaa
     2041 gtaaatattt taagattaaa cgataaatgt attctaaaat cttaaatatg acaatgataa
     2101 atactaatcc taatcctgaa tatgatatgg caaaaaatac attactaatt gagtccacaa
     2161 ttcctctgga tattgctgat gctaataaaa taattggaca aaacagcagc aaaagtgata
     2221 ttaaaaataa tgctaataca aataatccaa ctatagatac tggtataagg atgacaaaaa
     2281 tagatagaat gcctaaactt aatgaagcag caacagctct tgaaatattt tcaaatgttg
     2341 gtttagattc agcataatca ataacacttc tagcttttgt ttcagctgca acatcttgtg
     2401 ggtcttttaa cttctttaaa atttcccctt catcaacgcc attaagttct tgctcataaa
     2461 aataacgttc atattcatac attttttgat ctcttaaatg cctaggtagc gcatcaagtg
     2521 ctgtttctaa ttcatttaaa taagtaatct tgttcatata ggtcactcat cccaataaaa
     2581 ttattcattc gttttttata actttaagac ctaaagctat ttactaaaaa attcaatacg
     2641 tttaatttct aaatcttgat aaagtaagtt taattttaaa tggttatctc tatcaatata
     2701 ctttaccgat gagaaatcat tgccaacttt tgttctttta tatccgaatc caagattttc
     2761 aatcgcgtta tttatcggcg aaccaattcc cccatcaaaa ttagtatgta tcttttcatc
     2821 tcttactaat attatcccct taacgatgtt atccttttct ctcactttaa cggtaagatc
     2881 agggtgagct ttactgttat aaaatttaaa acgatcaaac ttaatttttt tatttacttc
     2941 atagcctttt ttatcaaact tctcatttaa tttaatatcg tttatttcaa gattagacaa
     3001 atccacagtt ttcgtatgac gatattgata taaaccaatt aatacaccaa aagaacagat
     3061 taaaaataat ataaacacaa cccagcgatt ttgaatacgc actgatgtca ccctttcttt
     3121 gtacttattt aaatatagca taaggtgctt cccgtttcat ccttaacaaa aattaacaaa
     3181 gaatttgcaa ttaatttatt tcattacctt ttattactat aaattggtat aatgttgtaa
     3241 atgctaaagg aggtttttac aatgacaaat ttagaaactt atttcaaaaa tagccaaccc
     3301 ttaaatgaat acattgatgg aatgaagaca aatcaagata ccgttctcag tatctatcaa
     3361 tcatttgagt taccagaaga tgattcacgc atcaaaaaaa taaaagagat gaattattct
     3421 aaagtattag taattactga agactggtgt ggagatgcaa tgatgaatct cccaattcta
     3481 aaacacatca gtgaagcatt aaatttagaa gtacgcgtgt tccatagaga tgatgataca
     3541 aaactgatcg atcaatattt aacaaatggt aaatcacgtg caattcctat cttcgtattc
     3601 ttgaatgatc aattcgaaca agaaacagtt tggggaccaa gagcaaatga agtccaaaaa
     3661 tttgtaactg atgttcgtgc tgataaatta ccgagcaaag atcatccaga ctatgcagat
     3721 ttagaaaaag aaacacactt agttatatca aaccgctata aaactgacgc ttcattttgg
     3781 aaagcagttt ataattccat cctaaataaa ttag
//
LOCUS       DBNENW010000184         3798 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.188, whole genome shotgun sequence.
ACCESSION   DBNENW010000184 DBNENW010000000
VERSION     DBNENW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.188"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..233)
                     /locus_tag="AC1LW8_002479"
     CDS             complement(<1..233)
                     /locus_tag="AC1LW8_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HIP4774240.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTN"
     gene            complement(277..549)
                     /locus_tag="AC1LW8_002480"
     CDS             complement(277..549)
                     /locus_tag="AC1LW8_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HIP4774241.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            complement(559..660)
                     /locus_tag="AC1LW8_002481"
     CDS             complement(559..660)
                     /locus_tag="AC1LW8_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774242.1"
                     /translation="MKFVANKLTLNIFRKFCINNEKEIWIKYEEVFL"
     gene            complement(1199..1276)
                     /locus_tag="AC1LW8_002482"
     CDS             complement(1199..1276)
                     /locus_tag="AC1LW8_002482"
                     /inference="COORDINATES: protein motif:HMM:NF028245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HIP4774243.1"
                     /translation="MLSFGMFIVTFIGIVVAIINLSNKK"
     gene            complement(1517..2119)
                     /locus_tag="AC1LW8_002483"
     CDS             complement(1517..2119)
                     /locus_tag="AC1LW8_002483"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP4774244.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(2134..2310)
                     /locus_tag="AC1LW8_002484"
     CDS             complement(2134..2310)
                     /locus_tag="AC1LW8_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HIP4774245.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2509..3495
                     /locus_tag="AC1LW8_002485"
     CDS             2509..3495
                     /locus_tag="AC1LW8_002485"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIP4774246.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(3576..3794)
                     /locus_tag="AC1LW8_002486"
     CDS             complement(3576..3794)
                     /locus_tag="AC1LW8_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HIP4774247.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1376 a          573 c          544 g         1305 t
ORIGIN      
        1 ttgtttgtat aatttgaagt tgatttatag attgctacgt tctcattttt agcgaattga
       61 tttatacgtt tataaatctc atttttacta tattttttat tccagtcagt taccgctata
      121 gggtatttag tatcaggcaa tatttcttcg ttttctttat atgtataaac aaataacaat
      181 atagcaatca gaatgaaggt tgtaacatca agtattaatt tgaaccattt catgttgtac
      241 ctcttgcgtt gtttattttc tatgattttt tagtatttaa taaatgttat aatacgctct
      301 attgacccag caattatgtt tagtagctga tgcttttgct tttacaccag ctcttgtata
      361 accactaaat gatctatatt ttccaatagc tgttgcacca tgtcctttat gaccatgata
      421 ataataagac caaacatact tactgccgac accgtgactc caagtgcctc cttctgcgta
      481 ttctgtagat gcataagctg ctgttgataa tccaagtgct aaagttaaat ataatatagt
      541 gccaaaaatt ttattcttct ataaaaacac ctcttcatat ttaatccata tctctttttc
      601 gttatttata cagaattttc tgaaaatatt caaggttaat ttatttgcta caaatttcaa
      661 ccactttatg ttaaatggac atcaaagtat aattgtatta ttgttaaatt gcgcagtgca
      721 taaatagacg acatgcagga acatgtcccc tattgaaccc gttaaaaaga cggtgactaa
      781 atgagatttt ctttaaccat cattcgttgt caaagttttg aaatgatggt tgttttttat
      841 tgtttaaatt aatcattgcg acttctatat caatgaaagt tatattaata tatgaacttc
      901 attacatatt caatagaaat cacttattta taagtactac catataatca taattgattt
      961 ctaccacagt atgattttcc tttcaaatct gacatgttta gcaggcaata tacggaaaat
     1021 acttcaaaat tttgttacac atctaataaa aatagacaat taaataaaag atatgttact
     1081 ataaaaatgt aaaaagacga catgcaggaa catgtcgcct aatgagcccg ttaaaaagac
     1141 ggtgactaaa tgagattttc tttaaccatc attctatgtc aaagttttga aatgatggtt
     1201 attttttatt gcttaaattt attattgcta ctactatacc aatgaaagtg acgataaaca
     1261 taccgaaact taacattaaa tgtaatgcgt ttgcaataga tatcacaata ggcttttcct
     1321 ttcaaaagtg ttaagtaatg ccataggcac cacccccctt tactcagata gccaccatca
     1381 attcaacttg cccacaaaat attatactat ttttataaaa tattaccact ataatgaagc
     1441 atgcctcgaa tcttcgtatt gctattctta agcaaaaaag cttttcttag aacacatact
     1501 tctaagaaaa gcttatttaa gcattaaaaa ttaataacaa tataatagct acaatttgtg
     1561 caacggatgt tgttgctata cgtcgagtat actcataact taatgataat atcacttgtg
     1621 caacaaatat aaatgttact atccaccaat tatctaaata aataaataat gaactagaac
     1681 aaattgcagt tattaatata gctacccatt tcggtattga aaaatgtgtt aattcttttt
     1741 gtaaaacagt tctcatatat aactcgtgac atggaatgac aaaaagtaaa gttactaaca
     1801 tttgccactt ccaattgaca cctgttcttg ctaattcttt aattaatgaa ctataactta
     1861 aattagaagt tataagtgag aataaaagtt gaagcataat taaaataatt gctgtaataa
     1921 taccaacacc aattgatgta agcaaccgct tagattcaat atctctttga taaaatacat
     1981 aactgatacc agcaattagc attatacctg tatataaata ccagtattct tggtgttcgc
     2041 cccacataat aaataatatg agatgaaata taatgaaact tataataaac caagttaaag
     2101 ccttcgagat tttattcatt ataacttacc ttcttattca acaatcttat atcgtttatg
     2161 attgataaca gttttttccg gtaaatcaag gtcattaaaa ttttccataa ttgttaagtc
     2221 aacaataaca gagttatcgt tgattttttc aacacgacct cttaatccat ccataaactc
     2281 aacaatgtta cccacttctg caacagtcat tttaagtcct ccttaaatca taactaaatt
     2341 atattgtact aaaattcact taacaaataa actattaaca atcaaaaata ataaaatgta
     2401 actttaagcc gatatttaga atgatttgtg agtatataat tggcattttg agcaaagtta
     2461 aggcataaat aagtagagaa tatatataga tataagggag agattgtaat gaaattcatt
     2521 agtaataata atattacaga tccaacttta aacttagcaa tggaagaata tgttttaaaa
     2581 aatttaccag cagaagaaag ttacttttta ttttacataa atagaccatc tatcattgtt
     2641 ggaaagaatc aaaatacgat agaggaagta aatcagacat atatagatgc tcataacatc
     2701 gatgtagtaa gaagaatttc tggtggtggc gctgtttatc atgatactgg caatttaaac
     2761 tttagtttca taacagatga tgatggtaac agtttccaca atttccaaaa attcactgaa
     2821 ccaattgttc aagcattaca atctttaggc gtcaatgctg aattaactgg tcgtaacgat
     2881 attcaagttg gacaagcgaa aatctcaggg aatgccatgg ttaaagttaa aaatagaatg
     2941 tttagtcatg gtacgttgat gttaaatagt gatttagacg aagttcagaa tgcactaaaa
     3001 gttaatccag ctaagattaa atcaaaaggt attaaatctg ttcgtaaacg agtagcaaac
     3061 attcaagaat ttttaaatga ccctttagaa attgaagaat ttaaaaaaat catcttgaaa
     3121 actatctttg gagaaactga agtagaagaa tataaattaa cggatgaaga ttgggaaaat
     3181 attgaaaagt taagtaacga caaatataga acttgggaat ggaattatgg tagaaaccct
     3241 aaatataact ttgaacgcga ggaaaaattt gaaaaaggat ttgtacaaat taagtttgat
     3301 gttaaacgag gtaaaatcga acatgcaaaa attttcggtg atttctttgg tgtcggagat
     3361 gtcactgatc ttgaaaatgc attagttggc tgtctacacg actttgagca catagaagaa
     3421 gcactatcag aatatgattt atatcactat ttcggtgaca tagatagaca tgaattaatt
     3481 agattaatgt cataaacgat taaaggacta ttcttacatg tatgagatgt taagaatagt
     3541 ccttttatga ttgcgttatg gaattcgaat caatgttatt caccgagaaa tgcctccaaa
     3601 tttttgtatt ctgccgtata ttgattaaat gctgcttcat ctttattgac gagtgcgtca
     3661 tctataagtt ctatgagctg tcgttttctt acctctcgaa gtgcttcatt gataattaat
     3721 tcaataccca aatcattgac ggttgtgaca aacgcttcta aagttgtatg tttaacatta
     3781 gtattgtatt tcatagaa
//
LOCUS       DBNENW010000185         3754 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNENW010000185 DBNENW010000000
VERSION     DBNENW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.189"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            182..532
                     /locus_tag="AC1LW8_002487"
     CDS             182..532
                     /locus_tag="AC1LW8_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HIP4774248.1"
                     /translation="MNDLVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            525..1088
                     /locus_tag="AC1LW8_002488"
     CDS             525..1088
                     /locus_tag="AC1LW8_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HIP4774249.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1102..1446
                     /gene="gpsB"
                     /locus_tag="AC1LW8_002489"
     CDS             1102..1446
                     /gene="gpsB"
                     /locus_tag="AC1LW8_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIP4774250.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1509..1888
                     /gene="rnpB"
                     /locus_tag="AC1LW8_002490"
     ncRNA           1509..1888
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1LW8_002490"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2097..3242
                     /locus_tag="AC1LW8_002491"
     CDS             2097..3242
                     /locus_tag="AC1LW8_002491"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIP4774251.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            3326..3658
                     /locus_tag="AC1LW8_002492"
     CDS             3326..3658
                     /locus_tag="AC1LW8_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HIP4774252.1"
                     /translation="MGKKMGLGLSIALIVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         1406 a          490 c          716 g         1142 t
ORIGIN      
        1 aagtattttc atcgtataac aacatacacg ataaaatttt gtatttatat aggaagtttt
       61 tagatttact tgatttaaca gataacacat ttgaaaaaat agttaaatca aattatatag
      121 agtgttaaaa catgacgaat acagtatatt gtgataaatt aaaaatgtag gaggcgtcct
      181 catgaatgat ttagtcgaat cactaattta tgaagttaac aacatgcaac aaaattttga
      241 aaatgtgaaa tcacaacaac aagatcatga tttttaccaa actgtaaagc catatactga
      301 acatattgat agcatgctca atgagatcaa attacatcgt gaatttatta tagaagtacc
      361 ttatatgaat tcaaggaaat ttgagctact gattgctaac attgaacaac tttctgtcga
      421 atgtcatttt aagcgaacaa gtcgaaagtt atttatagaa aagcttaaaa gtgttcaata
      481 tgatttacaa aatatattag atggcgtaac aaaagagggt actgatggtt aaaacagttt
      541 atgtaacagg ttacaaatca ttcgaattaa acatttttaa agatgacgca cctgaagtac
      601 attatttaaa acaatttata aaacataaaa ttgaacaact gttggatgaa ggattagaat
      661 gggtgttaat acaagggcaa atgggtatag aattatggac tgcagaagtt gttattgaat
      721 tacaacgaac atatgattcg ttaaagtttg ctgtaattac accgttccaa ggacatacag
      781 aaaagtggaa tgaacataat caaagtaagt atgccaacat aattaagcat gcagattatg
      841 ttgatagtat ttttcatacg tcgtatcagg gtccttttca atttaaacaa gcagatcaat
      901 ttatgcttga acattcggat caaacattgc tcatttatga tgaggaacaa gaggcaagtc
      961 ctaagttctt caagcagatg ttagttgatt ttatggataa aacaaactat acttgtgata
     1021 ttgtgacgtt cgatgaatta acagcattca tcaatgactt acagtggtct gaagatcaaa
     1081 gtttctaatg aggtggaaaa aatgtcagat gtttcattga aattatcagc aaaagatatt
     1141 tatgaaaaag attttgaaaa aacgatggct cgtggctata gaagagaaga agtagatgca
     1201 tttttagatg acattattgc tgattatcaa aaaatggccg atatgaataa tgaagttgta
     1261 aaattatcag aagagaatca taaacttaaa aaagaattag aagaattaag actacgtgta
     1321 gcaacatcaa gacctcagga caataaaagt ttttcttcga ataatacaac aacaaataca
     1381 tcttcaaata atgtagatat tttaaaacgt atttcaaact tagaaaaagc tgtatttggt
     1441 aaataattac taaatacaaa agtttaactg tcttactaat aatgactatg ttataatttt
     1501 aaaagtgata ttttgggtaa tcgctatatt atatagagga aagtccatgc tcacacagtc
     1561 tgagatgatt gtagtgttcg tgcttgatga aacaataaat caaggcatta atttgacggc
     1621 aatgaaatat cctaagtctt tcgatatgga tagagtaatt tgaaagtgcc acagtgacgt
     1681 agcttttata gaaatataaa aggtggaacg cggtaaaccc ctcgagtgag caatccaaat
     1741 ttggtaggag cacttgttta acggaattca acgtataaac gagacacact tcgcgaaatg
     1801 aagtggtgta gacagatggt tatcacctga gtaccagtgt gactagtgca cgtgatgagt
     1861 acgatggaac agaacatggc ttatagaaat atcactacta gtttagctct cctagatgat
     1921 ggagagcttt tttcatgaaa agaacactta aaattaacgc cttgtcttga tataatgaca
     1981 ctgccttgtt ttaaaatagt aagcggatgc gttaatgtat cagcgattaa atttgttgga
     2041 aatgtataaa aaacacaagc taagaataaa atacctgtat aaaaggagaa tcatatatgt
     2101 ttcaattact tgcagtttgt ccgatgggat tagaagctgt tgttgctagg gaaattcaag
     2161 aattaggcta tgaaacaaat gttgaaaatg gtcgtatatt ttttgaagga gacgcaagtg
     2221 caattgtaaa ggcaaattta tggttgcgca cagcagaccg aatcaaaatt gttgttggac
     2281 gttttaacgc aacaacgttt gacgaattat tcgaacaaac caaagcgctc ccttgggaat
     2341 ctataattga taaagaaggt aacttcccag ttcaaggtag aagcgttaaa tcaacactac
     2401 atagtgtgcc agattgtcag gcgataacta agaaagctat tgttgaacga ttacgtcgcg
     2461 catataacga aaaaggttgg ttaaatgaat caggtgccaa ataccctgtt gaagttgcca
     2521 ttttaaaaga taatgtatta ttgactatag acacatcagg ttctggtttg aacagacgtg
     2581 gatatagatt agcacaaggt gaagcaccaa ttaaagaaac gttggcagca agtttaatcc
     2641 gtcttgcaaa ctggaaaggt gatacacctt taattgatcc attttgcggt tcgggtacaa
     2701 tagctataga agcttgttta attgctcaaa atatcgcacc tggttttaat cgcgagttcg
     2761 tatcagagca atggaacatc atgccagcaa atatttatga tgattaccgt gatgaagcgg
     2821 ataagatggc tgattatgat aaagaaatcg aagtatatgc ttctgatatt gatccagaaa
     2881 tggtagagat tgctaagcgt aacgctgaag aagttgggtt gtctgatatt attaaattta
     2941 gtgtaaaaga tgtcaataca ttaacaattg atacagaaga accggtggcg ttaattggaa
     3001 atcctccata tggtgaacgt attggtgatc gtgaagaagt tgaagaaatg taccgttata
     3061 ttggtaaact aatgaaacaa catccatttt tatctacata cattttaaca agtaataaag
     3121 aatttgaata tttagtagat cgtaaagcaa cgaagcgacg taagttattt aatggatata
     3181 tagaatgtac ttattatcaa tactggggta aaaagacaga acgtaaaact atcgaaaact
     3241 aaagtgtttc taatgcgtga ctaaaattag tattaattat gttctcatga taataggtgt
     3301 ttttgaaaaa tggaggagtc tataaatggg taaaaaaatg ggtctaggtt tatctatagc
     3361 attgattgtt attggtattg ccgttgtatg tttaatgatt ttttctagtc aaaaaacgac
     3421 ttattttggt tatatgaata gtaatacaaa tgcagaaaaa gttgtaagtg aaaaagatgg
     3481 attagtcaaa cataatatca aagtagaacc atctaatgat ttcaagccga aaaaaggaga
     3541 ctttgtaaaa ttagtttcta aagatgatgg gaagacattt tataaacaag agattgttaa
     3601 acatgatgac gtcccacacg gtttaatgat gaaaattcac gacatgcata tgaattaata
     3661 aaaaagcatc tataacgtaa ttttgaagaa gtagagttat cttcttatgc gttttagatg
     3721 cttttttatc ttcaaattca aaaaatcgga caga
//
LOCUS       DBNENW010000186         3745 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNENW010000186 DBNENW010000000
VERSION     DBNENW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.190"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            131..433
                     /locus_tag="AC1LW8_002493"
     CDS             131..433
                     /locus_tag="AC1LW8_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774253.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            689..1054
                     /gene="crcB"
                     /locus_tag="AC1LW8_002494"
     CDS             689..1054
                     /gene="crcB"
                     /locus_tag="AC1LW8_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIP4774254.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            1051..1404
                     /locus_tag="AC1LW8_002495"
     CDS             1051..1404
                     /locus_tag="AC1LW8_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter FluC"
                     /protein_id="HIP4774255.1"
                     /translation="MISIILVMIGGGFGAITRSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLNIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(1787..2620)
                     /locus_tag="AC1LW8_002496"
     CDS             complement(1787..2620)
                     /locus_tag="AC1LW8_002496"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4774256.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(2832..3740)
                     /locus_tag="AC1LW8_002497"
     CDS             complement(2832..3740)
                     /locus_tag="AC1LW8_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HIP4774257.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVTLGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
BASE COUNT         1180 a          655 c          515 g         1395 t
ORIGIN      
        1 atagctaatt ttttgcctat atttttaaaa aatgacttta gtaaacattg tgtctacaag
       61 ttaataaaac tattgtttta tgattgttta tgatacgata tgtaaatgat aaccatccag
      121 gaggtctagc atgtctcgtt caaaaaaata cttttactta tctagcttaa tgattatttt
      181 aagctttttc tttaatacaa ataacgtttt cctaagtgga ctttttaatt cttttattaa
      241 attaatactt ttctgcagtg ttattaactc aattgtacta attttgtcta taatttttgc
      301 agatcgttca attaaatcac taaagcctga tgcagattgg attagaattg cgagtaaaag
      361 tttgccttgg attattctaa ttgttatttt agtacatatc ttttcaattg ttcgtacatt
      421 cggttttatt taaaaaagat aaaatgtcaa tgtagcctta atacaaaaca atacaatgta
      481 tcatgctata atgagtaaaa caatttgatg acgttgttgc gtataaaaaa tattagattt
      541 tcgaaatcat aactatacat ctaatcgcta tagttataca acaagatata acatataatg
      601 aggtttgata atgcatcgac aatttttgtc gtcgcgttgc caaaacctct tttttaaatt
      661 caaactactt cttttcgagg tgaaccaaat gcaatatgta tatattttta tcggtggtgc
      721 tttaggcgct ttattacgtt acctcatttc ttttctgaat actgacggag gttttccaat
      781 cggaacactg atagccaatt tgactggtgc ctttgtaatg ggattgctaa cagccttaac
      841 aattgcattt ttttcaaacc atccgacgct aaaaaaagct attacgactg gttttcttgg
      901 tgctttaacg actttttcaa catttcaatt agaattaata catatgtttg atcatcaaca
      961 atttataact ttactactat atgctgtaac aagttatgtc tttggtattt tgttatgtta
     1021 cgtcggtata aaactaggtg gtggtttatc atgatatcaa tcattttagt catgattggc
     1081 ggcggtttcg gtgcaattac tagaagtgcc attactgatt attttaatca taaatttact
     1141 tcaaagttac ctatcgcaac attgatagta aatctagttg gtagtttttt aattggatta
     1201 aatataggct tatcaatttc aatctcatgg ttccctgcgt tctttgttac cggtttttta
     1261 ggtggcttaa caactttctc aacgttagcc aaggaactta cactaatgat gacgccaaaa
     1321 tttaatatta acctttttct caattattca cttttacaat tcatcattgg atttatagct
     1381 tgttatattg gctatcatat ttaaaaataa aatgcttcat tcagcaaata ggtaaattac
     1441 gacaccttcc tgaacgaagc attttttaat tttcatgcaa attttttagc accataaaat
     1501 gcctaccaaa tttcaataat ctttgttacc gtttaaataa tgtgaatgtc aataaattct
     1561 ccaaactagt cgaaaataaa gggagtggga cagaaatgat attttctcaa aatttatttc
     1621 gtcgtcccac cccggcaagg ttgactagaa ttgaaaaaag cttgttacaa gcgcattttc
     1681 gttcagtcaa ctactgccaa tataacttcg tagagcatag aacattgatt tatgtcccag
     1741 cctgtgacaa tccatctaac cttttcatca gttgagtgat tttaatctat ccttcaaaag
     1801 tttttggatc aggtccaatt ctcttatctt gatttaaacc atcaattcgc gtcatttgtt
     1861 catctgataa ttcgaaatca aatatttgga agttttcaga gattctgttt ggtgttaccg
     1921 atttagggat tgtaaccaca ccatgctgca cattccatct taaaacaact tgggcaggtg
     1981 actttcctaa ttcttgagca atgtctttaa ttgtctcatc atttaaaatt tgtgcattca
     2041 tcaatggtga ccaagattcc atcacgatat gttgtgctgc caaatataat ttcaatttat
     2101 gttgcgttaa atatggatga tattcaacct gattaattac aggtttaatt gacacttgtg
     2161 ccaacaaagc ttccaaatgt tcaggttcaa aattgctgac acctatattt ttaactttat
     2221 tatttttata taaatcttcc atacctttcc atgtatcaac cattacggct tcgttcgtac
     2281 ctggccaatg tactagatac aaatctaagt attttaaacc taatctagat aaactagctt
     2341 cgtaagcagc tgctacattt tcacgaccga aatcctcaaa atataatttt gaagtaataa
     2401 ataagtcttc tctagcaata ccagttgact ccaatccggc acgaatgcca gcacctactt
     2461 gttcttcatt cccataaact tttgcggtat caatactacg atatccttgt tcaatggcat
     2521 acttaacact ttccatgcaa ttttcatcat tttccacacg aaatgtccct aaaccaattt
     2581 gtggcatcgt gtttccatta taaaatgttt taacctccat aaatatcgcc tcaccttttt
     2641 gatgtattat accctgttat cataacaaat ctgagttgaa tacatgagaa aaaacactta
     2701 gagcaatcaa ccactaaaat tctagtaata tctctcaaat attaatcaaa ttgtaaaagt
     2761 aattctgttt aatttatgac aaactaaaaa agccgaagta acaacatata gtcatcactt
     2821 cagcctaaca tttaattgaa tgattcaatt ttatccatca tttgttgtaa gtcttccacg
     2881 ttgtattgaa tacgaccatg gaatacaaat ttgttaaaga actcgtctaa ttgttcagca
     2941 ccgacaagca ctttgacagc actattttga ttataatttg aaatcgttac atcgccttca
     3001 tttttaggat taaagtataa aattgaagtt ggcgtatatt tggcacctaa ttctttttgt
     3061 aagtcttcag ccaattgttt aatcgcctca atttgatctg aataatttac aaaagataat
     3121 gaacgtttgt catcattttg atccatcaca atagtttgtg gtctagattt atctaaatcc
     3181 aatgtatcaa atacttgttc cattggtggt aaatctttaa attgaccgcc actaatacca
     3241 ttataaacat gaccttttaa caattgagaa tcaataatat aaagaccagt tcttgttaat
     3301 actaaatgac taattcgttc aatattatta aagccatcct ttggtaaaaa gatatttgcc
     3361 ataatgtgca tatcttctgg tcgaattcgt ttttctttaa ctaatctttc acgaatacca
     3421 attaatctca tgtccgttac atattcacta tgatttttcg agaacaattt taatgcgtca
     3481 atctcacgat cttttgtact aaccatgtga ttataatctt cttgttgttt tgtaattgtc
     3541 tttttatttt gaatacgctc tttctctaaa gcttcttcat gagacttttt aatgttttgt
     3601 tcttgttgtt catacttttc ttctgtttgt cgcttaactt ttttcttact acctaaggta
     3661 actaagaaaa ggacaaaaaa gattaatgca atgactactg caataatgag tccaatgact
     3721 atcggtgaag ataaatccat cacaa
//
LOCUS       DBNENW010000187         3741 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNENW010000187 DBNENW010000000
VERSION     DBNENW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.191"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            124..2199
                     /gene="topA"
                     /locus_tag="AC1LW8_002498"
     CDS             124..2199
                     /gene="topA"
                     /locus_tag="AC1LW8_002498"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIP4774258.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2355..3662
                     /gene="trmFO"
                     /locus_tag="AC1LW8_002499"
     CDS             2355..3662
                     /gene="trmFO"
                     /locus_tag="AC1LW8_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIP4774259.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1439 a          549 c          729 g         1024 t
ORIGIN      
        1 tattttttaa taaaatctat aaaaaataga cagggaaaat gatttgttta gatataaaac
       61 gttgacaaaa gcaaaattaa gcgtttatca tttatcttta gtaattagat tagcgagggg
      121 gaaatgacat tggcagataa tttagtcatt gttgaatcgc ctgcaaaagc aaaaaccatt
      181 gaaaagtatt taggtaagaa atataaagtt atagcttcaa tgggacacgt cagagactta
      241 ccaagaagtc aaatgggtgt cgacactgaa gataattacg aaccaaaata tataacaata
      301 cgcggaaaag gtcctgttgt aaaagaattg aaaaaacatg caaaaaaagc gaaaaacgtc
      361 tttctcgcaa gtgaccccga ccgtgaaggt gaagcaattg cttggcattt atcaaaaatt
      421 ttagagcttg aagattctaa agaaaatcgc gttgttttca acgaaataac taaagacgct
      481 gttaaagaaa gttttaaaaa tcctagagaa attgaaatga acttagtcga tgcacaacaa
      541 gcgcgtcgaa tattagatag attggttggc tataacatct cgccagttct atggaaaaaa
      601 gtaaaaaaag gtttgtcagc gggtcgagtt caatctgttg cacttcgttt agtcattgac
      661 cgtgaaaatg aaattcgaaa ctttaaacca gaagaatatt ggactattga aggagaattt
      721 agatacaaaa aatcaaaatt caatgctaaa ttccttcatt ataaaaataa accttttaaa
      781 ttaaaaacga aaaaagatgt tgagaaaatt acagctgcac tagatggaga tcaattcgaa
      841 attacaaacg tgactaaaaa agaaaaaacg cgtaatccag caaacccatt tacaacttct
      901 acattacaac aagaggcggc acgtaaatta aactttaaag caagaaaaac aatgatggtc
      961 gcacaacaat tatatgaagg tatagatttg aaaaaacaag gtacgattgg tttaataaca
     1021 tatatgagaa ccgattctac acgtatttca gatactgcca aagctgaagc aaaacagtat
     1081 ataactgata aatacggtga atcttacact tctaaacgta aagcatcagg gaaacaaggt
     1141 gaccaagatg cccatgaggc tattagacct tcaagtacta tgcgtacgcc agatgatatg
     1201 aagtcatttt tgacgaaaga ccaataccga ttatacaaat taatttggga acgatttgtt
     1261 gctagtcaaa tggctccagc aatacttgat acagtctcat tagacataac acaaggtgac
     1321 attaaattta gagcgaatgg tcaaacaatc aagtttaaag gatttatgac actttatgta
     1381 gaaactaaag atgatagtga tagcgaaaag gaaaataaac tgcctaaatt agagcaaggt
     1441 gataaagtca cagcaactca aattgaacca gctcaacact atacacaacc acctccaaga
     1501 tatactgagg cgagattagt aaaaacacta gaagaattga aaattgggcg accatcaact
     1561 tatgcaccga caatagatac gattcaaaag cgtaactatg tcaaattaga aagtaagcgt
     1621 tttgttccta ctgagttggg agaaatagtt catgaacaag tgaaagaata cttcccagag
     1681 attattgatg tggaattcac agtgaatatg gaaacgttac ttgataagat tgcagaaggc
     1741 gacattacat ggagaaaagt aatcgacggt ttctttagta gctttaaaca agatgttgaa
     1801 cgtgctgaag aagagatgga aaagattgaa atcaaagatg agccagccgg tgaagactgt
     1861 gaagtttgtg gttctcctat ggttataaaa atgggacgct atggtaagtt catggcttgc
     1921 tcaaacttcc cggattgtcg taatacaaaa gcgatagtta agtctattgg tgttaaatgt
     1981 ccaaaatgta atgatggtga cgtcgtagaa agaaaatcta aaaagaatcg tgtcttttat
     2041 ggatgttcga aatatcctga atgcgacttt atctcttggg ataagccgat tggaagagat
     2101 tgtccaaaat gtaaccaata tcttgttgaa aataaaaaag gcaagacaac acaagtaata
     2161 tgttcaaatt gcgattataa agaggcagcg cagaaataat atttttattt cctagataca
     2221 ttttaaggtt gttaaataga atcattagtg aatcttattt taaagatagt aatggattaa
     2281 tctaaataag tgcggataat ataaacataa caacataatt aaaagacata aatgacaata
     2341 aaaggagtat agaaatgact caaactgtaa atgtaatagg tgctggtctt gccggttcag
     2401 aagcggcata tcaattagct gaaagaggaa ttaaagttaa tctaatagag atgagacctg
     2461 ttaaacaaac accagcgcac catactgata aatttgcgga acttgtatgt tccaattcat
     2521 tacgaggaaa tgctttaact aatggtgtgg gtgttttaaa agaagaaatg agaagattga
     2581 attctataat tattgaagcg gctgataagg cacgagttcc agctggtggt gcattagcag
     2641 ttgatagaca cgatttttca ggttatatta ctgaaacact taaaaatcat gaaaatatca
     2701 cagttattaa tgaagaaatt aatgccattc cagatggata cacaattatc gcaacaggac
     2761 cacttactac agaaaccctt gcgcaagaaa tagtggacat tactggtaaa gatcaacttt
     2821 atttctatga tgcggctgct ccaattattg aaaaagaatc tattgatatg gataaagttt
     2881 acttaaagtc ccgttatgat aaaggtgaag ctgcatattt aaactgtcct atgactgagg
     2941 atgaatttaa tcgcttttat gatgcagtat tagaagctga agttgcgcct gtaaattcat
     3001 ttgaaaaaga aaaatatttc gagggttgta tgccttttga agtaatggca gaacgcggac
     3061 gcaagacatt actatttgga ccaatgaaac cagtaggatt agaagatcca aagactggga
     3121 aacgtcctta tgcagtggtt caattaagac aagatgatgc tgctggcaca ctctacaata
     3181 ttgtcggctt ccaaacgcat ttaaaatggg gagctcaaaa agaagttatt aaattaattc
     3241 caggtttaga aaatgttgat attgttagat atggtgtgat gcatagaaat accttcatta
     3301 actcaccgga cgtattaaac gagaaatatg aattgatttc acaaccaaac atacagtttg
     3361 cgggacaaat gactggtgtt gaaggttatg tagaaagcgc agctagcggc ttagttgcag
     3421 gtatcaatct tgcgcataaa atattaggca aaggtgaggt agtatttcct agagaaacga
     3481 tgattggaag tatggcttac tatatttctc atgctaaaaa taataagaat ttccaaccta
     3541 tgaatgctaa cttcgggtta ttaccatctt tagaaactag aattaaagat aaaaaagaac
     3601 gctatgaagc acaagctaat agagctttgg attacttaga aaatttcaaa aaaactttat
     3661 aaaatagtta gaaagactag atatgctatt cattcttaag tcatcaacga gtaagtaatg
     3721 actttctaaa tggaaaatac t
//
LOCUS       DBNENW010000188         3708 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNENW010000188 DBNENW010000000
VERSION     DBNENW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.192"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            203..853
                     /locus_tag="AC1LW8_002500"
     CDS             203..853
                     /locus_tag="AC1LW8_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HIP4774260.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNRSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            934..1929
                     /locus_tag="AC1LW8_002501"
     CDS             934..1929
                     /locus_tag="AC1LW8_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIP4774261.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSIAPTNAKGTFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QNDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAAASSNSDNVSYEASATTPATTSSANNDSEENEQSSKDED
                     KQNASKSDKSSVEKSESNEETATVESAPQSKPVTSEAPPNQNNHNEDSMYDASTE"
     gene            2003..2629
                     /locus_tag="AC1LW8_002502"
     CDS             2003..2629
                     /locus_tag="AC1LW8_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774262.1"
                     /translation="MKFKAIVAIALSLSLLTACGANQHKENNSKSNDTNKKTQQTDNT
                     TQSNTDKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDETDGESEDI
                     QHHDNDNNKAIQNDIPDQKVDDKSDKNAVNKEEKHDNGTNNSEETKVK"
     gene            2670..3011
                     /locus_tag="AC1LW8_002503"
     CDS             2670..3011
                     /locus_tag="AC1LW8_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HIP4774263.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSSLGMKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIMQK"
     gene            3112..3684
                     /locus_tag="AC1LW8_002504"
     CDS             3112..3684
                     /locus_tag="AC1LW8_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774264.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSITKGKYTRAGEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYISEYNPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLKEIEIYDFLESR"
BASE COUNT         1520 a          581 c          606 g         1001 t
ORIGIN      
        1 atgttcgtgt cattttgagt cgtaatacaa ctttacaatt attaaattaa cacttctaca
       61 atccaaaaat atttttaaag ttttgaaaaa aattaaagta tttccactac taatttggta
      121 aaatatgatt atgtgatata gtgaaagcgt tttacactta ataactccct cttaaatgca
      181 tccaggtttt atgtagtaaa tcatgaaaat aacatataaa tatagaggag atttaccttt
      241 gaatacagag aacaacaaga atcaaaaccg atctgttaaa aattctgaaa gacgtggcat
      301 gttaaaagga tgcggcggtt gccttatttc tttcatttta ttaataatct tattatcagc
      361 ctgttcaatg atgtttagta ataatgacaa ttccactagt aatcaatcat caaaaacgca
      421 attaactcaa aaagatgaaa ataaaaatga agataagcct gaggaaaaat cagaaacagc
      481 aacagatgag gatttacaat caaccgaaga agtacccgca aatgaaaata ctgaaaataa
      541 tcaacatgaa attgatgaaa taacaacaaa agatcaatca gatgatgaaa ttaacacacc
      601 aaacgttgca gaagataaat cacaagacga cttgaaagat gatttaaaag aaaagcaaca
      661 atcaagtaac catcatcaat ccacgcaacc taagacctca ccatcaactg aaacaaacac
      721 gcaacaatca tttgctaatt gtaagcaact tagacaagta tatccgaatg gtgtcactgc
      781 cgatcatcca gcatatcgac cacatttaga tagagataaa gataaacgtg catgtgaacc
      841 tgataaatat taaacaacaa gcgaattgaa ttcaaattgt atttagcttt atgcactaat
      901 cacatagtaa ataatgaggg agatttttta ggcatgagca atcaattcaa aagcgaagaa
      961 gagcgaagac aatgggaaca attccaagct ttccaaaatc aacaaaacca acagaaccag
     1021 caatacggac aaaagaaatc taaaaaagga tggttctggg gctgtggtgg ttgtctagta
     1081 ttatttattt taattatcat cggtatttca gcttgtacag ctggtattac aggtaacctt
     1141 ggcggaaata gttctaaaga aacgaacaaa acccataaaa ttggagagac tgtaaaaaat
     1201 ggtgatcttg aagtcacagt taattcagtt gaaactatga aatcagttgg tccatctatc
     1261 gcgccaacaa atgctaaagg tacatttgtc gttgctgatg tgacgattaa aaacaaaggt
     1321 aaagaagcgt taacaattga tagttcaatg tttaaactga aatctggtga taaaacattt
     1381 gaagcagata atacaggctc aatgtctgct aatcaaaatg acaacggtag tatagaaaat
     1441 tcatttttct tacagcgtat aaatcctgat agcactgctc aaggtaaaat tgttttcgat
     1501 gtgtcagaaa acatagccaa cgcaaaagat aaaaaattag aagttatttc tagtttattt
     1561 agcgtcaaga agattacatt tgatttatcc gatgctaaaa aaacatcaaa agctaaaaaa
     1621 gacaagcaag atacagaagt agctgctgcg agttcaaata gcgataatgt aagttatgaa
     1681 gcttcggcta ctacacctgc tacaacttct agtgcaaata atgattctga agaaaatgaa
     1741 cagtctagta aagatgaaga taagcagaat gcgtctaaaa gtgataaatc tagtgtagaa
     1801 aaaagtgaat ctaatgaaga aactgctact gtagaatcag cacctcaaag caaacctgtt
     1861 acaagtgaag caccacctaa ccaaaataat cacaacgaag atagcatgta cgacgcttca
     1921 acagaataaa atttcagggt agctcggcta cccttctttt acggaaaaat aagtatacat
     1981 agcaatataa ggagataaaa aaatgaaatt caaagctatc gttgccattg cattatcatt
     2041 gtcactatta actgcttgcg gtgctaatca acataaagaa aataatagta aatcaaatga
     2101 cactaataaa aagacgcaac aaactgacaa cactacacag tcaaatacag acaagcaaat
     2161 gacaccacaa gaagccgaag atatcgttcg aaacgattac aaagcaagag gtgctaacga
     2221 aaatcaaaca ttaaattata aaacaaatct tgaacgaagt aatgaacatg aatattatgt
     2281 tgaacatcta gtccgcgatg cagttggcac acctttaaaa cgttgcgcta ttgttaatcg
     2341 acacaatggt acgattatta atatttttga tgatatgtca gaaaaagata aagaagaatt
     2401 tgaagcattt aaaaagagaa gccctaaata caacccaggt atgagtgatc atgatgaaac
     2461 agatggtgag tcagaagaca ttcaacatca tgacaatgat aataacaaag caattcaaaa
     2521 tgacatacca gatcaaaaag tcgatgataa aagtgataaa aatgctgtta ataaagaaga
     2581 aaaacacgat aacggtacaa ataattctga agaaactaaa gttaaataag ggtatacttt
     2641 gattaatcgt aatttttagg agaaccgcta tgataacatt tgaaaatata caacaacttg
     2701 aaaaatacac cttaatgact atgcatggtc tttttaatca acttaaactc ggtattattt
     2761 caatcgacaa tgcagagcat acgctcttta caccttatat gatggaaaca ctctcttccc
     2821 taggcatgaa agacagcatt gtcgatttaa ttcataaagg gactgaatta gaagactttg
     2881 cagcatttaa tttatcaatt gaagatacag ttacagtctg tttacaaaga actgaagaac
     2941 tattaaatca atacaaaaat gtggaattca atgacaaaat attaatcaat tggcgtatta
     3001 tgcaaaagta atagacatat aaaagtcgaa cgtaactaaa tggatattga ttttattctt
     3061 tctaaattac aagtatttca ttttataaag tttgaaaaga ggtatattga agtggagaaa
     3121 aatgaatata tagctaaata taatgaatat agtcaattat tagacgctac atactcgcaa
     3181 gccgtagcat accttttaaa taaatatggc gctgtaaccg atgactatta taaggaaaaa
     3241 tcatacacgc gatttttaaa tggagaaatc aaaagtatta caaaaggaaa atacactaga
     3301 gctggtgaag gattatattg tcatcacata agcgaagaca aattccaaaa cttatctgac
     3361 ctaagattca tttccgaatt taagtactca tacaactatc aaaagaaaga aaacttagtt
     3421 tactgcgatt taatcgagca tttaatttta catgcaatta ttacaaaaga atccaatggc
     3481 caatttggtg tggctggatt atgtcaaatg atcaaaccaa cagtcattga ttggtacatt
     3541 agcgaatata atccaaaacc agcatggatg caagccacca aagcacgtgc ctatttgcct
     3601 agaatattag tagagaaact actcattaaa attgacgata tgttaaaaga aatagaaata
     3661 tatgatttcc ttgagtctag ataaatgatt aatggagatt tatttttt
//
LOCUS       DBNENW010000189         3681 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNENW010000189 DBNENW010000000
VERSION     DBNENW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.193"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..327)
                     /locus_tag="AC1LW8_002505"
     CDS             complement(<1..327)
                     /locus_tag="AC1LW8_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774265.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETLYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTQLSNAL
                     FAIKFAH"
     gene            376..1113
                     /gene="sdpB"
                     /locus_tag="AC1LW8_002506"
     CDS             376..1113
                     /gene="sdpB"
                     /locus_tag="AC1LW8_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase
                     SdpB"
                     /protein_id="HIP4774266.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1204..1563)
                     /locus_tag="AC1LW8_002507"
                     /pseudo
     CDS             complement(1204..1563)
                     /locus_tag="AC1LW8_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1760..2452)
                     /locus_tag="AC1LW8_002508"
     CDS             complement(1760..2452)
                     /locus_tag="AC1LW8_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4774267.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2837..3634
                     /locus_tag="AC1LW8_002509"
     CDS             2837..3634
                     /locus_tag="AC1LW8_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIP4774268.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATYFPEHQLLAEEKSNAMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
BASE COUNT         1254 a          490 c          638 g         1299 t
ORIGIN      
        1 atgggcaaat ttaattgcaa ataatgcgtt agatagttgt gtatgataaa aattaagata
       61 ttgaattttg ttaccatagt cgacttgtcg gaggggacgt ttattaagta gtgttataat
      121 ttcataatag gaaacttgat tttcgtttat tatatataat gtttcaaagc ctttgaactt
      181 attcaattca ttagtaacta tttggttaaa tttataaata tgcgtttgaa tgttccaatc
      241 tgtcattaag gtattatatt taatcgtatc aatgatttga atcaaattag taatttgttg
      301 tctcatatac ttagtttcgt ttatcatgta gaagtacctc caaaaatcaa ttattaaaca
      361 ggaaaggata taacagtgga aaatgaaaaa aagaaataca cgtttaaaga tatagcatgg
      421 agagatttgc ttataattcc aattataggt gttatatttt atgcactcac aattgcagct
      481 tacataggac caattttgtt tggttattat gaaatcgata gtaatctgat atttgtagcg
      541 actatcacac aagctttagc atatacgatt gggattggcg cattttattt atttcattta
      601 aaagtgatgc catcaaggct tcgagctggt tttgcgtata tcaaaaaata ttggcttaga
      661 ttgtcaataa cgtatattat tgctctcatc ttaatttata catatgaata tatgacacag
      721 tttctaccta aacatttgca atatagtgaa acagccaatg aacttgagct aaataaaatg
      781 tttgaagtgc ctgcattttt acctgtagca ttcttattaa ttgtgattgt aggtccaata
      841 gttgaagaaa tcgtatttag acatatttta attggagaat taggtaagaa gtttaatttt
      901 atagctatga gtattgtttc ggtgttctta tttgcattta ttcatgtgac tgatgcaaag
      961 tcgccgtttg agtttggtcc atatctcata ctatccatca tattagtttt cacttattta
     1021 aaatctggaa gaaacctagg ttcaacaatt gcattacata tagcaaataa ttttgtttct
     1081 ttcgttatat cagtaataag tatttatgcg tagataaaga ggtcgggaca ttaagttttt
     1141 aggcaatgaa aaaagctgat ttctattaat tatttgatag aaatcagctt ttttgatatg
     1201 cattttatta tgaaactgaa taaataaaga ttcaattagc gcatcagtat taggattcac
     1261 tctaaaaaga ttaatagttt tataagaagg tgtttgatct tgagctaacc acatcattcg
     1321 aatactgtta tgaagtaatt tttctattct acggccagaa aattcagatt gagtatatga
     1381 atatgaggtg atttttaaca tcatttttag atgataggat gttgcgccac gatgatgtct
     1441 gaattcatcg aattcgctat cagctatctt tttaacaatt tcattaacat atcgcgaaat
     1501 atcattttga ggaattctaa cagcagtttc tattggtagt gtaagttggg caaagtgttt
     1561 tattttttta agtatttcaa agtaaaatta catgttaata cgtagtatta atggcgagac
     1621 tcctgaggga gcagtgccag tcgaagacca aggctgagac ggcaccctag gaaagcgaag
     1681 ccattcaata cgaagtattg tataaataga gaacagcagc aagatatttt ctaattgaaa
     1741 atcatcttac tgctgttttt taggaatcga tggggtttaa aaattgatga tagttatcaa
     1801 tggatatgtc tctggtttga atcgtcttac cgaatgaata cgaaatatct ataatatttt
     1861 caatttcaag ttcggcagtt ttgtcttgag caatgtcata aatgactgca tgccaatgtc
     1921 catgtttaca caaaaggcca ataaatataa cgttttcagc tttaatatta tcatcaaccc
     1981 tatgatctac taacatcaca tttcgttctg tacaataaat taaaatgtcg gaaaacagca
     2041 ttgggagtgt catatgttgt gtgtcatcaa atttaatata aaagtccatg cgtttaagca
     2101 attttcttaa gcgtgtttgt ggaagactta aacattgttt tataattgcg ttgatttcgc
     2161 ttttatatgg taatgtcgag tacgactgac tttcattaag cactaaaaat aaggccgata
     2221 attgtgtttc agataagttt aatgcaattt tagattgtgc ttgttgtatt tggtaaccac
     2281 catttttccc ataatgcgca taaattttaa caccttgatt ttctaaatca tcaatatctc
     2341 ttaaaattgt gcgtttggat acgttgcaat atttagctaa ttctaaagcg gtcatttttt
     2401 tattttgttg aattgcagta attattaaat tttgcctttc agctttattc atgacagcca
     2461 cctcctaaaa gatgatatat tacacaaagt ataacataag agtgacaatg aggacatttt
     2521 caatatcttt taaaaaaaat cgtaaaaatt ttgtggaaaa cgcttacatt ttaaaaaaga
     2581 ttgtgctatg atattacttg taacaggggg agcgattaaa caaaggggta gagctatata
     2641 acagatagct tttaatcgtt caagttacat cacaacatct tcaatattta ttacttactt
     2701 tcctttctat ttgtcggcta gcacgtgact agccgatttt tttatgcata aaatgaagaa
     2761 gtcttcagtt gtattgttga ttcttttaat atagggtaat aataaaatga taatgatatg
     2821 agaagtggtg gacgaaatga cagataaaac attacagcaa atagataagt taatatgttc
     2881 gtggttgaaa caaatagaca atgttattcc gcaattaatt atggaaatga ctacagaaac
     2941 taagcgtcat cgatttgatt tagttacaaa tgtagataaa cagattcaac aacaatttca
     3001 acaattttta gcaacatatt ttccggaaca ccaactatta gcggaagaaa aaagtaatgc
     3061 aatgattacg aatgaaatta atcatttatg gattatggat cccattgatg gaactgccaa
     3121 tttagtaaaa cagcaagaag attattgtat tatattggcg tatttttatg aaggtaaacc
     3181 aatgttatcg tacgtatatg attatccgca taaaaagctt tataaagcaa tacgaggaga
     3241 aggtgctttt tgtaatggaa ttaagatgga agaaccacca tcgttaaaat tggaagacgc
     3301 tattatatcg tttaatgcac aagtgatgaa tctagatacg gtgcaagatt tatttgacgc
     3361 ctcatttagt tatcgtttag ttggtgcttg cggattagat tctatgcgag tggcaaaagg
     3421 tcaatttggg gcacatatta atacaaatcc taaaccttgg gacattgctg cacagttttt
     3481 atttgcagaa ctattgaatc taaaaatgac gactttagat ggtaaggcaa ttgatcattt
     3541 aaagggtgca ccttttatta taagtaataa agcatgtcat gaaacggtac ttaaaatttt
     3601 aaatgcaaat ggtggttatc aaaaatacag atagatattc tctagagata aaaaatgata
     3661 aaaaatagtt atacgtctac a
//
LOCUS       DBNENW010000190         3651 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNENW010000190 DBNENW010000000
VERSION     DBNENW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.194"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            138..593
                     /locus_tag="AC1LW8_002510"
     CDS             138..593
                     /locus_tag="AC1LW8_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4774269.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            833..2215
                     /gene="tcaA"
                     /locus_tag="AC1LW8_002511"
     CDS             833..2215
                     /gene="tcaA"
                     /locus_tag="AC1LW8_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein TcaA"
                     /protein_id="HIP4774270.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKTVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2246..2374
                     /locus_tag="AC1LW8_002512"
     CDS             2246..2374
                     /locus_tag="AC1LW8_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774271.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2484..>3651
                     /locus_tag="AC1LW8_002513"
     CDS             2484..>3651
                     /locus_tag="AC1LW8_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIP4774272.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRDIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILII"
BASE COUNT         1339 a          455 c          672 g         1185 t
ORIGIN      
        1 aagcaaggag aacgcagcaa catgtaagtc ttaatttgtt atattatata tgggtcaata
       61 tttttgtgtt ttttagatgt gcgatacaat gattgctgag gatactttat aaaaatagta
      121 attagggaga aataattatg gtaaaacatt tacaagacca tattcaattt ttagagcagt
      181 ttataaataa cgttaacgca ttaactgcaa aaatgttgaa agatttacaa aatgaatatg
      241 aaatttcatt agagcagtct aacgtattag gtatgttaaa taaagaacct ttgacaatta
      301 gtgaaatcac gcaaagacaa ggtgtaaata aggccgcagt aagccgacga attaaaaagt
      361 taatcgatgc taaattagtt aagttagata aaccaaattt aaatattgat caacgtttga
      421 aattcataac cttaactgac aaaggtagag catatttgaa agaacgtaat gcgattatga
      481 cagatattgc gcaagatatt actaatgatt taaattctga agatattgaa aatgtaaggc
      541 aagtattaga agtcatcaat catcgcatta aaacatatag caatcataaa taggtttttg
      601 aaggtgttca aatataagac taaatatgta tgtattgaac accttctttt tagttataat
      661 tgagagagta ctttcataaa taatgtaata aggtacaatt ctaaagtaaa gttaatcatt
      721 tcttgaattg tattcttggg ataatatttg taaaataata atggtaaaat aatgaaataa
      781 tttttgtgat atacatataa caattgaaga ggaacagaat tgaggtgaaa gtatgaaatc
      841 ttgcccgaag tgcggacaac aagcacaaga tgatgtacag atatgtacac aatgtggaca
      901 taaatttgat agtcgtcaag cattatatag aaagtcaacc gatgaagata tacaaactaa
      961 taacatcaag atgagaaaaa tggtgccttg ggcaattggt tttttcattt taatcttaat
     1021 tattatattg ttctttttac taagaaactt caattcacct gaagcgcaaa ctaaaatatt
     1081 agtgaatgcg attgagaata atgataaaca aaaagtagca acattattaa gtactaaaga
     1141 taataaagta gattctgaag aagcgaaagt atacattaac tatatcaaag atgaagttgg
     1201 gcttaagcaa tttgtcagcg accttaaaaa tacggtacat aaattgaata agagtaagac
     1261 cagcgtagct tcttatattc aaaccagatc tggtcaaaat atattacgtg taagtaaaaa
     1321 tggcacgcgt tatatctttt tcgataatat gagctttact gcacctacca agcaaccaat
     1381 tgttaaaccg aaagaaaaaa caaaatatga gtttaaatct ggtggtaaga aaaagacggt
     1441 tatagctgaa gcaaataaag tgacgccaat aggtaatttt ataccgggga catatagaat
     1501 tccagctatg aaatcaactg agaacggtga ttttgcaggc tatttaaaat ttgattttag
     1561 acaaagtaat tctgaaacgg tagatgttac tgaagatttt gaagaagcaa atataactgt
     1621 aactttaaaa ggcgatacaa aattaaatga tagttctaaa aaagtaacta taaatgaccg
     1681 tgaaatggca ttttcaagtt ccaaaacgta tggtccatat ccacaaaata aagatattac
     1741 catttcagct tcaggtaaag cgaaagataa aacatttaca acacagacga agacgattaa
     1801 agctagcgat ttaaaataca atacagaaat aactttgaat tttgacagtg aagatatcga
     1861 agactatgtt gaaaagaaag aaaaagaaga aaacagcttg aagaacaaat tgatagaatt
     1921 ctttgctgga tattctttag cgaataatgc tgcgtttaat cagtcggatt ttgattttgt
     1981 atcatcatat ataaaaaaag gatcatcttt ttatgatgat gtaaagaagc gtgtatctaa
     2041 aggaagttta atgatgatta gttcaccaca aattatagat gctgaaaaac atggtgataa
     2101 gattaccgca accgtaagat taataaatga aaacggtaag caagtagata aagaatatga
     2161 gcttgaacaa ggctcgcaag accgcttgca attaatcaag acatcagaaa aatagagtat
     2221 aaaacttaaa taagatgcat gtgtcatgga atacaaacaa catcctttca aagtatttac
     2281 aattagtgtg aattactttg aagggattgt tttttgtgga aaaatttatg aagtgctgag
     2341 gtttatatca ttttatacct tttataagtt gtaatgatgt tcatgaaact tgtgactaat
     2401 gctaatatat aaaagagcat atttaaattt agtttctgtt cgtgatagtt ataaactaat
     2461 cattatataa aggagatagt catatgaatg atttaacgca ttttaacaag cgttcaccga
     2521 tatttattat tattttagga tctttgactg ccattggagc attatcgata gatatgtttt
     2581 tgcctggatt accagatatt agacatgatt ttcaaacaac gacgtctaac gcacaattaa
     2641 cattatcgat gtttatgatt ggtttagcgt ttggtaattt gtttgctggt ccaatttcag
     2701 attcaactgg gcgcagaaag ccgcttatta tagctatgat tatttttaca ttagcaagtt
     2761 taggtattgt ttttgtacat aatatttggc tgatggttgc attaagattt cttcaaggag
     2821 tgacaggtgg cgcagcagca gttatttcaa gagatatagc aagtgatatg tatagtggta
     2881 atgaactaac taagtttatg gcattattga tgcttgttaa tggtattgca ccagttgttg
     2941 cgccaacaat agggggcata attttaaatt attctgtctg gcgtatggtc tttgtcattc
     3001 tgactatctt tggcttcgtc atggtcatag gttctttatt aaaagtacct gaatcattaa
     3061 cagtgacaaa tcgtgagtca agtagtggat taaagacaat gtttaaaaac ttcaaaatat
     3121 tattgaagac gccacgtttt gtattaccaa tgctcattca aggtatgaca tttgtaatat
     3181 tatttactta tatttctgcg tctccgttta ttattcaaaa aatttatggc atgactgcaa
     3241 tacaatttag ttggatgttt gcaggaattg gtattacgct cattatatca agtcaactta
     3301 ctgggtacct tgttgatttt atagactcac aaaaattgat gagaggcatg acgatgattc
     3361 agattatcgg tgtaatttta gtgacgattg tattgcttaa tcattggaac ttttggattt
     3421 tagcgatagg atttatcatt ttaatagcac cagtgacagg tgttgcgaca cttggtttta
     3481 caatagctat ggatgaaagt agtagtggta gagggagttc atcaagttta ttaggtttag
     3541 tgcagttttt atttggtggt gttgcatcgc cattagtggg ggtaaaaggt gaagataatc
     3601 ctatacctta cataattatt attatagcta cagcagttat attaattatt t
//
LOCUS       DBNENW010000191         3550 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNENW010000191 DBNENW010000000
VERSION     DBNENW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.195"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            89..1558
                     /locus_tag="AC1LW8_002514"
     CDS             89..1558
                     /locus_tag="AC1LW8_002514"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIP4774273.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            1551..2372
                     /gene="nadE"
                     /locus_tag="AC1LW8_002515"
     CDS             1551..2372
                     /gene="nadE"
                     /locus_tag="AC1LW8_002515"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HIP4774274.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            2643..3245
                     /locus_tag="AC1LW8_002516"
     CDS             2643..3245
                     /locus_tag="AC1LW8_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIP4774275.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            3226..3399
                     /locus_tag="AC1LW8_002517"
     CDS             3226..3399
                     /locus_tag="AC1LW8_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HIP4774276.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
BASE COUNT         1291 a          465 c          698 g         1096 t
ORIGIN      
        1 gtatggaaat taaggagctc ttatgtatat aaaaaaatga attatgataa aatgtaagaa
       61 aatatttagg tcgattggag agatacaagt gtaccaatta gaagacgaca gtttaatgtt
      121 acataatgac ttatatcaaa taaatatggc tgaaagttat tggaatgata atattcatga
      181 aaaaatggct gtatttgatt tgtattttag aaaaatgcca tttaatagtg gctatgctgt
      241 ttttaatggt ttaaaacgcg tcattgattt tatagaacat tttggatttt cagaatctga
      301 tttggaatat ttaaagtcta ttggctacaa ggatgatttc ttatcatatt taaaagattt
      361 aaaattcaca ggaagcattc gttcgatgca agaaggcgag ttatgctttg gtaacgaacc
      421 attgttacgc gtagaagcac cattgattca agcgcaatta atagaaacaa ttttattaaa
      481 cattgtaaat ttccatacac taattacaac aaaggctagc agaattcgtc aaattgcatc
      541 aaatgataaa ttaatggagt ttggtacacg tcgtgcgcaa gaaattgatg cagcattgtg
      601 gggcgctaga gctgcttaca tcgggggctt tgattctaca agtaatgtta gggcggggaa
      661 attatttggt atacctgtgt ctggtacaca tgcacatgca tttgtccaaa cttatggaga
      721 cgaatatgtt gccttcaaaa aatatgctga aagacataaa aattgtgtgt tcctagtaga
      781 tacattccat actttaaaat ctggcgtgcc aaacgcaata aaagttgcaa aagaattagg
      841 tgacaaaatt aactttgtag gtattcgatt agattctgga gatatcgctt atttatctaa
      901 agaggcaaga cgtatgcttg atgaagcagg atttactgaa actaaaatta tcgcgtctaa
      961 tgatttggat gaagaaacga ttacgagttt gaaagcacaa ggtgcaaaag tagattcttg
     1021 gggcgttggt acaaagctga ttacaggata cgatcaacca gcattaggtg cagtatataa
     1081 acttgtagct attgaaaatg aagatggttc atatagtgat cgtattaaat tatcaaataa
     1141 cgctgaaaag gttacgacgc caggtaagaa aaatgtatat cgcattataa acaagaaaac
     1201 aggtaaggca gaaggcgatt atattacttt ggaaaatgaa aatccatacg atgaacaacc
     1261 tttaaaatta ttccatccag tgcatactta taaaatgaaa tttataaaat ctttcgaagc
     1321 cattgatttg catcataata tttatgaaaa tggtaaatta gtatatcaaa tgccaacaga
     1381 agatgaatca cgtgaatatt tagcactagg attacaatct atttgggatg aaaataagcg
     1441 tttcctgaat ccacaagaat atccagtcga tttaagcaag gcatgttggg ataataaaca
     1501 taaacgtatt tttgaagttg cggaacacgt taaggagatg gaagaagata atgagtaaat
     1561 tacaagacgt tattgtacaa gaaatgaaag tgaaaaagcg tatcgatagt gctgaagaaa
     1621 ttatggaatt aaagcaattt ataaaaaatt atgtacaatc acattcattt ataaaatctt
     1681 tagtgttagg tatttcagga ggacaggatt ctacattagt tggaaaacta gtacaaatgt
     1741 ctgttaacga attacgtgaa gaaggcattg attgtacgtt tattgcagtt aaattacctt
     1801 atggagttca aaaagatgct gatgaagttg accaagcttt gcgattcatt gaaccagatg
     1861 aaatagtaac agtcaatatt aagcctgcag ttgatcaaag tgtgcaatca ttaaaagaag
     1921 ccggtattgt tcttacagat ttccaaaaag gaaatgaaaa agcgcgtgaa cgtatgaaag
     1981 tacaattttc aattgcttca aaccgacaag gtatcgtagt tggcactgat cattctgcag
     2041 aaaatataac tggtttttat acgaagtacg gtgatggtgc tgcagatatc gcacctatat
     2101 ttggtttgaa taaacgacaa ggtcgtcaat tattagcgta tcttggtgcg ccaaaggaat
     2161 tatatgaaaa aacgccaact gctgatttag aagatgataa accacagctt ccagatgaag
     2221 atgcattagg tgtaacttat gaggcgattg ataattattt agaaggtaag ccagttacgc
     2281 cagaagaaca aaaagtaatt gaaaatcatt atatacgaaa tgcacacaaa cgtgaacttg
     2341 catatacaag atacacgtgg ccaaaatcct aatttaattt tttcttctaa cgtgtgactt
     2401 aaattaaata tgagttagaa ttaataacat taaaccacat tcagctagac tacttcagtg
     2461 tataaattga aagtgtatga actaaagtaa gtatgttcat ttgagaataa atttttattt
     2521 atgacaaatt cgctatttat ttatgagagt tttcgtacta tattatatta atatgcattc
     2581 attaaggtta gtttgaagca gtttggtatt taaagtgtaa ttgaaagaga gtggggcgcc
     2641 ttatgtcatt cgtaacagaa aatccatggt taatggtact aactatattt atcattaacg
     2701 tttgttatgt aacgttttta acgatgcgaa caattttaac gttgaaaggt tatcgttata
     2761 ttgctgcatc agttagtttt ttagaagtat tagtttatat cgttggttta ggtttggtta
     2821 tgtctaattt agaccatatt caaaatatta ttgcctacgc atttggtttt tcaataggta
     2881 tcattgttgg tatgaaaata gaagaaaaac tggcattagg ttatacagtt gtaaatgtaa
     2941 cttcagcaga atatgagtta gatttaccga atgaacttcg aaatttagga tatggcgtta
     3001 cgcactatgc tgcgtttggt agagatggta gtcgtatggt gatgcaaatt ttaacaccaa
     3061 gaaaatatga acgtaaattg atggatacga taaaaaattt agatccgaaa gcatttatca
     3121 ttgcgtatga acctcgaaac atacatggtg gattctggac taaaggcatt cgtcgtagaa
     3181 agcttaaaga ttatgaacca gaagaactgg aaagtgtagt agaacatgaa attcaaagta
     3241 aatgagaatg aaacaattgc tgattgtttg tcacgaatga aaatgcaagg gtatatgccg
     3301 gtaaaacgta ttgaaaaacc cgtgtttcaa gagcaaaaag atggcacggt tgaagtatca
     3361 catcaagaaa tcatttttgt aggtaagaaa atccaataac ataatccaat ttaaataaag
     3421 actatttgaa gaggaaaggc tattcaaagt ttgagtaatt ttactttgaa tagcctattt
     3481 gtttatacat gcaagatgct cgatccatat tgtatgagaa acccccagca agctatataa
     3541 agcatatgct
//
LOCUS       DBNENW010000192         3540 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNENW010000192 DBNENW010000000
VERSION     DBNENW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.196"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(399..3446)
                     /gene="fnbA"
                     /locus_tag="AC1LW8_002518"
     CDS             complement(399..3446)
                     /gene="fnbA"
                     /locus_tag="AC1LW8_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HIP4774277.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATENKVNETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANLETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVVEAKEASDEKVETGTDVTSKVTVESGSIEAPQGNKVEP
                     HAGQRVVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEI
                     LGNGNIRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPV
                     VYNPGVSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAG
                     GQPTVKVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLD
                     KTYVIHYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADG
                     NGKNGQIIQNNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGG
                     YVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIQGQNNGNQSFEEDTEK
                     DKPKYEQGGNIIDIDFDSVPQIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNNKA"
     gene            complement(3430..3532)
                     /locus_tag="AC1LW8_002519"
                     /pseudo
     CDS             complement(3430..3532)
                     /locus_tag="AC1LW8_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          855 a          704 c          548 g         1433 t
ORIGIN      
        1 aataatcgcc tgtacaatgc atcattaaca agtcactgaa acgcctttca ttgtattaat
       61 aacgtcacta caatttttat atcgttcggt ttttgtttgg ttttaatgat tgtttataca
      121 gaaacagccg tatttcaaga cgatatttta aatttaacta aatttgcatc tagttaataa
      181 ttgcatttat ctaatttgtc ttattgattc aatctaattt gtacttacaa actagtttta
      241 aattctaact ttatctctca gttcgttatc aatcatcaga cataaaccaa tgaagcaatc
      301 agaaaacact ctaattttct attagaaatt tgatttaatc taaaaaaaca ggtttacttc
      361 atataattta tgaaataaac ccgtcaattt ttgtttaatt atgctttgtt attcttttta
      421 tttctgcgta ataatgctaa acctagaatg ctgaataatc cgccgaacaa catacctttg
      481 tttgttgatt cttctccacc tgtttcaggt agttcagatt tcttagattg tgctttttta
      541 gttggtgcca ctgctttaac cttttcattg atttcaataa caggtgttac tactttacct
      601 tgttccactg gtttagaagg ctttttaggt tcttcttttg cgggtggtac tggtttacca
      661 ggttcagctg gtacctctgg tgttggcggt gttggtgttt ctggctcact cggcacttct
      721 ggtgtcggtg gtgttggtgt ttccggctca cttggtactt ccggagtcgg tggtgttggt
      781 gtttccggct cacttggtac ttctggtgtc ggtggtgttg gcggcgttgt atcttcttca
      841 atcgtttgtt gaccttcatt ttgaccgctt acttttggaa gtgtatcttc ttcaaaatca
      901 acactgttgt gtccaccgaa ttgataattt ggtttatctt tgtttgtatc ttcttcaata
      961 atttcagtgt gcttattgaa tccatgaatt tgtggcacac tgtcgaagtc gatatcaatg
     1021 atattaccac cttgttcata cttaggtttg tctttctctg tatcttcttc gaatgactgg
     1081 ttaccattat tttgaccttg gatttgaggt acactgtcga aatcgatatc tacgatattg
     1141 ccaccttgtt catatttagg tttgtcttct tctgtgtctt cctcgaatga ctggttaccg
     1201 ctattttggc caccttcata acctaattca ctcttaatat caacatgact attttcttcg
     1261 atttcttcaa tcacgccata attaccgtga ccattttcag ttcctaaacc agaatgagaa
     1321 atatgatgat tgttttcagt gatttcctcg attggacctt gagcttgacc atgttcttct
     1381 ggtagttcat ccactagttc aatcagatta ctttcagtcg tatattcttt cgtatcttca
     1441 attgttgtat gatcgctcac tgcgccagtt acaatacctt ttgtagactc ttcgtcaaat
     1501 tcaactaagt tagactcagt agtaacctga ccaccacctg gatttgtatc ctcttcatat
     1561 tcaacaacat cagcgtgatg ttttgaattt tcatgtgtcg attcttcaaa gtcaattgga
     1621 tttgattcct cagaggactc agtgtatcct ccaacgtgac ccgcttcact atccacagca
     1681 gtatggtaat cgatatcaat agctgatgaa tccgtttctt ctattgtttc aatataccca
     1741 tcaacataac caccctcacc atctatagct gtgtggtaat caatgtcaag cggtgtattg
     1801 tcttgatttt cttctgtttc aataatttgc ttggcatcgt actgcccgct catagttcct
     1861 tttgcagtat cttctttata ttcaaaatca ttattttgaa taatttgtcc atttttacca
     1921 ttgccgtcag ctttattgct atataaaact aaaccattat cccaagttaa acgatatccc
     1981 ccataaacat agtaagattt atatgctcgt tctggatacc catataattc agttctaaaa
     2041 ttaacctgat ctgacccttg caaatattca cctgtataat gaatgacata cgttttatcc
     2101 aacttatcta aattcaatga gtaactacca ttgtcttgca cactcaattt tccattcatt
     2161 tcctttgtta catctttgaa tttgttagta tctgatgtat ttgcataaac actttgtggc
     2221 aattcatctt ttttccctag atattcatat actttaacag taggttgtcc accagctaaa
     2281 ttactacctt cagtcaacgt ccctgttact gatacagtgt ttgactggtt tccattcatt
     2341 ggcttaatat aagctatatg tgtaaattta ttagattctt tattaaatgt ttcaattgat
     2401 ccatttacat ttgtataact attgctaaca cctggattat aaacaactgg tattgttttt
     2461 tctgtttctt caccatttaa tttagaagta atcttttgtt ctccattgct ttgtacagtt
     2521 ttagggtcaa taaataagtt gatttctaaa ttagctgtta cctctacctt gtgttcaatt
     2581 tcgttagtaa atgtatatct tatgttacca ttccctaaga tctcacctgt agccattaca
     2641 actgagccat ttttaatctc tggtaccttt ctagctgttg aaaccccata agtatttaca
     2701 ttatttgata atgtaaaatc aaaataatct cctcttttta atccatctgc gaatttcaat
     2761 ttgtatttca atacgacacg ttgaccagca tgtggctcta ctttatttcc ttgaggtgcc
     2821 tcaatagaac cactttctac tgtaacttta cttgtcacat ctgtacccgt ttccactttc
     2881 tcgtcactag cttccttagc ttcaactacg tctgctgatc ttgtcacacg tggtttactt
     2941 tctgatgccg ttcttggctg tgccacttca acttgtgttt ctgcgacttg attttgtgtg
     3001 gcctttttag gtgttaaatc tacttgtctt tgatctccgc tattgtcttg agattgtgtt
     3061 gtttccttaa cttgaggttt cgcttcttcc ttaactacct cttctttaac tgtttctaaa
     3121 tttgctggtt gtgcagtttg tggtgcttgt actgcttttg gtgcttcttc agttgttact
     3181 tgtgttgcgt ttgacggttg ttctgttgct gttgcgctgt atgattgtgt ttcatctata
     3241 gtattaacgt tagttgtagt tgtttgtgtt tcgtttactt tattttcagt agctgaattc
     3301 ccattttctt ctactgtagt tgtcttttgt tctgatgctg cagcttcttt atcttgtccc
     3361 atcccaacaa cgatcattgt tcctaagaat actgatgctg ctcccaattt atgttttcta
     3421 atgccgtacc taagattgtt tttcactata atatctccct ttaaatgcaa aattcattaa
     3481 tttttttaaa cttaataaat gcaagtctat attgttcggt ttttaaaagc aatacaaaaa
//
LOCUS       DBNENW010000193         3536 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNENW010000193 DBNENW010000000
VERSION     DBNENW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.197"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(257..1072)
                     /locus_tag="AC1LW8_002520"
     CDS             complement(257..1072)
                     /locus_tag="AC1LW8_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774278.1"
                     /translation="MIHSKRLKLCLCLIILSVFIGACGMKKEESSKDKQIKENFNKIL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMIIETNNSNMESRGMVLYINRNT
                     RTTKGNFVVREITEDSKGYSHSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYNIPTNKAPKLLLK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTPSEDTSYESNGISNKSW"
     gene            complement(1186..1981)
                     /locus_tag="AC1LW8_002521"
                     /pseudo
     CDS             complement(1186..1981)
                     /locus_tag="AC1LW8_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            complement(2093..2851)
                     /locus_tag="AC1LW8_002522"
     CDS             complement(2093..2851)
                     /locus_tag="AC1LW8_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774279.1"
                     /translation="MIHSKRLRLWLYLVLLAVFISACGMKEDKQIKENFNKTLSLYPT
                     KNLDDFYDKEGFRDQEFEKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRNTRTTKG
                     NFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFVQYGDF
                     KDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYDIPTKKAPKLLIKGDGDL
                     KGSSIGHKNLEFTFVENKEENIYFTDSINFKPTK"
     gene            complement(3157..>3536)
                     /locus_tag="AC1LW8_002523"
     CDS             complement(3157..>3536)
                     /locus_tag="AC1LW8_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HIP4774280.1"
                     /translation="MGYMIRSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKS
                     NRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGS
                     NVVTMDLALDKAVRDDIYRYITN"
BASE COUNT         1029 a          628 c          401 g         1478 t
ORIGIN      
        1 ttgcggtgct tatattaata atggcatgaa tgaacgcatg ctgtcttagt ttcaggaatc
       61 ggctagaata ttaaacttcc gttctttgaa tattttttaa tttcttacta atatcatcca
      121 gttcattctt cgccttttta ccccaatctc cttgagctac attatcaaat tcacctttaa
      181 attttttgaa atggctacct atatttcaca gctcacttta gatatcgaac ttgtttcttc
      241 agagttactt gttatattac caggatttat ttgatattcc gtttgactca taacttgtat
      301 cctcacttgg agtatattgt acagaatctg taaaataaat gttttcttct ttattttcta
      361 caaaggtaaa ttctagattt tttgacccta cagatgagcc ttttaaatca ccatcacctt
      421 tcaataatag tttcggagct ttgttggttg gaatattata tctttttcga agttgcttca
      481 cattatagtc atcattactt aattgatact ttgctgaata acttggcaca ttcggattat
      541 atgaaatgtc accatcttta taatcattaa tatctttaaa gtcaccgtat tgtacaaaga
      601 atttaaagtt ttcgatttct tttcttaatt tatcatcagc tatcggtttg gttggaatga
      661 ttctattatg ttccattttc actggatatt ttgtatcttt actatgtgaa taccctttac
      721 tatcctcggt tatttctcta acaacaaaat tacctttcgt tgttctagta ttacgattta
      781 tatagagcac cattcctcta gattccatat tgctattatt tgtctcaata atcattttag
      841 agtgaattat ccaagtgcct ttgtcacctt tttcaaactc ttcgtcacga aagccttcct
      901 tatcatagaa gtcttcaaga tttttggttg gatataaact caatattttg ttgaaatttt
      961 ctttaatttg tttatcttta ctactttctt cttttttcat tccgcaagcg cctataaaaa
     1021 cagatagtat tatcaggcat agacacaatt ttaacctttt tgaatgaatc atcatttctc
     1081 caccttatat ttttaatact gactcaatct ttaatttata catttaaagc gaaaaatttt
     1141 ttaacacttt atgaaatttt caatccaaac ttctattaac cctatttagt ttgactcata
     1201 acttgtatcc tcgcttggag taaattcaac cgaatctgta aaatagatat tctctctttt
     1261 attttgtaca aaggtaaatt ctaagtcttt agagcctatg gatgaacctt ttaaatcgcc
     1321 atcacctttc aacaatagtt ctggtgcttt tttagtcggt atatcatatc tttttcttaa
     1381 ttgttggata ttataatcat cattacttaa ttgatatttt gccgaataac ttggtacgtt
     1441 tggattgtat gaaatatccc catttttata atctttaaaa tctttaaagt tgccatattg
     1501 tacaaaaaaa cttgaagttt tcaatttctt tttttaactt ctcgtctttt ataggttttg
     1561 ttggtatgat tttattatgt tccatcttga cagggtattt tttatcttta tcatgtgtat
     1621 atcctttttt atctttagtt atctcaccta ctataaaata tcctttcgtt gttctagtgt
     1681 tacgattgat gtaaagtacc atctctctgg attttaaggc tcctcctttt aattggatgt
     1741 tcattccaga attaactatc caagtacctt tatcaccctt tttaaattct tgatctcgaa
     1801 agccttcttt atcgtaaaaa tcatcgagat ttttggttgg atataatttt aatgtttgat
     1861 taaatgtgtc tttaatttgg gcgttttgac catttgcttt tgtcataatg acataaccta
     1921 caatcaatat aatgagtaat accaagcata taccgagcgt taatttcttt gaatgcatca
     1981 tcatttctcc accttatatt tttaatactg actcaatctt taatttacac aactaaaacg
     2041 aaaaatcatt ttgaacactt tatgaaattt tcattccaaa cttctattaa ccctatttag
     2101 taggtttgaa attaatacta tccgtaaaat agatgttttc ttctttattt tctacaaagg
     2161 taaactctaa atttttgtgt cctatagatg atccttttaa atcaccatcg ccttttatta
     2221 ataatttagg tgctttctta gttggtatat catacctctt tctaagttgc ttcacattgt
     2281 agtcattgtt actcaattga tattccgccg aataactagg tacattggga ttatatgata
     2341 tgtcaccatc tttataatca ttaatatctt taaagtcacc gtattgtaca aaaaacttaa
     2401 agttttcgat ttcttttctt aatttatcat cagctatcgg tttggttggg ataattcgat
     2461 tatgttccat tttcactgga tattttgtat ctttactttg tgcataccct ttactatctt
     2521 cccacaattc tctaacaata aaattacctt tcgttgttct agtattacga tttatataga
     2581 gcaccatact tctagattcc atttttttat ccttcagctc aactaccatc tcagaatcaa
     2641 caatccaagt ccctttatct cctttttcaa actcttgatc tcgaaatcct tctttatcat
     2701 aaaaatcatc gagattcttg gttggatata aactcaatgt tttgttgaaa ttttctttaa
     2761 tttgtttatc ttctttcata ccacacgcgc taataaaaac agctaataat actaaataca
     2821 gccagagcct caaccttttt gaatgaatca tcatttcaca cccccatttt tatttttgat
     2881 gcaaagttaa gttcaaattc caatattaat aatactagag ggaattttat gattaaatcc
     2941 tcttggcttt taattgttag ttgtattttt cacatatcaa catcacaaat tatttaaaaa
     3001 gcgcaaatat ctttataatt tttattggcc taaccaacta attaattaag ataaattgcg
     3061 cttatattta aaataataac actgaaactc aatgtcttta cttattaaaa ttgatgttta
     3121 aatacatctt tactaacatt catttttcgg tttacattaa tttgttatat agcgatatat
     3181 atcatctctt acagctttat cgagtgctaa atccatcgtt actacgtttg aaccattcgg
     3241 cattttatct tgcttctctg aaccttctat atatccagca gttcctaaat aataaaaata
     3301 tataccatca tcatcttttt tctgtacaaa aatatacatt ttaattcctt ttgctctatg
     3361 ggacaaaatt ttctgaactt cttttgattt tagcgttcta ttagatttag taaaccattt
     3421 taattcatct tgacttaaaa attcatcttc atattttgtg ctatcactta tatcctcatg
     3481 cttatcataa gtgataaata ttggcatttc ttggcttctt atcatgtatc ccatga
//
LOCUS       DBNENW010000194         3492 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.198, whole genome shotgun sequence.
ACCESSION   DBNENW010000194 DBNENW010000000
VERSION     DBNENW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.198"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            38..1420
                     /gene="spa"
                     /locus_tag="AC1LW8_002524"
     CDS             38..1420
                     /gene="spa"
                     /locus_tag="AC1LW8_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HIP4774281.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNKKPGKEDGNKPGKEDNNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAAD
                     NKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGA
                     ALLAGRRREL"
     gene            complement(1749..3341)
                     /locus_tag="AC1LW8_002525"
     CDS             complement(1749..3341)
                     /locus_tag="AC1LW8_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIP4774282.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPVRVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFVIDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLKESEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1309 a          677 c          533 g          973 t
ORIGIN      
        1 tatgatgact ttacaaatac atacaggggg tattaatttg aaaaagaaaa acatttattc
       61 aattcgtaaa ctaggtgtag gtattgcatc tgtaacttta ggtacattac ttatatctgg
      121 tggcgtaaca cctgctgcaa atgctgcgca acacgatgaa gctcaacaaa atgcttttta
      181 tcaagtgtta aatatgccta acttaaacgc tgatcaacgt aatggtttta tccaaagcct
      241 taaagatgat ccaagccaaa gtgctaacgt tttaggtgaa gctcaaaaac ttaatgactc
      301 tcaagctcca aaagctgatg cgcaacaaaa taacttcaac aaagatcaac aaagcgcctt
      361 ctatgaaatc ttgaacatgc ctaacttaaa cgaagcgcaa cgcaatggtt tcattcaaag
      421 tcttaaagac gatccaagcc aaagcactaa cgttttaggt gaagctaaaa aattaaacga
      481 atctcaagca ccgaaagctg acaacaattt caacaaagaa caacaaaatg ctttctatga
      541 aatcttgaac atgcctaact tgaacgaaga acaacgcaat ggtttcatcc aaagcttaaa
      601 agatgaccca agtcaaagtg ctaacctatt gtcagaagct aaaaagttaa atgaatctca
      661 agcaccgaaa gcggataaca aattcaacaa agaacaacaa aatgctttct atgaaatctt
      721 acatttacct aacttaaacg aagaacaacg caatggtttc atccaaagct taaaagatga
      781 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatgatg cacaagcacc
      841 aaaagctgac aacaaattca acaaagaaca acaaaatgct ttctatgaaa ttttacattt
      901 acctaactta actgaagaac aacgtaacgg cttcatccaa agccttaaag acgatccttc
      961 agtgagcaaa gaaattttag cagaagctaa aaagctaaac gatgctcaag caccaaaaga
     1021 ggaagacaac aacaaacctg gtaaagaaga caacaaaaaa cctggtaaag aagatggcaa
     1081 caagcctggc aaagaagaca acaacaaacc tggtaaagaa gacggcaacg gagtacatgt
     1141 cgttaaacct ggtgatacag taaatgacat tgcaaaagca aacggcacta ctgctgacaa
     1201 aattgctgca gataacaaat tagctgataa aaacatgatc aaacctggtc aagaacttgt
     1261 tgttgataag aagcaaccag caaaccatgc agatgctaac aaagctcaag cattaccaga
     1321 aactggtgaa gaaaatccat tcattggtac aactgtattt ggtggattat cattagcgtt
     1381 aggtgcagcg ttattagctg gacgtcgtcg cgaactataa aaacaaacaa tacacaacga
     1441 tagatatcat tttatccaaa ccaattttaa cttatatacg ttgattaaca cattcttatt
     1501 tgaaatgata agaatcatct aaatgcacta gcaacatctt ttgttgctca gtgcattttt
     1561 tattttactt acttttctaa acaacttctg aaacgcctca acactttcta ctctaattac
     1621 atatacgaca tttttagaca ttaaaaaatc gaactagaca agatgctcaa tgcatttcgt
     1681 actagttcga ttcatgaata attagattta aaatgtcatt tgaatccaag tgacaacatt
     1741 atttatattt agaatattaa cgttagtata aacgtccaaa cacaaataaa agcaacaaat
     1801 ataatactgt attttagcgt catttttaat aatgcagatt cttcaccaac ttttttaaca
     1861 gctgcagtcg caatggcaat tgattgtggt gaaataagtt tcgctgctac accacctgca
     1921 gtgttagctg ccacaagtaa tgaaccgctt gttgaaattt gttgtgctac tgtcgcttga
     1981 ataggtgcga ataaagtatt gttatttaca actgaaccag tcataaacac accaatccaa
     2041 cctaatactg gagagaataa tgggaaaatt gctccagctt tagcaatacc ttgtccaatt
     2101 gctacagtca aaccaccgta tgtcataact ttagcaatag ctaggatagc tgaaattgta
     2161 aggatcggta accataattc tttaattgct tcgaccaata aagcacctgc acttttccat
     2221 tttagcttcg taattaaaat tgtaataatt actgttaata aaatcgctgt cccagttgca
     2281 ccaattaaat cgagacgcaa cgcaattcct ttaggcgata aatcactcac agtatttgga
     2341 attggcaatt ttattactaa actttcaagt gcacctccag gttggaataa ttttttgaag
     2401 aatggtgcac tccatactaa aacaaaggca gttaaaatga cgaacggact ccaagcaaag
     2461 acaatttctt taggcgttcg tttttgaatt ttatgttctg attctttcaa cctgaaaatg
     2521 tttttcggtt taaatttacg acaaacaaat gctaacacca ccattgttgc tagtgatggg
     2581 ataatgtctg ctagttctgg accatggaat attgttaata ataattgtaa tccagtatat
     2641 gtaccactca ctgttaaaat gacaggtaaa atttctttaa tacctttcat gccatctata
     2701 acgaatacta aaacaaatgg gataataaag tttaaaattg gaagtgttaa tgctgaatat
     2761 ctcgcaacat ctaatgttgt aacgcctcca cttaagttaa acgtatcaat aatactaact
     2821 ggtaaaccaa ttgcaccaaa ggcacccgcc gcaccattag caattaagca taacatcgct
     2881 gcttttaatg gttcaaatcc aagttgaatt aataatactg cacaaatcgc aattggcaca
     2941 ccaaatcctg ctgcaccttc taaaaatgcg ttgaaacaaa atccaattaa taatagttgg
     3001 attctttggt cctctgaaat acttgcaata ctatcttgaa taatagaaaa ttgtcctgtt
     3061 ttaatagaaa ctttatataa ccaaactgcc attaaaacga tatatcctat tgggaaaata
     3121 ccggcaacaa cgccttctgt aatcgcacct gctgatacac gcactggtaa ttcaaataca
     3181 aataaagcca caatcaatgt aacaaccaaa gttgtcaatg ctgcataaat gcctttcatt
     3241 ttaaaaacgg ttaagcataa taaaaataaa ataataggta ctgctgcaac taaggctgat
     3301 aatccaacat tatcgaatgg atttacagta agtagtgtca taatgactcc ctctctttat
     3361 ataaaatatt tatcatcctg attaatctac aacctatttc aacttatatt ttgcgatgat
     3421 cacatattta aaatgtaaca ctcctatatg tgacaggcaa tcgaattttt acaaaaagtt
     3481 cacaaaatat ac
//
LOCUS       DBNENW010000195         3490 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNENW010000195 DBNENW010000000
VERSION     DBNENW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.199"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(47..1291)
                     /gene="fabF"
                     /locus_tag="AC1LW8_002526"
     CDS             complement(47..1291)
                     /gene="fabF"
                     /locus_tag="AC1LW8_002526"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIP4774283.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1303..2244)
                     /locus_tag="AC1LW8_002527"
     CDS             complement(1303..2244)
                     /locus_tag="AC1LW8_002527"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIP4774284.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2539..2724
                     /locus_tag="AC1LW8_002528"
     CDS             2539..2724
                     /locus_tag="AC1LW8_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HIP4774285.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(2981..3415)
                     /locus_tag="AC1LW8_002529"
     CDS             complement(2981..3415)
                     /locus_tag="AC1LW8_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HIP4774286.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1065 a          685 c          508 g         1232 t
ORIGIN      
        1 aaaaaatctt cagtaactaa aacaacgtta ctgaagattt ttataattat gcttcaaatt
       61 tcttgaatac taatactgcg ttatgtccac cgaatcctaa gctattactc attgcataag
      121 taatatcaag gtcttgcgct tcatttggaa caatatccaa atcacattct ggatctggtg
      181 ttaccgcatg aattgtcggt gcgactttag agtctttaat tgaaagcgct gagaagattg
      241 cttcaattcc acctgttgca ccaagtaagt gaccagtcat tgattttgtt gagctaactt
      301 ttaagtgttt agctgcttca ccaaatgtat ttttaatagc tttaacttca tttaagtcac
      361 caacaggagt acttgtacca tgggcattta agtattgtac atctttaggt tcaataccag
      421 catcatccat agctgcttgc attgctctag aaccgccttc accttctgga gctggcgctg
      481 taatatgata agcatcacct gtagtaccat agccaactat ctcagcataa atattggcac
      541 ctcgagcttg tgctgattct aaagattcaa ttactaaaat accagcacct tcacccataa
      601 caaaaccatc tctaccttct tggaatggac gacatgctgt ttcaatgtca tcatttgtag
      661 aaagcgctcg acttgcactg aaaccagcaa ttgccatatg agtaattggt gcttctgtac
      721 caccagtaat cattgcatct gcatcaccgc gttgcacaat tttaaatgct tctccgattg
      781 aatttgtacc tgttgcacat gctgtaactg ttgcaccatt tggtcctttt gcacctaagt
      841 caattgatac ttgcccagtt gccatatcag gaattaacat tggtacgaaa aatggactca
      901 cacgtcttgg gcctttatcc attaattgtt tatgtgcaat ttcaaatgtt tccataccac
      961 cgataccaga accaatccat acaccgattc gatctgcagt attttcattg atatctaatt
     1021 gcgcatcttt aacagcctct ctagctgcta caattgcata ttgagtaaat ctatccatac
     1081 gacgcgcttc ttttttgtcg atatgatctt caatattaaa gtttttaagt tctcctgcta
     1141 agtgaacgct ataaggttca gtatcgatac gtgtaatttt atcgatacca tttacgcctt
     1201 ttagagcatt ctcccatgtt gttttgacat cattaccgat tggagaaagg gctcccatac
     1261 ctgtaataac tactctttta ttttgactca ttcgttatcc tcctattttc cccattttat
     1321 tgtcattgcg ccccaagtta ggccgccacc gaatccgaca agaacaattg tatcatcatc
     1381 tttgatttta ccatttttta attcttgatc gatacttaaa ggtattgacg cagctgaagt
     1441 atttccatat ttatttacag aaacactcat tttgtctttt gaaataccta agcgttctct
     1501 agctgattcc ataattctaa tattagcttg atgaggaata aataaatcta tatcatctga
     1561 tgttaaattc gctttttcaa ctacacgtgt tgatgcatca cccataattc taacagcaaa
     1621 tttaaatact tctcgaccat tcattttcag tttaccagta tctttatcta aatataaatg
     1681 tttaccacct gtgccatcag aacccatttc ataacttata atacctctgc catctgaaac
     1741 ttcaccgatg ataaccgcac ctgcaccatc tccaaataga actgcagtag aacggtcagt
     1801 taaatctgtt attttagata atttatctgc accgacaact aaaatgttat gataatctcc
     1861 agattgaaca tattgtttag ctgtaatcat tgaatacata aatccagaac atgctgcaag
     1921 ttgatccata gaggcaactt tgcccgtccc taaacgttct tgcaacatat ttgcgacagt
     1981 tggaaatggc atatctccag ttgctgtggc aacaattatc atatctatat cttcgggctg
     2041 aataccagcg tcagcgattg cttttaaact tgcttcatat gctaaatctg aagtatcttg
     2101 atcatcatct gcccaatgtc tttctttaat tccagtcatc ttagaaatcc attcatcaga
     2161 tgtatctaaa aattgctcaa aataggcatt gtcaataatc ttttctggcg catatgcacc
     2221 aaaaccttta atacccacgt tcatagttga acaccttctt aataaaattt ttaataccag
     2281 gtattaattt aacatagatt actttttaaa ctcaagtcag aggtaggaat attgactagt
     2341 taataaaatt ttaatgcttt attatcgtca aatttcagta tgaaattggt tttaatgcgc
     2401 ttaatttaga atagccattc agataaaatc atttatttaa actgtataat atggtcaaaa
     2461 tatatgagat gttactatac aaatttctaa ctttcactta aaataaaata tatataatta
     2521 aagtggaggt agaatgacat gaaacacctc gtaacattct tttgggcatt attattaatg
     2581 caaatggtta actttgtact taatagccta gtaggtggag acagcttaaa tgtagtgaat
     2641 ccaattatca tggcagtatt gttcacgatt ttcacagcaa tttttgctgc agtaattaaa
     2701 ccgccgaaag attcatcaca ataactattt agtataaaat gaaatatgtc tttcttatta
     2761 ttgaagctgc ccagataaag gcggctttta tttttggcca ttaatgtttt gtattttgaa
     2821 tattttaaag ctcaatgacc tctaacccat cacttttaaa tttagaaaaa gccttaatac
     2881 aaagatgtca tttacgagta atctttgtat caaggcaatt attttataat catcactgta
     2941 aaattaaatg cttcatgttg aatttgataa ttttaagtgt ttatttagtt tttacattaa
     3001 tatcaatttg tttaattgaa tttgaatcaa acaagtttgc tgtgtagcta tctttcttaa
     3061 gatccacttc ttttttacta ccatctttcc aagtaatcgt gtaaacggca gattttgcat
     3121 ttcgaatctt ctcaggtgtt acaccgcgtt catcatataa caaagcttta actttatttc
     3181 ctaaatctaa atatgataat tggctatctt ttggtaaatt taagtaagat tgattaaaag
     3241 ccataatgcc atctactgca attgtatatg gaacattttg catttcttta gctttttgag
     3301 ttgccggtag tttgtccttt tctgctgcag atacctcatt acctgccaca gtagcgccag
     3361 tcgttgcgat catgcccagt gccaaagtta cagttataaa tgattttaat ttcatataat
     3421 ctctctcctt tgtgagttaa agtatcttac aaaatcaata taacacaaat tgaaaagttt
     3481 caaaaattat
//
LOCUS       DBNENW010000196         3465 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNENW010000196 DBNENW010000000
VERSION     DBNENW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.200"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            135..1364
                     /locus_tag="AC1LW8_002530"
     CDS             135..1364
                     /locus_tag="AC1LW8_002530"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIP4774287.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1559..2416
                     /gene="accD"
                     /locus_tag="AC1LW8_002531"
     CDS             1559..2416
                     /gene="accD"
                     /locus_tag="AC1LW8_002531"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIP4774288.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNNMRQTLSEI
                     LKIHQEVTK"
     gene            2416..3360
                     /locus_tag="AC1LW8_002532"
     CDS             2416..3360
                     /locus_tag="AC1LW8_002532"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIP4774289.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
BASE COUNT         1233 a          471 c          695 g         1066 t
ORIGIN      
        1 cgctatttgt gttacataat caaaaccata aattcttact cattcagatt tacccaatat
       61 ttttactttt ataatgtaat gcgttttatc caagttattt tttaaaaata aatattgaat
      121 taggggctga tttcatgtca ttaagagatg aagcattgga aatgcacaaa cgtaatcaag
      181 gtaaattaga agttaaacca aatgtaaaag ttactaataa agaggaatta agtttagcat
      241 actcacctgg cgttgctgaa ccgtgtaaag atatttatga agataaaaga aaagtatatg
      301 attacacaat taaaggaaat acagttgcag ttattactga tggaacagcg gtattaggtt
      361 taggtaacat tggacctgaa gcaagtattc ctgtaatgga aggtaaagca gtattattca
      421 aaagcttcgc tggtatcaat ggggtgccta ttgcgttaaa tacaactgat accgaagaaa
      481 tcattaaaac agttaagttg ttagaaccta attatggtgg tattaattta gaggatattt
      541 cggcaccacg ttgttttgaa attgaagaac gattgaaaaa agaaactaat attccggtat
      601 tccatgacga tcaacatggt acagcaattg taacaatggc aggtttggta aatgcattga
      661 gagttgttaa caaagatatt gctaaaataa aagttgtact aaatggtgct ggtgcagcag
      721 gaatagccat tgttaaatta ctatacgcgt atggtgtaag aaatatggtt atgtgtgact
      781 caagaggcgc aatttttgaa ggacgttcat atggtatgaa tcctacgaaa gatgttgtag
      841 ctaaatggac aaataaagat aagattgaag ggtctttaga agaagtcgta aaagacgcag
      901 atgtatttat cggagtttct gtagctaatg cgctgtcaca agatatggtt aagagtatgg
      961 cagataatcc aattatattt gcaatggcta atccaaatcc tgaaataata cctgatgatg
     1021 ccaaagcggc aggtgcacga gttgttggta caggacgttc agactatcct aaccaaatta
     1081 ataatgtatt agctttccct ggtattttta gaggtgcatt agaggttgaa gctacacata
     1141 taaatgaaga aatgaaaaag gcagctgtag aagcgattgc tgatttaatc gatagttctg
     1201 aattaaatga agactactgt atcccaggac cgtttgataa acgtgtagcg ccatcagttg
     1261 ctcgtaatgt tgctaaagcg gcaatggaat ctggagtagc taggattgaa gttgatccgc
     1321 aagatgtgta tgataaaaca atgaaactta cagatttaca ataaaaataa ttaacgttta
     1381 attaaattgc ttaaaagtta gcaataaaga gtcattaaaa tgatgaaaat gaaagtttat
     1441 gataaacatt caacagtcaa acgaatataa atcaaataaa tttaaacccg tttttaactg
     1501 gtcaagttca gtttaaggct ctaaatggtt agaacagagg ttatttggag gttttcctat
     1561 gtttaaagat ttttttaatc gaacaaagaa aaagaaatat cttacagtac aagactctaa
     1621 aaataatgat gtgcctgcag gtattatgac taagtgtcca aagtgtaaga aaattatgta
     1681 cacaaaagaa ttagctgaaa atttaaatgt gtgctttaat tgtgatcatc atattgcttt
     1741 aactgcgtat aaacgtatag aagcaatttc tgatgaagga tcatttacag aattcgataa
     1801 gggaatgacc tctgcgaatc cattagattt tccaagttat ttagaaaaaa ttgaaaagga
     1861 ccaacaaaag acaggtctta aagaagcagt tgtgactggt acagcgcaac tagatggtat
     1921 gaaatttggc gttgctgtca tggattcacg ttttagaatg ggaagtatgg gatcggttat
     1981 cggtgaaaag atatgtcgca tcattgatta ctgcactgag aaccgtttac catttattct
     2041 tttctctgca agtggtggtg cacgtatgca agaaggtatt atttccttga tgcaaatggg
     2101 taaaaccagt gtatctttaa aacgtcattc tgacgctgga ctattatata tatcatattt
     2161 aacacatcca actactggtg gtgtatctgc aagttttgca tcagttggtg atataaattt
     2221 aagtgagcca aaagcgttga taggttttgc aggtcgtcga gttattgaac agacaataaa
     2281 cgaaaaattg ccagatgatt tccaaactgc agaattttta ttagagcatg gacaattgga
     2341 taaagttgta catcgtaata atatgcgtca aacattgtct gaaattctaa aaatccatca
     2401 agaggtgact aaataatgtt agattttgaa aaaccacttt ttgaaattcg aaataaaatt
     2461 gaatctttaa aagaatctca agataaaaat gatgtggatt tacaagaaga aattgacatg
     2521 cttgaagcgt cattggaacg agaaactaaa aaaatatata caaatctaaa accatgggat
     2581 cgtgtgcaaa ttgcgcgttt gcaagaaaga cctacgaccc tagattatat tccatatatc
     2641 tttgattcgt ttatggaact acatggtgat cgtaatttta gagatgatcc agcaatgatt
     2701 ggtggtattg gctttttaaa tggtcgtgct gttacagtta ttggacaaca acgtggaaaa
     2761 gatacaaaag ataatattta tcgaaatttt ggtatggcgc atccagaagg ttatcgaaaa
     2821 gcattacgtt taatgaaaca agctgaaaaa ttcaatcgtc ctatctttac atttatagat
     2881 acaaaaggtg catatcctgg taaagctgcg gaagaacgtg gacaaagtga atctatcgca
     2941 acaaatttga ttgagatggc ttcactaaaa gtaccagtta ttgcgattgt cattggtgaa
     3001 ggtggcagtg gaggtgctct aggtattggt attgccaata aagtattgat gttagagaat
     3061 agtacttact ctgttatatc tcctgaaggt gcagcggcat tattatggaa agacagtaat
     3121 ttggctaaaa ttgcagctga aacaatgaaa attactgccc atgatattaa gcaattaggt
     3181 attatagatg atgtcatttc tgaaccactt ggcggtgcac ataaagatat tgaacagcaa
     3241 gctttagcta ttaaatcagc gtttgttgca cagttagatt cacttgagtc attatcacgt
     3301 gatgaaattg ctaatgatcg ctttgaaaaa ttcagaaata tcggttctta tatagaataa
     3361 tcaacttgag catttttatg ttaaatcgat actgggtttt accataaatt gaagtacatt
     3421 aaaacaataa tttaatattt agatactgaa ttttaactaa gatta
//
LOCUS       DBNENW010000197         3413 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNENW010000197 DBNENW010000000
VERSION     DBNENW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.201"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..785
                     /locus_tag="AC1LW8_002533"
     CDS             <1..785
                     /locus_tag="AC1LW8_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774290.1"
                     /translation="VTERLKQSWKSFLIVLAACLILIIASETLFSFSHLTDVKEVRWL
                     FRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATPVVIMSILSFIMLVILTMI
                     SALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLLTEKINIIFYGVCVLCFLL
                     LPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISSMENIPYHFYFILFLCLIA
                     AVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADTSS"
     gene            complement(1046..2023)
                     /locus_tag="AC1LW8_002534"
     CDS             complement(1046..2023)
                     /locus_tag="AC1LW8_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIP4774291.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            2175..3212
                     /gene="mutY"
                     /locus_tag="AC1LW8_002535"
     CDS             2175..3212
                     /gene="mutY"
                     /locus_tag="AC1LW8_002535"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIP4774292.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRREKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         1176 a          493 c          582 g         1162 t
ORIGIN      
        1 atgtaacaga acgtttaaaa caaagttgga aaagttttct aatcgtatta gctgcatgtt
       61 taatacttat tattgcaagt gaaacgctat tttcctttag tcatttgacc gatgttaaag
      121 aggtgagatg gttatttaga atcattgtat tgattgtttt tgcggtagtg atgttcacaa
      181 tttatatctc ttatcatcat tatatgaatg actttttagt tactaagtta tttaatattt
      241 ccgcagcgac gccagtagtt attatgtcta ttttaagttt tattatgcta gtcattttga
      301 cgatgattag tgcattggtt aaaccagtaa acttcgaaac atcatatata gcgctattct
      361 attttattgt gatggcaact atttttgtag gattaatatc cgttactttt ggtcttattc
      421 gactgttaac tgaaaaaata aatatcatat tttatggggt ttgtgtattg tgtttcttat
      481 tgttaccaat tattttcata ccaaatccaa atcatgtatt tatcaatcac atcctaatgc
      541 taaatccaat gtattacatt gttaatggta tagcacaatc tatcatattt ggtatatcaa
      601 gtatggaaaa cattccatat catttttact ttattttatt cttatgttta atagctgcag
      661 taaatttcgt attagcaagg tatacgacac acgcaattta taataaaaca tctaaagtga
      721 cacaaactga taatcaacag gatgtttcaa atgatagtac agacgaggcg gatacttctt
      781 catgatgttt agctgacctg ttgtgtaaat taatctgtga gaatcagaaa caattagaat
      841 atcatctaga tattgttttc ctatactgat tttgatattt cgagtcatta aaggtagttt
      901 gttagcaaca atataaattt tagattttgt aaaagcattc atagttttac attaaaaaac
      961 cggacagagt aaaaatcgtt ttgattttca ttctgcccgg ttgttcattt tttatagatt
     1021 aactttagtg aataataact tgaaattatt tagcatacag tttacgttgt aacttatctt
     1081 ctgtaaatac ccagccaata taagagtggt ctatgacatt atcgtttgtt acatgtgcaa
     1141 ttgccacaaa tgaataatga tcatttttta aataacgcaa atcaatcaaa cgaatttcag
     1201 tagaaccatc tgctaacgtt gttgtttgcc aacgatagat tgatgaaaag tttaaaaaag
     1261 tacgtatatc tttattacct ttgactttcc ataaaatgga gtctgttgat aatgtttgac
     1321 gttccacttt atcactaaac accacattcc taccatatgc ttttccaaca tagtcatgtg
     1381 catcagtttg tatcgcgaca cgccattcca taaattttat tgttggcgca acaaatactt
     1441 taacagggtg atgctcttgt tgaattgctt ttaaagcttg ttgcttaatt acggctctca
     1501 ttttaaaacg aatcatgtaa taaatgatta gtaaagcgat tataggaaag aagactgcaa
     1561 atggatgcaa gcctataacc cataatacaa taccaataca aagaactgtg aaaataatag
     1621 ggtcaaatgt gttaatcaca cttaattgaa tccatttgtt tgtgataggt ctaagcgctt
     1681 gtgtaccata agaattgaat atatctacaa agacatgtaa aaatactgcg agctgagccc
     1741 acatccatac atgaaatggg ttggttccac taaagaacgt gaatattaaa aatgtaatta
     1801 aaattggcca tagtattgtg aaagggatgg aatgcgtgat acctctatga tgcgaaatat
     1861 atgttgcatt gtcctttaat ttaagaacag tatccccatc aggaattaat gaaccaacga
     1921 taagggttgt agctgttgca ccaaacgtag aagccattgc tggatcttga gttgcaagtg
     1981 ctgtaaggcc caccccaatt gcgatatgtg ttgcagtgtc catatcagtt cactcatttc
     2041 ataatattat atatactatt ataatccaat ttcgcaaata actaaattaa cttacatact
     2101 aaccatgatt ttaatgtaca ataaaaacat tatgagtaaa gacaattaat tttggtgagg
     2161 aaagggtttt ataaatgtat cagcagtcta gttttaaaga aaatttgata cactggtttg
     2221 atgaaaatca aagagaaatg ccatggcgac aaacgaccaa tccgtactat atttggttaa
     2281 gtgaggtcat gttacaacag acacaagtta aaacagttat tgactattat catcgttttg
     2341 ttgaacgatt tccaactgtg gaagttttga gtcaagcttc ggaagatgaa gtgttaaagt
     2401 attgggaagg cttaggatat tatagtagag ctcgaaattt tcatacggcg attaaagaag
     2461 tttatgataa atatgaaggc ttagttccca aagatcctga tcaatttaaa gcgttaaaag
     2521 gtgttggccc atacacacaa gctgcagtaa tgagcattgc atataatgta ccactagcaa
     2581 ctgtagacgg aaatgttttt cgagtatggt ctagattgaa tgatgactat cgagatatta
     2641 aattacaatc gacgaggaaa tcgtacgaac aagaattgtt accgtatgtt actacagaag
     2701 ctggaacgtt taatcaagct atgatggaat taggggcatt aatttgtaca ccaaaaaatc
     2761 cattatgctt attttgtcca gttcaagaaa actgtgaggc atttgataaa ggtacatttg
     2821 aaaagttacc tgttaaatct aaaaatgtaa gtaaaaaagt aattgagcaa tccgtctttt
     2881 taattagaaa taatcaaggt caatatctgt tgcaaaagcg tagagaaaaa ttattgcatg
     2941 gtatgtggca atttcctatg tttgaaagtg agcatgctag acgtaaaatg actgaaaaaa
     3001 tcggtcatga cattcaacca gtagaaacac ctatttttga gttgaagcat caatttactc
     3061 atttaacatg gaagattaaa gtatacgccg tttcaggagc aattaacatt gaaacattac
     3121 cagatgacat gatttggttt gatttaagtg atcgagatca atatacattc cctgtaccta
     3181 tgtcaaagat ttatcaattt ataaatgggt aacattcgat gattggtaat attatcggaa
     3241 aacatagtga cattaatttt aaaaagtata aatgatatct ttaaatctca tagaataaca
     3301 atgtcaaaaa gttaaagtgt tgatatataa aagtgattat tttatatact ttaaatggga
     3361 catgtaaatc tctgttgtag tacttagcaa tattaccgag tttaattatt aag
//
LOCUS       DBNENW010000198         3388 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNENW010000198 DBNENW010000000
VERSION     DBNENW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.202"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            559..1251
                     /locus_tag="AC1LW8_002536"
     CDS             559..1251
                     /locus_tag="AC1LW8_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HIP4774293.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKAIEQSRLSVTSKDTQ
                     ELKKYYSGTGYNFQNVSGYREGNKMNIIDGPQLNVVTLLGTDKERFKDDEDYEGLDVF
                     VVREGSGKHADNISIGGITKTNKNQYKDPVQNVNLLTSKSNGQNTASVTSEYYSINKE
                     EISLKELDFKLRKQLIDKHDLYKTEPKDSKIKVSMKNGGYYTFELNKKLQPHRMGDTI
                     DSRNIKKIEVNL"
     gene            1273..2775
                     /locus_tag="AC1LW8_002537"
     CDS             1273..2775
                     /locus_tag="AC1LW8_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HIP4774294.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGEAEASEGSQTVKNPKV
                     NATEEIKVGSQPVQNNQEVSSEQTKKNFVNLDPIKPGAQKVTGTTLPSHIILMNIDGK
                     SADSVDGGNSDLVFADENGRFEYPLHNRKIVHNQEIEVSSSSPDLGDDEEDEEVEEDS
                     TEKAGTEEENTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHT
                     SVKGKVALSINNKFINFEERAKDGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDN
                     LELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDV
                     KEDLHVDEIYGSLYHTEQGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQ
                     VDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGELLHQGFVSKYIDVYESPEEKKER
                     EFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERKAPKLEK"
     gene            complement(2882..3190)
                     /gene="spn"
                     /locus_tag="AC1LW8_002538"
     CDS             complement(2882..3190)
                     /gene="spn"
                     /locus_tag="AC1LW8_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HIP4774295.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYSNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1331 a          431 c          605 g         1021 t
ORIGIN      
        1 aaattgatat ctatacaatt gccacttctt gaagaacaac agaaaataag agggtttcta
       61 gaagttttat ctggaataac tactaaacaa ttgcacaaga tagaccaatt aaaagagagg
      121 aaaaaggcgt ttttacagaa aatgtttatt tgatttgtcg ctgcaatata gtttttatta
      181 tttgtttatt tcagatgttt caccatcata ttgcgtaact tttacaaata agaaataaag
      241 ttcaatgaaa tcaaaaacga acattaaatt taggcactgt gatagcacag tgtctttttt
      301 gtgtcgaaat tgtgtacaga ataagtagtt aaataaagat taagttgaga taaagtgtta
      361 ttcgtaaata aaagagagta gatcgatagg aattgaatga tattagttaa ctatttatta
      421 aattacttaa taatgattaa tttttagtta aagtaagttt aatgtgaagc acgaccattg
      481 ctcattataa tgaatgagga ttgttcgtat tgcgtaatag aataaatcaa atagactaaa
      541 aattgggagc atagaattat gaaattaaaa aatattgcta aagcaagttt agcactaggg
      601 attttaacaa cagggatgat tacaactact gctcagccag taaaagcaat tgagcaaagc
      661 agattatcag ttacttcaaa agatacacaa gaattaaaaa aatactacag tggaacagga
      721 tataattttc aaaatgtgag tggttataga gaaggtaata aaatgaacat tattgatgga
      781 ccacaactta atgtagttac tttacttggc acagacaaag aaaggtttaa ggacgatgaa
      841 gattatgaag gacttgatgt atttgttgta agagaagggt caggtaaaca cgcagataat
      901 atatcaattg gtggaattac aaaaacaaat aagaatcaat ataaagaccc tgtacaaaac
      961 gttaatttat tgacttctaa gagtaacggt caaaatactg cttctgtgac ttcagaatac
     1021 tatagcatca ataaagaaga aatttcatta aaagaacttg atttcaaact aagaaagcaa
     1081 ttaattgata aacatgatct ttataagaca gagcctaaag acagcaaaat taaagtttct
     1141 atgaaaaatg gcggctacta tacgtttgaa ttaaataaaa aattacagcc tcatcgcatg
     1201 ggtgatacga ttgatagtag aaatataaag aaaattgaag tgaatttata ataatattcg
     1261 agggagtata tcatgagaga aaattttaaa ttacgtaaaa tgaaagtcgg tttagtatca
     1321 gttgcaatta ctatgttata tatcatgaca aatggcgaag cagaagcatc tgaaggtagc
     1381 caaactgtta agaacccaaa ggtgaatgca actgaagaaa tcaaagttgg atcacaacca
     1441 gttcaaaata atcaagaagt aagttcggaa caaacaaaaa agaattttgt taatttagac
     1501 cccattaaac ctggtgctca aaaggtaaca gggactactt tacccagcca tattattcta
     1561 atgaatatag atggtaaaag tgctgattca gtagatggag gaaatagtga tttagtattt
     1621 gctgatgaaa acggaagatt cgagtatcca ctacacaata gaaaaattgt tcataatcaa
     1681 gaaattgagg tttcgtcatc cagtcctgat ttaggtgatg atgaagaaga tgaagaagta
     1741 gaagaagatt caactgaaaa agctggtact gaggaagaaa acacagatgc taaagctaca
     1801 tacacaacac cacgatatga aaaagcgtat gaaataccga aagaacaact aaaagaaaaa
     1861 gatggacatc accaagtttt catcgaacct attactgaag gttcaggtat tattaaaggt
     1921 catacgtctg taaaaggtaa agttgctcta tctattaata ataaatttat taattttgaa
     1981 gaaagagcta aagatggaat tagtaaagaa gatactaaag ctagttccga tggtgtttgg
     2041 atgcctatta atgaaaaagg atattttgat tttgatttca aaaaaaatcc ttttgataac
     2101 ttagagttaa agaaaaatga tgaaatctca ttaacatttg cacctgatga tgaagatgaa
     2161 gcattgaagt cattaatttt caaaactaaa gtaacgagtt tagaagatat tgataaagca
     2221 gaaactaaat atgaccatac taaagtggaa aaagtaaaag tattgaaaga tgttaaagaa
     2281 gatttacatg tagatgaaat ttatggaagt ttgtaccata cagaacaagg taaaggtatt
     2341 ctcgataaac agggaactaa agaaattaca ggtaagacta aattcgcgaa tgcagtagtg
     2401 aaagtatatt ctgacttagg tgatgcgcaa ctgtttcctg atattcaagt agatgaaaat
     2461 ggtaaattta gctttgatgc tgaaaaagct ggtttcagat tacaaaatgg agaaacattg
     2521 aattttgcag tagttaaacc tattactggt gagctattac atcaaggatt cgtttctaag
     2581 tatatcgatg tttatgaatc tccggaagaa aagaaagaac gtgaatttga agagaaactt
     2641 gaaaacacgc ctgcatatca taaattacat ggtgataaaa ttgtcggcta tgatgttcaa
     2701 ggtaatccat caacttggtt ctatccatta ggtgaaaaga aagttgaacg taaggcacca
     2761 aaattagaaa aataattaat aaaataaaac agcttaatga tgtaatgaaa ctagtgagtt
     2821 aatcactgac ttcaacgtca ttgagctgtt ttttgtgctt tgttacaaag cattattgag
     2881 tttattttac gtgttcatat tttgaaacat caaagccgtc ttgtttagct ttgttgataa
     2941 tgtctttgat tgaatgtagt cctttatcgg cgaagtatga tcttaagttg tcttttgtag
     3001 cttggtcagc attcttatct aataacacat cgatatagct taattcatgt tctaagaagt
     3061 ttgcatcatc atgtagtaca agtccatttt gagaataaac tttcgcatct gcttgattac
     3121 tatatccaac aacgccagtt gctaaaacac ctaccattgc cgtagctact aaaacctttt
     3181 taaatttcat atctatcact cctctaaaaa atgtaactct atcataacac tgaatattga
     3241 gaaaattacg tttattaagc cgatttaata attttaataa atagttaaag tgacaaatat
     3301 tgtttaaatg caattaatct ttaatgcgat ggttatggat tttcctacta aaaccttgat
     3361 ttcaaagagg gtttaaatca aatgaaac
//
LOCUS       DBNENW010000199         3360 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNENW010000199 DBNENW010000000
VERSION     DBNENW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.203"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..688)
                     /locus_tag="AC1LW8_002539"
     CDS             complement(<1..688)
                     /locus_tag="AC1LW8_002539"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4774296.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPAKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRCNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQ"
     gene            complement(681..1454)
                     /locus_tag="AC1LW8_002540"
     CDS             complement(681..1454)
                     /locus_tag="AC1LW8_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP4774297.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(1441..2271)
                     /gene="nikC"
                     /locus_tag="AC1LW8_002541"
     CDS             complement(1441..2271)
                     /gene="nikC"
                     /locus_tag="AC1LW8_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HIP4774298.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(2264..3250)
                     /gene="nikB"
                     /locus_tag="AC1LW8_002542"
     CDS             complement(2264..3250)
                     /gene="nikB"
                     /locus_tag="AC1LW8_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HIP4774299.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISVSSDRHA"
BASE COUNT         1168 a          600 c          480 g         1112 t
ORIGIN      
        1 cttgcactaa ttcttttgta taagggtgtc tatcaacatt aaataattcc tctattgcaa
       61 aatcatcgac tatcatgcca tccttaagaa cgataattct attaactaag cgttgtaaca
      121 cggataaatc atgagaaata acgataaaat gatttaagtt cgtaatcgtt tgcgctttta
      181 atatatttat tacattttgt tcagctataa catctaaatt tgaagttatc tcatcacata
      241 ttaaaacgcg aggctgtgct aataacgaac gcatgacatt acatctttgt aattgtccgc
      301 cactcacttc gcttggtaat ttagtcaata attgcgcgtt taactcaaaa gtagataaat
      361 gttgtaataa taattgatcc tgagcagtat tatcagttag gcctctgtaa taatataacg
      421 cttcttttaa tgaggtctca atcgtccaat cagggttaaa gctagttaaa gggtgttgga
      481 aaatcggtaa cacagcattg tcacttaagt aaatttctcc tttagcaggt ttaaacaagc
      541 caagaaccaa tgaagcgagc gtacttttac cacagccact ttcgcctaaa ataccaacat
      601 tttctccatc aggtatagta atattgatat cttgtagcac catctgcttt ttattataac
      661 caaaagtcac atgttttaac tcaatcatag taatccctct ttaattgtgt tctatattta
      721 attagacgtt cagtatacgg atgcaaatgc tcatacttga aatgattaat attacctcgt
      781 tcaatgattt gaccttcttt taaaacataa atgtactgac aatatttcaa tacatgactt
      841 aagttatgtg tgataataaa taatgtttga ccatgttcta atacaatatg ctgtaataaa
      901 tccatcactt gattaccgtt caaagcatcc aatgatgcaa ctggttcgtc tgcaatgatt
      961 aatttaggct ccaacatgag aacgcttgct atgtatacgc gttcaagttg gcccccagaa
     1021 agttggaaac tatatttatt taatatatct ttgctttgta aattaaccca cgacaaagcc
     1081 ttatcaactt tggacaaagc ctcttcttta ctacctttat aatgcttacg ataaatcgca
     1141 gttaactgtt tacctaattt agtatggtcg ttaaaacttt ctgcataatt ttgagaaata
     1201 tagccaattg tatgaccata atattgactc aatctactaa cattttcccc atcaaattgg
     1261 tacgaatcat acgtgcagct taaatcaaat ggtaaatatt caagtaaagc tttagcaatc
     1321 aaactttttc cagcgccgct ctctccaatc aaggcattaa tctgttgact aaaaattttc
     1381 aaatcaatcc ctttaataag agatttctca ctagtattct ttattgttaa attttgtata
     1441 tcaatgagac tcatcatatt caccccgttg tttcagcaat ctatctctta gtgcatcacc
     1501 ggttaaatta aaaattaaaa tagttatagc aatgactgaa gcaggtgcaa tcaacataat
     1561 tggatgagac gaaataaaat cacgaccttg ttgcaacata gcgccccact ctggtgttgg
     1621 cggttgtgca cctaacccaa taaatgatag tgaacttata tatagaatga ttttaccgaa
     1681 atcaacgacc atcaaaacga taatagccgg tataatttta ggtgttaaat gacgtattaa
     1741 tattgttctt gttggtacat gaaataattg tgccattttt atataaggct tattcatttc
     1801 gctattaact atacttctag tcaaccttgt gtaattcatc cattttatta atgtaattga
     1861 gataactaaa ttccataaag atggttgaaa aaaacttgct aaagcaatca tgatgataaa
     1921 ttctggaata ctcagaccaa catcaataaa ccttaacact aatcgttcaa tccacccttt
     1981 tttatatccg gcaaatagac ctagtgtaac acctatgaca acgatagcta ttaatgttaa
     2041 aacagtaaca aacaatgttg aacgtgcacc gataataatt cgggtaaata aatctctccc
     2101 ataatcatca gttcctaata aatgcaacca actaataggt tcaaaagttt gtgataaatt
     2161 gactttggtt gcattttcac tactgacaaa gaattgcagt acaattacca caaaaataaa
     2221 tgcaacgaat acaaaaaata tcaggttatt ctttgaaaat attttatgca tgacggtcac
     2281 tacttactga tatcaatggt gtattggttt tgatttttgg atttcctaat tgtaaacgct
     2341 gcttcggatc aagtaataac gttaataaat cagcaatcgt attgataata acaacgaaga
     2401 agccaataaa taacacgcat ccttgaataa caggataatc tcgagattta atactatcca
     2461 ttaatagata accaatacca ggtatatcaa ataaattttc aatcactaca gtaccaccta
     2521 ttagactgcc aagtgaaatc cctagtaatg ggataatcgg caaaattgtt ggttttagta
     2581 aatcatgaat taaaatataa cgttcattca taccgcgtaa tcttgatgct tgtacgatat
     2641 tactttgcaa taacatcaat aaattagaac gcactaaacg aatgatgtat gcacacatac
     2701 ctaaagatag cgtgattaca ggtaatataa actgacttag tataacgcta tctatattca
     2761 ttaaatttgt gacaataaat aataaaataa taccgataaa gaacgctggt aaactaatcg
     2821 atagtgttga gatcactcta atcactttat ccgtccactt atgaaatcgt ttggctgcta
     2881 taatgccgag cggtatagat atgcataacg acactactaa tgttgaaaat gatatgagta
     2941 atgttatggg tgcatagttg aataatatct gtgttaccgg ttcttttgat tcaaaacttt
     3001 ttcctaaatt aaaatgtaat aaatgattca tccaatgcca ccactgtacc aataaagaat
     3061 catttaatcc caatttatct ttggttgcat ttatttgttc cgtcgacact tgtgctacat
     3121 caagatgtaa tattttatca acaggattgc ctggtgataa tttcattaaa atgaatgtaa
     3181 gtgtagaaat aacaaataaa acaactatca tttgcatcag tctatacaac atagacttta
     3241 ttatgaacat aatagtcccc ctccttgtgt aagttactaa cactttcttt ttacatgaga
     3301 atggcgcatg tatatgcaac ttacatatta agaaccaacg ttcattatag tattatccat
//
LOCUS       DBNENW010000200         3358 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.204, whole genome shotgun sequence.
ACCESSION   DBNENW010000200 DBNENW010000000
VERSION     DBNENW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.204"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..101)
                     /locus_tag="AC1LW8_002543"
     CDS             complement(<1..101)
                     /locus_tag="AC1LW8_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIP4774300.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNG"
     gene            complement(105..1229)
                     /gene="purK"
                     /locus_tag="AC1LW8_002544"
     CDS             complement(105..1229)
                     /gene="purK"
                     /locus_tag="AC1LW8_002544"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIP4774301.1"
                     /translation="MSFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(1216..1698)
                     /gene="purE"
                     /locus_tag="AC1LW8_002545"
     CDS             complement(1216..1698)
                     /gene="purE"
                     /locus_tag="AC1LW8_002545"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIP4774302.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1899..2759
                     /gene="folD"
                     /locus_tag="AC1LW8_002546"
     CDS             1899..2759
                     /gene="folD"
                     /locus_tag="AC1LW8_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIP4774303.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
BASE COUNT         1074 a          569 c          573 g         1142 t
ORIGIN      
        1 ccgtttccag cagtaacttc atctttatat tcaactctta attcattttc ttgatttgtt
       61 gagaaaatgc gcttcgcttt tccttcatat aataatgtca tgctttaatt actcccctca
      121 aatttagcgt acatatcttg ttcagtttgg tttacatcat tcgttagtac agtcatatgc
      181 cccatttttc tgctatcttt acgctcagac ttaccataaa tatgtaagtg ccactctgga
      241 tgttcattaa attcattttc caataaatct aaatctttac ctagtaagtt catcatgact
      301 gctggcttta ataattcaat tgaatttggt aatgattgtc cggtaactgc taaaatatga
      361 gtatcaaatt gtgaataatc acatgcttca attgaataat gtccggaatt gtgaggcctt
      421 ggtgctatct cgttcacata caattggttg ttactatcta taaaaaattc aactgtaaat
      481 gttccaatga aatgaatcga ttggataatt ttattaactt gctctttcgc ctcagctgtt
      541 ttatctattc tcgctggaac aattgttttg aaaagtattt gatttctatg ctcattttct
      601 tgtaatggga aaaaagtgat ttgattgttg tttcctcttg taacagtaag agatacttct
      661 ttcttgatat tcaaatattt ttcagctacg cattcactag tttcaattaa tttaaaacct
      721 tcttgtaagt ctttttcgtt gttaattaaa acttgacctt tgccatcgta gccaccaaat
      781 ctagttttta caataaaagg atatcctaat gtttcaattg ctttgtcaat atctgtagat
      841 tcttttactg aaatgaacgg gacaactttg gtaccagcac tttttaatgt ttctttttca
      901 gttaagcggt cttgtaataa ctgtatagct tggtaacctt gcggaatatt gtacttttca
      961 cataatagtt ttaattgttg ggctgaaatg ttttcaaatt cataagtaat cacatcacat
     1021 ttttgtccta attgattgag tgccttttca tcgtcatact tggcttgtat aaattcgtgt
     1081 gcaacgtatc tacatggaca atcttcagaa ggatccaata caaccacttt ataacccatt
     1141 ttttgagctg attgtgccat catctttcca agctgaccac caccaataat gccaatagtc
     1201 gcaccaaact ttaatttatt gaagctcatt ttgcatgtcc tccacttttt gaattaacga
     1261 agattcatac tgatttagtt tttcaactaa agaaggattt tgaatactta acattcttgc
     1321 tgcaagtata cctgcgtttt tagcacctgc tgcaccaatt gcagtcgttg caaccggaat
     1381 acctcctggc atttgaacaa ttgataataa agaatctata ccctttaaac tttttgtttc
     1441 aatcggcaca ccaataactg gtagcgtcgt taatgatgca accatacctg gtaaatgtgc
     1501 cgcaccgcca gcgcctgcaa tgataatgtt tatacctctt tctctcgctt cagaagcaaa
     1561 ttgaaccatc atttttggcg tacgatgtgc ggatactact tgtttttcgt acggaatttc
     1621 aaaataatcc aacatgttac aactctcttg cataattttc caatcggaag aactgcccat
     1681 aatgactgct actttcactt tgtacaccct ttcaaaagtt tgaattgtga attactttag
     1741 ttgtatatta tagatatagc ataacaagca atttctgctt tttcaatcaa aaatcgaact
     1801 ttattttgat tttttgtttg aatttacgtc ttttgctatg taaattagtt ttatgaacta
     1861 acaaagttag catattgaca ataggaggag aagtttttat ggttgctaaa attttagatg
     1921 gtaaacaaat tgccaaagac tacagacagg ggttacaaga tcaagttgaa gcgctaaaag
     1981 aaaagggttt tacacctaaa ttatccgtta tattagttgg taatgatggc gctagtcaaa
     2041 gttatgttag atcaaaaaag aaagcagctg aaaaaattgg tatgatttca gaaatcgtac
     2101 atttggaaga aacagctact gaagaagaag tattaaacga actaaataga ctaaataatg
     2161 atgattctgt aagtggtatt ttggtacaag taccattacc aaaacaagtt agcgaacaga
     2221 aaatattaga agcaatcaat cctgaaaaag atgtggacgg atttcatcca ataaatatag
     2281 ggaaattata tatcgatgaa caaacttttg taccttgcac accgctcggc atcatggaaa
     2341 tattaaaaca tgcagatatt gatttagaag gtaaaaatgc agttgtaatt ggacgaagtc
     2401 atattgtcgg acaaccagtt tctaagttac tacttcaaaa aaatgcatca gtaacaatct
     2461 tacattctcg ttcaaaagat atggcatcat atttaaaaga tgctgatgtc attgtcagtg
     2521 cagttggtaa gcctggttta gtaacaaaag atgtggtcaa agaaggagca gtaattatcg
     2581 atgttggcaa tacgccagat gaaaatggca aattaaaagg tgacgttgat tatgatgcgg
     2641 ttaaagaaat tgctggagct attacaccag ttcctggtgg cgttggtcca ttaacaatta
     2701 ctatggtatt aaataatact ttgcttgcag aaaaaatgcg tcgaggtatt gattcgtaaa
     2761 tttacattag ttatgtattg agaattaata actgagtttt aatgaagtct tttaacccac
     2821 atttaatatt atttcgcaat tgaaatgaat aacaagaaaa atctgggaca ttaatcgatc
     2881 aaatgctccc ttcaaagtag acattgaata aatgaaggct ttgaagggag catttcactt
     2941 tgttcttggc tcaacaattt tatatagaca gtagttaatt gaatgaaaat aagcttgtaa
     3001 caagttttca attctagtca ggggccccaa cacagagaat ttcgaaaaga aattctacag
     3061 gcaatgtgag ttggggtggg ggccccaaca aagaagctga cgaaaagtca gcttacaata
     3121 atgtgcaagt tggggagggg tcccaacaca gagaatttcg aaaagaaatt ctacaggcaa
     3181 tgcgagttgg ggtgggggcc ccatcacaga agctgtgact atgataaagt actactacat
     3241 agttaatcat tagtggttct ttatcatttt cgcctccctt ttcttattgt tttgaaacac
     3301 aaaaatttaa gttcaaactg tcgaataaag ttatatttga tttcaaatta tccctaaa
//
LOCUS       DBNENW010000201         3312 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.205, whole genome shotgun sequence.
ACCESSION   DBNENW010000201 DBNENW010000000
VERSION     DBNENW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.205"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(171..794)
                     /locus_tag="AC1LW8_002547"
     CDS             complement(171..794)
                     /locus_tag="AC1LW8_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP4774304.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(816..1928)
                     /locus_tag="AC1LW8_002548"
     CDS             complement(816..1928)
                     /locus_tag="AC1LW8_002548"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIP4774305.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLIHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGRVTFHSQPTKGTQIQFTIPIK"
     gene            2091..2912
                     /locus_tag="AC1LW8_002549"
     CDS             2091..2912
                     /locus_tag="AC1LW8_002549"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP4774306.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
BASE COUNT         1074 a          556 c          489 g         1193 t
ORIGIN      
        1 gtaaatgaag taaaatttta tgcataatca ctagatttga taaaaattac acttttataa
       61 tgatatcaaa tttacaaaga aaactgtata ataaacccta tcgctataaa aaattcatca
      121 aaatcagatt aaaaattgtc tatttttcga caaatattta aaagctcatt ctaaatcaac
      181 ttattttcca ttgcataaat tgctgcttgt gtacgatcgc taacttgtaa tttactaaat
      241 atatgactga catgtgtttt aattgttttt tcagatacaa ataaagtttc tgcaatctct
      301 ttatttgttt tacctttaac catttcacgt aacacttcaa tttctctctt tgacaactta
      361 ttcgtgtagt gtggtttttg gctaactgtt tcgaatacat cttgtgcctt aggatgtatc
      421 attttttcac cgttcataac tcgtctaata gtttcaatta attgctgagg ctcaacgtct
      481 ttcatttcat aaccatcagc acctttattg attgctgaaa ttacatgttc atcatcaaca
      541 taacttgtta ataccaaaac tttaatatcc ggataatgtg ccttaatata ttccgtaatt
      601 tcaataccat tcatgccagg catcactaaa tctaatagca caatatcagg gtgctcatgc
      661 tcttttaaat attctaaaaa tgtttctcca tctgcaaagt cttgtaaaac ttctatgttt
      721 tcaatcgtgg ataataaaaa tcgcaatcct tgtcgcacaa tataatggtc atctactaat
      781 attactttgt tcatgggtta tctccttaaa tcaagctatt ttataggaat tgtgaattgt
      841 atttgtgtac cctttgttgg ctgagaatga aaggtcactc tacctcttaa taatttaact
      901 ctttgtttta tgttattaat accgtgtgat gaagctatct gaacattatc gatctcaaat
      961 ccttgaccat aatcaatcac gtcaatatat agtatatcgt tcatttgttt taatgtaaga
     1021 tccattttat tcgtatcagc atgtttctta acattattaa tacactcttg taatgctcta
     1081 tatatgtttt cttcgatttc attagataaa tcgattaaac cttctacatt tacatttaat
     1141 tgtatatgga ttaatttact atatgctgtc aaagcatgaa ttaaaccttg ctcaagtcca
     1201 actggcttaa gttgccaaat caatgcacgc atttcattaa cggcattttg actcgtttcc
     1261 tcaatcgtct tgaatgcttg tttagcgatg gattcgtttg acatgccata cgcagcatgt
     1321 gctgttagtt ttacagaaaa taacatttga tttactgaat catgtaaatc tctagctaaa
     1381 cgattacgtt cattaatttt tgctgcttct ttttcacggt ctgttaaata aatacgtttg
     1441 atggctgacc ctaattgaaa tgcgacagac tctagcaact ctaaatcttc atcactatat
     1501 atttcagtat ttggtgacgc tacatttaaa atgccgaatt gttcttgacc cgatttaagt
     1561 ggtaccgttg catgatgtgt aatattgtca ttttggctag gaaatgcttt agaggctaag
     1621 ttaatacgag aacaattgac gatattcgac gctttcatta gcctacgttg attaaatgct
     1681 ttcacacacc aacaagaccc atctttaata tagtgacagt ggtctgctgt caaagattgt
     1741 ggtagagcca catgtgatac aagttcatgt tcacctacgc tattgatgaa aaatatccag
     1801 cctgtcgtga aattactgcc ctcaattaaa tattttaacg caccttgggt catgctatac
     1861 atttcagttt cttcgtttaa aaattcggca atctctttta ataaagctag tcgcgtcctt
     1921 tgttccatca aatcgctcca attcattttt acgtgtatta actattatac attgagttat
     1981 tatattttta aatcttagac gtgaacatga taaaatggcc ttgattactc aatagttata
     2041 tttcggagaa ctgatttgtg atatgatatt aaagactata ggaggatttt atgaaattta
     2101 aaataccaga aaactttaat gacttaagtt tacgagatat tttccaacaa cttaaggtac
     2161 ctaaaaaaga tttacatcat ttaaatatgt ctaaagatat tactattaat gataaacctg
     2221 cgcgattaat ggataaagtg catactggcg acgatgtatt tgttccaacc atcgatgaaa
     2281 aaagtaatta tgttccaagt tatcgttatg cacaaattaa atacgaagac gatgatatgg
     2341 caatcgtaat gaaacctaaa ggtgttaaga ctcaccctaa tgatttaaaa gaaagcaata
     2401 ctttaatgaa tcatgtgatt tacactattg atagtgacta tgtcgaacca attcatcgac
     2461 tggaccagga aacagtagga ttattaattg ttgctaaaaa tcctttaatg aaaaaaattc
     2521 ttgatcgcat gttagaagac aatgatatta cgcggatata caaagcaaat gttaaggcac
     2581 ttttaccttt gaaaccacaa acgattgata tgccaattgg taaagataaa ttccattcga
     2641 ataaacgacg tgtgtctcct actggacagc gtgcaattac acacatttta acttcaaaaa
     2701 tgataaaaga agctgtgtgc caacttgaaa tcaagttgga tactggacgt actcatcaaa
     2761 ttcgtgtgca tttagctgaa attggtcacc ctgttattgg tgatccttta tatggtgatt
     2821 caacgttaag acaattagaa cttgaaagtt acaaaataga gtttgtgcat cccttgacta
     2881 aggaagtcat ttccgtttct ttggatgact aatttgatta gttttgcatg atatttaaac
     2941 atgcaatacc gcattgtaac taaatcaagt atcaacttaa atcggataga tggaaaatta
     3001 ttaatttttt cagatgttcg gttttttgtt ttttacgatg cttaggattt tatattttga
     3061 tattttagta attattcatt ttataacatc cttggataat aacttgtagt ctttttcaac
     3121 tgcgttacgt gtatctatgg acaatacatg acatcataag atttttatca caggttgttt
     3181 ggccaataca tgtacaacaa ttcatcatat aaaaaatagg ttctataata aaacggactc
     3241 catgaaaagt tttttctttc acggctctat atcaaatcag acttataagt tcaatttgac
     3301 atagacaaaa aa
//
LOCUS       DBNENW010000202         3172 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNENW010000202 DBNENW010000000
VERSION     DBNENW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.206"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1301
                     /locus_tag="AC1LW8_002550"
     CDS             <1..1301
                     /locus_tag="AC1LW8_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIP4774307.1"
                     /translation="VHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFIASS
                     AFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFLI
                     MVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSA
                     FNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKL
                     VLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASIN
                     KSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHI
                     KLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGIT
                     KIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(2146..2589)
                     /locus_tag="AC1LW8_002551"
     CDS             complement(2146..2589)
                     /locus_tag="AC1LW8_002551"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774308.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(2695..3131)
                     /locus_tag="AC1LW8_002552"
                     /pseudo
     CDS             complement(2695..3131)
                     /locus_tag="AC1LW8_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1119 a          521 c          376 g         1156 t
ORIGIN      
        1 aagtccacaa acctttatat ttttatttaa tgctattctt ctctacaact atcataggtg
       61 cacttttatt gtatttacct ttcactggta aaaagccaat atctttttta gatgccctat
      121 ttatagcttc aagtgcattt acagttactg gcctatctcc agttgatata gggtcacagt
      181 ttaatatact tggtgaaata gtaatactat tattaattca aataggtggt ctgggtatcg
      241 taaccgtaac cctattgaca ctagtatttt taaatagaaa gatatcaatg aaaaatagat
      301 tcttgattat ggttacatgg aatattgacg aacctggtgg tgttattaag ctaattaaac
      361 acttggctat ttatagttta gtcactgaat taattggtat gatttgtttg tgtttatctt
      421 ttataccaaa atttggtata ggcaaaggat tatttttaag tttattcaca tcagtatcag
      481 cttttaataa tgccggattt gcccttttta agaataactt aatagattat tctagtgatc
      541 caattgtcat tattacaatc tcaatactca taatatttgg aggtattgga cattttgtcg
      601 taatagactt tattaattgt aaaaaattga gtaaattatc tttacattct aaattagtct
      661 taactacaac tagtatccta ataattatag gagctattac attcttttta ttagaacagt
      721 ttaatactat gcaacatatg ggactagttg aaaaaatcgg aaattctttt ttccaatcag
      781 taacaacacg aacagcgggt tttaacagta tagatatagc aagcattaac aaatctaccg
      841 ccttaatgtt aatgctactt atgtttattg gtggtgcccc tctcagtgca gctggaggaa
      901 ttaaaataac tacttttgca gtggcgttta tatttgttct aaattatata cgtaaagaaa
      961 ataatgtttc agtattcaat aaagaaatat ctgacaaaca tataaaacta tctattgtta
     1021 ccattaatat ctcatttcta tttatcagta tcattacttt tatattatcg ataattaatc
     1081 cgaacatatc attaatcaag ttattattcg aagtggtttc tgcatttgga acagtcgggt
     1141 taagtatgaa ccttaccaca gaatatcatg gtattactaa aataattatt atattcgtta
     1201 tgctttgcgg taaagtagga ctattaactt tattaagaac atttatacca ccaaaaagtc
     1261 ctaaaaatta ccgctacact aaaggacaaa tttatctata aaatagctta tccatcttta
     1321 acaagaaatg aactagtgtt ttaatctatt acattaaaaa aactacctaa acaggtagtt
     1381 ttttatttat tttaggccca ttaaatgtct tatgtcttta attaagtaaa atgcacaagt
     1441 acacctttaa aaagatttac tcaaaactaa actataccta gttaaccgct tgtgattaag
     1501 aaattttaaa tgtggtttct tttaattatg tcaaccaccc ataaggattt taatcaagca
     1561 ctcattcatt cctgcactcg caatatgcat tacttataga agtggatgca ctctcatatt
     1621 atggcaatac cataactccc gcaccataat gatttaaata ccattatgaa tctcaattac
     1681 aaattagcac ataaaaaaga cgcctcccat aagtctagtc accaaatgga aaacgtctcg
     1741 taattaaaat atatttgcgt gcattctaat ttgtacttag aatacatcat actcgttcat
     1801 aatgtaattt tctaattagt caaaactata tacagtttta catcattccc ggcattatgc
     1861 actaataaaa acttacttta atacacgctt taaagattat taaatcagcg ttttaatttt
     1921 tagacaacgc tattattttt agtttttcaa ttctattatg tcataattat gtcactcaaa
     1981 aactgttttc caacattgat ttattgttgg aaaatctagt ccatttctcc aacaatcgaa
     2041 gaattatgcc cttttttctc cctttaaata agtcataata cgaggcatac atgcaacatt
     2101 tacaataaaa tatgttccta catcgtatta tacgaatgct cacacttata gttgtttagt
     2161 aaacccagat gtattgctaa catacccatt atcactatat agtttatgtg cagatagtct
     2221 ttcatttcta ttaccactat ttagtgttat tgctttacaa ttcaaccgtt tagaaaattc
     2281 ttcagaatca gctaataatc tctttccata accttttttc ctaaattcag aatgtataac
     2341 aaacgcaagg attctcatat actctgcatt tttttcgtaa aacatcattt tacacatacc
     2401 acttaaacca attattttat tttcttttat caaaagcagt aggaagtaat catcatgatt
     2461 cgttattttc tttagtctct tttttaaatc attctcattt gttggataac ctagatcatc
     2521 atataattta catagttgat ttaatttttc aaaatcactg ttattaaaca atcttgtaat
     2581 tatgctcata taacccaccc taaaaatatt atgtacctat tgttacatac aatagatacg
     2641 caaaatcatt tctcactgca aatcaggacg tttctcagcg taaacaaatc attgctattc
     2701 ttttgaaaaa tatctgcata tcatcacagt aattttataa cagattaata aaattaaaaa
     2761 aacataacaa caagtattat aataaatatt ataatagcta tcgtattaaa tagtttagaa
     2821 tatgcaaact gaaaaataaa aatcacaatt aaaatactaa gtaatgtaaa tggtaatagc
     2881 atcatcagat ctttaacatt atttctttgt tcttgctccc aatcgctaat agccctaaaa
     2941 ctactttttt tatctatagg cgatgtaaaa ataccatata ttgaaggtgc tatacctcct
     3001 aaaatagcag ttcccaaagt tgtcattact gaaattactg cgaaagtatc atccgaaagc
     3061 aataaattca aactaatgca ttgtttatta cccatcgaat ttattgacca aatagctaga
     3121 gaaataaaca acccaaaatt taaaataaat gatatagtaa tagcaattgt tg
//
LOCUS       DBNENW010000203         3145 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNENW010000203 DBNENW010000000
VERSION     DBNENW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.207"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(124..1059)
                     /gene="trxB"
                     /locus_tag="AC1LW8_002553"
     CDS             complement(124..1059)
                     /gene="trxB"
                     /locus_tag="AC1LW8_002553"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIP4774309.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1126..2565)
                     /locus_tag="AC1LW8_002554"
     CDS             complement(1126..2565)
                     /locus_tag="AC1LW8_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIP4774310.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEGSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            complement(2573..3058)
                     /locus_tag="AC1LW8_002555"
     CDS             complement(2573..3058)
                     /locus_tag="AC1LW8_002555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIP4774311.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            complement(3066..>3145)
                     /locus_tag="AC1LW8_002556"
     CDS             complement(3066..>3145)
                     /locus_tag="AC1LW8_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774312.1"
                     /translation="YRRIKYNPPLYSKVGALPWPTKKVK"
BASE COUNT         1007 a          599 c          407 g         1132 t
ORIGIN      
        1 agttctagtt ttctatataa tttgttttaa tttaaaagta agacattatt ttaacaatgt
       61 gatgacacta ttaaaataaa tatccaaata acttacaact caacatctta attcgacctc
      121 gaattaagct tgatcgttta aatgttcaat atattccgct gcactttgcg ctgcaatact
      181 accatcgcca gtagcagtga caatttggcg taaacctttg tcacgaacat ctcctgctgc
      241 aaaaatacct ggtactgatg ttgtcatatc atcttttgtc acaatataac caacatcatt
      301 tgtaatacct aagtctttaa atggcgctgt taatggtttc ataccaatat agatgaatac
      361 accatcagcc tcgtgtgttt cttctgaacc atcttttgta gacgttaatg tcacagaacc
      421 cactttgccg tctttttcat taattgattt caaagtatga ctccaaataa agtcgatttt
      481 atcatttttg aatgctctat cttgtaaaat acgttgtgca cgtaactcat cacgacggtg
      541 aacgattgtt actttgtcag caaatttagt taagaatgtt ccctcttcta ctgctgagtc
      601 accaccaccg ataacgaata ggcgtttatt tttaaagaat gcaccatcac atactgcaca
      661 ataacttaca ccgcgtccac caagttcttg ttcacccgga acaccaattt tcttgtattc
      721 tgcacctgta gcaataatga ccgctttcgc tgttaattct ttattaccaa agttaatcac
      781 tttatattcg cctttatctt ctacagattt aatatctcca tattgataaa ctgcaccaaa
      841 ctttttagcg tgttcaaaca tttttgtaga taaatctgga cctgtaatca tttcgaaacc
      901 agggaagttc tctacttctt ctgtattagc catttgaccg cctggaatac ctctttcaat
      961 cataactgtt tttaaattag cacgtgatgc gtatactgca gcagtcatac cagctggacc
     1021 tgcaccgata attgctatat caaaatctat ttcagtcatt ttattaacgc ctcctcatta
     1081 ttaatcatta tgcgcattat ataataaatc taacttttca taaatctata tgctcaagag
     1141 aaattcaatc attttgttca gtttatattg tgttatgcct aaccatgttg taatttgctt
     1201 ctttgtaacg tttcgaggtt gatttttaaa atacaaataa ataaacgcac cgatatatgg
     1261 ctcaacatca gttaaatcta ctttttcagc aattatgagt tcaccttgat taatccatgc
     1321 aaccattaaa tcattttcac ttacaaataa ttcattttgg taaagcgtta acaagccgcg
     1381 atgaatgaag tctaatttat tgagcgttaa accttgaact aaatacgtta aatacaattt
     1441 ctcataatta tttagatttt ccagaacctg ccaaatactt tccgtcatca caatttcttt
     1501 accatttaat tgatccaata aaaaaatacc atataaacga taatgactgt catcactttg
     1561 aagtaatggc aaaatatgtt ggtcaaaaac ttctttgcta ttttcaatta cccaaggcgc
     1621 atgtccaatt tccactttag aaatttgctt caatttatcc cagtagtaat gactttcgtc
     1681 ttcttcacct aaataataat aattatatgc taaagcattg tacatttgaa ttgataaaaa
     1741 tttccctttt ttatataaag ggtacaacaa ttgttgtgat gcacgatact gctttaaata
     1801 acttaataca atacctaatt taaaactttc atcgtcattc ataggtacaa ctttgtttaa
     1861 tatttttaaa tatttttgat attgttcatt ttccttagtg ttataaagta gcaaagtata
     1921 atggcataat gcatatatat ctgtactatc ctcttgtaat agtgcttcgt acaatgcttt
     1981 agccgtatca tattcaccga gatataaata acacattgcc aataaattac gtacgacacg
     2041 atgatcttga acttcttgtg gttgatgtaa gacataattc tttgcatctt ttaatcgacc
     2101 ttgagaaaaa agatattgga atagtatttg aactacaaac aattgagctt caagttcaat
     2161 ttgttcttcg ctttgatatt tcacatcaaa catttgatct aattcatctc tataatcttg
     2221 gtcgtcgcta acaataacat aattaatacc aaacaagaat gccttgtttg gttcgttaac
     2281 ttcaatgttg agctgactca attcataaaa gctaccttct agatgcctat tataaataat
     2341 attgtcataa aataaatgtt ctgctttttt agcaatacca agttgcacta gacattgtgc
     2401 ataatcaatt tgaatttcca gatcatcagg tgacaattct aacacttttt caaagtattc
     2461 agcagctcgt ttatattctc tttgtttaaa cttctgttta gccattttac gataaaatgc
     2521 atcatcaaaa gtcattggaa ttacattatt attcttttgc gccataaaat tgtcacctcc
     2581 taatcatttt tatatacata gggttgccat atgcaaatcc attatccggt atatcttttg
     2641 aaacaaccgt accagctgca acttttacat tgtcaccaat cgttataccg ggtaaaatgg
     2701 tagcatttgc accaatcaaa gtgttagatc ctatcgtcac tggtccataa cgaaattcat
     2761 caactaatgc ttcatgcgtc aaaattgtta cattgtaacc aataacactg ttactaccaa
     2821 tcgtaatcag ttctgggtaa aaaatatcta acattacttt ataagctatc gacgattgat
     2881 taccgatatt aatatttaaa agttgtttat atatatgtct tttcagtacc atacttggaa
     2941 taaatttcga aaattcaatg ataattacat tcttaaaaac tttcgaaaat ttaacaagac
     3001 ggtatacacg ccataaaggg tttgtatgat gatgtgtttt tgataaaaat tttctcaaaa
     3061 tatcactact tcactttttt tgttggccat ggaagcgccc caactttgct atacaacggt
     3121 ggattatact taatccttct atata
//
LOCUS       DBNENW010000204         3073 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNENW010000204 DBNENW010000000
VERSION     DBNENW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.208"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(26..1344)
                     /gene="cstB"
                     /locus_tag="AC1LW8_002557"
                     /pseudo
     CDS             complement(26..1344)
                     /gene="cstB"
                     /locus_tag="AC1LW8_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            1400..1537
                     /locus_tag="AC1LW8_002558"
     CDS             1400..1537
                     /locus_tag="AC1LW8_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774313.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            1534..1905
                     /locus_tag="AC1LW8_002559"
     CDS             1534..1905
                     /locus_tag="AC1LW8_002559"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIP4774314.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            2034..2654
                     /locus_tag="AC1LW8_002560"
     CDS             2034..2654
                     /locus_tag="AC1LW8_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774315.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT         1068 a          424 c          513 g         1068 t
ORIGIN      
        1 ttcttcatta gtataaatat aattattaag aaaggtatat atctttgtaa ccttcgttta
       61 cattaataat gttgtgataa cctttgtgtt ctaaaatacc aatagctata gaacttctaa
      121 tgccagactg acagtctaca taaataacat cgtttctatt gaaaggtaaa tctgtttcta
      181 aaagtttgcc gtgtggcaca tgaaccgctt gggataagtg gccattattc cattcattat
      241 cattacgtac atctaagaca tgtgcttcat taccagttat gtctttacta tgaacagatt
      301 gtgtttgaat ttgagcttgt ggtaactgat atccagacac attatcatat ccaataagtt
      361 gtaaagtatg ttatgtgttg cttttgaaac aaggtgatag tctccaatca agttaatttc
      421 ttgattatag tttagatacc agccaatttg attgatgaaa tttttatcat atggaatatt
      481 gattgtacct tcaatatgtc caccatgata agcctcctta ctgcggagat caaaagttaa
      541 tctgtttgta cttgtagctg gataaaccgt ataaggttga tataaattca taccgaattg
      601 attaattttt ttcatttgtg caaaatgatg tggtggtgca ggttggtcag aaatgagttt
      661 atcgataaag gtagcttcat tattttcaga aaaagcccag ttcgtttgtt tttcatagcc
      721 aagcgtagat gttggaatag cacctaaaga tttaccacaa ggactaccag cgccatgacc
      781 aggccaaatt tgaatgtagt ctggcaagtc tttaatactt tcaatagatt taaacatttg
      841 ttttgcgcct atttcagata atccttctac tttaacagct ttttctagta aatcaggtct
      901 accgatatct cctacaaaaa taaaatcacc actgaatagt cccattggaa cttgtgctcc
      961 agcaccttcg tcagtaagta aaaaacttat actttctggc gtgtgaccag gtgtatgaag
     1021 cacttttaat tttatatttc ctacataaat atcatcatta tgttgaacaa aatgagtgtg
     1081 gttaggcata tttttataac ctaacatgtc atcactttcg cccgatacat aaatactagc
     1141 atttaacttt atagcaacat ctctaattcc tgaaacaaaa tttgcatgta tatgtgtttc
     1201 agctgcatga gtaatggtta aactctcttc atcggcaact cgaatatatg aagataagtc
     1261 acaaatagga tcaatgatca tggcttctcc agttttttga caaccgatta aataagatgc
     1321 ttgagataaa tgtttatcat aaattgatga tagtcaattt gttatcaagg gtgataatta
     1381 tattaactat ggtaattaca tggaattaaa gttattaaac aaacttgatg atgaccaatt
     1441 agatttagaa tttttacaag ataatgggtt aaaaggtaat gttcaaggtc ctatgacttt
     1501 aaaggaacct ttaaaaagtt atatacaaaa gtcttgaaaa cgaatatttt aaatttagat
     1561 aatgagttag aaaacactga atatgtcgaa ggtaaaccat atgtacagta cggtattaaa
     1621 tatgaaagaa atcaggcatt aagaaatgaa gctattaaaa ttcatggaac tacatgtaaa
     1681 gtatgtggat ttgattttaa agctaagtat ggcgatttag gtgagggttt tattgaaatt
     1741 catcatttaa aaccaatgtt ttcaataaaa agagaaataa aagtaaatcc acaaaaagat
     1801 ttagtcccac tatgttctaa ttgccataaa atgattcata gaaatactaa aaaaccttta
     1861 acgattaaag aattaaccaa aatagttaat tataatagca aataatttaa tattttataa
     1921 actatcattc aaccctctta atttattagg aggttttttg tatttatgct ttcaaatgtg
     1981 tgatatactt tgtttgttga aatatagagt atctatagat agggtgattg agtatgaaat
     2041 tcactgaagt ggaagttatc gaacatcttg taaaggcata taaagaagca ggaaagccta
     2101 cttatcctca tgaaaattta tatcgaggac gtaatcatag tatttcaggt attggagaag
     2161 acttgctggg tgcttatttg attagtagat tggaaggtgt ccaaatattt attgatcagc
     2221 ctttatctat gattgataaa tctttaagta caagatatcc ggatttatta atttgtgaag
     2281 ataatgaaat taaaaatata ctagaagtta aaatggactt aggatatcaa agaaaagatt
     2341 ttatagatta ttgccgaaag aaagaagaat ggatttcaaa tatcgtagga aaacagtgtg
     2401 tattgtctag aaagagagaa gacaaaattc ctatgaatat agctgatgat attaaatttc
     2461 atgttgtgat ttacagtgaa aacaatggac cgaagcggtt tgatgaagaa atcatgccta
     2521 tcgttaatga aacatgtcca catattgaag tatatgtcct aacaagcggt caacacccta
     2581 atttagtaaa tgttaatctt gaaggtatta atattaataa agatgaattt gaaatattag
     2641 taaatgcgtt ataaaaaaat agagcatcct ccacgttatg gaggtgctct gttttttatt
     2701 gaaaagtatc aagttaatta atttaatatg cttaataagt tctaccttga cctttttctc
     2761 tagcttctgt tcgatctctt atgtactcag tacatactgg attttctgtt aattcatcaa
     2821 ctgtattagt ttaatcagaa gacgtgtcta ctttgtaagc ttctaagaac ttattatatg
     2881 atatagcgtt tgagttttgt tgttcttcta taaatttctg tagttatttt tcaaaaaccg
     2941 catcattaac tgataagcag aggcgtatca taagtaatga ggttcatgat ttttgacata
     3001 gttagcctcc gcagtctttc aagtaaataa tatcgaaata ttctttatag tgaatattta
     3061 tagtgaagca aag
//
LOCUS       DBNENW010000205         3043 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNENW010000205 DBNENW010000000
VERSION     DBNENW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.209"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            29..661
                     /locus_tag="AC1LW8_002561"
     CDS             29..661
                     /locus_tag="AC1LW8_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774316.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            686..1855
                     /locus_tag="AC1LW8_002562"
     CDS             686..1855
                     /locus_tag="AC1LW8_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HIP4774317.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENSQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(1953..2942)
                     /gene="ccpA"
                     /locus_tag="AC1LW8_002563"
     CDS             complement(1953..2942)
                     /gene="ccpA"
                     /locus_tag="AC1LW8_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HIP4774318.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
BASE COUNT          985 a          529 c          493 g         1036 t
ORIGIN      
        1 tcacgacgtc gattgaggag ttgaaaatat gaatcattta aagacgtatc aatccgaaga
       61 ttattacatt catgacaagc aatttgttat tgaaggtcct ttaacatacg aagatttgaa
      121 agcgcttact ttcgatgcgc atttaaccgc atttagagat gctgaagatc agtatgaagc
      181 tttgttagaa attacaacat taccagaagg tagaatttat gttgctcgcc aagatcaact
      241 cattgtgggt tatgtcactt tccactatcc tgatgaaatt gagcgctggt ctacaggtaa
      301 ccttccatat ttaatcgaat tgggggcaat tgaagtcagc atcaatttta ggcaattaca
      361 tcttgcagaa aagctaatac aacttagcct ttctacacca gaattcgagg attatatcgt
      421 tataactact gaatattact ggcattggga tttaaaaaat tcaaagttag atgtatttga
      481 ctataaaaaa ttaatgcagc ggttaatggc aactggtgga cttgaaatat tcgctacaga
      541 tgatccagaa ataacaagtc atccagctaa ttgtttaatg gcaagaattg gcaaaaatat
      601 tacattagaa cagcaacaag cgtttgatga tattcgttat atgaatcggt ttttctttta
      661 atttttggaa tatcggaggg attatatgca acaacattca tcaaaaactg catatgttta
      721 ttcagataag ttattacaat atcgatttca tgaccaacat cccttcaatc aaatgcgttt
      781 aaaattaaca acagagctac ttttgaatgc aaatttattg tctccagaac aaatagtaca
      841 acctagaatt gcaacagatg acgaattaat gttaattcat aaatatgatt acgtcgaagc
      901 aattaagcat gcttcacatg gcattatcag tgaagatgag gctaagaaat atggattaaa
      961 tgatgaagag aatagtcaat ttaagcatat gcaccgccat agtgccacaa ttgttggagg
     1021 cgctttaact ttagcagatc ttattatgtc aggcaaagta ttaaatggtt gtcacttagg
     1081 tggtggattg catcacgctc aacctggtcg agctagtggt ttttgtatat acaatgatat
     1141 tgcaattacc gcacaatact tagctaaaga atacaatcaa cgcgttttaa tcatagatac
     1201 cgatgcacat catggagatg gtacacaatg gagtttctat gccgataacc atgttactac
     1261 ttattctatc catgaaaccg gaaaatttct tttcccaggc tctggtcact atactgagcg
     1321 cggtgaagat atcggctatg gacacactgt aaatgtccca cttgaaccgt atacagaaga
     1381 tgcatcattt ttggagtgtt ttaaattaac agttgagcct gtcgtaaaga gttttaaacc
     1441 tgatattatt ctaagcgtaa atggtgtcga tatacattat cgtgatccac taactcatct
     1501 aaattgtacg ttacattcat tatatgaaat tccatatttt gtaaaatatt tagctgattc
     1561 ttatacgaat ggaaaggtaa ttatgtttgg tggcggaggc tacaatattt ggagagtcgt
     1621 accacgtgca tggagtcatg tattcttaag tttaattgat caaccaattc aaagtggtta
     1681 tttaccgtta gaatggatta ataaatggaa acattattca tctgaattat tacctaaaag
     1741 atgggaagat cgtttaaatg attataccta tgtcccccgc acaaaagaaa ttagtgaaaa
     1801 aaataaaaaa ttagctttac atatagcgag ttggtacgaa tctactcgtc aataagtaac
     1861 ctaatctgca acgattttaa aagataaata aaaggcattc ttccaacacc catataattt
     1921 gggtcgttag atgaatgcct aattttgtga atttattttg tagttcctcg gtattcaatt
     1981 ctgtgaggta aaactacatt tggttcttct atcttttcat cgttcatata ttttgttaat
     2041 aagcgcatcc ctactgcacc gatatcatat aatggttgaa taacactaga aagttgtggt
     2101 ctaaccatct caactaatcg tgtattattg aaactaataa tttgtaattc ctctggaact
     2161 ttaataccag catccattgc actatgcata ataccaattg cttcttcgtc gctgatacat
     2221 aaaatggcat ctggcaaatt gcctttcatt ttggcaaaag cttttacgcc ttctttataa
     2281 ctttcagcac cagaacaatt caatgtatca cctaattgaa ggccattttt atttaacact
     2341 tcagttaaac cttctaaaac atcttcttga gcttttttag aatgttctcc acctactaaa
     2401 gcaaatgatt tagcgccttt ttcaattaat tctcccgtaa tttctttcgc agcttcagta
     2461 aaatcaatat taactgatgc tatatgtgca tccttaccat ttgttcctga tactactaca
     2521 ggtacagatg attgatttat caattctttc atttcttcag taattgtacc accaaggaaa
     2581 ataataccat caacctgttt acttaataag ttattaaaaa tttctttttc cttttcagga
     2641 tcgttatctg aatttgaaat aattgagtga tatttataca ttgttgcaat atcttcaagt
     2701 ccacgagcaa gttgtgaata atagatatta gatatatctg gaatgatcac acctactgtt
     2761 gttgtctttt tactagctaa acctctagca acagcatttg gacgataatt caaacgctta
     2821 atgacttcgt taactttatt tttagtttct gctttaacat tttggttccc attaacaaca
     2881 cgcgacactg tggccataga gacacgcgct tctcttgcta catcatatat agtaactgtc
     2941 ataatttcct ccttgtaaac gttttattca ttataataac atgttttcat ttcattttca
     3001 tagtttacca tattttaatt tacagtaaca tatttttgtt tgg
//
LOCUS       DBNENW010000206         2969 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.210, whole genome shotgun sequence.
ACCESSION   DBNENW010000206 DBNENW010000000
VERSION     DBNENW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.210"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            43..1029
                     /locus_tag="AC1LW8_002564"
     CDS             43..1029
                     /locus_tag="AC1LW8_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HIP4774319.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYLNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            1250..2020
                     /locus_tag="AC1LW8_002565"
     CDS             1250..2020
                     /locus_tag="AC1LW8_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774320.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNNKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            2072..2842
                     /locus_tag="AC1LW8_002566"
     CDS             2072..2842
                     /locus_tag="AC1LW8_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774321.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLKPSEDK"
BASE COUNT         1220 a          320 c          557 g          872 t
ORIGIN      
        1 gatgtataat caatgtatta aatatagaaa gaagttgata ttatgaaaaa gtgtattaag
       61 actttgtttt taagtatcat tttagtagtg atgagtggtt ggtatcattc agcacatgcg
      121 tcagattcgt tgagtaaaag tccagaaaat tggatgagta aacttgatga tggaaaacat
      181 ttaactgaga ttaatatacc gggttcacat gatagtggct cattcacttt aaaggatcca
      241 gtaaaatcag tttgggcaaa gactcaagat aaagattacc ttacccaaat gaagtcggga
      301 gtcaggtttt ttgatattag aggtagagca agtgctgata atatgatttc agttcatcac
      361 ggcatggttt atttgcatca tgaattagga aaatttctcg atgatgctaa atattacttg
      421 agtgcttatc caaacgaaac aattgtgatg tctatgaaaa aggactacga tagcgattct
      481 aaagttacga agacatttga agaaattttt agagaatatt attataataa cccgcaatat
      541 cagaatcttt tttacacagg aagtaatgcg aatcctactt taaaagaaac gaaaggtaaa
      601 attgtcctat tcaatagaat ggggggtacg tacataaaaa gtggttatgg tgctgacacg
      661 tcaggtattc aatgggcaga caatgcgaca tttgaaacga aaattaataa tggtagctta
      721 aatttaaaag tacaagatga gtataaagat tactatgata aaaaagttga agctgttaaa
      781 aatttattgg ctaaagctaa aacggatagt aacaaagaca atgtatattt gaatttcttg
      841 agtgtagcgt ctggaggcag cgcatttaat agtacttata actatgcatc acatataaat
      901 cctgaaattg caaaaacgat taaagcaaat gggaaagcta gaacgggttg gctgattgtt
      961 gactatgcag gatatacgtg gcctggatat gatgatatcg taagtgaaat tatagatagt
     1021 aataaataag gattcaataa tgatattaag acgagtatga aaatagttag attctaatta
     1081 ttttcactac tcgtttttat tttgaaaata agtaataatt caacaatatt ataaattgaa
     1141 cagattgttt gtgaaatttt tgataatatt aaagtgaaaa agtgttataa attgataaat
     1201 atatgtaatt aacaaaaaca aatcatttta aaaagaagag agttgtaaga tgatgaaacg
     1261 attaaacaaa ttagtgttag gcattatttt tctgttttta gtcattagta tcactgctgg
     1321 ttgtggcata ggtaaagaag cggaagttaa gaaaagcttt gaaaaaacat tgagtatgta
     1381 ccctattaaa aatctagagg atttatacga taaggaaggc tatcgtgatg atcagtttga
     1441 taaaaatgat aaaggtacat ggattataaa ttctgaaatg gttattcaac ctaataatga
     1501 agatatggta gctaaaggca tggttctata tatgaataga aataacaaaa caacaaatgg
     1561 ttactactat gtcgatgtga ctaaggacga ggatgaagga aaaccgcacg acaatgaaaa
     1621 aagatatccg gttaaaatgg tcgataataa aatcattcca acaaaagaaa ttaaagatga
     1681 aaaaataaaa aaagaaatcg aaaactttaa gttctttgtt caatatggcg actttaaaaa
     1741 tttgaaaaat tataaagacg gagatatttc atataatcca gaggtgccga gttattcggc
     1801 taaatatcaa ttaactaatg atgattataa tgtaaaacaa ttacgcaaaa gatatgatat
     1861 accgacgagt aaagctccaa agttattgtt aaaaggttca gggaatttaa aaggctcatc
     1921 agttggatat aaagatattg aatttacgtt tgtagagaaa aaagaagaaa atatatactt
     1981 tagtgatagc ttagattata aaaaaagcgg agatgtataa tcatggctca atcagaatat
     2041 gaaatcaatc ccggaaaaag agagtgatga aatgataaaa cgtgtaaata aattagtgct
     2101 tggtattagt cttctgtttt tagtcattag tatcactgct ggttgtggca tgggtaaaga
     2161 agcggaaata aagaaaagtt ttgaaaaaac attgagtatg tatccgatta aaaatctaga
     2221 ggatttatac gataaagaag gatatcgtga tgatcaattt gataaaaatg ataagggaac
     2281 atggattgta aattctcaaa tggcaattca aaataaagga gaagctctaa aaataaaagg
     2341 catgcttttg aagatagata gaaatacaag aagtgcaaaa ggattttact atactaatga
     2401 aataaagacg gagaaatacg aagtagctca ggataatcaa aaaaaatatc cagttaaaat
     2461 gattaataat aaattcattt ctactgagga agttaaagaa gaaaacataa aaaaagaaat
     2521 cgaaaacttt aagttttttg cgcaatatag caattttaaa gatttaatga attataaaga
     2581 tggagatata tcatataatc cagaggtgcc gagttattca gctcaatatc aattaactaa
     2641 tgatgattat aatgtaaaac aattacgtaa aagatatgac ataccaacaa ataaagcgcc
     2701 gaagctgttg ttgaaaggta cagggaattt aaaaggttca tcagttggat ataaaaaaat
     2761 tgaatttact tttttagaga ataaaaatga aaatatttac tttactgata gtctacatct
     2821 taaaccgagc gaggataaat aaacgtgacc aataaagagt atgaaatcga acccggaaaa
     2881 ataacaagta attttgtaga aactagcgca acatcaaata tatgagaata gtcaaattat
     2941 aagtaattaa caacgtatgt tatttcaaa
//
LOCUS       DBNENW010000207         2966 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNENW010000207 DBNENW010000000
VERSION     DBNENW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.211"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..499)
                     /locus_tag="AC1LW8_002567"
     CDS             complement(<1..499)
                     /locus_tag="AC1LW8_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4774322.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVL"
     gene            609..1754
                     /locus_tag="AC1LW8_002568"
     CDS             609..1754
                     /locus_tag="AC1LW8_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIP4774323.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            1738..2379
                     /locus_tag="AC1LW8_002569"
     CDS             1738..2379
                     /locus_tag="AC1LW8_002569"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP4774324.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            2372..>2966
                     /locus_tag="AC1LW8_002570"
     CDS             2372..>2966
                     /locus_tag="AC1LW8_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774325.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEE"
BASE COUNT         1180 a          332 c          453 g         1001 t
ORIGIN      
        1 tcaatacaat tttaccttcg catacttttt cagaatgaac ttctcttagt ttaggttgat
       61 ttctgctaat cccgatgtca tacgtatgat tattaatatc tttttcaata ttttcatttt
      121 tgacatgaag agaaacatcg ataaaaggat gctcgttaaa gaaagatttt aaaaatttgg
      181 gcataatgaa tgtcgcgata tatgaagaca cgacaacatt taatttcgat tgaaacatcg
      241 tttttttaag ttggatatgt ttgataccac tttcatattg ttcaataaaa ctttgcgcaa
      301 ttggaagaaa tgtatgacca tcttcagtca agataatttg atttttataa gtttcaaata
      361 gtttcacatt gagatgctgt tctaaatttt taatttgctt atgtatagaa ggtatagtga
      421 gattaatttc ttcactagct aatcgatagt ttaacgtctt cgctaaaatg acaaatgtat
      481 agtaccaatc taaattcatg aacttacctc tttcaaaaaa gttaataatt cattagataa
      541 tatttaattt ttattgcata aaaataatat taatattata tcagaggtta tcgagggggt
      601 gaagtaatat gattcgaatt caagataata caataaggga tggtatgcaa caaagtaatg
      661 ttcgaaaaag tctaattata aaaaaagaag tattgaaaca aattaacaag ttaaatataa
      721 attctgttga agtaggcatg tgtacaacta tcgaggatga atttaatatt catcaattca
      781 gagacatttt aagtcctgaa aaagaattag tagtattgac taggcttaat gaaaaagaaa
      841 taaaaaaaat agtcaaattg aaaattcata atttagtggt aaaaatacta ttgccaatat
      901 ctgacttgca tataaaagaa aagcttaatt tttcaaataa atattatatt cagaaaatca
      961 aagactgctt ggatatatta aagaaagata aaaaaggagt agatatttgt tttgaagatg
     1021 caacaaggac ttctagagaa aaattgaaag aatacatgga aattatttca aaatatcaag
     1081 ttagaacagt tacatttgcg gacactgtag gatgttcgac accattagaa tacggagata
     1141 tttttaatta ctttgtaaaa aaatattcta acataatttt ttctgctcat tgtcataacg
     1201 atctagggtt ggctactgca aatacattag ctgcaatttt aaatggtgca aagcaaatag
     1261 aaactacatt tttgggaatt ggtgagagag cgggtaatgc tcctattgag gaaataatta
     1321 ctattttgac aaaaaaacaa atagaaagta cggaattcac tttacccgac gtatataaaa
     1381 ctagtattaa tatttctaaa attctcgatt ttcaaatatc agaaaacaaa cctataattg
     1441 gtgaaaatat atttaaacat gaatcaggaa ttcatcaaga tggtactaaa aaaaatataa
     1501 atatgtatca atatttagtt cctagtgatt taggattgaa aaattcacaa gttgttcagt
     1561 ttccaataag taatatttct agtaagaaaa tcttgcacaa taaatttaaa tcaatagtta
     1621 acactgaaga aattgatgaa aatatttctt tctataaact tgtgaacaag ttttcacctg
     1681 aagtagcacc tgaagataca gtggatttac ttcagataat aaaaaggagg agtaaagatg
     1741 gaaattttaa atgattctgt aaaaagtttt gaaaaacttt atttacaatt acaagatggt
     1801 tttccagtga ttgttcctac tgatactaat tataatttat gtagtttacc taacaacgat
     1861 ctttgtatcg ataaaatatt tgaatataaa aagcgatcga aagataagcc attatcatta
     1921 tttattgata agccagagga ttggaaattg tatggagata atcaaaatac ggaaatagtt
     1981 gataaactag ttgaaatatt ttggcctgga ccattaaata ttattttaaa aaataaaaca
     2041 agctataatt atatgctcaa taattcagat agtatagcta taggatgtgt acaaaataaa
     2101 acgatgagaa gatttatttc atatattaat tcacctatag caattacttc agcaaatata
     2161 tctggaactg ccgatgatat tttaataact gaaaatgaag caattaagca catgggcgaa
     2221 aatgtaagat atatgctaag aagtcaaaat aaaactaact ataaaacatc tagtacgatt
     2281 attaaagtga cagataataa aattgaatta ttaagagaag gagatataaa gtttgaagaa
     2341 ataaaagaaa gactaggtac aggtattatt tatgaataag ttaatacttg ggatttattt
     2401 ataccgaatt ttttcacgag catactttta tttaccgttt ttattaattt actttttgat
     2461 tcaaggttat tccataatac aattagaaat attaatggcg tcttatggca ttgcagcatt
     2521 tttattctct ctatacaaag agaagtgttt taaaatttgt aacttaaaag attctaataa
     2581 attagttgtt agtgaaatat tcaaaatcat cggtttattg ttgttattat atcaaaatca
     2641 atatttaatt ttagtagtgg cacaaatatt attagggtta agttactcaa tgatggcggg
     2701 tgttgatacc gcaataatta aaagaaatat aacaaatgag aaatacgtac aaaataagtc
     2761 aaatagctat atgttcctat cattattaat ttcagggatt ataggtagtt atctttatgg
     2821 aataaatatt aaatggccta taataatgac tggtatattt tcaattctaa caattataat
     2881 tattcgatgc acattagttg aaaataggga attaaattta ataggagaaa caaagggaaa
     2941 gataaagaaa tttctaccag aagaga
//
LOCUS       DBNENW010000208         2960 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.212, whole genome shotgun sequence.
ACCESSION   DBNENW010000208 DBNENW010000000
VERSION     DBNENW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.212"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            35..2848
                     /gene="sdrC"
                     /locus_tag="AC1LW8_002571"
     CDS             35..2848
                     /gene="sdrC"
                     /locus_tag="AC1LW8_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HIP4774326.1"
                     /translation="MNNKKTVTNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTEKVDSCQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKDVSATPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHLNKDTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVSLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTNQFNITYSNDNKTATVDLMNGQTSSNKQYIIQQ
                     VAYPDNTSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANAGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDTDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSESDADSDTDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNSNNGT
                     LFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1176 a          484 c          538 g          762 t
ORIGIN      
        1 atcaaatttt gatattttaa aggagtatta ttaaatgaat aataaaaaga cagtaacaaa
       61 tagaaaaggc atgataccaa atcgattaaa caaattttcg ataagaaagt attctgtagg
      121 tactgcttca attttagtag ggacaacatt gatttttggg ttaagtggtc atgaagctaa
      181 agcggcagaa catacgaatg gagaattaaa tcaatcaaaa aatgaaacga cagccccaag
      241 tgagaataaa acaactgaaa aagttgatag ttgtcaacta aaagacaata cgcaaactgc
      301 aactgcagat cagcctaaag tgacaatgag tgatagtgca acagttaaag aaactagtag
      361 taacatgcaa tcaccacaaa acgctacagc tagtcaatct actacacaaa ctagcaatgt
      421 aacaacaaat gataaatcat caactacata tagtaatgaa actgataaaa gtaatttaac
      481 acaggctaaa gatgtttcag ctactcctaa aacaacgact attaaaccaa gaacattaaa
      541 ccgtatggct gtgaacacag tagcagcacc acaacaaggt acaaatgtta atgataaagt
      601 tcatttttca aatattgata ttgctattga taaaggacac ttaaataaag atactggcaa
      661 aacagaattt tgggcaactt caagtgatgt tttaaaatta aaagcgaatt acacaatcga
      721 tgattctgtt aaagagggcg atacatttac ttttaaatat ggtcaatatt tccgtccagg
      781 atcagtaaga ttaccttcac aaactcaaaa tttatataat gcccaaggta atattattgc
      841 aaaaggtatt tatgacagta caacaaacac aacaacgtat acttttacga attatgtaga
      901 tcaatataca aatgttagcg gtagctttga acaagtcgca tttgcgaaac gtgaaaatgc
      961 aacaactgat aaaactgctt ataaaatgga agtaagttta ggtaatgata catatagcga
     1021 agaaatcatt gtcgattatg gtaataaaaa agcacaaccg cttatttcaa gtacaaacta
     1081 tatcaacaat gaagatttat cgcgtaatat gactgcatat gtaaatcaac ctaaaaatac
     1141 atatactaaa caaacgtttg ttactaattt aactggatat aaatttaatc caaatgcaaa
     1201 aaacttcaaa atttacgaag tgacagatca aaatcaattt gtcgacagtt tcaccccaga
     1261 tacttcaaaa cttaaagatg ttactaatca atttaatatt acttacagca atgataataa
     1321 gactgcaaca gttgatttaa tgaatggtca aacaagtagt aataaacaat acatcattca
     1381 acaagttgcg tatccagata atacatcaac agataatgga aaaattgatt atactttaga
     1441 cactgacaaa actaaatata gttggtcaaa tagttattca aatgtgaatg gctcatcaac
     1501 tgctaatggc gaccaaaaga aatataatct aggtgactat gtatgggaag atacaaataa
     1561 agatggtaaa caagatgcca atgaaaaagg gattaaaggt gtttatgtca ttcttaaaga
     1621 tagtaacggt aaagaattag atcgtacgac aacagatgaa aatggtaaat atcagttcac
     1681 tggtttaagc aatggaactt atagtgtaga gttttcaaca ccagccggtt atacaccgac
     1741 aactgcaaat gcaggtacag atgatgctgt agattctgat ggactaacta caacaggtgt
     1801 cattaaagac gctgacaata tgacattaga tagtggattc tacaaaacgc caaaatatag
     1861 tttaggtgat tatgtttggt acgacagtaa taaagatggt aaacaagatt cgactgaaaa
     1921 aggaattaaa ggtgttaaag ttactttgca aaacgaaaaa ggcgaagtaa ttggtacaac
     1981 tgaaacagat gaaaatggta aataccgctt tgataattta gatagtggta aatacaaagt
     2041 tatctttgaa aagcctgctg gtttaactca aacaggtaca aatacaactg aagatgataa
     2101 agatgccgat ggtggcgaag ttgatgtaac aattacggat catgatgatt tcacacttga
     2161 taatggctac tacgaagaag aaacatcaga tagtgactca gattcagaca gcgactcaga
     2221 ctcagatagc gactcagatt cggacagcga ttcagactca gacagcgact cagactcaga
     2281 cagtgattca gattcagaca gcgattcaga ctcagatagc gactcagatt cggacagcga
     2341 ttcagactca gatagcgact cagattcaga cagcgactca gactcagaca gtgattcaga
     2401 ttcagacagc gactcagatt cagatagcga ctcagactca gacagtgatt cagattcaga
     2461 cagcgattca gatacagata gcgattcaga ctcggatagc gactcagact cggatagcga
     2521 ttcagactca gatagcgatt cagactcaga ctcggatagc gattcagatt cagacagcga
     2581 ctcagattcg gacagcgatt cagactcaga tagcgactca gactcagaaa gtgatgccga
     2641 ttcagataca gatagcgatt cagatgcagg taaacatact ccggctaaac caatgagtac
     2701 ggttaaagat cagcataaaa cagctaaagc attaccagaa acaggtagtg aaaataataa
     2761 ttcaaataat ggcacattat tcggtggatt attcgcggca ttaggatcat tattgttatt
     2821 cggtcgtcgt aaaaaacaaa ataaataata tgattaactt aaccaggtcc atgtggcctg
     2881 gttttttctg tttagaaatt cccagatata aaacaaaaaa cctttaagga atagatacaa
     2941 tttaatttgt tacagaaatg
//
LOCUS       DBNENW010000209         2918 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNENW010000209 DBNENW010000000
VERSION     DBNENW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.213"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            283..747
                     /gene="accB"
                     /locus_tag="AC1LW8_002572"
     CDS             283..747
                     /gene="accB"
                     /locus_tag="AC1LW8_002572"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIP4774327.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            747..2102
                     /gene="accC"
                     /locus_tag="AC1LW8_002573"
     CDS             747..2102
                     /gene="accC"
                     /locus_tag="AC1LW8_002573"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIP4774328.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2117..2479
                     /locus_tag="AC1LW8_002574"
     CDS             2117..2479
                     /locus_tag="AC1LW8_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP4774329.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2539..>2918
                     /gene="nusB"
                     /locus_tag="AC1LW8_002575"
     CDS             2539..>2918
                     /gene="nusB"
                     /locus_tag="AC1LW8_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIP4774330.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSN"
BASE COUNT         1089 a          411 c          559 g          859 t
ORIGIN      
        1 taaaggtata tatctacata tcatattgtt gaggattaaa cataatagct aataaaaata
       61 acattgatat gtttaactga atgcataaaa tttttaatca agagttaaga tgagtcaatg
      121 ttaagtaata aatacacaag tgtttttaat ttaaaatgtg tttttattat atctttggga
      181 tagacttata atgtcgccag caatatgaac atgcttgaat tgaagagttg tctcaagtaa
      241 aatagacggg tagatgaaaa caaactgaag gagtcagtaa taatgaactt taaagaaatc
      301 aaagaattaa ttgaaattct ggataaatca actttaacgg aaatcaatat tgaagatact
      361 aaaggcaaag tgacgcttaa gaaagaaaaa gaaactgaga ttatcacgcc acaaatctca
      421 caaatgccag ttgaagctgc ggcaatgcct atgcctcaag tacaatcaac tgatagcaat
      481 aaaactgaag ctccaaagcc aacttcagat aatcacaaaa caattaatgc acctatggta
      541 ggtacatttt acaaatcgcc atctccagac gaagaagcat atgtgcaagt tggggacact
      601 gtttcaaatg aaacaacagt gtgtatttta gaggcaatga aactatttaa tgaaattcaa
      661 gcagaaattt caggtgaaat tgttgaaatc ttagtagaag acggacaaat ggtagagtat
      721 ggccaaccgt tatttaaggt gaaataatga aaaaggtttt aattgcaaac cgcggtgaaa
      781 tcgcagttag gattattcgc gcttgtcgtg atttaggcat ccaaactgtt gcaatctatt
      841 ctgaagggga taaagatgcg ctacatactc aaattgctga tgaagcatat tgcgtaggtc
      901 ccactttgtc taaagattca tatttaaata ttccgaacat cttatctatt gcaacttcta
      961 caggttgtga tggcgttcat ccgggttatg gctttttagc tgaaaatgct gactttgcag
     1021 aattatgcga agcatgccaa ttgaagttca ttggaccaag ttatcaatct atccaaaaaa
     1081 tgggtatcaa agatgttgct aaggcagaaa tgatcaaagc caatgttcca gttgttcctg
     1141 gtagtgatgg tttaatgaaa gacgtctcag aagctaagaa aatcgctaaa aaaattggct
     1201 atccggtcat cattaaagct actgctggtg gtggcggaaa aggtattcgt gttgctcgtg
     1261 atgaaaaaga acttgaaact ggcttccgaa tgacagaaca agaagctcaa actgcatttg
     1321 gtaatggtgg actttatatg gagaaattca tcgaaaactt ccgccatatt gaaatccaaa
     1381 ttgttgggga cagctatggt aatgtaattc atttaggaga acgtgattgt acaattcaaa
     1441 gacgtatgca gaaattagtg gaagaagcac cttccccaat tttagatgat gaaacacgtc
     1501 gtgaaatggg aaatgccgca gtgcgtgcag cgaaagctgt aaattatgaa aatgcgggaa
     1561 caattgagtt tatatatgat ttaaatgata ataaatttta ttttatggaa atgaatacac
     1621 gtattcaagt agaacatcct gtaactgaaa tggtaacagg aattgattta gttaaattac
     1681 aattacaggt tgctatgggt gacgtgttac cgtataaaca agaagatatt aaattaacag
     1741 gacacgcaat tgaatttaga attaatgctg aaaatcctta caagaacttt atgccatcac
     1801 caggtaaaat tgagcaatat cttgcaccag gtggatatgg tgttcgaata gagtcagcat
     1861 gttatactaa ttatacgata ccgccatatt atgattcgat ggtagcgaaa ttaatcatac
     1921 atgaaccgac acgagatgaa gcgattatgg ctggcattcg tgcattaagt gaatttgtgg
     1981 ttcttggtat tgatacaact attccattcc atattaaatt attgaataac gatatattta
     2041 gaagcggtaa atttaataca aactttttag agcaaaatag cattatgaat gatgaaggtt
     2101 aataggaggt caatccatgg tcaaagtaac tgattattca aattcaaaat taggtaaagt
     2161 agaaatagcg ccagaagtgc tatctgttat tgcaagtata gctacttcgg aagtcgaagg
     2221 catcactggc cattttgctg aattaaaaga aacaaattta gaaaaagtta gtcgtaaaaa
     2281 tttaagccgt gatttaaaaa tcgagagtaa agaagatggc atatatatag atgtatattg
     2341 tgcattaaaa catggtgtta atatttcaaa aactgcaaac aaaattcaaa cgtcaatttt
     2401 taattcaatt tctaatatga cagcgataga acctaagcaa attaatattc acattacaca
     2461 aatcgttatt gaaaagtaat gtcataccta attcagtaat taaataaaga aaaatacaaa
     2521 cgtttgaagg agttaaaaat gagtcgtaaa gaatcccgag tgcaagcttt tcaaacttta
     2581 tttcaattag aaatgaagga cagtgattta acgataaatg aagcgataag ctttattaaa
     2641 gacgataatc cagatttaga cttcgaattt attcattggc tagtttctgg cgttaaagat
     2701 cacgaacctg tattagacga gacaattagt ccttatttaa aagattggac tattgcacgt
     2761 ttattaaaaa cggatcgtat tattttaaga atggcaacat atgaaatatt acacagtgat
     2821 acacctgcta aagtcgtaat gaatgaagca gttgaattaa caaaacaatt tagtgatgat
     2881 gatcattata aatttataaa tggtgtattg agtaatat
//
LOCUS       DBNENW010000210         2895 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNENW010000210 DBNENW010000000
VERSION     DBNENW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.214"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            154..2763
                     /gene="adhE"
                     /locus_tag="AC1LW8_002576"
                     /gene_synonym="adhC"
     CDS             154..2763
                     /gene="adhE"
                     /locus_tag="AC1LW8_002576"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIP4774331.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT         1058 a          457 c          559 g          821 t
ORIGIN      
        1 atgatttcat gaaaacattt attttaaaat ttgatatttg ttcaaataat attcgaaatt
       61 aaactttttt gcatagaatt ttctttatat cctgagagac atgtactata atgtttgtga
      121 aataattcac aaagtataaa ggagtggttg tatatgttaa ctatacctga aaaagaaaat
      181 cgtggatcga aagaacaaga agtggcaatt atgattgatg ctctagctga caaagggaaa
      241 aaagcattag aagcattatc taaaaagtca caagaagaaa ttgatcatat tgttcatcaa
      301 atgagcttag cagctgttga tcaacatatg gtgctagcaa aattagcaca tgaagaaact
      361 ggaagaggta tatacgaaga taaagcgatt aaaaatttat acgcttctga atatatatgg
      421 aattcaataa aagacaataa gacagtaggg attattggtg aagataaaga aaaaggatta
      481 acgtatgtag cggaaccaat tggtgttatt tgtggtgtta cgccaacaac aaatcctacg
      541 tcgacaacta tttttaaagc gatgattgca attaagacag gaaatccaat catttttgca
      601 ttccatccaa gtgcacaaga atcgtcgaag cgtgcagcag aagttgtatt agaagcggca
      661 atgaaggcag gtgcacctaa agatattatt cagtggattg aagtgccttc tatcgaagca
      721 acaaaacaat taatgaatca caaaggtatt gcattagttc tagcaacagg tggttcgggc
      781 atggttaagt ctgcatattc aactggcaaa ccggcattag gtgtgggacc aggtaacgtg
      841 ccgtcttaca ttgaaaaaac agcacacatt aaacgtgcag taaatgatat cattggttca
      901 aaaacatttg ataatggtat gatttgtgct tctgaacaag ttgtagtcat tgataaagaa
      961 atttataaag atgttactaa tgaatttaaa gcacatcaag catactttgt taaaaaagat
     1021 gaattacaac gcttagaaaa tgcaattatg aatgaacaaa aaacaggtat taagcctgat
     1081 attgtcggta aatctgcagt tgaaatagct gaattagcag gtatacctgt ccccgaaaat
     1141 acaaaactta tcatagccga aattagcggt gtaggttcag actatccgtt atctcgtgaa
     1201 aaattatctc cagtattagc cttagtaaaa gcccaatcta caaaacaagc atttcaaatt
     1261 tgtgaagaca cactacattt tggtggatta ggacacacag ccgttatcca tacagaagat
     1321 gaaacattac aaaaagattt tggactaaga atgaaagctt gtcgtgtact tgtaaataca
     1381 ccatcagcgg ttggaggtat tggtgatatg tataacgaat tgattccgtc tttaacatta
     1441 ggttgtggtt cgtacggtag aaactcaatt tcacataatg ttagtgcgac agatttatta
     1501 aacattaaaa cgattgctaa acgacgtaat aatactcaaa ttttcaaggt gcctgctcaa
     1561 atttattttg aagaaaatgc aatcatgagt ctaacaacaa tggacaagat tgaaaaagtg
     1621 atgattgtct gtgaccctgg tatggtagaa ttcggttata caaaaacagt tgagaatgta
     1681 ttaagacaaa gaacggaaca gcctcaaatt aaaatattta gcgaagtcga accgaaccca
     1741 tcaactaata cagtatataa aggtctggaa atgatggttg atttccaacc ggatacaatc
     1801 attgcacttg gtggtggttc agcgatggat gctgcaaaag caatgtggat gttctttgaa
     1861 caccctgaga catcattctt cggtgctaaa caaaagttcc tagacatcgg taaacgtact
     1921 tataaaatag gcatgcctga aaatgcgacg ttcatttgta tccctacgac atcaggtaca
     1981 ggttcagaag taacaccatt tgcagttatc acagatagtg aaacaaatgt aaaatatccg
     2041 ttggctgatt ttgctttaac acctgacgtt gcaattattg accctcaatt tgtgatgagt
     2101 gtgccaaaaa gcgttacagc agatacagga atggatgtac taacgcatgc aatggaatca
     2161 tatgtatctg taatggcttc agactacaca agaggtttga gtctacaagc gattaaattg
     2221 acgttcgaat atttaaaatc atctgttgaa aagggtgata aagtttcaag agagaaaatg
     2281 cataacgcat caactttggc tggtatggca tttgcaaatg cattcttagg cattgcacac
     2341 tcaattgcgc ataaaattgg tggcgaatat ggtattccgc atggtagagc gaatgcgata
     2401 ttactaccgc atattatccg ttataatgcc aaagacccgc aaaaacatgc gttattccct
     2461 aaatatgagt tcttcagagc agatacagat tatgcagata ttgccaaatt cttaggatta
     2521 aaaggtaata cgacagaagc acttgtagaa tcattagcta aagctgtcta cgaattaggt
     2581 caatcagtcg gaattgaaat gaatttgaaa tcacaaggtg tgtctgaaga agaattaaat
     2641 gagtcaattg atagaatggc agagctcgca tttgaagatc aatgtacaac tgctaatcct
     2701 aaagaagcac taatcagtga aatcaaagat atcattcaaa catcatatga ttataagcaa
     2761 taatctatct gataataatc atctaactca cctgaaatta caaaagtaaa aaatgccaca
     2821 taaactttaa gtcgataatc attatacggt tatcggcttt tatttattgc caaatcttca
     2881 gagagataca aacta
//
LOCUS       DBNENW010000211         2880 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNENW010000211 DBNENW010000000
VERSION     DBNENW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.215"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(188..760)
                     /gene="hpf"
                     /locus_tag="AC1LW8_002577"
     CDS             complement(188..760)
                     /gene="hpf"
                     /locus_tag="AC1LW8_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIP4774332.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(821..1496)
                     /locus_tag="AC1LW8_002578"
                     /pseudo
     CDS             complement(821..1496)
                     /locus_tag="AC1LW8_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            complement(1489..2571)
                     /locus_tag="AC1LW8_002579"
     CDS             complement(1489..2571)
                     /locus_tag="AC1LW8_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIP4774333.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
BASE COUNT          895 a          499 c          401 g         1085 t
ORIGIN      
        1 actcggacta tacaaaagac tgacatacta ttattaactt aacattcaaa tatatacatc
       61 cattaacatt agcatagtca ctatgtttca ttcaacaaat tacattatcg aactatgaaa
      121 tagtcataat ttgcttttgg agtataaaaa agcacttgtg caaaaacaca agtgctttaa
      181 acttaattta ttgttcacta gtttgaatca agccatattt accgtcttta cggcggtaaa
      241 cgatacttgt tccatcagtt tctctgtctg tgaatacaaa gaagtcatga cctaatagat
      301 tcatttgtaa taccgcttct tctgaatcca ttggttttaa gctgaattct tttgaacgaa
      361 taatttctat ctcgttatca tcgtaagcgt cattatcaac ttgtgtttct tgcatttctt
      421 gtaattcggc aacaaacact tcttgatctc ctcgatcacg gctcttacga ttaatacgtg
      481 ttttatattt tcgaacttgt ctttcaagtt tattattaat taaatcaata cctgcgtata
      541 aatcatcgtt tcgctcttca gctcttaacg taacattttt caatggaatt gttacttcaa
      601 ttttagtagc tgaatttgaa taagttttaa ctttaacatg cgccactgca tttggtacgt
      661 cattaaaata acgttccaac ttaccaattt tttcctcaat atagttgcga atagcatctg
      721 tgatagtgag gttatctcca tgaatttcaa atctaatcat agtaatctct ccttaaacct
      781 ctttattggt aactctttat tatattttaa catttttacg ctatcgtgca aacgcaaaca
      841 ctttgaattt tctgatattt ttagcatata atttacaccc tgcacgatga attgttaatc
      901 cagttgtata aatatcgtca acgagtaata tttccttgcc atttaaatct aattccgtat
      961 caataataaa tggattttca tctgccagac gctctttctt agttaaatga gactgttttg
     1021 gtctatttga catctttaaa atcttatcaa agcgaatccc tttagctttt agtactgctt
     1081 ctaccgggtt aaatgttcta gataaatcat gtgccggcga agaaggaatt ggcacaatat
     1141 agtcataaga tgtttgtggt atttcaatca aatgtgccaa taattcacat aaataatagt
     1201 ctttcaaaaa atttatactg atgtatcatc tctttcatta aaccgtcata ttgaaattga
     1261 caatataatt gttccattaa attaaagtgt gccgatagaa acttgcagtc taaacaatac
     1321 gcttcatctt gatttaagtg ttttaagcac cttgaacatc gccttgcttt aatatcaagt
     1381 ttaatattgt cccaattctc tttgcatctg tcacataatc tattaggttt cttgaacaaa
     1441 ttataaatgg ttatattttc atataactta gcaccacaac tcaaacaatt attcatcaat
     1501 ccaacctctt tttaatgcta atttgttcat cctttgaatc tcttttttag cttgaatcat
     1561 attcatactt actccttcat gaaaaaacaa tacttttcca gtaggacatt ctaattttcg
     1621 tccaacacgt ccagcaattt gtattaaagc ctcttgagta tattgatgtg catcgataac
     1681 aacaacatcc aaatttgcca ttgtaaatcc acgttctaat attgtcgtag taaaaatgac
     1741 atcgaaatgt ccattcctta attgttcaac tttttcaaag cgaaaaacat cctcgctatg
     1801 gacgtatgtt aatttagtaa ttttctgctt ataaaccgaa aatgttttaa tcattgtttc
     1861 tatattgtta aaaaacacca gtgtataacg ttgattatta atttgatctt gtaaaattcg
     1921 gtataacatt ttctgaatct tattattatt aagtttgaaa taacgatatt taggaactgg
     1981 aagtgatttt ttatgaaagc gagctggcaa tttaattata ttttcgtggg gaatctctga
     2041 cagaagttgt ttcggtggtg ttgctgtcat ataaattgtt gcatgttcaa ctttagaaga
     2101 tgacttcaat gcttgttgta gatttttatc cattgataaa ggaaaggcat cgacttcatc
     2161 aataaaaata gtatcaaagt gctgtttgaa tcggtaaagt tgatgcactg tgcatacaac
     2221 aaaatgccct tcaaattgtt gtcttgattg ctggtgtagt atgtctatat cttcattaag
     2281 aaatgcgtct ttaatacgtt tactaatttc tacaacaaca tctacacgtg gtgacacaat
     2341 agctatatta tctccctgta ttcttgcata ttgaatgcct tgaaacatca tttctgtctt
     2401 acctgcacct gttacggcat ataacaaaat cgtttgtctc tttctaatgg ctcgaacaat
     2461 atgttctgag gcataagact gctgttccga caattcaaat gagagatgat aatatgcttg
     2521 tgatgaactt tgcgaactct ctgttatttt atatcttgtt acattatcca ttcgacccag
     2581 tgatatacaa tttctgcaat atacaatttt cttggaatgt actgaggaat catactgata
     2641 aaaatgcttt ggatttgtac tttcacattg aacacaataa tagtttgaat tttgtttgat
     2701 gacacctgtt acttcttttt taattttttc attagtaagt gaagtatgag gatgaattaa
     2761 tttcccgtaa tgtttaatgc ttaaaacctc atttcaacgt aaaaaagagc ctaatattat
     2821 aatgattttc ttatatcttt agcattttgg ctttcaaatg atgaacattt aaagccatgt
//
LOCUS       DBNENW010000212         2863 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNENW010000212 DBNENW010000000
VERSION     DBNENW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.216"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            37..429
                     /locus_tag="AC1LW8_002580"
     CDS             37..429
                     /locus_tag="AC1LW8_002580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIP4774334.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADKPAVNKIMTEG"
     gene            431..>2863
                     /locus_tag="AC1LW8_002581"
     CDS             431..>2863
                     /locus_tag="AC1LW8_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HIP4774335.1"
                     /translation="MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFELLWQKSTPLTQQWIKSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSR"
BASE COUNT         1146 a          311 c          499 g          907 t
ORIGIN      
        1 acaattaaga tgattttaaa ttgaagggtg agtcttatga aaaaagtaat caatgtagta
       61 ggagctatta ttttttctga taacaaaatt ctttgtgcac agagaagtga agaaatgagt
      121 ctacctttaa tgtgggaatt tcctggcggt aagattgaaa agaatgaaac tgaaaaagaa
      181 gctttgatca gagaaattag agaagaaatg aaatgtgatc taattgttgg agacaaagtc
      241 ataactacag aacatgaata tgattttggc attgttaagt taacaacata caaatgtact
      301 ttaaacaaag agttaccaac tttaactgaa cataagagta ttgaatggtt gtcaataaac
      361 gaacttgata aattaaattg ggccccagcg gataaaccag ccgttaataa aattatgact
      421 gagggataat atgagtagat tactaaatga tttcaatcaa tctttaaaaa aaggttttat
      481 agacaaagat atttctcata aagggaatta tacaccgaag cttttagtaa ataataaaaa
      541 tgaaaaagtc ctttcaacaa ttatagatga acttcaaaaa tgtgaaacgt tctatttttc
      601 tgtagctttt ataacagaaa gcggattagc cagcttaaag gctcaactat tagatttaag
      661 caataagggt gttaaaggga aaatattaac atctaattac ttaggattta atagtcctaa
      721 aatgtatgga gaattactta aattaaaaaa tgtagaggta agattaactg atattgctgg
      781 attccatgcc aaaggatata tttttgaaca taaggattat agttctatgg taataggaag
      841 ctctaattta acatctaatg cgttaaaggt taattatgag cataatgttt tactgtctac
      901 tatgaaaaat ggcgatttag ttgatagtgt aaaaaatgaa tttgaattgt tatggcaaaa
      961 gagtacccca ctgactcagc aatggattaa atcatataaa gaatcatttg agtaccgctc
     1021 cttagaaaaa ttagcggagg ttgaacaaac tcaaatgctg ctagctgata aggtgaaaaa
     1081 atcagtggaa attgtaccca atttaatgca agcagaagca ttaaggtcat taaaagctat
     1141 tagggataaa gccaaagata aggcgttaat aatatctgca actggtacag gtaaaacgat
     1201 tttatgtgca ttagatgtta gagaagtaaa ccctaataaa tttttattta ttgttcataa
     1261 tgaggggatt ttaaatagag ctaaggaaga atttaaaaaa gtattaccta taaaaaatga
     1321 tagtgatttt ggattgttga caggaaaaca tagagatgtc gatgccaaat atttatttgc
     1381 aaccattcaa acattatcta gagatgataa ttttaagcaa tttgatgaaa atgaatttga
     1441 ttatattgtt tttgatgaag cacatcgttc tgctgcatct acttatcaac gagtatttaa
     1501 ctacttcaaa cctaagttca tgctgggaat gactgcaaca ccagaaagat cagatgaatt
     1561 aagtatattt gaactgtttg attataatat tgcgtatgaa ataaggttac aagcagcatt
     1621 agaaagtgat attttatgtc catttcatta ttttggtgtg actgattatg tacatcaagg
     1681 tattaaagaa gatgatgtaa ctaaactgag atatttaact tctgatgaaa gagttaatta
     1741 tattattcaa aagacagatt actatggata ttcaggtgaa attttacaag gattaatttt
     1801 tgtaagtagc aaaaaagaag cttatgattt agcggataaa ctaagttcaa aagggattaa
     1861 aagtgttgct ttaacaggag atgatagtgt taattatcgc caaattgtaa tagaaaaatt
     1921 aaaagaaggt aagattaact atattataac tgtagatctt tttaacgaag gtattgatat
     1981 tccagaagtc aatcaagttg taatgttgag accaacagag tctagtatta tattcattca
     2041 gcaacttggt agaggattaa gaaaaagttc aaataaagag tatgttacag taattgattt
     2101 tattggtaat tataagacaa attatttaat tccgattgcg ctttctgggg atcaatcgca
     2161 aaataaagat aattataaga agtttttaac gaataacgat tcgattaatg gagtatctac
     2221 aattaatttt gaagaagttg ctaaaaaaca gatttacaat tcattggatg cagtatcatt
     2281 aaatcaaaat aaattaatat taaaagctta tgaagaagtt gaaaatagat tgggacacat
     2341 gccgttacta atggatttca tacaacaaca ttctatagat ccaagcgtta tattttctaa
     2401 atttagtaat tattacgagt tcttagtgag atataaaaaa atagatacat tattgacgga
     2461 aaatgaatct aaaaatctgg ttttcttttc aagacaaata gcacctggat tgaaaagaat
     2521 cgatagttta gttttagaag aacttttaaa aaatgaatta acatatgatg aattaaaaaa
     2581 taaaatgttg aacgaagtta aggatataac agaagatgat atagatactt cattaagaat
     2641 tttagatttt tcattttaca acgcaggaat tgaaaaaatc tatggaagtc ccattataga
     2701 atgtaatgaa agaatgataa gactaagcga tgcatttact aatgctctaa gtaatcaaac
     2761 atttaaaata tttttagaag atcttattga attatcaaag tataacaatg aaaaatacca
     2821 aaaaggtaaa aatggactga ttttatataa taaatattca aga
//
LOCUS       DBNENW010000213         2856 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNENW010000213 DBNENW010000000
VERSION     DBNENW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.217"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            1..1677
                     /locus_tag="AC1LW8_002582"
                     /pseudo
     CDS             1..1677
                     /locus_tag="AC1LW8_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            1680..2000
                     /locus_tag="AC1LW8_002583"
     CDS             1680..2000
                     /locus_tag="AC1LW8_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIP4774336.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1997..2638
                     /locus_tag="AC1LW8_002584"
     CDS             1997..2638
                     /locus_tag="AC1LW8_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4774337.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(2726..>2856)
                     /locus_tag="AC1LW8_002585"
     CDS             complement(2726..>2856)
                     /locus_tag="AC1LW8_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774338.1"
                     /translation="MILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         1117 a          337 c          447 g          955 t
ORIGIN      
        1 tccttttaac gctaaatata acattcatta tttaagtgac gacgaattat taaaaaagat
       61 atcaaaggaa gttattttgt aaaagacaaa aattttgacg tgtctaaatt cataaatttt
      121 ttaaaagaat atggtgttac tgctgaatca tacaaaatag atcatatgat gattgccgtt
      181 ggtgtcatta aacaaatgaa tatagaagtt ccgttatccg cacttttaat cgtttatttt
      241 atttattata ttttcgaaaa gaacattaat ttcaaagcgt atgcgattaa gtatttaaat
      301 ggttttacat taagaaaaat aatttttgaa aatttttcaa aaaaatgcac gtattgggta
      361 acgttaatca taagtcaaat tcttttaact acaagtgtac tttggatatt aaattacacg
      421 ggtaatttag atttattcat attaagatta gttctgcttt catgtctttt cattttaacg
      481 attagtgtta ttaatttatg gactttctta atgttactaa atttaaatat tgctaatatg
      541 attaaaggta agcagcactt taaaacaatt cgttttatta atacagtttg taaaagtatt
      601 ctcttagtac taatagctaa tgtaatgata gaaaatacta gtgttattaa agatttgaat
      661 aaaataaaag aaactgagaa atattggaat gtattagatg attattacac gattgaattt
      721 gcaccttatc acgaaacaaa acaaagtttg attgataata tggtgcgatc agaacaatta
      781 gtaaaggcta gtgaagcaga aaataatgcg attttattca aaccaaaggg tgactccgtt
      841 gacaatgaca acttttcacc tgatgagggg aatgtaatat tagtaaataa tcagttttgg
      901 tcgatttatc acaagcagtt tcaacctgat attccaataa aaaatcaaaa aaataatgtc
      961 gaagtaatta ttccacaaaa gtttcatgca atgcgtaatg aaatcaatca agcatatcat
     1021 tcatggtttg aatttgtaca aaataaaaat aataaagaga gtaagttatc tatacagttt
     1081 atcaacaaaa atgattatcg aatttttaca tttgatgcac gagatagtcg ccatttgtca
     1141 tttatagagg cgccaatcat tgtgaatgtt caggcatcag atttatcgaa tgatttttat
     1201 tatgccatga tcagtcaagg cgggtattta ttcaaaaatt atgacgcgct agtaaaaaat
     1261 attgaaaagt atcatcttga tggggaaatc agtggaataa ccaattataa agatagcgtg
     1321 atggaaatgt atcatgaaaa caatttgaaa ttaacagtac tcaacttttc acaaatcatt
     1381 atcgcaatca ttttaataat tattatttta tttgatgtga aatattattt tgaacaacat
     1441 cgaaaattac tcgtaatcaa aaagctattt ggttattcaa cattaagagc caattaccaa
     1501 tacttattaa taaataatat agttgttgtt tttattggaa tattgacgaa tgtaatttta
     1561 cattctcagt atataatgat gatatttgca acgattattg ttgttcaaat attattgcag
     1621 atttgcagcc tatactatca tggccgacgt tttaatgaag ttatcaagga gttttaacta
     1681 tgaaatatat cattgcaata ttattaggat taatcgtatc cattactatt gcttttacaa
     1741 tcatacatca tcctatactt gatcgtttta atcctttctt aaaaacggag tatagttatg
     1801 ccaaagtgcc aaaaggtacg caacaatatg ttaatattac ggcttatagt gaaagagggg
     1861 aaaagcttga ttataaatta acatttaatg gattttcacc tagtagaacg tatgtcgaaa
     1921 taaagcataa agggcaatat gtcatatcga tcacatatgt tgaaaaagag gatacaccaa
     1981 aagaggtaag acaagaatga tagaattaaa agatttaacc atacaaaaag gtaatacaca
     2041 tattttgaag aaacttaatt tgaaatttca atgtggaaaa tcatatgcac ttattggtaa
     2101 aagtggatgc ggtaaatcta cattattaaa tactattgct ggacttgaaa aaacggggaa
     2161 acaatatgtc tattttaatg gtcaattaga acaatttaaa tctaattttt acagagataa
     2221 attaggatat ttatttcaaa attatggatt aatcgataat ttgacagtaa atgaaaattt
     2281 agatattgga ttagcatata aaaaaataag taagaaagaa aaagaacaat taaagatacg
     2341 ttatatagaa cagtttggtc tgtcaaacag tttaaaaaga aaagttcata cgctaagtgg
     2401 aggtgaacaa caacgtgtcg ctttaattag aatgatgtta aaagatccga ttgttatgtt
     2461 agctgatgaa ccaacgggtg cgttagatcc taaaacagga cagatgatta ttcaatcatt
     2521 atttgatttg gtcgatgaaa ataaagtgct gattttagca acacatgata tggctattgc
     2581 aaatcaatgt gacgaaataa tagatttaga acagtataga aaagtagcat ctatgtgata
     2641 tccaaaagat aaaattaaaa gccaatcaat gggaaatgtt gacttcaaca ctccgtttga
     2701 ttggctttta taatgtgtta ctaagttatg aaagtaacaa cggcaaactg aaaaaaagca
     2761 atataataat atttaatacg attagtaata aatgtaattg atttttcgtt ttaatatagc
     2821 aaaaaacaat gactaaagat aacaacaata tcatga
//
LOCUS       DBNENW010000214         2821 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNENW010000214 DBNENW010000000
VERSION     DBNENW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.218"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            117..716
                     /locus_tag="AC1LW8_002586"
     CDS             117..716
                     /locus_tag="AC1LW8_002586"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIP4774339.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1084..1685
                     /locus_tag="AC1LW8_002587"
                     /pseudo
     CDS             1084..1685
                     /locus_tag="AC1LW8_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1672..1809
                     /locus_tag="AC1LW8_002588"
     CDS             1672..1809
                     /locus_tag="AC1LW8_002588"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP4774340.1"
                     /translation="MNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRRRSTN
                     K"
     gene            complement(2000..2755)
                     /locus_tag="AC1LW8_002589"
     CDS             complement(2000..2755)
                     /locus_tag="AC1LW8_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP4774341.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
BASE COUNT         1027 a          442 c          477 g          875 t
ORIGIN      
        1 aatttcgaat atttaaattt tatataattg gatataacaa ataaataata attattgcaa
       61 aacacacccg aaattaatta ttataaaagt atattcataa aaggaggaat atacttatgg
      121 catttaaatt accaaattta ccatatgcat atgatgcatt ggaaccatat atagatcaaa
      181 gaacaatgga gtttcatcac gacaaacatc acaatacgta cgtgacgaaa ttaaacgcaa
      241 cagttgaagg aacagagtta gagcatcaat cattagcgga tatgattgct aacttagaca
      301 aggtaccgga agcgatgagg atgtcagtcc gtaataatgg cggtggtcat tttaaccatt
      361 cattattctg ggaaatacta tcacctaatt ctgaagaaaa aggtggcgta atagatgaca
      421 tcaaagcgca gtggggcact ttagatgaat ttaaaaatga atttgcaaat aaagcaacaa
      481 cattatttgg atcaggttgg acttggttag ttgttaatga tggcaaatta gaaattgtga
      541 caacgccaaa ccaagataat ccattaacag aaggcaaaac accaatctta ctatttgatg
      601 tttgggagca tgcctactat ctgaaatatc aaaataaacg tccagactat atgactgcat
      661 tttggaatat tgttaactgg aaaaaagttg atgaattata ccaagcagca aaataatata
      721 acttaatata attgaggtgg agcatctaca aggtgttcca cttttttgta cttaatatct
      781 ttacttattt gtttttgtgt aatgggatag cacgtaaaag tggcaaaata ataagataaa
      841 tttcaactcg aaatacctta ttattgttga ctataatgat gacctaaatg ttatacacat
      901 cagacaatac tattaattaa tcaacatatt gacattaaat aaattgacaa aataagtaat
      961 tattgtgaat ctaaagtgaa atttttataa aaaaatgtaa tgattcaaaa attttgttgc
     1021 atttcttttg taatcgtatg ataatgtaaa tgtaatcaaa ttgtaatata aggggacaag
     1081 acaatgaaaa aattagcaac agtaggttct ttaattgtaa caagcacttt agtattctca
     1141 agtatgcctt ttcaaaatgc gcatgccgac acaacttcaa tgaatgtgtc gaataaacaa
     1201 agccaaaatg tacaaaatca tcgtccttat ggcggagtag taccacaagg aatgacgcaa
     1261 gcacaatata ctgaattaga gaaagcttta tcccaattaa gcgctggcag taatatgcaa
     1321 gactataata tgaaattgta tgatgcgacg caaaatattg ctgataaata caatgtgata
     1381 attacaacta atgtaggggt atttaaacca catgctgtta gagatatgaa tggccatgcg
     1441 ttacctttaa caaaagatgg caatttttat caaacgaatg tagatgcaaa tggtattaat
     1501 catggtggta gtgaaatggt gcaaaataaa acaggtcata tgagtcaaca aggccatatg
     1561 aatcagaaca cacacatgaa ccaacagcca cacatgcaac aaggtcatat gcaatcatca
     1621 aaccatcaaa tgatgagtcc aaaagcaaat atgcattcat caaatcatca aatgaaccaa
     1681 agtaacaaaa aagttttacc agctgctggt gaaagtatga catcaagtat tcttactgca
     1741 agtattgccg cactactatt agtatctggg ttattcttag catttagacg acgttcaaca
     1801 aataaataaa cataatacga ttaataatag aaaaatcgtg tgattatctg agcgagccta
     1861 ggacataaat caatgtccta ggctcgctaa tgttatattg gcagtagttg actgaatgaa
     1921 attgcgcttg taacaagctt ttccatttct taccaactcc ctaaatagtc atcaaaaaat
     1981 ttcttatatt ttaataattt taataatccg attgtcttat acgtgtcagt gttaattcag
     2041 agatttcctg tggaatatac cacttattaa tcataattgg ataaggtgtt tgtgcgtaca
     2101 atgtttcaat aatcagccaa caatgtgtat caccatcaaa cacgtgacta tgatttttga
     2161 agtggggcgc tttggtaata gacattttta aatctgattg atatgcattg ctataaatcg
     2221 tttgctcaac gaatgtcttc atgtcgtctt cgttttgtgt attcacttta aatgtgtcaa
     2281 tgacatttaa cggtataaag gtaaagcaaa atgcatcagc ttgcttagaa tgattgtcct
     2341 ttttttgata atagcgttcc attgcaatga cggcagaagg atggtttgca aacaaatgat
     2401 ttgtatattc actttctaaa tcaacacgat aattaattga tgacatagat acgcgagcta
     2461 gcaatatttg atcaagtgga tgcttaaatt gatccatact tgaagcgtgt tgggcatttg
     2521 tttgtggaat aacaaagtgt cccttccctc ttgtactctc tacgatgcca tcttcggcta
     2581 acaattttat agcttggcgc aaagtcatac gactgacatc aaagcgcgca caaagttcct
     2641 tttcagtagg taatgcatgg ccactcggat attttcctat ttgaatttct ttatataacg
     2701 tattataaat cgttaaaaat tttggttgtg tttgcgtcac gtagacaacc tccataaagt
     2761 tacttaatca ctctcatcat acaataattt ttactcgaat tggaaaaatt ataaaaatta
     2821 a
//
LOCUS       DBNENW010000215         2819 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNENW010000215 DBNENW010000000
VERSION     DBNENW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.219"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            123..1058
                     /gene="lukE"
                     /locus_tag="AC1LW8_002590"
     CDS             123..1058
                     /gene="lukE"
                     /locus_tag="AC1LW8_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HIP4774342.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANVFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1060..2043
                     /gene="lukD"
                     /locus_tag="AC1LW8_002591"
     CDS             1060..2043
                     /gene="lukD"
                     /locus_tag="AC1LW8_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HIP4774343.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2285..2428
                     /locus_tag="AC1LW8_002592"
     CDS             2285..2428
                     /locus_tag="AC1LW8_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HIP4774344.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1077 a          376 c          471 g          895 t
ORIGIN      
        1 attgtgtcat ttttaatatt ttatagcatt ttatttttta atgaacagtt aattttataa
       61 ataagtgaaa taatctagaa taaatttgta ttaaaaattt atatagaaag aaagtgaagc
      121 ttatgtttaa gaaaaaaatg ttagctgcaa ctttgtcagt aggactgatt gcacctttag
      181 catctccgat tcaagaatct agagcaaata ctaatattga aaatattggt gatggtgctg
      241 aagtaatcaa acgtacggag gatgtaagta gtaagaaatg gggcgttact caaaatgtcc
      301 aattcgactt tgtaaaagat aaaaaatata acaaagacgc tttaattgtt aaaatgcaag
      361 gttttattaa ttccagaact tcattttcag atgtgaaggg tagtggatat gaattaacta
      421 aacgaatgat ttggccattc caatataata taggactgac gactaaagat ccaaatgtta
      481 gcttaatcaa ttaccttcct aaaaacaaaa tagaaactac tgatgttggt caaacattag
      541 gatataacat tggaggtaat ttccagtcag caccatctat aggtggcaat ggctcattta
      601 attattctaa aacaattagt tatacccaaa agagttatgt cagtgaagta gacaagcaaa
      661 actcaaaatc tgttaaatgg ggtgttaaag caaacgtatt tgttacgcct gatggaaaaa
      721 aatctgcgca tgatagatat ttattcgtac aaagtccaaa tggtccaaca ggttcagcaa
      781 gagaatattt tgctcctgat aatcaattgc cacctttagt tcaaagtggc tttaatccat
      841 cgtttatcac tacactatca catgaaaaag gttcaagtga tacgagtgaa tttgaaattt
      901 catatggtag aaacttagat attacatatg cgactttatt ccctagaact ggtatttacg
      961 cagaaagaaa gcataatgca tttgtaaata gaaactttgt agttagatat gaagttaatt
     1021 ggaaaacaca cgaaattaaa gtgaaaggac ataattaata tgaaaatgaa aaaattagtc
     1081 aaatcatcag ttgcttcatc aattgcactg cttttgctat cgaatacagt tgatgcagct
     1141 caacatatca cacctgtaag cgagaaaaaa gtagatgaca aaatcacttt atacaaaaca
     1201 acagcaacat ctgataatga taaattgaat atttctcaaa ttttaacgtt taatttcatt
     1261 aaggataaaa gttatgacaa agatacgtta gtacttaagg cagccggaaa cattaattca
     1321 ggttataaaa agcctaatcc aaaagattac aattactcac agttttattg gggcggtaag
     1381 tataatgttt cggttagttc agaatcaaat gatgctgtaa atgttgttga ctatgcacct
     1441 aaaaatcaaa atgaagaatt ccaagttcaa caaacattag gttattctta tggcggagat
     1501 attaatatat ctaatggctt atcaggtgga ttaaatggat caaaatcatt ttcagaaacg
     1561 ataaattata aacaagaaag ttacagaact acgattgata gaaaaacaaa tcataaatca
     1621 attggctggg gtgttgaggc gcacaaaatt atgaataatg gttggggacc atatggtaga
     1681 gatagttatg acccaacata tggtaatgaa ctgtttttag gtggtagaca aagtagttca
     1741 aatgctggtc aaaatttctt gccaacacat caaatgcctt tattggcgcg tggtaacttt
     1801 aacccagaat ttataagcgt actttctcat aaacaaaatg atacaaaaaa atctaaaatc
     1861 aaagtaactt accaaagaga aatggataga tatactaatc aatggaatcg actacactgg
     1921 gttggtaata actacaaaaa tcaaaataca gtaacgttta catctactta tgaagttgac
     1981 tggcaaaacc atactgttaa attaatcggt acggattcta aagaaactaa tcctggagta
     2041 taactgttag aaatagagga agttaaaccc gaaatttaag agttaaacaa aaaatattca
     2101 caacatatta aaaaaagaag ttacattatt tgtttaaact aacaatagta ccataaaatt
     2161 aatgacgatt tacaaattaa taacaaataa ttattattaa attcattgat ttattgtgtg
     2221 gtaatgatta atatgacaat tgaagtgggt cttaacttca acttttaaat agggggtttt
     2281 atctttggaa aaagttcttg atttagacgt gcaagttaaa ggaaataaca acactaatga
     2341 ttcagcgggt gacgaaagaa taactagcca tcttttttgt agctttggtt gtgaaaagac
     2401 gggtagtttt aacagcttct gttgttaagg atacagcaaa tagagcctga aagggctcta
     2461 tttgtctttt taaatatatt aagtgctaaa aaacggaaac agtttattaa tcgccaggta
     2521 tttttataat tagccttcta tattatcagt tgagtgtttc aataaattct gactttggtg
     2581 atggaaagtt atacttttag ttgtaatata gttttcatga cttcctgtta aataagttag
     2641 gattatgtaa ttagcctcta tctttacata taagtaatga caagctttgg ttattatatt
     2701 gaccctctta gaacatgtca tttaagaaaa taatcaagtc attgaaatta tatgtttata
     2761 tcctcatctt taaaataact tcaattatta tttgctaaat tttgtataaa tagaacaaa
//
LOCUS       DBNENW010000216         2809 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNENW010000216 DBNENW010000000
VERSION     DBNENW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.220"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            348..857
                     /locus_tag="AC1LW8_002593"
     CDS             348..857
                     /locus_tag="AC1LW8_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP4774345.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            869..1939
                     /locus_tag="AC1LW8_002594"
     CDS             869..1939
                     /locus_tag="AC1LW8_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIP4774346.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1956..2570
                     /locus_tag="AC1LW8_002595"
     CDS             1956..2570
                     /locus_tag="AC1LW8_002595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIP4774347.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
     gene            2687..2809
                     /locus_tag="AC1LW8_002596"
                     /pseudo
     CDS             2687..2809
                     /locus_tag="AC1LW8_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1022 a          359 c          505 g          923 t
ORIGIN      
        1 tttttaggga tgtatgtccc agcctcggcg tgtttatttt tattgacgtc taactctaaa
       61 agatataaat tagacgttta taaatgatgt aaataacgca atttctatca ttgctgttaa
      121 taattcatgg tttaatatac tatgagcata aactttttaa atagtattat tcactagttt
      181 taacaatcaa ttaattggta tatgatactt ttattggtta tttttatccc atagtgtgat
      241 aattactatt tttcattcat aataaaggtt taaagcatgt taatagtgtg taagattaac
      301 atgtactgaa aaacatgttt aacataacga tataaggagt gacgtacatg atccgtctag
      361 gtaaaatgtc agatttagat caaatcttaa atctagtaga agaagcaaaa gaattaatga
      421 aagaacacga caacgagcaa tgggacgatc agtaccctct tttagaacat tttgaagaag
      481 atattgctaa agattatttg tacgtattag aggaaaatga caaaatttat ggctttattg
      541 ttgtcgacca agaccaagca gaatggtatg atgacattga ctggccagta aatagagaag
      601 gcgcctttgt tattcatcga ttaactggtt cgaaagaata taaaggagct gctacagaat
      661 tattcaatta tgttattgat gtagttaaag cacgtggtgc agaagttatt ttaacggaca
      721 cctttgcgtt aaacaaacct gcacaaggtt tatttgcgaa atttggattt cataaggtcg
      781 gtgaacaatt aatggaatat ccgccatatg ataaaggtga accattttat gcatattata
      841 aaaatttaaa agaatagagg taatattaat gacgaaaatc gcatttaccg gagggggaac
      901 agttggacac gtatcagtaa atttaagttt aattccaact gcattatcac aaggttatga
      961 agcgctttat attggttcta aaaatggtat tgaaagagaa atgattgaat cacaactacc
     1021 agaaattaag tattatccta tttcgagtgg taaattaaga agatatattt ctttagaaaa
     1081 tgccaaagac gtatttaaag tattgaaagg tattcttgat gctcgtaaag ttttgaaaaa
     1141 agaaaaacct gatctattat tttcaaaagg tggatttgta tctgtgcctg ttgttattgc
     1201 agccaaatca ttaaatatac caactattat tcatgaatct gacttaacac caggattagc
     1261 gaataagata gcacttaaat ttgccaagaa aatatataca acatttgaag aaacgctaaa
     1321 ctacttacct aaagagaaag ctgattttat tggagcaaca attcgagaag atttaaaaaa
     1381 tggtaatgca cataatggtt atcaattaac aggctttaat gaaaataaaa aagttttact
     1441 cgttatgggt ggaagcttag gaagtaaaaa attaaatagc attattcgcg aaaacttaga
     1501 tgcattatta caacaatatc aagtgataca tttaactggt aaaggattaa aagatgctca
     1561 agttaaaaaa tcaggatata tacaatatga atttgttaaa gaggatttaa cagatttatt
     1621 agcaattacg gacacagtaa taagtagagc aggatcaaat gcgatttatg agttcttaac
     1681 attacgtata ccaatgttat tagtaccatt aggtttagat caatcccgag gcgaccaaat
     1741 tgacaatgca aatcattttg ctgataaagg ttatgctaaa gcgattgatg aagaacaatt
     1801 aacagcacaa attttattac aagaactaaa tgaaatggaa caggaaagaa ctcgaattat
     1861 caataatatg aaatcgtatg aacaaagtta tacgaaagaa gctttatttg ataagatgat
     1921 taaagatgca ttgaattaat ggggggtaat gctttatgag tcaatggaaa cgtatctctt
     1981 tgctcatcgt ttttacattg gtttttggaa ttatcgcgtt tttccacgaa tcaagactag
     2041 ggaaatggat tgataatgaa gtttatgaat ttatatattc atcagagagc ttcataacga
     2101 catctatcat gcttggggct actaaagtag gtgaagtctg ggcaatgtta tgtatttcat
     2161 tacttcttgt ggcatatctc atgttaaagc gccacaaaat tgaagcatta ttttttgcat
     2221 taacaatggc attatctgga attttgaatc cagcattaaa aaatatattc gatagagaaa
     2281 gacctacatt gctgcgttta attgatataa caggatttag ttttcctagc ggtcatgcta
     2341 tgggatcaac tgcatatttt ggaagtggta tctatctatt aaatcgatta aatcaaggta
     2401 attcaaaagg tattcttata gggttatgtg cagctatgat tttattgatt tccatatcac
     2461 gtgtatatct aggtgtacat tatccaacag atattattgc cggcattatt ggtggcgtat
     2521 tttgcattat tttatcaacg ttattactta gaaataaatt aataaattaa atagtaaaaa
     2581 aacaaaagca gtaaacctaa agtgtcgtaa gggtttactg cttttataaa acgttgttat
     2641 aacgtatatt gtcttttacg ggcatataaa aggggaatat ttgaaaatga ccaatccaac
     2701 aagaacgaaa cgttgtgggg ggatgttcta tgtggtattg ataatcattt tcaactacta
     2761 ttatacatta gtgagaatca ttgtcaatta gaaattaaaa ctttttttg
//
LOCUS       DBNENW010000217         2789 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNENW010000217 DBNENW010000000
VERSION     DBNENW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.221"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            179..382
                     /locus_tag="AC1LW8_002597"
     CDS             179..382
                     /locus_tag="AC1LW8_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HIP4774348.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(379..615)
                     /locus_tag="AC1LW8_002598"
     CDS             complement(379..615)
                     /locus_tag="AC1LW8_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774349.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            839..1420
                     /locus_tag="AC1LW8_002599"
     CDS             839..1420
                     /locus_tag="AC1LW8_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP4774350.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1493..2110)
                     /locus_tag="AC1LW8_002600"
     CDS             complement(1493..2110)
                     /locus_tag="AC1LW8_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HIP4774351.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     AAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGEMVGSIDKTGKLLTIINKLSSIIIIIVALMILQKLIQLLF"
     gene            complement(2265..2759)
                     /locus_tag="AC1LW8_002601"
     CDS             complement(2265..2759)
                     /locus_tag="AC1LW8_002601"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774352.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT         1013 a          419 c          416 g          941 t
ORIGIN      
        1 tatgaaaaga atatttacat actaaaaact cttaaagttg tataattcgt taatattttt
       61 agaatcagat aacaaaaaat gtgcaaaatc acttttgcac atttacaaat actagttttc
      121 tgataagatt aataataaca ctttaaatag cgcttaataa atgaaagggg caagtcttat
      181 gacgttttac aatttcatca tgggttttca aaatgataac acaccatttg gtatattggc
      241 cgaacacgtt agtgaagata aagcattccc tcgattagaa gaaagacacc aagtaattag
      301 agcatatgtg atgtctaatt acacagatca tcaattaatt gaaactacaa atagagctat
      361 tagcttatat atggcaaatt aatttgagta gtaccaatta tgatgtatta gtgcatccca
      421 aatatctttt gttttaaagt ttatttcatc atttcttatc gaaaatggtg taataatgtc
      481 tttatctaac caagtgttga taagttcatt tggtacacca tctaacaaca tttcactttt
      541 actaattata aaacattccc agtcaagtga aacattttgt ggattcacat aattacattg
      601 attatgatta tccataaaca ctcactcctt taaaattctg tactcttcat tgcgttttac
      661 cccgtcacat tatcttttaa actaaaatca tcattactta tgaaaaaatg tacatcaaaa
      721 gcaaaggttt tcgctaccga aaaagtttaa ataatggttt aatatatttg gtactcattt
      781 taataaaaag agaatacatt ttgagctatc aatacttttt attgaagagg tgttattgtt
      841 ggctaaaacg ttatatttaa tgcgccacgg acaaactttg tttaatttta agggactaat
      901 tcagggattt ggagattcgc cgctaacaga acttggaatt gctcaagctc aaaaggcacg
      961 tagttattat gaaactaagg ggataaactt cgatttatat gcatcatcaa cgcaagaacg
     1021 cgcaagtgac acacttgaaa atgttgcacc taaccaatcg tatcaacgtt ttaagggact
     1081 gaaggaatgg cattttggat tatttgaagg tgagtcagtc tatctatttg ataatctata
     1141 caagcctgaa gacttattcg gagatcgaat tgttcctttc aaaggagagg caaggcaaca
     1201 agttgaagat cgcattgtga aaactttaca tgacattatg tctcaaacaa agaataatgc
     1261 attagtcgtg agtcatggaa caataatggg agtattttta agatattgcc ttaaactaga
     1321 tgaagcatta aagcataata tcggtaattg taatatcctg aaatttgaat atgacaatgg
     1381 aacatttaaa tttgttgagt taattgatcc aaatttataa taaataaagc gttatacata
     1441 gacacaagca tgtcttttgt ataacgcttt taaaatacaa ctatataacg ctttagaata
     1501 ataattgaat aagtttttgt aatatcatca gagcaacaat aataattatt atgcttgata
     1561 atttattgat tattgttaat aattttccgg ttttatcaat tgatccaacc atttctccta
     1621 aaattgcgag taaaaagaac cataaccatg aaacgctaat acaagcgatt gtaaacgcaa
     1681 ttttattgct gccactatat aatgcagcac tactaccaat tacaccaatt gtatctaaaa
     1741 tagcatgtgg attgagtaat gaaactgata aagcaaaact tacttgtttc attggagaca
     1801 taatttgagc ttctccatct gttgagggtt tatcatgcca aatggtccaa gccatataca
     1861 tcaagaaaat taaaccaact atataaataa ttgcttgaag tacaggtaaa gacataataa
     1921 tgatagatac tcctaccact gcaataataa taagtaagct gtctgacaac ccggctgcaa
     1981 ttattgcagg caatacatat ctatattttg gttgattagc tccttggtta aaaataaata
     2041 cattttgtgc acctaaaggt aaaattagac caatcgctaa gataaatcca tgaataattg
     2101 cggttaccat taagttttga cacctctaaa acatagaata ttctgaaaaa acttattgtt
     2161 atcatatcaa ataaaaaaca actactcaat agtagagtag ttgtatgaaa aatagaacct
     2221 atatgatttg aatttatgag aaatgtagaa tatataaaac tgtattactt attaatcaaa
     2281 tcataaaaaa gccaatgttc atcgaaatag gtatttccga ttttaattgc atttttctca
     2341 aaaccaagaa tgtcgaatcc tagtgcacta tagaaaactt tagcacctat gttgtttgac
     2401 acaattgatg taagtaaaga ttcgtaattg ttctgccgtg cataattaat aatgaaatta
     2461 atgagctcgc tattaatcga tttatcgtta ttagtaacaa agttgtattt aattaatgat
     2521 ttatgttctt ttccaacgta tcgaatttgt tctaaggcag ctgttgcgat aagtgtttcg
     2581 ttttcataac agccaaatac aatacatttc ggtgaacctt gatttaaaat gtcatgtatt
     2641 aaggtgtcag tcattgtttg actataatgt gaatcttgag tgaattcttc attgactgtc
     2701 gaaacaagtc tcttatactc attaaaatca ttattcgtta atgttttaat ttctatcatt
     2761 actgacctcc ttaatcatta ttaagaaag
//
LOCUS       DBNENW010000218         2783 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.222, whole genome shotgun sequence.
ACCESSION   DBNENW010000218 DBNENW010000000
VERSION     DBNENW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.222"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(97..1356)
                     /gene="femB"
                     /locus_tag="AC1LW8_002602"
     CDS             complement(97..1356)
                     /gene="femB"
                     /locus_tag="AC1LW8_002602"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HIP4774353.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            complement(1375..2637)
                     /gene="femA"
                     /locus_tag="AC1LW8_002603"
     CDS             complement(1375..2637)
                     /gene="femA"
                     /locus_tag="AC1LW8_002603"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HIP4774354.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
BASE COUNT          892 a          503 c          364 g         1024 t
ORIGIN      
        1 agtttttctt agtgtgaaat aattaaaatt tcgctaaaaa aagcataaaa aagagcctat
       61 agtatcaata actataagcc ctaacatcat ttacatctat ttctttaatt ttttacgtag
      121 tttatccaat gttgtgaaca accaatattt cactttatga attggtttat agaaatcccc
      181 tattaattct tcgatttgta cattaaatcc acgtttaaag cggtatacgc cataatcttc
      241 actgttttcc gtaaaatcac ctgataaacc atagaaatta taacgatcat aaccattatc
      301 gaagcaatag ttaatcataa accaatgcat catgtatggt cccataaatt gattatattt
      361 ttctgatgaa ccaccagaga aataattcac ttcatatgca tttgcaaaat aaacaccaga
      421 agcaaggttt agaattgggc cgtccgtttt gctcaattca cttgcattca ataattcatg
      481 ctgatcatga tcaatttgct tatctaattc tgcaattttc ttcatttgtt tatctgattt
      541 gttttctttc gccatcattt gatcacgacg attttctttg tcattcaatt cctgttgcag
      601 ctttaacaca tattcatcaa ggtcaatata tgctaatggt actaatactt tatctccata
      661 tgtgtcaata aagttataaa aataatcatc tgtttttgat acaaatccag cacgctcttc
      721 agtttcacga tataaatcta ggaaaagatt gaactcatca cgttcaagga atctgacttt
      781 aacaccatag tttatcgctt tattaatatt acgtttacgt tgactatcaa atgtcttttt
      841 caatgtttcg ggtgttttac cttcaaggtt taatacgccc atccatcgta cttggctcga
      901 tgtatcatac tcagttgtaa agccatgatg ctcgtaacca tgtgatttaa acaagtttac
      961 tagggcatca tttttctcgc gaccttcaaa tggcacgata tctttatcat atagatgata
     1021 taaccaatac ggatctaatt taacatataa acattgatgt tgctgtaaat atttatctaa
     1081 ctcttttaaa taataatcaa ctaatcctaa atctgaaaaa tccattactg gaccacgatt
     1141 cgaatagtaa acataacttc ccatagtagg aattttagag aaaaggcttg ctgcaattac
     1201 tttgttattg tcgtctttaa tacctaataa aactacttca aagccatcat tctcacgggt
     1261 aactatattt tcttttactt ggaaataatg actttccaat gatggatttt gtacaaagtt
     1321 gtcaaattcg gtaacagtta actctgtaaa tttcatgttt tgataattcc cttcctaaaa
     1381 aattctgtct ttaacttttt taagtgcggt atatgctgcg taaacaggtt tattaattgg
     1441 tttaataaag tcaccaacat attcaataat ttcagcattg taaccttttt tgaatttaac
     1501 tacaccagca tcttcagcat cttctgtaaa tttaccacta acaccataga aattataacg
     1561 gtcaatgcca tgatttaatg cataattaat catttcccat tgcactgcat aacttccggc
     1621 aaaatgacgg aaagcatttg atgtaccacc agcataataa acaacttcaa atgggttgat
     1681 aaagaagaaa ccagcagaga taggtaattc attaccatgt tcttcttgta gacgtttacc
     1741 ttcttcaatc ttttgctcat tagcatcaag ttgttgttgt aagttatctc gcttgttgtg
     1801 tgctttttta ttttcaggac gtttttcaat atcctttaac gctttattta aatctttatt
     1861 taaaatatca cgctcttcgt ttagttcttt aatatattca tcaaagttga tatacgctaa
     1921 aggtactaac acacggtctt tgtaatattt taagcgattg tagtaaaatt tgtcatcacg
     1981 atcagcaaaa gcttttgatt ctgacgtatc ttccataaat gatctaaaaa ttggtagttc
     2041 ttcttcagat aaaaatctta ctttaacacc attcttttta acttttttcg tgtttctttt
     2101 tctaagtcca tccatatttt taatgatgtc atctgctgtt ttatctttta aatctaacac
     2161 tgagtgataa cgaatttgta gcacaggatc aaatccttta tggaatccag tatgttcaaa
     2221 tcctaagtta ctcattttat caaagaacca atcattacca gcattacctg taatctcgcc
     2281 atcatgattc aagtattgat atggtaaata tggatcgata tgtaggtata gacaacgatg
     2341 ttttttaaca tattttgata attcattaaa gaaaaagtgt acgagttctt gattttcata
     2401 atcaatcact ggaccgcgat ttgaataaaa atacttgaac actttcataa caggtacagc
     2461 agtaagtaag caagctgcaa tgacctcgtt attattgttt tttattccca ctaaatgtgt
     2521 ttcataacct tcagcaagct ttaactcata gtggccaaca gtttgcgtga aatgactgta
     2581 tggcatgcta tctgtaaatg caccaaactc tttagctgtt aaatttgtaa acttcattat
     2641 cattactcct atttgtctct cgttaattaa tttcatttcc gtatttgcag tttttctatt
     2701 tcccctctgc aaatgtcaaa aataataaat ctaatctaaa taagtataca atagttaatg
     2761 ttaaaactaa aacataaacg ctt
//
LOCUS       DBNENW010000219         2761 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNENW010000219 DBNENW010000000
VERSION     DBNENW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.223"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            23..283
                     /gene="tsaA"
                     /locus_tag="AC1LW8_002604"
     CDS             23..283
                     /gene="tsaA"
                     /locus_tag="AC1LW8_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin TsaA"
                     /protein_id="HIP4774355.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            312..500
                     /gene="tsaT"
                     /locus_tag="AC1LW8_002605"
     CDS             312..500
                     /gene="tsaT"
                     /locus_tag="AC1LW8_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin TsaT"
                     /protein_id="HIP4774356.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            688..1257
                     /gene="lnsA"
                     /locus_tag="AC1LW8_002606"
     CDS             688..1257
                     /gene="lnsA"
                     /locus_tag="AC1LW8_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein N-acylation protein LnsA"
                     /protein_id="HIP4774357.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1345..1629
                     /locus_tag="AC1LW8_002607"
     CDS             1345..1629
                     /locus_tag="AC1LW8_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774358.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1691..1909
                     /locus_tag="AC1LW8_002608"
     CDS             1691..1909
                     /locus_tag="AC1LW8_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774359.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(2405..2605)
                     /locus_tag="AC1LW8_002609"
     CDS             complement(2405..2605)
                     /locus_tag="AC1LW8_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIP4774360.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          967 a          415 c          378 g         1001 t
ORIGIN      
        1 tttatactag taggtgtcag tcatgaataa cattttgtta aatgctatca atatagttat
       61 tactaccact tttgttatct ttaatatttt aatcacatat aataaagatt tagatgattt
      121 atgttggctc ctgcctggta ttatcatttg tggtgtgata ctcatcgtat cctttaccat
      181 tgcaatgata actaaaaact ggttaagtga aatattattt tttataaata tcgtactcgt
      241 tctctattac atttatccta ttttttatag ttttataggt taataattac ggtatataag
      301 gagggtttca catgtcttta cattttgcaa ttctgttttg gctagcatta attttcttag
      361 ttgccgctac gtttatactc gtattaatga aaaaaactgg caaagaatct aaaaaagagt
      421 cctatttaag tttcactgtc attctctata tttttggatt cgctatatta atatacacat
      481 ttatatttgg tgtgctataa atgaaattca ttttatatcc agtctgaaac gtctcaatga
      541 tgtttcagac tgttttttat tcttaataga catttcacac tctcatactt taatatttaa
      601 gatatgtttt agtcttacaa gcttattaaa gattacaatt ctaatttaag actatttaca
      661 tatatttctt caacaaggag cttttaagtg aactatatta aacgaacgat aattttactt
      721 atattattcg ttgtcgtatc cccaataaac tcaccaaaaa caattgcaga taataagtat
      781 tcagaaattc aagatgacaa attccaatta caacctggtg atattattgt tacaaaaggc
      841 cctgtcatgt ggggattttt tggtcattgt agtatcgcga ttgatgataa aacgatttta
      901 caaattgagg ggcctggcga caaaccaact acacaatcct tcgaatcttt taaatataat
      961 tatgcaagtg gcaaaaatga ttggatgaaa gtttatcgtt gcagctatcc tggtgcaggt
     1021 aaaaaagcgg ctgaatgggt taagaaaaac tatgagaata caaatcatcg ttatctagtt
     1081 acattaaatt tgaacagcaa aaaatttacg tattgtacta aaattatcta ccaagcttat
     1141 aaatttggtg taagtgaaaa atcagttaaa agttatggat tacatattat ttcaccctat
     1201 gcaattaaag ataattttat agatccatac aaactaaggc ttgttaaagc ttattaacac
     1261 agtttcatca aatagttaaa ttagtttgtg ttttgcaata aattgggtat agattacaaa
     1321 tgatatttca ggaggcccaa tattttgaat gaactaacta aagaacaaaa gtatacaata
     1381 gctaaatttt acaaattata tattgaacgt tcgaataatg gagaaacaga aacagtcgct
     1441 aacttttttg gcgatgctaa agatgcacgt gaaaactatt tttgtgatcg tgattatcaa
     1501 gacttcttaa ctaactgcca aattttaatt caaaacaaat acttaactgg tgaagttttg
     1561 gacgataaca tttacaatat ttctatttta aacaagacat ttattgaatt cgaacaaaat
     1621 tttggttgaa catgaatgtc tcaactataa actttctaac cctattttaa agcaggagtg
     1681 aaaaacatac atgagtgaat ggcatattaa taatcaatct acaaaacctt ttttaataca
     1741 acaagcggaa aagcaattgt ctattgttaa accgttgcct aatggtagct tccaaatact
     1801 cacagaaata aatttggaga atggcaatat tagtaacatc gatcattctt tacttgtttc
     1861 agtagacccc aaagcattag aaataagtat atttgacgct gtaaattaaa cgcaatattt
     1921 ttatttaatt attttaatgt ttcgcctttt aaatgaaaac aagattactg aattattatg
     1981 agattttaaa aacaataaat attctcaata attaatcagt cttaataaaa aattaacaaa
     2041 aagttgatta gttaaattat taattaagta gtaagatatt atgcgatgtg gtatcctatt
     2101 attgttaaac attttgagtg tttaacatta cttcttgtta tcgccatata cagactttaa
     2161 ttctcgtcat tattaaagtt ctgtattggc gcttttttat attcaaaatc aaaagtcgga
     2221 cagatgaaaa gtaaaaaact tttccatcag ttcgatctat tttagaacca ttatatattg
     2281 ttaaatctcc acctaaattt attggacaaa aaaataacgg ctagtgcttt taccacacca
     2341 actgttatcg atttaaagca ttaaaaaaca gccctataat aaggactgtt tgaataagtt
     2401 aaaattacat tttaactacg tttgcagctt gctcgccacg gtcgccttca acgatgtcga
     2461 attcaacttt ttggccttct tctaatgatt tgtatccatc ttcagcgatt gctgagaagt
     2521 gtacgaatac gtcgctacca tcttctcttt cgatgaaacc aaaacctttt tctgcattaa
     2581 accatttaac tgtaccgtta ttcatattga atacctccgt cgtgcttttg cactaaatat
     2641 tttgtaacaa attcataaat gaaaaggaga atattctata taaataactc acatttttct
     2701 tcacgctctt tattacttaa ctttcattat acacttttaa atatagaagt aaagcattat
     2761 t
//
LOCUS       DBNENW010000220         2677 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNENW010000220 DBNENW010000000
VERSION     DBNENW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.224"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            219..1628
                     /locus_tag="AC1LW8_002610"
     CDS             219..1628
                     /locus_tag="AC1LW8_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="HIP4774361.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDVL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1632..2258
                     /locus_tag="AC1LW8_002611"
     CDS             1632..2258
                     /locus_tag="AC1LW8_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774362.1"
                     /translation="MLFNVKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENKVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            2394..>2677
                     /locus_tag="AC1LW8_002612"
     CDS             2394..>2677
                     /locus_tag="AC1LW8_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774363.1"
                     /translation="MLYESNIINKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFINWLI
                     PKKYVQISREEFDNLNIVKPVKNKALWPVIGSILLFGTMFRDKIYIPDSH"
BASE COUNT         1009 a          319 c          484 g          865 t
ORIGIN      
        1 tttttattga ttaattgcga atatacaaag acaccattat atccatatgc taaaataaga
       61 aaaaaggcga catgttatct caatttacct gcatctagac tcctttacat aagaaataat
      121 catcgccgag ttttaaaatt ttacaacttt gtagatgtaa aaagcatttg attttaaata
      181 gtttgaaaat catatatttt tgaaaaagga gcatgccgat gagtattgac atgtatttag
      241 acagatctcg aaaccaagct tcaagtgtgg ggaatttgag tcaaacaatg aattcaaatt
      301 atgatgcgtt ggaaaaagca attactcaat ttatcaatga tgatgtgctt aaagggaaag
      361 cgtatacgtc agctaagcaa ttttttagta cggtgttaat tccattatca acaagtatga
      421 aaacattgag tgatttaacg aagcaagctt gcgataattt tgtgtcacgt tatacgagtg
      481 aggttgatag catatcttta aaagaatcag agcttgaaga agatatcaga tcattaagtc
      541 aacaaattac gcgatatgaa aatttgaata acaatttgaa aaagcatgct tccgataatc
      601 agcaagccat ttcatcgaac caacaaataa tacaaacatt aggtcaacaa aaacatgaat
      661 tagaagagaa gctacgcaaa ttgcgtgagt ttaatcaaaa atcaccagaa atatttaaag
      721 aagttgaaga atttcaaaaa attgtccaac aaggacttac ccaagcgcaa aatttttgga
      781 acttttcaac aaatcaattt aatattcctt caggtaaaga acttgattgg gccaaagcaa
      841 gtcatgaaaa atatttgaaa gttgctatgg ggaaaattga acataaagca gagaaagaaa
      901 ctttaaataa agcagacttt gctgttataa aggcatatgc caaagaacat ccagaagacg
      961 atatcccgaa aagtatattg aaatatataa atgacaataa agacagtatt aaaagagata
     1021 taggattaga tattacgtca acacttttag agcaaggcgg tataaatgca agtaaattcg
     1081 gtgtatttat caatacagca ggtggagtga aaggcccagc aggtccaaat tcatttgtgg
     1141 aagtcaaacg tacatcaggt aatgtgttta tagaaaatgg tagtaaattt gcaaaaggcg
     1201 gaaaatacct aggtaaaggt gttgctggtg taggatttgg tataggtatg tatgatgacc
     1261 ttgcaaatga tgataaaaca tttggagagg cgttgtcgca taatggtatg acgcttgcag
     1321 ctggatctgc agggacagca gttggagctg gattagcgac ttttgtttta ggaagtaatc
     1381 cagtaggatg ggtgatttta gcaggtttgg ctatgagtac agtatttgca ttaggaacag
     1441 atttaattta tcaaaataat atttttggat taaaagataa agtagactgg gtagggcata
     1501 aaatcgataa tagtatagat gttgttaaaa aaactacaga aaaatccatg gatagtgttg
     1561 ggaatgctgt tagtgaagct aaaaatatta ttagcaatca tataaatcca atgaaatggg
     1621 cgtggtgagt tttgcttttt aatgttaagg ttattaataa aaatccgaga tataaagtcg
     1681 ttcaatataa tgatgagtat ttgttaattg atttagtaag cacttggctt gtatactttt
     1741 ttccttttat taattggttc attcccaaaa ggtatgcaaa acttagtgag aaggaacttg
     1801 aaaatttaaa tgttgataaa caaaataaaa ataatatttt ctggccagtt actggtagtt
     1861 cgtttttatt tgtagttatt ttacggaaat atgtacatac gtttgaagtt caattagata
     1921 ataagatttt aatatcttta tgttttatag gatttatagg tattgcagca ttttacattt
     1981 acttaaataa aaagcttaaa ctaaagatat atgatgataa tctagataat gaaaataagg
     2041 ttatattagt gcctactttt aaagatggaa gttttatagt tttcacatat ctattattag
     2101 gaggttgttc tatattattt ttaatatggc taatgactat aaaacctcaa aatctactgg
     2161 tatttattat gtggattatt attacaattt ttttctttct aataagcatg ggctcaatta
     2221 gtaataaaaa ggtttatgcc aaacttaaaa agcaatagta attactaaac ttagattgta
     2281 gttcgtaagt taagtaataa acaggaaaat agtatgttac taaaatacaa aacacttata
     2341 aagtatctga aagtgtatta gatataaaac caacgagacg gaagtggtaa attttgcttt
     2401 acgaatctaa tattattaac aaaaatccca gatatagaat tattaaatat aaaaacgact
     2461 atctgatgat tgatttagta agcacatggt tagttttgtt ctttccgttt attaattggt
     2521 taatcccaaa aaaatatgtc caaatcagca gagaagagtt tgataattta aatattgtta
     2581 agccagttaa aaataaagct ttatggccag ttataggtag catactttta tttggcacta
     2641 tgtttagaga taaaatatat atacctgatt ctcattt
//
LOCUS       DBNENW010000221         2614 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNENW010000221 DBNENW010000000
VERSION     DBNENW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.225"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(92..1135)
                     /gene="recA"
                     /locus_tag="AC1LW8_002613"
     CDS             complement(92..1135)
                     /gene="recA"
                     /locus_tag="AC1LW8_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIP4774364.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(1300..2451)
                     /locus_tag="AC1LW8_002614"
     CDS             complement(1300..2451)
                     /locus_tag="AC1LW8_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIP4774365.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
BASE COUNT          801 a          503 c          347 g          963 t
ORIGIN      
        1 gatattattc tagctttatg agatgaatat cactttcaat caatcctata aggatatttg
       61 ctaagtttaa ctatagatat aaatttgtgt actattcttc gtcaaataat gactttggtg
      121 catcttctgt ttcttcaaca tcaccatcag atatacctaa tttttctctc aatttacgat
      181 caatttcttc tttaatttgt ggattttctt tcaagtacat tttaacattt tccttacctt
      241 gacccattcg ttcgccattg taagaatacc atgctcctga tttatcaacg atgtcgtttt
      301 caacacctaa atcaataagt tcaccctctt tagaaatacc ttgtccatac ataatgtcaa
      361 cttcagctac tctaaatggt ggtgccactt tatttttaac gactttaatt ttagttctat
      421 tacctacaat ttcttgtcct tgtttaagct gttctgcacg acgtacttct agtcttactg
      481 aactatagaa ttttaatgca cgtccacctg gtgtagtctc tggattaccg aacataacac
      541 caactttttc acgaatttgg ttgatgaaaa tagcagttgt atttgattta gaaatagcac
      601 ctgaaagttt acgtaacgct tgtgacatta aacgagcttg taaaccaacg tgagtgtctc
      661 ccatttctcc ttcaatttca gctttaggtg ttaaagcagc aactgagtct acaactacaa
      721 tatcaactgc accacttcta acaaatgctt cggcgatttc aagaccttgt tcaccatgat
      781 ccggttgcga taaatataaa ttatcgatat ctacgcctaa tgcttgagca tattctggat
      841 ctaaagcatg ttcagcatcg ataaatgctg ccaccccgcc attactttgt acttcagcaa
      901 tagcgtgaag cgctactgtt gtcttaccag aactttcagg accataaatt tcaataattc
      961 gtcctttagg atagccacct acacctagcg cattatctaa tgtaactgaa ccagttgatg
     1021 tagttgaaac tcggcgacct atattgtcac ccaacttcat tacggcacct ttaccgaaag
     1081 atttctccat atttttaatt actgtatcta aagctttttg acgatcgtta tccaaagcga
     1141 gacctcctaa ttgaaattgc tatcttttaa ttatcttacc aatactatac aaggattcaa
     1201 aataaaatac aagtgttttg cgaatatttg ttcgtataaa atcttgaaat caaaacgcaa
     1261 aaacgaacaa acgtgcttat atttttatcg ataatacaat taatttgttg ttctaagcca
     1321 atttaataat cttatgagcg cataattttg acttctgttc tttaataaat ttctagattt
     1381 agacatttta aattgctcag ttttaaattc aaagttatta tcatagccta tatggactac
     1441 accatcatac tctaatagaa taattgctga tgacacatta aacaatttat taacaaattg
     1501 cactgcatct tttaattgct gctcaatagg tttattaata tcaacaaagt tttcattgtg
     1561 attaatcata ccctttagaa catcgtttaa atccacttct ttcaatcgat gatataaagc
     1621 accattagta ataccatcat atattacaaa aggttcatga attttcttta ttacagcttg
     1681 ctcaattaat gtgtcatctg aaccataata atattctcca atacgatcaa gaatttcttg
     1741 tttaacaggt tgaatcaatg attgtgcttg ttcttttgag tcggcatttg cagtcaatct
     1801 aatatatact tcatgacttc ccgccaaagg cgcaatcgta ggattagtct gtttatcgat
     1861 aagatctatt aatattgttt ctactttaga ttcacctatt cccgcaaatc ttaatagttc
     1921 agaatgtata attcgattat ggtttataaa atgtgacaac aattcatttt tcaccattgg
     1981 ttgcatttct ttcggtggac ctggtaataa aataatttgt ttgttttcaa aattcaccat
     2041 cattcctgga gccatgccat gatgatttgt taatacagtt gaaccttcaa ttactaaagc
     2101 ctgttgttta ttattaggtg tcatttcttg tccttgttcc tcaaaatagc tttcaatata
     2161 ttttaaagaa ggctcatcaa taactaaatc tttaccaaca atctgggcca ctgtatgctt
     2221 cgttaagtca tctttcgtag gacctaagcc acctgttaaa ataacagtat catatttttc
     2281 tagcgcatga cgtacacttg attctaaacg ttttttatta tctccaataa ctttatgttc
     2341 taatacattt tgtccaattt cattaaatac tttagataga aattgtccgt tggtattagc
     2401 gatttgacct agcaatagtt ctgagcctac agcaataatg gcaattgaca tttatatccc
     2461 ccttgtatcg tttaattcat tttttcatgt atttcattta aaaattcaca tcaattttag
     2521 attcaatgta tcagatgcta ctacagattt tccctaaaaa aatgaacgaa tattgtttta
     2581 caatatatca ttataaacaa ttgcgtggct aatt
//
LOCUS       DBNENW010000222         2573 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNENW010000222 DBNENW010000000
VERSION     DBNENW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.226"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(87..1223)
                     /locus_tag="AC1LW8_002615"
     CDS             complement(87..1223)
                     /locus_tag="AC1LW8_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIP4774366.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLISKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQHVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGTNKQS"
     gene            complement(1255..1884)
                     /locus_tag="AC1LW8_002616"
     CDS             complement(1255..1884)
                     /locus_tag="AC1LW8_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIP4774367.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="AC1LW8_002617"
     CDS             complement(1903..2172)
                     /locus_tag="AC1LW8_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIP4774368.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..>2573
                     /gene="msaA"
                     /locus_tag="AC1LW8_002618"
     CDS             2335..>2573
                     /gene="msaA"
                     /locus_tag="AC1LW8_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein MsaA"
                     /protein_id="HIP4774369.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEED"
BASE COUNT          764 a          406 c          404 g          999 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcgttccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag gtcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacatgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgatattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aattatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga ttt
//
LOCUS       DBNENW010000223         2572 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.227, whole genome shotgun sequence.
ACCESSION   DBNENW010000223 DBNENW010000000
VERSION     DBNENW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.227"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            28..2016
                     /gene="tkt"
                     /locus_tag="AC1LW8_002619"
     CDS             28..2016
                     /gene="tkt"
                     /locus_tag="AC1LW8_002619"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIP4774370.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2294..2536
                     /locus_tag="AC1LW8_002620"
     CDS             2294..2536
                     /locus_tag="AC1LW8_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIP4774371.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
BASE COUNT          899 a          367 c          518 g          788 t
ORIGIN      
        1 aaaattgaag aagggaagat acacaagatg tttaatgaaa aagatcaatt agctgttgat
       61 acgctacgtg cactaagtat cgacacaatc gaaaaagcga attctggtca tccaggatta
      121 cctatgggag ctgccccaat ggcttacact ttgtggacac gtcatctgaa ttttaatcca
      181 caatctaaag attacttcaa tagagaccgt ttcgtattat ctgcagggca tggttcagca
      241 ttattgtata gcttgttaca tgtttctggt agtttagaat tagaagaatt aaagcaattt
      301 agacaatggg gttctaaaac accaggtcat cctgaataca gacatacaga tggtgtagaa
      361 gttactaccg gaccacttgg acaaggtttt gctatgtcag taggattagc tttagcagaa
      421 gatcacctag cagggaaatt taataaagaa ggatataatg ttgtagatca ttacacatat
      481 gtattagctt ctgacggtga tttaatggaa ggtatatcgc atgaagcagc ttcatttgct
      541 ggacataata aattaagtaa attagttgtt ttatacgatt caaatgatat ttcattagat
      601 ggcgaattaa acaaagcttt ttctgaaaac acaaaagctc gttttgaagc atatggttgg
      661 aattacttac tagttaaaga tggtaatgat ttagaagaaa ttgataaagc gattactaca
      721 gctaaatctc aagaaggacc aacgattatt gaagttaaaa caacaatcgg atttggttca
      781 ccgaataaag caggaactaa tggtgttcat ggggcacctt taggtgaagt tgaaagaaaa
      841 ttaacattcg aaaattacgg tttagatcct gaaaaacgtt ttaatgtttc agaagaggta
      901 tacgaaattt tccaaaatac tatgttaaaa cgtgctaatg aagatgaatc tcaatggaat
      961 tcattattag aaaaatatgc agaaacatat cctgaattag cagaagaatt taaattagcg
     1021 attagtggta aattgcctaa aaattataag gatgaattac cacgttttga actgggtcat
     1081 aatggtgcat ctcgtgctga ttctggtact gttattcaag caatcagtaa aactgtccct
     1141 tcattctttg gtggatcagc agaccttgct ggttcaaaca aatccaatgt aaatgatgca
     1201 acagattata gttctgaaac acctgaaggt aaaaatgtgt ggtttggtgt acgtgaattt
     1261 gctatgggtg ctgctgtaaa tggtatggct gcacatggag gtttacatcc atatggtgca
     1321 acattcttcg tatttagtga ttatttaaaa ccagcgttac gtttatcatc aattatggga
     1381 ttaaatgcaa cgttcatctt cacacatgat tcaattgcag taggtgaaga tggtcctact
     1441 catgaaccaa ttgaacaatt agctggatta agagctattc caaatatgaa tgttatccgt
     1501 cctgctgatg gtaatgaaac aagagtagca tgggaagttg ccttagaatc tgaatctaca
     1561 cctacttcat tagtattgac acgtcaaaac ttaccggttt tagatgtacc agaagatgta
     1621 gttgaagaag gcgttcgaaa aggtgcctat acagtttatg gctctgaaga gacaccagaa
     1681 ttcctattat tagcttcagg ttcagaagtt agtcttgcag ttgaagctgc taaagatctt
     1741 gaaaaacaag gtaaatcagt gcgtgttgtt tcaatgccta actggaatgc atttgaacaa
     1801 caatctgaag aatataaaga atcagttatt ccatcaagcg taacaaaacg tgttgcgatt
     1861 gaaatggctt caccgcttgg atggcataaa tatgtaggta ctgcaggtaa agttattgct
     1921 attgacggct ttggcgcaag tgcacctggc gatttagtag ttgaaaaata tggatttact
     1981 aaagaaaata tcttaaacca agttatgagc ttataagaat aatttataaa gcgagtatgt
     2041 ttagaagtct aggatgcata atcttaggct tcttttaaag tgttgaaatt tagagtatag
     2101 cacttaaact acatcataag tgataagtta tgaaagtata ctatttcaga ttaatcttta
     2161 aaagctctgt tataacagca tgatttttga tattattttt agtatcgata ttaaaatact
     2221 tgaataaact agttcttgaa ataatgtgat gaatttagta aaattcagta ggataaagaa
     2281 agcgggtgaa acaatggcaa cttggttagc aattattttt atagtagctg cattaatttt
     2341 aggtttaatt ggaggtttcc ttttagctag aaaatatatg atggactact tgaagaaaaa
     2401 cccaccaatc aacgaagaaa tgcttcgtat gatgatgatg caaatgggtc aaaaaccttc
     2461 tcagaagaaa attaatcaaa tgatgacgat gatgaataaa aatatggatc aaaatatgaa
     2521 gagtgcgaaa aagtaaattc gcaattgata gaggctattt tccagatatg ga
//
LOCUS       DBNENW010000224         2555 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.228, whole genome shotgun sequence.
ACCESSION   DBNENW010000224 DBNENW010000000
VERSION     DBNENW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.228"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            7..903
                     /locus_tag="AC1LW8_002621"
     CDS             7..903
                     /locus_tag="AC1LW8_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HIP4774372.1"
                     /translation="MGSVLTTTLILGGCSAMENESNKDTNTETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDKEFKE
                     GMQQARKELDYTANTDAVSTLFSTKKNFTKDNTVDDVIELSNKLKEKPNMPQKSQVTI
                     QLGKSSINTKKPFYDDINPIEG"
     gene            915..2555
                     /locus_tag="AC1LW8_002622"
                     /pseudo
     CDS             915..2555
                     /locus_tag="AC1LW8_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1080 a          299 c          472 g          704 t
ORIGIN      
        1 aagttcattg gatcagtttt aaccacgaca ttaattttag ggggatgttc cgcgatggaa
       61 aatgaatcaa ataaagacac aaacacagaa acaaaatctg taccagaaga aatggaagct
      121 tcaaaatatg taggccaagg cttccaaccg cctgcagaaa aagatgcgat tgaatttgcg
      181 aagaagcatc gtaaagaatt tgaaaaagta ggtgaacaat tctttaaaga taactttgga
      241 ctaaaagtta aagctacaaa tgttgtaggt aaagatgatg gtgtagaagt ttatgtgcat
      301 tgtgaagatc atggcattgt atttaatgca agtctacctt tgtacaaaga tgccatccat
      361 caaaaaggat caatgcgcag taatgacaat ggtgatgata tgagtatgat ggtgggtaca
      421 gtgctgagtg gctttgaata tcgagcgcaa aaagaaaagt atgataactt atataaattc
      481 ttcaaagaaa atgaaaagaa atatcaatat acaggcttta caaaagaggc aattaacaag
      541 acacaaaacg ttggatatca aaatgaatac ttttatatca catatttatc aagaaactta
      601 aaagaatatc gtaaatatta cgaaccgttg attcataaaa atgataaaga atttaaagaa
      661 ggtatgcaac aagctagaaa agagttagat tatactgcca atactgatgc agtttctaca
      721 cttttttcaa ctaagaaaaa ctttactaaa gacaatacag tagatgatgt gattgaattg
      781 agtaataaat taaaagaaaa acccaatatg cctcaaaaat ctcaagttac tattcaatta
      841 ggaaaatcta gtataaatac taaaaaacca ttttatgacg atataaatcc aattgaaggg
      901 taagtgaata taaagtgaat aaaataaatg atagggacat agcagaattg agcagttatt
      961 gggtttatca agatataaat gaaaaaaaga ttttaaagta aatggaaaac ggttcaaaca
     1021 aatagataat tataatgata atagtaatga taatatgaat ggtgctgctg atattaaaac
     1081 atacgagtta cttaatgatc aggggaatcc aactggtcaa cagacaataa tttatcaagg
     1141 aacatctaat gaggcaatta acccaaataa tccattaaaa tcatcgggtt ttggagatga
     1201 ttggctccaa aatgctaaat taatgaataa tgataatgaa agcacagatt atttaaagca
     1261 agtagaagaa ttcgcaaata gttatcgaca aaaaattaaa gatgcttctg ttcttgatga
     1321 aaatgaattt agaagaaaat acaaaactaa accttataat tacaaaaaca aaacaattgt
     1381 ggcggatggt ggtaattcgg aaggtggtgc aggagcaaaa tatcaaggag cgaaacatcc
     1441 gaatgaaaaa gttgttgcta ctgactcagc aatgattcct tatgctgctt ggcagaaatt
     1501 tgctagacca cgctttgata atatgattag ttttaatagt accaacgatt tattaacatg
     1561 gttacaagat ccattcatca aagatatgcc aggaaaacgc gttaacatta gtgatggtgt
     1621 gcccaggtta gatgctttaa tagacagcca tgtaggttat aaaaggaagt taaatagaaa
     1681 agataacaca tacgatactg taccactaat caaaattaag tcggtaaaag atacagaaat
     1741 taaaaatgga aaaaaagtaa aaaagactat taacataaca ttagatatgg atgggcgaat
     1801 tccaataaat gtttggacag gagattcgat tgcacgttcc ggaaaaggaa ctttaattaa
     1861 acttaattta gaaaatcttg atgcgttgag taaactgatt actggtgaaa caagtggtat
     1921 gttagcagaa tgcgtaatct ttttaaatga aagttttaac atctcagaaa atgaaaataa
     1981 aaattttgca gatagaaaga aacaattatc agaaggattt aaggataaga ttaacttatt
     2041 tcaattagaa gaaatggaaa gaactttaat tagtaaaata aacgcacttg aagaagttgc
     2101 agatgaaaca atagaaagta ttagtgctgt taaacactta ttacctgatt ttgcattgga
     2161 tgcattaaaa gaaagaatta atgagttgtt taaaggtata aaatctttta tagaaaaagt
     2221 gtatgatagt atagataatg aaattttaga aattttcaaa aatatagatc acgacttcag
     2281 agatggagta tctgaagaaa tgatgaaaca tttgaaagta gtgaaacaga atatagagca
     2341 aataaaaaat caaaatgata tttatggtag gcaaattgca gatattagaa gtattatgaa
     2401 gcaacaagat gcaacaattt tagatggaaa ttatcaaatt aattatagcg gtgaaaatat
     2461 ggtgcagggt ctagctttac cttctaatta tttaggaaga aaaatgaaaa tattaaaaga
     2521 ccatatcgat gatggtatta aaaaaatagc agact
//
LOCUS       DBNENW010000225         2553 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNENW010000225 DBNENW010000000
VERSION     DBNENW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.229"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..2219
                     /locus_tag="AC1LW8_002623"
     CDS             <1..2219
                     /locus_tag="AC1LW8_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HIP4774373.1"
                     /translation="KKIREIQKLILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQI
                     DTKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNI
                     KDLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEI
                     NHYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKL
                     EHEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYEL
                     TLDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIEP
                     MNDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNI
                     VNLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKK
                     SSYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIE
                     KYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFF
                     RYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFSISNEERME
                     IVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNE
                     GLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            2212..>2553
                     /locus_tag="AC1LW8_002624"
     CDS             2212..>2553
                     /locus_tag="AC1LW8_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HIP4774374.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGH"
BASE COUNT          980 a          321 c          405 g          847 t
ORIGIN      
        1 tgaaaaaaat tagagaaatc cagaaattga tattagctta tgaaaaaact gaaattggat
       61 ttggcgagga attatataaa gacattattc atcatatgaa ggcactattc aaatgtaaaa
      121 actatcttca aattgatacc aaaattgaca tgattaacaa ttatttacat caagatatag
      181 caacaaatat atctgaagca gcgtacttat tgtggctttt atcaagaaat aatattggat
      241 tcacagattt gaaagttctt cacaaccgtt ttattgaaaa gtatggattt gaacaactcg
      301 tgaatattaa agatttgatt tcagatataa caggtttcgg gacaacaatt tttcaagaag
      361 aagaaaccga tggaaataat attgtaatgc ttaagcaaaa atttttacat gccttgagaa
      421 ataacgatga gatagtcata aatgaaaaag atgtagagtc attgattaac gataatgaaa
      481 tcaatcatta tcatgcgcca atgagtgcag atgtttatgc tgaactttat ttaggacgtt
      541 tttacaatca gtacaatgag ctcattgtta ttagtccttt aacggcgtct tttaatgctg
      601 gcgctacatt tgggcgattt catcatttaa ttgatactga aactttagca aaattagaac
      661 atgaaaaagg acattattat cagaagatga tatgtgatga caatgtagaa atgatttcta
      721 taaataacat accgaaatat ccgagaaatc ataatgtatt aactaatcat gactcatacg
      781 aatattcatt gaatttagga agtagtaata gttattcaaa gtatgagctt accttagatg
      841 atatttatgt tggtgctacc tttaacaaat tatatttata ttctagccaa ctaaataaaa
      901 gggtactatt tgaatcaaac aatatgtata actttttaaa agaatgtaat ttatatcgat
      961 tgttacgaga aatttcaatg gaatctgtga aatgcattga acctatgaat gatgtgagta
     1021 ttgattcatt tagttattca ccacgaatca gatataaaaa tgtcattctt aaaccagcat
     1081 attggaaaat taacgagatg gtattacctt tacctaaaaa tgaagaatgg gatcaacaat
     1141 ttttgaaata tcaagaacaa ttcaatatac ctaatatcgt gaatttagtt tatggagata
     1201 acaagcttct actaaattta tcattagcta atcatagata tttattgatg aaagaatata
     1261 agaagcataa acgtgtacgt ttagtagaat catttttacc tcaatcaaag aatgatcatg
     1321 tgtatgaaat tgttacacca atttataaaa aatcatctta tcgaggaccc gaaatcgaaa
     1381 ttccaaaata taaaaataca gatattgaat atgacaaaga ctggtttgca ttacatattc
     1441 atatcgataa gccatcgcaa gatacattta tcatcgacaa tttgtatcca tttgtaaaac
     1501 atcttaaaga taagggagat atagaaaaat attttttaat gcgttatatc aaacaaggtg
     1561 acattttaaa gttgaggtta ttcaggaatg acgaaaatta cgcagaaata tacagcatct
     1621 tgaagaattg gttaccacat gttcgacaaa cgacagaagt ttcagattat gaatttgtat
     1681 catacgagcc agaatttttt agatatggtg gaaagaacac gattaatgaa atagaagcct
     1741 tctttgaata tgacacgaat ttagctgtca atattattga aaatgatttt aagtttgatc
     1801 gaccatatat tgttgctatt tcaataatgt atttatttga gatgttttca atttctaatg
     1861 aagagcgtat ggaaattgtt aataactatg tacctacatc atttaaaagt aaagatatta
     1921 gaccttttaa aaatgaactt gtaactatat gtaatcctgc taataatttt gaatatatgg
     1981 ctaaacacta tagtggtatt tatcgaattt taaaagatgg caaccaaatt ttgagcaaat
     2041 taaatgaagg gcttaaaaaa actttaacta ctaaaaggtc aaggattatt ggcagcttaa
     2101 ttcatatgcg ctgcaatcga atatttggca ttgataagga tcaagaaaca tttgttttat
     2161 ctattgtaaa agaaattgtt aaaactcaaa agcattggtg tggtgacaaa aatgattgat
     2221 atgaatctaa tattgaagaa gaagtttcaa gagatttcag aagtagatga tttcatttca
     2281 aaagcatcta ctgaaaccga ttattttgaa ccttcaactt tatcacatgg tataccaggt
     2341 atcattttgt ttttagatgc ttatcaaaaa gttttcgata ttaatactga gcaaattgtg
     2401 cataagtaca tcatgaaatt agcaccatat ttacagagtg gtcaatataa caattctttg
     2461 tttggtggac tttcaggtat tgcattttca atggatttag catcgcaaaa tggcaaaaat
     2521 tatcaaaata ttttaaataa tattgatggt cac
//
LOCUS       DBNENW010000226         2521 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNENW010000226 DBNENW010000000
VERSION     DBNENW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.230"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..657
                     /gene="queC"
                     /locus_tag="AC1LW8_002625"
     CDS             <1..657
                     /gene="queC"
                     /locus_tag="AC1LW8_002625"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIP4774375.1"
                     /translation="LNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQRHDTE
                     IEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARNLLFL
                     SFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHTPLMW
                     LNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLESKGAL
                     "
     gene            659..1078
                     /gene="queD"
                     /locus_tag="AC1LW8_002626"
     CDS             659..1078
                     /gene="queD"
                     /locus_tag="AC1LW8_002626"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HIP4774376.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1082..1795
                     /gene="queE"
                     /locus_tag="AC1LW8_002627"
     CDS             1082..1795
                     /gene="queE"
                     /locus_tag="AC1LW8_002627"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIP4774377.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            1894..2478
                     /locus_tag="AC1LW8_002628"
     CDS             1894..2478
                     /locus_tag="AC1LW8_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774378.1"
                     /translation="MILYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
BASE COUNT          888 a          399 c          408 g          826 t
ORIGIN      
        1 ttaaataatg aaaaagccat cgttgtcttt agtggtggtc aagacagtac aacatgtctc
       61 ttttatgcaa aaaaacattt caaagaagtt gaactcgtaa cgtttaatta tggccaaaga
      121 catgatactg aaattgaagt cgcaaaacaa attgcacaag atcaaggaat gaaacatcac
      181 gttttagata tgtcattatt atcacaactt actccaaacg cattaacaca acatgatatg
      241 gaaattacta ataatgaaga tggtatacct aatacatttg ttccagctag aaatttactt
      301 ttcttgtcgt ttgcaggcgc tctagcttat caaattgggg ctaagcatat tattacaggc
      361 gtatgtgaaa cagacttttc aggctaccca gactgtcgcg atagttttat aaaatcaatg
      421 aacgtaacat taagcctagc tatggacaaa gattttgtca ttcatactcc tttaatgtgg
      481 ttaaacaaag cagaaacgtg gaaattaagt gatgaactcg aagttttaga ttatattcgt
      541 acaaaaacat taacatgcta taacggtatc attggggatg gctgtggtga atgtccagct
      601 tgtcatttac gtcaacgtgg actaaatcaa tatcttgaaa gtaaaggagc gctttaaaat
      661 gttacaacaa atctatccta gtacaacgca tccatatcaa ttcgaattaa ataaagactt
      721 taatttttcg gctgcacatc acattccttg tgaagaagca ggtatttgtc aaaatgtcca
      781 tggtcatact tactttgtta atttaacaat tgtcggtgat aaactagatg acactggctt
      841 cttagtgaac tttagccatt tgaaaaagat gatacacggt aaatttgacc atcaactgtt
      901 aaataactta cctgctttta aaaacaaaat cccttcaact gaaatcgtag cggaaacaat
      961 ttatcaaatt gttaaagaaa atttggcatc gctcgaacac caaccaaaat gtattcaagt
     1021 atttgtaaga gaaacaccaa caagttatgt tgtatttaga ccaaaggaac aggtgtaact
     1081 aatggctaaa attcctgtac tcgaaatatt cggtccaacc attcagggtg aaggtcgagt
     1141 aatcggtaga aaaacgatgt ttgtgagaac agctggatgt gattatcgct gtagctggtg
     1201 tgattctgca tttacttggg atggtagtgc taaaggcgat ataaaactca tgaccgctga
     1261 agaaatttat gatgaattaa aacgaattgg tggcgattta tttaatcacg ttacaatttc
     1321 tggtggtaac ccagcattaa ttaaaggtat ccaagagtta gttgacttat ttcaagataa
     1381 aggtattttc agcgcactgg agacacaagg cagtaaattc caaccttgga tgacacaaat
     1441 tgatgattta acgattagtc caaaaccacc aagttcaact atgacacctg atttaaaaaa
     1501 gttagatgaa gtgattacac aatgcgtacc ttcctcatta aacttaaaag tagttgtatt
     1561 cgacgataaa gattatgatt ttgccaaaat gatacaccac cgttatccag atattccctt
     1621 ctatttacaa gttggtaacc catatttatc agacagcgta gataatcata ccgaaaagtt
     1681 gttagaacgc tacgagcagt tggtggattt agttatgcaa agtaatgata tgaatcacgt
     1741 ttatgtgtta ccacaacttc atacattact ttggagtaat aaaaaaggtg tataaaggat
     1801 aacttatttt actttttaca agtcatatct aatgaataac aatatattaa ttgatataat
     1861 agttgattga agtataacga acaggagtcc atcatgattt tatatatatt aattaatatt
     1921 gctatcgtcg tacttataac tggattcaat ttatataggc atcaaatgca acatttatca
     1981 ttaagtgcaa tgttgttatc tattacgata aacgccttta taaatacatt cataattgat
     2041 aaatataact ttattactct gtgtactata acgatgttta tcatttggac gatactacag
     2101 ttttacattg ataaaaaatt aaaacctgta tacattacag accaaaaatt tattgctatc
     2161 atattaacga ttgttgtcag tttaacgcaa cgtgttaccg acttttcaag tacacaatca
     2221 atatatatgt ctattccatt tttagcccct gcaattttca tcattggtgg tattatgttg
     2281 tttatttcaa catttaatag cttagatgaa actgctgaaa ataataacaa aataaagaaa
     2341 ctaatgatta aaggacttat cattattaac atttcattta tcgttatgat ggttttaaca
     2401 ccatattggt acttgtattt aatcgtctat cttattttct tgttgttttt attgtggcaa
     2461 aaggtttata aattttaata ccaaaactat aaaacacttc tgatattctt agttcaaaat
     2521 a
//
LOCUS       DBNENW010000227         2491 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNENW010000227 DBNENW010000000
VERSION     DBNENW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.231"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            81..1649
                     /locus_tag="AC1LW8_002629"
     CDS             81..1649
                     /locus_tag="AC1LW8_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIP4774379.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            1794..>2491
                     /locus_tag="AC1LW8_002630"
     CDS             1794..>2491
                     /locus_tag="AC1LW8_002630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIP4774380.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKP"
BASE COUNT          775 a          346 c          500 g          870 t
ORIGIN      
        1 agaatattct gaaaaatata ttgaagtgtc tcgtgcttaa tcgtataata tttttatgtt
       61 taaacaatag ggggaatctt atgattgaaa aattagtaac ctttttaaat gaggttgtat
      121 ggagtaagcc attagtttat ggtttgctaa ttactggtgt gctatttaca ttgcgtatgc
      181 gattttttca agttagacat tttaaagaaa tgattcgatt aatgtttcaa ggagagaagt
      241 ctcctaatgg tatttcaagt tttcaagcga tagccatgtc tttagcaggc agggttggta
      301 caggtaatat tgtcggtgta tctactgcaa tatttatagg aggacctggt gcagtatttt
      361 ggatgtggat tactgcgttt ttaggtgcaa gtagtgcttt tattgaatct acacttggtc
      421 aaatattcaa gagagttgaa aataatgaat accgtggtgg accagcgtat tatattgaat
      481 atggtattgg tggtaaattt ggtaaaattt acggaattat ctttgctatt gttacgatta
      541 tctcagtagg tctattgctt cctggtgtgc aatctaacgc tatagcaagt tctatgcata
      601 atgcgattca tgttccacaa tggttaatgg gtgctattgt tgtagttatt ttgggattaa
      661 ttatttttgg tggtgtacgt agtattgcca atgttgcaac agccgttgta ccatttatgg
      721 caattattta catactgatg gctgtcatta tcatttgtat caatatacaa gaagtgccag
      781 cgttatttgc attaattttc aaatcagcat ttggattaca atctgctttt ggtggtatcg
      841 ttggcgcaat gatagagatt ggtgttaaac gtggattata ctcaaatgag gctggtcaag
      901 gtacaggtcc acacgcagca gcggcagcag aagtatcaca tccaagtaaa caaggtctag
      961 tacaagcatt ttcagtttat attgatacat tatttgtatg tactgcaact gctctgatta
     1021 tacttatttc tggtacatat aatgtgactg atggtacggt taatgcgaat ggcacaccgc
     1081 atttaattaa agatggcggt atttatgttg aaaatgcaac aggtaaagat tattcaggta
     1141 ctgcgatgta tgcacaagcc ggcattgata aagcgttcca tggcagtggt tatcaatttg
     1201 atcctacttt ctctggcgta ggttcgtact ttattgcatt tgctttattc ttctttgcat
     1261 ttactacaat tttgtcgtac tactacatta cagaaacaaa tgttgcttat ttaacgcgta
     1321 atcaaaataa tcaagtttca tcgatattta ttaatattgc tcgtgtgatt attttgttcg
     1381 ctacatttta cggtgcagtt aaaacagctg atgtagcatg ggcattcggt gatttaggtg
     1441 taggtctaat ggcttggtta aatatcattg cgatttggat tttacataag cctgccgtaa
     1501 atgctttaaa agattatgaa attcaaaaga aacgtttagg aaacggttat aatgcagttt
     1561 atcaacctga tccgaataaa ttacctaatg ctgtcttttg gttgaagaca tatccagaac
     1621 gtttaaaaca agcacgtgcc aaaaagtaat ctacttttgt ttatagtata tgtagtgatc
     1681 atttgataaa aaaagaaaca gcattgataa ttttaggtgc tcagaaattt gaattttaaa
     1741 aatatagtgt ctcttggtac aataacaata caactactag ggggcacttt tttatgtcag
     1801 aatttaaaac tggtaagatt aataaacatg ttttatatag taatatttta aatagagatg
     1861 tcacgataag tatttattta ccagaatctt ataatcaact tgttaaatat aatgtcattc
     1921 tttgctttga cggattagat tttttacgtt tcgggagaat acaacgtaca tatgaatcgt
     1981 taatcaaaga agcgcgtatt gatgatgcga tcattgttgg attccattat gaagacgttg
     2041 ataagcgtag agaggaattt catccacaag gaagtcgttc tcatttaact attcaatcag
     2101 tcggtaaaga aatattgcca tttattgact cgacgttttc tacactgaaa gtaggtaatg
     2161 caaggttatt agtaggggat agtttagcgg gtagtattgc cttattaacg gcgttgacct
     2221 atccaacgat ttttagtcgt gtagcaatgt taagtccaca ttcagatgac aaagtattag
     2281 ataagctaaa tcaatgtgct aataaagaac aattgacaat ttggcatgtc attggtctag
     2341 atgaaaaaga ttttacttta ccaacaaatg gtaagcgtgc cgatttctta acaccgaata
     2401 gagaattagc tgaacaaatt aagaaatata atataactta ttattacgat gaatttgaag
     2461 gtggtcacca atggaaagat tggaaaccat t
//
LOCUS       DBNENW010000228         2486 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNENW010000228 DBNENW010000000
VERSION     DBNENW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.232"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(258..1466)
                     /gene="cozEb"
                     /locus_tag="AC1LW8_002631"
     CDS             complement(258..1466)
                     /gene="cozEb"
                     /locus_tag="AC1LW8_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozEb"
                     /protein_id="HIP4774381.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1459..1626)
                     /locus_tag="AC1LW8_002632"
     CDS             complement(1459..1626)
                     /locus_tag="AC1LW8_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774382.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1601..2452)
                     /gene="glcT"
                     /locus_tag="AC1LW8_002633"
     CDS             complement(1601..2452)
                     /gene="glcT"
                     /locus_tag="AC1LW8_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter transcription
                     antiterminator GlcT"
                     /protein_id="HIP4774383.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          901 a          412 c          311 g          862 t
ORIGIN      
        1 atattattaa ttaacagccg attatgagtg cttcttaata tattaaaaat accacttgtt
       61 tttattttcg acataataat gaagtactaa tcaagtggca aatactcgtt tcattatgca
      121 tgtgctttat aaacggctaa actttcgtaa aaatttataa gctaaacatg tgctgataat
      181 cataaatgta actgcccaac attataagca ccataaaaaa taccaacaaa catatagtca
      241 ttggtatttt ttattactta ttcaactatt ttattacttt ctttaatatc atattcacct
      301 acatcattac catagaaacg attgtatcga cgtttaaata attggaataa atgagtaact
      361 aatactttta aaatagcata tcccggaata cctaaaataa cgcctacaat accaagcaat
      421 ttgcctgcac acagtaaaat gaaaatgatt gtaagtggat gaatcttaag tgttttaccc
      481 atgatatttg gtgaaatgaa gtgcccttca acaaattgta ctaaagtcca tactactgct
      541 aattttaaga gcatccacgg cgatgttata gcagctatta caatagctgg agaaatcgct
      601 atagtaggcc ctaaatatgg tacaacactt gtaactgccg caatactagc taatactaag
      661 ctatatttca acccgataac cgaataaccg ataaacaaca gtataccgat acagaatgaa
      721 acgataattt gtccttgaat gtatgaacca acttgaacac tcattttttc aagtagatca
      781 tgaaaatctt ttcggaattt cggtggcata atattcgttg aaaattcttt gaagtgatgt
      841 ccatctttaa gcataaagaa tagtacaaat ggtgttgtga caatcacaac gccaatatta
      901 gcaatcgttt ctgcaaacgt tgcaatttta gacccaaagc catctgtaaa ttcacttaac
      961 atagatggta ttttcttagg taaagaattt aaccaatcat taatttgact ataatacgaa
     1021 gagaaaaacg tattatttgc tattttatca atagaattaa ttaatttttc tagatattgc
     1081 ggtgaatttt taactaaact atctacttgc gaaccaataa taggtatcaa taaattaaca
     1141 attaacgtaa taacacctac tacagctaga taaataatag aaatacctgc aactcttggt
     1201 attctataac gttccatcat atttacgatt gggttaaata gatagaatag aattaaagag
     1261 acgatgatag gtgctgctat cgtcttaaaa acgatgataa aaggatcaaa aacatatgat
     1321 actttttcga aaataaaaat aacaataccc aacaatacta gcgctattaa tgagaagact
     1381 aaatcattcc caccaacaaa cttcatatac cgtgattcgg taaattttaa aaaattcttt
     1441 cttatattct tttcattttc attcaattat agattcacca ccacttaaaa tattcataag
     1501 atgttgtata aaaacagtcc acttatgtaa aattactaac atcaatgata ccatacaaat
     1561 accctttaca gtaacgaaac gcccgtgtaa tacacttaat ttaggattga ttaatccttt
     1621 cttcaaaatg atgaatgtgt aatgttaaat atataatttc agactcactg atattaacat
     1681 caaattgttt ttgtatcata gttaaaattt tatatgctgt gttatagcaa atcggatagt
     1741 gatttttaat catagacaca aaatcatctt gtgcatgtat atattctttt cttcttaatc
     1801 ggcgaattaa aaattgtacg tgccttataa aacgttggta ttgtaatgat tccttatcaa
     1861 cagttgtcac aaggtctgat tcaatgatat ctataccttt tttaataaca ttattgatca
     1921 aggtcatctc atgcatagat aaatcttctg tattagatgc aatatgtaat gcaataaatc
     1981 ctatctcatc ttcaggaaaa tgtacatcta atgctgcatt taactgatca atcacctgtt
     2041 tagcaatatg atatgcatca ctatataact gcatagtttc cataacaaat ggattgctaa
     2101 taacttgatt ttgttttaag cgtttataag caaatataat atgatccgtt aatgaaacta
     2161 caagttgttt tgaatcaaca ttcatcgcag tattagaaat aaaattcaac gaatcaataa
     2221 ttacttgtaa tacattatca tcagcgattt caactaaact tttataatgt gctttttgtt
     2281 gctcactctc taatttataa attttctcta ttgtaatagt ttggtcgttt aacgccattc
     2341 cctctttttt gttaaaacca atacctttac cgattaaaat aacttcttga tcattattag
     2401 tacatactac gacattgttg ttcaatgttt tagtaacaat atattctccc attatcatca
     2461 cctatttttc tattattaag attata
//
LOCUS       DBNENW010000229         2479 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNENW010000229 DBNENW010000000
VERSION     DBNENW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.233"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            66..443
                     /locus_tag="AC1LW8_002634"
     CDS             66..443
                     /locus_tag="AC1LW8_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HIP4774384.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
     gene            631..813
                     /locus_tag="AC1LW8_002635"
                     /pseudo
     CDS             631..813
                     /locus_tag="AC1LW8_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(791..892)
                     /locus_tag="AC1LW8_002636"
     CDS             complement(791..892)
                     /locus_tag="AC1LW8_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774385.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(1015..1110)
                     /gene="sprA1"
                     /locus_tag="AC1LW8_002637"
     CDS             complement(1015..1110)
                     /gene="sprA1"
                     /locus_tag="AC1LW8_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     SprA1/PepA1"
                     /protein_id="HIP4774386.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1313..1456
                     /locus_tag="AC1LW8_002638"
     CDS             1313..1456
                     /locus_tag="AC1LW8_002638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIP4774387.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            2060..2443
                     /locus_tag="AC1LW8_002639"
                     /pseudo
     CDS             2060..2443
                     /locus_tag="AC1LW8_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          965 a          317 c          419 g          778 t
ORIGIN      
        1 aactcaatat gcttaattta ttcaaaatgt tcgtcaataa gtacaatgaa aaggagattg
       61 attttttgaa taaaaaatat cttatgattg taattataat tttaatattg attctaggcg
      121 gaatcttttt gaagatgaaa tatgacgaaa agaaatatta tgatgaacaa aaagaaagaa
      181 taacgattta tatgaagtac aatgtgaaag gttataaaaa tataagcttc gctaatttta
      241 aagaaaaccc aatggatggt tattctatta gtggttatat aaataatgat aaaaagttat
      301 catttacagc tggtataaga tctgttgatg attttcaatt tgataccgat atttcttata
      361 cagatgaatt gggtagaaaa tttaataaaa atcctaagtc agtttctgaa ataaaaaaag
      421 agcaaaatac gtccaataaa taattgttca tattgtgatg aaacaaaaat ataagtcatt
      481 agatgagttt aactatgtta taaatatttg tagtatctat aaaaatctcg acactattaa
      541 aatgataaag tgccgaggtt ttcttactta tttagttaat tcaaagttta tgccagattc
      601 ataagaattt gtgacacttt taatagtgta accattgatt attacaattt atcaaatggt
      661 cctttagaag gtataaataa caaaataaaa ttaattaaac gtacatcttt tggttatgga
      721 agttacaatc atttgcgcaa tcgaatatta ttatgttcaa aactttacgc tccaaaaagt
      781 aaaaaggaag ttaagcaatg tttagttgct taacttcgga tattgaacgc atcagtccaa
      841 tttgacatag agcctttttt agttcttgat gtttctcttt aaaaccttgc atattttaca
      901 aaaagaaaaa ttagcagtat aattaagaca acgaaaataa gtatttactt atacaccaat
      961 cccctcacta tttgcggtag tgaggggatt tttattggtg cggctatatg tcacctattt
     1021 tgtattgcgt ctacttagcc aataagaaaa aaacgcaatg gcacagccac tgatgactgg
     1081 tgctatgatg tgaacgaaaa taagcatcac cttatacacc tcctctctgc gtctaaattg
     1141 acgcctgaga gataggcgac tctactatta tatcatcggc aaatatacaa gcacagtcac
     1201 ttgcttctga taagttatat gattctagct gatagattga atcgtctaca cttaattgga
     1261 caaattctat gagaatagat attgttaatt taagaaagta ggcgatttta ttatgacaag
     1321 agaaagaaga tcatttagtt cagagtttaa gttacaaatg gttagattat ataaaaatgg
     1381 taagcctagg aatgaaatta tacgcgagta tgatttcaca ccttcgacgt ttgtaaatgg
     1441 cggttataaa atgtaggaaa atggataaag caacatcaaa acacgggtac attcaatcac
     1501 caagataact tatcggatga agaaaaagag ctgattaaat tacgcaaaga agttcaacat
     1561 ttaaaaatgg agaacgatat tttaaagcaa gtagcgctga ttatggggca aaaatagaag
     1621 tcattcaaaa gaatgcacat caatattcag tatcagcaat gtgtaaagtc ctgataatac
     1681 taagaagtac ctattatgat tctataaaaa gaaaagataa taaaatcact aaagatgatt
     1741 caaacataga acatgccgtc ataaatattt ttaattctaa tagaaaagtc tttggtacaa
     1801 gacgaattaa aaatcattta aatgacaagg gtctcactgt atctggacaa aagataggtc
     1861 gatcatgaaa aaatctagtt tctgtttata cgaaatctaa atacaaaaat catctaaaag
     1921 aaactaatga aaaacgaatt aaaaatcttt attattagct gctggtgtat tatttgttag
     1981 tccaatttca ttatcttata attcagatgt agctcatgct gaagataagt tagaccattc
     2041 tcaagcaaag gtaatatatt tgagtaacca aaatttattt gatgaacttg agaaaaaagg
     2101 ttataaactg gaagatatat ttacaaaaga agaaataaaa aaatataaag ctgaagacca
     2161 attaagagcg ggtaaaactc aatatgtaga aacaggtaaa gatactgcaa cattatatct
     2221 ttcttctgca tatacaaaaa caatagctgc tttaggtgcg ggggctatta gcgttatttc
     2281 tgcactaaca ggtggtttag ttggcgcagg tgttggtggt ttccttggat ctatagctgc
     2341 atcgaatatt gatactagta agggaatata tatataatta aaaactaaaa agaatgcagc
     2401 cggggaatac gttctgacag gagaaaaatg gggatatcag taagggtgat tttatggata
     2461 gattaaaata ttcacttaa
//
LOCUS       DBNENW010000230         2476 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.234, whole genome shotgun sequence.
ACCESSION   DBNENW010000230 DBNENW010000000
VERSION     DBNENW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.234"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..253
                     /gene="scrA"
                     /locus_tag="AC1LW8_002640"
     CDS             <1..253
                     /gene="scrA"
                     /locus_tag="AC1LW8_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SaeRS system activator ScrA"
                     /protein_id="HIP4774388.1"
                     /translation="QILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAHTLNSS
                     SAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            397..705
                     /locus_tag="AC1LW8_002641"
     CDS             397..705
                     /locus_tag="AC1LW8_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774389.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(813..1046)
                     /locus_tag="AC1LW8_002642"
     CDS             complement(813..1046)
                     /locus_tag="AC1LW8_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774390.1"
                     /translation="MLRSLCKHNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1027..2220)
                     /locus_tag="AC1LW8_002643"
     CDS             complement(1027..2220)
                     /locus_tag="AC1LW8_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774391.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNISMIDRHSKEDFSETWEFLKKSKE
                     IPFIIIIQFACILINTGLWRVALPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLVGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISLIIPLLSLSNIFISGGAIISMLSLAGLRF
                     VKKSM"
BASE COUNT          922 a          422 c          299 g          833 t
ORIGIN      
        1 acaaatactt ttgattatgg gcattatatc tcttattgtt ttatttattt ttacactatt
       61 catcatggcg caatatgcaa aacattatga acaaaaatcc gacagttcca acgcacatac
      121 tttaaattca tcatcagcca taattgagca acatactatg tcgaatttag cgtctctaga
      181 tttatacgct cctgttcgta acattacttc tagtcgtgat attcatccca tttatttcat
      241 gacaaaagat taaaacttcc tttttcataa ccccattcat tcaactaata aattaaaaat
      301 tgataaaaac gtactgtttt ttagaaacat acatatataa gaaacgcatc cattactaaa
      361 agacagtaca ttatattcta tcgggaaggt gcaacaatga ccgtcaacat cgtgttttct
      421 atcgtgttct gtatcagtat ggttatatta ggcatttatg tcgcaataac taaagatttc
      481 acactaatct cttttataaa tcaaacgacc attgcagata aacacaagaa ccaaattgca
      541 tatatattta cgctatgtat cagtttgagt gcagtatttt tgatgtcttc aattctaagc
      601 tttgaatatg attttattgc attagcattc ttatttttaa cgatagcatt attacttatc
      661 gcattattct acgtttgctt ttataaaatt acaaaatacc cgtaaatgtt tgtgatagtg
      721 tgttggttag atttattgat gcgctgtcag taagtaattg tttgcgaaaa aaatacaacc
      781 ttgtacgaat acaaagttgc attaatatag aatcatttat gctttttaat atcacaaaat
      841 aaattgatta aactagctaa aatagtcaaa attggaatca agattacata aatatcataa
      901 cctctagata ttagtataca taatataatt agcgacccta ttataattgc taaattataa
      961 gccaaactaa ttttatattt catttcaatt cccaccttaa tagcattgat taaaattcta
     1021 ttatgtttac atagacttct taacaaacct taacccagct aagctaagca ttgaaataat
     1081 tgcacctcct gatataaata tattacttaa gcttagtaaa ggtataatta aacttataag
     1141 tcctaaagac aatgtatccg ctgcgtaatt cgatgtagat gagatactaa atactttccc
     1201 catcaaatga tttggcgtct ttatttgaat ggcaactgat cttgttagtc cctctataga
     1261 ttgcccaagt cccactaaag ttgcacctat atatagtatc gccacacttg gaaacacatt
     1321 aattatcgat aagccaatcc cccaaactaa aacaccaata ctaaatttaa agattaatcg
     1381 tttttctgac agtaaaccca taatcaatga cattaataaa gacgctatac ctaaacatga
     1441 tgtagctagt ccatatacac caacgccctc ttttaatata ttggaaataa acaatggtaa
     1501 tgcaacacgc caaagaccag tattaatcaa tatgcaagca aattggatga tgatgataaa
     1561 tggaatttct ttagattttt tcaagaattc ccaagtttca gaaaaatctt cttttgagtg
     1621 tctatcaatc atgctgatat ttgtatattt taaaagtgca ttaaaaataa atcctaaaaa
     1681 tagcaatata ctgcaaataa aaaagacgcc aacattacca actagtatta caatgacacc
     1741 aattaaagca ggtaaaataa tatttgagcc tctttgcaaa ctatcgatta acgcattact
     1801 tgttgctaaa tgctcctcat caataatttc aggaagaatt gccctaaacg caggatccgt
     1861 atagcagtta ataatggtga tagctgtaga tatggttaga agcgtcagat aatttaaatt
     1921 tgatgttatt gcaagtaaag gaataattat tataatcaaa cttagtataa gatcagatag
     1981 ataaagtatt ttctttttat tatgtttatc agaatatgcg ccaccgaaaa taccaaatat
     2041 aataaatggt agtgtttgac tcataaccat cattgataat tttaaagatg attggtttgt
     2101 caattcaaca gtaaaccaaa ttatttgtaa cgaaaacagc acaaagcaac tccgacgtaa
     2161 gaaattacca atcaataaat atgtaaagtt tctatttttc aaaacttcta agtacaacat
     2221 atttatcacc tctcataaaa ataattgaat gcatccacca gcttttttag accttcttct
     2281 aaactctctt tatccaaagc gcaattaatt ctaatataat ttagtcagtt aaatatcaat
     2341 tatttcgaaa tttacatact acttgaaaca ccatacataa cccccaaaat gactactcag
     2401 aggttatatt ctactaatta tgattatatt aaatatgaaa atattatcaa aaaatcaaat
     2461 ttataacaaa aataca
//
LOCUS       DBNENW010000231         2460 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNENW010000231 DBNENW010000000
VERSION     DBNENW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.235"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..909
                     /locus_tag="AC1LW8_002644"
     CDS             <1..909
                     /locus_tag="AC1LW8_002644"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP4774392.1"
                     /translation="MFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVK
                     KTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAE
                     KNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAIN
                     KLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSM
                     LVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQ
                     HLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            906..1508
                     /locus_tag="AC1LW8_002645"
     CDS             906..1508
                     /locus_tag="AC1LW8_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP4774393.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1621..1809)
                     /locus_tag="AC1LW8_002646"
     CDS             complement(1621..1809)
                     /locus_tag="AC1LW8_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIP4774394.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            1948..>2460
                     /gene="nucI"
                     /locus_tag="AC1LW8_002647"
     CDS             1948..>2460
                     /gene="nucI"
                     /locus_tag="AC1LW8_002647"
                     /EC_number="3.1.31.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016894 - endonuclease activity, active
                     with either ribo- or deoxyribonucleic acids and producing
                     3'-phosphomonoesters [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease NucI"
                     /protein_id="HIP4774395.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQK"
BASE COUNT          963 a          281 c          428 g          788 t
ORIGIN      
        1 atgttttaca ttttgttgat tattcattat tttattattt gttattttgt tttcagtgta
       61 catccaatgc taagtttgtt tttcttttat agtgcttttg ccgttccatt tacttttaaa
      121 aataatgtta aaaaaacggc aactaatctt ttcatactaa caatgattat atgtacaata
      181 ataacgtact tattgtataa caactatttt gttgcaatga tggtttatta tgtcgttata
      241 tcgttaataa tgctagataa ttttaaaaaa atgaaaaacc gtgaatatca aaaagaaata
      301 gcagaaaaaa atagacatat taatacatta attgctgaac aagagcgaca tagaattggt
      361 caagacttac atgatacgtt agggcatgtg tttgcaagtt tatcattaaa atcagaatta
      421 gcttataaac taatagatgc tgatgtagaa aaagtaaaag ctgaattatt agcaattaat
      481 aaattatctc gtgaatcatt gaacaaagtt cgagaaatta ttgatgatgt aaaattacca
      541 tcatttattg aagagattga tagtatacgt aaagttttaa aagatgctga tattgatttt
      601 acatttgaaa ataaagaatt agcgcaagta ttaagtccta ctaaacaatc tatgttagtt
      661 atgattacgc gtgaagcgat aaataatgtt attaaacatg caaatgcttc aaaagttcat
      721 ggtaaattaa aaactgtaaa caatcataaa ttactgctta tgattgaaga tgatggcaaa
      781 ggtatcgata gtgattgtga ggtgaaaagt atttcacagc gtgtacaaca tttaaatgga
      841 actttagcag tcgactcaac aaatggaact aaaataatca ttgaaatctc aacaggagga
      901 atagcatgac atctttaatt attgcagaag atcaaaatat gttacgacag gcaatggttc
      961 aattaattaa actacatggt gattttgaaa ttttagcaga tactgataat ggtctcgatg
     1021 caatgaaact tattgaagaa tataatccta acgttgttat tttagatata gaaatgccag
     1081 gcatgactgg acttgaagtt ttagcggaaa ttagaaaaaa gcatttgaat attaaagtga
     1141 ttattgtaac aacttttaaa agaccgggat actttgaaaa agcagttgtg aatgatgtgg
     1201 atgcatatgt tttaaaagaa cgttctatag aagaattggt ggaaaccatt aataaagtaa
     1261 ataacggaga gaaagaatat agcgccacat tgatgacttc attttttgta gataaaaacc
     1321 cattaacgcc caaagaacaa attgtattaa gggaaattgg caatggttta agtagtaaag
     1381 aaataagtga aaaattattt ttgacagatg gaacagttag aaattataca tctgttataa
     1441 ttgataaatt atttgcagat aatcgttttg atgcttggaa aaaggcaaat gaaaaaggct
     1501 ggatctaaat acaaacaaaa aagtattgag tgttttgatt taagagactt ggaaagtgag
     1561 tcaataatca aaatcttact caatactttt attttggtac tcgcaattta gatttgttaa
     1621 ctaatttttt cgcttattat aaaacgaaac aatcaagtaa acgataaagc ctacaaagat
     1681 acccaataaa atagatagta ctgccgtcac tattaatggt aatttgaaaa atatttgtag
     1741 gaaaatacca atgataattg cgataattac tgcaattaat gtgactgtat tttcatttga
     1801 aatgttcatt tattttcact ccttaacaat aacattatat catgctatag ctttccaaaa
     1861 tattgaaata tgtagatatg gctattgacg atatttctta acttttatat gattaatcgg
     1921 aatgaaaaaa gagaagtagg tggcaatatg aagtcaaata aatcgcttgc tatgattgtg
     1981 gtagccatca ttattgtagg tgtattagca tttcaattta tgaatcatac gggtcctttc
     2041 aaaaagggga cgaatcatga aactgtacaa gatttaaatg gtaaagataa agtacatgtt
     2101 caaagagttg tggatggtga tacatttatt gcaaatcaaa atggtaaaga aattaaagtt
     2161 aggcttatag gggttgatac gccagaaacg gtgaaaccga atacgcctgt acaaccattt
     2221 ggcaaagaag catcaaatta tagtaagaag acattaacaa atcaagatgt ttatttagaa
     2281 tatgataaag aaaaacaaga tcgctatggt agaacattgg cgtatgtatg gataagtaaa
     2341 gatcgtatgt acaataagga attagtggaa aagggacttg ctagagagaa gtatttttca
     2401 ccaaatggca aatatagaaa tgtatttata gaagcacaaa ataaagctaa acaacagaaa
//
LOCUS       DBNENW010000232         2396 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNENW010000232 DBNENW010000000
VERSION     DBNENW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.236"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..2016
                     /locus_tag="AC1LW8_002648"
     CDS             <1..2016
                     /locus_tag="AC1LW8_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP4774396.1"
                     /translation="DDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTA
                     KLIKSSYNLKCLLLKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRY
                     AYYGDLRDELEYIYQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKI
                     GKSIEILLTTDSTISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKP
                     EPLVGKMVQEVKEIILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLV
                     FLEGIFKTLLNKNSQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIE
                     TTYFVEEAFMSVFRQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVK
                     LGLNITCLFEKPSVFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFK
                     NLKQVINELDIEQEKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLN
                     IFDNHHTFNAMHLYDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIY
                     DWRVIESETIMSNFEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKY
                     EWNPSLLSKIDNYLKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2162..>2396
                     /gene="sarX"
                     /locus_tag="AC1LW8_002649"
     CDS             2162..>2396
                     /gene="sarX"
                     /locus_tag="AC1LW8_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HIP4774397.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIE"
BASE COUNT          959 a          274 c          355 g          808 t
ORIGIN      
        1 gatgatatat atattattaa tcatctggat gtatttagta ttaagaataa taaaaaaacg
       61 atcatgttgt atttgagtag cgattggttt gcggaattag gctttacttt ctttaattac
      121 cactatacag caaagttgat taaatcatcc tataatttga aatgtctact attaaaattg
      181 acatatcgat accttgataa tcagcctctt aatgacgctg atattagaaa attacaggat
      241 attattaaaa tcattgcaaa agaagcaagt atggataaaa agattgcaca aaatcaatat
      301 cgatatgctt attatggtga tttgcgtgat gagctcgaat atatttatca aaatgtaaat
      361 caacgattga cattaaaaag tgtcgctgat aaattatttg tctcaaagtc aaatttgtca
      421 tcacaattcc acttacttat gggcatgggt tttaaaaaat atattgatac tttgaaaatt
      481 ggtaaatcga ttgaaattct acttactact gatagtacta ttagcaacat aagtgaacat
      541 ttaggtttta gtagtagctc cacttactct aaaatgttta aaagttatat ggatataaca
      601 ccgaatgaat atcgtaattt atcaaaatat aataaatgtt taatgctaaa gccagaacca
      661 ctagtaggca aaatggtgca agaagtaaaa gaaatcatat tgaattatat tgaacattat
      721 aaaaaccacc taactgatgt tatacatatt gatgaagaca aatttgaaac acctaaattg
      781 tttcaaacgg ttattcaaat aaatacttat acagaaatga aattagtttt cttagaagga
      841 atctttaaaa ccttattgaa taagaacagt caagttgtct ttttcatcat gccatcgatt
      901 ctaaaaagta aaaataccat gtccgaagaa gaaaaattca caatcattaa aacaataatt
      961 gaaagtgatc taaagatagc atttaatata aatgatattg aaacaactta ttttgttgaa
     1021 gaagctttta tgagtgtttt cagacaaata tctccaaacg aattaagtaa tcataataat
     1081 tacgaagtgc attttgtttt tgatttatca ttgatggaaa ttagaacaat ttatcgaatg
     1141 atattaaaat tacataacat catgttgaat gtgaaattag gattgaacat tacctgttta
     1201 tttgaaaaac cttcagtttt taaatcacta gtatcacaaa taaagcgact taaattcgat
     1261 tcgttaataa tagataatgc aaatttaagt agcccttatt tgatggggga aagtgatgag
     1321 ttactattga aaaatatttt gcattttaaa aatttaaaac aagtaattaa tgaattggat
     1381 attgaacaag aaaagcttat ttttctaaat gttgaaaatc ataaactgct taataataaa
     1441 gaacgagatt taagtaatag tgctccatta atttataaga cattaagtgc gctgtatcac
     1501 aactttgatg gctttggatt aaacattttt gataatcatc atacatttaa tgcgatgcat
     1561 ctatatgata aaaatggatt taaaacaaca ctaggtctta tattggaaaa atttatcgaa
     1621 tatgtctcga aaccaaaata cgaaaacagt tattattcta tttttgatat agagaattat
     1681 tattgtcttg ttatttatga ttggcgagtg atagagagcg agacaattat gagtaatttt
     1741 gaggatagtc aagtttatat aaattttaaa aacaatgttt taaacgataa atatctaatt
     1801 gtaatagaaa cattggacga aaatagtggc aacattaatc atttgatttc taaagaatta
     1861 agagataaat atgaatggaa ccctagttta ctatctaaaa ttgacaacta ccttaaacca
     1921 gcaatagaga ttaaagagca taattttagt gataattcct tgaatattaa cgttactttt
     1981 aatgcgttat acataattaa aataggaaaa aaataacacc ttgatatgta ttgcaaaatt
     2041 taatttgcat ttgttggtaa ttttgacaac gtattaaaaa caaatgagaa agacaatggc
     2101 aaaaaaattt aagataataa tgacagaagc attgtcttta tatatttggg ggtgcaacat
     2161 tttgaatact gagaaattag aaacattgct tggcttctat aaacaatata aagcattatc
     2221 tgaatatatt gataaaaaat ataagttgtc gctaaatgat ttagcagtct tagatttaac
     2281 gatgaagcat tgcaaagatg aaaaagtact tatgcaatca tttttaaaaa ctgcaatgga
     2341 tgagctagat ttaagtagga caaaattatt agtttctata agaagactaa ttgaaa
//
LOCUS       DBNENW010000233         2396 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNENW010000233 DBNENW010000000
VERSION     DBNENW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.237"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            100..2379
                     /gene="secDF"
                     /locus_tag="AC1LW8_002650"
     CDS             100..2379
                     /gene="secDF"
                     /locus_tag="AC1LW8_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HIP4774398.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT          851 a          337 c          434 g          774 t
ORIGIN      
        1 tatgtataag gctaataaat tttgtaagaa agatttaact tgcgttataa aatagtattc
       61 aagtattcta aatacatacg taaataatga ggtgttcatg tgaagaaaag tagtagaata
      121 attgcgttct tgcttttagt cgtattgttg tttgccggaa tggctgcaac ttataaaagt
      181 gttataaaga atgtcaattt aggacttgat ttgcaaggtg gatttgaggt gctttatcaa
      241 gtcgatcctt taaataaagg cgacaaaata gataaaaaag ccttacaatc aacagcgcaa
      301 acattagaaa atcgtgttaa cgttttaggt gtttcggaac ctaaaattca agttgaagaa
      361 cctaatagaa ttagagtaca acttgctggt gtaacagacc agaatgaagc acgtaaaatt
      421 ttatcttctc aagctaattt aacaattcgc gatgcagaag ataaagtaaa attaagcggt
      481 tctgatatta aacagggatc tgccaaacaa gaatttaaac aggaaacaaa ccagcctaca
      541 gttactttta aagtaaaaga caaaaacaaa tttaaaaaag taacggaaga aatctctaag
      601 aaacgtgata atgtcatggt tgtttggtta gatttcaaaa aaggtgacag ctataaaaaa
      661 gaagcacaaa agaaaaaccc taaatttatt tctgcagcaa gtgttgatca accaatcaat
      721 tctgatagcg tagaaatctc gggtggattt aaaggacaag aaggcgttaa aaaagcaaaa
      781 caaatcgccg aattgttaaa tgcaggttct ttaccagttg acttgaaaga aatttattca
      841 aactcagtag gtgcccaatt tggtcaagat gcattagata aaactgtgtt tgcatcgttt
      901 attggtgtag cattaattta tttattcatg cttggattct accgattacc tggtttagta
      961 gcgattattg cattgacaac ttatatctat ctaacgttag tagcatttaa tttcatttcc
     1021 ggggttctaa ctttaccagg attagcggcg ttggtattag gtgtaggtat ggctgtagat
     1081 gccaatatta tcatgtatga gcgtattaag gatgaacttc gaataggtag aacgataaag
     1141 caagcctttt ctaaagcaaa caaaagttca ttcctaacaa tttttgattc taacttaaca
     1201 acagttatcg ccgcagcagt attattcttc ttcggtgaaa gttcagttaa aggtttcgcg
     1261 acaatgttat tattaggtat tctaatgatc tttgttacag ccgtgttctt atcaagattc
     1321 ttattatcat tacttgtttc atcaaatata ttcaaaaatc aattttggtt atttggtgtt
     1381 aaaaagaata aacgacatga tattaatgaa ggtgtagatg ttcatgacct taaaacttca
     1441 ttcgagaaat ggaattttgt taaattagct aagccattaa ttggagtaag tattttaatt
     1501 gtagttgtcg gtttagttat tctttatatc ttcaagttaa acttaggaat tgatttctca
     1561 agtggtacac gcgtagattt ccaatctaaa caagcaatta ctcaacaaaa ggtagagcaa
     1621 gttgtaaaag atagtggact taaagcggat caaattcaga tcaatggtaa agacaataaa
     1681 gtagcaactg ttcaatttaa agacgattta acgcgtgctc aagataataa attgagtgat
     1741 aatattaaat cgaaattcgg tgacacacct caaattaata ctgtatcacc aattattgga
     1801 caggaattag caaaaaatgc aatgcttgca ttaatctatg catcgatagg tatcatcatc
     1861 tatgtatcat tacgatttga atggcgaatg ggtctttcat ctgtattggc attattacat
     1921 gatgtattca ttatagtagc gattttcagt ttatttagaa ttgaagtaga tttaacattt
     1981 atcgccgctg tattaacaat tgtcggttat tcaattaatg atacaatcgt aacgtttgac
     2041 cgtgtacgtg aaaacttaca aaaggttaaa gtgattacga caacagaaca aattgatgat
     2101 atcgttaata gatcaattag acagacaatg acacgttcaa ttaatacagt attaacagtt
     2161 attgtagtag tagttgctat actattcttc ggtgctccta cgatattcaa ctttacttta
     2221 gcattattta tcggattgat ttctggtgta ttctcttcaa tcttcattgc cgttccgcta
     2281 tggggaataa tgaaaaaacg tcagctgaaa aaatcgccga aacacaaatt agttgtatat
     2341 aaagaaaaga aatcgaacga tgaaaagatt ttagtttaaa atgaattaag cggtat
//
LOCUS       DBNENW010000234         2361 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNENW010000234 DBNENW010000000
VERSION     DBNENW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.238"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            89..622
                     /locus_tag="AC1LW8_002651"
                     /pseudo
     CDS             89..622
                     /locus_tag="AC1LW8_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(735..2225)
                     /locus_tag="AC1LW8_002652"
     CDS             complement(735..2225)
                     /locus_tag="AC1LW8_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIP4774399.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNETTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT          731 a          411 c          306 g          913 t
ORIGIN      
        1 ggtcttcgac tggcactgct ccctcaggag tctcgccatt aatactacgt attaacatgt
       61 aattttactt ttaaatactt taaaaaaaat aagacacttt gcccaactta cactaccaat
      121 agaaacttct gttagaattc ctcaaaatga tatttcacga tatgttaatg aaattgttga
      181 aacgatacct gatagcgaat tcgatgaatt cagacatcat cgtggcgcaa catcctatca
      241 tccaaaaatg atgttaaaaa ttatcttata tgcatacact caatctgtat tttctggtcg
      301 tagaatagag aaattacttc atgacagtat tcgaatgatg tggttagctc aaaatcaaac
      361 accttcttat aaaactatta atcgttttag agtaaatcct aatactgatg cattaattga
      421 atctttattt atccagtttc atagtcaatg tttaaagcaa aatcttattg atcataattc
      481 aatttttatt gatggtacaa aagtagaagc taatgccaat aggtatacat ttgtgtggaa
      541 gaaaagtatt ccaaatcacg aatcgaaatt gaacgaaaat tcaaaaacac tatatcgtga
      601 cttagttgaa gaaaagctga tttctatcaa ataattaata gaaatcagct tttttacatt
      661 gcctaagaac ttaatgttcc agcctgagcg tgtcattaac ataataataa tacaatttta
      721 gcagtgtgta tctcttattt taaaatatta atcaatttat cgagaacatc atcatgttta
      781 ttagcttttt caactaataa ctcagtaaat ttattattcg tttctttatt aaattcacca
      841 gttacctcga gtttattcgc ttgttggaaa gctttaactt gattttctaa agcttgatca
      901 aattgcgttg tttcattatc aactttataa cctaaagctg acaaaccaat tttaatagtt
      961 ttaatatttt tatcatcgtc tccaacttta aatgttttcg tattaggaat gacatttaaa
     1021 gattgatatt taggtgtgtc aatagtaacg tctggtttaa tgcctttacc gtgaatataa
     1081 tgaccatctg gcgttaacca tttcatttca gtatatttta acaatgaacc atccttaaac
     1141 tctcttgtag tttgtacgac acctttgccg aatgtttttg acccataaac tttagcttta
     1201 ttatagtctt ttagcgcacc agtaaacact tcagaagcgc tagctgaacc ttcattcact
     1261 aagatggata tatccatgtc tttcgcttct tttaacgcat cattagaagt ttgaattgct
     1321 tcagtatctt tacctttttc tagtttaaca acagtttttc ctttatcgat aaaaatattt
     1381 gccattttaa cagcttcatc tagtagtcca cctggattat ttcttaaatc taaaacaatc
     1441 tttttcaaac catctttgtg agcttttaga actgcatctt tcaattcacc tgatgtatca
     1501 ttctggaatt tattaatagt aataactcca actttacctt ttttcttata ctcaacactt
     1561 ttaacatgaa ttttttcacg tttaatctta acgtcttttt cttcactacc tcgttgaaca
     1621 gttaaagtga cttcagtgtt ttctttacca cgaacatctt tgacaacttc atctaatgct
     1681 tttcctttaa ttgattttcc atttacttta gtaatgacat ctttaggacg aatgccagca
     1741 cgttctgctg gagatccctt cataggacta gtaaccataa tttgatcatt tttcttttgc
     1801 atttctgcac caatacctac aaaatcacct gaaacacctt cattaaagga tttcgtttgt
     1861 tcttttgtta aatattcaga ataaggatct tttaattctt tgaccatgcc atcaattgca
     1921 gctttactta acttgtcaga gtcctgtttt ttgtaataat cactatttaa gattttatac
     1981 acattttcaa ttttatttaa gtttgcttga tcagttttgt ttaaaccact tattttttga
     2041 tttataaaaa tatatgcaac aactgtgatg acagctgtta ttaggattgt accaattaat
     2101 attgatatga attgccaacg ttttaagtga acgcgtttcg atgaattagt ttcttggtct
     2161 tgattgcttg ttgcaatttc agcgcgttca tcatcggatg aagatgtgtg ttgcttatca
     2221 tccattgtat cacttcctta ttatgtatta tgtactttta aattctgtgt agttaatcat
     2281 atcaatttat gcaagatagc gcaccatatt tcactataat tgattaattt ttagtcacaa
     2341 cttagtttat tttaaattaa a
//
LOCUS       DBNENW010000235         2321 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.239, whole genome shotgun sequence.
ACCESSION   DBNENW010000235 DBNENW010000000
VERSION     DBNENW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.239"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(350..454)
                     /locus_tag="AC1LW8_002653"
     CDS             complement(350..454)
                     /locus_tag="AC1LW8_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774400.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            549..1862
                     /gene="sbi"
                     /locus_tag="AC1LW8_002654"
     CDS             549..1862
                     /gene="sbi"
                     /locus_tag="AC1LW8_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HIP4774401.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNVKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDKAVLTLLGDGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTITGLFNSFWK"
BASE COUNT          907 a          422 c          312 g          680 t
ORIGIN      
        1 ttttatgagc tagaaaggct tatgcattag tagcgaagct aactaatgcg ttcctttatt
       61 ttctgcgaat tttatgagct agaaaggctt atgcagttga cgttttacgt ccaactgcgt
      121 tccttcgtct tcttcaaatt tatttgttag aaaggtgccc aacccttcct gcttgttaac
      181 tttcgtctcg aaaatttcta tgttgggacc ctatgcatta gttgcgaagc tatctaatgt
      241 catgacctaa ttatatagaa gaaaaagtgc atcagtgaca aattaaatga gatttctact
      301 ctaccaaact ctcttcgaaa gacaattttc tcctctattt attagcaaac tattgcattt
      361 ctccatatag tacttcctta cttaaaatac gctgaatgtt ctgaattaaa cgctttttta
      421 catagttaac actagttaat ctattagtta acattagtta ataattagtt aatttccatt
      481 tgtattctca tgtgataaat tctaaaagca tacaataaat ttaatatgta aaaagaaagg
      541 gaatacacat gaaaaataaa tatatctcga agttgctagt tggggcagca acaattactt
      601 tagctacaat gatttcaaat ggggaagcaa aagcgagtga aaacacgcaa caaacttcaa
      661 ctaagcacca aacaactcaa aacaactacg taacagatca acaaaaagct ttttatcaag
      721 tattacatct aaaaggtatc acagaagaac aacgtaacca atacatcaaa acattacgcg
      781 aacacccaga acgtgcacaa gaagtattct ctgaatcact taaagacagc aagaacccag
      841 accgacgtgt tgcacaacaa aacgcttttt acaatgttct taaaaatgat aacttaactg
      901 aacaagaaaa aaataattac attgcacaaa ttaaagaaaa ccctgataga agccaacaag
      961 tttgggtaga atcagtacaa tcttctaaag ctaaagaacg tcaaaatatt gaaaatgcgg
     1021 ataaagcaat taaagatttc caagataaca aagcaccaca cgataaatca gcagcatatg
     1081 aagctaactc aaaattacct aaagatttac gtgataaaaa taaccgcttt gtagaaaaag
     1141 tttcaattga aaaagcaatc gttcgtcatg atgagcgtgt gaaatcagca aatgatgcaa
     1201 tctcaaaatt aaatgtaaaa gattcaattg aaaacagacg tttagcacaa cgtgaagtta
     1261 acaaagcacc tatggatgta aaagagcatt tacagaaaca attagacgca ttagtagctc
     1321 aaaaagatgc tgaaaagaaa gtggcgccaa aagttgaggc tcctcaaatt caatcaccac
     1381 aaattgaaaa acctaaagca gaatcaccaa aagttgaagt ccctcaaatc caatcaccaa
     1441 aagttgaggt tcctcaatct aaattattag gttactacca atcattaaaa gattcattta
     1501 actatggtta caagtattta acagatactt ataaaagcta taaagaaaaa tatgatacag
     1561 caaagtacta ctataatacg tactataaat acaaaggtgc gattgacaaa gctgtattaa
     1621 ctttacttgg cgatggttct aaatcttata tccaaccatt gaaagttgat gataaaaatg
     1681 gctatttagc taaatcatat gcacaagtaa gaaactatgt aactgagtca atcaatactg
     1741 gtaaagtatt atatactttc taccaaaacc caacattagt aaaaacagct attaaagctc
     1801 aagaaactgc atcatcaatc aaaaatacaa taactggatt atttaactca ttctggaaat
     1861 aatcaatcaa aaatatcttc tctagtttta catcattttt taaataattt tcgtaataaa
     1921 ccgtgattaa aaagaaccgc tgattctcaa tcgaatctac ggttcttttt tcattttcca
     1981 tcaattaaat gtttcttcgc tatttgtcag cccacttttt tacctgcaac ttgttaaata
     2041 atccttacat cgttaacgaa tagttcatca tttagttgaa tcagctcaac tttattaact
     2101 tcaaattttc acaaactatt gcgcaatcca ttcctcttcc actacaagca ccataattaa
     2161 acaacaattc aatagaataa gacttgcaaa acatagttat gtagctatat aaacgcctgc
     2221 gaccaataaa tcttttaaac ataacataat gcaaaaacat catttaacaa tgctaaaaat
     2281 gtctcttcaa tacatgttga tagtaattaa cttttaacga a
//
LOCUS       DBNENW010000236         2312 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.240, whole genome shotgun sequence.
ACCESSION   DBNENW010000236 DBNENW010000000
VERSION     DBNENW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.240"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..717)
                     /locus_tag="AC1LW8_002655"
     CDS             complement(<1..717)
                     /locus_tag="AC1LW8_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP4774402.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPI"
     gene            complement(1078..1578)
                     /locus_tag="AC1LW8_002656"
     CDS             complement(1078..1578)
                     /locus_tag="AC1LW8_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4774403.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(1815..2237)
                     /locus_tag="AC1LW8_002657"
     CDS             complement(1815..2237)
                     /locus_tag="AC1LW8_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIP4774404.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
BASE COUNT          771 a          366 c          375 g          800 t
ORIGIN      
        1 gattggacca cgagaggcat tgattaaata cgccgtagat ttcatcattt taaattgttc
       61 tgtatcaatt aaatgatgca ttttaggatt ataagcagcg ttgatagtga taaaatctgc
      121 attctttaat agtgtatcta aatctacata ttttgcaccg atttctcgtt ctttttcttc
      181 tttgcgatta ggtcctgtgt atagtacatc catgtcaaat gctcttgcac gacgcgctac
      241 tgcactacca atttcaccta aaccgataat gccgattgtt ttcccagata cttctctacc
      301 tctgaaaaat aaaggtgccc atccatcaaa tccagttgta cgtgataatt ggtccccttc
      361 aacaatacga cgcgctactg caagtactaa tccaattgtt aaatcggcag tcgcgtttgt
      421 tgatgcttta ggtgtgtttg taacatctat acttttttct cgggcatact cgatatcaat
      481 attattaaaa ccagcgccat agttggcaat gatttttaag tctttaccag catcgataac
      541 atctttatca acgtttgtag ataataaact aattaaggca gtcgcgtttt taacaccttt
      601 aattaaagtg tctttatcga ctaatccttt accttcatac atttcaactt caaaatgttc
      661 ttgtaaaagt tttaaaccta cttctggtat tgcaccagca acataaactt tttccataaa
      721 agatcactcc ttttatctta gtatagtaga agattagaca gtatacaact atgtcatgat
      781 gtcttgtgta tcaatgatgt aagcgcgtac ttttgatgga ggtgatataa cttaggcact
      841 gtagaactat gaatattgta atgtggaaaa actggatcaa ttaaattaga taacgtagtt
      901 ttaaagttaa tagtattaga aaaaattaat attttgaata tgggaggaaa tataaataag
      961 taggtggcaa cgaaaaatag caaaaaagag cttctcctat aaaggaaagc tcaaagtttt
     1021 ttgatgacat atgtactaga attaagtttc aagacaatat gtatcatcgt gtttatatta
     1081 aatatggatg tagttgtagt tacctgcttc acttgcagaa atagttctag aacttactga
     1141 gaaaggtccg ccactataat tcatttctga aattgtaact gaaccatcac tgtttacact
     1201 ttctacatat gcaacgtgac caaatggtcc ttcagaagat tgtaggatag cgcctttaga
     1261 aggtgtatga tttactgtga atccagcacc ttgtgcagca gcagcccaat tattagcatt
     1321 tccccaagta gaaccgattt ctccgcctac tttatcatat acataccaag tacattgtcc
     1381 tgcagtgtat aagttaccag aatgtgaaat tgatgatgta gttgtcgtag ttgttgtagt
     1441 cgttgtagtt tgagtcgtgt tgtagttata gttgttgtaa tttgtataat tttcagcagc
     1501 atctgcatga tgtgcttgac ctactaatgc tgtgccgatt cctgctgtta acgtagttgc
     1561 tgttactaat tttttcatga ataaagtcct ccaaagttct atatcttttt ttataaataa
     1621 aacgtagcga ctgttttatt ctcacatctc gaattgatga caatagttac tttaacaaaa
     1681 ttaatgcttc ttgtggggaa tgttattgat ttgtaaaaga ataaaaaaac tttgactaat
     1741 tttgtaataa aaattagtca aagttacaat gagattaaca gataattaat aggaaatatt
     1801 tatttgtaat atgtttaaat aaatcgaatt gttaaaggta ttacatattc ttgaccatta
     1861 taatatttga cacacgcaat aattgtgaat acaaaagata atattgagaa agcgaatatg
     1921 gataaaatac cgataaatgt aatgatgaaa cctataatga taatgaaact aatatcggta
     1981 gcaattagga aaacgcctat taaagtgata acgactaaaa cgatagacca aataatataa
     2041 gaaatcgtat agttaagata attttttcca gcacgatcaa ctagtttcga ttcatctttt
     2101 ttcaataacc atataatcag tggaccaata atagatgtga ataaacttaa taaatagata
     2161 agcatcgcca taatgttctc atcattggat ttgcgattcg gttgatgatt tgttacgtcg
     2221 ttcatttcag ttgtcatatt agacactcct ttgaaaattg taatattatc tttaactata
     2281 acaaaatata atcaaaaata aacatgttta tt
//
LOCUS       DBNENW010000237         2295 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNENW010000237 DBNENW010000000
VERSION     DBNENW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.241"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1446
                     /locus_tag="AC1LW8_002658"
     CDS             <1..1446
                     /locus_tag="AC1LW8_002658"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP4774405.1"
                     /translation="VNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKK
                     QGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDI
                     TTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEK
                     GYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGS
                     FPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRL
                     DILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKK
                     DNSVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEG
                     QGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNIKDMADKILQLVNNDVL
                     AAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1535..1891)
                     /locus_tag="AC1LW8_002659"
     CDS             complement(1535..1891)
                     /locus_tag="AC1LW8_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIP4774406.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          927 a          264 c          371 g          733 t
ORIGIN      
        1 gtcaataaag gtggtatgac ctcttcgatg ttcaatagaa gtaaagagtt ttatgatgcg
       61 gacatacctg ctgatattgt tactttcgat tacaaaggaa actatgatga aattattaaa
      121 gctttgaaaa aacaaggtaa aatggatcga agaacgaaaa tgtataatgt atttgaatat
      181 tttaaacaaa tttcaaataa taaacatttt aagtctaata aattgttata taaacatatt
      241 tcagaaagac taaaaaatac gattgaaatt gaagagagta aaggtatttc aagatatttt
      301 gatataacga ctggtacata tattgcctac attagaaaaa gtaaatctga aaaagtgatt
      361 gatttcttta aagataataa acgaattgaa cggtttagtt ttatagataa taaagtgcat
      421 atgaaggaaa catttaatgt agataataaa gtttgttatc aagtatttta tgatgaaaag
      481 ggatacccat atatttcaag gaatattaat gctaataatg gtgctgtagg taaaacttat
      541 gtgttagtta ataaaaaaga atttaaaaac aatttagcac tgtgtgttta ctatttagaa
      601 aaactaataa aagattctaa agatagtatt atgatttgtg atggaccagg gagttttcca
      661 aaaatgttta atacaaatca taaaaatgct cagaaatatg gcgttattca tgttaatcat
      721 catgaaaatt tcgatgatac gggtgcattt aaaaaaagtg agaaatatat tattgagaat
      781 gcgaataaaa ttaacggtgt aattgtatta acagaggcac aaagattaga tattcttaat
      841 caatttgatg tagaaaatat tttcactatt agcaattttg ttaagataca taatgctcca
      901 aaacattttc aaactgaaaa aatcgtaggt catatttcta gaatggtacc aacgaagcga
      961 attgatttgc ttattgaagt ggctgagtta gtcgtaaaaa aagataattc tgttaaattt
     1021 catatatatg gagaaggatc tgtcaaagat aaaatagcta aaatgattga agataaaaat
     1081 ttagaaagaa atgtttttct taaaggatat acaacaactc cacaaaaatg cttggaagat
     1141 tttaaattag tcgtttctac atctcaatat gaaggtcaag ggttaagtat gatagaagca
     1201 atgatttcta aaaggcctgt tgttgccttt gacatcaaat acggaccaag tgattttata
     1261 gaagataata aaaatggtta tttaatagaa aaccataata ttaaggacat ggctgataaa
     1321 atacttcagc ttgttaataa tgatgtatta gcagcggagt ttggttcgaa agcgagagaa
     1381 aacattatag aaaaatattc aacggaatca atattagaaa aatggttaaa tcttttcaat
     1441 agctaaatga aaaaatccta ggtattcatg agtacctagg atttttacgc atgatatata
     1501 caaaaaaaga gaggatagtc ctctcaaata atagttaata aaattgtagc catttcttcc
     1561 agctgtaact actaatacaa gctaagttca ttacgatatg tatgatttca aattcataac
     1621 cacttacata tcctcttgta agtttaagtg tgaagattga gcctaacata acacaaatat
     1681 aaaaaacatt aacataaggc gttaaaaagc cgataataag tgctaaacct ccaacaactt
     1741 caccaattga catcaatact gcgatggtag gtggtaaacc taaagagcct aagaagtgaa
     1801 ttgttccatc taaatcaaca aatttcaaag taccatgcac taagaatgat gttgctaaca
     1861 tccatctaat taaaagcata cctaatttca tcatgtcacc tccaacatac gtatttttaa
     1921 aacatagtcc aaattatatt tgtaaagatg tggaaagtaa agattattta taaaaataat
     1981 tattaaatat ttattaaatg ttctattgta ttgaacaatt gttgtagaaa gacgtttaag
     2041 tcatcagtta cattttgtat ttataaataa agattagcac agcatagtac aaagtttaga
     2101 ggtaaatgaa ttaattattg catcgctatt tatgaatcgt aaacactatt atgaaattgc
     2161 aagagtagta tggctaatta gtatgatggt ttgagataaa tgcgtgttat taaccagtga
     2221 gtcatcttca aaattagagg ccatttgctg tttataataa gatgatttga taatcaacta
     2281 tgataagaaa aaata
//
LOCUS       DBNENW010000238         2289 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNENW010000238 DBNENW010000000
VERSION     DBNENW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.242"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(316..510)
                     /locus_tag="AC1LW8_002660"
     CDS             complement(316..510)
                     /locus_tag="AC1LW8_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774407.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGVVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            complement(521..1168)
                     /locus_tag="AC1LW8_002661"
                     /pseudo
     CDS             complement(521..1168)
                     /locus_tag="AC1LW8_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(1239..1361)
                     /locus_tag="AC1LW8_002662"
     CDS             complement(1239..1361)
                     /locus_tag="AC1LW8_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774408.1"
                     /translation="MSIQQLDGYISIEDFFKHMDELSKKEELEKEINQSKSKYQ"
     gene            complement(1374..1643)
                     /locus_tag="AC1LW8_002663"
     CDS             complement(1374..1643)
                     /locus_tag="AC1LW8_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774409.1"
                     /translation="MLHNHPGQSGFSLNGLKMFIGNKSIQTTTIVTNYSVVKHISKTS
                     LYNQSQVYKIMKDIKQSITMRNNEAIVDNILKQLYNKTYIKRKDK"
     gene            complement(1763..1975)
                     /locus_tag="AC1LW8_002664"
     CDS             complement(1763..1975)
                     /locus_tag="AC1LW8_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774410.1"
                     /translation="MEKNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKDAVQEAEKLAKMK"
     gene            complement(1942..2070)
                     /locus_tag="AC1LW8_002665"
     CDS             complement(1942..2070)
                     /locus_tag="AC1LW8_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774411.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYVSWRKMKVILLT"
     gene            complement(2135..2257)
                     /locus_tag="AC1LW8_002666"
     CDS             complement(2135..2257)
                     /locus_tag="AC1LW8_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774412.1"
                     /translation="MYFAQLDGEITNKQSQELLDKEYKKAIELENKNKEQKLEK"
BASE COUNT          672 a          370 c          283 g          964 t
ORIGIN      
        1 agttatttta tgaaagtgat gtaatatcat ttgaattact aaatctattt tggtaggaat
       61 atcgatgttt agtttaaatg gaatgcacta tttaagtttt taatatggag atgtttgtga
      121 catttcgatg attaagattt ttataggtgt gcatctttac aaataaactt tgtgtttaaa
      181 attgaactta ggaaatcgat aggtttagat gaagattttg ttgaaggtat gtgtcttgta
      241 tccttagttg ttataaaagc gcaaaaaatg gcatcacttt atctttatct gtgtagaaag
      301 gatgctattt agaaattatt ttaataatct aatacttttt atctcagttt catcaaacga
      361 ataaacagca aaatcagtct gtatatctac aacaagattt cctgtttcac tctcaaattc
      421 attttcatag tcagttacaa ccccttcgta ttcttcatta ttcaaaagcg tgataataac
      481 atctttacga tatgcatctt caattctcaa tacttttcac ctacttatca atataaggta
      541 ctatatgagc acctgtcttc gaataatgca cttttccttt tctggtttca atatacacat
      601 tatcgatatg tacttttcca ataatttgat taaaattaat aatctctttc atatcaaata
      661 gctcatcact taatattaga ttgcctgttg acattttttc tcttaacaat tcattcaata
      721 aatctataga aagtattgta tagctaggca atttcttatt atttaaaatg gctctttttt
      781 tattttcatt atatagatga tgacctaaca tatgtccatt ttgtttttca caatttattt
      841 ctattttcat tttacaactt tcaatcattt ctttcatctg gtttttagct ttttcttgta
      901 atttagcttc ttttacttct acctgatatt taccttcacg ctctttaaag aacttgtctc
      961 gccaactgcc aacatgtggc actgtggtac ttctacacta aggatgcata ggtggcgcat
     1021 tcacacctgg tatcatatct ttaactttaa atatttttcc gttgagtgaa tgacataatt
     1081 tagatgtttt actatctatt ttggcaacat atttatattc gccatcttca ccaagttctt
     1141 ttaaatatgt taacttttgt gattctgcat tttcagtaaa tagttaaaaa agcgtataaa
     1201 aatagcacca ccttctcttt atctgtgtag aaaggatgct attgatattt agactttgat
     1261 tgattaattt ctttttctaa ttcttctttc ttcgataatt catccatatg cttaaaaaaa
     1321 tcttctatag aaatgtaacc atctaattgt tgaattgaca taatgtatta ctcctatttg
     1381 tcctttcgtt ttatatatgt tttattataa agttgcttta gaatattatc aactatcgct
     1441 tcattatttc tcatagttat agattgtttt atatctttca taattttata aacttgagac
     1501 tgattatata atgaagtttt acttatatgt tttactacac tatagttagt aacaatagtc
     1561 gttgtttgga tagatttatt tccaataaac atctttaaac cattcaatga aaatcctgat
     1621 tgtccaggat ggttatgtaa cataacaatt gaattcggtt tactgttaaa taataattcg
     1681 gttgcttgtt accctggcac aaaagataca ctatcttgat cgccatatac ttttgtaact
     1741 ttagcacctt ttaataaata agctacttca ttttagctaa cttctcagct tcttgaaccg
     1801 catctttaat acgttcatct gattttttgc gttctctagc tatacgctct ttaatcaatt
     1861 gtgatacttc ttcttgagaa aatgttttct cattctgttg tgattgttca tcactgttgt
     1921 ctagttgctc ttcgttctga gttacgtcag taatattact ttcatttttc tccatgagac
     1981 atacctccgt ttatagtctg tcgactgata ttccatactt gcttttaaaa tgtcatcagc
     2041 acgttttgga caataaaaaa taaccttcac attggaaggt taaaaaagtg tataaaaata
     2101 gcaccacttt ctctttatct gtgtagaaag gatgctattt ttctaatttt tgttctttat
     2161 tcttattttc taattctatt gctttcttgt attctttatc caataattct tgtgactgtt
     2221 tattagttat ttcaccatct aactgtgcaa aatacatttt aatcacctaa ctttatatat
     2281 tatgttacc
//
LOCUS       DBNENW010000239         2248 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNENW010000239 DBNENW010000000
VERSION     DBNENW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.243"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..859
                     /locus_tag="AC1LW8_002667"
     CDS             <1..859
                     /locus_tag="AC1LW8_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIP4774413.1"
                     /translation="TLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLI
                     NGCVCCDLKQELVYELKAIALKGDVNHVIIETTGIAHPLELLVACQDPQIVNFFEKPI
                     IYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDRLERIDKQLGMIC
                     ASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIM
                     KLPESVLRLKGYVSFGDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIR
                     NQLDMLQFT"
     gene            962..1870
                     /locus_tag="AC1LW8_002668"
     CDS             962..1870
                     /locus_tag="AC1LW8_002668"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP4774414.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRSSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            1905..2192
                     /locus_tag="AC1LW8_002669"
     CDS             1905..2192
                     /locus_tag="AC1LW8_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774415.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
BASE COUNT          770 a          310 c          396 g          772 t
ORIGIN      
        1 cacgttactg acacattata ttagtgaatt attaaaaaat gatgagaaaa ttaaaatcat
       61 catgaatgaa ttcggtactt ttgatattga tagcaatagt atttcaaatg aaattgaagt
      121 ccattcattg attaatggtt gtgtttgttg cgatcttaaa caagaacttg tctatgaact
      181 aaaagccatt gctttaaaag gggacgttaa tcatgtcatc atagaaacga caggcattgc
      241 gcatcctttg gaattactag ttgcatgtca agatccgcaa atcgttaatt tctttgaaaa
      301 gccgattatt tatggtgtat tagatgcgac tcgattttta gaacgtcatc aatataccga
      361 aaatacagtt tcgctgatgg aagatcagtt gaaactaagt gacatgatta ttattaataa
      421 aattgatctt ataactgatg accgtcttga gagaattgat aagcaattag gtatgatttg
      481 tgcaagtatt ccaacatata aaacaaccta tggaaaagtt tcgttggaag aattggactt
      541 aactgttaaa gacagagaga tatcgtctca tcatcaccat catcatggga ttaaaagtat
      601 gacttacacg tttacaggtc cgattgatcg tcaattgttt tatcaattta taatgaaatt
      661 accggaatct gttctacgtt tgaaaggtta tgtgtcattt ggagatcaac caaatgcaat
      721 ttatgaattt caatatgcat atggtttacc agactatgga ataattggca tgcaattacc
      781 attaacgatt gttattattg gtgaaacttt agatacaaat cacatacgta atcaattgga
      841 tatgctacaa tttacgtaaa tcagaaaatg tgttgtttca atttaacgga aatagcacat
      901 taatttctta atacttttat tgtgaatatt ttgtgacatt gagggatgga gtggatgaga
      961 tatggaacaa ataatgatta atcactatgt tcatttttct aggcttgtac aaggtttttg
     1021 gcgtgcaaat gaatggaaga tgactgcgaa agagttaaat tattttataa atgaattagt
     1081 tgaacgtgga attacaacga tggatcatgc tgatatttat ggggattatc aatgtgaatc
     1141 actgtttggt aatgctttgg atttatcacc cgaattaaga aataaaattc aaattgttac
     1201 gaaatgtggt atcattttgc cttctaagca atttgatttt acaaatggac atcgttatga
     1261 tttgagtagt aagcacatcg tgaaatctgt tgaacagtca ttaatcaatt tgaatgtaga
     1321 ttatttagat agtctactca ttcatcgttc ttcaccattg atggatccag aacaagttgc
     1381 tgatgcatta actaaacttg ttaaacaagg taagttgaag tcattcgggg tgtcgaattt
     1441 taatcattca caataccaat tgttaaatca atatattatg aaagaaagac tacatattag
     1501 catcaatcaa ttagaattat cgccatatca cgttgatagt ttacaagatg gaacaatgga
     1561 ttcaatgtat caaaaccatg ttcaaattat ggcttggagt ccttttgcag gcggtaaaat
     1621 tttcgacaag gaagatatta aagcgcaacg tattatgaaa gttgttcaat caatagctga
     1681 caaatatggt gtgagtgaca cagctgtgat gatagcatgg ttagtaaaaa taccgcatcg
     1741 tatcatgccg atacttggaa caagtcagtt aaagcgtatt gatcaagcaa tcgaagggct
     1801 acaacttaat ttagatgatc agtcgtggtt tgacatttac accgctatta tcggacaaga
     1861 tattccgtaa acttatttac ttttaaatca taaaggagca tatcatgaca aacgaagata
     1921 aacgtttcga acaattaaga tttgaacgca aatttatagt tattccgtat ttaatttatg
     1981 cagtcattgt attactatta aatattttct attctgattt gaaaataaca atgacattat
     2041 tcggactttt ctttgcgtat aatgtagtca ttttgttcat agcatttatt aaacattata
     2101 aacgcacatt gttactaagt cttatattaa cagtgcttag tggcgcggca ttctttggaa
     2161 ttatttatgt ttatggcatt aatcattttt aaatattaaa aagagactat ctacaataat
     2221 ctcatttgca ttttatctaa ctaagttt
//
LOCUS       DBNENW010000240         2156 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNENW010000240 DBNENW010000000
VERSION     DBNENW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.244"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            49..1083
                     /locus_tag="AC1LW8_002670"
     CDS             49..1083
                     /locus_tag="AC1LW8_002670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP4774416.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            1119..1520
                     /locus_tag="AC1LW8_002671"
     CDS             1119..1520
                     /locus_tag="AC1LW8_002671"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774417.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDWLIGMGRVIGDGGTAFQIVDIAVSKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKF"
     gene            complement(1796..>2156)
                     /locus_tag="AC1LW8_002672"
     CDS             complement(1796..>2156)
                     /locus_tag="AC1LW8_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774418.1"
                     /translation="VNYIPRNFFIILFPVAFMTISITNISLASIQQKVIPEHLLARLT
                     TIITSISAISLPMGSFIGGILIKNLGIDYTLVIGGALFIIASIIFFISTPYRSLPKIE
                     KIEHYHIFGIQKIGNNH"
BASE COUNT          764 a          266 c          441 g          685 t
ORIGIN      
        1 agcgcgttaa tgaaagaaat gaatatagag agtcatagga ggcacataat gaaagacgtt
       61 acaatcattg gtggcggtcc gtctggtttg tatgcaagct tttatgctgg attgcgagat
      121 atgtctgtaa gattgataga tgttcaatct gaattagggg gtaagatgag aatttaccct
      181 gaaaaaatta tttgggatat tggcggcatt gcgccaaaac cttgtcatga aattttaaaa
      241 gatacaatta agcaaggatt atattttaaa ccggaagttc atttgaatga acgtgtggta
      301 gatattagga aaaaggcaga acgtcatttc gaagttgaaa cagaagcggg agaaatatat
      361 acatcgaaag cagttatcat tgcaattggt gcgggtatta ttaatccaaa acaattagat
      421 gttaaaggtg tagagagata tcaattaact aatttacatt atgttgtaca aagttacaga
      481 cgtttcaaag ataaagatgt attaatatca ggtggaggaa atacagcatt agattgggcg
      541 catgatattg ccaagattgc taaaagcgtg acagttgttt atcgaaaaga agatgtaagt
      601 ggtcatgaag cgatgaaaac gttggtgaca gatttaaatg tgaaactatg cccaaaaaca
      661 cgtattaaat acttggtcgg caatgatgat gaaacgcata taagtgaagt tgttttggaa
      721 catgttgaaa gtggcgatac gcatacagtt aaatttgacg atgtcattat tagtcatggg
      781 tttgatcgtt gtaatacatt attgagtgaa acatcttcaa agctagatat gcatgatgat
      841 tgtcgtgtta aaggatttgg taatacgaca actagtatac ctggtatcta tgcgtgtgga
      901 gatattgttt atcatgatgc aaaatcacat ctaattgcaa gtgcatttag tgatggtgct
      961 aatgcagcga accttgctaa aacatatatc caaccagatg cgaatgcaga gggttatgtt
     1021 tcaagtcatc atgaagtgtt taaagaagcg aataagacta ttgtaaataa acatttatac
     1081 taggttgata tattgtgtga tgaaaagtgg ggtaaataat ggttaaagtg acatatgata
     1141 ttccgacttg cgaggattat tgcgcattaa ggattaacgc aggtatgagt ccaaagacgc
     1201 gcgaagcagc tgaaaaagga ttacctaatg ccttatttac agtaaccttg tatgataaag
     1261 attggttaat tggtatgggt agagtaattg gcgatggcgg aactgctttt caaattgttg
     1321 atattgctgt ttcgaaaagt tatcaaggtc aaggttacgg cagtcaaatt atggaacata
     1381 ttatgaagta tattaaaaat gtatctgtcg aaagtgcata cgttagtctg atcgcagact
     1441 acccggcgga taaattatat acaaaatttg gatttatacc tacggaacca gattcaggtg
     1501 gaatgtatat taagttttaa aaataaaaag acatctcgca aaacgggatg tctttttgat
     1561 gggaaaatat ttcccgattt agatagatat accaatgatt agacgaagaa aagcatttaa
     1621 ttaactagtt ttatctaata aatcattagt gattttttgt ttaaaatttt tgtattcagt
     1681 aaacaattct tcttgagttt tgacattttg tgagtcagtt tcatagatca attctaatac
     1741 taaatctatt ttgaaaacct atttaagtat attgaactaa taaaaatgta taattttaat
     1801 gattgtttcc aattttctga attccgaata tatgataatg ctctattttt tcaattttag
     1861 gcaatgatct gtaaggtgta gatataaaga aaatgatact tgcaataata aataatgctc
     1921 caccaattac aagagtataa tcaataccaa gattttttat taggattccg ccaataaaac
     1981 tacccatcgg taatgaaatt gctgaaatac ttgttatgat tgtagttaat ctagcaagca
     2041 aatgttctgg aataaccttt tgttgaatag aagctaagga tatattggta atactaattg
     2101 tcataaaagc tactggaaac aaaattataa aaaaatttcg cggaatatag ttaact
//
LOCUS       DBNENW010000241         2132 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.245, whole genome shotgun sequence.
ACCESSION   DBNENW010000241 DBNENW010000000
VERSION     DBNENW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.245"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(253..675)
                     /locus_tag="AC1LW8_002673"
     CDS             complement(253..675)
                     /locus_tag="AC1LW8_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIP4774419.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVSPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            823..1539
                     /gene="aroD"
                     /locus_tag="AC1LW8_002674"
     CDS             823..1539
                     /gene="aroD"
                     /locus_tag="AC1LW8_002674"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIP4774420.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            1622..>2132
                     /locus_tag="AC1LW8_002675"
     CDS             1622..>2132
                     /locus_tag="AC1LW8_002675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIP4774421.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLSGFLYLTD
                     LEEDMPKAPR"
BASE COUNT          741 a          313 c          388 g          690 t
ORIGIN      
        1 taagttcagg aaattatgga attggttatt ggtcgacact ggacttttat ttgtagacgg
       61 tatgcgaaga agtcaagcca gtattggaac cggtcacatt tatgatgact aagggacaat
      121 cattttcagt taaatattgc cagaaatttc agtcacgaat agaaaaaata accaaccaaa
      181 aatttttggt tggcaaattt gaattttatg tgaattttat taataacgtt gaataatctc
      241 tttaagttaa tgctaatcta caacatttac atttaaatcg acattaatat ttccttgagt
      301 cgcttttgaa tatggacaaa attcatgagc catttgtaaa tatttttcag cttcttcttg
      361 agatataaca tttttaattg tcgcatcaat tgaaacactt aatttaggac tttctgagtc
      421 tgaatcatct tctagtctca ctgttagtgt tacttctgga tgagcatcac gcactttgtt
      481 ttgctttaaa attaggtcga aagcaccgtt gaagcaagat gcataacctg ctgcaaataa
      541 ttgttcgggg ttagtagctt taccatctgc ttgagcaggc gaaacgatat caatatctaa
      601 cgctcgatca tcagtataaa catgtccttt acgtccgcca acattcgtcg ctttagtttc
      661 ataatgtatt gccattctta taacctccta atttaaaata catttgtgct ttacccattc
      721 gaaaatacct taatcatttt catttatagc attgtttgat tgaaggataa aaagttgttg
      781 tttacaataa aaataatgag tatctgaaat gagggattta ctatgacaca tgtggaagta
      841 gtagcgacta tcgcgccaca attatctatc gaagaaactt taattcaaaa aattaatcat
      901 cgtattgatg caatagacgt attagaatta cgaattgatc aaattgaaaa tgtcacagtt
      961 gatcaagtgg cagaaatgat tacaaagctg aaggttatgc aagattcatt caaattatta
     1021 gttacgtatc gtacaaagtt acaaggtggc tatgggcaat ttataaatga cttgtatctt
     1081 aatttaatat cagacttagc aaatatcaat ggcatagata tgattgatat agaatggcaa
     1141 gcagatattg acattgaaaa acatcaacga atcattacac acttgcaaca gtataataaa
     1201 gaggtggtta tatcacatca taatttcgaa agtacgcctc cattagatga attgcaattt
     1261 atatttttta aaatgcaaaa attcaaccca gaatacgtta aattagcagt aatgccacat
     1321 aataaaaatg atgtgttaaa tttattgcag gcaatgtcta cattttcaga tactatggac
     1381 tgcaaagttg ttggtatttc aatgtctaaa cttggactaa taagtagaac ggctcaaggc
     1441 gtttttggtg gtgcattgac ttatggttgt atcggagaac cacaagctcc aggacagatt
     1501 gatgttactg atttaaaagc acaagtgact ttatactaat aaaatgaatt aggttgcttt
     1561 tgtgtaagtg ctgtcttata attgagaagt attatcattg ttcgtggagg agcgatttta
     1621 gatggaatta caacaagcaa tagctaatag aagaagtgtg aaaaaattta aaagagatat
     1681 gcacatagat gacgcattgc tatatcaagc aattgagaaa gctgctgatg ctccaaatca
     1741 cggaatgagg gaaccatgga gagttgtgca tgttccgaaa gacagattag gagatatgag
     1801 taaggatatt tctaaatttg catttcctaa tgaattagat aagcaacaat gtcattatga
     1861 tgcagttacg aaactaggtg gcatgttatt gcttatttta aaaacagatc caagacaacg
     1921 tcaaaatgat gaaaactact ttgcatttgg tgcatatgca caaaatctta tgttgttact
     1981 ttatgaagcg ggaataggta catgttggaa atcgccatta tatatctatg atcctaaagt
     2041 aagaaaaaca cttggtataa agaaagatga agttctttct ggattcttat atttaacgga
     2101 tttagaagaa gatatgccta aagcaccacg ta
//
LOCUS       DBNENW010000242         2130 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNENW010000242 DBNENW010000000
VERSION     DBNENW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.246"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..289
                     /gene="scb"
                     /locus_tag="AC1LW8_002676"
     CDS             <1..289
                     /gene="scb"
                     /locus_tag="AC1LW8_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HIP4774422.1"
                     /translation="ATIEGNKADASSLDKYLTESQFHDKRIAEELRTLLNKSNVYALA
                     AGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVALEKIYKEIDEIINR"
     gene            complement(998..1183)
                     /locus_tag="AC1LW8_002677"
     CDS             complement(998..1183)
                     /locus_tag="AC1LW8_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774423.1"
                     /translation="MNKHINKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1802..2035)
                     /locus_tag="AC1LW8_002678"
     CDS             complement(1802..2035)
                     /locus_tag="AC1LW8_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774424.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          799 a          303 c          305 g          723 t
ORIGIN      
        1 agcaactata gaagggaata aagcagatgc aagtagtctg gacaaatatt taactgaaag
       61 tcagtttcat gataaacgca tagcagaaga attaagaact ttacttaaca aatcgaatgt
      121 atatgcatta gctgcaggaa gcttaaatcc atattataaa cgtacgatta tgatgaatga
      181 atatagagct aaagcggcac ttaagaaaaa tgatttcgta tcaatggctg atgctaaagt
      241 tgcattagaa aaaatataca aagaaattga tgaaattata aatagataat aaataaaaca
      301 ggttgagaca aaaaatggtc ttaacctgtt ttcaatttgc atatgtgata aattctatat
      361 caaaatgctt atgtataatg aatgacattt aaaagtaggg gagacaaata taaatacaat
      421 agttcctagg attactctca aaataactat atcaattatt tactttgctc tcctattttt
      481 taaaatatgt acatgtttaa acaatcaaaa gtgtacaata ttaaattatc atttccagtt
      541 ctagtgctat attggtagta gttgactaaa tgaaaataag cttataacaa gttttttcaa
      601 tactcgtggg gccacaacag agagaaatag gatcaccaat tccaacagac aatgcaagtt
      661 ggggtggggc ccaacacaga agctggcgaa aagtcagcat acaaaaatgt gcaagttggc
      721 ggggccacaa cagagagaaa taggatcacc aattccaaca aacaatgcaa gttggcgggg
      781 ccccaacata gagaaattgg gtcaccaatt tcaacagaca atgcaagttg gggtaggaca
      841 tcgataaaaa aatacttttt ctttagaaat tagtatttct tatgcatgag ttttactcat
      901 gtattcattt tttaagtaga cattagctac agctaatgat aaagaaccac tacataataa
      961 atcattagtg gttctttatc atttctatct cactctttta ctggaagaaa aagtttacgt
     1021 ttgtagaaca tgccacaata ccaaaaataa ttaagaaaaa taagacgata agcatgatga
     1081 cacttttcaa acaacctcta tcagtttctt ccgattttct ttgttgaacc tttttataat
     1141 cttcaagtag ttttgtcgct tttttattta tatgtttatt catgatgttg actccttata
     1201 atatatgttt aattcattaa aatagttgaa aacatgactt gaaataaaga tataaagcta
     1261 aagtaaggat gttcaattgt ttcaaatttt atgaaagtat aacgttaaat atgttaattt
     1321 gatgatattt gctttttatt ttccaaatgg aatttcctta aactgatgca ttaaaatatt
     1381 aatgaagcac tagaatacat aaatgaatag taatggtgca cagtatagaa taattaaggc
     1441 tatattaagt ataaatatcg ttaactgtaa gctatcttta gttttaatat aaactattag
     1501 gataatcgac gtaagaagaa tcatatatat taatgatgaa gaagtccata caaaatccgc
     1561 atcatttgtt gttaataatg ggactataat taatccgaaa ttaatcatgc atgctatata
     1621 tactataatg ttatacacaa tgttaatttt tgttcaccac cttatacttc tattttaaaa
     1681 acttctttat aatgatatat tgtttaatgt tgaaataatt agattatcta attttcattt
     1741 gctttacatg taaaaggcta tatatagtat gctctttatg attctaaatg ctttttaata
     1801 tttaatgctc atcaacattt ggattttgaa tattcaattc aaaaacttta ttagctacgt
     1861 caattgtaaa atcagaacca tagttgacat gagctacttt taattttcca tctaaataat
     1921 agattgcgat tgcaacatcg taaaattcgt caatgacaaa taaactcttt tcatttgtta
     1981 caacctcatg ctctcctgag tatacaacgt taatttccca atcattaaaa accatttgtt
     2041 aacctccttg aacatttaaa ttgattcaac ttaagtttaa cttattcata caacttcgta
     2101 caatatctag atgaacatta attgtatttc
//
LOCUS       DBNENW010000243         2122 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.247, whole genome shotgun sequence.
ACCESSION   DBNENW010000243 DBNENW010000000
VERSION     DBNENW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.247"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            25..885
                     /locus_tag="AC1LW8_002679"
     CDS             25..885
                     /locus_tag="AC1LW8_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4774425.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(1240..1773)
                     /locus_tag="AC1LW8_002680"
     CDS             complement(1240..1773)
                     /locus_tag="AC1LW8_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HIP4774426.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            1864..>2122
                     /locus_tag="AC1LW8_002681"
     CDS             1864..>2122
                     /locus_tag="AC1LW8_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP4774427.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMK"
BASE COUNT          833 a          256 c          313 g          720 t
ORIGIN      
        1 gggaacccta aaggaggtac tactttgaac ttaaacattt taaaatcatt tttagtaact
       61 agtgaaacaa agaaccttac taaagcatca gagttactta actattcaca gtcaactgta
      121 tctacacata ttgaaaaatt agaaaggcaa ttagatgtta aattatttta tagaaaaaaa
      181 tatggtatgg aactaacgga agaaggctta gcatacgtta aatacgctaa agtgatttta
      241 gatagtaata gcgaatacga gagagaaata aaaggacttt acaataagaa ggtaaatata
      301 agtattaaca tgcaagaaag tcagtatttg tatcgctact ataataagat tagtgaatgg
      361 ttagctgaac acccatatgt aaacttgaag tttaaatccg cacattctaa tttctatatt
      421 aaagaagaaa ttgctaattt caaatcggat attagcctta tcacagacga aaagattatt
      481 aatagtaact taactgctat tcctataact gaagaacgtt taatatttgt tactaacaaa
      541 gagatgaaag atttcaaatt aaacgatatt caaaaccata cattacttgt tactgaaaaa
      601 gggtgtagtt acagagaaca gttagaaatg attttatata aatatagctt ttcaacaaaa
      661 caaatgattg aatttctagg aattgagtca ttaaaaaaac acttgaaaaa ttcaggtggg
      721 attgcgctat tgccggaatt tattgttgca gatgaattag aaaataaaag tttgtttcaa
      781 atagacgtag atgtcgatat tccgaattta gaaacaacat tgataattaa tcctgaatcg
      841 aataagcaag tacttgaatc ttttgtaaaa gatgtttttt tataattatt ggtgaaaacg
      901 tgtagttatg gtgaaactca aagataataa tttaaatgag atgttaatga aaaagtaatt
      961 caatataaaa acaggtgatt tatatcttta ataaggatta tttctagttg aaatttcaaa
     1021 ttgcgggcat tcattttcaa aatagtaaag aatttaatag atgaaaacct ttgaatcata
     1081 ctagttagta tggattgatt aatattttaa cctgctaaat gagtagctta tgtattttaa
     1141 atagctgtca tctagcaggt tctttattat taataagttc agataaatta ttttagtctg
     1201 ttttctaatg ttgataataa taactttaaa tctgtacttt cattgtgaga tatatagtac
     1261 atcataaatt tttatatttg gattgagact ctttggaaga ttttcaataa atgaatattt
     1321 cacaaacttc cacttatcag gattataaat tataatttct gcaactgcat tatcgaagat
     1381 tctaaacaat tttttgattt ctatttcttt tagagataaa gtagggaaaa tgtcgatata
     1441 ttcttcaagc gcataaagac tatgtttaac agaatcggtt atgttcattg aatatatcac
     1501 accaatattt atattttgcc atccaaaaga agaaataaca ttatcataaa atccattgaa
     1561 tataaattca gtcatcccct ctgaacttct ccataaatat aatggtgaat aagtatttgt
     1621 aatgcaatta gtagttgttt gacttattaa ataagctttt ataattaaat ttttaaatcc
     1681 atcagtctta taaccattta atcgaactct atctctaatt atttccatat tgtaatcact
     1741 aggcaactta acatgatatt gcattgcatg catttagcat accccctttt ataaaaagga
     1801 tagcaataat aagtaaaatc tcatattatc caattgtgat atagttatca taaaaagtga
     1861 taggtgatta aattgaactt taatgatttg gaaattttta taactgtatg tgaagaagca
     1921 tctatcaata aagctgcaat taaacttaga tatgcacaat ctaatatatc tcaaagaatt
     1981 agcaagcttg aaaatgaatt aggtgtagtt ttgcttttta gaaatcaaaa aggtgctaag
     2041 gcaactaaag caggcgaaga attcttagcg tatagcaaaa aagtattaag agatacagag
     2101 actataaaaa ataaaatgaa aa
//
LOCUS       DBNENW010000244         2107 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNENW010000244 DBNENW010000000
VERSION     DBNENW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.248"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1653
                     /locus_tag="AC1LW8_002682"
     CDS             <1..1653
                     /locus_tag="AC1LW8_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIP4774428.1"
                     /translation="LDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKES
                     FISEIKSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVH
                     NSEIKFVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNL
                     TLQIHNKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELS
                     KEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDID
                     EIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGK
                     MNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLT
                     LENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRH
                     ISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLL
                     NLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(1724..2086)
                     /gene="mscL"
                     /locus_tag="AC1LW8_002683"
     CDS             complement(1724..2086)
                     /gene="mscL"
                     /locus_tag="AC1LW8_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIP4774429.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          842 a          281 c          341 g          643 t
ORIGIN      
        1 ttagataaac tattaggtag taagcaagag ttagagaatc aaattaatga agaaacatct
       61 atattgaaga atctcgaaat aaaattagat cgctacgata aaacaaaatt ggacttaaat
      121 gataaagaaa gctttataag tgaaattaaa tctgctgtaa atattggaga tcaatgtccg
      181 atatgtggta atgaaattca ggatttaggg catcatattg attttgacag tattgctaaa
      241 cgtcaaaatg aaattaaaga aattgaagca aatatccacg ctataaaatc gaatattgct
      301 gtgcataatt ctgaaattaa atttgttaat gaaaaaatat cgaatattaa tattaaaacg
      361 caaagtgatt tttcacttga agtattgaat aagcgtctgc tagaaaatga aaatgcattg
      421 aataatcaaa gagatcttaa taaatttata gaacaaatga aagaagaaaa agataatcta
      481 acgttgcaaa ttcataataa acaattgcgt ctaaataaaa atgaatctga gttgaaatta
      541 tgtcgagatc tcatcactga atttgaaaca ctctcgaaat ataataatat cactaatttt
      601 gaggtggatt ataagaagta tgttcaagat gtgaatcagc atcaagaact ctcaaaggag
      661 attgaagata agttaatgca attgtctcaa agaaagttaa ttgagcaaaa taatctaaat
      721 cattatgaaa atcaactaga aacttacaat aatgacttag aattgaatga acagtctatt
      781 gaaatggaaa tgtcgaggct gaatttaact gatgacaatg atatagatga aataatagcc
      841 tggaggggcg agcaagagga attagagcag aaaagggata cttataaaaa acgttatcat
      901 gaatttgaaa tggaaatagc taggttagaa tcattaacta aggataaaga gttattggac
      961 tctgataaat taaaagatga atatgagcta aaaaaaggaa agatgaatac actgatagat
     1021 gaatactctg ctgttcatta tcaatgtcaa aataatatta ataaaacaca atctatagtt
     1081 tcgcatatta attacttaaa tcaagaatta aaggatcaac aagaaatatt tcaattggct
     1141 gaaattgtca gtggtaagaa taacaaaaat cttacattgg aaaactttgt cttaatttac
     1201 tatttagatc aaattattgc tcaagcaaat ctgagattag caacaatgtc agataatcga
     1261 taccaactaa ttaggcgaga agcggtttct catggtctta gtggcctaga aattgatgta
     1321 tttgatttgc attcaaataa gtctagacat attagctcgt tatcaggtgg agaaactttc
     1381 caatcgtcgc ttgcattagc tttagggtta agcgaaattg tacagcagca atcaggaggt
     1441 atttcactag aatcaatatt tattgatgaa ggattcggta cattagatca agaaacgctt
     1501 gaaacagcgt tagacacttt attaaatctt aaatcaactg gtagaatggt tgggattatt
     1561 tcacatgtga gcgaattgaa aaatagaata cctttagttt tagaagtgaa atcagatcaa
     1621 tatcagagtt caacaagatt caaaagaaat taaaacataa aaaatagaga tggtactttg
     1681 gaatgtatag aacaacctta gcccatctct ttttatttaa aaattatttt ttctcacgta
     1741 ataaatctct gatttcagtt aataacacaa cattttcttc cacaactgct tcttcttcgg
     1801 cttcttcttt cttcattaat gtatttgcaa tcttaacaaa gatgaataaa gcaaacgcga
     1861 tgataataaa gtcgataaca gattggataa ataaaccgta tttaataccc cagaatgacc
     1921 attctttagc aaaatcaact gatccgaaaa ttttaccaat taatggcata atgatatttt
     1981 ctactaatga agatataatc ttgttgaaag ctgcacccat cacaacagca attgctaaat
     2041 ctaagacgtt accttttaag gcgaactctt tgaattcttt taacattaca ctcaacctct
     2101 ctttttt
//
LOCUS       DBNENW010000245         2072 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNENW010000245 DBNENW010000000
VERSION     DBNENW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.249"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(193..1647)
                     /locus_tag="AC1LW8_002684"
     CDS             complement(193..1647)
                     /locus_tag="AC1LW8_002684"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIP4774430.1"
                     /translation="MLMTKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGE
                     RLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIIESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDK
                     AKSIIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKKDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYT
                     LDKNNVPYKKEAGNYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI"
     gene            complement(1658..1960)
                     /locus_tag="AC1LW8_002685"
     CDS             complement(1658..1960)
                     /locus_tag="AC1LW8_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIP4774431.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAENKYRKATGQAPIKEVMTPTNMNDTNDLG"
BASE COUNT          623 a          417 c          281 g          751 t
ORIGIN      
        1 ttactggtaa tgtaattaca ttataacata aaaaaatagg caagtaccga agtacctgcc
       61 agttacgcac atttaaatct tgagagtaat gttaaaaagt gtataggaat attaacatcc
      121 atccaaatag ttatttaata actgtaagat tccctataat taatgtagca aaatttttat
      181 tctaagtaaa tactaaatcg tgctaaactt accaaaacta cttatcctat tacctgcttt
      241 atctacctct cctgtcgcga tataacgacg ttgtccacta ttagcaatat aagtaatcca
      301 tctatagcca ttgatacaat atgcaccgtc atatttgatt gttgcgttat taggtaatac
      361 accagtaatt cttgaattag ttgaatagcc gtcccttacg ttattacctt taacattggc
      421 aactgtgtaa ttaccagcct cttttttata aggcacatta ttcttatcaa gtgtataacc
      481 tgctggcact ggtggatttt tttggttttt agctgatgtt ttaacattac cagctaccaa
      541 accacctata ggcttaccat gaatcgcacc agctattaat ttagaataca agtcataatt
      601 cttcttaatc caatccatat cttttttatt agtaataaaa cctaattcag ataaacgata
      661 attgatattt atttctgctg atacattaac gttcagtaaa tcattacgag gtgttacacc
      721 tcttatttgt cctaagttat ttttaataac atcttgtata cttttatcaa tagtatctgc
      781 attgaattga cttgagataa taacatgccc accacttgcg ctttctcctg ctgcgtctaa
      841 atgtatttct agaacaatgt catacccctg tgatttaacc caatataagc cataatcttt
      901 tttattacct acattaacac cgtatgctgt atcttgatac atgtcttgtg attgacttga
      961 gccaccatat aatgcgactt catgaccggc atgtcttaaa tacttagcga tatttggcgt
     1021 tatatattta cgtataaaat cgcgttcgtt tgttccgttt cctactgctc caggatcgtt
     1081 ataaccatga ccggctacaa gcataatttt tttaggttta attactgctt gctttttggc
     1141 agttgcttgc ttaataatgc ttttagcttt atccccaaca cttactttgt cagggaaatt
     1201 taatctaata aaatacattg ggtcatcata ataatgaaca tgtcttgtaa cagtttcagg
     1261 accccaacca ggttgcgcaa cgccatttgt ccaaccttta ccattccaat tttggccaaa
     1321 cgatgtgaaa gtgtttaaat ttgcgctctc aataatttca acgtgtccag ctccgccacc
     1381 atacttcgac gggaaaacga caatgtccaa cttttgcggt aaaaagctat catagttttt
     1441 aattatttgc ccgtattttt caatccttgc tttattatca aatggaatat tataagcgta
     1501 taaaccttgt aacctttcgc ctgttgctat cataaaaaac atatttgcgt aatcgtaaca
     1561 ctgaaatcca taaaacaaat caggattgaa ctgcttccct aatgaattat caaaccattt
     1621 ttctgcttgg ttttttgtca ttaacataaa tcatcaccta ccctaaatca tttgtgtcgt
     1681 tcatattcgt aggtgtcatt acttctttaa ttggcgcttg ccctgttgct tttctatact
     1741 tgttttcagc tttatatttc tttagctttt gatttgccca tttaccttct tgagatgttg
     1801 gattatcttt atatgtagta tataaagcaa caactgttaa gataatcgat gaaacacttt
     1861 cttcatctac tggtatcgga cttatacctt tattcgctaa aaactgattg actaatgcta
     1921 agatcaatac gatgtatctt gttattactt ttgcatccat ttgtttgctc cttttatcca
     1981 aaatacaaag ccagtgccaa agcactgact cttaactatt acttacactt actaaaccag
     2041 aaacacgacc aaaagctata tcctaaaatt cc
//
LOCUS       DBNENW010000246         2062 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNENW010000246 DBNENW010000000
VERSION     DBNENW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.250"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            55..510
                     /locus_tag="AC1LW8_002686"
     CDS             55..510
                     /locus_tag="AC1LW8_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6978 family protein"
                     /protein_id="HIP4774432.1"
                     /translation="MAKLDLNSLNDEHVKLLINELKYPETHIDVNELKTIVASRINER
                     QEIISFKLGIKYLLTIKRGNIEKDRFSISIIFKDTYHTLVRIDINGGTHDNPDGTIAP
                     KSHIHIYNDKYDKKDRFAYEINLKDFPDIYNLYNVYMSFLEYNNIKDLE"
     gene            523..1311
                     /locus_tag="AC1LW8_002687"
     CDS             523..1311
                     /locus_tag="AC1LW8_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HIP4774433.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMITLTDDGYTIFDLENNGISLNKSKKRKKIFEEHLSAYGIKYNDKT
                     HEIFVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGR
                     DLPIIGSSGVVHNFDFFISAKKNQKEKFINAISNPNNSMIIKSKITDAMQAKKIKRHR
                     QNEFIFILNDSKKEINEHNKNLLHENYISTIDYSELDEKIGLLI"
     gene            1772..1982
                     /locus_tag="AC1LW8_002688"
                     /pseudo
     CDS             1772..1982
                     /locus_tag="AC1LW8_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
BASE COUNT          856 a          270 c          290 g          646 t
ORIGIN      
        1 aaataagaaa gtattaaaaa ttaatgccta tacaatgtta aaggaggaac agacatggct
       61 aaacttgatt taaatagtct taacgacgag cacgtaaaat tattaataaa tgaattaaaa
      121 tatccagaaa ctcatatcga tgtaaatgaa ttaaaaacaa tagttgctag tcgaataaat
      181 gaaaggcaag aaataataag ttttaagtta ggaataaagt acttattaac gataaaaaga
      241 gggaacatag aaaaagatag gttttcaatt tcaatcattt tcaaagatac ctatcacacc
      301 ctagttagaa tagatattaa cggtggtact cacgataatc cagatggaac aatcgctccg
      361 aaaagtcata ttcacatata taatgataag tatgataaaa aggataggtt tgcttatgaa
      421 attaacttga aagatttccc cgacatctat aacttgtata atgtgtatat gtcgttttta
      481 gagtataata atataaaaga ccttgaataa aggagggata atatgactac atttgatgct
      541 aaaaaattaa aaaaagaata tcttgattgg tataatcaga ccttagagtt ttctaattta
      601 tcaaacaatg tagtaagaat agatactcct tttaaagata attctttaga taatttaata
      661 atttacgctt tatacgatca gtccagagac atgattacac tgacagatga cggctatact
      721 atatttgatt tagaaaataa tggtatttct ttaaataaat caaaaaaacg taaaaagatt
      781 tttgaagagc acctttcagc ttacggtatt aaatataacg ataaaactca cgaaattttt
      841 gttcaaacta actttaaaaa ttttaataaa tcgaaacata atttattaca gtgccttata
      901 tttgttaatg atatgtactt actttctaat cctaagtcac agaacatatt tacagaagat
      961 gttgcaaaca aattggatga acataacatt tattacggaa gagacttacc tattatagga
     1021 agcagtggtg ttgttcataa tttcgacttt tttattagcg ctaagaaaaa tcaaaaagaa
     1081 aaatttatca atgctatttc taaccctaat aattctatga ttattaagtc gaaaataacg
     1141 gatgctatgc aagcaaaaaa aataaaaaga cacaggcaaa atgagtttat ttttatttta
     1201 aatgactcaa aaaaagaaat aaatgaacat aataaaaatc ttcttcatga aaactatatt
     1261 agtacaatag attatagcga attagatgaa aagataggtt tattgattta atatttatag
     1321 acgtgataat atcaatgttt atattaaatg aaacgaattt gaaaaattcg aaacagctta
     1381 agatagcaaa ttgaatggcc ttattgataa tgcagaatcg tctgcactta gttgaacaaa
     1441 ttctatgaga atagatattg ttaaactatg tggataggtg attttatttt gacaagagtc
     1501 agaagaacat atattaagga tttcaaatta caaatggtta aactttatga aaatggtata
     1561 acaagaatcg aaattatacg cgaatacgat ttaacactct caatcttctc gaactgaata
     1621 aatactgggt cattcaatca tcaagacaac ttattaagtg atgataaatg ttcataccat
     1681 tttgcaaata taaccagata ctctcaacaa aatgtagagt tcaaaattaa aaatgtccct
     1741 aattggcaca ttgctgtatg atattaaatt caacttagat ttcaacaata taactaatga
     1801 agcttatatt taccgcgata tcaaaccaaa cagcctatcc acaaacaaat tagaatatac
     1861 catgttagta atagtaaatg atatatatct tccaaagacc gcaaactgta gcagaaaatg
     1921 agaaccttgc gtttttgaaa tggtctaata aatcaaatat cattgaaact ttaatcacat
     1981 aaattaacag tagtcccctg ttcgcagttc tataaattac aaatgcaata aaggctaaag
     2041 taaactaatt actttagcct tt
//
LOCUS       DBNENW010000247         2062 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNENW010000247 DBNENW010000000
VERSION     DBNENW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.251"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            30..1031
                     /gene="argF"
                     /locus_tag="AC1LW8_002689"
     CDS             30..1031
                     /gene="argF"
                     /locus_tag="AC1LW8_002689"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIP4774434.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFRLVCPKELNPTDELLNRCKNIAAENGGNILITDNI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1054..1986
                     /gene="arcC"
                     /locus_tag="AC1LW8_002690"
     CDS             1054..1986
                     /gene="arcC"
                     /locus_tag="AC1LW8_002690"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIP4774435.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
BASE COUNT          772 a          284 c          403 g          603 t
ORIGIN      
        1 tgtaactaaa ataaaaggag ttgttattaa tgaaaaattt acgaaacaga agttttttaa
       61 ctttattaga cttttcacga caagaggtag aattcttatt aacactctcc gaagatttaa
      121 aacgtgctaa atatattggc actgaaaagc ctatgttaaa aaataaaaat attgcactgt
      181 tatttgaaaa agattctaca agaacgcgat gtgcatttga agttgcagcg catgatcaag
      241 gtgcaaatgt aacttattta ggcccaactg gatcacaaat gggtaaaaaa gaaacaacta
      301 aagatactgc acgtgtgctt ggtggaatgt atgatggcat tgaataccgt ggtttttcac
      361 aaagaacagt agaaacttta gctgagtatt caggcgtacc agtgtggaat ggtttaactg
      421 atgaagatca tcctactcaa gttcttgctg atttcttaac agcaaaagaa gtcttaaaaa
      481 aagattatgc agatattaac tttacatatg ttggagatgg tcgtaataac gttgcaaatg
      541 cattaatgca aggtgctgcc attatgggta tgaacttccg tttagtttgt ccaaaagaat
      601 taaatccaac agatgaatta ttaaatcgct gtaaaaatat tgccgctgaa aatggtggca
      661 acatattaat cacagataat attgaccaag gtgtaaaagg ttcggatgta atttacactg
      721 acgtttgggt atcaatgggt gaacctgatg aagtatggaa agaacgactt gaattattga
      781 aaccatatca agtaaataaa gaaatgatgg ataaaactgg taatccaaat gttatttttg
      841 agcattgctt accatctttc cataatgctg atacgaaaat tggtcaacaa atttttgaaa
      901 aatatggaat tcgagaaatg gaagttacag atgaagtatt cgaaagtaaa gcttcagttg
      961 tattccaaga agctgagaac agaatgcata caatcaaagc agtgatggtt gctacattgg
     1021 gtgaatttta aatgatataa ggaagtgaat atgatggcga aaatagtagt agcattaggt
     1081 ggtaatgctt taggaaaatc acctcaagaa caactcgagc ttgttaaaaa tactgcgaaa
     1141 tcattagtag gattaataac aaaaggacat gagattgtta ttagtcatgg taatggacca
     1201 caggttggaa gcattaattt gggacttaac tatgctgcag aacataacca aggtccggca
     1261 tttccatttg ctgaatgtgg cgcaatgagt caagcttaca tcggctatca attacaagaa
     1321 agcttacaaa atgaattgca ttctattgga atggataaac aagtggtaac actagtgaca
     1381 caagttgaag ttgatgaaaa tgatccggca tttaacaatc cttcaaaacc aattgggtta
     1441 ttttacaaca aagaagaagc tgaacaaatt caaaaagaaa aaggatttat atttgttgaa
     1501 gatgctggaa gaggatatag gcgcgttgtt ccttcaccac aacccatctc tattattgaa
     1561 ttagagagta ttaaaacact tattaaaaat gatacactcg ttattgctgc tggcggtgga
     1621 ggtataccag taattagaga gcaacatgat ggttttaaag gtattgatgc agttatagac
     1681 aaagataaaa caagtgcatt gttgggtgct aatattcaat gcgatcaatt gattatttta
     1741 acagcaattg attatgtata tattaatttt aacactgaaa accaacagcc tttgaaaaca
     1801 acaaatgttg atgaattaaa acgatatata gacgaaaatc aatttgcaaa aggaagtatg
     1861 ttaccaaaaa ttgaagcagc catatcattt attgaaaaca atccaaaagg aagcgtgctt
     1921 ataacatcat taaatgaatt agatgctgcc ttagagggta aagtaggtac tgtgattaaa
     1981 aagtaattga attgaaacgc ttttcaatta ctatatgtca aatgcatgat ttttattatt
     2041 tatgtgcacc ccctgaaaat aa
//
LOCUS       DBNENW010000248         2048 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNENW010000248 DBNENW010000000
VERSION     DBNENW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.252"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            95..322
                     /gene="elxI1"
                     /locus_tag="AC1LW8_002691"
     CDS             95..322
                     /gene="elxI1"
                     /locus_tag="AC1LW8_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epilancin biosynthesis-related protein ElxI1"
                     /protein_id="HIP4774436.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(628..1575)
                     /locus_tag="AC1LW8_002692"
     CDS             complement(628..1575)
                     /locus_tag="AC1LW8_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HIP4774437.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATSIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            1824..2012
                     /locus_tag="AC1LW8_002693"
     CDS             1824..2012
                     /locus_tag="AC1LW8_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIP4774438.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
BASE COUNT          786 a          256 c          349 g          657 t
ORIGIN      
        1 atttatcgtg gtaaaaatgt atttgctgta aacagttttt gtaacacatg atacatatgg
       61 ttgtgaaaat agcatatata aaggatggct ataaatgaca catttgacaa aggttttaga
      121 tacactaact ggaatatgcg tagtattatt atttagtaaa tattttgtgg cgtatgcaaa
      181 tatggtgttt gattggaatt taagatggta tttgctagaa aacataccac atttgccaat
      241 tatattattt attctgatgt ttattttcgg agtaccttct gaaatgataa aagataggca
      301 aaggaaaaat aacggcgttt aatttatcga tatttagagg tgataaaatt tgtcaactat
      361 tacaaagact aaaataaaaa tctttattaa ttattttatt ggtatattag ccttggcgtt
      421 tgctgcttat atattattta aataaaggtt agagtgaaac gtgtttatga actagacgtt
      481 ctagtatagt tactacagct ttagtcagaa ggtatcattg ataagatcat attaaatcaa
      541 agaggcattg atatacacta aaaagaggca agattacctg cctctttttt agttattaaa
      601 tatgcgtgtt aattcttggt aatagtgtta ctgagacgca ttacgaagaa atttatcttg
      661 tttttccttt tttaaaaaga agtgaaggta tcctataaag actctaagta ctattacagt
      721 ggctaataac acttcgataa acaaaagacc tttccaaatg tctggaaaca taagtacagg
      781 caaactgttc tttaaagcag ttgctgagat tactaaaggg aatgtgaaag ctgaaaatac
      841 gggcgaaaaa ggttctttta gtaatttagg caattgtata atgatataaa aataaaatat
      901 ttgagctaat attaaaaata taattacgat aaaagcattc gccttaggaa aggctataac
      961 atatgctgca gcgactaaag aaaatggtgc acaaattgtc gatgtgttcg gtttgattga
     1021 cgtctgcaat ggaaatgctt ttaatcgctt gaaaactata ggtaagacaa tagaagtagc
     1081 tacaaagcca tatattactg ttaattgacc tatgaaaaaa tatccgctaa cgggtgccgt
     1141 caatcctgcg atagcaatac caataaaaag tacagtccac gaaggataaa cattttcaag
     1201 tgaaaaatct tttaaatatt ttattgaaaa aataatcata tgcgtcataa ttcccacaag
     1261 gcataaaatc caaataggcg ttattaagct attgataaaa gttatgttac taaaaaatgt
     1321 atttaaataa gtagtaccta aaaagccaga catgaaaaat gttgtgaaca ctgatgaaac
     1381 tagaggactg tttaattgtt ctttcacatt attaaaatat ttgatcatag tacataaaag
     1441 gtgaatccaa atcaagaaag caaagattcc gcaaacagcg tttaaagtaa gagataagtc
     1501 ttttaataga ttacccaggc ctaataatcc taagactagt cctgacgtta ctagaggtgc
     1561 tttttgaagt ctcatgattt acaacctttc ttatgtgatt ttcttcacta attatatcat
     1621 gatagctatg gccaattaat aagaagagtg tgtactatta cgttattaga gtatgtatat
     1681 tggattgata gacacataat tgacatttaa atctcaaaat taatgatatt tgaggtatgc
     1741 tttgcaacct aaatattgga atatgtggaa aagtaattat ttaatttaaa tgatgattga
     1801 agatttacaa gggggtgtac aaaatgagaa atcaaattca aaaactatta gacagtgatt
     1861 tgagcagttt acatatatcg aaacaaacag gagttccaca aagcacaata cacagaatga
     1921 gaaaaaatga aagatcatta gacaatatgt cattgaaaaa cgctgaacta ctttataaat
     1981 ttgccaatag tatatttagc aatgaaaatt aagaagaata attagtgagt taaataatta
     2041 aaaaacca
//
LOCUS       DBNENW010000249         1965 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNENW010000249 DBNENW010000000
VERSION     DBNENW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.253"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            158..1072
                     /locus_tag="AC1LW8_002694"
     CDS             158..1072
                     /locus_tag="AC1LW8_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HIP4774439.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVYVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKEELDNLTEVLK
                     EYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDKSFRD
                     GMRNSKKQLKDKSRPYVVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPHDLNVSL
                     QISNKYINTTRPNYSKKDVIEVGVFNHE"
     regulatory      1286..1420
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1482..>1965
                     /locus_tag="AC1LW8_002695"
     CDS             1482..>1965
                     /locus_tag="AC1LW8_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP4774440.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     I"
BASE COUNT          731 a          274 c          340 g          620 t
ORIGIN      
        1 caattacaat tcaaataggt aagcctacta ttaatactaa aaaagccttt tatgatgata
       61 atcgtccaat agaatatggg gtgcacagta aaggtgaata aaattttaaa aaattgcttt
      121 taatattcta aacaaacaca taaatgggag atgagctatg tttaaaagaa ttaaactaat
      181 cttaatagca acgataatac tatcaggatg ttcaactacc aataacgaat ctaataaaga
      241 aacaaaatca gtaccagaag aaatggaagc ttcaaaatat gtaggtcaag gcttccaacc
      301 acctgcagaa aaagatgcga ttgaatttgc gaagaagcat aaagataaaa ttgctaaacg
      361 tggcgaacaa ttttttatgg ataacttcgg tctaaaagtt aaagctacga atgttgtagg
      421 tagtggcgac ggcgtagaag tatacgtgca ttgtgatgac cacgatatcg tatttaatgc
      481 gagtattcca tttgacaaat caattattga cagtgatagc tcattaagaa gtgaggataa
      541 aggcgatgat atgagtactt tagttggtac agtgttgagt ggctttgaat atcgagcaca
      601 taaggaagag ttggataatt taacagaagt tttaaaagaa tataaaagta aatataaata
      661 tactggttat actgaaaatg caattatgaa aacgcagaat agtggtttta gaaatgagta
      721 ttattatttg actgcaattc catatacttt agatgaatat aaaaagtatt ttcaaccctt
      781 aatcaaagag gatgataagt cttttaggga cggtatgaga aactcaaaaa aacaacttaa
      841 agataaatca cgtccatatg ttgttacaac tttatttagt actaaagata attttactaa
      901 agataataca atagacgaga tgattgattt ttctgaagtg ctaaaaaaga aaaagaatat
      961 tcctcatgat ttgaatgttt ctcttcaaat ttctaataaa tatataaata caacaagacc
     1021 taattatagt aagaaggatg taatagaagt aggtgtattt aatcatgaat aagctaacac
     1081 gaatgattaa actaaaaaca ataaaaagat aggttcaaaa aagccatctc aaaaattaat
     1141 caaacaaaca acattccaac caatgctcgc aaatcgcaaa ttcattatac atcaccaact
     1201 taattaattt tcattttaag catagttatt gaggttttgt gatatatagt ataaaattaa
     1261 tgggaattaa atttaataat gtaaaattca tcttcggggt cgggtgtaat tcccaaccgg
     1321 cagtaaatta agcctgcgac ctgctagtat gtatcatatt agtggctgat ctagtgagat
     1381 tctagagccg acagtatagt ctggatggga gaagatggag gttttttgtt gtgcaataat
     1441 cctcctattc ttacgagatg aatggaagga gaaaattgaa tatgcaacaa aataaacgtc
     1501 ttatcacaat aagtatgttg agcgcgattg cgtttgtgtt aacttttatc aagtttccta
     1561 taccattttt gccaccatac ttaactttag attttagtga tgtaccgtca ctactagcta
     1621 catttacgtt tggaccagtt gccggtatcg tagttgcact ggttaaaaat ttattgaact
     1681 acttatttag tatgggcgat ccagttggac catttgctaa ctttttagca ggcgcaagtt
     1741 tcttattaac tgcttacgcc atctataaaa ataaacgttc aacaaaatct ttgattactg
     1801 gattaatcat tgcaacaatc gttatgacta tcgtgttgag tattttgaac tatttcgttc
     1861 tattaccttt gtacggtatg atatttaact tagctgatat cgcaaataat cttaaagtaa
     1921 tcattgtttc aggaattata ccattcaata ttattaaagg tatcg
//
LOCUS       DBNENW010000250         1953 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNENW010000250 DBNENW010000000
VERSION     DBNENW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.254"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            78..1784
                     /gene="acsA"
                     /locus_tag="AC1LW8_002696"
     CDS             78..1784
                     /gene="acsA"
                     /locus_tag="AC1LW8_002696"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIP4774441.1"
                     /translation="MKVEVYKGAQGKHNLKDYEEIYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
BASE COUNT          678 a          244 c          442 g          589 t
ORIGIN      
        1 aaattttgaa taattagaaa tgttatgtat aatattgaga aagaaagcgt tttcacataa
       61 caaaggggga gtttcaaatg aaagtcgaag tttataaagg agcgcaaggt aaacataacc
      121 ttaaagatta tgaagaaata tataatactt ttgattggaa agacgtagaa caagcatttt
      181 cttggagtga aactggaaaa atgaacatgg catatgaatg tatagatcgc catgtagatc
      241 aaggattagg ggataaaata gcgttaaatt acaaagatga gtacagaaaa gaatcgtata
      301 cttataaaga tatgcaacgg ttatctaata aagcagcgaa tgttttgtct gaacatgcag
      361 aagttgacaa aggtgacaga gtatttatat ttatgtcgcg tacacctgaa ctatattttg
      421 cgttgttagg tgttttaaaa attggtgcaa ttgttgggcc gttatttgaa gcatttatgg
      481 aaaaggcagt tgcggataga ttagagaaca gtgaagctaa agtgttaatt actaataagg
      541 cattgttacc tcgagtacct gtagataaat taccgaactt gaaaaaaatt gttgtcgtag
      601 atgaggatgt agaagacaat tacatagact tcattagttt gatggaaact gctagcgatg
      661 aatttgacat tgaatggtta aagtcggatg atggtttgat tttacattat acatcaggtt
      721 ctactgggca acctaaaggt gtattgcatg ttcaacaagc aatgttagtg cactatattt
      781 ctggaaaata tgtattagat ttacaagaag atgatgttta ttggtgtaca gcagatccag
      841 gttgggttac aggaacatct tatggtattt ttgcaccatg gttaaatggc gctacaaatt
      901 gtatagctgg tggtcgcttt tcaccagaac agtggtatag tatgattgaa gattttaaag
      961 tgacgatttg gtatacggca ccaacagctt taagaatgtt aatgagtgct ggtgacgata
     1021 ttgttgagaa atatgacttg tcatcgttac gttcgattct atcagtaggt gagcctttaa
     1081 atcctgaagt tataaaatgg gcgaaaaaag tatacggttt aacggtgtta gatacttggt
     1141 ggatgacaga aacaggtgga catatgattg ttaactatcc aacgatggac gtcaagcttg
     1201 gctcaatggg caaaccatta cctggtattc aagctgcaat tatcgatgat gcaggtaatg
     1261 aattaccacc aaatcgaatg ggcaaccttg ctataaaaaa aggctggcca tcaatgatgt
     1321 atcgtatctg gaagaatcca gaaaaatata agtcatattt tattggagac tggtatgtat
     1381 ctggtgattc ggcatataaa gatgaagatg gttacttctg gttccaagga cgtgttgatg
     1441 atgtaattat gacagctggt gaacgagttg gaccatttga ggttgagtct aaattggttg
     1501 aacacgaagc agttgccgaa gcaggaatta ttggtaaacc tgatccggtt cgcggtgaaa
     1561 taattaaggc gtttgttgca ctgagaaaag gatatgaacc aacagacgaa ttaaaagaag
     1621 aaattcgttt atttgttaaa gaaggtttgt cggcacatgc agcaccacgt gaaatcgaat
     1681 ttaaagataa attacctaaa acacgctcag gtaaaattat gagacgtgta ttaaaagctt
     1741 gggaattaaa tttagatgca ggggatttaa gtacaatgga ataatgacat gaatgttatt
     1801 gaagatttct ttcgaagaat aaagggatac aacatatttc atgttaatgt ttaaatatta
     1861 gtttacttta tgataagcaa tataaagagc tgttaacttg tgtcatatca tttcgtagaa
     1921 agcatttgaa aatgatgaca taacaataat ggc
//
LOCUS       DBNENW010000251         1923 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNENW010000251 DBNENW010000000
VERSION     DBNENW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.255"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(287..>1923)
                     /locus_tag="AC1LW8_002697"
     CDS             complement(287..>1923)
                     /locus_tag="AC1LW8_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIP4774442.1"
                     /translation="SPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTNNIKM
                     WITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFSAGMG
                     IGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALALAYS
                     QFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLH
                     YLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGP
                     TVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVGVF
                     IARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGV
                     FNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQAL
                     IAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLGLTLT
                     PNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          690 a          378 c          286 g          569 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aag
//
LOCUS       DBNENW010000252         1894 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNENW010000252 DBNENW010000000
VERSION     DBNENW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.256"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            54..>1894
                     /gene="cna"
                     /locus_tag="AC1LW8_002698"
     CDS             54..>1894
                     /gene="cna"
                     /locus_tag="AC1LW8_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HIP4774443.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEALAARDISSTNVTDLTVS
                     PTKIEDGGKTTVKMTFDDKSVKIQNGDTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIITDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     EDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGE
                     KVKTLDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGT"
BASE COUNT          799 a          250 c          374 g          471 t
ORIGIN      
        1 aatttatcat tagtatccta tggatatgac atagagtata aggaggggtt tttatgaaca
       61 agaacgtgtt gaagtttatg gtctttataa tgttattaaa tatcatcacg cctttattta
      121 ataaaaatga agcattggca gcacgagata tttcatcaac gaatgttaca gatttaactg
      181 tatcaccgac taagatagaa gatggtggta aaacgacagt aaaaatgaca ttcgacgata
      241 aaagtgtaaa aatacaaaat ggtgacacga ttaaagtggc atggccgaca agcggtacag
      301 taaagataga gggttatagt aaaacagtac cattaactgt taaaggtgaa caggtgggtc
      361 aagcagttat tacaccagac ggagcaacaa ttacattcaa tgataaagta gaaaaattaa
      421 gtgatgtttc gggatttgca gaatttgaag tacaaggaag aaatttaacg caaacaaata
      481 cttcagatga caaagtagct acgataacat ctgggaataa atcaacgaat gttacggttc
      541 ataaaagtga agcgggaaca agtagtgttt tctattataa aacgggagat atgctgccag
      601 aagatacgac acatgtacga tggtttttaa atattaataa tgaaaaaaga tatgtatcga
      661 aagatattac tataaaggat cagattcaag gtggacagca gttagattta agcacattaa
      721 acattaatgt gacaggtaca catagcaatt attatagtgg atcaaatgca attactgatt
      781 ttgaaaaagc ttttccaggt tctaagataa ctgttgataa tacgaagaac acaattgatg
      841 taacaattcc acaaggctat gggtcatata atagtttttc aattaactac aaaaccaaaa
      901 ttacgaatga acagcaaaaa gagtttgtta ataattcaca agcttggtat caagagcatg
      961 gtaaggaaga agtgaacggg aaatcattta atcatactgt gcacaatatt aatgctaatg
     1021 ccggtattga aggtactgta aaaggtgaat taaaagtttt aaaacaggat aaagatacca
     1081 aggctcctat agctaatgta aaatttaaac tttctaaaaa agatggatca gttgtaaagg
     1141 acaatcaaaa agaaattgag attataacag atgcaaacgg tattgctaat attaaagcgt
     1201 tgcctagtgg agactatatt ttaaaagaaa tagaggcgcc agcaccgtat acatttgata
     1261 aggataaaga atatccgttt actatgaaag atacagataa tcagggatat tttacgacta
     1321 ttgaaaatgc aaaagagata gaaaaaacaa aagatgtatc tgctcaaaag gtttgggaag
     1381 gcactcaaaa agtgaaacca acgatttatt tcaagttgta caaacaagat gacaatcaaa
     1441 acacaacacc agtagacaaa gcagagatta aaaaattaga agatggaacg acaaaagtga
     1501 catggtctaa tcttccagaa aatgacaaaa atggcaagac tattaaatat ttagttaaag
     1561 aagtaaatgc tcaaggtgaa gacacaacac cagaaggata tactaaaaaa gaaaatggtt
     1621 tagtggttac taatactgaa aaaccaatcg aaacaacatc aattagcggt gaaaaagtat
     1681 gggacgacaa agacaatcaa gatggtaaga gaccagaaaa agtcagtgtg aatttattgg
     1741 ctaatggaga gaaagtaaaa acgttagacg tgacatctga aacaaactgg aagtacgaat
     1801 ttaaaaactt accgaagtat gatgaaggaa agaaaataga atatacagtg accgaagatc
     1861 acgtaaaaga ctacacaaca gacatcaacg gtac
//
LOCUS       DBNENW010000253         1893 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNENW010000253 DBNENW010000000
VERSION     DBNENW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.257"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..264
                     /locus_tag="AC1LW8_002699"
     CDS             <1..264
                     /locus_tag="AC1LW8_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774444.1"
                     /translation="LSIYNNRSSNGKIMIFPSFKNLCFVLFSYFFCGGLSIMFLDVLI
                     SLSIQNIIVFIAWVIMTMLFFFINMSSIIDKKIHVIYLRSYKY"
     gene            352..969
                     /locus_tag="AC1LW8_002700"
     CDS             352..969
                     /locus_tag="AC1LW8_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774445.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVISTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            1044..1673
                     /locus_tag="AC1LW8_002701"
     CDS             1044..1673
                     /locus_tag="AC1LW8_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HIP4774446.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCVILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            1730..1846
                     /locus_tag="AC1LW8_002702"
     CDS             1730..1846
                     /locus_tag="AC1LW8_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774447.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
BASE COUNT          698 a          218 c          291 g          686 t
ORIGIN      
        1 ttatctatct ataataatcg gagtagcaat ggaaaaatta tgatttttcc atcattcaag
       61 aatctttgct ttgtactatt ttcttatttt ttctgtggtg gattatcaat catgttctta
      121 gatgttttga ttagtttatc tatccaaaat attatagtat ttattgcttg ggttattatg
      181 acaatgctat ttttctttat aaatatgtct tcaataatag acaaaaagat tcatgttata
      241 tatttaaggt catataaata ttaattttga tacaaaaggg cacaagtgtt ttgagaaaca
      301 gaaacgataa tatagctgtg tgaatttgtt attaatagga agtgataatt attgctttgt
      361 gaaaccgaaa ttatcaataa aaatccgaaa tacagagtta ttaagtatga tgatgagtat
      421 ttaatggtcg atgtaataag cacttggctt gtatactttt ttccatttat taattggttc
      481 attccaaaaa ggtgcgccaa aattagtaga gaagagtatg aaaaactgaa cactgttaag
      541 ccagttaaaa ataaaaattt ttggcctgtt gtaggaggaa caatcctact aggtgctact
      601 tccagaaagt acatacactt acttaatatt caattagaaa aaagatcggt tatatttata
      661 tgcttcgttg tatttctatg tattttaatc ttttttgtgc ttctgaatcg aaaattaaaa
      721 ttgaaagttt tcgataataa aaaagaagaa caaaaaataa ttttagtacc aacattaaaa
      781 aatgctgttc ttatattata tggctatttg ttgattggag gcatgtcaat attagcttta
      841 agcatgttgc tgacattaga aaaccagaat ttaataactt ttatagcttg gggtatgggg
      901 ttaatgttgt ttttcttgat gaatataaca ctcattgtca ataaaacagt caaagttata
      961 aaaaggtgaa taaataacta attatattgt attggccgtt cttcaaataa aaaataattt
     1021 gaataaggtg gaagtgataa acagtgcttt gcgaatctag agtcattaat caaaacccta
     1081 aatatagaat tattaaatac aataatgaat atttcatggt tgatttagta agtacttgga
     1141 ttgcttattt tttgcccatg attaattggt ttattcccaa aaagtacgcg aaaattagta
     1201 gagaagaatt tgaaagttta aatattgtca aacccgctaa aaataatact ttctggcctg
     1261 ttgcaggatt tgcagtgtta ttaacaacct taacaagaaa atatatctat ttgcttaaca
     1321 tccatttaga aaaagaaata gttatattaa catgctgtgt gatacttcta ggtgttttcg
     1381 cattgtttat atatataaat acaaaattga agttacatat ttttgataaa aataaaagta
     1441 ataacgaaaa gatcatatta atacctacat ttaaaaatat ttgtttatcc ttatttgctt
     1501 atatattatt tggtggattg tcaacaatgg ctctgagtat gttagtaact tcatcccctc
     1561 aaaatataat agaatttctt gctttaattg gcatgactgc atgcttcttt ctactgaata
     1621 tgtcatcggt tctagataaa aaaattcatg ttattttaaa aacaaataag tagtaaaatt
     1681 gattaactta ggtagtatcg gatacttaaa tgttggttca taaaaagcaa tgattttaaa
     1741 tcgaggagct atcttagaac agggaaataa aacagccaaa gttataaaaa gtgaattaat
     1801 aactaattat attatgttag ccacgcttca aataaaaaat aattagaata aggtgggatt
     1861 gataatcaat gctatgcgaa tctaaaatca tca
//
LOCUS       DBNENW010000254         1888 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNENW010000254 DBNENW010000000
VERSION     DBNENW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.258"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..870
                     /locus_tag="AC1LW8_002703"
     CDS             <1..870
                     /locus_tag="AC1LW8_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIP4774448.1"
                     /translation="ILVTVWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQ
                     NVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYII
                     SHIKIKHIEIYNKGYSSNTLMLLSKLSHKYNIKLIDVRQVSSFKLGDSSFLFFDSFIP
                     NSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSS
                     SKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
                     KVDSNSYGNASGL"
     gene            927..>1888
                     /gene="holA"
                     /locus_tag="AC1LW8_002704"
     CDS             927..>1888
                     /gene="holA"
                     /locus_tag="AC1LW8_002704"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIP4774449.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVNQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELF"
BASE COUNT          776 a          206 c          317 g          589 t
ORIGIN      
        1 atattggtta cggtttggac tataattatt ctgacattat taataacgtt tccaacaaat
       61 tcacatcaca aaattacaat gttaaatgtg gggcagggag acagtatttt atatgaaggt
      121 ggtaagaacc aaaatgtctt gattgataca ggtgggaaag tgattgatga tactaaacaa
      181 cctagttatt caatttctaa atatcatatt ttaccaacgc taaatgaaag agggataaat
      241 gaattagagt atctaatttt aacacatcca cacaatgacc atattggtga agtggaatat
      301 attattagtc atattaaaat taaacatata gagatataca ataagggata tagtagtaat
      361 acattgatgt tattatcgaa attaagccat aagtacaaca ttaaacttat agatgtaaga
      421 caagttagta gttttaaact tggagatagt agttttctat tttttgatag ttttattcca
      481 aatagccgag ataaaaatga gtattcgatt attactatga ttacatatca aaataaaaaa
      541 gttttattaa tgggcgatgc tagtaaaaat aatgaatctt tactactaaa aaaatataac
      601 ttgccggaga ttgatatttt aaaagtaggt catcatggga gcaagacaag tagttctaaa
      661 gaatttatag agatgattaa gcctaaaata agtttgattt cttctgggaa gaacaatatg
      721 tatcatcttc ctaatataga agttgttaaa cgattgcaaa ggattcgcag tcgcatttac
      781 aatagtcaac aaaacggtca agttacaatt gacttagatg ataatttaaa agttgattca
      841 aactcttatg gaaatgcaag tggtttatag atacagaaac atgatataat ctattgatgt
      901 gtcaaacgaa tgaaagggtg taatgaatga gcgacaatat tgtagctatt tatggagatg
      961 tgcctgaatt ggttgaaaaa caaagtgcag aaattatatc acaatttttg aaaagtgata
     1021 gagatgactt taactttgtg aaatataatt tatacgaaac agagattgca ccaattgttg
     1081 aagaaacatt aacattgcct ttcttttcag ataaaaaagc aattttggtt aaaaatgcat
     1141 atatatttac aggtgaaaaa gcgccaaaag atatggctca taatgtaaat caattaatag
     1201 aatttattga aaaatatgat ggcgaaaatt tgattgtctt tgagatatat caaaataaac
     1261 ttgatgaaag aaaaaagtta actaaaactc taaaaaagca tgcaaggctt aaaaaaatag
     1321 agcaaatgtc tgaagaagaa ataaaaaaat ggattcaaag taaattaaat gagaatttca
     1381 aagatatcaa aagagatgca ttagatttat ttattgagtt gacaggtatt aactttaata
     1441 ttgtctcaca agagatagaa aagttgattt tatttttagg cgatagacca acaattaata
     1501 agcaggatgt taaccaaatt attaatagaa gtttagagca aaatgtattt ttactgactg
     1561 aatacattca gaaaagaaag aaagaacaag caattcattt agtaaaagat ttaataacta
     1621 tgaaagaaga acctattaaa ttacttgcac taatcacaag taattaccga ttattttatc
     1681 aatgtaagat tttgagtcaa aaaggatata gtggacagca aattgctaaa acaataggcg
     1741 tacatccata cagagtaaaa ttagcgttag gacaagtaag acattatcaa cttgatgaat
     1801 tattgaatat tatagatgct tgtgcggaaa ctgattataa acttaaatca tcatatatgg
     1861 ataaacagtt aatactggaa ttattcat
//
LOCUS       DBNENW010000255         1887 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNENW010000255 DBNENW010000000
VERSION     DBNENW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.259"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            162..1721
                     /gene="rny"
                     /locus_tag="AC1LW8_002705"
     CDS             162..1721
                     /gene="rny"
                     /locus_tag="AC1LW8_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIP4774450.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT          724 a          266 c          382 g          515 t
ORIGIN      
        1 aatagtagaa atagcattca atatagtgca aaagtgcaaa ttgataactt gacacttatc
       61 tcctataaac cgtacaatta atttgtatga tttatatata atttcataaa gtcatattga
      121 atttcatata aagagcaaac cctagaaaag gaggtgtttg tgtgaattta ttaagcctcc
      181 tactcatttt gctggggatc attctaggag ttgttggagg gtatgttgtt gcccgaaatt
      241 tgttgcttca aaagcaatca caagctagac aaactgccga agatattgta aatcaagcac
      301 ataaagaagc tgacaatatc aaaaaagaga aattacttga ggcaaaagaa gaaaaccaaa
      361 tcctaagaga acaaactgaa gcagaactac gtgaaagacg tagtgaactt caaagacaag
      421 aaacccgact tcttcaaaaa gaagaaaact tagagcgtaa atctgatcta ttagataaaa
      481 aagatgagat tttagagcaa aaagaatcaa aaattgaaga aaaacaacaa caagtagatg
      541 caaaagagag tagtgttcaa acgttaataa tgaagcatga acaagaatta gaacgcatct
      601 ccggtctcac tcaagaagaa gctattaatg agcaacttca aagagttgag gaagaactgt
      661 cacaagatat tgcagtactt gttaaagaaa aagaaaaaga agctaaagaa aaagttgata
      721 aaacagcaaa agaattatta gctacagcag tacaaagatt agcagcagat cacacaagtg
      781 aatcaacggt atcagtagtt aacttaccta atgatgagat gaaaggtcga atcattggac
      841 gagaaggacg aaacatccgc acacttgaaa ctttaactgg cattgattta attattgatg
      901 acacaccaga agcggttata ttatctggtt ttgatccaat aagaagagaa attgctagaa
      961 cagcacttgt taacttagta tctgatggac gtattcatcc aggcagaatt gaagatatgg
     1021 tcgaaaaagc tagaaaagaa gtagacgata ttattagaga agcaggtgaa caagctacat
     1081 ttgaagtgaa cgcacataat atgcatcctg acttagtaaa aattgtaggg cgtttaaact
     1141 atcgtacgag ttacggtcaa aatgtactta aacattcaat tgaagttgcg catcttgcta
     1201 gtatgttagc tgctgagcta ggcgaagatg agacattagc gaaacgagct ggacttttac
     1261 atgatgttgg taaagcaatt gatcatgaag tagaaggtag tcatgttgaa atcggtgtag
     1321 aattagcgaa aaaatatggt gaaaatgaaa cagttattaa tgcaatccat tctcatcatg
     1381 gtgatgttga acctacatct attatatcta tccttgttgc tgctgcagat gcattgtctg
     1441 cggctcgtcc aggtgcaaga aaagaaacat tagagaatta tattcgtcga ttagaacgtt
     1501 tagaaacgtt atcagaaagt tatgatggtg tagaaaaagc atttgcgatt caggcaggta
     1561 gagaaatccg agtgattgta tctcctgaag aaattgatga tttaaaatct tatcgattgg
     1621 ctagagatat taaaaatcag attgaagatg aattacaata tcctggtcat atcaaggtga
     1681 cagttgttcg agagactaga gcagtagaat atgcgaaata atttttgtct ccctcacaaa
     1741 ttagtgaggg agctttttta agttgtagtc ttaatctagt tagacagcac tttatcggta
     1801 ataactatat taaacagtag ttatttgaaa gtaagacgga ccttatatta aataagaagt
     1861 tattgctttt aataaaaatg ttttagg
//
LOCUS       DBNENW010000256         1873 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNENW010000256 DBNENW010000000
VERSION     DBNENW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.260"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(55..570)
                     /locus_tag="AC1LW8_002706"
     CDS             complement(55..570)
                     /locus_tag="AC1LW8_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774451.1"
                     /translation="MAGIIKEISKQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            761..1765
                     /locus_tag="AC1LW8_002707"
     CDS             761..1765
                     /locus_tag="AC1LW8_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIP4774452.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDFPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
BASE COUNT          632 a          308 c          309 g          624 t
ORIGIN      
        1 gtatacacaa tatttattac tatgatttca ttttatctgc tgcaaaaaca atcattataa
       61 ctctttttcc ataattaaat ctgtatccgt tacatcacct gtttgaaaat gatgttcacc
      121 aaccacttta aatccatgac gtttataaaa tgcttgagca cgaggattat gttcccaaac
      181 tcctagccaa attttatgtt tattatgttc ttgtgcaatt ttttcggcca attctatcaa
      241 ttgtgaacct cttccgccac cttgaaagtc tttcaaaaaa tatatgcgct gcacttctaa
      301 ataggtctcc cccatttctt cagtttgagc actattaata ttcatcttta tataaccaac
      361 attcgcacca tcttcttgat aaaaataatg aaatgaatct acatggttaa tctcttgtgt
      421 aaatttttct acagtataat tgtctttaaa aaattgatca aaatctttgt catcatagta
      481 agaaccaaac gtgtcataaa atgttctagt tgctaattca actaattcac tagcattttg
      541 ttttgaaatt tctttgatta tcccagccat ataaatcctc caataaacag tgatcgaatc
      601 aaaatattac ttatgttatt tttcagccaa aactatttaa aaatacatta acacaaatca
      661 attacagatt gtattgattg tgtgtaacat caataaatga tacatttatt ccagtaaaat
      721 ggccgtattt tcaaaagaga aaaagagagg atgtatcgtt gtgatagaaa catttaaagc
      781 atttgtaatt gataaagatg agagcggtaa agtgacacca actttcaaac aattatcgcc
      841 tactgatttt cctaaaggag atgtgctgat taaagtacat tactctggta taaattataa
      901 agatgcttta gcgactcaag atcataatgc agtcgtaaaa tcgtatccta tgattccagg
      961 aatagattta gctggaacaa ttgttgaatc cgaagcacca ggctttgaaa aaggagaaca
     1021 agtaattgta acgagttatg acctaggtgt tagccattat ggcggtttta gtgaatatgc
     1081 gcgtgtaaaa tcagaatgga ttatcaagct tcctgatact ttaacattag aagaatcaat
     1141 gatatatggc acagctggtt atactgccgg tttagcaatt gaaagacttg aaaaagttgg
     1201 aatgaatatt gaagatggtc ctgtactcgt tcgcggtgct tcaggtggtg tcggtacttt
     1261 agcagtactc atgcttaatg aacttggtta taaagttatc gcaagtacag gtaaacaaga
     1321 tgttagcgat caattacttg aacttggtgc caaagaagtt atcgatcgac ttcctgttga
     1381 agatgatcat aaaaagccac tcgcatcatc aacttggcaa gcttgtgtag accctgttgg
     1441 tggcgaaggt attaattatg ttacaaagcg tttaaatcat agtgggtcaa ttgcagttat
     1501 tggtatgact gccggtaata cttatactaa ttctgtattc cctcacattt taagaggtgt
     1561 aaacatttta ggaattgact cggtatttac tgctatgaaa ttaagacagc gcgtttggcg
     1621 tcgtctcgca aaagatttaa tgcctgaaaa tttacatgag atcaagcaag ttattacatt
     1681 tgatgaactt ccagaacaac ttaacaaagt aattaaacat gaaaataaag ggcgcattgt
     1741 tatcgatttc ggtgtagata aatagtattc atgaaaaaga catcccgtta tgcgagatgt
     1801 ctttttattt tcttttagat tatctactta atcaacatta cacaaattaa tcaataaaag
     1861 aattaaataa tta
//
LOCUS       DBNENW010000257         1863 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNENW010000257 DBNENW010000000
VERSION     DBNENW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.261"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            465..1589
                     /locus_tag="AC1LW8_002708"
     CDS             465..1589
                     /locus_tag="AC1LW8_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIP4774453.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNTYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(1670..>1863)
                     /locus_tag="AC1LW8_002709"
     CDS             complement(1670..>1863)
                     /locus_tag="AC1LW8_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP4774454.1"
                     /translation="EGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKI
                     VANAAISAVQGEKPQFVVN"
BASE COUNT          659 a          296 c          345 g          563 t
ORIGIN      
        1 gtcccacccc aacttgcatt gtctgtagaa attgggaatc caatttctct atgttggggc
       61 cccgccaact tgcacattat tgtaagctga cttttcgtca gcttctgtgt tgggaccccc
      121 accccaactc gcattgcctg tagaatttct tttcgaaatt ctctgtgttg gggcccctga
      181 ctagagttga aaaaagcttg ttgtaagcgc attttcattc agtcaaatac tgccaatata
      241 atattataga ccctaggaca ttgatttatg tcccaagctc ccacatcatt gttacaaatg
      301 tacatgatgt aaatgaatat tgctgtctaa atgtgcatgt aatatacaat ggtgcagata
      361 atacacttaa gtccttaaaa atgaaacgtt agttccaaga gtcattttta aacaatagtg
      421 catgtgataa aatagaaaag aatgaaaaat atagaggtga caatatgaag atagcaatta
      481 taggtgcagg catcggtgga ttaacagctg ctgcattatt acaagaacaa ggtcatacta
      541 ttaaagtctt tgaaaaaaat gagtcagtta aagaaattgg cgctgggatt ggtatcggag
      601 ataatgtgct taaaaaacta ggtaatcatg acttagctaa aggtattaaa aatgctgggc
      661 aaatcttatc tacaatgaca gtgttagatg acaaagatcg cctgttaact actgttaaat
      721 taaaaagtaa tacattgaat gtgacgttac cacgccaaac attaattgac attattaaat
      781 cttatgtaaa agatgacgca atatttacaa atcatgaagt cacgcatata gataatgaga
      841 cagataaagt taccatacat ttcgcggaac aagaaagtga agcatttgat ttatgtattg
      901 gtgctgatgg aattcattct aaagtgagac aatctgtaaa tgctgacagt aaagtattat
      961 atcaagggta tacatgcttt agaggtttaa ttgatgatat tgatttaaag catccggatt
     1021 gtgcaaaaga atactgggga agaaaaggaa gagtaggtat tgttccgtta ttaaataatc
     1081 aagcatattg gttcattaca attaactcga aggaaaacaa tcataaatat agttcgtttg
     1141 gtaaacctca tttgcaagca tactttaatc actatccaaa tgaagttaga gagatcttag
     1201 acaaacaaag tgaaacaggt atcttattgc ataatattta tgatttgaaa ccactcaaat
     1261 cttttgttta tggtcgtact attttactag gagatgcagc acatgcgaca acgcctaata
     1321 tggggcaagg tgctggacaa gcaatggaag atgctatcgt attagtaaat tgttttaaca
     1381 catacgactt tgaaaaagca ttacagcgtt atgataaaat acgtgtcaaa catactgcaa
     1441 aagtaattaa gcgttctaga aaaatcggta aaattgccca atatcgtagt cgtttagttg
     1501 ttgcagttag aaatcgtatt atgaaaatga tgccaaatgc attagcagct ggacaaacta
     1561 aattcttata taaatcgaaa gagaaataat acaacaatat gaaaaccccc gtatgttgaa
     1621 acgagagctc aacatatggg ggttcttgtt tttataataa attcaattat tagttaacga
     1681 caaattgtgg tttctcacct tgaacggcac taattgcagc attagcaaca attttagaca
     1741 tcatgtcacg tgcttcaaat gtagcattac caatatgcgg tgttaatact acattattaa
     1801 gtgattttaa gtcatcggta atatctggtt caaattcata tacatcaagt gcagcacctt
     1861 caa
//
LOCUS       DBNENW010000258         1862 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNENW010000258 DBNENW010000000
VERSION     DBNENW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.262"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..246
                     /gene="lpl5"
                     /locus_tag="AC1LW8_002710"
     CDS             <1..246
                     /gene="lpl5"
                     /locus_tag="AC1LW8_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HIP4774455.1"
                     /translation="SINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHID
                     GDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            278..1069
                     /locus_tag="AC1LW8_002711"
     CDS             278..1069
                     /locus_tag="AC1LW8_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774456.1"
                     /translation="MEYLKRLALFISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKPGEMDSEGMVL
                     HLNRNMRTATGHYTIRTTYDELGKMTREKNYRVELKNNKIVVLDKVEDVNLKHKIENF
                     KFFSQYADFKDLKNYKNGRISINENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYMFSKEKEDETFMSDFLNFGPSHSN"
     gene            1121..>1862
                     /locus_tag="AC1LW8_002712"
     CDS             1121..>1862
                     /locus_tag="AC1LW8_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774457.1"
                     /translation="MGYFKRVLLYIIVMVLSVFIIGCDKSSDTSEKSKGDSKEAQIKK
                     SFAKTLDMYPTENLEDFYDKEGYRDGKFKKGDKGTWVIRSEMTTELKNENMVSKGMVI
                     RLNRNSRTCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIIPLKPIDDEKVKKEIE
                     EFKFFVQYGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTQKA
                     PKLLLKGSGNLKGSSVGYKNIEFTFVENK"
BASE COUNT          788 a          198 c          364 g          512 t
ORIGIN      
        1 tcaataaatt ggaatgttcc aagttatgac gtggaatata aaatgagcaa taaagatgaa
       61 aatgttaaac aattaagaag tcgttataac attcctactg ataaagctcc aatgttaaaa
      121 atgcatattg acggggactt aaaaggtagt tctgttggat ataaaaggtt agaaatagat
      181 ttttcaaaag aagataggga tatttcagtc attgattatt taagttataa gccagcgaaa
      241 aaatagtact gataattaaa tattagggtg tgaaggaatg gaatatctaa aaaggcttgc
      301 attgttcata agtgttatta ttttgaccat ttttataatg ggttgtgata gtcaaagcga
      361 tactgcagaa aatccaaaag aaggttcaaa agaagcacaa attaaaaaga gtttttcgaa
      421 aacgttagat atgtatccaa ttaagaatct cgaggattta tatgacaaag aaggatatcg
      481 tgatggcgaa tttaaaaaag gtgataaagg gacgtggaca atatcaacag attttgctaa
      541 aagcaacaaa ccaggtgaaa tggatagtga aggtatggtg ctacatttga atagaaatat
      601 gaggacggca actgggcatt atactataag aacaacttat gatgaattgg gtaagatgac
      661 acgcgaaaaa aattatcgtg ttgaacttaa aaataataag atagtagttt tagataaagt
      721 agaagatgta aaccttaaac ataaaattga aaattttaaa ttttttagtc agtatgcaga
      781 ttttaaagat ttgaaaaatt ataaaaatgg aagaatatct atcaatgaaa atgttccgta
      841 ttatgaagca gagtacaaaa ggaataatag tgatggaaat gtaaaaaaac ttagagaaaa
      901 gtacccaatt acaaccaagc agtctccaat attaaaactg catatagacg gagatattaa
      961 aggtagctca gttggatata agcagataga gtacatgttt tctaaggaga aagaggatga
     1021 gacttttatg agtgattttt tgaacttcgg accatcacat agcaattagt aaataaaacc
     1081 aggggcataa actaatttaa aacaagagag aagtgaaaag atgggatatt ttaaaagagt
     1141 tttactttat ataattgtta tggttttgag tgtttttata ataggttgtg ataaatcaag
     1201 cgatacttca gaaaaatcaa aaggagattc aaaagaagca caaattaaaa agagttttgc
     1261 aaaaacgtta gacatgtatc ctactgaaaa tctagaagac ttttatgaca aagagggata
     1321 tcgagatgga aaatttaaaa aaggcgacaa aggtacttgg gttattagat ctgaaatgac
     1381 aacagaactg aaaaatgaaa atatggtatc taaaggtatg gtcatacgtt taaatagaaa
     1441 tagtagaaca tgcactggtg aatattttgt caggatagtt aaagaagaca gtgagggcaa
     1501 ggtatatagt gatgaacgaa aatatccagt gaaaatggaa aataataaaa tcattccatt
     1561 aaaaccaatc gatgatgaaa aagtaaaaaa agaaattgaa gaatttaaat tctttgtaca
     1621 atacgggaat ttcaaagaat tggaaaacta taaagacgga gaagtgacat ataacccaga
     1681 agcaccaata tactctgcac aatatcaatt gaaaaacagt gattacaatg tagaacaatt
     1741 acgtaagcga tataatatcc cgacgcaaaa agcgcctaaa ttattattga aaggctcagg
     1801 taatttaaaa ggttcatcag tcggatataa aaatattgaa tttacctttg ttgaaaacaa
     1861 gg
//
LOCUS       DBNENW010000259         1858 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNENW010000259 DBNENW010000000
VERSION     DBNENW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.263"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(55..>1858)
                     /locus_tag="AC1LW8_002713"
     CDS             complement(55..>1858)
                     /locus_tag="AC1LW8_002713"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIP4774458.1"
                     /translation="FHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVLLS
                     LTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGKFG
                     LGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQIT
                     VYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASELA
                     SRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVFTS
                     LHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELANKS
                     HADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQSI
                     VSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFVRD
                     KDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLDGLEGKKKIES
                     IMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGFIA
                     LRPSGTEPKIKLYVSLKCRNFDDVAQKINAMIFS"
BASE COUNT          616 a          324 c          297 g          621 t
ORIGIN      
        1 aagggctgat aagtataaaa tgaaatgtaa tatttcgatg tataaaattt ttaattagct
       61 aaaaatcatc gcattaattt tttgagctac atcatcaaaa ttccgacatt ttaacgacac
      121 atataattta attttaggtt cagtaccaga aggacgtaaa gcgataaatc cttcgtcaaa
      181 taagacacga ataacatttg atttaggaga attaatctgt gacgttgtat ctttatccaa
      241 atgataaacc tcgctagtta aataatcttc aattgctttc actttcagtc cttgaatctc
      301 ttgcggtgga tttgaacgga aatgtgtcat aattgattca atttttttct taccttctaa
      361 tccatctagc gtatgcgaga ataatgtatc ctcatgtcta ccaacagttt gataaatctg
      421 ttctaattca tctttcaatg ttttgccata taattttaac tcagaagcgt attttataat
      481 gagtggcaca atttgtacgg catctttatc acgtacaaaa ggctctgata gaaaaccgta
      541 actctcttca aaggcgaaaa tcatattttg atgatcgtcc agttgtctta tttcctgagc
      601 aataaattta aagcccgtaa gcacctcttt gtattcaaca ttattatagc gtgctaatga
      661 ctttgttaac tcactactta caatagattg aatcattaag cgatgacgta attgagacgt
      721 ttgttgaata cgataattaa gtaacaacgc accaatttgg ttgccattaa aatatgtgat
      781 atgaccatgt gcatcacgtt cagcaatgcc aagacgatcc gcatcagggt cagttgatat
      841 taataagtct gcatgacttt tatttgcaag ttcgactgct tgatcaaaag cgcgatgatc
      901 ttcaggatta gcactttgaa ctgagctgaa attcggatca ggtttacatt gtgcttctac
      961 aagattaaat tgattgaaat tcaaagattg taataactca ggtacaatcg gaacacttgt
     1021 accatgtaaa ctagtgaaca caacttgtaa atcagattta ggaatatagc caatcatatt
     1081 ttgaatgtgt ttcatatagt catcagttac agatttcgga aaaggcttga tataagatgt
     1141 attttgttta gaaataggta tatcaatctg taatggatcg ccaacttctt cgatataacg
     1201 acttgcaagc tcagatgcat cagtcgataa ttgcgcacca tcagaaccat atactttgat
     1261 gccgttatag tctttcggat tatgacttgc tgtaatcata atgccagcag tagtattaag
     1321 attgcgcacc gcgaacgata attccggtgt cgttttatat gtatcaggta aataaactgt
     1381 tatttgatgg tttgctaata cattagcaat tatttgggcg aattcagttg aaagatgtct
     1441 aatatcataa tgaatgacta ttgttggact gtttgtttgg gcatttaaat aacgcgctaa
     1501 acctaatgcc aatttttcaa tagtaaactt attaagtcga ccttcaccaa gaccgaattt
     1561 tccgcgtata cccgcagtac caaatgttaa tttactttca aaaccttctc gctgttcaat
     1621 gtcagattgc tgctcataaa aatgtttaac taaactatca ttagctcgtt ctatccataa
     1681 ttctttatcc attgttgcta aacatccttt caaaatctca gttagactta ataaaacatg
     1741 aaaactaaag cccttacatt tatgtaatga attataaaga aatacgcccc aaaagtaaaa
     1801 aaacacagcc ccaagacaat acttttcaca agtattatat aatagatgtg tatgaaaa
//
LOCUS       DBNENW010000260         1706 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNENW010000260 DBNENW010000000
VERSION     DBNENW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.264"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            86..625
                     /locus_tag="AC1LW8_002714"
     CDS             86..625
                     /locus_tag="AC1LW8_002714"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIP4774459.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            637..>1706
                     /locus_tag="AC1LW8_002715"
     CDS             637..>1706
                     /locus_tag="AC1LW8_002715"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIP4774460.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIAL"
BASE COUNT          618 a          197 c          291 g          600 t
ORIGIN      
        1 gaattgtcgc ataattcatc atgacaacat aatgacatgt attgttatta acgatataat
       61 agaattgaat aaagtgggtg ataatgtgac taaaaatgag attagaaaat acattttaca
      121 taaaatgaag aattttaata aagctgaaaa gcgaaaagca gacacatggt taagaaatca
      181 attttttgca actgaagaat acaaagaagc aaacgcaatt gcgctagttc tttcttttaa
      241 tcatgaagta gatacttttt ctattattga acaagcctta atggatcata aacgtatttt
      301 tgtaccgaaa atggattatt taaatcatca aatgactttt aaagaaatat ttaatctcaa
      361 agatattgat gtcgataata aggggattta ctatccaact tcaaaaggtg aaacaacgaa
      421 taacctagat ttaattgttg ttcctggtgt tggatttcaa gacgatggat atagaattgg
      481 gtatggtggt ggctattacg acaggttttt agctaattat cagacaaaga caataagctt
      541 attatacgat tttcaaataa catcatttga accggaatca ttcgatcaac cagtcgataa
      601 attgattata tatcaatcag catagtggag gactaaatga acatagacaa acaattttgg
      661 aaaacaatat attattggat taggtacttg aattttgata ttgtcagtag ggaaaaagat
      721 gaccaagaaa tttggttagc ccataaaaga aaaaaacaag tagtcatttt taaacaacat
      781 atcaagtcta cccaagaaat acgttttgac aaagcgaaag tgcttgaaca caaagatgaa
      841 atagcaaatt ttatttcttt cgaaccacaa agttttgaat tttattattt tacagaatcg
      901 gaattttcag aagaacaatt aaatgaagtt tcgccaatta gaattaaatt caatgttata
      961 agacacacaa aagatttgat aaagcatatg ccgaatatat ttttggctag acttatttca
     1021 gaagataatg ataaaaagac atatatgttc tataaacgca aagtattaac cgataacttt
     1081 ttagataaat atatgcagaa attttcaccg gcaacataca caataatatt tgtaaatgtc
     1141 ttaatatggt tatgtatgat tttatattta aataattttt cggatgtaaa attattagat
     1201 gttggcgggt tagtgcattt taatgtcgta catggtgaat ggtatcgaat tgttacatcg
     1261 atgtttttac attttagttt tgaacatata cttatgaata tgctttcatt atttattttt
     1321 ggtaaaatag tcgaagcaat tattggttca tggcggatgt taactgtata ctttattgca
     1381 gggttgtttg gaaactttgt atcactatca tttaatacga ctacaatttc agttggggct
     1441 agtggtgcta tatttggtct gattggatca atttttgcga tgatgtatgt ttcaaaaaca
     1501 tttaacaaaa aaatgttagg acagttatta attgcattag tgatattagt tggtgtttct
     1561 ctgtttatgt caaatataaa tattgtggcg catattggag gattcattgg tggtttatta
     1621 ataactttaa ttggctatta ctataaagtg aatcgtaata ttttttggat tttactaatt
     1681 ggtatgcttg ttatatttat tgcact
//
LOCUS       DBNENW010000261         1703 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNENW010000261 DBNENW010000000
VERSION     DBNENW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.265"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..1276)
                     /gene="cap8I"
                     /locus_tag="AC1LW8_002716"
     CDS             complement(<1..1276)
                     /gene="cap8I"
                     /locus_tag="AC1LW8_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HIP4774461.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQAYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVVLPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSILFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFLDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITS"
     gene            complement(1269..>1703)
                     /gene="cap8H"
                     /locus_tag="AC1LW8_002717"
     CDS             complement(1269..>1703)
                     /gene="cap8H"
                     /locus_tag="AC1LW8_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HIP4774462.1"
                     /translation="YFGTGSEKLEVFARENFINNITFSGGFDLKETPKYLNEIDILNN
                     LFGNQNIALDTALSIRMYYALFLNKPIITTDDTFTATEANKFGLGFSINPENLKGIGD
                     ELMDWYNNLDVMDINHKREAYRNDVIENNKQFYQEIGRIFNE"
BASE COUNT          654 a          286 c          202 g          561 t
ORIGIN      
        1 tactcgtaat aagtctcata taaatatatg taccaataaa tatcacttct aacgaatgac
       61 tcttccgaat aatatactca ctcagaaatg caaaaaagat atttgcaatt aaattcaaat
      121 agaagaataa gggtccaaaa taaataattc catatccagc actcgaaatt agatgaccag
      181 tcaattgttt actaccatat atcaattggt taaaaagttg gctagtaatc aactgctttt
      241 tatctaaaaa gaaattaaac ccaaaaacag ctctggtatt atcaaataaa tattgtttga
      301 acgaaccatt atagtaattc aaatagtcta tagaagctgc aatattgcta ggtccataaa
      361 aatatgactg caacgtgtca actattttaa gattacttac acttgtacta tttaacgcct
      421 cgctatatga actataatta aacacataca attctttata tagtgtcatc aatgctaata
      481 cgaaaatacc tacagatata ataatgatat tgatacgtct tttatgctta gaaaacaaga
      541 ttgacaccac tgttaaaaca gcaaccattg tataaagttg ataacttctt ctttcaccaa
      601 caattaaact aatatttaaa attcctatag ctagcggtaa tacaacataa ataattttag
      661 gattctcttt atacttttta tatgaagcat atgcgattat taagaataat aacgctaagg
      721 caagttgaaa tagcattaca aaaagaacat ttaaaccact tgttgcttcg gttcctcttc
      781 ccattgcatc tgttttaata attaaaaatg atatgttttt gcgcacttcg ggaaaagcta
      841 caaaaatccc acaaataaca agaccaaaca caatgtaata aattgatgat ccagaaagtg
      901 taaagctatt tcttgatacc acttcatgtt tgaataattt agatagtatc aatataaata
      961 gcgagcctat gataaactcc acactcatat aaataaccgc ttgattaaaa taagcttgtc
     1021 ctaatggtaa attatctata gttgaatcct ggaaaaacac tatcatagca ggtataaata
     1081 ctaatctaag aaaacctaaa acatcgtata caattttcga aaatttaaaa ttcggattat
     1141 tcgagaagct tataaagagc tttcctactc cataacttat aggtgctaaa aataataaag
     1201 gtttatcacc taacataagg caaggaaaca ttaaaatggc aataacatat gaagtgacat
     1261 tatatatctt attcattaaa tatcctgcct atctcttgat agaactgttt attattttca
     1321 attacatcgt ttctataagc ttctctttta tgattaatgt ccattacatc caaattgtta
     1381 taccaatcca tcaattcatc accaatacct tttaaatttt ctggattaat actaaaaccc
     1441 agtccgaatt tattagcttc tgtagcggtg aatgtatcgt ctgttgtaat aataggtttg
     1501 tttaaaaaca acgcataata cattcttatg gataaagcag tatctaaagc aatattttga
     1561 ttaccaaaca aattattaag tatatcaatt tcatttaaat atttcggtgt ttctttcaag
     1621 tcaaagccgc cagaaaatgt aatattatta ataaaatttt ctcgagcaaa gacttctaat
     1681 ttttccgacc ctgttccaaa ata
//
LOCUS       DBNENW010000262         1696 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNENW010000262 DBNENW010000000
VERSION     DBNENW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.266"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(53..1534)
                     /gene="cls"
                     /locus_tag="AC1LW8_002718"
     CDS             complement(53..1534)
                     /gene="cls"
                     /locus_tag="AC1LW8_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIP4774463.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGKVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLQDIKNAKEYIHLEY
                     YTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATYSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEMVSVGTANMDFRSFELNFEVNAFVYDEKLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            complement(1591..>1696)
                     /locus_tag="AC1LW8_002719"
     CDS             complement(1591..>1696)
                     /locus_tag="AC1LW8_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774464.1"
                     /translation="NLKLVNITIKEIEERTFPNNEEEFNAILTLSVNK"
BASE COUNT          575 a          317 c          216 g          588 t
ORIGIN      
        1 attttgggca aaataaaaag agcctctata atcgagactc cttacaaata aattataaaa
       61 ttggcgaaac taatttagct aacgattctt taaacttaac agacagcggt ctattggcat
      121 atgattcttt ggttagttgt tttgattttg taatatcatg ttcataagcc acccttaaat
      181 ctttagcaag cttttcatca tatacaaaag cattgacttc gaaattcaat tcaaaacttc
      241 taaagtccat attagcagtt ccgactgaaa ccatttcatc atctattaaa cacattttag
      301 aatgtataaa tccattttca tacgtgtaaa tttttacgcc actagatagt aaatctgaag
      361 catttgaata tgtcgcccaa taaactaaag gatgatctgg cttacatgga atcattagat
      421 gaacatctac ccctgattta gctgcaattt taatggcatt tatatatgag ttatccggaa
      481 tgaaataagg cgattgcaaa tataccgact tcttagcgct cataatcatt tttgtataac
      541 catattcaat ttgatgccag tcactagccg gaccacttgc agctatttga attggtgaat
      601 tgcccaatgg tccgttcttt ttagggaaat acttaacatc atattcaaac tgtggacggt
      661 gcgcttgcga attccagtct aaaataaatc gcaactgcag tgcatcaacc gcatcccctt
      721 gtatacgtaa atgcgtatct ctccaatacc ctagtttacc taatcctaga tattcatcac
      781 caatgttaaa tcctccgaca taacctagtt gaccatcgat tacgatgatt tttctatgat
      841 ttctgttatt cattctgaaa ttcaataacg gtaatttaga agcaaaaaat gcttcaactt
      901 ctccacctaa cgatttaaaa tgatcaaaat tagccatctt aactttcttt gaaccaacgt
      961 catcatataa aattttcact tctagacctt gtttcaattt ctcttctaac gcttttaaaa
     1021 ttctttggcc caagccatct agagcgaatg tataatactc taaatggatg tattctttag
     1081 catttttaat atcttgtaaa acttgatcat ataaatcatt tccatcaatg aaatgatcaa
     1141 ctttattatt ttcagttaaa aaaccatctt gatccatcaa aagcatacgt actaaatcat
     1201 gatgtttttg aacttgtttg ttatcagtac cataattacc tttatcaaag ctttctattt
     1261 gttgttttaa aagtccatcg aaatccgtta acaccttacc attgttttta ttcaatttac
     1321 gtgccgaaac ggttctacca aaaaacaagt aaagaataaa accaatcaat ggtaagacaa
     1381 aaagtacaaa tagccatgcc caagttgaac tcgctgtacg tctatttctt tctaaaaaga
     1441 taataataaa agccaatact aaattaatga tgaatccaat ggctaaaata atggtaaaaa
     1501 gtgttcctaa atcatttgaa aatgtatatc gcatagtctt ttctcctata aagaaaggca
     1561 caaaaaacat tttgcacctt tcacatcata ttatttattc acagataaag ttaaaattgc
     1621 attgaattct tcttcattat ttgggaatgt tctttcttct atttctttaa tagtaatatt
     1681 tactaatttt aaattt
//
LOCUS       DBNENW010000263         1678 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNENW010000263 DBNENW010000000
VERSION     DBNENW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.267"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            68..535
                     /locus_tag="AC1LW8_002720"
     CDS             68..535
                     /locus_tag="AC1LW8_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HIP4774465.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            659..>1678
                     /gene="sbcD"
                     /locus_tag="AC1LW8_002721"
     CDS             659..>1678
                     /gene="sbcD"
                     /locus_tag="AC1LW8_002721"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HIP4774466.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSI"
BASE COUNT          604 a          221 c          303 g          550 t
ORIGIN      
        1 gactgagaaa tgctataatt cattttgtat gatttacaga gagtttattt aacgagaagg
       61 tgtctgcgtg ctctatttaa tattttcaat cattgtagct ttatttatgg gaactatagt
      121 tatagttatt cgtatgaaag ctcaaaatta tccggtaaat gagaaaaaaa tagttttgcc
      181 accgtttttt atggcgaccg gtgcattgat gtacgtcgtt ccatatttta ggctaacagg
      241 atcggaaatg ctagaagcct ttataattgg tttgcttttt tctacagttc taatttggac
      301 ttctcgattt gaagtcaaag gtacagaaat ttatatgaaa cgatctaaag catttccagt
      361 tattttgatt tcattactta tcattcgtac tgtgatgaaa atattcatta gtaatgaaat
      421 agatcctgga gaattaggcg gcatgttctt tttattagca ttctgtatga ttgttccttg
      481 gagagcagca atgctatata aatacaaaaa actaaagaaa acattaatca attaattact
      541 tttaaaacca cttgtgatcg acttctaaat cagtcaatga gtggttttaa ttttacttga
      601 aaaggtgaaa tgattatatt aaaataaaag cgaacaaatg ttctatatgg aggcagtaat
      661 gaaaattata catacagcag actggcactt agggaaaata ttaaatggca aacagctttt
      721 agaagatcaa gcgtatattt tagatatgtt cgtagaaaaa atgaaagaag aagaacctga
      781 tatcattgtg atagctggag atttatatga cacaacatat ccaagtaaag atgcaatcat
      841 gttattagaa caagcgattg gaaagttaaa tttagaactg cgtataccaa taattattat
      901 tagtggaaat cacgatggta aagagaggtt aaactatggg gcgagttggt ttgaacataa
      961 tcagttattt ataagaacag attttacatc gattaattca ccaatagaga taaatggggt
     1021 taatttttat acactccctt atgctactgt gagcgaaatg aaacactact ttgaagatga
     1081 caccattgaa acacatcaac agggaattac gcgctgtatt gaaacaatag caccggaaat
     1141 tgatgaagat gccgtcaata ttttaattag tcatctgact gttcaaggtg gaaagacatc
     1201 tgattctgaa agaccattaa ctattggaac ggttgaatca gttcagaaag gtgtttttga
     1261 tatatttgat tatgtcatgc taggtcactt gcatcatcca tttagtatag aagacgacaa
     1321 aattaaatat agtggctcct tattgcagta ttcattttcg gaagcgggtc aagctaaagg
     1381 gtatagacgt gtaacaatta atgatggcat tattaacgat gtatttattc ctcttaagcc
     1441 acttagacaa ttggaaatta tctcaggcga atataatgat gttattaatg aaaaagttca
     1501 tgtgaaaaat aaagataatt atttacattt taaacttaaa aatatgtctc atattactga
     1561 tccaatgatg agtttaaaac aaatttatcc taatacttta gcgctgacga atgaaacttt
     1621 taattacaat gaagaaaata atgctataga aataagtgaa aaagatgaca tgtcaatt
//
LOCUS       DBNENW010000264         1674 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNENW010000264 DBNENW010000000
VERSION     DBNENW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.268"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..583)
                     /locus_tag="AC1LW8_002722"
     CDS             complement(<1..583)
                     /locus_tag="AC1LW8_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqG family protein"
                     /protein_id="HIP4774467.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMVLKEMK
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDNLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAYLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFA"
     gene            complement(762..1142)
                     /gene="gcvH"
                     /locus_tag="AC1LW8_002723"
     CDS             complement(762..1142)
                     /gene="gcvH"
                     /locus_tag="AC1LW8_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIP4774468.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(1300..1656)
                     /locus_tag="AC1LW8_002724"
     CDS             complement(1300..1656)
                     /locus_tag="AC1LW8_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIP4774469.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
BASE COUNT          512 a          319 c          196 g          647 t
ORIGIN      
        1 gcgcgaagga tggattgact ttattccaca atgcaagcac gtcctcatcg tacttataat
       61 cagtacatgt aatagtttgt ttcgacaatc taaattctat agaaaatgct ccactgaacg
      121 ggatatcctc attatcagca tctaatgact tcaaatacgc ttgaatattt tcgagtgata
      181 aatctgtaat tggctctttt atataaacaa gccttgagtt tatattgttt ccctttaaat
      241 tttcagacat accatacatt tgatcttctg aactaatcca aaactgcaat atcccttctt
      301 ttacaggaaa taattcttgt tgttctggaa gattatccaa attaatctgt gctaacaata
      361 ccattggtac attattttca tctaatggta tatctttaaa agattttaaa aatggcattc
      421 caccaatctt actctggttt aatgtttctt ctttcatctc ttttagaacc atttcaatac
      481 atggtagtac tggtaaatta ccgttttcta cagattctac gaaacggtta tatgcttttt
      541 gctccttttt agaaagatga ctaacgtctt gaatgtttag catattaact tcccccaatt
      601 tttattttta aaatagctaa attcattatt ttcatcattc ttgaataata aaatgtagca
      661 atactccaaa taatagtacc taataaattt tctttcatat tatttactta ctcagactaa
      721 aaataaactc ctcaatctta gattaaggag ttacacggtg attattcacc aatcatttct
      781 gaatattttt cagctgttaa taaagcttca atctgacttt catcactaat ttctactttt
      841 accatccatg ctttttcgta tggagattca tttacaaatt cgggactatc ttctagttct
      901 tcgttgactt caactacttt accagagatt ggtgcatata attctgatac agttttaact
      961 gattctacgc taccaaacgt atccccttca ttaatttcat catctgtttc tggtaattca
     1021 acgaaaacaa tatcacctaa ctcgctttgt gcgtattctg tgattccaat tgttgctaca
     1081 ttaccttcaa ctttaaccca ttcatgctct tttgaatatt tcaattcatt tggtactgcc
     1141 aagagaatcc cctcctaatt aatctttaca tcattaatga tgccataagg ctagatgcta
     1201 ttcaactata tttataaaaa ttaacaattg tatagcttat ttcattttac atgtaatgaa
     1261 tctaaatatc gagatattta gtaaacgttt acattatact tacgctaacc aagtctcttt
     1321 atattgatct tctttaaatc ctaacgttat cttatcgccc attactgcta gaggacgctt
     1381 tactaacata ccatcagatg ataacaactc taacttttca tcatctgata aagtttgtaa
     1441 tttatttttc aaatcaagct cacgatattt cgcgccgtgt gtattaaaca atttattaat
     1501 ttctacgcct gtatttgcaa ttattgtttt aaattcattt attgtaggtg tatgttgaac
     1561 gatatcaatt ggttcataac ttacgccata ttcatctaaa aactttgctg cctttttaca
     1621 agttgtacaa ttcttatatt ggtaaaattt aatcatacga ctgcaacctc cttt
//
LOCUS       DBNENW010000265         1665 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNENW010000265 DBNENW010000000
VERSION     DBNENW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.269"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..113
                     /locus_tag="AC1LW8_002725"
     CDS             <1..113
                     /locus_tag="AC1LW8_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HIP4774470.1"
                     /translation="NNYTIDLSKRLPSTDANRYVKKPQNAKIEVILEKSN"
     gene            221..937
                     /locus_tag="AC1LW8_002726"
     CDS             221..937
                     /locus_tag="AC1LW8_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HIP4774471.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KGRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(1383..1607)
                     /locus_tag="AC1LW8_002727"
     CDS             complement(1383..1607)
                     /locus_tag="AC1LW8_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774472.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(1564..>1665)
                     /locus_tag="AC1LW8_002728"
     CDS             complement(1564..>1665)
                     /locus_tag="AC1LW8_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774473.1"
                     /translation="IFLGNEIIHTLTVLITTLYIVNSRKGIKNDRVE"
BASE COUNT          593 a          250 c          249 g          573 t
ORIGIN      
        1 acaataacta cacaatagat ttaagtaaaa ggttgccatc aactgatgca aatagatatg
       61 ttaaaaaacc tcaaaatgca aaaattgaag ttatcctcga aaaatcaaac taacaataat
      121 aatggagtta ataaaaataa tcgcaaatac tatattgact tcgctcacat ttaaatttct
      181 tattcctcgt atcatgattc ctctgaaagg agatgttcta atgagtaaga acatcacgaa
      241 aaatataatt ttaacgacaa cattattact attaggtact gtattacctc aaaatcaaaa
      301 accagtattt agtttttact ctgaagctaa agcttatagc attggtcaag atgaaactaa
      361 catcaatgaa ttaattaaat attacacaca gcctcatttt tcattttcaa ataaatggct
      421 atatcaatat gataatggaa acatttatgt tgaacttaag agatattcat ggtcagcaca
      481 tatatcttta tggggcgctg aaagttgggg aaatattaat cagttaaaag gtcgttacgt
      541 agatgtgttt ggactaaaag acaaagatac tgatcagtta tggtggtctt atagagagac
      601 atttacaggt ggcgttacac cagccgcaaa accttctgat aaaacttata atctttttgt
      661 gcaatacaaa gataaactac aaacgattat tggtgcgcat aaaatatacc aaggcaataa
      721 accagtatta acattgaaag aaatcgattt ccgtgcacga gaagcgttaa taaaaaataa
      781 aatattatat aacgaaaatc gtaataaagg taagcttaag atcaccggtg gcggtaataa
      841 ctacactatt gatttaagca aaagattaca ttcagatcta gcaaatgttt atgttaaaaa
      901 tcctaataaa ataactgttg acgtcctctt tgattagtat atgaaggtga cttatacttc
      961 atgcacttta attccaaatc agattattta aatgataatt tttaaagtgt atgatgtata
     1021 taataggtaa aattttctat atatttaaat ggaattggga gtaggaatgt gacagaaata
     1081 gtattttata aaatttattt cgttgtcact ccccaacttg cattgtctgt agaatttctt
     1141 tttgaaattc tctgtgttgg ggcccatccc caacttgcac attattgtaa gctgactttt
     1201 cgtcagcttc tgtgttgggg ccccgcctat aattgaaaaa tgcttgttac atgggcattt
     1261 tcattcggtc aactactact aatataatat tgtagagcct aagacattga tttattatgt
     1321 cttaggctct attccttcat ttaatgattc aattattata gcaatacttt attgtcccat
     1381 gattagtgtt cttttaatga gacatagtaa ctataaagtt taataatcgt tctaaatcta
     1441 gcatcttcta gtttggtttt cccatttctt aaatcttgta ctgtttgata tggaactcct
     1501 gaatttttcg aaattttata gcctgattca gactcaaaca aagattgaag agagtttata
     1561 atattattca actctgtcat ttttaatccc ctttcttgaa ttaacaatat ataatgttgt
     1621 tattaaaaca gtcagtgtat ggatgatttc atttcctaaa aatat
//
LOCUS       DBNENW010000266         1645 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.270, whole genome shotgun sequence.
ACCESSION   DBNENW010000266 DBNENW010000000
VERSION     DBNENW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.270"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            52..1608
                     /locus_tag="AC1LW8_002729"
     CDS             52..1608
                     /locus_tag="AC1LW8_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIP4774474.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     LLITQVLIYS"
BASE COUNT          500 a          250 c          333 g          562 t
ORIGIN      
        1 taaaatcttt aaggtagaaa tgctaggtta aatgtcgaag gagatgaaac cgtggaaaat
       61 acaattaatg aaagtgaaaa gaaaaaacga tttaaattaa aaatgccagg tgcatttatg
      121 attttattca ttttaacggt tgttgcagtt atagcaacat gggttattcc tgctggtgca
      181 tattctaaac tttcttacga accttcatcc caagaactaa agatagttaa ccctcataac
      241 caagtgaaaa aggttccggg tacgcaacag gaactagaca aaatgggagt taaaattaag
      301 attgaacaat ttaaatcagg tgcaattaat aagccggtat caattccgaa tacttatgaa
      361 agattaaagc aacatccagc tggaccagaa caaataacaa gtagcatggt tgaaggtacg
      421 atagaagcgg tcgatatcat ggtattcatt cttgtactag ggggacttat tggcgtagtt
      481 caagccagtg gttcttttga atcgggattg ttagctttaa cgaagaaaac aaaagggcat
      541 gaatttatgc taattgtgtt tgtatcaata ctaatgatta tcggcgggac gttatgtggt
      601 attgaagaag aagctgtagc attctatccg attttagtcc ctatatttat agcgttagga
      661 tacgattcta tcgtttcagt tggtgccata ttccttgcca gctctgtcgg tagtacattt
      721 tcaactatta acccgttctc ggttgtaatt gcctctaatg ccgctggtac aacttttacg
      781 gatggcttgt attggagaat aggtgcttgt attgtcggtg cgatttttgt tattagttat
      841 ttatattggt actgtaaaaa aattaaaaac gatcctaaag cgtcatattc ttatgaagac
      901 aaagatgctt ttgaacagca atggtctgta ttaaaagatg acgatagtgc ccattttact
      961 ttgcgtaaga agataatcct tacattattt gtactaccat ttccaattat ggtatgggga
     1021 gttatgacgc aaggttggtg gttcccagtt atggcttcag catttttaat atttacaatt
     1081 ataataatgt ttattgctgg gacaggtaaa tctggattgg gagaaaaagg aactgtagat
     1141 gcatttgtca atggcgcatc aagtttagta ggtgtatctt taattattgg tttagctcga
     1201 ggtattaatt tagtgttgaa tgaaggtatg atttcagata caatcttaca cttttcatca
     1261 tctttagttc aacatatgag tggaccatta tttatcatcg tattactatt tattttcttc
     1321 tgtttaggtt ttatcgtgcc atcatcttct ggattagcgg tattatcaat gcctatcttt
     1381 gcaccactag ctgatacagt aggtatacca agattcgtca tcgttacgac atatcaattt
     1441 ggtcaatatg cgatgttatt cttagcgccg actggacttg ttatggccac actacaaatg
     1501 ttaaacatgc gatattcaca ttggttccga tttgtatggc cggtagttgc ttttgtattg
     1561 attttcggtg gcggactact aattacgcaa gtactaattt attcataatt tgaaatgcta
     1621 tattataaaa atactaaatg ggttt
//
LOCUS       DBNENW010000267         1634 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.271, whole genome shotgun sequence.
ACCESSION   DBNENW010000267 DBNENW010000000
VERSION     DBNENW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.271"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..556
                     /locus_tag="AC1LW8_002730"
     CDS             <1..556
                     /locus_tag="AC1LW8_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HIP4774475.1"
                     /translation="FLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEKMTD
                     GQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSIDFKG
                     LKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPVSGK
                     VGEGVTSGGENTLTKRFINKITID"
     gene            complement(1030..>1634)
                     /locus_tag="AC1LW8_002731"
     CDS             complement(1030..>1634)
                     /locus_tag="AC1LW8_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BsaG family lantibiotic immunity ABC transporter
                     permease subunit"
                     /protein_id="HIP4774476.1"
                     /translation="INWYLNVNLWNGRQISLFTASFNAITSLLISINVYQVINFEENI
                     GHFNHILGKANRLNWLNASMIFTYTITAICILLASINLLWHSHDMKITLMFIGVSLFF
                     NVIILLLLFIFSIFIKDVMAIVVGVLMFIFNVYFGLEVLGDHSWFYLPITYATRYVYM
                     FIEGSIPVTLTLAIYIIITLLLAVLLFKGFNKWSGRTIKD"
BASE COUNT          628 a          275 c          211 g          520 t
ORIGIN      
        1 atttttaggt aaaacattag tagcaagtgc tttagtatta acaacattag gaacaggctt
       61 acattcttca tacttaggat tagatacaaa taaagttgtt aaaacggcta aagcagaaga
      121 aaaaatgaca gatggtcaat tatggaaaaa agttaaagat tcattacatg attctgatat
      181 tattttaagt aacgagtacg aaacaatcaa cgttacttat ttattaagca atggatattc
      241 tagcagcgtt tctgctcctg gaaatgatga tggaggccat ctaacacaat ctatagactt
      301 taaaggatta aaacaaattg atttaactaa ggaaaatgtc tacgatgact ttaacaaaaa
      361 gctagatgct aaaaatacat ggaattctct aaccgagaag ttaaaaggtc ttggtttact
      421 acaaaatggc caaaaagtat ctatttattc ttcagatagc tcttctccag tctcgggtaa
      481 agtgggagaa ggagtaacta gtggtggcga aaatactcta actaaacgct tcatcaacaa
      541 aatcacaatt gattaaacct taaactcaaa tttccttaat tcataaaaca ttaataacta
      601 accctttctt acaaaccacc tagcccataa tatattaaaa cgtcattttt atttcaatta
      661 gcggaaaata aaaaaaccat tcctcattaa ctttggcaag ttggaatggt taaaaaaata
      721 ttttagaagc cagcgtttgg cttgcttaga cgacatgttc gcgcatgtcg tcttattttt
      781 acatttatat aataacacgc aattcaataa aatgtctaat caataaacct caattacatt
      841 caaattcaaa aacttttcac ctaatattca tcgcttaaat aaacattacg tttaattaaa
      901 cagacattgt caatcgcgtc taatacccct gctgtccgaa aacaaaatta ttttgtattc
      961 atatttaact gttctctttc ctcccttttc aacgcaacta aatttctttt accgttagag
     1021 ccccaatctt taatctttaa ttgttctacc gctccattta ttaaatcctt taaagagtaa
     1081 aactgctaat agcaacgtga taataatata gattgccaat gttaatgtaa ctggtatact
     1141 cccttcgata aacatataaa cgtaacgtgt agcatatgtg attggtaaat agaaccacga
     1201 atgatctcca agcacttcta atccaaaata aacgttaaaa ataaacatta aaactccgac
     1261 aacaatagcc attacatctt taatgaaaat actaaaaata aaaagtagca gtaatataat
     1321 tacattgaaa aacaatgata cgcctataaa cataagtgtt attttcatat catgtgaatg
     1381 ccacaataaa ttaattgatg ctaatagaat acatatggct gtaatagtat aagtaaaaat
     1441 cattgatgca tttaaccaat ttagcctatt agcttttcct aaaatatgat taaagtgacc
     1501 aatattttct tcaaaattga taacttgata gacgtttata gaaattaata gcgatgtaat
     1561 tgcattaaaa ctcgctgtaa acaaacttat ttgtcgacca ttccataaat ttacgtttaa
     1621 ataccaattt ataa
//
LOCUS       DBNENW010000268         1627 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.272, whole genome shotgun sequence.
ACCESSION   DBNENW010000268 DBNENW010000000
VERSION     DBNENW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.272"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(291..1382)
                     /locus_tag="AC1LW8_002732"
     CDS             complement(291..1382)
                     /locus_tag="AC1LW8_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HIP4774477.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          521 a          278 c          221 g          607 t
ORIGIN      
        1 acatttcagc aacaaatgtg tgaatctatt atattttaca gataattcat agttataatt
       61 ttaattactg ctatcagtca taatctttaa taattgtgaa attgaacaag tgtcgaagta
      121 ttataacaat cgttaagtgc agtatacctt tatttctata caaaaaataa tagccactct
      181 aaaagtaata tactcttaga atggctgtaa gtttaaaatt gtcgattcaa agataatatt
      241 ctacttcaac atgacatatt agttaagtag tctatagttt ccttggaata ttattttaac
      301 tttttagcgt tatataaatc agctaaaggc tttaattcat cataaaatgc ttggaattca
      361 tctaaatcca tttgttgacc cgcatcacta agtgcaacag atggatctgg atgcacctca
      421 gccataactc catcagcacc aactgctaat gctgctttcg cagttggtaa catgatatct
      481 ttacgacctg tactatgcgt tacatctacc atgactggta agtgtgtacc ttgttttaaa
      541 attggtactg ctgaaatatc taaagtgtta cgtgtcgcct tttcataagt tcggattcca
      601 cgttcacata aaataatgtt ttgattacct tgtgaagcaa tgtattcagc tgcataaaca
      661 aactcttcga ttgtagcaga taaaccacgt tttaatagaa taggcttttt cgtacggcca
      721 gcttctttta ataactcgaa gttttgcata ttacgtgcac caatttggaa tacgtctaaa
      781 tactcatcag ccacttcaaa atcatttgga tttacgattt cgctgacaac atttaaatca
      841 tatttatctt taatctgttt aagtatttta agtccttcaa cacctaggcc ttggaaatca
      901 tatggtgatg tacgtggttt aaatgcaccg ccacgaataa atttttcacc tttagcatgt
      961 aagtttttag caacagcttc aacttgttca aatgattcaa ctgaacatgg cccaaataca
     1021 aatgatttat tgccgtctcc aataatgccc ccattatcaa atgttacaat cgtatcttca
     1081 ggtttcaact tacgtgatac atataaatgt ttttcatttt cagatttttg taaatctgta
     1141 gaggctttga aaatttcttt aaataattgc ttaatagtat tatcgttgaa tggtcctttg
     1201 ttactatcga ttaagtcgtt aagcatttct ttttcacgtt gtggatcata gatacgtgta
     1261 ccttgtttta atttttcttc cccaattttt tgtgctagtt caccacgttt agataataag
     1321 tctaaaattt gatgattcag tgatacaatc tcacttctgt atgattctaa tttattactc
     1381 atccttactc acctctacta attatttcaa gtatattcca ctttaaagca ataaaatata
     1441 atgttaaaaa atattctaac aatatcatat gtatagttca accctaaatt gacagaaaat
     1501 aatcaatttt ctaattatat gattcgagac tgacatttcg attttctcag tcattattgg
     1561 aaagttatat tctatattga tatttattag caaatgttaa tcaattaaat aagtgtcatt
     1621 attatag
//
LOCUS       DBNENW010000269         1618 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.273, whole genome shotgun sequence.
ACCESSION   DBNENW010000269 DBNENW010000000
VERSION     DBNENW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.273"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..867
                     /gene="pmtC"
                     /locus_tag="AC1LW8_002733"
     CDS             <1..867
                     /gene="pmtC"
                     /locus_tag="AC1LW8_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HIP4774478.1"
                     /translation="LEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMKVM
                     NGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDKII
                     DAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLTTI
                     KSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVDHK
                     DFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSSLR
                     DTYFNINQRGDK"
     gene            867..1607
                     /gene="pmtD"
                     /locus_tag="AC1LW8_002734"
     CDS             867..1607
                     /gene="pmtD"
                     /locus_tag="AC1LW8_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HIP4774479.1"
                     /translation="MRILNLVKYDLYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
BASE COUNT          581 a          188 c          264 g          585 t
ORIGIN      
        1 ttagaacata ttacaaaaaa atacggctca aatgtcgttt taaatgatat tgattttgac
       61 tttggcgata gtagaattgt cggattaata ggaaaaaacg gtgttggtaa aacaaccgtt
      121 atgaaagtaa tgaatggtaa tattattaaa tttgatggaa aagtagacat tgataatgca
      181 gataatatcg gttttttaat tgagcatcct aaattatatg ataataaatc aggattgtat
      241 aacttgaaat tatttgcaca agtattaggt aagggttttg ataaagcata cacagacaaa
      301 attatagatg catttggtat gagaccttat attaaaaaga aagttaagaa atattcaatg
      361 gggatgaagc aaaagttagc aattgcagta tctttaatga ataaacctaa atttttaatc
      421 ttggatgagc ctacaaatgg tatggatcca gatggctcaa ttgatgtgct gactacaatt
      481 aagtctctag taaatgaact tgatatgaga attctaatat caagtcataa gttagaagat
      541 attgaattaa tttgtgatag agctgtattt ttaagagacg gacattttgt tcaagatgta
      601 aacatggagg aaggtgttgc atctgacaca acgatagtta ctgttgatca taaagacttt
      661 gatagaactg aaaaatatct tgcagagcat ttccaattac aaaatgtcga caaagcagac
      721 ggacatttaa tgattaatgc acaaaaaaat tatcaagtta tactaaaagc attatctgaa
      781 ttagatattt atccgaaata tattgaaaca cgtaaaagtt cattgcgtga tacgtacttc
      841 aatataaatc aaagaggtga taaataatga gaattttaaa tttagttaag tatgatcttt
      901 atagtatttt taaaagtcct ttaacatatt tagcgatact agtcgtatct agtttgattg
      961 caactcaaag tatacttatg gcaaattcta tggataaccc gaaacatatt attgtctatg
     1021 gatctgtatt tgctgcagca aaatggttat tgttaataat tggattaatg tttgttgtta
     1081 agacaattac gcgtgatttt tcacaaggta caattcaact atatatgagt aaagttaaaa
     1141 cacgcgttgg atacattatt tcgaaaacaa tttcaattat tttaatttca atattatttg
     1201 cattaattca ttatgtgatt ttgattgttg tgcaggcatc tagtaatgga aaaaatttgg
     1261 cgttttctaa atatgtagat aatttatggt tcttcctaat ctttttacta ttctttggct
     1321 tgtttttatt cttaatcaca cttgcatcac aaaaaacagc aatgatattt tcattaggtg
     1381 tatttttagt acttattgta ccgtttatta aaccttttat tacatttatc ccaagatacg
     1441 gtgaaaaagt tttagatgct tttgattata tcccttttgc ttacttaact gataaaatga
     1501 ttagctctaa ctttgatttt agcaattggc aatgggtaat ttcattaggt tctatagtaa
     1561 tattcttcat tttgaatatc ttatatgtcg ctaaaaaaga catttaataa aaataatt
//
LOCUS       DBNENW010000270         1616 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.274, whole genome shotgun sequence.
ACCESSION   DBNENW010000270 DBNENW010000000
VERSION     DBNENW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.274"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            52..>1616
                     /gene="eap"
                     /locus_tag="AC1LW8_002735"
                     /gene_synonym="map"
     CDS             52..>1616
                     /gene="eap"
                     /locus_tag="AC1LW8_002735"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HIP4774480.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLK
                     ISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIF
                     TPNLFSAKDIKKIDIDVKTGSKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISYKY
                     LNDKVKSVLKSERG"
BASE COUNT          713 a          219 c          233 g          451 t
ORIGIN      
        1 ggctataata aagttgcaat cacgaattac ataaaaaagg agagataatt tatgaaattt
       61 aagtcattga ttacaacaac attagcatta ggcgttatag catcaacagg agcaaacttt
      121 aatactaacg aagcatctgc cgcagctaag ccattagata aatcatcaag tacattacac
      181 catggacatt ctaacatcca gattccatat acaattactg tgaacggcac aagccaaaac
      241 attttatcaa gcttaacatt taataagaat caaaatatta gttataaaga tatagagaat
      301 aaagttaaat cagttttata ctttaataga ggtattagtg atatcgattt aagactttca
      361 aagcaagcgg aatatacggt tcattttaaa aatggaacaa aaagagttat cgatttgaaa
      421 tcaggtatct acacagctga cttaatcaat acaagtgaca ttaaagctat cagtgttaac
      481 gtagatacta aaaagcaacc taaagataaa gctaaagcaa atgttcaagt gccatataca
      541 atcacagtga acggcacaag ccaaaacatt ttatcaaacc taacatttaa taagaatcaa
      601 aatattagtt acaaagattt agagggtaaa gttaaatcag ttttagaatc aaatagaggt
      661 attactgatg ttgatttaag actttcgaag caagcgaaat atacagttaa ttttaaaaat
      721 ggaacgaaga aagttataga tttgaaatca ggtatttaca cagcgaattt aatcaattca
      781 agtgatatta aaagtatcaa tattaacgta gatacaaaaa aacatatcga aaataaagct
      841 aaaagaaact atcaagttcc atattcaatt aatctaaatg gtacatctac aaacatttta
      901 tcgaatcttt cattttcaaa taaaccttgg acaaattaca aaaatttaac tagtcaaata
      961 aaatcagtac tgaagcatga tagaggtatt agtgaacaag atttaaaata tgctaagaaa
     1021 gcttattata ctgtttattt taaaaatggt ggtaaaagaa tcttacagtt aaattcaaaa
     1081 aattacacag caaacttagt tcatgcgaaa gatgttaaga gaattgaaat tactgttaaa
     1141 acaggaacta aagcgaaagc agacagatat gtaccataca caattgcagt aaatggcaca
     1201 tcaacaccaa ttttatcaaa actaaaaatt tcgaataaac aattaattag ttacaaatat
     1261 ttaaacgaca aagtgaaatc tgtattaaaa agtgaaagag gtatcagtga tcttgactta
     1321 aaatttgcga aacaagcaaa atatacagta tatttcaaaa atggaaagaa acaagtagtg
     1381 aatttaaaat cagacatctt tacacctaat ttatttagtg ccaaagatat taaaaagata
     1441 gatattgatg ttaaaacagg gtcaaaagcg aaagcggata gctatgtacc atatacaatt
     1501 gcagtaaatg gcacatcaac accaatttta tcaaaactaa aaatttcgaa taaacaatta
     1561 attagttaca aatatttaaa tgacaaagtg aaatctgtat taaaaagtga aagagg
//
LOCUS       DBNENW010000271         1613 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.275, whole genome shotgun sequence.
ACCESSION   DBNENW010000271 DBNENW010000000
VERSION     DBNENW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.275"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(94..432)
                     /locus_tag="AC1LW8_002736"
                     /pseudo
     CDS             complement(94..432)
                     /locus_tag="AC1LW8_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1080..1571)
                     /gene="sak"
                     /locus_tag="AC1LW8_002737"
     CDS             complement(1080..1571)
                     /gene="sak"
                     /locus_tag="AC1LW8_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HIP4774481.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVI
                     EKK"
BASE COUNT          528 a          298 c          236 g          551 t
ORIGIN      
        1 aacacattgc tgtgtctaaa atgattatag cataatttca aactattcta taataaatta
       61 attaacttca tatgaaactt tacacacttt ttttcaaagt gcgctaaaat taccaaaact
      121 acttattcta ttacctgcct tgtctacctc tcctgtcgct atataacaac gttgtccatt
      181 attagcaata taagtaatcc atctatagcc attaatgcaa tatgcgccgt catatttgat
      241 cgttgcgtta ttgggtaata cacctgtaat tcttgaatta gttgaatagc catccctcac
      301 gttattacct ttaacattgg caactgtgta ataaccagtc tcttttttat acggtacatt
      361 gtttttatcg agtgtataac ctgctggcac tggtgaattc ttttcatttt tagctggtgt
      421 tttaacatta ctgatacctg atacacactt ccaataaaaa taaccacacc atttaagatg
      481 cggtgtagcg actgtaatat ttctatgttg ttgagatata tgtatcgagt gaagggcaaa
      541 aaggatatca attgcaggat aagtgttagg ttactaggcc acttaacagg ctatatagtt
      601 cactcctact atatacactt aattataaca taaaaagcac cccgtaaact attatacggg
      661 aatgctaaag tcatatatac tacgggggag tagtatgaaa actatgctct ctatcataag
      721 aaaaaaacac ccagtgacat gcttgggtga acaaggatag atgtaaatag ttgatgcatg
      781 tgtaacacat catagcaaaa aactagcccg aaagctagct ataacataca atctaaaaag
      841 acgtcctttg aatacgtcta gaaagattat aacataaaaa aataggcaag taccgaagta
      901 cctgcctaaa aaaggattat ccactttttc atcctaactg atttctcccc ataagtcacc
      961 taatatctga ttaggtgggg aagaaccatt cgtgcatgaa tgagaatttg atgaaagata
     1021 attttcacta cacatattca atcaagacat tgctttctat aataaacaac tattttgttt
     1081 tatttctttt ctataacaac ctttgtaatt aagttgaatc cagggttttt aatatgctct
     1141 gataaatctg ggacaacaaa acctttttct gttataggga aagacttcgt ttcttctttt
     1201 ttcttattct tatcataata agtgacttcg atctttgcgc ttggatctaa ttcaactact
     1261 ctaaactctt tatatgctgt cgcatctaat gcccattcga catagtattc aattttttct
     1321 tttgtaagtg tagtcccagg tttaatagga aactcgacat aatgagggga tagcaattca
     1381 tttcctttac catcaactcc agtcacattt accatcaaat acgggcctgt tggttcaaaa
     1441 taactcgcgt catcgccttt tttatatttt cctttgtcga atgaacttga tgcacttacc
     1501 tcattagtaa ttgaagaaaa tgagaataat aacaataaaa cagttaaaaa taataaactt
     1561 cttttgagca tggcgcttcc tccaaatata atatatttgt ttttatttat ctc
//
LOCUS       DBNENW010000272         1597 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.276, whole genome shotgun sequence.
ACCESSION   DBNENW010000272 DBNENW010000000
VERSION     DBNENW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.276"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            15..1181
                     /gene="scpA"
                     /locus_tag="AC1LW8_002738"
     CDS             15..1181
                     /gene="scpA"
                     /locus_tag="AC1LW8_002738"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HIP4774482.1"
                     /translation="MKRNFPKLIALSLILSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSIPTEVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKVTKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YVNKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
     gene            1212..1538
                     /locus_tag="AC1LW8_002739"
     CDS             1212..1538
                     /locus_tag="AC1LW8_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HIP4774483.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKQKS"
BASE COUNT          631 a          230 c          243 g          493 t
ORIGIN      
        1 aaaggagttt ttatatgaaa agaaactttc caaaattaat agctttaagt ttaatacttt
       61 ctttaagtgt tacccctatt gcaaacgctg agagcaattc aaatatcaaa gcaaaagata
      121 agaagcatgt gcaagttaat gtcgaggaca agagtattcc tactgaagta cgtaatttag
      181 cgcaaaaaga ctatctttct tatgttactt cacttgataa aatctataat aaagagaaag
      241 cctcgtatac acttggtgaa ccttttaaaa tttataaatt caacaaaaag agtgatggta
      301 attattattt tccagttttg aacactgaag gtaatatcga ttatatagtt acaatttcac
      361 caaaagtcac taagtattct agttcatctt ctaaatacac aatcaatgtt tctccatttt
      421 tatctaaagt cttaaatcaa tataaagatc aacaaattac tattttgaca aactcaaaag
      481 gttactatgt ggttactcaa aatcataaag ctaaactagt actaaaaaca ccacgtctag
      541 aagataaaaa gttaaaaaag actgagagta tacctactgg caataatgta acgcaactaa
      601 agcaaaaagc ttctgtcact atgccaacta gtcaatttaa aagtaataat tatacttaca
      661 acgagcaata tgtaaataaa cttgagaatt tcaagataag agaaactcaa ggtaacaatg
      721 gttggtgtgc aggctatacg atgtctgcat tactaaatgc gacttataat actaacaaat
      781 atcatgcaga agctgtgatg agatttttac acccaaactt acaaggacaa cgattccaat
      841 ttactggttt aacgccaaga gagatgattt attttggaca aactcaggga agaagtccac
      901 aacttcttaa tagaatgact acatataatg aagttgacaa tcttactaaa aataataaag
      961 gaattgctgt tttaggtagt agagtagaat caagaaatgg tatgcacgca gggcatgcca
     1021 tggcagttgt tgggaacgct aaattagata atggacaaga ggttataatt atatggaatc
     1081 catgggataa tggttttatg actcaagatg ctaaaaacaa tgttattcca gtatccaatg
     1141 gcgaccatta tcaatggtat tcatctattt atggttatta aaatttgtat tttagaaagg
     1201 taaataatat tatggaacaa tttgaattat ttagtattga taaattcaaa tgtaattcag
     1261 aggctaagta ttatcttaat attattgagg gagaatggca tcctcaagat ttaaatgata
     1321 gtcctttaaa atttattctc agtacctcag acgattctga ttacatttgc aaatatataa
     1381 atacagaaca caaacaactc acattatata ataaaaataa tagctcaatt gttattgaaa
     1441 tatttatacc aaatgataat aaaatactac taacaattat gaacacagaa gctttgggaa
     1501 cttctcctag aatgactttc attaagcaaa agtcataaga ctatctagat atgattaagt
     1561 aaactattaa tcttattaaa ggtctctagg aagaaaa
//
LOCUS       DBNENW010000273         1594 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.277, whole genome shotgun sequence.
ACCESSION   DBNENW010000273 DBNENW010000000
VERSION     DBNENW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.277"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            251..976
                     /locus_tag="AC1LW8_002740"
     CDS             251..976
                     /locus_tag="AC1LW8_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HIP4774484.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSLSNKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPKNAQIEVILEKSN"
     gene            1071..>1594
                     /locus_tag="AC1LW8_002741"
     CDS             1071..>1594
                     /locus_tag="AC1LW8_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HIP4774485.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKE"
BASE COUNT          618 a          245 c          230 g          501 t
ORIGIN      
        1 tagaaaattc aattatcaaa caatttaatg caattgatta gagaataatt gtaacgtgtc
       61 gtttttaata tataactccc gcctacttta ttaagtactg tttctgtcca aaacttaaaa
      121 atgataagtt ttgcttaaat aacactacta actgtttaag tttatttaac atagttttag
      181 cttttattta attccgaatc ggtgtaatag cttatatact ttgggtaatt cacgcaaagg
      241 agattttcat atgaaaaaga acatcatgaa taaattagtt ttatcaacag cattgttact
      301 tttaggaact acatcaacac aacttcctaa aacaccaatc agtttttcat ctgaagcaaa
      361 agcctataat atcagtgaaa acgagactaa tatcaatgag ttaataaagt attatacaca
      421 gcctcattta tcactatcaa ataaatggtt atggcaaaag cccaatggta gcattcatgc
      481 aacattgcaa acgtgggttt ggtatagtca tattcaagtg tttggatccg agagttgggg
      541 aaacattaat cagttaagaa ataaatacgt tgatatattt ggaactaaag atgaggacac
      601 agttgaaggt tactggactt atgatgaaac atttactggt ggtgttacgc cagcagctac
      661 ttcatctgat aagccttata gactattttt aaaatatagt gataaacaac aaactatcat
      721 cggtggacat gaattttaca aaggaaataa accagtatta actttaaaag aattagattt
      781 ccgtattcgt caaacattaa taaaaaataa aaagttatat aacggagaat ttaataaagg
      841 tcaaattaag ataactgctg atggaaataa ttacacgatt gatttaagta aaaagttaaa
      901 attaactgac acaaaccgtt atgttaaaaa tcctaaaaat gcacaaattg aagtcatact
      961 cgaaaaatct aactaaccta ttaccttttg taaatgcgga taatttcaat tatctaatta
     1021 accccttttt ataattaaac attccaacaa tactcaaagg agaaattcga atgaacaata
     1081 acatcacgaa aaaaattatt ttatcaacaa cattgttact attaggtaca gcatctacac
     1141 aatttcctaa tacacctatc aattcttcat ctgaagcgaa agcttattat ataaatcaaa
     1201 acgaaactaa cgttaatgag ttaactaaat attactcgca aaaatattta accttctcta
     1261 acagtacgtt atggcaaaaa gataacggta cgattcatgc aacgttgtta cagttttctt
     1321 ggtatagtca tattcaagtt tatggacctg aaagttgggg caatatcaac caattaagaa
     1381 ataaaagcgt tgatattttt ggcataaaag accaagaaac cattgattct tttgcattat
     1441 ctcaagaaac gtttactggt ggtgttactc ctgcagcaac atctaacgat aaacactata
     1501 aactgaatgt gacatataaa gataaagcag aaacgtttac tggcggattt ccagtttatg
     1561 aaggcaataa gcctgtttta actttaaaag aatt
//
LOCUS       DBNENW010000274         1585 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.278, whole genome shotgun sequence.
ACCESSION   DBNENW010000274 DBNENW010000000
VERSION     DBNENW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.278"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..234
                     /locus_tag="AC1LW8_002742"
     CDS             <1..234
                     /locus_tag="AC1LW8_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774486.1"
                     /translation="DILLIVSIFINLVYSLNSYLNLRYNINEHIALAAVEQSTLEKQK
                     ERLHFQDNIKKAHKKVYREFVSKQNKDKKGKDK"
     gene            231..686
                     /locus_tag="AC1LW8_002743"
     CDS             231..686
                     /locus_tag="AC1LW8_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774487.1"
                     /translation="MMFITGIVISLVVILIASYFSFVLTTLIISEDFSDRVTLKVAFI
                     LPIILISLSIRACLENLRSNKQLAKTAFKMATTQYPSVVTMFIGMVKDHQAQVHCFGE
                     STYTYNQNKENLILKKDINKSWLNSLKNLKSLIKSEGFKKNFLNNLAEA"
     gene            931..1116
                     /locus_tag="AC1LW8_002744"
     CDS             931..1116
                     /locus_tag="AC1LW8_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774488.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            1207..>1585
                     /locus_tag="AC1LW8_002745"
     CDS             1207..>1585
                     /locus_tag="AC1LW8_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HIP4774489.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSE"
BASE COUNT          610 a          221 c          262 g          492 t
ORIGIN      
        1 gatatattat taatagtcag tatttttatt aatttagtgt attctttgaa ttcttatttg
       61 aacttacgct acaatataaa tgaacatatt gctttagctg cagtagagca aagtacgtta
      121 gaaaaacaaa aagaaagatt gcactttcaa gataatatta aaaaagcaca taaaaaagta
      181 tatcgtgaat ttgttagtaa acaaaataaa gataaaaagg ggaaggataa atgatgttta
      241 taactggaat agtgatatcg ttagttgtaa tcttgatagc ttcatatttt tcctttgttt
      301 taacaacatt aattataagc gaagatttta gtgaccgagt tactttaaaa gtagcattta
      361 tcttaccaat aatattaata tccctatcca ttcgtgcatg tttagaaaac ctaaggagta
      421 ataaacaact tgctaaaaca gcatttaaaa tggctactac acaatatcct tcagtggtaa
      481 caatgttcat aggtatggtt aaagaccatc aagcacaagt ccactgtttt ggtgaatcta
      541 cctataccta taaccaaaat aaagaaaacc taatattaaa aaaagacata aataagtcat
      601 ggttaaattc attaaagaat ttgaagtcac ttattaaatc agaaggattt aaaaagaatt
      661 ttttaaataa cttagctgaa gcttaagttc tcgtattacc ttcgatgatt atgaattttg
      721 ctcagcatgt aaaaccacac cacctattaa tttaggagtg tggttatttt tgtgtttttt
      781 cagggcaaaa aaagggcgga ttatttaaat aagggcaaac acttgtggaa aatttaaaag
      841 gttaaaaata ataaagaact tggtataaca agggttttat acatttgcgt acaacgacga
      901 aatgtcaatt taccatcaca ttatgacgat atgtttattt taaacacaca agctcatgcg
      961 cgtcttgatc aaatggcaca acagtttgaa gttgtttgta atggcttgaa cgaaaatgaa
     1021 ggacaagcaa ttcaaacgat ggatcaatct gcctctctaa tacggtcaaa cttaattcaa
     1081 gttaaagaac aattagaaaa actagctgta tactaattaa tttattaaat gctacttgtt
     1141 ttcattgaga ataagtagtt ttttcaaaca taaaagtttt acaaacacat aaatgggtga
     1201 tgagctatgt ttaaaagaac taaactaatc ttaatagcaa cgattctact atcaggatgt
     1261 tcaactacca ataacgaatc caacaatgaa acaaaatctg tgccagaaga aatggaagct
     1321 tcaaaatatg taggtcaagg cttccaacca cctgcagaaa aagatgcggt tgaatttgcg
     1381 aaaaagcata aagataaaat tgctaaacgt ggcgaacaat tttttatgga taactttgga
     1441 ctaaaagtta aagctacaaa tgttgtaggt agtggcgacg gtgtagaagt attcgtgcat
     1501 tgtgatgacc acgacatcgt atttaatgca agtattccat ttgacaaatc aattattgac
     1561 agtgatagct cattaagaag tgagg
//
LOCUS       DBNENW010000275         1584 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.279, whole genome shotgun sequence.
ACCESSION   DBNENW010000275 DBNENW010000000
VERSION     DBNENW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.279"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            78..1568
                     /locus_tag="AC1LW8_002746"
     CDS             78..1568
                     /locus_tag="AC1LW8_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIP4774490.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          656 a          144 c          312 g          472 t
ORIGIN      
        1 atcgcgtgaa atactatggt agacggtttg agtaaattaa tggagtattt taatatttat
       61 ggtttggagg aattgcgttg catagagaaa gtaaatatat agagtataag aaatcacgaa
      121 aaggattatc taatgatatt tggtctacgt atagtgcttt tgcaaatact gaaggtggta
      181 ctatatattt aggaattgaa gaaaaaaaga tcgaggacaa aaaagtcttt gtttcagttg
      241 gtgttgaaga tccagagaaa atgattgaag atttttggaa tgcactatat ggaagaagta
      301 aagttagtca aaatatttta tcaaataaag atgttaaaat tgttaatatt gaaaataaag
      361 cgtgcattga aattcatgta ccagaagcgc cttattcgaa gaaaccgata tatgtagata
      421 ataaaaaaga tttagtatat aaaagagttg atgatgctga tagaattgcg actgaagaag
      481 agtataaatt catgattgta aattctcaag acgatataga tacagaatta ttagataact
      541 atgacatgtc tgatttaaat cacgaatcta tcgaaaatta taggaaactt ctattaaaaa
      601 atactaatga tgagagatat gcgaatatga gccaactgga tttaatgata gatttaggag
      661 catatagaaa agatagaagt tcgaaagaca aacagtataa aatgactaca gcatgtttat
      721 tattctttgg taagtataat gcgattagtg atagattccc aggatttcaa ttagattatt
      781 ttaagaaaac aaattaccta gatactgatt ggaaagatag aatatcaagt ggagatttag
      841 gtaatgaaga tttaaacgtg tatagttttt ttgaaaaagt attgataaaa ttaactgata
      901 acattgagga atcatttagc ctaaatgatg gtttgactag acaaaattat gcaagagatt
      961 taaaagtagc aattcgcgaa gcactggtta atacattaat gcatgcgtat tatgatacta
     1021 agcaaagtat taaaatagtt aattgtgaag attttataga gttttataat ccgggtaata
     1081 tgagaataaa taaagaagat tttattcatg gagggcattc aaaggacaga aatagtatat
     1141 tatcgacgct tttcagaaga gtaggatatt cagaaaaagc tggatctgga ggaccaagga
     1201 tattcgatgt agttaataga cataagctta aaacgcctga aatagaatta acggacatgg
     1261 acactaatgt agtactttgg aaacaagatt taatgaagga gtttgaaaaa tatcctgagt
     1321 tagacaaaaa agtaataaag tatattattg actatggatc aataagtaag ggtgaagcct
     1381 taaaaatgga aaatatgaca gaatatcagt ttagaaatat tttaaaaaaa ctaaaagatg
     1441 ataacttgat aaaaaaagaa ggtgaaggtc cggctactaa atatgtgtta atagaatcaa
     1501 aagaagctga tatattgcga actaaaaaag taattaaaag tttagagtct ttctttagga
     1561 ataaataaaa aaacaagaga tagg
//
LOCUS       DBNENW010000276         1570 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.280, whole genome shotgun sequence.
ACCESSION   DBNENW010000276 DBNENW010000000
VERSION     DBNENW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.280"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(338..1561)
                     /locus_tag="AC1LW8_002747"
     CDS             complement(338..1561)
                     /locus_tag="AC1LW8_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIP4774491.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIKYLKEK"
BASE COUNT          475 a          282 c          238 g          575 t
ORIGIN      
        1 aattatttga tagaaatcag cttttttcaa atgtatttga taatttacag ctcgttgagc
       61 tgctgttttc tagggattta tgtcccattc cttttacgag aaggttttta cgagttttat
      121 gtaactatta gaagctatct ttttatatga ccattagtca atatatgtat gacaaatcag
      181 cacaataatt tcagttttac atcacttaat caatgaaaat gtgcttgttg atatttgaat
      241 atttacattc aaacaatatt catcgttatt tctatcaact taaaattcgc aataaaaaac
      301 tgccagcatg cgaatagggc agctgacagc atcgttctta tttctctttt aaatatttaa
      361 tggaagcctc tttaaaaata tcaatatatt tcagatacat atctttttca atatattcat
      421 cgatttgatg tgccattaat ggattacctg gtccaaaaat ggctaaatca acattgtcct
      481 tattatctcc taagaaacta gaggcatctg ttgcacctac aagcgctgaa acaaatattt
      541 cgtcttgttc tacataacta gaagctacat ctttaatcgt agtaattaat ttgctatttt
      601 tatcgcttgt tacaggtcgg tgattgcttg gaatatcgag tgaaagctta ttgctatcca
      661 catcattaat gatattttgg aaaaacgatt ctataaagtc gttatcatac tcaggaactg
      721 gtcttacgtt aaattcaagt gaagcttcat ctggtacaga gttaaattgt ttgccgccat
      781 ttataatcga acatacagct gtaagaccag atgcataatt tgcatcctct tcagaaattt
      841 cttttccaat caatgatttg aacataggcg caacatctaa ttcatgttta gtatcttgtt
      901 gtttaagctc tgaatatttt tctttaaata gattataaaa ttcaagcagt gtatcaattg
      961 cattgtcacc aataaatgga actgagctat ggacagcttt accagttgca gttactttac
     1021 atgacataga ccccttatgt gcataataaa ttccagatcc agttggttca gcaataatta
     1081 agccatcgac atcgtctaaa tagcctttat cagctaataa tttggcacct tcttgttctt
     1141 tctcttcgcc agcagtagct agtaatctta tcgttccttg aggcaattga ttttgttctt
     1201 ttaattcaat gagtgtgatg accaaagcca tcagaccgcc tttcatatcc gttgtgcctc
     1261 gaccgtataa tttgccagct ttttctgtga gttgaaaagg gggataagtc caattatctt
     1321 gatttcctgc atcaacaaca tccatatgac cactcaatgc gagtataggt gagccgttac
     1381 cgatttctgc aacgatattg gcgcggtgtt cattaacttt caaaatttca gatttaatat
     1441 cgtacttgtc gaataaatct tttaaataat tacaaacgtc tatttcatta ttattttcag
     1501 tttgtagttc aacaatatct gctagtaatt gaattttttc tttttcacta aaagttgtca
     1561 ttaagctcac
//
LOCUS       DBNENW010000277         1567 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.281, whole genome shotgun sequence.
ACCESSION   DBNENW010000277 DBNENW010000000
VERSION     DBNENW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.281"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            139..1178
                     /locus_tag="AC1LW8_002748"
                     /pseudo
     CDS             139..1178
                     /locus_tag="AC1LW8_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558176.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          629 a          206 c          243 g          489 t
ORIGIN      
        1 ttttattgag gtgaacaatg aatcgtctac acttaattgg acacattcta tgagagtaga
       61 ttttgttaat ttaagaaagt aggcgatttt attatgacta gagaaagaag atcatttagt
      121 tcaaatttta agttataaat ggttagatta tataaaaatg gtaagcctag gaatgaaatt
      181 atacgcgagt atgatttaac accttccgcg ttagggaaat ggataaagca acatcaaaac
      241 acaggttgat tcaatcatca agatagctta acagatgaag aaaaagagct gattaaatta
      301 ggcaatgaag ttcaacatat aaaaatggag aacgatattt taaagcaagc agcgctgatc
      361 ataggacgaa aatagaagtc attcaaaaga atgcacatca atattcagtt ccggcaatgt
      421 gtaaagtcct aaaaatacca agaagtacct attatgattc tataaaaaga aaagataata
      481 aaatcactaa agatgattca aacgtagaac gtgctgtcat aaatattttt aattctaata
      541 gaaaagtctt tggtgcaaga cgaaagatag ctcgtatcat gaaaaaacat aatctagttt
      601 ctgtttatac gaaagctaaa tacaaaaatt atccaaaaga aactaatgaa aaacgaatta
      661 aaaatcattt gaatcgcgct tttaatagag aacaaccaat ggagacatta gtaagtgatt
      721 tgacatatgt aaaagtcgca ggaacatggc attacatatg tttatttata gatctattta
      781 atagagaaat tgttggttac agtgcaggta aaaataagga cgcaaattta gtatcaaaag
      841 caatcagcag aataaatcat aatcttgaac aaatcaacct gttccatacc gatagaggca
      901 aagactttga taatcatttg atagatgaag tcctagaatc ctttaaaatc aaatgttcat
      961 taagtaccaa aagttgtcct tatgataacg cagttgcgga agcaacgatg aaagcaatga
     1021 gatctgaatt tgtaaaacaa atgtaatttg aaaacttaga acagttagaa acagaattat
     1081 ttgattatgt aaattggtat aacaatttta gactacattc ttcattacag tatttgacgc
     1141 cagtggcatt taaaaatcta catatgaaaa atgtctaaaa aaatgttgac ataccaggtt
     1201 atagaatacc aattggaagt atttatgagt ttcatcgaag atgttaaact gtccagctta
     1261 atttgacaat ctaaatgtaa aatcattaat aattaaacaa aacttaaaat atttaacttt
     1321 ttgtgattat tccacattat cttatctgct ctttatcacc ataaaaatag aaaaaacaag
     1381 attcctaaag aatataggaa tcttgtttca gactgtggac aaactgattt tttatcagtt
     1441 agcttattta gaaagtttta tttaaattac agtttctatt tttattagat cacaatttta
     1501 ttttagctct tgttcaagta atcatttttc gccaaaaact ttatactgaa tagcttctac
     1561 attaaat
//
LOCUS       DBNENW010000278         1546 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.282, whole genome shotgun sequence.
ACCESSION   DBNENW010000278 DBNENW010000000
VERSION     DBNENW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.282"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            120..1397
                     /locus_tag="AC1LW8_002749"
     CDS             120..1397
                     /locus_tag="AC1LW8_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774492.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
BASE COUNT          471 a          215 c          326 g          534 t
ORIGIN      
        1 tataaaaaat aactttggta caattttgtg acaaagtagg ggtggacatg ccagatgtcc
       61 gaaagtttta tcgttctagg gtaagttttt aaaaattaat aagggaagta ggaatagtta
      121 tgaaggaaaa acgaactaac gtaaggtgga tgttcgcgct tgcgttcttc tttattgggg
      181 ttattgcgta tatggataga gcaaacattt catatatcgc taaacaaatg atggatgatt
      241 tagggatgac caaaccacaa tttggcttat tggcatcatt cttctctcta ggttatgcat
      301 taatgcaagt accatcgggg atgttggctg aaaaattcgg tccacgtaag atgattacaa
      361 ttgcattagt ttggtggagt gcatttacaa tcttaacggg tatgattaag aaccacggtt
      421 taatttattt agtgagattc ttatttggtg taggtgaggc gccaatgtac ccttctaatg
      481 ctgtgtttaa ctcattttgg ttctctaaaa atgaaaaagg tagagcatca agtgcattat
      541 tagcaggatc atatttcgga cctgtattag caccaatagt tacaattgct attgttaacg
      601 catttaactg gcaagcagta ttttacattt ttggtgcagt aggtatttta atggctgtat
      661 tatgggcgat tattgccaaa gacttacctg agcaacatag aatggttaat gaagcggaga
      721 aacgtttcat tatggaaaat cgtgatatcg tagctactga aaagtcatca ccaccatgga
      781 atgatttctt taaacgtttt agcttctatg caattgcaat tcaatacttt gttgtacaat
      841 ttatcattac attattctta atttggttac cgacgtattt aacagaagta ttccacgtta
      901 actttaaaga aatgagcatt agttcattac cttggttatt aatgttcttc ttaatcttat
      961 cagcaggtgc aatttctgac cgtgtattag gattaggtcg ttcaaaattc gtagctagag
     1021 gtgtaattgc aatcgcagga tttattgtgt ttgcagtttc aattatcttt gctgtacgta
     1081 caggaaattt atatgtaagt attttctggt tatcactagg tcttggtggt atcggtattt
     1141 caatgggtat gagttgggct gcagcaactg acttaggacg taacttctct ggtacagtat
     1201 cagggtggat gaacttatgg ggtaatatag gtgcattaat cagtccgcta ttagcaggtc
     1261 tattcgtaga acatttaggt tggacaatga cattccaatt gttaatcgtt ccagcagtaa
     1321 tcgctgtgat tatgtggttc tatgtgaaac cagatcaacc tttaattgtt agtgatgata
     1381 aagcaataga aaaataattt aaacaagcag taagctttca cattgttggg gcttattgct
     1441 tttttacgtt gaaattgaaa ctttttaaaa cagatatggt ttaagatgaa cgggaagtta
     1501 ttaaaatgat atatgtaaag aaataaagtt ttaaaacatt agtcag
//
LOCUS       DBNENW010000279         1542 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.283, whole genome shotgun sequence.
ACCESSION   DBNENW010000279 DBNENW010000000
VERSION     DBNENW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.283"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            52..840
                     /locus_tag="AC1LW8_002750"
     CDS             52..840
                     /locus_tag="AC1LW8_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP4774493.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1090..1464)
                     /gene="sarA"
                     /locus_tag="AC1LW8_002751"
     CDS             complement(1090..1464)
                     /gene="sarA"
                     /locus_tag="AC1LW8_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="global transcriptional regulator SarA"
                     /protein_id="HIP4774494.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          535 a          209 c          245 g          553 t
ORIGIN      
        1 gcgtatatag aatgaaggtt gggtatatag tttattgagg gaggtgtcac aatgaataaa
       61 gtcacaatta atcctcaaat ccaattaact tatcaaattg aaggtaaagg ggatcctata
      121 atattacttc atggattgga tggtaattta gctggatttg aagatttgca acatcaacta
      181 gcatcatcat ataaagtact tacttacgat ttaagaggtc atggcaagtc ttctaaaagt
      241 gaatcatacg atttaaacga tcacgttgag gatttaaaaa ttctaatgga gaagttaaat
      301 attcatgagg cacatattct aggacatgat ttaggtgggg tagttgctaa gttatttaca
      361 gataaatatg cttatcgtgt aaaatcatta actaccattg catcgaagaa agatgactta
      421 atacacagct ttactcaatt gttaatacaa tatcaagatg atatagcggg ttttaataag
      481 tctgaagcgt atattctttt attttctaaa ttgtttagaa atcaagagaa gacgatgaaa
      541 tggtatcaaa aacaaagaat atatagcatt aagtctgagg atgatagtgc ggtggcaatt
      601 cgttcattaa ttttgcataa agatgaacct atgtatttaa aaaaacgtac atgtgtacct
      661 actttgttaa ttaatgggga acatgatcct ttgattaaag ataagaatca ttttaaattg
      721 gaagcgcatt ttttaaatgt tacgaaaaaa atcttcgaac attcaggaca tgcaccgcat
      781 attgaagaac cagaagcatt tatgaattat tatttaaatt ttttaaaaag cgtatcataa
      841 tatgtgatat ataaacctag ggcataaagt ctttaggcaa tgtgaaaaag ctgattacta
      901 ttcattattt gatagaaatc agcttttttt gaaatgtatt tgatatatac tgctcgttat
      961 gcggctatct tccttatatt aagtgccatt agtgcaaaac ctcttaacat ttacaaaaaa
     1021 agacataaaa aaaggaagtt taatagaatg tatcatctat caaacttcac caaattgcgc
     1081 taaacaaaat tatagttcaa tttcgttgtt tgcttcagtg attcgtttat ttactcgact
     1141 caataatgat tcgatttttt tacgttgttg tgcattaaca agaattaata cagttctttc
     1201 atcatgctca ttacgttttt tatcgaagta atcttcttga gataaaattt taactgcttt
     1261 aacaacttgt ggttgtttgt agtttaaatg attaataata tctttaagat agtattcttt
     1321 ctctttgttt tcgctgatgt atgtcaatac agcgaattct tcaaagctaa ttgaaaattc
     1381 ctttttaatt aaacttttta atttgtcagc ataagtgacc attgataaca actcaaagca
     1441 atcattgatt tttgtaattg ccatgtttaa aacctcccta tttgatgcat cttgctcgat
     1501 acatttgccc gataatatat tgatatctaa tctttattta tt
//
LOCUS       DBNENW010000280         1513 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.284, whole genome shotgun sequence.
ACCESSION   DBNENW010000280 DBNENW010000000
VERSION     DBNENW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.284"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..466
                     /gene="fmhC"
                     /locus_tag="AC1LW8_002752"
     CDS             <1..466
                     /gene="fmhC"
                     /locus_tag="AC1LW8_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HIP4774495.1"
                     /translation="EMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKTEELIF
                     EDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNF
                     YGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV"
     gene            639..1511
                     /gene="dprA"
                     /locus_tag="AC1LW8_002753"
     CDS             639..1511
                     /gene="dprA"
                     /locus_tag="AC1LW8_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIP4774496.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          583 a          193 c          233 g          504 t
ORIGIN      
        1 tgaaatgatg actgtgaaca gagcattaaa tgaaaatcca aattctaaaa aaaataaatc
       61 aaaattaaat cagttaaata tgcaattatc tagtattaat aatagaatta gtaaaaccga
      121 agaactaata tttgaagatg gacctgtttt ggatttagct gctgctttat ttatatgtac
      181 tgatgatgaa gtttattatc tatcaagtgg atcaaatccg aaatataatc agtatatggg
      241 tgcatatcat ctacaatggc atatgataaa atatgcaaaa tcacataata ttaataggta
      301 taatttttat ggaataacag gcgtctttag taatgaggcg gatgattttg gtgttcaaca
      361 atttaaaaag ggttttaatg cacatgttga agaattaatt ggtgatttca tcaaaccagt
      421 aagaccaatt ctatataaat ttgcaaaact tatttataag gtttaattat aaagtatgtt
      481 ggaaattgaa attttaaatt ctttccaaca tacttttcac tttttaagtc agtattttta
      541 ctccttaaaa taacattaca tcgacaatgt ttttgtatta gaaatgtaaa acaaaaatag
      601 aatatgattt tgtatgatta ccaaaaggag ttgatttatt gattaaacta tttttgctta
      661 agttatactg ggcacacttt tcgactaaac aaattcatca atttttaatg gcatatccta
      721 atgtaattaa agaggaggga agaaaaaaag atagttattt atgtgaatgg gtgaataggg
      781 aagaaaatgt tcatttatta cgtaaatact atgcttttat aaaacttgat cataacgata
      841 ttattaaaga actgcagaaa ttaaaagtaa gttacattac atatatggat tctgaatacc
      901 cagtgctatt aaaagaaata tatcaatttc cattacttct tttctataaa gggaacatca
      961 aattaataaa taatatgcat catttggcag tagtaggtgc aagagattct acaagttata
     1021 cccaacagtc tttagaattt ttattatcaa atgataaaag caaatattta acaattgttt
     1081 ccggccttgc tcaaggagct gatgcaatgg cacatcaaat agctttaaaa tacaatctcc
     1141 ctacaattgc agttttagcc tttggccatc aaacacatta tcccaaaagt acattagcat
     1201 taagaaataa aatagaagaa aaaggtttag ttatatccga atatccacca catacaccaa
     1261 ttgctaaata tagatttcct gagcgcaata gaattatcag cggtttgtca aaaggggttt
     1321 taattactga ggctaaggaa caaagtggca gtcacatcac gatagatttt gcattagagc
     1381 aaaatagaaa tgtttatgtt ttacctggat ctatgtttaa tcctatgaca aaaggtaatt
     1441 tattacgtat ccaagaaggt gctaaggtag tattaaacgc taatgatata tttgaagact
     1501 actatattta aaa
//
LOCUS       DBNENW010000281         1486 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.285, whole genome shotgun sequence.
ACCESSION   DBNENW010000281 DBNENW010000000
VERSION     DBNENW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.285"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            191..914
                     /locus_tag="AC1LW8_002754"
                     /pseudo
     CDS             191..914
                     /locus_tag="AC1LW8_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
     gene            complement(963..1436)
                     /locus_tag="AC1LW8_002755"
     CDS             complement(963..1436)
                     /locus_tag="AC1LW8_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIP4774497.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT          519 a          259 c          200 g          508 t
ORIGIN      
        1 aacgacttat acaaactcga agttatataa gtccccttat ggaatttcac actctagttt
       61 acataatata tattatagga agttatgtgt gtgtaacgca aaaggtaccc tacatcataa
      121 tcattatcta atatcgtcac ataacttact tatgctataa tcatggtatt atattgtttg
      181 gagtgatttg atgagatttg tctttgatat tgatggtacg ctttgttttg acggccgatt
      241 aattgaccag actattattg atacattgtt acaattacaa catgatggtc atgaacttat
      301 atttgcatca gcacacatta attggcgcaa atggtgctat gatttcacag caatcaaaga
      361 tttctgttat caaaccaatt catacagata catatcacca tatcttaaaa ataattcaaa
      421 agtatgagtt agattatatt attgatgatg attggaatta tgctgcacaa cttgacgctg
      481 agaacgcgat ttttgagcgt ttagatccac ataagctggc cagttgtatt gatgttgcaa
      541 atatcgacac gccaatcaaa gttattttat taaatataga cccggcacaa attacaacta
      601 tattagacga gctaaataaa taccatcaag aattggaaat gattcaccat tcaaatgagt
      661 ataacattga tataacagcg caaaatatta ataaatatac tgcattacaa tatatatttg
      721 atgcagatgt taaatatata gcatttggta atgaccacaa tgatattgtc atgttacaac
      781 atgctagtag tggctatatt ataggaccat cagaagcata cacacacgca atattgaaac
      841 ttgataaaat caaacacaaa cacatcaata ataatgcaca agctatttgc aaagtcttaa
      901 aatcatataa ataaaaaaca cccctatcaa atgataatca ttatcaatcg ataggggcta
      961 ttttaataaa attcgtcctc gaacatttct tcctcttcat ctaatccaaa taattctgcc
     1021 atttctccat gttcaattaa catgtttaaa tatgcatcgc ggagttcttc ttcactcata
     1081 tcattaatca tttctttaag actatcaatc cacatatttc tgcgtaattg atagtcttct
     1141 tcaacttcgt ttaacatcat tatatgttta tttgctgctt ctggactagc tgtaaagagt
     1201 aatgcaatca tatgtttaca tatcactcgt cttccatcag catgaggaca attacatttg
     1261 gattttctag gatgttccat atcaatataa caacgatata ctttgttgcc actgcccttt
     1321 acttcagcct catgctgcgt ttctgaaaat gattttaagt taatgacgca ttcactttga
     1381 taataattaa agcctctttc tatagaacga atacttgcaa tatcaagtaa tcccattaat
     1441 gatactcctt tttattatta tttttaaata aagaaaataa aataaa
//
LOCUS       DBNENW010000282         1480 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.286, whole genome shotgun sequence.
ACCESSION   DBNENW010000282 DBNENW010000000
VERSION     DBNENW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.286"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            506..>1480
                     /locus_tag="AC1LW8_002756"
     CDS             506..>1480
                     /locus_tag="AC1LW8_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIP4774498.1"
                     /translation="MGYRIETRTFEELEGKLKLPTFQRALVWSNNQKQEFFSTLKDGF
                     PFGSILLYEYENDNKYSLIDGLQRYSTMVDFMENPTNYIEFEDFTLKIAELYKGASES
                     TKAEVVNYAEDILLKIIEKYIDSTKENVKVNELAKKMVDKFPVLGETDIRDEITQIQS
                     DVITYFNNQLDISNVKIPCIFFEGEETELAEVFQRLNSGGKKLSKYQVFAAHWDRYEV
                     ELGDLKYSDEILKNVIERYERLNNDRGILIENFDPKEMETSRVINLSELCYGLGKVIS
                     DELQAFFKESTEDICNELGFQTMLIVFGIPTNKMNELPYKYQYMKNKNN"
BASE COUNT          565 a          149 c          268 g          498 t
ORIGIN      
        1 gtcagctaac gcctcttaac aatgcgaaac atagctggcc tttttttgta ataggaaaat
       61 ttatagttat ggctaaatca cttgaaattg acaaatctat aattaaatac gcacgtaagt
      121 aattttgatc gttcattgtt attgttttat gttttaggaa atgtgctatt gaaaggttta
      181 aagatgattg gaatttcttg gtttcttgac ttatatagtt atgaaagagg gcgttgtcct
      241 taatggcatt tctgagatca gaaatgctat taataaacat ttggaatata caaatataac
      301 tgaagaatgt atttaaaaac ttagaatgta tatctatcct taaatagttg tagtactatt
      361 gatctttaaa cataagatgt aaatggaaaa gatattgtat tttaacaaca tttcaattta
      421 taatatattt gtatgtcata tttctagcaa tttttatagt tttatagcta tttaattcgt
      481 atttgagata aaaggagagg tttggatggg atatagaata gaaacaagaa cgtttgaaga
      541 gttagaagga aaattaaagt taccaacttt tcaaagagca ctagtttggt ctaataatca
      601 aaagcaagaa tttttctcaa cgctgaaaga tggttttcct tttggttcta ttctgttata
      661 cgaatatgaa aatgataata aatacagtct tattgatggc ctgcaaaggt attctacaat
      721 ggtagatttc atggagaatc caacaaacta tattgaattt gaagatttta cattgaaaat
      781 tgctgaattg tataaagggg catcagagag tacaaaagca gaagtagtta attatgccga
      841 agatattctg ctgaaaatta ttgaaaaata tattgattct acaaaagaaa atgtaaaagt
      901 aaatgaacta gctaaaaaaa tggtggataa atttccagta ttaggtgaaa cagatattag
      961 agatgaaatc actcaaatac agtcagatgt tattacttat tttaataatc aactagatat
     1021 tagtaatgtc aagattccgt gtatcttctt tgaaggagaa gagactgaat tggcagaagt
     1081 ttttcaaagg ttaaacagtg gaggtaaaaa gttatcgaag tatcaagttt ttgctgcaca
     1141 ttgggacaga tatgaagttg aattgggaga tttaaagtat agtgatgaga tacttaaaaa
     1201 tgtaatagaa aggtacgaaa gattaaataa tgatagaggt atcttaattg aaaattttga
     1261 ccctaaagaa atggagacgt caagagtaat taatttatct gaattatgtt atggacttgg
     1321 taaagtgatt tctgatgaat tacaagcatt ttttaaagag tcaacagaag atatatgcaa
     1381 tgaattagga tttcaaacaa tgctaattgt atttggcatt cctacaaata agatgaatga
     1441 gttaccatat aaatatcaat atatgaagaa taaaaataat
//
LOCUS       DBNENW010000283         1465 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.287, whole genome shotgun sequence.
ACCESSION   DBNENW010000283 DBNENW010000000
VERSION     DBNENW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.287"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            170..1324
                     /locus_tag="AC1LW8_002757"
     CDS             170..1324
                     /locus_tag="AC1LW8_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIP4774499.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          517 a          225 c          224 g          499 t
ORIGIN      
        1 gtaaccactt tattcatttt gaaaagtagt tactacagtg tatttagaca tttttcgttt
       61 ttcaaataaa gcttaagtaa tttacccttt tttgctttaa aagtatgtta tgatattatt
      121 aatttctaaa tttttagaca atatttgatt tatatttgag gtgtcacaaa tgaaactttc
      181 tttaaattct aattctaaat atttaagagc accaagtatt cgccaatttt caaatcgtat
      241 gaataatttg gatgattgtg ttaatttaac tattggccaa ccagacttcc cgatgcctga
      301 tgttgtgaaa aaggcatata ttgatgctat taacaatgac aagacatcat actctcacaa
      361 taaagggtta ttagaaactc gcgaagcaat tagtcaatac ttcaaaaatc gttatcattt
      421 ttcctatgat cctgaagaaa ttattgtgac aaatggagca agtgaagcaa tagacacaac
      481 gttacgttct atcatagagc ctggagatga aattataata ccgggaccaa tttatgcagg
      541 ctacatacca ctcatcgaag tactaggtgg taaaccaatt tatattgata caacagcaac
      601 acaatttaaa attacacctg atgcattaga aagtcatatt tctccaaaga caaaagctgt
      661 cttgttaaat tatccgacta atccaactgg cgtagtttta aaaagaaatg aagttttaaa
      721 tatcgtaaat gtattaaaaa aatatccgat atttattatt agcgatgaga tttatgctga
      781 aaatacattt agtggtaaac atgtatcctt cgctgaattt gaagacattc gtgatcaatt
      841 aatattaatt ggtggtttaa gtaaatcaca ctcagcaacc ggaataagaa ttggttttct
      901 attaggacct caatatttga ttgataaatt aacatttatg cacgcctata attgtatttg
      961 tgccaatgtt ccagctcaaa tagcatgtat aacagcactt aacgaaggat tagaagctcc
     1021 taaatatatg aacgaagctt atgtagaacg aagaaattat ttagtatctg aattaactaa
     1081 attaggtttt gagattaccg ctcaacctga aggcgcgttt tatattttcc caagtattaa
     1141 acatatcacc gatgacgatt tcgaattttg tgtcgattta cttgaatcaa cacatttagc
     1201 aatcgttcca ggctcatcct ttacagaatt cggtaaagga tttgtaagga tttcctatgc
     1261 ttatgaaatg gatgttttaa aagaaggtat gaagagactt gctaaatatt taaatactaa
     1321 ataaaaatga tatgttgaac tggacgtcgt gaactaaatt catagtacgt tcagtttttt
     1381 atattctata agttttaaat aatactctat agagactgta aatactaccg agttatattc
     1441 ctctccttta aacacctcac tcaaa
//
LOCUS       DBNENW010000284         1417 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.288, whole genome shotgun sequence.
ACCESSION   DBNENW010000284 DBNENW010000000
VERSION     DBNENW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.288"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            47..>1417
                     /locus_tag="AC1LW8_002758"
     CDS             47..>1417
                     /locus_tag="AC1LW8_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP4774500.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQR"
BASE COUNT          400 a          237 c          258 g          522 t
ORIGIN      
        1 gcttacacac aactttttta tttgctttat cctgaggagg aaaattatgg caagaaaatt
       61 gcatagagag ttgaataaca gacacatcca attgatagca attgggggcg caattggaac
      121 tgggttattc ctaggatcag gtcaaacaat atctttaact ggtccatcac tgttattcac
      181 atacatgatt attggggttg tactattcgc ttttatgcgc gcattaggcg aattgttgtt
      241 gagcaataca agatttaatt catttgttga tattgcaaat gaatatttag gcccttttgg
      301 tggctttgtc attggctgga cttactggtt atgttggatt gtatcaagta tgtcagacct
      361 aactgcgatg ggacaatact ttgcattttg gtatccacaa gtcccaaatt ggattaccgt
      421 gctatttatt gttttaatct tgattagctt caacttatta ggtgccagat tatttggtga
      481 actggagttt tggttctcta ttattaaagt tgtcacaatt attgcgatgg ttatcgttgg
      541 tcttgtatta atctttttct catttaaaac acattatgga catgcatcat tcacaaactt
      601 aatcagtcac ggtggcatgt tccctggagg aacatttggt ttcttaatgt cattccaaat
      661 tgctgtatat tcattcattg gtattgaact tataggtgta actgctggtg aaacgaaaga
      721 tcctgaaaaa accttaccga aagcaattaa taatgtacct atccgtattt tattattcta
      781 tatcggtggt ctattagtaa ttatgtcagt cataccttgg aatgatatcg atccaaatag
      841 tagccctttc gttaaactct ttacattaat cggtgtgcca tttgcagcag gtgtcgttaa
      901 ctttgtcgtg ctaactgccg cggcctctgc tacaaatagt ggtatctatt cgaatagtcg
      961 tatcttattc ggactgtcac aacaagggtt aggtcctaaa gttttaaata aaacgaatag
     1021 tcatggcgtg ccttatttat caatgttagt ttcatcaatt gcattactta tagcagcctt
     1081 gttaaactac attttcccta atgcaattca actattcata tacgttacaa cgttatcaac
     1141 tgtgttgttt ttagttgttt gggcaatgat tattgtcgct tatctaatgt atttgaaaaa
     1201 gcatcctgag gcacataaaa acagtaaatt taagttgatt ggtggcaagc ctattgctta
     1261 cattattcta gcgttcttct tctttgtatt cattttatta ttctttagtg acgaaacaag
     1321 agcagctatt tatatttcgc cgttctggtt tatattttta tttttctttt ataaaaaata
     1381 caaaacgaat gctgaaaaac tcgcatatga acaacga
//
LOCUS       DBNENW010000285         1413 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.289, whole genome shotgun sequence.
ACCESSION   DBNENW010000285 DBNENW010000000
VERSION     DBNENW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.289"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            363..761
                     /locus_tag="AC1LW8_002759"
     CDS             363..761
                     /locus_tag="AC1LW8_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FPRL1 inhibitory protein FLIPr"
                     /protein_id="HIP4774501.1"
                     /translation="MKKNITKVIIASTVIATGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERADKLIKEADEKNEHYKGKTVEDLYVIAKKMGKGNTIAVVKIKDGGKNGY
                     YTFDITRPLEEHRKNIPVVKNGEIDSITWY"
     gene            742..813
                     /locus_tag="AC1LW8_002760"
     CDS             742..813
                     /locus_tag="AC1LW8_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774502.1"
                     /translation="MRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1131..1412)
                     /locus_tag="AC1LW8_002761"
     CDS             complement(1131..1412)
                     /locus_tag="AC1LW8_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HIP4774503.1"
                     /translation="MAAISLTVSTFAGESHAQTKVEKYNEYQTNFKKQVNKKVVDAQK
                     AVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYNTLK"
BASE COUNT          510 a          214 c          198 g          491 t
ORIGIN      
        1 tctttgtttt ataaacatta attatcaaaa aagctatatg tataacaatt tggagtgttt
       61 gtctacccaa cattgcaaac aaaatgcaaa gaaatatata tcgttttaac aataaaaatt
      121 atccaatcat agttgagtga cgtttactac tattattata ctatcaacaa attcaataat
      181 acctcaaata aaaataatat catcaaattc atcattattc tttttcgatt gcattaaacc
      241 ttattaattt gcacttaatt ttaattaatg aagtgttaac tggtaaatgc atgaaaccgt
      301 ttgtaaaata gcagtgtact cgcattagta agtacaaatt tattttacag ggagaactta
      361 ttatgaaaaa aaatatcaca aaagtaatta ttgcatctac agtcatcgct acaggtcttc
      421 taactcaaac taatgatgct aaggcgttct ttagttatga atggaaaggt ttagaaatcg
      481 ctaaaaattt agcagaccaa gctaaaaagg acgatgaacg tgctgataaa ttaataaaag
      541 aagccgatga aaaaaacgaa cactataaag gtaaaactgt tgaagactta tatgtaatag
      601 ctaaaaagat gggtaaaggc aatacaatag ctgtagtaaa aatcaaagac ggtggtaaaa
      661 atggttatta taccttcgat atcactcgcc cattagaaga acatagaaaa aatattccag
      721 tggttaaaaa cggcgaaatc gattcgatta cttggtatta aaaaacatac tgaattaaat
      781 agttgtacgc caaacgttag aaaacaatgc taacgttttg gcgtgctttt tttattttaa
      841 gtaacttcta atttatttag catctttacg actgtttaat aaagcacgta tgattaacac
      901 ggttgccact atatccgtta caatttttat gattgttaat acattcgatt tccttttcac
      961 aatagccacc tcgcttgttc aaaacataaa caactattgc attcactttt aagtaattat
     1021 ttatattatt tatcccaata agctcaccat tcaaataaac acaatattat aaaataatta
     1081 tacctttgga tatagcaaaa agccacactc tatagcatgg cttctatcaa ttattttaaa
     1141 gtattatatt ttaaaactag atcgatttgt ctttgtaatt ttttcttttc atagctgtgt
     1201 tggaaatgaa ttaagttaac agctctttgt gctttacggt gtgttgcaac agttcttgta
     1261 cgtttgaaca agtttacagc tttttgtgcg tccacaactt ttttatttac ttgtttttta
     1321 aagtttgttt gatactcgtt atatttttca accttagttt gtgcatgaga ttcaccggca
     1381 aacgttgata ccgttaaact aatagcagct atg
//
LOCUS       DBNENW010000286         1392 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.290, whole genome shotgun sequence.
ACCESSION   DBNENW010000286 DBNENW010000000
VERSION     DBNENW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.290"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            86..>1392
                     /locus_tag="AC1LW8_002762"
     CDS             86..>1392
                     /locus_tag="AC1LW8_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HIP4774504.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQSNPEDLKVSEVTRFNNKSIGKNELTGALQLKNKVS
                     FKNDFEFNIRVANNHQSVTTGADGWGFLFSKGDGNEYLQKGGILGPKGMENSAGFKID
                     TGYNFKDPMDKEEKQAGQGFKGYGTFVKTGADGTTAKVGTNIPTRGKADNSFQYADNS
                     IDTTDGKFHGQLLNNLKLAYNEKSGIMRAEYAGKIWEANISDLGLDKSEAYNFLITSS
                     QRQGTSQGVYANGWMRTDLNNSTFKLTPNLVNKTETKTVEIPF"
BASE COUNT          570 a          189 c          277 g          356 t
ORIGIN      
        1 cattgtgcaa ctcctattgt atttgtaaaa ttacatgtaa attcactgta agtaaagtgg
       61 aaaatatgga aaaaggagta tgcaaatgag agataagaaa ggaccggtaa ataaaagagt
      121 agattttcta tcaaataaat tgaataaata ttcaataaga aaatttacag ttggaacagc
      181 atctatttta attggctcac taatgtattt gggaactcaa caagaggcag aagcagctga
      241 aaacaatatt gagaatccaa ctacattaaa agataatgtc caatcaaaag aagtgaagat
      301 tgaagaagta acaaacaaag acactgcacc acagggtgta gaagctaaat ctgaagtaac
      361 ttcaaacaaa gacacaatcg aacatgaacc atcagtaaaa gctgaagata tatcaaaaaa
      421 ggaggataca ccaaaagaag tagctgatgt tgctgaagtt cagccgaaat cgtcagtcac
      481 tcataacgca gagacaccta aggttagaaa agctcgttct gttgatgaag gctcttttga
      541 tattacaaga gattctaaaa atgtagttga atctacccca attacaattc aaggtaaaga
      601 acattttgaa ggttacggaa gtgttgatat acaaagcaat ccagaggatc taaaggtatc
      661 tgaagtcact cgtttcaata ataaaagtat tggaaaaaac gaattgacag gagcattaca
      721 attaaagaat aaagtttctt ttaaaaatga ctttgagttt aacattagag tagctaataa
      781 tcatcaatcg gtaacaactg gcgctgatgg atgggggttc ttatttagta aaggtgacgg
      841 taacgaatat ttacaaaagg gaggtatatt aggtcccaaa ggaatggaaa attcagcagg
      901 atttaaaatt gatacagggt ataattttaa agatcccatg gataaagaag agaaacaggc
      961 aggacaagga tttaaagggt atggtacgtt tgtaaaaaca ggtgctgatg gaactacagc
     1021 aaaggttgga acaaacatac caacaagagg taaagcggat aattcattcc aatatgcaga
     1081 taattctatt gatacaacag atggaaaatt ccatggtcaa ttacttaata atttaaaatt
     1141 agcgtacaat gaaaaatcgg ggataatgag agccgaatat gctggtaaaa tttgggaagc
     1201 taatatatca gatttgggat tggataaatc tgaagcatat aactttttaa ttacttctag
     1261 tcaaagacag ggaacatctc aaggtgtata tgcaaatggt tggatgagaa cagatttaaa
     1321 taactcaaca tttaaactaa cgcctaatct agttaataaa acagaaacaa aaacagtaga
     1381 gataccgttc ga
//
LOCUS       DBNENW010000287         1384 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.291, whole genome shotgun sequence.
ACCESSION   DBNENW010000287 DBNENW010000000
VERSION     DBNENW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.291"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..193)
                     /locus_tag="AC1LW8_002763"
     CDS             complement(<1..193)
                     /locus_tag="AC1LW8_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIP4774505.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVT"
     gene            complement(329..562)
                     /gene="sosA"
                     /locus_tag="AC1LW8_002764"
     CDS             complement(329..562)
                     /gene="sosA"
                     /locus_tag="AC1LW8_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-induced cell division inhibitor SosA"
                     /protein_id="HIP4774506.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            705..1328
                     /gene="lexA"
                     /locus_tag="AC1LW8_002765"
     CDS             705..1328
                     /gene="lexA"
                     /locus_tag="AC1LW8_002765"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIP4774507.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
BASE COUNT          468 a          217 c          219 g          480 t
ORIGIN      
        1 gtgttacatc attaccttct ggatcaatta ctttagtatt ttcaatttgt tgtttaaacc
       61 cttttctaaa actctcaaga taagcttttc ttaaggctgt ttgctccttt gcttcttctt
      121 gagttaatcc tacttctttt ttctttttag ctaactcgtt aattctttcg atattcaaat
      181 cagaattact caattaaaaa acctaccttc aatttgttta ttcaatcatt ataatacttt
      241 aacaaaatat aacaaattta agatcagcaa aaaagtaata aaaaacgcta tttcgcaaaa
      301 ataggcaaaa taacgttgtg acaatgtatc aatttattaa agcgaacact ttcccatctc
      361 tttgttcgct attgtttgta gggtctttat gttcgtatgc tttatttatc gtattgttct
      421 taatttgatg gtcagtcatt tcgtacactt gttccgagtg agcactaatg ttcgcactta
      481 acaagaatat tgcaaaggct gcacttgaaa ctaaaaatat gatgatatat gttgatacgt
      541 ttttatattt attgtaaaac atcattttca ctcctagaac atttgtttgt attagtaatt
      601 tatcagaact tatgttctgt gtcaatattt taacgaacaa atgtttgttt tggtttcaaa
      661 ttaatgatat actgtattta aattaattta gggagtgcct atatatgaga gaattaacaa
      721 aacgacaaag cgaaatatat aactatatta aacaagttgt tcaaacgaaa ggttatccgc
      781 ctagtgttcg cgaaattggt gaagcagttg gcttagcatc cagttcaact gttcatggtc
      841 acctttcacg tcttgaagaa aaaggctata taagaagaga tccaacgaaa ccacgtgcta
      901 tagaaattgt aagtgatcaa acaaatgata atattaatat ggaagaaacg attcatgtgc
      961 cagttattgg taaagtcaca gcaggtgttc ctattaccgc agtagaaaat attgaagaat
     1021 attttccatt acctgaacac ttaacatcga cacacaatag cgacatattc atattaaacg
     1081 tcgtaggcga cagtatgatt gaggctggta tattagacgg agacaaagta attgttcgca
     1141 gtcaaaccat agcagaaaat ggagacatta ttgttgctat gactgaggaa gatgaagcaa
     1201 ctgtcaaacg cttctataaa gaaaaaaatc gttatcgatt acaacctgaa aatagtacaa
     1261 tggagccaat ttacctagac aatgttgctg taattgggaa agtaattggt ttgtaccgcg
     1321 aaatgtaata ttttaaaccg ttatatatta tcgtaattgt taagccctca tttttataaa
     1381 tttt
//
LOCUS       DBNENW010000288         1370 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.292, whole genome shotgun sequence.
ACCESSION   DBNENW010000288 DBNENW010000000
VERSION     DBNENW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.292"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(391..1002)
                     /locus_tag="AC1LW8_002766"
     CDS             complement(391..1002)
                     /locus_tag="AC1LW8_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIP4774508.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
BASE COUNT          524 a          204 c          219 g          423 t
ORIGIN      
        1 gaatacgagt atttttgtag catgcaaatg gcattgctac atttattaaa atgcgcaatt
       61 tgttatgagt gacttttttg aatagaaggc atattaaagt gacgaaaaca gtttgtgtgg
      121 aagacagggt aaattctgct tatttaagat gatagtttgt attatagatg gattgtgaga
      181 tgcgtttgcg gaataaatgg tacatactga gggaactgaa cttttatggg aaacttcatt
      241 atatctctta acgtaaaggg ttttagatga ctttcaacta aagtatgcca aataaaccta
      301 aaaaagcagt aaatcattcc agccatcgaa atgattttac tgctttttat ttatagcaat
      361 tgaaaatata ccattatact aacaacaata ttattttact ctcttacgtt tgccaatgta
      421 acgtatagca aagtaaatga ctgcgataat gatgacaaca tacataatac gtgaatatgt
      481 atgaagacca gtcatcaaca taccaaagct atcgctcaac gtgcgtccta gtaaaattaa
      541 accgaaattc caaattgttg tacctattaa tgaaataacg gtaaatgtca caacattcat
      601 gcggtttaca ccggctggaa tggtaatcaa tacacgtaat acaggtatga aacgacagat
      661 aaatacagcc catacgccat actttttaaa ccaatcattt gctcgcttta aatccttact
      721 tttcaattta atccacttac cgtgtcgatc aataaagcga tatagacgtt cttctgaaat
      781 taaacgacag atataatata aaattaacag ccctataaac gatgcgatgg ttgcaataat
      841 aaataaggtt aaaattgata aatgtgattt aacagacata aggccagcaa atgtcagaat
      901 aatttccgat ggtacgatag gtaaaacatt ttctaataaa attaatataa aaatggctgc
      961 atacccaaaa cgactaataa attcagtgat aatttgttcc attattaaaa ataagctcct
     1021 ttaattttca gcaattgcag acaaatagaa actggataaa ttcaaatgaa taatgaatga
     1081 tgtcgtatgc gatgttgaaa caagcatttg tcttaaaatg tattataagc aataaatcaa
     1141 aaaattgctt gctataagta cgaaaagtca acaaatgatc atctaaataa caggcgctgc
     1201 acaatgaatg agcaatgtag ttttatgcaa tttaaaatga atgtctaata tgtaagaaag
     1261 cctgcaatat acgttttaaa acactaggat tgcagtattt ttaattaaca aatgataggc
     1321 tactatgtaa ctaaaagttc ctttatatcg ttttaatagc aaaagttaag
//
LOCUS       DBNENW010000289         1358 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.293, whole genome shotgun sequence.
ACCESSION   DBNENW010000289 DBNENW010000000
VERSION     DBNENW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.293"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            230..334
                     /locus_tag="AC1LW8_002767"
     CDS             230..334
                     /locus_tag="AC1LW8_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774509.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKSKI"
     gene            463..714
                     /locus_tag="AC1LW8_002768"
     CDS             463..714
                     /locus_tag="AC1LW8_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774510.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            787..1358
                     /locus_tag="AC1LW8_002769"
                     /pseudo
     CDS             787..1358
                     /locus_tag="AC1LW8_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503679.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          548 a          164 c          233 g          413 t
ORIGIN      
        1 ttccattata ttgttatagc tttttatatt tgtataatga acatataagt ttaagaagat
       61 gagagtgaag gaaataaaaa agctcaaatg taccaaattg ttaatcttaa taaatctcta
      121 ctttataaag attgaatgga cattcgagcg ttaatcagtc aggagggaat ttcactccta
      181 caatttaata ataatacttg cttcaccact gtacaaggag tgatttgtta tgttcaaagt
      241 gaattattcg attttaagtt attatccaga atataatatc gcagtaagtt ggcaacgttt
      301 aagagaagga aaaacaataa aaagcaagat ttaatactgc tgcgtcatga ggcgcttgaa
      361 cattatttga tgaataagta taatttcaac tatgattatg cacataaaat tgtatcaaaa
      421 aaatacgatt attcaatttt tataaaaaag aaggtggatt aaatgcttac attaataaaa
      481 ttgaaagaag atgaacaggt tataatatat gaatatatac ctgaagatga tataagtaac
      541 ggtaaaggtt cagtaacttt taataaaaaa gatgcagagg ttatagattt ctcattatct
      601 gaaatagaaa atgaagaata ttttatgtta tatcgtaata agtctttttc tgtagtaaga
      661 gactttatcg agaagcaaga atttcccgaa aattataaaa tagcatggta ttaatgcatc
      721 ctttctagac agataaagag aaagtggtgc tatttttata tgctttttta actatttact
      781 gaaatagcag aatcacaaaa gttaacatat ttaaaagaac ttggtgaaga tggcgaatat
      841 aaatatgttg ccaaaataga tagtaaaacg tctaaactat gtcattcgct caacggcaaa
      901 gtatttaaag ttaaagatat gataccaggt gtgaatgcgc cacctatgca tccttggtgt
      961 agaagtacca cagtgccaca tgttggcaat tggtgagaca agttctttaa agagcgtgaa
     1021 ggtaaatatc aagtagaaga tgacacaacc aaagatgaat tacaacaagc taaagtatta
     1081 ggaaagaaaa tatatattac tgatcaagca attgataaag tcagatatgt tgatattcca
     1141 acacatacca aagaagaaaa tcaatttata caagaacaac ataaagcctt gcttaaagat
     1201 gccaaagaaa acaatgatag caatgaagta gcttatttat taaaagatgg taaagttaca
     1261 aaagtatatg gtgatcaaga tagtgtatct tttgcaccag gggaaaaagc aacagaattg
     1321 ttatttaaca gtaaaccgaa ttcaattgtt atgttaca
//
LOCUS       DBNENW010000290         1316 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.294, whole genome shotgun sequence.
ACCESSION   DBNENW010000290 DBNENW010000000
VERSION     DBNENW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.294"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(75..1151)
                     /locus_tag="AC1LW8_002770"
     CDS             complement(75..1151)
                     /locus_tag="AC1LW8_002770"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIP4774511.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT          415 a          264 c          168 g          469 t
ORIGIN      
        1 cttcgcttca ttgaaaggga ttttagtcat gattaacttt tacatattgt tttcatgatt
       61 atattcaatt tttattaata ttttggtaca acgactctcc aaccattttt atcttctaaa
      121 gtaccatttt gaataccagt atagacgtcg tataattttt gagtaatttc accagtctca
      181 ttattattaa taacgatttc acgatcttcg tatctcaatg tacccacagg tgaaataact
      241 gctgcagtac cactaccaaa tacttctgtt aactcacctt tatcatatga ttcgaataat
      301 tcatcgattg aaacgcggcg ctcttcgact tcatatccta agtttttagc taattcgata
      361 atagatttac gtgtaatacc aggtaaaata ctgccattca actctggtgt aattactttt
      421 ccattttcaa cgaagaaaat attcatgcta ccaacttctt cgatatattt ctgttcaaca
      481 ccatcaagcc ataatacttg gtcataacct aatttatttg catttgtttg tgctaataaa
      541 cttgccgcat agttacctgc aacttttgca aagcctacac cgccacgaac agcacgcaca
      601 tattcatctt ctacatagat tttagttggt tttaaagtct caccaccata atatgcacct
      661 gaaggagata aaataattaa taatttatac tgatgtgatg caccaacgcc aagtgcccct
      721 tctgttgcaa aaacaaatgg acgaatatat aatgattgac cttccccttc aggaatccaa
      781 tctctttcta tatcaactaa ttgttttagc ccctctaaca attctgcttc gtctacttga
      841 ggcatttcta atcgtgctaa cgagttatta agacgcttaa aattttcttc aggacggaaa
      901 agtgcaactt ccccatctct tttatatgct tttaatcctt cgaataccga ttgaccataa
      961 tgaacacctt gtgcagcagg tgaaatttca ataggaccat aaggtactat cttcaaatca
     1021 tgccatcctt tatctgcatc ataatcataa ctcaacatat aatcagtaaa atatttacca
     1081 aaacctagtt gagatgtatt tggtttttgt tttaatgttt ctcgtcgttc aactttaact
     1141 gcttgtgaca tggtgattgc ctcctaataa tattgtataa gaatttgttt aacttaaatt
     1201 ataacaatcc atattttgct gttcaacaaa ttttctaaaa attcaaaatt aattaacaga
     1261 tttctagaaa gactatatct tttagtataa acgtattaat ttcacagaga caagta
//
LOCUS       DBNENW010000291         1310 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.295, whole genome shotgun sequence.
ACCESSION   DBNENW010000291 DBNENW010000000
VERSION     DBNENW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.295"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..1310
                     /locus_tag="AC1LW8_002771"
     rRNA            <1..1310
                     /locus_tag="AC1LW8_002771"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          340 a          295 c          395 g          280 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac
//
LOCUS       DBNENW010000292         1303 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.296, whole genome shotgun sequence.
ACCESSION   DBNENW010000292 DBNENW010000000
VERSION     DBNENW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.296"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(130..1155)
                     /locus_tag="AC1LW8_002772"
     CDS             complement(130..1155)
                     /locus_tag="AC1LW8_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIP4774512.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          426 a          244 c          172 g          461 t
ORIGIN      
        1 ggaatgatgt aattttataa catcaacggc ttcaaatgag tagtttcata tataaatcaa
       61 taaacacaac tactcaactg aactggctta attaataaat gatttgccag acgaaatata
      121 acagcattat tagattaatt ctaatagttt atttaaattt tcttcggttg tcgcccagct
      181 ggttgcgaat ctaacaacac gatgttgatc atcgtatttt tcccaaacag caaatttaac
      241 tttttgttct aactctgcta ttttctcgtt acttaaaata aaaaattgtt gattggttgg
      301 agaatcaaag taaagacgat agcctttatt tataaacccg tctttcatct tatttgccat
      361 ttcgatagca tgtctgctta tattaaaata taaattatcc gtaaataatt ctaaaaattg
      421 tatgcctgtt aaccgtcctt ttgctaaaag tgcaccgtga tgtttgattc gagtggtaaa
      481 ttgtttcggt tcattattct tcgtaaaaac aattgcttct ccgcataatg cacctatctt
      541 cgtaccacct atataaaata catcacaata tttagcgatg tctttaatag tcatatctga
      601 ttgatcactc atcaatccat accctaatcg tgcaccatcc ataaataatg gaagctgata
      661 ttgcttacat actttgcata actcttccaa ttctgattta gagtataatg tgccatattc
      721 tgtaggatga gaaatatata ccattcctgg gaataccata tggtcctttt taaaatcact
      781 tttaaatgtc tccatgtaag tttcaacatc tgaagcacta acttttcctt ccttagaggg
      841 tatagtaatt actttatgtc cactatattc aattgcaccg ccctcatgca cagcaacatg
      901 accagtgtct gctgaaataa ccccttcgta actttctaac attgaattaa taacaacctg
      961 attggtttgc gttccaccta ctaaaaaacg gatagtagca tttggacaat caattgtatc
     1021 tttaatcttt tcaattgcct gagctgtgaa ttgatcaaag ccatatcccg aagcttgtac
     1081 aagatttgta tctactaatc gttttaatac tttttcatga gcaccttcta aataatcatt
     1141 ttcaaatgag atcactacat ttccccctaa aactaatatc agcattttaa taagataaac
     1201 taatttcaaa actagttcga tatttaaaat gtattgtgaa ttgttaaagt ttgtatcgca
     1261 ttatcgcgaa gttgaataaa tatattatac atcaaacaat aca
//
LOCUS       DBNENW010000293         1285 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.297, whole genome shotgun sequence.
ACCESSION   DBNENW010000293 DBNENW010000000
VERSION     DBNENW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.297"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            192..1190
                     /gene="rbsR"
                     /locus_tag="AC1LW8_002773"
     CDS             192..1190
                     /gene="rbsR"
                     /locus_tag="AC1LW8_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose utilization transcriptional repressor
                     RbsR"
                     /protein_id="HIP4774513.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
BASE COUNT          425 a          247 c          207 g          406 t
ORIGIN      
        1 taaaagttat ttaaccttaa taatatattc tctcgcctca accttaatcg catactatag
       61 gcgtctgttt gtagacaata aaagtcattc acgtcttcat atgtcatcaa atgtttatca
      121 tgatatgatg aatataataa tcgggtatat aactgtatga ttaattacac aataaagaga
      181 aggaatataa tatgaaaaaa gtgtcaatta aagatgttgc tagagaagct ggtgtatcag
      241 ttacaactgt gtcacatatt ttaaatcata atgatagtcg tttttccgca acaacgataa
      301 aaaacgtaca tgctgtttca gaacgtttag gctatgcccc taataaacat gcaaaacaat
      361 tgcgcggcag taaaattcaa actattggcg tcattttgcc tagcttaaca aatccgtttt
      421 tctcagcact gatgcaaagt attcatgacc ataaaccatc tgatgttgat ttatgctttt
      481 taacatctac agcaactgat ttgtatgaca atattaaaca tttaattgat cgaggtattg
      541 acggattaat tatcgcacaa tacatttcat ccccggacgc cctaaataat tatctaaaga
      601 aacatcatgt accttatgtc gtactggatc aaaatgacca tcaaggctat acagattttg
      661 ttcggacaaa tgaatatcaa ggcggacaac ttgcagcaca acatttaata gaactcggtc
      721 acaacaatat gatgattgtt gcaccatatg acatgatggc gaatatgtcg actcgtgtcg
      781 ctggatttgt tgatactttg cgcgcgaatc aattgccaga accacaaatc gtccatactg
      841 aattatctaa gcgcggtggg ctaaccattg ttgatgacat catggttcaa tctgccactg
      901 caatcttcgc tattaacgat gaactcgcta ttggcatttt acgaggacta attgaacatg
      961 gcatcagtat tccgaaagat atctcattaa taggttatga cgacattgat tatgcagcgt
     1021 acgtctcgcc acctttaact actgtggcac aacctataac tgatattggc aaaacatctt
     1081 taaccttatt acttcaacga ttacagcact tagataaatc cattgatatg attgaattac
     1141 caacgacttt aaaaattcgt gcaacaactg gctatcatct ttcaaactaa ctacgtatct
     1201 tccgaaatat actcatcatt gttatgccct tagcgttgct ttaatgctga gggtttttaa
     1261 tcataattat ttcactatga aatta
//
LOCUS       DBNENW010000294         1237 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.298, whole genome shotgun sequence.
ACCESSION   DBNENW010000294 DBNENW010000000
VERSION     DBNENW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.298"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            141..>1237
                     /locus_tag="AC1LW8_002774"
     CDS             141..>1237
                     /locus_tag="AC1LW8_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIP4774514.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENI"
BASE COUNT          490 a          131 c          204 g          412 t
ORIGIN      
        1 tgaaacttct gtttcagttg tttggataaa tagatattta tatagttttt aaagaaaaaa
       61 agtgataata tttagctaat ctaatgaaaa tggatttatt ggacaaaaaa tacattttaa
      121 atgagtagag gaggctgcaa atgggttaca gaactttaaa aagtattttt catgaacaca
      181 atgaaagtaa aatgaaggaa gagtatacta aaagatttaa ttctttagct tctttcaata
      241 ctaatattaa tatcatacct atggaaaatg gaaaaaaagt taatgatttg gaatatcctc
      301 tattctttat ggtgactaaa aatctatcaa aaaaacaaga attaatatca attaatagta
      361 gaaaaattga tagagcactt aattctttac catatgcagc tagagaacaa tattttaatg
      421 atttattaat cgatgaatta caaagtacta atgaaattga aaatgtattt agtactaaac
      481 aagagattgc acatgcgtta aataaccaag catcagaatt tcttaagttc agaggcctcg
      541 tggatcaata taaagagata gaacttaata aaaaaattaa agttgataat gtaagagaca
      601 ttagagcgat ttatgataaa ttagtttcaa atgaaattaa cgaacaagat aagttagatg
      661 gagagctatt tcgtaaaaat tttgtcggtg tgcatgatgg gtcaacgaat aaatatatac
      721 atgttgggtt acaacctgaa accaaaattg ttgaatatat aggtgaaatg ctaacatttt
      781 taaaatattt tgatgcgcct cagccgttca aaatcatggc tagtcattat ctgtttgaat
      841 atatacatcc tttttatgat ggcaatggta gagttggaag atttataatt gccaagctct
      901 tgagtgatta ttatgataat tatactgctt tgacattttc atatgttata aatagaaata
      961 aatcgaaata ttacaaggcg tttatgactg cttcaaatca tttgaattgt ggtgatttga
     1021 cggaatttat tgacacaatg cttgagctat tgatagcagg acaagaaaga atacttgatg
     1081 agttaatacc aaaaatggat gcgactgaaa aattaacgct ttatttaact agtcattata
     1141 aacagataga ttatgaattt ttgtatctcc tttcaatgga taaattgttt ggaaataaaa
     1201 gaaatagatt aacattaatt gatttagaaa acatatt
//
LOCUS       DBNENW010000295         1211 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.299, whole genome shotgun sequence.
ACCESSION   DBNENW010000295 DBNENW010000000
VERSION     DBNENW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.299"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(502..1146)
                     /locus_tag="AC1LW8_002775"
     CDS             complement(502..1146)
                     /locus_tag="AC1LW8_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIP4774515.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
BASE COUNT          458 a          184 c          136 g          433 t
ORIGIN      
        1 atccaattga aaacacacta ctttttagta ttttcaaaat aattttaaat gaccacatct
       61 acaacgtatt actattatct tttgttagtt atatattcat tttcaattta aaaataaaca
      121 cttatttaaa tcacattcta cttttgagat gagctcaaac taacattaaa ttgtttatat
      181 tatctatact tcttcctatt aatgtttcga ttttaaaatt aatacgtagc tatactttac
      241 ttttctaata aaagtgtaat tagaaaggca agtacacctg tttaaagttt atcttctaag
      301 atttgtttac tttagaacta tagcagttaa tcatttcaat gattaataat atctttacaa
      361 ttaaggtaat atatttttat tgctttatca gtaaaatctc agtctaagtt tctaacttat
      421 gacaatcgct ccagtttaag gttgatattc attattgatt attccaaaac tttatacaaa
      481 tttctttatt aatttaactg tttatcttct gaacatatta gcaagataaa gtatatcctt
      541 aataatattc aacaaattaa taaatagatt aaatcccatt tctcttggtg aatagtcacc
      601 tctttttaaa cgattaaaat catacaaagt ataaagtaga aataacaaca aactaacgac
      661 ggtaataata gtgtaaaaaa taggattttg aagaaaaata ccaattagac tcgcaataat
      721 taatgttatt aatgtaacga ataaatattt gcctatactc gatgcatctc caacgaagaa
      781 ataaccaata ataccaaatg ctataaatgc aaaaattgct aatgcgatat ttttatagaa
      841 aatatctggt cctaaatttt gtaaatacgt ggtaaacgtt gcgtatgaca ataagccgat
      901 cactacagcg taaatatgtg aaataattaa accgaaccgt ctcgctcttt caaaaacaag
      961 tgttgctaaa ataataatca gtaatccaaa tgacaagggt tgtcgccaac ttaatggtaa
     1021 aaactgacct agatagcatc ctatgccaaa tataatccaa taatacataa aataaagcca
     1081 cactttactt attgattgag catgctggtt ttgatgataa tatgaatgat ttgtattttg
     1141 cgacaaaata attcctcctt ctaccgtcgt tttatgcgtc aaattttaca gtaaattttg
     1201 cttcatcaaa a
//
LOCUS       DBNENW010000296         1191 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.300, whole genome shotgun sequence.
ACCESSION   DBNENW010000296 DBNENW010000000
VERSION     DBNENW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.300"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            3..1121
                     /locus_tag="AC1LW8_002776"
     CDS             3..1121
                     /locus_tag="AC1LW8_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP4774516.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
BASE COUNT          505 a          146 c          201 g          339 t
ORIGIN      
        1 tcatgaataa acaacaaagt aaagtacgct attcaattag aaaagttagt attggaattt
       61 tgtcaatttc aataggtatg tttttggcat tgggtatgtc gaacaaagca tatgcagatg
      121 aaattgataa atctaaagat tttacaagag ggtatgagca aaatgtattc gcgaaatcag
      181 agttaaatgc taataaaaat acgacaaaag acaaaataaa aaatgaaggt gctgttaaaa
      241 catcggacac aagtttaaag ttagacaaca aatcagcaat ttcaaacgga aatgaaatta
      301 atcaagatat aaagatttca aatactccga aaaactcaag ccaaggtaac aatctagtta
      361 ttaataacaa tgaacctact aaagaaatta aaattgcaaa cttggaagct caaaattcta
      421 atcagaagaa aacgaataaa gttactaata attactttgg ttactacagt tttagagaag
      481 ctccaaaaac acaaatctat actgtaaaaa aaggagacac acttagtgct atagcattaa
      541 aatacaaaac tacagtttca aatattcaaa atacaaataa tatagcaaat cctaatttaa
      601 tatttattgg tcaaaaatta aaagtgccaa tgacaccatt agtagaacca aaaccaaaaa
      661 cagtgtcttc aaataataaa agtaatagta atagcagtac attaaattat ttgaaaacat
      721 tagagaatag aggatgggat ttcgacggta gttatggatg gcaatgtttc gatttagtta
      781 atgtatattg gaatcatctt tatggtcatg gattaaaagg atatggagct aaagatatac
      841 catatgcaaa taattttaat agtgaagcta aaatttatca caacacacca actttcaaag
      901 ctgaacctgg ggacttagtg gtttttagtg gaagatttgg tggaggatat ggtcatacag
      961 ctattgtctt aaatggtgat tatgatggaa aattaatgaa gttccaaagt ttagatcaaa
     1021 actggaataa tggtggatgg cgtaaagcag aggttgcaca taaagttgtt cataattatg
     1081 aaaatgatat gatttttatt agaccattta aaaaagcata atttaaatcg aaggcaggac
     1141 attgaaatat gaaattttca actttaagtg aagaagaatt taccaactac a
//
LOCUS       DBNENW010000297         1163 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.301, whole genome shotgun sequence.
ACCESSION   DBNENW010000297 DBNENW010000000
VERSION     DBNENW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.301"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>1163
                     /locus_tag="AC1LW8_002777"
     CDS             <1..>1163
                     /locus_tag="AC1LW8_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HIP4774517.1"
                     /translation="DYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVF
                     GYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKK
                     YQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDKESKILVPSPLPSHQ
                     SNEWVKNTKVFDETKLFLEIDIDNHRYICLSSKFNFEKREKEIPFEDRDSCYFLAMGY
                     FYNKEDSNEIIKGYENNYDRGINIPRAHSIYLYEYYWSEAYKNYKEGYLTESDGKLCP
                     AIYEYFWELDYSVKDKSISFYIPCKEIVDYFSLIQTEEGVWKTKFGETICINSKLLEF
                     DNECLLIKKESLLNFLNTKKLSIGWKIYLEKISLRDRQEWWYNVFYDDGKYNK"
BASE COUNT          459 a          111 c          198 g          395 t
ORIGIN      
        1 acgattacat aaaatcttta aaaaacaaat tagatagcca tttacatttt tctatcgata
       61 aaattaaaaa ctcaatgaca acggaatatg gaagaggaac tggggcatat ggcgattttg
      121 gcagatatgt atttggttat gctgtacgaa actgggtaaa gggttttaaa agtgatcaag
      181 acttaagtaa tatagcatta atgaggattt ttgaaatggg ttatgatgca aaattacatg
      241 gtgaatttga tatgtgggtt aatcgttatg ataactttaa taattcaatt gaaagaatta
      301 gcaaaaaata tcaatggatt gcttactatg aaattttagc taaacttgta gataaatttc
      361 ctgatgttca atattctgga ctatgggatg attatattag agatattgat cctactttac
      421 tattacttga aattgataaa gaatcaaaaa tactagtacc ctcaccactt ccttcgcatc
      481 aatcgaatga atgggtaaaa aataccaaag tttttgatga aacaaaatta tttttagaaa
      541 tagatataga caatcataga tatatatgtt taagctcaaa gtttaatttt gaaaagagag
      601 agaaagaaat accatttgaa gatagagact catgttattt cttagcgatg ggatattttt
      661 ataataaaga agatagtaat gaaattatca aaggttatga aaataattat gatagaggta
      721 ttaatattcc tcgagcacat agtatatatt tatatgaata ttattggagc gaagcttata
      781 aaaattataa agagggatat ttaactgaat cagatggtaa attatgtcca gctatttatg
      841 aatatttttg ggagttagat tattctgtta aggacaaatc aataagtttt tatattccgt
      901 gtaaagaaat agtagattat ttttcattaa tacaaacgga agaaggagta tggaaaacta
      961 aatttggtga aacaatatgt attaattcta aattactcga atttgataat gaatgtttac
     1021 ttattaaaaa agaaagttta ttaaactttc ttaatactaa aaaattgtct attggatgga
     1081 aaatatatct agaaaaaatt tctcttcgag atagacaaga atggtggtac aatgtttttt
     1141 atgacgatgg caaatataat aag
//
LOCUS       DBNENW010000298         1139 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.302, whole genome shotgun sequence.
ACCESSION   DBNENW010000298 DBNENW010000000
VERSION     DBNENW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.302"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(231..1124)
                     /locus_tag="AC1LW8_002778"
     CDS             complement(231..1124)
                     /locus_tag="AC1LW8_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP4774518.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKELITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          315 a          176 c          219 g          429 t
ORIGIN      
        1 ataataatgt ttaataagtt attggaaatc taataaacta caaaaaatat tttgattaca
       61 taatgattat ttaaaaatgt cggttaactt taaggtgaca agttattgca attttgaagg
      121 tagacatcat attttataat cgaataacat acaaaataaa gcgaccaatt ttgcagtgtt
      181 acgaattgta aactgcgaac tggtcgcttt ttattgatgt catgattaat ttaatggatg
      241 taattatatg atgaaacttc tgaagcagag atggttcttg atgaaacgat atattcacca
      301 atccagttca tttctgaaat tagaatactt ccatcaatat taactttttc aacgtaggct
      361 acatgaccaa atggaccatt tactgtttgt aaaattgatc ctcgtgttgg gtgtctatct
      421 actttgaagc cattgcttga agcttggcct gcccagtttt tagcatctcc ccaaaatgta
      481 ctaatcgtgt gtccatcttt ggcacgttta tcaaagacat accatgtaca ttgtccagca
      541 gtatataagt tgttcttact tgtgataaga ggctgatcaa tgattttacc gttacctaat
      601 gctaaaggtt taccgtcagc ggtctttgct ttgtcattaa attcggcgat ttgtaattcg
      661 tcatacaatt cgtctaaatc gatgcctgta ataagctctt tgttatcttt tgaaaaagcg
      721 ttatttaatt catcgtcatt gtcttcgaca ttcggtattg ctggtgtcaa aggattgctt
      781 ggtgacgttt gaggcggtgt gtgtgaatca attgcgtcat taatgtgcgt atactgacca
      841 cttaatgaag aatggtactg attgttgtta agatcacgtt gatttgcgtg ttgatgattg
      901 tcgtttgtac gtgactggtt ttgatgattg ttgtttggcg tgttgttttt gtcatatgta
      961 taagtatacg cgccggtgtc tttattcact ttgaactgtg cgtttgggtg tgctttcttt
     1021 gcttcttcta atgttttgct atcattcgta tatgcttgag ccgagttagg cgacatacta
     1081 aataaagtaa gagttgtcat cgtcagtaaa attgttttct tcataataac catttaatc
//
LOCUS       DBNENW010000299         1139 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.303, whole genome shotgun sequence.
ACCESSION   DBNENW010000299 DBNENW010000000
VERSION     DBNENW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.303"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            52..1017
                     /locus_tag="AC1LW8_002779"
     CDS             52..1017
                     /locus_tag="AC1LW8_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIP4774519.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
BASE COUNT          397 a          161 c          227 g          354 t
ORIGIN      
        1 tccacttgtg atggactata gctatataga agagacaaag gagatatttc tatgaaaaaa
       61 attatgatta ctggtgcatt aggacaaatt ggtacagaat tagttgttaa gtgcagagaa
      121 atttatggga cagataatgt tcttgctaca gatattaggg aacctgaagc agactcacct
      181 gtacaaaatg gaccatttga aatcttagac gtaacagatc gtgaccgtat gtttgagtta
      241 gttagggact ttgaagcgga tagtctaatg catatggcag cattattatc agcaactgct
      301 gagaaaaatc caattctagc ttgggattta aatatgggtg gattaatgaa tgcattagaa
      361 gctgcaagaa cttataattt gcactttttc acaccaagtt caattggtgc atttggagac
      421 tcaactccta aagttaatac gccacaagta acgattcagc aacctacgac aatgtatggt
      481 gtaaataaag tagctggaga attattgtgt caatactatt tcaaacgttt tggtgtagat
      541 acaagaagtg ttagattccc aggtttaatc tcgcatgtta aagagccagg tggcggtact
      601 acagactatg ctgttgaaat atacttcaaa gcagtaagag agggtcatta tacaagcttc
      661 atagataaag gcacgtatat ggatatgatg tatatggatg atgcaattga agcaattatt
      721 aaacttatgg aagcagacga cgctaaatta gaaactagaa atggttataa tttgagcgca
      781 atgagttttg atccagagat ggtaaaagaa gcaattcaag aatactatcc caattttaca
      841 ttagattacg atgttgatcc tattagacaa ggtattgcta atagttggcc ggattctatt
      901 gatacaagct gttcacgtgg cgaatgggga tttgatccta aatatgattt agcgagcatg
      961 actaaattaa tgttagaagc tattgaacaa aaagatactg ttaaaaataa taactaatca
     1021 tttccattca ctttaataca cggaatgata ttttaaatta ctctttattt taataaacta
     1081 gtgcatgaat tctaatatta ttcattatac atattgaatt cgcgagctta gttcatttt
//
LOCUS       DBNENW010000300         1122 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.304, whole genome shotgun sequence.
ACCESSION   DBNENW010000300 DBNENW010000000
VERSION     DBNENW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.304"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..913)
                     /locus_tag="AC1LW8_002780"
     CDS             complement(<1..913)
                     /locus_tag="AC1LW8_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIP4774520.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDI"
BASE COUNT          355 a          212 c          144 g          411 t
ORIGIN      
        1 caatatcttg tgggtgcata tgtttatgtt ttttaatata ttctaaaagt ttttgtttat
       61 ctataaaggt accttcacta tttctagctt cagtcacacc atccgttaaa atgataatta
      121 aatcatcaag gtatattgga atttcttgtt gttgatatcg tgtttgtgaa ctgattccta
      181 acactctacc tctaactgaa atttcttcga attcttcttt ttcagcgcga taaatatatc
      241 caggctcatg accagctgaa ctacaataca ataaatggtt catttcttca tataaaccat
      301 aaaacattgt gacgaacata ttttgattaa tattcttttc aacaacacga tttaaacgtt
      361 ttaaaccatc actcggtaat tgtgagtgtc cataagaatc catgccaaac tttatcatac
      421 tcattgctaa agcagctggt ataccttttc caatgacatc tgcaacagca aagctcattg
      481 tgccatcgtt atggtcaatt aaattaaaat aatctccact tactttttgt gccgccactg
      541 aaataacgcc aatttgaata ctatcaaatt gtggaatatc tgttttaagc attgtttgtt
      601 gtaagctaga agctaagtct atctctttat cgtgaacttg aagtttatct accaatcttt
      661 gataatctcg ataactataa ccaaagcctt taacgatttc ttgtaagaca tctaatgtct
      721 tgaacacatc ttcacgcgtt aagtttaacg tcaatatata gtttttgtga atatcgacaa
      781 tgtcttcagg caagacgtcc ttacgaatca cttcgtcagt gtatttctca cactttttta
      841 tcaattctac tttatcttgg cacgttaaac tttcatcaat taaaccctta taatgttgct
      901 taaattcttc cacgattagt tgcctcctca cttcccttac aaagcaaatg cttaagcaag
      961 gataattatg attcacttac taacatttat ttaattgcac ttatcaattt tgtcgtttgc
     1021 aattttgctg tagatgatac ttttaagcta aaaaaaactt taggatggaa tacctaaagc
     1081 ctaaaaaact taagacatcc ttttataata aaaaatatga at
//
LOCUS       DBNENW010000301         1115 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.305, whole genome shotgun sequence.
ACCESSION   DBNENW010000301 DBNENW010000000
VERSION     DBNENW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.305"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            148..1107
                     /gene="hyl"
                     /locus_tag="AC1LW8_002781"
     CDS             148..1107
                     /gene="hyl"
                     /locus_tag="AC1LW8_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HIP4774521.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDFGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          427 a          167 c          194 g          327 t
ORIGIN      
        1 catcactcag taatttatca gttgctatat taaatatact aatataaacg attataaata
       61 tttgatatgt ctcaactgca atattctaaa ttgacatatt gattattgtt tcctcaaatc
      121 gtttaaaaaa atagaaggat gatgaaaatg aaaacacgta tagtcagctc agtaacaaca
      181 acactattgc taggttccat attaatgaat cctgtcgcta atgccgcaga ttctgatatt
      241 aatattaaaa ccggtactac agattttgga agcaatacta cagtaaaaac aggtgattta
      301 gtcacttatg ataaagaaaa tggcatgcac aaaaaagtat tttatagttt tatcgatgat
      361 aaaaatcaca ataaaaaact gctagttatt agaacgaaag gtaccattgc tggtcaatat
      421 agagtttata gcgaagaagg tgctaacaaa agtggtttag cctggccttc agcctttaag
      481 gtacagttgc aactacctga taatgaagta gctcaaatat ctgattacta tccaagaaat
      541 tcgattgata caaaagagta tatgagtact ttaacttatg gattcaacgg taatgttact
      601 ggtgatgata caggaaaaat tggcggcctt attggtgcaa atgtttcgat tggtcataca
      661 ctgaaatatg ttcaacctga tttcaaaaca attttagaga gcccaactga taaaaaagta
      721 ggctggaaag tgatatttaa caatatggtg aatcaaaatt ggggaccata tgatagagat
      781 tcttggaacc cggtatatgg caatcaactt ttcatgaaaa ctagaaatgg ttctatgaaa
      841 gcagcagata acttccttga tcctaacaaa gcaagttctc tattatcttc agggttttca
      901 ccagacttcg ctacagttat tactatggat agaaaagcat ccaaacaaca aacaaatata
      961 gatgtaatat acgaacgagt tcgtgatgat taccaattgc attggacttc aacaaattgg
     1021 aaaggtacca atactaaaga taaatggaca gatcgttctt cagaaagata taaaatcgat
     1081 tgggaaaaag aagaaatgac aaattaatgt aaatt
//
LOCUS       DBNENW010000302         1078 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.306, whole genome shotgun sequence.
ACCESSION   DBNENW010000302 DBNENW010000000
VERSION     DBNENW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.306"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(3..1013)
                     /locus_tag="AC1LW8_002782"
     CDS             complement(3..1013)
                     /locus_tag="AC1LW8_002782"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP4774522.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
BASE COUNT          417 a          140 c          150 g          371 t
ORIGIN      
        1 aattatgaat ctttaaactg tgatggtctt tgtaaattaa ttattggatt tgtccattta
       61 caaacaaagt ttgtagataa tacatatacg atgattacag atatacttat taaataaaga
      121 taagtcatta atgatatagg attatcgaat gggtaccatt caaatcctct aacaatgcca
      181 ataattaaac catgtaataa atatacgtat agcgtacgac taccaatata agtatataat
      241 tttttctttg ttgacattaa atttagaaac gcagtcattg cgattaatat aattccatat
      301 aatataagtc gtttaaaagg actgaatata ctctgtcctt cattttcaag tgaagtatat
      361 ggtgaacttc ccaataacca atctgcattg ataggatgaa tcacgtaaac gataaaaaac
      421 aaaataaagg taatgataga tactggtatt agttttttat ttttaaaaat agccgtatgt
      481 tttttggtga aaatgtaacc tagataaaat attgggaaaa atacgattgt ccttgaaatg
      541 cttaagtagc tatcgatgtt atctgaaaaa cctgctccaa tagatataat aattgaaact
      601 gatagcactt tatatggatt aaatcttcta actattacta aaatgacatg aaagaaaaat
      661 agcgtgatca aaaaccataa cgcaaatact gggttaaaag gatcaagttg taattcgtca
      721 cttttacctg ttaagaaata ataaattgaa aagaatgcaa aaaatatcat ataaggtact
      781 atcaaacgtt ttgaaatttt ttctaaatag tatggtttat caatattttt cgcgaaataa
      841 ccagatataa acaaaaatgt tggcatatgg aaactataaa taactaggta taatgctgat
      901 aaatatttat cgccactagt gtagggttgt aacatatgtc caaatacgac taataatatt
      961 aaaattgctc ttgcgttgtc aaaaaaataa tctctctctt ttaatgaagt catattttat
     1021 ctcctttatg tccataagac tcttactaat ttattatatt ttgtattagt tatgcaac
//
LOCUS       DBNENW010000303         1066 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.307, whole genome shotgun sequence.
ACCESSION   DBNENW010000303 DBNENW010000000
VERSION     DBNENW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.307"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..805
                     /locus_tag="AC1LW8_002783"
     CDS             <1..805
                     /locus_tag="AC1LW8_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP4774523.1"
                     /translation="QNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKTIED
                     IEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASILEVQALTGMRIGELQAL
                     QEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSISINSRTVEIFKKIILE
                     NKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRHTHI
                     TLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS"
     gene            complement(848..>1066)
                     /locus_tag="AC1LW8_002784"
     CDS             complement(848..>1066)
                     /locus_tag="AC1LW8_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774524.1"
                     /translation="IKTKLEELDNTLGKWEPFLNDLKAVSPILDNHLDDIVKNMKPLI
                     VQMIFEPSHYDDIFNSRKALTPVFSSVL"
BASE COUNT          438 a          164 c          159 g          305 t
ORIGIN      
        1 tcaaaatcaa gtaacccgtc aactcggaga tatgaaagga gctattaaat atgcagttaa
       61 attttacaat tatccaaatg aatatttgtt aactaatgtc aaaattccta aaagaagaaa
      121 aacaatagag gatatcgaaa aagatgaatc taaaatgtac aactatttag aaatgaacca
      181 agtcctacag atacgtgatc atatactaaa tgataataag ttacacaagc gaaatcgcat
      241 tttaattgcc agcatcttag aagtacaggc tttaactggt atgcgcatag gagaactaca
      301 agcactgcag gaaaaagata tagatttatt aaacaaaact atcaatataa caggtacaat
      361 tcaccgcatt aaatacgagg aaggattcgg atacaaagac actacaaaga ctataagttc
      421 aaaaagaagt atcagcatca attctagaac cgtagaaatt tttaaaaaga taatactgga
      481 aaacaaaatg ttgaaaagat ggaattcgag ctatgttgac agagggttca tattcacaac
      541 aaaaaaaggg aatcctttat gtaataatca aatcgccggt gtgcttaaga aaactacaaa
      601 agctttaaat atgaataaga aagttaccac gcacacattt agacatacac acataacttt
      661 attagtagaa atgaatgttt ctttaaaagc aattatgaaa agggtaggac atgtagatga
      721 aaaaacaacc attcgcatat atactcatgt aactgaaaaa atggatagag aactaactca
      781 aaaactcgaa aacattccaa gttagcttaa atctgccctt tttttgccct tatatttttt
      841 acaagcttta taaaacgctt gagaacactg gcgttaaagc ttttcttgaa ttaaatatat
      901 catcataatg tgatggttca aatatcattt gtacaatcaa aggcttcatg ttcttaacaa
      961 tatcatctaa atggttatct aaaattggtg acactgcttt taaatcatta agaaaaggct
     1021 cccatttgcc taaagtatta tctaattctt ctaatttagt tttaat
//
LOCUS       DBNENW010000304         1058 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.308, whole genome shotgun sequence.
ACCESSION   DBNENW010000304 DBNENW010000000
VERSION     DBNENW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.308"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            454..884
                     /gene="tnpA"
                     /locus_tag="AC1LW8_002785"
                     /pseudo
     CDS             454..884
                     /gene="tnpA"
                     /locus_tag="AC1LW8_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          387 a          151 c          221 g          299 t
ORIGIN      
        1 cggataatta atccgtcttt ttttatgaaa agaataggct gggaaataaa tcaatcttct
       61 atgatctacg aagttatatt ggcagtaatt gactgaacga aaatgcgctt gtaataagct
      121 tttttcaatt ctagtcaggg gccccaacac agagaatttc gaaaagaaat tctacaggca
      181 atgcgagttg gggcggggcc ccaacaaaga gaaattggat tcccaatttc tacagacaat
      241 gcaagttggg gtgggacggc gaaataaatt ttgcgaaaat atcatttctg tcccactccc
      301 cagaaaaggt tctaccattg tcaaaaaaat gcatctctac atgttagagt aaatattggt
      361 cagccaacca aaataatcaa cacgaggaga tgctatttaa tgtcatctga cacaaacagt
      421 ttagcacata cgaaatggaa ttgtaagtaa catattgtct ttgcacctaa atacagaaga
      481 caagcgatat atggaaaaat aaaaaaagat atagggatta tattacgtca attatatgaa
      541 agaaaaggtg tagagataat tgaagcagag gtatgtaaag atcatatcca tatgttagta
      601 agtataccac ccaaacttgg ggtatcatca tttgttggct atttaaaagg aaaagtaatt
      661 taatgatatt tgatagacat gctaacttaa agtatagata tggaaataga aagttttggt
      721 gtaaaggatt ttatgtggat acagtaggta gaaataaaaa agtgattgaa aattatattc
      781 gtaatcaatt acaagaggat atcgttgcag atgaaatctc aatggaagaa tatttagatc
      841 ctttcactgg agagaaaaat aaaaaaagaa agaaaaaaga gtaaaccttt aggattgctg
      901 gaatagtagt gcagttggct gacttgtcag tgccctttta gggctggcca gtaaggaagg
      961 cttatagccg cagaacaaac cacccgttca cacgggtggt ttttatttgt aacatctgtt
     1021 gataggtatc gacaaggacc ttttaatgtt gtgttttg
//
LOCUS       DBNENW010000305         1051 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.309, whole genome shotgun sequence.
ACCESSION   DBNENW010000305 DBNENW010000000
VERSION     DBNENW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.309"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..262)
                     /locus_tag="AC1LW8_002786"
     CDS             complement(<1..262)
                     /locus_tag="AC1LW8_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing sensor histidine kinase AgrC"
                     /protein_id="HIP4774525.1"
                     /translation="MEALNDYNYVLFVIVQVSLMFFISAFISGIRYKKSDYIYIIGIV
                     LSSVYFFDKIGSISLVVITIFIIIFLYFKIRLYSVFLVMVTQI"
     gene            complement(287..427)
                     /gene="agrD"
                     /locus_tag="AC1LW8_002787"
     CDS             complement(287..427)
                     /gene="agrD"
                     /locus_tag="AC1LW8_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide AgrD"
                     /protein_id="HIP4774526.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
     gene            complement(431..994)
                     /locus_tag="AC1LW8_002788"
     CDS             complement(431..994)
                     /locus_tag="AC1LW8_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HIP4774527.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALISVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
BASE COUNT          392 a          152 c          134 g          373 t
ORIGIN      
        1 tgatttgagt aaccattact aaaaatacag aatatagtct aattttaaaa tagagaaaaa
       61 ttattataaa aattgttata acaactagtg aaatacttcc gattttgtca aagaaatata
      121 ctgaagataa cactattcca ataatgtata tataatctga ttttttgtat cttattccac
      181 taataaatgc agatataaag aacattaatg aaacttgaac aattacaaat aaaacgtaat
      241 tataatcatt taatgcttcc acttactatc acactttctc ttatagttat tcgtgtaatt
      301 gagttaattc ttttggtact tcagcttcgt ccaataaaaa atcacaattt atatacgcgg
      361 ctctgtaacc gatgctgttg aaaaagtcaa ctaaaagctc aataactttg ttgagtaatt
      421 ttttcataat ttagtcctcc tttgaatagt atattggtag taatgtaata gcttgtataa
      481 taatacccag ttgaataaat tgggtatatg gttcttttac aaaaagtgtg ataatgaaaa
      541 gaattgtact aattataatt gaaaaatatc ttttttgctt tacaagcctc gctgggatag
      601 gcttcttctt agtggctgca ggtgcatatt taatcactac tccaacactt attaatgcta
      661 aaaacatcat tagtgtttca ttaatatgaa aatgcaatac taatagaggt agcacaataa
      721 aaagtgtaat gctttctata taacaccaaa aagatgaagg tgcatgtgca ccatgtgcat
      781 accttctaat tagataaaac gatatattag taattaaagt gaagataaaa atgtttaaaa
      841 tataggcaat agtatacata acaattaatt tacctatatt tttagctaag acctgcatcc
      901 ctaatcgtac ttgcaaaaat tgaatatgat ctaagttatt tctcttttga agatacgtgg
      961 caaactggtc aattttatta tcaaaataat tcaattttac accactctcc tcactgtcat
     1021 tatacgattt agtacaatct tttatcatta t
//
LOCUS       DBNENW010000306         1047 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.310, whole genome shotgun sequence.
ACCESSION   DBNENW010000306 DBNENW010000000
VERSION     DBNENW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.310"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            97..894
                     /locus_tag="AC1LW8_002789"
     CDS             97..894
                     /locus_tag="AC1LW8_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP4774528.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNSSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          355 a          188 c          174 g          330 t
ORIGIN      
        1 tggaaatcgt aatataaaac gaaaacttaa tttactatat aaattgtctt aataattttt
       61 aaaagtagta aaacttaatt ttaaggagga gtccctttga aaaaattagc atttgcaata
      121 acagcaacat ctggtgcagc tgcattttta acgcatcatg atgcacaagc ttctacacaa
      181 catacagtac aatctggtga atcattatgg agtattgctc aaaaatacaa cacttcagta
      241 gagagtatta aacaaaataa ccaattagat aacaacttgg tattccctgg tcaagttatc
      301 tcagtaggtg gaagtgatgc acaaaattcg tcaaacactt ctccacaagc tggttcagca
      361 tcatctcata ctgtacaagc tggtgaatca ttaaatatca ttgctagcag atatggtgtt
      421 tcagttgatc aattaatggc agccaataac ttacgtggtt atttaattat gcctaaccaa
      481 acattacaaa ttcctaatgg tggatcaggt ggtacaacac caacagctac aacaggtagc
      541 aatggcaatg catcatcttt taatcaccaa aatttataca ctgctggtca atgtacatgg
      601 tacgtatttg accgtcgtgc tcaagctggt agtccaatta gcacatattg gtcagacgct
      661 aagtattggg ctggtaacgc agctaatgat ggttaccaag taaacaacac accatcagtt
      721 ggttcaatta tgcaaagcac acctggccca tatggtcatg ttgcttatgt tgaacgtgtc
      781 aatggtgatg gtagtatctt gatttctgaa atgaattaca catatggtcc atacaatatg
      841 aactaccgta caattccagc ttcagaagtt tctagctatg cattcatcca ttaattaaat
      901 aaattgtact gatatatact agcaattcac atcatgtgag attgctagtt ttttattttt
      961 gaaaaaaatt ttcattttgg tacaaaaaat tatctcaccc ttccctatca tacatattta
     1021 tattttgtat gaatggtagt taggtaa
//
LOCUS       DBNENW010000307         1038 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.311, whole genome shotgun sequence.
ACCESSION   DBNENW010000307 DBNENW010000000
VERSION     DBNENW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.311"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            26..157
                     /locus_tag="AC1LW8_002790"
     CDS             26..157
                     /locus_tag="AC1LW8_002790"
                     /inference="COORDINATES: protein motif:HMM:NF013303.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774529.1"
                     /translation="MFGVHFGANCVGEAGENSACLYGGVTFHEKMKNNKKVNRHKCI"
     gene            186..452
                     /locus_tag="AC1LW8_002791"
     CDS             186..452
                     /locus_tag="AC1LW8_002791"
                     /inference="COORDINATES: protein motif:HMM:NF014875.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin beta-grasp domain-containing protein"
                     /protein_id="HIP4774530.1"
                     /translation="MTTAKIKPTAQELDLKTRNNIQNQYKMYDSETGDIQKGYIKFHS
                     TKSSFYYDLFDFKVKKRVDFLKFYNDNELISTKKLHIDIYLFNK"
     gene            593..>1038
                     /locus_tag="AC1LW8_002792"
     CDS             593..>1038
                     /locus_tag="AC1LW8_002792"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP4774531.1"
                     /translation="MEYKSYNKMSNNILSILLSDKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIAGLLTYKVYDEYIEIISLDSFVENKGIGSHLLDYAEIIASDMSKRSISVITT
                     NENIKALYFYQKNKYRITDVIFDAVTEARKIKPSIPTIDKNGIEIR"
BASE COUNT          408 a          122 c          179 g          329 t
ORIGIN      
        1 acacattcaa aggaaaaatg tagatatatt cggagtacac tttggagcaa attgtgttgg
       61 agaagcaggt gagaatagcg cttgtttata tggtggagtc acatttcacg aaaaaatgaa
      121 aaacaacaaa aaagtcaata ggcataaatg tatttaaaaa tggatttcaa caaaaagggt
      181 ttgtcattac tactgctaaa atcaaaccca cagctcaaga gctagatctt aaaacgagaa
      241 acaatataca aaatcaatat aaaatgtatg attcggaaac tggagatata caaaaaggat
      301 atataaaatt tcattctact aaatcaagtt tctattatga cttatttgat tttaaggtga
      361 aaaaacgtgt ggatttctta aagttctata atgacaatga acttataagt accaaaaaat
      421 tacatataga tatctatttg tttaacaagt aaataattgg aaacgtttat tgatacctta
      481 cagccatatg ggcagacaaa atgtcagttt atcttttcat cagttgtgta aattactttg
      541 caaaaactta gtaagtgtgg gaaagattat taacagagag aagtgactta ttttggaata
      601 taaatcttac aacaaaatga gtaataatat tttatctatc ttgttgagtg ataaaaattt
      661 cgataataaa atggtagtct caacaggtag ttacattttg tctgagttgc catgtattgt
      721 tgcctataaa aataatgata tcgcggggct actaacgtat aaagtttatg atgagtatat
      781 tgaaatcatt tctttagata gttttgtaga gaacaagggt attggtagtc atttgcttga
      841 ttatgctgaa ataattgcct cagatatgag taaaagaagt atcagtgtta ttactacaaa
      901 tgaaaatatt aaggcacttt atttctatca aaaaaataaa tatagaatca cagacgttat
      961 ttttgatgct gtaacagaag ctaggaaaat aaagccgtcg attcctacaa ttgataaaaa
     1021 tggcattgaa attagaga
//
LOCUS       DBNENW010000308         1024 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.312, whole genome shotgun sequence.
ACCESSION   DBNENW010000308 DBNENW010000000
VERSION     DBNENW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.312"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            3..887
                     /gene="hlgA"
                     /locus_tag="AC1LW8_002793"
     CDS             3..887
                     /gene="hlgA"
                     /locus_tag="AC1LW8_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HIP4774532.1"
                     /translation="MAPLANPFIEISKAENKIEDIGQGAEIIKRTQDITSKRLAITQN
                     IQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSN
                     VDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEV
                     ESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSG
                     FNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVK
                     YEVNWKTHEVKIKSITPK"
BASE COUNT          408 a          173 c          153 g          290 t
ORIGIN      
        1 taatagcccc tttagccaat ccatttatag aaatttctaa agcagaaaat aagatagaag
       61 atatcggcca aggtgcagaa atcatcaaaa gaacacaaga cattactagc aaacgattag
      121 ctataactca aaacattcaa tttgattttg taaaagataa aaaatataac aaagatgccc
      181 tagttgttaa gatgcaaggc ttcatcagct ctagaacaac atattcagac ttaaaaaaat
      241 atccatatat taaaagaatg atatggccat ttcaatataa tatcagtttg aaaacgaaag
      301 actctaatgt tgatttaatc aattatcttc ctaaaaataa aattgattca gcagatgtta
      361 gtcagaaatt aggctataat atcggcggaa acttccaatc agcgccatca atcggaggca
      421 gtggctcatt caactactct aaaacaatta gttataatca aaaaaactat gttactgaag
      481 tagaaagtca gaactctaaa ggtgttaaat ggggagttaa agcaaattca ttcgttacac
      541 cgaatggtca agtatctgca tatgatcaat acttatttgc acaagaccca actggtccag
      601 cagcaagaga ctatttcgtc ccagataatc aattacctcc tttaattcaa agtggcttta
      661 atccatcatt tattacaaca ttgtcacacg aaagaggtaa aggtgataaa agcgagtttg
      721 aaatcactta cggcagaaac atggatgcta catatgctta cgtgacaaga catcgtttag
      781 ccgttgatag aaaacatgat gcttttaaaa accgaaacgt tacagttaaa tatgaagtga
      841 actggaaaac acatgaagta aaaattaaaa gcatcacacc taagtaaaca gttcaatcat
      901 tttataaaat cctgggacac ttcatacttg tctcaggatt ttttaacata ttgaatcagc
      961 ctcataacat taaattaatt tatcgtatat taaatttaat aataacaact gatttttata
     1021 agaa
//
LOCUS       DBNENW010000309         1003 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.313, whole genome shotgun sequence.
ACCESSION   DBNENW010000309 DBNENW010000000
VERSION     DBNENW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.313"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(148..>1003)
                     /locus_tag="AC1LW8_002794"
     CDS             complement(148..>1003)
                     /locus_tag="AC1LW8_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIP4774533.1"
                     /translation="ENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADALNNM
                     TDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQNAPRL
                     FKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSLVSIG
                     TAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAYRNDI
                     LEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGISSVY
                     VHPEPDHL"
BASE COUNT          359 a          183 c          141 g          320 t
ORIGIN      
        1 caataaccac ctaataacaa atcatattat acacctttgt tcgctatttt tctaaggttt
       61 aaaaaatatt tttaggtaag cctaaaaata gatgtaataa aaacgcctcc tcagatattt
      121 atatatctat gaagacgttt aaatacatta tagatggtct ggttctgggt gaacgtatac
      181 tgaggaaata ccttttttgt gcaaatgatg ttcgacattg tcacaaattt gatgcgcttc
      241 tactaaggaa aggttagcat ctacaacaat tgtgacatca ataaacacac tacttccatg
      301 gtaacgccct ttaatacttt taacttcttg tacttcatca acttctaaaa tatcattgcg
      361 atacgcttct aattcagttt cattgaaacc atcactcaac ataaaaattg cttctttaaa
      421 aataccaaaa ccagtataaa cgattagtaa gcctagtaat gttgctaaaa taatatcgac
      481 aattgggaaa ccgatttgcg taaaaattaa tcctatcgct gttccaatgc tgactaaact
      541 atctgataaa ttatctttgg cagccgaatt taaagaacta cttttcgttc ttttcgctag
      601 tctttgattg actgcaaata caatcaacat tacaagacca ctgattaagc tgacgataat
      661 tgttattgcg ttaggtacaa cgtcatcttc tttgaacaaa cgaggtgcat tttgaataac
      721 tacttggata cctacaaaca taatgacaaa tgacaccaat aatgaagaaa tattttcaga
      781 cttcaaatgg ccataaggat gatttcgatc tgcaggttta attgaaattt tcaatccaat
      841 aataacagct aaagaaacga taatatctgt catattgttt aatgcgtcgg ctcttacagc
      901 tgcagagtta aagacaaaac ccgtgacata cttaacaata gataagatta tatatacaat
      961 taaactcaaa taagcaccgc gttgcgccaa tttaagattt tca
//
LOCUS       DBNENW010000310         1001 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.314, whole genome shotgun sequence.
ACCESSION   DBNENW010000310 DBNENW010000000
VERSION     DBNENW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.314"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..360)
                     /locus_tag="AC1LW8_002795"
     CDS             complement(<1..360)
                     /locus_tag="AC1LW8_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774534.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLN"
     gene            complement(813..>1001)
                     /locus_tag="AC1LW8_002796"
     CDS             complement(813..>1001)
                     /locus_tag="AC1LW8_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774535.1"
                     /translation="LIVFAYFFLGSASIFTLTILLTTESQNIIIFLTWVIISMFFFLV
                     NMASIGNKNVHVILRINE"
BASE COUNT          369 a          180 c          105 g          347 t
ORIGIN      
        1 atttaagtta aaataaaaaa ctataacaca aattaaagcg agagcacaca atatcattac
       61 aactagctta tctaattgaa catcaaaaac atgtatgtat ttccttaaag taactccaaa
      121 caaaaccgaa cttcctataa ctgaccagaa aacattattt ttatttggtt taacaacatt
      181 taaattttca aattcttctt cgctaattat cgcgtacttt tttggaatga accaattaat
      241 gaaaggaaaa aagtacacga accaagtgct tactaaatcg actaataagt attcaccgtt
      301 atgttgaatg actttgtatt tcggattttt ataaatgact ctgacatcgc aaagcaacat
      361 ttaccactcc cgtttcatta gttttaaatg atccccaata ctttttgtac cttcaccaac
      421 tatcgttcca acactatcta cagctttttc tgtggtattt ttaaacagcg tctatttaat
      481 gtcttatcca attagtttta tctcttaatc caaatttatt agtatgaaaa gtccaatttt
      541 taagaattgt ctaaacagat tccgcaacaa aaccagctaa aaccgtccat actattggat
      601 taaaaacgcc aatgttataa gagctatcat acaaaataac acgctctata tttcattttt
      661 aaattaaaac agttttttcc tttatcaatc acaatgggca ttttcacttg gtatattcaa
      721 acattactat ctattttcct gatttaagat atgattatat ttttttgaac caccgtttaa
      781 atatgcgata ctaccaattt cgatcatttt tactactcgt ttattcttaa aataacatga
      841 acatttttat tacctatcga agccatattc actaaaaaga aaaacatcga gataataacc
      901 caagttaaaa atattattat attttgtgac tctgtcgtca aaagaatggt taaagtaaat
      961 attgaagcgc ttcctaaaaa aaagtacgca aaaactatta a
//
LOCUS       DBNENW010000311          982 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.315, whole genome shotgun sequence.
ACCESSION   DBNENW010000311 DBNENW010000000
VERSION     DBNENW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.315"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>982
                     /locus_tag="AC1LW8_002797"
     CDS             <1..>982
                     /locus_tag="AC1LW8_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HIP4774536.1"
                     /translation="EFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKL
                     ELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDEN
                     HIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNE
                     GEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFS
                     KEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAEL
                     FTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEES"
BASE COUNT          403 a          104 c          146 g          329 t
ORIGIN      
        1 aagagtttga agaaaagaaa aaatacttaa gtgaaattta tgaattgata gacgaaatta
       61 atattgaccc ttatattttt ctgacagaac ataatgaatg taatatatac aaaataagtg
      121 agaatgaaaa attagaactt caaacttata tgagtaaaat cgaaaaagta ttattaaaat
      181 atcaaacgta tttaaaagaa acatgtaaag aatgcttgtt ttatccttat ttgctagttc
      241 aaggtgaagc tggcatagga aaatcacacc ttttagcgca tctatctaaa aaacttagag
      301 atgaaaatca tattatttat ctgtttttgg gacaattttt tactaaaaat gaagatccat
      361 ggcatcaaat acttaatgat ttagaagtta caaattctgt tgataatttt ttaaggtcaa
      421 taagtaataa ggccaaagaa acaaaaaaaa gagcttttat tattattgat gcgcttaatg
      481 aaggtgaagg taaaaggtta tggggaaatt attttcaaag ctttatcaac catataaaga
      541 agtattctaa tatagcttta atattttcta ttagaacccc ttttgaagat gttatattac
      601 ccaaaaacgc aatacaagat aacaatattg tagtatttca gcatgaaggt tttagtaagg
      661 aagaaaacta taatccaatt gtatcttttt gtgattttta tggattagag ctacctaagt
      721 tacctatatt aaatccagaa ttcaacaatc cattattttt aaaactaatg tgtgagtact
      781 gtgtaaataa atttaaggaa tttgaccaaa ctataagtgt tgcagagcta tttacaaatg
      841 ttcttaagac agtaaatatt aatttatcta aggaagataa atttgatttt gataaaaata
      901 ttaatgttgt acaaaaagta ataaaaggat tagttgaatt aatgaacgac tcagaattta
      961 atcaactaaa ttatgaggaa tc
//
LOCUS       DBNENW010000312          982 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.316, whole genome shotgun sequence.
ACCESSION   DBNENW010000312 DBNENW010000000
VERSION     DBNENW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.316"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            13..708
                     /locus_tag="AC1LW8_002798"
     CDS             13..708
                     /locus_tag="AC1LW8_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4774537.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            710..>982
                     /locus_tag="AC1LW8_002799"
     CDS             710..>982
                     /locus_tag="AC1LW8_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4774538.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNKIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRT"
BASE COUNT          378 a          134 c          160 g          310 t
ORIGIN      
        1 ggagatagtg atatggatgt tttaacaata gaacatttaa caaagaagat aggcaacaaa
       61 acgattctcg aagatgtatc atttaagctg aaacgcggac aaatagttgg tctcgttgga
      121 gcgaatggtg caggtaaaac aactttaatg aaagttatat taggttactc tagtttccaa
      181 agcgggaatt ttaatgttat taacagcaaa gacagcaaaa gcaatatcgg tgcattgatt
      241 gaaaatccag gaatatatcc ttttatgtct ggatatgaaa acttgaagtt attgaatgaa
      301 tcaaaaaaca ctcaagatat cgataaaatt gtctcacaac ttcatatgga tgaatacatt
      361 cataaaaaag ctaaaacgta ttctcttggt atgaaacaaa aattaggaat tgctatagca
      421 tttttaaatg aacctcaatt tattatctta gatgaaccaa tgaatggctt agatccaaaa
      481 gctgtgcgag atgttcgtga attgattgtc caaaaagcgc aagaaggtgt tactttctta
      541 atttcgagtc atattttaag tgaattagtt aaaatcacaa actctatcct tattattaac
      601 aaaggtaaaa ttgttacaga aacatcggaa gaagaactta aacaatttaa agataatgat
      661 ttagaaaatg tattactaga aatcatagaa agggaggacc aagcataaaa tgggaacttt
      721 aattaaacaa gaatgtttca aattatttaa aaagaaatca acttttatcg cacctattgt
      781 ctttattcta ctaatggttg ctcaaggtta tattgctaca aaatacaata aaatttttac
      841 gccacaggaa tctttcacat ctgcttataa tggtttttca tggtttgcat ttttattaat
      901 tattcaagca agtacaatca tttcaatgga atttcattac ggtacgatta aaaatttact
      961 ctatcgtgaa tattcaagaa ca
//
LOCUS       DBNENW010000313          965 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.317, whole genome shotgun sequence.
ACCESSION   DBNENW010000313 DBNENW010000000
VERSION     DBNENW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.317"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(1..817)
                     /locus_tag="AC1LW8_002800"
                     /pseudo
     CDS             complement(1..817)
                     /locus_tag="AC1LW8_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958488.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT          339 a          178 c          108 g          340 t
ORIGIN      
        1 cttaaagata ttttcattaa atctgtcaaa tcaatagacg ctatattttt caacttatct
       61 ctatatttat ttttagtacg tctttctaaa tttccccatt cctcttcttc gtgagttaat
      121 aaatgaagca ttgctcgttc ttgtatattt tcaatcattt ttaaattcgg ttttaaaata
      181 tgcaaatcat caaaacaatc tttccaacaa tcaaccatat ctcgttttaa ttcaatttcc
      241 acacgccata gaaatgttga atcaatttca acatctgcat tatctttacg ttcttgtttt
      301 tattataaat ccgaataaac ctatcactat tacgcacacc aaaatatttt gtttctggtt
      361 ttacattacg tccataaaat atagttttct ttgccgactt atctgacaat gcataatagt
      421 catttaaatc aaattcaaaa tcaaaagcca aatctaatct cgtaaaacta acatcgtcca
      481 aataactgat gatattttgt tttaaccaaa gcacttcatc atgcgaaagc ttattaggat
      541 taaattcaac gcgcatacta cgtctattcc aaagagttgc ttttattttg tcatattcaa
      601 tataaacttt ttctttaaga gctttagctt taaagtttgt ttgtaaaata tcccaaagcc
      661 gaacttcagg attagtactc ataaaatgtg aaagtctctc tgcgttagac atgctaagat
      721 tcccaacaat cgttatagcg tcaaaagaca attttggaat agctagtgac atcctatgtc
      781 aatttaaccg gctattaccg gatattagag tatccagttt tacacatgga tgaaacgaaa
      841 ttcaaaaccc taaaaaatat gttccactaa cagcaaaaaa ataccattat gttcctacta
      901 aaaaactaaa aatactggag aacaaatgtc aggatataac ttaggatact atgtaataaa
      961 aattt
//
LOCUS       DBNENW010000314          961 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.318, whole genome shotgun sequence.
ACCESSION   DBNENW010000314 DBNENW010000000
VERSION     DBNENW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.318"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..755
                     /locus_tag="AC1LW8_002801"
     CDS             <1..755
                     /locus_tag="AC1LW8_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIP4774539.1"
                     /translation="YETKSFTRLQIMSFFSSLWISSYSIELDENKNTFSIKQTKSLKK
                     NQQVILSNIIIYAIYDVIRKYWSGTGDRKLMDIYISQNNRYLKPLAKEVFRNELLRWN
                     EENINKTSINIQTDEKMIITFIANKFRSFYSSLNDNLDYEHIFSRKLYKLHKGNAIIP
                     AGSLGNIMLLDSGINRRKKEKFLYSAIDIDKASITGTIENQYVNNSFYPDKRTIDTIE
                     IDIIDEKYNSLIKMIKNRGDKLIEEVVRELYK"
BASE COUNT          375 a          100 c          151 g          335 t
ORIGIN      
        1 aatatgaaac taaatctttt actagattac aaataatgtc attcttttca tctttatgga
       61 tctcatcgta tagtattgaa ttagatgaaa ataaaaacac attttctatt aaacaaacta
      121 aatctttgaa aaaaaatcaa caagtgattt tgtctaatat cataatttat gcaatttatg
      181 atgtgataag aaagtactgg tcaggaacag gtgatcgaaa attaatggat atatacatat
      241 ctcaaaacaa tagatattta aagccgttag caaaagaagt gtttagaaat gagctactta
      301 gatggaatga agagaatata aataagacaa gtattaatat tcaaacggat gagaagatga
      361 tcattacttt tattgctaat aaatttagaa gcttctattc atctttgaat gataatttag
      421 attatgaaca tattttttca agaaaattat acaagctgca taaaggtaat gcaataatcc
      481 ctgctggatc tttaggtaac atcatgttac ttgactctgg tattaataga agaaaaaaag
      541 aaaaatttct ttattcagca atagatattg ataaggcatc tattacggga actatagaaa
      601 atcagtatgt taacaacagt ttttatccag ataaaagaac aatagatact attgaaatag
      661 atataattga tgaaaaatat aatagtttaa taaaaatgat caaaaatcgt ggtgataaat
      721 taatagaaga agttgttaga gaattatata aataagtttg atatatcttg ctttttatta
      781 tcacgctagt tgatggtatc tttaaagtgt gtattacctg attgatatca ataccaggta
      841 tcgaatgaat atttttaaaa taattgagtt gttgtgctaa ttgaatcatg gcatcattta
      901 attgtttgat acgatggata gattgtttta attgtttagg cggcctttgt tggtcgccat
      961 t
//
LOCUS       DBNENW010000315          959 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.319, whole genome shotgun sequence.
ACCESSION   DBNENW010000315 DBNENW010000000
VERSION     DBNENW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.319"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(153..845)
                     /locus_tag="AC1LW8_002802"
     CDS             complement(153..845)
                     /locus_tag="AC1LW8_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIP4774540.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
BASE COUNT          295 a          192 c          133 g          339 t
ORIGIN      
        1 atatatgtgt aattctaaaa gattcttctt taaaaatata aataccacga catattgctt
       61 taacattttc atttataaag cgaaaaaatg catcgctact aagttgaatg tttagtaagg
      121 atgcattgaa ttcactaaaa tgattaaatt acttatatct tttcatctga ttgattatcg
      181 aaatttcttc cttctaaacc tgctaactct tctttagaag ctgcaggtgc tttcatttca
      241 aatatctcat tcactactgt gtaatcgtaa tatcctaatc tggcaatagg tttaatcgac
      301 ttaatgtcca atttaccatt atcaagaata accttatcgt caatatgaac ttgagcaact
      361 cttcctataa caatatctac ggtagatact ggatctccag ttggaatacg aatcgtttga
      421 acgtactcac attcaaaatg aactggcgat tcttttacac gatatcctgg agcttctata
      481 catttttcct ttgttacacc tgcaaaatta aattcatcct cttctggtgg caatgctttc
      541 gatgataaat taactgcttc tcttaaatca tacgttgcca tattccacac aaaccaacct
      601 gtctcttcag catttttcac tgtatcttta cgttcgtgat caccaagaac ggattgattt
      661 gctgcgaaca taaccatagg cggatcccaa gttaagtttt gatactgact ataaggcgct
      721 aaattatctt tcccatcttt cgatacagta gagatccacc ctattggacg tggtactgta
      781 ctacttttaa atgggtcgtg cggtaaacca tgacttctta caccttgttt tggcgaataa
      841 ttcatactat cttcacccct tataagtaat tacatttaag gttacgccct ctttacataa
      901 gcgtctaata taaataaaca atttatttat aagtagaaac tatatatgac gtggttgct
//
LOCUS       DBNENW010000316          892 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.320, whole genome shotgun sequence.
ACCESSION   DBNENW010000316 DBNENW010000000
VERSION     DBNENW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.320"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..659)
                     /locus_tag="AC1LW8_002803"
     CDS             complement(<1..659)
                     /locus_tag="AC1LW8_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HIP4774541.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     V"
     gene            complement(724..867)
                     /locus_tag="AC1LW8_002804"
     CDS             complement(724..867)
                     /locus_tag="AC1LW8_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HIP4774542.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
BASE COUNT          297 a          142 c          109 g          344 t
ORIGIN      
        1 tatactttca ctttagtaat atcattaatt tcaaattctt gatttatctt ttcagctaaa
       61 tttgaaaaag taatattatt attcgtcaca gtggttttga tgaatacgag taattcgcta
      121 tttttaatgg aaaaagatgt atctttattg ttgtttaaat aaatagcact ttgttcattt
      181 aaatatattc gatcattgat aatatgtgct ttactattcc aaataacttt caaattttga
      241 taatattcat caatactctc aatataacta accagtttat acaaccattc gccatcgact
      301 ttcacatatt tttgaatact atttccaact ttaagacgtg ttggttcatt tacaaaatgc
      361 cccaaattaa taccacttaa taatccaaat ggagttggac gtgttgacat tctaattaaa
      421 tattttaata aactttcttt agcatctcta accttcttgt tatcgccatc aaaatttatt
      481 tcttgtaaag tacaatataa gttaaatgtt gttgttagga ttgattcttt catctgagca
      541 tttaattgta agtcgctata tttaatttgt tcagtattta aaaataaatt gaaaaattca
      601 actgataata aaggtgttct aatcatacaa atacttgacg gttcgtaaat agtgttcatt
      661 tgtaagcacc tcacatattt gaatagacaa atagagccct ttcaggctct atttgctgta
      721 atcttaacag cagaagctat taaaactacc agtcttagca caaccaggag tacataaact
      781 atgacttgta atacgttcgt cacctgctga atcatttgag ttattgtttg ctttaacttg
      841 cacgtctaaa tcaagaactt tttccataga taaaacctcc tattattagt tg
//
LOCUS       DBNENW010000317          887 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.321, whole genome shotgun sequence.
ACCESSION   DBNENW010000317 DBNENW010000000
VERSION     DBNENW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.321"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            7..>887
                     /locus_tag="AC1LW8_002805"
     CDS             7..>887
                     /locus_tag="AC1LW8_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP4774543.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDMILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNY"
BASE COUNT          360 a          112 c          128 g          287 t
ORIGIN      
        1 atagaggtga ttaaattgat tcaaatatct aatatcaaca agtcatttaa taaaagatgt
       61 gttctaaaaa atatttcgtt cgatattgaa caaggtaaat gtatcgcttt aattggaaaa
      121 aatggtgctg gaaagtcaac gttaattgat atattaattg gtaatgttaa tgctaattct
      181 ggtgagatat ttgataaaga caagttatta caaagtgaaa atcgcagtat aatgttccaa
      241 aaaacgatgt ttccagatca attaaaagtt attgagatta tcaacttata tcaatcattt
      301 tacgaaaatc cattaccatt ggaagaaata atagaactga cgaaatttga ttctagtcaa
      361 ctgaaccaat ttgtaaataa actttctggt ggtcaacaac gattactcga ttttgtatta
      421 tctttaatcg gacaaccaca attgatctta ttagatgaac caacatcgac tatggatata
      481 gaaattagag aatatttttg gtcaattatt gaaaatttaa aagaagataa tcgaacgata
      541 ctctatacat cgcactatat tgaagaagtc gaacgtatgt cagacaaaat tattctcatt
      601 gaaaatggag aaataatact taatgattca acgtcacata ttagaaccaa tcagcaatct
      661 cagattacgt tatccgatga atatataaga aagttaaaac tagataaaga tgatttagtt
      721 attcaaaaaa atcataatgg cactatcaaa attattactt caaatgtaaa tgatatgatt
      781 ttatatcttc aacaacttca tattaatttg gatgatattg aaatacaaaa agtctcaatt
      841 gttgattcat acttcaacaa taaaaagcaa aggggatcta attatga
//
LOCUS       DBNENW010000318          882 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.322, whole genome shotgun sequence.
ACCESSION   DBNENW010000318 DBNENW010000000
VERSION     DBNENW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.322"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            3..713
                     /gene="sarS"
                     /locus_tag="AC1LW8_002806"
     CDS             3..713
                     /gene="sarS"
                     /locus_tag="AC1LW8_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HIP4774544.1"
                     /translation="MEAYMFRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVS
                     DLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIK
                     QFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNI
                     VLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLL
                     AQVNQLLADKDHLHLVFE"
BASE COUNT          346 a          140 c          110 g          286 t
ORIGIN      
        1 tcattgaagc atatatgttt cgttttaaga aaaaagtcaa gcctgaagtc gatatgacta
       61 taaaagaatt tatattactg acttatttat ttcatcagca agaaaacaca cttccattta
      121 agaagattgt ttcagattta tgttataaac aatcggattt agtacagcat ataaaagtac
      181 ttgtgaaaca ttcatatatt agtaaagttc gaagtaaaat tgatgagcgt aatacttaca
      241 tttcaatatc tgaagaacaa cgagagaaaa ttgcagaacg tgttacattg tttgatcaaa
      301 tcattaaaca atttaacctt gcagatcaaa gtgaatcaca gatgatacca aaagatagta
      361 aagaatttct aaacttgatg atgtatacaa tgtatttcaa gaatattatc aaaaaacatc
      421 taacattaag ttttgtagaa ttcacaattc tagctattat cacttctcaa aataaaaaca
      481 tcgttcttct taaagattta attgaaacaa tccaccataa ataccctcaa actgttagag
      541 ctctcaataa tttaaaaaag caaggctatc taataaaaga acgctcaact gaagatgaaa
      601 gaaaaatttt aattcatatg gatgacgcgc agcaagacca tgctgaacaa ttattagctc
      661 aagtgaatca attattagca gataaagatc atttacatct tgtttttgaa taatatctct
      721 attacgcaag tgtgctgtat tctaaagtgc acttgtgttt tctatttttt aataaaacct
      781 cagcacataa tgaacaactt tctattttct atatcactta aaaccatttc cgaaattaaa
      841 cctcagcaca ttcaaagccc cactttattc ttaaaaatat tt
//
LOCUS       DBNENW010000319          871 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.323, whole genome shotgun sequence.
ACCESSION   DBNENW010000319 DBNENW010000000
VERSION     DBNENW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.323"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            3..857
                     /locus_tag="AC1LW8_002807"
     CDS             3..857
                     /locus_tag="AC1LW8_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774545.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT          334 a          119 c          139 g          279 t
ORIGIN      
        1 aaatgtcttt ttatgttgta cttatcatta tcattgtagc attaattggt atattagttt
       61 taaatcaaag gtatagtaat agtaaaatag ataccgaagt ttatgcaaga aagcaactta
      121 ttaaaaagaa caaagcatta agtgcagaaa atgctgaatt aagaagtcaa atgctgagtt
      181 ctaataatga tgttggccac catgcataca aaaatgctaa aagagaatta cgcaagattt
      241 tagatagcta tctcgaaaat ggtaaattaa aatattacga tattatcgtt acaagcaatt
      301 tagctactaa acatccgttt ttcgagtatg cacgttcatt tgattttatc attgtttcag
      361 atatcggttt aataaacgtt gatgtaaaaa gctggggtga aaaaacattt tatcactttg
      421 atgtacctga tgagcatgat acagaaataa gcaatagtaa tattgaaaaa gttgtcggtc
      481 attacattag ccagcaatat catgatcagt ttaattcatc aagaaagtct atctatactt
      541 ttacagaaac agttcaacca aatcgtgtta tttatgattt ttatgattat gatccgtatc
      601 aattagcagc aaataatgcg aaagcattaa aggatcatat tgaacaaaac tttaacttta
      661 aagtccaaag taccggtgtt atttacttta gtgatggtac agttaatatc attcaaggtt
      721 ctgaagaaag agataaatat gttgatacag tttcaacaaa atcttcgtta cgacgtatta
      781 ttagcgaagc catagagcta tctaaacacc ctttaaataa agaacaggtt gatcaaatca
      841 cagctatatt caagtaaaat caaaggcata a
//
LOCUS       DBNENW010000320          831 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.324, whole genome shotgun sequence.
ACCESSION   DBNENW010000320 DBNENW010000000
VERSION     DBNENW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.324"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            115..642
                     /gene="isaB"
                     /locus_tag="AC1LW8_002808"
     CDS             115..642
                     /gene="isaB"
                     /locus_tag="AC1LW8_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HIP4774546.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          331 a           89 c          149 g          262 t
ORIGIN      
        1 agtataacaa aactgttgtg tatttataaa tataatcatt agtattttta tggctgaaaa
       61 agttataata aaagtgtaag gaataaaata tttgtatgga aaagagagat aattatgaat
      121 aaaaccagta aagtttgtgt agcagcaaca ttagcattgg gcacactgat tggagtaaca
      181 gttgttgaaa attcggcgcc aacaagtaaa caggcacagg cagcaataac cccatattat
      241 acttataatg gttatattgg taataatgct aattttattc tggataagaa ttttattaac
      301 gcgattaagt atgataatgt gaaattcaat ggtattaaat tagctaaaac gaatacgata
      361 aaaaaagtag agaaatatga tcaaactttt aaaggtgttt ctgcaaaagg aaacgaagca
      421 agtcaattgc aatttgtagt taaaaataat atttcattaa aagatatcca aaaagcttat
      481 ggcaaggact tgaaaaaaga aaatggtaaa acaaaggaag ctgatagcgg tattttttac
      541 tatcaaaatg ctaaaaagac attaggcatc tggtttgtcg ttgatcataa tagagttgtc
      601 gaagtaacag ttggacatac accatacaaa acaagtaaat aaaataatgg catattaagg
      661 ctagagtgtg aggggagata ccgcattcta gtctttttta ttaaataata acgattattg
      721 cgtcttacat agttgtttga aattgataaa aatgtttatt tgatcatgat tatttaaata
      781 gttatctcac atatttcaag cattgattaa gtgtatagag cactattttg t
//
LOCUS       DBNENW010000321          831 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.325, whole genome shotgun sequence.
ACCESSION   DBNENW010000321 DBNENW010000000
VERSION     DBNENW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.325"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>831
                     /locus_tag="AC1LW8_002809"
     CDS             <1..>831
                     /locus_tag="AC1LW8_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIP4774547.1"
                     /translation="FILTACSASVDKTSNSTKTTDYKIENGETLKVPEKPKRVAVLTG
                     FYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAKAKPDLIVVDAMD
                     KNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEWDDKTRKDKKEIQ
                     SKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKLQEDKKGYASI
                     SKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDPITL
                     "
BASE COUNT          356 a           99 c          143 g          233 t
ORIGIN      
        1 atttattttg actgcgtgtt cagcatctgt cgataaaaca agtaactcga caaagactac
       61 agactataaa attgaaaatg gtgaaacact gaaagtacca gagaaaccta aaagagttgc
      121 tgtattaact ggattttatg ttggtgattt tataaagtta ggaatcaaac caattgctgt
      181 ttcagatata actaaagatt cttcaatttt aaaaccttat ttaaaagggg ttgattatat
      241 tggagaaaat gatgttgaaa gagttgctaa agcaaaacca gatttaattg ttgtagatgc
      301 tatggataaa aatataaaaa aataccaaaa aatagcgcca acaatcccat atacatacaa
      361 taaatacaat cataaagaaa tattaaaaga aataggcaag ttgactaata atgaagataa
      421 agctaaaaaa tggattgaag agtgggacga taaaactaga aaagataaaa aagaaattca
      481 aagtaaaatt ggtcaagcaa cagcatctgt gtttgaacca gatgaaaagc aaatatacat
      541 atataactct acatggggtc gtggtttgga tattgttcat gatgcattcg gtatgccaat
      601 gacaaagcaa tataaagata aattacaaga agataaaaaa ggctatgctt ccatttcaaa
      661 agaaaatatt agtaaatatg ctggtgatta tatattttta agtaaacctt cgtacggaaa
      721 atttgatttt gaaaaaacac atacatggca gaatattgaa gctgtaaaaa aaggacatgt
      781 aatttcatat aaagcagaag attattggtt cacagatcct attacattag a
//
LOCUS       DBNENW010000322          823 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.326, whole genome shotgun sequence.
ACCESSION   DBNENW010000322 DBNENW010000000
VERSION     DBNENW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.326"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(384..>823)
                     /gene="perR"
                     /locus_tag="AC1LW8_002810"
     CDS             complement(384..>823)
                     /gene="perR"
                     /locus_tag="AC1LW8_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HIP4774548.1"
                     /translation="EIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHPTAD
                     EIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIICEQC
                     GKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT          263 a          120 c          109 g          331 t
ORIGIN      
        1 gagtcaacat aaaacaacac ttatttttgt ttaaaaatta ctgttgaagg gattattttc
       61 tattcgtaag tactatatgt atttaaagtg taattcctta cgctttttac ttcagtttta
      121 caaacaaact tatactcact ttatggatag ttatttagta gaccttcgtt tgaatatttt
      181 tatgtttatt tataaaggat actagttatt agttaatacc acttccgtag ttagaatgtt
      241 cttttcttct atttaaatat ttttcagaat aagcataaaa aagaagttaa acaactttca
      301 tgtcgtttaa cttcttaaat attacgctta cttatatgaa gtaagctaac gcctttttaa
      361 tttgtcatac taccaaagtt aaattattta tcttggcatt ctttacaaac tccataaatt
      421 tccattcgat gatgtgttac gtcaaagtca gtcatatgct gagctaatct ttcaatttca
      481 tttaactgtg gatattgaaa atcaacaatc ttaccacatt gttcacatat aatatgataa
      541 tgattatgtg tattaaagtc gaatcgactt gatgagtctc catatgttaa ttcttttaca
      601 attccaatat ctttaaacac tcttaagtta ttatatattg tcgcaacact tatatttgga
      661 aaatcaggtg aaagtgcttg ataaatttca tcagctgttg gatgagtatg tgaagaaatt
      721 aaataacgta atattgcttg tctttgaggt gtaattctta cgcctgcttg tcgcaatgat
      781 gcaattgatt cttctagttc atgttcaatt gattctattt caa
//
LOCUS       DBNENW010000323          815 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.327, whole genome shotgun sequence.
ACCESSION   DBNENW010000323 DBNENW010000000
VERSION     DBNENW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.327"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            34..507
                     /locus_tag="AC1LW8_002811"
     CDS             34..507
                     /locus_tag="AC1LW8_002811"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774549.1"
                     /translation="MSRILINAGDSLYYVSTTWIVLQWTQNPLLVGLTNTLLMIPMCI
                     SFLFGPLVDALPLKKTLFLTPLLQCLLLSLIASLNLIGYLNVYGLIILVAIAMFCTQI
                     GYPAQSKAIPLLVKKRSIIRCKFCYECRISRNRCYYEFNKWYYCCSNSFFSTIFY"
     gene            413..>815
                     /locus_tag="AC1LW8_002812"
     CDS             413..>815
                     /locus_tag="AC1LW8_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP4774550.1"
                     /translation="MSVAYQGTDVIMNSISGIIVAAIAFFPLYSINSLIFLIACLFML
                     NVNIPNITNPARNNPSYLQNLKDGVLGIKNSLLLVVALVSGVINFGLGLLYTWIPLKA
                     DHLGGAYYYGLLMALFSIGLIVGSLITPYIKK"
BASE COUNT          259 a          121 c          117 g          318 t
ORIGIN      
        1 ttatataaaa atcgttcgtt ttctaattta cttatgagta gaattttgat aaatgctggt
       61 gatagtctct attatgtttc tacaacttgg atagttttac aatggacgca aaacccttta
      121 ctagttggtt taactaatac tttattaatg attcctatgt gtatttcatt tttatttggt
      181 cctttagtag atgccttacc attaaagaag actctatttt taaccccttt attacaatgt
      241 ctattactat cactaattgc ttctttaaac ctaattggat acttaaatgt atacggcttg
      301 atcattttag tggcaattgc aatgttttgc actcaaatag gatacccagc tcagtctaaa
      361 gctatacctt tattagtaaa aaaaagatca attattagat gcaaattctg ctatgagtgt
      421 cgcatatcaa ggaacagatg ttattatgaa ttcaataagt ggtattattg ttgcagcaat
      481 agcttttttt ccactatatt ctattaattc actaatattt ctaatagctt gtttatttat
      541 gctgaatgta aatattccta atataactaa ccctgcaaga aataatcctt cttatttaca
      601 aaatttaaaa gacggtgtgc taggaattaa aaattcactc ctactagttg ttgcattggt
      661 aagtggtgtt ataaattttg gactaggact tctttataca tggatacctt taaaggctga
      721 ccatttaggt ggagcctatt attacggttt attaatggca ctattttcaa taggtttaat
      781 tgtaggttca ttaatcacac cttatataaa aaaaa
//
LOCUS       DBNENW010000324          806 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.328, whole genome shotgun sequence.
ACCESSION   DBNENW010000324 DBNENW010000000
VERSION     DBNENW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.328"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            9..>806
                     /locus_tag="AC1LW8_002813"
     CDS             9..>806
                     /locus_tag="AC1LW8_002813"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4774551.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSDILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNN"
BASE COUNT          324 a           80 c          137 g          265 t
ORIGIN      
        1 gtgattaaat gaagcaactg gttggaattc ccgaatcaat gttaattcct ttgatagctc
       61 gagcaaaaga gtacgaaaac gaaaaaccaa taataaaaga cgcactatct aaaaaaatat
      121 ttgatggttt agatgatatg tacaaaaatg ttacatgtga tgacatgtct caaattggaa
      181 ttagtatacg tactgtgata atagattgtg ttactaagag gcttatcaag gataataaag
      241 atttaatcgt ggtcaatata ggttgtggct tagatacaag gtttcaaaga tttaataaag
      301 aaaaaatatc atggatagat ttagatgtac cagaatcaat agaaatacga aaaacatttt
      361 ttaaagaaag taatagttat aagatgatat ctaaatctat gctagattac agttggattg
      421 atgatgtcaa aaattataaa ttttttaata gtaagtcaga tatattgttt attattgaag
      481 gtgtattgat gtattttgat gagagtgtaa tgactcaatt attggacact attatcaaaa
      541 agatgggaga tcataatttg acatttgcga ttgaattttg ctcaaaaaca attgcgaata
      601 atacaaagag acatcaatcg gtatcaaagt tatcctcaca acctgttttt aaatatggtt
      661 acaatgattt aaaaaaattg aatgaaattt taccaaatac aataagagta atacatgaat
      721 ataattattt tgactattat aagaatcgat ggggcttatt tggatattgt agatttatac
      781 cttatttaaa aaaaaggtta aacaat
//
LOCUS       DBNENW010000325          804 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.329, whole genome shotgun sequence.
ACCESSION   DBNENW010000325 DBNENW010000000
VERSION     DBNENW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.329"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..296
                     /locus_tag="AC1LW8_002814"
     CDS             <1..296
                     /locus_tag="AC1LW8_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HIP4774552.1"
                     /translation="YEPLIKKNNLNFKKGMKQARKGVGYKAAIEVHTTLFSRSSNFSK
                     DKKLDDVIDLSESTKKLHLNFENTKIFLQLAKSTISTNRVNYSDNESIRIEVE"
     gene            354..>804
                     /locus_tag="AC1LW8_002815"
     CDS             354..>804
                     /locus_tag="AC1LW8_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HIP4774553.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGIEVYVHCDDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKE"
BASE COUNT          320 a          111 c          157 g          216 t
ORIGIN      
        1 attacgaacc cctaataaag aaaaataatc tgaattttaa aaaaggtatg aaacaagcaa
       61 ggaaaggagt aggctataaa gctgcaatag aagtacatac aacattgttt tcgagaagta
      121 gtaacttttc aaaggacaaa aaattagatg atgttataga tttgtctgaa agtacgaaaa
      181 agttacacct taattttgaa aatacgaaaa tatttttaca actagcaaaa tctactatta
      241 gtactaatcg agttaattac agtgataatg agtctataag gattgaggtc gaatgacttg
      301 aaatcagcta atttctctat attctaaaca aacacataaa tgggtgatga gctatgttta
      361 aaagaactaa actaatctta atagcaacat tactgctatc aggatgttca actaccaata
      421 acgaatccaa caatgaaaca aaatcagtac cagaagaaat ggaagcttca aaatatgttg
      481 gacaaggctt ccaaccacct gcagaaaaag atgcgattga atttgcgaag aagcatcgta
      541 aagaatttga aaaagtaggt gaacaattct ttaaagacaa ctttggactg aaggtaaagg
      601 ctaccaatgt tgtaggtaaa gatgatggta tagaagttta tgtgcattgc gatgatcatg
      661 gcattgtatt taatgcaagt ttgcccttgt acaaagatgc catccatcaa aaaggatcaa
      721 tgcgtagtaa tgacaacggt gatgatatga gtatgatggt gggtacagtg ctgagtggct
      781 ttgaatatcg agcgcaaaaa gaaa
//
LOCUS       DBNENW010000326          801 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.330, whole genome shotgun sequence.
ACCESSION   DBNENW010000326 DBNENW010000000
VERSION     DBNENW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.330"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>801
                     /locus_tag="AC1LW8_002816"
     CDS             <1..>801
                     /locus_tag="AC1LW8_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MrcB family domain-containing protein"
                     /protein_id="HIP4774554.1"
                     /translation="IKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLF
                     HPEGEGIYLSLNQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAE
                     NKDSSYDLKNDYPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKT
                     SYDSLVNSIDEIQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIE
                     KSNKENKLTGKVGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQH"
BASE COUNT          350 a           93 c          140 g          218 t
ORIGIN      
        1 ataaaagaag ataaatattc agttgtaggg aaggttgcta ctgggaacta tagtaaagtt
       61 ccttggattt caatatatga tgagaatata acaaaagaaa caaaggatgg atattatttg
      121 gtatatcttt ttcatccgga aggagaaggc atatacttat ctttgaatca aggatggtca
      181 aagataagtg atatgtttcc gcgggataaa aatgctgcaa aacaaagagc attaacttta
      241 tcttccgaac tcaataaata tattacatca aatgaattta atactggaag attttattac
      301 gcagaaaata aagattcatc ttatgattta aaaaatgatt atccatcagg atattctcat
      361 ggatcaataa gattcaaata ttatgatttg aatgaaggat tcacagaaga agatatgcta
      421 gaggatttaa agaaattttt agaactattt aatgaattag cttcaaaagt tacaaaaaca
      481 tcctatgata gcttggtcaa tagcatagac gaaatacagg aagacagcga aattgaagaa
      541 attagaacag cacaaaaaga taagacactc aaggaagtgg aagcacctaa aggaataatt
      601 ccaaaatata aaaaaggtgt atcaaagact actaaaaatg attcagaaat tgaaaaatca
      661 aataaagaga ataaattaac cggtaaagtt ggagaaaaat tagcgctaaa ttactttaat
      721 gagctaattg ataataaaat agacgaagat aagaaagaac agtttaggaa tattttaaat
      781 gataatccag gctctcaaca c
//
LOCUS       DBNENW010000327          800 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.331, whole genome shotgun sequence.
ACCESSION   DBNENW010000327 DBNENW010000000
VERSION     DBNENW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.331"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(130..758)
                     /locus_tag="AC1LW8_002817"
                     /pseudo
     CDS             complement(130..758)
                     /locus_tag="AC1LW8_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
BASE COUNT          275 a          128 c           95 g          302 t
ORIGIN      
        1 tattttataa aaatagacca cgcataccta tctttaaacg gacaatgttt ataaatgagt
       61 ttgcatggtc ttgaattgta ttagttttct tttggtttta ataaatcgac tagattttca
      121 caatatttat caaatatgta ttcctaaatt atacagcctt aatccagcag ctactttcga
      181 aacttccaac ttagttgata taaggttcaa tagtttgttt cattcttttt cagataaacc
      241 agaacttaaa ttgatattat tgacttcata aaaattatag actaatgcct ctatttgctt
      301 tttaggcata agtaagtcga ctgaaaactg atttacgtcg ctttcataaa tcatttcatg
      361 taaattcttt agactattat cgttgttatc tttaattttg tataaataac ggccgagttc
      421 acgagctatt gcaaatcttg tattattaat cgagtgatta ttattgatat aaatcgttct
      481 tccacttaaa taacccgaag tattaccctc cattttaata tatttaactt ttaaattaag
      541 ttgaaataat agcttgtcta tgtcaatagc aaagtgttca gaagtaataa aaagttgatc
      601 cattttgtcc ttaactaatg gctgaattaa ttgaactatt tctggttcta aaatatcttc
      661 ataatgaact ttctcaataa ctttcaattg attcccctcc ctaactaaaa aatcgaacct
      721 acatcattta ataaatttaa aactataggt gtaaacatta ttaaaataaa acccctccac
      781 tactaaatgt aacggaggga
//
LOCUS       DBNENW010000328          794 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.332, whole genome shotgun sequence.
ACCESSION   DBNENW010000328 DBNENW010000000
VERSION     DBNENW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.332"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..660
                     /gene="seh"
                     /locus_tag="AC1LW8_002818"
     CDS             <1..660
                     /gene="seh"
                     /locus_tag="AC1LW8_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type H"
                     /protein_id="HIP4774555.1"
                     /translation="KAEDLHDKSELTDLALANAYGQYNHPFIKENIKSDEISGEKDLI
                     FRNQGDSGNDLRVKFATADLAQKFKNKNVDIYGASFYYKCEKISENISECLYGGTTLN
                     SEKLAQERVIGANVWVDGIQKETELIRTNKKNVTLQELDIKIRKILSDKYKIYYKDSE
                     ISKGLIEFDMKTPRDYSFDIYDLKGENDYEIDKIYEDNKTLKSDDISHIDVNLYTKKK
                     V"
BASE COUNT          339 a           79 c          137 g          239 t
ORIGIN      
        1 aaagcagaag atttacacga taaaagtgag ttaacagatt tagctttagc taatgcatat
       61 ggtcaatata atcacccatt cattaaagaa aatattaaga gtgatgaaat aagtggagaa
      121 aaagatttaa tatttagaaa tcaaggtgat agtggcaatg atttgagagt aaagtttgca
      181 actgctgatt tagctcagaa gtttaaaaat aaaaatgtag atatatatgg ggcatctttt
      241 tattataagt gtgaaaaaat aagtgaaaat atttctgaat gtctatatgg aggtacaaca
      301 ctaaatagtg aaaaattggc acaggaaagg gtgattggtg ctaatgtttg ggtagatggt
      361 attcaaaaag aaacagaatt aatacgaaca aataagaaaa atgtgacatt gcaagaatta
      421 gatataaaga tcagaaaaat attgtccgat aaatataaaa tttattataa agacagcgaa
      481 ataagtaaag gtctaattga atttgatatg aaaactccta gagattactc attcgacatt
      541 tatgatttaa aaggagaaaa tgactatgag atagataaaa tttatgaaga caataaaact
      601 ttaaaatctg atgatataag tcatattgat gtaaatctat atactaagaa aaaagtataa
      661 tcaatgaaaa tttctagatc aatgttagtg ttaattgtca tgtctttttt ccgttatgga
      721 attcatttaa gcatttttct caaacaagta catatactcc aaaagcagaa actctaaaga
      781 acaagtccga ctta
//
LOCUS       DBNENW010000329          786 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.333, whole genome shotgun sequence.
ACCESSION   DBNENW010000329 DBNENW010000000
VERSION     DBNENW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.333"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            4..690
                     /locus_tag="AC1LW8_002819"
     CDS             4..690
                     /locus_tag="AC1LW8_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIP4774556.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVKRSGSEHPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          295 a          109 c          157 g          225 t
ORIGIN      
        1 gttatgacag aatacttatt aagtgctggc atatgtatgg caattgtttc aatattactt
       61 atagggatgg ctatcagtaa tgtttcgaaa gggcaatacg caaagaggtt tttctttttc
      121 gctactagtt gcttagtgtt aactttagtt gtagtttcaa gtctaagtag ctcagcaaat
      181 gcatcacaaa cagataacgg cgtaaagaga agtggttctg aacatccaac agtatatagt
      241 gcaacttcaa ctaaaaaatt acataaagaa cctgcgacat taattaaagc gattgatggt
      301 gatacggtta aattaatgta caaaggtcaa ccaatgacat ttagactatt attggtggat
      361 acacctgaaa caaagcatcc taaaaaaggt gtagagaaat atggtcctga agcaagtgca
      421 tttacgaaaa aaatggtaga aaatgcaaag aaaattgaag tcgagtttga caaaggccaa
      481 agaactgata aatatggacg tggcttagcg tatatttatg ctgatggaaa aatggtaaac
      541 gaagctttag ttcgtcaagg cttggctaaa gttgcttatg tttataaacc taacaataca
      601 catgaacaac ttttaagaaa aagtgaagca caagcgaaaa aagagaaatt aaatatttgg
      661 agcgaagaca acgctgattc aggtcaataa tgctcattgt aaaagtgtca ctgctgctag
      721 tggcactttt ataatttttg gatcacgata tgatttatta tcaattcaga attaaaaaag
      781 taaata
//
LOCUS       DBNENW010000330          777 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.334, whole genome shotgun sequence.
ACCESSION   DBNENW010000330 DBNENW010000000
VERSION     DBNENW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.334"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>777
                     /locus_tag="AC1LW8_002820"
     rRNA            <1..>777
                     /locus_tag="AC1LW8_002820"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          223 a          153 c          224 g          177 t
ORIGIN      
        1 ttgtctctct tgagtggatc ctgagtacga cggagcacgt gaaattccgt cggaatctgg
       61 gaggaccatc tcctaaggct aaatactctc tagtgaccga tagtgaacca gtaccgtgag
      121 ggaaaggtga aaagcacccc ggaaggggag tgaaatagaa cctgaaaccg tgtgcttaca
      181 agtagtcaga gcccgttaat gggtgatggc gtgccttttg tagaatgaac cggcgagtta
      241 cgatttgatg caaggttaag cagtaaatgt ggagccgtag cgaaagcgag tctgaatagg
      301 gcgtttagta tttggtcgta gacccgaaac caggtgatct acccttggtc aggttgaagt
      361 tcaggtaaca ctgaatggag gaccgaaccg acttacgttg aaaagtgagc ggatgaactg
      421 agggtagcgg agaaattcca atcgaacctg gagatagctg gttctctccg aaatagcttt
      481 agggctagcc tcaagtgatg attattggag gtagagcact gtttggacga ggggcccctc
      541 tcgggttacc gaattcagac aaactccgaa tgccaattaa tttaacttgg gagtcagaac
      601 atgggtgata aggtccgtgt tcgaaaggga aacagcccag accaccagct aaggtcccaa
      661 aatatatgtt aagtggaaaa ggatgtggcg ttgcccagac aactaggatg ttggcttaga
      721 agcagccatc atttaaagag tgcgtaatag ctcactagtc gagtgacact gcgccga
//
LOCUS       DBNENW010000331          748 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.335, whole genome shotgun sequence.
ACCESSION   DBNENW010000331 DBNENW010000000
VERSION     DBNENW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.335"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            61..>748
                     /locus_tag="AC1LW8_002821"
     CDS             61..>748
                     /locus_tag="AC1LW8_002821"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIP4774557.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDI"
BASE COUNT          285 a           99 c          156 g          208 t
ORIGIN      
        1 taagaaaaag acatgcagat gttgttaaaa ttttaataag aatcaaggag gcaatattat
       61 atggctaaac taaatgtaga agtatttgcg gacggtgcag atattgaaga aatgaaagca
      121 gcttataaaa acaaacaagt ggatggtttt acaacaaatc ctagtttaat ggcgaaagcg
      181 ggcgtaacag attacaaagc ttttgctgaa gaagctgtga aagaaattcc agatgcttca
      241 atttcatttg aagtatttgc agacgattta gaaactatgg aaaaagaagc agcaattcta
      301 aaacaatatg gcgaaaatgt atttgttaaa attcctattg taaatacaaa aggtgaatca
      361 acgattcctt taattaaaaa gctttcagct gacaatgtga gattaaacgt tacggctgtt
      421 tacacaattg aacaagttaa agaaataact gaagcagtaa ctgaaggtgt gccaacatat
      481 gtttcagtat ttgcaggacg tattgcagat acaggcgtag atccattacc attaatgaaa
      541 gaggctgtaa aagttacgca tagtaaagac ggcgttaaat tattatgggc aagttgccgc
      601 gaattattca atgtgattca agctgatgaa attggtgcag atattattac atgccctgca
      661 gatgttgtga aaaaagtgaa tacaaactta ggtcgcgata ttaacgaatt atcagtagat
      721 acagttaaag gctttgcgaa agatattc
//
LOCUS       DBNENW010000332          738 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.336, whole genome shotgun sequence.
ACCESSION   DBNENW010000332 DBNENW010000000
VERSION     DBNENW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.336"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>738
                     /locus_tag="AC1LW8_002822"
     CDS             <1..>738
                     /locus_tag="AC1LW8_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MrcB family domain-containing protein"
                     /protein_id="HIP4774558.1"
                     /translation="LPKQLKAHIKEDKYSVVGKVATGNYSKVPWISIYDENITKESKD
                     GYYLVYLFHPEGEGIYLSLNQGWSKISNMFPRDKNAAKQRALTLSSELNKYITSNEFN
                     TGRFYYAENKDSSYDLKNDYPSGYSHGSIRIKYYDLNEGFTEEEMLYDLKKFLELFNE
                     LASKVTKTFYDGLVNRIDEIQEDSEIEEIRIAQIDKTLKQVEAPKGVIPKYKKGVSKT
                     TKNDSEIEKSNKENKLTGKVGEKLALK"
BASE COUNT          322 a           85 c          128 g          203 t
ORIGIN      
        1 agttacccaa gcagttaaaa gcgcatataa aagaagataa atattcagtt gtagggaagg
       61 ttgctactgg gaactatagt aaagttcctt ggatttcaat atatgatgag aatataacaa
      121 aagaatcaaa ggatggatat tatttggtat atctttttca tccagaagga gaaggcatat
      181 acttatcttt gaatcaagga tggtcaaaga taagtaatat gtttccgcgg gataaaaatg
      241 ctgcaaaaca aagagcatta actttatctt ccgaactcaa taaatatatt acatcaaatg
      301 aatttaatac tggaagattt tattacgcag aaaataaaga ttcatcttat gatttaaaaa
      361 atgattaccc atcaggatat tctcatggat caataagaat caaatattat gatttgaatg
      421 aagggttcac agaagaagaa atgctatatg atttaaagaa atttttagaa ctatttaatg
      481 aattagcttc aaaagttaca aaaacattct atgatggctt agtcaataga atagacgaaa
      541 tacaggaaga tagtgaaatt gaagaaatta gaatagcaca aatagataag acactcaagc
      601 aagtggaagc acctaaagga gtaattccaa aatataaaaa aggtgtatca aagactacta
      661 aaaatgattc agaaattgaa aaatcaaata aagagaataa attaaccggt aaagttggag
      721 aaaaattagc gctaaaat
//
LOCUS       DBNENW010000333          727 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.337, whole genome shotgun sequence.
ACCESSION   DBNENW010000333 DBNENW010000000
VERSION     DBNENW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.337"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..690)
                     /gene="sarU"
                     /locus_tag="AC1LW8_002823"
     CDS             complement(<1..690)
                     /gene="sarU"
                     /locus_tag="AC1LW8_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HIP4774559.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVL
                     IKINKIQYNTIK"
BASE COUNT          239 a          102 c           81 g          305 t
ORIGIN      
        1 tttaatagtg ttatattgta ttttatttat tttgattaac acaatccttt catctgcaag
       61 ggatcgttct ttgattagca tacctttatt atttaatcta ttaactgacc taacaatttg
      121 aggatacata aatctaatac tttcaaacaa atcttttaaa tttagtatct cattttttcg
      181 agatgatatt acatataaga tagataaatc aagaaatgtt aaattaaatc gatattttaa
      241 aaaatctttt gagtacataa tgttcataaa aagtataaaa aattctttgc tatattttgg
      301 agcccattga tattttatgt agtctctttc cttattaaat cctttaatta ttttatctaa
      361 ctcattaata acacaagcaa tctttttacg ttgtattgga gtaactgaaa caaaaatacg
      421 gcgttcatct gcttcgtttc tagacttatt tataaatcct tttttagata gtgacttaat
      481 gttctttaca acatcagatt gtttgtaaag tatgtcacca atgatttctt ttagtgaaat
      541 actgtgttca tttttgtaat aaaaatatgc taatatcaac aattctttta atgataattt
      601 atattgttgc tttaactctt gagtgagaaa aaatatgtac gcttctacat ttatgaattt
      661 attgaccttt tcgaaagttt gatagtccac cttacattct cctactattt tctgatgtcc
      721 caatact
//
LOCUS       DBNENW010000334          718 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.338, whole genome shotgun sequence.
ACCESSION   DBNENW010000334 DBNENW010000000
VERSION     DBNENW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.338"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            186..599
                     /locus_tag="AC1LW8_002824"
     CDS             186..599
                     /locus_tag="AC1LW8_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIP4774560.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDCIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          290 a           87 c          118 g          223 t
ORIGIN      
        1 taagattaca ctgtagattg agaaatttaa agtaaataaa aaatacatta tacaataaat
       61 ataaaactga aaaataaatt ttatggcaat ttcgtgtcgc gaggacttat caagtgacaa
      121 ccttgtttaa atgtttttaa attaggaaaa atgactataa gtactattaa aggagcgaat
      181 aaattatgcc aatcgtatat tatgatggaa actgtatata ttgttataac tatgtcattt
      241 ggttaattca acatgaccta ccaagaaatt atcaatttgc cacgttaaaa ggtgaagttg
      301 gccaacaatt ttttaaacaa catccagaag cggcaaataa aaatagtgtg attttacaga
      361 aaggtgattg tatatatttt gaatcgcagg cgattattaa actaatcaca gcactaccta
      421 atcgaactaa attattgggc gtaggtttat ggcttgtacc taaaccagtc cgtaatttcg
      481 gttatcgaat gtttgctaat aatagaaata gaatgtggaa aacaacatgg caccaaccaa
      541 atgattatga aaaatcgttc tttttagatg ataatgcgaa agtaaaactt actgattgat
      601 aaaacatact tgctaattga taatggatat attagatgat gaattaaaat ttaggcattt
      661 aaatagcgga acactttacg gttagattag aataattata aaaaagagag taaaaaca
//
LOCUS       DBNENW010000335          717 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.339, whole genome shotgun sequence.
ACCESSION   DBNENW010000335 DBNENW010000000
VERSION     DBNENW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.339"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>717
                     /locus_tag="AC1LW8_002825"
     CDS             <1..>717
                     /locus_tag="AC1LW8_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HIP4774561.1"
                     /translation="MGNLIVTNKYTPETTSISGEKVWDDKDNQDGKRPEKVSVNLLAN
                     GEKVKAVDVTSETNWKYEFKDLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTP
                     GETSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTAKLNESNNWTHTWTGLDEK
                     AKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSINGEKVWDDKDNQDG
                     KRPEKVSINLLANGEKVKTL"
BASE COUNT          329 a          102 c          154 g          132 t
ORIGIN      
        1 tatgggtaac ttgattgtga cgaataaata tacgccagaa acaacatcaa ttagcggtga
       61 aaaagtatgg gacgacaaag acaatcaaga tggtaagaga ccagaaaaag tcagtgtgaa
      121 tttattagct aacggagaga aagtaaaagc ggtagatgtg acatctgaaa caaactggaa
      181 gtacgaattt aaagacttac cgaagtatga tgaaggaaag aaaatagaat atacagtgac
      241 cgaagatcac gtaaaagact acacaacaga catcaacggt acaacaataa cgaacaagta
      301 tacaccagga gagacatcgg caacagtaac aaaaaattgg gatgacaata ataaccaaga
      361 cggaaaacga ccaactgaaa ttaaagttga gttatatcaa gatggaaaag caacaggaaa
      421 aacggcaaaa ttaaatgaat ctaataactg gacccatacg tggacaggat tagatgaaaa
      481 agcaaaaggt caacaagtaa aatacacagt cgaggaatta acaaaggtca aaggttatac
      541 aacacatgtg gataacaatg atatgggcaa cttgattgtg acgaataaat atacgccaga
      601 aacaacatca attaacggtg aaaaagtatg ggacgacaaa gacaatcaag atggtaagag
      661 accagaaaaa gtcagtataa atttattggc taatggagag aaagtaaaaa cgttaga
//
LOCUS       DBNENW010000336          715 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.340, whole genome shotgun sequence.
ACCESSION   DBNENW010000336 DBNENW010000000
VERSION     DBNENW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.340"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..>715)
                     /locus_tag="AC1LW8_002826"
     CDS             complement(<1..>715)
                     /locus_tag="AC1LW8_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP4774562.1"
                     /translation="LNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGL
                     NFKEIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQE
                     LRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDY
                     LFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKE
                     LRAFVTGNAPAKLTHANLCN"
BASE COUNT          207 a          126 c           93 g          289 t
ORIGIN      
        1 tattgcataa gttcgcatga gttaatttag ctggtgcatt acctgtgaca aatgctcgta
       61 gttctttaaa atttaaataa taattcataa aaaacaaatt atgatcatta cttttaacta
      121 catgcgcatg attatttacc cagtattgcc cattagcaat aaagctactc gtctcaaact
      181 gcccccattt tgcaccatct tctcctatta gtaatcgttc ttcattattg aataaataat
      241 cttttacgta atcaataatt ccagttgcac catagtaagg gtataacccc ttttctctta
      301 atgaagaagt aattggtttt cttctattat tttcaaagat aaagatgtct tttatgaatt
      361 tgttttccca ctctggatat tcttcaccat tctcatcttt gaatcgcaat tcttgtgtga
      421 agattttctg catatagcct tttttctgtt gttgaagcaa ttcaagtttt tgttcttcta
      481 attcaatttg tcggtcgagt ttgctgaaga acttgcctat tttttgctgt tcttcaaata
      541 tagttggggt gaagattttt aaattagcaa tttctttgaa gtttagacct tctcgactac
      601 ctccactttg tgcaaggaaa attttccttt taccttttct tgataataga tactgtccaa
      661 aaaaattata ataatactct tttttcaatc taataataca tacatgttga tttaa
//
LOCUS       DBNENW010000337          694 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.341, whole genome shotgun sequence.
ACCESSION   DBNENW010000337 DBNENW010000000
VERSION     DBNENW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.341"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>694
                     /locus_tag="AC1LW8_002827"
     CDS             <1..>694
                     /locus_tag="AC1LW8_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP4774563.1"
                     /translation="KEEIDFSKIDNNELFLISGKTGSGKTMIFDAMTYALFGKASTEQ
                     REENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGNTTKTNAKFDVFEMVD
                     GKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFLISNSREKQGILRTLF
                     DSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDDNIKGLLEVATQQIDK
                     LIENIPLLQARSK"
BASE COUNT          282 a           83 c          130 g          199 t
ORIGIN      
        1 aaagaagaaa ttgatttttc taaaattgat aataatgaat tgtttttaat aagtggtaag
       61 actggatcgg gtaaaacaat gatttttgat gcaatgactt atgccttgtt tggtaaagca
      121 tcaactgaac aaagagaaga aaatgatttg agaagtcatt tcgctgatgg taaacagccg
      181 atgtcagtaa catttgaatt tcaattaaat catcgaattt ataaagtgca tagacaaggc
      241 ccttatatca aagaaggtaa tacaacaaaa acgaacgcta aatttgatgt atttgagatg
      301 gtggatggca agtatgaaat tagagaaagt aaagtaattt caggtaccca attcattatt
      361 gaattattag gagtaaatgc agatcaattc cgacaattgt ttattttgcc tcaaggtgaa
      421 ttcaaacgct ttttaatatc aaacagtcgt gaaaagcaag ggatattaag aacactgttt
      481 gacagtgaaa aatttgaagc tatacgagaa atattaaaag aagaagtaaa aaaagaaaaa
      541 gctcaaatcg agaatagata tcaacaaatt gaccttttat ggcaagaaat tgaatcattt
      601 gatgatgaca atataaaagg cttattagag gttgccactc aacagataga caaattgatt
      661 gaaaatatac cacttttaca agctaggtcg aaag
//
LOCUS       DBNENW010000338          689 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.342, whole genome shotgun sequence.
ACCESSION   DBNENW010000338 DBNENW010000000
VERSION     DBNENW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.342"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            36..>689
                     /locus_tag="AC1LW8_002828"
     CDS             36..>689
                     /locus_tag="AC1LW8_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP4774564.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPVFNFMTSFVESTNDKVKTHLANGFK
                     "
BASE COUNT          236 a          100 c           95 g          258 t
ORIGIN      
        1 ttattgtatt tatatgaaga aaaggaggca ccactttgtt tacttacttt aaatcagcat
       61 ttaaaaatgc taaaccacaa ttattaataa cattgatata tgcacttata gcatttgctg
      121 taattgcagt tgtatattta cttgctaatt tccagcttgc taagtatgta caaactattg
      181 caatttattc acaatttggg caaaaaccac cagtagatgc atatttaaaa gttatcgctg
      241 tattacttat agcagctgta gtttcactgt ttgttttagt tcaaattttt atcggaatta
      301 caaatgttat gaaacgagca atgagtcatg aaaaagtaaa atttaccgat ttatttattg
      361 catttaaaaa aggtaattat ttaaagagcg ttctgatagg tcttgtatct atagcaatga
      421 ttatcgttct ttcacttctg acatcattac tatacaaatt attttctcca gtatcagaaa
      481 tgattatgaa ttcagtacaa tcatcatatg cagatagtac acacctgatt ggtattgcta
      541 ttacaactca aagcatcata atcattgttg ttttattgat taaagcaatt attacttggt
      601 tattgttaat acctgttttc aactttatga ctagtttcgt tgaatcaact aacgataaag
      661 taaaaacaca cttagctaat ggatttaaa
//
LOCUS       DBNENW010000339          679 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.343, whole genome shotgun sequence.
ACCESSION   DBNENW010000339 DBNENW010000000
VERSION     DBNENW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.343"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..647
                     /locus_tag="AC1LW8_002829"
     CDS             <1..647
                     /locus_tag="AC1LW8_002829"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP4774565.1"
                     /translation="KFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITHY
                     MGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQG
                     KKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNSQ
                     QTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
BASE COUNT          250 a          102 c          130 g          197 t
ORIGIN      
        1 tgaagtttga caattatatt tttgattttg atggtacgtt ggcagacacg aaaaaatgtg
       61 gtgaagtagc aacacaaagt gcatttaaag catgtggctt aacggaacca tcatctaaag
      121 aaataacgca ttatatggga atacctattg aagaatcatt tttaaaatta gcagaccgac
      181 cattagatga agcagcatta gcaaagttaa tcgatacatt tagacataca tatcaatcta
      241 ttgaaaagga ctatatttat gaatttgcgg gtataactga agccattaca agtttgtata
      301 accaagggaa aaaacttttc gtggtgtcta gtaagaagag tgatgtatta gaaagaaatt
      361 tatcggctat tggattaaat cacttgatta ccgaagctgt tggatccgat caagtaagtg
      421 catataaacc aaatcctgaa ggcatacaca caattgtgca acgctacaat ttaaatagcc
      481 aacaaacggt ttatattggt gattcaacat ttgatgttga gatggcgcaa catgctggtg
      541 taccatctgc agcagtaaca tggggagcac atgatgcaag gtcattactt cattcaaatc
      601 cggattttat aatcaatgat ccatcagaaa ttaatacagt attataaaat ttgttaaaac
      661 agagaatacc atggttaaa
//
LOCUS       DBNENW010000340          674 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.344, whole genome shotgun sequence.
ACCESSION   DBNENW010000340 DBNENW010000000
VERSION     DBNENW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.344"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            249..623
                     /locus_tag="AC1LW8_002830"
     CDS             249..623
                     /locus_tag="AC1LW8_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HIP4774566.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
BASE COUNT          294 a           66 c          110 g          204 t
ORIGIN      
        1 atcaaatagt tattgcataa tttattatga tgacataatt ttcagtagat agttgtaaat
       61 taacactgaa atagatgttt atattaagaa gactttaata tcataattaa tcaaaatctt
      121 gcgtcaaaca tttaaacgaa ttttaagttg ataacaattg gcaaaacatt gttcaaacat
      181 cacaatgata aagcatatta tcagtattgt agtgtgtgga aaatgacagc catctaagga
      241 gaaaaatgat gaaaagaata ttggtagtat ttttaatgtt agcaattata ttggcaggtt
      301 gttctaataa aggtgaaaag tatcaaaaag atattgataa agtgtacaaa gaacagaatc
      361 aaatgaataa aattgcctcg aaagtacaaa acactattaa aacagacatt aaacaagaag
      421 acagtaatac acatgtttat aaagatggta aagtcattgt tattggtatt caattatata
      481 aagatcgtga aaaaatgtat tatttcgcat atgaaataaa agatggtaag gcagagatta
      541 atagagaaat agacccaatt aagtatatga aagaccataa agcagattat gaagatgaaa
      601 atgtagaagt ggaaaaagat taacataggc tttctttaaa acttttaacc tgtttcaaaa
      661 agtaaatgag cttt
//
LOCUS       DBNENW010000341          670 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.345, whole genome shotgun sequence.
ACCESSION   DBNENW010000341 DBNENW010000000
VERSION     DBNENW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.345"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..546
                     /locus_tag="AC1LW8_002831"
     CDS             <1..546
                     /locus_tag="AC1LW8_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP4774567.1"
                     /translation="SFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTMLQ
                     FAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSAL
                     ANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAII
                     LLYALGSILAVYCISHFKRAE"
     gene            550..>670
                     /locus_tag="AC1LW8_002832"
     CDS             550..>670
                     /locus_tag="AC1LW8_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP4774568.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIIS"
BASE COUNT          212 a           82 c          112 g          264 t
ORIGIN      
        1 agttttagtt tactaacttt tccattagat attattaatg aaaaacaaaa tgaatggcgc
       61 caaagattaa tggtaacacc atttactttt actagttatt atatttcaaa agtagtgaaa
      121 actatgctgc aatttgcaat agcgatatta gttattttta tggttggaca tttttataaa
      181 ggtgttgcaa tgagtgcagt tcaatggtta gagtcaggaa tatttttatg gttaggtgcg
      241 tctctattaa taacttttgg catattattt tctttgttaa atgatattca aaaaacaagt
      301 gctttagcta atatcgtaac aattggttta gcagtattag gtggattgtg gtttccgata
      361 aacacatttc caaattggct tcaacatgtt gctcatgttt taccgagcta tcatttgcgt
      421 aaactaggtg tagatattgc ttcaaatcat catatcaatt taatatcatt tgctataata
      481 ctcttgtatg ctttagggag tatattagca gtatattgta ttagtcattt taaaagggcg
      541 gaataaaata tgaaattttt aaaagatact tcaattgctg aaatatcgtc tatactttat
      601 ctgatttttc ctattgccgg tatatttttt aatgaagtat atggtcccaa atggttgtat
      661 attatatcag
//
LOCUS       DBNENW010000342          656 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.346, whole genome shotgun sequence.
ACCESSION   DBNENW010000342 DBNENW010000000
VERSION     DBNENW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.346"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            24..521
                     /gene="efb"
                     /locus_tag="AC1LW8_002833"
     CDS             24..521
                     /gene="efb"
                     /locus_tag="AC1LW8_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HIP4774569.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
BASE COUNT          278 a           89 c          101 g          188 t
ORIGIN      
        1 taataatttg gaggataatt aacatgaaaa ataaattgat agcaaaatct ttattaacaa
       61 tagcggcaat aggtattact acaactacaa ttgcgtcaac agcagatgcg agcgaaggat
      121 acggtccaag agaaaagaaa ccagtgagta ttaatcacaa tatcgtagag tacaatgatg
      181 gtacttttaa atatcaatct agaccaaaat ttaactcaac acctaaatat attaaattca
      241 aacatgacta taatatttta gaatttaacg atggtacatt cgaatatggt gcacgtccac
      301 aatttaataa accagcagca aaaactgatg caactattaa aaaagaacaa aaattgattc
      361 aagctcaaaa tcttgtgaga gaatttgaaa aaacacatac tgtcagtgca cacagaaaag
      421 cacaaaaggc agtcaactta gtttcgtttg aatacaaagt taagaaaatg gtcttacaag
      481 agcgaattga taatgtatta aaacaaggat tagttaaata aaacttcaat cgttgctgtt
      541 atctggaaat aattaattaa atgttatgtt aatttttgtt aatgaaaaaa gtaatctatt
      601 taatgacagg ttaatgtaat tgtcctgaaa ttgactatat actcagtaag tatcaa
//
LOCUS       DBNENW010000343          647 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.347, whole genome shotgun sequence.
ACCESSION   DBNENW010000343 DBNENW010000000
VERSION     DBNENW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.347"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..160
                     /locus_tag="AC1LW8_002834"
     CDS             <1..160
                     /locus_tag="AC1LW8_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HIP4774570.1"
                     /translation="TKKSPVLKLHIDGDIKGSSIGYKNIEFNFSKVKDEETAVRDFVN
                     FGPSDGVS"
     gene            208..>647
                     /locus_tag="AC1LW8_002835"
     CDS             208..>647
                     /locus_tag="AC1LW8_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774571.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKSILLDKVEDP"
BASE COUNT          276 a           61 c          131 g          179 t
ORIGIN      
        1 aacaaaaaaa tctccagtat taaaattaca tatagatggt gacataaaag gaagttcaat
       61 tggatataaa aatatagaat ttaatttttc aaaagtaaaa gacgaagaaa cagctgttag
      121 agattttgtg aattttggac catctgatgg agttagctaa agataattag atgtagaaat
      181 aattctaaaa cagagagacg tgaaacgatg ggatatttaa aaaggattgg aatgtgtata
      241 agcctgttga ttgtaatcat ttttgtaaca tcttgcggtg gtggtaataa aatcactgga
      301 gattcaaaag aaacacaaat caaaaagagt tttgcgaaaa cgttagatat gtacccaatc
      361 aaaaatcttg aagatttata cgacaaagaa ggatatcgag atggcgaatt taaaaaaggt
      421 gacaaaggga tgtggacgat atacacagat ttcgctaaaa gtaataaacc gggtgaattg
      481 gatgatgaag gtatggtttt aaatctggat agaaatactc gaacggctaa gggatattat
      541 tttgttaaga aattttatga aaaggataaa ttacctgata gaaaaaatta taaagttgaa
      601 atgaaaaata ataaaagtat cttattagac aaggtagaag atccaaa
//
LOCUS       DBNENW010000344          642 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.348, whole genome shotgun sequence.
ACCESSION   DBNENW010000344 DBNENW010000000
VERSION     DBNENW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.348"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..111
                     /locus_tag="AC1LW8_002836"
     CDS             <1..111
                     /locus_tag="AC1LW8_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774572.1"
                     /translation="FSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            123..359
                     /locus_tag="AC1LW8_002837"
                     /pseudo
     CDS             123..359
                     /locus_tag="AC1LW8_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669339.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAR2788 family putative toxin"
     gene            373..>642
                     /locus_tag="AC1LW8_002838"
     CDS             373..>642
                     /locus_tag="AC1LW8_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HIP4774573.1"
                     /translation="MCFDINNLDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYSEPYKKTNVIKKFTS"
BASE COUNT          256 a           69 c          102 g          215 t
ORIGIN      
        1 ttttctatta ttacattaat tacaggtatc attatgcata ttacaatgta ctttgtacca
       61 tttgaatctc ggaaaatgcc actatttatg acgataattt ttacaatcta aaagcagtaa
      121 agactcataa tataaccaaa aacattaata aaagtttgtt aaatgataag gggatgagta
      181 ttggtaaatt tactcaaaag gtttaaggga aaaatcctac ctggagagat ccaaaaacta
      241 aatggactat ttccaaaaat aaagggcaat cacatggtgg ttcttattgg aagttaataa
      301 ataacaaagg aaaaagaata gcttctttaa ctaaagaagg aaaaatctta agggaataag
      361 gtgttgtaaa ttatgtgctt tgatataaat aatttggata taaaaaagtt gaattttaga
      421 aaggtaaaaa acgcaattca tttaagttta agtggcgata aataccagtt tttggaggac
      481 ggtaaaacta tcaataatac ttatttttta gctgtatatg ataatgcaat aaacattttt
      541 gaagatttgt tttcgccttc tgattctata aatttggttc atgtagtata tgtttatagt
      601 gaaccttata agaaaacaaa tgttataaaa aaatttacat cg
//
LOCUS       DBNENW010000345          641 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.349, whole genome shotgun sequence.
ACCESSION   DBNENW010000345 DBNENW010000000
VERSION     DBNENW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.349"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            267..>641
                     /locus_tag="AC1LW8_002839"
     CDS             267..>641
                     /locus_tag="AC1LW8_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774574.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGF"
BASE COUNT          224 a           75 c          111 g          231 t
ORIGIN      
        1 agatgcttaa ttaaaaagta ataagtttta tttgaacgga aataaatctt aatttaaaca
       61 cttgaataga caagtgtaat cattcgtcat agaaaaagac gaggggcgat ttagataaat
      121 tgttgaaagt aatattaaat accttattta tctaatttga ttgaggaaac atgcataggt
      181 gaaaattaga aatacattaa aaatattgaa ttaatttttc gacctacaag ttgaggaaaa
      241 tttaactaga tttgaggatt agcaaagtga aaacaattca attacacaag acaactttga
      301 tattaattgc gatattattg ttttatactt ttatgggtat cttactacca ctcatgcacg
      361 atgatttaca atggtttagc aattataata ctgatatttt aaaagtagga tttgcatcac
      421 ttaatggtcg ctatatcgga aatatctttg aaattatagc tgtacatgta agctggttac
      481 gttggctttc atatggtctc attagtatgg gcatcatttg gatgattatg cacattacac
      541 gttgtaaagc gtggacaagc tattatttgt tagcattttc tttaatgtta attttaccaa
      601 gtgctattta tgcagataca tatggctggt ttgcaggatt t
//
LOCUS       DBNENW010000346          624 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.350, whole genome shotgun sequence.
ACCESSION   DBNENW010000346 DBNENW010000000
VERSION     DBNENW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.350"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            1..516
                     /locus_tag="AC1LW8_002840"
     CDS             1..516
                     /locus_tag="AC1LW8_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIP4774575.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          237 a           77 c          112 g          198 t
ORIGIN      
        1 atgaaaataa aaactgaatt taaagggaac aatataccat atgaatacgc agcaggtgca
       61 gatgtgagtg attctattaa cgggaatcca attaagtcat ttccatttga agtaattgaa
      121 ttaccggaag ggactaaata tcttgcttgg tctttaattg actatgatgc aattcctgta
      181 tgtggctttg cttggattca ttggagtgta gctaatgtaa gtgttagtgg caattcaatt
      241 tctataaaag cagatttatc aagaacaaag ggtgactatg tacaaggtaa aaatagcttt
      301 actagtgggt tgttggctga agatttttca gaaatagaaa atcactatgt aggacctaca
      361 ccacctgatc aagatcatca atatgaatta acagtttatg cgttagatca ttctttaaat
      421 ttgaagaatg ggttctactt gaatgaattt ttaaaagaag taaatcaaca taaaattgat
      481 caaacaagta ttaaccttat aggaagaaaa atttaatact aaatatctca tcaatataaa
      541 attgttcaat taaaagtaca aagaaacaaa ggttttaatt tatatattag gtacggcgtt
      601 cgctataatg caaagaagta atta
//
LOCUS       DBNENW010000347          586 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.351, whole genome shotgun sequence.
ACCESSION   DBNENW010000347 DBNENW010000000
VERSION     DBNENW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.351"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..343
                     /gene="scn"
                     /locus_tag="AC1LW8_002841"
     CDS             <1..343
                     /gene="scn"
                     /locus_tag="AC1LW8_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HIP4774576.1"
                     /translation="RKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNEKLA
                     NELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQKIY
                     NEIDEALKSKY"
     gene            complement(396..>586)
                     /locus_tag="AC1LW8_002842"
     CDS             complement(396..>586)
                     /locus_tag="AC1LW8_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774577.1"
                     /translation="AAPFLMTNADYVWTPMTTVTLFILSLIIFLIFIKTKDVVHLTIF
                     ILNLLFSILYCLPILFYL"
BASE COUNT          258 a           78 c           87 g          163 t
ORIGIN      
        1 tagaaaatct atacttgcgg gaactttagc aatcgtttta gcatcaccac tagtaactaa
       61 tctagataaa aatgaggcac aagctagcac aagcttgcca acatcgaatg aatatcaaaa
      121 cgaaaagtta gctaatgaat taaaatcgtt attagatgaa ctaaatgtta atgaattagc
      181 tactggaagt ttaaacactt attataagcg aactataaaa atttcaggtc aaaaagcaat
      241 gtatgctctt aagtcaaaag actttaagaa aatgtcagaa gcaaaatatc aacttcaaaa
      301 gatttataat gaaattgacg aagcactaaa aagtaaatat taaaaaaacc acccgtaaaa
      361 gggtggtttt aattttctag ataatataaa agtgttcata aataaaacag tataggtaaa
      421 caataaagta ttgaaaaaag taagtttaat atgaaaattg ttaaatgaac gacatctttt
      481 gtttttataa atatcaagaa aataatcaaa ctcaaaataa ataacgtaac tgtagtcata
      541 ggcgtccata cataatcagc attagtcatt aagaatggtg cggcca
//
LOCUS       DBNENW010000348          570 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.352, whole genome shotgun sequence.
ACCESSION   DBNENW010000348 DBNENW010000000
VERSION     DBNENW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.352"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>570
                     /gene="sek"
                     /locus_tag="AC1LW8_002843"
     CDS             <1..>570
                     /gene="sek"
                     /locus_tag="AC1LW8_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HIP4774578.1"
                     /translation="AQGDIGIDNLRNFYTKKDFINLKDVKDNDTPIANQLQFSNESYD
                     LISESKDFNKFSNFKGKKLDVFGISYNGQCNTKYIYGGITATNEYLDKPRNIPINIWI
                     NGNHKTISTNKVSTNKKFVTAQEIDIKLRRYLQEEYNIYGHNGTKKGEEYGHKSKFYS
                     GFNIGKVTFHLNNNDTFSYDLFYTGDDGLP"
BASE COUNT          238 a           76 c           81 g          175 t
ORIGIN      
        1 cgctcaaggt gatataggaa ttgataatct caggaatttt tatacaaaaa aagactttat
       61 aaatttaaaa gatgtaaaag acaatgatac tcctatagct aatcaactac aattttcaaa
      121 tgaatcctat gatttaattt cagaatcaaa agattttaat aaatttagta atttcaaagg
      181 aaaaaaactt gatgtttttg gtattagtta taatggccag tgtaacacta aatacatata
      241 tggtggaatc acagctacta acgaatatct agataaacct agaaatatac ctataaatat
      301 atggatcaat ggaaatcaca aaactatttc taccaataaa gtttcgacaa ataaaaaatt
      361 tgttaccgct caagagattg atatcaaatt aagaaggtac cttcaagaag aatacaacat
      421 ttatggacat aacggcacta aaaaaggaga agaatacggt cataaatcaa aattttattc
      481 tgggtttaat attggtaaag taacgtttca tttaaataat aatgacacct tttcatatga
      541 tttattctac acaggagatg atgggctacc
//
LOCUS       DBNENW010000349          568 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.353, whole genome shotgun sequence.
ACCESSION   DBNENW010000349 DBNENW010000000
VERSION     DBNENW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.353"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..307)
                     /locus_tag="AC1LW8_002844"
     CDS             complement(<1..307)
                     /locus_tag="AC1LW8_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774579.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     "
     gene            complement(438..>568)
                     /locus_tag="AC1LW8_002845"
     CDS             complement(438..>568)
                     /locus_tag="AC1LW8_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774580.1"
                     /translation="ALLSMSYQNIIVYFAWITAIMGFFLVNIALIIDKNIHVILKN"
BASE COUNT          197 a           86 c           63 g          222 t
ORIGIN      
        1 ttagttcttc taattgaaaa tcaaataaat aagtatttct tcttacagta ccaccaagta
       61 acacaatacc cgcgaatatg gtccatccta tcgatttatt tttaactggt ttgacaatat
      121 ttaatttttc ataatcattc ttagtgattt tgacatatgt tttcggtatg agccagttaa
      181 taaatggaaa gaagaagaca atccaattac ttgccaaatc aatcattaaa tattcactat
      241 cgtatttgat tattcgatat ttagggtttt tattaataac tttagattcg caaagcaatg
      301 tctccacatc cctttaattt tatgtgtaat acatttttcg atacttcaaa agacattcaa
      361 atactatcaa gttactgtca tcaaaggttt tattaactga tattttcata tttttaatct
      421 gaatttagta ctttcactta atttttaagt atgacatgaa tgtttttatc tataattaaa
      481 gctatattca ctagaaaaaa acccattatt gctgtaatcc aagcaaaata tactattatg
      541 ttttgataac tcattgataa tagagcat
//
LOCUS       DBNENW010000350          554 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.354, whole genome shotgun sequence.
ACCESSION   DBNENW010000350 DBNENW010000000
VERSION     DBNENW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.354"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT          244 a           72 c           73 g          165 t
ORIGIN      
        1 aaaacaaaac caaaatactg agtcattcaa tcatcaagac attttaacaa gtgatgaaaa
       61 agagttaata aaattacgca aagaaaaaca tcgattgata atgtaaaatg atgatttaaa
      121 gcaagcagtg ctaattttga gacaaaaaat gaagtctttc aacaaaatgc gtatcaatat
      181 tcagtatcag caatgtgcaa agttctaaaa atttcaagaa gtacctatta tgattctaaa
      241 aaaggagaat attacaacta gatacaaaaa gcctcaatca caaccaagta actaaaacgt
      301 aaaaattaat tagattaatt attatctgca taacaaatga ccatatacat caactagaga
      361 cagactattt aatcatttac aatgtcctat tcgttttaga ccatgttcaa atgaatagtg
      421 tttataaaat gttgacactg tagtggcata ttgtaaatta aatattatta tgtgatcatt
      481 ttgtttaaat attagttata agaagtgaaa tattcatttt aaaaggagac ttaaaacttg
      541 aaacgatcaa cgaa
//
LOCUS       DBNENW010000351          550 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.355, whole genome shotgun sequence.
ACCESSION   DBNENW010000351 DBNENW010000000
VERSION     DBNENW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.355"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            344..517
                     /locus_tag="AC1LW8_002846"
     CDS             344..517
                     /locus_tag="AC1LW8_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774581.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT          201 a           52 c           94 g          203 t
ORIGIN      
        1 aaattgtttg aggtgatttc tcggtgtata agacttatca atcagttaaa acatattttt
       61 atagatggtg gggatattaa gttaaaaact taaaatcatc ttatcataaa taacaatctt
      121 aagttagcat tcacgataat agtcattgtt aacattagca tataaggtca tgtcacgttg
      181 aaacagaggt tcctcggcat ttttgaaaat aatcattagc ttgctcacta tataatttga
      241 tgaatatatt tcgtaaaagt gggtatttat ttaatgatta ttctatatat gatagtataa
      301 tgaaatgtag ataggtattt aatttaacag aggtgaaatt gagatgtgga attttattaa
      361 atgtgtgttt aaattcgtat ttagcttagt tgctattaca acattagttg ctggtgttgg
      421 tgtagtagca tttgcttata tctttaaaaa agattttgaa gatattgaaa gaaaaactaa
      481 agaaattatt tctgatattg aaagtaaaaa taactaataa catttagagg ctgggacata
      541 aatccctaaa
//
LOCUS       DBNENW010000352          546 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.356, whole genome shotgun sequence.
ACCESSION   DBNENW010000352 DBNENW010000000
VERSION     DBNENW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.356"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(131..>546)
                     /locus_tag="AC1LW8_002847"
     CDS             complement(131..>546)
                     /locus_tag="AC1LW8_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SA1002 family membrane protein"
                     /protein_id="HIP4774582.1"
                     /translation="LFMGVVFIFIGYISLAISAVIIYGIIQFITIDFGSFFLMGIILI
                     LISSIFQLFIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAVNEKMDINDINIDN
                     FQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT          219 a           88 c           65 g          174 t
ORIGIN      
        1 gtgtctcatc agcacttata cactagtcaa aaaattcatg cctctacttt ttcgaatttc
       61 aaaaataaaa ccacacaaca aaaaagacac catttgaatg ttggtgtctg atggtataat
      121 ttcattgatt ttatttgtaa cttctcattc tatagttaaa tattgtgacc cagctaatga
      181 ttaacgttgg tagaattact aaattcaaat tagacacgtc aaaaaagaca gattgaaaat
      241 tgtcgatatt aatatcatta atgtccattt tttcatttac tgcttgatag atcgcaaagt
      301 aaactaagaa ccattgaatt aaatgttcta aaacgacaac ctctgtcaaa tcgacattct
      361 tttttctaaa aagtaatcta actataaata attggaatat acttgaaatc aagattaata
      421 taatacccat taagaaaaaa ctaccaaaat caattgtgat aaattgaata atgccataaa
      481 taattacggc agatattgcc agtgaaatat atcctataaa gataaatacc acacccataa
      541 agagtg
//
LOCUS       DBNENW010000353          545 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.357, whole genome shotgun sequence.
ACCESSION   DBNENW010000353 DBNENW010000000
VERSION     DBNENW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.357"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT          228 a           81 c           68 g          168 t
ORIGIN      
        1 ataaaaacta aagtaaaaat gttggatgtg aaagattgtt gatgagaatt ttgagatgta
       61 gataaatatc aaactagtct ataggtaaaa atttataatt aatttgaaaa gaatgtctca
      121 aataatgttt gaattagcag gtgtcatact aagtaatgtt aagtaaacat tttatacttt
      181 atcagaacaa aaaagacatg catcaacatg tcagtgaaac taatccatat tatcttaaca
      241 acgaaattga atcgaacctg aaaaacgtat ttacaaatcg caactactct atccaaggca
      301 aaatcgcaaa atctgatccc cgaaataaca tatctcgaaa aaaattccat aattcaatca
      361 tcttcaatat ttaacaatac attactagtt ccaaatatac tcatacatac atgtttcatt
      421 gattgactgc atattgtcat aaataatagg ggaactaatc gctaataatg ttgttaaaca
      481 caatacatag taaagtgaaa gattataaat acatgaattg ttcaccattc cgccatatat
      541 tttca
//
LOCUS       DBNENW010000354          544 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.358, whole genome shotgun sequence.
ACCESSION   DBNENW010000354 DBNENW010000000
VERSION     DBNENW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.358"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            261..544
                     /locus_tag="AC1LW8_002848"
                     /pseudo
     CDS             261..544
                     /locus_tag="AC1LW8_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          221 a           78 c           77 g          168 t
ORIGIN      
        1 ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa tgtaaaattc
       61 aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac tactaaacta
      121 aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca ctaccaatag
      181 aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa attgttgaaa
      241 cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga ataggtttta
      301 aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag ctatatgaat
      361 gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat tcgaaatcca
      421 ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt aatcaaaagc
      481 tttctgaacc agaaacgaaa aaatctatag tcaaagaaaa attgatgtag agcctgtttt
      541 tgga
//
LOCUS       DBNENW010000355          530 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.359, whole genome shotgun sequence.
ACCESSION   DBNENW010000355 DBNENW010000000
VERSION     DBNENW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.359"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>530
                     /locus_tag="AC1LW8_002849"
     CDS             <1..>530
                     /locus_tag="AC1LW8_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774583.1"
                     /translation="ITDIKNSEKKDQDYIEYKRKSPAQIILMIQRNLFALQMNPVIFF
                     IINFILISYLYDKQYVQFQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIM
                     GTIIAIISLLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGL
                     LFCQKTVDAMIHNPQSA"
BASE COUNT          181 a           72 c           74 g          203 t
ORIGIN      
        1 attactgata taaaaaattc tgaaaagaaa gatcaagatt atattgaata taaaagaaaa
       61 tctccagcac aaatcatatt aatgattcaa aggaatttgt ttgctttaca aatgaatcca
      121 gttattttct ttattataaa cttcattctc atatcctatt tatatgataa acagtatgtt
      181 cagtttcaag ctattactgg aatgagtcta ttttattgtt tagtgatttt tccaatgact
      241 attgttgtat acctaagggt gtcacaaaag aattacttgc gtagtaataa aatagaaatg
      301 attatgggta caattatcgc tatcatatcc ttgttattaa ttatattaca agcatttaat
      361 attacttggg gcgttatacc aattacaaat tttggacatc aatttttctt tttcattggt
      421 attattttag taattgccgg catattttat aagcgacttg agttttcggg aatcggctta
      481 ttattttgtc aaaaaaccgt cgatgcaatg attcataatc cacaatcagc
//
LOCUS       DBNENW010000356          520 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.361, whole genome shotgun sequence.
ACCESSION   DBNENW010000356 DBNENW010000000
VERSION     DBNENW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.361"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..213)
                     /locus_tag="AC1LW8_002850"
     CDS             complement(<1..213)
                     /locus_tag="AC1LW8_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIP4774584.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGD"
BASE COUNT          178 a           74 c           67 g          201 t
ORIGIN      
        1 atctccttta atgaaccata ataattcggt tgctactaat ttaaaagaaa ctttctttgt
       61 cgttaatagt ggaaatcctt tagataagtc aaagcgaagt tgatgaccaa atttcgaaat
      121 cgtacctgta tttgtgcgat catttcgtgt atttcctatt tctaaaactt cttcacaaag
      181 actgtgatat gctgcatcaa atgaattcaa catatgcgat aacacctcat tttcattatt
      241 tatagtatgt atatttagtt tgatataact taattttatg tagcattttg ttatcactca
      301 ttttaggaat atgatattaa tatcatgaat tccgttactt tatttataaa atgctgatta
      361 agtacctacc acatcgtaac gtgatatatg tttccaattg gtaattgttt acccaaatct
      421 ataactttaa tgctaaaaaa ttttaaaaaa gaggttaaca catgatttga atattatgtt
      481 tgatgtccta ttaaaacagt taaatttcta gaaaatatag
//
LOCUS       DBNENW010000357          519 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.362, whole genome shotgun sequence.
ACCESSION   DBNENW010000357 DBNENW010000000
VERSION     DBNENW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.362"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            92..289
                     /locus_tag="AC1LW8_002851"
     CDS             92..289
                     /locus_tag="AC1LW8_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774585.1"
                     /translation="MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLK
                     LVSHNVYMLSTVLYPNWRLLT"
BASE COUNT          207 a           62 c           70 g          180 t
ORIGIN      
        1 aaaatataaa ttcgatttaa taattaattt aaatttagtt aatcaatttt gcatctattt
       61 tgttgtaagc tatataaaag gagtgataat gatggtgaaa aaaacaaaat ccaattcact
      121 aaaaaaagtt gcaacacttg cattagcaaa tttattatta gttggtgcac ttactgacaa
      181 tagtgccaaa gccgaatcta agaaagatga tactgatttg aagttagtta gtcataacgt
      241 ttatatgtta tcgaccgttt tgtatccaaa ctggagactt ttaacataaa attacttatc
      301 attcaaaaag taaaacagca taatatcaag gtttataact ttatcattat caataatacc
      361 tcatataaaa taaaatttta gggacttttt agggacttta aatttaaaat tacaagttta
      421 atagaaacat caaaataatc acatgtttgt gtggaatgta caccccaaaa gctagactga
      481 aaaatctatt ttttgaggtg tatttttata ggtaaatat
//
LOCUS       DBNENW010000358          515 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.363, whole genome shotgun sequence.
ACCESSION   DBNENW010000358 DBNENW010000000
VERSION     DBNENW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.363"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            93..>515
                     /locus_tag="AC1LW8_002852"
     CDS             93..>515
                     /locus_tag="AC1LW8_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774586.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKADELSNE
                     GMVLYLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMK"
BASE COUNT          230 a           42 c          100 g          143 t
ORIGIN      
        1 tttgataaca taaaagttga ataatcacta ttttaataag ttatatgaaa tattcaaatg
       61 tttataaaat taaagcagag agatgtgaaa tgatggagta tataaaaaaa attgctttgt
      121 acatgagtgt attactttta atcattttta ttgggggatg tggaaatatg aaagatgaac
      181 agaaaaaaga ggaacaaacg aataaaacag attcaaaaga agaacaaatc aaaaagagtt
      241 ttgcgaaaac gttagatatg tatccaatta agaatctcga ggatctatat gacaaagaag
      301 gatatcgaga tggtgagttt aaaaaaggtg ataaaggtat gtggacgata tatacagatt
      361 ttgccaaaag taataaagca gacgaattaa gtaatgaagg tatggtctta tacttagata
      421 gaaatacacg gacagcaaag ggacattatt ttgttaagac attttatgaa aaggataaat
      481 tcccagatag aaaaaaatat aaagttgaaa tgaaa
//
LOCUS       DBNENW010000359          498 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.365, whole genome shotgun sequence.
ACCESSION   DBNENW010000359 DBNENW010000000
VERSION     DBNENW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.365"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(140..340)
                     /gene="cspA"
                     /locus_tag="AC1LW8_002853"
                     /gene_synonym="msaB"
     CDS             complement(140..340)
                     /gene="cspA"
                     /locus_tag="AC1LW8_002853"
                     /gene_synonym="msaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HIP4774587.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          171 a           93 c           66 g          168 t
ORIGIN      
        1 caaacaatgt tggtgatata aaaaaagagt tctcaatttg gctgagaaac tcatcgcgtt
       61 aagtatttgt aataagcata taaaaacacc ttacttcttg gtaaggtgtt agttaaaatc
      121 aatgattcaa atctaagaat tatagtttaa caacgtttgc agcttgtgga ccgcggtcgc
      181 cttcaactac ttcaaactca acagcttgac cttcttctaa tgatttgtaa ccatcttggt
      241 taattgctga aaaatgtacg aatacgtcat tttctccttc aacttcgata aagccgaatc
      301 ctttttcagc gttaaaccat ttaactgtac cttgtttcat aatctgaaac ctccaagact
      361 aaaattcatt caatatgctt attcgcatat tacaaaaaat acatgcaaat ttcactcaaa
      421 tattctttgc aatatggaat aatcatctgc ttaacttgta ttatagtgca gttattacca
      481 aattgcaata caataata
//
LOCUS       DBNENW010000360          483 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.366, whole genome shotgun sequence.
ACCESSION   DBNENW010000360 DBNENW010000000
VERSION     DBNENW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.366"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..91
                     /locus_tag="AC1LW8_002854"
     CDS             <1..91
                     /locus_tag="AC1LW8_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774588.1"
                     /translation="SNPINKSFEKSGASEKLKSTLSEKAKKKD"
     gene            complement(88..>483)
                     /locus_tag="AC1LW8_002855"
     CDS             complement(88..>483)
                     /locus_tag="AC1LW8_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIP4774589.1"
                     /translation="GLKANILFLFIFVLTVFVFNPLIVKFIIWLINITRKFMKLDCIS
                     LLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDFDLLEVERQDFKVSDL
                     PSNDELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT          188 a           74 c           57 g          164 t
ORIGIN      
        1 tagtaaccca ataaacaaaa gttttgaaaa gtcaggcgcc agcgaaaaat taaaaagcac
       61 tttatcagaa aaagctaaga aaaaagatta gtattcattc attaaatata aatccaattt
      121 aatttgttgt ttaaggtcta caagcgtatg tttaatatac aattcatcgt ttgacggtaa
      181 atcagatact ttgaaatctt gtcgctcaac ttctagtaaa tcgaaatcgc taccagctga
      241 attataggtt ttaagttcac cctcttcaat gattctgttt tcaaagtctt ttataactat
      301 aaatactggt ttaccgttgt tattaaacaa cttgtctctt ttgtctaata agcttataca
      361 atccaatttc ataaactttc tggttatatt aattaaccag ataataaatt taacaattaa
      421 aggattaaat acaaacactg ttaaaacaaa aataaataga aacaaaatat ttgcttttag
      481 acc
//
LOCUS       DBNENW010000361          459 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.368, whole genome shotgun sequence.
ACCESSION   DBNENW010000361 DBNENW010000000
VERSION     DBNENW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.368"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            167..>459
                     /locus_tag="AC1LW8_002856"
     CDS             167..>459
                     /locus_tag="AC1LW8_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774590.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEY"
BASE COUNT          178 a           53 c           77 g          151 t
ORIGIN      
        1 gataggtaat gaaaaagttc gcgttataat gaaaaataat tgattacatt taaaatattc
       61 taaatgttgt cgacacaatc cttttaagac gctagtagaa tttaaatgac ttctaatgta
      121 tatgaaagtg tatcaatata aaaccaattg aaaagaagtg gagacattgc tttgtgaaac
      181 tgaaaatatt aataagaatc ccaaatatag aattatcaaa tacaaagatg aatatttgat
      241 gattgattta gtaagtacat ggttagcact ctttttccca atgattaatt ggctgattcc
      301 aaaaaagtac gtcaaaatca gcgaaaaaga ttttgaaact ttaaacattg tgaagacagc
      361 taaaatcaat tctttttggc cagtggcagg aagtacggtc ttatttggtg ttatgttaag
      421 aaggtattcc catttattta tcgttaaata tgaatatag
//
LOCUS       DBNENW010000362          445 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.369, whole genome shotgun sequence.
ACCESSION   DBNENW010000362 DBNENW010000000
VERSION     DBNENW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.369"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            82..>445
                     /locus_tag="AC1LW8_002857"
     CDS             82..>445
                     /locus_tag="AC1LW8_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HIP4774591.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRK"
BASE COUNT          165 a           43 c           71 g          166 t
ORIGIN      
        1 aaagttcatg tattatttaa atcaaatgga attgaaaagt tttaatagga ataataaaca
       61 aaggaatggt agtgataaat attgctatgt gaatctaaag ttatcaacaa aaatcctaag
      121 tatagagtta ttaaatatgg tgatgaatat ttaatgattg atttagtaag tacctggtta
      181 actttatttc ttcctatgat taattggtta attccaaaaa aatatgtcaa aatcagtaaa
      241 aaagaatttg acgatttaaa cattgtcaaa cctgttaaaa ataaagcttt ttggccagtt
      301 gcaggtagta ctattttgtt cggagttacg tttagaaaat atattccttc acttaatatt
      361 caattagaga aaaacatggt gattgtaata tgttgtgcaa tatttctggg tgttttaata
      421 ctttttttat ttctgaatcg taagc
//
LOCUS       DBNENW010000363          426 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.370, whole genome shotgun sequence.
ACCESSION   DBNENW010000363 DBNENW010000000
VERSION     DBNENW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.370"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            24..344
                     /locus_tag="AC1LW8_002858"
     CDS             24..344
                     /locus_tag="AC1LW8_002858"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP4774592.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYKDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
BASE COUNT          156 a           61 c           81 g          128 t
ORIGIN      
        1 aactatctag aggagttgtt acaatgcaag caatcaaaag taatgaatca tttaaatctg
       61 tgattaatag cgatacacct gtaattgtta aatttgaggc aggatggtgc ccagactgtc
      121 gtgctatgga tttatggatt gacccaatcg tagaacaata taaagattac caatggtata
      181 ctgttaatcg tgatgaatta gaagatgtag ttgttgaaaa tgaagttatg ggtatcccta
      241 gcttgctagt atttaaaaac ggcgacaaaa ttgcacacct tcattcagca aatgctaagt
      301 cacctgagca agttgaatca tttttagcag aaacttttaa ataaatacta aaataaaaat
      361 gtgtatatac tgcacaataa aaagctgaga ctgtgattgt accgactcta gtaagttaga
      421 ttaaat
//
LOCUS       DBNENW010000364          420 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.371, whole genome shotgun sequence.
ACCESSION   DBNENW010000364 DBNENW010000000
VERSION     DBNENW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.371"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT          176 a           51 c           61 g          132 t
ORIGIN      
        1 aatctccatt agaactaaga tgtgcatccc gaaagttaga ctaaaaaact aactttatgg
       61 gatgtatttt tatgctaatc atcataaatt cgagattaag ttaaaggtag ttcaagagta
      121 attaaacaat aaattaaaaa tagtaggata cttactttga gggaagaaaa ttaactgtat
      181 atatttagtt taggaacaag tattacggtt tatcctgata caattattgt ggatgggatg
      241 atatttttag gtttaaaata cgacaccagc aaacataata actgtaatag ctcataaatc
      301 tccccatata gctaatctaa aaaaataata catcattgga attaagcccc aagcatgtaa
      361 atattaaaaa tcaaaaaaga tatatgtaaa aaagttacaa tttgcataat taaattgtgt
//
LOCUS       DBNENW010000365          414 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.372, whole genome shotgun sequence.
ACCESSION   DBNENW010000365 DBNENW010000000
VERSION     DBNENW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.372"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..>414)
                     /locus_tag="AC1LW8_002859"
     CDS             complement(<1..>414)
                     /locus_tag="AC1LW8_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774593.1"
                     /translation="YLSSTNINFSLPISIKAITTIQLKHWISNKEEVNLHKPFFYISY
                     SIWFFLGCLIYFSQMAYNQLANAVIFILILNTITRDSFSAGTDFFTKSLRFDSDRKSI
                     GLYRMSNTDFKQIYDSKLSLIRFIGFKETILAIIL"
BASE COUNT          165 a           53 c           56 g          140 t
ORIGIN      
        1 aataaaataa tagcaagtat tgtttcttta aaaccaataa agcgaattag agataattta
       61 gaatcgtaaa tttgtttaaa atcagtgttt gacatcctat acaaaccgat actttttcta
      121 tctgaatcaa aacgtaatga tttagtaaaa aaatccgtcc ccgcagaaaa actatcacga
      181 gttatagtgt ttaatatcaa aataaaaata actgcattag ctaactgatt atatgccatc
      241 tgtgaaaaat agattaaaca ccctaaaaag aaccatattg aataactaat atagaagaaa
      301 ggtttatgta aattaacttc ttctttgtta cttatccagt gttttagctg tattgttgtt
      361 atagctttta ttgaaattgg caacgaaaaa ttaatatttg ttgaagatag atag
//
LOCUS       DBNENW010000366          409 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.373, whole genome shotgun sequence.
ACCESSION   DBNENW010000366 DBNENW010000000
VERSION     DBNENW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.373"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT          138 a           61 c           63 g          147 t
ORIGIN      
        1 ttttttatta gtttctcacg gcggattgtg acaatgatat ttaacacaat gatgaggtct
       61 ctattccaat aataaaatta ggataatggc aacttctagt tgcacgtcat tgctcagaaa
      121 ataaatatca ttattcgtta ttcgttactt taacaattga tgttatggag gactggtggc
      181 atatcacaac gaagaaatat gttgacagta gcatatgttt gtacgtctaa taaagatttt
      241 attgtctacg tttatctttc atatccaacc actaagttac ttgaggttca tacattttaa
      301 agaaaatgga cgaacagata aatcaatatg attatcattc gtccatatat cttttattaa
      361 tataatatta aacgcaaaaa aatgcctatc ctcttggata ggcatttgt
//
LOCUS       DBNENW010000367          407 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.374, whole genome shotgun sequence.
ACCESSION   DBNENW010000367 DBNENW010000000
VERSION     DBNENW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.374"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            212..367
                     /locus_tag="AC1LW8_002860"
     CDS             212..367
                     /locus_tag="AC1LW8_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774594.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
BASE COUNT          158 a           32 c           65 g          152 t
ORIGIN      
        1 gtaatatatc gaaagattta aatgtaaaat tttgatttgt taagaaatta cgtttgtaaa
       61 aataaaaaaa tcaacttatt tgtatgagat aaatatgtat tgaagaagaa gtgttattaa
      121 tttggaaaat actggtcaaa gatgggagct ctcaaaagcg ttattgtatt ttttagtcaa
      181 tacaaataga ttgccgtaat aataatcgta cttgatggtt aaaaaattac ttaaggctat
      241 aaagcaaaac tttttatatg agcagtcgaa tataacgttt aaaatgattg tttttggata
      301 taaacgatta agtaaaatgc tttttcagtt tgaaattaat catataaatt tcttatggga
      361 aggttgatat cttaatgatt aatattattt cagctatagg atctatt
//
LOCUS       DBNENW010000368          403 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.376, whole genome shotgun sequence.
ACCESSION   DBNENW010000368 DBNENW010000000
VERSION     DBNENW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.376"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            75..155
                     /gene="hld"
                     /locus_tag="AC1LW8_002861"
     CDS             75..155
                     /gene="hld"
                     /locus_tag="AC1LW8_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-hemolysin"
                     /protein_id="HIP4774595.1"
                     /translation="MAQDIISTISDLVKWIIDTVNKFTKK"
BASE COUNT          147 a           47 c           60 g          149 t
ORIGIN      
        1 gatgtgatgg aaaatagttg atgagttgtt taattttaag aatttttatc ttaattaagg
       61 aaggagtgat ttcaatggca caagatatca tttcaacaat cagtgactta gtaaaatgga
      121 ttatcgacac agtgaacaaa ttcactaaaa aataagatga ataattaatt actttcattg
      181 taaatttgtt atcttcgtat agtactaaaa gtatgagtta ttaagccatc ccaacttaat
      241 aaccatgtaa aattagcaag tgagtaacat ttgctagtag agttagtttc cttggactca
      301 gtgctatgta tttttcttaa ttatcattac agataattat ttctagcatg taagctatcg
      361 taaacaacat cgatttatca ttatttgata aataaaattt ttt
//
LOCUS       DBNENW010000369          403 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.377, whole genome shotgun sequence.
ACCESSION   DBNENW010000369 DBNENW010000000
VERSION     DBNENW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.377"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>403
                     /locus_tag="AC1LW8_002862"
     CDS             <1..>403
                     /locus_tag="AC1LW8_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774596.1"
                     /translation="KKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKKDKGTWIINSEM
                     ATQNKGEALKIKGMVLYMNRNTKTTKGYYYVNAIKNDKDGRPQENEKRYPVKMIDNKV
                     IPTKEIKDENIKTEIKNFKFFVQYGNFKDLK"
BASE COUNT          194 a           39 c           67 g          103 t
ORIGIN      
        1 ttaaaaaaag ttttgaaaaa acgttgagta tgtatccgat taaaaatcta gaagatttat
       61 acgataaaga aggatatcgt gatgatcaat ttgataaaaa agataaaggc acatggatta
      121 ttaattctga aatggcgact caaaataagg gagaagcttt gaaaattaaa ggtatggtct
      181 tatacatgaa tagaaataca aaaacaacaa aaggatatta ttatgtaaat gcaataaaga
      241 atgataaaga cggaagaccc caagagaatg aaaaaaggta cccagttaaa atgattgata
      301 ataaagttat tccaacaaaa gaaattaaag atgaaaacat aaaaacagaa atcaaaaact
      361 ttaagttctt tgttcaatat ggcaacttta aagatttgaa aaa
//
LOCUS       DBNENW010000370          377 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.378, whole genome shotgun sequence.
ACCESSION   DBNENW010000370 DBNENW010000000
VERSION     DBNENW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.378"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..95
                     /locus_tag="AC1LW8_002863"
     CDS             <1..95
                     /locus_tag="AC1LW8_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774597.1"
                     /translation="VGKTKIKEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          155 a           43 c           51 g          128 t
ORIGIN      
        1 aggtaggcaa aactaaaata aaagaagcta aatttaaaac gataatcaag ttatatgaat
       61 atcaaagaac attagaaaat aaaacagaat gttaacaaaa atattttgtt aggttaggtg
      121 gacaagaaat taaatttcta atgatttcat ggttgtctgt ccacgttttg tccacctcaa
      181 taagataatc tcttagtttt agaaattgta catggttaaa taatcaaaaa tgcatattat
      241 gaaattatca ttgccattaa atcttgaatg atctttaagc gatgatataa aaggctgaac
      301 acagtaaata agttaacttt aatacagtat tctatactat acatattttg taataatgtt
      361 tatgcacata atttttc
//
LOCUS       DBNENW010000371          372 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.379, whole genome shotgun sequence.
ACCESSION   DBNENW010000371 DBNENW010000000
VERSION     DBNENW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.379"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..349
                     /locus_tag="AC1LW8_002864"
     CDS             <1..349
                     /locus_tag="AC1LW8_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HIP4774598.1"
                     /translation="AIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYLEEKNLK
                     DKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETKKSLKTA
                     KHKYFNQNYKPSK"
BASE COUNT          153 a           45 c           59 g          115 t
ORIGIN      
        1 cgcgattaaa gttatcatta tattatttat ttcattaatt gttgcagtag catttttctt
       61 tggtctgaaa acatatcaag gccataagaa tattcaacta attgacagtt acttggaaga
      121 gaaaaatcta aaagataaaa ttaaatcaga aaaaacagag tatagtgcta aaaaaggtct
      181 cttctataag gaagttacat ttaaagatga acctggtgta acttatgttg tgcaaccaat
      241 cagtactaat aaaggattat ttgttgaagg ctttgataca gagactaaga aaagtttgaa
      301 aactgcaaag cataaatatt ttaatcaaaa ttataaacca tcaaaatagt gtaacgtata
      361 actggtgaaa ca
//
LOCUS       DBNENW010000372          372 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.380, whole genome shotgun sequence.
ACCESSION   DBNENW010000372 DBNENW010000000
VERSION     DBNENW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.380"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(<1..361)
                     /locus_tag="AC1LW8_002865"
     CDS             complement(<1..361)
                     /locus_tag="AC1LW8_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HIP4774599.1"
                     /translation="MGYLKGFALYISILILIVFIAGCGKSDKTKEDSKEAQIKKSFEK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFAKSNKPGEIDDEGMVLFLNR
                     NIKKATGYYYTSKVHDEF"
BASE COUNT          104 a           78 c           37 g          153 t
ORIGIN      
        1 taaattcatc atgaactttg ctcgtgtaat aatatcctgt tgccttcttt atatttctat
       61 ttaaaaataa aaccatgcct tcatcatcta tctcacctgg tttgttactt ttagcaaaac
      121 ttgtgagtaa agtccatgta cccttatcac cctttttaaa ttcaccatct ctatagcctt
      181 ctttgtcgta taaatcctcg agatttttga ttggatacat atctaaagtt ttctcaaagc
      241 tctttttaat ttgtgcttct tttgaatctt cttttgtttt atcacttttg ccacaacctg
      301 ctataaaaac tattaaaatt aagatactta tgtacaatgc aaaccctttt aaatatccca
      361 tcgtttcacg tc
//
LOCUS       DBNENW010000373          371 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.381, whole genome shotgun sequence.
ACCESSION   DBNENW010000373 DBNENW010000000
VERSION     DBNENW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.381"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..354
                     /locus_tag="AC1LW8_002866"
     CDS             <1..354
                     /locus_tag="AC1LW8_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP4774600.1"
                     /translation="TKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPDNPG
                     LSKDRAKPNEPVHSMDKNDKVKKSKIAKKSVANQEKKRAELPKTGAESTQKGLIFSSI
                     IGIAGLMLLARRRKN"
BASE COUNT          168 a           59 c           68 g           76 t
ORIGIN      
        1 acaaaacaac cagtagataa gattgtagag tttggtggcg agaaaccaaa agatccaaaa
       61 ggacctgaaa acccagagaa gccgagcaga ccaactcatc caagtggccc agtaaatcct
      121 gacaatccag gattatcgaa agacagagca aaaccaaatg aaccagttca ttcaatggat
      181 aaaaatgata aagttaaaaa atctaaaatt gctaaaaaat cagtagctaa tcaagagaaa
      241 aaacgagcag aattaccaaa aacaggtgca gaaagcacac aaaaaggatt gatctttagt
      301 agtataattg gaattgctgg attaatgtta ttggcacgta gaagaaaaaa ttaaaataat
      361 tcattattta a
//
LOCUS       DBNENW010000374          339 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.382, whole genome shotgun sequence.
ACCESSION   DBNENW010000374 DBNENW010000000
VERSION     DBNENW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.382"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..>339
                     /locus_tag="AC1LW8_002867"
     rRNA            <1..>339
                     /locus_tag="AC1LW8_002867"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          107 a           66 c           93 g           73 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaaggacg acattagacg aatcatctg
//
LOCUS       DBNENW010000375          333 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.384, whole genome shotgun sequence.
ACCESSION   DBNENW010000375 DBNENW010000000
VERSION     DBNENW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.384"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..88
                     /locus_tag="AC1LW8_002868"
     CDS             <1..88
                     /locus_tag="AC1LW8_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP4774601.1"
                     /translation="TAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            105..322
                     /locus_tag="AC1LW8_002869"
                     /pseudo
     CDS             105..322
                     /locus_tag="AC1LW8_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020813.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T7SS effector LXG polymorphic toxin"
BASE COUNT          125 a           40 c           68 g          100 t
ORIGIN      
        1 gactgcaggt ttctttctgt taaacatgtc atcgattatt gacaaaaaaa tttatgtatt
       61 atctaaaact aacacggtgg aaaaatgatg gtttagctgg atttactgca ggttctattt
      121 cggcaatact tgtatattgg accaatcaaa aaaatgaatt tggaataaaa gataaaaacg
      181 attggatagg acataaacta gacgttggta tagatgctgt agaaaaatct gcagaaaaaa
      241 cagtagatgg tgttgaaaat gtcatggtga agcttcaaaa agtatttcta atcatataag
      301 ccctaagaaa tggagctggt aaatgttgct atg
//
LOCUS       DBNENW010000376          328 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.385, whole genome shotgun sequence.
ACCESSION   DBNENW010000376 DBNENW010000000
VERSION     DBNENW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.385"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(77..>328)
                     /gene="sarT"
                     /locus_tag="AC1LW8_002870"
     CDS             complement(77..>328)
                     /gene="sarT"
                     /locus_tag="AC1LW8_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HIP4774602.1"
                     /translation="NYLVLAYLDVFKNDEGKYFMRDIISYIGIDQSRIVKSVKELSKK
                     GYLNKCRDPHDSRNVIIVVSVKQHNYIKNLLSEININET"
BASE COUNT          120 a           52 c           24 g          132 t
ORIGIN      
        1 tctaaaataa aaaatacctt aacgcattta aaaatacatt taactgcacc aagttattaa
       61 aatctcgaaa catactttat gtttcattaa tatttatttc actcaaaaga tttttaatat
      121 aattgtgttg ttttacagaa acaacaatga ttacatttct actatcatgt gggtctctac
      181 atttattcaa gtaacccttt tttgataatt cttttacgct tttaacaatt ctagattggt
      241 ctataccgat ataacttata atgtctctca taaaatattt accttcatca tttttaaata
      301 catctaaata tgctaatact aaataatt
//
LOCUS       DBNENW010000377          295 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.386, whole genome shotgun sequence.
ACCESSION   DBNENW010000377 DBNENW010000000
VERSION     DBNENW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.386"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT          122 a           32 c           53 g           88 t
ORIGIN      
        1 tcaaaataaa agttaacaca aagaaaatgg cttgacgaag tgaaaacgtt tgaatctgac
       61 gaaacgagaa aagagcgcaa cgagtttagt agagctaaat gagtaagcga gagccgaaga
      121 aaatgataat ttaataatgt acactttcga ttgtctaagt atgtataact ttaattttgt
      181 gtttatataa atttaaaatg atatcatcga aaacaaaata ttgtataaat agagaagagc
      241 agtaagacgg tatctaattg aaaatgatct tactgctctt ttatatactt tattg
//
LOCUS       DBNENW010000378          288 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.387, whole genome shotgun sequence.
ACCESSION   DBNENW010000378 DBNENW010000000
VERSION     DBNENW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.387"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            57..139
                     /locus_tag="AC1LW8_002871"
     tRNA            57..139
                     /locus_tag="AC1LW8_002871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Leu,seq:gag)
     gene            161..234
                     /locus_tag="AC1LW8_002872"
     tRNA            161..234
                     /locus_tag="AC1LW8_002872"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Gly,seq:tcc)
BASE COUNT           89 a           57 c           64 g           78 t
ORIGIN      
        1 acataatata ataaataacg ttctgaaaaa cgaagatgtt ttaaatgcct atatccgcgg
       61 tcgtggcgga atggcagacg cgctaggttg agggcctagt gggagaaatc ccgtggaggt
      121 tcaagtcctc tcggccgcat caaaattctt aattaaataa gcgggtgtag tttaatggca
      181 aaacctcagc cttccaagct gatgttgtgg gttcgattcc catcacccgc tccatagata
      241 attttaatga acattgaaaa ctgaatgaca atatgtcaac gttaattc
//
LOCUS       DBNENW010000379          278 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.390, whole genome shotgun sequence.
ACCESSION   DBNENW010000379 DBNENW010000000
VERSION     DBNENW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.390"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(24..>278)
                     /locus_tag="AC1LW8_002873"
     rRNA            complement(24..>278)
                     /locus_tag="AC1LW8_002873"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DBNENW010000380          278 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.391, whole genome shotgun sequence.
ACCESSION   DBNENW010000380 DBNENW010000000
VERSION     DBNENW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.391"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            24..245
                     /locus_tag="AC1LW8_002874"
     CDS             24..245
                     /locus_tag="AC1LW8_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HIP4774603.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
BASE COUNT           81 a           41 c           72 g           84 t
ORIGIN      
        1 tttgagaagg aggtggaagg cctatgctaa aattacataa aaaaatagct tggacaggaa
       61 tcaaaggttc agctataact acatttatta ctgccttata taatggaagt agtgtttgga
      121 acgcgctagc tgttgctggt attgcttttg gtggtggcgc tggtactgcg gttgcagctc
      181 ttggtcgtgc aacagtaatg agatttatca aacgttgggg cgtacgaaaa acagctgctt
      241 ggtaaaagat aatctaattc tttataagag aggctttc
//
LOCUS       DBNENW010000381          276 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.392, whole genome shotgun sequence.
ACCESSION   DBNENW010000381 DBNENW010000000
VERSION     DBNENW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.392"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
BASE COUNT           76 a           59 c           46 g           95 t
ORIGIN      
        1 ttcttttcga aattctctgt gttggggccc acaccccaac tcgcattgcc tgtagaattt
       61 cttttcgaaa ttctctgtgt ttgggcccct gactagagtt gaaaaaagct tgttgcaagc
      121 gcattttcat tcagtcaact actgccaata taatattata gaccctagga cattgattta
      181 tctcccaagc tccatcagta cgatttatta tagaacccgt tttaagtaat tagagagacg
      241 agaatcattt tgtaaatttc gtcagttcaa aacttt
//
LOCUS       DBNENW010000382          271 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.denovo.393, whole genome shotgun sequence.
ACCESSION   DBNENW010000382 DBNENW010000000
VERSION     DBNENW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.denovo.393"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..270
                     /locus_tag="AC1LW8_002875"
     CDS             <1..270
                     /locus_tag="AC1LW8_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HIP4774604.1"
                     /translation="DWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDKLDRNFEELRR
                     DKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
BASE COUNT          119 a           26 c           56 g           70 t
ORIGIN      
        1 gattggcgtt taacgcgatt agaagaaaat gataagacta tgtttgaaaa attcgacaga
       61 atagaagaca gtctgagaac gcaagaaaaa atttatgaca agttagatag aaatttcgaa
      121 gaactaaggc gtgacaaaga agaagatgaa aaaaataaag agaaaaatgc taaaaatatt
      181 agagacatca agatgtggat tctaggatta atagggacga ttctaagtac atttgttata
      241 gccttgttaa aaactatttt tggcatttaa a
//
LOCUS       DBNENW010000383         9955 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.guided.094, whole genome shotgun sequence.
ACCESSION   DBNENW010000383 DBNENW010000000
VERSION     DBNENW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.guided.094"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            complement(123..416)
                     /locus_tag="AC1LW8_002876"
     CDS             complement(123..416)
                     /locus_tag="AC1LW8_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HIP4774605.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            complement(830..1363)
                     /locus_tag="AC1LW8_002877"
     CDS             complement(830..1363)
                     /locus_tag="AC1LW8_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP4774606.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            complement(1732..2577)
                     /gene="blaZ"
                     /locus_tag="AC1LW8_002878"
     CDS             complement(1732..2577)
                     /gene="blaZ"
                     /locus_tag="AC1LW8_002878"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HIP4774607.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            2684..4441
                     /gene="blaR1"
                     /locus_tag="AC1LW8_002879"
     CDS             2684..4441
                     /gene="blaR1"
                     /locus_tag="AC1LW8_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HIP4774608.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNGQ"
     gene            4431..4811
                     /gene="blaI"
                     /locus_tag="AC1LW8_002880"
     CDS             4431..4811
                     /gene="blaI"
                     /locus_tag="AC1LW8_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HIP4774609.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5075..5668
                     /locus_tag="AC1LW8_002881"
     CDS             5075..5668
                     /locus_tag="AC1LW8_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP4774610.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            5640..7083
                     /locus_tag="AC1LW8_002882"
                     /pseudo
     CDS             5640..7083
                     /locus_tag="AC1LW8_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
     gene            7076..7891
                     /locus_tag="AC1LW8_002883"
     CDS             7076..7891
                     /locus_tag="AC1LW8_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP4774611.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(8187..8606)
                     /locus_tag="AC1LW8_002884"
     CDS             complement(8187..8606)
                     /locus_tag="AC1LW8_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP4774612.1"
                     /translation="MELDNCINYLLSISQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNNYFANLTNEETVVFKKILLKINETTF"
     gene            8730..9701
                     /locus_tag="AC1LW8_002885"
     CDS             8730..9701
                     /locus_tag="AC1LW8_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIP4774613.1"
                     /translation="MFNAVVVRKSASNVSYKVETVDESYLSKGDVLVKVVYSSINYKD
                     MLALQYNGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILRKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNASILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(9873..>9955)
                     /locus_tag="AC1LW8_002886"
     CDS             complement(9873..>9955)
                     /locus_tag="AC1LW8_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIP4774614.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
BASE COUNT         3680 a         1365 c         1508 g         3402 t
ORIGIN      
        1 aattaataat aaagtactaa aatctaaaaa taatttgaat tttttcatat tgcctctttc
       61 aagagaatag acagaattaa gtttacttaa ttctgtctat tctccttaat taatatatgt
      121 aattagtgaa gactataaaa tgttttgttt ccaccccaag cctttggagc agatgcctta
      181 gattgaacac caggtcgagc aacaccacta aaagatgaat atttccctac tgctgttgaa
      241 ccatgatttc ttttattgtg actataataa gaccatacgt aatgttttcc aataccatgg
      301 ctccaaacac caccagctac atgtactgtt gcagcttcag ctttataagt tacacctaat
      361 aaagtgccga ataagattgt actagcaata caactagaaa caaatttctt tttcataaaa
      421 acacctccat ttaatattct ttatttatta tagcaaaaat aaataaaata gagaatgagt
      481 aaaaataatc taaaagtgtt ttttctgctc tgttgcttgt tttgttcatc tatatataat
      541 attaattcta ttatccttaa attaactatc attaaacaac gatatgacaa tgtataataa
      601 atacaatttc actacctata attcaacatt atataaacta aagtatttca aataattaac
      661 tgtattaata ctctcatcat gattgcgtta taaacgttcg ttaatgagaa aaagagagag
      721 catatttacg ctctcttttc ttgtcttatt attaaaaact cataatagat tataatcatc
      781 tgttttgaga cgctgtgaga agctctcagt tgcattgaaa caaagtttat taaatcaaat
      841 cattatcgtt tttaattctg taaatcgtct gccttgttat atttacttct ttcgcaattt
      901 tactaattga ttggccttct tctaacattt ctacaacgcg atgataaata atacgtttct
      961 gtggatcttt agcatttggt gaataaagta aggggcgccc tttatatacg cctttttcct
     1021 tcgcaacttg aattccttgc gcttgtcgac gtttactttc atttctctct tgttctgaaa
     1081 ccattgctaa tatttgaata attaaatctt tcataaactt atccagtaat ggattgccaa
     1141 ccacttcatc ataattacga cctaaacaat caatggattc tactacaaac cgatctccca
     1201 tcctcaaaaa gttaagtgct tcttgaagta ctggtctatt ttcaatagat ttcccagact
     1261 gtttttctgt aaatattttt tcagcacaaa acgtctttaa attttcaagt tgtctttcta
     1321 aattttgatc gttcgatgat actctcgcat agccaacaat cattgatttc catctcataa
     1381 ttaatataca ccctaatcat acgtttcagt tactataaat tcaacaatta tgtcactttg
     1441 cttgatatat gagaattatt taaccttata aatgagaaaa aagcaacgca ctttaaataa
     1501 gatacgttgc tttttcgatt gatgaacacc tataattaaa ctattcatct attatttatg
     1561 attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa
     1621 aataataaag ggaaaatcag gttttgatat caaaattata catgtcaacg ataatagaaa
     1681 atataataca aactataaga tgttatcagt atttattatg catttagaat attaaaattc
     1741 cttcattaca ctcttggcgg tttcacttat caacttatca tttggcttat cacttttatt
     1801 gtctttattc gtaaaaatga ctaaaacaat aggttcagat tggcccttag gataaacaaa
     1861 agcaacatca tttctagaag catatgttat tgcttgacca cttttatcag caaccttata
     1921 gtcttttgga acaccatctt taattaaagt gtctccgttt ttattattaa acattaaatc
     1981 aagtaagaaa tttttatttt ttttgcttaa ttttccattt gcgataagtt tatttaaagt
     2041 cttgccgaaa gcagcaggcg ttgaagtatc ttttttgctc tttggcgaat agtaatttaa
     2101 ttctatctca tatctaactg gatttgttac tttatctcct agttctttta gacgttgttt
     2161 aacttttttg attccaccga tttcttttat aattttattg tttgctgtat tatcactata
     2221 tgtcattgaa gcctcaataa gttcttttaa agtgatgtct tttcctacat atttttctaa
     2281 aataggagaa taagcaacta tatcatcttt gttaatatgt acttttttat ttaacttatt
     2341 ataaggtact tgttctaaca aaatagcact atttatcgct tttgaagtcg aagcataggc
     2401 aaatctctta tctgaattaa attttacttc cttaccactt ttagtatcta aagcataaac
     2461 accaatatga gcattatatt ttttttctaa atcatttaac tctttggcat gtgaactgtt
     2521 tgaattacat gcacttaaaa ctaaagccat tacaattaaa aatattaact ttttcaaaat
     2581 aaaccctccg atattacagt tgtaattttt acaaaacaat aatattacaa ttgtaatatt
     2641 gatgtcaatt gtttattata aatatttgaa agaaggtgtc aaaatggcta aattactaat
     2701 aatgagtgta gtaagctttt gttttatatt tctattgtta gtatttttta ggtacatatt
     2761 aaaacgctat tttaattata gtttaaatta taaagtttgg tatctaactg tgcttgctgg
     2821 attaattcct tttatcccaa ttaaattctc ttttataaaa tttaataatg tgaataatca
     2881 agcgcccaca gttgaaagta agtcacacga cttgaaccat aacataatta ccaccaaacc
     2941 tgttcaagag ttcgcaacag atatccataa gtttaattgg gattcaattg ataatatctg
     3001 cacagttatt tggatagttt tagttattat tttaagtttt aaatttttga aagccttatt
     3061 atatcttaaa tatttaaaga aacagtcact ttatctaaac gaaaatgaaa aaaataaaat
     3121 agatacgata cttttcaacc atcaatataa aaaaaatatt gtgattcgaa aagcagaggc
     3181 tattcaatct ccaataactt tttggtatgg gaaatatatt attttgattc ctagttcata
     3241 ttttaaaagt gtaattgata aaagactaaa atatataata ctgcatgaat atgctcatgc
     3301 taaaaataga gatactttac atttaattat ctttaatatc ttctctatta ttatgagtta
     3361 caatccatta gtacatattg taaaaagaaa gataattcat gacaatgaag tagaagccga
     3421 tagatttgtc cttaataata ttaacaaaaa tgaatttaaa acttatgcgg aatctattat
     3481 ggactccgta ttaaaaacct catttttcaa caaaaatata ttaagccatt catttaatgg
     3541 taaaaaatca ttactcaaaa atagattaat taatataaaa gaagccgatc tgaaaaagca
     3601 gtcaaaatta attctaattt ttatttgtat ttttactttt tttatcatga taattcaaag
     3661 ccagttttta atggggcaat ccttaactga ttataattat aaaaagccct tacaaagcga
     3721 ttaccaaatt ttagatgaaa gtaaaaattt tggttcaaat agtggatctt tcgttatgta
     3781 cagcatgaag aaagacaaat actatattta taatgaaaaa gaaagtagaa aaagatattc
     3841 tcctgattca acttacaaaa tttatttagc actgtttgga cttgaccgtc atattataag
     3901 tgataaaaat tcacgtatgt catggaatca taaacattat cttttcgagt cttggaataa
     3961 ggaacaagat ttaaatacag caatgcaaaa ttcagttaat tggtacttcg aacgtattag
     4021 taatcaaata ccaaagaact atactgcggc tcaactcaag caattaaatt atggtaatga
     4081 aaatttggga agttataaaa gctattggat ggaagatagt ttgaaaatat ctaatcttga
     4141 acaagtaata gtttttaaaa atatgatgga acaaaataac cattttagta aaaaagcaaa
     4201 gaatcaatta tcttcttcat tattgattaa gaaaaatgaa aagtatgaac tgtatgggaa
     4261 aacaggtaca ggtatagtaa acgggaagta taataatggg tggtttgtag gttacgtaat
     4321 tacaaatcat gataagtatt attttgctac acatttatca gatggaaacc cgtctgggaa
     4381 aaatgctgaa ttaataagtg aaaaaatatt aaaaggaatg ggtgttttaa atggccaata
     4441 agcaagttga aatatctatg gctgaatggg atgttatgaa tataatatgg gataaaaaat
     4501 cagtatcagc taatgaaatt gtagttgaaa ttcaaaaata taaagaagtt agtgataaaa
     4561 cgattagaac attaatcaca agactatata aaaaagagat tataaaacga tacaaatcag
     4621 aaaatattta tttttactca tcaaatatta aagaagacga tattaaaatg aaaactgcta
     4681 aaacctttct taataaactg tatggagggg atatgaaaag tttagtgttg aattttgcga
     4741 aaaatgaaga attaaataac aaagaaattg aagaattgcg agatatttta aatgatatta
     4801 gtaaaaagta attagaggta tgtttcaaac ataatacata tgtaaactat gcttgactct
     4861 tattaaatta gttttgttaa tgacatttaa aatatattat taaagatgag agtgtacaga
     4921 aaataaccat tttctgtaca ctcttctttt atgtctaaat aagtaagaat tacataggat
     4981 tatgtgttca aaaacccaac aaaaaatcta tgttcataaa agaccttgta attaagttat
     5041 ggtacactga agatgtatat aggaggtttg tattttgaaa ataggttatg caagagtttc
     5101 aactggatta caaaatttaa atttacagga ggatcgatta aatacatatg gttgtgaaaa
     5161 gatatttaat gaccatatga gtggttcaaa aagtaaaaga cctggtttag ataaagcaat
     5221 tgaatttgcg aggtcaggag atacgattgt tgtttggaga ttagatagat tgggacgtaa
     5281 tatggaggat ttaatcacat tagttaatga gcttaacgaa cgaggtgtaa gtttccatag
     5341 tttagaagaa aatataacaa tggataaatc aagttcaact ggacaattat tatttcattt
     5401 attcgcagca tttgcagaat ttgaacgtaa tttaatttta gagcgttctt cagctggccg
     5461 aattgcagca cgtgctagag gccgatatgg aggcagacca gaaaaattaa atcaaaaaga
     5521 tttaaactta cttaagaccc tttatgataa tggcacgcca attaagacaa ttgcagaaca
     5581 gtggcatgtt tcacgtacaa caatttatcg ttacctcaat aaattggagg acaaggaaga
     5641 tgaaaaacaa ggagaagtat ctaactaatt tttctgaagc caaacgtaaa aaagctactc
     5701 aaaagtacaa tattataaaa ccttttatcc taggcgaaca atctttatca agtatttcta
     5761 aaagtaaagg gattgcgtta agcacccttt ataggtggaa taagtcatat aaagaacaag
     5821 gacttaaagg actaatctat gcgacaagag ctgataaagg aacacgtaaa atagaaccaa
     5881 agataattga tgaaattgaa cgccttgcgc ttatgaataa aagaaatagt atagccacaa
     5941 ttcataggaa aatcactaat tactgtaaag aaaataattt tgatatacca agttataaac
     6001 aaatttatag tgttattaaa gcgatgccta agtctgttat tgatttctct catcagggtg
     6061 aaaaatacta tcaaaataag tacgatttaa tacaaattag agaatcctcg agacccaatg
     6121 aaatatggca agcagaccat actttgctag atatctttat attagaccaa aaaggtaata
     6181 tcaatagacc atggctaact attattatgg atgactatag tcgtgcaatt gcaggctatt
     6241 tcttaagttt tgatgccccc caatgctcaa aacacagcat taacactaca ccaagcaata
     6301 tggaataaaa atgataccaa ttggccagtg tgtggtatac ccgaaaaatt ctacacagat
     6361 catggaagtg actttacttc acatcatatg gaacaagtcg ctattgattt gaaaattaac
     6421 ttgatgtttt cgaaagttgg tgttcctcgt ggtcgtggaa aaatagaacg attctttcag
     6481 acagttaatc aaactttctt agaacaactg cctggatata taaacaataa tgatactcct
     6541 agtaatctaa taaattttca caattttgaa gaaaaattac gttacttttt aattgaagat
     6601 tataatcaaa aagaacatag tactattcat accacgccta tcagtcattg gaatagtaat
     6661 cactttttcc ctaatatgcc aagtagctta gaacaacttg atttattatt gttggaaata
     6721 cctaaatcta gaaaagttca ttcagatggc attcattttc aaggttttcg atacagtaat
     6781 actaatctag aagcttatgt aggtgaatat gtactgattc gttataaccc aaatgatatg
     6841 gctgaaatac gtgtgtttta tagagatgaa tttctttgta ctgctatatg tccagaaata
     6901 gctgattact ccatagatat aaaagacata cagcacgctc gtagtcagag aagaaaacac
     6961 ttaaaacaaa atattgctag tccaagtacc acggaattaa ttaaggaaga aaaaaattgt
     7021 ggttattcac ctcaagaaac gactaaaaac gtcaaaaaat taaagagata tcgtaatgac
     7081 taaaaatcaa aatttcattg aaacgaaaga gtataaacgc tttgctgaat tttgtgatgc
     7141 ttgtattaaa tatcagtata ttggtatatg ttatggtcaa cctggtgtcg gaaaaacttt
     7201 atcgtctagg tattacacaa attgggatac tattgaaaaa caagttaacc atagaggttg
     7261 ggaagattta gctagtaaaa cgaccgatga catactatcg gtggataaaa tattttataa
     7321 tgcaccagca gaaaaacaaa ctaaattaag aaatgattta tatagtatta ctgcaatcat
     7381 tgatttgggt caaaaattac atgttgtaaa taagtatgga catgaacata gtaaacatta
     7441 tagtgatatg tttaaatata ttgatttaat tatagtagat gagattgatc gccttaaagt
     7501 gcaacattta gagcaattaa gagctattta tgatgaacat aatttagcaa tgatatttat
     7561 tggtatgccg ggtatagaga aaaaactatc tcgttatcct caactatatt cgcgtatagg
     7621 ttttgcgcat gaatttgata atctaagtaa agatgagacg catcatatat tagaatataa
     7681 gtggcaagac ttaggatttg atttaaaact cgaagacttt actgattatg aggcaataac
     7741 gacgattatt aaaattacaa aagggaattt taggctgatt catcgtttat ttgcgcagat
     7801 agacagaatt atggatataa atggtttaga taaaataagt acagaagttg tagaaacagc
     7861 tagagatagt ttagttatag gtattcgata atagaaaaag caacgcattt taatatagtg
     7921 cgttgctttt ctcatttatt aggtaaagtc attctcatat atcgagtaaa gtgacaacaa
     7981 ttatatatgt atgattaggg tgtacctaaa aaatacataa attatattac aatattttta
     8041 ttatagaggc taaagtatct taaaaatttg gatagtaggt ttttcaggaa taaaagtata
     8101 gttggcttta atatataatt aagctaagct atcatttata tttactcgat tataattcgc
     8161 acttcaacgt tttaataacg taaaaattaa aacgtagttt catttatctt tagtaatatc
     8221 tttttaaaca ctacagtttc ttcattagtt aaattagcga agtaattatt attaagttga
     8281 taggctttct tttctaatgt tggccataag tcttttgctt tatttgtact gtttattagc
     8341 acagttcttc tattttcatt atcagtttga cgtgttatta gatccttttt ggacatctta
     8401 tctaatattc cagatattgt tgaaggttct aaatttaaag tattaccaat cgctttaggt
     8461 gtattcaaat tactattaat caagcaattc aaaacaccat attctgctgg agtaatatta
     8521 tagggactta ataaactttt aaaatgctta aacactctat tctgagaaat actcaacaaa
     8581 taatttatac aattatctaa ttccacaact aaaacctctc ttaaatcatt agtattgact
     8641 cgcttatcat atcatgataa gttttgtagg aaaatagttt tgcaacaaaa acatacctct
     8701 acaaaacaat tgtgaaagaa ggctaataaa tgttcaatgc ggtagtagtg agaaaaagtg
     8761 catcaaatgt tagttataaa gtagaaaccg ttgatgaatc ttatttatcg aagggtgatg
     8821 tcttagtaaa agtggtatac tcttcaataa attataagga tatgctagct ttacaatata
     8881 acggcggtgt aattagagat tatcctatga ttcctggtat agattttgca ggtattgtcg
     8941 aatcaagttc gaatgataag tttaaagaag gcgataatgt attagttact ggttatgatg
     9001 tcggcgtgac acatacaggt ggattttcag aatatgcaca agtgcctgga gagtggattg
     9061 ttcctattcc ccaagaaatg tctattaaag agtctatggt atatggcaca gccggtttta
     9121 cggcagcatt atcaattttt gaactagaaa aatatggaat gaatatcaaa tcacaacctg
     9181 aaattttagt gtctggggct acaggtggtg tgggcagtgt tgctatacaa attttacgta
     9241 aaattggata tgaaaatatc actgctatcg taagaaaaga aaagcaaata gaaatcgcaa
     9301 aaaaattagg tgcaaaacaa gtaataataa ttgaagatga taataaacca cttaaaaaag
     9361 gtatttttga ttatttccta gatacagtgg ggagtaatgt aacattatac ggtttaaaac
     9421 gtctaaatta tcaaggtgtt gctactgtat gtggaaatgt agcaggaaat tcacttaacg
     9481 ctagtatatt accgtttatt cttagaggta ttaaattaat tggcattgat tctgtatatg
     9541 ttgatcataa tatccgtgaa gatatatgga gtaagttagc aaatgaatgg aatataggaa
     9601 attcacttct atacaatgaa attggatttg aagaattcta taaaacgata gatcagcttt
     9661 taaaaggaca acatttagga agaactattt taaaagtata agtatgagta ttcattaata
     9721 catttactat taattcaatc attttgcatt tctctactaa ccagtgttat aatttatata
     9781 acgaaaatga ttaacaatta atgcaccaat ccccacacta ttgcagtagt gtggggattt
     9841 tttcgatgct ggcttataaa gtcgcctatt aattatttgt tgcgtttact aagccaataa
     9901 gtataatacg caacagcaca gccactgatg actggtgcta tgatgtgaac gaaaa
//
LOCUS       DBNENW010000384         6688 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.guided.135, whole genome shotgun sequence.
ACCESSION   DBNENW010000384 DBNENW010000000
VERSION     DBNENW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.guided.135"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            136..1221
                     /locus_tag="AC1LW8_002887"
     CDS             136..1221
                     /locus_tag="AC1LW8_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIP4774615.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1218..3110
                     /locus_tag="AC1LW8_002888"
     CDS             1218..3110
                     /locus_tag="AC1LW8_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP4774616.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3117..3494
                     /locus_tag="AC1LW8_002889"
     CDS             3117..3494
                     /locus_tag="AC1LW8_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HIP4774617.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3645..4427
                     /locus_tag="AC1LW8_002890"
     CDS             3645..4427
                     /locus_tag="AC1LW8_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HIP4774618.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4553..5284)
                     /gene="erm(A)"
                     /locus_tag="AC1LW8_002891"
     CDS             complement(4553..5284)
                     /gene="erm(A)"
                     /locus_tag="AC1LW8_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HIP4774619.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5342..5401)
                     /locus_tag="AC1LW8_002892"
     CDS             complement(5342..5401)
                     /locus_tag="AC1LW8_002892"
                     /inference="COORDINATES: protein motif:HMM:NF019858.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HIP4774620.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            5793..6455
                     /locus_tag="AC1LW8_002893"
     CDS             5793..6455
                     /locus_tag="AC1LW8_002893"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP4774621.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         2406 a          903 c         1275 g         2104 t
ORIGIN      
        1 tattaaccat agtataataa ataatagtag ttaaagtagt ttaagaggag atattaaagg
       61 gatattttac tttaaataaa aattaacaag gtttataatt tgtagtagtt aaagtattta
      121 aagaggtggg aacatgtgaa ggttcaaagg atagaagtgg agaataagcc gtatccattg
      181 tatttattac tagataaaga ataccagcta atagaaccag taatgaaatt tattaaatac
      241 ttagataata ctggtaagtc tcctaatacc attaaggcat actgctatca tttaaagttg
      301 ctgtacgagt tcatggaaca gagaggtgtt attcttaatg atattaactt tgagttgtta
      361 gcagacttcg taggttggtt gagatatcct tcagcatcaa atgtaattga tcttcagtca
      421 aaaaaagcca taagagaaga aacgacagtg aatacaattt taaatgtagt tatgagtttt
      481 cttgattatt taagtagatt aggagaattt aaatcaattg atgtatttaa acaagcaaag
      541 ggaagaaatt tcaaaggatt tttacatcat gttaataagg gtagatacca aaagaatgtc
      601 ttaaagttaa gggttaaaaa gaaacagata agaacattga gatcaaagga agttaagcaa
      661 attattgatg cttgtcatac gaaaagagat aaattaattt taatgcttat gtatgagggt
      721 ggtttaagaa tcggtgaagt gttatcgctt aggcttgaag atattgtcac ttgggacaat
      781 caaatccatt taacacctag agatgttaat gttaatgaag cttatattaa attaaggaag
      841 gaaagaacaa tacatgtgag taaagaactt atgtcacttt atacagatta cttgatatat
      901 gagtatagtg aggaattgga gcatgattat gtttttattt ccttaaaaga aggctatttt
      961 gggaaaccac taaagtacca aagtgttctt gatctagtta gaagaatagt taaaaggact
     1021 ggaatagaat ttacatcaca tatgcttcgc cacactcacg caacgcagct aattagggaa
     1081 ggatgggatg ttgcgttcgt tcaaaagaga ttaggtcacg cacatgttca gacaacgtta
     1141 aatacctatg ttcatctttc agatcaggat atgaaaaatg agtttaataa atacctcgag
     1201 agaaaggagc ataagaaatg aatgcttcta gtaaaaggaa aattattagt cagagtgaga
     1261 ttagcaaaaa aatagctgta atgaatgaag aaatgcaggg gttttgggct aataatagtt
     1321 gggatataag aaaatgtcca catccttctg ccatagaatt aagtaagaat cctgctttaa
     1381 ggaatcgttg ggttcgtttt gaacgtgtta aaaatctgtg gttaagaaca gaattgaaat
     1441 atttttattt ttaccattta aacaatggaa tatggaatgc aaaaactgtc tggattagaa
     1501 aaggaacagt aattaataaa atgttagatt tcttagattt aaagtatcct agcattactt
     1561 caattactga agttcctatt gaaaaagcaa tgacggagta tagaacttat ttgacaaaac
     1621 ggggtgttag aattaccact actaattata agattactgc taatcaagaa aaaacacctg
     1681 taaaagctaa ttcctactat gttactaatc taaaacaatt tatggagttt tatgagaact
     1741 tttattttga tggagaggag tgggataaag acgtttggga tagacgtaac ttacctttgc
     1801 cagatgataa ggttaaccca acacaatatg aatatacaat taactttaaa gggtttcgga
     1861 atacatattt taaacaactt gtaaaaagat attgtaagtt gagattgaac gtggatagct
     1921 tttcctatgt aagtgatatt gcccaaagac ttaaagagtt ctttaatttt ctggacatga
     1981 aatttaaaca agttcagaga gtacaccaat taacgagagt ggaaattgaa gcatatttaa
     2041 gtgaactaaa catgatggga ataaaaccta gtacaataac tgggaggatc tctatattgg
     2101 aaggactatt tagtaccctt cttaggctag aatgggatga tgttccttcc aaaatattaa
     2161 tttattctga ggactatccg aaaataccaa gagcaaaacc acgctttata gatgaattcg
     2221 tcctagagca attgaacagt catcttgata aattacccga atatatagct acgatgacta
     2281 tgattgttca agaatgtgga atgaggataa gtgaattgtg caccttgaaa aaaggctgtc
     2341 tattagagga caaagatgga gatttctttt taaagtatta tcaatggaaa atgaaaaagg
     2401 agcatatagt tccaatatct aaagaggtag ctttacttat taaagttcgg gaagataaag
     2461 tttcagagga atttccagat agtgaatacc tctttccaag aaaagatgga tcgccattaa
     2521 aacaagaaac atttagaggt gagttaaata aattagctta tgagcaaaat atagtggata
     2581 aatcaggtga gatttataga ttccatgccc atgcctttcg ccatacagta ggaacaagaa
     2641 tgattaacaa cgggatgccc cagcatattg tgcagaaatt tttggggcat gaaagcccag
     2701 aaatgacaag cagatacgct catatctttg atgaaactct aaaaaatgaa tttactaaat
     2761 ttcaggaaaa actggttacc aataatggag atgtgcttga tctagatgaa gataatgaag
     2821 tcgatgatgt agagcttcaa tggttcaaga aaaatataaa tgcacaagtg cttccaaatg
     2881 gttattgtag attgccagta gtagcaggtg gttgtccaca tgcgaatgca tgcttagatt
     2941 gcactcactt ctgtaccagt aagcaattct taccacagca cgaagaacag ttagagcgta
     3001 cagaagagtt attagccata gctaaggata aacaatggca aagacaagta gagactaata
     3061 gccgtgttaa agagcgttta gaacaaatca ttggaagttt gacggggtaa ttatcaatgg
     3121 ataaacaagt tagaaataca acagaaattg tacgtttggc gaagcagaaa tcaaaaaaga
     3181 caagggaaaa agtagacaaa gcgatttcta aattttcgat tgaaggtaaa gttattaatt
     3241 ttaattcaat agcaaaggaa gctaatgttt ctaaatcatg gctttataag gaacacgata
     3301 ttaggcaaag aatcgaatcc cttcgtgagc gtcaaataac agcaaatgta gtctcaaaac
     3361 ccaagaaaag ttctcgttcg gaggaaatcc ttattaaaac cttaaaaaga agagtaatgg
     3421 aattagaaaa agaaaataaa aaattacaga accaaattca aaaattatat ggagatctgt
     3481 ataataaaga ataattatta atctgtagac aaattgtgaa aggatgtact taaacgctaa
     3541 cggtcagctt tattgaacag taatttaagt atatgtccaa tctagggtaa gtaaattgag
     3601 tatcaatata aactttatat gaacataatc aacgaggtga aatcatgagc aatttgatta
     3661 acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat ttatttggaa
     3721 gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta cgcattaaca
     3781 gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact cgaaaaaaac
     3841 taacagaaag actaatgact atatcaggaa agattggaaa tacggattct gttagaccac
     3901 ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct ccaaaaagag
     3961 aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt caggaaccaa
     4021 gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt atttctctat
     4081 ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt cgaagagcaa
     4141 ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt aatgtaattt
     4201 taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg aaagatgtcg
     4261 ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg gatatagcta
     4321 gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca aaggtgaaag
     4381 cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct aattttattg
     4441 caataacagg tgcttacttt taaaactact gatttattga taaatattga acaatttttg
     4501 ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta atttagtgaa
     4561 acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta ttaatattag
     4621 tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga acacgatatt
     4681 cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc tttgaaatca
     4741 atggttgatg tcgctcaaga acaatcaata cagagtctac acttggctta ggatgaaaat
     4801 atagtggtgg tacttttttg agcattttta tatccatctc caccattaat agtaaaccca
     4861 aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata agatagctat
     4921 atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg ttataaggaa
     4981 tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga atatccgttt
     5041 gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga cataagcctc
     5101 catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg gtaaaatgtc
     5161 cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg tgattcaata
     5221 tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg tttttctggt
     5281 tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc attataacca
     5341 attaattttg atttggttga taacgaactt tattaataac aaatatagaa aaagtaccca
     5401 tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt tctactactg
     5461 cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg tatcatcttt
     5521 tcattttaga ataaaccttt atatgtaatt ttgcacttga cacttcagat ggacgttttc
     5581 ttcaaaataa tatgttttga aacttgataa gagaagccac tgtctttact aaaaagtaaa
     5641 ggctgcgata aaggacagtg actttaataa agttgcactg ttgtattgtc tttggataaa
     5701 attgtctaaa gggaagtttt atccccaacg aaaaaacatc taaagtggta agtaatgaaa
     5761 gaatgtcgat aaagtaggaa tgggggatct ttttgaatac aatagaatca cagtttgata
     5821 aggttgcaga agattacgat ttcgtgaatg agcttttgaa tgattattca ttctttgtgt
     5881 ctaatatgtc tccaaagaaa ggcagagcat tagatatcgg atgtggctcg ggtttgttag
     5941 tggagaaatt agcaagttat tatgatgaag tggtagggat tgatatttct aatcaaatgc
     6001 tcgatcttgc caaatctaaa cgtcaactaa caaatacggt ctatctgaat atgaatgcag
     6061 aacaacttaa ttttaatgag aagtttgatt ttattgtaag ccgaacaacc tttcatcatt
     6121 tggatgatat agccagcgta atacagcaaa tgaaggaact gttgaatgag gaaggaagaa
     6181 tagttattct tgataatgta tctgaagtcg agacaccgcc tacctatgtt tataaattag
     6241 gggcaatcca agagttttta ccgcattgtt tcaaatttgg aataaaaaat gcgattagaa
     6301 tttataatca caacacatct aaatcatggc ttgagcattt agcttcagat aagtacctat
     6361 ctgaacaaaa ttattacgat ttatatgaaa agttgttgcc tggatgccaa ttccataaaa
     6421 tgggttgggc aatgggggtt gtctggacaa aataacttaa agctgtggct ttgaaaagtt
     6481 gaatttgtga ttgttttcag agctatcggg tgctttagta taaataacaa tgaataaaaa
     6541 atcagcctgt atcttatgtt actattaagt aatttaagat acaggtttta ctatttttga
     6601 aataagtacc ttccacttca aatacatgta ttcaaagtga aagtattgat ttaaatgatt
     6661 cttttgtaat ttactcttga taagatgt
//
LOCUS       DBNENW010000385         5334 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.guided.159, whole genome shotgun sequence.
ACCESSION   DBNENW010000385 DBNENW010000000
VERSION     DBNENW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.guided.159"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..254
                     /locus_tag="AC1LW8_002894"
     CDS             <1..254
                     /locus_tag="AC1LW8_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HIP4774622.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..487)
                     /locus_tag="AC1LW8_002895"
                     /pseudo
     CDS             complement(245..487)
                     /locus_tag="AC1LW8_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505894.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(477..1463)
                     /locus_tag="AC1LW8_002896"
     CDS             complement(477..1463)
                     /locus_tag="AC1LW8_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HIP4774623.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="AC1LW8_002897"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="AC1LW8_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HIP4774624.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..4043)
                     /locus_tag="AC1LW8_002898"
     CDS             complement(3615..4043)
                     /locus_tag="AC1LW8_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIP4774625.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(4140..4883)
                     /locus_tag="AC1LW8_002899"
     CDS             complement(4140..4883)
                     /locus_tag="AC1LW8_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HIP4774626.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1877 a          861 c          787 g         1809 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata atttaaatga tattgaaagt gtatgcatgc cagatgcaat gataccttta
     3901 aatctacttt gttctgcttt ttctttatct atatgcatat attgaggatc aaaagttgtt
     3961 gcaaattgga taatttcttc ttctgtaata tgaaggcttt ttgttttgaa tgtttctcct
     4021 actataaaat catcgtattt catatatgtc tctctttctt attcaaatta attttttagt
     4081 atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt aagactcacc tctaactttc
     4141 tattgagaca aatgcaccat tttatctgca ttgtctgtaa agataccatc aactccccaa
     4201 ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc atacgttcaa ttcataaccc
     4261 gcttctttta ccatttttac ttttgcttta gtaagtttgg catcttcagt gtttactatt
     4321 ttagcattac agtaatctaa aagtgttctc cagtcttcac gaaacgaagt tgtatggaat
     4381 ataactgctc tgttatattg tggcatgatt tcttctgcaa gtttaacaag cacaacatta
     4441 aagcttgaaa tgagcacttc ttgattctga tttaagtttg ttaattgttc ttccacttgc
     4501 ttaaccatac ttttagaaag tgctagtcca ttcggtccag taataccttt taattctaca
     4561 tttaaattca tattatattc atttgctatt tttactacat catcgaaagt tggcaaatgt
     4621 tcatctttga atttttcacc aaaccaagat cctgcagaag catctttaat ttcatcataa
     4681 ttcaattcag ttatttcccc ggacatattt gtagtccgtt ctaaataatc atcatgaatg
     4741 ataatcagtt gttcatcttt tgtaattgca acatctaact ccaaccagtt tataccttct
     4801 acttctgaag cagctttaaa tgatgcaatt gtattttccg gagctttact aggtaatcct
     4861 ctatgtccat atacagttag catattacct ctccttgcat ttttattttt ttaattaacg
     4921 taactgtatt atcacattaa tcgcactttt atttccatta aaaagagatg aatatcataa
     4981 ataaagaagt cgatagattc gtattgatta tggagttaat ctacgtctca tctcattttt
     5041 aaaaaatcat ttatgtccca agctccattt tgtaatcaag tctagttttt ctgtacccct
     5101 tatctgcaat tttacttagg attgctttta acttacccct tatcagcaat tttactgaga
     5161 actgctttta acgcacctct tatctgcaat tttgcctaga actgctttta acgtacctct
     5221 tatctgcaat tttactgaga actgctttta acttacccct tatcagcaat tttgcatgga
     5281 attgctttta acgtacctct tatctgcaat tttacttaga actgctttta acaa
//
LOCUS       DBNENW010000386         4021 bp    DNA     linear   BCT 07-APR-2026
DEFINITION  TPA_asm: Staphylococcus aureus strain HS174
            SAMD01831786-rid29412923.guided.182, whole genome shotgun sequence.
ACCESSION   DBNENW010000386 DBNENW010000000
VERSION     DBNENW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD01831786
            Sequence Read Archive: DRR917898
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003169199.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003169199.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2026 15:42:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,906
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,761
            CDSs (with protein)               :: 2,761
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 52 of 82
            Pseudo Genes (incomplete)         :: 30 of 82
            Pseudo Genes (internal stop)      :: 21 of 82
            Pseudo Genes (multiple problems)  :: 20 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD01831786-rid29412923.guided.182"
                     /strain="HS174"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="Japan: Hiroshima"
                     /lat_lon="34.2329 N 132.2707 E"
                     /collection_date="2022-05-27"
     gene            <1..716
                     /locus_tag="AC1LW8_002900"
     CDS             <1..716
                     /locus_tag="AC1LW8_002900"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIP4774627.1"
                     /translation="NNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQM
                     KQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRH
                     IVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTH
                     LIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYE
                     DLEIVDYESHPAIKAPIAV"
     gene            916..1395
                     /locus_tag="AC1LW8_002901"
     CDS             916..1395
                     /locus_tag="AC1LW8_002901"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIP4774628.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1410..2249
                     /gene="fakB2"
                     /locus_tag="AC1LW8_002902"
     CDS             1410..2249
                     /gene="fakB2"
                     /locus_tag="AC1LW8_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HIP4774629.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2335..2868
                     /gene="msrA"
                     /locus_tag="AC1LW8_002903"
     CDS             2335..2868
                     /gene="msrA"
                     /locus_tag="AC1LW8_002903"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIP4774630.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2861..3289
                     /gene="msrB"
                     /locus_tag="AC1LW8_002904"
     CDS             2861..3289
                     /gene="msrB"
                     /locus_tag="AC1LW8_002904"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIP4774631.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3301..3801
                     /locus_tag="AC1LW8_002905"
     CDS             3301..3801
                     /locus_tag="AC1LW8_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP4774632.1"
                     /translation="MFKKLFGKGKEVQKDIAIYTPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3801..>4021
                     /locus_tag="AC1LW8_002906"
     CDS             3801..>4021
                     /locus_tag="AC1LW8_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIP4774633.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         1457 a          593 c          736 g         1235 t
ORIGIN      
        1 ataataatat ttggaacgaa tgggcttttg aaaattatat aaaatcagac gagtataacg
       61 gtccagatat gacagatttc gggcatcgtg cattgagtga tcctgaattt aacgaacaat
      121 ataaagaaca aatgaaacaa tttaagcaac gtattcttga agatgatact tttgcgaagc
      181 aattcggtga tttaggaaat gtttatggta aacaatggcg agattgggtt gataaagatg
      241 gtaatcattt tgatcaactt aaaacagtaa ttgaacaaat taagcataat ccagattcaa
      301 gacgacacat cgtatctgca tggaatccaa cagaaattga tacaatggca cttccgcctt
      361 gtcataccat gttccagttt tatgtccaag atggtaagtt aagttgccag ttataccaac
      421 gtagcgcaga tatcttttta ggtgtgccat ttaatatcgc aagctacgct ttattgacac
      481 accttattgc caaagaatgt ggacttgaag taggagaatt tgtgcataca tttggagatg
      541 cacatattta ttcaaatcat attgatgcga ttcaaacaca attagcacgt gaaagcttca
      601 atcctccaac attaaaaatt aacagtgaca agtctatttt cgacataaat tatgaagatt
      661 tggaaattgt tgactatgaa tcacatccag caataaaagc tccaatagca gtgtagtcat
      721 tgcatagtta gctaaccata tagacatcaa aatgacatca tagtattttc aagtgcaaaa
      781 aagtactttt ttgtgttaaa cgttttcata aattatgcaa aatcattatt tctatcacac
      841 tttatgataa aaattgtgtt aaattaaaga taacttagta ataaaaaatg aaatgataga
      901 agaaggagga taattatgac tttatccatt ctagttgcac atgacttgca acgagtaatt
      961 ggttttgaaa atcaattacc ttggcaccta ccaaatgatt tgaaacatgt taaaaaatta
     1021 tcaacaggtc atactttagt aatgggtcgt aagacatttg aatcgattgg taaaccacta
     1081 ccgaatcgtc gaaatgttgt acttacttca gatacaagtt tcaacgtagt gggcgttgat
     1141 gtaattcact ctattgaaga tatttaccaa ctaccgggcc atgttttcat atttggaggg
     1201 caaatattat ttgaagaaat gattgataaa gtggacgaca tgtatattac tgttattgaa
     1261 ggtaaattcc gtggtgatac gttctttcca ccttatacat ttgaagactg ggaagttgcc
     1321 tcttcagttg aaggtaaact agatgagaaa aatacaattc cacatacctt tctacattta
     1381 attcgtaaaa aataaggggg aaaacgacca tgacaaaaca gattatagta acagactcaa
     1441 catccgattt atctaaagaa tacttagaag caaacaacat tcatgtaatt cctttaagtt
     1501 taactattga aggagcttca tacgttgacc aagtagatat tacatcagaa gaatttatta
     1561 atcatattga aaatgatgaa gatgtaaaga caagtcagcc agccataggt gaatttatat
     1621 ctgcttatga agaactagga aaagatggct ctgaaatcat aagtattcat ctttcttcag
     1681 gattaagtgg tacatataac actgcttacc aagcaagtca aatggtagat gctaatgtaa
     1741 ctgttattga ttcaaaatct atttcttttg gtttagggta tcaaatacaa cacctagtag
     1801 agcttgtaaa agaaggtgtc tcaacttctg aaatagttaa aaagttaaat catttaagag
     1861 aaaacattaa attatttgta gttatagggc aattgaatca attaattaaa ggtggcagaa
     1921 ttagtaaaac aaaaggtttg attggtaatc ttatgaaaat taaaccaatt ggtacactag
     1981 atgatggtcg cttagagctt gtgcacaatg cgagaactca aaattctagt atccaatact
     2041 tgaaaaagga aattgctgaa tttataggag atcatgaaat caaatccatt ggtgtcgcac
     2101 atgctaacgt cattgaatat gttgataaat tgaagaaagt ttttaatgaa gcttttcatg
     2161 tgaataatta cgatataaat gtaactacac cagttatttc tgcacatact ggtcaaggtg
     2221 cgattggcct cgtagtcctt aagaagtaaa tttaatcttt tcagtgttaa ttacttccat
     2281 ttcaatcctt tatagactaa atttataatt agatagatag aggaggtaat tcatatgaca
     2341 aaagaatatg caacattagc aggaggatgt ttctggtgca tggttaaacc atttacatca
     2401 tatccaggca tcaagtcagt cgtatctggt tatagtggcg gtcatgttga caacccaact
     2461 tatgaacagg tatgtacgaa tcaaaccggc catgtcgaag cagtacaaat tacgtttgat
     2521 ccagaggtta cttcctttga aaatatatta gacatatatt tcaaaacatt tgacccaact
     2581 gatgatcaag ggcaattttt cgatagaggc gaaagctatc aaccagtcat tttctatcat
     2641 gatgaacatc agaaaaaggc tgctgagttt aaaaagcaac aattaaatga acaaggtatt
     2701 ttcaagaaac cagtgattac acctattaaa ccatataaaa atttctatcc agctgaagac
     2761 taccatcaag attattacaa aaagaacccg gtacattatt accaatatca acgtggttca
     2821 ggtagaaaag cgtttataga atcacattgg gggaatcaaa atgcttaaaa aagataaaag
     2881 tgaactaaca gatatagaat atattgttac acaagaaaac ggcactgaac caccatttat
     2941 gaatgaatat tggaatcatt ttgctaaagg aatttatgta gataaaattt ctggtaaacc
     3001 tttatttaca tctgaagaaa agtttcattc tgaatgtgga tggcctagct tttccaaagc
     3061 gcttgatgac gatgaaatta tagaattagt cgacaaatca tttggtatgt tgagaactga
     3121 agtgcgttca gaagaatcaa atagtcattt aggacatgtc tttaatgatg gacctaaaga
     3181 aagtggcggc ttaagatact gtatcaattc cgctgcaatt caatttattc catatgaaaa
     3241 gttagaagaa ttgggttatg gcgatttaat atcacatttt gataagtagg agtggaaagt
     3301 atgtttaaaa aattattcgg aaaaggcaaa gaagttcaga aagatatcgc aatttataca
     3361 ccattaactg gagaattcgt gaaaattgaa gatattccag atcctgtatt cgcacaaaaa
     3421 atgatgggcg aaggttttgg tattaatcca actgaaggag aagttgtgtc tccaattgca
     3481 ggacgtgttg acaatgtctt tccaactaag catgcgattg ggttaaaagc agataacgga
     3541 ttagaattat tagttcatat cggtttagac acagttcaat tagacggtga aggatttgaa
     3601 gtgttagtgt ctagtggtga cgaagttaac gtcggagatc cattagtaag attcaacctt
     3661 gaatatatta ataataacgc taaatctgta atttcaccaa ttataattac taacactgat
     3721 caagcagctt caattaatat ttatgatgaa aatgctgtga ttaaaggtga aacaaaagtg
     3781 attgatgtga caatgaacta atgaaaaact attcgtttta tcaatttgtc atgacagttc
     3841 gtggtcgaca cgacgataaa ggtcgtctag cagaagagat atttgacgat cttgctttcc
     3901 caaaacacga tgatgatttt aacatactgt ctgattatat tgagacacat ggtgatttca
     3961 cattgccaat gtctgtattt gatgatttat atgaagaata tacggaatgg ctaaaatttt
     4021 a
//
